LOCUS       DATRTN010000001        74706 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.001, whole genome shotgun sequence.
ACCESSION   DATRTN010000001 DATRTN010000000
VERSION     DATRTN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 74706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74706
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.001"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(10..705)
                     /locus_tag="VBE22_000001"
     CDS             complement(10..705)
                     /locus_tag="VBE22_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO7201483.1"
                     /translation="MSKKSHRQYQIYEGLRCAVALCFISGYINAFTYVTQGKRFAGVQ
                     TGNLLSFAIHLSNKHYSQALAFLLPIMVFMLGQSFTYFMNRWANKHQLHWYLLSSFAL
                     TQVAIVTIILTPFLPSSFTVAGLAFFASIQVDTFKSLRGAPYANMMMTGNIKNAAYLL
                     TKGLYEKNSDIFLIARNTIIIIGGFIFGVVCSTYFSSKLGEWSLSLILIPLLYVNLLL
                     GHEFYNLQVERHS"
     gene            1041..2258
                     /locus_tag="VBE22_000002"
     CDS             1041..2258
                     /locus_tag="VBE22_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7201484.1"
                     /translation="MHSTIYKCHHCRYLIYKSFKDIFPFALIFSWIEILQKLVISDDS
                     FLAQTTKSSIYKNEWLYTVSNTLYDLSHLLIYFMASYFVASLVWRGLSNQTKESFSAS
                     LLGFATVWLLLTSVVPGTDYHYPSQPIWLMLALISYLFIFLTRLLQNRHHIFQYSMLA
                     ILLLISSILSHILYRYPNIDLEYLIQNHFSQWLGDGPHQLIPIMLWSILAIFLTLSGL
                     FVPDILFRPYSYFSVVSENLNAALSQHTDKIPYLYTFYTVKNSFAMFGGIGILLSLFL
                     AVLYESRKLQSKNYYKLTLLTLTPLIFDQNLPFLVGLPVILQPILFIPMVLTTIFAEA
                     FGALMLYLKFVDPAVYTVPSGTPSLLFGFLASNGDWRYLPVTAIILVVGFFIYRPFVK
                     IAFAKEEQYEKIT"
     gene            2242..3084
                     /locus_tag="VBE22_000003"
     CDS             2242..3084
                     /locus_tag="VBE22_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7201485.1"
                     /translation="MKKLLRWLPPVLFIIILIGMTILGKSYINKVTAHKIKLYNSRMT
                     PTILIPGSSATQERFNSMLAQLNQMGEKHSVLKLTVKKDNSIIYNGQISGNDHKPYIV
                     IGFENNEDGYSNIKKQTKWLQIAMNDLQKKYKFKRFNAIGHSNGGLSWTIFLEDYYDS
                     DEFDMKSLLTMGTPFNFEESNTSNHTQMLKDLISNKGNIPSSLMVYNLAGTNSYDGDK
                     IVPFASVETGKYIFQETAKHYTQLTVTGNNATHSDLPDNPEVIQYVAEKILKNEKGKL
                     PKPH"
     gene            3161..3988
                     /locus_tag="VBE22_000004"
     CDS             3161..3988
                     /locus_tag="VBE22_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEO7201486.1"
                     /translation="MKLISWNIDSLNAALTSESTRALMSRQVIDTLVAEDADIIAIQE
                     TKLSAKGPTKKHLEVLETYFPEYDLVWRSSVEPARKGYAGTMFLYRKGLNPIVSFPEI
                     DAPTTMDNEGRIITLELENCYITQVYTPNAGDGLKRLGDRQIWDIKYAEYLATLDSQK
                     PVLATGDYNVAHKEIDLANPSSNRRSAGFTDEERQGFTNLLAKGFTDTFRYLHGDVPN
                     VYSWWAQRSRTSKINNTGWRIDYWLTSNRVADKITKSEMIHSGDRQDHTPIILEIEL"
     gene            complement(4029..4385)
                     /locus_tag="VBE22_000005"
     CDS             complement(4029..4385)
                     /locus_tag="VBE22_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEO7201487.1"
                     /translation="MYTFYEYPKCTTCRSAKKELTELGLTFEAIDIKSNPPKASLLKE
                     LLENSPYDLKKFFNTSGNSYRELGLKDKFDDLTLDQALDLLASDGMLIKRPLLVKDNK
                     ILQIGYRTKYKDLNLV"
     gene            complement(4387..4863)
                     /locus_tag="VBE22_000006"
     CDS             complement(4387..4863)
                     /locus_tag="VBE22_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEO7201488.1"
                     /translation="MLYQEFYQSPLGEIRLLADNLGLSGLYFVGQKYDMLAVNQEEIV
                     NMSNSYTLLGKKWLDAYFSQQNLPSIPLSLRGTAFQTRVRQELQKIPFGDTKTYGELA
                     KELNCPSAQAVGGAIGKNPISLIIPCHRVLGRYGQLTGYAGGLERKSWLLEYEKEK"
     gene            complement(4919..6100)
                     /locus_tag="VBE22_000007"
     CDS             complement(4919..6100)
                     /locus_tag="VBE22_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEO7201489.1"
                     /translation="MVFSVKTFNNINQIGLQELGNRFQIDGDMSENPDAYIIRSQNLH
                     NQDFPSNLKAIARAGAGTNNIPIEEASAQGIVVFNTPGANANAVKEAVIAALLLSARD
                     YLGANRWVNTLTGTDIPKQIEAGKKAFAGNEIAGKKLGVIGLGAIGARIANDARRLGM
                     TVLGYDPYVSIETAWNISSHVQRVKEIKDIFETCDYITIHVPLTNETKHTFDAEAFSI
                     MKKGTTIINFARAELVNNQELFKAIETGVVKRYITDFGDKELLNQKGITVFPHVGGST
                     DEAELNCAIMASQTIRCFMETGEITNSVNFPNVHQIQTAPFRITLINKNVPNIVAKIS
                     TAVSELGINIDNIINRSKGDYAYTLIDLDETDNNKISTLIEEFEGDENIVRVRLIAKQ
                     Q"
     gene            complement(6162..6710)
                     /locus_tag="VBE22_000008"
     CDS             complement(6162..6710)
                     /locus_tag="VBE22_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7201490.1"
                     /translation="MQVRLAFPNEIDQIMLLIEEARAEIAKTGSDQWQKEDGYPNRND
                     IIDDILNGYAWVGIEDGMLATYAAVIDGHEEVYDAIYEGKWLHDNHRYLTFHRIAISN
                     QFRGRGLAQTFLQGLIEGHKGPDFRCDTHEKNVTMQHILNKLGYQYCGKVPLDGVRLA
                     YQKIKEKGETSIYREIDERNPM"
     gene            complement(6779..7870)
                     /gene="serC"
                     /locus_tag="VBE22_000009"
     CDS             complement(6779..7870)
                     /gene="serC"
                     /locus_tag="VBE22_000009"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEO7201491.1"
                     /translation="MTIYNFSAGPAVLPKPVLVKAQSELLNYQGSSMSVLEVSHRSKE
                     FDDIIKGAERYLRDLMGIPDNYKVIFLQGGASLQFSMIPLNIARGRKAYYHVAGSWGK
                     KAYTEAVKLSKTIPFEPILLASSEESVYDYIPEFDEKEIDPEAAYVHVTTNNTIEGTS
                     LYDIPKTNGVPVIADMSSNILAVKYKVEDFAMIYAGAQKNIGPAGVTVVIIREDMINE
                     EPTLSSMLDYKIQSDASSLYNTPPAYSIYIAKLVFEWVKSLGGVDAMEKANREKSGLL
                     YDYIDSSEFYSNPVRDKKSRSLCNIPFITINKDLDEKFVKEATERGFKNIKGHRSVGG
                     MRASLYNAFPKQGVIELIDFMKTFEAENA"
     gene            8003..8638
                     /locus_tag="VBE22_000010"
     CDS             8003..8638
                     /locus_tag="VBE22_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEO7201492.1"
                     /translation="MILREFCAENLTDLTRLDKAIISRVELCDNLAAGGTTPSYGVIK
                     EANQYLHEKGISVAVMIRPRGGNFVYNDLELRIMEEDILRAVELESDALVLGILTSNN
                     HIDTEAIEQLLPATQGLPLVFHMAFDVIPKSDQKKAIDQLVALGFTRILLHGSSNGEP
                     IIENIKHIKALVEYANNRIEIMVGGGVTAENYQYICQETGVRQAHGTRITQ"
     gene            complement(8908..9777)
                     /gene="rsmI"
                     /locus_tag="VBE22_000011"
     CDS             complement(8908..9777)
                     /gene="rsmI"
                     /locus_tag="VBE22_000011"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEO7201493.1"
                     /translation="MEMQVQKSFKSNTHYGTLYLVPTPIGNLDDMTFRAIRILREVDF
                     ICAEDTRNTGLLLKHFDITTKQISFHEHNAYDKISGLIDLLKEGRSLAQVSDAGMPSI
                     SDPGHDLVKAAIEGGIPVVSIPGASAGITALIASGLAPQPHIFYGFLPRKKGQQITFF
                     ETKKDYPETQIFYESPFRVSDTLKHMKEIYGDRQVVLVRELTKLYEEYQRGTISQLLG
                     HIEKVPLKGECLIIVDGKRDTERVKDSSQQDPLVLVKEYIANGDKTNQAIKKVAKELN
                     LNRQELYASFHDL"
     gene            complement(9777..10103)
                     /gene="yabA"
                     /locus_tag="VBE22_000012"
     CDS             complement(9777..10103)
                     /gene="yabA"
                     /locus_tag="VBE22_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEO7201494.1"
                     /translation="MDKKDLFDAFDDFSQNLLVGLSEIETMKKQIQKLLEENTVLRIE
                     NGKLRERLSVIEAETETAVKNSKQGRELLEGIYNDGFHICNTFYGQRRENDEECAFCI
                     ELLYRD"
     gene            complement(10134..10997)
                     /locus_tag="VBE22_000013"
     CDS             complement(10134..10997)
                     /locus_tag="VBE22_000013"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEO7201495.1"
                     /translation="MDLKRTQPKLLEKFNTILQSDRMSHAYLFSGNFASLDMALYLAQ
                     SQFCEKRQSGLPCQKCRACRLIANGEFSDVKIIEPQGQLIKTETIKELTKDFSRSGFE
                     GKSQVFIIKDCEKMHVNAANSLLKFIEEPQSSSYVILLTNDENNVLPTIKSRTQIFRF
                     LKQLDMLVHQAEQAGLLKSQASLLAQVADDPKHLEILLANKKLLDYLNLSQQFVTTLA
                     KDRQTAYLEVSRLTSQVVDKNDQAFVFQWLTIMLAKEGQLYDLENTYRAQQMWKSNVS
                     FQNSLEYMVLS"
     gene            complement(11017..11652)
                     /locus_tag="VBE22_000014"
     CDS             complement(11017..11652)
                     /locus_tag="VBE22_000014"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEO7201496.1"
                     /translation="MKKGLMISFEGPDGAGKTTVLEAVLPLLREKLSQDILTTREPGG
                     VTISEEIRHIILDVKHTQMDKKTELLLYMAARRQHLVEKVLPALEEGKIVLMDRFIDS
                     SVAYQGSGRGLDKSHIKWLNDYATDSHKPDLTLYFDVPSEVGLERIQKSVQREVNRLD
                     LEQLDMHQRVRQGYLELADSEPNRIVTIDASQQLDEVIAETFSIILDRINQ"
     gene            complement(11741..12400)
                     /locus_tag="VBE22_000015"
     CDS             complement(11741..12400)
                     /locus_tag="VBE22_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO7201497.1"
                     /translation="MPVKDFMTKKLVYVSPDTTVAEAADLLREHHLRRLPVVENDQLL
                     GLVTEGTMAEAQPSKATSLSIYEMNYLLNKTKIRDIMIKDIVTVSQYASLEDAIYLMM
                     SRKIGVLPVVDNGQLYGIITDRDVFKAFLEIAGYGQEGYRLVILADEGIGVLSKVLNR
                     LSSANLSVKRLVIIERKAGKKAVEIQLEGYADKDVLKQELVFDDVIVETLEKTVKKPL
                     S"
     gene            complement(12419..13129)
                     /locus_tag="VBE22_000016"
     CDS             complement(12419..13129)
                     /locus_tag="VBE22_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7201498.1"
                     /translation="MTMLKVENLSIHYGVIQAVNDVSFEVNQGEVVTLIGANGTGKTS
                     ILRTISGLVRPSQGSISFMGKPIHKLAARKIVGNGLAQVPEGRHVFSSLSVMENLEMG
                     AFLQKDREQNQKMLKKVFDRFPRLEERKNQDAATLSGGEQQMLAMGRALMSRPKLLLL
                     DEPSMGLAPIFIQEIFNIIEDIKKQGTTVLLVEQNANKALTIADKAYVLETGKVVLSG
                     TGKELLVSDQVRKAYLGG"
     gene            complement(13129..13893)
                     /locus_tag="VBE22_000017"
     CDS             complement(13129..13893)
                     /locus_tag="VBE22_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7201499.1"
                     /translation="MALLEVKNLSKHFGGLTAVGDVSMKLHKGELIGLIGPNGAGKTT
                     LFNLLTGVYLPSKGTISLDGKILNGRKPAKIASLGLGRTFQNIRLFKNMTVLDNVLVG
                     LSNHHLSHPIASFLRLPKYYHSEKALRKKALELLEIFGLKAYQDALAKNLPYGKQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLISQIKSDFDITIMLIEHDMNLVMQV
                     TERIYVLEYGRLIAHGTPEEIKNNKRVIEAYLGGEL"
     gene            complement(13894..14847)
                     /locus_tag="VBE22_000018"
     CDS             complement(13894..14847)
                     /locus_tag="VBE22_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO7201500.1"
                     /translation="MKENMKSILSWLAIVVLGYLLISVLISMGIFNLYHIQIIETIGI
                     NVILAVGLNLIVGCSGQFSLGHAGFMAIGAYAVAIIGVKMPTYVGFLIAILVGTLVAG
                     GIALGVGIPTLRLKGDYLAIATLGVAEIIRILLVNGGDITNGAAGIMGIPPFTTWSLV
                     YGVAVVSLILAMNFLRSPLGRNTIAIREDEIAAESMGVDTTKVKVIVFVFGAILASIA
                     GSLQAGYVGTVMPKDFSFMMSVNVLIIVVLGGLGSMTGTVLAAILLGLLNMLLQDYAS
                     VRMIIYALALILIMIFRPSGLLGTKELTLSHLFRKTSKGGN"
     gene            complement(14850..15719)
                     /locus_tag="VBE22_000019"
     CDS             complement(14850..15719)
                     /locus_tag="VBE22_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO7201501.1"
                     /translation="MLQQLINGLILGSIYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FMGYYLINHLHLNFFLALLIAMLGSAFLGAVIEYLAYRPLRKSTRIAALITAIGVSFL
                     LEYGMVYLVGADTRAFPQAIHTVKYNLGPITITNVQLIILGIALLLMLTLQFIVQKTK
                     MGKAMRALSVDSDAAQLMGINVNRTISFTFALGSALAGAGGVLIGLYYNSVQPLMGVT
                     PGLKAFVAAVLGGIGIIPGAAIGGFVIGILETLATALGVSDFRDGIVYAILILIFLIR
                     PAGILGKNIKEKV"
     gene            complement(15825..16991)
                     /locus_tag="VBE22_000020"
     CDS             complement(15825..16991)
                     /locus_tag="VBE22_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7201502.1"
                     /translation="MEKRLSLGALVLASTVLLAACGNVGGGASSTGTKIGKDIKVGYN
                     WELSGNVSSYGNSMKNGADLAVKEINAAGGVGGKKLKVLSQDNKSENAEAATVATNLV
                     TKGANVIIGPATSGAAASSTPKVNAAAVPMIAPAATQDNLVYGSDGKTLNQYFFRATF
                     VDNYQGKLLSQYATDNLKAKKVVLFYDNSSDYSKGVAKSFKESYSGKIVDSMTFSAGD
                     TDFQASLTKLKGKEYDAIVMPGYYTETGLIVKQARDLGISKPVLGPDGFDSPKFVQSA
                     TPVGASNVYYLTGFTTQGSTKAKAFHDHYVKAYGEEPSMFSALSYDAVYMAAKSAKGA
                     KTSIDLKKALAKLKDFKGVTGKMSIDKNHNVVKSAYVVKLDDGKTSSVNIISAK"
     gene            complement(17145..17390)
                     /locus_tag="VBE22_000021"
     CDS             complement(17145..17390)
                     /locus_tag="VBE22_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HEO7201503.1"
                     /translation="MNKQERLGMSVYLYYNRDARKLYKYGDVHYHSRRLRYLVIYVNK
                     EDIVSVSKEIKHLKFVKDVRLSAIDDIDQDFVGNLYR"
     gene            complement(17490..18080)
                     /locus_tag="VBE22_000022"
     CDS             complement(17490..18080)
                     /locus_tag="VBE22_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEO7201504.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGQVEDNMANSIIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKSDVQTIVMGMAASMGTIIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQSDMAIAAEHLLKTRHTLEKILADNSGQ
                     SIEKVHDDAERDRWMSAQETLDYGFIDAIMENNNLQ"
     gene            complement(18205..18834)
                     /gene="upp"
                     /locus_tag="VBE22_000023"
     CDS             complement(18205..18834)
                     /gene="upp"
                     /locus_tag="VBE22_000023"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEO7201505.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTTTSTKDFRELVDEIAMLMGYEV
                     SRDLPLEDVEIQTPVATTVQKQLAGKKLAIVPILRAGIGMVDGFLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGAASIKFVCL
                     VAAPEGVAALQEAHPDVDIYTAALDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(18944..20113)
                     /locus_tag="VBE22_000024"
     CDS             complement(18944..20113)
                     /locus_tag="VBE22_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7201506.1"
                     /translation="MITYDFTSLPERFSSNAIKWKAVQKDQEILPLWIADMDFPIFPE
                     MSEAIEDFSHQMVFGYDSPKDSLYQAISNWEVQEHGYQFDKKSLLLIDGVIPAISVAI
                     QAFTKEGDAVLINTPVYPPFARTIKYNNRHLVSNSLLNNNQYFEIDFKQLEKDIIENN
                     VKLYIFCSPHNPGGRVWTKGEIQKIGDICKRYNVILVSDEIHQDLVLFDNVHHSFNTV
                     DSSFKEFSVILSSATKTFNIAGTKNSFAIIENEKLRSDFKKRQIANNQQEISSLGLLA
                     TEVAFTKGKKWLKALKMELEGSIEYLYEQLTQKTHIKVMKPEGTYLVWLDFSAYNLTH
                     LEIQEKLRYDAKLILNDGLTFGKEGKKHARINVAAPRSVIEEAVLRLVTNFRKND"
     gene            complement(20249..20797)
                     /locus_tag="VBE22_000025"
     CDS             complement(20249..20797)
                     /locus_tag="VBE22_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEO7201507.1"
                     /translation="MNIETLTQKNHRSDSGRNHIVLFEPQIPANTGNIARTCAATNAP
                     LHIIRPMGFPIDDKKMKRAGLDYWDKLDVSFYDSLEEFMLSCRGKVHLISKFADKVYS
                     DENYNDDQDHYFMFGREDKGLPETFMREHAEKALRIPMNDEHVRSLNVSNTVCMIVYE
                     ALRQQSFPNLELSHTYENDKLK"
     gene            complement(20970..22322)
                     /locus_tag="VBE22_000026"
     CDS             complement(20970..22322)
                     /locus_tag="VBE22_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEO7201508.1"
                     /translation="MENHNSIKQTYGLMTTIAMIVGVVIGSGIYFKVDDILKFTGGDV
                     FLGMVILVLGSFSIVFGSLSISELAIRTSESGGIFSYYEKYVSPALAATLGLFASFLY
                     LPTLTAIVSWVAAFYTLGESSSLESQIILAAVYILALSLMNIFAKRIAGGFQSLTTFV
                     KMIPLVLIALIGAFWSDKAPQLPQHLTAIQPSNVGWSWVSGLVPLYFAYDGWTIFVSI
                     APEVKNPKKNLPLAFVIGPALILLSYLAFFYGLTQILGASFIMTTGNDAINYAANIIF
                     GPSVGRLLSFIVILSVLGVANGLLLGTMRLPQAFAERGWIKSERMANINLKYQMSLPA
                     SLTVMAVAIFWLFVHFMVTKFNLLPGSDISEIAVVFNNTSLIILYVLVLSLYLKKDIK
                     NKFTGLVSPILAILGGLILFIGSLLSNFFTVLIFQCFCLLFCLICHYIYQKNNPKTHE
                     "
     gene            22528..23877
                     /gene="trkA"
                     /locus_tag="VBE22_000027"
     CDS             22528..23877
                     /gene="trkA"
                     /locus_tag="VBE22_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEO7201509.1"
                     /translation="MRIIVVGGGKVGTALCRSLVAEKHDVVLIEKKENVLKRVTKQHD
                     IMGIVGNGANYKILEQAEVKNCDIFIAITDRDEVNMISAVLAKKMGAKETVVRMRNPE
                     YSNPYFKDKNFLGFSSVVNPELLAAQYIANTIEFPNATSVEHFANGRVMLMEFKILEG
                     NKLCHTSMSQIRKKFGNIVICAIERDGKLIIPDGDATIQVKDKIFVTGNRIEMILFHN
                     YVKNKVVKNLMVIGAGRIAYYLLNILKNTNTHVKLVELNQEQAEYFSQEFPNVPVVHG
                     DGTAKNILLEESVTSFDAVATLTGVDEENIITSMFLESIGIPKNITKVNRTSLLEIID
                     DKQLSSIITPKRIAVDHVMHFVRGRVNAQDSNLEAMHHIANDRIETLQFEIKETSKLA
                     NRSLASLKLKQNILIAAIIRNNKTIFPTGEDVLTVGDRIVVITLLKNITRTSDMLAR"
     gene            23882..25321
                     /locus_tag="VBE22_000028"
     CDS             23882..25321
                     /locus_tag="VBE22_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO7201510.1"
                     /translation="MNKSMIRFLLSKLLLIEAALLAIPLTVGLIYREPQSVMMSIVIT
                     MIILIILGLLGSLFKPKNYHIYTKEGMLIVALCWILWSFFGALPFVISGQIPNIIDAF
                     FEVSSGFTTTGATILDDVSVLSPALLFWRSFTHLIGGMGVLVFALAIMENSKNSHLEV
                     MRAEVPGPVFGKVVSKLKKTAQILYLLYLLMFAVFAVILYFAGMPFFDSIIIAMGTAG
                     TGGFAVYNDSIAHYNSPLITNLVSIGMLIFGVNFNLYYLLLLRKIKAFFGDEELKNYL
                     RIVAIATFMIALNVIGMYDNFRQGLEHIFFEVSAIITTTGFGVTDITRWPLFSQVILL
                     FLMFIGGSAGSTAGGFKVMRSLSLAKIARNQVLSTLYPNRVMSLHINKSVLDKNTQHG
                     VLKYLTIYLAIFMALVLVLTLDTNDFLVVVSAAASCFNNIGPLLGSNETFSFFSPFSK
                     LLLSFAMIAGRLEIYPVLLMFIPKTWSKL"
     gene            complement(25345..25596)
                     /gene="yidD"
                     /locus_tag="VBE22_000029"
     CDS             complement(25345..25596)
                     /gene="yidD"
                     /locus_tag="VBE22_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEO7201511.1"
                     /translation="MLKSFLIFLVRFYQKNISPAFPASCRYRPTCSTYMIEAIQKHGL
                     KGVLMGIARILRCHPLAHGGNDPVPDHFSLRRNKTDISD"
     gene            complement(25596..26318)
                     /locus_tag="VBE22_000030"
     CDS             complement(25596..26318)
                     /locus_tag="VBE22_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO7201512.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGMVTINGQVVNELATKVKAG
                     DLVEIEGSPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVIDLLPQVKERIYPVGRLDW
                     DTTGLLILTNDGDFTDKMIHPRNEIDKVYLARVKGIATKENLRPLTRGVVIDGKKTKP
                     ARYNIIKVDHEKNRSVVELTIHEGRNHQVKKMFEQVGLLVDKLSRTQFGTLDLTGLRP
                     GEARRLNKKEISQLHNAAINKS"
     gene            complement(26308..26892)
                     /locus_tag="VBE22_000031"
     CDS             complement(26308..26892)
                     /locus_tag="VBE22_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEO7201513.1"
                     /translation="MTYLGSIEALLFVAGEDGLSLRQMAELLSLTPSALIQQLEKLAK
                     RYEEDDDSSLLLLETAQTYKLVTKDSYMTLLRDYAKAPINQSLSRASLEVLSIIAYKQ
                     PITRIEIDDIRGVNSSGAITRLIAFGLIKEAGKKEVLGRPNLYETTNYFLDYMGINQL
                     DDLIDASSIELVDEEVSLFSMDSINTEDKENNEN"
     gene            complement(26889..27596)
                     /locus_tag="VBE22_000032"
     CDS             complement(26889..27596)
                     /locus_tag="VBE22_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEO7201514.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYEVDIYDVPIVEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMLIKSRNLLPKVVESTPIEDDPEMELLSQLEEYRRFKVL
                     SEELANQHQERAKYFSKPKQEVIFEDAILLHDKSVMDLFLTFSQMMSQKQKELSNSQT
                     VIEKEDYRIEDMMIVIERHFNLKKKTTLQEVFADCQTKSEMITLFLAMLELIKLHQIT
                     VEQDSNFSQVILRKEEK"
     gene            complement(27596..28336)
                     /gene="xerD"
                     /locus_tag="VBE22_000033"
     CDS             complement(27596..28336)
                     /gene="xerD"
                     /locus_tag="VBE22_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEO7201515.1"
                     /translation="MINDINNFIESKKLSLNSRKSYHYDLKQFYKIIGGHVNSEKLAL
                     YQQSLSEFKLTARKRKLSAVNQFLFFLYNRGTLKEFYRLQETEKITLTQTKSQIMDLS
                     NFYQDTDYPSGRLIALLILSLGLTPAEIANLKKADFDTTFNILSIEKSQMKRILKLPE
                     NLLPFLLESLEEDGDLVFEHNGKPYSRQWFFNQLTDFLNEKNEQQLTAQLLREQFILK
                     QKENGKTMTELSRLLGLKTPITLERYYR"
     gene            complement(28326..28799)
                     /locus_tag="VBE22_000034"
     CDS             complement(28326..28799)
                     /locus_tag="VBE22_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO7201516.1"
                     /translation="MIAKEFESFLLSHLDHYLIPAEDVAIFVDTHNADHVMLLLASNG
                     FSRVPVITKEKKYVGTISISDIMAYQSKGQLTDWEMAQTDIVEMVNTKIEPINEAATL
                     TAIMHKIVDYPFLPVISDQNDFRGIITRKSILKAINSLLHDFTDEYTITPKNNDK"
     gene            complement(28796..29317)
                     /locus_tag="VBE22_000035"
     CDS             complement(28796..29317)
                     /locus_tag="VBE22_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO7201517.1"
                     /translation="MAIRKLVVMSDSHGDRDIVKDIKNHYLGKVDAIFHNGDSELPSS
                     DPIWEGIHVVTGNCDYDSGYPEVLVTKIDNTVIVQTHGHLHQINFTWDKLDLLAQQED
                     ADICLYGHLHRADAWKNGKTIFINPGSVLQPRGPINEKLYAVVTITDSKVLVEYYTRQ
                     HQPYPNLTKEFSR"
     gene            complement(29299..30273)
                     /locus_tag="VBE22_000036"
     CDS             complement(29299..30273)
                     /locus_tag="VBE22_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEO7201518.1"
                     /translation="MTKTIFESKTEGNWFLGSFQAFNYFTCFGNDESYEAIQDVFHRL
                     LSTLKVEGLQLHVVQMTSDFQLLAFLVDMINQEYSRHIKVTQHKGAILVSEDDQLFLV
                     HLPKEGTSLEKFFDLKNDNNFGDTILIATHNEGKTKEFRELFGKLGLKVENLNDYPDL
                     PEVEETGMTFEENARLKAETISKLTGKMVISDDSGLKVDALGGLPGVWSARFSGPDAT
                     DARNNAKLLHELAMVFDKERRSAQFHTTLVVSAPNKESLVVEAEWPGYIGTEPKGENG
                     FGYDPLFIVGEGSRTAAELSAQEKNNLSHRGQAVRKLMEVFPKWQLEN"
     gene            complement(30270..31064)
                     /gene="racE"
                     /locus_tag="VBE22_000037"
     CDS             complement(30270..31064)
                     /gene="racE"
                     /locus_tag="VBE22_000037"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEO7201519.1"
                     /translation="MDSRPIGFLDSGVGGLTVVKEMFRQLPEEEVIFIGDQARAPYGP
                     RPAQQIREFTWQMVNFLLTKNVKMIVIACNTATAVAWQEIKEKLDIPVLGVILPGASA
                     AIKSTNSGKVGIIGTPMTVKSDAYRQKIQALSPNTAVVSLACPKFVPIVESNQMSSSL
                     AKKVVYETLSPLVGKLDTLILGCTHYPLLRPIIQNVMGAEVKLIDSGAETVRDISVLL
                     NYFEINHNWQNKHGGHHFYTTASPKGFKEIAEQWLSQEINVERIVL"
     gene            complement(31239..31478)
                     /locus_tag="VBE22_000038"
     CDS             complement(31239..31478)
                     /locus_tag="VBE22_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEO7201520.1"
                     /translation="MSTTIWILLIIVALFGGLVGGIFIARKQIEKEIGEHPRLTPDAI
                     REMMSQMGQKPSEAKVQQTYRNIVKHAKTAIKTKK"
     gene            complement(31636..32178)
                     /locus_tag="VBE22_000039"
     CDS             complement(31636..32178)
                     /locus_tag="VBE22_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201521.1"
                     /translation="MSKKTTQMVSYTSILVAFAIMIPIIMPAKIIIGPASFTLASHVP
                     LFLSIFISVPVAILVALGTGLGFLLAGFPIVIVLRALSHIGFALIAAFLIKSKPSLLM
                     SKWQTLLFAVAINIIHGLLEFITVYIITMTSNSSSTYLWSLFSLIGLGSLLHGLVDFY
                     IALFIWKWMTQKLGKTLRIR"
     gene            complement(32240..32761)
                     /locus_tag="VBE22_000040"
     CDS             complement(32240..32761)
                     /locus_tag="VBE22_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEO7201522.1"
                     /translation="MKAQERRENILTTLKGTKEAISASTLAKIFSVSRQVIVGDIALL
                     RAQQCDIISTPKGYLMSSALSTHQFTARLVCQHGIEQTEEELKIILRYQGIIMNVEVE
                     HPIYGMLTAPLNIQSQKDIDNFTAKLKVSNAELLSSLTDGLHTHMISCQDQSVFDQIC
                     EALKKAGILYIDK"
     gene            complement(32829..33518)
                     /locus_tag="VBE22_000041"
     CDS             complement(32829..33518)
                     /locus_tag="VBE22_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEO7201523.1"
                     /translation="MNDNVIYTQSDSGLNQFFAKIYGLVGIGVGLSAAVSAIMLYMFP
                     QNMIAIMQKMPGLYFGAIILELVLVFVASGAARRNTPAALPLFLIYSALNGFTLSFII
                     ARYTQTTVLQAFITSAAVFFAMALIGAKTKKDLSGMRKALMAALIGILIASLVNLFIG
                     SGGMSYIISIVCVIIFSGLIAYDNQMIKYVYNSQGGQVADGWAVSMALSLYLDFINLF
                     LNILRLFARND"
     gene            complement(33541..34044)
                     /locus_tag="VBE22_000042"
     CDS             complement(33541..34044)
                     /locus_tag="VBE22_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO7201524.1"
                     /translation="MKKYQDDKDFMDLVGHLIDHPRFQKLEAIVQHHHSTRLEHSINV
                     SYTSYKIAKKFGWDASSTARGGLLHDFFYYDWRVTKFNKSHAWVHPRIAVRNARKLTD
                     LNAREEDIILKHMWGATIAPPRYKESYIVTMVDKYWAVREASRPLKRIFKKPIRFSRK
                     FLGSHNH"
     gene            complement(34082..34825)
                     /locus_tag="VBE22_000043"
     CDS             complement(34082..34825)
                     /locus_tag="VBE22_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEO7201525.1"
                     /translation="MTTIITSKSNNLIKKTKKLLQKKYRKSSYLIEGWHLFEEAEKYG
                     AQFLNIFVTETAIDRLRKPEEAIVVTDDVLKELTDSQTPQGIVAEIAFQETRWTDIKK
                     GRFLVLEDVQDPGNLGTMVRTADAANFDAVFLSQKSADLYNQKTLRSMQGSHFHLPVF
                     RVEIEQFVNFCKAEGITMIATTLSEQSVNYKNLPKYDHFALIMGNEGQGISKTMTEEA
                     DVLAHIEMPGQAESLNVAVAAGVVIFSLI"
     gene            34857..35135
                     /locus_tag="VBE22_000044"
     CDS             34857..35135
                     /locus_tag="VBE22_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEO7201526.1"
                     /translation="MKKVHLIVSGRVQGVGFRYATYSLALEIGDIYGRVWNNDDGTVE
                     ILAQSTDSNKMTQFIQKIRKGPSKWSKVTYVDIKLDNFDDFNDFKMRN"
     gene            35220..36152
                     /gene="yidC"
                     /locus_tag="VBE22_000045"
     CDS             35220..36152
                     /gene="yidC"
                     /locus_tag="VBE22_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO7201527.1"
                     /translation="MKKTLKRILFSSLSLSILLLLTGCVSVDKAGKPYGVIWNTLGVP
                     MANLITYFAQHQGLGFGVAIIIVTIIVRVIILPLGLYQSWKASYQAEKMAYFKPLFEP
                     INERLRNAKTQEEKLAAQTELMTAQRENGLSMFGGIGCLPLLIQMPFFSAIFFAARYT
                     PGVSSATFLGLNLGQKSLTLTVIIAILYFVQSWLSMQGVPDEQRQQMKTMMYVMPIAM
                     VFMSISLPASVALYWFIGGIFSIIQQLVTTYVLKPKLRRKVEEEYTKNPPKAYKSNNA
                     RKDVTSSTKTTESNQAIITSKKTNRNAGKQKRRG"
     gene            complement(36351..37016)
                     /locus_tag="VBE22_000046"
     CDS             complement(36351..37016)
                     /locus_tag="VBE22_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7201528.1"
                     /translation="MINWDAIFNLELAVKAFPSVIQGLPYTIGLSLVGFILGAIVGFF
                     VALMKMSHLRLLRYLANIHISLMRGIPLMVLLFLIYFGLPFIGIQLDAVTASIVGFTM
                     MSSAYISEIIRAALLAVDHGQWEAARALGLKTPTIYRGIIIPQATRIALPSLSNVLLD
                     MVKGSSLTAMITVPDIFNNAKIVGGTYSDYMTAYILVALIYWVICTLYAIIQDWWEKR
                     LLY"
     gene            complement(37040..37897)
                     /locus_tag="VBE22_000047"
     CDS             complement(37040..37897)
                     /locus_tag="VBE22_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7201529.1"
                     /translation="MKKTILLGLVGLSAMTLAACSNGQSSKETTWDNIKKDGVLKVAT
                     PATLYPTSYYDDHKKLTGYEIDMMKAIAKKLKIKVKFVEVGVAESFTSVDSGKVDVAV
                     NNFDTTPERLKKYNFSQPYKYSVGGMIVRADGSSKITAKDLSDWKGKKAGGGAGTQYM
                     KIAKQQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVIAVKYVTKQYPDIKVKM
                     GDVKYNPTEQGIVMSKKDKSLKTKIDAAIKDMKKDGSLKKISEKYYAGQDLTKEPAGT
                     DKIPVIKVK"
     gene            complement(38058..39518)
                     /locus_tag="VBE22_000048"
     CDS             complement(38058..39518)
                     /locus_tag="VBE22_000048"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO7201530.1"
                     /translation="MVFKDATAMAQAIKQHKVSSQELVEQAIYKIEEQNVSVNAVVSK
                     QYNEARQAAKYANASNAPFAGVPILLKDLGQNQKGQLSTSGSQLFKHYYAKQTDYLVQ
                     SFEKLGFIILGRTNTPEFGFKNISDSQLHGNVNLPFDHSRNAGGSSGGAAAAVSSGMV
                     PIAGASDGGGSIRIPASFNGLIGLKPSRGRIPVGPSSYRGWQGASSHFALTKSVRDTK
                     RLLYYLQSYQVESPFPLKKLSKESLFEFSVSKPLKIAVLMDSPLKTKVSSEAKAAIKE
                     AADFLSQKGNHLELVEQPLDGIHSMKTYCMMNSVETAAMFDGIEKSLGRSMEFSDMEL
                     MTWAMYQSGQRVLAKDYSKLLDSWDQFAATMARFHENYDLILTAATNQPAPFHGQFDL
                     DETLQKQLRHMREFSVSEQQDLIWKMFEDSMAWTPFTHQPNLTGQPSLAIPTHLTKEG
                     LPLGVQLTAAKGREDLLLAVAELFEKEKQFKGPVCH"
     gene            complement(39622..40104)
                     /gene="greA"
                     /locus_tag="VBE22_000049"
     CDS             complement(39622..40104)
                     /gene="greA"
                     /locus_tag="VBE22_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEO7201531.1"
                     /translation="MAEKTYPMTQVEKDQLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQIQILETKIRYAEIIDSDAVAKDEVAIGKTVLVQEVGTND
                     KDTYHIVGAAGADIFSGKISNESPIAHALIGKKTGDLATIESPAGSYQVEIISVEKTN
                     "
     gene            complement(40177..41979)
                     /gene="mltG"
                     /locus_tag="VBE22_000050"
     CDS             complement(40177..41979)
                     /gene="mltG"
                     /locus_tag="VBE22_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO7201532.1"
                     /translation="MTEFNDDQHSNHDQKSFKEQILAELEEANRLRKLREEELYQKEQ
                     EAKEAARRTAQLMADYEAQRLKDEQEARAKALETKQRLEEQEKARIEAKLLAEAAREE
                     ERRQAEQALASQEEQVINQGMEPSRELDSGSKSSEFRTTENVPDIDLKADKTDVATAV
                     PNQETEEIFLVRATDIPTEGENVKLGETSELEPVAKEPIRVEDLSKEEEDIALSAKNK
                     HNKRERRQKADNVAKRIARILISIIILVLLLTAFVGYRFVDSAIKPVDSNSNKFVQVE
                     IPIGSGNKLIGQILEKAGVIKSATVFNYYSKFKNYSNFQSGYYNLKKSMTLDQIAAEL
                     EKGGTAEPTKPALGKILITEGYTIKQIAKAIESNKIDTKTTSTPYKADDFLKLVQDET
                     FIKKMVAKYPNLLGSLPDKSKAIYQLEGYLFPATYNYYKDTTLEGLVEDMISTMNTKM
                     APYYNTIKAKNMSVNDVLTLSSLVEKEGSTDEDRRKIASVFYNRLSAGQALQSNIAIL
                     YAMGKLGDKTSLAEDAQINTSIKSPYNIYTNTGLMPGPVDSPSISAIEATIKPASTDY
                     LYFVADVKTGNVYYAKDFETHKANVEKYINSQIN"
     gene            complement(42066..42569)
                     /locus_tag="VBE22_000051"
     CDS             complement(42066..42569)
                     /locus_tag="VBE22_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7201533.1"
                     /translation="MIIRNGCLEDLQQVISIERINFSEAEAASKKAMQERLMIMTDTF
                     LVAEINGRLAGYIEGPVIKGRYLTDDLFHKVSEIPVREGGFIGITSLSIHPDFKGQGI
                     GTALLAAMKDLVVSQERDGISLTCHDDLISFYEMNGFKDEGESDSQHGGSLWYNMIWN
                     NPTRSIC"
     gene            complement(42655..43986)
                     /locus_tag="VBE22_000052"
     CDS             complement(42655..43986)
                     /locus_tag="VBE22_000052"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEO7201534.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHNVQGSDVDKYYFTQRGL
                     EQAGVTILPFSPNNISEDLEIIAGNAFRPDNNEELAYVIEKGYHFKRYHEFLGDFMRQ
                     FTSLGVAGAHGKTSTTGLLAHVLKNITDTSFLIGDGTGRGSANANYFVFEADEYERHF
                     MPYHPEYSIITNIDFDHPDYFTGLEDVFNAFNDYAKQVQKGLFIYGEDSKLHEITSKA
                     PIYYYGFEDSNDFIAKDITRTVNGSDFKVFYNQEEIGQFHVPAYGKHNILNATAVIAN
                     LYIMGIDMALVAEHLKTFSGVKRRFTEKIIDDTVIIDDFAHHPTEIIATLDAARQKYP
                     SKEIVAIFQPHTFTRTIALLDDFAHALSQADSVYLAQIYGSAREVDNGEVKVEDLAAK
                     IVKHSDLVTVENVSPLLNHDNAVYVFMGAGDIQLYERSFEELLANLTKNTQ"
     gene            complement(43996..44613)
                     /locus_tag="VBE22_000053"
     CDS             complement(43996..44613)
                     /locus_tag="VBE22_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201535.1"
                     /translation="MNRGSRRFPLIADDEPVMSPLVKMNLYDNEDLINNIRGFYQEKT
                     YQSMVKSNYEHEEISHPKVIENDPVPPQSFVKKATELSKSRQEAKRSVREKRQAYYAK
                     QEFKAPSKEAFQQQLKATVPKKQTQRKVTELSHLSDRLQQESYILAEIPIIFQEPDNT
                     PNPKTKKNNFDFLKRSQVYNKQDNQFHKERAKAQELNLTRFKDIN"
     gene            complement(44778..47876)
                     /locus_tag="VBE22_000054"
     CDS             complement(44778..47876)
                     /locus_tag="VBE22_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7201536.1"
                     /translation="MSRMIPGRIRNQGIELYEQGLVSLISQEGNLLKAKVGDCQIEYS
                     LVTEETKCSCDFFARKGYCQHLAALEHFLKNDPEGKAILSKVQVQQESQQETKKKTSF
                     GSVFLDSLIINEDDTIKYQLSAQGEQNPYANDIWWTLKIRRLPDDRSYVIRDIKAFLN
                     TVRKEAYYQIGKQYFETLSLIQFDETSQELIEFLWRLIPSHSSKIDLEFILPNQGRHL
                     SLTRGFFEEGVTLMNALENFSFESDFHQFNHLYFKELEGEDHLYQFKVIVHRQSIELE
                     IKEKDLKPLFANSYLFYRDTFYHLNLKQEKMVTAIRSLPIEGDLAKHIHFDLDDQDKL
                     AAHLLDFKEIGLVDAPRSFSIHDFKVNFEFDINSQNEILLQMVFDYGNDLTVHNRQEL
                     EQLTFASHFKHEEKIFKLLERYGFAPHFSTSHPAYSAQELYDFYTYMLPQFKKMGTVS
                     LSAKLESYRLIERPQIDIEAKGSLLDISFDFSDLLENDVDQALVALFDNNPYFVNKSG
                     QLVIFDEETKKVSATLQGLRARRAKNGHIELDNIAAFQLSELFANQDNVSFSQHFYQL
                     IEDLRHPEKFKIPGLSVSASLRDYQLTGVRWLSMLDHYGFAGILADDMGLGKTLQTIS
                     FLSTKLTRDSRVLILSPSSLIYNWQDEFHKFAPDVDVAVAYGSKIRRDEIIAERHQVI
                     ITSYSSFRQDFETYSEGNYDYLILDEAQVMKNAQTKIAHSLRSFEVKNCFALSGTPIE
                     NKLLEIWSIFQIILPGLLPGKKEFLKLNPKQVARYIKPFVMRRRKEEVLPELPDLIEM
                     NYPNEMTDSQKVIYLAQLRQIQESIQHSSDADLNRRKIEILSGITRLRQICDTPRLFM
                     DYDGESGKLESLHQLLTQIKENGHRALIFSQFRGMLDIAEREMVAMGLTTYKITGSTP
                     ANERHEMTRAFNAGSKDAFLISLKAGGVGLNLTGADTVVLIDLWWNPAVEMQAISRAH
                     RLGQKENVEVYRLITRGTIEEKILEMQETKKHLVTTVLDGNETHASMSVDDIREILGV
                     SK"
     gene            complement(48032..49342)
                     /gene="der"
                     /locus_tag="VBE22_000055"
     CDS             complement(48032..49342)
                     /gene="der"
                     /locus_tag="VBE22_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEO7201537.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYT
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQADIAMTEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPLSSVHGIGTGDILDAIV
                     ENLPVEEENENPDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTNFV
                     DSQGQEYTMIDTAGMRKSGKVYENTEKYSVMRSMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGIIIVVNKWDTIEKDNHTVSQWEADIRDNFQFLSYAPIIFVSAETKQ
                     RLHKLPDMIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYLRFLENQIREAFVFEGTPINLIARKRK"
     gene            complement(49390..50292)
                     /gene="dnaI"
                     /locus_tag="VBE22_000056"
     CDS             complement(49390..50292)
                     /gene="dnaI"
                     /locus_tag="VBE22_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEO7201538.1"
                     /translation="MKSVGQALENQGRVPRNTNDELIQMILSDAQVAEFIKTHQLSQR
                     EINISMSKFNQFLIERQKFKNKDSQYIAKGYEPILVMNEGYADVSYLETRELIEAQKK
                     QAISDRINLVNLPKSYRNIRMTDFDINNESRMKAMSQLLDFVETYPSYNHKGLYLYGD
                     MGVGKSYLMAAMARELSERKGVSTTLLHFPSFAIDVKNAISSGTVKDEIDAVKSVPIL
                     ILDDIGAEQATSWVRDEILQVILQHRMLEELPTFFTSNYSFNDLERKWANIKGSDETW
                     QAKRVMERVRYLAIEFHLEGPNRR"
     gene            complement(50289..51464)
                     /locus_tag="VBE22_000057"
     CDS             complement(50289..51464)
                     /locus_tag="VBE22_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO7201539.1"
                     /translation="MSPIDEFTYIKQNKIVYDSNSLIQLYFPIMGSDAMALYDYFVHF
                     FDDGIRRHKFSEVLNHLQYGMPRFQDALVMLTALDLLTVYQATGTYLVKLNQAMSNEL
                     FLSNPIYRRLLEKRIGEVAVAELDMKIPKNARDISKKFTDVFSDLGQPKQEVNRSKNV
                     FDLESFKRLMMRDGLRFNNEKDDVLGIYSVSELYHLNWYDTYQLAKQTAINGMIAPQR
                     MKVQQNEGQHIKDNQSFTNNEKVILRESKNDSALVFLEKIKRSRKAVTTSGEKTLLED
                     LAKMNFLDEVINVMVLYTLNKTKSANLNKAYIMKVANDFAFQNVMTAEDAVLKIRDFS
                     DQKVRTKTETKKKQSNVPEWSNPDYKDEVSPEKEIELEQFKTDALKRLERLGKDGES"
     gene            complement(51464..51943)
                     /gene="nrdR"
                     /locus_tag="VBE22_000058"
     CDS             complement(51464..51943)
                     /gene="nrdR"
                     /locus_tag="VBE22_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEO7201540.1"
                     /translation="MRCPKCGYNKSSVVDSRQAEEGTTIRRRRECEKCGNRFTTFERL
                     EELPLLVIKKDGTREQFSRDKILNGIIQSAQKRPVSSEDIENCILRIERKIRSEYEDE
                     VSSITIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITKRVRSKKSGSV"
     gene            complement(52092..53597)
                     /locus_tag="VBE22_000059"
     CDS             complement(52092..53597)
                     /locus_tag="VBE22_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEO7201541.1"
                     /translation="MKNKKDQFIGVKQPLSKKLSQLVFILFFSLFTVFSVLVYTSATR
                     YVLHREKINVGRSLEKTRVRLSQANSSLTSDDILEILYNQVFADDIYPHKRQNGIVRT
                     GESIDSILYVNQEMTLYDVNRKPVFSTLRTGMPTIGKSMGKVIISKVADMEGFVGTKA
                     IYSQKTGQLLGYVQIFYNLGRYYSMRQNIIVFLIMMEVLGTVLALVVINSATKRIVRP
                     VKNLHDLMHQISENPSNLEIRSKVRSEDEIGELSRIFDGMLDQLEDYTRRQSQFISDV
                     SHELRTPVAVVKGHIGLLQRWGKDDPEILEESLAAAYHEADRMSLMINDMLNMIRVQG
                     SLELHQDEVTDLSSSISVVIENFRILREDFQFIFENNISDIVWGKIYKIHFEQALMIL
                     IDNAIKYSPSYKEVSVVLSVDNDFATVVVKDKGEGISDEDIEFIFDRFYRTDKSRNRE
                     STQAGLGIGLSVFKQIMDAYHLKVDIKSELNQGTEFIVRIPIKKFEETKEI"
     gene            complement(53587..54276)
                     /locus_tag="VBE22_000060"
     CDS             complement(53587..54276)
                     /locus_tag="VBE22_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7201542.1"
                     /translation="MGKKILIIEDEKNLARFVSLELLHEGYDVVVETNGREGLDTALE
                     KDFDLILLDLMLPEMDGFEITRRLQAEKTTYIMMMTARDSVMDIVAGLDRGADDYIVK
                     PFAIEELLARVRAIFRRQEIETKTKEKGDSGSFRDLSLNTHNRSAMRGDEEISLTKRE
                     FDLLNVLMTNMNRVMTREELLEHVWKYDVAAETNVVDVYIRYLRGKIDIPGRESYIQT
                     VRGMGYVIREK"
     gene            complement(54511..55044)
                     /locus_tag="VBE22_000061"
     CDS             complement(54511..55044)
                     /locus_tag="VBE22_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEO7201543.1"
                     /translation="MLLTEIKKSPEGLYFDKKIDIKESLMERHSEIMDISDIQVSGHV
                     VYEDGLYLLDYNMAYDITLPSSRSMKPVVLSEKQTINEVFIEAENVSTKKELVDQDLV
                     LILEEDDINLEESVIDNILLNIPLRVLAADEVGVEADLSGKNWSLMTEKQYEEKQAKE
                     KEKSNPFAALEGMFDSD"
     gene            complement(55149..56039)
                     /gene="htpX"
                     /locus_tag="VBE22_000062"
     CDS             complement(55149..56039)
                     /gene="htpX"
                     /locus_tag="VBE22_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEO7201544.1"
                     /translation="MLYQQIASNKRKTVVLLIVFFCLLAAIGAAVGYLVLGSYQFGLV
                     LALIIGVIYAVSMIFQSTNVVMSMNNAREVTEDEAPNYFHIVEDMAMVAQIPMPRVFI
                     VEDDSLNAFATGSKPENAAVAATTGLLAVMNREELEGVIGHEVSHIRNYDIRISTIAV
                     ALASAVTLISSIGSRMLFYGGGRRRDDDREDGGNILVLIFSILSLILAPLAASLVQLA
                     ISRQREYLADASSVELTRNPQGMISALEKLDRSEPMGHPVDDASAALYINDPTKKEGL
                     KSLFYTHPPIADRIERLRHM"
     gene            complement(56060..56614)
                     /locus_tag="VBE22_000063"
     CDS             complement(56060..56614)
                     /locus_tag="VBE22_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEO7201545.1"
                     /translation="MGTMILIAIIALFVIWLIVAYNSLVRSRMHTKESWSQIDVQLKR
                     RNDLIPNLIETVKGYAAYEGKTLEKIAELRAQVAKANTPAEAMTASNELTRQLSSILA
                     VAENYPDLKANNSFVKLQEELTNTENKISYSRQLYNTTTSNYNVKLETFPSNIVGKLF
                     GFKPSQFLETPEEEKEVPKVSFDF"
     gene            56742..57455
                     /gene="rsmG"
                     /locus_tag="VBE22_000064"
     CDS             56742..57455
                     /gene="rsmG"
                     /locus_tag="VBE22_000064"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEO7201546.1"
                     /translation="MTPQAFYQVLIEHGITLTDKQKKQFETYFRLLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGYIDNSPLSILDIGAGAGFPSIPMKILYPEIDITIIDS
                     LNKRINFLNILANELELSGVHFFHGRAEDFGQDKVFRAKFDIVTARAVARMQVLAELT
                     IPFLKVNGRLIALKAAAAEEELISAEKALKTLFSQVTVNKNYKLPNGDDRNITIVSKK
                     KETPNKYPRKAGTPNKKPL"
     gene            57470..58849
                     /locus_tag="VBE22_000065"
     CDS             57470..58849
                     /locus_tag="VBE22_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO7201547.1"
                     /translation="MKHFFDYKTMSVARKLSISFIAVILLGSILLSLPIFQYANAPKT
                     HYIDHLFTTVSMVCVTGLSVFPISKVYNGWGQIVAILLMQTGGLGLVTLMSLSYYTLR
                     RKMSLNDQTLLQSAITYNSSTDLKKYLYMIFKVTLTLEVLAASILAIDFIPRFGLGHG
                     IFNSIFLAVSAFCNAGFDNLGATSLAQFKLNPLVNIIVCFLIISGGLGFAVWKDLIEA
                     TIQTSHKGPKLIKTFPKRLSNHSKLVLKTTTIILLTGTLLSWLLEFGNFRTIANLSLP
                     KQLMVSFFQTVTMRTAGFSTIDYTQTDFATNLVYIIQMLIGGAPGGTAGGFKVTVIAI
                     LLLLFKAELSGQSQVTFHYRTIPSSIIKQTLSILTFFFIILISGYLLLLELNPHIDPF
                     SLFFEASSALATVGVTMNTTNQLSLGGRIVIMFLMFIGRVGPITVLLSILQKKEKEIH
                     YAETEIILG"
     gene            58862..59533
                     /locus_tag="VBE22_000066"
     CDS             58862..59533
                     /locus_tag="VBE22_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEO7201548.1"
                     /translation="MKTKIIGVLGLGIFGQTLAQELSNFEQDVIAIDSNPENVQAVAE
                     VVTKAAIGDITDLAFLKHIGISDCDTVIIATGNSLESSVLAVMHCKKLGVPQVIAKAR
                     NLVYEEVLYEIGADLVISPERESGQNVAANLMRNKITDVFQIESDISVIEFKIPKSWV
                     GKTVEQLNIRHKFDLNLIGIRKAKNKPVDTEVPINSPLEEGIILVAIANSDAFQRYDY
                     LGYFN"
     gene            complement(59568..60398)
                     /locus_tag="VBE22_000067"
     CDS             complement(59568..60398)
                     /locus_tag="VBE22_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO7201549.1"
                     /translation="MANQLIGYQEGYGFLYRLSGASKLLFFILVSVACMTTYDTRFLI
                     LVSLGSLVLFRLAKIKWQQVSFVMTLVVVFAVLNIIMVYLFAPHYGDKIYGSSSLLLK
                     GIGPYDVTSQELFYLFNLILKYFCTVPLALLFLMTTNPSQFASSLNQLGLSYKIAYAV
                     SLTLRYIPDVQEEFYTIRRAQEARGIELSKKSNLVARIKGNLQIVTPLIFSSLERIDT
                     VATAMELRRFGKNKRRTWYSKQSLEKSDIVLIILALASLFVSLYLIHLNHGRFYNPWQ
                     "
     gene            complement(60391..62067)
                     /locus_tag="VBE22_000068"
     CDS             complement(60391..62067)
                     /locus_tag="VBE22_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7201550.1"
                     /translation="MKDFIEWKDFTFQYDVQSEPTLKGINLSIPKGEKVLILGPSGSG
                     KSTLGHCLNGIIPNTHKGQYSGIFTINHKNAFDLSIYDKSHLVSTVLQDPDGQFIGLT
                     VVEDIAFALENDVVAQEEMASIVEMWAKRLEIAPLLSKRPQDLSGGQKQRVSLAGVLV
                     DDSPILLFDEPLANLDPQSGQDIMALVDRIHQEQDATTIIIEHRLEDVLYERVDRVVL
                     FSDGQIIYNGEPDQLLKTNFLSEYGIREPLYISALKNLGYDFEKQNTMTSIDDFDFSE
                     LLIPKMRALDLDKHTDKLLSVQHLSVSYDLENNTLDDVSFDLYKGQRLAIVGKNGAGK
                     STLAKALCQFIPNNATLIYNNEDVSQDSIKERAERIGYVLQNPNQMISQAMVFDEVAL
                     GLRLRGFSDNDIESRVYDILKVCGLYQFRNWPISALSFGQKKRVTIASILILNPEVII
                     LDEPTAGQDMKHYTEMMSFLDKLSCDGHSIVMITHDMQLMLEYTDRAIVIDNGLIAAD
                     DHPINILSNTELLKKTHLKKTSLFALADRLGISPQKLTQWYIDNQGGKNG"
     gene            complement(62084..62632)
                     /locus_tag="VBE22_000069"
     CDS             complement(62084..62632)
                     /locus_tag="VBE22_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEO7201551.1"
                     /translation="MNTNTIKKVVATGIGAALFIIIGMLVNIPTPIPNTNIQLQYAVL
                     ALFAVIYGPGVGFFTGFIGHALKDSIQYGSPWWTWVLVSGLLGLMIGFFAKKLAIQLS
                     GMTKKDLLLFNVVQVIANLIGWSVVAPYGDIFFYSEPASKVFAQGFLSSLVNSITIGV
                     GGTLLLLAYAKSRPQKGSLSKD"
     gene            complement(62801..64009)
                     /gene="sstT"
                     /locus_tag="VBE22_000070"
     CDS             complement(62801..64009)
                     /gene="sstT"
                     /locus_tag="VBE22_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEO7201552.1"
                     /translation="MKRFLKAWRKTSLIKKITIGVVIGLFLGILVPKASAIGLLGQLF
                     VGGLKAIAPLLVFTLVISALSQHREGGKTNMSTIIGLYITATFAAALIAVVVNYIFPL
                     TLILKTPAKTDLLPPKGISEVFQSLLLKIVDNPIHAITEANYMSILFWAVIFGLAMRS
                     SNQRTKDLMQTFADATSQVVKWIINLAPIGIMGLVFTSISENGIAILGDYGLLILVLV
                     GTMLFVALVVNPIIAFVMMRKNPYPLVLRCLKDSGITAFFTRSSAANIPVNMRLCEDL
                     GLDKDTYSVSIPLGAAINMAGAAITINILTLAAVNTLGITVDFPTAFLLSVVAAVSAC
                     GASGVTGGSLLLIPVACSLFGISNDVAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     VAEKSVFGKK"
     gene            64185..65552
                     /gene="brnQ"
                     /locus_tag="VBE22_000071"
     CDS             64185..65552
                     /gene="brnQ"
                     /locus_tag="VBE22_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO7201553.1"
                     /translation="MKLKNTYIIIGLMLFALFFGAGNLIYPAYLGIYSGSNWAWAIIG
                     FCLTGVTLPLLGVAAVAYSGATDVESFTKPVSKTYAIFFAVALYLSIGPFFAIPRTGA
                     TSFSVGIQPILGDSSLGKIIYGLIFFGISYFLAIRPSQIVDRIGKYLTPFLLTVISIL
                     IIASLIHPAAIVGTPHNASTNVSDAFKDLPFLAGLIQGYGTMDALASIAFAIIVIQAS
                     KQYGAITKKEITSMALKSGAIATFLLAFIYIFVGRIGATSQSLFKFANGSFLLHNTPI
                     DGGHVLSQSANFYLGIVGQAILGTAIFLACLTTATGLITACAEYFHKLLPKISHITWA
                     TIFTLIAITFYFGGLSEIIRWSLPVLYLLYPLTIVLIFLVFFDQKFESSCIVYQTSIA
                     ATAVAALYDALSKLGEMTGLFTIPSALTTFFTKVVPLGEYSMGWISFAICGVLVGLIL
                     KKVKA"
     gene            65666..66721
                     /locus_tag="VBE22_000072"
     CDS             65666..66721
                     /locus_tag="VBE22_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO7201554.1"
                     /translation="MRAATYLSANELSLTDKAKPQVIKPTDAVVRLVKTTICGTDLHI
                     LGGDVPACKPGTTLGHEGIGIVEEVGSAVNHFKVGDKVIISCVTACNTCYYCKHQLPS
                     HCEDGGWILGHLIEGTQAEYVHIPHADGSLYHAPEGVCDDALVMLSDILPTSYEIGVL
                     PSHIKPGDTVCIVGAGPIGLSALLTAQFYSPAKIIMVDLSQKRLEASKKFGATHTILS
                     TSTQEVKEEIDKITKGRGVDVVLECVGYPATFDICQNVVSIGGHIANVGVHGKPVEFN
                     LQDLWIKNITLNTGLVNANTTEMLLEVLETGKIDATQLVTHHFKLSEIEEAYKVFKAA
                     EENNTLKVIIENDITPA"
     gene            complement(66770..67462)
                     /locus_tag="VBE22_000073"
     CDS             complement(66770..67462)
                     /locus_tag="VBE22_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7201555.1"
                     /translation="MIEWIQTHLPNVYQMGWEGAYGWQTAIVQTLYMTFWSFLIGGLM
                     GLLGGLFLVLTSPRGVIANKLVFGVLDKVVSVFRALPFIILLALIAPVTRVIVGTTLG
                     SPAALVPLSLAVFPFFARQVQVVLAELDGGVIEAAQASGGTLWDIIVVYLREGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGSVAITKGYNYSRDDITLVATILILLLIFFIQF
                     LGDFLTRRLSHK"
     gene            complement(67459..68529)
                     /locus_tag="VBE22_000074"
     CDS             complement(67459..68529)
                     /locus_tag="VBE22_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEO7201556.1"
                     /translation="MSKEPIIKLDNIDVTFHQKKREINAVKDVTIHINQGDIYGIVGY
                     SGAGKSTLVRVINLLQEPSAGKITIDDQVIYDNKVTLTSTQLREQRREIGMIFQHFNL
                     MSQLTAEQNVAFALKHSGLSKEAKAAKVAKLLELVGLSDRAQNYPSQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQDLNKKLGLTIVLITHEMQIVKDIAN
                     RVAVMQNGKLIEEGSVLDIFSHPRESLTQDFIKIATGIDEAMLKIEQQEVVKNLPVGS
                     KLVQLKYAGHSTDEPLLNQIYKEFEVTANILYGNIEILDGIPVGEMVVILSGDEEKLR
                     QACQAITDSQVQLTLLKEGGKA"
     gene            complement(68522..69898)
                     /locus_tag="VBE22_000075"
     CDS             complement(68522..69898)
                     /locus_tag="VBE22_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO7201557.1"
                     /translation="MTLTKEENQIQKFLSDTIVQDDINILRDLIAIKSIFAQKVGLND
                     LASYLGEVFRKAGAEVIIDDSYSAPFIVANFKSSKVDAKRIIFYNHYDTVPADEVEQW
                     TEDPFTLSLRYGKMYGRGVDDDKGHITARLSAVKKYLSRHKGELPLDITFIVEGAEES
                     ASVGLDYYLEKYQEQLQGADLIVWEDGPKNPKGQLEIAGGNKGIVTFDLSVSSADVDI
                     HSSFGGVVDSSTWYLIQALNTLRDNKGHILVEGIYDKVIPPTKRELELVEKYSYRSAK
                     ALEDAYQLVLPSLADSHKTFLRKLYFEPSIAIEGITSGYQGEGVKTILPAYAKCKAEV
                     RLVPGLTPKGVLDSIQNHLKENGFNDIELTYTLGEMSYRSDMSAPSILKVVDLAEQFY
                     PEGVSLLPTSPGTGPMYLVHQALRAPIAAFGIGHANSRDHGVDENVSIADYYTHIELV
                     EALIESYE"
     gene            complement(70032..70856)
                     /locus_tag="VBE22_000076"
     CDS             complement(70032..70856)
                     /locus_tag="VBE22_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO7201558.1"
                     /translation="MKLKHIVLGLALTTLLGVTFSNQEVSASSTSSKVVKVGVMTFSD
                     TEKARWDKIEKLVGDKAKIKFTEFTDYTQPNQATANKDVDINAFQHYNFLENWNKENK
                     KNLIPLEKTYLAPIRIYSEKVKSLKKLKKGATIAIPNDATNGSRALYVLQSAGLIKLN
                     VSGKKVATVANITSNKKDINIQELDASQTPRALKDVDAAIINNTYIEQANLKPSDAIF
                     VEKSDKNSKQWINIIAGRKNWKKQKNAKAIQAILDAYHTDEVKKVIKDTSADIPQW"
     gene            complement(70997..71830)
                     /locus_tag="VBE22_000077"
     CDS             complement(70997..71830)
                     /locus_tag="VBE22_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7201559.1"
                     /translation="MTIKKVLSVTGIILVTVASLAACSSKSHTTKTGKKEVNFATVGT
                     TAPFSYVKDGKLTGFDIEVAKAVFKGSDNYKVTFKKTEWSSVFTGIDSGKFQMGGNNI
                     SYSSERSQKYLFSYPIGSTPSVLAVPKNSNIKAYNDITGHKTQVVQGTTTAKQLENFN
                     KKHQKNPVTLKYTNENITQILTNLSDGKADFKLFDGLTVNAIIKNQGLTNLKTIPLTM
                     RDQPYIYFIFGQDQKDLQKYVNNCLKQLRKDGTLSKIAKEYLGGDYVPNEKDLVTPKE
                     K"
     gene            complement(71932..72621)
                     /locus_tag="VBE22_000078"
     CDS             complement(71932..72621)
                     /locus_tag="VBE22_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEO7201560.1"
                     /translation="MLTKPIIGITGNEREMSDIPGYYYDSVSRHISEGVKNAGGLPVI
                     LPISEAESAKAYVEMIDKLIISGGQNVLPSYYGEEKIIESDDYSLARDIFEFALVDEA
                     LKQNKPIFAICRGMQLVNVALGGTLNQSIDNHYQEPYIGFAHYLNVEKGSFLEGFISG
                     DFRINSLHRQSVKLLAEGLIVSARDPRDGTVEAYESRTEQCIIGVQWHPELMLHQIEN
                     QTLFGYFVNET"
     gene            complement(72776..73492)
                     /locus_tag="VBE22_000079"
     CDS             complement(72776..73492)
                     /locus_tag="VBE22_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7201561.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKQGEPDPESN
                     SALKFVLDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADTVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            complement(73610..74596)
                     /gene="dhaQ"
                     /locus_tag="VBE22_000080"
     CDS             complement(73610..74596)
                     /gene="dhaQ"
                     /locus_tag="VBE22_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEO7201562.1"
                     /translation="MRKTEHHKMEYVFETIKAITKTNPKVEQIKDLPAIHYLGQSEQV
                     IPILSGGGSGHEPAHFGYVGEGMLSAAISGPIFVPPCASDILETIRFINRGKGVFVII
                     KNFEADLEEFSQAIEQARQEGIPIKYIVSHDDISVETSNFKIRHRGVAGTVLLHKIIG
                     QAALEGASLDELEQLGLSLTTSMATLGVASKSATILGQHQPVFDLEEGYISFGIGIHG
                     EPGYRTMPFVSMEHLANELVNKLKMKLRWQDGEAFILLINNLGGSSKMEELLFTNAVM
                     EFLALDDLQLPFIKTGHLITSLDMAGLSVTLCRVKDSRWIDYLKHKTDARAW"
     gene            complement(74606..>74706)
                     /locus_tag="VBE22_000081"
     CDS             complement(74606..>74706)
                     /locus_tag="VBE22_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO7201563.1"
                     /translation="YFIKYELINNSKEELFNRKVIEKIIRTSIENY"
BASE COUNT        23706 a        14706 c        11537 g        24757 t
ORIGIN      
        1 cgccatacat taggagtgcc tttctacttg taggttataa aattcatggc ctaacagcaa
       61 gttgacatat aaaagaggaa ttaatattaa tgataagctc cattcgccca gtttacttga
      121 aaaataagtc gagcaaacaa caccaaagat aaatcctcca ataataataa tcgtatttct
      181 agcaattaag aaaatatcag aatttttttc gtaaagtccc tttgttaata aataagcagc
      241 attcttgata ttaccagtca tcatcatatt agcataaggt gctcctctga gcgatttaaa
      301 ggtatctact tgtattgaag caaagaaggc tagtcctgcc acagtaaacg acgatggtaa
      361 gaaaggagtc aatataattg ttactatagc aacctgtgtc aacgcaaaac tagataatag
      421 gtaccagtgc agttgatgtt tattcgccca acgattcatg aaataagtaa acgattgacc
      481 taacatgaaa accattatag gtagcaaaaa agcaagcgct tgactataat gcttattact
      541 aagatgaata gcaaaagata acaaattccc tgtttgtaca cctgcaaaac gttttccttg
      601 tgttacataa gtaaaagcat tgatataacc tgaaataaaa cagagtgcaa ccgcacacct
      661 gagcccttca tatatttgat attgacggtg agacttttta gacatatttt cctctaactt
      721 tatggcaaac aaaaataaga ctgggcaatc ttgctcagca ctttttttct aatagattgg
      781 tttaaaatag tattaatatt agcactagct agcctaacag ttctaaataa attattaagc
      841 aatgataaat gtagttttag tatatcataa cctcagcatt gaagcaatct cactttatga
      901 aaggataaag aaaatctcat aagtcattta cccttaattt gaagtttaat tagttacttt
      961 taattttact accaaaaaac ttgcaccatt cttaacattt gttacaataa atacaaccta
     1021 ttaggaggtc agtacagatt atgcacagca ctatttataa atgtcatcat tgccgttatc
     1081 taatttataa aagttttaaa gatatctttc cttttgctct cattttttct tggattgaaa
     1141 tccttcaaaa actcgttata tcagatgatt cttttttagc tcaaacaaca aagtccagta
     1201 tttataaaaa cgagtggtta tatacggttt ccaatactct ttatgattta tctcatttac
     1261 taatctactt tatggctagt tattttgtgg ctagtttagt atggagaggc ttatctaacc
     1321 aaactaaaga aagtttttct gcaagtttac ttggctttgc cactgtttgg ttgcttctaa
     1381 cttcagttgt tccaggtaca gattatcact atcctagcca acccatttgg cttatgctgg
     1441 ctttgatttc gtatcttttt atctttttaa ctcgactgct tcaaaatcga catcatattt
     1501 tccagtatag tatgttagct atactactat taatctcttc tattttgtca catatcttat
     1561 atcgttatcc taatattgat ttagaatacc ttatacaaaa tcacttttct caatggctgg
     1621 gtgatgggcc acatcaatta attcctatta tgctttggtc tatcttagct atttttctaa
     1681 ccttatcagg tctttttgta ccagatattt tatttcgtcc ttactcctat ttttcagtag
     1741 ttagcgaaaa tttaaatgct gccttgagcc aacatacaga taaaatccct tatctgtata
     1801 ccttttatac tgttaaaaat agctttgcta tgtttggcgg tattggaatc ctattatcct
     1861 tatttttagc agttttatat gaaagtagaa aactacaatc gaagaattac tataaattga
     1921 cattactgac cttaactcct cttatttttg atcaaaattt accattttta gtaggtctac
     1981 ctgtcatttt acaaccaata ttattcattc cgatggtatt aacaactatc tttgcagagg
     2041 cttttggagc tcttatgctc tacctaaaat ttgtcgaccc tgcagtttat actgtcccaa
     2101 gtggtacacc tagtctacta tttggctttc ttgcttcaaa tggggattgg cgttaccttc
     2161 cagtcactgc aattattcta gttgttggct tctttattta tcgtccgttt gtcaaaatcg
     2221 ctttcgctaa ggaggagcaa tatgaaaaaa ttacttagat ggcttcctcc tgtacttttc
     2281 attattatcc ttataggaat gactatctta ggtaagtcct atatcaataa agtaacagct
     2341 cacaaaataa aactctataa ctctcgaatg actcctacta ttttaattcc aggatccagt
     2401 gctactcaag aacgatttaa cagcatgtta gcacagctca accaaatggg agaaaaacat
     2461 agcgttttaa agttaactgt caaaaaagac aatagcatta tctacaatgg acaaattagc
     2521 ggcaatgacc acaaacccta cattgtcatt ggatttgaaa ataatgaaga tggttatagt
     2581 aacatcaaaa aacaaacaaa atggctacag attgctatga atgatcttca gaagaaatat
     2641 aaatttaaac gttttaatgc tatcggtcat tcaaatggtg gcttatcatg gactattttc
     2701 ctagaagatt attacgactc tgatgaattt gatatgaaat cattgttaac aatgggaaca
     2761 ccttttaact ttgaagaaag taacacctca aatcatactc aaatgcttaa agatttaatc
     2821 agtaataaag gaaatattcc atcaagtctc atggtatata atttggcagg aactaattca
     2881 tatgatggtg ataaaattgt tccatttgct agtgtggaga ctggtaaata tattttccaa
     2941 gaaaccgcta aacactatac ccaactaaca gtaactggta ataatgctac acattctgac
     3001 ttgcctgata atcctgaagt tatccaatat gtcgcagaaa aaattcttaa aaatgagaaa
     3061 ggtaaattac caaaacctca ctaaagctgt aaaatagcaa cgcccctggt attttgatat
     3121 aatatattgc gaattaaact aaataaaaaa ggatattatt atgaaactca tttcatggaa
     3181 tattgattcc ctcaatgcgg cattaacaag tgaatcaaca cgtgctttaa tgtcacgcca
     3241 agttattgat actttagttg ctgaagatgc cgacatcatt gcgattcaag aaactaaact
     3301 gtctgccaaa ggacctacta aaaaacattt ggaagtactt gaaacttact tcccagaata
     3361 tgatttagtt tggcgatcat cagtcgaacc tgctcgtaag ggctatgctg gcacaatgtt
     3421 cttatatcgt aaagggttaa atcctattgt tagcttccct gaaatcgatg ctcctactac
     3481 aatggataat gaaggtcgca ttattactct cgaattggaa aactgctata tcacacaggt
     3541 ctatactcca aatgcaggtg atggcctaaa gcgcttaggt gaccgtcaaa tatgggatat
     3601 taaatatgct gagtatttag caacattgga tagtcaaaaa cctgttctag caacaggtga
     3661 ctataacgta gcccacaagg aaatagacct agctaatcct agcagtaatc gtcgttctgc
     3721 tggattcaca gatgaagagc gccaaggctt tacaaatcta ctagccaaag gttttacaga
     3781 tacttttcgt taccttcatg gcgatgttcc aaatgtttat agctggtggg cacaacgaag
     3841 tcgtacatcc aaaatcaaca atacagggtg gagaattgat tattggctaa caagtaaccg
     3901 tgtcgcagat aaaattacca agtcagaaat gattcattct ggtgatcgtc aagatcatac
     3961 accaattatt ttggagattg aactataaac ccaaaaaaag atgattgagt tataactcaa
     4021 tcatcttttt atactaagtt gaggtcttta tacttagtac gatagccaat ctgaagaatt
     4081 ttattatctt taactagcaa tggacgctta ataagcattc catccgatgc caataagtcc
     4141 aaagcttggt caagagttaa atcatcaaat ttatctttta agcctaattc tctgtaagaa
     4201 tttcctgaag tattgaaaaa ctttttaagg tcataaggcg aattctctaa caattctttt
     4261 aataacgaag ctttgggagg attgcttttt atatcaatag cttcaaaagt taagccgagt
     4321 tcagttaact ctttttttgc tgaacgacaa gtggtacatt tggggtattc ataaaacgta
     4381 tacatattat ttctcctttt cgtattctaa taaccaagat tttctttcta agccaccagc
     4441 ataaccagtt aattgaccat atcttcctaa aacacggtga caaggtatga ttagagaaat
     4501 aggattttta cctattgctc ctccaacagc ctgtgcagat ggacaattta gctctttcgc
     4561 taattctcca taagttttgg tatctccaaa agggattttc tgtaattcct gccgtactct
     4621 cgtctgaaat gctgtccctc tcaatgataa tggaatacta ggtaaatttt gctgtgaaaa
     4681 ataagcatct aaccacttct taccaagtaa agtataggaa tttgacatat taacaatttc
     4741 ctcttggttg actgctagca tatcatattt ttgcccaaca aaataaagcc ctgataaacc
     4801 tagattgtct gctagaaggc gaatttctcc aagaggagac tgatagaatt cttgatataa
     4861 catatgataa gcataactta aaaagccatg aaaagcaatt catgactttt ttagttcttt
     4921 attgttgttt tgcaattaaa cgtacacgga cgatattctc atcaccttca aattcttcaa
     4981 ttaaagtaga aatcttgtta ttatccgttt catctaaatc tattagtgtg taggcgtagt
     5041 caccctttga tcggttaata atgttatcaa tatttatacc tagctctgat acagcagttg
     5101 aaattttagc cacaatgtta ggaacattct tgttaattaa agtaatacga aatggtgcgg
     5161 tttggatttg atgtacattg gggaaattaa ctgaattagt gatttctcct gtctccataa
     5221 aacaacgaat ggtttgactt gccataatag cacaatttag ctctgcctca tctgttgatc
     5281 cgcctacatg aggaaagact gtaattcctt tttggtttaa taattctttg tctccaaaat
     5341 cagtaatata gcgcttgaca acaccagttt ctatcgcttt aaatagctct tggttattga
     5401 ctaattctgc acgagcaaag ttgataatcg tagttccttt tttcatgatt gaaaaagctt
     5461 ccgcatcaaa agtatgctta gtttcatttg ttaagggaac atgaattgtg atatagtcac
     5521 aagtttcaaa aatatcctta atctctttaa ccctttgaac atggcttgaa atattccaag
     5581 ctgtttcaat tgaaacatag ggatcataac caagaactgt cattcctaag cgtctagcat
     5641 cattcgcaat tctagctcca atagcaccaa ggccgataac tcccaatttt ttccctgcaa
     5701 tttcattacc agcaaaagct ttctttcctg cttcaatttg tttgggaata tctgttccag
     5761 ttagagtatt aacccatcgg ttagctccta aataatcacg agctgaaagc aataaggcag
     5821 caatgaccgc ttcttttaca gcattagcat ttgcacctgg ggtattaaac acgactattc
     5881 cctgtgcact tgcctcttca ataggaatat tatttgttcc tgcacccgcc ctagcaatag
     5941 ctttgaggtt acttgggaaa tcctgattat gcaaattttg actacgaata atataggcat
     6001 caggattttc tgacatatcc ccatcaatct ggaaacgatt ccctaactct tgtaaaccaa
     6061 tttgattaat attattaaat gttttgacac taaaaaccat tctttttcac taaggttatc
     6121 tctttagtca tcattgacta aaataacccc tttctttttt cttacatggg atttctttca
     6181 tcaatctcac gatagatgct cgtttctccc ttttctttta tcttctgata agccaatcgt
     6241 accccatcta gaggtacttt accacaatat tgatagccta atttatttag aatatgctgc
     6301 atagtgacat ttttttcatg agtatcgcaa cgaaaatcag gtcctttatg accttcaatt
     6361 aacccttgaa ggaatgtctg tgctagtcct ctaccacgaa attgatttga aatagcaata
     6421 cgatgaaatg taagatagcg atggttatca tggagccatt ttccctcata aatagcatca
     6481 taaacctctt catgaccatc aatgacagcc gcataagtcg ctagcatccc atcttcaata
     6541 ccaacccaag cataaccgtt caagatatca tcaataatat cgttacgatt agggtatcca
     6601 tcttcttttt gccattgatc acttcctgtt ttagcaattt cggcacgcgc ttcctcaata
     6661 agaagcataa tttggtcaat ttcatttgga aaagctaatc tgacttgcat tatattaccc
     6721 acttttatat attatagact tataataacg gtttaatttt ctttcgtgat aacatttact
     6781 atgcattttc tgcttcaaat gttttcataa aatcaattaa ttcgatgaca ccttgtttag
     6841 gaaaggcatt ataaaggcta gccctcatac cacctaccga acgatgccct ttaatatttt
     6901 taaatccacg ttctgtcgct tcctttacaa atttttcatc taaatcttta ttaatagtta
     6961 tgaaaggaat gttacataga gaacgactct ttttatctct gacaggatta ctataaaatt
     7021 ctgaggaatc aatataatca tagagaagtc ctgatttttc acggttagct ttttccatag
     7081 catcaacacc acctagactt ttcacccatt caaagactaa tttagcaatg taaatactgt
     7141 aagcaggcgg cgtattataa agagaactag catctgactg aatcttataa tccaacattg
     7201 atgataaagt tggttcctca ttaatcatat cttcacggat aataactacc gttacaccag
     7261 caggtccaat atttttttga gctcctgcat aaatcattgc aaaatcttct actttatatt
     7321 ttacagctaa gatattagaa gacatatccg caataacagg cactccatta gttttaggaa
     7381 tatcataaag cgatgttcct tcaatggtat tatttgttgt cacatgaaca taggcagctt
     7441 caggatcaat ctctttttca tcaaactctg gaatataatc ataaactgat tcttctgatg
     7501 aggctaatag aattggttca aaaggaattg tttttgatag ttttacagcc tctgtataag
     7561 cttttttccc ccacgaacca gcaacatgat agtaagcttt acgccctctg gcaatattca
     7621 agggaatcat actaaactgt aaggatgcac caccttgtaa aaagattact ttataattat
     7681 ccggaattcc catcaaatct cttaagtaac gctcagcacc tttgataata tcatcaaact
     7741 ctttggagcg gtgcgatacc tccaaaacac tcatactgga cccttgataa ttaaggagtt
     7801 cagattgggc ttttacaagc actggttttg gtaagactgc aggacctgca gaaaaattat
     7861 aaattgtcat ataacacctc catataattg tgtaagatta tagcacagta ttttgaatat
     7921 tgtaaagatt tttttgatta attttgcttt ttattcgtga tataatggta aaggcgaaca
     7981 atcaagttta aaggagtaat ttatgatttt gagggaattt tgtgcagaga atttaacaga
     8041 cttaaccaga ttagataaag ctattatttc acgtgtcgag ctttgtgata accttgctgc
     8101 tggaggcact actcctagct acggtgttat caaagaagct aatcaatatt tacatgaaaa
     8161 aggtattagt gtcgctgtta tgattcgtcc ccgtggagga aattttgtat acaatgattt
     8221 agaattacgt attatggaag aggacattct acgtgctgtt gagttagaat cagatgctct
     8281 tgttttggga atattaactt caaataatca tattgatact gaagccattg aacaacttct
     8341 acctgctacc caaggtttgc cacttgtttt tcatatggca tttgatgtta tcccgaaatc
     8401 tgaccaaaaa aaagcaatag atcagttagt tgctttaggc tttacaagaa ttctcctaca
     8461 tggttcatct aatggcgaac caattataga aaatatcaaa catatcaaag cattagttga
     8521 atatgctaat aatcgtattg aaattatggt tggcggcggt gtgactgctg aaaactatca
     8581 atatatttgc caagaaactg gggttagaca agcccatggt actagaataa ctcaataaaa
     8641 aattcgaaac agggctataa gtttctttag tatatttact actaagttct ttcatgtttt
     8701 gtaaaacctg aaagaaccgg aataagagca taccatcttt tataacctgc tctcagaaat
     8761 aaaatccaat ttgcaatggt attcaaaaga gctgaaatta aaaatcttaa ggaaattgga
     8821 agcaccttac ttctttattc caatagctaa gtcggagatt ataaaaggtt gccaaagaaa
     8881 actttggcaa cctttttata ttattactta taaatcatgg aaactagcat agagttcttg
     8941 tctattgaga ttaagttctt ttgctacttt ttttatcgct tgattagttt tatcgccatt
     9001 agcgatatat tcttttacta atactagtgg atcttgttgg ctactgtctt tcactcgctc
     9061 ggtatctctc ttaccatcaa caataattaa gcattcacct ttgagaggga ctttttcaat
     9121 atgccctaaa agttgactaa tggttcctct ttgatactct tcatagagtt tcgtcaattc
     9181 gcgtactaaa acaacttggc gatctccgta aatctctttc atgtgtttta gcgtatcaga
     9241 gactcgaaac ggtgactcat aaaagatttg tgtttcaggg taatctttct ttgtttcaaa
     9301 aaaagttatt tgttgacctt tcttacgcgg taagaagcca taaaaaatat gaggttgtgg
     9361 agctaaacct gaagcgatga gagcagtaat accagcgcta gctcctggta tagatacgac
     9421 cgggatcccc ccttcaatag cagccttgac aaggtcatgt cctgggtcag aaatagaggg
     9481 cattcctgca tcagatactt gggctaaaga tctcccttct tttaacaaat caattaaccc
     9541 agagatttta tcgtaagcat tgtgttcgtg aaaactaatt tgtttagtag taatatcaaa
     9601 gtgcttgagt aaaagtcccg tatttcgtgt atcctctgca caaataaaat caacttctct
     9661 taaaatccta atggcacgaa aagtcatatc atctagatta ccaattggag ttgggactag
     9721 atagagtgtc ccgtaatgtg tatttgattt aaaacttttt tgaacttgca tttccatcaa
     9781 tctctatata ataattcaat acaaaaagca cattcttcgt cattttcacg acgttgacca
     9841 tagaaagtat tacaaatgtg aaagccatca ttgtaaatgc cttcgagtaa ttctcttcct
     9901 tgtttagaat ttttaactgc agtttctgtt tctgcttcaa taacgctcag acgctcacga
     9961 agtttaccat tttcaatacg tagaacagta ttctcctcca ataatttttg gatttgtttt
    10021 ttcatagtct caatttcaga caacccaact aataaatttt gtgaaaaatc atcaaaggca
    10081 tcaaacaagt cttttttatc cattcactct tcctctctgc gcgtattatt tgtctaagaa
    10141 agcaccatat actctaaact attttgaaaa ctaacattgc tcttccacat ttgctgagct
    10201 ctataagtat tttctaaatc ataaagttgg ccttctttag ccagcattat tgtcagccat
    10261 tgaaaaacaa atgcctgatc atttttatct acaacttgcg atgtcaagcg agatacttct
    10321 aaataagcag tttgcctatc ttttgccaag gtcgtcacaa actgttgact taaattcaag
    10381 taatctaata actttttgtt agccaacaat atttctaaat gtttaggatc gtctgctact
    10441 tgagctaata agctagcttg tgattttagc aatcctgctt gttcagcttg atgaaccaac
    10501 atgtccaatt gttttagaaa acgaaaaatt tgcgttctac tcttaattgt tggcaaaaca
    10561 ttgttttcat cattagtcaa taagatgaca taagatgaac tttgaggttc ttcaataaat
    10621 ttcaaaaggc tgttggctgc attgacatgc attttttcgc aatctttgat gataaagacc
    10681 tgtgatttcc cttcgaaacc tgatcttgaa aaatctttag tcagctcttt tatagtctct
    10741 gtcttaataa gttgcccctg tggttctatg attttaacat ctgaaaattc tccattagcg
    10801 attaaacgac aagctctaca cttttggcat ggtaaaccac tctggcgctt ttcacagaac
    10861 tgactttgtg ctaaataaag tgccatatct agacttgcaa aatttcccga aaaaagatag
    10921 gcatgactca tcctatctga ctgtaaaata gtattaaact tttccagcaa tttgggttgt
    10981 gttcgcttta aatccatgac ctttgtactt cctgattcat tggttgatac gatctaaaat
    11041 aatagagaat gtctcagcta ttacttcatc tagctgttgg gaggcatcaa tcgtcacaat
    11101 gcggtttggc tccgaatccg ccaattcaag gtaaccttga cggacgcgtt gatgcatatc
    11161 taattgctcg agatctagac gatttacctc cctttgaaca cttttttgaa tacgctctaa
    11221 acctacttca gaaggaacat caaaatataa agttaaatca ggtttatgac tatcagtcgc
    11281 atagtcatta agccatttta tatgtgactt atctaatcct cttcctgatc cctgataggc
    11341 aacagaacta tcaataaaac gatccattag gactattttc ccttcttcta gagctgggag
    11401 taccttttcc actagatgtt ggcgtcgagc agccatgtaa agcaatagct ctgttttttt
    11461 atccatttgc gtgtgtttaa catctaaaat aatatgacga atttcttctg aaatagttac
    11521 gccaccaggc tctctggtgg tgagaatatc ttgtgataat ttttctctga gcaagggtaa
    11581 aacagcttct aaaacggttg ttttacccgc accatctggg ccctcaaaag aaatcattag
    11641 tccttttttc atggtacatc ctttatttat aattactact attctatcaa aatttatcaa
    11701 aaaaagacac ccacaacttg attgtgcatg tctgatattt ttaagaaagt ggttttttca
    11761 cggtcttttc taaagtttcc acaataacat catcaaatac tagttcttgt tttaaaacat
    11821 ccttgtcagc gtatccttct agttgtatct cgaccgcttt cttacctgct tttcgttcaa
    11881 taatgactaa acgtttaaca cttaaatttg cactagaaag acgatttagg acttttgata
    11941 gtacccctat cccctcatca gctaatataa ccaagcgata accttcttga ccataccccg
    12001 caatttcaag gaaggcttta aaaacatccc tatccgtgat aatcccataa agttgtccat
    12061 tatcaactac tggtaaaaca ccgatctttc gagacatcat caaataaata gcgtcctcaa
    12121 ggctagcata ctgtgaaaca gtcacaatat ctttaatcat aatatctcga atttttgtct
    12181 tattaagaag atagttcatc tcatatatag aaagactggt tgctttagaa ggttgtgctt
    12241 ctgccattgt cccttctgtc actaacccca atagctgatc attttcaaca actggcaaac
    12301 gccttaagtg gtgttctctc aataaatcag cagcttctgc aactgtagtg tctggagaga
    12361 cataaactaa tttttttgtc ataaaatctt tcactggcat acctgactcc tctaaatatt
    12421 atccacctaa gtaagcttta cgaacttgat ctgaaactag taattcctta cctgtccctg
    12481 ataaaacaac cttcccagtc tctagaacat atgctttatc agcaatcgtc agagctttat
    12541 ttgcattttg ttcaactaaa agaactgtag tcccttgttt tttgatgtct tcaatgatat
    12601 taaaaatttc ttgaataaaa attggagcta ggcccattga cggttcatct aaaagtaata
    12661 atttaggacg actcatcaat gcacgcccca tagcgagcat ttgctgctca ccacctgata
    12721 aggttgctgc atcttggttt ttacgttctt ctaagcgagg aaaacgatca aacacttttt
    12781 ttaacatttt ttgattttgt tcacgatcct tttgaagaaa ggctcccatt tctaaatttt
    12841 ccataacaga caaacttgag aaaacatggc gcccttctgg aacctgagct aaaccattgc
    12901 caacaatttt tctagctgct aacttatgta taggtttccc cataaaagaa atagaaccct
    12961 gacttggtct tactaaacca gaaattgtcc tcaaaattga tgtctttcct gtaccattgg
    13021 ctccaattaa ggttacaacc tcaccttggt taacttcaaa cgagacatca ttcacagctt
    13081 gaataacacc ataatgaata gatagatttt ctactttcaa catggtcatt ataactcacc
    13141 ccccaagtat gcttctataa cacgtttatt atttttaatt tcttcaggtg tcccgtgagc
    13201 aatcagacga ccatattcca atacataaat tctctctgtt acttgcataa ccaagttcat
    13261 atcatgttca attagcataa tagtaatatc aaaatcactc tttatttgac taattaactg
    13321 cgtcaattct gcagtttctt gaggattcat tcctgccgcg ggctcatcca aaaacaatat
    13381 ttttggttct gttgctaaag cacgaacaat ttctaaacgc cgttgttttc cataagggag
    13441 gttcttagca agagcatctt gatatgcttt caaaccaaaa atctctaata gctctagtgc
    13501 ctttttcctt aaggcttttt cactatgata gtatttgggt aatcgtaaaa aactagcaat
    13561 aggatgtgat aagtgatgat tactgagtcc tactagtaca ttgtctaaaa cagtcatatt
    13621 tttaaaaagt cggatatttt gaaaagttct acctaaacct aaagaagcta ttttagctgg
    13681 tttcctccca tttaatatct taccatcaag tgatattgtc cctttactag gaaggtatac
    13741 tcctgttaag agattaaaaa gcgttgtctt acccgcaccg ttggggccga ttaagccaat
    13801 taattcacct ttatgtaatt tcatcgaaac atcacctaca gcagttaggc caccgaaatg
    13861 tttacttaaa ttttttactt caagaagtgc catctaattg cctcccttac tcgtcttacg
    13921 aaatagatga gatagagtta actccttcgt tcctaataaa ccgctaggtc taaaaatcat
    13981 aattaaaatc agagctaaag catagataat cattcgaaca gaagcgtagt cttgtagtaa
    14041 catattcaat aatcctaata aaattgctgc taaaactgtc cctgtcattg aacctaaacc
    14101 acctaaaaca acaataatta aaacattgac agacatcata aatgaaaagt cttttggcat
    14161 aacagttcca acgtagccag cctgtaaact accagcgata gaagctaaaa tagctccaaa
    14221 aacaaaaaca attactttca cttttgttgt atccacaccc atagactctg ctgcaatttc
    14281 atcttctcgt atagcaattg tattacgccc taagggacta cgcaaaaaat tcattgcaag
    14341 gataagactc acaactgcca ccccataaac taaagaccag gttgtaaaag gcggaatccc
    14401 catgataccc gcagcaccat tcgtaatatc acctccatta accaacaaaa tacggataat
    14461 ctcagcaaca cctaaagtag ctatagctaa ataatcacct tttagtctca aagtaggaat
    14521 tcctacacct aaggcaatac ctcctgcaac aagagttcca acaagtatag ctatcaaaaa
    14581 acctacataa gttggcattt taacgccgat aatagcaact gcataagcgc caatagccat
    14641 gaaacctgca tgtcctagtg aaaactgtcc cgaacaacct acaattaggt tcaaaccaac
    14701 tgctaaaata acattaatcc caattgtttc aataatttga atatgataga ggttaaatat
    14761 acccatagat attagcacgc taatcaagag ataccctaac actactatag ctaaccaaga
    14821 taaaattgat ttcatatttt ctttcatgcc tagaccttct ccttaatatt ttttccaaga
    14881 attcctgctg gacggatcaa gaaaatgaga attaaaatag cataaacgat accatctcga
    14941 aaatctgaca caccaagtgc agtcgctaaa gtttctaaaa tgccaataac aaaaccaccg
    15001 atagccgctc ccggaatgat accaatacca cctaagacag ctgcaacaaa tgctttcaac
    15061 cctggagtta cccccataag aggttgaaca gaattataat aaagcccaat cagtactcca
    15121 cctgctccag ctaaagcaga ccccagcgca aatgtaaaac taatggtacg gttaacatta
    15181 attcccatca attgtgcggc atcgctatcc actgataaag ctctcatagc tttccccatt
    15241 ttagtttttt gaacgatgaa ctgtaaagta agcattagta atagcgcaat acctaaaatg
    15301 atgagctgaa cattggttat agttatcggt cctaaattat atttaacagt atgaatggct
    15361 tgcggaaatg ccctagtatc cgctccaaca aggtatacca tcccgtactc caaaagaaaa
    15421 gaaacaccaa tagctgtaat taacgcggca attctagttg attttctcag tggacgataa
    15481 gctaaatatt caattacagc acctaggaaa gcagacccca gcattgcaat taataaagct
    15541 aggaaaaaat taagatgaag atgattaatc aaataatagc ccatgaaagc gcccatcata
    15601 tagatgtccc catgagcaaa attaatcagc ttaataatgc catataccat agtatatcct
    15661 aaggcaagta aagcatatat ggatcctaag ataagtccat taatgagctg ttgaagcata
    15721 ttataaccat actttctatt atatattatt gtcgaattat tctgaaaaat tactttttaa
    15781 aaaaggggag ctgaaaatta aactcccatt tttctagaga atacttattt tgctgaaata
    15841 atgttaacgc tacttgtttt tccatcgtct aatttgacaa cgtaagctga tttaacaaca
    15901 ttatgatttt tatcaataga cattttccct gtcacacctt taaaatcttt cagcttagct
    15961 aaagcttttt taagatcaat tgaagtttta gctcctttag cagatttagc tgccatatac
    16021 acggcatcgt atgataaagc cgagaacatg gatggttctt caccatatgc cttaacgtaa
    16081 tgatcatgga aagctttagc tttggttgat ccttgtgtag tgaaacctgt caaataataa
    16141 acgtttgaag ctcccacagg tgttgccgat tgcacaaatt tcggactatc aaaaccatca
    16201 ggcccaagaa ccggtttaga gatacctaaa tcacgcgctt gcttaactat taatcctgtc
    16261 tcggtatagt aacctggcat cacaatagca tcatattctt tccctttcaa cttagtcaat
    16321 gacgcttgga aatcagtatc accagcggag aatgtcatgc tatcaacaat ttttccacta
    16381 taactttcct taaatgattt tgctaccccc tttgagtaat ctgatgaatt atcataaaat
    16441 agaacaactt ttttagcttt aaggttgtct gtagcatact gagacaatag ctttccttga
    16501 taattatcga caaaagtagc tcggaagaaa tactgattta aggtttttcc atcagaacca
    16561 tagactaaat tgtcttgtgt cgcagcaggt gcaatcattg gaactgctgc tgcatttact
    16621 tttggagttg aagatgcagc tgcacccgat gttgctggtc cgataatgac attagctcct
    16681 ttggtaacca agtttgtggc aaccgttgct gcctctgcat tttctgattt attatcctgt
    16741 gataggactt ttaacttctt gccgccaacc cctccagcag cattaatttc ttttactgct
    16801 aagtcagctc cattcttcat tgaatttcca tacgaagaaa cgtttcctga taattcccag
    16861 ttatacccta ccttaatatc tttccctatt tttgttcctg tcgaagatgc tcctcctccg
    16921 acatttccac atgctgctaa aagaactgta ctagctaaaa caagagctcc taaactaagt
    16981 ctcttttcca tatttttctc cttttttgaa aatggtaact aaaataatac agaattatca
    17041 gaatattgtc aatagttttt aaattatttt tttgaaaatt atagaaacta aataaaaaag
    17101 aaggacatcc gaactccttc tttctaataa ctactataag tatcttagcg atataagttt
    17161 ccaacaaaat cctgatcaat atcatcaatt gctgataacc gaacatcctt aacaaatttt
    17221 aaatgtttta tctctttact aacgctaaca atatcctctt tattaacata aatgactaga
    17281 tatcttaatc tacgagaatg ataatgaaca tctccgtatt tatataattt acgagcgtca
    17341 cgattatagt aaagataaac gctcattcct aaacgctctt gtttattcaa taattttttc
    17401 ctttctgcgt catatctgta attactaaga ttataacata attccaacaa ataaaaaaag
    17461 agttgataaa ctcaactctt ttaaatctat tattgtagat tattattttc cataatagca
    17521 tcaataaagc cataatcaag tgtttcttga gcactcatcc aacgatcacg ctctgcatca
    17581 tcatggactt tttcaataga ttgaccagaa ttatcagcta agattttttc taaagtatga
    17641 cgcgttttta aaagatgctc agcagcgata gccatatcag attgctgtgt gcctccgcct
    17701 gttccaccca ttggttggtg gatcatatat tctgcattcg gtaacataaa acgttttcct
    17761 ttagcaccac tcgaagcaat aatggttccc atcgaagcag ccatccccat aacaatcgtc
    17821 tgtacgtccg atttaataaa gttcatggta tccacaatag caagtccagc cgatactgaa
    17881 ccacctggtg tattgacata aaggtaaata tcctttgtat tatcttgtgc atcgagaaac
    17941 aataactgtg caatgatact attggccata ttatcctcaa cttggcctgt caacataata
    18001 atacgatctt ttaaaagacg tgagtaaata tcataagagc gttcaccacg acttgtttgt
    18061 tcaataacta ctggaatcat atctttctcc atttcttttt atcactgtca ctattataat
    18121 catttggtca acaaaggtca aataataact tttttaagaa aaggctttct gagttcttca
    18181 gaaaagcctt attcatctca aatcttattt tgtaccaaat aagcgatccc cagcatctcc
    18241 gagtccagga acaatgtagc catgttcgtt gagtttttca tccaaagcag cggtataaat
    18301 atctacatct ggatgtgctt cttgaagtgc tgccactcct tctggagctg ctactaaaca
    18361 aacgaatttg atactagctg cgccacgttt ttttagagaa tctactgcta aaatagcaga
    18421 cccacctgta gcaagcattg gatcaactac aaaaatctga cgttgatcaa tatcttcagg
    18481 taacttaact aaatattcga ctggttggaa agtctcttca tcacgataca taccgatatg
    18541 accaacttta gcagctggca caaggcttaa gaaaccatca accataccaa tacctgcacg
    18601 taaaattgga acaattgcta actttttccc agctaattgt ttttgtacag ttgtcgctac
    18661 tggtgtttgg atttcaacat cttctaaagg caaatcacga gacacttcat agcccattaa
    18721 catcgcaatc tcatcaacta actcacgaaa atctttagta gatgttgtag tgcgacgtaa
    18781 gattgacagt ttatgttgaa taagtggatg tgaaatgact tgaaattttc ccattataat
    18841 gaccgctttc tatttaattt tgagcattaa aaacattcta acgcttgttt acattcaaag
    18901 tattataaca taaaaaccct ttctatgcga ctttttttat agtctagtca ttttttctaa
    18961 agtttgtcac taatcgcaaa actgcctcct caataacaga cctaggagct gcgacattga
    19021 ttctagcatg ttttttccct tcttttccaa aagttaaacc atcatttaat attaatttgg
    19081 catcatatcg aagtttttct tgaatttcta aatgcgttaa attataagca gaaaaatcta
    19141 accaaacaag ataagttccc tctggtttca tcactttgat atgagttttt tgcgttaatt
    19201 gctcataaag gtattctatt gatccttcta actccatttt tagggccttt aaccattttt
    19261 tcccttttgt aaaggcaact tctgtagcta ataaccccaa tgaggaaatt tcttgttgat
    19321 tgttagctat ttgcctcttc ttaaaatcag agcgtaactt ttcattttca attattgcaa
    19381 aactattttt agtccctgca atattaaacg ttttagttgc actagataaa ataacactaa
    19441 attctttaaa actagaatca acagtattga agctatggtg tacattatca aataatacca
    19501 aatcttgatg tatttcgtca gaaacgagga taacattata ccgtttacag atatctccta
    19561 ttttttgaat ttctcctttt gtccagacac gtccaccagg attatgtgga ctacaaaaaa
    19621 tataaagctt tacattattt tcaatgatat ctttttctaa ttgtttaaag tcaatctcaa
    19681 aatactgatt attattaagt aaactatttg aaactaaatg acgattattg tattttatag
    19741 ttctagcaaa aggcggatag actggggtat taattagaac agcatctccc tcctttgtga
    19801 aggcttgtat ggcaactgaa atcgctggta taaccccatc aattaataat aatgattttt
    19861 tatcaaattg ataaccatgt tcttgcactt cccaattaga gatagcttgg tataaactgt
    19921 ctttaggact atcatagcca aaaaccattt ggtgtgaaaa atcttcaata gcctccgaca
    19981 tttctggaaa gataggaaaa tccatatccg caatccagag gggaaggatt tcttgatctt
    20041 tttgaaccgc cttccatttt atggcattac ttgaaaaacg ttctggtaaa cttgtgaaat
    20101 catatgtaat cataattact cctgactatt ctacagctaa aaatatccct tgtcgccttg
    20161 gtgactattt taccatttgt ttttgatact atatactaat taacattcta aagataaaag
    20221 tgaccatttt aaacgatcac tttctttatt attttaattt atcattttca taagtatggc
    20281 ttaattctaa attaggaaag ctttgctgcc taagggcttc ataaacaatc atacaaacag
    20341 tgtttgaaac attcaagcta cgcacatgct catcgttcat gggaattcgt aaggcctttt
    20401 cggcatgctc gcgcataaaa gtttctggca atcctttatc ttcacgacca aacataaaat
    20461 aatggtcttg atcatcatta taattttcat ctgagtaaac cttgtcagca aatttgctaa
    20521 ttaggtgaac tttccctcga catgacaaca taaattcttc caaactatca tagaaactaa
    20581 catctagctt atcccagtaa tccagtcccg ctcgtttcat ttttttatca tcaataggaa
    20641 atcccattgg tcgtatgatg tgcaaaggag catttgttgc cgcacaagtt ctagcaatat
    20701 taccagtatt tgctggaatc tgtggttcaa ataaaacaat atgatttcgt ccgctatcgg
    20761 aacgatgatt tttctgcgtt agtgtttcaa tattcatctc atcatcactt tctagaactc
    20821 attctgactc gcaaaaaaac cacactgctc ggagtcaatc tcagcaaaca atgtggttta
    20881 tggtggtttg ttaacttaaa tcataacagg tttgatttaa actaacaaat cgttgacact
    20941 attatagcac ttcagtctac aaagtcaacc tattcatgag tttttgggtt atttttttga
    21001 tagatataat gacaaatcaa acagaataat aagcaaaaac attggaaaat taaaaccgta
    21061 aagaaattac ttaacaaact cccgataaag aggatcaaac cgcctaaaat tgctagaatc
    21121 ggtgaaacta agccggtaaa tttattttta atatcttttt tgagataaag gctgagtact
    21181 aaaacataaa gaatgattaa actagtatta ttaaacacca cagcaatttc actaatatca
    21241 ctacccggca ataaattaaa ttttgtaacc ataaagtgaa caaaaagcca aaaaatagct
    21301 acagccatca ccgtcaaact agctggcaat gacatttgat acttcaaatt tatgttagcc
    21361 atcctctcac ttttgatcca tccgcgttcc gcaaaagctt gaggtagacg catcgtccca
    21421 agtaaaagtc cattggcaac acctaataca gataaaataa caatgaaaga taataaacgc
    21481 ccaacactag gaccgaaaat aatgttagct gcataattaa tagcatcatt accggttgtc
    21541 ataataaagc tagcacctaa aatttgtgtc aaaccataga aaaatgccaa atatgataat
    21601 aagataagcg ctggaccaat gacaaaggca agtggtaaat tcttcttggg attcttgact
    21661 tctggtgcaa tactaacaaa aattgtccaa ccatcatatg caaaatacaa tggtaccaga
    21721 cctgacaccc aactccatcc aacatttgac ggctgaatag ctgttaaatg ttgtggaagt
    21781 tgtggagctt tatcagacca aaaagcacca attaaagcaa ttaggactaa aggaatcatt
    21841 tttacaaagg tcgttaaaga ttggaatccc cctgcaatac gcttagcaaa gatattcatg
    21901 agactaagtg ctaaaatata aacagcagct aaaataatct gtgattccaa acttgatgat
    21961 tcacctaaag tataaaatgc agctacccat gaaacgattg ctgttaaagt tggaagatac
    22021 aaaaaagacg caaacaagcc taaagttgct gctaaagctg ggctaacata tttttcgtag
    22081 taagaaaaaa taccaccact ttcactcgtt ctaatagcta gctcagaaat tgacaaactt
    22141 ccaaaaacaa tcgaaaaaga acctagtact aaaattacca tgcctaaaaa gacatctcca
    22201 ccagtaaatt taaggatgtc atcaacttta aaataaatgc ctgaaccaat cactacacct
    22261 acaatcatag caattgtagt cattaatcca taggtctgtt taatagaatt atgattctcc
    22321 ataatatctc catttctact gtacgcttaa aggttaatta taacctaatt tagggcttgt
    22381 cacaagttta tctgtgttat ttttcaccag attctgataa gagaatcata ttactcttat
    22441 cagaaagatg aaaacgtttt ttaaccataa ttctcgtgca tttttactaa aaaaaaggta
    22501 tcatagagtt tattattgga ggtaaaaatg agaataattg ttgtcggcgg aggtaaagtc
    22561 ggaaccgcac tttgtcgctc acttgttgct gaaaagcacg atgttgtttt aattgagaaa
    22621 aaagaaaacg tccttaaacg tgtcactaaa cagcatgata ttatgggaat tgttgggaat
    22681 ggggcaaact ataaaatttt agaacaagcc gaagtcaaaa attgtgatat ttttatcgct
    22741 atcactgatc gagatgaagt taatatgatt tcagcagttc ttgccaaaaa aatgggggca
    22801 aaagagactg tcgttcgcat gcgtaatcct gagtattcta atccttactt caaagataaa
    22861 aactttcttg gcttctcttc agttgtcaac cctgaattgt tagcagcaca atatatcgct
    22921 aataccatag aatttccaaa tgcaacttca gttgaacatt tcgccaacgg gcgtgtgatg
    22981 ttaatggaat ttaaaatttt agaaggaaat aaactctgtc atacttcaat gagtcaaatt
    23041 cgtaaaaaat ttggtaatat tgttatttgc gcaattgaac gcgatggcaa attgattatt
    23101 cctgatggcg atgcaactat ccaagtcaaa gataaaatct ttgtaactgg aaaccgtatt
    23161 gaaatgatac ttttccataa ttacgttaaa aataaagttg tcaaaaactt gatggttatc
    23221 ggtgcaggac gcatcgccta ctacctactc aatatcctta aaaataccaa tacacacgtc
    23281 aaactagttg aactcaacca agaacaagct gaatatttta gtcaagaatt tcccaatgtt
    23341 cctgtcgttc atggggatgg tactgctaaa aatattttgt tagaagaaag cgttacaagt
    23401 tttgacgcag ttgctacttt aactggtgtt gatgaagaaa atatcattac ctcaatgttc
    23461 ttagaaagta tcggtattcc taaaaacatt accaaagtta atcgaactag cctactagaa
    23521 atcattgacg ataaacaact atcaagcatt atcacaccca agcgtattgc cgtagaccat
    23581 gtgatgcact ttgtacgcgg gcgtgtaaat gctcaggatt ccaacctaga agctatgcat
    23641 catattgcta acgatcgcat tgaaacctta caatttgaaa taaaagaaac tagcaaatta
    23701 gcaaatcgta gccttgcatc tttaaaatta aagcaaaata ttttaattgc agcgattatt
    23761 cgcaataata aaacgatttt cccaactggt gaggatgttt taacagtggg tgatcgtatc
    23821 gttgttatca ccttattaaa aaatattact agaactagtg atatgttagc gaggtaggaa
    23881 tatgaataaa agtatgattc gtttccttct ctcaaaactt ttgctgattg aagctgctct
    23941 tctagcgatt cctctaactg ttggacttat ttaccgtgaa ccgcaaagtg tcatgatgag
    24001 tatagtcatt accatgatta ttctaatcat ccttggatta ctgggttctc tctttaaacc
    24061 taaaaattat catatctaca ctaaagaagg catgttaatt gttgcactct gctggatact
    24121 ttggtccttc tttggcgccc ttccatttgt tatttcaggg caaatcccta atattatcga
    24181 tgctttcttt gaagtatctt ctggttttac gacaacagga gcaactattc ttgatgacgt
    24241 cagtgtctta tcccccgcgc tcttattttg gcgtagcttt acccatctta tcggtggaat
    24301 gggggtttta gtatttgccc ttgccatcat ggaaaattct aaaaatagcc atttagaggt
    24361 tatgagagct gaagttcctg gacctgtatt tggtaaagtt gtctcgaaac ttaaaaaaac
    24421 tgcacaaatt ttgtatttgt tataccttct tatgtttgca gtatttgcag taatattata
    24481 ttttgcaggt atgcctttct ttgatagtat tatcatagca atgggaactg caggtacagg
    24541 tggttttgca gtttacaatg atagcattgc ccattataat agtcctctga taactaactt
    24601 agtttctatt ggtatgctta tcttcggggt taactttaat ctttactatc ttcttttact
    24661 ccgaaaaatt aaagcattct ttggtgatga ggaactaaaa aactatctcc gcatcgtggc
    24721 aatagcaact ttcatgattg ctctaaacgt catcggtatg tatgataatt ttcgacaagg
    24781 cctcgaacat attttctttg aagtatcagc tattattacg acaactggtt ttggcgtgac
    24841 tgatatcact cgttggcctt tgttttcaca ggttattttg ctattcttga tgtttatcgg
    24901 tggttctgct ggctctacag ctgggggctt taaagtcatg cgtagcttaa gcctagcaaa
    24961 gattgcacgt aaccaagtat tgtctacact ctatcctaat agagtcatgt cactacacat
    25021 taataaaagc gttctagata aaaatacaca acatggcgtt ttaaaatatc taactatcta
    25081 tttagctatc ttcatggcac tcgttcttgt tttaacgctt gatactaatg attttttggt
    25141 tgttgtcagt gctgcagcct catgttttaa caacataggc ccactcttag gtagcaatga
    25201 aactttctca ttctttagtc cattctcaaa actccttctt tcatttgcta tgatagcagg
    25261 acgtttagaa atataccctg ttttattgat gtttattcct aaaacatggt caaaactata
    25321 attacaaata ataaaacgga tatatcaatc tgatatatcc gttttattac gtcttaagct
    25381 aaaatgatca gggacaggat catttcctcc gtgggctaag ggatgacatc gcaaaatacg
    25441 tgcaatcccc atcaacacac cttttagacc atgtttttga atagcttcta tcatatacgt
    25501 agagcaagtt ggacgataac gacagctagc tgggaaagct ggagaaatat ttttttggta
    25561 aaagcgaacc aagaaaatca aaaaagattt aagcattaag atttattaat agccgcatta
    25621 tgcaactggc taatttcttt tttatttaat cgtcgtgctt ccccaggacg gagacccgtt
    25681 aaatctaagg taccgaattg tgtacgagat agcttgtcca ctaataaccc aacttgttca
    25741 aacattttct taacttgatg attacgacct tcatgaatag ttaactcaac tactgaacga
    25801 tttttttcat gatccacttt tatgatatta tagcgagcag gttttgtttt cttaccatca
    25861 ataacaacgc cacgcgttaa tggtcgaagg ttttctttag tagctattcc tttgacacgt
    25921 gctaaataaa ctttatcaat ttcattacga ggatgaatca ttttatcagt aaaatcacca
    25981 tcattagtca aaattaaaag tccagttgta tcccaatcca agcgaccaac aggatatatc
    26041 cgttctttga cttgtggcaa caagtcaata actgtcttac gtcctttatc atctgagaca
    26101 cttgaaataa caccacgtgg tttatttaag agataatata ccttctcttc gttgtaaatt
    26161 ggacttcctt caatttcaac cagatcacca gcctttactt tggtcgccaa ttcattaacc
    26221 acttgaccat taatcgtgac cattccttgc ttgattaatt cctcagcttt tctacgactt
    26281 gcaatccctg catgcgcaat atatttatta attctcattg ttttccttat cttctgtatt
    26341 aattgaatcc attgaaaata acgatacttc ttcgtcaact aattcaattg aagaggcgtc
    26401 aattaagtca tctaactgat taattcccat ataatctaag aaataattag ttgtctcata
    26461 aaggttagga cgtcccaaca cttctttctt tccagcctct ttaattaaac caaaagctat
    26521 cagtctcgtt atagctccac tggaattcac gccacgaata tcatcaattt caatcctagt
    26581 aataggttgt ttataagcaa taatagatag aacttccaaa cttgcccgag atagactctg
    26641 attaatcgga gccttcgcat aatctctcaa caaagtcata taactatcct ttgttaccaa
    26701 tttataagtt tgtgctgtct caagcaataa tagactagaa tcgtcatctt cttcatatcg
    26761 tttagccaac ttttctagtt gctgtattaa tgctgatggt gtcaaagaca ataattctgc
    26821 catttgacgc aaactcagtc catcttcacc tgcaacaaaa agaagagctt ctattgagcc
    26881 taaataagtc atttctcttc ctttcttaag ataacttgac taaaattact gtcttgttct
    26941 actgtgattt gatgtaattt tatcaactct aacattgcca aaaataaagt tatcatttca
    27001 gattttgttt ggcaatccgc aaacacttct tgtaatgtcg ttttcttttt aagattaaaa
    27061 tgtctttcaa taacgatcat catatcttca atacggtagt cttctttctc aattaccgtt
    27121 tgactatttg acaattcttt ttgtttttgg gacatcattt gagaaaaagt caaaaaaagg
    27181 tccatcaccg atttatcgtg tagaagaatt gcatcttcaa agataacttc ttgttttggt
    27241 tttgaaaaat atttggcacg ttcctgatgc tgattagcta actcttcact tagcacttta
    27301 aaacgtctat attcctctaa ttggctcaac aattccattt ctggatcatc ttcaatagga
    27361 gttgactcca ctactttagg taataagtta cggcttttta tcaacatcaa ctgacttgcc
    27421 ataagcatat actcaccagc tacttctaga cgcatcgcct gtaatgttgc tatataagca
    27481 aggtactgct caataacttc cacaatcggt acatcgtata tatcaacctc atatttagat
    27541 acgaggtgta agagcaagtc taggggcccc tcgaaatctt ttaacttaat atccattatc
    27601 tataatatct ctctaaagtg ataggagttt ttaagcctag aaggcggctg agctccgtca
    27661 ttgttttgcc attttctttt tgctttagaa taaattgttc tcgcagaagt tgtgcagtta
    27721 gttgttgctc attcttctca tttaagaaat ctgttaattg attaaaaaac cattgtctag
    27781 aataaggctt accgttatgc tcaaagacta agtcaccatc ttcctctaaa gattccaaaa
    27841 gaaaaggaag caagttttct ggaagtttta aaatacgctt catctgcgat ttttcaatac
    27901 ttaagatatt aaaagttgta tcaaaatcag ccttcttcaa attagcaatt tcagccggag
    27961 ttaaacctaa agataaaatc aacaaagcaa tcaaacgacc actcggataa tctgtatctt
    28021 gatagaaatt tgataaatcc attatttgag actttgtctg agtcaaagtg attttttcag
    28081 tctcttgtag tctgtaaaat tccttcagcg ttcctctatt atacaaaaag aaaagaaatt
    28141 gattgactgc agataacttt cgcttacgag ctgttaactt aaattcactt agagactgtt
    28201 gatacaatgc cagtttttca gaatttacat gtccaccaat tattttataa aattgcttta
    28261 aatcatagtg ataggactta cgcgaattga gagataattt cttactctca ataaaattat
    28321 taatatcatt tatcattgtt tttaggagtg atagtatatt cgtctgtaaa atcatgtagg
    28381 aggctattaa tagcttttaa aatcgattta cgggttataa tgcctctaaa atcattttga
    28441 tcactgataa caggtaaaaa tggataatct acaatcttat gcataattgc tgtcagagta
    28501 gctgcctcat taatcggctc gatttttgta ttaaccatct caacaatatc agtctgtgcc
    28561 atctcccaat ctgttaattg acctttagat tggtaagcca taatgtctga gatactaata
    28621 gtacctacat attttttttc tttagtgata actggtacac gtgaaaaacc attacttgct
    28681 aatagtaaca taacgtggtc tgcattgtgc gtatccacaa aaatagcaac gtcttcagct
    28741 ggaattagat agtggtccaa atgacttagc aaaaacgact caaattcttt agcaatcatc
    28801 ttgaaaattc ctttgtcaag ttaggatagg gttggtgttg gcgagtataa tactctacca
    28861 aaaccttact atctgttata gttacaacag cgtaaagctt ctcatttatt ggtcctcttg
    28921 gctgcaaaac actacctgga ttgataaaaa tagttttacc atttttccaa gcatctgcac
    28981 gatgaagatg accgtataaa caaatatccg cgtcttcttg ctgagctaat aaatccaatt
    29041 tatcccaagt aaaattaatt tgatggagat gaccatgggt ttgaacaata acagtattat
    29101 ctatttttgt aaccaaaact tccggataac ctgaatcata atcacagttt cctgtgacca
    29161 catgaattcc ttcccatata ggatctgagc taggaagttc ggaatcccca ttgtgaaaaa
    29221 tagcatccac ttttcccaaa taatgattct ttatatcttt tacaatgtca cgatctccgt
    29281 gagaatcact cattacaact aattttctaa ttgccatttt ggaaatacct ccatcaattt
    29341 acggacagct tgcccacgat gtgatagatt atttttttct tgtgcgctca attcagctgc
    29401 tgtcctacta ccttctccga caataaagag cggatcataa ccaaagccat tttccccctt
    29461 aggttccgta ccaatataac ctggccactc tgcttcaact accaaacttt ctttatttgg
    29521 agctgacacg actaaggttg tatgaaactg tgctgaacgt ctttctttgt caaataccat
    29581 ggctaattca tgcaataatt tggcattatt acgagcatct gtcgcgtcag gacctgaaaa
    29641 acgagcagac catacccctg gcaaaccacc taaagcatct acttttaatc cggaatcatc
    29701 tgaaatcacc attttcccag ttaattttga aatagtctct gctttaagtc gagcattttc
    29761 ttcaaaggtc ataccagtct cttcaacttc tggtaaatct gggtaatcat ttaaattttc
    29821 aactttgagt cctaatttcc caaataattc tctaaattcc ttagtttttc cttcattgtg
    29881 agtcgcaatt aatatggtat ccccaaaatt attatcattc tttaaatcaa agaatttttc
    29941 taaagaagtg ccctctttag gtaagtgaac taagaacaac tgatcatctt cactaactaa
    30001 gatggcccct ttatgttgag tcactttgat gtgccttgaa tactcttgat taatcatatc
    30061 aacgaggaat gctaataatt gaaaatccga agtcatctgc acaacgtgaa gctgtagacc
    30121 ttcaactttc aaagttgata atagacgatg aaaaacatct tggatagctt cgtaagattc
    30181 atcattacca aaacaagtaa aataattaaa agcttggaaa ctacctaaaa accaattgcc
    30241 ttcagtttta ctttcaaaaa ttgtttttgt cataatacta tacgctccac atttatttct
    30301 tgactaagcc attgttctgc aatttcttta aaaccttttg ggctggcggt tgtgtaaaag
    30361 tgatgaccac cgtgtttatt ttgccaatta tggtttatct caaaatagtt caataaaaca
    30421 gaaatatcac gaacggtttc tgcgccacta tcaattaatt taacctcagc ccccataaca
    30481 ttttgaatga tgggacgtaa taagggataa tgcgtgcaac ctaaaattaa agtatctaat
    30541 ttaccaacta atggggacag cgtttcataa accacctttt tggctaaact agaagacatc
    30601 tgatttgatt ccacaattgg aacaaatttc ggacaagcaa gggataccac agcagtattt
    30661 ggagacaaag cttgaatttt ttgacgataa gcatctgatt taacagtcat gggagtacct
    30721 ataataccaa ctttccctga attagttgat ttgatagctg cgctagctcc tggtaaaata
    30781 acgcctaaaa caggtatgtc tagtttttct ttaatttctt gccaggcaac tgcagttgct
    30841 gtattacaag ctataacaat catcttaaca tttttagtca ataagaaatt aaccatctgc
    30901 caggtaaact ctctaatctg ttgagcaggt ctaggaccat acggagctct agcctgatct
    30961 ccaatgaaga ttacttcctc ttctggaagt tgacggaaca tttccttaac aaccgttaaa
    31021 ccacctacac cagaatctag aaagccaata ggcctactat ccatttttat tcctccaata
    31081 ttagtgacta aaagaggctg gacaacagtc ctagcctctc tatacaataa ccaatcagtc
    31141 aaatctacac ttgaccaagt ttgcccaaat cttgtctaga aatctgatgg actatcactc
    31201 actatttcat cactaaacgc ttcaatgcta aaacactctt attttttagt tttgatagct
    31261 gtttttgcgt gttttacaat attacggtat gtttgttgaa ctttagcttc actaggtttt
    31321 tgtcccattt gactcatcat ttcacgaata gcatccggtg ttaaacgagg gtgctctcca
    31381 atctcttttt caatttgttt acgagcaata aaaatgcctc ccacgagacc accaaacaaa
    31441 gcgacgataa ttaataaaat ccaaatagtt gttgacatct attcattctc ctcatttatt
    31501 tacatgactc attatagcaa aaaaagaaag agtttacaaa tatatgtaaa caaaatcaaa
    31561 gcctctatcg tccttgtcac gtcaatacac tattaaattt tcactagagc atgattaagc
    31621 acatagccga ttcctttatc ttatcctaag tgttttcccc aacttttgtg tcatccattt
    31681 ccaaatgaag agagctatat aaaaatctac caatccatgt agcagagagc ctagccctat
    31741 caaagaaaat agtgaccata aataagttga actactgtta gaagtcattg tgattatata
    31801 aacagtaata aattcgagca gaccatgaat tatgttaata gctacagcaa ataacaatgt
    31861 ttgccattta ctcatcaata aagagggctt tgacttaatc aagaaagccg caattaatgc
    31921 aaatccgata tgtgataaag ctctaagaac gattacaatc ggaaaaccag ctagtagaaa
    31981 tccaagtcct gtccctaaag ctaccaatat tgcaactgga acagatataa aaatacttaa
    32041 aaataaaggt acatggcttg ctaaggtgaa agaagcaggc ccaataatta ttttagcagg
    32101 catgattata ggaatcatga ttgcaaaagc tactaaaata gaagtataag aaaccatttg
    32161 tgttgttttt tttgacattt ttatctcctt tgaagctatt atacaaaata atcttacagg
    32221 tgtaaagaca cctgtaaggt tatttgtcta tgtataaaat tcctgctttt tttagagcct
    32281 cacatatttg gtcaaaaact gactgatctt gacaagaaat catatgtgta tgtaaaccat
    32341 ctgtcaaaga ggatagcaat tctgcattgg aaacttttaa tttagcagta aaattatcaa
    32401 tgtctttttg gctttgaatg tttaagggag cagtcagcat cccataaata gggtgctcta
    32461 cctccacatt cataatgatt ccttgatacc gcaaaataat tttcagttct tcttctgtct
    32521 gttctatgcc atgctgacaa accaatctag ctgtgaattg atgagtactt agagcactgg
    32581 acataaggta tccttttggg gtagaaataa tatcacattg ttgtgcacgc aataaggcaa
    32641 tatctccaac aatcacctgc ctactaacac taaaaatctt tgctaatgtg cttgccgata
    32701 tagcttcttt tgttcctttt aaagtcgtta aaatattttc tcgacgctcc tgtgccttca
    32761 tataacaaat tccttctttg gtgttatttc acaattaaaa actggctaga atatctaacc
    32821 agttagcctc aatcatttcg tgcaaaaaga cgaagaatat ttaagaatag attaataaaa
    32881 tcaagataaa ggcttagtgc catcgaaact gcccaaccat ctgcgacttg gccgccttgg
    32941 ctattgtaga catatttaat catttggtta tcataagcaa tcaatccaga aaagatgatg
    33001 acacatacga tactaatgat ataactcata cccccactac cgataaataa attaacaaga
    33061 cttgcaatta aaatcccaat taaagcagcc attaacgctt tacgcatacc tgataaatct
    33121 tttttagtct tagctccaat caaagccatt gcaaagaaaa cagctgcaga tgttataaaa
    33181 gcttgaagaa ctgttgtttg cgtgtacctt gcgataatga aacttaaagt aaacccattc
    33241 aaagctgaat aaatcaaaaa taatggtaat gcagctggtg tgtttcgacg cgccgctcca
    33301 ctagcaacaa agactaaaac caattctaag atgattgctc caaaatacaa ccctggcatt
    33361 ttttgcatga tggcaatcat attttgtgga aacatataca gcataatcgc agaaacagca
    33421 gctgacaaac ctactcctat ccctacaaga ccgtaaatct tagcaaaaaa ttgattcagc
    33481 cctgaatcag attgtgtgta aatgacgtta tcattcatat gtattttcct attctaaatt
    33541 ttaatgattg tgactaccta agaatttgcg actaaaacgg ataggtttct taaaaatacg
    33601 tttgagtgga cgagaagcct ctcgtactgc ccaatactta tcaaccatag tcacaatata
    33661 actttcttta taacgtggtg gtgcaatagt cgctccccac atgtgtttca aaataatatc
    33721 ttcttctcta gcgtttaaat cagttaattt tcgcgcatta cgaaccgcta tccttggatg
    33781 aacccaagca tggctcttgt taaatttcgt tacacgccaa tcgtaataaa agaaatcatg
    33841 tagtaaacca cctcgagcag tactactcgc atcccaacca aacttcttag caattttata
    33901 acttgtataa ctaacattta tagaatgctc taagcgagtt gagtgatgat gttggacaat
    33961 agcttcgagt ttttggaaac gtggatggtc aataaggtga ccgaccaaat ccatgaagtc
    34021 cttatcgtct tgatactttt tcattataat cacccctctg gggatattat aacatatgaa
    34081 cttaaattaa gctgaaaatg actactcccg cagctactgc tacattaaga ctttctgctt
    34141 gtccaggcat ttcaatatgt gctagaacat ctgcttcttc tgtcatagtt ttggaaattc
    34201 cctgtccctc atttcccatg attaaagcaa aatgatcata tttagggaga tttttataat
    34261 ttaccgattg ttcagataaa gttgtcgcaa tcatagttat cccttctgct ttacaaaagt
    34321 taacaaactg ctctatttct accctaaata ctggtaaatg aaagtgactg ccttgcatag
    34381 aacgaagtgt tttttggtta tacaaatcag cagatttctg agataaaaaa acagcatcaa
    34441 aattcgctgc atctgcagtt cgaaccattg tccctaggtt tcctggatcc tgcacatctt
    34501 ccaatacaag aaaacgccct tttttaatat ctgtccatct tgtctcttga aaggcaatct
    34561 cagctacaat cccttgagga gtctgtgagt cagttaattc tttcaaaaca tcatctgtaa
    34621 caacaatagc ttcctcgggt tttctcaaac gatctattgc cgtttctgtc acaaaaatat
    34681 ttaaaaattg tgcaccatat ttttcagctt cctcaaataa atgccagcct tcaataagat
    34741 atgaagattt ccgatatttt ttttgtaaca actttttcgt ttttttaatg agattatttg
    34801 attttgaagt tataatagta gtcataaacc tattatatca gaaaagaggt caagttatga
    34861 aaaaagttca tttaattgtc tcagggcgcg ttcaaggagt cggatttcgc tatgcaactt
    34921 attccttagc tttagaaata ggtgatattt acggtcgcgt ttggaataac gatgatggaa
    34981 ctgttgaaat tttagcacaa tccacagatt caaataaaat gacacaattt attcagaaaa
    35041 ttcgaaaagg accttcaaaa tggtctaaag taacttatgt tgatattaaa ctagataact
    35101 tcgatgattt taacgacttt aaaatgagga attaaaaatc attctgcaga agtattgttt
    35161 aattcttaaa aaaaaagtaa aatagatagg tatattaaaa attagaggaa ctaaaaacat
    35221 tgaaaaaaac attgaaacgt atcttattct ctagcttatc tttgtctata ttattattat
    35281 tgacaggatg tgttagcgtt gataaggctg gaaaacccta tggagttatc tggaatactc
    35341 ttggagtgcc tatggccaac ctcattactt atttcgctca acaccaaggg cttggttttg
    35401 gtgtcgctat cattattgta accatcatcg tacgtgtcat tatcttacca cttggacttt
    35461 atcagtcttg gaaagctagc tatcaagctg aaaaaatggc gtactttaag cctttattcg
    35521 aacctatcaa tgaacgacta cgcaatgcta aaacacaaga agaaaaatta gcagctcaga
    35581 cagaattaat gacagctcaa cgtgagaatg gccttagcat gtttggcggt attggctgct
    35641 tgccattact cattcagatg ccatttttct cagctatttt ctttgctgca cgctatactc
    35701 ctggagtctc tagtgcaacc ttcttgggac ttaacttagg tcaaaagagt ctgacactta
    35761 ctgtcattat cgccattctt tattttgtac aatcttggtt gtcaatgcaa ggtgttccag
    35821 atgaacaacg tcagcaaatg aaaacaatga tgtatgtgat gccaatagcg atggtattca
    35881 tgtcaattag tttacctgct agtgttgcgc tttattggtt tattggtggt attttcagta
    35941 ttatccagca gctagttacc acttatgtac ttaagcctaa actacgccga aaagtagaag
    36001 aagaatatac taaaaatcct cctaaagcct acaaatctaa taacgcacga aaagatgtta
    36061 caagttcaac taaaaccact gaatctaatc aagcaattat tacatctaaa aaaacaaatc
    36121 gtaacgccgg aaaacaaaaa agacggggat aaaacaagca tactaaataa tggtgtgttg
    36181 tcctaaacag ttaaagatta ttatgtaatt caagtaaaaa agacttagct atgtagaaca
    36241 tagactaagt ctttttagtt ttgcctaact tttcatataa aaagcaccct aaaagtcaga
    36301 taaaaatcta gcttttaaag tgcaagtaat ctagctaaca gactacctta ttaataaagt
    36361 aatcgttttt cccaccagtc ttgaataatt gcataaagcg tacaaattac ccaatagata
    36421 agggctacta agatataggc ggtcatatag tcggagtagg ttcccccaac tattttagca
    36481 ttattaaaga tatcaggtac cgtaatcatc gcagtcaatg atgaaccctt gaccatatct
    36541 agcaaaacat tacttaaaga agggagtgca attcttgttg cttgcggaat aataatgcct
    36601 ctataaatcg tcggtgtttt taaccctaaa gctctagcag cttcccactg accatgatca
    36661 acagctaaaa gagctgctct gataatttct gatatatatg cactagacat catggtaaaa
    36721 cctacgatag aagcagtcac agcatctaac tgtatcccta taaaaggcaa accaaagtag
    36781 ataagaaaga gtagaaccat taaaggaatc ccacgcatta aggaaatatg aatattagct
    36841 agatacctca gtaagcggag atgagacatc ttcattagag ctacaaagaa tcctacgata
    36901 gctcctaaaa taaaaccaac caaggataat ccaatggtat atggtaaccc ctgaataaca
    36961 cttggaaaag ccttaacagc tagttccaaa ttaaaaatgg catcccagtt tatcacttat
    37021 ctactcctct cgaaatattt tatttaactt taattactgg aattttatct gttccagcag
    37081 gttcttttgt gagatcttga ccagcataat atttttcaga gatttttttc aaagaaccgt
    37141 ctttcttcat atccttgata gctgcatcaa tttttgtttt gaggctctta tcttttttac
    37201 tcatcacaat accttgctca gtcggattat atttcacatc acccattttt actttgatat
    37261 ctggatattg ttttgttaca tattttacag caatgacttg tgtgtagtaa tcattcggaa
    37321 taaaatctgt acgtccagtt gatacatctc gaaggtaaac gtcattagtt acgttgtcat
    37381 aaataacagg ttcagcgcct tgctgttttg caattttcat atattgcgtt ccagcgccac
    37441 caccagcttt tttacctttc cagtcagata aatcttttgc tgtgatcttt gaagagccat
    37501 ctgcacgtac aatcatacct ccaacagaat atttataagg ttgagagaaa ttgtattttt
    37561 tcaaacgctc gggagttgtg tcgaagttat tgacagcaac atccaccttg ccactatcta
    37621 cagaagtaaa tgattcagca actccaactt caacaaactt aacttttatc ttaagttttt
    37681 tagcgatcgc tttcatcata tcaatctcat aaccagtcaa ttttttatgg tcatcataat
    37741 aacttgttgg ataaagcgtt gcaggggtag caaccttaag gacaccatct tttttaatat
    37801 tgtcccatgt tgtttctttg ctagattgac catttgagca agctgctaat gtcattgcag
    37861 ataaaccgac taaaccaagt aaaattgtct ttttcataag tactcctgta gattaaatta
    37921 tattaaaatg gtttggcgta ctctatcgaa aattaaattt aatgaaaatt aatacataaa
    37981 tttacgataa taatacaacc attgttaaga gtaccatatt gtatccgttt tcgcaaaaat
    38041 attgtttact tttcaaatta atggcaaaca ggccctttga attgcttctc tttttcaaac
    38101 aactctgcaa cagcaagcaa taaatcttct ctccctttag ccgctgtcaa ttgtacacct
    38161 aaaggtagcc cctctttagt tagatgagtt ggtatggcta aagaaggttg cccagtcaag
    38221 tttggctgat gtgtaaaagg tgtccaggcc attgagtctt caaacatttt ccaaattaaa
    38281 tcctgttgtt cacttactga gaattctctc atatgcctca attgtttctg gagtgtctcg
    38341 tctaaatcaa attgtccatg aaaaggagct ggttgattag ttgctgctgt taaaattaaa
    38401 tcataatttt catgaaacct cgccatggtg gctgcaaatt gatcccatga atctaagagt
    38461 ttactgtaat ctttagccaa taccctttgt ccactctgat acatagccca ggtcattaat
    38521 tccatatcgc taaattccat tgaacgccct aaactctttt cgatgccatc aaacattgct
    38581 gctgtttcaa cgctattcat catacaataa gttttcatag aatgaatacc atctaaaggt
    38641 tgttctacta attctaaatg attccctttt tgagacagaa aatctgcggc ttctttaata
    38701 gctgctttag cctcacttga tacctttgtt tttaagggag aatccatcaa tactgctatt
    38761 tttaggggtt tactaacgct aaactcgaaa agactctctt tgctgagttt tttcagggga
    38821 aatggacttt ctacttgata tgattgtaaa taatataata gtcgttttgt atctctaact
    38881 gactttgtta aagcaaaatg actagaagcc ccttgccaac ctctatagga tgaaggtcca
    38941 acaggtatac gtcctcgact cggttttaaa ccaatcaaac cattaaaact tgctggaatt
    39001 ctgattgacc ctcctccatc actagctcct gcaattggaa ccattcctga actaacagct
    39061 gcggctgcac caccactaga gcctccagca tttcttgaat ggtcaaatgg aagattaaca
    39121 ttcccgtgaa gttgactatc actaatattc ttaaaaccaa attcaggggt atttgttctt
    39181 cctaaaataa taaaccctaa tttttcaaag gactgcacta aatagtctgt ttgtttagca
    39241 taatagtgtt taaataattg tgaacctgag gttgagagct gtcctttttg attctgtccc
    39301 aaatctttca gaagaatagg aactcctgca aaaggagcat ttgatgcatt tgcgtatttg
    39361 gctgcttgac gcgcctcatt atattgcttg ctgacaacag cattaacgct tacattctgt
    39421 tcctcgatct tgtagattgc ttgctctact aattcttgcg acgaaacctt atgctgtttt
    39481 atcgcttgtg ccatagcggt agcatcttta aaaaccatat aaacacctca taaaatgtat
    39541 ttttattata agataacaaa aaatacttaa aaaaaataga ataacgtcta gactctagat
    39601 attattctat gatttatact attaatttgt tttttcaaca ctaatgattt caacttgata
    39661 gctaccagca ggtgattcaa ttgtagctaa gtctcctgtt tttttaccaa ttaaagcatg
    39721 agcaattggg ctttcattcg aaatttttcc tgaaaaaatg tcagctccag cagcaccaac
    39781 gatatggtaa gtatctttat catttgtacc aacttcttgg acaaggacag ttttaccaat
    39841 tgcaacctca tctttagcaa ctgcatcact atcaataatt tcagcataac gaatttttgt
    39901 ttctaaaatt tgaatttggc cctcaacaaa tgcttgttcg tcttttgctg catcatattc
    39961 gctattttct gataagtccc cataagaacg agcaatcttg atacgctcta caacttctgg
    40021 tctacgaact aattttaact cttcaagttc tttttcaagt tgatcttttt ccacttgtgt
    40081 cattgggtaa gttttttctg ccattttatt tctcttttct tttcattttt tcaagtcaaa
    40141 gatataaaca tgatgactcg caccatgttt ataagcttag ttaatttgac tattgatata
    40201 tttttcaacg ttagccttgt gtgtttcaaa gtctttagcg tagtatacat ttccggtctt
    40261 cacatcagcg acaaaataaa gataatctgt tgaagcaggt tttatagtcg cttcaattgc
    40321 tgagatactt ggactatcaa ctggtccagg catcaaacca gtattagtat agatgttgta
    40381 tggtgattta attgaagtat taatttgagc atcttcagct aaacttgttt tatcacctaa
    40441 tttccccata gcatacaaaa ttgcaatgtt actttgaaga gcttgaccgg ctgataatcg
    40501 gttataaaag acacttgcta ttttacggcg atcttcgtct gttgatccct ctttttcaac
    40561 aagagatgat aatgtcaaaa catcatttac agacatattc ttcgctttaa tagtgttata
    40621 gtaaggtgcc atcttagtat tcattgttga aatcatatct tcaacaagtc cttctaaggt
    40681 tgtatcttta taatagttgt aagtagctgg gaataaatat ccttccaatt gataaatagc
    40741 ttttgattta tcaggtagac ttcctagtaa atttggatat ttagctacca tttttttaat
    40801 aaacgtttca tcttgaacaa gtttcaagaa atcatcagct ttataaggtg tacttgtagt
    40861 ctttgtatca attttattag actcaatagc tttagcaatc tgtttaatag tgtaaccctc
    40921 ggtaattaat atcttaccta aagctggttt tgtcggttct gcagttccgc ctttttccaa
    40981 ttcagcagca atctgatcta aagtcatact ttttttaaga ttgtagtatc cactttgaaa
    41041 attactatag tttttgaact tggaatagta attaaagact gtggcgctct taatgactcc
    41101 tgctttttct aaaatttgac caattaattt attcccagat cctatcggaa tttcaacttg
    41161 tacaaattta tttgaattgg aatcaacagg tttaatagca ctatctacaa aacgataacc
    41221 tacaaatgct gtcaagagca atacaagtat gataattgat atcagaatac gtgcaattcg
    41281 ttttgccaca ttatctgctt tctgacgtct ttcacgcttg ttatgtttgt tcttggctga
    41341 caaagctata tcctcttctt cttttgacaa atcctctact cggataggtt cctttgctac
    41401 gggctctaac tctgatgtct caccaagttt tacattttca ccttctgtag gaatatctgt
    41461 tgctctcaca agaaagattt cttccgtttc ttgattaggc acagcagttg caacatcagt
    41521 cttatctgct tttaaatcta tgtcaggtac attttcagtt gtcctaaatt cactagattt
    41581 ggagccgcta tcaagctctc ttgaaggttc catcccttga ttgataactt gttcttcttg
    41641 ggaagctaat gcttgttccg cctgacgtct ttcttcttct cgagcagctt ctgccagaag
    41701 tttcgcctca atccgagcct tctcttgctc ttctaagcgt tgcttagtct ctagcgcctt
    41761 tgctctagct tcttgttcgt ctttcaaacg ttgcgcttca taatctgcca tcaattgtgc
    41821 tgttctgcga gcagcttctt tggcttcttg ctctttttga tagagttctt cttcacgcaa
    41881 ctttctcaaa cggttagctt cttctaactc tgccagaatt tgttctttaa aacttttctg
    41941 atcatggtta gaatgctgat cgtcattaaa ttcggtcaaa gagatttcct cctaataata
    42001 atcacaggca attatatctt aaatagtcta taaaatcaac aaaactttga caatgttatc
    42061 tacttttaac aaattgatcg tgttggatta ttccaaatca tattatacca aagcgaacca
    42121 ccatgttggg agtcagattc tccctcatct ttaaatccat tcatttcata gaaggaaatt
    42181 aaatcatcat ggcaagttaa acttatacca tctcgttctt gagaaacaac caggtctttc
    42241 atcgctgcta ataatgctgt accaatcccc tgacctttaa aatcaggatg tattgataga
    42301 ctagtaattc ctataaaacc accttccctt acagggattt cagaaacttt atggaataaa
    42361 tcgtccgtta agtaccgacc tttaataacc ggtccttcaa tgtaacctgc taaacgacca
    42421 ttaatctctg caactaaaaa agtatcagtc attatcatta aacgttcttg cattgctttt
    42481 ttactcgcag cctctgcttc tgaaaaattt attcgttcaa tactaatgac ttgctgtaaa
    42541 tcctctaggc aaccatttct aataatcata ctcatctcct taatttcttt atgaaattct
    42601 cttgataaag tcagatagag gactgagaaa cttctctcag cccttactat caacttattg
    42661 tgtattttta gttaggttag ctaataattc ttcaaaagag cgctcataca attgaatgtc
    42721 tccagcaccc ataaagacat agacagcatt atcatgattg agtaaaggcg agacattttc
    42781 gactgtcact aaatctgagt gtttgacaat cttagcagct aaatcttcta ccttcacctc
    42841 accattatct acttctctag cagaaccata tatttgagca agataaacgc tatccgcttg
    42901 actcaaagca tgggcaaaat cgtctaaaag agctatcgta cgagtgaacg tatgcggttg
    42961 gaaaatagct acaatttctt ttgacgggta tttttgtcga gcagcatcta atgtcgcaat
    43021 aatctcagta ggatggtgag caaagtcatc aataatgaca gtatcgtcaa taatcttctc
    43081 agtaaaacga cgttttaccc ctgaaaatgt cttcaaatgc tcagctacta atgccatatc
    43141 aattcccatt atgtaaaggt tagcaataac agcagttgca tttaagatat tatgtttacc
    43201 gtatgctggt acatgaaact gaccaatttc ttcttggtta tagaaaacct taaagtcaga
    43261 accattaaca gttcgagtga tgtcttttgc tataaaatca tttgaatctt caaaaccata
    43321 ataatatatt ggtgccttag aagtgatttc atgaagtttt gaatcttctc cataaatgaa
    43381 taaacctttt tgaacttgct tagcatagtc attaaaagca ttgaatacgt cctctaggcc
    43441 tgtaaaataa tcaggatggt caaaatcaat attggtaata attgagtatt ctggatggta
    43501 cggcataaaa tgacgttcgt attcatcagc ttcaaacaca aagtaattag cattagcaga
    43561 accacgtcct gtaccatctc caattaggaa agaagtgtct gtaatatttt ttaaaacatg
    43621 agctaataaa cctgtcgttg aggtttttcc atgtgcccca gctacaccta gactagtgaa
    43681 ctgacgcata aaatctccga gaaattcatg atatcgttta aaatgatagc ccttttcaat
    43741 aacataagcc aactcttcat tgttatctgg acgaaaagca tttcctgcaa taatctctaa
    43801 atcctcactg atattattcg gtgagaaagg taatatagtt acacctgctt gctctaaacc
    43861 acgttgggta aaataatatt tgtcaacgtc acttccttgg acgttatgtc ccatttgatg
    43921 aagcatcagt gctagggcac tcattccgga tcctttaata ccaataaaat gataagtttt
    43981 tgacatggtc tctccttaat taatgtcttt aaaacgtgtc agatttaatt cttgagcctt
    44041 ggctctttct ttgtgaaact gattgtcttg tttattgtaa acttgactgc gtttcaaaaa
    44101 atcaaaatta tttttctttg tttttgggtt cggtgtatta tcaggttctt gaaatatgat
    44161 tggaatttct gctaatatat agctttcctg ctgtaaacga tcagaaagat gacttagttc
    44221 agtcaccttt cgctgagttt gttttttcgg tactgttgcc ttcaattgtt gctgaaaagc
    44281 ttcctttgat ggcgccttaa attcctgttt cgcatagtaa gcttgtcgtt tctcccgaac
    44341 agacctcttt gcctcttgtc gagatttgct caactctgtc gcttttttta caaaactctg
    44401 cggtggtaca ggatcatttt caattacctt aggatgtgat atttcttcat gttcataatt
    44461 gcttttgacc atgctttgat atgttttttc ttgataaaag cctcgaatgt tgttaattaa
    44521 atcttcatta tcataaagat tcatctttac aagcggagac attactggtt catcatctgc
    44581 tatcaacgga aatcgtctag agcctctatt cacattatca cttcctttct acaacagacc
    44641 tattataaac taatttttct gttttttcaa agttttcaac taagtaaagc ttatcagatt
    44701 ggtatttttc attcatttat ataaaaaaga aagcgagaga ccactttctt ttttatatta
    44761 tagtcacatt atcatattta tttagaaact ccaagtatct ctcgaatatc atctacactc
    44821 atgctagcat gggtttcatt tccatccaga actgttgtaa ccaaatgttt tttagtttct
    44881 tgcatttcta gaattttttc ttcgattgtg cctctagtaa tcaagcgata aacctcgaca
    44941 ttctcttttt gccccaacct atgtgcgcga ctaatcgcct gcatttcaac agctggattc
    45001 caccacaagt caatcagaac aacggtatca gctcctgtaa ggttgagacc aacgccacca
    45061 gcttttaaag aaatcaagaa agcatctttt gatcctgcat taaatgctct ggtcatttca
    45121 tgacgttcat ttgctggtgt agaaccagta attttataag ttgtaagccc catagctacc
    45181 atctcacgtt ctgcgatatc taacataccc ctgaactgag aaaaaatcag agcacgatgt
    45241 ccattttcct taatttgtgt taaaagttga tgtaggcttt ctaacttacc gctttcacca
    45301 tcataatcca taaaaagacg tggagtgtcg caaatttgac gtaatcgtgt gatgccagat
    45361 aaaatttcaa ttttacgacg atttaagtca gcgtcactag aatgttggat actttcttga
    45421 atttggcgta attgcgccaa atagataact ttttgactgt ctgtcatttc atttggatag
    45481 ttcatttcaa tcaaatccgg taattctggg agaacttctt cttttcgacg tctcatcaca
    45541 aatggtttga tataacgagc aacttgttta gggttcaact tcaaaaactc tttcttacca
    45601 ggtaataaac caggtaaaat gatctggaaa atggaccaaa tttctaacag tttattctca
    45661 ataggtgttc ccgataatgc aaaacagttt ttgacctcaa aagatctcaa gctatgggct
    45721 attttagttt gagcattttt cataacctgt gcttcatcca aaattaaata atcataattt
    45781 ccttcagaat aagtctcaaa atcctgccta aatgatgaat aacttgtaat gattacttga
    45841 tgacgttcag caataatttc atctctacga atcttagaac cataggcaac tgctacatct
    45901 acgtctggcg caaatttgtg gaattcatct tgccaattat aaattaaact tgatggtgat
    45961 aaaataagca cacgagaatc tctagttagt tttgtactta aaaatgaaat cgtttgaagt
    46021 gtttttccaa gccccatatc atcagctaaa atccctgcaa agccataatg atctaacata
    46081 cttaaccaac ggacacctgt aagctgataa tcgcgcaagg aagctgaaac agatagacca
    46141 ggtattttaa acttttctgg gtgtctcaaa tcttcaatca attggtaaaa atgttgtgaa
    46201 aagctaacat tatcttgatt tgcaaacagt tctgataatt gaaatgctgc aatattatct
    46261 aattcaatat gcccattctt tgctcgtcta gctctcaacc cttgcaaagt tgcacttact
    46321 ttcttagtct cttcatcaaa aataactaat tgccctgatt tattcacaaa ataaggatta
    46381 ttgtcaaaca aagctaccag agcttgatca acatcgtttt ccagtaaatc agagaaatca
    46441 aaagaaatat ctagcaggct tcccttagct tcaatatcaa tttgcggtcg ttctatgaga
    46501 cgataacttt ccaattttgc ggataagctc actgttccca tttttttaaa ttgtggaagc
    46561 atataggtat aaaaatcgta taattcttgt gcactgtatg ctggatggct ggttgaaaag
    46621 tggggtgcaa agccatatct ttctaagagt ttaaatattt tttcttcatg tttgaagtga
    46681 ctagcaaatg ttaactgttc tagttcttga cgattatgaa cagttaaatc attaccataa
    46741 tcaaatacca tttgcaataa aatttcattt tgactattaa tatcgaactc aaaatttacc
    46801 ttaaaatcat gaatagagaa gctccgtgga gcatctacta gtccaatctc cttaaaatcc
    46861 agtaaatgag ctgctaactt atcttgatca tctaaatcaa aatggatatg ttttgctaaa
    46921 tctccctcaa tgggaagact acgaatagcc gttaccatct tttcttgctt caaattaaga
    46981 tggtagaaag tatcgcgata aaacaaataa gaattcgcaa atagtggttt taagtctttc
    47041 tctttgattt ctaattcaat tgattgccta tggacaataa ccttaaattg ataaagatga
    47101 tcctcacctt ctaattcctt aaagtagagg tgattaaatt gatgaaaatc actttcaaat
    47161 gaaaaatttt ctaaagcatt catcaaagta acgccctctt caaaaaaacc acgagtgaga
    47221 ctaaggtgac gaccttgatt tggtaaaata aactctagat caattttgct tgaatgactt
    47281 ggaatgagcc tccataaaaa ttctatcaat tcttgactgg tctcatcaaa ctgtatcaaa
    47341 gacaaagttt caaagtattg ttttccaatc tgataataag cttcttttcg aacagtattt
    47401 aaaaaagctt tgatgtcacg aataacataa cttctatcat caggcagtcg tctaattttt
    47461 aaagtccacc agatatcatt agcataagga ttttgttctc cttgagccga tagttgatat
    47521 tttatagtat catcttcatt aataattaga ctatctaaaa aaacactacc aaaagaggtt
    47581 ttcttcttag tttcttgttg actttcttgt tgaacttgaa ctttagataa gattgcttta
    47641 ccctctgggt cgtttttcaa gaaatgctcc agagcagcta aatgttgaca atagcctttt
    47701 cttgcaaaaa aatcacaaga acattttgtt tcttctgtta ctaatgaata ttcaatctga
    47761 caatccccaa ctttagcttt taatagatta ccttcttgag aaatcaagga caccaaacct
    47821 tgttcgtaga gttctatacc ttgatttcga atacgtcctg gaatcattct actcattatg
    47881 ctaccacctt catcttatcc ctcaattata acaaaattca agtccaattt cttgtaaaaa
    47941 tactaaattg attgtttttt gatacagtaa caaataaaaa aaggctaaaa caactgtcct
    48001 aaccttctat ttcaatttca acaataagat gttatttacg tttacgtgct attagattaa
    48061 ttggtgttcc ttcaaaaaca aatgcttcac ggatttgatt ttctaagaaa cgaagatatg
    48121 aaaagtgcat caattcttct tcgttaacaa aaacaacgaa agttggtggt ttaacagcaa
    48181 cttgagttgc gtagaaaatc ttaagacgtt ttcctttatc agttggtgtt ggattaatag
    48241 caatagcatc cataatcaca tcatttaata cagcagacgg aatacgtttg ttttgacttt
    48301 cgctaatacg tttaatcata tctggtagct tgtgcaaacg ttgttttgtc tctgcagaaa
    48361 caaagataat tggcgcataa gacaagaatt ggaagttatc tcgtatatca gcttcccact
    48421 gactgacagt atgattgtct ttttcgatag tatcccattt attaactaca ataataatac
    48481 ctttaccagc ttcatgtgca aaccctgcaa tacgcttgtc atactcacga ataccttcct
    48541 ctgcattaat caccataaga acaacatctg agcggtcgat ggcgcgcata gaacgcataa
    48601 cagaatattt ctctgtgttt tcatatacct taccagattt acgcatacca gcagtatcaa
    48661 tcatagtata ctcttgcccc tggctatcaa cgaaattagt atcaatagca tcgcgcgtag
    48721 taccagcaac tgggctagca ataacacggt cttccccaag gatggcatta attaaactag
    48781 atttcccaac atttggacgt ccaatcaaac taaaacgaat aatatctgga ttttcatttt
    48841 cctcttctac cggaagattt tccacaatag catccaatat atcaccagta ccaataccat
    48901 gaactgatga caagggataa ggatccccta aaccaagaga gtagaaatcg taaatatcat
    48961 tacgcatctc aggattatct accttattaa cagctaaaat aaccggttta ttggtcttat
    49021 aaagaatacg tgagacatac tcatctgcat cagtcacacc ttctttaccc gacacaacaa
    49081 acacaataac atcagcctct gtcatagcaa tgtctgcttg gtgcttaatt tgctccataa
    49141 acggtgcatc aacatcatca atcccaccag tatcaattaa tgaaaactta cgatttagcc
    49201 actcacccgt tgtatagata cgatcacgag tgactccttc aacgtcctca acaatggaaa
    49261 tacgttctcc tgcaattctg ttaaataatg ttgattttcc aacattcgga cgaccaacaa
    49321 tagcaactgt aggtaatacc ataattctcc tctcactatt gggaatactc ccaattccat
    49381 tctattttat taacggcgat tcggtccttc taagtggaat tctatcgcta aataccgtac
    49441 gcgctccata actcgtttcg cttgccaagt ttcgtcgctt ccttttatat tagcccattt
    49501 tctttccaaa tcgttaaaac tataatttga cgtaaagaaa gtgggcaact cttctagcat
    49561 acgatgttgt aatataactt gtaaaatctc gtcacgaacc caagacgttg cctgttcagc
    49621 cccaatatca tctaaaatca aaattggaac acttttcaca gcatcaattt catcttttac
    49681 agttccgctt gaaatagcat ttttgacatc aatagcaaaa ctagggaaat gaagaagagt
    49741 cgttgacaca ccctttctct ctgataattc cctcgccatt gctgccatta aataggattt
    49801 cccaaccccc atatctccat ataaataaag ccccttatga ttatatgaag gataagtctc
    49861 tacaaaatca agcagttgtg acatagcttt cattcgactc tcgttattaa tatcaaaatc
    49921 agtcattctg atattccgat aggacttggg tagattaact aaattaatac gatcactgat
    49981 agcttgtttt ttttgtgctt caattaactc tcgtgtttct aaataagaaa catctgcata
    50041 accttcattc ataaccaaaa taggctcata gcctttagca atatattgac tatccttatt
    50101 tttaaatttc tgacgctcaa tcaaaaattg attaaattta gacatgctga tatttatttc
    50161 acgctgagaa agttggtggg ttttaatgaa ctccgctacc tgagcatctg ataaaatcat
    50221 ttgaataagc tcatcatttg tattgcgagg cactctccct tgattttcta atgcttgtcc
    50281 cactgatttc atgattcacc atcctttcct aatcgttcta aacgcttgag ggcatccgtt
    50341 ttaaactgtt ctaattcaat ttctttttct gggctaacct catctttata atcaggatta
    50401 ctccattcag gaacattcga ttgtttcttc ttcgtttctg ttttagtcct tactttttga
    50461 tctgaaaaat cacgaatttt aagcacagca tcttcggccg tcataacatt ctgaaaggca
    50521 aaatcattag caactttcat gatataggcc ttatttaagt tagcagattt tgtcttattc
    50581 aaggtatata aaaccataac attaatgact tcgtctagga aattcatttt tgccaaatcc
    50641 tcaagtaaag tcttctcacc acttgttgtc actgccttac ggcttctctt gattttttca
    50701 aggaacacta gagcactatc atttttagat tcccttagta tgaccttttc attatttgta
    50761 aaactctgat tatctttaat atgctgcccc tcattttgtt gaactttcat tcgctgaggc
    50821 gcaatcattc cattgatagc tgtttgtttt gctaattgat aggtatcata ccaatttaaa
    50881 tgataaagtt ctgatacact ataaatacct aagacatcat ctttttcatt gttaaagcgt
    50941 aagccatctc gcatcatcaa acgtttgaaa ctttccaagt caaaaacatt tttactccgg
    51001 tttacctctt gttttggttg tcctaaatcc gagaaaacat ctgtaaattt tttagagata
    51061 tctctagcat ttttaggtat tttcatatct aattctgcca ctgctacctc tcctatacgt
    51121 ttttctaaca aacgacgata gatgggatta gataaaaata actcatttga catagcttga
    51181 ttcaacttaa caaggtaagt acctgtggct tggtaaacag tcaacaaatc caaagctgtt
    51241 aacattacca atgcatcttg aaatcttggc atcccgtatt gcagatgatt gagaacctct
    51301 gaaaatttat gacgtcgtat tccgtcatca aaaaaatgaa caaaataatc atagagtgcc
    51361 atggcatcac ttcccataat tgggaaatac aattgtatta aactattact atcataaaca
    51421 atcttatttt gcttaatata tgtaaactca tctattggac tcatcacacg ctaccactct
    51481 ttttactccg tacgcgcttt gtaatttgtt gaagtagttc ttcaatttcg tctacatctt
    51541 taaaagattt ataaacacta gcaaagcgca cataagtaat ctcatctaat tcggcgagtt
    51601 catccatcac taaatttccg atagtgatac tagaaacctc atcttcatac tcactcctaa
    51661 ttttacgttc gatacgtaaa atacaatttt caatatcttc gctcgaaaca gggcgttttt
    51721 gagcactctg aataatacca ttcaaaattt tatctcgaga aaattgctcg cgagtaccat
    51781 ctttctttat gactaaaaga ggcaattctt ccaaacgttc aaatgttgta aagcggttgc
    51841 cacatttttc gcactctctc cggcgtctaa tagtcgtacc ttcttcagct tgtcggctat
    51901 caaccacact agatttatta tagccacatt ttggacaacg catgtcaaca cccctattct
    51961 atcatatcat ttaatcatct tattttaaca caaattcagt aataacaaaa gaagcgcctc
    52021 tcttatcacc tcatcatatc aaaacacaaa ataatcctta tgccttttat cattaagcat
    52081 aaggattttt cttatatttc tttagtttct tcaaattttt ttataggaat gcgaacaatg
    52141 aattcagtcc cttgatttag ttcactttta atatccacct ttaaatggta agcatccata
    52201 atttgtttaa acactgagag cccgatacct aaaccagctt gtgtactttc tctgttacgt
    52261 gatttatcag ttcgataaaa acgatcaaag ataaactcaa tatcttcatc tgagatacct
    52321 tctcctttat cttttacaac tactgtcgca aaatcattgt ctactgataa aacaacactg
    52381 acttctttat aactaggaga atacttgata gcgttatcaa ttaagatcat gagagcttgc
    52441 tcaaaatgta tcttatatat ctttccccaa acgatatctg aaatattatt ttcaaaaata
    52501 aattgaaaat cttctctcaa aatacggaaa ttttcaataa caactgatat agagcttgat
    52561 aaatctgtca cttcatcttg atgaagttcc aagcttcctt ggacacggat catatttaac
    52621 atgtcattaa tcattaagga catacgatct gcctcatggt atgccgctgc taaactttct
    52681 tcaagaattt ctggatcatc tttcccccaa cgttgtaata aaccaatatg accctttaca
    52741 acagcgacag gcgttcttaa ttcatgacta acatcactaa tgaattgtga ttgtcgacga
    52801 gtatagtctt ccaactgatc caacatccca tcaaaaatcc tagacaattc tccaatttcg
    52861 tcttctgaac ggaccttact tctaatttct aagttactag gattttcaga tatctgatgc
    52921 attaagtcgt gtaaattttt aaccggacga acaatacgct tagtagcact attaataacg
    52981 accaaggcta aaacagtccc taaaacttcc atcataatca agaaaacaat gatgttttgc
    53041 ctcatactat aataacgacc taaattatag aaaatttgaa cgtatccaag caactgtcct
    53101 gttttttgag agtagattgc ttttgtacca acaaaacctt ccatatctgc aactttagag
    53161 atgataactt ttcccatgga tttaccaata gttggcatac ccgttctcag tgtagaaaaa
    53221 actggtttcc tgttgacatc gtatagggtc atttcttgat taacataaag aatactatcg
    53281 attgactcgc ccgttctaac aattccattt tgacgcttat gaggatagat gtcatccgca
    53341 aaaacctggt tgtacaggat ctctaaaata tcatcggatg tcaaactaga gttcgcttgc
    53401 gacaaacgaa ctctagtttt ttctaatgac ctaccgacat taattttttc cctgtgaagg
    53461 acgtatcttg ttgccgaagt ataaactaaa acagaaaata ctgtaaatag gctaaaaaat
    53521 aagataaaaa ctaattgcga taattttttt gacagaggtt gttttacccc tataaactga
    53581 tcttttttat ttttcacgaa tcacatagcc cattccgcga acagtttgaa tatatgattc
    53641 acgacctggg atatcaattt tacctcttag gtaacggata taaacatcaa caacgtttgt
    53701 ctctgctgcc acatcgtatt tccaaacatg ttctagcaac tcttctcgtg tcataacacg
    53761 attcatattt gtcatcaaga cattcaacaa atcaaattca cgttttgtta gtgaaatttc
    53821 ctcatcacca cgcatcgctg aacgattatg agtatttaat gacaaatcac ggaaactacc
    53881 actatcacct ttttcttttg tcttcgtttc aatttcttgg cgtcggaaaa tcgctctaac
    53941 acgtgctaat aattcttcga ttgcaaacgg cttaacaata taatcatctg ctccacgatc
    54001 aagaccagct acaatatcca taacagaatc acgtgctgtc atcatcatga tataggttgt
    54061 tttttcagcc tgcaggcgac gtgtgatttc gaaaccatcc atctctggaa gcattaaatc
    54121 cagtagaatc aaatcaaaat ctttttctaa tgctgtgtcc aatccttcac gaccgtttgt
    54181 ttcaacaaca acatcatatc cttcatgtag tagttctaac gagacgaagc gagctaaatt
    54241 tttctcatct tcgattatta agatcttttt acccatctac accaaccttc ttattcttta
    54301 cttaacacta tcaattattt tttagaatat ctagataaaa aacaagatag cccacatttt
    54361 actaacgttt ctttaaataa ataaatcgcc atcccaaaca attgttcgat agcgagactt
    54421 aatttttatt attgagtagt gtcctctgtc acttttcaat tctactaaaa aaaaattaaa
    54481 atatcaactg atgttattaa taatttttat ctaatcacta tcaaacatac cttctaaggc
    54541 tgcgaaagga ttagattttt ctttttcttt agcttgtttt tcttcatact gcttttcagt
    54601 cattaacgac caatttttac ctgataaatc cgcttctacc ccaacctcat cagctgccag
    54661 aacacgaaga ggtatattta acaagatatt atcgataacg ctttcttcta aattaatatc
    54721 atcttcctct aatattaaga ccaaatcctg atcaacgagc tctttttttg tgctaacatt
    54781 ttcagcttca ataaaaactt cattaatagt ttgcttttca gataaaacaa ctggtttcat
    54841 agaacgacta gacggtaatg taatatcgta agccatatta tagtcaagta gatagagacc
    54901 atcttcatac accacatgac ctgaaacttg aatatcagaa atatccatta tctctgaatg
    54961 acgctccata agcgactctt tgatatctat ttttttatca aaataaagcc cctctggtga
    55021 ttttttgatt tctgtaagta acacgtgcca tctcccttta tagtatatgt catattatat
    55081 caagttttaa aggctttgtc ttaaaaaaac aaaacgcaag gcataactac cctacgtttt
    55141 gttaacagct acatatgacg caaacgctca atacgatctg caattggtgg atgcgtataa
    55201 aataaagact ttaacccttc ttttttcgtt gggtcattga tatagagagc cgcactagca
    55261 tcatctactg gatgccccat aggttcagaa cgatcaagtt tttctaaagc agagatcatc
    55321 ccttgtggat ttcttgttaa ctcaacagaa ctagcgtctg ctaagtattc gcgctgtctt
    55381 gaaatagcta attgaactaa actagcggct aatggtgcaa ggattagtga taaaatagag
    55441 aaaatcaaaa ccaagatgtt tcctccatct tctctgtcat catcacgcct acgacctccg
    55501 ccgtaaaata acatacggct accaatgctt gatattagag ttacagcact cgctaaagct
    55561 actgcaatgg ttgaaatacg gatatcataa ttacgaatat gactaacctc atgtccaata
    55621 acaccctcta gctcttcgcg gttcataaca gctaataggc ccgttgttgc tgcaacagca
    55681 gcattttctg gcttagagcc tgtggcaaag gcatttaggg agtcatcttc aacaatgaaa
    55741 acgcgtggca taggtatctg agctaccatt gccatatctt caacaatatg aaaataattt
    55801 ggcgcctcat cttctgtaac ttcacgcgca ttgttcattg acataacaac attagttgat
    55861 tgaaaaatca tacttactgc atagataacc ccaataatta gagctagaac aagcccaaat
    55921 tggtagctgc ctaataccaa ataacctaca gctgctccaa tagctgctaa aagacagaag
    55981 aagacaatta acaagacaac tgtttttctc ttattacttg caatttgttg atacagcaaa
    56041 agcctctctc ccttctcaat taaaagtcaa aggaaacttt tggcacttct ttttcttctt
    56101 caggagtttc taagaattgg cttggtttga acccaaatag ttttccaaca atattacttg
    56161 ggaaagtttc taattttaca ttataattag atgttgttgt gttatataac tgacgtgaat
    56221 aagaaatttt attttccgta ttagttagtt cttcttgaag tttaacaaag ctattgttgg
    56281 cttttaaatc tgggtaattt tcagcaaccg ctaaaatact tgaaagctgt cttgttagct
    56341 cattacttgc tgtcattgct tcagcaggtg tgttagcttt agcaacttga gcacgcagtt
    56401 ctgcaatctt ctcaagtgtt ttcccttcgt acgcagcata gcctttaaca gtttcgatta
    56461 ggtttggaat taaatcgtta cgacgtttca attgaacgtc tatttgactc catgattctt
    56521 tagtatgcat gcgacttcta actaaactgt tataagcaac aatcaaccaa ataacaaaca
    56581 aagcaataat tgctattaaa atcatagttc ccatagtagt agctccttta cattttttcc
    56641 attatagcaa ttttctttag attaggaaag tcctgttatt cccattatta gtatttgtga
    56701 taaaatagag tcattatacg atatagaaat tgaggtttac tatgacaccc caagcatttt
    56761 atcaagttct gatagagcat ggtattacac ttactgacaa acagaaaaaa caatttgaaa
    56821 cttattttag acttcttgtt gaatggaatg agaaaattaa cctaacagct attacagata
    56881 aggaagaggt ttacttaaag cacttctatg attctattgc gccgatttta caaggttata
    56941 ttgacaatag tcctcttagc attttagata ttggtgctgg tgcaggtttt cctagtattc
    57001 ctatgaaaat cctttatcct gaaattgata ttactattat tgattcactg aataaacgca
    57061 ttaattttct aaacatatta gctaatgaac ttgaactttc aggagttcac ttcttccatg
    57121 ggcgcgctga ggactttggt caagataaag tatttcgagc taaatttgat atcgtaacag
    57181 ctcgtgcagt agctagaatg caggttctag cagaattgac aatacctttt cttaaagtta
    57241 atggtcgctt gattgcactg aaagctgcag ctgctgaaga ggaattaatt tctgcagaaa
    57301 aagctttgaa aacactattt tcacaagtaa ctgtaaataa aaattataag ttacctaatg
    57361 gtgatgaccg aaatatcaca atcgttagca agaaaaaaga aacgcctaat aaataccctc
    57421 gtaaagctgg tactccaaac aagaagcctt tgtaaagttg ggagctagta tgaaacattt
    57481 ctttgattac aagacaatgt ctgtcgcacg taaactcagc attagtttta tcgctgttat
    57541 cctattggga agtattttac tttcacttcc aatttttcaa tatgctaacg cccctaaaac
    57601 acattacatt gatcaccttt tcacgactgt ttcaatggta tgtgtaactg gtttatctgt
    57661 ttttcctatc agtaaggttt ataacggttg ggggcagatt gtagctattt tactcatgca
    57721 aacaggggga cttggtttag taacactaat gtcgttgtct tactataccc taagacgtaa
    57781 aatgtcgtta aatgatcaga cccttttgca gtcagcgata acttataaca gttcgacaga
    57841 tttaaaaaaa tatctctata tgatttttaa agtaacttta actttagaag ttttagcagc
    57901 cagcatttta gccatagatt ttattcctcg atttggtttg ggtcatggta tctttaatag
    57961 tatattctta gctgtctcag ccttttgtaa tgccggtttt gataatctag gggcaacgag
    58021 cttagctcaa tttaaactaa atcctttggt taacattatc gtttgtttct tgattatttc
    58081 aggaggactt ggatttgccg tatggaaaga tttaattgaa gctactatac aaacatctca
    58141 caagggacca aaattaatca agacatttcc aaaacgcctc agcaaccatt ctaaactggt
    58201 tttaaaaaca acaaccatta ttttactaac aggaactttg ctttcttggt tgttagagtt
    58261 tggaaatttt agaacaattg ctaatttatc tctacctaaa caactaatgg tcagcttttt
    58321 ccaaacagtt acgatgcgta ccgctggatt ttcaactatt gattataccc aaactgattt
    58381 cgcaaccaac cttgtttata ttatccaaat gttaattgga ggagctcctg gtggaacggc
    58441 aggaggattt aaagtcactg ttatcgccat tttactctta ctatttaagg ccgaactgag
    58501 tggccaaagc caagtgactt ttcattatcg aacaattcct tccagtatta ttaaacaaac
    58561 actatcgatt ttaactttct tttttatcat acttattagc ggctatctcc ttcttctgga
    58621 actcaatccc catatagatc ctttttctct tttctttgag gcatctagtg ccttagcaac
    58681 cgtgggtgtg acaatgaaca ctaccaatca attaagtctg ggcggtcgaa ttgtaattat
    58741 gttcctcatg tttattggtc gagtcggtcc aattaccgta ctgctatcaa ttcttcagaa
    58801 aaaagagaag gaaattcact atgctgaaac agaaattatt ctaggttaga aaaggtcttt
    58861 tatgaaaaca aaaattattg gtgtacttgg tctaggaata tttggacaaa cactcgcaca
    58921 agaactaagt aactttgaac aagatgttat tgctattgac agcaatcctg aaaatgtaca
    58981 agctgtcgct gaagttgtta caaaagcagc tatcggagac attactgatt tagctttcct
    59041 aaaacacatc gggatcagtg actgtgatac tgttattatt gctacaggaa acagtttaga
    59101 gagctcagta ttggccgtaa tgcactgtaa aaagttaggc gtccctcaag ttattgctaa
    59161 agctcgaaac cttgtatacg aagaagtact ttatgaaatt ggtgctgatt tggttatctc
    59221 tccggagcga gaatctgggc aaaatgttgc tgcaaacctc atgagaaata aaattacaga
    59281 tgtcttccag attgaatctg atatttctgt cattgaattt aaaattccca aatcatgggt
    59341 agggaaaact gtcgaacaat taaatatccg tcacaagttt gatcttaacc tcataggtat
    59401 tagaaaagca aaaaacaaac ctgtcgatac cgaagttcca atcaatagtc cacttgaaga
    59461 aggtattatt ctagtggcta tcgctaactc agatgccttc caacgctatg actatttagg
    59521 atactttaac taaagaaaat aaaaaactga ctattaaggt cagtttttta ttgccatggg
    59581 ttatagaaac gtccatgatt aaggtgaatt aaataaaggc ttacaaacag actagctaga
    59641 gcaagtatga ttaagacaat gtcacttttt tccaaagatt gcttagagta ccaagtacgt
    59701 cttttatttt taccaaatcg tcgaagttcc atagcagttg ctactgtatc aatacgttct
    59761 agagagctaa aaattaatgg tgtgacaatc tgtaaattcc ccttaatgcg tgctactaag
    59821 ttcgattttt ttgataattc tattcctctt gcctcttgtg ctcgacgtat tgtataaaat
    59881 tcttcttgaa catcaggtat atatctaagg gtcaaactaa cagcataagc tattttatat
    59941 gataaaccta attgatttaa acttgatgcg aactgactag gattagttgt catcaaaaac
    60001 aaaagagcta aaggcactgt acaaaagtat tttaaaatca gattaaagag gtaaaacaac
    60061 tcttggctag tcacatcata cgggccaatt ccttttaaca ataaagaaga actaccataa
    60121 atcttatcac cataatgggg agcaaaaaga tagaccatta taatatttaa aacagcgaat
    60181 acaacaacta aagtcataac aaaggacacc tgttgccatt tgatttttgc taatctaaat
    60241 aagactagcg atcccaatga aacaagaatt aaaaatcttg tatcataagt ggtcatgcaa
    60301 gcaacagaaa ctaaaataaa aaatagtaac ttactggcac ctgatagtct gtatagaaag
    60361 ccataaccct cttgataacc aattaattga ttagccattc ttacctcctt gattatcaat
    60421 gtaccattgt gttagtttct gtggagatat ccctaatctg tctgctaaag caaataaact
    60481 cgttttcttt aaatgagtct ttttcaaaag ctctgtattt gaaaggatgt taataggatg
    60541 atcatctgca gcaataagac cattgtcgat tacaattgca cgatctgtat actctaacat
    60601 aagctgcata tcatgtgtaa tcataacaat gctatgtcca tcacaactta gtttgtcaag
    60661 gaaagacatc atttcagtat aatgcttcat atcttgacca gctgttggtt catccaaaat
    60721 aataacttct ggatttaaaa tcagtatcga agcaatcgta acacgttttt tctgaccgaa
    60781 tgatagagcc gatataggcc aattcctaaa ttggtagagc ccacaaacct ttaatatatc
    60841 gtagacacga ctttctatat cattatcact aaaacctctt aaacggagac ccagagcaac
    60901 ttcatcaaaa accatcgctt gacttatcat ctgattggga ttttgcaaaa catagccaat
    60961 tcgttctgct ctttctttta tagagtcttg actgacatct tcattattat aaattagagt
    61021 agcattatta gggataaatt gacataacgc tttagctaaa gtagatttac ctgcaccatt
    61081 tttcccaacg atagctaacc gctgaccttt atataaatca aacgaaacat catctaaagt
    61141 attattctct aaatcatatg acaccgacaa atgctgtact gataataatt tatctgtatg
    61201 tttatctaaa tctaatgctc gcattttagg tatcaatagt tcactgaaat caaagtcatc
    61261 aatagacgtc attgtatttt gtttttcaaa gtcatatcct aaatttttga gggcagaaat
    61321 atacagtggt tctcgaatac catattcaga taaaaaatta gtttttaaaa gttgatccgg
    61381 ctccccatta taaataattt gtccatcact aaaaagaact acacgatcta ctcgctcata
    61441 aagaacatct tccaatcgat gttcaataat aatagtggtc gcatcttgct cttgatgaat
    61501 acggtcaacc aatgccatga tatcttgtcc cgattgtgga tctagattag ccaaaggctc
    61561 atcaaataat agaattgggc tatcgtctac taaaactcca gctaaactaa cccgttgttt
    61621 ttgtcctccc gacaagtctt gtggacgttt agacaaaaga ggagctattt ctaaacgttt
    61681 tgcccacatt tcaacgatag aggccatttc ttcctgtgct acaacgtcat tttctaatgc
    61741 aaaagcaata tcttctacta ctgtcaatcc tataaattgg ccatctgggt cttgtaaaac
    61801 cgtagaaact aaatgagact tgtcatatat tgacaagtca aaagcatttt tatggttaat
    61861 cgtaaaaata ccactatatt ggcctttatg agtatttggg ataataccat ttaaacaatg
    61921 accaagcgtt gacttccccg accctgaagg tcctaatatc aagacctttt cacctttagg
    61981 aatgcttaag ttaatccctt ttaaagttgg ttcagattga acgtcatatt gaaaggtaaa
    62041 atccttccat tcaataaaat ctttcataga ttactctctt atcctaatct ttggataaac
    62101 tacctttttg tggtcgtgat ttagcgtatg ctagcaacaa aagcgtacca cctactccaa
    62161 tagtaatgga attcacaaga cttgataaga aaccttgtgc aaaaactttg cttgcaggtt
    62221 cgctataaaa gaaaatatca ccatacggtg caacgacaga ccaacctata aggttagcaa
    62281 taacttgaac tacattaaat agaagcaaat ccttcttagt catgccagat aattgaatag
    62341 ctaatttttt agcaaaaaag cctatcatca aacctaaaag accactaact aaaacccaag
    62401 tccaccacgg agaaccatat tgaatcgaat ctttcaatgc gtgtccgata aaaccagtaa
    62461 aaaaaccaac tcctggtcca taaataactg caaataatgc taaaaccgca tactgcaatt
    62521 gaatattagt atttggaatt ggtgttggta tattaactag catacctata atgataaaaa
    62581 gtgcagctcc aattccagtc gctacaacct tttttattgt gtttgtattc ataaaaacct
    62641 ctctgaaaaa caagcttaaa cagtcactta caatctgttt tattttatca tatttcaagc
    62701 tgatttgaaa ccttattgac aagaagaact gaaaaacgat tgaaaataat attaaatacc
    62761 aaaaaagatc tcagatagaa tacctaagac cttcattagt ttattttttt ccaaaaactg
    62821 atttttcagc tacagccgtg aacagaacat ctgtcgatga atttaaggct gtctcacacg
    62881 aatcctgtat cacccctaca atgaaaccaa ctcctacgac ttgcattgca acgtcatttg
    62941 aaataccaaa taagctacat gccaccggaa tcaataaaag tgaaccacca gttacaccag
    63001 aagctccaca tgctgataca gcagcaacca cacttaataa gaatgctgtt ggaaaatcaa
    63061 cagtaattcc taaagtattt actgctgcta aagtcaggat attaattgta atagctgcac
    63121 cagccatatt gatagctgca ccaagcggga tagatactga gtaagtgtct ttatctaatc
    63181 ctaaatcctc acatagtctc atattaacag gaatattcgc cgcagaacta cgtgtaaaga
    63241 aagctgtaat acctgaatcc tttaagcatc gtaataccaa agggtaagga tttttacgca
    63301 tcataacaaa agcaatgatt gggttaacga caagagctac aaataacatc gtacccacca
    63361 aaactaatat taaaagacca taatcaccta gtatagcaat accgttttct gaaatgctag
    63421 tgaaaaccaa ccccataata ccaattgggg ccaaattgat aatccatttg acaacttgtg
    63481 aagtagcatc agcaaaagtt tgcattaaat cttttgtacg ctgattactg cttcgcatcg
    63541 ccaaaccaaa aataaccgcc caaaatagaa tactcatata attggcctcg gtaatggcat
    63601 gaatgggatt atcaacaatt ttcaacaaaa gagattgaaa gacttctgaa atccccttgg
    63661 gaggtaataa gtctgtcttt gctggcgttt tcaatatcag tgtcaaaggg aaaatatagt
    63721 tcacaacaac agcgattagg gccgctgcaa aagttgcagt gatatacaaa ccaattatcg
    63781 ttgacatatt ggtcttaccg ccctcacggt gttgcgacaa ggcactaata actaaagtga
    63841 aaactaaaag aggggcaata gctttcaacc ctcctacaaa aagttgacca agtaaaccaa
    63901 tcgcagaggc tttagggact aaaataccca agaataagcc aataacgact ccaattgtta
    63961 ttttttttat caagcttgtt tttctccaag cttttaaaaa tcgtttcata ctaaactcct
    64021 aaaaaattct taaactctat gatatgataa aaaaaaagat tcgtcaaata ccttttttat
    64081 tttttagagg ttgacgaatt ctaaattttt ctgatataat tactttttga aatattcaat
    64141 caatttcgaa aataaaatat ttttttgtac agaaaggtac attgatgaaa ctaaaaaaca
    64201 cttatatcat tatcggcttg atgctttttg cgctgttctt tggagccggt aatcttattt
    64261 atccagccta cttaggtatc tactctggtt ctaattgggc gtgggctatc attgggttct
    64321 gcttaacagg cgtaacttta cccctacttg gtgttgcagc tgtggcgtac tcaggagcaa
    64381 ccgatgttga aagcttcacc aaacctgtct ctaaaacata tgctattttc tttgcagtag
    64441 cactatacct atctattgga cctttctttg ccattcctcg aacaggggca acctcattct
    64501 cagttggtat tcaacctatc ttaggcgaca gctccttagg caaaattatc tatggtttaa
    64561 ttttctttgg tatttcttat ttcttagcta ttagacctag tcagattgta gatcgtattg
    64621 gtaaatatct aactcccttt ttactaactg ttattagtat tttaattatt gcctcactca
    64681 tccatccagc tgctatcgtt ggaacaccac ataacgcttc aacgaatgtc agcgatgctt
    64741 tcaaggattt accattccta gcaggactga ttcaggggta tggtacaatg gatgcccttg
    64801 catcaattgc ttttgctatt atcgttattc aagcaagtaa acagtacgga gcaattacca
    64861 aaaaagaaat tacttctatg gcacttaaat ctggtgctat cgctacattt cttttagctt
    64921 ttatttatat ttttgtagga cgtattggtg caacttcaca aagcttgttt aaatttgcaa
    64981 atggttcctt cttacttcac aatactccaa tagacggtgg gcatgttctt agtcaatcag
    65041 ctaacttcta tttaggaatt gtgggacaag ctattttagg tacggctatt tttcttgctt
    65101 gcttaacaac agctactgga ttaatcactg cttgtgcgga atacttccac aaattacttc
    65161 caaagattag tcacattact tgggctacaa tctttacttt aattgctatt acgttctatt
    65221 ttggtggttt atcagagatt atccgttggt cactaccagt tctctacctt ctctatccat
    65281 taactatagt cttaattttc ttagtctttt ttgatcaaaa atttgaaagt agctgcattg
    65341 tctaccaaac ttctattgcc gccactgctg tcgctgcttt atacgatgcc ttatcaaaac
    65401 tcggcgaaat gactggttta tttaccattc catccgcttt gaccacattc tttactaaag
    65461 ttgttccttt aggtgaatat tctatgggat ggattagttt tgcaatttgt ggtgtcttag
    65521 ttggtcttat tctaaaaaaa gtaaaagcat aataatttaa agcagtacga aaattactgc
    65581 ttttatttta aaataattat tgtaaccgat ttcttttggt ggtataatag ccttgaaaat
    65641 aaataatttt tgaaggaact ctaatatgag agctgcaact tatctttcag ctaatgaatt
    65701 atcacttact gataaggcta aacctcaagt aataaaaccc acagatgcgg ttgtaagact
    65761 tgtcaaaaca accatttgtg gtactgatct acatatcctt ggtggcgatg ttcctgcttg
    65821 taaaccagga acaactttag gtcatgaagg cattggtatc gtcgaagaag ttggttctgc
    65881 tgtcaatcat tttaaagtcg gagataaagt aattatttct tgtgtcactg cctgtaatac
    65941 ttgctattac tgtaagcacc agttgccttc acattgtgaa gatggcgggt ggattttagg
    66001 acatcttatt gaaggtactc aagcagaata tgttcatatt ccacatgcag atggtagcct
    66061 ttatcatgca cctgaaggtg tttgtgacga tgcattggtt atgttatctg atattttacc
    66121 aacttcatac gaaatcggcg tcttaccttc tcacattaaa ccaggtgata ctgtttgtat
    66181 tgtaggcgct ggaccaattg gactatctgc cttactaact gcacaattct attctcctgc
    66241 caaaattatt atggttgatt tatctcaaaa acgacttgaa gcctctaaaa aatttggtgc
    66301 aacacacacc attctatcaa cgtctactca agaagttaaa gaagaaattg ataaaattac
    66361 caaaggccgt ggcgttgatg tcgtattaga atgtgttggt tatcctgcaa catttgatat
    66421 ttgtcaaaat gtagtttcta ttggaggaca tatcgcaaat gttggtgttc atggtaaacc
    66481 tgttgaattt aacctccaag atttatggat aaaaaatata acccttaata ctggattagt
    66541 taatgctaat acaactgaaa tgctccttga agttcttgag acaggcaaaa tcgatgcaac
    66601 acaactagta acacatcact tcaagctatc tgaaatcgaa gaagcttata aggtatttaa
    66661 agctgctgaa gaaaacaata ctctcaaagt tatcatcgaa aacgatatta ctccagcctg
    66721 ataattttta aacaaatgaa aatgtgagaa gaatagcctt ctcacatttt tatttatgac
    66781 tcaagcgacg tgttaaaaaa tcacctaaaa attggataaa gaaaattaat aataaaatca
    66841 gaatagtcgc tactaaagta atatcatcac gagaatagtt atatccttta gtaatagcaa
    66901 cagaacccaa tcctcctgca ccaatagcgc cagccatagc tgtttcacct actaaagaaa
    66961 tcaaagtaac cgttgatact cgaattaaat ctggtagacc ttcacgaaga taaactacaa
    67021 taatatccca aagtgttcca cctgaggctt gtgcagcctc aataactcca ccatcaagtt
    67081 cagctaaaac aacttgaact tgacgagcaa aaaatgggaa aactgccaaa gaaagaggta
    67141 ccaaagctgc tggtgaacca agtgttgttc ctacaattac gcgagttact ggcgcaatca
    67201 aagcaagaag aataatgaag ggcagagctc taaaaacaga aacaacttta tctaaaactc
    67261 caaatactaa tttattagca ataactcctc taggactagt taaaacaagg aataaacctc
    67321 ctaacaatcc cattaaacct ccaataagga acgaccaaaa agtcatataa agggtttgta
    67381 caatagctgt ctgccagccg taagcacctt cccaacccat ttgatataca tttggtaaat
    67441 gtgtttgaat ccactctatc atgctttacc accttctttc aataacgtta attgaacttg
    67501 cgaatctgta attgcttggc aggcttgtct taatttttct tcgtcacctg ataagataac
    67561 taccatttct ccaactggaa taccatctaa aatttcaata ttcccataga ggatgttagc
    67621 agttacttca aattctttat aaatttgatt taaaagcggc tcatccgtgc tatgcccagc
    67681 gtatttcagt tgaactaatt tgctacctac tggtagattt ttgacaactt cttgttgctc
    67741 aatcttaagc attgcctcat caatcccagt agctattttt ataaaatctt gtgtaagact
    67801 ctctctagga tgtgaaaaaa tgtctaaaac tgatccttct tcaatcaatt taccattttg
    67861 cataacagca acgcgattag caatgtcttt gacaatttgc atctcatgtg taatcaaaac
    67921 aatcgtaagt cccaattttt tattcagatc ttggagaagg gcaagtattt gttttgttgt
    67981 tttagggtca agtgcagagg tagactcatc tgaaattaag attttaggat cgttagccaa
    68041 tgcacgcgca atggcaacac gctgtttttg ccctcctgat aattgtgaag gatagttttg
    68101 agcacgatcg gataatccaa caagttctaa taatttagca accttagctg ctttcgcctc
    68161 ttttgataaa cctgaatgtt taagagcaaa agctacattc tgctctgctg tcaactgtga
    68221 cattaggtta aaatgttgaa aaatcattcc aatttcacgg cgctgttcgc gtaattgtgt
    68281 gctagtcagt gtcaccttgt tgtcgtaaat aacttggtca tcaatagtaa ttttacctgc
    68341 tgaaggttct tgtagcaaat taataacacg aacaagggta gattttccgg ctcctgaata
    68401 accgacaatt ccatatatat caccttgatt aatatggata gtaacatctt taacagcatt
    68461 aatctcacgt ttcttttggt ggaaagtaac atcaatgttg tctaatttaa taattggttc
    68521 tttactcata tgattctatt aaagcctcca ctaattcaat atgggtgtaa taatcagcaa
    68581 tactaacatt ttcatcaacg ccatggtctc gactattggc atgtcctatg ccaaaagctg
    68641 caattggcgc tcttagagct tgatgaacta aatacattgg tcctgtacct ggtgaggtgg
    68701 gtaacagaga taccccttct ggataaaatt gctcagctaa atcaacaact tttaggatag
    68761 atggcgctga catatcacta cgataagaca tttcaccaag ggtataggtt agctcaatgt
    68821 cattaaagcc attctctttc aaatgatttt gtattgaatc tagtacacct ttcggggtta
    68881 accctggcac taacctaacc tctgctttac atttagcata agcaggtaaa attgttttga
    68941 cgccttcacc ttgataacct gaggttatac cttcaatggc tatagaaggt tcaaaataaa
    69001 gctttctcaa aaaagtttta tgactatctg ctaaactggg caaaactaat tggtaagcat
    69061 cttctagcgc ctttgcagat ctataagaat atttttcaac taattctaac tctctcttag
    69121 tagggggaat aactttatca taaatacctt ctaccaatat atgacctttg ttatccctta
    69181 acgtattgag ggcttgaatt aaataccatg tcgaggaatc aacaacccca ccaaaggatg
    69241 aatgaatatc aacatccgct gaacttactg ataaatcaaa agtgacaatg cctttatttc
    69301 caccagcaat ttccaactgt cctttaggat ttttagggcc atcttcccac acgatcaaat
    69361 cagctccttg tagctgctct tgatactttt ctaaataata atcaaggcct actgaagctg
    69421 attcctccgc tccttccaca ataaaagtga tatctagagg aagttcccct ttgtgtcttg
    69481 ataaatattt tttaactgct gataaacgtg cggtaatgtg acctttatca tcatctacac
    69541 cacgtccata catcttacca tatcgaagtg aaagtgtaaa agggtcctca gtccactgtt
    69601 ctacctcatc tgctggtaca gtatcataat gattgtaaaa aataatacgc ttagcatcta
    69661 cttttgatga tttgaaattt gccacaataa agggagcgct atagctgtca tctataatga
    69721 cttctgcgcc agcttttcta aaaacttctc caagatagct tgctaagtca tttaaaccaa
    69781 ctttctgagc aaaaattgac ttaattgcaa ttaaatctct taaaatattg atatcatcct
    69841 gaacaatagt atcagataga aacttttgaa tttgattttc ttcctttgtt aaagtcatct
    69901 ttcatccacc ttccttcaaa aacaatccaa gagcctggca cattgtattc gtcccaggcc
    69961 ctttatggct atatggctga taagttgtat cctattagga ttccctgtta aacttataca
    70021 gtcaattcac gttaccattg tggaatatca gctgaagtat ctttgataac ttttttcact
    70081 tcatctgtgt gataagcatc caagatagct tggatagctt tagcgttctt ttgctttttc
    70141 caatttttac gtcccgcaat gatattaatc cattgttttg aatttttatc tgatttctca
    70201 acaaagatag catctgaagg ttttaaatta gcttgctcaa tgtatgtatt attaataata
    70261 gctgcatcta catctttgag tgcacgtggt gtttgactcg catctaactc ctgaatatta
    70321 atatcttttt tattagatgt gatattagca actgttgcaa ccttcttacc agaaacattc
    70381 aatttgatta aacctgctga ctgaaggaca tacaatgcac ggctaccatt tgttgcatca
    70441 tttggaattg caatagtggc tccttttttc aattttttaa gagattttac cttctcagaa
    70501 tagatacgaa ttggggctaa gtaagtcttt tcaagtggaa ttaagttttt cttattttcc
    70561 ttattccagt tttctaagaa attgtaatgt tgaaaggcat taatatccac atccttattg
    70621 gctgtcgctt gatttggttg tgtataatct gtaaattctg tgaatttgat tttagcttta
    70681 tcgcctacta gcttttcaat tttatcccaa cgtgcttttt cagtgtcaga aaaggtcata
    70741 acaccaactt taacaacttt acttgaagtt gagcttgctg aaacttcttg attactaaat
    70801 gtgactccta aaagtgttgt taaggctaat cctaagacaa tatgttttaa cttcataata
    70861 aattccctca atttttgtga taaagatatt atcccatagg aagttattag aatcaaattg
    70921 ttataaactg tctcttgtta tagttttaaa ctatataaca aaaactaagg attgacatct
    70981 ccttagtttt ttattactac ttctccttag gagtaacgag atctttctca tttggtacgt
    71041 aatcacctcc aaggtattct ttagctattt ttgaaagtgt cccatcttta cgtaattgtt
    71101 taaggcaatt attcacatac ttttgcaagt ctttttgatc ctgaccaaag atgaaataaa
    71161 tataaggctg atctctcata gtcaaaggga tggtttttaa attcgtgaga ccttgattct
    71221 ttatgatagc gttaaccgtt agtccgtcaa aaagtttaaa atcagctttt ccatcactca
    71281 aattcgttag aatctgtgta atattttcat tagtatattt tagagtaaca ggatttttct
    71341 gatgcttttt attgaaattt tctaattgct tggcagttgt cgttccttgg acaacctgtg
    71401 ttttatgacc agtaatatca ttataagctt tgatattact attcttagga actgctaaaa
    71461 ctgaaggagt agagcctatt gggtatgaaa ataaatattt ttgagatctc tctgatgaat
    71521 aagaaatatt atttccaccc atttgaaact ttcctgaatc aataccggta aataccgatg
    71581 accactctgt ttttttaaaa gtgactttat agttatctga acctttaaaa acagctttgg
    71641 ctacttcaat atcaaagcca gtcagtttcc catcctttac ataagaaaaa ggtgccgttg
    71701 ttccgacagt tgcaaaatta acttcttttt tgcccgtctt ggtagtatga gattttgagc
    71761 tacaagcagc tagagacgct actgtcacta aaataattcc tgttacactt aacacttttt
    71821 ttattgtcat cttttttctc ctctttaatt gtacacgata tcataatatc tttaagtttt
    71881 taactaacac ttatcttcta tcatctgaga gtttttgcta ttatatggat gctacgtttc
    71941 attaacaaaa taaccaaata aggtttggtt ttcaatttgg tgaagcatta attctggatg
    72001 ccattgaaca cctataatac attgttctgt tcgactttca taagcttcta ctgtcccatc
    72061 tctaggatct ctagcactaa ctatcagacc ttcagctaac agtttaacac tttgtctatg
    72121 aagagaattg atcctaaaat cacctgatat aaagccttct agaaaactcc ctttttccac
    72181 atttaaataa tgagcaaatc caatgtaagg ctcttgatag tgattatcaa tagattgatt
    72241 aagtgtacca ccaagtgcca catttactaa ctgcatgcca cggcaaatcg caaagattgg
    72301 tttattttgt ttcaacgctt cgtcgactaa tgcaaattca aaaatatcac gcgcaagtga
    72361 ataatcatca ctctcaatta tcttctcttc accataatag gatggtaaaa cattttggcc
    72421 accagatatg atcagcttat ctatcatctc aacataagct ttggcacttt ctgcttcact
    72481 tattggtagg ataactggta acccgcctgc gtttttaact ccttccgaaa tatgcctaga
    72541 taccgaatca taataataac ctggaatatc tgacatttct ctttcatttc ctgtaatacc
    72601 aataataggc tttgttaaca ttttgctccc tcatcaacca ataactgtct aataattatc
    72661 attttacacc taaaaaaact ttttgaataa tattttttac ctatcacttc tttaacaata
    72721 cctatcaact ctcacaaaaa taaaaaccat agtattaata ctacggtttt attttttaga
    72781 aatctgctac attatgataa actttttgaa catcatcatc tgattcgagt gcgtcaataa
    72841 gcttttcaaa agtttcaagg tcatcacctt ccaatactac ttctgattga ggaatcattt
    72901 caagttcagt aacttggaat tcttctacac cattatcgcg aagtgcttgg atacctttat
    72961 gaagatctgt tggggcggta taaactgtta ttgttccctc ttctgcttca acatcatcta
    73021 cgtctacatc cgcttcaagt aattgttcga agacagtgtc agcatcatca ccagcaaaaa
    73081 cgatgacacc ttttttatca aataagtagg ataccgatcc tgaagctccc atattgccac
    73141 cgttcttacc gtaagcagta cgtacatttg ctgccgtacg gttaacattt gatgtcaaag
    73201 tatccacaat aatcattgaa ccatttggac caaaaccttc atagcgtccc tctacgaaag
    73261 tttcatctgt gtttcctttg gctttatcaa tcgctttatc aataacatgc tttggaactt
    73321 gtgcttgctt agcacggtcc aaaacgaatt ttagagctga gtttgactcg gggtctggtt
    73381 caccttgctt tgcagcaaca tatatttcaa caccgaattt agcgtatact tttgagttag
    73441 caccatcttt agcagtcttt ttagcaacaa tattggccca cttacgtccc attggaaaat
    73501 ctcctttgat ttcattttta ataacctgct ggctcatatt ggcaagcaat cttaatttta
    73561 actctattat tatagcactt tgatagcaat tggtaaaatg gaaattctat caccaagctc
    73621 tagcatctgt cttatgtttt aagtaatcaa tccatctact atcctttaca cgacacaagg
    73681 taactgataa accagccata tctaaacttg taattaggtg gcctgtttta ataaaaggta
    73741 attgtaaatc gtctaaagcc agaaattcca tgacagcatt tgtaaaaagt aattcctcca
    73801 ttttagaact tcctccaaga ttatttatca atagaatgaa tgcttctcca tcttgccaac
    73861 gtaatttcat tttcaatttg ttaactagtt cgtttgccaa atgttccatg gacacgaaag
    73921 gcattgtccg gtatcctggc tcaccatgaa taccgattcc aaatgagatg taaccttctt
    73981 ctaaatcaaa aacaggttga tgctgaccta aaatagtggc tgacttactt gctacaccta
    74041 aagtagccat tgaagttgtc aaagatagtc ctagctgttc taattcatct aagcttgctc
    74101 cttctaaagc agcttgtcca ataattttat gtagcaacac agtaccagca acgcctcgat
    74161 gacgaatttt aaaatttgat gtttcaacag agatgtcatc atgagaaacg atgtatttga
    74221 ttggaatgcc ttcttgacga gcctgttcaa tcgcttggga aaattcttcc aaatcagctt
    74281 cgaaattttt gataataaca aaaacacctt tgcctcgatt gataaatcta atagtctcta
    74341 gaatatcact agcacatggt ggtacaaaaa taggaccact aatcgctgcc gatagcattc
    74401 cctcaccaac atatccaaag tgagctggct catgtccgct gcctcctcct gataaaatcg
    74461 gaattacttg ttcggattgc cctaaataat gaatagcggg caaatctttt atttgctcta
    74521 ctttagggtt agttttagtg attgctttta ttgtttcaaa aacatattcc attttatgat
    74581 gttctgtttt tctcatatct ttgtcctaat aattttctat tgatgttctg ataatttttt
    74641 caatgacttt cctattaaat aattcttctt ttgagttatt tattaactca tattttataa
    74701 aataag
//
LOCUS       DATRTN010000002        64702 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.002, whole genome shotgun sequence.
ACCESSION   DATRTN010000002 DATRTN010000000
VERSION     DATRTN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 64702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64702
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.002"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..418)
                     /locus_tag="VBE22_000082"
     CDS             complement(<1..418)
                     /locus_tag="VBE22_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEO7201564.1"
                     /translation="MKQEKTGRNLNFWKWAFLLLLAINLSFTAVIASRLIQVREPNTG
                     KISTGVQDKVKVGTFTTNKSQLNKTIALYLKQYQTKKMNYKIYAASSSILFEGSYQLL
                     GYEVPLYIYFEPYRLTNGAVQLKVTSFSVGTLPLPEK"
     gene            complement(393..1232)
                     /locus_tag="VBE22_000083"
     CDS             complement(393..1232)
                     /locus_tag="VBE22_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO7201565.1"
                     /translation="MNKKKILTGLSFFLVSLLLSFGIFSLIIPKSNPKLTKKDFLTKK
                     VIPLNYVALGDSLTEGVGDTTSQGGFVPLLSESLHNRYSYQVTSVNYGVSGNTSQQIL
                     KRMTTDPQIEKDLEKADLLTLTVGGNDVLAVIRKELSHLSLNSFEKPAEAYKERLKEI
                     LAKARQDNPKLPIYVLGIYNPFYLNFPQLTKMQTVIDNWNKATKEVVDASENVYFVPI
                     NDRLYKGINGKEGITESSNSQASITNDALFTGDHFHPNNIGYQIMSNAVMEKINETRK
                     NWP"
     gene            complement(1225..2061)
                     /locus_tag="VBE22_000084"
     CDS             complement(1225..2061)
                     /locus_tag="VBE22_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO7201566.1"
                     /translation="MSKIKIVTDSSITIEPELIKELDITVVPLSVMIDGTLYSDNDLK
                     AQGEFLNLMRGSKELPKTSQPPVGVFAEIYEKLMNEGAEHIIAIHLTHTLSGTIEASR
                     QGANIAGADVTVIDSTFTDQCQKFQVVEAAKLAKEGADLDTILARVEEVRQKSELFIG
                     VSTLENLVKGGRIGRVTGLLSSLLNIKVIMELTNHELVPIVKGRGLKTFSKWLDNFVE
                     SAQTRKIAEIGISYCGKADMANNFKEKLAVLGAPISVLETGSIIQTHTGEDAFAVMVR
                     YE"
     gene            complement(2174..3832)
                     /gene="recN"
                     /locus_tag="VBE22_000085"
     CDS             complement(2174..3832)
                     /gene="recN"
                     /locus_tag="VBE22_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEO7201567.1"
                     /translation="MLLEISIKNFAIIEEISLNFETGMTVLTGETGAGKSIIIDAMNM
                     MLGARASVEVIRHGANKAEIEGFFSVEKNQSLVQLLEENGIELADELIIRREIFQNGR
                     SVSRINGQMVNLSTLKAVGHYLVDIHGQHDQEELMKPNMHILMLDEFGNTEFNVIKER
                     YQSLFDAYRQLRKRVLDKQKNEQENKSRIEMLEFQIAEIESVALKSDEDQTLLKQRDK
                     LMNHKNIADTLTNAYLMLDNEEFSSLSNVRSAMNDLMALEEFDREYKDLSTNLSEAYY
                     VIEEVTKRLGDVIDDLDFDAGLLQEIENRLDVINTITRKYGGDVNDVLDYFDNITKEY
                     SLLTGSEESSDALEKELKILEHDLIESANQLSLERHKLAKQLENEIKQELTELYMEKA
                     DFQVQFTKGKFNKEGNEIVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKSAFSRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIHKIGSHGQVLAISHLAQVIAIADYQYFIEKIS
                     SDSSTVSTVRLLSYEERVEEIAKMLAGNNVTDTARTQAKELLGS"
     gene            complement(3844..4317)
                     /locus_tag="VBE22_000086"
     CDS             complement(3844..4317)
                     /locus_tag="VBE22_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEO7201568.1"
                     /translation="MKKSERLNLIKQIVLNHAVETQHELLRRLEAYGVTLTQATISRD
                     MNEIGIIKVPSAKGRYIYGLSNENDPIFTTAVAKPIKTSILSISDKLLGLEQFININV
                     IPGNSQLIKTFIMSHCQEHIFSLTADDNSLLLIAKSEADADHIRQSMIAMLEKKD"
     gene            complement(4304..5131)
                     /locus_tag="VBE22_000087"
     CDS             complement(4304..5131)
                     /locus_tag="VBE22_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEO7201569.1"
                     /translation="MAKERVDVLAYKQGLFDTREQAKRGVMAGLVINVINGERYDKPG
                     EKVADDTELKLKGEKLKYVSRGGLKLEKALQVFEISVADKLTIDIGASTGGFTDVMLQ
                     SGARLVYAVDVGTNQLVWKLRQDHRVRSMEQYNFRYAQKEDFKEGLPEFASIDVSFIS
                     LNLILPALKEILVDGGQVVALIKPQFEAGREQIGKNGIVKDKLVHEKVLTTVTNFTKD
                     YGYTVKHLDFSPVQGGHGNIEFLMHLQKCQDPQNLVLDQIQDVIEKAHKEFKKNEEE"
     gene            complement(5124..5996)
                     /locus_tag="VBE22_000088"
     CDS             complement(5124..5996)
                     /locus_tag="VBE22_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO7201570.1"
                     /translation="MVTIEKIDEAIHRYYKQTHSVVSPDLIKAILYSVDGGGKRIRPR
                     ILLEILEGFGVELIDGHYDVAAALEMIHTGSLIHDDLPAMDNDDFRRGRLTNHKKFDE
                     ATAVLAGDSLFLDPFDLVVKAGFKADVTVRLIELLSMSAGSFGMVGGQMLDMKGENKV
                     LSIDDLSLIHINKTGRLLAYPFVAAGILAEKSEEVKGKLHQAGLLIGHAFQVRDDILD
                     VTASFEELGKTPNKDIVAEKTTYPNLLGLDKSQEILDDTLKKAQAIFQNLEKKANFNA
                     RKIIDIIEGLRLNG"
     gene            complement(5996..6211)
                     /locus_tag="VBE22_000089"
     CDS             complement(5996..6211)
                     /locus_tag="VBE22_000089"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEO7201571.1"
                     /translation="MSDKKTFEENLQELETIVSRLETGDVALEDAIAEFQKGMLISKE
                     LQKTLKEAEETLVKVMQADGTEVEMDT"
     gene            complement(6189..7529)
                     /locus_tag="VBE22_000090"
     CDS             complement(6189..7529)
                     /locus_tag="VBE22_000090"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEO7201572.1"
                     /translation="MSDYLSVSTLTKYLKLKFDKDPYLERVYLTGQVSNFRRRPNHQY
                     FSLKDDKSVIQATMWSGQFKKLGFELEEGMKVNVVGRVQLYEPSGSYSIIVEKAEPDG
                     IGALAIQFEQLKKKLSQAGYFDDRHKQLIPQFVRKIGVVTSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGEGAAQEIAQTIALANEKKDLDLLIVGRGGGSIEDLWAFNEECVV
                     EAIFESRLPVISSVGHETDTTLADFVADRRAATPTAAAELATPVTKIDILSWITEREN
                     RMYQSSLRLIMTKEERLQKSKQSVIFRQPERLYDGFLQKLDNLNQQLTFSMRDKLQTV
                     RQKQGLLHQKLQGIDLKQRIHTYQERVVQSRRLLSSTMTSQYDSKLARFEKAQDALIS
                     LDSSRIVARGYAIIEKNHTLVSTTNGINEGDHLQVKMQDGLLEVEVKDVRQENI"
     gene            complement(7655..8491)
                     /locus_tag="VBE22_000091"
     CDS             complement(7655..8491)
                     /locus_tag="VBE22_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEO7201573.1"
                     /translation="MIVGEQEARALIKPRPKSSHKGDYGSVLLIGGFYPYGGAIIMAA
                     LACVKTGAGLVTVATQSCNIPSLHSQLPEVMAFDSDDYKWLEKSIVQSDVIVIGPGLG
                     VSESSRKILNQTMEKIQSHQSVIIDGSALTLLSEGAFPQTKAKNLVLTPHQKEWERLS
                     GIAVSQQTKENTQTALKSFPKGTILVAKSSHTRIFQDLDEKEIIVGGPYQATGGMGDT
                     LCGMIAGMLAQFKEASPLDKVSVGVYLHSAIAQELSKEAYVVLPTTISDEIPKEMARL
                     SK"
     gene            complement(8488..9342)
                     /locus_tag="VBE22_000092"
     CDS             complement(8488..9342)
                     /locus_tag="VBE22_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEO7201574.1"
                     /translation="MTELIDGKALSQKMQAELGRKVERLKEQHGIIPGLAVILVGDNP
                     ASQVYVRNKERSALEAGFKSETLRLSESISQEELIDIIHQYNEDKSIHGILVQLPLPQ
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPMMVPCTPAGIMEMFREYHVDLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRNLSEVTKEADILIVAIGQGHFVTKD
                     FVKEGAVVIDVGMNRDENGKLIGDVAFEQVAEVASMITPVPGGVGPMTITMLLEQTYQ
                     AALRSVSL"
     gene            complement(9481..11175)
                     /locus_tag="VBE22_000093"
     CDS             complement(9481..11175)
                     /locus_tag="VBE22_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO7201575.1"
                     /translation="MNYKEIYQEWLENDSLGKDIKSDLEAIKGDESEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAIARGIAVSYDVRYQSKEFA
                     ELTCSIMAANGIKSYIYKGIRPTPMCSYAIRALGCVSGVMVTASHNPQAYNGYKAYWK
                     EGSQILDDIADQIANHMDAITDYQQIKQIPFEEALASGLASYIDESIEEAYKKEVLGL
                     TINDTNIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKAFAYSESLGKSVDADILLATDPDCDRVALEVKDSKGEYIFLNGNKIGALLSYY
                     IFSQRCALGNLPHHPVLVKSIVTGDLSKVIADKYNIETVETLTGFKNICGKANEYDIS
                     KDKTYLFGYEESIGFCYGTFVRDKDAVSASMMVVEMTAYYKERGQTLLDVLQTIYDEF
                     GYYNERQFSLELEGAEGQERISRIMEDFRQDPILQVGEMTLENSIDFKDGYKDFPKQN
                     CLKYYFNEGSWYALRPSGTEPKIKCYLYTIGCTEADSLSKLNAIESACRAKMNSTK"
     gene            complement(11462..12196)
                     /locus_tag="VBE22_000094"
     CDS             complement(11462..12196)
                     /locus_tag="VBE22_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7201576.1"
                     /translation="MVEPIISIQGLHKSFGKNEVLKGIDLDIHQGEVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGIDITDKKNDIFKMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGLSKLDAQTKAYELLEKVGLKEKANAYPASLSGGQQQRIAIARGLAMNPD
                     VLLFDEPTSALDPEMVGEVLTVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDAGII
                     VEQGTPKEVFEQTKEIRTRDFLSKVL"
     gene            complement(12189..12881)
                     /locus_tag="VBE22_000095"
     CDS             complement(12189..12881)
                     /locus_tag="VBE22_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7201577.1"
                     /translation="MNFSFLPQYWSYFNYGVMVTIMISTCVVFFGTIIGVLIALVKRT
                     NLHFLTILANFYVWVFRGTPMVVQIMIAFAWMHFNNLPTISFGVLDLDFTRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPSGQIEAAYSLGIRPKNTLRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPVAPLLFAAFYYLMLTTILSALLKQM
                     EKYLGKGVKIDG"
     gene            complement(13023..13253)
                     /locus_tag="VBE22_000096"
     CDS             complement(13023..13253)
                     /locus_tag="VBE22_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HEO7201578.1"
                     /translation="MESHLVRIINRLELMATDGGNLKRNFEREGVVVAEVSFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            13551..15812
                     /locus_tag="VBE22_000097"
     CDS             13551..15812
                     /locus_tag="VBE22_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7201579.1"
                     /translation="MLCQNCKLNESTIHLYTNVNGKQKQVDLCQNCYQIIKTDPNNPL
                     FSGLNHVSHAPGGINPFFDDFFGDLNNFRAFNGQDLPNTPPTQSGGNRGGGNGNGRNN
                     NRNQTATPPQAKGILEEFGINVTEIARHGDIDPVIGRDSEIIRVIEILNRRTKNNPVL
                     IGEPGVGKTAVVEGLAQKIVDGNVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKL
                     MEEIRQRQDVILFIDEIHEIVGAGTAGEGSMDAGNILKPALARGELQLVGATTLNEYR
                     IIEKDAALERRMQPVKVDEPSVEETITILKGIQKKYEDYHHVKYNNDAIEAAAVLSNR
                     YIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDQRLIEAENLKVQATREEDYERAA
                     YFRDQIAKYKEMQQQKVDDQDTPIITEKTIEHIIEEKTNIPVGDLKEKEQSQLINLAD
                     DLKQHVIGQDDAVIKIAKAIRRNRVGLGSPNRPIGSFLFVGPTGVGKTELSKQLAIEL
                     FGSADSMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEIE
                     KAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGSGKTEASVGFGASREGR
                     TNSVLGQLGNFFSPEFMNRFDGIIEFKALDKENLLNIVDIMLSDVNARLAINGIHLDV
                     TDKVKEKLVDLGYDPKMGARPLRRTIQEHIEDAITDYYLENPSEKELRAIMTSNGNII
                     IKSSKKTEESAKG"
     gene            15998..16453
                     /locus_tag="VBE22_000098"
     CDS             15998..16453
                     /locus_tag="VBE22_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7201580.1"
                     /translation="MTNPTFGEKIDNVDYRSRFGVYAIIPNPTHDKIILVQAPNGAWF
                     LPGGEIEENENHLEALTRELIEELGYSATIGHYYGQADEYFYSRHRDTYYYNPAYIYE
                     VTAYHKEQAPLEDFNHLAWFPIQEAKEKLKRGSHRWGVQAWEKNHHSRK"
     gene            16517..16819
                     /locus_tag="VBE22_000099"
     CDS             16517..16819
                     /locus_tag="VBE22_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEO7201581.1"
                     /translation="MRLINTTSSHPELVRNQLQNTDAKLVEVYSAGNTDVVFTKAPKH
                     YELLISNKYRAIKDEELEAIREFFLKRKIDQSIIIQEQMKSLHTAKLIEISYPTTA"
     gene            complement(16936..19728)
                     /locus_tag="VBE22_000100"
     CDS             complement(16936..19728)
                     /locus_tag="VBE22_000100"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEO7201582.1"
                     /translation="MKLKETLNLGQTAFPMRAGLPNKEPQWQEAWDQADIYKKRQALN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPYVPGWDTHGLPIE
                     QVLAKKGVKRKEMDLAEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTPDYE
                     ADQVRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTVTASRGLTVGPDMEYVVVAPVGSERKYLLAEVLVDSLAAK
                     FGWENFEIVTHHTGKELNHIVTEHPWDTEVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLDVVVTVDSRGLMMENAGPDFEGQFYDKVTPLVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKTNFQPEWGKKRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFAEYGSIVWWQRDAKDLLPAGY
                     THPGSPNGLFEKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGEKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILKQTSETYRKIRNTLRFLIANTSDFNPKQDAVAYENLGAVDRYMT
                     IKFNQVVDTINKAYAAYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSPERRR
                     MQTVFYDILVKLTKLLTPILPHTAEEIWSYLEHEEEEFVQLAEMPVAQTFSGQEEILE
                     EWSAFMTLRTQAQKALEEARNAKVIGKSLEAHLTIYASQEVKTLLTALNSDIALLMIV
                     SQLTIADEADKPADSVSFEGVAFTVEHAEGEVCERSRRIDPTTKMRSYGVAVCDASAA
                     IIEQYYPEAVAQGFEA"
     gene            complement(20013..20783)
                     /locus_tag="VBE22_000101"
     CDS             complement(20013..20783)
                     /locus_tag="VBE22_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEO7201583.1"
                     /translation="MPLTALEIKDKTFSSKFRGYSEEEVNEFLEIVVDDYEDLIRRNR
                     EQEQYIKDLEEKIAYFNEMKESLSQSVILAQETAERVKISAQDEASNLMGKATFDAQH
                     LIDEAKLKANQILRDATDDAKRVAIETEDLKRQSRVFHQRLLSELEGQLKLANSSAWE
                     ELLKPTAIYLQNSDASFKEVVEKVLDEDDALPVVDDTESFDATRQFSPDEMEELQRRV
                     EESNKQLEESGLLDTNNFQMEEPINLGETQTFKLNIED"
     gene            complement(20793..21581)
                     /locus_tag="VBE22_000102"
     CDS             complement(20793..21581)
                     /locus_tag="VBE22_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO7201584.1"
                     /translation="MTLDDIYQHFRPEEYAFINKIDHLAQYVENTYSFITTEFLNPRE
                     FKILESVLERRGSHYYTSGQYFQTEYVKVIIAPEYYQLDMVDFNLSLIEIKYNAKFNH
                     LTHAKIMGTLLNYLGVKRSILGDILVEEGCAQVLVDSQMTNHLVHSVTKIGTASVQLA
                     EVPLSKLLTPKQDIQKLTVIASSLRLDKILATILKISRTQSTKLIEADKVKVNYAIVN
                     RVSEQLVEGDLISVRGYGRFTLNHNLGLTKNQKYKLEVDKMIHN"
     gene            complement(21583..21837)
                     /locus_tag="VBE22_000103"
     CDS             complement(21583..21837)
                     /locus_tag="VBE22_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEO7201585.1"
                     /translation="MILVLLILLKTIRVYSYILLAYALLSWFPGAYDSWIGRLVVQIV
                     DPIIRPLRRFNLQFLGLDFTVIVAFFLLNMLSRFLILLFT"
     gene            complement(21840..22445)
                     /locus_tag="VBE22_000104"
     CDS             complement(21840..22445)
                     /locus_tag="VBE22_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEO7201586.1"
                     /translation="MALKDRFDKIISYFDTDDVSENEVHEVQERTSVQRDSRAATAQE
                     ASQRSHMTNSAEEEMIGSRPRTSTYNPNRQERQRVQRDNAYQQATPRIQNKDSVRQQR
                     EQVTIALKYPRKYEDAQEIVDLLIVNECVLIDFQYMLDAQARRCLDYIDGASRVLYGS
                     LQKVGSSMFLLTPANVMVDIEEMNIPKTGQETSFDFDMKRR"
     gene            complement(22457..23131)
                     /locus_tag="VBE22_000105"
     CDS             complement(22457..23131)
                     /locus_tag="VBE22_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEO7201587.1"
                     /translation="MMNLQENKTAIFDNVSKLALKAGRAPESVHVVAVTKYVNCQTTE
                     ALIRTGVNHIGENRVDKFLEKYQALKDEKLTWHLIGSLQRRKVKDVINYVDYFHALDS
                     VKLAAEIQKRAQKPVKCFLQVNISREDSKHGFTIEQIDDALNLISRYDKIELIGIMTM
                     APLKATKEEISSIFEETESLRKRLQARNIERMPFTELSMGMSRDYDIAIQNGSTFVRI
                     GTSFFK"
     gene            complement(23137..24417)
                     /gene="ftsZ"
                     /locus_tag="VBE22_000106"
     CDS             complement(23137..24417)
                     /gene="ftsZ"
                     /locus_tag="VBE22_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEO7201588.1"
                     /translation="MVFSFDTASVQGAVIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEVLTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVITRPFGFEGNKRSNFAIEGIQELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERITEAARKAIYSPLLETTIDGAEDVIVNVTGGMDMTLTEA
                     EEASEIVSQAAGKGVNIWLGTSIDMDMKDEIRVTVVATGVRKDKTNQVSGFTTSAPTN
                     QAPSERQSTSNSNFDRRGNFDMTESREMPTQQNQPHTQNQQQSSAFGNWDLRRDNISR
                     PTEGELDSKLSMSTFSENDDMDDELETPPFFKNR"
     gene            complement(24439..25812)
                     /gene="ftsA"
                     /locus_tag="VBE22_000107"
     CDS             complement(24439..25812)
                     /gene="ftsA"
                     /locus_tag="VBE22_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEO7201589.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFIANEMNVIGVSNVPSSGVKD
                     GIIIDIEAAATAIKEAVKQAEEKAGITIDKINVGLPANLLQIEPTQGMIPVPNESKEI
                     KDEDVESVVKSALTKSITPEREVISLIPLEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPTTILHNLRKTVERAGIKVEHVVIAPLALAKSVLNEGEREFGATVIDMGGGQTTVAS
                     MRNQELQYTNIYSEGSDYVTKDISKVLRTTVEIAEALKFNFGQANVEEASTSDTVQVN
                     VVGNEEPVEITESYLSQIISGRIRQILEHVKQDLGRGRLLDLPGGIILVGGGAIMPGV
                     VEVAQQIFGTRVKLHVPNQVGIRNPMFANVISIVDYVGMMSEVDIIAQHAVTGDEMLR
                     HKPVDFDYKEKTNTMSTMPYSEPLTSSMEDSNLEPIRARENAQEPTEPKANIGERIRG
                     IFGSMFD"
     gene            complement(26085..27221)
                     /locus_tag="VBE22_000108"
     CDS             complement(26085..27221)
                     /locus_tag="VBE22_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEO7201590.1"
                     /translation="MPKKKSDTPEKEEVVLTEWQKRNLEFLKKRKEDEEEQKRINEKL
                     RLDKRSKLNISSPEEPQNTTKIKKLHFPKISKPKIEKKQKKEKIVNSLAKTNRIRTAP
                     IFVVAFLVILVSVFLLTPFSKQKTITVSGNQHTPDDILIEKTNIQKNDYFFSLIFKHK
                     AIEQRLAAEDVWVKTAQMTYQFPNKFHIQVQENKIIAYAHTKQGYQPVLETGKKADPV
                     NSSELPKHFLTINLDKEDSIKLLIKDLKALDPDLISEIQVISLADSKTTPDLLLLDMH
                     DGNSIRIPLSKFKERLPFYKQIKKNLKEPSIVDMEVGVYTTTSTIESTPVKAEDTKNK
                     STDKTQTQNGQVAENSQGQTNNSNTNQQGQQIATEQAPNPQNVN"
     gene            complement(27225..28301)
                     /locus_tag="VBE22_000109"
     CDS             complement(27225..28301)
                     /locus_tag="VBE22_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEO7201591.1"
                     /translation="MGKKIVFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     EQINQSGLDITFHSIATGKLRRYFSWQNMLDVFKVGVGVLQSIAIIAKLRPQALFSKG
                     GFVSVPPVVAARLLKVPVFVHESDLSMGLANKIAYKFATIMYTTFEQSKDLIKTKHIG
                     AVTKVMDCKKSFENTDLTSIKEAFDPNLKTLLFIGGSAGAKVFNDFITQTPELEEKYN
                     VINISGDSSLNRLKKNLYRVDYVTDLYQPLMNLADVVVTRGGSNTIFELVAMKKLHLI
                     IPLGREASRGDQLENAAYFEEKGYALQLPESELNINTLEKQINLLISNSESYEKNMSQ
                     SSEIKSQDEFYQLLIDDMAKVTKG"
     gene            complement(28304..29659)
                     /locus_tag="VBE22_000110"
     CDS             complement(28304..29659)
                     /locus_tag="VBE22_000110"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEO7201592.1"
                     /translation="MKTITTFENKKVLVLGLARSGEAAARLLAKLGAIVTVNDGKPFD
                     ENPTAQSLLEEGIKVVCGSHPLELLDEDFCYMIKNPGIPYNNPMVKKALEKQIPVLTE
                     VELAYLVSESQLIGITGSNGKTTTTTMIAEVLNAGGQRGLLAGNIGFPASEVVQAADD
                     KDILVMELSSFQLMGVKEFRPHIAVITNLMPTHLDYHGSFEDYVAAKWNIQNQMSSSD
                     FLVLNFNQGISKELAKTTKATIVPFSTTEKVDGAYVQDKQLFYKGENIMLVDDIGVPG
                     SHNVENALATIAVAKLAGISNQVIRETLSNFGGVKHRLQSLGKVHGISFYNDSKSTNI
                     LATQKALSGFDNTKVILIAGGLDRGNEFDELIPDITGLKYMVVLGESASRVKRAAQKA
                     GVTYSDALDVRDAVHKAYEVAQQGDVILLSPANASWDMYKNFEVRGDEFIDTFESLRG
                     E"
     gene            complement(29789..30034)
                     /locus_tag="VBE22_000111"
     CDS             complement(29789..30034)
                     /locus_tag="VBE22_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEO7201593.1"
                     /translation="MFYLIVALLIALYYFFMAPKTVRNTLNAIGLVGLVALLLVLAVM
                     SFIKILQLPGELYIGLIMIPLGYTAFKEILNLSEKKK"
     gene            complement(30079..31920)
                     /gene="typA"
                     /locus_tag="VBE22_000112"
     CDS             complement(30079..31920)
                     /gene="typA"
                     /locus_tag="VBE22_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEO7201594.1"
                     /translation="MTNLRTDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELEER
                     AMDSNDIEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPSEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSMSDDPSDQEKTMAPIFDTIIDHIPAPVDNSEEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAIEPLPVLRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVQCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLADDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            complement(32152..32532)
                     /locus_tag="VBE22_000113"
     CDS             complement(32152..32532)
                     /locus_tag="VBE22_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO7201595.1"
                     /translation="MDMSVILIIVILLAFVAWASWNYWRVRRAAKFLDNESFQKEMSR
                     GQLIDIREAGAFHRKHILGARNIPASQFKVALSALRKDKPVLLYDASRGQSIPRIVLL
                     LRKEGFNQLYVLKDGFNYWTGRVK"
     gene            complement(32544..33512)
                     /locus_tag="VBE22_000114"
     CDS             complement(32544..33512)
                     /locus_tag="VBE22_000114"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEO7201596.1"
                     /translation="MSKKLLGIDLGGTTIKFGILTLEGEVQEKWAIETNTLENGRHIV
                     SDIVESLKHRLSLYGLTKDDFLGIGMGSPGAVDRTSKTVTGAFNLNWADTQEVGSVIE
                     KEVGIPFFIDNDANVAALGERWVGAGANNPDVVFVTLGTGVGGGVIADGNLIHGVAGA
                     GGEIGHMIVDPENGFMCTCGNKGCLETVASATGVVRVARQLAEQYEGSSAIKAAIDNG
                     DTVTSKDIFIAAEDGDKFANSVVERVSRYLGLAAANISNILNPDSVVIGGGVSAAGEF
                     LRSRVEKYFVTFAFPQVKKSTKIKIAELGNDAGIIGAASLANQQAS"
     gene            complement(33509..33718)
                     /locus_tag="VBE22_000115"
     CDS             complement(33509..33718)
                     /locus_tag="VBE22_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEO7201597.1"
                     /translation="MKVLFDVQNLLKKFGIYVYIGKRLYDIEVMKIELQRLYDNGLIS
                     RDDYLKAELILRREHRLELEKENKK"
     gene            33874..34269
                     /locus_tag="VBE22_000116"
     CDS             33874..34269
                     /locus_tag="VBE22_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEO7201598.1"
                     /translation="MIMSTIYFISLCMSFILSYYDIKYQEYPIFLWILFTISTIVLTP
                     ITKVSIVLCLFGILAEVVDINIGSGDFLYLATIGLSLPLHQMLFIIQIGAWLGIIYCL
                     VMRKMKKTIAFLPFLSIAYIIVTSYSLLF"
     gene            complement(34270..34902)
                     /gene="nth"
                     /locus_tag="VBE22_000117"
     CDS             complement(34270..34902)
                     /gene="nth"
                     /locus_tag="VBE22_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEO7201599.1"
                     /translation="MLSKAKSRYIIREIIKLFPDAKPSLDFTNVFELLVAVMLSAQTT
                     DAAVNKVTPALFERFPNPLVLAQADPKEIEPYISKIGLYRNKARFLNQCAKQLIEHFD
                     GKVPQTRQELESLSGVGRKTANVVMSVGFGIPAFAVDTHVTRICKHHQICKQSASPLE
                     IEKRVMEVLPPEEWLAAHQSMIYFGRAICHPKNPKCDQYPQLYHFPDNLK"
     gene            complement(35015..36244)
                     /locus_tag="VBE22_000118"
     CDS             complement(35015..36244)
                     /locus_tag="VBE22_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO7201600.1"
                     /translation="MLESLKTIVKTNSDKKLFDGDLEVSYGEFYNLVRQDMASQDNRK
                     HVISTHSLLNQLVRFVSKLCQKALPIICKPNLTHNEISRLEKEVQYAPQLADFGVLSS
                     GTTADAKLLWRSFASWSDFFSIQNAYFSVTSNSRLFIQGDFSFTGNLNLALSLLLLGG
                     TLVVTQKNSVKYWQTLWEKTGVTHLYLLPSYLKLVEQYSKETALDNKTIITSSQYVSD
                     SLLEGLYIKHPKVSVKIFYGASELNYVSWYDGRDIRDKPQYVGEIVPNVAVRIKEGRI
                     FVKTPYSICGLSSEYCAGDYGELIDGKLYLFGRGGDWCNQSGIKLYLPRLIEKIKTCP
                     YIKDAVAFTKESQSHGQESHCCIVLIENQMEQECLKWLSEHFEKKYGFKHYHIVSKIP
                     LMPSGKIDYQQLKRQLA"
     gene            complement(36237..37352)
                     /locus_tag="VBE22_000119"
     CDS             complement(36237..37352)
                     /locus_tag="VBE22_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEO7201601.1"
                     /translation="MKNRDVYIGFGLRTPIGIKGKQFKHYRPELLGAHLLNQIKKIEP
                     ESNIDSIICGNTVGTGGNIGRLMTLFSDYESYIPVQTIDMQCASSSSALFFGYLKISA
                     DINEKVLVGGIESSSLQPMRRYAKEDNRNGEYTVAQFSPDSYAETVMLEGAQRVCQKY
                     GFRREILDKLAFLSHKRALTAKQGGYLEEVILPMEGMRDQGVRKLKETFFQKLPRLME
                     NSPLLTIGNVCLMHDAAAFLTLQSQKTEFRIVHIVEVAGDPKLSPELVHTATEKLLTE
                     THTKISDYDAIEWNEPFAAIDALFNHYYPEEREKFNIFGGALAYGHPYACSGIINILH
                     LMQALKYKNKPMGLTAIAGAGGVGMAISIEYLGVKNA"
     gene            complement(37342..37857)
                     /locus_tag="VBE22_000120"
     CDS             complement(37342..37857)
                     /locus_tag="VBE22_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201602.1"
                     /translation="MKKLFKKAIAMCRDLPLNFVIVELSLYQREDGSSDNIKWISKRI
                     LEVTVTDQQWIYHLRMLNLPKLKPMSLASSDCPAREEKVSLISIEECQKFTSLVRDKN
                     PFHQGEAAIVPAALLLEKIMEVRKGKIKEIHLTFLSPVRVNQEFSVQVVDNTVIIFQH
                     QILCVRGTYEE"
     gene            37982..38974
                     /gene="bioB"
                     /locus_tag="VBE22_000121"
     CDS             37982..38974
                     /gene="bioB"
                     /locus_tag="VBE22_000121"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEO7201603.1"
                     /translation="MSFQTNYIHLADEILSGKTSISYEQALEILNSDENWWEIYAAAL
                     YLKNQVSRNNIRLNVLLSAKQGLCAENCGYCSQSKESTADIDKFGLLPQNVILKQAIV
                     AHQNGASVFCIAMSGTKPSKREIEQLCQVIPEIKKSLPLEICLTAGFLDREQLHQLKQ
                     AGIDRINHNLNTPEENYPNIATTHSFKDRCDTLERIHNEDIDVCSGFICGMGESDEGL
                     ITLAFRLKELNPYSIPVNFLLAVEGTPLGKYNYLTPIKCLKIMAMLRFVFPFKELRLS
                     AGREVHFENFESLVTLLVDSTFLGNYLTEDGRNQHTDIEFLEKLQLNHTKKELI"
     gene            38975..39514
                     /locus_tag="VBE22_000122"
     CDS             38975..39514
                     /locus_tag="VBE22_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO7201604.1"
                     /translation="MNTRTTTYIALMVALLIVLGFIPGIPLGFIPVPIVLQNLGVMLA
                     GALLGSRKGFLAVAIFLLLVAIGAPFLPGGRSGLVTLFGPTAGYLLTYPFAAFFIGLG
                     LEKVKTTKLWVQFLIIWIFGVLLIDICGSIVLSFQTSLPLTKSLFSNLIFIPGDTLKA
                     SICLIIYRKFANRLTHLYN"
     gene            complement(39581..40147)
                     /locus_tag="VBE22_000123"
     CDS             complement(39581..40147)
                     /locus_tag="VBE22_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEO7201605.1"
                     /translation="MLLLVDNYDSFTYNLKQYLSVYKEIFVIKNDDPNLFLLAESAEA
                     IVLSPGPGHPKDAGKILELINQFIGQKPILGICLGHQALAECLGGRLNLANHVMHGKQ
                     SWVTINDHTSLFKGIDSPTQVMRYHSLVVTDLPENIAIIARSNEDNEIMAFHCPSLKV
                     YAMQFHPESIGSIDGMKMIENFLTLIND"
     gene            complement(40275..42014)
                     /locus_tag="VBE22_000124"
     CDS             complement(40275..42014)
                     /locus_tag="VBE22_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7201606.1"
                     /translation="MKSNQWQVFKRLISYLRPYKWFTVLALSLLLLTTVVKNIIPLIA
                     SHFIDHYLTNVNQTAVLILVGYYSMYVLQTLIQYFGNLFFARVSYSIVRDIRRDAFAN
                     MERLGMSYFDRTPAGSIVSRITNDTEAISDMFSGILSSFISAIFIFTVTLYTMLMLDI
                     KLTGLVALLLPVIFILVNVYRKKSVTVIAKTRSLLSDINSKLSESIEGIRIVQAFGQE
                     ERLKTEFEEINKEHVVYANRSMSLDSLFLRPAMSLLKLLAYAVLMSYFGFTGVKGGLT
                     AGLMYAFIQYVNRLFDPLIELTQNFSTLQTSMVSAGRVFDLIDETGFEPSQKNTEAFV
                     REGNIEFKNVSFSYDGKKQILDNVSFSVKKGETIAFVGATGSGKSSIINVFMRFYEFQ
                     SGQVLLDGKDIRDYSQEQLRKNIGLVLQDPFLYHGTIKSNIKMYQDITDQEVQDAAEF
                     VDADQFIQKLPDKYDSAVSERGSSFSTGQRQLLAFARTVASKPKILILDEATANIDSE
                     TEQIVQDSLAKMRQGRTTIAIAHRLSTIQDANCIYVLDRGKIIESGNHESLLDQKGTY
                     YRMYQLQAGMMEV"
     gene            complement(42004..43749)
                     /locus_tag="VBE22_000125"
     CDS             complement(42004..43749)
                     /locus_tag="VBE22_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7201607.1"
                     /translation="MSIIKNLWWFFKEEKKRYLIGILSLSLVAVLNLIPPKIMGLVID
                     AITTGKLTRPQLLWNLLGLVLSALAMYGLRYIWRMYILGTSYKLGQVVRYRLFEHFTK
                     MSPSFYQKYRTGDLMAHATNDINSLTRLAGGGVMSAVDASITALVTLITMFFTISWQM
                     TLIAVIPLPLMALATSKLGRKTHETFKESQAAFSELNNKVQESVSGVKVTKSFGYQEQ
                     EIASFQEVNQMTFVKNMRTMTYDVMFDPLVLLFIGASYVLTLAMGAFMISKGQVTVGD
                     LVTFVTYLDMLVWPLMAIGFLFNMVQRGSVSYNRINSLLEQESDITDPLNPIKPVVNG
                     TLRYDIDFFRYDNEETLADIHFTLEKGQTLGLVGQTGSGKTSLIKLLLREHDVTQGKI
                     TLNKHDIRDYRLSELRQLIGYVPQDQFLFATSILENVRFGNPTLSINAVKKATKLAHV
                     YDDIEQMPAGFETLIGEKGVSLSGGQKQRIAMSRAMILDPDILILDDSLSAVDAKTEH
                     AIIENLKTNRQGKSTIISAHRLSAVVHADLILVMQDGRVIERGQHQELLNKGGWYAET
                     YASQQLEMEEAFDEV"
     gene            complement(43945..44460)
                     /locus_tag="VBE22_000126"
     CDS             complement(43945..44460)
                     /locus_tag="VBE22_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEO7201608.1"
                     /translation="MLKRLLTEDGELTKISRRFVWMLVIIYCLIIVRMCFGPQIMIEG
                     VSTPNVQRFGRIVALLVPFNSFRSLDQLTSFKEIFWVIGQNVVNILLLFPLIIGLLSL
                     KPSLRKYKSVILLAFLMSLFIECTQVVLDILIDANRVFEIDDLWTNTLGGPFALWTYR
                     NIKGWLLTIRK"
     gene            complement(44460..45566)
                     /gene="rlmN"
                     /locus_tag="VBE22_000127"
     CDS             complement(44460..45566)
                     /gene="rlmN"
                     /locus_tag="VBE22_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEO7201609.1"
                     /translation="MPKKTDTPDYKPSIYSLTRDELIAWAIEHGEKKFRASQIWDWLY
                     KKRVQSFDEMTNISKDFIALLNENFVVNPLKQRIVQESADGTVKYLFELPDGMLIETV
                     LMRQHYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEITAQIMLVQKYFDERGQG
                     ERVSHIVVMGIGEPFDNYTNVLKFLRTVNDDNGLAIGARHITVSTSGLAHKIREFANE
                     GVQVNLAVSLHAPNNDLRSSIMRINRSFPLEKLFAAIEYYIETTNRRVTFEYIMLNGV
                     NDTPENAQELADLTKKIRKLSYVNLIPYNPVSEHDQYSRSPKERVEAFYDVLKKNGVN
                     CVVRQEHGTDIDAACGQLRSNTMKRDRQKAKVGQ"
     gene            complement(45617..46195)
                     /locus_tag="VBE22_000128"
     CDS             complement(45617..46195)
                     /locus_tag="VBE22_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEO7201610.1"
                     /translation="MRKEVTPEMLNYNKYPGPQFIHFENIVKSDDIEFQLVINEKSAF
                     DVTVFGQRFSEILLKYDFIVGDWGNEQLRLRGFYKDASTIRKNSRISRLEDYIKEYCN
                     FGCAYFVLENPNPRDIKFDDERPHKRRKSRSKSQSSKSQTRNNRSQSNANAHFTSKKR
                     KETKRRQERHIKEEQDKEMTSAKQHFVIRKKK"
     gene            complement(46265..47599)
                     /locus_tag="VBE22_000129"
     CDS             complement(46265..47599)
                     /locus_tag="VBE22_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO7201611.1"
                     /translation="MTELIRILHLNDLHSHFENFPKVKRFFHDNQAQPIETISLDLGD
                     NIDKSHPLTEASSGKANIQLMNELGIELATIGNNEGVGLSKKDLDQVYKDSDFTVIVG
                     NLKDNTIEPSWAKPYIIYETQQGTKLAFLAYTFPYYKTYEPNGWMIEDPIDCLKCHLQ
                     INEIKEANCRILMSHLGIRFDTRIAQEFSEIDLIIGAHTHHLFEEGELINGTYLAAAG
                     KYGRFVGSIDITFDNHTLKDILISTCDTKQLTGYPSDSDWAKKIESEGKEQLRKESLI
                     SFPAPLSLEESCQLVMDAMLFYAGADVAIINSGLIVQPFRKDFSRKNLHESLPHQMRL
                     AKLTVSSQELLEIYETIYQQGQFLAQQKIHGMGFRGKYFGDVLHSGFDYKNGKIVYNE
                     KDIDAKEEVILVIVDQYYFASYFECLKTKKVDLLFPELLRDVVADYLINMTL"
     gene            complement(47674..48747)
                     /locus_tag="VBE22_000130"
     CDS             complement(47674..48747)
                     /locus_tag="VBE22_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEO7201612.1"
                     /translation="MKNKDPKRKHKSLLGLLKWWIIGFAFLLLVLASLVVRLPYYLEM
                     PGGAYDIRSVLKVNKKADKAKGSYNFVAVSVSQATPAQVFYAWLTPFTELSSKEETTG
                     GFSNDDYLRINQFYMETSQNESVYQALKLANKQVSLTYKGVYVLNLAKNSTFKDRLHL
                     ADTVTGVNGKSFKNSSQLIKYVAALHLGDKVKVQYTSQGKKKESVGKVIKLSNGKNGI
                     GIGLTDHTEVSSDVPVDFNTEGVGGPSAGLMFTLAIYDQLVKEDLRKGRKIAGTGTIE
                     QNGHVGDIGGAGLKVVSAAKKGMDIFFVPNNPIDKNAKKGKTKVQTNYQEAKAAAKRL
                     GTKMKIVPVQNVQQAIDYLKKTK"
     gene            complement(48737..49222)
                     /gene="coaD"
                     /locus_tag="VBE22_000131"
     CDS             complement(48737..49222)
                     /gene="coaD"
                     /locus_tag="VBE22_000131"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEO7201613.1"
                     /translation="MTKKALFTGSFDPVTNGHLDIIERASYLFDHVYIGLFYNLEKQG
                     YFSIECRKKMLEEAIRQFKNVSVLVAQDRLAVDLAREVGAKYFVRGLRNSQDFDYEAN
                     LEFFNKQLADDIETVYLSTSPSLSPISSSRIRELIHFKASVKPFVPKSVVREVEKMSE
                     E"
     gene            complement(49219..49509)
                     /locus_tag="VBE22_000132"
     CDS             complement(49219..49509)
                     /locus_tag="VBE22_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201614.1"
                     /translation="MDYKLVMIILILVILIYLLLRLHYLEELLKHYHFKNDEGVCSLS
                     RTKNPLYLLMAMITFLPNLINWIANGFEAIDIVRLIPVVVFLWLYYCGDDDE"
     gene            complement(49521..50225)
                     /gene="rsmD"
                     /locus_tag="VBE22_000133"
     CDS             complement(49521..50225)
                     /gene="rsmD"
                     /locus_tag="VBE22_000133"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEO7201615.1"
                     /translation="MAIKLNNNFLIIMGTSFLLLLAYHIKKEGKEIPSSLFVISNFFD
                     NSTLFFVIIVVMRVVAGTFGGRPLKTLDGKTTRPTTDKVKGAIFNMIGPFFEGGRVLD
                     LFSGSGSLAIEAISRGMDQAVLVEKDRRAQVVIQENIAMTKSPEQFQLLKMEANRALE
                     QLTGQFDLVLLDPPYAKEEIVKQIQIMDSKGLLGDDIMIVCETDKSVDLPEEIASFGI
                     WKQKIYGISKVTVYVR"
     gene            50191..50640
                     /locus_tag="VBE22_000134"
     CDS             50191..50640
                     /locus_tag="VBE22_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001893380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEO7201616.1"
                     /translation="MIKKLLLSLIAILCLTTLKSNLSSADTMTISPQQYQHMITNSDL
                     IAITDKALVSKSQQSEATIFIGRPTCQYCRAFLPKLLKSQATLHSKIYYLDSQKYKGE
                     RLKSFFKKHHITTVPNLAHYQRGKMTKYLVQGSQATPQQIQTFLSQK"
     gene            complement(50685..51677)
                     /locus_tag="VBE22_000135"
     CDS             complement(50685..51677)
                     /locus_tag="VBE22_000135"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEO7201617.1"
                     /translation="MKKSFIHQQQEISFVKNTFTQYLIDKLEIVEVQGPILSQVGDGM
                     QDNLSGIEHPVSVKVLNIPEAEFEVVHSLAKWKRHTLARFGFNEGEGLFVHMKALRPD
                     EDSLDPTHSVYVDQWDWEKVIPDGRRNLDYLKETVEKIYKAIRLTELAVEARFDIESI
                     LPKRITFIHTEELVEKYPDLSPKERENAIAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTTPSENGFKGLNGDILVWNEQLGTAFELSSMGIRVDEDALKRQVVLTGDEDRLEF
                     EWHKTLLRGFFPLTIGGGIGQSRLAMFLLRKKHIGEVQSSVWPKEVRDTFENIL"
     gene            complement(51790..52854)
                     /locus_tag="VBE22_000136"
     CDS             complement(51790..52854)
                     /locus_tag="VBE22_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEO7201618.1"
                     /translation="MSIESDFFRKKRFIFSSLEEFGFIKSDQEYIYCQTFMDNDFKAI
                     ITISLDGKIAGKVIDSALEEEYLPLRAANYNGSFVGEVRSAYMAILGDISDSCCKDLL
                     FTKDQSNRLAEKIAKTFEDSVDYPFAKHPQYASYRVSGKWYALLFPLKMGKLENVPAQ
                     LSEDEVEVLNIKVNPQDMEILLQKEGIYPSYHMSKKTWVSIVLDNTLSDIEIFKLVSD
                     SRKLVSPNKKSNSEPEFWIIPANPKFYDIDKEFAEHQLIDWTQKGKIAVGDYVFIYIT
                     APIRALRYVCQVIERDLKTSTYLGARDVKAYMRLKLIRQFEDSTYPVAFLAEHGVKTV
                     RGPRRMTKELRQVMAKTILE"
     gene            complement(53014..53916)
                     /locus_tag="VBE22_000137"
     CDS             complement(53014..53916)
                     /locus_tag="VBE22_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO7201619.1"
                     /translation="MFSSDFGFEIINVDDLSLQEERFLPSELLAYARDENESSFVRDI
                     EGHLTLVYQLLDTQGHVDDVRHVPRVIPVTLFLKEDGLFVLANHKNINLVKKALNRVE
                     KVDSPKHLLLSLVTAFSKQYFDVLDTISEERDKLINDLRKRPNKSNLARLANLQSGTV
                     HLMMGTKQNFEMLTDLQNIEQDKENTRNEKMQLQDAIIEARQLSNMCSLNSQVFQELS
                     SSYNNVLSNNLNDNVTTLTIISIGISIIAMVTSFYGMNVKLPFDSVDAVWVLIILITT
                     IITIMLSIVMYIYVHKEDNHPKHK"
     gene            complement(54077..54523)
                     /locus_tag="VBE22_000138"
                     /pseudo
     CDS             complement(54077..54523)
                     /locus_tag="VBE22_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
     repeat_region   54673..55757
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=54737..54768
                     /rpt_unit_seq="atttcaatccacgcacccgcaaagggtgcgac"
     gene            complement(55904..56197)
                     /gene="cas2"
                     /locus_tag="VBE22_000139"
     CDS             complement(55904..56197)
                     /gene="cas2"
                     /locus_tag="VBE22_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEO7201620.1"
                     /translation="MMVLVTYDVNTKTVAGRRRLRHVAKLCVDYGQRVQNSVFECSVT
                     PAEFVEIKNELLTIIDQKSDSIRFYLLGKNWQNRVETIGKNDSYDPDIGVLLL"
     gene            complement(56208..57233)
                     /gene="cas1c"
                     /locus_tag="VBE22_000140"
     CDS             complement(56208..57233)
                     /gene="cas1c"
                     /locus_tag="VBE22_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO7201621.1"
                     /translation="MKKLLNTLYLTQDNFYLTRERDNIVIKQEGKIVHRFPYRIIDGI
                     ICFSYLGASPSLIELCTEKQINLSFHTPQGRFCGRFVGSTNGNVLLRREHYRLADHAL
                     SIEYAKRFILAKVSNSRKYLIRFKRDHRDRIDIQLFEDINTELMWALELIQSAPDKET
                     LLGIEGQAANQYFRAFNELVLTDKDTFRFNGRTRRPPQDCINALMSLGYSLLTYECQS
                     ALEAVGLDSYVGFYHTDRPGRASLALDLVEEFRSYIVDRFVFSLVNKGQLNKRHFDIK
                     ENSSVILTEKGRTIFIESWQKRKHTVVEHPFTKEKVKLMLLPYVQAQLLSKAIRGDLE
                     SYPPFLI"
     gene            complement(57230..57901)
                     /gene="cas4"
                     /locus_tag="VBE22_000141"
     CDS             complement(57230..57901)
                     /gene="cas4"
                     /locus_tag="VBE22_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEO7201622.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQI
                     LHTKADNPYIKEKRKDFLVSRAMHVSSKILGLYGILDVVEFYKDENGFSLKGKRGKWL
                     PCIVEYKRGKPKKDRRDIVQLVAQTICLEETFGCYIEKGCLYYHSVNQKKTIEITEEL
                     RQEVLDLAEQMHFYYDNKIIGKAEYFKNCPLCSLVDICKPRLSKKTRNVDNYIRQSLV
                     NEDSL"
     gene            complement(57903..58751)
                     /gene="cas7c"
                     /locus_tag="VBE22_000142"
     CDS             complement(57903..58751)
                     /gene="cas7c"
                     /locus_tag="VBE22_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HEO7201623.1"
                     /translation="MLEHKIDFIVTFEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKPIFVQANERIEDDFRSLEKRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPISISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGIYVLKGSINAYFAEKTGFSQEDAEAIKEVLVSLFENDASSARPEGSMRV
                     REVFWFTHSSKLGNVSSARVFDLLEYNQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTV
                     KILEGL"
     gene            complement(58756..60651)
                     /gene="cas8c"
                     /locus_tag="VBE22_000143"
     CDS             complement(58756..60651)
                     /gene="cas8c"
                     /locus_tag="VBE22_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEO7201624.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRNNEPVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMTDKQMIIFPVTADSVARSGSNPAPHPLVDKFAYYSAEMG
                     QSQYDSFHKQLNNWIDYCEEGDVKRFLTIVQQFILKPEFLTLILDSLIGPDYQNNQLK
                     VTFCDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKTLHQSYISFVEANRENLGI
                     CNISGREEQLTDKHRGLMGNAKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLMI
                     KYLLENKNTSTWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEDDDEDMPSVIAL
                     ATEENKRIGKSFIKGQKLFANDATYYIAILNKTSNGRIALKYFRQIQASQLLTNLDKW
                     QEKYSWESRSKSGKSRLRTPAFHDILNVSYGVDRDRFLELDNDNFKSDQVQKLVTSLI
                     DGKPMPQSIVKKLGNNVKERHRYRKHWYQVEQVCLAILHKQNGEEFSPMLNHTNQNRS
                     YLFGRLLAIFELIETLRYSLEGNGSDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     TLKLNRRGSWKKLEKEKEEILELLNPLLETETMEKPLDYRFIFGYYAEKNYYYPKQNT
                     EVTESEE"
     gene            complement(60651..61379)
                     /gene="cas5c"
                     /locus_tag="VBE22_000144"
     CDS             complement(60651..61379)
                     /gene="cas5c"
                     /locus_tag="VBE22_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HEO7201625.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPATKGGSERASYSVPTRQALNGIV
                     DAIYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFI
                     WNEDRKDLNSDRLPAKHEAIMERSIRKGGRRDVFLGTRESVGLVDDISQEEYEAAVSY
                     YNGVNIDLGIMFHSFAYPKDKKTPLKSYFTKTVMENGVITFKTQSDCDIVNTLSSYAF
                     KVPGEIKSVNDEYMEYDAMEKGEN"
     gene            complement(61512..63935)
                     /locus_tag="VBE22_000145"
     CDS             complement(61512..63935)
                     /locus_tag="VBE22_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HEO7201626.1"
                     /translation="MMTKETLAHYQKKIEQESEKKQSLDEHLWHVACFSRQEASIIGQ
                     GDVLFLISLYHDLGKADRAFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKSRGVDKD
                     ERMTFNEMVGYVISAHHGMYDFCYCSDDAEYYSFNKFKNRINRDLDDYHYHEDIKGYA
                     IKLEEKLCDYGYKDLRELIDKAFDNYQQAMSSLNWQDNSEWDYYQSCMVRLYLSLLKN
                     ADILDTVNAYGLEISPMDKTERSSLKHSYLAAIEQKYASFGRPNNQLNTIRTEIAERV
                     KERGKRDSKGIYRLDLPTGAGKTNLSMRYAFHQLVHQDKSRFFYITPFLSVLEQNASE
                     IRKVTGDLGVLEHHSNMVKQANEDDDKDSLLSAYLIDSWDSQVVLTSMVQFFQTLFKT
                     KSANLRRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFLNKVMDTTIVLCTATQPAYD
                     SSEIDHRICYGGNLGELAEIVELTIEEKQIFSRTELRKFDDSDQKVYLTDVINLILGE
                     ENSVLAIFNTKKTVHNCYTMLKDMTDRPIYQLSTNMCAQHRLDLVAKIKTELQNNTPI
                     ICISTQLIEAGVDVDFHRVIRSYSGIDSIVQAAGRCNREGKRDKGQVTLVNLTNEEEN
                     ISRLTEIKAKKEATESILYKIGSPINISTLNRDFFEYYYANNQGLMDYPLEDNISIYD
                     YLSLNTYQTANKKFKGKLKQAFKTAGAKMNLINNDMIGILVPYGDAEKKLAYLEELGT
                     SHFLSAEDYQTIKSLLKELQPFTVNVRENDPLLEATKSYLNGQILVLTSEYYDTERGV
                     KYDSASFYF"
BASE COUNT        20380 a        13022 c        10080 g        21220 t
ORIGIN      
        1 ctttttctgg aagcggaaga gttcctactg aaaatgaggt cactttaagt tgaacagcgc
       61 catttgttaa acgataaggt tcaaaataga tgtataaagg cacttcataa cctaataatt
      121 gataagagcc ttcaaatagt atagaagatg aagcagcata aatcttataa ttcatcttct
      181 tagtttgata ttgttttaaa taaagtgcaa ttgtcttatt cagttgcgac ttattggtcg
      241 taaaagtacc tacttttact ttatcttgta ccccagtcga aatttttcct gtattaggct
      301 cacgtacttg aattaagcga cttgcaatca ctgctgtgaa ggaaaggtta attgccaaca
      361 ataagagaaa tgcccatttc cagaaattta aattacggcc agttttttct tgtttcattt
      421 attttctcca taacggcgtt agacatgatt tgatagccaa tattattggg atgaaaatgg
      481 tctccagtaa agagagcatc attagtgata cttgcctgac tatttgatga ctctgtaata
      541 ccctctttac catttattcc cttataaagg cggtcattaa ttgggacaaa ataaacattt
      601 tctgaagcat caactacttc ttttgtagct ttattccaat tatcaataac ggtttgcatt
      661 ttagttaatt gtggaaagtt taggtaaaaa ggattataaa tgcctaaaac ataaataggc
      721 aatttaggat tatcttgtct tgcttttgca agaatttctt tcaaacgttc cttatatgct
      781 tctgctggtt tctcaaagga atttagtgat aaatgactga gctctttacg aataacagcc
      841 aagacatcat taccaccaac agttagcgtc aataaatcag ctttctctaa atctttttcg
      901 atttgaggat ctgtcgtcat acgttttaaa atttgttgac tagtattccc agacacacca
      961 taattaacag aagtcacttg gtaagagtat cgattatgga gtgattctga tagcagtggg
     1021 acaaaaccac cttgagaggt tgtatccccc acaccttcgg tcagagaatc tccaagagca
     1081 acatagttaa gtgggataac tttctttgtt aggaagtctt tttttgttaa tttaggattt
     1141 gattttggaa tgatcaagga aaaaatccca aaagataaaa gaagacttac cagaaaaaaa
     1201 gaaagtccgg taagtatttt tttcttattc ataacgaacc ataacagcaa atgcatcctc
     1261 accagtatga gtttggatga ttgagccagt ttctaatacg gatataggag cacctagaac
     1321 agctagtttt tctttaaagt tattagccat atcagcttta ccacagtagg agatgccaat
     1381 ttcagcaatt tttcgtgttt gggcactttc aacaaaatta tctaaccatt tgctgaaggt
     1441 ctttagaccg cgacctttaa caatcggtac taattcatgg tttgttagct ccataattac
     1501 ttttatgtta agcaatgaac ttagaagacc cgttacacgt ccaatacgac caccttttac
     1561 taaattttca agggtcgata cgccaataaa taattctgac ttctggcgta cttcttccac
     1621 acgagccaaa attgtatcta aatcagctcc ctctttagct aatttcgcgg cttctacaac
     1681 ctggaatttt tgacactggt ctgtaaaagt agaatcaata actgtaacat ctgcaccagc
     1741 aatattagct ccctggcgtg atgcttcaat agttcctgat agtgtatgcg ttaaatgaat
     1801 tgcaatgata tgttccgcac cttcattcat taatttttca taaatttctg caaaaacacc
     1861 tacaggtggt tgacttgttt ttggcaattc cttacttcca cgcattaggt tcaagaattc
     1921 cccttgtgct tttaagtcat tgtctgaata caaggtacca tctatcataa ctgacaaggg
     1981 aacaactgta atgtcaagct ctttaataag ttctggttca atggtaatgg aagaatccgt
     2041 tacaattttg attttactca tatcctatcc tattctatac tttaatattt caataccctt
     2101 attataacaa aatttaagtg tatcaacatt aatttctcct aacaatgtta gctttttaaa
     2161 gacgttacca aagctaacta ccaagtaatt ctttagcttg agtacgtgca gtgtctgtta
     2221 cattgtttcc tgccaacatt ttggcgattt cttctacacg ttcttcatag cttaaaagcc
     2281 ttaccgttga aactgttgaa gagtcactgg aaattttttc aataaaatat tggtaatccg
     2341 ctattgcaat aacctgagct aaatgagaaa ttgctaaaac ttgtccatga ctaccaatct
     2401 tgtgaatttt ttgagcgata gcctgggcca cgcgaccaga aactcctgta tccacctcat
     2461 caaatacaat ggaagtttta tcttccttac gtgaaaaagc agatttaata gctaacatca
     2521 atcgagataa ctctcctcct gaagccactt ttaccaatgg cttaaagccc tctccgggat
     2581 tcgttgaaat ataaaattca acgatttcgt tcccttcttt attaaatttt ccttttgtaa
     2641 attgaacttg gaaatcagct ttctccatat aaagctctgt taattcttgt ttgatttcat
     2701 tttccaattg tttagctagc ttgtgacgct ctaaacttaa ttgatttgct gattcaatca
     2761 aatcgtgttc taaaatcttt aattcttttt ctaaagcatc tgaggactct tcactacctg
     2821 tcaatagact atattcttta gtaatgttat caaaatagtc taaaacatca ttcacatcac
     2881 cgccatattt tcgcgttatt gtgttaataa catctagtct attttcaatt tcttgtagta
     2941 aaccagcgtc aaaatctaaa tcatcgataa cgtcacctaa acgtttagta acttcttcaa
     3001 taacgtagta agcttctgaa agattggtgg aaagatcttt atattctcga tcaaattctt
     3061 ctaaagccat aaggtcattc attgcagaac gaacattcga taaacttgaa aactcttcgt
     3121 tatctaacat aagatatgca tttgtcaaag tatctgcaat attcttatgg ttcattaatt
     3181 tatcacgttg cttgagtagc gtttggtctt catctgattt aagggctaca gactcaattt
     3241 ctgctatttg aaattctagc atttcaatac gtgatttatt ctcttgttca tttttttgct
     3301 tatccaatac gcgtttacga agctgacgat aagcatcaaa aagactttga taacgttctt
     3361 ttatgacatt aaattctgta ttaccaaatt catctaacat cagaatgtgc atgttaggtt
     3421 tcattagttc ctcttggtca tgctgaccat gaatgtctac caaataatgc cccacagctt
     3481 ttagtgttga caaattcacc atttgcccat taattcgact aacgctgcgc ccattttgaa
     3541 agatttctcg gcgaataatt aattcatctg ctaattcaat gccattttct tccaataatt
     3601 ggactaatga ttgatttttt tcaacagaga aaaatccttc aatttctgct ttgttagcac
     3661 catggcgaat cacttcaaca ctagcacggg ctcctaacat catattcata gcatcaataa
     3721 tgatagattt ccctgcacca gtctcaccag ttaaaacagt cattcctgtt tcaaaattaa
     3781 gtgaaatttc ttcgataata gcgaaattct taatagaaat ttctaaaagc ataaggtcct
     3841 ctactaatct tttttctcca acattgcaat cattgattga cgaatgtgat cagcatctgc
     3901 ttctgatttt gcaatcaaaa ggagactatt atcgtcagct gtcaaactaa aaatatgttc
     3961 ttgacaatgt gacattatga aggttttaat taattgactg ttacctggta tgacattgat
     4021 attgataaat tgctctaaac ctagtagctt atctgatatt gataaaatac ttgttttaat
     4081 aggctttgcc acagcagttg taaagatagg gtcgttttca tttgacaaac cgtaaatata
     4141 gcgacctttt gctgatggca cttttataat gccaatttca ttcatatcac gtgaaatagt
     4201 tgcttgagtt agagttaccc cataagcttc caatctgcgt aacaactcat gttgtgtttc
     4261 aacagcatgg ttaagaacaa tttgcttaat taaatttaaa cgttcactct tcttcatttt
     4321 tcttaaattc cttatgtgct ttttctataa catcttgtat ttggtcaagc acaagatttt
     4381 gtggatcttg acacttttgc aaatgcatta aaaactcaat atttccatgt ccaccttgaa
     4441 cgggcgaaaa atcaagatgt ttaaccgtat atccataatc tttcgtgaaa ttggtcactg
     4501 ttgtcaaaac cttttcatga accaacttgt ctttgacaat accattttta ccaatttgct
     4561 cacgacctgc ttcaaattgt ggtttaatta atgccactac ttgtccacca tccactaaaa
     4621 tttcttttag agctggtaaa atcaaattaa gagagataaa tgagacatct atcgatgcaa
     4681 attcaggcag tccctccttg aaatcttctt tttgggcata cctaaaatta tattgttcca
     4741 tagaacgaac acgatgatcc tgacgtaact tccaaactaa ttgatttgtt cctacatcta
     4801 ctgcgtaaac taaacgcgct cctgattgta gcataacatc agtaaaacca cccgtagagg
     4861 cgccaatatc tatagttagc ttatctgcaa ctgaaatttc aaaaacttgt aaagcttttt
     4921 ctaatttcaa tccacctcta ctaacatatt ttagtttttc accttttagt tttaattcag
     4981 tatcgtctgc aaccttttcg cctggtttat cataacgttc tccattgata acgttaatca
     5041 ccagtcctgc cataacacca cgtttcgctt gctctcgtgt atcaaaaagt ccctgtttat
     5101 aggcaagaac atctaccctc tctttagcca ttcaaccgta atccctctat tatatctatt
     5161 atttttctag cattaaagtt agcttttttc tctagatttt gaaaaattgc ctgagctttt
     5221 ttcaaagtat cgtcaagtat ttcctgtgac ttatccaaac ccaataaatt tggataagtt
     5281 gtcttttctg ctacaatgtc tttatttggt gtcttaccca attcttcaaa actagcagtc
     5341 acatctaaaa tatcatcacg tacttgaaaa gcatgaccga ttaaaaggcc agcttgatgc
     5401 agttttcctt ttacttcttc cgacttctca gctaaaatac ctgctgcaac aaagggataa
     5461 gctaacaggc gtcccgtttt attaatatga atcaagctta aatcgtcaat agataaaact
     5521 ttgttttctc ccttcatatc taacatttgt ccaccaacca tgccaaaact acccgcagac
     5581 atagacaata actctattaa cctaacagta acatcagctt taaaaccagc ttttacaact
     5641 aaatcaaatg ggtctaaaaa aagggaatct ccagctaaaa ccgctgtagc ttcatcaaac
     5701 tttttatggt ttgttaagcg gccacgacga aaatcatcat tatccatcgc cggtaaatca
     5761 tcatgaatta aagagccggt gtgaatcatc tccaaagcag cagccacatc ataatgacca
     5821 tctattagtt caactccaaa tccctctaaa atttctaaaa ggatgcgtgg acgaatacgt
     5881 ttcccacctc catcaactga gtagaggata gccttaatca aatcaggaga aacgacacta
     5941 tgtgtctgtt tgtaataacg atgaatagct tcatcaattt tttcaatagt aaccatcaag
     6001 tatccatttc tacttccgtt ccgtcagctt gcatcacttt tacaagcgtc tcttcagctt
     6061 cttttaaagt tttttgtaac tcttttgaaa taagcattcc tttttgaaat tcagcaatgg
     6121 catcttctaa ggcaacatca cctgtttcta aacgtgagac aattgtctct aattcctgta
     6181 aattttcttc aaatgttttc ttgtctgaca tccttcacct caacttctaa aagaccatct
     6241 tgcatcttaa cttgcaaatg atctccttca tttattccat tagtagtaga tactaaagta
     6301 tggttttttt cgataatagc gtaaccacgc gctacaattc tagaactatc caatgatata
     6361 agcgcatctt gagccttttc aaaacgtgct agcttgctgt catactgact agtcattgta
     6421 cttgataata gtcgtctact ttgtacaaca cgctcttggt aagtatgaat acgctgtttt
     6481 aaatctatgc cttgcagttt ttgatgaagc aaaccttgct tttgtcttac tgtttgtagt
     6541 ttatcacgca tagaaaatgt taactgctga tttaggttat ctaatttttg aaggaaacca
     6601 tcatatagac gctctggttg cctaaaaata actgattgct ttgatttttg caacctctcc
     6661 tcttttgtca tgataagacg aaggctagac tgatacattc tattttcacg ttcagtaatc
     6721 caagacaaga tatcaatttt agtaaccggt gtagcaagct cagctgctgc agtcggagtt
     6781 gctgctctgc gatcggctac aaaatctgct aaagttgtat cagtttcatg tccgacacta
     6841 gaaataacgg gaagacgaga ttcaaaaata gcttctacaa cacattcttc attaaatgcc
     6901 cataagtcct caatagaacc tcccccacgg ccaacaatta atagatccaa atcctttttc
     6961 tcgttagcta aagcaatagt ttgtgctatc tcctgagctg cgccctctcc ttgaactttt
     7021 gtggggaaaa gtaatatttc cactcctggg aatcgacgag atactgttgt aataatatcc
     7081 cgaataacgg ctccacttgg actagtcaca actcctattt ttctaacaaa ttgaggaatt
     7141 aattgcttat gacgatcatc aaaataacct gcttgagaga gtttcttctt tagttgttca
     7201 aattggatag caagagctcc tataccatcc ggttctgctt tttcaacaat aatagagtag
     7261 gagccgcttg gttcataaag ttgaacacgg ccaactacat taaccttcat cccttcctct
     7321 aattcaaaac ccagtttttt gaattgacct gaccacattg tcgcctgaat gactgattta
     7381 tcatctttta aagagaaata ttgatggttt gggcgacgac gaaaattaga cacttgccca
     7441 gttaaataaa ccctctctaa ataagggtct ttgtcaaatt ttaattttaa atacttagtc
     7501 agtgtcgaca cggataaata gtctgacata gcacctcctc atgagagttt tattaacttc
     7561 attataccaa aaaaacttaa aaaataccta tttgctctac aaatagctcg ataaagcaaa
     7621 agaagtgtca tgctaacacc tctctactta ttttttattt agatagtcta gccatttctt
     7681 ttggaatctc atcactaatc gttgtcggta gaacaacgta ggcttcctta gataattctt
     7741 gagcaattgc cgaatgtaga taaactccca ctgatacctt atctagagga gaggcttctt
     7801 taaattgcgc taacatacct gcaatcatac cacacaaagt atcccccatc cctccagtcg
     7861 cctggtaagg acctcctact ataatttctt tttcgtctaa atcttgaaaa atacgcgtat
     7921 gcgaactctt agcgactaag atcgttcctt tgggaaaaga cttaagagcg gtttgggtat
     7981 tttcttttgt ctgttgcgat acagcaatac ctgacaatcg ctcccattct ttttgatgag
     8041 gtgtcaacac taaattttta gcctttgttt gcggaaacgc accttctgat aacagagtca
     8101 aggctgatcc atcaatgatg acactttgat gtgattgaat cttctccatg gtctggttca
     8161 agatttttcg agatgattct gatactccta atccaggacc aattacaata acatcacttt
     8221 gaacaattga tttttccaac catttgtaat catcactatc aaacgccatt acctctggca
     8281 gttgactatg caaagagggg atattgcaac tttgggttgc tacagtaact aatcctgcac
     8341 cagttttgac acaggccaaa gctgccatta taatagcacc tccataggga taaaaacctc
     8401 ctatcagaag aacactacca taatcacctt tatggcttga tttagggcgt ggcttaatta
     8461 acgccctcgc ttcttgttca ccaacaatca tagactcaca cttctaagag ctgcttgata
     8521 agtttgttcc aaaagcattg taatcgtcat aggtcctacg cccccaggaa caggtgttat
     8581 catactagca acttctgcca cttgttcaaa tgcaacgtct ccaatcaatt taccattttc
     8641 atcgcgattc ataccaacat caatcaccac agcaccttct ttaacgaagt cttttgtaac
     8701 aaagtgcccc tgaccaatcg caacaataag gatatcagcc tcctttgtta cctcagaaag
     8761 gtttcgagtt cttgaatgtg tcaaagtaac tgttgcgttt ttatcaagga gaagttgtgc
     8821 cattggctta ccaacaatat tggaccttcc gatgataaca gcatgttttc cctctaaatc
     8881 cacatggtac tctctaaaca tctccataat accagctggc gtacaaggta ccatcatcgg
     8941 tcgtcctgac caaagatgac ccgtattcat tgggtggaaa ccatcaacat ctttcttagg
     9001 atctattgct aaaataattt ttttatcgtt aatatgttgt ggtaacggaa gttgaactaa
     9061 aataccatgg atacttttat cttcattgta ttgatggata atatcaatta gttcttcttg
     9121 agaaatggat tcagataatc gtagagtctc acttttaaaa ccagcttcta atgcagagcg
     9181 ttctttattt cgaacgtaca cttggctagc tggattatcc ccaacaagga ttacagctaa
     9241 accaggtatg ataccatgct gttcctttaa cctttcaact tttcgcccta actcagcctg
     9301 cattttttgt gataaggctt taccatctat taattcagtc attgatactc cttcttcatc
     9361 cgttttttct atattatatc aaaaaatagc gattatgcta ttttatatcg ctattcattt
     9421 tgtatcaatt ttaatcaata gaaaaggcaa tgaatccctc atcgcctttt tacatatgac
     9481 ttatttagta ctattcattt tagcacgaca agccgactca attgcattaa gtttcgataa
     9541 actatctgct tctgtacaac caatcgtata aaggtaacat tttatcttag gttccgtccc
     9601 tgacggcctt aaagcatacc atgaaccctc attaaaataa tattttaaac aattttgctt
     9661 tggaaaatcc ttataaccat ccttgaaatc aatagaattc tccaatgtca tctcacctac
     9721 ttgtaatatt gggtcctgtc taaaatcctc cataatacga ctaatacgtt cttgcccctc
     9781 agcaccctct aactcaagag aaaattggcg ctcgttgtaa tagccaaatt catcgtaaat
     9841 ggtttgcaaa acgtctaaaa gtgtttgccc tcgttcttta taataggcag tcatttctac
     9901 taccatcatt gaagcactca cagcatcttt atcacgtaca aaagtgccat agcaaaaacc
     9961 aatactttct tcatagccaa agagataagt tttatccttt gagatatcat attcattagc
    10021 ttttccacaa atatttttaa atccggttaa agtttcaaca gtttcaatat tatatttatc
    10081 tgcaataact tttgatagat caccagttac aatggatttt accaatacag gatgatgtgg
    10141 caaattccct aaggcacatc gttgtgaaaa aatataatag gaaagaagtg cccctatctt
    10201 attaccattt aagaaaatat attctccttt actatctttg acttccaatg ctactcggtc
    10261 acaatctgga tctgtggcaa gtaagatatc tgcatcaact gactttccta gagattctga
    10321 ataggcaaat gctttaggaa cttcagggtt tggatagcca accgttgtaa aatcaggatc
    10381 gggcatttcc tgctcaggta ccacataaac attttcaaaa ccacggcgtc ttaaaacttc
    10441 gcgcacaggt aaatttccta cgccatttaa aggagtataa actactcgga ctgacttatc
    10501 aatgttagta tcattaatag ttaaaccaag cacttctttt ttatatgctt cttcaatact
    10561 ctcatcaata taacttgcca aaccacttgc cagagcctct tcaaagggta tttgcttaat
    10621 ttgctgataa tcggttatag catccatatg attggcaatt tggtcagcaa tatcatctaa
    10681 aatctgagat ccttctttcc aatatgcctt ataaccatta taagcttgag gattatgact
    10741 agcagtaacc atcacacccg aaacacatcc tagagcacga atagcatatg agcacattgg
    10801 tgttgggcga atccctttat aaatataaga cttaatacca tttgctgcca taatggaaca
    10861 agttaattct gcaaattcct tagattgata acggacatca taactaactg caattccacg
    10921 tgcaatagct tcagggccat gatcaataat cgtattagct aatgcttgtg ctgctttccc
    10981 caccatataa gtattcatac gattggttcc tgctccaagt ttacctctca atcccgccgt
    11041 tccaaattct aatgttttgt aaaaacgatc ctgaatttca gattcatcgc ctttaatagc
    11101 ttctaaatct gacttaatat ctttaccgag tgagtcgttt tctaaccact cttgataaat
    11161 ctctttataa ttcatatgag acatcctatt ctaagaccaa aatggtaaaa atcagctcca
    11221 tgataagaga ttttagttct tcaggagcta actgaatagt atcaaattaa ccttataaag
    11281 tcaacttatt ttataagtta actgcaccta atatatttaa gacatctaaa tttttttcat
    11341 catcctatca ctcttaatct acattatata tagacttacc aacttccaaa caatacagta
    11401 acaatttact cataacaatg gaataacaaa agtctaaaac aagttgtttt agacaatgaa
    11461 attataatac tttacttaag aaatctcttg tgcggatttc ttttgtctgc tcaaatactt
    11521 ccttaggggt cccttgctca acaataattc ccgcatccat aaaaatgaca cgatccgcta
    11581 cttcacgtgc aaaacccatt tcatgagtga caataaccat cgtcatacca gatttagcta
    11641 aatcttgcat aacagtcaag acttcaccta ccatttcagg atcaagagct gaagtaggtt
    11701 catcaaaaag aaggacatca ggattcattg caagacctct tgcaatagca attcgttgtt
    11761 gttgtcctcc agataagcta gctggataag cattagcctt ctctttgagt ccaacttttt
    11821 caagtagctc gtatgctttt gtctgagcat caagcttaga aagtcccttt gtcttaatag
    11881 gtgataaagt aatattttct agtacagtca tattgggaaa tagattgaac tgttgaaaaa
    11941 ccatgcccat tttttcgcgc attttaaaaa tatcattctt tttgtctgtt atatcaatcc
    12001 cttcaaaagt cactgttccc tttgttggta cttccaagag attcattgtt cttaaaaatg
    12061 ttgacttacc agagccagaa gggccaataa taaccaccac ttctccttga tgaatatcca
    12121 agtcaatgcc ttttaaaacc tcatttttcc caaaactttt atgaagccct tgtattgaaa
    12181 taattggctc aaccatctat ttttacccct ttcccaagat atttctccat ttgttttaac
    12241 aaagctgaga gaatcgttgt caacattaaa tagtaaaatg ctgcaaataa taacggtgca
    12301 actggtgagt aagtagccgt tacaacggat tgtgctccgt tccataattc catgacacca
    12361 atagtttgaa ggagggcact atccttaata attgtaataa attcattccc tagagcaggt
    12421 aaaatatttt taaaagcttg gggtaagata acatagcgaa gtgtattttt aggtcgaatc
    12481 cccaacgagt aagctgcttc tatttgtcca gatggtacag cctcaatccc tgcacgtaca
    12541 atttccgaaa tataggcacc actatttaag gaaatgataa tgataccagg taaaagtcgt
    12601 gtaaaatcta aatctaaaac accaaagcta atcgttggta aattgttaaa atgcatccat
    12661 gcgaaagcaa tcataatttg aactaccatc ggtgtcccac gaaataccca tacatagaaa
    12721 ttagctaata ttgtgagaaa atgtaaatta gtacgcttta ctaaagcaat taacacgcct
    12781 ataatagttc caaaaaaaac aacacatgtt gaaatcataa tggttaccat cacaccataa
    12841 ttaaaatagg accaatattg tggtaaaaat gaaaaattca ttgacatctc ctaaaaaagt
    12901 aattgccaat agtctatcac acaaagaata aaaatacaag tttaaataaa tatttttgtt
    12961 tttattctat tttactgtat atttagatat aaaaaaaaga ccttcaaggt cttttataat
    13021 tatcagtata ataaatcgta aatttccatt gcgataaggt caatgctgtc aaatgaatat
    13081 gattcacgcg cttctacatc acgtaatgtg aaaacaggac cgttttcagg gtcattacta
    13141 aaagatactt ctgctacaac aacaccttca cgttcaaaat tacgttttaa attaccacca
    13201 tcagtcgcca ttaattcgag gcgattgatg attctcacta aatgtgattc catcttactt
    13261 actcctatat attttctagt cctttctatt ttaacacaaa agtgaccaaa gcaacatgaa
    13321 aagcaacatt tttactaact ttttaacgat tttatcgtct aaaatccgca aataacgatt
    13381 tttctcagta gtcatctatt tttcaattta gcactctaat atattcagtg ctaaaaacag
    13441 aacttttttc ttgatttcta ggctttaggt gctataataa aagcataaag tttgactata
    13501 tttgactata tacacatcaa tagttaaatg tcataagaaa ggggtagcct atgctctgtc
    13561 aaaactgcaa actaaacgaa tcaactattc atttgtatac aaatgttaat ggaaaacaaa
    13621 agcaagttga tctttgtcaa aattgctacc aaattattaa aactgaccct aataatccac
    13681 ttttttcagg tttaaatcat gtttcacatg cgcctggtgg catcaatcct ttctttgatg
    13741 atttctttgg agatttaaat aatttcagag cctttaatgg ccaagattta ccaaacactc
    13801 ctcctacaca atcaggagga aaccgaggtg gaggaaacgg taatggacga aataataatc
    13861 gcaaccaaac tgcaactcct cctcaagcca aagggattct agaagaattt ggtatcaatg
    13921 tcactgaaat agcccgccat ggtgatattg accctgttat tggacgtgac tcagaaatta
    13981 ttcgtgttat cgaaatcctt aatcgtcgta caaaaaataa tcctgtactt attggtgaac
    14041 ccggcgtagg gaaaactgct gttgttgaag gacttgctca gaaaattgtt gatggtaatg
    14101 tgcctcataa acttcaaggc aaacaagtta ttcgtttgga tgttgtgagt ctagtacaag
    14161 gaacgggcat tcgtggtcaa tttgaagaac gcatgcaaaa gttgatggaa gaaattcgtc
    14221 aacgtcaaga tgttattcta ttcatagatg aaattcacga aatagttgga gctggtacag
    14281 ctggcgaagg tagtatggat gcaggtaata ttttaaaacc tgcccttgca cgtggagaat
    14341 tacaactagt aggtgctact actctcaatg aatatcgtat tatcgaaaag gacgctgcct
    14401 tagaacgccg tatgcaacct gtaaaagtcg atgagccttc tgttgaagaa accattacta
    14461 ttttgaaagg tatccaaaaa aaatacgaag attatcatca cgtaaaatat aataatgatg
    14521 ccatagaagc agctgcagta ctatctaatc gttatatcca agaccgcttt ttacctgata
    14581 aagcaataga cttattagat gaagctggtt ctaaaatgaa cctaacacta aattttgttg
    14641 atccaaaaga aattgatcaa cgtctcattg aagcagaaaa tttaaaagtg caagcgactc
    14701 gtgaagaaga ttacgaacgt gcagcttact tccgtgacca gattgcaaaa tataaagaaa
    14761 tgcaacaaca aaaggtcgac gatcaagata cacctattat taccgaaaaa acaattgagc
    14821 acatcattga agaaaaaacg aatatccctg ttggtgattt aaaagaaaaa gaacaatctc
    14881 aattaattaa tctcgcagat gacttgaaac aacatgtgat cggccaggat gacgctgtca
    14941 ttaagattgc aaaagctatt cgtcgtaatc gtgttggtct tggtagccca aaccgtccta
    15001 ttggttcctt tttatttgta ggaccaaccg gtgttggtaa aactgaactt tctaaacaac
    15061 tagcaattga gctctttggt tcagctgata gtatgattcg ttttgatatg tcagagtaca
    15121 tggaaaagca tgctgttgct aaattagtcg gagcgcctcc aggatacgtg ggatacgagg
    15181 aagctggaca attaactgaa aaggttcgtc gaaatcctta ctcgctcatc cttctagatg
    15241 aaattgaaaa agctcatcct gatgtcatgc atatgttctt gcaggtcctt gatgacggtc
    15301 gattaacaga tggacaagga agaactgtta gttttaaaga taccattatc atcatgacct
    15361 caaatgctgg ttctggtaaa actgaagcaa gcgttggctt tggtgcctca cgagaaggta
    15421 ggacgaattc cgtactaggt caactaggta acttcttcag ccctgaattt atgaaccgct
    15481 ttgacggtat tattgaattc aaggctttag ataaagagaa tctcctcaat atcgttgata
    15541 ttatgttatc tgacgttaat gcacgtctcg ccatcaatgg tattcatcta gatgtcactg
    15601 ataaagtgaa agaaaaattg gttgatttag gttacgatcc aaaaatggga gcacgtccat
    15661 tacgtcgtac cattcaagaa catattgaag atgctatcac agattactat cttgagaatc
    15721 caagcgaaaa agaacttcgt gctattatga ctagcaatgg aaatatcata ataaaatctt
    15781 ctaaaaaaac tgaggaaagt gcaaaaggtt gagtaagaaa ctcaaccttt tttcacactc
    15841 ttctcaaaaa ataatagcct ctataaagct ttttctctcc tatctcgtct ttaaatttta
    15901 atacaattat taaggaaata ttccaaaaat tttagtataa taaataaaaa ataattaaag
    15961 cttttaaaat cataaaacac aatcaggaga gagaactatg actaatccta ctttcggtga
    16021 aaaaatagat aatgtagact atagatcacg atttggtgtc tatgctatta ttccaaaccc
    16081 aactcatgat aaaattattt tagtacaggc acctaatggt gcttggtttc ttccaggtgg
    16141 ggaaattgaa gaaaatgaaa atcacctaga agctttaacc cgtgaattaa ttgaagaatt
    16201 aggttattct gcaacaattg gccattacta tggtcaagct gatgaatatt tttactctag
    16261 acaccgtgat acttactact ataatcccgc ctatatttat gaagttactg cctatcataa
    16321 ggagcaagcg ccactagaag atttcaacca tctagcctgg tttcctatcc aagaagctaa
    16381 agaaaagcta aaacgaggaa gccatagatg gggtgtccaa gcttgggaaa aaaatcacca
    16441 ttctaggaaa taattcatac ctttcttcct aaatagtgtt ataataaacc taataagaat
    16501 cagggggaaa atagttatga gactaattaa tacaaccagt agtcatcctg aactagttcg
    16561 aaatcaactc caaaatacag atgcaaaatt agtggaagtc tattcagctg gcaacaccga
    16621 tgtcgttttt acaaaagcac ctaaacatta tgaattatta atttcaaaca aatatcgtgc
    16681 tatcaaagat gaagaattag aagctattcg tgagtttttc ttaaagcgta aaatagatca
    16741 atctattatt attcaagagc agatgaaatc actccatact gccaagttaa ttgaaatttc
    16801 atatccaaca acagcataat taaaagaagc ctaatcaagg cttctttttt gatgactact
    16861 aaacaaatgt cttaccaacc tcttccatta aaactggcaa gatatttgtc aaaatatagc
    16921 gttaaataat ttttattaag cttcaaaacc ttgagctact gcttcaggat aatattgctc
    16981 aataattgct gcactagcat cacaaacagc tacaccatac gaacgcatct ttgtggttgg
    17041 atcaatacgg cgtgagcgtt cgcagacttc accttcagcg tgttcaacag taaatgcaac
    17101 accttcaaat gatactgagt cagctggttt gtctgcttca tcagcaattg ttaattgtga
    17161 gacaatcatc aagagtgcaa tatcgctatt taacgctgtt aataacgttt tcacttcttg
    17221 actagcataa attgttaaat gtgcttctag agatttacca ataactttag cgttacgtgc
    17281 ttcttcgaga gctttttgag cttgtgttcg taacgtcatg aatgctgacc actcttcaag
    17341 aatttcttct tgaccagaga atgtctgtgc aacaggcatt tctgctaatt gaacaaactc
    17401 ctcctcctca tgttcaaggt atgaccaaat ttcttcagca gtatgcggta gaatcggagt
    17461 caaaagtttt gtaagtttaa ctaaaatatc atagaaaaca gtttgcatac gacgtcgttc
    17521 tggactgtta gccgcttcaa tgtaaaccac atcttttgca aaatcaaggt aaaatgctga
    17581 tagatcaact gttacgaagt taacgacagc tttataaatt gccataaaat cataagcagc
    17641 ataagctttg ttgattgtat ccacaacttg attaaattta atagtcatat aacgatcaac
    17701 ggcacctaaa ttttcatagg ctactgcatc ttgtttagga ttaaaatcgg aagtatttgc
    17761 aatcaagaaa cgcaatgtat ttctgatctt acgataagtt tctgatgttt gttttaagat
    17821 atccattgaa atacgaacat cattacttga gtcaacagaa gttacccaca gacgcaagat
    17881 ttccgcacca aattgttttt caacatcact cggaagaatt gtatttccta gagatttaga
    17941 cattttctca cctttaccat caagtacaaa accttgagat aagactgctt tgtatggagc
    18001 gtgaccatta acagcaactg atgtaattag tgatgagttg aaccaaccac ggtattgatc
    18061 agaaccttca agataaagat ctgctggata ggaaaggttt tcacgagcat tcattactcc
    18121 attccatgat gaaccagaat caaaccaaac atccatgatg tctgtttctt tctcaaagag
    18181 accattaggt gaacctggat gagtataacc tgctggtaga aggtccttag catcacgttg
    18241 ccaccaaacg attgagccgt attcagcaaa taaatctgca acgtgatcag tcacttcttt
    18301 tgtcatgata gctgtaccat cttctgcata aaaaattgga agtggaacac cccaagcacg
    18361 ttgacgtgag attacccagt cgccacggtc acggatcatg ttataaagac gttttttacc
    18421 ccactcaggt tggaaatttg tcttttcaat ttcatcgagg atttcttgac ggaatttcga
    18481 aacagaggca aaccattgtg gaaccgcacg ccaaatgatt ggctttttcg tacgccagtc
    18541 aaatggatat gagtggttga taacttcgct ggctaaaaga agatctccca atttttcttt
    18601 tacaagtggt gtcactttgt catagaattg accttcaaaa tcaggaccag cattttccat
    18661 catcaaacca cgactatcta cggtaactac aacatcaagt ccattagcaa taccaacgtt
    18721 atagtcgtct tcaccaaaac caggagccgt gtggacaata cctgtaccag aatctgttgt
    18781 aacatggtct ccaaggataa ccaactcttc tacttctgta tcccatggat gttctgtaac
    18841 aatgtgatta agttctttac cagtgtgatg agtcacaatt tcaaagtttt cccagccaaa
    18901 cttagcagcg agactatcta caagaacctc tgcaagaagg tatttacgct cactacctac
    18961 tggtgcaact acaacatact ccatatctgg tcctactgtt aaaccgcgtg aagctgttac
    19021 agtaaatggt gttgtcgtcc aaacgacgat ataggtatct gtatcaagaa ttcccttacc
    19081 atctttaact ttattggcat agtagagtga tgtcgaatca atatcatgat attcgatttc
    19141 agcctcagca agggcagact ctgatgacca tgaccaatac actggtttag caccacgata
    19201 gatataccct ttatctgcca tagcaccgaa aacacgtact tgatctgctt cataatctgg
    19261 tgtcaatgta atatagggat tttcccaatc ggcagaaacg cccagacgtt taaaatcatc
    19321 acgttgtttg tcaacttggc tgagagcata atcacgacac atttcaaggt attcagccaa
    19381 gtccatttct ttacgtttaa cccctttttt agctaatact tgctcaattg ggagtccatg
    19441 agtatcccaa ccaggcacat aaggagctcg aaaacccgac attgattttg aacgtacaat
    19501 aatgtcttta gaaattttat ttagtgcatg tcctacatga atattcccat tagcataggg
    19561 aggtccgtca tgaaggtgaa aggctggttt tccttcattc aatgcttgac gttttttata
    19621 aatgtcagct tgatcccatg cttcttgcca ttgaggttcc ttatttggaa gccctgcacg
    19681 cattggaaaa gctgtttgtc ctaaattcaa ggtttctttt aatttcatac taactcctta
    19741 tctcatagta atagttatca caatttttaa aattgctaaa taaaaaagct ttcatcacat
    19801 aaaggacgaa agctcgtggt accaccttaa ttcagagcag aaaatagcta tttcttactc
    19861 cctcaacaac ccgtaaggtg ggttaagcct tctaccttac ttaaataata gttcaggtag
    19921 aatgattgaa aatgataatt tcaatattag ggattgtagg gctctcacca tctcctactc
    19981 gctgataata taatattaaa accttgtttt tcttaatctt caatatttaa tttaaaagtt
    20041 tgagtttcac ctaaattgat cggttcttcc atttggaaat tattagtatc taataaacct
    20101 gactcttcaa gttgtttgtt actttcttca acacgacgct gtaattcttc catttcatct
    20161 ggtgagaatt gacgagtagc atcaaatgac tcagtgtcat ccaccactgg taaagcatca
    20221 tcttcatcaa gaactttttc aacaacttct ttgaaagaag catcagaatt ttgaagataa
    20281 atagctgttg gtttcaacag ttcttcccaa gcagatgagt tagctaattt taactgacct
    20341 tctaattcag aaagtaagcg ttggtggaaa acacgtgatt gacgtttcaa atcttctgtt
    20401 tctatagcga cacgcttagc atcatctgtc gcatcacgaa ggatttgatt tgcttttaat
    20461 ttagcctcat caattaaatg ttgagcatca aatgtagctt tccccattag gttagatgct
    20521 tcatcctgtg ctgaaatttt cacacgttca gcagtttctt gagctaaaat aactgattga
    20581 cttaacgatt ccttcatttc gttgaagtaa gcgattttct cttctaaatc tttaatgtat
    20641 tgctcttgct cacgattacg tctaatcaag tcttcgtaat cgtcaacaac aatctctaaa
    20701 aattcattaa cttcttcttc gctataaccg cgaaattttg atgaaaatgt tttatcttta
    20761 atttcaagtg ctgtaagtgg catgttttct ccttagttat gtatcatttt gtctacttct
    20821 aacttatatt tttgattttt agttaaccct aggttatggt tcaacgtaaa tcgaccatat
    20881 cctctaacac taatcaaatc cccctctact aattgttcag aaactcgatt gacgattgca
    20941 taattgacct tgactttatc tgcctcaatc aatttcgtcg actgtgttcg tgaaatcttt
    21001 aaaatcgttg ctaaaatttt atcaagacgt aaactggaag caataacagt tagtttttga
    21061 atatcttgtt tgggagttag aagtttggac aagggaactt cagctaattg tacactagca
    21121 gttccaattt ttgtaactga atgaacaagg tgatttgtca tttgactatc gaccaaaacc
    21181 tgagcacagc cttcttcaac taggatatcc cctaaaattg atcgttttac acctaagtaa
    21241 ttcagtaaag ttcccataat tttagcatgg gtaaggtgat taaatttagc attatacttt
    21301 atttcaatta ggctaaggtt aaaatcaacc atatctaact gatagtattc cggtgctatt
    21361 attactttaa catattctgt ttgaaaatac tgaccagaag tgtaataatg actacctcgc
    21421 ctctctagaa cactttcgag tattttaaat tccctaggat ttagaaattc agtggtaata
    21481 aacgaatagg tgttttcgac atattgagct aaatggtcta ttttattgat aaacgcatac
    21541 tcttctggtc gaaaatgctg ataaatatca tctaacgtca tcttatgtaa aaagcaagat
    21601 taaaaatcta ctcaacatat ttagtaaaaa gaatgctact attactgtaa aatctagacc
    21661 taaaaattgt agattaaatc ttctgagtgg gcgaataatc ggatcaacaa tctgtaccac
    21721 tagtctacca atccatgaat cgtatgctcc cggaaaccaa gataacaagg cataggctag
    21781 taagatataa gaatagaccc taattgtttt taataatatt aatagtacta atatcatatt
    21841 tatcgtctct tcatatcaaa atcaaaactc gtctcttgac cagtctttgg aatattcatc
    21901 tcctcaatat cgaccataac attggctggt gttaatagaa acattgaact cccaaccttt
    21961 tgtaatgaac catataatac tctacttgca ccatcaatat aatccaagca acgtcgtgct
    22021 tgcgcatcaa gcatatattg aaaatcaatc aagacacatt cattaacgat aagtaaatca
    22081 acaatttctt gtgcatcctc atacttacgg ggatacttca gagcaattgt tacttgttct
    22141 ctctgttgtc gaactgagtc tttattttga atcctaggag tagcttgctg ataagcattg
    22201 tctctctgta ccctttgtct ctcctgacga ttaggattat aggtagaagt tctcggacga
    22261 ctaccaatca tctcttcctc tgcggagtta gtcatatgac tacgctgaga agcttcctgt
    22321 gctgtagctg ctctggaatc tctttgcact gaagttctct cttgtacttc gtgtacttca
    22381 ttttcactta catcgtcagt gtcaaaataa gaaataattt tgtcaaatct atcttttaat
    22441 gccatattcc cctccattat ttaaagaatg aagttcctat ccttacaaat gttgatccat
    22501 tttgaatagc aatatcataa tctctactca tgcccatgct taattctgta aacggcattc
    22561 gttctatgtt cctagcttga agccttttcc taagactctc agtctcttca aaaattgatg
    22621 atatttcctc tttagttgct tttaagggag ccatagtcat gatgccgata agttcaattt
    22681 tatcgtaccg agaaattaag ttcagtgcat catctatttg ctcaatagta aaaccatgct
    22741 tactgtcttc tcgtgatata ttaacttgta gaaaacattt aactggtttt tgagcacgtt
    22801 tttgaatctc agctgctaac ttaacagaat ctaaagcatg aaaataatca acataattaa
    22861 tgacatcttt aacttttcga cgttgtaaac taccaatgag atgccatgta agcttttcat
    22921 cttttaatgc ttgatatttt tcaagaaatt tatcaacacg attttcaccg atatgattaa
    22981 cacctgttct aataagcgct tctgttgttt gacagttaac atattttgtt acagctacga
    23041 catgaactga ttcaggagcg cgacctgctt ttagtgccaa tttactaaca ttgtcaaaaa
    23101 tcgctgtttt attttcttgt aaattcatca ttttaattaa cggtttttaa agaatggagg
    23161 tgtttcaagt tcatcatcca tatcatcatt ttctgaaaac gttgacatag ataacttgct
    23221 atcaagttca ccttctgtcg gacgtgaaat attatcacga cgaaggtccc aattaccaaa
    23281 agcagaactt tgttgctgat tttgtgtatg aggctgattt tgttgagttg gcatttcgcg
    23341 agactctgtc atatcaaagt tgccacgacg atcaaagttt gaatttgaag tactttgacg
    23401 ttctgaaggt gcttgattag ttggagctga tgttgtaaaa ccagatactt gattagtttt
    23461 atctttacgc acacctgttg caacaactgt tacgcggatt tcgtctttca tatccatgtc
    23521 aattgaagtg cctaaccaaa tattgacacc tttacctgct gcttggctaa caatctctga
    23581 tgcttcctct gcttcagtaa gtgtcatatc cataccgccg gtaacattga caataacgtc
    23641 ttctgctcca tcaatcgttg tttcaagaag tggtgaataa attgcttttc gagcagcttc
    23701 agtaatacgt tcctcaccag aaccgatacc aattcccatc agtgcattac ctttatttgc
    23761 cataactgtt ttaacatctg caaagtcaag gttaattaaa cctgggttag taatcaaatc
    23821 tgtaatacct tgaacacctt gacgtaaaac attatctgct tcactaagag cttcaagcaa
    23881 tggtgtcttc ttatctacaa tttcaagaag attattattt gaaatgataa gcagtgtatc
    23941 aacttgttct cttaattctt gaataccttc gatagcgaag ttagaacgtt tattaccctc
    24001 aaagccaaaa ggacgtgtga taaccgctac tgtaagtgct cctaaactct ttgcaatacg
    24061 tgcaataact ggagccgcac ctgtaccaga tccgcccccc atacctgctg tgataaatac
    24121 catatcagcg ccagtaagtg cttctgttaa aacttcttca ctttcttcag ctgctttacg
    24181 accaacttca ggttgtcctc ctgcaccaag tccgcgagtt aattttggac caagttgaat
    24241 gacagtttca gcttttgagc tacttaaggc ttgaatatca gtatttgctg caataaactc
    24301 aacgccagcg acaccttcgt caatcatgcg gttaatagcg ttaccgccac cgcctccaac
    24361 accaataact ttaataactg caccttgtac tgatgctgta tcaaatgaaa ataccatttt
    24421 tattcctcac ttattgtttt agtcaaacat acttccaaaa attcctctga tgcgctcacc
    24481 aatattagct ttaggctcag taggctcttg agcgttttct cttgctcgga ttggttctaa
    24541 attagaatct tccatagaag aggttaacgg ctcagaataa ggcattgtcg acattgtatt
    24601 tgttttttct ttataatcaa agtcgactgg tttatgtctt agcatttcat cgccagttac
    24661 agcatgttgt gcgataatgt caacttctga catcattcct acataatcaa cgatactaat
    24721 aacattagcg aacattggat tgcgaatacc tacttggttt ggaacgtgca atttaactct
    24781 agttccaaaa atttgttgag caacctcgac aactcctggc ataattgcac cacctccaac
    24841 aagaataata ccaccaggta aatcaagtaa acgacctcta cccaagtctt gtttgacatg
    24901 ttctaaaatt tgacgaatac gtcctgaaat aatttgcgaa agatagcttt ctgtaatttc
    24961 aacaggttcc tcgttaccaa caacattaac ttgaactgta tcagaagtac ttgcttcttc
    25021 aacattagct tgtccaaagt tgaatttcaa tgcttcggcg atttctactg ttgtacgtaa
    25081 cactttagaa atatctttgg tgacataatc gctaccttca gaataaatat tagtatattg
    25141 caattcttga ttacgcatag aagcgactgt tgtttgtccg cctcccatat caatcacagt
    25201 tgcaccaaac tcacgttcac cttcgtttag aactgatttt gctaaggcta gaggggcaat
    25261 cacaacatgc tctactttta tacctgctcg ttcaacagtt ttacgaagat tatgaaggat
    25321 agttgttggt ccagtataga taagaccacg catctcaagg cgaatcccca tcatccctct
    25381 agggtctcta atcccttgga aaccatctac aatgaattca agcggaatca atgaaataac
    25441 ttctctttca ggagtaatgc ttttagtcaa tgctgattta acaacacttt cgacatcttc
    25501 atccttaatt tcttttgatt cattgggaac aggaatcatt ccttgagttg gttcaatttg
    25561 gagaagattt gctggtaatc caacattgat tttgtcaatg gtgatgcctg ctttctcctc
    25621 agcttgtttt accgcctctt tgattgcagt tgctgctgcc tcaatatcaa taattatacc
    25681 atcttttaca ccggaactag ggacgttgct aaccccaatt acattcattt catttgcaat
    25741 aaattctgca actaaaactt taatcgagct tgttcctata tccaaaccag taaaaaagcc
    25801 atttctagcc attcactcga cctcgctatc tttccattct tttctattct acatctgcca
    25861 aaaacagaat tattcagaaa atattatacc ataaaaaaag cagaattgtt accaaattac
    25921 gtttttttat taagcaacat caaattgaaa tatttctaac ttatttaaac cattcgcaac
    25981 ttataacaaa cttaaatttt tttcaagaaa tcaattttta ctagcaatct atgactgact
    26041 tgatatgtat aaaaaagact gataaatcca gtcttttttt atcgctaatt aacattttga
    26101 gggttgggtg cctgctctgt tgctatctgt tgtccttgtt gattagtatt tgagttattt
    26161 gtttgtcctt gactattttc cgcaacctga ccattttgtg tttgtgtttt atcagttgat
    26221 ttattttttg tatcttccgc tttcacaggg gttgattcaa tagtacttgt tgttgtgtaa
    26281 actcccactt ccatatcaac aatagaaggt tccttaaggt tcttcttaat ttgtttgtaa
    26341 aaaggaagtc tttctttaaa tttagataat ggtattctaa tactatttcc atcatgcata
    26401 tctaacagca ggaggtcagg tgtcgtttta gaatcagcta aacttatcac ctgaatctca
    26461 cttattaaat cagggtctaa agcctttaaa tctttaatta atagcttaat actatcttcc
    26521 ttatcaaggt taattgttaa gaagtgcttt ggtagctctg aactatttac aggatcagcc
    26581 ttttttccag tttccaagac aggctgatat ccttgctttg tatgtgcata tgcaataatc
    26641 ttattttctt gaacttgaat atgaaactta ttgggaaatt gataagtcat ctgagctgtt
    26701 tttacccata catcttctgc agctaaacgt tgttcaatag ctttatgttt aaaaattaaa
    26761 gaaaagaaat aatcgttttt ttgaatattc gttttttcta tcaaaatatc atcaggtgta
    26821 tgctgatttc cactaactgt tattgttttt tgcttactaa aaggagttag taggaaaacg
    26881 gaaactaaaa tgactaggaa tgctactaca aatataggtg cagttctaat gcgattagtt
    26941 ttggctaagc tgttgactat tttttctttt ttctgtttct tttcaatctt aggttttgaa
    27001 atctttggaa aatgaagctt cttaatttta gtagtatttt gaggttcttc aggagaagaa
    27061 atatttaatt tacttctttt atctaagcgt aatttttcgt taatacgttt ttgttcttct
    27121 tcatcttctt tgcgtttttt taaaaattca aggttacgct tttgccattc cgttaagaca
    27181 acttcttctt tttctggggt atctgatttc ttcttaggca tgttttatcc ttttgttact
    27241 tttgccatat catcaattaa taattgataa aattcatctt gagattttat ttcagatgat
    27301 tgtgacatgt ttttttcgta actctcactg ttagaaatta ataaatttat ctgtttctca
    27361 agtgtattta tgtttaattc agattctggt aactgcaatg cgtagccctt ttcttcaaaa
    27421 taagcagcat tctctaattg gtcacctcgg ctcgcttcac gtccaagagg gataataaga
    27481 tgaagttttt tcatagctac taattcaaaa atagtattag agccacctcg tgtaacaacc
    27541 acatctgcta aattcattaa aggctgataa aggtctgtaa cataatccac gcgataaagg
    27601 ttcttcttca atcgattgag agaagaatcg cctgaaatat tgataacatt atatttttct
    27661 tccagctcag gtgtttgagt aataaaatca ttgaatactt ttgcacctgc tgatcctcca
    27721 ataaatagta aggtctttag gtttggatcg aaggcttctt ttatactagt taagtcagta
    27781 ttttcaaacg actttttaca atccatgact tttgttacag caccaatatg cttagttttg
    27841 attaaatctt tagattgttc aaaagtcgta tacataatag tcgcaaattt ataagcaatc
    27901 ttatttgcta accccattga taaatctgat tcatgaacaa aaacaggaac ttttaatagg
    27961 cgtgcagcta ctacaggagg tactgaaaca aatccccctt ttgaaaataa ggcttggggt
    28021 ctaagtttag caataatagc aatcgattga agaacgccaa cccctacttt aaagacatct
    28081 aacatatttt gccatgaaaa atagcgacgt agtttcccag tagctataga gtgaaatgtg
    28141 atatcaagtc cagactgatt aatttgttca tgttctatgc catttttatc accaatataa
    28201 tgtacttccc aaccatcttt gataaatttg gggattaata ttaggtttag tgttacatga
    28261 cctaccgtac caccacctgt aaaaacaatt tttttcccca tagttactct cctctaagac
    28321 tttcgaaagt atcaatgaat tcatcaccac ggacttcgaa attcttatac atgtcccatg
    28381 atgcatttgc aggacttagc aagataacat cgccctgttg tgccacctca taagctttat
    28441 gtaccgcatc tctaacatct aaagcatcgc tataagttac tcctgctttt tgtgcagcac
    28501 gttttactcg agatgccgat tcccctaaaa caaccatata tttaagtcca gtgatatctg
    28561 gtatcaattc atcaaactca ttaccgcgat caagacctcc tgcaattagg ataactttag
    28621 tattatcaaa gccagataat gctttttgag ttgccaatat attagttgac ttgctgtcgt
    28681 tatagaaact aataccatga accttaccga gtgattgcaa gcggtgttta acacctccaa
    28741 aattgcttaa agtttctcta ataacttgat tactgatacc agctagttta gcaaccgcaa
    28801 tagttgctag agcattctct acgttatggc ttcctgggac accaatgtca tctactaaca
    28861 taatattctc ccctttatag aaaagttgct tgtcttgtac gtaagcacca tcaacttttt
    28921 ccgtagtaga gaaaggaacg attgttgctt tagtagtttt agctaactct ttagaaatac
    28981 cttgattaaa attaagtacc aaaaaatcag atgaagacat ttgattttgg atattccatt
    29041 ttgcagcaac ataatcttca aaagacccat gataatctaa atgagttggc attaaattag
    29101 taattactgc aatatgagga cgaaattcct taactcccat tagctgaaaa cttgataatt
    29161 ccataactag aatatcttta tcatccgcag cctgaacaac ttcactagca ggaaagccga
    29221 tattcccagc taacaaacct ctctgacctc cagcatttaa gacttctgca atcatcgttg
    29281 tcgttgtcgt tttcccgtta gagcctgtaa tacctattag ctgagattct gaaactaagt
    29341 atgctaattc cacttcagtc aaaacaggga tttgtttttc taatgctttt ttgaccatag
    29401 gattgttata aggtattcct ggatttttaa tcatgtaaca aaaatcctca tctaacaatt
    29461 ctaaaggatg actaccacaa accactttaa taccctcttc caacaaagac tgtgctgttg
    29521 gattttcatc aaatggtttg ccatcattaa ctgtcactat tgctcctaac ttagctaaca
    29581 aacgtgcagc ggcttctcca gatcgtgcta aaccaaggac taaaactttt ttattttcaa
    29641 atgttgttat tgttttcatt actcgtccca ttctatcatt attatcccta ttattttacc
    29701 tttaatcagt ggctttttca aggtaaagga tatgtctcta atttctcaaa acaaaagaac
    29761 ttaggagaca aaatcctaag ttcataagct atttcttctt ttctgacaaa ttcaatattt
    29821 ctttaaatgc tgtatatcct agaggaatca ttataagccc aatatacaat tcacctggca
    29881 attgaaggat tttaataaaa ctcataactg ccaagacaag taataaggct accaaaccta
    29941 ctaaaccaat agcgttcaaa gtattacgta ctgtttttgg tgccataaaa aagtaatata
    30001 aggcaatcaa aagggcaaca attaagtaaa acatagaact cctttcttat taatcataaa
    30061 taagaaatac tttggatatt attcagctga ttttttcttt ttattagctt tgtcacgtgc
    30121 tgctttattt aaaatttgtt tacgaagacg aatagactca ggagttactt ccatgtactc
    30181 atcatctgct aaaaattcaa gtgactcttc taaagtaagg atacgaggcg tcttgatgac
    30241 tgcagtttga tctttagttg ctgaacggac atttgtcatt tgtttagcag ttgtaatatt
    30301 gactccgagg tcattatcac gagaattctc accaacaatc attccttcat aaacttctgt
    30361 acctggattt acaaagatag ttccacgctc ttcaatacgc ataattgaat atgtagttgc
    30421 tttaccattt tcaatagaaa ccaaggcacc acgatgacgg ccaccaattt ctccttgaac
    30481 aaccggtaga tactggtcaa aagtatgatt catgatacca tatccacgtg tcattgaaag
    30541 aaactctgtt gaataaccaa tcaaaccacg tgcaggaatc aagaaaatca aacgcgtttg
    30601 accattacca accatctgca tatcaagcat atcaccttta cgctctgaca aactttggat
    30661 aatagcaccc tgatattctt ctggagtatc aatttgaaca cgctcaaacg gctcgcattg
    30721 aacaccatca atttctttga tgataacttc tggacgtgat acttgaagtt catatccctc
    30781 acgacgcatt gtttcaataa ggatagataa atgtaattct ccacgccctg aaaccgtcca
    30841 tttatctggc gaatctgttg ggtcaacacg caaagaaacg tctgtttgta attctgctaa
    30901 aagacgttct tcaaccttac gtgacgtaat ccatttacct tcgcgacctg caaatggtga
    30961 attattcacc aagaaagtca tttgaagtgt tggctcgtca atacgtaaaa ctggaagtgg
    31021 ttcaatagca tctgtcggag ttactgtctc accaacgaag atatcttcca taccagaaac
    31081 agcgattaaa tcacccgctt tagcctcttg gatttcttta cgttcaagtc caaagaaacc
    31141 aaaaagtttt gttacgcgga agtttttagt tgtaccatca agttttgaaa gagtaacttg
    31201 atctccaact ttgacagtcc cgcggaaaac acgtccaata ccaatacgtc ctacaaaatc
    31261 attgtagtca agaagagaaa cttggaattg aagtggttct tccgagttgt caactggggc
    31321 tggaatgtga tcaatgatag tatcaaaaat cggtgccatt gttttttctt gatctgaagg
    31381 atcatctgac attgaagatg ttccattgat agctgaagca taaacaacag ggaaatctag
    31441 ttgatcatca tcagcaccga gctcaataaa gagttcaaga acttcatcaa caacctctga
    31501 tggacgagct gacggcttat caattttatt tacaacaacg attggaatta agttttgttc
    31561 aagagctttc ttcaaaacaa aacgtgtttg tggcattgtt ccttcgtagg catcaacgac
    31621 taaaacaaca ccatcaacca ttttcataat acgctcaact tcaccaccaa agtccgcgtg
    31681 accaggtgtg tccataatat tgatacgaac atcgttgtat gctacggctg tattttttgc
    31741 aagaatggtg ataccacgtt ctttttcgat atcatttgaa tccattgcac gttcttcaag
    31801 ctctttacgc tcatcaagag tatgtgattg ttttaataat tcatcaacga gagttgtttt
    31861 accgtggtca acgtgggcaa tgatcgcaac gttacggata tctgttctta aatttgtcat
    31921 gttttcctct attttataat gtaattctgt taacttatga attataacac aatttttctt
    31981 aaaaaacaga atttacacaa gtgtaaatgt tttcatatca actgcctgtt tgtgataaga
    32041 aaacttttca tcatttcatt atttatgtca tttattttct acttttttca atttgattca
    32101 tttctacaaa aagaggagag agttattaat agtaactctc tccttagtat attacttaac
    32161 tctacccgtc cagtaattga aaccgtcctt taatacgtaa agttgattaa aaccttcttt
    32221 tctgagtaac aaaacaattc ttggaataga ttgaccacgg cttgcgtcat ataagaggac
    32281 aggtttatct ttgcgtaaag ccgataaggc aactttaaat tggcttgctg gaatgttacg
    32341 tgctccgaga atatgttttc tatgaaaagc gccagcttct cggatatcaa ttaactgtcc
    32401 tcgtgacatt tctttttgaa aagactcgtt atctaaaaat ttagctgccc tacgaaccct
    32461 ccaataattc caagatgccc aagcaacaaa agccagaaga ataacgataa ttaaaataac
    32521 agacatatcc atatcttatc tccttaactt gcttgttgat tggctaagct tgctgcacca
    32581 ataataccag catcattacc tagttcagca atcttaattt tagttgactt tttaacttgt
    32641 gggaaagcaa atgtgacaaa gtatttctca acgcgactac gtaaaaattc acctgctgct
    32701 gagacaccgc caccaataac cacagaatca gggtttaaaa tatttgaaat attagctgct
    32761 gccagtccaa ggtaacgtga tacacgttca acaacagaat tagcaaattt atccccatct
    32821 tctgctgcta taaaaatatc tttacttgta acagtatcac cgttgtcaat cgctgcttta
    32881 atggcagacg aaccttcata ctgttctgcg agttgacgtg ctactctaac aacacctgtc
    32941 gctgatgcaa ctgtctcaag acagcctttg ttaccacatg tgcacataaa tccattttct
    33001 ggatcaacaa tcatatgccc aatttctcca cctgctcctg caacaccatg gatgaggtta
    33061 ccatctgcga taacacctcc acctactcct gttccgaggg ttacgaaaac aacgtcggga
    33121 ttattggcac cagcacctac ccagcgttca ccaagtgctg caacattagc atcgttatca
    33181 ataaaaaatg gaattccaac ttctttttca ataactgaac ctacttcttg agtatcagcc
    33241 caatttagat taaaagcacc tgttactgtt ttactagttc tatcaacagc tcctggagac
    33301 cccataccga taccgagaaa gtcatctttt gttaatccat agaggctcaa acgatgtttg
    33361 agagattcaa cgatatcaga aacgatatgt cttccgtttt ctaaagtatt ggtctcaatt
    33421 gcccattttt cttgtacttc tccctcaagc gtcaagatac caaatttaat ggtcgttcct
    33481 ccgaggtcaa ttcccaataa tttcttactc attttttatt ttccttctcc aattctagtc
    33541 tgtgctctct tcttaaaatc agttctgctt ttaaataatc atctctactt atcagaccat
    33601 tatcgtagag acgttgcaat tcaatcttca taacttcgat atcataaaga cgtttaccaa
    33661 tataaacata aataccaaat tttttcaaaa gattctgcac atcgaataaa actttcatat
    33721 atgaatttta acagaaaacc attcaatttt caagaaaatc cgcaaccgct tgctaattgt
    33781 cattatgacg ttaggctgac tatctataat agaaatcata atctcttttc taccattttt
    33841 cgaataataa atataaacta taaaaaggat tttatgatta tgtctaccat ctattttatt
    33901 tctttatgca tgagttttat cttaagttat tacgatataa aataccaaga gtatcctatt
    33961 tttctatgga tattattcac catttcaacg atagttctca caccaattac aaaagtttct
    34021 attgttttgt gtttatttgg gattttagct gaagtggtgg atattaatat cgggagtgga
    34081 gatttccttt atttagccac aattggctta tctttacctc ttcatcaaat gctttttatt
    34141 attcaaatag gtgcttggtt aggaattatt tattgtttag ttatgagaaa aatgaaaaaa
    34201 acgatagctt ttctaccgtt tttaagtatc gcttatataa tcgtcaccag ttactctctt
    34261 ttattctaat cactttaaat tatcaggaaa gtgataaagt tgtgggtact ggtcgcactt
    34321 tggattttta ggatgacaaa ttgcacgacc aaaataaatc atggattgat gagcagctag
    34381 ccactcttct ggtggtaaaa cctccatgac tcgtttctca atttccagtg gagaagccga
    34441 ctgtttgcaa atctggtggt gtttacaaat ccgagtgaca tgagtatcta cggcaaaagc
    34501 cggaatacca aatccaacac tcatgaccac attagccgtt ttccttccaa cgccagacaa
    34561 actttctagc tcttgacgag tttgggggac ttttccatca aaatgctcta tcaattgttt
    34621 agcgcattga tttaagaatc gagctttatt acggtaaagc cctattttag aaatataggg
    34681 ttcaatctct ttgggatcag cttgtgccaa aactagagga tttggaaagc gttcaaaaag
    34741 ggcgggggtg actttattaa cagctgcatc agttgtttga gctgatagca ttacagctac
    34801 caaaagctca aagacattag taaagtctaa gctaggttta gcatctggaa ataacttaat
    34861 aatctcacgg ataatatatc gtgattttgc ttttgataac attttattcc ttatctttct
    34921 tttttatcat tatatcacga tatctttcaa aataaagaat atcagaactg aggtttgcaa
    34981 tccattaacc ctattttatt tttttagtaa aagctcatgc taattgtctt ttgagttgtt
    35041 ggtaatcaat ttttccagat ggcatcaaag gaattttaga tacaatatga taatgcttaa
    35101 aaccatactt cttttcaaaa tgttccgata accacttcaa acactcttgt tccatctgat
    35161 tttcaattaa aacaatacag caatgtgact cttgtccatg actttgactc tcttttgtaa
    35221 atgctactgc atccttgata taagggcatg tttttatttt ttcgattaat ctaggtaaat
    35281 agagcttaat gcccgattga ttgcaccaat caccaccacg gccaaataga tataatttgc
    35341 catctatcag ttcaccataa tcaccagcac aatattcact tgaaaggcca cagatactat
    35401 acggtgtttt tacaaaaatt cttccctctt taatcctaac agctacattt ggaacgattt
    35461 ctccaacata ctgtggctta tctcggatat cacgaccatc ataccatgac acataattga
    35521 gctcactagc accatagaag attttcactg acactttagg atgctttata tataaacctt
    35581 ccagtaatga gtcagagaca tattgcgaag aggtaataat agttttattg tctaacgcag
    35641 tctccttgct atattgttcc actaacttga gataactagg gagcagatat aaatgcgtta
    35701 caccagtttt ttcccataaa gtctgccagt attttacaga gtttttctgt gtgacaacaa
    35761 gcgttccccc cagtaataaa agggatagcg ctaaatttaa gttaccagta aatgaaaaat
    35821 caccttgaat aaacagtctg ctattggaag tgaccgaaaa ataagcattc tgaatgctaa
    35881 aaaaatcaga ccacgaagcg aagctcctcc acaatagttt agcatcagct gtagtcccag
    35941 aagaaagaac accaaaatca gctagttgag gagcatactg cacttctttt tctaatcgac
    36001 ttatttcatt atgagtaagg ttaggtttac aaataatagg aagagctttt tgacataact
    36061 ttgaaacaaa tcttactaat tggttaagca gagagtgtgt gctaatcaca tgtttcctat
    36121 tgtcttgtga cgccatatct tgtctgacta agttataaaa ttctccataa ctgacctcta
    36181 aatcaccatc aaataacttt ttatcgctat tggttttaac tattgttttc aatgattcaa
    36241 gcattcttaa ctcctaagta ctctattgat atggccattc cgactcctcc tgcccctgca
    36301 atggcagtta gacccatagg tttattttta tattttaatg cctgcataag atgaaggata
    36361 ttaataattc ctgagcaggc ataagggtgt ccgtaagcta atgcccctcc aaaaatattg
    36421 aatttttctc tctcttcagg ataataatga ttaaataaag catcaatcgc tgcaaatggt
    36481 tcattccatt caattgcatc ataatccgat attttagtat gagtttctgt taatagtttt
    36541 tccgtagccg tgtgaaccaa ttctggacta agcttgggat ctcctgctac ttctacaatg
    36601 tgaacaatcc ggaattctgt tttctgactc tgaagcgtta gaaatgcagc agcatcgtgc
    36661 attaaacaaa catttccaat agtgagcaaa ggtgaatttt ccatcaatct tggtaatttt
    36721 tgaaaaaatg tttcttttag ttttctaacg ccttgatctc gcatcccttc cattggtaag
    36781 attacctctt ctaaatagcc accttgttta gctgttaagg cgcgtttatg gctcaagaat
    36841 gccaatttat ctaatatttc tcttctaaaa ccatattttt gacagactct ctgggcccct
    36901 tctaacatta cagtttcagc gtaagagtca ggagaaaact gagcaacggt atattctccg
    36961 ttacgattat cttctttagc gtaacgtctc ataggttgaa gagaactact ttcaatcccc
    37021 ccaacaagaa ctttttcatt aatatcagca ctgattttta gataaccaaa aaacaaggca
    37081 gaacttgatg aagcacactg catatcaatc gtttgtactg gaatatagga ttcataatca
    37141 gaaaaaagag tcatcaaacg accaatattg cccccagtac caactgtgtt cccacaaata
    37201 atactatcaa tgttagattc tggttctatt ttttttattt gatttaagag gtgtgctcct
    37261 aaaagttctg gacggtaatg tttaaattgc ttccctttta taccgatagg tgttcgtagc
    37321 ccaaaaccaa tataaacatc tctattcttc atatgtcccc ctcacacata atatttgatg
    37381 ctgaaaaata atcactgtgt tatccactac ttgcactgag aattcttgat tcacccttac
    37441 tggagataaa aaagtcaaat gaatctcctt tatttttcct tttctaactt ccattatttt
    37501 ttccaataat agagccgcag gcacaatagc tgcttcccct tggtgaaaag gatttttatc
    37561 tcgtactaag ctagtgaatt tttgacattc ttctattgaa ataagactaa ccttctcttc
    37621 ccttgcagga cagtcactgg atgctagtga cattggctta agttttggta aattaagcat
    37681 cctcaaatgg taaatccatt gctgatcagt taccgttacc tcaaggattc tcttagaaat
    37741 ccatttaata ttatcagatg agccatcctc tctttgatac aaactaagtt caacaattac
    37801 aaaattaagc ggcaaatctc ggcacatagc tatcgctttt ttaaatagtt ttttcatctt
    37861 atctcccgtc aaaatataag accagtatat cataattttt atattgttaa cttataatat
    37921 agattaagtt tacaatatag ttttttttat gctattatta atttcgttaa aggaggctat
    37981 catgtcattt caaacaaatt atatccattt ggctgacgaa atattatctg gaaagactag
    38041 catttcttat gaacaggcac tggaaattct aaatagcgat gagaactggt gggaaatcta
    38101 cgctgctgcc ttatacctta aaaatcaagt tagccgaaat aatattcgcc ttaacgtttt
    38161 attaagtgct aagcaaggac tttgtgcaga aaattgtggt tactgttctc aatctaaaga
    38221 aagcactgct gacattgata aatttggtct gcttcctcaa aatgtcattc taaaacaggc
    38281 tattgtcgct catcaaaatg gcgctagcgt cttttgtatt gcgatgagtg gaactaagcc
    38341 tagcaagaga gaaattgagc agttatgtca agtcatccct gaaatcaaaa aaagcctccc
    38401 tctagaaata tgccttaccg ctggtttttt ggatagagaa caacttcatc agctaaaaca
    38461 agcaggaatt gaccgtatca atcataatct caacaccccc gaagaaaact accccaacat
    38521 tgcaacgacg catagcttca aagatcgttg tgatacttta gaaagaattc acaatgaaga
    38581 cattgatgtt tgttctggat tcatttgtgg tatgggagag agcgatgagg ggctcatcac
    38641 attagctttc agactaaaag agctgaaccc ctattctatc cctgtcaatt ttttacttgc
    38701 tgttgaagga acacctcttg gaaaatataa ctatttgact cccattaaat gtttaaaaat
    38761 tatggccatg ttgcgttttg tttttccttt caaggaatta agattaagtg ctggacggga
    38821 ggtccatttt gagaattttg aatcattagt caccttactt gttgactcaa cttttttggg
    38881 aaattaccta acagaggacg gtcgcaatca acataccgat attgaattct tggaaaaatt
    38941 acaactaaat catactaaaa aggaattaat ttaaatgaat acaagaacaa ctacctatat
    39001 tgcactcatg gttgcactac tcattgtttt aggttttatc ccagggatcc cattaggttt
    39061 cattccagtc ccaattgtct tacaaaacct tggtgttatg ctagcaggtg ctctgcttgg
    39121 tagtaggaaa ggtttcctag ctgtggctat tttcttactc ttagttgcta ttggtgcacc
    39181 atttttacca ggagggcggt caggtctagt aacgctgttt gggccaacag caggctatct
    39241 gctgacttat ccatttgcag ctttttttat cggcctaggg cttgagaaag tgaaaacaac
    39301 aaaactctgg gttcaattcc ttattatttg gatatttggc gtactattga ttgatatttg
    39361 tggtagcatc gttctttcct ttcaaacttc tctcccctta actaaatcac tattttctaa
    39421 ccttattttt attcctgggg ataccttaaa agcatccatt tgcttgatta tttatcgtaa
    39481 atttgcaaat agattaactc atctatacaa ctaaataaaa gacagtacct aaggtactgt
    39541 cttttattta tagagagtca ttatcctaca ctaacacatt ttagtcatta attaacgtca
    39601 aaaagttttc aatcattttc attccatcaa tactcccgat tgactcagga tgaaactgca
    39661 tcgcatatac ctttaagctg gggcaatgga atgccatgat ttcattatcc tcatttgatc
    39721 gtgcaataat agcaatattc tctggtaaat cagtcactac caatgagtga taacgcataa
    39781 cttgggttgg actatcaatt cccttaaaca aactagtatg gtcgtttatt gttacccaac
    39841 tttgtttacc atgcataacg tgatttgcta aatttaatcg cccacccaag cactcagcaa
    39901 gcgcttgatg ccccaaacag atacctagaa taggtttctg tcctataaac tgattgatta
    39961 attctaatat tttcccagca tcctttgggt gtcctggtcc aggtgataag acgatagctt
    40021 ctgcgctctc agctaataaa aaaaggttag gatcatcatt ttttatgacg aatatttctt
    40081 tatagacact taaatattgc tttaaattat aggtaaaaga atcgtaatta tctaccaata
    40141 gtaacatttt atagaccttt ccttgtactt tgacctcatt atacactaat tgtaaaatat
    40201 aaaaaaggct tagaggaata actattccaa cctctaagcc tcacagatta tgccttttga
    40261 atagcctaaa ttttctagac ctccatcatg ccggcttgta attggtacat acgataataa
    40321 gtcccttttt ggtctaataa agactcgtga ttcccacttt caattatctt tcctctatca
    40381 agaacataga tacaattagc atcttgaatg gttgataagc ggtgagcaat ggcaattgtt
    40441 gttcgacctt gacgcatttt tgctaacgaa tcttgtacaa tctgctctgt ttccgaatca
    40501 atatttgctg tcgcctcatc taagattaaa attttaggtt tactagccac agttctggca
    40561 aacgctaata gttggcgttg tccagttgaa aaacttgacc cacgttctga aacagctgaa
    40621 tcgtacttat ctggcaactt ctgaataaat tgatcagcat caacaaactc tgctgcgtcc
    40681 tgtacctctt gatcggtgat gtcttggtac attttgatat tagatttaat agtgccatga
    40741 tataaaaagg ggtcttgtaa gacgagtcca atattctttc gcaattgttc ttgtgagtaa
    40801 tccctaatat ccttcccatc taataaaact tgtccagatt gaaattcgta aaacctcata
    40861 aagacattaa taatagatga ttttccagaa cctgtcgctc ctacaaaagc aatagtttca
    40921 ccttttttaa cactaaaaga aacattatca aggatttgtt tttttccatc atatgagaat
    40981 gatacatttt taaattcgat atttccttct ctcacaaaag cttctgtatt tttttggctt
    41041 ggttcaaaac ctgtttcatc aatcagatca aacacacgcc ctgctgatac cattgatgtt
    41101 tgtaaggttg aaaaattttg cgttagttca attaaagggt caaagagacg attaacgtac
    41161 tgaataaaag catacattaa tcctgccgta agacctcctt taactcctgt aaatccaaaa
    41221 taagacataa gaacagcata tgctaggagt tttaaaagag acatcgccgg tcttaagaag
    41281 agactatcaa gagacataga acgattggca tacacaacat gctctttgtt aatttcctca
    41341 aattcagtct tcaagcgctc ttcttgacca aaagcctgta caatgcgaat tccttcaata
    41401 ctttctgata atttactgtt gatatcacta agtaaacttc tcgttttagc aatgacagtg
    41461 actgattttt tccgatagac attcactaat ataaagataa caggtaacaa aagagcgacg
    41521 agtcctgtta gtttaatgtc tagcatcaac atagtgtaca gagtaactgt aaaaataaat
    41581 atcgccgaga taaaacttga taaaataccc gaaaacatat cagatattgc ttcagtatca
    41641 ttagtaatac gtgacacaat agatcctgcc ggtgtcctat caaaataaga catgcctagc
    41701 ctttccatat tagcaaaagc atctctacga atatctctaa caatactata agaaacacgc
    41761 gcaaaaaaga gattcccaaa atattgaatt aaggtctgca agacatacat tgaataatat
    41821 cccactaaaa taagaactgc tgtttgatta acatttgtca gatagtgatc aataaaatgt
    41881 gaagcaatta aaggaataat atttttaaca acagtcgtca acaataagag agatagagct
    41941 aatactgtaa accatttata agggcgtaaa taggagatta atctcttaaa gacttgccat
    42001 tgattagact tcatcaaatg cttcctccat ttctaattgc tgtgaggcat acgtttcagc
    42061 ataccaacca cctttattta gcaactcttg atgttgacct cgctcaatga ctctgccgtc
    42121 ttgcataact aagataaggt ctgcgtgcac aacagctgat aaacgatgtg ctgaaataat
    42181 agtcgatttc ccttgacgat tcgttttaag gttctcaatg atagcatgtt ccgttttagc
    42241 gtccactgct gatagagaat catccaaaat aagaatatct ggatctaaaa tcatggcacg
    42301 actcatcgca atcctttgtt tttgtccacc agataatgag actccttttt ctccaattag
    42361 agtctcaaat cctgctggca tctgttcaat gtcatcgtaa acatgagcca atttagttgc
    42421 ttttttaaca gcattgatag atagagttgg atttccaaag cgaacatttt ctaaaatact
    42481 ggtagcaaat aaaaactgat cttgaggaac ataaccgatt agttgacgta actcagacaa
    42541 tcgataatca cgtatatcat gtttatttaa agtaattttc ccctgagtca catcatgttc
    42601 acgtagcaat aacttaataa gacttgtctt ccctgatccc gtttgaccta ccaaacctaa
    42661 ggtttgacct ttttctaagg tgaaatgaat atcggctaag gtttcttcat tgtcgtatct
    42721 aaagaaatca atatcatatc ttaatgttcc attgacaaca ggtttgatag gatttaaagg
    42781 atcagttata tccgattctt gctcaagtag actattaata cggttataag aaacactacc
    42841 acgctgtacc atattgaaca agaaaccaat cgccatcaag ggccatacca acatatctaa
    42901 atacgtcaca aatgttacca agtcaccaac agtaacttga ccttttgaaa tcataaaagc
    42961 tcccatagcc aatgttaata cgtaggaggc acctataaaa agaagaacta aaggatcaaa
    43021 catgacatca taagtcatgg tccgcatgtt cttcacgaaa gtcatctgat taacctcttg
    43081 aaaagaagca atttcttgtt cttgataacc aaatgattta gtcactttga cgccagagac
    43141 actttcttgc actttattat ttaattctga aaaggctgcc tgagattctt tgaaggtttc
    43201 atgggttttt cgccccaatt tactagttgc taaggccatt aagggcaaag ggataaccgc
    43261 aattaatgtc atttgccacg aaatagtaaa gaacatggtg ataagcgtta ctaatgctgt
    43321 gatagaggca tccactgctg acataactcc tcctcctgca agacgtgtta gagaattgat
    43381 gtcgttggtc gcgtgcgcca ttaaatcacc tgtacgatat ttctgataaa aagaaggaga
    43441 catttttgta aaatgttcaa ataaacggta tctgacaact tggcccaatt tgtaagaagt
    43501 ccctaaaata tacatacgcc aaatataacg cagcccatac atagctaaag ctgacaaaac
    43561 caaacctaat aaattccata gtaattgtgg tcttgttaat tttccagttg taatagcatc
    43621 aataactaat cccataattt taggagggat aagatttaac accgcaacca aacttaatga
    43681 caaaatccca ataagatagc gcttcttttc ttccttaaaa aaccaccaca aattttttat
    43741 aatagacata tttttccttt ctcaaagatt gaaaagcatc ataattaagt cagaagacca
    43801 aatcaatcag aagacatgaa aagtgtttta aaattctcat ttaacaactc tctattatgc
    43861 ctctataagc ttactttcgt caactaaagt gattcttttt aaatagtaaa aggcttgaac
    43921 aatgtcatag cctttacatt attttcattt tctaatagtt agaagccaac cttttatgtt
    43981 tcgataagtc catagggcga aaggaccgcc taaggtattt gtccatagat cgtcgatttc
    44041 aaaaacccga ttagcatcta ttaaaatatc taaaacaact tgagtacact ctatgaaaag
    44101 agacatcaag aaagcaagta atataacgct tttatatttc cgtaaacttg gctttaggga
    44161 tagtaaccct ataatgagag gaaacagcag taaaatattc actacatttt gaccaataac
    44221 ccaaaaaatc tctttaaagc tagttagctg atctaaacta cgaaaagaat taaatggtac
    44281 taaaagagct acaattcttc cgaagcgctg aacattcgga gttgataccc cctcaatcat
    44341 aatttgaggc ccaaaacaca tcctgacaat aataagacaa tagataatca ctaacatcca
    44401 aacgaaacga cgactaatct ttgtcaattc cccatcttca gtaagtaatc ttttaagcat
    44461 tactgcccta ccttcgcttt ttgacgatcg cgtttcattg tgtttgaacg taattgtccg
    44521 caagctgcat caatgtccgt accatgttct tgacgaacaa cacaattaac tccatttttc
    44581 ttcaaaacgt catagaaagc ttctacacgc tctttaggac tacgactata ctggtcatgt
    44641 tctgagacag gattgtaagg aattaaattg acataagata atttgcgaat ttttttcgtt
    44701 aaatctgcta actcttgagc attttctggg gtatcgttga caccatttaa cataatatac
    44761 tcaaatgtta cacgacggtt agtcgtctca atataatatt caatagccgc aaacaacttt
    44821 tcaagaggga aagaacgatt aatacgcatg atacttgatc gtaaatcatt atttggagca
    44881 tgaagagata ctgctaagtt aacttgtaca ccttcatttg caaattcacg aattttatgt
    44941 gctaaacctg aagtagacac tgtaatgtga cgtgcaccaa tagctaatcc attatcatcg
    45001 ttaactgtac gcaagaattt taatacattg gtatagttat caaagggttc acctattccc
    45061 atgaccacaa tgtgactaac gcgctcacct tgtccacgtt catcaaaata tttttgaaca
    45121 agcataattt gagcagtgat ttcgccattg ttaagatcac gttgtttctt tatcaaacca
    45181 cttgcacaga aagtgcaacc aatgttacaa ccaacttgag tcgttacaca tactgataaa
    45241 ccataatgtt gacgcatcaa aaccgtctca atcagcatgc catcaggtaa ttcaaaaagg
    45301 tatttaacag ttccatcagc agattcttga acaatacgtt gttttagagg attaacaaca
    45361 aaattctcat tcaaaagtgc aataaaatct ttggaaatgt tagtcatttc atcgaagctt
    45421 tgcacacgct ttttatagag ccagtcccag atttgtgaag cacggaattt cttttctcca
    45481 tgttcaatag cccaagctat tagttcatcg cgagttaagc tataaataga gggtttatag
    45541 tcgggtgtat cagttttttt aggcataata ttttctttct tcatggtgga ataaacggtt
    45601 acagtttatc cctttcttat tttttcttac gaataacaaa atgctgcttt gcagaggtca
    45661 tttccttatc ttgctcttct ttaatatgac gttcttgacg gcgttttgtc tctttacgct
    45721 ttttacttgt aaaatgagca ttggcatttg actgggaacg attatttcta gtttgtgact
    45781 ttgatgattg tgatttggat cttgacttac gacgcttatg aggtctttca tcatcaaatt
    45841 taatatctct aggatttgga ttctccaaca caaaataagc acaaccaaag ttacaatact
    45901 ctttaatata atcttctaaa cgtgaaatcc ggctattttt tctaatcgta ctagcatctt
    45961 tgtaaaagcc tcttagcctc aactgctcgt taccccaatc gccaacgata aaatcatatt
    46021 ttaataaaat ctcagaaaaa cgttgtccaa agacagtcac atcaaaagct gatttttcat
    46081 taataacaag ttgaaattca atatcatcac ttttaacgat attttcaaag tgaatgaact
    46141 gtgggccagg atacttatta tagttaagca tctctggtgt cacttctttt cgcataatac
    46201 tcctttctct aaaatcattg agaagaaacg actagcctct actctttatt tatctatttt
    46261 tcatttatag tgtcatattt attaaataat ctgctaccac atctctgagt aattcaggaa
    46321 aaagcaagtc tacttttttc gtttttaaac actcaaaata cgatgcaaaa taatactgat
    46381 caacgattac aagaatcact tcttctttgg catcaatatc tttctcatta tacactattt
    46441 ttccattttt gtaatcaaat cccgaatgta aaacgtctcc aaagtacttg ccacgaaagc
    46501 ccataccatg aatcttttgt tgcgctaaaa attgaccttg ttgataaata gtttcataaa
    46561 tctctaaaag ttcttgacta ctcactgtca atttagcgag acgcatttga tgaggtaatg
    46621 attcgtggag atttttcctc gaaaagtctt ttctaaatgg ctgaacaatt aagccagaat
    46681 tgataatagc aacatctgca cctgcataaa acagcatcgc atccatgact agttgacacg
    46741 attcttctaa tgataatggt gctggaaagg atattaaact ttcttttcta agctgttctt
    46801 taccttctga ctcaatcttc ttagcccagt cactgtccga aggataacca gttagttgtt
    46861 ttgtatcaca tgttgagatt aaaatatctt ttagtgtatg attatcaaaa gttatatcaa
    46921 tactcccaac aaagcgacca tatttaccag ctgctgcaag atatgtaccg ttaattagct
    46981 ccccttcctc aaataaatgg tgtgtatgtg cacctataat aaggtcaatt tctgaaaatt
    47041 cttgagcaat ccttgtatca aaacgaatac ctaaatgact catcaaaata cgacaatttg
    47101 cctcttttat ctcatttatt tgtaaatggc atttaagaca gtcaattgga tcttctatca
    47161 tccacccatt aggttcatag gttttataat aaggaaaagt ataagccaaa aaggcaagct
    47221 tagttccttg ctgtgtctca taaataatgt aaggttttgc ccaactcggt tcgattgtgt
    47281 tatcttttag attgccaaca ataaccgtaa aatctgaatc tttgtagact tggtccaagt
    47341 cttttttgga taaaccaact ccttcattat taccaatagt agcaagctca atacccagtt
    47401 cattcatcaa ttggatatta gccttgcccg agcttgcttc tgtcaaagga tggcttttat
    47461 caatattatc ccccaagtca agtgaaatcg tctctattgg ctgcgcttga ttatcatgaa
    47521 aaaatcgttt taccttagga aaattctcaa aatgagagtg cagatcattt aaatgtagta
    47581 ttcttataag ctctgtcata ctatcatttt agcactttga tagtcaaatg tcgcaaaaca
    47641 aaaaagccga gcagacgccc gaactctttg agtttatttt gtttttttca aataatcaat
    47701 agcttgctga acattttgaa ctggtacaat tttcatcttc gtacctaatc gcttagctgc
    47761 tgcttttgcc tcttgataat tcgtctgaac ctttgttttg ccttttttag catttttatc
    47821 gatcggatta ttaggaacaa agaaaatatc cattcctttt ttagccgctg aaacaacttt
    47881 caaacctgct ccaccaatat cgccaacatg cccattttgc tcaatagtac cagtgcctgc
    47941 aatcttacgt cctttacgta aatcttcttt aactaattgg tcgtaaatcg ctaaagtaaa
    48001 cattaaacct gcacttggtc ctccaacacc ttcagtatta aagtccacag gaacatcaga
    48061 tgatacttct gtatggtctg tcaaaccgat accaatacca tttttaccgt ttgatagttt
    48121 aataacttta ccaactgatt ctttcttttt accttgactt gtgtattgta cctttacttt
    48181 atcacctaga tgaagagctg caacatattt gataagttga gaagaatttt tgaaactttt
    48241 gccattaact cctgtgactg tatcagctaa atgtaatcta tctttaaatg tcgaattttt
    48301 agctaagtta agcacataaa ctcctttata agtaagagat acttgcttat tagctaactt
    48361 taaagcttga tagacggact cattttgaga agtctccatg tagaattgat taattctgag
    48421 ataatcgtca ttactaaagc cacctgttgt ctcctcttta ctagataatt ctgtaaaagg
    48481 agttaaccaa gcataaaaaa cctgagctgg cgttgcttga ctaacagata ccgctacaaa
    48541 attgtacgaa cccttagctt tatcagcctt tttattcact ttcaataccg aacggatatc
    48601 ataagctcca cctggcattt ccaaataata aggtagtctc accacaaggc ttgctaatac
    48661 taacaataaa aaagcaaaac ctataatcca ccatttaagc agacctagta aacttttatg
    48721 ttttcttttt ggatctttat tcttcactca ttttctccac ttctctaaca acactctttg
    48781 gaacaaaagg cttcacagaa gccttaaaat gtatcaattc acggatgcga ctagatgata
    48841 tcggactcaa agatggcgac gttgataaat aaactgtctc aatatcatct gccaactgtt
    48901 tattaaaaaa ctccaaattg gcttcataat caaaatcctg gctattacgc agacctcgaa
    48961 caaaatattt tgctcccact tcccttgcta agtcaacagc tagtctatct tgcgccacta
    49021 aaactgaaac attcttaaac tgacggatag cttcttctaa cattttttta cggcactcaa
    49081 tcgaaaagta accttgcttc tcaaggttat aaaataaccc aatgtaaaca tgatcaaata
    49141 gatagctggc gcgctcaata atatctaaat gtccattagt aacaggatca aatgaacctg
    49201 taaataaagc tttctttgtc attcgtcgtc atctccacaa taatacaacc ataaaaagac
    49261 taccacagga atcaggcgaa caatgtctat tgcctcaaaa ccgttagcta tccaattgat
    49321 aagatttggc aaaaaagtaa tcattgccat taatagatac aatggatttt ttgttcttga
    49381 taatgaacaa actccttcat catttttgaa gtgataatgt tttagcaact cttctaaata
    49441 gtgaaggcgc aacaacaaat aaatcaatat gactaagatg agaataatca tcactaactt
    49501 gtaatccata ccaaagcctc ctaacgaacg tagaccgtta ctttagaaat gccataaatt
    49561 ttctgtttcc atatcccaaa agaagcaatt tcttcaggta aatcaactga tttgtccgtt
    49621 tcacaaacaa tcatgatatc atcgcctaat aaacccttgc tatccatgat ttggatttgc
    49681 tttacaattt cttccttagc atatggcggg tccaacaaga ccaaatcaaa ttgtcccgtt
    49741 aattgttcta aagcacggtt ggcttccatt tttaataatt gaaattgctc cggactctta
    49801 gtcattgcaa tattttcttg aataacgacc tgcgcacgcc tatctttttc aactaggaca
    49861 gcttggtcca ttccccttga gatcgcctca atagctaagc taccactgcc agaaaaaagg
    49921 tctaaaacac gaccaccttc aaaaaaagga ccaatcatat taaaaatagc gcctttaacc
    49981 ttatcagttg ttggacgagt tgtttttcca tcaagcgttt ttaaaggacg ccctccaaat
    50041 gttcctgcta ctactctcat aaccactatt ataacaaaaa ataaggtaga gttatcaaaa
    50101 aaattgctta taacaaacag agaagatggt atctcttttc cttctttttt gatatgataa
    50161 gctagcagta agagaaacga ggttcccata atgattaaaa aattattatt aagtttaata
    50221 gccatattat gccttacaac gctaaaatca aacttaagta gcgctgacac catgactatt
    50281 tctccacaac aataccaaca tatgataaca aatagtgact taatcgccat cacagataag
    50341 gcgttagtaa gcaaaagtca acaatctgaa gcgactattt ttataggacg tccaacttgc
    50401 caatattgta gagctttctt accaaaatta ttaaaatctc aagccacact tcattcaaaa
    50461 atttactacc ttgatagtca aaaatacaaa ggggaacgat tgaagagttt tttcaaaaag
    50521 catcatatta ccactgttcc aaatttagca cattatcaac gaggaaaaat gactaaatat
    50581 cttgttcaag gaagccaagc tacacctcaa caaatccaaa ctttcttaag ccaaaaataa
    50641 acttattgtt aaaatcagcc aaacaaaact gcttggctga ttttttataa tatattttcg
    50701 aaggtatctc tgacttcttt tggccagaca cttgactgta cttctccaat atgtttttta
    50761 cgaagcaaga acatagctaa tcgtgattga ccaattcccc caccaatagt caaagggaaa
    50821 aaaccacgta atagcgtctt atgccactca aactctaaac gatcttcatc accggtgaga
    50881 acaacttgac gtttaagagc atcttcatca actcgaattc ccattgatga tagttcaaag
    50941 gcagttccaa gctgttcgtt ccatactaaa atatcaccat tgagaccttt aaaaccattc
    51001 tctgatggtg ttgtccagtc atcatagtcc ggtgcacgac catcatgtgg tttaccatct
    51061 gctaactctc caccaatacc aatcaaaaag actgcgccat actccttagc gatggcgttt
    51121 tcacgctctt tagggcttaa gtctgggtat ttttctacca attcttctgt atgaataaag
    51181 gtaatacgct ttggtaaaat ggattcaata tcaaaacgag cttccacggc aagctctgtt
    51241 aggcggatag ctttgtagat tttttccaca gtttctttta ggtagtccaa attacgtcta
    51301 ccatcaggaa tgaccttctc ccaatcccac tgatctacat aaactgagtg tgttggatca
    51361 agagaatctt catctggacg aagagctttc atatgaacga aaagaccttc tccctcatta
    51421 aacccaaatc ttgccaaagt atgacgtttc catttagcca atgagtgaac gacttcaaat
    51481 tcagcttcag gtatattaag gactttcacc gatacaggat gttcaatacc agataaatta
    51541 tcttgcatac catccccgac ctgactaaga ataggtcctt gcacttcgac gatttctaac
    51601 ttatcaatca aatattgtgt aaaagtattt ttgacaaaag aaatctcctg ttgctgatga
    51661 ataaagcttt tcttcatatg ttcctcctta aattataaaa tgttagagct attatactcc
    51721 ttcaagtaac aaaatggaag aggagttgta gaattattct gactttttta caagtttttc
    51781 tgaaaatctt tactctagaa ttgtttttgc catgacttgt cgcaattcct tggtcattcg
    51841 ccgtggtcct ctcacagtct taacgccgtg ttctgctaaa aatgctacag gataggtaga
    51901 atcttcaaat tgccttatca gtttgagtcg catataagct ttgacatctc ttgctcctaa
    51961 gtaagtactt gttttaaggt cacgttctat tacttgacag acatagcgca aagcacgaat
    52021 aggtgctgta atataaataa acacataatc tcccaccgca atctttcctt tttgagtcca
    52081 atcaatcaat tgatgctctg caaactcctt atcaatatcg taaaatttag gattagcagg
    52141 aataatccaa aactctggct cagaatttga ttttttattg ggagaaacaa gcttacgact
    52201 atctgatacc aatttaaaaa tttctatatc agacaaagta ttgtctaaaa cgatagatac
    52261 ccaagtcttt ttagacatat gataagaagg ataaatacct tctttttgta agagtatttc
    52321 catatcttga ggattgactt taatattcaa aacttcaacc tcatcttctg acagttgagc
    52381 tggaacattt tctagtttcc ccatcttcaa tggaaaaagc agagcatacc atttacctga
    52441 tacacgataa gaagcatatt gtgggtgctt cgcaaatgga taatcaactg aatcttcaaa
    52501 agttttagct atcttctccg ctaaacgatt actttgatct ttggtaaata ataagtcctt
    52561 acaacaagaa tctgaaatat cacctaatat agccatataa gccgatcgta cttcgccaac
    52621 aaacgaacca ttataatttg ctgctcgaag cggtaaatat tcttcttcaa gtgccgaatc
    52681 aataacctta ccagctatct ttccatccaa tgaaatcgtg ataatagctt taaagtcatt
    52741 atccataaat gtttgacaat agatatactc ttggtcagat ttgataaatc caaactcctc
    52801 aagcgatgaa aaaataaaac gttttttccg gaaaaaatct gattctattg acatagtaac
    52861 ctctctattc actgtacatt atcctctatt atgtcgtttt aagagctaga aagcaagctc
    52921 aaagtcaaag atgaattcta gtttctttgt acaaaaaaag catgttgcca tgcttaaaac
    52981 taaactaatt gatagcaccc tcttattaat tctctattta tgtttgggat gattatcctc
    53041 tttatggaca taaatgtaca taacaataga gagcataata gtaatgatag tcgtaattaa
    53101 aataatcaat acccagacag catcaacact gtcaaaaggc aacttaacat tcattccata
    53161 aaagctggta accatagcaa taatggaaat accaatggaa ataattgtta aggtagttac
    53221 attgtcgtta aggttattgc tgaggacatt attataagaa gaagaaagtt cttggaaaac
    53281 ctgtgaattt aaggaacaca tgttagataa ttgtcttgct tcaataatag catcttgaag
    53341 ttgcatcttt tcatttctag tgttctcttt atcttgctca atgttttgta aatcggtcag
    53401 catttcaaaa ttttgcttag ttcccatcat taagtgtact gttccacttt gtaaatttgc
    53461 cagccttgcc aagttcgatt tgtttggtct tttacgcaaa tcattgatta gtttatctct
    53521 ttcttcagaa atagtatcta aaacatcaaa atattgcttt gagaaagcag tcactaatga
    53581 taataagagg tgtttcggag aatctacctt ttcaacacga ttaagtgctt tttttacaag
    53641 gttaatattt ttatgattag ctaatacaaa caagccatct tctttcaaaa atagagtcac
    53701 tggtatcact ctcggcacat gacgaacatc atcaacgtga ccttgcgtat ccaaaagctg
    53761 gtaaacaagt gtcaaatgcc cctcaatatc acgtacgaaa gaagactcat tttcatcacg
    53821 tgcataagcc agcaattctg aaggtaaaaa tctctcctct tgcaaggata ggtcgtcaac
    53881 attaattatt tcaaatccaa aatcgctcga aaacatagaa gcacctcact ctaaacttgt
    53941 ctacattatg aaacaaaaat cgagaaaaaa caagtagaaa acccttttaa atctaattaa
    54001 ccttatgaga gcaaaacaaa aaaggactac taaaataaca tctagtactc cttattaata
    54061 tgctagatag ccagcatcat ttcccccatg aacggtataa gtcttctata atcttaacac
    54121 ctgacagcgt caactgaggc gtcatgatat gacttaccct ttgcccttct aaaatcattt
    54181 gactaacatg ataagcttct ttctcaaaat cactaaccat gtcaacttga atcgtttgcg
    54241 cactagtatc atttctcacc aattttgcat gagtggtttt ccaaaaatgt ggaatgatta
    54301 gacgaccttc agttccataa ataatcattt catgcggtaa atttaaacgt gttgtcaaga
    54361 agatatctac gaggacccca tttgataatt gcaagagtag tttggattga ctatcgcttt
    54421 gaccttttgg aaaagtcgca gtcccactag catgagttat agtcgcatca aatagatatt
    54481 gtaaataaga cagggcgtag ggcgccataa aatgcactgt tcctcacctt tgacataaaa
    54541 actcctttga cattgataga gttattatag cataagaact agcaatttat ctttttaggg
    54601 attaatacaa tttaatcgca tctctgttaa aagttagaca attttaatag cataatttga
    54661 aagatttaaa atagttgaaa ctacacaccc gcaaaggggc gatgataatg aattaataaa
    54721 aaaataatca ttattaattt caatccacgc acccgcaaag ggtgcgactt tagccgtgtt
    54781 attaaaaaag gcaataatct tttatttcaa tccacgcacc cgcaaagggt gcgacggggc
    54841 aaggatagac ctttttaaaa ggaggtaaca tatttcaatc cacgcacccg caaagggtgc
    54901 gacataatta tggtagtctt atttttaata taaaataatt tcaatccacg cacccgcaaa
    54961 gggtgcgaca aaatggtata atagaaggag ctacaaagaa agatttcaat ccacgcaccc
    55021 gcaaagggtg cgacaattat tacgcaagaa cacagccaaa atatattatt tcaatccacg
    55081 cacccgcaaa gggtgcgact ttgaatgggt caccgcccaa ttctttaata cctatttcaa
    55141 tccacgcacc cgcaaagggt gcgactactt cacttcaata tctttaatat tcttgaataa
    55201 tttcaatcca cgcacccgca aagggtgcga cattcttaac ttgtactctt caaataacca
    55261 tttaaatttc aatccacgca cccgcaaagg gtgcgaccag tatttaaaat acttctaaca
    55321 gtatttactt ctatttcaat ccacgcaccc gcaaagggtg cgacggtaca cccatttcat
    55381 ttaaattttc taagagtatt tcaatccacg cacccgcaaa gggtgcgact aatattttct
    55441 aacattttca agctctacat ctatttcaat ccacgcaccc gcaaagggtg cgacatttta
    55501 tctcatttca ctcttagaaa atttaaatat ttcaatccac gcacccgcaa agggtgcgac
    55561 tttgaatggg tcaccgccca attctttaat acctatttca atccacgcac ccgcaaaggg
    55621 tgcgacgatt tggaagatac tgactgggaa tattatgata tttcaatcca cgcacccgca
    55681 aagggtgcga ctgaagagtt attacaaaag caacgagaaa acaatatttc aatccacgca
    55741 cccgcaaagg gtgcgacagt gcgcgattgc accgtttata actaaaacca agaatttatt
    55801 aaacaagttg gttcaataaa aactagattt tgactactat tttctttttt ttagcgcgaa
    55861 tactccgctt tttcatgtgt gacccgaatt cacatataaa tgtttataga agtaataccc
    55921 ctatatcagg atcataacta tcatttttgc caatagtttc cacacgattt tgccaatttt
    55981 taccaagtaa ataaaatcgg atactatctg atttctggtc gatgattgtc agcaactcgt
    56041 tttttatctc cacaaactca gctggagtca ctgaacactc aaaaacagaa ttttgtacac
    56101 gttgaccata atcaacacaa agttttgcga cgtgacgaag gcgtctccta cctgctacag
    56161 tcttcgtatt gacatcatag gttactaaaa ccatcataat tttactccta gattaaaaat
    56221 ggtggatagg attccaaatc accacgtatt gctttagata ataactgtgc ttgcacataa
    56281 ggcagtagca tcaacttaac tttctccttg gtgaaaggat gttcaactac tgtatgcttg
    56341 cgcttttgcc aagactcaat aaagattgta cgtccttttt ctgtcaaaat aacactacta
    56401 ttttccttaa tatcaaaatg tcttttattt aattgtccct tattaaccag agaaaaaaca
    56461 aatcgatcaa caatataaga acgaaactct tctaccaaat ccaaagctaa acttgctcga
    56521 ccaggtctgt cggtatgata aaatccaacg taactatcta atccaactgc ttctaaagca
    56581 gattgacatt cgtaggtcaa cagactataa cccaatgaca tcagagcatt gatacagtct
    56641 tgcggtggcc gtcgtgtccg accgttaaac cgaaatgtat ccttatccgt taagacaagt
    56701 tcattaaaag cacgaaagta ttgatttgct gcttgtcctt ctattcctag cagagtttcc
    56761 ttatcaggtg cagattgaat tagttctaac gcccacatca attcagtatt aatgtcttca
    56821 aacaattgaa tatcaattct atctcgatga tcacgtttaa aacgaattaa atattttcta
    56881 gaattagata cttttgccaa tataaagcgt ttggcatatt ctattgaaag agcatggtcc
    56941 gctaatcgat aatgttcacg tctaagcaga acattcccat tcgttgaacc tacaaagcga
    57001 ccacaaaacc gaccttgtgg agtatggaaa gaaaggttaa tttgtttctc tgtacaaagt
    57061 tcaattaagg atggtgaagc acctaaatat gaaaaacaga taataccatc aattatacga
    57121 tatgggaagc gatgaacaat tttcccctcc tgcttaataa cgatattatc tcgctcccgt
    57181 gtgagataaa aattgtcctg tgtgagatat aaagtattta acaatttttt cataaactat
    57241 cctcatttac caaagactgt ctaatgtaat tgtctacgtt acgtgttttc ttgctcagcc
    57301 gtggtttaca aatatctacc aaagaacaaa gaggacaatt tttaaaatac tcagctttac
    57361 ctatgatttt attatcataa taaaaatgca tttgttcagc caaatccaaa acttcttggc
    57421 gtagttcctc agtaatttca atagttttct tttgattcac gctgtgataa taaagacagc
    57481 ctttctcgat atagcaccca aaagtctcct ctaaacaaat tgtctgtgct actagttgaa
    57541 caatatctcg tctatctttt ttcggtttac ctcgcttata ttcaacaata caaggaagcc
    57601 acttccctcg ttttcccttt aaagaaaagc cattttcatc tttataaaat tcaacaacat
    57661 caagaatacc atatagaccg agaatttttg atgacacgtg catagcacgt gagaccaaaa
    57721 aatcttttcg cttttcttta atgtaagggt tatctgcttt agtatgtaga atctgcccat
    57781 gcgctgtcgc ttcattatca agccattgtt gctcaatatg aatcaatgcc cattgacgtt
    57841 tacaaaattg gaaatgttga atacctgata acatcaaata atcatcttcg gcatagacca
    57901 tattatagcc cttctaggat tttaaccgtt aaacctttcg cttcatattt agctagtttt
    57961 tcttgattta gatgaatttg ataagcgtca taagtgcttt tttcttctat tgattgatta
    58021 tactctaaca agtcaaagac acgcgcactt gaaacatttc ccaatttgct tgaatgagta
    58081 aaccaaaaga cttcgcgaac tcgcatagaa ccttctggac gtgcagacga tgcatcattt
    58141 tcaaataagc taaccaaaac ttctttaata gcctcagcat cttcctgaga aaaaccagtc
    58201 ttttcagcaa aataagcatt gatagaacct ttaagcacat aaataccata atctacaaaa
    58261 tgttttgtcc ccatagtatc agaagagcgg ccactattat tcttagcttc cataccattg
    58321 gtactacgtg taatttgaag gctagaaatg acaattggct ccaaggactt tgccatactg
    58381 attgaaatcg gtccgcgcac cccaattgat tttttcagat aagtaaaaac ttgtccaaaa
    58441 gcacgaacat caaaccataa tgcatttgct ttttcttcaa tttctttgtc aggtgtctta
    58501 gctgtaaaat gttgcgaaaa gcgtttttcc agtgaacgaa agtcgtcttc aatacgctca
    58561 ttagcttgca caaaaatagg tttccccata tcttgcaaac gattacgaat cttccgttta
    58621 atggagacat cactcattac accgtatcct ttagcatctg tacgaggcat gtttccgttt
    58681 aatggatcac catttgcatt tgcttctttc acttcaaagg tcactataaa atcaattttg
    58741 tgttccaaca tctttttact cctcactttc tgttacttca gtattttgtt ttggatagta
    58801 atagtttttc tcagcataat acccaaaaat aaagcggtaa tctaacggtt tctccattgt
    58861 ttctgtttct aatagaggat ttaaaagttc taaaatttct tctttttctt tttctaattt
    58921 cttccaactg ccacgacgat ttaatttcaa tgtttcttcg taaggcttaa ttttattctc
    58981 caataacatc atcaattttg ttggctgtcc agtataggct gtccaataac gttcagcatt
    59041 agtaatacga tcgctgccgt ttccctccaa gctataacgc aaggtctcaa ttaattcaaa
    59101 aattgctaat aatcgtccga aaagatagga acgattttga ttggtatgat ttagcatcgg
    59161 tgaaaactcc tccccatttt gtttgtgtaa aattgctaag cagacctgct caacttgata
    59221 ccagtgctta cgataacgat gtcgttcttt aacattgtta cctaattttt tgacaataga
    59281 ctgtggcata ggtttaccat caatcaaact tgtcactaat ttttgaactt gatcactttt
    59341 gaagttatca ttgtctaatt caaggaagcg atccctatca actccgtagg acacattaag
    59401 gatgtcatga aaggcagggg ttcttaagcg actcttccca gacttagatc gcgattccca
    59461 actgtatttt tcctgccact tgtcaagatt ggtgagtaat tgggacgctt gaatctgacg
    59521 aaaatatttt aaagcaattc tcccgttgct ggttttattc aaaatagcaa tgtaataagt
    59581 ggcatcgtta gcaaataatt tttgtccctt gatgaatgat ttaccaatcc ttttattttc
    59641 ttcagttgct aatgctataa cagagggcat atcctcatca tcatcttctt caagtccatc
    59701 atcaaagatt ggtgatacga tatccaaccg actatcattt gttaaatcat cactaaacca
    59761 gttgattaaa tattgagaag accctaacca agtactggta tttttatttt ctaaaaggta
    59821 cttgatcatt aaatgaatct tttcggaagt ttcatagcca acactaaaaa catcttcacg
    59881 ttctctaaaa cgtcctttat aagcttctct tttattacta acagagatga ttttagcatt
    59941 ccccatcaaa cctctatgct tatcggtaag ctgttcctct cgtccactaa tattacaaat
    60001 accaagattt tcacgattgg cttcaacgaa agaaatatag gattggtgta aagttttaaa
    60061 tgtcgaaacc gattcatttt taaagccctg gaactgatca attgaaaatt ctaaaaagca
    60121 agctgataaa tcaattaatt tttcttttcc agtggcatca caaaatgtga cttttaattg
    60181 attattttga taatcaggac caattaaaga atcgagaatc agtgtcagaa attctggttt
    60241 caaaataaat tgctgaacaa tggttaaaaa cctcttgacg tcaccctcct cacaataatc
    60301 aatccagtta ttaagttgct tatgaaaaga atcatactgg gactgtccca tttcagcact
    60361 ataataagca aatttatcga ctaggggatg cggcgcagga ttactacctg acctagcaac
    60421 agaatcagcc gttacaggaa aaataatcat ttgcttatct gtcataaatt ctgccttgtg
    60481 aaactggcca tctttgtcaa gtttgactga aatgatattt ttgccatttg actttaaact
    60541 cgtatgataa attggcagta aaaccggctc attatttctt ttttggtgat caaccaagtc
    60601 tgctagctct gctttttcat aagtcttcaa gagagaagta aaaaaatcca tcagttttct
    60661 cctttctcca tcgcatcata ctccatgtat tcatcgttaa ccgattttat ttcccctggc
    60721 actttaaaag cataactaga aagtgtgtta acaatatcgc aatcagactg tgttttaaaa
    60781 gtaatgactc cattttccat cacagtcttt gtaaagtatg atttcaatgg tgtctttttg
    60841 tctttcggat aggcaaagga atggaacatg attcccaagt cgatattgac accattataa
    60901 tacgacacag cagcctcata ctcttcttgg ctaatatcat ctacaagacc tacactctct
    60961 cttgtaccca aaaacacatc acgacgtcca cctttacgaa tagaacgctc cataatggct
    61021 tcatgtttag ctggaagtct gtctgagttc aaatcttttc tatcttcatt ccaaataaaa
    61081 tgaaatttga ttagataaac aacatcactc aaataggata cataacttaa atctgcacta
    61141 taatcatgta acagagccct gacaccctgt aattcggttt gaatctggtt aataacctta
    61201 acctctgtga caatattagt aaaggtcggc ttataataga tggcatcaac tataccattc
    61261 agcgcctgtc tagttggaac cgaataggat gcacgttccg atcccccttt tgtggctgga
    61321 tttgtaaaaa gggctcgctg accacttact ctcacgtaga agtctctaga tctgtacaag
    61381 atattttcct ccttttgttt ttttataatc taattttatt aaatagtcta aattgataat
    61441 aattattaat gatgattggc aggttaacaa gatcctctac caaccttttg ttaatctgct
    61501 ttcgttttga gttagaagta aaagctagct gaatcgtatt taactcctct ttccgtgtca
    61561 taatactccg acgtcaaaac cagaatctga ccatttagat aagattttgt tgcctcaagg
    61621 agaggatcgt tctcacggac attaaccgta aaaggttgta actcttttag taatgatttt
    61681 atcgtttgat aatcttctgc tgataaaaaa tgtgacgtac ctaattcttc taaataagcc
    61741 aattttttct cagcatcgcc ataaggtacg agaattccta tcatatcatt attgatgagg
    61801 ttcattttgg ctcctgctgt tttaaaagct tgttttaact tacctttgaa ctttttattt
    61861 gccgtctgat aagtattaag gcttaaatag tcgtagattg atatgttgtc ttccaaagga
    61921 taatccatca gtccttgatt attggcataa taatactcaa aaaagtcacg gtttaaagtt
    61981 gagatattga ttggagaccc aatcttataa agaatagatt ctgtggcttc ttttttagct
    62041 tttatttctg tcagcctaga aatattttcc tcttcattgg tcagattgac aagagtgacc
    62101 tgacctttat ctcgtttgcc ttctcgatta catcgtccag cagcctgaac aatagaatca
    62161 atccctgaat aggaacgaat gacacgatga aagtcaacat ctacacctgc ttctattaat
    62221 tgtgtgctaa tacaaataat aggggtatta ttttgtaact ccgttttgat cttagcaacc
    62281 aagtcaagcc tatgctgcgc acacatattc gtcgaaagct gatagatcgg tctatcagtc
    62341 atgtctttga gcatagtata gcagttatga accgtttttt tcgtgttaaa aatagcaaga
    62401 actgagtttt cctctcctaa aataagatta ataacatcag tcaagtaaac tttctgatca
    62461 ctatcatcaa attttctaag ctctgtcctt gaaaaaattt gtttttcttc aatcgttaac
    62521 tcaactattt cagctaattc tcccaagttc cctccataac agatacgatg gtcaatctct
    62581 gaagaatcat aagcaggttg tgttgctgta caaagaacga tggttgtatc cataacttta
    62641 tttaaaaaat tcatcgttaa attaaacaaa gtggtgactt caataggcag tgactgaact
    62701 tcatctagaa tcacaacact attaatcaaa cttgaaaaac gtctcaaatt agccgatttt
    62761 gttttaaaaa gcgtttggaa aaattgaacc atagaagtca agactacttg actgtcccag
    62821 ctatcaataa gataagctga caataaactg tccttgtcat catcttcatt agcctgttta
    62881 accatattgg aatggtgttc taaaacgcca aggtcacccg taacttttct aatttcggaa
    62941 gcattttgct caagaaccga aagaaagggc gttatgtaaa aaaatcttga tttgtcttga
    63001 tgaactaatt ggtgaaacgc atagcgcata ctaagattag tcttgccagc tcctgtcggt
    63061 aaatctaagc gatagatccc tttggaatcc cgtttacctc tttctttaac acgctcagca
    63121 atttctgtcc gaatagtatt caactgattg tttggtcgtc caaagctagc atatttttgt
    63181 tcaatggctg ctaaataaga atgttttaga gaggatcgct ctgttttatc cataggactt
    63241 atttcaagac cataggcatt tactgtgtcc aaaatatcag cgttttttaa gagcgacaag
    63301 taaagtctca ccatacaaga ctgataataa tcccactcac tattatcttg ccagtttaag
    63361 gaagacatag cttgttggta attatcaaaa gctttatcaa taagctctct taaatctttg
    63421 tagccgtaat cacacaattt ttcttctaat tttatagcat accctttaat atcttcgtga
    63481 tagtgataat catctaagtc tctgttgata cggtttttaa atttattaaa gctataatat
    63541 tcagcatcgt cagaacagta gcaaaaatca tacatcccat gatgagccga gatgacataa
    63601 cccaccattt cattgaatgt cattctctca tctttatcaa cccctcggct ttttagatga
    63661 ggcccaataa tagagcataa gtattttgcc cctgcataag aatggtcaac atgtcgattt
    63721 ggattattta ataatttatc ttgaaaggct cgatcagctt tgcctagatc gtggtataga
    63781 ctaattaaaa aaagcacatc tccttgacca attatagatg cttcctgtcg actaaaacag
    63841 gccacatgcc ataagtgctc atctaaagac tgcttttttt cagattcttg ttctattttc
    63901 ttttgataat gtgccaacgt ttccttagtc atcatcaacc tctctccctc atccataact
    63961 catatccctc aatgatatac taacattttt gaaaggaatt gtaaacggat acaatagtta
    64021 atgcttcccc caaaaagcct ctaaatgact ttccaaaaaa acaggaatct tgacaattca
    64081 agatccttgt taggagttct gtaaaaaaaa ttatttttcg gctaggcgta gtttaagtta
    64141 ggaaacttaa aactagctta attggaagat gttaaatttt tataacacct aacgctgaca
    64201 atgctgataa tatcgacctt tacatttggt acttattttt tgtcatctcg actagtaaac
    64261 gtgtgttcta aatcgccaag cattagtttt ttcagcacat acaacttttc ctttcattct
    64321 tgcggagata ccatcaaaat gctaaattaa atttaacatt gacaacaaaa cacttgttcc
    64381 ctaaacagta tcaataagtc aaccgatcgg tttaaaatac tactgtctta tcgtatgtaa
    64441 ccccctctat ctagttgctg gtttcaagta gtatgataaa acctacattt tatgcatgct
    64501 tactaccatt agaatggaat tgttatgacc aaggtaaagt attcgttatt ttttctcatc
    64561 tatcttgcac tactatatct aactagtatc tactttcctg ctatttataa agtcctcatg
    64621 tatcttcact ttctatgtct attcattttt agcattatgt tcctttataa tctgattgtc
    64681 attttaaaat atatctttac gc
//
LOCUS       DATRTN010000003        63371 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.003, whole genome shotgun sequence.
ACCESSION   DATRTN010000003 DATRTN010000000
VERSION     DATRTN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 63371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63371
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.003"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..2366
                     /locus_tag="VBE22_000146"
     CDS             <1..2366
                     /locus_tag="VBE22_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEO7201627.1"
                     /translation="GNKIIDSYETDVNPHESLDEHIVHLTGITDKQLVKAPDFGQVAH
                     HIYQLIEDCIFVAHNVKFDANLLAEQLFLEGFELRTPRIDTVELSQVFYPCLEKYSLG
                     ALAESLNIELTDAHTAIADARATAQLFIKLKAKISSLPKEVLKTILTFADNLLFESYL
                     LIEEAYQEADFVNPKEYYFWQGLVLKKEKAVGKPKKLSSDFQVNMALLGMDARPKQIV
                     FADLVKAHFNDQTTTFLEAQSGLGKTYGYLLPLLDQSQKQQIIVSVPTKILQDQIMAK
                     EIKHIQELFHIPCHSIKGPRNYLKLDAFYKSLQVQDRNRLINRFKMQLLVWLTETTTG
                     DLDEIKQKQRLESYFDQLKHDGEVTQSSLFYDLDFWKRSYDKVAQSQLVIINHAYFLE
                     RVQDDKDFAKGKVLVFDEAQKLVLGLENFSRGQLDISHQLQVIQKIIDSPIPLLQKRL
                     LESISYELSHAAELFYRHNSFEFSETWLKRLKNSINALEVVGLDDLQTFFTATYTNYW
                     FETDKVNEKRLTILRGAREDFLKFSEFLPPTKKTYMISATLQISPKVYLSDLLGGFSS
                     ISTEKIAHEKNANQKVWIDTSMPNILDLSPEQYAYEIAKRLQDIMTLKQPTLVLLTSK
                     QTMFMVSDYLDKWEIKHLTQDKNGLAYNVKKRFDRGESNLLLGTGSFWEGVDFVHRDR
                     LIEVITRLPFDNPKDYFIQKLSQSLTKEGKNFFYDYSLPMTVLKLKQALGRTTRREEQ
                     KSAVIILDSRLVIKSYGQTIMHSLGRDFEISKEKINKVLTEMAKFLI"
     gene            2452..3645
                     /locus_tag="VBE22_000147"
     CDS             2452..3645
                     /locus_tag="VBE22_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7201628.1"
                     /translation="MTYLSERVLNMEESVTLAAGAKARELRVQGRDILSLTLGEPDFA
                     TPKNIQQAAIEAITDGRASFYTPSSGLPELKSAINAYFERFYGYSLKPNQVVVGTGAK
                     FILYTFFMTVLNPGDEVIIPTPYWVSYADQIKMAEGKPVFVTAKEVNHFKVTVEQLEA
                     VRTDKTKVILLNSPSNPTGMIYKAEELEAIGNWAVEHDILILADDIYGRLVYNGNIFT
                     PISSLSESIRNQTIVINGVSKTYAMTGWRVGFAVGNHDIIAAMSKVVSQTTSNLTAVS
                     QYATIEALNGSQESFEKMRLAFEERLNIIYPLLCQVPGFEVVKPQGAFYLFPNVTKAM
                     EMKGYTDVTAFTDAILEEVGLALVTGAGFGAPENVRLSYATDLETLKEAVRRLHVFMG
                     SNEIN"
     gene            3666..5012
                     /gene="asnS"
                     /locus_tag="VBE22_000148"
     CDS             3666..5012
                     /gene="asnS"
                     /locus_tag="VBE22_000148"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEO7201629.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEVIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYSTYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYIAEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDDYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIKP"
     gene            complement(5052..5609)
                     /locus_tag="VBE22_000149"
     CDS             complement(5052..5609)
                     /locus_tag="VBE22_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7201630.1"
                     /translation="MTKSPARLISFISIAIAINLVGANLALFLRLPIYLDTIGTLLIA
                     VILGPWYAASTAFLSALINWMTTDIFSLYYSPVAIVVAIITGILIKRNCKPSSLLWKS
                     LIISLPGTIIASVITVILFKGITSSGSSIIAQFLHGIGLDMTSSLILVQVGTDYMDRL
                     ISLILVFSTITLLKKHSPNLINQRF"
     gene            complement(5606..6589)
                     /locus_tag="VBE22_000150"
     CDS             complement(5606..6589)
                     /locus_tag="VBE22_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEO7201631.1"
                     /translation="MNKEKIIIDCDPGIDDTLALMYAIQHPKLEVVAITITAGNSPVE
                     LGLKNTFVTLELLNRHDIPVYVGDNLPLQREFVSAQDTHGMDGLGENNFTLAQPIIFQ
                     EESADCFLANYFEHKNDTSIIALGPLTNIARALQTNPKLGKHCKRFISMGGSFKSHGN
                     CSPVAEYNYWCDPHAAQYVFENLDKKIEMVGLDITRHIVLTPNHLSYMERINPDVSSF
                     IQKITKFYFDFHWQYEHIIGCVINDPLAIAYFVNENIATGFDSYTDVACHGIAMGQTI
                     VDQYHFYKKDANSKILTSVNTNLFWNDFMTVLLHAQNSVIIEDLEMLGLNK"
     regulatory      complement(6733..6777)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            complement(6835..7248)
                     /locus_tag="VBE22_000151"
     CDS             complement(6835..7248)
                     /locus_tag="VBE22_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEO7201632.1"
                     /translation="MLYEVTSSNTQGVDGKVYLSNGKIVETNHPLNHLPGFNPEELIA
                     LAWSTCLNATIKAILEQKGFKDLKSRVDVTCQLMKEKQVGKGFYFQVNAVASIEKLSL
                     SDSKLIVNKAHSRCPISKLISNAKTINLDTVKWSV"
     gene            7402..8292
                     /gene="rapZ"
                     /locus_tag="VBE22_000152"
     CDS             7402..8292
                     /gene="rapZ"
                     /locus_tag="VBE22_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEO7201633.1"
                     /translation="MSDEQIKLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLVPK
                     FLELAAQSGDTSKIAMVVDMRSRLFFREINSILDSLEINDNINFKILFLDATDTELVS
                     RYKETRRSHPLAADGRVLDGISLERELLAPLKSMSQNVVDTSELTPRQLRKVISEEFS
                     NQDSQSSFRIEVMSFGFKYGIPLDADLVFDVRFLPNPYYKPELRDKTGLDTEVYDYVM
                     SFDESDDFYDHLLALIKPILPGYQNEGKSVLTVAIGCTGGQHRSTAFAHRLSEDLKAD
                     WTVNESHRDKNKRKETVNRS"
     gene            8289..9263
                     /locus_tag="VBE22_000153"
     CDS             8289..9263
                     /locus_tag="VBE22_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEO7201634.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRLEDVEITAVVTVADDGGSSGE
                     LRSVMQLTPPGDLRNVLVALSDMPKFYEQIFQYRFAEGDGDFAGHPLGNLIIAGVAEM
                     QGSTYNAMQSLTQFFHTTGKIYPSSEHPLTLHAVFKDGHEVVGESQIADYKGMIDHVY
                     VTNTYNEETPTASRKVVDAILESDMIVLGPGSLFTSILPNLVIPEIKQALLETRAEVA
                     YVCNIMTQRGETEHFTDADHVEVLKRHLGQDAIDTVLVNIEKVPESYMENNHFDEYLV
                     QVEHDFSGLRKHARRVISSNFLKLEKGGAFHHGDFVVEELMNLVGTHL"
     gene            9260..10171
                     /gene="whiA"
                     /locus_tag="VBE22_000154"
     CDS             9260..10171
                     /gene="whiA"
                     /locus_tag="VBE22_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEO7201635.1"
                     /translation="MSFTVKVKEELLGHKSENKMELSAIIKMSGSLGLANHGLNLSIT
                     TENAKIARHIYSMLEEHYHLQPEIKYHQKTNLRKNRVYTVFIEEKVDVILADLKLADA
                     FFGIETGIEHSILDNDENGRAYLRGAFLSTGTVREPDSGKYQLEIFSVYLDHAQDLAN
                     LMKKFMLDAKVIEHKHGAVTYLQKAEDIMDFLIVIDAMEARDAFEEIKMIRETRNDIN
                     RANNVETANIARTITASMKTINNIIKIMDTIGFDALPSDLRQVAQVRVAHPDYSIQQI
                     ADSLETPLSKSGVNHRLRKINKIADEL"
     gene            10186..11583
                     /locus_tag="VBE22_000155"
     CDS             10186..11583
                     /locus_tag="VBE22_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEO7201636.1"
                     /translation="MACTTILVGKKASYDGSTMIARTEDSVNGDFTPKKLKVMTSKDQ
                     PRHYKSVLSNFEVDLPDNPLPYTSVPDALGKDGIWGEAGINSKNVAMSATETITTNSR
                     VLGADPLVSDGIGEEDILTLVLPYIQSAREGVERLGAILEKYGTYESNGIAFSDTEEI
                     WWLETIGGHHWIARRVPDDVYVTNPNQLGIDHFEFNNCDDYMCSSDLKEFIEQYHLDL
                     TYSNEHFNPRYAFGSQRDKDRHYNTPRSWAMQRFLNPEIEQDPRSLFIPWCQKPYRKI
                     TVEDIKYVLSDHYQDSVYDPYGPEGDAVSRRAFRSVGINRTSQTSILQLRPNKSLETT
                     GVQWLSYGSMPFATMVPLFTQVETVPDYFSNTTKDASTDNFYWTNRLIAALADPHFYQ
                     HEADIESYIERTMAQGHAHINGIDREVAENKEIDFQQKNQEMSDYIQKESQELLNRIL
                     FDASNLMTNRFSMGD"
     gene            11725..13251
                     /locus_tag="VBE22_000156"
     CDS             11725..13251
                     /locus_tag="VBE22_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEO7201637.1"
                     /translation="MRKKFLLLMSFVAMFAAWQLVQVKQVWADSKLKVVTTFYPVYEF
                     TKNVVGDKADVSMLIKAGTEPHDFEPSTKNIAAIQDSNAFVYMDDNMETWAPKVAKSV
                     KSKKVTTIKGTGDMLLTKGVEEEGEDHEHEGHGHEGHHHELDPHVWLSPERAISVVEN
                     IRNKFVKAYPKDAASFNKNADAYIAKLKDLDKEYKNGLSNAKQKSFVTQHAAFGYMAL
                     DYGLNQVPIAGLTPDAEPSSKRLGELAKYIKKYNINYIYFEENASNKVAKTLADEVGV
                     KTAVLSPLEGLSKKEMAAGEDYFSVMRRNLKVLKKTTDVAGKEVAPEEDKTKTVETGY
                     FKTKDVKDRKLTDYSGNWQSVYPLLQDGTLDPVWDYKAKSKKDMTAAEYKKYYTAGYK
                     TDVESIKIDGKKHQMTFVRNGKSQTFTYKYAGYKILTYKKGNRGVRYLFEAKEKDAGQ
                     FKYIQFSDHGIKPNKAEHFHIFWGSESQEKLFEEMENWPTYFPAKMSGREVAQDLMSH
                     "
     gene            complement(13364..13624)
                     /locus_tag="VBE22_000157"
     CDS             complement(13364..13624)
                     /locus_tag="VBE22_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEO7201638.1"
                     /translation="MKKDIHPDYRPVVFLDTTTGYKFLSGSTKSTKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAQ"
     gene            complement(13733..14668)
                     /locus_tag="VBE22_000158"
     CDS             complement(13733..14668)
                     /locus_tag="VBE22_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEO7201639.1"
                     /translation="MIIFQQILDKIKEYDTIIIHRHMRPDPDALGSQIGLRDIIRHNF
                     PKKKVLATGFDEPTLTWIAKMDQVTDQDYQGALVIVTDTANTPRIDDERYKKGDFLIK
                     IDHHPNDEIYGDLSYVDTNASSASEIVTDFALSCDLLLSTSAARVLYNGIVGDTGRFL
                     YPATTSKTLKIASKLREFDFDFSAMARQMDSFPFKIAKLQGFIFEQLKIDKNGAACVT
                     LTQEDLKRFDVTDAETAAIVGVPGKIDIVESWAIFVEQSDGHYRVRLRSKSHIINEIA
                     KRHNGGGHPLASGANSYSLEENQAIYQEIQEVLSL"
     gene            14934..15956
                     /locus_tag="VBE22_000159"
     CDS             14934..15956
                     /locus_tag="VBE22_000159"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEO7201640.1"
                     /translation="MTQAVLKELAKAELHCHLDGSLSLPAIRKLANMADIILPSSDKE
                     LRKYVIAPAQTESLVDYLKTFEFIRPLLQTKEALRFAAYDVARQAALENVIYIEIRFA
                     PELSMDKGLTASDTVLAVLEGLADAQKEFNIVARALVCGMRQSSHKTTKDIIKHIVDL
                     APKGLVGFDFAGDEFSYPTDSLVDLIQEVKRSGYPMTLHAGECGCAKHIADSLNLGIK
                     RMGHVTALTGQRDLIKRFVEEDAVAEMCLTSNLQTKAASSIQSFPYQELYDAGGKITI
                     NTDNRTVSDTNLTKEYSLFVTYFGTKIEDFLVFNQNAVKASFTSDSEKDTLLHKLQEN
                     YDSYLK"
     gene            16015..16458
                     /locus_tag="VBE22_000160"
     CDS             16015..16458
                     /locus_tag="VBE22_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEO7201641.1"
                     /translation="MALAKIVYASMTGNTEEIADIVADKLRDLGLDVEVEECTMVDAA
                     DFEDADIAIVATYTYGDGDLPDEIVDFYEDLAEVDLSGKVYGVVGSGDTFYDYFCKSV
                     DEFEAQFALTGAQKGADCVKVDLAAEDEDIENLEAFAEEIASKLN"
     gene            16535..16810
                     /locus_tag="VBE22_000161"
     CDS             16535..16810
                     /locus_tag="VBE22_000161"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEO7201642.1"
                     /translation="MELEEIRQEIDEIDQQLVSLLETRMGLILEVIAFKKKHRLPVLD
                     NNRENEVLNNVLKKVQNHQFDDVIRATFKDIMTESRVYQKENIVDGD"
     gene            16803..17999
                     /locus_tag="VBE22_000162"
     CDS             16803..17999
                     /locus_tag="VBE22_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEO7201643.1"
                     /translation="MIKRFDNYAQYVLVLGFSLLIGGVVGAITAVFGRVLLFLTAFRS
                     DYIAYLLPFLSIVGLFIVFVYQKFGGKSVKGMGLVFEVGHGNEETIPKRLVPLVILTT
                     WLTHLFGGSAGREGVAVQIGATVSHYFQKYCRLQNASQLFLVMGMAAGFAGLFQTPLA
                     ATFFAIEVLVVGRLMVSYILPSLIAALTANFVSHSLGLEKFSHSIATSMALTPDIILK
                     LLVLGLCFGLCGNLFAYLLAKAKLIASSRLLNPYKRIFTLGLLATFLLFIFHFGRYSG
                     LGTNLIEASFTNKNLYDYDWLLKLCLTVITLAAGYQGGEVTPLFAIGASLGVIIAPIL
                     GLPVILVAALGYTSVFGSATNTLLGPILIGGEVFGFANTPYFVIVCLVAYSISHAHTI
                     YGAQSR"
     gene            18583..18930
                     /gene="rplS"
                     /locus_tag="VBE22_000163"
     CDS             18583..18930
                     /gene="rplS"
                     /locus_tag="VBE22_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEO7201644.1"
                     /translation="MNPLIQSLTEGQLRSDIPEFRAGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            19041..19112
                     /locus_tag="VBE22_000164"
     tRNA            19041..19112
                     /locus_tag="VBE22_000164"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19074..19076,aa:Arg,seq:cct)
     gene            complement(19155..20234)
                     /locus_tag="VBE22_000165"
     CDS             complement(19155..20234)
                     /locus_tag="VBE22_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7201645.1"
                     /translation="MWIEELANGKFKYIERYTDPLTNKYKKVSVTLDKNSSQAQKKAG
                     LILQEKIEDRLAIRNHSEMTYGELKKEYLKQWIPTVKDSTKRGYLVSDSHIATVLPDD
                     TIINKLTKRDIRLIIDKLLKHNSYHVTHKCRKRLHAIFSYAIQMDYMTSNPTENVLVP
                     KPKDDYKPEKVLYLTSNEVYDLCNRMIDNDEQTLADIVLFMFLTGVRYGELACLTYDK
                     IDFENKEILINATYDFNTREITTTKTKKSTRKISVSDNILDIVNRQKKTSSFVFPNSN
                     GVPILNAYINKRLKIYGDYHTHLFRHSHISFLAEKGIPLNAIMDRVGHSDPKTTLSIY
                     SHTTVNMKEIINKQTAPFVPLLKSE"
     gene            complement(20358..21251)
                     /locus_tag="VBE22_000166"
     CDS             complement(20358..21251)
                     /locus_tag="VBE22_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HEO7201646.1"
                     /translation="MENEVLHATHEGKLVLDNTTIDVAVLNNGQRIITQAGVFKALDR
                     PSRGNTRVIGIPTFMDAKNLQPLISKDLRGVINKIEYRDLNGRIQLGFDANILPLVSD
                     LYLKAREIGAIKSEAQMRTAQKAEMLVRSLAKVGVTALVDEATGYQYERERFELQKIF
                     KAYISEELLKWQKMFPDDFYMEIFRLNGWSYDVQSIKRRPGVVGKWTNELIYKRLPDG
                     VLEELKRRTPKTKKGQYKARFFQTLTPDIGHPDLNAQIYKVLGIMRISSNWKDFKEKF
                     NVMTSREDGIIELDLEFDEDK"
     gene            complement(21423..21980)
                     /locus_tag="VBE22_000167"
     CDS             complement(21423..21980)
                     /locus_tag="VBE22_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEO7201647.1"
                     /translation="MLEKVERLISEINRIHLVYSQDYFETGKVEKINLKHTFSKVPVK
                     AILDYRLNLHESINDYLMKADVKDIPYVYRVKTSESILDKIERFSKRQDGYPVNSILN
                     DIFGARIILSSEDISQVMEQLDEWKDKFDLKNWYLRDKDNYTGIHVYFKNKSNHYYPW
                     ELQIWDEKDVDQNIESHKLFKRHFV"
     gene            complement(21982..22779)
                     /locus_tag="VBE22_000168"
     CDS             complement(21982..22779)
                     /locus_tag="VBE22_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7201648.1"
                     /translation="MHLGKYIKKYRDTNNLSMAEFAKESGISKAYVSILEKNRDPRNG
                     KEIIPSIPIIKKVSDTIGISFDDLLNSLDENQIVALNETKTEKNLTSSTLQKITSTSS
                     QLEQPRQEKVLSFANEQLEEQNNVISMFDRKVEETENYITDYVEGLVAAGLGAYQEDN
                     LHMEVKLRADDVPDKYDTIAKVAGNSMEPLIQDNDLLFIKVSSQVDMNDIGIFQVNGK
                     NFVKKLKRDYDGAWYLQSLNKSYEEIYLSENDNIRTIGEVVDIYRES"
     gene            23135..23278
                     /locus_tag="VBE22_000169"
     CDS             23135..23278
                     /locus_tag="VBE22_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201649.1"
                     /translation="MPEDLIKQLEAGSKFLAKTCLHSKIIITVDGIQLVETKEFHPRN
                     ELL"
     gene            complement(23398..23580)
                     /locus_tag="VBE22_000170"
     CDS             complement(23398..23580)
                     /locus_tag="VBE22_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HEO7201650.1"
                     /translation="MYFVIRKSSNNQFYFVIKADNHEVVATSETYYFKDSAIRTIKSI
                     KKGISTDSNVVDMTSD"
     gene            23638..23796
                     /locus_tag="VBE22_000171"
     CDS             23638..23796
                     /locus_tag="VBE22_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201651.1"
                     /translation="MNWKKLMFGDLEHTFTSRNGKEKTSIEFEGGVLPALLVLGGITW
                     LIAWFITK"
     gene            complement(23943..24638)
                     /locus_tag="VBE22_000172"
     CDS             complement(23943..24638)
                     /locus_tag="VBE22_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201652.1"
                     /translation="MSKKSCFVVSAIGEESSEIRNHSDSVLNYIIKPALIEKYQVTRA
                     DELYHSDRIDDKIFDALSTADLVIVDITGNNPNVFLELGFRKALNLPTIFLRQKTDED
                     IPFDIRTINTIHYDLKNSESKVVLDSVQETIRRIQKTEENIDFSIIHEPNDQSASVQD
                     IIQLKTSINNIYDAIENLSDKIENNSTQKRPMTQEDLIMMAFQEPEKLEKIFELQTKY
                     PNAFNSPSNALND"
     gene            24693..24902
                     /locus_tag="VBE22_000173"
     CDS             24693..24902
                     /locus_tag="VBE22_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201653.1"
                     /translation="MRPKKYPYSQKNYPAPKLHNIIETDNHFLIDDKRIHYVIEDSVK
                     TKTLGDGYVEVTLSIIAKSFTKSQG"
     gene            25134..25325
                     /locus_tag="VBE22_000174"
     CDS             25134..25325
                     /locus_tag="VBE22_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7201654.1"
                     /translation="MQQFNLKQLREKKGFTQNELADKANVSRSLVVGLETGSYSETST
                     ASLKKLAKALDVKIKDLFF"
     gene            25392..26312
                     /locus_tag="VBE22_000175"
     CDS             25392..26312
                     /locus_tag="VBE22_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEO7201655.1"
                     /translation="MNELTLSDNLAQIELDLRQENEQIGKSIWKIGCMLKHVKENDLT
                     HGQFMDWYKNLGYNKNFVSKAITIADKLSNFPTLGNIGTEALYLIATLPDEQKQEQIE
                     RIESGDNPTVRELQEIKRENNRLKSENARLEQQKENLAEQALSAKIVEKEVIKEVIPD
                     DYESTKQLNQTLLGKNKELSKMVDDALQHEEYLKSQLKEFYAKRDEVNHKSAKYDELT
                     EAIKQSEGKLNSYQKKIASYKNITELLKKGDLLLLEMSGLIYADETHYIQRDGLIKQE
                     FDSLVDRGLKLFNDLDMKRKNTEILEGEVL"
     gene            26309..26764
                     /locus_tag="VBE22_000176"
     CDS             26309..26764
                     /locus_tag="VBE22_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEO7201656.1"
                     /translation="MNELTTTQQLIEMSKMQTVTLEKVDNLEKGLLQAQNDIQEIMDT
                     SYLHPGIINMITKKRRKRVIDCMGGKSAKAYKTFKVDEEGRKHRFSSEVFREMELDFK
                     AEFDLNSYAELSKSKKEEALEYIAMWEPCTNTKRKINLLNKQTELELIG"
     gene            26797..26955
                     /locus_tag="VBE22_000177"
     CDS             26797..26955
                     /locus_tag="VBE22_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201657.1"
                     /translation="MEEDIKQLITENILEFLGSDYGKGFLTGIKIAVEIIAKQIPTPS
                     EEDIGHDS"
     gene            complement(27191..27499)
                     /locus_tag="VBE22_000178"
     CDS             complement(27191..27499)
                     /locus_tag="VBE22_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201658.1"
                     /translation="MNDVLKTNLIADVAIFSEKSNCKLNVITASGIFTGTLLPENPDK
                     AKYAHVLEFLEYRKENKDDNERFMLLVDATLSTSKESTLNLPFVVLFIDQIIGVSSVQ
                     "
     gene            27605..27835
                     /locus_tag="VBE22_000179"
     CDS             27605..27835
                     /locus_tag="VBE22_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201659.1"
                     /translation="MLAKLKSGIEVPYEELWLNDNDLSEFIGKSFDQTQRLLRKMYKD
                     RNYRKYIDKVGGRSTKVKKFEEWRKLQNEKII"
     gene            27819..27965
                     /locus_tag="VBE22_000180"
     CDS             27819..27965
                     /locus_tag="VBE22_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201660.1"
                     /translation="MKKLFNFIFTKPKKEEPKPKRTIETHGWEVGAKAYDDFHNYMKG
                     LKHG"
     gene            complement(28243..28335)
                     /locus_tag="VBE22_000181"
     CDS             complement(28243..28335)
                     /locus_tag="VBE22_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019781509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEO7201661.1"
                     /translation="MCEIFFTTIIAPLLVGIVLLLVQKWLDNDD"
     gene            28900..29070
                     /locus_tag="VBE22_000182"
     CDS             28900..29070
                     /locus_tag="VBE22_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201662.1"
                     /translation="MAKMGRPTSNPRDKYIGVRASKNEVEMLDFCTEKTGKSKTDILM
                     EGLEKVYNELKG"
     gene            29247..29687
                     /locus_tag="VBE22_000183"
     CDS             29247..29687
                     /locus_tag="VBE22_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201663.1"
                     /translation="MKTYTLTEEELNELVAERMKQAKEKRTPQGLFKDVGFDDELIPI
                     NNKYPKVLKKLNRERAYKPEKHVFNQTPKVFGVDNEISYSKITTHDVHNHIRLLVLNV
                     FGKSQNKEVLPEEYDQAIELYNQLKEWFVSSYDKRLEGLVLEDD"
     gene            29803..30705
                     /locus_tag="VBE22_000184"
     CDS             29803..30705
                     /locus_tag="VBE22_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HEO7201664.1"
                     /translation="MAQKSKTKIYYWLKFDKHFFENLFIKRLLRNFPGGSDMIVIYIR
                     LMLEAIENDCIIDYEGTFDNYAEELALRLETSEEQINMALAYFVKCGVIQVDDGGNTH
                     YPQAKALLGQETNWNRYKKKQAELEKFQPLSNKVPTEIEIEIDKEKDKEKDKEKEYIV
                     EQSPTEYLFPNWLEEKYVEQVKKGNPKNFDCRIPIAYLNQKTNSNYKFVDSNINLVKT
                     RLKDGYTLDDFKTVIDKKCSEWGNSDMAKYLRPSTLFNASKFESYLNQPEASNGDYYQ
                     KQQGQRFSQAELDELKKPDPKYGF"
     gene            30715..31557
                     /locus_tag="VBE22_000185"
     CDS             30715..31557
                     /locus_tag="VBE22_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7201665.1"
                     /translation="MAFGLMTRENMLENGIIRDTGKTCEKHEMPIYARKMPNHGNRET
                     EFCWQCTTEYIQSKSNAVDIAYNNQSLLAKGYKVFYKESVLSKEIASATLKNYKEHSA
                     VDTKALNYAKRITRDYVKGMEGNSLLQGPPGVGKSHLSMSIAKNINEMFKSYNQSKSV
                     IFVSVPLLSGLVKDTFDYDDKKNSKYSQERMSKLLINCDYLILDDLGKESTTGNTIKS
                     ASGWTYTFLFNILDNRTNTIINTNFSRAELMKIYDAAFVDRIIKGAKNNIFKYPDNAE
                     SKRF"
     gene            31557..31703
                     /locus_tag="VBE22_000186"
     CDS             31557..31703
                     /locus_tag="VBE22_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201666.1"
                     /translation="MELTLTTFFSLSEEHAAKIMALDEPSRNKKIEEYRQLRLRRGRI
                     DFGK"
     gene            31693..31968
                     /locus_tag="VBE22_000187"
     CDS             31693..31968
                     /locus_tag="VBE22_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201667.1"
                     /translation="MENRPDLKLVAELENRIKDLKIENEILKSKNIDLSEDVKHLKYK
                     CFEKDSSIVDIMVNNNDLRKENNELREMFDFFKDRLEKFVGSSHGRN"
     gene            31955..32209
                     /locus_tag="VBE22_000188"
     CDS             31955..32209
                     /locus_tag="VBE22_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201668.1"
                     /translation="MVEIRINGELVTFDGNFRDAFIFTIDYLRDSEEPTLRQTYNEFK
                     DYTDEDLMEYIETEFDVKPELIVNRRLDSKWAFKSHILED"
     gene            32212..32373
                     /locus_tag="VBE22_000189"
     CDS             32212..32373
                     /locus_tag="VBE22_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201669.1"
                     /translation="MSEELYESTRYWQSRYSDLMSDYLKEAEENIELKKQLKRLKAEN
                     WQLKHRKRK"
     gene            32374..32856
                     /locus_tag="VBE22_000190"
     CDS             32374..32856
                     /locus_tag="VBE22_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HEO7201670.1"
                     /translation="MAYLYELQGIYAQLSAMDLDDETFQDTLDSIDFQSDLENNIEYF
                     VKMLKNTQADIEMYKNEKETFYKKQKQAEAKAEKYKDTIRLAMDLSQKKKVDAGMFKV
                     SLRKSKKVEVLDETKIPFEYMQEKVEYKPKKDEISKVLKSGIDISGVQLIETESLQVK
                     "
     gene            32857..33531
                     /locus_tag="VBE22_000191"
     CDS             32857..33531
                     /locus_tag="VBE22_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HEO7201671.1"
                     /translation="MSMTFAELQTKMQITKTTKQGVKYTFRNAEDIFTHFKTLNSGWE
                     LTVSDELVELIGRIFIKATATARLGDEQHQATRYAELDSVPVLNTKDYKTGEPKQIQQ
                     MQVPQWTGAVSSYAGKYALQGLFGIGEEDVDAIVTEDTQRKEQKTSRPTTSKTPKISN
                     IQVETYKSDLNDIAKATNQNVEELTKWLTDTLKVGTLENLHTEHIVSADELINKLKKK
                     AGLKND"
     gene            33524..33940
                     /gene="ssb"
                     /locus_tag="VBE22_000192"
     CDS             33524..33940
                     /gene="ssb"
                     /locus_tag="VBE22_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7201672.1"
                     /translation="MINNIVLVGRMTKDAELRHTPSQVAVATFTLAVNRRFKEQNGER
                     ETDFINCVIWRQSAENLANWAKKGTLIGITGHIQTRNYENQQGQRIYVTEVVAENFQL
                     LESRNSQQQTNQSGNSSNSHFGNANKMDISDDDLPF"
     gene            33943..34143
                     /locus_tag="VBE22_000193"
     CDS             33943..34143
                     /locus_tag="VBE22_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201673.1"
                     /translation="MTKPRKQRIYAIYDDDKFVDVGTKEELSARLGIKKATIEQYMTK
                     SYQARPSSKRIAIFVGVEEIEF"
     gene            34133..34621
                     /locus_tag="VBE22_000194"
     CDS             34133..34621
                     /locus_tag="VBE22_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEO7201674.1"
                     /translation="MNFKTEFEIPVEPKPQTRPKFSKWGTYEDPKMKKWRKQVTGWIE
                     KNYNGPFFDNCVKVDVTFYMRAPQTLIKEPTARSKSKTVQIYQKFINELIWHVKKPDI
                     DNLVKAVFDSISDAGYDKIQKSGIVWSDDNIVCDLRATKKYSPNPRIKVKIEEIDERT
                     NG"
     gene            34602..34946
                     /locus_tag="VBE22_000195"
     CDS             34602..34946
                     /locus_tag="VBE22_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201675.1"
                     /translation="MNELTDKFYSIFDSSILRRVKELNLDDKTSERLRLNISNNKRRN
                     ILPRPYVIEAFKDYFDKDTYVQLYLKSYREYHNPNSHETDIFIKLNKKHRDTKLDHYK
                     KVKRLMYAAMTF"
     gene            34958..35254
                     /locus_tag="VBE22_000196"
     CDS             34958..35254
                     /locus_tag="VBE22_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HEO7201676.1"
                     /translation="MADKINAESMQAAYNENYQMFLAKNADYGNSFEKSLNDFGFIAG
                     VVRIGDKYNRLYNLISSDKNVSESLSDTLNDMANYCTMLSIWLEKTENANDTRS"
     gene            35238..35477
                     /locus_tag="VBE22_000197"
     CDS             35238..35477
                     /locus_tag="VBE22_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201677.1"
                     /translation="MTHVVRVYDHIGGRVLPITRRVRRMTKYIEIKDDWKKAVDHLND
                     FIEKYSYKKIVVVGYQVVRNPETNGTCTHVLVKVD"
     gene            35508..35672
                     /locus_tag="VBE22_000198"
     CDS             35508..35672
                     /locus_tag="VBE22_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201678.1"
                     /translation="MTKEEVIAFLTEQRDLRLVAYEWGKDNLSVFARWQLEQANMYLD
                     IIEWIEEVTE"
     gene            35669..35938
                     /locus_tag="VBE22_000199"
     CDS             35669..35938
                     /locus_tag="VBE22_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201679.1"
                     /translation="MTEQQMIDCLLYELAKKDKLNIRRNNIITFLSIVLMAISILNVA
                     LQDHYKLQITGLRTQLSRTQKQLKRASEQNQRQTKRIAELTGNGG"
     gene            35942..36085
                     /locus_tag="VBE22_000200"
     CDS             35942..36085
                     /locus_tag="VBE22_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201680.1"
                     /translation="MIDEILKRLNKEFDNDLDNYEQERYAGYMDAIGVAIEIVEEVKR
                     GRK"
     gene            36082..36594
                     /locus_tag="VBE22_000201"
     CDS             36082..36594
                     /locus_tag="VBE22_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEO7201681.1"
                     /translation="MNIEEAKKLIDKQSIGKGGVGDIPVVKTHIVKVLLDQLDKPKLE
                     VPQMIADVIESFDEDVNYLHEHMSYQSDEVREWLTHNEREFYEAWLAYPNITVEKENL
                     YTVEIPNPNERQLSFVLMRQLSGNVSIKVMHRDNLDLLKIDNNLQLTESEIRKDFDWA
                     WQFRKDVENE"
     gene            36615..36914
                     /locus_tag="VBE22_000202"
     CDS             36615..36914
                     /locus_tag="VBE22_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201682.1"
                     /translation="MNYDKNKNDAKKNFIIALVLLPFGLVLSGFVIKYGWNNILSTID
                     GVPSINLPQAVGINVLISPFASKKNTDEDFATVIARAFISPLVVLLLLWIVTLFM"
     gene            37102..37296
                     /locus_tag="VBE22_000203"
     CDS             37102..37296
                     /locus_tag="VBE22_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201683.1"
                     /translation="MTYLKKLFEAVKFLNSMEKQNKKIMITFHNKKVHFYELEEITES
                     SSGTVEAKAKMLSLWGKIKL"
     gene            37293..37559
                     /locus_tag="VBE22_000204"
     CDS             37293..37559
                     /locus_tag="VBE22_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201684.1"
                     /translation="MKEKTIFISKKYANDFNNDKYNLSSGYYFRSGEKHDIAIVKYGE
                     KDYLKNTDLAYVVCDKIVDADSISFVYHGEYETWHFKLLNTEAN"
     gene            37950..38384
                     /locus_tag="VBE22_000205"
     CDS             37950..38384
                     /locus_tag="VBE22_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEO7201685.1"
                     /translation="MTFFPEINIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQSHGTPSKPVERLALNRVSAEQELEAIEQSVSMILEPEKRRILYDKYLSPYKNAD
                     KVIYTELCMSESFYYDTLDAALLAFAELYREGSLIVEQGVFD"
     gene            38550..38628
                     /locus_tag="VBE22_000206"
     tRNA            38550..38628
                     /locus_tag="VBE22_000206"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            39047..39937
                     /locus_tag="VBE22_000207"
     CDS             39047..39937
                     /locus_tag="VBE22_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEO7201686.1"
                     /translation="MLKLDEKKIRKGKPFGLPYQGSKKKISKKIVEIIKQNFGTDKKI
                     YDIFGGGGAITAECMLNNLEVHYNDLNNDVTDMFKRVITQDREWLKTLIISREEFLKI
                     KTKTNKTVDDNIKLLVNSFGNNSISFLYNIELSDIKYKLAVEIINKHDVFSGYKQTDT
                     YKETCLLCEEEKLGKIGNLQRLQQLEQLQRLQQLEQLERLEATNLDYKAFSNVDGAIF
                     YLDPPYEMTSQHSYIGDTFDSQLFYDWAYEMSKENIVIVSSYEISDDRFKCVYEFKTA
                     RSTMQGGSACGKTEKLFMVV"
     gene            complement(39990..40373)
                     /locus_tag="VBE22_000208"
     CDS             complement(39990..40373)
                     /locus_tag="VBE22_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEO7201687.1"
                     /translation="MLIYPAVFTHDNETNSIGVRFPDVPEAITFGNDLKHAYEMAVEV
                     LGFALEDYTDYPKASSVSDLKEQYPDSDIALIGIDMIAYMKKYHSKKVRKNVTIPEWL
                     NNAAEDKNLNFSQVLTEALELKLQA"
     gene            complement(40417..40605)
                     /locus_tag="VBE22_000209"
     CDS             complement(40417..40605)
                     /locus_tag="VBE22_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEO7201688.1"
                     /translation="MPRTAREIIKLLKKNGFVKVSQNGSHAKYKNFETGNITIVPMHK
                     GDMPKGTEDDILKKAGLK"
     gene            40714..41211
                     /locus_tag="VBE22_000210"
     CDS             40714..41211
                     /locus_tag="VBE22_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEO7201689.1"
                     /translation="MGKLTLKQQKFIDEYIICGNATDAAIKAGYSKKTAGQIGEQNLK
                     KLEIKQAIQSRMKVLEKSSIATADEVLRVFTQILRQELTEEVTELNQITGEFVTIEKQ
                     PSIAEVIKAGSELMKRYPTNLELKKINLEIEKLKSQIGGDEGQDEKIANFLNMVKGAI
                     SDGVE"
     gene            41198..42439
                     /locus_tag="VBE22_000211"
     CDS             41198..42439
                     /locus_tag="VBE22_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEO7201690.1"
                     /translation="MELSKLYTRKQLQVLDYIWNNDWFICGLHGAKRAGKTVVNNDTF
                     VTELYRVRKIADDLKIDEPIYILAGTSSTSIQNNVLQELYNKYGFEPKYDKHGSFTFC
                     GVKVVQVYTGSISGLKRARGFTSFGAYVNEASLANETVFKEIISRCSGEGARIVWDSN
                     PDNPNHWLKTDYIDSDDDMIIDFSFKLDDNTFLSKRYIESIKSATPSGKFYDRDILGK
                     WTVAEGAIYSDYDKNVHEVEELPKMTRFFAGVDWGYDHYGSIVIIGEDNNGNYYLVDG
                     IAEQYKEIDWWVTRAKEFIQIYGNVTFWADSARPEHVARFKRERIKARNGRKEVVAGI
                     EYIAKSFKDKRLFIKRGCIPRFFDEIYQYKWKPNSTKDEPQKEYDDVLDAIRYALYSQ
                     HKEDSISKTNNFNVLYQGLKN"
     gene            42452..43945
                     /locus_tag="VBE22_000212"
     CDS             42452..43945
                     /locus_tag="VBE22_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO7201691.1"
                     /translation="MAHIEDFIDSTGEHKLLELRFHRESRMRYQISDISALFDDNYKI
                     LLEYLKHHENIQKPRIQELLDYAEGNNHEVSKSGRRQDEDMADVRAIHNYGKYISTFK
                     QGYLVGNPIRVEYDDEVNNELLKELAKKNNFHQLNRQLIKDLSKVGRAYELAYRSADD
                     KTKVIRLDPRETFIIYKNDVDKDSLIGVRYYNKSQIDKTDKTVEVYTSSEVIFFEFNG
                     ELTETDRQPHAFGAVPITEYLNTDDGLGDYETELSLIDLYDSAQSDTANYMQDLSDAI
                     LAIIGRVSFPEYVDTQEKAITYLRAMRKARLLNLEPPVDSEGREGSVDAKYLYKKYDV
                     QGTEAYKNRIAEDIHRFTNTPDMTDNKFAGNQSGEALKWKVFGLDQERVDLQALFEQS
                     LKRRYKLIAHVSEFLSEIKDFDISKLKIIFTPNLPKSDQEKINDFKALGGELSNKTKM
                     FVTGIVDDPDEEEDKIMQESQSGSLLAQKLEAQTRMSDKELAHGHAH"
     gene            43929..44900
                     /locus_tag="VBE22_000213"
     CDS             43929..44900
                     /locus_tag="VBE22_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEO7201692.1"
                     /translation="MDMHTKEGKSYWRKRVKKEMEAKDKKDVSLGKSMKQIHDYHFRE
                     IEKEIESFYQRYADKEAIDLKLAKKAVSDVDINAYQKKAKELVARANEMRKEGIKVTK
                     ANFTHQENIDMAVYNLKMKVNALELLQLNIDLAMQNLSEDEYKATKRFLEDGFEEELK
                     FQSGLLGTSVSSQNDIKNLAKATINANFKGATWSENIWQRQDDLRKIVKEEVYKAVTK
                     GDNAIKLSNKLKKEFEVSDSYARRLAITEHARVQMEVSKISIEDNGFNGFEILPEPSA
                     CSICKGIASDGPYPMEKWDTGNTAPPFHPHCRCAVVGEDIEHKKGKK"
     gene            44897..45100
                     /locus_tag="VBE22_000214"
     CDS             44897..45100
                     /locus_tag="VBE22_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201693.1"
                     /translation="MNKRIKKKRKLEYYIASLVAENVMFSKELIKQHERIEQLEKIVE
                     HNAQATNNELSRIKKHSKKKWKK"
     gene            45211..45789
                     /locus_tag="VBE22_000215"
     CDS             45211..45789
                     /locus_tag="VBE22_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEO7201694.1"
                     /translation="MADLVNNGVVDEVTQEEVETQEEVKAEATSEKTFTQAEVTEMIQ
                     QNVNRAVAKAHKDAQEQFKAEQDEAKKLAKMNSEEKANYETQKLLEELQQLKDDKTRN
                     ELTAVARKMFSEADINVDDDVLSLAVTLDAEQTKANVTKLASAFAKAIADDRKSLARQ
                     ATPSIGSGSITTQSNFGASLAERAGKVNTKLF"
     gene            45799..46182
                     /locus_tag="VBE22_000216"
     CDS             45799..46182
                     /locus_tag="VBE22_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HEO7201695.1"
                     /translation="MNKRTVKTSKEILHNLPYEAISVTLDANAFGKGLASAGTVLAGD
                     GASVFKDRTKKVKPVRNAEVSGADHIDGILLTDVDLSKGDATGALVYRGTVNSDKLVD
                     QELAKDTSTLATKLPHIVFVKGGTK"
     gene            46182..47234
                     /locus_tag="VBE22_000217"
     CDS             46182..47234
                     /locus_tag="VBE22_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HEO7201696.1"
                     /translation="MALIYDVVTSANIKGFYDKQQANVDLTLGEKAFPSKQQLGLKLS
                     FIKGAAGKPVSIKAAAFDTKVPLRDRIAVVLLDEEMPYFKEGMLVKEADRQQLNVLAQ
                     TKNQELIDTVLSTIFNDETTLIAGAKARLEAMRMEVLSSGKIHINSNGVMKDIDYGLT
                     GTQTTKSEQKWSEKDTANPLADIEKAIETVTERGHVPEAIVLNSKTFGYIKNAKATVK
                     AIKPLAPEGSIVTKAELKSYLSEELGLNILLKDGVFVNDAGESKKYFPDGVVTLVPNG
                     NLGYTVFGTTPEQSDLMGGQATDAQVSLVETGIAVTTTKTTDPVNVQTKVSMIALPSF
                     ERLDEVQIVTSSEVSL"
     gene            47247..47501
                     /locus_tag="VBE22_000218"
     CDS             47247..47501
                     /locus_tag="VBE22_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201697.1"
                     /translation="MALVIEAFRDKETGFIYKVGEEYNGARVEFLTGKGVLKATDTQS
                     TNFSNLKVDELKRGLDELGVNYDSKAKKAELLELLESHTD"
     gene            47517..47870
                     /locus_tag="VBE22_000219"
     CDS             47517..47870
                     /locus_tag="VBE22_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO7201698.1"
                     /translation="MEKINTQTIINNVKLDLDINDTLQDKLLEMLLKRITDHFSAEYG
                     TNEIDSAFSFVLEDCLIARYNRRGAERAKSESVEGRAITYYDFLNEFEPYDLMIKSKL
                     NISNQKSKKGGLYFL"
     gene            47867..48166
                     /locus_tag="VBE22_000220"
     CDS             47867..48166
                     /locus_tag="VBE22_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201699.1"
                     /translation="MRYNDRVTLLIKANGEPRYDPTLGKRVGGEVKKDVVPCNISEQG
                     IELSNLLDEKLDLDRRIVRLRHSVKKVDRVLIKDKAYRVVTSRNKALYVEEIVND"
     gene            48159..48539
                     /locus_tag="VBE22_000221"
     CDS             48159..48539
                     /locus_tag="VBE22_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017827930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO7201700.1"
                     /translation="MINLTLEGEHELLSALKKEVTFENKRKAVRKNGRKLHKKAVKNA
                     NFNGHYKKRKGEEYVFVKPTGATRRSIKLEFSNQSTVAKVKAGTNYSGYLETGTRLME
                     EQPFMKPALDSVIDNFIKDLARVE"
     gene            48536..48925
                     /locus_tag="VBE22_000222"
     CDS             48536..48925
                     /locus_tag="VBE22_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HEO7201701.1"
                     /translation="MMKQPDQQLFDEIFKRLTDIGLTVYDFLPPLGTKYPFVVMGDTH
                     IIPMATKTQLIGKCSTTINVWGDGTNRKVVSDMIAQIMLEVSKISQIDGNKWAMVMNE
                     SDTQILKDNSTNENLYHGILNIYFNFV"
     gene            48934..49467
                     /locus_tag="VBE22_000223"
     CDS             48934..49467
                     /locus_tag="VBE22_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEO7201702.1"
                     /translation="MVTSSPIYGKDNILMFRVLGDKAAAAKLSFQTEHKWKYSRKTDA
                     KITKDGAINSDKGLEVTLEIKGVASRDELNTTLKNAVLEGKQIEVWDIDLNSNNDSDG
                     KYDAEYAIGRLGSWEVPSNVEEFSEISTEMAIDGKPVKGKATLTKEQIKAIQYVFKDV
                     TKIDESSVSSPGSVHSR"
     gene            49540..49899
                     /locus_tag="VBE22_000224"
     CDS             49540..49899
                     /locus_tag="VBE22_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201703.1"
                     /translation="MKEITISGKNYPLNFGFDFIREMDKRFFVDNNGFKFGTGVQTAV
                     MQLAAKNPLILEDIILSATHTEYSIPKKEDIEKWAIEQAENGKLGEVLDDFLTILKKA
                     PLLKTQVSPILKALEEG"
     gene            50001..50279
                     /locus_tag="VBE22_000225"
     CDS             50001..50279
                     /locus_tag="VBE22_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201704.1"
                     /translation="MTVKEYNLRIKGYLLKRLESERDIYLSAFVNRNVKAANSKGEYV
                     YREFEEFYDYEKRKKQVLSASVDNTINPKIIDRANRVKELRERGGLNV"
     gene            50272..52920
                     /locus_tag="VBE22_000226"
     CDS             50272..52920
                     /locus_tag="VBE22_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEO7201705.1"
                     /translation="MSSRAYTVQAILKATDTNFTSTMNKVQSAAQATIDKIKSIKDSN
                     ISTLGKIGEYTTMVGQGMQSVGRSLSKYVTLPIVGLGVAAAKTFGDFESQMNRVKAIS
                     GATGADFEKLRKQAIDLGASSVFSAKEVAQGQEMMASAGFNANQILAASPGVMSLAAA
                     SGGDLALASEAAATAVNMFSLNASEATHVADVFAKAAADTNSEVADMAEALKYAGPVA
                     GAMGISMEETAAAIGIMSNAGIKGSQAGTTLRTAITRLAKPTDQMQAVIDGLGLSFFD
                     TNGKMRSLTEITGQLREKMSGLTDQQKSAALSILFGKESLSGMLALINATPGELSKLT
                     EGLKNSKGAADKMANTMNSGLKGAIEQLKGSLETAGITIGGILNPLLQGVIGKIQAVI
                     DWFNKLSPAGQKLAVIIGGIGAALGPLLVIFGTVIIFIGQVTASLEGVSTAFTAIAGI
                     FSIISGPVILVVAAIGAFIAALVVAWNTSETFRNTIISGWKSMVDAVTPLIETLKQLI
                     LSIFTFIQANSATFIGGLKLAWSALVEALSGLFLVITGVVQVGMSVINTTIKVILAII
                     NGDWNAAWTAIKSGVGAIWEGIKNIIIGGLTFLASLFVSMLGVFVSVFSAGWSAIKGI
                     FSVTLKFLVNVVAAGMSAIGNEISSKINTAKSVAILAFNAMKNGISTAINAAKEVVSS
                     VVNRIKNILNSLANINLGAAGRAIMDGFLNGLTSAFEKVKNFVGGIAGWIQEHKGPIS
                     YDRTLLIPAGRAIMGGFNKSLTDSFEPVKKNVSSMASRISEEFQNGLNKLKDINISEI
                     TEGLQSSFDVTHYASFGGLNDSARKLEDDRFVALKDAVLAIRDFANRDVVVTVNGKEL
                     ARTAGDNFTEYQKQKEIMNNRMKGLI"
     gene            52921..54495
                     /locus_tag="VBE22_000227"
     CDS             52921..54495
                     /locus_tag="VBE22_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO7201706.1"
                     /translation="MANFSYKGVDLSPFLNCLNIVRTIGNNRSIATRKINELGEAIQS
                     VSFGAKTIFVTVSFKTKEIGASKFVDTTEPSTYSYENLNKLREKIAGILHSKTTFKLT
                     LPDEPDRYYMAVPKGDIDLKGISDWYDETVIEFYIPDGVAHSTTYKKFLDYTRDGNKL
                     TFKLQNEGNTNALPIIKIKHNSENGYIGIANETGAFALGSSEEEDGTIVHRNEVLFDY
                     SKAIAKALEGAPNVAKLNHMPPSFDTELKRMRIDNILGSGKGGEYVVIGNRGTTPGYT
                     EHVGTRTFIINPDSNGEYTLNEHLWWQQIFIATAQDQKGFLKLCVTGIDDEGNDEFLY
                     GIETYKRKNGFEAEYNFFALDDDGVGWRFYKQFEFQADRNYHNPFSMDRSRAVEIFRE
                     EDKFRIYFNGAHHHATVPSLKGKKSRKIHLAMGTCSDSSKYINYNLFEKVNFEKMGVS
                     HYNNIVNKYQPGDEVIINFENDTVKTKDIDSLQDMVLGSQPISIPPGESELVINVSKF
                     SSTDPDIELLLEERWL"
     gene            54486..58313
                     /locus_tag="VBE22_000228"
     CDS             54486..58313
                     /locus_tag="VBE22_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7201707.1"
                     /translation="MVVITLVIHDAKLHPVLLLDNERQGALNYYDDLWTRQLTTGSSA
                     FEFSVYKKSLLGDNPLNHKYHALNDQAFVSFVHKDKVQLFNIMRVEETETTIHCYCEN
                     LNLELLNEYCNAYKATKAMSFEEYLVQFDILNWGALTIGTNEVKDKKLTLEWTGQDTK
                     LARILSIANNFDAEIEFETQLHNNHTFKAFIVNVYKEYEEGVSYGVGRDRSDIVLRYQ
                     KNVTGITKKLDKRQIYNAIRPYGKKTVKGERVISNPVTRKVTKTVGSNRTYLGGDLKY
                     YGHTIKKANVQSIINYAVQYNILPSGIICQLYLESLWGDSAVGKRDNNWAGISGGAQT
                     RPSGVKVTTGMARPPSEGGTYMHYASVDDFLKDYTYLLAKQGIYNVVGKKNIADYTKG
                     LFRAGGAKYDYAAAGYQSYTNLMTNIRNGINKVTGNILNTIDKLWQTPVQPITAVNVA
                     RRATKTMQALNEATRLKGRRIGSGQCYALSGWYAKKLDGAWIDSSVGGIRGRIGGGMA
                     AALIGTDYNWGAYGWKLDRSPNAGNLQAGGIYNVKANFGAPFYTTQWGHTGIIKSVSK
                     TRVTVLEQNYAGRMYVMENSYEINAFARGLQTVCYPREIAQGMAVNGATTQQVSGGTQ
                     ISYEEVVQEAQTESYEEEQIIYIDNSIYKEWKDENGKVEYYLKNGFLYAPLSRDRYPS
                     VLTGNETRDNWIRKDMEVETDSQDVLISTALKDLKAHAYPAVTYEVDGYVDLELGDVV
                     RIQDDGYEPPLILTARVIEQEISITNPSSNKTKFSNFVEKESQLASDLISDMLRLYDE
                     SIPYDIQLATSNGVAFKNGVGESVLTPNLQKNGKDYDAIYFYKNGDSLIEIGPSLTVK
                     ASDFNHVLNITVEAYVNEELVASTQISFTDTEDGEKGDDGATSWTAWANSKDGKVDFS
                     ITEAKNRRFIGTYTGLTQSTNYLDYKWIDMSANVVIGTQNLLDGTKSFSGSWFTEGTI
                     FETTKISEYPFEFKKWKSGNKVSHTIEFDVKAGVTYTFTAAIARENAGRLYFYLYDLF
                     ANHITSNTPRETIIENVTTDIQMFKVTFVPLRDGKIKPRFAMLASDAGWFMTGGYMLV
                     KGNKSGDWQESEADKASNLDSKADQELTRAQILALEERTAIARENAIAEAMQHTLSEV
                     ETKWKLWYDLNTIDEKQKVANDIAQLFDRTTEFKQLLGEASAKFSFINNETLIGEEGI
                     AIGDKDGKAKLFLSNDSISFVTNGVAQMTLTGDTLTIKNGLFTERIQIGNFVEEVYDR
                     NPLFNVIRAIRNS"
     gene            58324..60336
                     /locus_tag="VBE22_000229"
     CDS             58324..60336
                     /locus_tag="VBE22_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201708.1"
                     /translation="MGTSTFSGTWGNNLTLDVRTSYTQNLVGNYSTVTVKVYEKISSY
                     GYIDYPGERTMTIVVDGKSYNESVNVDINYGQTKELGTYSYRINHNSYGSKPKFNVTV
                     TLPINFSNYGSASVTNSVSLPTIKRASTATIASGNIGSAVAITIKRQSDTFRHTLKFD
                     FKGLTGTIASLVDTSYSWTLPASLSVLIPNDRSGTGTLIVETFTSDSQKIGENKYTFT
                     ATVPDIAAYKPILASISLSDANTLTGSLITGNNYVRNMSKLKVSFGNSAGANGSTISS
                     YNAEIVEKGKAIFGNGSVFDMLDFVGTATIRATVTDSRGLTSEPVDTKINVIDYFLPI
                     VTSAKVVRSQQNPDILQVLPFVKIAPIIVGGIQKNQLKMSVSVAPYNTGIYAVDSGAA
                     TNTWSTISQMSGAPLNLGGTYDKSKSWLVKISVSDNLMSATPIIQPVASEFVLVTKAP
                     SGVAFGKIWEHGIIDAKGDVYVDGNIYCGDKPIQQKQLALNNGGSFRHDDTDLNSLQD
                     TGFYCVFRGANRPTGAGPGYVTVVRHETSNYAYQQFYDRTNKTIFTRVLENGVWSGWS
                     EYVKKDSLPTTIDTGWQSIGNGFSYRQTGSTVTVKYNFATNGIDKLTVGSMPTNLITS
                     DMMFAVTAWTIQLNVLNVQVSADGRILWFNPSKWTVNVKGQIQWTI"
     gene            60350..60676
                     /locus_tag="VBE22_000230"
     CDS             60350..60676
                     /locus_tag="VBE22_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO7201709.1"
                     /translation="MEILNKYPVMLEDKSIAKVNAIVAVDLPHVRGNLTFDLPVDFDN
                     KSFAETLEKCEQIFYDEKYKDKAQSEKMTELSTSTSTGTQTLINLISTLYAKEVLKDE
                     DLIAIG"
     gene            60651..60863
                     /locus_tag="VBE22_000231"
     CDS             60651..60863
                     /locus_tag="VBE22_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201710.1"
                     /translation="MKILLLLVRIFLQEEGIDMMIKLFAIDLYYGRMAWSSFVKKGFS
                     EFINNKTKEQLAIMCDEELLAEILAS"
     gene            60876..61178
                     /locus_tag="VBE22_000232"
     CDS             60876..61178
                     /locus_tag="VBE22_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201711.1"
                     /translation="MTVEQAERIAQSQFVWAILFILLFMIVVGYLVRTSDKREKKLMD
                     FHDQSKSESNKREEWLKGHLDKNTEQLQDISQTIGVVQKEMSYMSDRIGRLEKEEK"
     gene            61180..61515
                     /locus_tag="VBE22_000233"
     CDS             61180..61515
                     /locus_tag="VBE22_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO7201712.1"
                     /translation="MNEFTTQIITASVPIFGIIAGILTHEVKKLLIKKGGEKAVKIAE
                     IVARNAVEAVEQISVEVGIKGQDKLTEAKTAVIDGLSQYNINLTQTQLETFIEAAVKR
                     MNDEWKKGQ"
     gene            61517..62236
                     /locus_tag="VBE22_000234"
     CDS             61517..62236
                     /locus_tag="VBE22_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7201713.1"
                     /translation="MVINIEQAIAWMASRKGKVTYSMDYRNGPSSYDCSSSVYFALRS
                     AGASDNGWAVNTEYEHDWLIKNGYVLIAENTNWNAQRGDIFIWGKRGASAGAFGHTGM
                     FVDPDNIIHCNYGYNSITVNNHDEIWGYNGQPYVYAYRYSGKQSNAKVDNKSVVSKFE
                     KELDVNTPLSNSNMPYYEATISEDYYVESKPDVNSTDKELLVAGTRVRVYEKVKGWAR
                     IGAPQSNQWVEDAYLIDATDM"
     gene            62432..63328
                     /locus_tag="VBE22_000235"
     CDS             62432..63328
                     /locus_tag="VBE22_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7201714.1"
                     /translation="MNNLVLPQNLNKYNITKIVTNFNRLLALSDNRTLTVDMRNIEFA
                     EPSGVISLYNMLTFATKRKDANIKWLICEESSLNKRQRQAMLYLVDCGFFKVFDKLVY
                     KEPELRPTTFEIKFINTEQIAQWKVTDFKNWLQKQTGRTNEFSSICVAVDEIFNNIAD
                     HSKESKGCIFGQYYPKNKEIVIAVSDFGIGIPQSIKRKFKKDEPDNKLIEFALQEGVS
                     AETIPQNRGAGLSNIVNTLTTNKVGNFTIISNCGIVSVSDNKITQSYSSEESYPGTFF
                     EIRIDVSNDNLYDLEEEEEFEW"
BASE COUNT        21848 a         9984 c        12698 g        18841 t
ORIGIN      
        1 aaggaaataa aattattgat agttatgaaa cagatgttaa tcctcatgaa tctttggatg
       61 agcatattgt tcatttgaca ggtattacgg ataagcagtt ggttaaagct ccggattttg
      121 gtcaagtggc gcatcacatt tatcaattga ttgaagattg tatttttgtt gcccacaatg
      181 taaagttcga tgcaaattta ttggctgagc aactattttt agaaggcttc gagttacgta
      241 caccaaggat agatacagta gagttatcac aagtgtttta tccctgttta gaaaaatatt
      301 ctttaggggc attagcagaa agcttaaata ttgaattaac agatgcgcac actgctattg
      361 cagatgcaag agctacagca cagttattca ttaagttaaa agctaaaatt agtagtttac
      421 ctaaagaagt gttgaagacc attttaacat ttgctgataa tcttttgttc gaatcctatt
      481 tattgattga agaagcttat caagaagctg atttcgttaa tcctaaagaa tattatttct
      541 ggcaaggatt ggtcttaaaa aaagaaaaag ctgttggaaa gcctaagaaa ttatctagtg
      601 attttcaagt taacatggct ttgttaggta tggatgctcg ccctaagcaa attgtttttg
      661 ctgacttggt taaagcgcat tttaatgatc aaacaacaac gtttttagaa gctcaatcag
      721 gacttggtaa aacatatgga tatctcttgc ctttattgga tcagtctcaa aaacaacaaa
      781 taatagtatc tgttccaaca aagattttgc aagatcaaat aatggctaaa gaaataaagc
      841 acattcaaga actttttcat attccctgtc acagtatcaa aggaccgaga aattacttga
      901 agttagatgc gttttataag agcctccaag tgcaggatcg aaatcgattg ataaatcgtt
      961 ttaaaatgca actgctagtg tggttgacgg aaacgactac aggtgatttg gatgaaatta
     1021 aacaaaaaca acgattagaa tcttactttg accagctaaa acacgatgga gaagtgactc
     1081 aatcatcact tttttatgat ctggattttt ggaaacgcag ttatgataaa gttgcacaaa
     1141 gtcaacttgt tattattaat catgcttatt ttcttgagcg tgttcaggat gacaaagatt
     1201 ttgcaaaggg gaaggtttta gtttttgatg aagctcagaa acttgttttg ggactggaaa
     1261 atttttctcg tgggcaactt gatattagtc accaattgca ggttattcaa aaaataattg
     1321 atagtcctat tccgttatta caaaagcgcc ttctggaaag tatttcttat gagttaagtc
     1381 atgcagcaga acttttttat cgtcataatt cttttgaatt ttcagagacc tggttgaaac
     1441 gtctaaaaaa tagtataaac gctttagaag tagtaggatt agatgattta caaacttttt
     1501 tcacagctac ttatactaac tattggtttg aaactgataa agtcaatgag aagcgtttaa
     1561 ccattttacg tggggcgaga gaagattttc ttaaattttc agagttcctc cctcccacaa
     1621 aaaagaccta tatgatatca gctactttac aaatcagtcc caaagtctac ttgtcagatc
     1681 ttttaggagg attttcaagt ataagcacag agaagatagc gcatgagaaa aatgccaatc
     1741 agaaagtatg gattgatacc tctatgccta atatacttga tttatcaccg gaacagtatg
     1801 cttacgaaat agccaagcgt ttacaagata taatgacgtt aaagcagcct accttagtct
     1861 tattaacttc taaacagaca atgtttatgg tatctgatta tttggataag tgggagataa
     1921 aacatcttac tcaagataaa aatggcttag cttataacgt taaaaaacgt tttgatcgtg
     1981 gagaaagcaa tcttctttta ggtacaggat ctttttggga aggtgttgac tttgtacatc
     2041 gtgatcgtct tattgaagtt atcacgcgat tgccttttga taatcccaaa gactatttta
     2101 tccagaaact atcgcagagt ttaacaaaag aggggaaaaa cttcttttat gactattctt
     2161 taccaatgac tgtcttaaaa ttaaaacagg ccttgggacg tactacaagg agagaagaac
     2221 aaaagtcagc ggtaattatt ttggatagca gattggttat taaatcttat ggacaaacca
     2281 tcatgcattc tcttggtaga gattttgaaa tttctaagga aaaaattaac aaggtcttga
     2341 ctgaaatggc taaatttttg atataatggt aaagatcatt gtctaaaaat aagattttga
     2401 cagtgcgact caagattaaa taatagtagt acgttgataa aaggaggagt gatgacctat
     2461 ttgtcagaac gtgtactcaa tatggaagag agtgttactt tagctgcggg agctaaagca
     2521 agagaactca gagtacaagg aagagatatt cttagtttaa ctttagggga gcctgatttc
     2581 gctactccta aaaacattca gcaagcggct attgaggcta taacagatgg acgtgcaagt
     2641 ttttacacac catcaagtgg tttgccggag ttgaagtcag caattaatgc gtattttgaa
     2701 cgtttttatg gctactccct taaacctaat caggtggttg tgggaactgg tgctaagttt
     2761 atcctttata ctttctttat gacagtactg aatcccggtg atgaagtaat tattccgact
     2821 ccttattggg tatcttatgc tgatcagatt aagatggctg aaggtaagcc agtttttgtc
     2881 acagcaaaag aagttaatca cttcaaagta actgtggaac agttagaagc tgttaggact
     2941 gataagacaa aagtcatact tcttaatagt ccatcaaatc ctacaggtat gatttataaa
     3001 gctgaggagt tggaggctat tggaaattgg gcagtcgaac atgatatcct aattttagca
     3061 gatgatattt acggtcgatt ggtttacaat ggcaatattt ttacccctat ttcaagcctt
     3121 tcagagtcta ttcgaaacca aacgatagtc ataaatggag tttctaagac ttatgcgatg
     3181 acaggttggc gtgttggttt tgcagtgggt aatcatgata ttatagcggc aatgtctaaa
     3241 gtagtcagtc aaacaacttc taaccttacg gctgtctcac agtatgctac tattgaggct
     3301 ttaaatggtt ctcaagagtc atttgaaaaa atgaggctag catttgaaga acgccttaat
     3361 atcatttatc cattgttgtg tcaggtccct gggtttgagg ttgttaaacc acaaggtgct
     3421 ttttatcttt tcccaaatgt tacaaaagca atggaaatga aaggatatac ggatgttaca
     3481 gcattcacgg atgccattct tgaagaagtt ggacttgctt tagtaacggg tgctggtttt
     3541 ggagcgccgg aaaatgttcg tctgagttat gctacagatc ttgaaaccct taaagaagct
     3601 gttcgtagac tgcatgtctt tatgggaagt aatgaaatta attaaataaa aatagagaga
     3661 aacttatgtc aaaaaaactt atttcaattg ttgatgttaa agattatgtt ggtcaagaag
     3721 ttactattgg tgcttgggtt gctaacaaat caggtaaagg taagattgcg tttgtacagt
     3781 tgcgcgatgg ttctgccttt ttccaaggtg tcgcttttaa accaaacttc attgaaaaat
     3841 atggtgaaga atcaggtctt gaaaaatttg acgttattaa acgtctcaac caagaaacat
     3901 ctgtttatgt aacagggatt gttaaagagg atgaacgttc aaaatttggc tatgagcttg
     3961 atattactga ccttgaagtc attggagagt cacatgaata tcctataact cctaaagagc
     4021 atgggacaga cttcttgatg gacaaccgtc atctttggtt gcgttctcgt aaacaaatgg
     4081 ctgttatgca aattcgtaac gctattattt attcaactta tgaattcttt gaccaaaatg
     4141 gttttatcaa atttgatagc ccaattttat cagaaaatgc agcagaagat tcaacagagt
     4201 tatttgaaac agactatttt gggaaaccag catttcttag ccaatctggt caattatacc
     4261 ttgaagctgg agcaatggct ctaggccgag ttttcgactt tggtccagtc tttcgtgctg
     4321 aaaaatctaa aacacgtcgc catttaacag aattttggat gatggatgct gagtattctt
     4381 tcttgtcaca tgaggaatca cttgacctcc aagaagctta tgttaaagca ttgatccaag
     4441 gcgttctaga tcgtgcgcct caagcacttg atattttaga acgtgatgtt gaggctctca
     4501 aacgttatat cgcagagcca ttcaagcgtg tttcttatga tgatgctatt acacttcttc
     4561 aagagcatga agcagatgaa gatacagatt atgaacattt agagcatggt gatgactttg
     4621 gttcaccaca tgaaacttgg atttctaatt actttggagt gccgacattt gtagtgaatt
     4681 acccagcaag ctttaaagca ttttatatga aaccagttcc tggaaatcca gaacgcgttc
     4741 tttgtgctga tcttctagct ccagaaggtt atggagagat cattggcggc tcaatgcgtg
     4801 aagacgacta cgatgctctt gtggctaaaa tggatgaact aggtatggat aaatcagaat
     4861 acgactttta ccttgacctt cgtaaatacg gctctgttcc tcatggcggt tttggtatag
     4921 gtattgaacg tatggtaact tttgttgctg gtaccaaaca tattcgcgaa gctattccat
     4981 tcccacgtat gttacatcgt atcaaaccat aataaaaaag cgctagctag tgcgcttttt
     5041 tattatggtc tttagaatcg ttggttaatt aaattgggac tatgtttttt taacaatgtg
     5101 attgtcgaaa agacaaggat aagactgatt agtcgatcca tataatctgt tccaacttga
     5161 acaagtatca aactacttgt catgtctaat ccaattccat gtaagaattg tgctataata
     5221 cttgagccac tagaggtaat tcctttaaaa aggataactg ttataactga agcaataata
     5281 gttcctggta aagaaataat cagtgatttc cacaataggc tagagggttt acaattcctt
     5341 ttaatcaaga tacccgtaat gatagcaacc acgatggcga ctggtgaata ataaagtgaa
     5401 aaaatatcag tagtcatcca attgatgagt gcactgagaa aagcagtgct ggcagcgtac
     5461 catggtccca ggataactgc tattaacaat gtacctatag tatctagata gataggtagg
     5521 cgtaaaaata gagctaaatt agccccaaca aggttgatgg cgatagcgat tgagataaat
     5581 gaaattaaac gagcaggtga ttttgtcatt tgttaagtcc taacatttct aagtcttcaa
     5641 tgatgactga attttgtgca tgtaatagga cggtcataaa atcattccaa aataaattag
     5701 tattgactga ggttaaaatc ttactgttag catctttttt ataaaaatga tactgatcta
     5761 caattgtttg tcccatggcg attccgtgac aagcaacatc ggtatagcta tcaaaaccag
     5821 tagcgatatt ttcattaaca aagtaagcaa tagctaaagg atcatttatc acacatccaa
     5881 taatatgctc atattgccag tgaaaatcga agtagaactt tgtgattttt tggataaaac
     5941 ttgatacgtc tggattaata cgctccatat aagataaatg attgggggtt aatacaatat
     6001 gacgtgtgat gtctagtcca accatctcta tttttttgtc aaggttttca aatacatatt
     6061 gtgcagcatg aggatcacac caatagttgt attcagcaac tggagaacaa ttgccatgtg
     6121 atttaaaact tcctcccatg ctaataaatc gcttgcaatg tttgcctaat ttgggattag
     6181 tttgtaaggc tcgagcgata ttggtcagag gtccgagagc tataatagaa gtatcatttt
     6241 tgtgttcaaa atagtttgct aaaaaacaat ctgctgattc ttcttgaaag ataattggtt
     6301 gcgccaatgt aaagttattc tctccaaggc catccattcc atgagtatcc tgagcagata
     6361 caaattcacg ttgtaaaggc aagttatcac caacataaac tggaatgtcg tgacggttta
     6421 gtaattcaag cgttacaaag gtattcttta gtcctagctc aacaggcgaa ttgcctgctg
     6481 ttatagtaat agcaacaact tctaacttag gatgctgaat agcatacatc agtgctaagg
     6541 tatcatcaat accgggatcg caatcgataa taattttttc tttattcatt tgtctccttt
     6601 cttttaatat tagtgaatgt tctatgcttt tatgaaacta ttaacaatag tcatggcaat
     6661 aaaaaagatc ttggcagtta ccaagatcac acgaaaaaat gaatttagtt ttctaaattc
     6721 aatcatattt tcagtcactt agttttttat tctgggaagt tttcgaacca gtccgagaaa
     6781 tactcgatta tgcacgtgtt ttattataat atgaaagcac taacttgtaa agattcaaac
     6841 tgaccattta acagtgtcta gattgattgt tttagcattt gaaattaatt ttgaaatcgg
     6901 acatctgctg tgtgctttgt taacgattag cttactatca gataatgaca gtttttcaat
     6961 actagcaaca gcgttaactt ggaaataaaa tcccttacct acttgctttt ccttcatgag
     7021 ctggcaagta acatcaacgc gagattttag gtctttgaat cctttttgtt ctaaaatagc
     7081 ttttatagtt gcatttaaac atgttgacca tgctaaagca attaattcct caggattaaa
     7141 tcctggtaaa tgatttaaag ggtgattggt ctccacaatt tttccattgg agagataaac
     7201 ttttccatca acgccttgag tgtttgatga ggttacttca taaagcataa taaactcctt
     7261 tgggtctagt ttaccaaatt tgggagcatt ccgacaagat tttactattt agaatgagtt
     7321 tcttttcctc tgaagtaaaa tcctaattgc tagccatttg tggtataatg gcataaaaca
     7381 taagagaaag aggtaatcgt aatgtcagac gaacaaatca aacttgtgat tgtaacaggt
     7441 atgagtgggg caggaaagac agtggctatt cagtcatttg aagatttagg atattttaca
     7501 atagataata tgccgcctac tctagtgcct aagtttttag aattagcagc acaatcagga
     7561 gatacaagca agattgctat ggtagttgac atgcgtagtc gtcttttctt tagagaaatt
     7621 aactctattt tggatagcct tgaaattaat gataatatta actttaagat tctattttta
     7681 gatgctactg acaccgagct agtgtcacga tataaagaga cacgtcgtag ccaccctctt
     7741 gctgccgacg gcagagtgtt agatggtatt tctctagaga gagaactatt agcaccgctt
     7801 aaaagtatga gtcagaacgt tgtggacact agtgaattaa caccaagaca actacgtaag
     7861 gtcatttctg aagaattctc aaatcaagat agtcaaagtt cttttcgtat cgaagttatg
     7921 agttttggtt ttaaatatgg tatacctttg gatgcggatt tagtattcga tgtgcgtttt
     7981 ttaccaaatc catactataa accagaatta agagataaga caggtttaga tactgaggtt
     8041 tatgactatg tcatgagttt tgatgaatca gatgattttt atgaccatct tcttgcctta
     8101 ataaagccaa tcttaccagg ctatcaaaat gaagggaaat ctgttttgac tgtggcaatc
     8161 ggatgtacag gtgggcaaca tcgtagcacc gcttttgctc atagattatc agaagattta
     8221 aaagcagatt ggacagttaa tgaaagccat cgtgataaaa ataagcgtaa ggagacggta
     8281 aatcgttcat gagaaaacct aaaataaccg ttatcggcgg aggaacggga attccagtta
     8341 ttttgaaaag tcttcgctta gaagatgttg agattacagc ggttgtcacg gttgcagatg
     8401 atggtggttc gtcaggtgag ttgcgctcag ttatgcaact aacaccacca ggagatctaa
     8461 gaaatgtttt ggttgccttg agtgatatgc cgaagtttta cgaacaaatt ttccaatatc
     8521 gttttgcaga aggtgatgga gattttgcag gtcatccttt aggaaatctt attattgctg
     8581 gtgttgctga aatgcaaggt tcaacttata acgctatgca gagtttaaca cagttctttc
     8641 atacaacagg aaagatatat ccatctagtg aacatccgct cactctgcat gctgttttta
     8701 aagatggcca tgaagtggtt ggagagagtc aaatagccga ttataaaggc atgattgacc
     8761 atgtgtatgt cactaatact tataatgaag agactcctac ggccagccgc aaagttgttg
     8821 atgcaatttt agaaagtgat atgattgtgt tgggtccagg gtcacttttc acctctattt
     8881 tacctaacct agttattcca gagattaaac aagctctttt agaaactcgg gcagaggtag
     8941 cttatgtatg caatattatg acacaacgtg gtgaaactga gcattttaca gacgcagacc
     9001 acgttgaagt attgaaacgt catttaggac aagatgctat cgatacagtt ttggttaata
     9061 ttgaaaaagt gccagagtct tatatggaaa acaatcattt tgatgagtat ttggtacagg
     9121 ttgaacatga tttttcaggg cttagaaaac atgccagacg tgtcatctcg tctaactttt
     9181 taaaattaga aaaaggtgga gccttccatc atggtgactt cgttgttgag gaactaatga
     9241 atttagtagg aacacattta tgagttttac agtaaaagtc aaagaagagt tgctaggtca
     9301 taagagtgaa aataagatgg aactgtcagc tattatcaaa atgtcaggta gtttaggatt
     9361 agctaaccac ggcctcaacc tttctattac cacagaaaat gctaaaattg ctcgtcatat
     9421 ttatagcatg ttagaagaac attatcattt gcagccagaa atcaaatacc atcaaaaaac
     9481 gaacttacga aagaatcgtg tgtatactgt ttttattgaa gaaaaagttg atgttattct
     9541 tgctgatttg aagttggctg atgctttttt tggtattgaa actggtattg aacactctat
     9601 attggacaat gatgaaaatg gaagagctta tctcagggga gcattcttat caacgggtac
     9661 tgttagagaa cctgattctg gaaaatatca actagaaatt ttttctgttt atctagacca
     9721 tgcacaagac ttagctaatc ttatgaaaaa atttatgtta gatgcaaagg ttattgaaca
     9781 taaacatgga gcagtaactt atttgcaaaa agcagaagat attatggatt tcttgatagt
     9841 cattgatgcc atggaagcta gagatgcttt tgaagagatt aaaatgatac gtgagacacg
     9901 taatgatatt aaccgtgcta acaacgttga aacagctaat attgctagaa caatcacagc
     9961 tagtatgaag actatcaata atattatcaa gattatggat actattggtt ttgatgcact
    10021 gcctagtgat ttaagacaag tagctcaggt gcgtgtggct catccagatt actctattca
    10081 acaaatagca gacagtttag aaacgcctct gagtaagagt ggcgttaacc atcgcttacg
    10141 gaaaataaat aaaattgcag atgaattata gaaggagata ttagcatggc ttgtacaaca
    10201 atattggttg gtaaaaaggc ttcttatgat ggttcgacta tgatcgctag aacggaagac
    10261 tctgttaatg gcgatttcac acccaaaaaa ttaaaggtaa tgacatctaa agatcaaccg
    10321 cgtcattaca aatcagtttt atcaaatttt gaagtagatt taccagataa cccacttcct
    10381 tatacttcag taccggacgc attgggaaaa gatggtatat ggggtgaagc cggtattaac
    10441 agtaaaaatg tagcgatgag tgctacagaa actattacaa cgaattcccg cgttttgggt
    10501 gcagatcctt tggtttcaga tggtataggg gaagaggata tactcacttt agtgcttccc
    10561 tatattcagt cagcgcgaga aggtgtggag cgtttaggtg ctattttgga aaaatatgga
    10621 acctatgaat caaatggtat tgctttttca gataccgaag aaatatggtg gttagaaaca
    10681 attggtgggc atcattggat tgctcgtcgc gtacctgatg atgtttatgt tactaatcct
    10741 aaccaactag gaattgatca ttttgaattt aataactgtg atgactacat gtgctctagt
    10801 gatttgaaag agtttatcga acaataccat ttagatttga cctattctaa tgagcatttc
    10861 aatcctcgat atgcttttgg tagccaacgt gataaagatc gtcattacaa cacaccaaga
    10921 agttgggcaa tgcagcgttt tttaaatcct gaaattgaac aggatccacg tagcttgttt
    10981 attccctggt gtcaaaaacc ttaccgaaaa attactgttg aggatattaa atatgtgttg
    11041 agtgatcatt atcaagacag tgtgtatgac ccatatggac cagaagggga tgcggtaagt
    11101 aggagagctt ttcgttcagt tggtatcaac cgaactagtc aaacgtctat tctacaatta
    11161 cgaccaaata aatcacttga aacgacaggt gttcaatggt tatcttatgg ctctatgcca
    11221 tttgcaacca tggtgccgtt gtttacacaa gttgagactg taccagacta tttttcgaat
    11281 acaaccaagg atgcttcaac agataatttt tattggacca atcgtttaat tgcagctcta
    11341 gcagatccac acttttatca acatgaagct gatattgaaa gctatatcga gagaacgatg
    11401 gcccaaggac atgcacatat taacggtatt gatagagaag ttgctgagaa taaagagatt
    11461 gattttcaac agaaaaatca agagatgagt gactatattc aaaaagaaag ccaagaattg
    11521 ttaaatcgta ttctatttga tgcaagtaat ttaatgacaa atcgcttttc aatgggagat
    11581 taaaaaactc ggaatatttt ccgggttttt taagataaaa acgcttgcat aagtatataa
    11641 aacttgcaaa aaaaaaatat cgtgctatac tttactagta aaatattaac cggtaaataa
    11701 cgggttaaat aaaaaggaga aaaaatgaga aagaaatttc ttttattgat gagctttgta
    11761 gctatgtttg cagcttggca acttgttcaa gttaaacaag tttgggctga tagtaaactt
    11821 aaagtggtaa caacttttta cccagtttat gagtttacaa aaaatgtcgt tggtgataaa
    11881 gctgatgtat ctatgttaat taaagcaggt acagagccgc atgattttga accatcaact
    11941 aaaaacatcg ctgccatcca agattcaaat gcttttgttt acatggatga taacatggaa
    12001 acttgggctc caaaagtagc taagtcagtt aaatccaaaa aagtaacaac tattaaaggt
    12061 actggcgata tgttacttac taaaggcgtc gaagaagaag gtgaagatca tgaacatgaa
    12121 ggacatggtc atgaagggca tcatcatgaa cttgacccac acgtatggtt gtctccagaa
    12181 cgtgcgattt ctgttgtaga aaacatccgt aataaatttg tcaaagctta tccaaaagat
    12241 gcagcttcat ttaacaaaaa tgcagatgct tacattgcaa aattaaaaga tcttgacaaa
    12301 gaatacaaaa atggtttgtc aaatgctaaa caaaagagtt ttgtgactca acacgcagcg
    12361 tttggttaca tggcgcttga ttacggttta aatcaagttc caattgctgg tcttactcca
    12421 gatgcagaac cttcatcaaa acgtttaggc gaattagcta aatacatcaa gaaatataac
    12481 atcaactaca tttattttga agaaaatgct tcaaataaag ttgctaaaac tttagcagat
    12541 gaagttggcg tgaaaacagc tgtgcttagt ccacttgaag gactttctaa aaaagaaatg
    12601 gcagctggcg aagattactt ctcagttatg agacgtaatt tgaaagttct taaaaagaca
    12661 acagatgttg caggtaaaga agtagctcct gaagaagata aaactaaaac agttgaaaca
    12721 ggttacttta aaactaaaga tgttaaagac cgtaaattga cagattactc tggtaattgg
    12781 caatcagtat atcctcttct tcaagatgga acacttgatc cagtttggga ttacaaagct
    12841 aaatctaaaa aagatatgac tgctgcagag tacaaaaaat attatacagc aggttacaag
    12901 actgacgtag aatcaatcaa gattgatggt aaaaaacatc aaatgacctt tgtacgtaat
    12961 ggtaaatcac aaacatttac atacaaatat gcaggttaca aaatcttaac ttataaaaaa
    13021 ggtaatcgtg gagtacgtta tctctttgaa gctaaggaaa aagatgctgg tcaattcaaa
    13081 tatatccaat ttagtgacca tggtattaaa ccgaataaag ctgaacactt ccatatcttc
    13141 tggggttcag aaagccaaga aaaattattt gaggaaatgg aaaactggcc aacatacttc
    13201 ccagctaaaa tgtctggacg tgaagttgcc caagacctta tgtctcatta atatttgaaa
    13261 gatgatatcg gatacgatat catctttttt tcttcgtacg tgataggctc aaacatagaa
    13321 aaaagcactt ctaatagaag tgcttttaaa attaatgcta tgcttattga gcagcgtttg
    13381 cgtctttgag accatatttt ttgttgaaac ggtccacacg tccatctgct tgagtgaatt
    13441 tttgacgtcc tgtatagaat ggatgtgaat ctgatgagat ttccacacgg ataagtgggt
    13501 aagtttcacc ttcgaattct actgtttctt tagttgattt agttgaacca ctaaggaatt
    13561 tgtaacctgt agttgtatct aagaaaacaa ctggacgata atctggatgg atgtcttttt
    13621 tcatctttaa aaatttcctt tctgccataa ctcgtctgag tcatagttat taactactct
    13681 attctatcaa ataagaatag ccttgacaag tgttttgata aaaaaagtaa aattataaag
    13741 aaagtacttc ctggatttct tggtaaatag cttgattctc ttccaaacta taagaattag
    13801 ctccactggc taaaggatgt ccgccaccat tatgacgttt agctatttca ttaatgatgt
    13861 gtgacttact acgtaggcgc acacgataat gcccatcgct ttgttcaacg aaaattgccc
    13921 aactttctac aatatcaatt tttccaggta caccaacgat tgcagcagtc tctgcatctg
    13981 ttacatcaaa gcgttttaag tcttcttgtg ttaaagtaac gcatgcagcg ccgttcttgt
    14041 caattttaag ttgttcaaaa atgaagcctt gtaattttgc aattttgaaa ggaaaggaat
    14101 ccatttgacg tgccattgca gaaaaatcaa agtcaaattc acgaagcttg ctagcaatct
    14161 tcagtgtttt acttgtggta gcaggatata aaaagcgtcc tgtatctcct acaataccat
    14221 tatatagaac tcttgctgca cttgttgata acaacaaatc acaagaaaga gcaaagtctg
    14281 tcacaatttc acttgcacta gaggcgttag tatctacata agagagatct ccatagattt
    14341 catcgtttgg atgatggtca attttaatta aaaagtcacc ttttttataa cgttcatcat
    14401 caatgcgtgg ggtatttgct gtatctgtaa caataacaag agcgccttga tagtcttggt
    14461 cggtcacttg atccatttta gcaatccaag tgagtgtggg ttcgtcaaaa ccagtagcaa
    14521 gtactttttt ctttgggaaa ttatgcctga tgatatccct taagccgatt tggctaccaa
    14581 gagcatcagg gtctggtctc atatgtcgat ggataatgat ggtgtcgtac tctttgattt
    14641 tatcaagaat ttgttgaaaa ataatcattt tttacctacc taactgtttt gtcttttagt
    14701 gaagaacaag gctatatcgt tgactatatt atagcttaaa tcttttattg aggaaagaaa
    14761 ttaagatagc ggtcacaatg tttgtttatt tttatatgat gatatgttat ttttttataa
    14821 aattcggata ctgctaaata ataatctttt ttataataat attttgtaaa tgtattgctt
    14881 tttagctaac ttttgttaat atagtaaaaa tgctttttaa taggagataa attatgacac
    14941 aagcagtatt aaaagaattg gctaaggcgg agttgcattg tcatctagat ggctcacttt
    15001 ctttaccagc tattagaaaa ttggctaata tggctgatat aatactgccg agtagtgata
    15061 aagaattaag aaaatatgtt atagctccag ctcaaacaga aagcttagta gattacctta
    15121 aaacgtttga gtttatcaga ccgcttcttc aaacgaagga agcattacgt tttgctgctt
    15181 atgatgttgc tagacaggca gctttagaaa atgttattta tattgagatt cgttttgcgc
    15241 cagaactctc tatggataaa ggtttaactg caagtgatac agtccttgca gtcttagaag
    15301 gtttagcaga cgctcagaaa gaatttaata tcgttgcacg cgctcttgtt tgcggtatgc
    15361 gccaatcgtc tcataaaacg accaaggaca ttattaaaca cattgttgat ttggctccca
    15421 aaggcctggt aggttttgat tttgcaggtg atgaattttc ctatccaaca gatagtttag
    15481 tagatcttat tcaggaagta aaacgctcag gttacccgat gactttgcat gcaggtgagt
    15541 gtggttgtgc taagcatatt gcggatagtt taaatttagg gattaagcgt atgggacatg
    15601 tgaccgcttt aacaggacag agggatttaa taaagcgatt tgtcgaagaa gatgctgtag
    15661 cagagatgtg tttaactagc aatttacaaa cgaaagcagc ctcaagtatt caaagttttc
    15721 cttatcaaga actttatgat gctggaggta agattacaat taatacagat aatcgtactg
    15781 tttccgatac aaatttgacg aaagaatata gtcttttcgt aacttatttt ggaacgaaaa
    15841 tagaagactt tcttgttttt aatcaaaacg ctgtgaaagc ctcctttaca agtgattcag
    15901 agaaagatac tttactacat aagctacaag aaaactatga cagctatcta aaataatctt
    15961 cacgattctt taaaaatatg atagaatagt ggtaatttac aagtggagga agatatggct
    16021 ttagcaaaga ttgtttatgc tagcatgacc ggaaatactg aagaaattgc tgacattgtt
    16081 gctgataaat tacgtgactt gggtctagat gttgaagtag aagagtgtac gatggttgat
    16141 gctgcagatt ttgaagatgc tgacattgca attgttgcca cttatacata tggtgatgga
    16201 gatttaccag atgaaatcgt ggacttttat gaagatttag cagaagttga tttatctggt
    16261 aaagtttacg gcgtagtagg ttcaggtgat actttctatg attacttctg caaatctgtt
    16321 gatgagttcg aggctcaatt cgcattaaca ggagcacaaa aaggtgctga ttgtgttaaa
    16381 gttgatttag cggcagaaga cgaagacatt gaaaacttag aagcttttgc tgaagaaatt
    16441 gcttctaaac ttaactaagc taccaaaaag acgaacacga gtcgtctttt taactttttt
    16501 accatctttt gtaactagaa tatgagatat agatatggaa ttagaagaga ttcgtcaaga
    16561 aatagatgaa atagatcagc aattggtaag ccttttagaa actagaatgg gtctaatttt
    16621 agaggtcata gcatttaaaa agaaacaccg acttccagtt ttagataata atcgtgaaaa
    16681 cgaagtgctt aacaatgtat tgaagaaagt ccaaaatcat caatttgacg atgtgatacg
    16741 agcaactttc aaagacatta tgacagaatc tcgtgtttat caaaaggaga atattgtaga
    16801 cggtgattaa acgatttgat aattatgctc agtacgtact tgttttaggg tttagcttac
    16861 taatcggtgg tgtggttggg gctattacag cagtctttgg acgtgttcta ttattcctga
    16921 cagcctttag aagtgattat atagcttacc tactcccttt tcttagcatt gttggtttat
    16981 ttatagtttt tgtctatcaa aaatttgggg gtaagagtgt taaagggatg ggattggtat
    17041 ttgaagtagg tcatggtaat gaggagacta ttccgaaacg tcttgttcct ctcgtgatat
    17101 taacaacttg gttaacccac ttatttggtg gtagtgcagg tcgtgagggg gttgctgtac
    17161 agattggggc taccgtttca cactactttc aaaaatattg ccgtttacaa aacgcttctc
    17221 aacttttcct agtaatggga atggcagcag gttttgctgg tttatttcag actcctttag
    17281 ctgctacttt ttttgcgatc gaagttttgg tagtaggtcg tttaatggtt tcgtatatat
    17341 taccaagttt aattgctgct ctgacagcaa atttcgtctc acattcttta ggtttagaaa
    17401 aattttctca tagtatagca acatcaatgg cgttaacacc agatattatt ctaaaattat
    17461 tggttttagg actatgtttt ggtctgtgtg gcaatttatt tgcttattta ttggctaaag
    17521 ctaaattaat tgcttctagt aggttactaa acccatataa acggattttc acacttggat
    17581 tgttagctac ttttttattg tttatcttcc attttggacg atattcagga ctgggaacaa
    17641 atctaattga ggctagcttc actaataaga atctttatga ttatgattgg ttgttaaaat
    17701 tgtgtttgac agttattact ttggctgcag gttatcaggg aggagaagta actcccttat
    17761 ttgcaatagg agcaagttta ggagtgatta ttgcgcccat tttaggtttg ccagttattt
    17821 tagttgctgc tttaggctat actagcgttt ttgggagtgc aacgaatact ctgttaggtc
    17881 ctatcttaat aggtggcgaa gtctttggct ttgccaacac tccttatttt gttattgtat
    17941 gtcttgttgc ttactccata tcacatgctc atacgattta tggtgctcaa tctagatagt
    18001 tagtgatgag ttttaaaaga gtttatcagt ttatagaacg gaatattccg ttctcttttt
    18061 tattctctta aagtttataa aaatgaagtt tgtaaatttt atgaatgcaa aaattatgtg
    18121 gctaatataa ttttcttcaa agaaattgat tttatgattg tcattaaaat actgtaaaat
    18181 ccaattgaat gtgtaagaac ttaggttagg ataatgtcta caagcattta agtcgcgatg
    18241 gtgaaatgca ttagaagata aaaagttgat gtggatttca actttatggg aaagagtcag
    18301 gtgacacgaa agctaatata gaaaacgtag tttaaaaaat catataatat taaatgaatt
    18361 aaactctcaa aaatcagaaa aaagctaacc tagaaacagt aaatcaaatt tcaataatat
    18421 acaaataact attctaaagg atgacacctt gttcttctag ataaaatagc tttacaagta
    18481 ggttaaaatt tggtagaatt gtgagagtgt gtaatggacg cacaaaaaac ggctaatccg
    18541 ctgagacaag tacttaagat tagtaagaga aggagaataa aaatgaatcc attaattcaa
    18601 agtttgacag aaggtcaact tcgttctgat atccctgagt tccgtgctgg tgatactgta
    18661 cgtgttcacg ctaaagttgt tgaaggtact cgcgaacgta ttcagatctt tgaaggtgtt
    18721 gttatctcac gtaaaggtca aggaatctca gaaatgtaca cagtacgtaa aatttctggt
    18781 ggtatcggtg tagagcgtac attcccaatt cacactcctc gtgttgataa aatcgaagtt
    18841 gttcgttatg gtaaagtacg tcgtgctaaa ctttactact tacgcgcatt gcaaggtaaa
    18901 gctgcacgta ttaaagaaat ccgtcgttaa ttttgatgat cagattttaa aaatgcttgg
    18961 ttgtttgagg atagtaacta tgttttaaaa ctggacaacc aagacgtaaa aaatctgcct
    19021 gtgggcagtt tttttactag gtccccttag ttcaatggat ataacaactc cctcctaagg
    19081 agtaattgct ggttcgattc cggcagggga catgtaaata acgtcaaaag cctttgtatt
    19141 aaaggctttt tgttttattc cgattttaaa aggggcacaa aaggggcagt ttgtttattt
    19201 ataatttctt tcatatttac agttgtgtga ctgtaaatag ataatgttgt ttttggatcg
    19261 ctgtgaccaa ctctatccat tatcgcattt agcggtatcc ctttttctgc taaaaatgat
    19321 atatgcgagt gtctaaataa gtgcgtgtga taatctccat aaattttcaa tcgcttattg
    19381 atgtacgcgt ttaaaatcgg tacaccgttc gaatttggaa agacgaatga acttgtcttt
    19441 ttctgtctat tgacgatatc taaaatatta tctgatacag atattttgcg tgttgatttt
    19501 ttggtcttgg tcgtagtgat ttctcgtgtg ttaaaatcgt aagttgcatt aatcagaatt
    19561 tctttatttt caaaatctat tttgtcgtaa gtcagacaag ctaattctcc ataccgtaca
    19621 cccgtcaaaa acatgaataa aacgatgtct gcgagcgttt gttcgtcatt gtctatcatt
    19681 ctattgcaca ggtcgtaaac ctcgttagat gttaaataaa gcaccttttc aggcttgtaa
    19741 tcatcctttg gtttgggaac taagacgttc tccgtcggat tacttgtcat atagtccatt
    19801 tgtatcgcat aagaaaatat agcatgcaat ctctttctac atttatgcgt aacatgatac
    19861 gaattgtgtt ttaatagttt atcaatgatt agtctaatat cacgtttagt caacttgttg
    19921 ataattgtat catctggtaa cacggttgct atatgactgt cagatactaa ataaccacgt
    19981 tttgtggagt ctttgacggt cggtatccat tgctttagat attccttttt aagttctccg
    20041 taagtcattt ctgagtgatt tctgatagcg agcctatctt caatcttttc ctgcaatatt
    20101 aaaccagctt ttttctgagc ttgactagaa tttttatcta gtgtcacaga tacttttttg
    20161 tacttatttg ttagagggtc agtatatctt tcgatatatt taaatttccc gttggctagc
    20221 tcctctatcc acattgtttt atacctcatt ttctgttaaa ataggtatgg taaaaacccc
    20281 tcccaaaaag caggttttta ctatactaga atttgcctca cgctctcctt ggccaaaatt
    20341 tgagcgtggg gcttttttta tttgtcttca tcaaattcta aatctaattc aattataccg
    20401 tcttctctag atgtcataac attaaatttc tctttgaaat ctttccagtt agatgaaatt
    20461 ctcataatcc caagaacttt ataaatttga gcgtttaaat caggatgtcc tatatctggt
    20521 gttaaagttt ggaaaaatct agctttatat tgacctttct ttgtttttgg agttcttctt
    20581 tttaattctt ctaacactcc atcaggcagt cttttataaa tcaattcgtt tgtccattta
    20641 ccaacaacac ctggacgcct tttaattgat tgaacatcgt agctccaccc gttcaatcta
    20701 aaaatttcca tataaaaatc atctggaaac atcttttgcc attttagtaa ttcttcacta
    20761 atatatgctt taaatatttt ttgaagctca aaacgttctc tctcatattg ataaccagta
    20821 gcttcatcta ctaaagcagt aacaccaact ttcgcaagtg agcgaactaa catttccgct
    20881 ttttgtgcag ttctcatctg agcttctgat ttgatagctc caatttctct agcttttaaa
    20941 tataaatctg atactagcgg taaaatgtta gcatcaaaac ctaattgaat cctaccattt
    21001 aaatccctat attcaatttt gttgatcaca ccccttaaat cctttgatat caatggttga
    21061 aggttttttg catccataaa agtaggtatt ccgatcacac gggtattgcc tctactaggt
    21121 ctgtccaatg ctttaaaaac accagcttgg gtgatgattc gctgaccgtt atttaaaact
    21181 gctacatcaa tagttgtatt atctaagact aatttaccct cgtgagttgc atgtaaaact
    21241 tcattttcca tattttctcc taaactcatt gacaaccata gcttcgttcg ctattgtttt
    21301 taatttgtaa actttcataa atctgtaaac gttaaagtct ttaacgtcgt ctaacgtggt
    21361 tcagatatta cacatagtta ttaattttcc tatttacgtt tcgactgggg taaaatggca
    21421 cgttatacaa aatgacgttt aaataattta tggctttcaa tgttctgatc aacatctttc
    21481 tcatcccaaa tttgtaactc ccaaggatag tagtggttgc tcttattctt gaaataaacg
    21541 tgtattcctg tgtaattatc tttatctcgt aaataccagt tttttaaatc gaacttatct
    21601 ttccattcat cgagttgctc catcacttgt gaaatatcct cagaagataa aatgatacga
    21661 gcgccaaaaa tatcattaag aatagaattc acaggataac catcttgtct tttggaaaaa
    21721 cgctcaattt tgtctaagat gctttctgat gttttgacac gatagacgta agggatatct
    21781 ttgacatcgg ctttcatcaa ataatcattg atggattcgt gtagatttaa acgatagtct
    21841 aggatagcct tgacaggtac ttttgaaaag gtatgtttta gattaatctt ttcaactttc
    21901 ccagtttcaa aataatcttg cgaataaaca aggtgtattc tattaatttc cgagattaag
    21961 cgttcaactt tttccagcat attaactctc cctataaata tctacgactt ctcctatcgt
    22021 gcggatgttg tcgttctctg aaagatagat ttcctcatag cttttattta aactttgcaa
    22081 gtaccaagcg ccgtcataat cacgtttgag tttttttaca aagttttttc cattgacttg
    22141 gaatatccct atatcattca tatcgacttg actagatacc ttgatgaaca gtaaatcgtt
    22201 gtcttgaata agtggttcca ttgaattacc agctactttc gcaatagtat catacttatc
    22261 ggggacatca tcagcccgta gtttaacttc catatgtaaa ttgtcttctt ggtatgcccc
    22321 cagaccagca gcaactaacc cctcaacgta gtcagtgata taattttctg tctcctcaac
    22381 ttttctatcg aacatagaaa taacattatt ttgttcttct aattgttcgt tagcgaagct
    22441 aaggactttt tcttgtctag gttgttctaa ttgagaagaa gtagaagtga ttttttgtag
    22501 ggtagaggaa gttagatttt tttcagtttt cgtttcattt aacgcgacta tctgattttc
    22561 gtctagcgaa tttaataaat catcaaaaga gatgccaatt gtgtcagaaa ctttctttat
    22621 aatcggaata gatgggatga tttcttttcc gttacgagga tctctgtttt tttcaagaat
    22681 agaaacgtaa gctttgctga tgcctgattc tttagcgaat tcagccatgc tcagattatt
    22741 ggtatctcga tactttttta tatattttcc tagatgcata tcgaattcct ttcaatataa
    22801 tgtctaacat tttaaacata ttatacagta caaaaaaatt tttttcaagt tttttgtcca
    22861 acatacttga caaccatatg tctaacatgt tagactataa tcaagcttaa ggaattaagc
    22921 aaaacgaaag gaggtacagc tgatgaaatc taggctaaac aaaaagccta aacacaaaga
    22981 actagaagtc gaaatcaaga ttctttggtt taagctcaaa atcaaatatc tgattacgag
    23041 gtaatcggat agggggtgaa attcccccac ccctaacggg gtaagtttag tttagcacat
    23101 cggctgtatc tctgcaagaa tgaaaggaga gattatgcca gaggatttaa tcaaacaact
    23161 agaagctggt tcaaaatttc tagcgaagac atgtttacat agcaagatta ttatcacggt
    23221 ggatggtatt cagctggtgg aaacaaaaga gttccaccca agaaatgaac ttctatgagg
    23281 aaaggaattg tatggaggaa ttagaaaaca ttataaaaaa actccaagaa gcaaatagcg
    23341 acccagagtt taataaagcc atcttacttg agtgttctgc tgaattaaca agtttgttta
    23401 atcgcttgtc atatcaacga cattagagtc agtagagata ccttttttaa tagattttat
    23461 tgttcgaatg gcagaatctt taaaatagta agtctcacta gttgccacaa cttcatgatt
    23521 atcagctttt attacaaaat aaaactggtt attagaagat tttctaatca caaaatacat
    23581 agcttatcct cctttctgct aggataagtt gattataaca tttttaggag gtacaaaatg
    23641 aattggaaaa aactaatgtt tggcgatcta gaacacacgt ttactagtcg taatggcaaa
    23701 gaaaaaacaa gtattgaatt tgaaggtggc gtattgccag cactgttagt gctaggtggt
    23761 atcacttggc tgatcgcttg gtttattaca aaataaaaac tcccaagtgg gagtggaaag
    23821 gagaggggtc agaaaaatga ttaatcttga agaaaaagtt caaaaaatag aacagaacga
    23881 agaagaaaat acaacctcaa tctcattctt aagaggttat atcaaaggcc ttgaggagcg
    23941 aattagtcgt ttagagcgtt cgaagggctg ttaaaggcat tagggtattt tgtttgtaac
    24001 tcaaaaatct tttctaactt ttctggctct tgaaaagcca tcataattaa gtcttcctga
    24061 gtcattggtc ttttttgagt agaattattt tctattttat cagataaatt ttcaattgca
    24121 tcatagatgt tgttaattga agtttttaat tggataatat cttgcactga agcactttgg
    24181 tcgtttggtt catggattat agaaaaatca atgttttctt ctgttttttg aattcgtcta
    24241 attgtttctt ggacagaatc aagcacaacc ttactttcag agtttttgag atcgtaatga
    24301 atggtattta tagttctaat atcaaaaggg atatcttcat cagtcttttg tctaaggaaa
    24361 atagtaggta agttcaacgc ttttctaaac ccaagttcta aaaagacatt cgggttattt
    24421 cctgttatat caacaattac caagtctgct gtggataaag catcaaatat tttatcgtca
    24481 atcctatctg aatgatacag ttcgtcagct ctggtcactt gatacttttc gatcaatgca
    24541 ggtttaatta tataatttaa aacactgtct gagtggttcc gaatttcgct actttcctca
    24601 ccgatagcag atactacgaa acaagatttt ttactcataa taatacctca caatttttat
    24661 ttaaattata ccacagaaag aaggtggaat agatgcgccc caaaaaatat ccgtatagcc
    24721 aaaaaaacta tccagcgccg aagcttcata acatcattga aaccgataat catttcttga
    24781 tagatgataa aaggattcat tatgttattg aagattctgt aaagacaaaa acccttggcg
    24841 atggctatgt tgaagtaaca ctttccataa ttgccaagag ttttacaaaa tcacaaggtt
    24901 agtttggcga tagtccttgt gatagaagtt ggtgtaagag ggataccttc ttctttaagg
    24961 acattcttta aacggctcga aaaattatta tctttaagac tttgaagata taagtaacca
    25021 gttgttgtta cgccatcgaa aaaatagaaa gtaatatctt tacattcacc tcctttctgc
    25081 tcacattata gcagattaga ggtactaaaa acagatagaa agggggtggg ggaatgcaac
    25141 aatttaatct aaaacaacta cgagagaaaa aaggatttac tcaaaatgaa ttagctgata
    25201 aagctaacgt tagtcgttcg ctcgtggttg gtttagaaac aggttcatat tcagagacat
    25261 ctacagcatc tctgaagaaa ttggcgaaag ctttggacgt aaagattaag gatttatttt
    25321 tttaaccaat tgtctaacat gatagacaaa atatttgtta ataaaactaa caaaccgcta
    25381 gaaaggacaa tatgaatgaa ttaacattat cggataatct agctcagatt gaactagatt
    25441 taagacaaga aaacgaacag attggaaagt ctatttggaa aataggttgc atgctaaaac
    25501 atgttaaaga aaatgatttg acacatggtc aatttatgga ttggtacaaa aatcttgggt
    25561 acaacaagaa tttcgttagc aaggcaataa cgatagctga caaactttca aatttcccga
    25621 cgttgggaaa tatcggaaca gaagctctct acctcatagc caccctacca gacgagcaaa
    25681 agcaggaaca gattgaacgg attgaaagtg gcgataaccc aactgtcaga gagttgcaag
    25741 aaatcaagcg agaaaacaac cgcttaaaat cagaaaatgc ccgtttagag caacaaaaag
    25801 agaatttagc agagcaagcc ttgagtgcta aaatcgttga aaaagaagtt atcaaagaag
    25861 ttattccaga tgattatgaa agcactaagc aacttaatca gactttgctt ggcaagaata
    25921 aagaactaag taagatggtt gatgatgcct tgcagcacga ggagtattta aaaagtcaac
    25981 ttaaagaatt ctatgctaag cgtgatgagg tcaatcataa atcagctaag tatgatgagc
    26041 taacagaagc gattaaacag tcagaaggta aactcaatag ctatcaaaag aagattgcat
    26101 catacaagaa tatcactgaa ctactcaaaa aaggcgattt gctattactt gaaatgagcg
    26161 gacttatcta tgctgatgaa acgcactata tccagcgtga cgggcttatt aagcaagagt
    26221 tcgatagctt ggtcgataga ggtctaaagc tctttaatga ccttgatatg aagcgtaaga
    26281 acactgaaat tttagaagga gaagtcttat gaatgaacta acaacaactc aacaactgat
    26341 tgaaatgtca aaaatgcaga cagtgacttt agaaaaggtt gataacttag aaaaaggatt
    26401 attgcaagcg cagaatgata ttcaagaaat catggataca tcttatttac atcctggaat
    26461 tattaatatg attactaaaa aacgtcgtaa acgtgtcatt gattgtatgg gcggaaaatc
    26521 tgcaaaagct tataaaacct ttaaagtaga cgaggaaggc agaaagcatc gtttttcaag
    26581 tgaagtattc cgagaaatgg aacttgattt caaagcagaa tttgatctca atagctacgc
    26641 tgaattgtct aaatctaaga aagaagaggc gcttgaatat atcgctatgt gggaaccgtg
    26701 cacgaatact aaacgaaaaa ttaatctgtt gaacaaacag actgaacttg aactgattgg
    26761 gtaagaaagg aacaaaacta acgaagggag aaaagaatgg aagaagacat caagcagtta
    26821 atcactgaaa atatcttaga atttttaggt tctgactatg gaaaaggttt cttaaccggc
    26881 ataaaaattg ccgttgagat tattgccaaa caaataccga caccttcaga agaggatatc
    26941 ggtcatgata gttaattttt gcgctgctgt tcgaaagctt gttttaaatg agttgccatc
    27001 atttgctgaa cagcaagaac gaaatccata ctatggagcg ttcatacaaa atcaaagtgt
    27061 atatcactaa ggagaaagaa catgattact attttaaaag aaataaacca aacactaaaa
    27121 gaaatcctag cagaattaaa agaacctact gtggtaacag tagattctga aaagttaagt
    27181 aatacgctaa ctactgaaca gaagatacgc cgattatctg gtcaataaac agaacaacaa
    27241 atggaaggtt caaagtagat tcttttgatg ttgacaaagt agcatcaaca agcaacataa
    27301 atctttcgtt gtcatcttta ttttctttac gatattccaa gaattcaagg acatgagcat
    27361 acttggcttt atcaggattt tcaggtaata aagttccagt gaatattcca ctcgctgtaa
    27421 tcacattaag cttacaattg cttttttccg aaaaaatagc gacatctgca ataagattag
    27481 tttttaaaac gtcgttcatt ataatcacct cctttcgaga tgattatacc actaaaaaag
    27541 tccgacggga atcggactca aaacaaactt aatttactta attatatcac agaaaggaaa
    27601 caatatgcta gcaaaactta aaagcggtat cgaagtacct tacgaagagc tttggcttaa
    27661 tgataacgac ttatccgaat ttattggaaa gtcatttgac caaacgcagc gattactaag
    27721 aaagatgtac aaagacagaa attatcgcaa atacattgac aaggttggcg gtcgttcaac
    27781 aaaagttaaa aaatttgaag aatggagaaa attacaaaat gaaaaaatta tttaacttta
    27841 tttttacaaa accaaaaaaa gaagaaccaa aaccaaaaag gacaattgaa acacacggct
    27901 gggaagttgg tgcgaaagct tatgatgatt ttcataatta tatgaaaggt ttgaaacatg
    27961 gatgaatgga atccaatttt aattaggaac tagatggttt gcctggtaag caggtttggt
    28021 aggaaataca tagtcctttg acaactgaat atgggtgcgt tggagtaaaa tatattttac
    28081 aaatttccgt tttaatacgg ttaaagtgta ttttttcttg atatttcgtt tttttcgtag
    28141 tatactttag gggaagtgaa gaatatttag cacatttagt gccaaaaaga aatcccctag
    28201 taccgcaaat actaggggat ttttctagtt tcctagaaga cactaatcat cgttgtctag
    28261 ccacttttgg actagcaaca agacgatgcc gaccaataat ggtgcgatga ttgttgtgaa
    28321 gaatatttcg cacatggttc tcacctccaa tctaaggcgg tgtagtagtg ccgagtagta
    28381 ttatagcatt taaagcttct aaaagctacg agatttcaat cctgatagct tctgatgaca
    28441 agaacggtaa aatgtatgtc tagataacag actgtctcag aagtgatttt agggctgttt
    28501 tgagagaaag aaaaatattt aaaaatgttt aaacaaaacc attgacattt gtttaaacat
    28561 aagttataat taaatcatgg ttaaggaatt aactaaatcc aacggaaagg aaacggagct
    28621 atgcgaagtc gaaaaactaa gcaaaaagaa aagctctcgc tgactgataa atttgtgatt
    28681 atcggcataa tcatagaaat aatcagattc atacgagaac ttctctaaaa gcaacgaaac
    28741 cagagggcga aagccctcgg tggtttcgtg tacttcgatt ataacatagc tcctacaggc
    28801 tagcaaatga agcacgaaaa aagaactgaa attttgttag ggattttaat tgtcttgcaa
    28861 ttgttaaatc ttgccttgag ttttcgatag gtgacagatt tggcaaaaat ggggcgacct
    28921 actagcaatc caagggataa gtatatcggt gtcagagctt caaaaaatga ggtagaaatg
    28981 cttgattttt gtaccgaaaa gactggaaaa agcaaaaccg atatcctcat ggaagggctc
    29041 gaaaaagtct ataatgagct aaaagggtaa acaaaaaaac accacgtaac tactcaaacg
    29101 ccaatccgat tagttacgca gtgccaaacg cacccatatt caagaagaat acaggatacg
    29161 cttatattat aacagcgtac ctgtatttgt gcaactcaaa tttataggta cgctttttgt
    29221 gtgcctcaaa ttcgcaggag gactgtatga aaacttacac attaactgaa gaagaattga
    29281 atgaattagt agccgagcgc atgaaacaag cgaaagaaaa acgcacacca caggggctat
    29341 ttaaagatgt cggctttgat gatgagttaa ttccgatcaa caataaatac ccaaaagtac
    29401 tcaagaaatt aaatcgtgaa cgtgcttata aaccagaaaa acacgtcttc aatcagacac
    29461 caaaagtttt tggtgtggac aacgagatta gttatagcaa aattacaaca catgacgtgc
    29521 acaaccatat tcgtttgctt gtcttaaatg tctttggtaa aagtcaaaat aaggaagtat
    29581 tgcctgagga atacgaccaa gcaatagaac tttacaatca attaaaagag tggtttgtgt
    29641 ctagctatga taagcgatta gagggattgg tactagaaga tgattaaaaa aatatgcgtt
    29701 aactacttac taaaacagat tgacaaaagc aaactagaaa caagagataa agcgaagttg
    29761 aactacttta tcacactagt ggactgcaag ttaggaggat aaatggcaca aaaatcaaaa
    29821 acaaaaatct attattggtt gaaatttgat aaacattttt ttgaaaattt atttattaaa
    29881 aggcttttaa gaaatttccc tggcggttcg gacatgattg ttatttacat cagactcatg
    29941 ttagaagcta ttgagaatga ttgcatcatt gattacgaag gaacttttga taattacgca
    30001 gaagaactag ctcttcgtct tgaaacttca gaagaacaga taaatatggc tttagcatat
    30061 tttgtgaaat gtggagtcat tcaagttgat gatggcggta atactcatta tccgcaagca
    30121 aaggctttgt tggggcaaga aacaaactgg aatagataca agaaaaaaca agcagagttg
    30181 gaaaaattcc aaccgctttc caacaaagtt ccaacagaga tagagataga gatagataaa
    30241 gagaaagata aagagaaaga taaagagaaa gaatatattg tcgagcaaag cccgactgaa
    30301 tatctctttc caaattggtt agaagagaaa tatgttgaac aagttaaaaa aggaaaccct
    30361 aaaaactttg actgtcgtat cccaatagct tatctcaacc aaaaaacaaa ctctaactat
    30421 aagtttgtgg atagcaacat taatcttgtt aaaactagac taaaagacgg ctatacgcta
    30481 gatgacttta aaaccgtaat tgataaaaag tgtagcgagt gggggaactc cgacatggca
    30541 aaatatctca gaccatcaac cttgtttaat gctagcaagt ttgagagtta tctcaaccag
    30601 ccagaggcaa gtaatgggga ttattaccaa aaacaacaag ggcaacggtt ctcgcaagct
    30661 gaactggatg aattgaagaa accagacccg aaatatggat tttaggaggt acctatggct
    30721 tttgggttaa tgacaagaga aaacatgctc gagaatggca ttattagaga tactgggaaa
    30781 acatgcgaaa agcacgagat gccaatttat gctaggaaaa tgccaaatca tggcaataga
    30841 gaaacagaat tttgttggca atgtacaaca gagtatatcc aatcgaaaag taatgcggtt
    30901 gacattgcgt acaacaacca gtcgttgcta gctaagggtt ataaagtgtt ttataaagag
    30961 agcgttttat caaaggaaat tgctagtgct acgttgaaaa actacaagga acatagtgct
    31021 gtagatacaa aagcgctaaa ctatgccaaa cgaatcacca gagattatgt taaaggaatg
    31081 gaaggtaact ccctcttaca aggacctcca ggggttggca agagccactt gtctatgagt
    31141 attgctaaaa atattaacga gatgtttaaa tcttacaatc aatcaaagag tgtgatattt
    31201 gtttcggtac ctttgttgtc cggactagtc aaagatacat tcgattatga cgataaaaaa
    31261 aatagcaaat attcgcaaga aagaatgtca aagcttctca tcaattgtga ttatctgata
    31321 cttgatgact taggcaagga gtcaaccaca ggtaacacca ttaaatctgc tagcggttgg
    31381 acatatacgt ttttatttaa tattttggat aatcggacaa atactatcat taatacaaat
    31441 tttagtagag ctgagcttat gaaaatctac gatgctgctt ttgtcgatcg cataatcaaa
    31501 ggtgcaaaaa acaatatttt taaatatcca gataatgcag aaagtaagag gttctgatgg
    31561 aactaacatt aacaacattt ttcagcttgt cagaagaaca tgcagcaaaa atcatggctc
    31621 tagacgaacc tagtcgaaat aaaaaaattg aagaatatag gcagttaaga ctgcgcagag
    31681 ggaggattga ctttggaaaa tagaccagat ttaaaattag tagctgaatt agaaaatagg
    31741 attaaagact taaaaattga aaatgaaatc ttaaagtcta aaaacattga tttgtctgaa
    31801 gatgttaaac atttaaaata taaatgcttc gaaaaagata gttctatagt ggatattatg
    31861 gttaataata acgacctaag aaaagaaaac aatgagttgc gagaaatgtt tgactttttc
    31921 aaagatagac tagagaaatt tgtaggtagt tcgcatggta gaaattagga ttaatggcga
    31981 acttgtaacg tttgacggta attttaggga tgcttttata tttacaattg actatttacg
    32041 agacagcgaa gagcctacgc taaggcagac ttacaacgaa tttaaagact atacagacga
    32101 agacttgatg gaatacattg aaacggaatt tgatgtaaaa cctgaattaa ttgtcaatcg
    32161 gagacttgat agtaaatggg cttttaaatc tcacattttg gaagactaaa tatgagcgaa
    32221 gagttatacg agtctactcg ttattggcaa agtagataca gcgacttgat gtctgattat
    32281 cttaaagaag ctgaagaaaa tatagagtta aagaaacagt tgaagagatt aaaagccgaa
    32341 aattggcaat tgaaacatag aaagaggaaa taaatggctt atttatacga gttacagggt
    32401 atttatgcgc aattatcagc tatggatctt gatgatgaaa catttcaaga cactttggat
    32461 agcatcgatt ttcaatcaga tttagagaat aatattgaat attttgtaaa gatgttaaaa
    32521 aatacacaag ctgatattga aatgtacaaa aacgaaaaag aaacttttta caaaaagcaa
    32581 aagcaagcag aagccaaagc ggaaaaatac aaagatacaa ttaggctagc aatggactta
    32641 agtcaaaaga aaaaagttga tgctggaatg tttaaagtat ctttgcgaaa aagtaagaag
    32701 gttgaggttt tggacgaaac aaaaatacct tttgaataca tgcaagaaaa agttgaatac
    32761 aaaccaaaaa aagatgaaat ctcaaaagtt ttaaaatctg gaattgatat atctggagtt
    32821 caactaatcg aaacagaaag tttacaggtg aagtagatga gcatgacttt tgcagaattg
    32881 cagacaaaaa tgcaaataac aaaaacaaca aaacaaggcg ttaaatatac atttcgcaat
    32941 gcagaagata tttttacaca ctttaaaaca ctaaatagcg ggtgggagtt aacggtatct
    33001 gacgaattgg tggaattgat cggcagaatt tttatcaaag caacagcaac agctagactt
    33061 ggtgatgaac aacaccaagc gacaagatat gctgagttgg acagtgtgcc tgttttaaat
    33121 actaaagact ataaaacagg agaacctaaa caaatacaac aaatgcaagt tccgcaatgg
    33181 actggtgcag tgagttcgta cgcaggtaag tatgccttgc aagggctgtt tggaattggt
    33241 gaggaagatg tagatgcgat tgttacagaa gatacgcaac gcaaagaaca aaaaacctcc
    33301 cgaccgacaa cctctaaaac tcctaaaata agcaatatcc aagtcgagac ttacaagtct
    33361 gatttaaatg atattgcgaa agccacaaac caaaacgttg aagagttaac aaaatggcta
    33421 accgatactt taaaagtggg gacactggaa aatttgcata cggaacacat tgtttcggca
    33481 gacgaattaa tcaataaact caaaaagaaa gcaggactaa aaaatgatta ataatattgt
    33541 acttgtaggt cgcatgacca aggatgccga acttcgtcac acgccaagtc aagtagctgt
    33601 agctacgttt acacttgcag ttaaccgcag attcaaagag caaaacggag agcgagagac
    33661 ggacttcatt aattgtgtta tctggcgaca atctgctgaa aatctagcaa actgggctaa
    33721 aaaagggact ttaatcggta tcacagggca cattcagaca cgcaattacg aaaatcagca
    33781 agggcaacgt atctatgtaa cagaagttgt tgcggaaaat ttccaattat tagaaagtcg
    33841 caatagccaa caacagacta atcaaagcgg caatagttct aattctcatt ttggcaatgc
    33901 caacaaaatg gatatttcag atgatgactt accattctaa atatgacgaa gccgcggaag
    33961 caaaggatat atgcaatata tgacgacgac aagtttgtcg acgttggcac aaaagaagag
    34021 ttatcagcac ggcttggtat caaaaaagca accatagaac agtacatgac taaatcttat
    34081 caagctagac ctagctcaaa aagaatcgct atttttgtag gagttgaaga aattgaattt
    34141 taaaacagag tttgagatac cagtagagcc aaaaccacaa acaagaccaa aatttagcaa
    34201 gtggggaacg tatgaagacc ctaaaatgaa aaagtggaga aaacaagtca ctggttggat
    34261 tgaaaaaaac tataatgggc cattttttga taactgtgta aaagtagatg tcacgtttta
    34321 catgagagcg ccacaaacgc taataaaaga gcctacggca cgctcaaaaa gtaaaaccgt
    34381 acaaatatat caaaaattta taaacgagct tatatggcac gtaaagaagc ctgatattga
    34441 taacctagtt aaagctgttt ttgatagtat ttcagacgcg ggttatgaca aaatacaaaa
    34501 atcggggatt gtctggtcag acgataatat tgtatgtgac ttaagagcaa caaaaaagta
    34561 tagtccaaac cctagaataa aagtaaaaat tgaggaaata gatgaacgaa ctaacggata
    34621 aattttatag tatctttgat agcagtattt tgagacgtgt caaagagtta aatctagatg
    34681 ataaaacatc agaacgctta agactaaata tctcaaataa caagcgtaga aatatactgc
    34741 caaggcctta cgtaatcgaa gcatttaaag attattttga taaagacact tatgtgcagc
    34801 tgtatcttaa atcatacaga gagtatcaca acccaaacag ccatgaaact gatattttta
    34861 taaagttaaa caaaaagcac agagatacaa agttagacca ttacaagaaa gttaagcgat
    34921 tgatgtacgc agcaatgact ttctgaggag gtatagtatg gcagataaaa taaacgcaga
    34981 gagtatgcaa gctgcataca acgaaaatta tcaaatgttt ttggctaaaa atgcagatta
    35041 tgggaactcg ttcgaaaagt ctttgaatga cttcggattt atcgctggtg tcgtccgtat
    35101 aggcgataaa tacaacagac tatataatct tataagcagc gacaaaaacg tctcagaaag
    35161 cctgtcagac acgttaaacg acatggctaa ttattgcaca atgttgtcaa tctggctaga
    35221 gaaaacggag aatgcaaatg acacacgtag ttagggttta cgatcacatt ggtggacgag
    35281 tgttgcctat tactagaagg gttagaagga tgacaaaata tattgaaatt aaagatgatt
    35341 ggaaaaaagc agtagaccat ttaaatgatt ttatcgaaaa gtatagctat aagaagattg
    35401 ttgtcgttgg gtatcaggtt gttagaaacc ccgagacaaa cggaacatgt actcatgtgt
    35461 tagttaaagt ggattgatga aaaaaatatc aaattcgagg taacagaatg acaaaagaag
    35521 aagtaattgc atttctgaca gaacagcgtg atttgcggct tgttgcatat gagtggggaa
    35581 aagacaatct gtccgttttt gcgagatggc aattagaaca agcaaatatg tatttagata
    35641 tcattgagtg gatagaggag gtgacggaat gactgaacaa cagatgattg attgcttgct
    35701 ttatgagtta gcaaaaaaag acaaattgaa cattagacga aacaatatca taacgttttt
    35761 atcgattgtg ctgatggcta tatctatttt aaacgtcgca ctgcaagacc actacaagct
    35821 acaaattaca ggattacgga cacagctaag cagaacacaa aagcaactta aacgtgctag
    35881 cgagcaaaat cagagacaga ctaagcggat tgcggaactt acaggaaatg ggggataggg
    35941 tatgattgac gaaattttaa aaagacttaa taaagaattt gacaatgatc tggataatta
    36001 cgaacaagaa agatacgctg gttacatgga cgcaataggt gtagcaattg aaattgttga
    36061 agaagttaag cgaggtagaa aatgaatatt gaagaagcga agaaattgat agacaaacag
    36121 tctattggta aaggtggtgt cggcgacatt ccagtagtga aaacacatat tgtaaaagta
    36181 ttactcgacc aactcgacaa accaaaacta gaagtaccac aaatgatagc tgatgttatt
    36241 gaaagctttg acgaagatgt gaattatttg cacgaacata tgagttatca gtctgatgaa
    36301 gttagagagt ggctaactca caatgaacgt gagttttatg aagcttggct agcttatcca
    36361 aatatcacag tcgaaaaaga aaatctgtat actgttgaga tacctaatcc gaatgaaaga
    36421 cagttaagtt ttgtgctgat gagacagctt agcggaaatg taagtatcaa agttatgcat
    36481 agagataact tagacttact aaagatagat aacaatttac aactcacaga atccgaaatc
    36541 cgcaaagatt ttgactgggc ttggcagttt agaaaagatg tagaaaatga atgaaagaga
    36601 gttaaataaa aattatgaat tatgataaaa ataaaaatga tgctaaaaag aactttatta
    36661 tagctttagt tctattgccg ttcggcttag tattatctgg atttgttatt aaatacggtt
    36721 ggaataacat tttatcaaca attgatggcg taccatctat caatttaccg caagctgtag
    36781 gaatcaatgt gttaattagt ccttttgctt ctaaaaaaaa tacagatgaa gattttgcta
    36841 cagtgattgc aagagcgttt atttctccgc tagtcgtatt gttattgctt tggatagtga
    36901 ctttgtttat gtaatatctt gaggtaacag aatgaatgaa gaattaggag tgttggtaaa
    36961 tcccaaacgt gcgtattatg taggaaataa tcgggacgga ttaccagtat acacagtcaa
    37021 caccgaatat gcacacaaat gcacacgcaa agaagcagaa cagtttccgc agtttagatg
    37081 ggtgagtttg gaggagttga gatgacatat ttaaaaaaat tatttgaagc cgttaaattt
    37141 ctcaactcta tggaaaaaca gaacaaaaaa ataatgatta ctttccataa taaaaaagtt
    37201 catttttacg agttagaaga aattacagag tcttcgagtg gtactgttga agctaaagcg
    37261 aaaatgctta gtttatgggg aaagattaaa ttatgaaaga aaaaacaatt tttatatcaa
    37321 aaaaatatgc aaatgacttt aacaatgaca aatataattt gtcctctggc tattatttta
    37381 gaagtggtga aaaacatgat attgctattg ttaaatatgg tgaaaaagat tatttaaaaa
    37441 atactgattt agcatatgtt gtatgcgata agatcgttga cgcagactct ataagcttcg
    37501 tttatcatgg tgaatatgaa acttggcatt ttaaactatt aaacacagaa gcaaattaaa
    37561 gtcccacgca agcgcctaag agcctgcaat agctctgtgg gtctacgagc tggaatactc
    37621 gttaaactta ccctggaagc tttctgtaag tattcagctg cgtagcgtgg aataatcgtt
    37681 acgtagttat agagcgaaat ttttagaaag ggaaatatcc tccgacattt ttttcataaa
    37741 aatctaaagt ctgttatcgc tcacagatga ttatacaagc gtaatgctgc aaataaagtg
    37801 ctgacgcaaa actaaaaatt taatactcga catttaacaa cacaaaaaaa cagtcagcag
    37861 aaaaaggaaa ggagaacaat aaaaaagcgc tcgtgaaagc gccattcggt atatattcgt
    37921 acaactatta tatcatacga ggagctttca tgacgttttt tccagaaatt aatatacaaa
    37981 agactaaatc aaacgctaag cgaaaactaa gagagtatcc acgctggcgt aggatcgcta
    38041 atgatgtaga tactcaaaaa gtgacagcca cttattcctt tgagcctaga caatcacacg
    38101 gaactcctag taagccagtt gaacgcttag cactcaaccg tgtgtcagct gaacaagaat
    38161 tagaagcaat tgagcaatca gttagtatga tattggagcc agaaaagcgt aggattttgt
    38221 atgacaaata cctatctcct tacaaaaatg cagataaggt tatttataca gaattatgta
    38281 tgtcagagag tttttattat gacacgctag atgctgcatt attagctttt gcagagcttt
    38341 atagggaggg ttctttgatt gtagagcaag gagtttttga ctagttttta tacagtaata
    38401 caatagttta tacataaaaa tatgtgttaa tatagtatta tcaaaatagc aagaagagat
    38461 aatcatttac caacaggcta tttatttagt cgtcaacttt aactactatc gaacttgcta
    38521 ttttgtagct aaaaggcgag atagggtgtt gagacgtagc tcagttgggg gagcgatatg
    38581 actataaagg gtctggaacg tacgcaggtt cgaatcctgc cgtctcaata gtggttattt
    38641 cagccactag agcaatacag cgggcgtggg acatggatcg gagttttaac cgtttttttg
    38701 tgtagaccta gtggtattaa tcacgttcga ttcgtgatgg gtctataggc ttactttaaa
    38761 aataagcact ggtatctcta cggggacctt tgcgccaagt aagactaaac cgttggaaca
    38821 tgaaccgtga ttggaaaacg gtagaggtag cgccttgata attggattgt cgacggtctg
    38881 attatatgtg tcggttcgat tccgactgtt cctatagcgt gtattggcaa caaggttcaa
    38941 ttccttgtga tgtaagtagc taaatatcta ggcgaagtgg ttcgaatcca ctcacacgct
    39001 ttactcggtc atcacattgt ggtggctttt tattgtggag aaaaaaatgc ttaaattaga
    39061 cgaaaaaaaa ataagaaaag gtaagccatt tggacttccg taccaaggta gcaaaaagaa
    39121 aatcagcaaa aagatagttg aaataatcaa gcaaaatttt ggcacagaca aaaaaatata
    39181 tgacattttt ggtggaggag gtgcaataac tgcagagtgt atgctaaata atttagaagt
    39241 gcattacaac gatttaaaca atgatgttac tgatatgttt aaaagagtta ttactcaaga
    39301 tagagagtgg ttaaaaacac taatcatcag ccgagaagaa tttttaaaaa taaaaaccaa
    39361 aacaaacaaa acagttgacg acaatattaa attgcttgtt aacagttttg ggaacaactc
    39421 aataagtttt ttgtataaca tagaattgtc agatataaaa tataaattag cggttgagat
    39481 tattaataag catgatgtat ttagtggcta taagcaaaca gacacataca aagagacttg
    39541 tcttttatgc gaggaagaaa agcttggtaa aatcggaaac ttacagcgat tacagcaact
    39601 agagcaattg caacgattac aacagttaga acagttagaa cgattagaag cgacaaattt
    39661 agattataaa gcattctcga atgttgatgg cgctattttt tatcttgatc caccgtatga
    39721 aatgacgagc cagcattctt acattggtga tacatttgac agtcaattat tctacgactg
    39781 ggcgtacgaa atgagcaagg aaaatattgt tattgtttct agttacgaaa tatcagatga
    39841 tagatttaag tgtgtgtatg agtttaagac agctagaagc acaatgcaag gaggaagtgc
    39901 ttgtggtaaa accgaaaagt tgtttatggt agtgtagctg actgttgagt ctatttttat
    39961 tatgcaaaaa agaaccacaa cagcggctct tatgcttgta attttaattc aagtgcttca
    40021 gtaaggactt gagaaaagtt gaggttttta tcttcggctg cgttgttcaa ccactcagga
    40081 atagtcacgt ttttgcgtac cttcttagag tgatattttt tcatgtaggc aatcatatca
    40141 atgccaatta aagcaatatc agaatcagga tactgttctt ttaaatcaga aacggagcta
    40201 gcctttggat aatcagtata gtcttcaaga gcaaatccta agacttcgac agccatttca
    40261 taggcgtgct ttaagtcgtt gccgaaagta atagcttcag gaacatctgg aaaacgaacg
    40321 ccaattgaat tagtttcgtt gtcgtgtgta aaaacagctg gatagattaa catagttttt
    40381 cctttctagg taaacaataa ccaagatggt cagagcttat ttcagccctg ccttcttgag
    40441 aatatcgtct tcggtgcctt ttggcatatc acctttgtgc atgggcacga ttgtgatgtt
    40501 gcctgtttca aagtttttgt actttgcgtg actaccgttt tgactgactt ttacaaagcc
    40561 gttctttttc aagagtttga tgatttctct tgcggttctt ggcatattgc ttacctcact
    40621 ttctatactt atattataca cacataagca ataattgtca agcaaaacac gcataaaata
    40681 cttatttttt aaaaacggag gaggtggtgg aaaataggta aattaacact aaaacaacaa
    40741 aaatttatag atgagtacat catctgcggg aatgcgactg atgcagcgat aaaagcgggg
    40801 tatagtaaaa aaaccgctgg tcaaattggc gagcaaaact tgaaaaaact tgaaataaag
    40861 caagctatac agagtcgtat gaaagtctta gaaaagagtt cgatagcaac agctgatgaa
    40921 gttcttagag tgtttacaca aattcttaga caggaattaa ctgaagaagt aacggaactt
    40981 aatcagatta ctggtgaatt tgtaacgata gagaagcaac catcaatagc agaggttata
    41041 aaagcaggaa gcgaattgat gaaacgttac ccaacaaacc tagaactcaa aaagatcaac
    41101 cttgaaattg agaagttgaa gtctcaaatt ggcggagatg aaggacaaga tgagaaaata
    41161 gccaatttct tgaatatggt caaaggagct atttcagatg gagttgagta agttatacac
    41221 aagaaagcaa ttgcaagttc ttgattacat ttggaataac gattggttta tctgcggact
    41281 tcatggagca aagcgtgctg gaaaaactgt tgttaacaac gatacttttg taacagagtt
    41341 atatcgtgtt cgtaagattg cagatgactt aaaaatagat gaacctatct atattttagc
    41401 tgggacatca tctacttcta tccaaaacaa cgtgttacag gagctttata ataaatatgg
    41461 ctttgagcct aaatatgaca aacatggatc ttttacattt tgtggcgtta aggttgtgca
    41521 agtatatacg gggtctatta gtgggttaaa aagagctaga ggttttacat catttggtgc
    41581 atatgttaac gaagcttcac ttgctaacga gacagtgttc aaagaaatca tttctaggtg
    41641 ttctggtgag ggtgcaagga tagtttggga tagtaaccca gataacccta atcattggct
    41701 caaaacggat tatatcgact ctgacgatga tatgattatt gattttagtt ttaagttaga
    41761 tgataacact tttctatcta agcgttacat tgagtcgata aaaagcgcaa cgcctagtgg
    41821 aaaattctat gaccgtgaca ttttaggtaa atggacggtc gcagagggtg ctatttattc
    41881 tgattatgat aaaaacgtcc acgaagtcga agaattgcca aaaatgacac gcttttttgc
    41941 cggtgtcgac tggggatatg atcactatgg ttcaattgtg attatcggag aggacaataa
    42001 tggaaattat tacttagttg atggtattgc agagcaatat aaagaaatag attggtgggt
    42061 aactagagcc aaagagttta tacaaatcta tgggaatgtt acattctggg cagatagtgc
    42121 gagaccagaa cacgttgctc gctttaaaag agagcgtatt aaagcaagaa atggacgtaa
    42181 agaagttgtt gcaggcattg aatatatagc gaaatcattc aaggataaaa ggttatttat
    42241 caagcgaggg tgtattcctc gtttttttga tgagatatat caatacaaat ggaaacctaa
    42301 cagcacaaaa gacgaaccac aaaaagaata cgatgatgtg ctagatgcca tcagatatgc
    42361 tctttattca cagcataaag aagatagcat aagtaaaacc aacaatttca atgtacttta
    42421 tcaaggtctt aaaaactaga taggagaaac aatggcacac atagaagatt ttattgactc
    42481 aactggggaa cataaacttt tagagttgcg ttttcatcgt gagtcaagaa tgaggtatca
    42541 gataagcgat ataagcgctt tgtttgatga caactataaa atattacttg aatacttaaa
    42601 acaccacgag aacattcaaa aaccacgtat acaagagttg ttagattatg cagaaggaaa
    42661 caatcacgag gtatctaagt ctggtcgtag gcaagatgag gacatggctg atgttcgtgc
    42721 catacataat tatggcaagt atatatcaac atttaaacag ggatatcttg ttggtaatcc
    42781 aatacgtgtt gagtatgacg atgaagttaa caacgaactt ttaaaagagt tagcaaaaaa
    42841 gaacaatttc caccaattaa atagacaact tataaaagat ttgtctaaag ttggtcgtgc
    42901 ttacgaactc gcttatcgta gtgcagatga taaaacaaaa gtgataagac tagacccgag
    42961 agagactttt attatctata aaaatgacgt tgataaagat agtcttattg gtgtgcgata
    43021 ctacaacaaa agtcaaatag ataaaacaga caagacggta gaagtttata caagttcgga
    43081 agttatcttt tttgaattta atggagaatt aacagagaca gatagacaac cgcacgcttt
    43141 tggtgctgtt cctatcacgg aatatcttaa tacagatgac ggtttaggcg attacgaaac
    43201 agaattatct ttgattgatt tgtacgactc tgcgcagtca gacacagcca actacatgca
    43261 agacttgtca gacgctattc tagcgattat tggtcgtgtg tcgtttcctg aatacgtaga
    43321 tacgcaagaa aaagccatta catatttgag agctatgcga aaagctagat tgttaaatct
    43381 agaaccacct gttgatagcg aaggacgtga aggttctgtt gacgctaagt atttgtataa
    43441 aaaatatgac gtacagggaa ctgaagcata taaaaacaga atcgctgagg atatccacag
    43501 atttactaat acgccagata tgacagacaa taaatttgct ggtaatcagt ccggagaagc
    43561 gctcaagtgg aaagtttttg gactggatca agaacgtgtg gatttacaag cgttatttga
    43621 gcagtcgttg aaacgtagat ataagcttat tgctcatgtt agtgagtttt taagtgaaat
    43681 taaagatttt gatatcagca agctaaaaat aatctttact ccaaacttac ctaaatcaga
    43741 ccaagaaaaa ataaatgatt ttaaagcgct tggtggagaa ctatccaaca aaactaagat
    43801 gtttgtcact ggtattgttg atgatccaga cgaagaagaa gataaaatca tgcaagaatc
    43861 gcagtcgggc agtttgctag ctcaaaaact agaagctcaa acacgtatgt cagacaagga
    43921 gttagctcat ggacatgcac actaaggaag gcaaaagtta ttggcgcaaa cgtgtaaaaa
    43981 aagagatgga agccaaagac aaaaaagatg tcagtttagg aaaatctatg aaacaaatac
    44041 acgactatca cttccgtgag atagaaaaag aaatagagtc tttttatcaa cgttatgcag
    44101 ataaagaagc gatagactta aagcttgcta aaaaggctgt ttctgacgtt gatataaacg
    44161 cttatcagaa aaaggcaaaa gaattagttg caagggctaa cgaaatgcgc aaagagggaa
    44221 ttaaagtcac taaagctaat tttacgcatc aagaaaatat agacatggct gtttataact
    44281 taaagatgaa agttaacgca ttagagcttt tgcaattaaa tattgattta gcgatgcaaa
    44341 acttatcgga agatgaatac aaagcgacaa aaagattcct tgaagatggt tttgaagaag
    44401 agttgaaatt tcagtcgggc ttacttggaa catctgtttc tagtcaaaat gacattaaaa
    44461 atctagctaa agcaacaata aacgcaaatt tcaaaggcgc aacgtggagt gaaaacattt
    44521 ggcaaagaca agatgacctt agaaaaatag taaaagagga agtttataaa gctgtaacaa
    44581 aaggcgataa cgctattaag ttgtctaata agctaaaaaa agagtttgag gtatcagaca
    44641 gctacgctag acgtctagcg attacagaac atgcaagggt acaaatggaa gttagcaaaa
    44701 tatcaataga agataacgga tttaacggct ttgagatatt acccgagccc tccgcctgtt
    44761 ctatttgtaa aggaatagca agtgacggtc cgtatcctat ggaaaaatgg gatacaggca
    44821 atacagcgcc accatttcac ccgcattgtc gttgtgcagt cgttggtgaa gatatcgaac
    44881 ataagaaagg taaaaaatga acaaacgcat taagaaaaaa cgcaaactag aatactatat
    44941 cgcttcactc gtcgcagaaa acgttatgtt ttcaaaggaa ttaatcaaac aacatgaacg
    45001 gattgaacaa cttgaaaaaa ttgtagagca taacgcacaa gcgactaaca atgaacttag
    45061 tcgcatcaaa aaacactcaa agaaaaaatg gaaaaaataa gtagtaacaa aaccagttga
    45121 ggctggtttt tattatgccc aaaacgtgct tacggctata aactgtgcaa gatttaatag
    45181 tcagacatga ctttaaacag gaggcgcctc atggcagatt tagttaacaa tggtgtagta
    45241 gacgaagtaa cacaagagga agtcgaaact caagaagaag ttaaagcgga agctacatca
    45301 gagaagactt ttacacaagc ggaagtaact gaaatgatac aacagaatgt taatcgtgca
    45361 gttgcaaaag ctcacaaaga cgctcaggag caatttaaag cagagcaaga cgaagctaaa
    45421 aaattagcaa agatgaacag cgaagaaaaa gctaactacg aaacacagaa attgttagaa
    45481 gagttgcaac aactaaaaga tgataagaca cgcaacgaac taacagcagt tgctcgtaaa
    45541 atgttttcag aggctgacat taatgttgat gatgatgttc ttagtcttgc agtaacttta
    45601 gatgcagagc aaacaaaagc aaatgtcact aagctagcta gcgcatttgc taaagcaatc
    45661 gctgatgatc gcaaatcatt ggcacgacaa gccacccctt caattgggag tggttctatt
    45721 acaactcaat caaattttgg tgcaagctta gcagaacgag ctggaaaagt taacaccaaa
    45781 ctattttagg aggaagaaat gaataaacgt acagtaaaaa catcaaaaga gattttacat
    45841 aacttgccat acgaagcaat ttctgtaact ttagatgcga atgcatttgg taaaggattg
    45901 gcatccgctg gaacagtttt agcaggtgat ggggcatctg tttttaaaga ccgcaccaaa
    45961 aaagtaaaac cagttcgaaa tgctgaagtt tctggtgccg atcatattga tggcattttg
    46021 ttaacggatg tagatctgtc taaaggtgac gctacgggtg cacttgttta tcgtggtaca
    46081 gtaaactctg ataagttagt tgaccaagag ttagctaaag acacatcaac actagctact
    46141 aaattaccac acattgtatt tgtaaaagga ggaactaaat aatggcatta atttatgacg
    46201 ttgtaacatc tgctaacatc aaaggatttt atgataaaca acaagcaaat gttgacttga
    46261 ctttgggaga aaaagctttt ccatctaaac aacaacttgg tcttaagtta tcatttatca
    46321 aaggagcagc tggtaaacca gttagtatca aagcggcagc gtttgacact aaagttccac
    46381 ttcgtgaccg cattgctgta gtcttattag acgaagaaat gccttacttt aaagaaggta
    46441 tgcttgtaaa agaggctgac cgtcaacaac ttaacgtttt agcgcaaact aaaaatcaag
    46501 aacttattga cacagtgtta tcaacaatct ttaacgatga aactactcta atcgctggtg
    46561 ctaaagcacg tcttgaagct atgcgtatgg aagtgttgtc aagtggtaaa atccacatca
    46621 attcaaatgg tgttatgaaa gatattgatt atggattaac tggaactcaa acgactaaga
    46681 gtgaacaaaa atggtcagaa aaagacaccg ctaaccctct tgctgatatc gagaaagcta
    46741 ttgaaacagt aacagagcga ggtcacgttc ctgaagccat cgtcttaaac tcaaaaactt
    46801 ttggctatat caaaaacgca aaagcaaccg taaaagcaat taaaccactt gcaccggaag
    46861 gctcaattgt tactaaagca gaattaaaat cttatctttc tgaagaattg ggattaaata
    46921 tcttacttaa agatggtgtg tttgttaatg acgcaggtga aagcaagaag tatttccctg
    46981 atggcgtagt tacacttgta cctaatggaa atcttggcta tacagtattc gggacaactc
    47041 cagagcagtc tgaccttatg ggtggccaag caactgatgc acaggtatct cttgtagaga
    47101 caggtattgc tgttacaact actaagacta ctgatcctgt taacgtacag actaaggttt
    47161 ctatgattgc tctaccatca ttcgagcgct tagatgaagt acagattgta acaagttcgg
    47221 aagtatcatt ataaaaggag gtaatcgtgg ctctagtaat tgaagctttt agagataaag
    47281 agactggttt tatttataaa gtcggtgaag agtataacgg tgctagagtt gagtttttga
    47341 ctggtaaagg tgttttaaaa gctactgata cacagtcaac aaactttagc aatttaaaag
    47401 ttgacgagct aaagcgtgga cttgatgaac taggtgttaa ttacgattct aaagctaaaa
    47461 aagcggaatt gctagagctt ttagaatctc acaccgatta atttttggag gtgtttatgg
    47521 aaaagataaa cacgcaaaca atcattaata atgtaaagct tgatttagat atcaacgata
    47581 cattacaaga caaactattg gaaatgttgc taaaaaggat tactgaccac ttttcagcag
    47641 agtatggcac taatgagata gatagtgcct tttcgtttgt ccttgaagat tgtttgattg
    47701 ctcgctataa caggcgaggt gctgaacggg ctaagtctga gtccgtggaa ggaagagcta
    47761 tcacttatta cgacttttta aacgagtttg aaccgtatga tttaatgatt aaaagtaagc
    47821 ttaatatttc aaatcaaaaa tctaaaaaag gtggacttta ttttttatga gatataacga
    47881 tagagttact cttttaatta aagctaacgg tgaacctcga tatgatccaa ctcttgggaa
    47941 acgtgttgga ggagaagtta aaaaagatgt cgttccgtgt aacatctcgg agcaaggtat
    48001 agaactatct aatctattag acgagaagtt agaccttgat agacgcattg taaggctacg
    48061 tcacagcgtt aagaaggtgg atagggtttt aatcaaagat aaagcttatc gtgttgtaac
    48121 gagcagaaat aaagctttat acgttgagga gattgttaat gattaactta acattggaag
    48181 gggaacatga gttgctatct gcgttaaaaa aagaagttac ttttgaaaac aagcgcaaag
    48241 ctgtcagaaa aaacggtcgt aagctacata aaaaagctgt taaaaacgca aatttcaacg
    48301 gtcattataa aaaaagaaaa ggcgaggaat atgtatttgt taaacctaca ggagctacta
    48361 gaaggtctat taaattagag tttagtaatc aaagtacagt tgctaaagtg aaggctggaa
    48421 caaattattc cggttacttg gaaacaggta cacgattaat ggaggagcaa ccgtttatga
    48481 agccagctct tgatagtgtc attgacaact tcattaaaga tttagcgagg gttgaatgat
    48541 gaaacaaccg gatcaacaat tgtttgatga gatttttaaa agactcactg atataggatt
    48601 gactgtttac gatttccttc caccgttggg tacaaaatac ccttttgttg tgatgggaga
    48661 cactcatata attcctatgg ctacaaaaac gcagttaata ggtaagtgct caacgactat
    48721 caatgtttgg ggagatggaa caaatagaaa agtagtaagc gatatgattg ctcaaatcat
    48781 gttggaagtc agtaagatta gccaaattga tggtaacaaa tgggctatgg taatgaacga
    48841 aagcgatact caaatcttaa aagataacag tacaaacgaa aatctttatc atggcatttt
    48901 aaatatttat tttaattttg tataggagga aatatggtca caagttcacc gatttatggg
    48961 aaagataata ttttgatgtt ccgtgtgctt ggagataagg cagctgcggc taaattgtct
    49021 tttcagacag aacataaatg gaaatatagt agaaaaacag atgctaaaat cacaaaagat
    49081 ggtgctatca attctgacaa agggcttgag gtaactttgg aaatcaaagg cgtggcaagt
    49141 cgtgatgaat tgaatacgac actaaagaat gctgttttag aaggtaaaca aattgaagtt
    49201 tgggatattg acttgaatag caataacgat tcagacggga aatatgatgc agaatatgct
    49261 attgggcgat tgggatcttg ggaagtccct tccaatgttg aggaattttc tgaaatttcc
    49321 acagaaatgg ctatcgatgg caaaccagtt aaaggtaaag ccactcttac aaaagaacag
    49381 attaaagcta ttcaatatgt atttaaagat gtaacaaaaa ttgatgaatc ttccgtttct
    49441 agtccgggtt ctgttcatag cagataacat agattctaaa tagcaaaata acactagggc
    49501 ttttagccct ttttattttt taaggagcaa aaacaaaaaa tgaaagaaat tacaatttca
    49561 ggaaaaaatt acccattaaa ctttggcttt gactttattc gagaaatgga taagcgcttt
    49621 tttgttgata acaatggctt taagtttggt acaggagttc aaactgcagt tatgcagcta
    49681 gctgcaaaaa atccgcttat tttagaagat attattttat cagctacaca cacagaatac
    49741 tcaatcccta aaaaagaaga tattgaaaag tgggctatag aacaagcaga aaatggaaaa
    49801 ctgggagaag ttttagatga ttttttaacg atcttgaaga aagcacctct tttgaaaaca
    49861 caagtaagcc caatcttaaa agctttagag gagggttaaa tcaaaaagat ataacaagtt
    49921 cttctcttga aacctacaac gagattatta gcaacatttt tgggttgttg gatgtgaaag
    49981 actttaatgt tgctcgtcgt atgacagtaa aagaatacaa tttaaggatt aaaggttatc
    50041 ttttaaaacg tttagaatct gaaagagata tttacttaag tgcttttgtt aatcgcaacg
    50101 ttaaagctgc caattctaaa ggcgaatatg tttaccgtga atttgaagag ttttacgatt
    50161 atgagaaacg gaaaaagcaa gtattgtcag catcggttga taataccata aatcctaaaa
    50221 tcattgacag agctaataga gttaaggaat taagagagag aggaggttta aatgtctagt
    50281 agagcataca cagtacaagc gattttaaaa gcaacagata ctaattttac aagcactatg
    50341 aacaaagttc aatctgctgc acaagctact attgataaaa taaaatcaat aaaagatagc
    50401 aacatatcta cgcttgggaa aatcggcgaa tatacgacta tggtaggtca agggatgcaa
    50461 agtgttgggc gtagcctttc aaaatacgtc acacttccaa tagttgggtt aggagtcgct
    50521 gctgcaaaaa catttggaga ttttgaatct caaatgaaca gagtaaaagc aatctctgga
    50581 gctaccgggg cagattttga aaagttaaga aaacaagcta ttgatcttgg ggcatcgtct
    50641 gtttttagtg ctaaggaagt tgcacaaggt caagaaatga tggcatcagc aggatttaac
    50701 gcaaatcaaa tcttagcagc ttcacccgga gtaatgtctt tggctgcagc atctggtgga
    50761 gatctagctc ttgcctcaga agcggcagcg actgcggtaa atatgtttag cttgaacgca
    50821 agtgaagcca ctcatgtagc cgatgtgttc gcaaaagctg cagcagatac aaactcagag
    50881 gttgcagaca tggcggaagc gttaaaatac gcaggaccag ttgcaggagc tatgggaatt
    50941 tctatggaag aaaccgcagc agcaataggt attatgtcaa acgctggtat aaaaggctct
    51001 caagctggta caactcttag aacagctatc accagattag caaaaccgac agaccaaatg
    51061 caagctgtta ttgatggtct cggattgtct ttttttgata cgaatgggaa gatgcggtct
    51121 ttgacagaga taacaggaca actaagagag aaaatgtctg gattgacaga tcaacaaaaa
    51181 tctgcagctt tatcaatttt atttggtaaa gagtctctgt cagggatgct tgctttaatt
    51241 aacgctactc caggcgagtt gtctaaatta accgaagggt tgaaaaattc aaaaggtgct
    51301 gctgacaaaa tggcaaacac tatgaatagc ggtcttaaag gagcaattga acagctcaaa
    51361 ggttctttag aaacagccgg cattacaatc ggtggtatat taaatccatt gttgcaagga
    51421 gttattggta aaatccaagc agtcatagat tggttcaata agttatctcc tgctgggcaa
    51481 aagctggcag ttattattgg aggtattggc gcagctcttg ggcctctttt ggtcattttt
    51541 ggaacagtaa ttatttttat tggtcaagta actgcttctt tagaaggagt ttctacagcg
    51601 ttcacggcaa tagcaggaat cttttcaata atttcaggac ctgtaatttt agttgttgca
    51661 gcaatcggag catttatagc tgcacttgta gtcgcttgga atacatctga gacatttaga
    51721 aatacaatta tttctggttg gaaatcaatg gttgatgcag tcaccccgct aatcgaaacg
    51781 ttgaaacaac taatattatc aattttcact ttcatacagg ctaacagcgc tacttttatt
    51841 ggcggtttaa agttagcttg gagtgcgctt gttgaagctc taagtggttt gtttctggtt
    51901 ataacaggcg tggtacaagt tggcatgtct gtcatcaaca caactataaa agtgatatta
    51961 gcaattataa atggtgattg gaatgcagcg tggactgcta tcaaatctgg agtaggagca
    52021 atttgggaag gtataaaaaa cataataatt gggggtctga cgtttttagc tagtttattt
    52081 gtaagtatgc tgggtgtttt cgtatcagtt ttttctgctg gatggagtgc tataaaaggt
    52141 atattttctg tgacgctaaa atttttggtt aacgtagtag ctgctggaat gagtgctatt
    52201 ggaaacgaga tatcatctaa aattaataca gcaaaaagtg tcgctatact agcatttaat
    52261 gcgatgaaaa acggtatttc aacagctatt aatgctgcga aagaagtcgt ttctagtgta
    52321 gttaaccgta taaaaaacat tcttaattcc cttgctaata ttaacttagg agcagctggt
    52381 agagctataa tggatggatt tttaaatgga ttgacctcag cttttgaaaa agtcaaaaat
    52441 tttgtcggtg gtatcgctgg atggattcaa gagcataaag ggccaataag ttacgataga
    52501 acattactta ttccagcggg tagagctata atgggaggtt ttaataaatc tcttacagat
    52561 agctttgaac cagtcaagaa aaatgtttct agtatggcta gtcgtatttc tgaggagttt
    52621 caaaacggat tgaataaact taaagatatc aacatatcgg aaattacgga aggtttacaa
    52681 agtagttttg acgtgactca ttatgcaagt tttggcggac taaatgactc agcaagaaaa
    52741 ttagaagatg atagatttgt cgcattgaaa gatgcggttt tagctattag agattttgct
    52801 aatagagacg ttgtagtgac tgtgaatggt aaagaattag ctcgaacagc tggagacaac
    52861 ttcacagaat accaaaaaca aaaagaaata atgaataata gaatgaaagg tttgatataa
    52921 ttggcaaatt ttagttataa aggcgttgat ttatcgcctt ttttgaattg tttaaacata
    52981 gtgaggacta taggtaataa taggtctatt gctaccagaa aaataaatga attaggagaa
    53041 gccattcaat cggttagttt tggagctaaa actatttttg ttactgttag ttttaaaact
    53101 aaagaaatcg gagcaagtaa atttgtagac acgacagaac cgtcgactta tagttacgaa
    53161 aatttaaata aattaagaga aaaaatagct ggtattttgc acagtaagac gacctttaaa
    53221 cttacattac cagatgagcc tgacagatat tatatggctg ttccaaaagg tgatattgac
    53281 ttaaaaggaa tatctgattg gtacgacgaa acggttattg agttttacat accagacggc
    53341 gttgcacatt cgactactta caaaaagttt ttagattaca cgcgagatgg aaataaacta
    53401 acctttaaat tgcaaaacga aggtaacaca aatgcgttgc caattatcaa aataaaacac
    53461 aactccgaaa acggctatat tggtattgca aacgaaacag gtgcttttgc gcttggatca
    53521 tcagaagaag aagacgggac tatcgtgcat cgtaacgaag tcctttttga ttactcaaaa
    53581 gcgatagcga aagctttgga gggtgcgcca aacgtcgcaa aacttaatca catgccacct
    53641 tctttcgata cagaacttaa acgtatgcgt attgataaca tcttaggttc tggcaaaggc
    53701 ggtgaatatg ttgttattgg aaatagaggt actactcctg gctacacaga gcacgttggg
    53761 actcgaacgt ttattatcaa tcctgattca aatggggaat atactctcaa tgaacatttg
    53821 tggtggcaac agatttttat tgctactgcg caggatcaaa aaggtttttt aaagctttgt
    53881 gtaacaggaa tcgatgatga aggaaatgac gagtttctgt atggaatcga aacttacaaa
    53941 cgaaaaaatg gttttgaagc agaatacaat ttctttgctc tggatgatga cggtgttggt
    54001 tggagatttt ataagcagtt tgaatttcag gcagatagaa attatcacaa tcctttttcg
    54061 atggatagaa gcagagctgt tgagattttc agagaagaag ataagtttcg tatttacttt
    54121 aacggtgcgc atcatcatgc aactgttcca tctcttaaag gaaaaaaatc tcgcaagata
    54181 catcttgcaa tggggacatg tagtgatagc tctaaatata tcaactacaa cctgtttgaa
    54241 aaagtcaatt ttgaaaaaat gggagtgtct cattacaaca atatcgtcaa taaatatcaa
    54301 ccaggtgacg aagttattat taattttgaa aatgatacag tcaaaactaa agatattgac
    54361 tccctgcagg atatggtttt aggctctcaa ccaatatcta ttccaccggg tgagtctgaa
    54421 ctcgtcatca atgtgtctaa attttcttca acagaccctg acatcgaact attgttagaa
    54481 gagaggtggt tgtaataact ctagtaatac acgacgcaaa actacatcca gttttgcttt
    54541 tagacaatga gcgacaagga gcacttaatt attatgatga tttgtggact agacagctca
    54601 caactggttc gtcagcattt gagttttctg tttataaaaa atcgctgttg ggtgataatc
    54661 cacttaatca caaatatcac gcactaaacg atcaagcatt tgtttctttt gtacacaaag
    54721 ataaagtaca attgtttaac atcatgcgag tcgaggaaac agagacaaca atacattgct
    54781 attgcgaaaa tcttaattta gagttactaa acgagtattg caacgcatat aaagcaacta
    54841 aagcaatgtc atttgaagag tatcttgtgc agtttgatat tttaaattgg ggtgctttga
    54901 caattggcac aaacgaagtt aaggacaaaa aactgacatt ggaatggact ggtcaagaca
    54961 ctaagttagc tcgtattttg tcaattgcta ataattttga tgcagaaatc gaatttgaaa
    55021 ctcaattaca caacaatcac acgtttaaag cgtttattgt aaatgtgtac aaggaatacg
    55081 aagagggcgt gtcctatggc gtaggtcgtg accgcagcga catagtgttg agatatcaaa
    55141 aaaatgtaac tggtatcact aaaaaattag acaagcgcca gatttacaac gccatacgcc
    55201 cgtatggcaa aaagacagtc aaaggcgagc gcgttatttc taatcctgta actcgcaaag
    55261 tcactaaaac agttgggtca aatcgtacat atttaggcgg agacctcaaa tattatggtc
    55321 atacaatcaa aaaagctaac gtacaatcta ttattaacta cgcggtgcag tataatattt
    55381 tgccgagtgg aatcatatgt caactgtact tagaaagtct ttggggtgat tcagcagttg
    55441 gtaaacgtga caataactgg gcaggtataa gcggcggagc acagacacgc cctagtggag
    55501 taaaagtcac tactggaatg gctcgtcctc ccagcgaggg cggaacgtac atgcactatg
    55561 ctagtgtaga cgacttttta aaagactaca cttatctttt agcaaaacaa gggatttata
    55621 atgtcgtcgg caaaaagaat atagcagact atacaaaagg gctttttaga gctggtggag
    55681 ctaaatatga ctatgcagca gcaggatatc aaagctacac aaatttgatg actaatatcc
    55741 gaaatggtat caataaagta actggaaata tcctcaatac gattgataag ctgtggcaaa
    55801 caccagtaca gcctataaca gccgtaaacg tagctagaag agctactaag acaatgcaag
    55861 cactaaatga agctactaga cttaaaggtc gcagaatcgg ctcaggacag tgttatgctt
    55921 tgtctggttg gtacgctaag aagttagacg gcgcttggat tgacagctcg gttggtggta
    55981 ttagaggtcg tatcggaggc ggtatggctg ctgccttaat cggcactgat tataactggg
    56041 gtgcttatgg ttggaagcta gacaggtcgc ctaatgctgg caacttgcaa gctggcggta
    56101 tctataatgt taaagcaaat tttggtgctc cattttatac aacacaatgg gggcacacag
    56161 ggattatcaa gagtgtgtct aaaacaagag tcactgtctt agagcagaat tacgctggac
    56221 gcatgtatgt catggaaaac tcgtatgaga ttaacgcttt tgctagagga ttgcagacag
    56281 tatgttatcc acgtgaaata gcgcaaggaa tggctgttaa cggtgcaaca acacagcaag
    56341 taagcggtgg aacacaaata tcatacgagg aagtcgtgca agaagcacag acagaatcat
    56401 acgaagaaga acaaatcatc tacattgaca actcaatcta caaagagtgg aaagacgaaa
    56461 acggtaaagt agagtactat ctcaaaaatg gatttttgta cgcaccttta tcaagagacc
    56521 gttatccatc tgttttaaca ggtaacgaga cacgagataa ctggatacga aaagacatgg
    56581 aagttgagac tgatagtcag gatgtcttga tatcaactgc tttaaaagat ttaaaagcac
    56641 acgcttatcc agctgtcact tatgaagtcg atggatatgt tgatttagaa cttggtgatg
    56701 ttgtgcgaat acaggacgac ggatacgagc caccgctaat tctcacagcg agggttattg
    56761 agcaagaaat atcaataaca aatcccagct ctaacaaaac taaattcagc aattttgtcg
    56821 aaaaagaaag tcagttagct tccgacttaa ttagtgatat gttgcgtcta tacgatgagt
    56881 caattccata cgatatacaa ctagcgactt caaacggagt tgcttttaaa aatggggttg
    56941 gtgagtctgt attaacgcct aacctgcaaa aaaatgggaa agattacgat gctatttatt
    57001 tttataaaaa tggcgactca ctgattgaga taggtccttc gctaacagtt aaagcaagtg
    57061 actttaacca tgttttaaac ataacagtcg aagcttatgt taacgaggaa cttgtagcaa
    57121 gtacgcaaat atcctttaca gatactgagg atggagaaaa aggcgatgat ggtgctacat
    57181 catggacagc gtgggccaat tcgaaagatg gaaaagttga ctttagtatt actgaagcta
    57241 aaaatagaag atttatcggt acttatactg gattaacgca atcaacaaat tatcttgact
    57301 acaagtggat tgatatgtct gctaatgttg tcattggtac tcaaaattta cttgatggta
    57361 caaaatcatt ttctggaagt tggtttaccg aaggtacaat atttgagact acaaaaatca
    57421 gcgaatatcc atttgaattt aagaaatgga agtctggaaa taaggttagt cacactatcg
    57481 agtttgatgt taaagctggt gtaacataca cttttacagc tgctatagca agagaaaatg
    57541 ctggaagatt gtacttctat ttgtatgact tgtttgcaaa ccatatcaca agtaacacac
    57601 ctcgtgagac gataattgaa aatgtcacta cagatatcca gatgtttaaa gttacatttg
    57661 taccgctcag agacggtaaa ataaaaccac gctttgccat gcttgccagt gatgcaggtt
    57721 ggtttatgac tggtggatat atgcttgtca aaggtaataa atctggagat tggcaagagt
    57781 cagaagctga taaagcaagt aatcttgatt caaaagctga tcaagaatta acccgagcac
    57841 aaattctagc tcttgaagaa agaactgcta tagcaagaga aaatgcaatt gctgaggcta
    57901 tgcagcatac actcagcgaa gttgaaacta agtggaagct ttggtatgac ttaaatacga
    57961 tagacgaaaa gcaaaaagtt gcaaacgaca ttgctcaatt gtttgatcgt acaactgagt
    58021 ttaaacaact tttaggtgag gcaagtgcaa aatttagctt tatcaacaac gagacattga
    58081 ttggtgaaga ggggattgcc attggtgaca aagatggcaa agctaagtta ttcctatcaa
    58141 atgacagcat ctcctttgtt actaatggtg ttgctcaaat gacactaaca ggtgatacct
    58201 taacgataaa gaatggtctg tttacagagc gtatacagat tggaaatttt gttgaagaag
    58261 tctatgacag aaatccatta tttaatgtaa tcagagcgat taggaatagt taggaggtga
    58321 gaaatgggaa catcaacatt tagtggaact tggggtaaca atctaacatt agatgtacga
    58381 acaagctata cacaaaattt agttggtaat tacagtacgg tcacagttaa agtctacgaa
    58441 aaaattagct cctatggtta tattgactac cctggcgaac gtacaatgac aatagttgtt
    58501 gatggcaaat cttataatga gagtgtcaac gtagacatta attatggtca gacaaaagaa
    58561 ttaggtacat atagctaccg tataaatcat aactcttatg gatcaaaacc aaaatttaat
    58621 gttacggtaa cgctgcctat caattttagc aactatgggt cggcatcagt aacaaattca
    58681 gttagcttac caacaataaa gcgtgctagt acagcgacaa tagcaagcgg aaatattggt
    58741 agtgcagttg ctattactat caaaagacaa tcagatactt ttagacacac attgaaattt
    58801 gattttaaag gtctaacagg cacgattgca tctttagttg acacatctta ctcatggacg
    58861 ttgcctgcat cactgtctgt cttgatacca aatgaccgaa gtggtacagg aactttgatt
    58921 gtagaaacat ttacaagtga tagtcaaaaa atcggtgaga acaagtatac ctttactgca
    58981 acagtgccag acattgctgc atataagcca atattggcaa gtattagttt atctgatgct
    59041 aacactctga caggtagttt gataactggc aataactatg tcaggaacat gagtaaatta
    59101 aaggtgtctt ttggtaactc ggcaggtgct aatggttcaa ctattagctc atataatgct
    59161 gaaattgttg aaaaaggcaa agctattttt ggaaatggta gtgtttttga tatgttggat
    59221 tttgttggaa cagcaacaat cagagctacg gtaactgata gcagaggtct aacatcagaa
    59281 ccagttgaca caaaaattaa tgttattgat tattttttac caattgttac aagtgcaaaa
    59341 gtagtcaggt ctcagcaaaa tcctgatatt ttacaagtct tgccatttgt taagattgca
    59401 ccaattatag ttggtggaat acaaaaaaac caactcaaaa tgtcggtatc tgttgcacca
    59461 tacaatactg gtatctatgc agttgatagt ggcgcagcta caaatacctg gtcaacaatt
    59521 tcccaaatgt caggtgcccc tttaaatctt ggcggcactt atgacaagtc aaaatcatgg
    59581 cttgttaaga tttcggtgag tgataaccta atgtcagcaa caccaataat ccaaccagtt
    59641 gcaagtgagt ttgtacttgt aactaaagca ccttctggtg ttgcatttgg gaaaatttgg
    59701 gaacatggta ttattgatgc caaaggcgat gtttatgttg atggtaatat ttattgtggc
    59761 gataagccaa tccaacaaaa acaactcgct cttaacaacg gcggttcttt tagacatgac
    59821 gacactgacc taaatagctt gcaagacaca ggtttttatt gtgtatttag aggtgctaat
    59881 agaccgaccg gagcaggccc tggctatgta actgttgtaa gacacgagac atcaaactat
    59941 gcttatcaac aattttacga ccgtacaaat aaaactatat ttaccagagt attggaaaat
    60001 ggggtttgga gcggttggag tgagtatgtt aaaaaagata gtttgcctac aacgatagac
    60061 acaggttggc agtcgattgg aaatggtttt agttataggc agacaggcag tacagtcacc
    60121 gttaagtaca actttgcgac gaatggcata gataagttga cggttggctc tatgcctacg
    60181 aatttgataa ctagcgacat gatgtttgca gtaacggctt ggactataca actcaatgtt
    60241 ttaaatgttc aagtgagcgc agacggtcgt attctgtggt ttaatccgtc aaaatggact
    60301 gttaacgtaa aaggtcaaat tcagtggacg atttaaagga ggaaaactat tggaaatttt
    60361 aaacaaatat cctgtaatgc tagaagataa aagcattgca aaagttaatg caattgtggc
    60421 agttgattta cctcacgtaa gaggtaactt aacttttgac ttaccagttg actttgataa
    60481 taaatctttt gcagaaacgc ttgaaaagtg tgagcagata ttttacgacg aaaagtataa
    60541 agataaagct cagtctgaaa aaatgactga actaagtaca tcaacatcaa caggcacaca
    60601 aacacttatc aatctgataa gtacgcttta cgcaaaagag gttttaaaag atgaagatct
    60661 tattgctatt ggttagaatt tttttacagg aagaagggat agatatgatg attaaattat
    60721 ttgcgattga cttatattat ggacgtatgg cttggtcaag ttttgttaaa aagggatttt
    60781 cagagtttat taataacaaa acaaaagagc aacttgcaat tatgtgcgat gaagaattac
    60841 ttgctgaaat tttagcaagt tagtgaggta gtcggatgac agtagaacaa gcagaaagaa
    60901 tcgctcaatc acaatttgtg tgggctattc tctttatctt gctttttatg attgtggttg
    60961 gttatctggt gcgaacgtct gataagcgtg agaaaaagct aatggatttc catgaccaat
    61021 caaaatcaga atctaacaaa cgtgaagagt ggctcaaagg tcacttagat aaaaatacag
    61081 aacagttaca ggacatttct cagaccattg gtgttgtcca aaaggagatg tcttatatga
    61141 gtgaccgcat tggtcgtcta gaaaaagagg agaaataaga tgaacgaatt tacaacacaa
    61201 atcattacag catcagtacc aatttttggt atcatcgcag gtattttaac gcatgaagtt
    61261 aaaaaactac ttatcaaaaa aggaggcgaa aaggctgtta aaatcgcaga aattgtcgct
    61321 cgtaatgctg ttgaggcagt tgaacaaata tctgtggaag ttggtattaa gggacaagat
    61381 aagctaacag aagctaaaac cgctgttatt gatggtttat cgcaatataa tattaatctt
    61441 acacagacac aacttgaaac ttttatcgaa gctgcggtta aacgcatgaa tgatgagtgg
    61501 aagaaaggtc aataatatgg taattaatat tgagcaagct atcgcatgga tggcatctag
    61561 aaaaggtaaa gttacttact cgatggacta tcgaaatggt ccttcatctt acgattgctc
    61621 tagctctgtc tattttgctc tacgctcagc aggcgcatca gataatggct gggcagtaaa
    61681 tacagaatat gagcacgatt ggttgataaa aaacggttat gttcttattg cagaaaatac
    61741 taattggaat gcgcaaagag gtgatatttt tatttggggt aaacgtgggg cttcggctgg
    61801 agcttttgga catactggta tgtttgttga tccagacaat attattcact gtaattatgg
    61861 ttataactca attacagtga ataaccatga tgaaatctgg ggatataatg gtcaaccgta
    61921 tgtttacgct tatcgttact ctgggaaaca gtcaaatgct aaggttgata ataaatcagt
    61981 tgtatctaaa tttgaaaaag agttagacgt taacacaccg ctaagtaatt caaatatgcc
    62041 atattacgaa gcaacaattt cagaagatta ttacgttgag tccaagcctg acgtcaatag
    62101 cactgataag gagttgcttg ttgctggtac tagagttcga gtttacgaaa aagtaaaagg
    62161 ctgggcaagg ataggcgctc cacagtctaa tcagtgggta gaagatgctt acttaattga
    62221 tgcaacagat atgtaaacca acagagcgat ataaatgtcg gtctgttaat ggtgtaagtt
    62281 acaccgcaac taaaaaaaca atttaggagg taaaattcct ttagataaga caaatgccct
    62341 cgcttttgcg ggggctattt ttattgaaat attgaaatct ctttataaaa atagtaaaat
    62401 agtttcgcta ttataaagaa agttgttatc aatgaataat ctagttcttc ctcagaattt
    62461 aaacaaatat aacattacga aaatcgttac caatttcaat agattacttg ctttaagtga
    62521 taacaggaca cttacagtag atatgagaaa cattgagttt gcggaaccta gtggagtaat
    62581 ttcgttatat aatatgttaa cttttgctac aaaaagaaaa gatgcaaaca tcaagtggtt
    62641 aatatgcgaa gaaagctctt taaataaacg tcaaaggcaa gctatgttgt atctagtaga
    62701 ttgtggcttt tttaaagtgt ttgataaatt ggtttataaa gagccggaac tgcgcccgac
    62761 tacttttgaa attaaattta ttaacactga acaaatagct caatggaagg taacagactt
    62821 taagaattgg ttacaaaagc aaactggcag aacaaatgag tttagttcta tttgtgtagc
    62881 ggttgacgaa atttttaata atattgcaga tcattctaag gaatctaagg gatgtatttt
    62941 tgggcaatac tatcccaaga acaaggagat tgtaatagca gtatctgatt tcggaatagg
    63001 aatccctcag tctataaagc gaaaatttaa aaaggatgag cctgacaaca agttaataga
    63061 attcgctctt caagagggtg tttctgcaga aactatacct caaaatagag gggcagggct
    63121 ttctaatatt gtaaatactt taactactaa caaagtcggg aactttacaa ttatatctaa
    63181 ttgtggtata gtctcggtat cagataataa aattactcaa agttattcgt ctgaggaatc
    63241 atatcctggt actttttttg aaattcgaat agatgtatcg aatgacaatt tatatgattt
    63301 agaagaggag gaagaattcg aatggtaacg ttaactgtca aagaactagc aaagaatttt
    63361 tctaacgata a
//
LOCUS       DATRTN010000004        57525 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.004, whole genome shotgun sequence.
ACCESSION   DATRTN010000004 DATRTN010000000
VERSION     DATRTN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 57525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57525
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.004"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(168..554)
                     /gene="rplQ"
                     /locus_tag="VBE22_000236"
     CDS             complement(168..554)
                     /gene="rplQ"
                     /locus_tag="VBE22_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEO7201715.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEASDKYTSTTALQKLFDDIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(569..1507)
                     /locus_tag="VBE22_000237"
     CDS             complement(569..1507)
                     /locus_tag="VBE22_000237"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEO7201716.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELDVEGP
                     AEITAGDILTDSDIEIVNPDHYLFTIAEGHSLKATMTVAKNRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKIKLADLGLGLKNDK"
     gene            complement(1557..1940)
                     /gene="rpsK"
                     /locus_tag="VBE22_000238"
     CDS             complement(1557..1940)
                     /gene="rpsK"
                     /locus_tag="VBE22_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEO7201717.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(1958..2323)
                     /gene="rpsM"
                     /locus_tag="VBE22_000239"
     CDS             complement(1958..2323)
                     /gene="rpsM"
                     /locus_tag="VBE22_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEO7201718.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLSTSKKILAAAGISEDIR
                     VKDLTPDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2341..2457)
                     /gene="rpmJ"
                     /locus_tag="VBE22_000240"
     CDS             complement(2341..2457)
                     /gene="rpmJ"
                     /locus_tag="VBE22_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEO7201719.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2483..2701)
                     /gene="infA"
                     /locus_tag="VBE22_000241"
     CDS             complement(2483..2701)
                     /gene="infA"
                     /locus_tag="VBE22_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEO7201720.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(2817..3455)
                     /locus_tag="VBE22_000242"
     CDS             complement(2817..3455)
                     /locus_tag="VBE22_000242"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEO7201721.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLIERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPILEHYRKLGLVTDIEGNQEITEVFADVEKALLELK"
     gene            complement(3550..4854)
                     /gene="secY"
                     /locus_tag="VBE22_000243"
     CDS             complement(3550..4854)
                     /gene="secY"
                     /locus_tag="VBE22_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEO7201722.1"
                     /translation="MFLKLLRDALKVKMVRNKILFTIFILLVFRIGTHITVPGINVKS
                     LEQMGELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLFLAFVQSIGITAGFNTLSSVALVKTPNVQTYLLIGAILTTG
                     SMVVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAITTIYEDFFVNVRSSAITNSYIF
                     VGILIVAVLAIVFFTTFIQQAEYKIPIQYTKLVQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFFQNGKEIPWLTKLQELLNYQTPVGMIIYAILIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSIRPGRETEEYMSSLLKKLATIGSVFLAFISLLPIIAQQALHL
                     SSSIALGGTSLLILIATGIEGMKQLEGYLLKRRYVGFMNTTE"
     gene            complement(4875..5315)
                     /gene="rplO"
                     /locus_tag="VBE22_000244"
     CDS             complement(4875..5315)
                     /gene="rplO"
                     /locus_tag="VBE22_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEO7201723.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRMPKRGFSNINAKEYALVNLDQLNVFEDGTEVTPVVLKEAGIV
                     RAEKSGVKILGNGELTKKLSVKAAKFSKSAEAAITAKGGSIEVI"
     gene            complement(5440..5619)
                     /gene="rpmD"
                     /locus_tag="VBE22_000245"
     CDS             complement(5440..5619)
                     /gene="rpmD"
                     /locus_tag="VBE22_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEO7201724.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVNAISHLVTVEEA"
     gene            complement(5634..6128)
                     /gene="rpsE"
                     /locus_tag="VBE22_000246"
     CDS             complement(5634..6128)
                     /gene="rpsE"
                     /locus_tag="VBE22_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEO7201725.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMVEVPMVGTTIPHEVRSEFGGAKVLLKPAVEGA
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6147..6503)
                     /gene="rplR"
                     /locus_tag="VBE22_000247"
     CDS             complement(6147..6503)
                     /gene="rplR"
                     /locus_tag="VBE22_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEO7201726.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSNGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADSARENGLKF"
     gene            complement(6604..7140)
                     /gene="rplF"
                     /locus_tag="VBE22_000248"
     CDS             complement(6604..7140)
                     /gene="rplF"
                     /locus_tag="VBE22_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEO7201727.1"
                     /translation="MSRIGNKVITLPAGVEIINKDNVVTVKGPKGELTREFNKNIGIT
                     VEGTEVTVTRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKALEMRGVGYRAQLQGSK
                     LVLSVGKSHQDEVEAPEGVTFEVPTPTTINVIGINKESVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(7250..7648)
                     /gene="rpsH"
                     /locus_tag="VBE22_000249"
     CDS             complement(7250..7648)
                     /gene="rpsH"
                     /locus_tag="VBE22_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEO7201728.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIADILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGQNGERVITNLKRISKPGLRVYTKHEDMPKVLNGLG
                     IAIVSTSEGLLTDKEARQKNVGGEVLAYIW"
     gene            complement(7803..7988)
                     /locus_tag="VBE22_000250"
     CDS             complement(7803..7988)
                     /locus_tag="VBE22_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEO7201729.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFQLCRVCF
                     RDLAYKGQVPGVTKASW"
     gene            complement(8006..8548)
                     /gene="rplE"
                     /locus_tag="VBE22_000251"
     CDS             complement(8006..8548)
                     /gene="rplE"
                     /locus_tag="VBE22_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEO7201730.1"
                     /translation="MANRLKEKYTNEVVPALTEKFNYSSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(8572..8877)
                     /gene="rplX"
                     /locus_tag="VBE22_000252"
     CDS             complement(8572..8877)
                     /gene="rplX"
                     /locus_tag="VBE22_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEO7201731.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVVVEGVALIKKH
                     QKPNNENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYNKKSGEVLD"
     gene            complement(8957..9325)
                     /gene="rplN"
                     /locus_tag="VBE22_000253"
     CDS             complement(8957..9325)
                     /gene="rplN"
                     /locus_tag="VBE22_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEO7201732.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9350..9610)
                     /gene="rpsQ"
                     /locus_tag="VBE22_000254"
     CDS             complement(9350..9610)
                     /gene="rpsQ"
                     /locus_tag="VBE22_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEO7201733.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(9636..9842)
                     /locus_tag="VBE22_000255"
     CDS             complement(9636..9842)
                     /locus_tag="VBE22_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEO7201734.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(9852..10265)
                     /gene="rplP"
                     /locus_tag="VBE22_000256"
     CDS             complement(9852..10265)
                     /gene="rplP"
                     /locus_tag="VBE22_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEO7201735.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEVAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10269..10922)
                     /gene="rpsC"
                     /locus_tag="VBE22_000257"
     CDS             complement(10269..10922)
                     /gene="rpsC"
                     /locus_tag="VBE22_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEO7201736.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(10935..11279)
                     /gene="rplV"
                     /locus_tag="VBE22_000258"
     CDS             complement(10935..11279)
                     /gene="rplV"
                     /locus_tag="VBE22_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEO7201737.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKNVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11295..11573)
                     /gene="rpsS"
                     /locus_tag="VBE22_000259"
     CDS             complement(11295..11573)
                     /gene="rpsS"
                     /locus_tag="VBE22_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEO7201738.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(11672..12505)
                     /gene="rplB"
                     /locus_tag="VBE22_000260"
     CDS             complement(11672..12505)
                     /gene="rplB"
                     /locus_tag="VBE22_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEO7201739.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTNTPEKSLLVSLKNKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YILAPKGLEVGQRIISGPEADIKVGNALPLANIPVGTVIHNIELQPGKGAELIRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRNRWKGVRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            complement(12523..12819)
                     /locus_tag="VBE22_000261"
     CDS             complement(12523..12819)
                     /locus_tag="VBE22_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEO7201740.1"
                     /translation="MNLYDVIKKPVITEKSMVALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVTVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(12819..13442)
                     /gene="rplD"
                     /locus_tag="VBE22_000262"
     CDS             complement(12819..13442)
                     /gene="rplD"
                     /locus_tag="VBE22_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEO7201741.1"
                     /translation="MANVKLFDQTGKEVSSVELNEAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVENLSFAAPKTAEFASVLSALSIDSKVLVILEEGN
                     EFAALSARNLPNVTVATATTASVLDIVNADKLLVTKEAISTIEGVLA"
     gene            complement(13466..14092)
                     /gene="rplC"
                     /locus_tag="VBE22_000263"
     CDS             complement(13466..14092)
                     /gene="rplC"
                     /locus_tag="VBE22_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEO7201742.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETN
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVVLIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14229..14537)
                     /gene="rpsJ"
                     /locus_tag="VBE22_000264"
     CDS             complement(14229..14537)
                     /gene="rpsJ"
                     /locus_tag="VBE22_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEO7201743.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(14743..16023)
                     /locus_tag="VBE22_000265"
     CDS             complement(14743..16023)
                     /locus_tag="VBE22_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO7201744.1"
                     /translation="MRYSKEIIQLAIPAMIENILQMLMGVVDNYLVAQLGVVAVSGVS
                     VANNIITVYQAIFIALGASIASLLAKSLAGSEKDDAISVCSQAIFLTLLIGAVLGIIS
                     IVFGQTFFKLLGTTKSVAQVGGLYLAIVGGGVVTLGMLTTLGSFLRVQGQPRLPMYVS
                     IFVNFLNAVLSGFAIFEWRYGLVGVAVSTLIARLIGICILAKYLPIKKIIKRMTWKIS
                     AQIWNLALPSAGERLMMRAGDVVIVAIVVQLGTNVVAGNAIGETLTQFNYMPGLGIAT
                     ATIILTAKYVGQKNRESIEETIQSSYYIGLVLMILISSFMLLAGKPLTQLFTNNPSAI
                     KGSLIVILLSFVGVPATIGTLVYTAAWQGLGNAKLPFYTTTIGMWLIRVVLGYLLGIV
                     FELGLLGVWMATIADNIFRWLFLKVHYHRYIQKM"
     gene            complement(16128..17618)
                     /locus_tag="VBE22_000266"
     CDS             complement(16128..17618)
                     /locus_tag="VBE22_000266"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEO7201745.1"
                     /translation="MAIVYQSTRDASNKVTASQAILKGLADDGGLFTPITFPKVDLDF
                     TKLKDASYQEVAKLVLSAFFDDFTEQELDYCISQAYDTKFDTTEIAPIVKIGDRYHLE
                     LFHGPTIAFKDMALSILPYLLTTAAKKQGVDDKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKNGVSYIQELQMITQAGQNTHVVAIEGNFDDAQTSVKEMFNNSLLRSKLSQ
                     QHMQLSSANSMNMGRLVPQIVYYIYAYAQLVKSKEISIGQPINFSVPTGNFGNILAAY
                     YASQIGLPVTKLICASNDNNVLTDFFKTQTYDKNREFKVTSSPSMDILVSSNLERLIF
                     HLLGDDAETTKKLMEDLVTTGEYALEARQANILESFVAGFATEQFVELDIKRLFDQYQ
                     YIEDPHTAVASAVYQAYQTETKDQTPTVIVSTASPYKFPSVVTKAITNKEEIQDFAAI
                     SILNDLSGVSLPKAVTDLQKAEVIHRTVVPTSNMRETVEECLGLKQ"
     gene            complement(17739..18755)
                     /gene="adhP"
                     /locus_tag="VBE22_000267"
     CDS             complement(17739..18755)
                     /gene="adhP"
                     /locus_tag="VBE22_000267"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEO7201746.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPKVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLRVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAVVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIAVYGAGGLGNLAVQYAKKVFNAHVVAVDINADKLQLAKEVGADLTVNGKEIK
                     DVAAYIQEKTGGCHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIRVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPQVFDEMERGLIQGRKVL
                     DFTK"
     gene            complement(18935..21577)
                     /gene="adhE"
                     /locus_tag="VBE22_000268"
                     /gene_synonym="adhC"
     CDS             complement(18935..21577)
                     /gene="adhE"
                     /locus_tag="VBE22_000268"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEO7201747.1"
                     /translation="MTEKTKAVETTDVALAIDTLVQNGLKALDEMRQLNQEQVDYIVA
                     KASVAALDAHGELALHAVEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEEDDV
                     TGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWIEQPSIDATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYKEFVEEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKSAVWIAEQAGFTVPEGTN
                     ILAAECTEVSEKEPLTREKLSPVIAVLKAESTEDGVEKARQMVEFNGLGHSAAIHTKD
                     ADLAREFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTDLMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVMTVPGFIAADTGMDVLTHATEAYVSQMANDY
                     TDGLALQAIKIVFDYLERSVKDADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPAATP
                     EEAVESYAKAVYDLGTRLGIKMNFRDQGIDEKEWKEKSRELAFLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYEERPGRRK"
     gene            complement(21846..23624)
                     /locus_tag="VBE22_000269"
     CDS             complement(21846..23624)
                     /locus_tag="VBE22_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO7201748.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFKNSFPGGFVGVDIFFTFSGFL
                     ITALLIDEFSKTKKIDFVSFCRRRFYRIFPPLVLMVLVTIPFVFLVKSDFRASIGSQI
                     MTALGFTSNFYEILTGGNYESQFIPHLFVHTWSLSIEVHFYVLWGLTVWLLSKRSKDQ
                     KQLRGTLFLISMGVFGVSFLTMFVRAFFVDNFSTIYFSTLSHIFPFFLGAMVATISGI
                     REITGRFKKNIKNLTLKHNLIMMGSAFAGLMILTFALDFDNRLTYLFGFVLSSIFASV
                     MIYNARILHEHTPDISEPFVITYLADISYGMYLFHWPFYIIFSRLSPNWIAVILTVVL
                     SAVFSTLSFYIIEPFILGRKPKFLDYEFDLLPYKKWLFSIGGVLTLITVVTMLTAPSI
                     GSFETELLQNSLQQARTNTNRTHTLAAGDAGALSDVTVIGDSVALRSSAAFNKLLPEV
                     QLDAAVSRNFSKSFDIFENRIQNKALSKIVVLAVGVNSLDNYKTDLSQFIKSLPKGHR
                     LIIVTPYNAKNMSQVTTVRDYELSLMKKYNYITVADWYKVATEHPEIWGNTDGVHYSD
                     SDTTGADLYVSNVKKAIQKSAQRAAK"
     gene            complement(23621..24001)
                     /locus_tag="VBE22_000270"
     CDS             complement(23621..24001)
                     /locus_tag="VBE22_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201749.1"
                     /translation="MKNFKITRTHLEILSLIIIVVFGLSVFTLTTSSQGVLSYDGGKI
                     KYVGSIVNHHMTGKGKLTYENGDYYKGDFVNGVFEGKGTFVSVHGWSYTGDFKKGQPD
                     GQGRLNAKNKKVYKGTFKQGIYQK"
     gene            complement(24008..24445)
                     /locus_tag="VBE22_000271"
     CDS             complement(24008..24445)
                     /locus_tag="VBE22_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEO7201750.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMANLDKEDSLSVESRATSTWEHG
                     NPIHRGTQAILQRYQIPYINDKGSQPISKDDLAYFDYIIGMDEQNVKDLKALSQGTYD
                     DKISLFCDGGVPDPWYTGDFEETYRLVNDGCKQWLTLINNSRD"
     gene            complement(24597..25595)
                     /gene="ruvB"
                     /locus_tag="VBE22_000272"
     CDS             complement(24597..25595)
                     /gene="ruvB"
                     /locus_tag="VBE22_000272"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001902090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEO7201751.1"
                     /translation="MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEA
                     AKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAIEKSGDLVAILNDLE
                     PGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPR
                     IANRLLKRVRDYAQIMGDGLIDDNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGG
                     PVGLGTLSVNIAEERDTVEDMYEPYLIQKGFIMRTRTGRVATDKAYEHLGYQRFDK"
     gene            complement(25884..26786)
                     /locus_tag="VBE22_000273"
     CDS             complement(25884..26786)
                     /locus_tag="VBE22_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7201752.1"
                     /translation="MLKHFGSKVRNLRVTRNITREDFCGDETELSVRQLARIESGQSI
                     PNLTKAHYIAKQLNVKLDILTGGESLELPKRYKELKYKLLRTPTYGDANRLAVREAYF
                     DEIYEVFYEELPEDERLIIDCMQSKLDVHFSVNDNFGITILHDYFDQIKKKKEYTTND
                     FVMIDLYLLCFSINYGMKSLYSLENYHFIMSKLLEQDNLLPEDNFQLNNVLLNHVELA
                     FQFKQKKYVQQIIHRSNAIMTEIHDFQKRPILSLIEWKYLLIIEKDRTKAETCFKQSI
                     LFAELIGDLYLKGKLIEEWNKDLT"
     gene            complement(26939..28237)
                     /gene="purB"
                     /locus_tag="VBE22_000274"
     CDS             complement(26939..28237)
                     /gene="purB"
                     /locus_tag="VBE22_000274"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEO7201753.1"
                     /translation="MLERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VTKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVAEKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIHPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEELDELFNPIYYTKRVDDIFERLGLEK"
     gene            complement(28323..29426)
                     /locus_tag="VBE22_000275"
     CDS             complement(28323..29426)
                     /locus_tag="VBE22_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201754.1"
                     /translation="MASGEKAKIQRKKSGAWSMTYKTLDGNVVATFRPMFKKTGSHCY
                     ISTSKMTKSDGNNNFSIQIYYKGTSNVKIKMFDDNPEHTMQFSKKKFANNMLNRDEST
                     TNHSYDKVLEGDLTPFMGQFSSDSFNQTIVDSGMTYGGYEPKDYYEGRTTVFPAITKT
                     GYWNGITSHGSYVIKNDDLPKKIKNYYEIHVYGANSGAINSTGSLKRIFYLVPPKVEG
                     PDGKVLSKRQVFEVGANNQLISLSYQSPDWWKRYKKSGLEKDLDVDAIYRGDFSSLKG
                     TWRNGKGEVLTINDDGSTDDGERISIGSTEKSRLPSVGMSSGNFGVSVYLFQIGVSNP
                     YIENGHTSDTTRPRLAIGLNPGDFPTDAYYYRY"
     gene            complement(29792..30049)
                     /locus_tag="VBE22_000276"
     CDS             complement(29792..30049)
                     /locus_tag="VBE22_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEO7201755.1"
                     /translation="MIKIIVHAFINSGKIGVVEVLFATSDDKLLMTKMSEFQRHYPGD
                     YLAIYDLPLDTDLNKLSHYPSVAISKEDFHEEKANETNENS"
     gene            complement(30059..31132)
                     /gene="purK"
                     /locus_tag="VBE22_000277"
     CDS             complement(30059..31132)
                     /gene="purK"
                     /locus_tag="VBE22_000277"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEO7201756.1"
                     /translation="MNLFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCSASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSAGQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLEGFDLTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAECVLEEFVDFDLEISVIVSGNGQDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKELQLSGTLCVEMFVTADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGQPLPPIKLHAPAVMLNVLGQHVQQAIDYVAENPSAHLHIYGKLE
                     AKHNRKMGHVTVFTEDADEVEEF"
     gene            complement(31119..31610)
                     /gene="purE"
                     /locus_tag="VBE22_000278"
     CDS             complement(31119..31610)
                     /gene="purE"
                     /locus_tag="VBE22_000278"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEO7201757.1"
                     /translation="MKTPIISIIMGSKSDWGTMQKTAEVLDNFGIAYEKKVVSAHRAP
                     DLMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKTRALSGLDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSN
                     ELI"
     gene            complement(31891..33153)
                     /gene="purD"
                     /locus_tag="VBE22_000279"
     CDS             complement(31891..33153)
                     /gene="purD"
                     /locus_tag="VBE22_000279"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEO7201758.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKDVDQVFVAPGNDGMTLDGLDL
                     VNIGISEHSRLIDFAKENEIAWTFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVETIIKPVLEGMIAEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFAENSKALLSNGGRVYMLVTTEGSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIK"
     gene            33436..34239
                     /locus_tag="VBE22_000280"
     CDS             33436..34239
                     /locus_tag="VBE22_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO7201759.1"
                     /translation="MTIYDQIESALDLMTDLEREIACYFMGQPISKDALASTIVTKQL
                     HISQAALTRFAKKCGFKGYREFVFEYLKSHETISQQLYGLQNDNTKKVFMNYQEMVSK
                     SADIIDEEQLLEVSHMIEQADRVYFYGKGSSSLVAKEFKIRLMRLGVICEALDDTDSF
                     SWTNSIVNDRCLVIAFSLSGNTNSVIGALKIASSHGAKTVLFTKQPHTIDYAFDKIIQ
                     VASARHLDYGNRISPQIPMLIMVDIIYAQFLDINKIEKERIFRETIIQR"
     gene            complement(34262..35239)
                     /locus_tag="VBE22_000281"
     CDS             complement(34262..35239)
                     /locus_tag="VBE22_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO7201760.1"
                     /translation="MIETMSLDDMREYLGQDQIPEDFDDFWKKQTMKYQGNIEYRLDK
                     KDFNITFAQAYDLHFKGSNDSIVYAKCLFPKTNKPYPVVFYFHGYQNQSPDWSDQLNY
                     VAAGYGVVSMDVRGQAGQSQDKGHFDGITVKGQIVRGMISGPNHLFYKDIYLDVFQLI
                     DIIATLESVDSNQLYSYGWSQGGALALIAAALNPKIVKTVAVYPFLSDFRRVLDLGGV
                     SEPYDELFRYFKYSDPFHKTENSVLKTLAYIDVKNFAHRISCPVVLLTALKDDICPPS
                     TQFAIFNRLTSTKKHLLLPDYGHDPMTVQVKDYIFDQLTGSQFTKQKIE"
     gene            complement(35247..36128)
                     /locus_tag="VBE22_000282"
     CDS             complement(35247..36128)
                     /locus_tag="VBE22_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO7201761.1"
                     /translation="MTRTVAIDIGGTMIKHGIVDNLGCIVEASELATEAYKGGPGILQ
                     KVCQIIDNYLTEGSIDGIAISSAGMVDPDEGCIFYSGPQIPNYAGTQFKKVLEDTYQV
                     RTEIENDVNCAGLAEAVSGSAKDSSIALCLTIGTGIGGCLIIDKTVFHGFSNSACEVG
                     YMHLSDGDFQDLASTTALIADVAKAHGDEISRWDGRRIFQEAKEGNEKCIASIDRMIN
                     YLGQGIANMVYVVNPEKVVLGGGIMAQKDYLQDKLSESLKRNLVTSLAEKTAIVFAQH
                     ENQAGMLGAYYHFKNRG"
     gene            complement(36145..37062)
                     /locus_tag="VBE22_000283"
     CDS             complement(36145..37062)
                     /locus_tag="VBE22_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEO7201762.1"
                     /translation="MKDLQKYQGIIPAFYACYDDKGDICPERVKALTNYFIDKGVQGL
                     YVNGSSGECIYQSVADRKLVLENVMSVAKGKLTVIAHVACNNTKDSVELAMHAEAIGV
                     DAIAAIPPIYFRLPEYAIADYWNTISQAAPQTDFIIYNIPQLAGVALTSDLYRKMLQN
                     PQVIGVKNSSMPVQDIQNFVAIGGENHIVFNGPDEQFLGGRLMGAAAGIGGTYGVMPE
                     LYLTLNQLIVDKDLEKARELQFTINDIITKLCSGHGNMYAVIKSVLEINEQLTIGSVR
                     LPLASVTEEDKPIIKEAAEMIRHAKKQFC"
     gene            complement(37059..37721)
                     /locus_tag="VBE22_000284"
     CDS             complement(37059..37721)
                     /locus_tag="VBE22_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEO7201763.1"
                     /translation="MKKANQLIAAIFDVNNTFMQGCNVVFDLALLNLLFMITCLPLVT
                     IGAAKISLYRTLWQKLEGDQTNLLILYIKHLKKEWFQGMLLGLVELSILVVIIFDLTI
                     LHYQIGFIVSFLKITCYAFLLLTVMTSIYLFPMAARYEMSLLDTVKKSFIMACLNLKW
                     TGVLMFLLIMTWFIMVQSSLLFMLTVSAIFIFAYTAFAYFKIIILQKQFAYFSKQQGD
                     YQ"
     gene            complement(37741..38184)
                     /locus_tag="VBE22_000285"
     CDS             complement(37741..38184)
                     /locus_tag="VBE22_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEO7201764.1"
                     /translation="MIYDHLLNLTHYKDINPNLDLAIDYLLSHDLRNLDIGTYHISPE
                     VILMVQSNQLSESFDHIFEYHKKYLDIHYVIEGHEVIKLGKGDKVEVEEYLGDIGFIK
                     CSEETSFDLRDNYIAFFFPEEAHQPNGMGSLGNYVKKGVLKVLMA"
     gene            complement(38197..39027)
                     /locus_tag="VBE22_000286"
     CDS             complement(38197..39027)
                     /locus_tag="VBE22_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO7201765.1"
                     /translation="MKKKTFSAYNFLTALILCLLTVLFIFPFYWIMTGAFKSQPDTII
                     IPPQWWPKAPTLENFKALTVQNPALRWLWNSVFISIMTMFLVCCTSSMAGYVLAKKRF
                     YGQKILFSLFIAAMALPKQVVLVPLVRIINFMGIHDTLWAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFINVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(39037..39924)
                     /locus_tag="VBE22_000287"
     CDS             complement(39037..39924)
                     /locus_tag="VBE22_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7201766.1"
                     /translation="MRTNKLKMRETMIAYAFLAPILLFFLIFVFAPMVMGFVTSFFNY
                     SMTQFTFIGLANYNRMFHDSIFMKSLINTVIIVIGSVPVVVFFSLFVAANTYEKNVFS
                     RSFYRCVFFLPVVTGSVAVTVVWKWIYDPMSGILNYILKSGHVIEQNISWLGDKHWAL
                     LAIIIILLTTSVGQPIILYIAAMGNIDNSLCEAARVDGANEMQVFWQIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIALISFAQFKILGNDVEY"
     gene            complement(40012..41328)
                     /locus_tag="VBE22_000288"
     CDS             complement(40012..41328)
                     /locus_tag="VBE22_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7201767.1"
                     /translation="MSIKKSVIGFCLGAAALSMFACVDSSQSVMAAEKDKVEITWWAF
                     PTFTQEKAKDGVGTYEKKVIKAFEKKNPNIKVKLETIDFTSGPEKITTAIEAGTAPDV
                     LFDAPGRIIQYGKNGKLADLNDLFTDQFIKDVNNKNIIQASKSGDKAYMYPISSAPFY
                     MAFNKKMLKDAGVLKLVKEGWTTSDFEKVLKALKNKGYTPGSFFANGQGGDQGPRAFF
                     ANLYSAPITDKEVTKYTTDTKNSVKSMKKIVEWIKKGYLMNGSQYDGSADIQNFANGQ
                     TAFTILWAPAQPKTQAKLLESSKVDYLEVPFPSEDGKPDLEYLVNGFAVFNNKDENKV
                     KASKKFITFIADDKKWGPKDVIRTGAFPVRTSFGDLYKGDKRMMKISKWTQYYSPYYN
                     TIDGFSEMRTLWFPMVQSVSNGDEKPADALKDFTQKANDTIKKAAK"
     gene            complement(41375..42073)
                     /locus_tag="VBE22_000289"
     CDS             complement(41375..42073)
                     /locus_tag="VBE22_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEO7201768.1"
                     /translation="MPHLSKEAFKKQIKNGIIVSCQALPGEPLYTESGGVMPLLALAA
                     QEAGAVGIRANSVRDIKEIQEVTNLPIIGIIKREYPPQEPFITATMTEVDQLASLDIA
                     VIALDCTLRERHDGLSVAEFIQKIKGKYPEQLLMADISTFEEGKNAFEAGVDFVGTTL
                     SGYTDYSRQEEGPDIELLNKLCQAGIDVIAEGKIHTPKQANEINHIGVAGIVVGGAIT
                     RPKEIAERFISGLS"
     gene            complement(42320..43624)
                     /locus_tag="VBE22_000290"
     CDS             complement(42320..43624)
                     /locus_tag="VBE22_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO7201769.1"
                     /translation="MKMNKKVLLTSTMAASLLSVASVQAQETDTTWTARTVSEVKADL
                     VKQDNKSSYTVKYGDTLSVISEAMSIDMNVLAKINNIADINLIYPETTLTVTYDQKSH
                     TATSMKIETPATNAAGQTTATVDLKTNQVSVADQKVSLNTISEGMTPEAATTIVSPMK
                     TYSSAPALKSKEVLAQEQAVSQAAANEQVSTAPVKSITSEVPAAKEEVKPTQTSVSQS
                     TTVSPASVAAETPAPVAKVAPVRTVAAPRVASVKVVTPKVETGASPEHVSAPAVPVTT
                     TSTATDSKLQATEVKSVPVAQKAPTATPVAQPASTTNAVAAHPENAGLQPHVAAYKEK
                     VASTYGVNEFSTYRAGDPGDHGKGLAVDFIVGKNQALGNEVAQYSTQNMAANNISYVI
                     WQQKFYSNTNSIYGPANTWNAMPDRGGVTANHYDHVHVSFNK"
     gene            complement(43771..44670)
                     /locus_tag="VBE22_000291"
     CDS             complement(43771..44670)
                     /locus_tag="VBE22_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO7201770.1"
                     /translation="MNKWLVKASSLVVLGGMVLSAGSRVLADTYVRPIDNGRITTGFN
                     GYPGHCGVDYAVPTGTIIRAVADGTVKFAGAGANFSWMTDLAGNCVMIQHADGMHSGY
                     AHMSRVVARTGEKVKQGDIIGYVGATGMATGPHLHFEFLPANPNFQNGFHGRINPTSL
                     IANVATFSGKTQASAPSIKPLQSAPVQNQSSKLKVYRVDELQKVNGVWLVKNNTLTPT
                     GFDWNDNGIPASEIDEVDANGNLTADQVLQKGGYFIFNPKTLKTVEKPIQGTAGLTWA
                     KTRFANGSSVWLRVDNSQELLYK"
     gene            complement(44863..46413)
                     /gene="purH"
                     /locus_tag="VBE22_000292"
     CDS             complement(44863..46413)
                     /gene="purH"
                     /locus_tag="VBE22_000292"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEO7201771.1"
                     /translation="MTRKRALISVSDKSGIIDFAKELKNLGWDIISTGGTKVALDDAG
                     VETITIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVN
                     LYPFKETILRPDVTYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELAD
                     AGQTTFETRQRLAAKVFRHTASYDALIAEYFTAQVGEAKPEKLTITYDLKQAMRYGEN
                     PQQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKH
                     MNPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPS
                     YSEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQ
                     VVTDRQPTEQEATALEFSWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQ
                     AKDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQESIDAANKHGLTMIF
                     TGVRHFRH"
     gene            complement(46432..47052)
                     /locus_tag="VBE22_000293"
     CDS             complement(46432..47052)
                     /locus_tag="VBE22_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201772.1"
                     /translation="MTNNNSFNGGHNIGSGNSAGRDQIINITNSQAEQPVAKYDVEPI
                     WRSPITMAVLSWIGFFTSIGSLLPLYNFLKPIISWAKNSFDGNIPNVQGSQIYLFIFL
                     GLFIFSVVIFSLRRITKSQTRYPLGFNYAISGVGKRLNIEKLHITPCPKCGGKLKYYN
                     KPYDWINHYDENGNIKKIKITKRVPALECKRNSEHFWYVDSAGTKI"
     gene            complement(47071..47634)
                     /gene="purN"
                     /locus_tag="VBE22_000294"
     CDS             complement(47071..47634)
                     /gene="purN"
                     /locus_tag="VBE22_000294"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEO7201773.1"
                     /translation="MKIAVFASGNGSNFQVIAEQFQVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKTAYEQAIVDLLDKHEIDLVCLAGYMKIVGETLLSAYEGRIINIHP
                     AYLPEFPGTHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGVERKEWNTK"
     gene            complement(47802..48824)
                     /locus_tag="VBE22_000295"
     CDS             complement(47802..48824)
                     /locus_tag="VBE22_000295"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEO7201774.1"
                     /translation="MSEKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSQTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQASAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVKEGDILLGLASSGIHSNGYSLARRVFADYTGDEVLPELEGKQLK
                     DVLLEPTRIYVKAALPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLDVNPENVDRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            complement(48852..50306)
                     /locus_tag="VBE22_000296"
     CDS             complement(48852..50306)
                     /locus_tag="VBE22_000296"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEO7201775.1"
                     /translation="MTYEVKSLNEECGVFGIWGYPQAAQVTYFGLHSLQHRGQEGAGI
                     ISNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISSRKELEKQGAIFNASSDTEILMHLIRRSHNPSFMGKVKE
                     ALSTVKGGFAYLLMTEDKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGATEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAVDEVCDIIGADSLTYLSIDGLIKSIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            complement(50718..54443)
                     /locus_tag="VBE22_000297"
     CDS             complement(50718..54443)
                     /locus_tag="VBE22_000297"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEO7201776.1"
                     /translation="MNKRIFVEKKADFGIKSASLVKELTHNLQLASLKDLRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDRLLTEAEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLLGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEVQAFS
                     VSDKTISNLDFFETYQADDFAAYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQATYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVVLLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVGPSDVDAFIA
                     ACNKENIDAVVVATVTEKPNLVMTWNGETIVDLERCFLDTNGVRVVVDAKVVDKDLTV
                     PEARTTSAETLEADMLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQYGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFGQPVSALLGSIEAQIQFGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFEAIQAQHKIT
                     AASAVKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSVEEIADVVKIG
                     QTQADFTVTVNGNDLAGASLLSAFEGKLEEVYPTEFEQVDAIEEVPAVVSDVVIKAKE
                     IIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEAAIAESVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLAGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQKLFESAVKYFTGK"
     gene            complement(54566..55270)
                     /locus_tag="VBE22_000298"
     CDS             complement(54566..55270)
                     /locus_tag="VBE22_000298"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEO7201777.1"
                     /translation="MTNQLIYTGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNMAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGHVLETPIVEFYYKNDNLNDPFINDEHVKFLGIVNDEEIAYLKGETRHINEL
                     LKDWFAQIGLNLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRDLG
                     SLTDVYQVVLEKLIAL"
     gene            complement(55394..55633)
                     /locus_tag="VBE22_000299"
     CDS             complement(55394..55633)
                     /locus_tag="VBE22_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085641.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO7201778.1"
                     /translation="MSRDEVFEKMLELLRQQLGDPQLDITPESSLHDDLAIDSIALTE
                     FIINLEDVFHLEIPDEAVEHMSSVQQLLDYIIEHK"
     gene            complement(55644..56636)
                     /gene="plsX"
                     /locus_tag="VBE22_000300"
     CDS             complement(55644..56636)
                     /gene="plsX"
                     /locus_tag="VBE22_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEO7201779.1"
                     /translation="MKKIAIDAMGGDYAPKAIVEGVNQAISDFSDIEVQLYGDQKKIE
                     KYLTVTERVSIIHTEEKINSDDEPAKAVRRKKQSSMVLGAKAVKDGVAQAFISAGNTG
                     ALLAAGLFVVGRIKGVDRPGLMSTMPTLDGVGFDMLDLGANAENTASHLHQYAILGSF
                     YAKNVRGIEVPRVGLLNNGTEETKGDSLHKEAYELLAAEPSINFIGNIEARDLMSSVA
                     DVVVTDGFTGNAVLKTMEGTAMSIMGSLKSSIKSGGVKAKLGALLLKDSLYQLKDSMD
                     YSSAGGAVLFGLKAPIVKCHGSSDSKAVYSTLKQVRTMLETQVVDQLVDAFTDE"
     gene            complement(56714..>57525)
                     /locus_tag="VBE22_000301"
     CDS             complement(56714..>57525)
                     /locus_tag="VBE22_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7201780.1"
                     /translation="NWFVPVIGAVLIQVIGIIVSRRLVVKLLPDDYKFNLYIALLLFV
                     PILASILLFLIYFQKRDFTSLGFTKDNNVLTTISGFITGSLLMFFIYICHWLSGFVEI
                     SSSNSFSFGPFFFLFLAYFIQSLTEEILFRGYVMTTVTKFKGSFAGVLCNSMLFSFIH
                     FRNYGITAIALFNLFLLGIIFSILFNMTKNILFVTGVHTTWNFTMGCVLGNKVSGGDS
                     PVSLFRITENSSFALWNGGDFGFEGGVLCSLILVGCLAYFSLESVRVSVRY"
BASE COUNT        17369 a        12203 c         9913 g        18040 t
ORIGIN      
        1 ctgcttacga aatataaaca aaattgtttg catttcgtaa acaagcgtta cctatttaac
       61 aatatatgat gagtgttccc gctgagaata attctcagcg gtagaccaga gctagactaa
      121 gaatcgattg attccatcat cataacactc aacaaaattg ataaaaatta tactaattca
      181 ataattgcca ttggggcagc atcgccacgg cgtggttctg ttttaagaat acgagtgtat
      241 cctccgttac gttcagcata acgaggtgcg atgtcgtcaa aaagtttttg aagagctgtt
      301 gttgatgtat atttatcaga agcttcatca tagttttctg atgcaatttc attacgtaca
      361 taagcagcag cttgacgacg agcatgtaaa tcaccacgtt tacctagagt aatcattttt
      421 tcaactgttt tacggatttc tttagcacgt gcttcagtag ttacaattga ttcgttgata
      481 agaagatcgg ttgtcaaatc acgaagcatt gccttacgtt gtgagctagt gcgtcctagt
      541 ttacggtaag ccattatgtc ctcctatttt atttatcgtt ttttaatcca agacctagat
      601 cggcaagttt aattttaact tcttcaagac tcttacgtcc taagtttcgg actttcatca
      661 tttcaggctc agttttttct gttaaatcaa atactgtatt aattccagca cgttttaaac
      721 agttatatga gcgcactgac aaatcaagtt cctcaattgt gcggtcaagc actttctcat
      781 cgttcacttt ctctgtttcc ttcattactt cagttgcttt agcaacctct gttaaatcag
      841 taaacaagtt taagtgttca attaagacgc gagctgaaag accaagagca tcctcaggaa
      901 tgattgtgcc atttgtcatg atttcaatag ttaatttatc aaagccatcg ttactaccta
      961 cacgagcagg ttcaacttga taattaactt ttttcactgg tgtatagatt gaatctacag
     1021 ccaatgttcc cactggtgca tcatcttttt tatttccttc tgctggaaca taacctcggt
     1081 ttttagcaac tgtcattgtt gctttcaaac tgtgaccttc agcaattgtg aaaagataat
     1141 ggtcagggtt tacaatttcg atatcactat ctgttaaaat atctccggca gtaatttctg
     1201 caggaccttc aacgtctagt tcgataatct tttcgtcttc gacgtaagat ttcacagcaa
     1261 gtccttttac attaaggatg atttgcatca catcttcacg tacacctggg atagtatcaa
     1321 attcgtgtaa tactccatca attttgattg atgtgactgc tgcacctgga agtgaagaca
     1381 gaagcacacg acgaagagag tttcctagag ttgtaccgta gccacgttct agtggttcga
     1441 tgacaaatct gccgtaatct ttattttcat caatttttgt tattattggt ttttcaaact
     1501 caatcattta gttactccct cttaaacgaa aagttgtgta ctataaaatg ctatgattat
     1561 acacgacgac gttttggagg acgagcacca ttatgtggta caggagtcac atcacgaatt
     1621 gcagttactt caagaccagc tgcagcaagg gcacggatag cagattcacg acctgaacca
     1681 ggacctttta cagtaacttc aacagtttta agaccgtgtt cttgtgcaga tttagcagca
     1741 gcttcagcag ccatttgagc agcaaatgga gtagatttac gagaaccttt aaatccaaga
     1801 gcaccagctg atgaccatgc aagagcgtta ccatgcacat ctgtaatcat aacaatagtg
     1861 ttattgaatg tagcgtgaat atgagcaaca ccagattcga tgttcttttt cacacgacgt
     1921 ttgcgtgttg gtttagccaa ttgttttacc tcctatttta ttttttctta cctgcaatcg
     1981 caacagcttt acctttacga gtgcgagcgt tatttttagt attttgtcca cggacaggaa
     2041 gtccacgacg gtgacggatt ccacggtatg aaccgatttc catcaaacgt ttaatattca
     2101 agtttacctc acgacgtagg tcaccttcaa ctttgattgc atcaacttca cgacggatag
     2161 cgtcctcttg atctggtgtc aaatctttaa cgcggatatc ttcagaaata cctgcagctg
     2221 ctaagatttt tttagatgta gaaagaccaa ttccgtatac ataagtaagt gaaattacta
     2281 cacgtttgtc atttggaata tcaactccag caatacgagc cattttatct cctttctata
     2341 ttatccttga cgttgtttgt gttttggatt tgtcggacaa attaccataa cacgaccgtt
     2401 acgacgaata actttacagt attcgcaaat tggtttaacc gatggtctta ccttcatgtt
     2461 ttaatccctc caattatttc gactatttaa agcggtatgt gatgcgtcca cgtgttaaat
     2521 cgtatgggct catttcaaca gtaacacgat cacctaccaa aatacggatg tagtttttac
     2581 ggatttttcc tgatacagtt gccaaaattt ggtgtccatt ttccaactca acagtaaaca
     2641 ttgcgttagg catagtttca acaactttac cttcaatttc aatcacatct tcttttgcca
     2701 cgcaaaagta ccccctaaat ttcgatttga tgtcctctat aaagaggtaa caattataag
     2761 tcagactaac ttagtatatc attactaatc tacttatgca agtagattag cataatttat
     2821 ttgagttcta gcaacgcttt ttcaacatct gcaaaaactt ctgttatttc ttgattacct
     2881 tcaatatctg taacaagacc aagcttacga tagtgttcaa gaataggttc tccttgagca
     2941 atattaacgt ccaagcgacg tttgacagtt tcaggcttat catcttcacg ttgatagtaa
     3001 tcttcttctt tataatctac tggtgggttg aacactttgt ggaaagtttc accagtttta
     3061 cgattgataa tacgaccact caaacgctct ataagacatg atggatccac tttaatatta
     3121 ataacaccat ctaagcgtag tcctagttct tcaagcgtag catctaaggc gtgtgcttgt
     3181 tcaatagtac gtggatatcc atcaagtaaa aaaccttttt ctgcgatatc atcctcagct
     3241 aagcgctctt ttacaatccc gtttgttact tcatcaggaa ccaattcacc tttatcaata
     3301 taacttttag ctaaacgtcc catttcggtt tgattagcca ttgcggcgcg gaacatatcc
     3361 cctgttgaga tgtgagcaac accaaattct tcaacgatct tagctgcttg agtaccttta
     3421 ccagcaccag gcaaacccat aattaaaaga ttcattatag tctccttatt ttgtttttaa
     3481 ataaatccaa cacagcatgt cggacttatt aaaaaacaaa atagaaggtt gacagagtca
     3541 acctatattc tattctgttg tattcatgaa tccaacatag cgtctcttca aaagatagcc
     3601 ctcgagttgt ttcatacctt caatacccgt tgcaatcaaa atgagtaagc ttgtcccacc
     3661 taacgcaata cttgatgaca aatgcaacgc ttgttgagca ataattggta acaaggaaat
     3721 aaatgctaga aatactgaac caattgttgc taattttttc aatagcgatg acatatattc
     3781 ttctgtttct cgtcctggtc gaatacttgg aatgtaggac gaattttttt gaagattctc
     3841 tgctgtcttt tcagggttta cttgtacaaa agtatagaag aaagagaaca gaataatcaa
     3901 gatagcatag ataatcatac cgactggggt ttgataatta agtaattctt ggagtttagt
     3961 aagccaagga atttctttgc cattttgaaa gaaaggaata atcgtacttg gaattgttgt
     4021 aatcgagcta gcaaagataa cgggaataac accagctgga tttactttta gaggaagata
     4081 tgaacttgta ggagctcctt gaacaagttt tgtatattgg attggaattt tgtattcagc
     4141 ttgttgaata aatgttgtaa aaaatacaat tgctaatact gctacaataa gaatcccgac
     4201 aaagatgtaa gaatttgtaa ttgctgaaga gcgtacattt acaaagaaat cttcgtagat
     4261 agttgtaatt gcacttggaa tggaagaaat gatacccgca aagataatca tagacactcc
     4321 atttccgaat cccttatcag taatttgttc acctagccaa gtaaccacca tactacctgt
     4381 tgtaaggatt gcaccaataa gcaaatatgt ttgtacattc ggagttttaa ctaaagcaac
     4441 acttgataga gtgttaaaac cagcagtaat accaattgat tgtacgaatg caaggaacaa
     4501 agaaatataa cgtgtagctt ggttaagctt acgacgacct acctctcctt gttttcccca
     4561 ttcaacaaac ttcggaagaa tatccatttg aagtagctga acaacaatag acgcagtaat
     4621 gtatgggcta actcccatag agaataccga aaagtttctc attgcattac cactaactaa
     4681 gttaagcata tttagaaatg gaagttcacc catttgttct aaactcttaa cattgatgcc
     4741 cggtactgtg atatgcgttc cgatacgaaa tacaagaagg ataaagatag taaataaaat
     4801 tttatttctt accatcttta ctttgagtgc atctctaagt aattttaaaa acataatgag
     4861 ttacctctca ttaattagat gacttcgatt gaaccacctt tagcagtgat agctgcttca
     4921 gcagatttag aaaacttagc tgctttaaca gacaatttct tagttaattc accattacca
     4981 agaattttaa cgcctgattt ttcagcacgc acgattccag cttctttaag aacaactgga
     5041 gttacttcag taccatcttc aaagacattt aattggtcaa ggttcacaag agcatattct
     5101 tttgcattga tgtttgagaa cccacgtttt ggcatacggc ggaacaatgg agtttgtcca
     5161 ccttcaaaac caaggcgtac tccaccacca ctacgcgctt tttgtccttt ttgaccacgt
     5221 ccagaagttt taccattacc tgaagatgta ccgcgaccca cacgattgcg gactttacgt
     5281 gatccttctg caggttttaa ttcatgaagt ttcattattt tttctcctta tttgtaaaat
     5341 gctaacgcct ttacgagggg aaaggtactc cccgcaaaga ctcgcctata cgatttgttt
     5401 aaatcgcacc aataacaatc agtgcgattt agcatttaat taagcttctt caacagttac
     5461 taagtgagag atagcgttaa ccataccacg gatggcagca ttatcttcct taacaactga
     5521 agaatttaat ttaccaagtc caagggcaac aactgtttta cgttgttctg gtttgcgacc
     5581 gattggagac ttagtcaaag taattttaat ttgagccatt ataatcccct ttcttatgct
     5641 aagtcagaaa cagaaatgcc acgtaaagca gcaacttctt ctgcacgttt taattgtttc
     5701 aagccctcaa cagttgcgcg aacaatgttg attggagtgt ttgaaccaag tgatttagat
     5761 gtaatatctg caacacctgc taattcgatg acggcacgaa cagcaccacc agcggcaact
     5821 ccagcaccct caacagctgg tttcaacaat actttcgctc caccaaattc tgaacgaact
     5881 tcgtgaggaa ttgttgtacc gaccatagga acttcaacca tgtttttctt agcagcttca
     5941 acagctttgc gaatagcttc tggtacttct tgagctttac ctgtaccgaa accaacacga
     6001 ccattacggt caccaacaac tacaagagct gcaaaacgta gacgacgtcc acctttaaca
     6061 acttttgtaa cgcggttaat cgcaacaacg cgctcttcaa gttctactgc attatcttta
     6121 aatgccatta ttaagtgtcc tccctattag aatttcaatc cgttttcacg agctgagtca
     6181 gccaaagctt ttacacgtcc gtgatagaga tatccaccgc ggtcaaacac cacttcagaa
     6241 atacctttag ctactgcgcg ttcagcaaca agtttaccga caacaacggc ttgttctgtt
     6301 tttgttccgt tagaaacttc tttatcaaga gttgatgcac ttgcgagcgt tacacccgct
     6361 acgtcatcaa ttacttgagc gtagatgcct gtattagaac ggaaaatgtt caaacgtggg
     6421 cgatcagcag ttccagagag tttaccgcga acgcgacggt ggcgtttttg gcggatttta
     6481 tttttatctg gtttcgaaat cacaattttc acctcttaaa tatattgtta tgtcaaaatg
     6541 acaatattaa aatgaaagga tagagaattt gcagaaaaga ttctacaaaa actatcaatc
     6601 ctattattta cctgttttac cttctttacg gcgtacgaat tcaccaacgt aacgaatacc
     6661 tttaccttta tatggttctg gtgaacgtaa gctacgaacg taagcagcag tttgtccaac
     6721 gctttctttg ttgataccga ttacgttgat tgttgttgga gttggcactt caaaagtaac
     6781 gccttctgga gcttcaactt cgtcttggtg agacttacca acagaaagaa caagttttga
     6841 tccttgaagt tgtgcacggt aaccaacacc gcgcatttca agagcttttt tgaaaccttc
     6901 agaaacacca acaaccatgt tgttcaagtt cgcacgagtt gtaccatgaa ttgttttcat
     6961 ttcttttgag tcgttaggac gagtaactgt tacttcagtc ccttcaacag tgattccaat
     7021 atttttatta aactcacgag tgagttcgcc tttaggtcct tttacagtaa caacattatc
     7081 cttgttaatg atttcaacac ctgcaggcaa tgtaattact ttattaccaa tacgtgacat
     7141 gtttattttc tcctgttaaa ttgtcaagct gcttgagcaa ctagttttca cggggactca
     7201 tgagaccaca ggattatact gtgactcaat gagcatttta ggtaaaattt taccaaatat
     7261 atgcaagtac ttctccacca acattttttt ggcgagcttc tttgtcagtc aaaagacctt
     7321 cagaagttga tacaattgca ataccaagtc cgttaagaac tttaggcata tcttcatgtt
     7381 tagtataaac acgaagacct ggttttgaaa tacgtttcaa gttagtgata acacgttcac
     7441 cattttgtcc atatttaagg aacacacgga tgatgccttg tttgtcatct tcaataactt
     7501 caacgttttt tacaaaacct tcacgtttaa ggatatcagc aatccctttt ttaatgtttg
     7561 atgcaggtac ttctaacact tcatgttttg cttggttagc attacgaata cgagttaaaa
     7621 agtctgcaat tgggtcagtc ataaccattt tttaattctc ctcttattag cagtttgaat
     7681 ttaatcactt gctaattaat gagtccttac ggacgttata agactaggtc ttagtatatt
     7741 tgcgtttaaa aatcagtgcc attatcaatg acacctttta aacacaaagc atcattaatt
     7801 tattaccaag atgctttagt tacgcctggt acttgacctt tataagccaa gtcacggaag
     7861 caaacacggc aaagttggaa tttgcggtaa actgagtgtg gacgtccgca tttttcacaa
     7921 cgagtgtaag cttgcgtaga gaacttcgct gggcgtttgt tcttagcaat catagatttt
     7981 ttagccaatt tatttacctc ctagattact ttgcaaaagg cattccaagg ccttttagca
     8041 attcacgtga ctcttcatca gtgttagcag tagtaacgat aacgatatca agaccacgta
     8101 ctttatcaac gtcatcaaag ttgatttctg ggaagatcaa ttgttcttta actccaagag
     8161 tgtagtttcc acgtccatca aatgatttcg ttggaacacc atggaagtca cgtacacgtg
     8221 gaagtgaaac agaaactaat ttatccaaga attcgtacat acgttcacca cggagggtaa
     8281 cttttgcacc gatcgctaca ccttcacgaa gacggaagcc agcgattgat ttcttagctt
     8341 tagtaataag tggtttttga cctgaaataa gtgctaactc agcagcagct ttctcaagat
     8401 ttttagcgtt tgatacagca tcaccaacac ccatgttaag aacgattttc tcaacttttg
     8461 gcacagccat aacagatgaa tagttaaatt tttctgtcaa cgcaggaaca acttcattag
     8521 tatatttttc ttttaagcga tttgccatta tgcttctcct ttccttcgtg attaatcaag
     8581 cacttcgcct gattttttgt tgtaacgaac ttttttgccg tcaacaactt tgtaaccaac
     8641 acgtcctgct acaccatttt tatcaagaac ttgaacgttt gacacatgga ttggagcttc
     8701 tttttcaacg atagcgcctt gagggttttc gttatttggt ttttgatgtt tcttgatgag
     8761 ggctacgcct tcaacaacaa ctttgtttac ttttggaaga gctttaagaa ctacagcttc
     8821 agtgcctttg tccttaccag caataacgcg aactttgtcg ccttttttta caaacatttg
     8881 agtttttctc ctatttttct tacgcctcga aaggcaccct aggaaatcct aggggactac
     8941 gtttgttaat ctgagattaa agtacttctg gtgccaatga aacgatcttc atgtagccac
     9001 cttcacgcaa ttcacgtgca actgggccaa agatacgagt tccgcgagga gttttatcgt
     9061 cacggatgat tacagcagca ttgtcgtcaa atttgatgta tgaaccgtct gggcggcgtg
     9121 caccagtttt tgtacgaacg ataacagctt taaccacatc acctttttta actgctccac
     9181 caggagtagc ttgttttaca gaagcaacga ttacgtcacc gatgttagcg aatttacgtc
     9241 ctgaaccacc aagtactttg atagtcaaga tctcacgagc accgctatta tcagcaactt
     9301 tcaagcgagt ttcttgttga atcatttcaa ttttctcctt ttagtttgat tagataataa
     9361 cagctttttc cactacttcc acaagacgga aacgttttgt agctgaaagt gggcgagttt
     9421 ccatgatacg aacgatatcg ccttctttag caacgttgtt ttcatcatgt gctttatatt
     9481 ttttagaata gttgatacgt ttaccataga ctgggtggtt acgtttagtt tcaactacaa
     9541 ctgtgattgt tttgtccatc ttgtcagaca ctacgcgtcc gtaaagagtt ttacgttgat
     9601 tacgttccat ttagaatttc tcctttccca atctattatt tcatttctga ttgcacagtt
     9661 ttaacacgtg caatttgttt cttaacttcg tcaaggcgag cagtcttttc aagttgacct
     9721 gctgcagctt ggaaacgaag atcaaacaat tcttttttga gttcgttttc tttcttagca
     9781 agttcttctt gagacaatcc acgaagctct ttaacaaaat ctttaatttc ttgaagtttc
     9841 atgtcttctc cttattctgc ttcacgtttt acgaatttgc atttaactgg taatttgtgg
     9901 ctagcaagac gtaatgcttc acgtgcaact tcttcagaaa caccagcaat ttcaaacatc
     9961 actttaccac gtttaactgg tgctacccaa ccttcaggtg cccctttacc agaacccata
    10021 cgtacaccga tagcttttgc agtgtatgat ttgtgtggga agattttaat ccaaacttta
    10081 ccaccacgtt tcatgtaacg agtcatcgca atacgggcag cttcgatttg acggtttgta
    10141 atccagtggc tagttgtagc ttgaagtccg tattcaccga atgaaacttc tttaccacct
    10201 ttagcctcac cgcgcatttt tccacggaat tcgcgacggt gtttaacacg tttaggtact
    10261 aacatttgtt atttgcctcc tttagtgttt ttacgagctg gaagaacttc tccacggtaa
    10321 atccaaactt taacgccaag tttaccataa gttgtgtcag cttcttccca agcatagtcg
    10381 atatcagcac gaagcgtgtg aagtggaaca gttccttctg aataaccttc agcacgagcg
    10441 atatcagcac cgttcaaacg accagaaact tgagttttaa tacctttagc tcctgcacgc
    10501 attgtacgtt ggattgcttg tttttgagca cgacggaaag caacacgttg ctctaattga
    10561 cgagcaatat tttcaccaac aaggtgagca tctaaatcag gttgtttgat ttcaatgatg
    10621 ttgatgtgta cttgttttcc agttaatttg ttaagttgac cacgaagagc gtcaacattt
    10681 gctccacctt taccgataac catacctggt ttagcagtgt gaagtgaaac aataactttg
    10741 tttactgcac gttcaatttc aatagttgaa actgatgcgt cagctaattc tttattaatg
    10801 aatttacgga ttgcaagatc ttcatgaagg taatccgcgt attctttttc agcataccat
    10861 ttcgcatccc agtcacggat gattccgaca cgcataccaa ttggatgtac tttttgaccc
    10921 acgattttac ctccttattt ttctgataca actacagtta cgtgagttgt acgtttgttg
    10981 attggtgaag ctgaaccttt cgcacgtgga cggaaacgtt tcattgttgg tccttcgtta
    11041 gcgaatgttt cagatactac caagtttgct ttttccaaac caaagttgtt ctctgcattt
    11101 gcaattgctg agttaagagt tttctcaata acacgagctg ctttgtttgg agtgaatttt
    11161 aagattgcga ttgcgtcagc aacattctta ccacggataa gatcaagtac taaacgtgtt
    11221 ttacgaggtg aaacacgcac tgtacgagcc attgctttag ctgaagtaat ttctgccatt
    11281 gtgtcctcct cctattaacg acgtgttttc ttgtcgtcag ctgcgtgacc tttgtaagta
    11341 cgtgttggtg caaattcacc aagtttgtga cctaccatgt cctcttggat ataaacagga
    11401 acatgtttac gtccatcata aactgcgatt gtgtatccga tgaaacttgg gaaaatcgtt
    11461 gaacgacgtg accaagtttt gattactttt ttcttttcgt catttgcttg agcttcaact
    11521 tttttcatta aatgctcgtc gacgaaaggt ccttttttaa gactacgtcc cattttgtag
    11581 ttttctcctt taaataatgt accacaacgg cctgtacact atagtgtact accgagctgg
    11641 cggatttgta taagattatg ctattaacct attatttttg gttacgacga cgaacaataa
    11701 gtttgtctga tttagctttc ttattacgag ttttaagtcc aagtgcaggt ttaccccatg
    11761 gagtagatgg tgctttacga ccaactggtg ctttaccttc accaccaccg tgtgggtgat
    11821 cattagggtt cattacagaa ccacgaactg tagggcgaac acctttccaa cgattacgtc
    11881 ctgctttacc aatgttaaca agtgattgtt gttcattacc aacagtacca attgttgcac
    11941 ggcaagtacc aaggatcata cgtacttcac ctgattgaag gcgaacaaga acatatttac
    12001 cttcttgacc aagtacttga gcagaagcac ccgcggcacg aatcaattca gcacctttac
    12061 ctggttgcaa ttcaatatta tggataactg taccgactgg aatgttagca agtggaagtg
    12121 cgttaccaac tttgatatct gcttctggac cagaaataat gcgttgacct acttcaaggc
    12181 ctttaggcgc aagaatgtaa gctttaacac catcagtata gtgtacaaga gcaatatttg
    12241 cagtacgatt tggatcgtat tcaattgttt taacaactgc ttcaacacca tctttattac
    12301 gtttgaagtc aatcaaacgg taatgacgtt tgtgtccacc accttggtga cgaacagtaa
    12361 tacgtccgtt gttgttacgt cctgctttat tctttagtga aacaagcaat gatttctcag
    12421 gagtgtttgt agtgatttct gcaaaatcca aagaagtcat attacgacgg ccatttgtcg
    12481 ttggtttata aactttaata cccacgttat ttcctcctta gattattcag cttcagcagc
    12541 aaacaactcg attgcttttg aatcagctgt aagagtgatg atagcttttt tagtttttga
    12601 agtgaaacct gtgtaacgac caacacgttt tgcttttggt ttaactgtca cagtgtttac
    12661 acttgcaact tttactccgt caaatgcagc ttcaacagct tgtttgatca aaagtttgtg
    12721 tgcacgtgtg tcaacttcaa atgtatattt ccctgcttca agagcaacca ttgatttctc
    12781 agtgataaca ggttttttga ttacgtcata caaattcatt atgcaagaac cccctcgatt
    12841 gtagagattg cttctttagt aacaagaagt ttgtctgcgt taacgatatc aagaacactt
    12901 gcagtagttg cagtagcaac tgtaacgttt ggaaggttac gcgctgaaag tgcagcaaat
    12961 tcgttacctt cttcaaggat tacaagtact tttgaatcaa tgctaagtgc tgaaagaact
    13021 gacgcaaatt cagcagtttt tggagctgca aatgaaaggt tttctacagc tacaaattta
    13081 tcttcagcaa cttttgctga ataaactgat ttcaacgcaa ggcgacgaac tttttgtgga
    13141 agtttgtagc cgtatgaacg tggagttggt ccaaagacaa caccaccacc acgccattgt
    13201 ggtgagcgga tagaaccttg acgagcgcgt ccagttcctt tttgacgcca tggtttacgt
    13261 ccaccaccag atactgctga acggttttta accgcgtgag ttccttggcg aaggctagcg
    13321 cgttggctga ttacaacatc aaaaacaact gattcgtttg gttcaatacc gaagatagct
    13381 tcgttaagct caactgagct aacttcttta ccagtttggt caaatagttt aacgtttgcc
    13441 attttaactg ttttctcctt tcttattatt tagcagcttt aactgctgat ttgatagtga
    13501 taagagattt cttagcacct ggtacgttac ctttgatgag gacaacgttt ttctctggga
    13561 taacttgtac aatttcaagg ttttgaactg ttacacggtt accacccata cgtcctgcca
    13621 agcgtttgtt tttgaaaacg cggttaggcg caacaggtcc catagaacct ggacggcgat
    13681 ggtaacgaga accgtgagcc ataggaccac gtgattgacc atggcgtttg ataacacctt
    13741 ggaaaccttt accttttgat gtccctgtga cgtcaacaac atcaccagct tcaaattgtt
    13801 ctacagataa ttctgcacca acttctaagc cttcaatgtt tttgaattca cgaatgaagc
    13861 gcttaggagc tgtgtttgct tttgcaacat ggcctttggc aggtttgtta ctcaatactt
    13921 cacgtttgtc atcaaaacca acctgaactg cttcgtaacc gtttgtttca acagttttaa
    13981 cttgaagcac aacgtttgga gttgcttcaa tgacagtaac agggatgaat tcgcctgatt
    14041 cagtgaagat ttgagtcatt cccacttttt tccctaagat tccttttgtc atgagaaaat
    14101 atttcctttt cttattttat ttcaaaaagt tttttatgag cgttttttgt gctcaaaaaa
    14161 gttttttacg agcgtttttt gtgctcaaaa aagtttttta cgagcgtttt ttgtgctcag
    14221 aaatcaaatt aaagtttgat ttctacgttg acaccacttg gaagatcaag tttcatcaaa
    14281 gcatcaacag ttttttgtgt tgggttgatg atgtctacca aacgtttgtg tgtacgcatt
    14341 tcaaactgtt cgcgagaatc tttatatttg tgagtcgcac gaataattgt gtaaagactg
    14401 cgttcagttg gaagcggaac tggtccagca actgtagcac ctgtacgtgt tgcagtttca
    14461 acgatttttt ctgccgctgt atcaagtgta cggtgttcgt acgccttcaa acggatacgg
    14521 atttttttgt ttgccatctt ttctcctctc gtctatttac gataataggc tagctcctca
    14581 agaaaaccaa cacgagttgc gtggcaatgc aacctagcgt gtcgcaacct cttgcatcat
    14641 agctaaagct gttcatacac agcaccagta tatgatatca aaaaaaaaca gcacacgcaa
    14701 gcattatttt tttattttcg aaaaaaatgc aaccatttct ggttacattt tttgaatata
    14761 tcgatgatag tgaactttca aaaacagcca cctaaagata ttatctgcta tcgtagccat
    14821 ccaaacccca agcaaaccta attcaaaaac tattcctaat aaataaccaa gaactactct
    14881 gattaaccac atgcctatag tcgttgtgta aaatgggagt ttagcatttc ctaatccttg
    14941 ccaagctgct gtataaacca aggtcccaat agtagcaggt actcctacga aagatagcaa
    15001 aataacaatc aaacttcctt taatggcaga aggattattt gtaaataact gtgtcaatgg
    15061 tttgcctgca agaagcataa aacttgatat aaggatcatt agaactaagc caatataata
    15121 cgaactttga atagtttctt ctatagattc tctatttttt tgtcctacat attttgcagt
    15181 taggataatt gtagcagttg cgatgccaag ccctggcata taattaaatt gtgtcaaggt
    15241 ttctccaata gcatttcccg ctacaacatt tgtacctaac tgaacaacaa tagcaactat
    15301 aacaacatct ccagctcgca tcatcagacg ttctcctgct gatggtaagg caagattcca
    15361 aatttgtgca ctgattttcc aagtcattct ttttattatc tttttaatag gaagatactt
    15421 cgcaaggata caaataccaa ttaatcttgc gataagagtt gatactgcca ctccgaccaa
    15481 accatagcgc cactcaaaaa tagcaaaacc tgataagact gcattgagga aattgacaaa
    15541 aatactgaca tacattggta aacgaggttg cccttgcaca cgtaaaaatg agcctagggt
    15601 ggttaacatt cctaaagtaa caacacctcc acccacaatt gctaaatata aaccaccaac
    15661 ttgtgcgaca ctttttgttg tccctaaaag tttaaagaaa gtttgtccaa aaacaatcga
    15721 gataattcct aatactgccc ctatcagtaa tgttagaaaa atggcttgag aacatactga
    15781 aattgcatca tccttctcac tacctgctaa cgacttggcc aatagacttg ctatactcgc
    15841 ccctaaagct ataaaaatag cttgataaac agtaattata ttattagcaa ctgaaacacc
    15901 tgatactgct acaacaccta actgagccac tagataatta tcaactactc ccatgagcat
    15961 ttgtaagata ttttcaatca tagctggtat agctaactga ataatctctt tactataacg
    16021 cacagtatct cctttctatt acccaggatc attaaaaaga gagtgatggg aaaatttcta
    16081 aaatgtttct ttctcgctct aaatatttta aaacaatcgt atttttatta ttgtttcaaa
    16141 cctagacact cttcaaccgt ttctcgcata tttgaggtag gtactaccgt tctatggatt
    16201 acttcggcct tttgtaaatc tgtaaccgcc ttaggtaaag aaacacctga caaatcgttt
    16261 aagattgaaa tagcagcgaa atcctgtatc tcttctttat ttgtaatagc ctttgtaaca
    16321 acactcggga acttataagg acttgctgtt gacacaatta ctgttggagt ttgatcttta
    16381 gtttctgtct gataagcttg ataaacagct gatgctacag ctgtatgcgg atcctctata
    16441 tactgatact ggtcaaataa acgtttgatg tctaattcaa caaattgttc tgtcgcaaat
    16501 cccgctacaa agctctctaa aatatttgct tgcctagcct ctaaggcata ctccccagta
    16561 gtcactaaat cttccattaa ttttttagtc gtttctgcat catcgcctaa aaggtgaaag
    16621 attaatcgtt ctagattaga ggagaccaaa atatccatag agggacttga tgtcacttta
    16681 aattctcgat ttttatcgta ggtttgtgtc ttaaaaaaat ctgttagaac gttattatca
    16741 tttgaggcac aaatgagttt ggtaacaggt aatccaattt gactagcata atatgctgct
    16801 aaaatattac caaaatttcc tgtaggtaca ctaaaattaa ttggttgacc aattgagatt
    16861 tcctttgact taactaattg agcatatgca tatatatagt aaacaatttg aggaacaagg
    16921 cgacccatgt tcattgaatt ggctgatgat aactgcatat gctgctgtga taattttgat
    16981 cgaagaaggg aattattgaa catttcttta actgaagtct gtgcatcatc aaaatttcct
    17041 tcaatcgcaa caacatgggt attttgtccg gcttgtgtta tcatctgcaa ttcttggata
    17101 taactaacac catttttagg ataaaagaca ataatctcag ttcctggaac atctgcaaaa
    17161 cctgccatcg ctgccttacc agtgtcacct gatgttgctg ttaaaataac gattttatca
    17221 tcaacccctt gttttttggc tgctgttgtt agcaaataag gaagaatgga taaagccata
    17281 tctttaaatg caattgtagg accgtgaaat aattcaaggt gataccggtc tccaatcttt
    17341 actattgggg ctatttcagt tgtatcaaat ttagtatcat aagcttgact aatacagtaa
    17401 tccaattctt gttcagtaaa atcatcaaaa aaagctgaca aaacgagttt agccacttct
    17461 tggtaggaag catccttaag tttagtgaaa tctaaatcaa cttttggaaa agtgatgggg
    17521 gtaaagagtc ctccatcgtc agctaatccc tttagaatcg cttgactagc tgtcacttta
    17581 ttactagcat cacgtgttga ttgatatact attgccataa gaatccctct ttatataaat
    17641 atcttttagt tattgtaaca caatttaata acatgtccat tgttcgttat ataattaacc
    17701 cccctttatt aaaataaagg gggggcgtaa tatcattatc actttgtaaa gtcgagaact
    17761 ttacgtcctt gaatcaatcc tctttccatt tcatcgaata cttgaggagc tgtatccaca
    17821 ggcacttttt caaccactgg tactaccaaa ccttctgctc caaatgcgaa ggcttcttct
    17881 aaatctttac gagttccaac taaggaacca accacacgaa tcccatcaag tactgttttt
    17941 acaatactca attccatata ttctgatgga agaccaacag ctaccactgt acctccagca
    18001 cgaacgctgt caatcgcctg attaaacgca actttagaaa cagcagtcac aaccactcca
    18061 tgacaaccgc ctgtcttttc ttgaatatag gccgcaacat cttttatttc tttgccatta
    18121 actgtcaaat ctgctccaac ctctttagct aattgaagtt tatctgcgtt aatatcaaca
    18181 gctacaacat gagcattgaa tacctttttt gcatattgga ctgctaagtt tccaagacca
    18241 cctgcaccat acactgcaat ccactgacca ggagcagctc cagcttcttt gatagccttg
    18301 tatgttgtta ctccagcaca agtgattgat gatgcttgag ctggatctaa tccctcagga
    18361 accttaaccg catagtccgc ggtaacgaca gcgtattcac tcataccacc atcaacactg
    18421 tatccagcat ttttaacact acgacaaagc gtctcacgtc ctgtagtaca gtattcgcaa
    18481 tgaccgcacc cttcaaagaa ccatgcaata gagacacgat caccaaccct caaagacgtt
    18541 acgccttctc caatttcttc tactatacca atgccttcgt gacccaaaat gcgtcctggt
    18601 acctggccga aatctccatg tgcaacgtgt aaatcagtat ggcacacacc acagtattct
    18661 acttttacaa gtgcttcacc atgaccaact tttggaagat catgctcaac aacttcaaca
    18721 cctgtactag cttgattaac aacaaccgct ttcatgataa aacctcctag aaataaatat
    18781 atcttatttt gtaatcgttt acattttata ttgtattatt cttcacaaac tatgtcaagt
    18841 gattttcaga aaattttttg aaaaatattc ttaactggtt aatctttttg aaactaaaaa
    18901 tcccactcaa tagagtgaga ttcttagact gctgttactt acgacgtcct ggacgttcct
    18961 cataaccata ataagcatct tcgatgattt cttgcatgtg atctaccatc ggaaggcgtg
    19021 gatttgctgg tgaacattga tcttcatatg ctaagaaagc tagttcacga gatttttctt
    19081 tccattcttt ttcatcaatg ccttggtcgc ggaaattcat cttaatacct aaacgtgttc
    19141 caaggtcata cacagctttt gcatatgatt cgacagcttc ttctggtgtt gctgctggta
    19201 agccgagtaa tttagcgata tcttgatatt tttcatcagc acgatagtaa ttgtacttag
    19261 gccatgttgc tgttttagct ggacgagttc cattatagcg gataacatac ggcaaaagaa
    19321 ttgcatttgt acgtccgtgt acggtatgga attgtgcgcc aattttatgc gccattgagt
    19381 gagaaatacc taagaatgca ttggcgaaag ccatacctgc catagttgac gcattgtgca
    19441 tcttttcacg tgcttcaaaa tcagcatctt ttactgagcg ttctaagtag tcaaacacaa
    19501 ttttaattgc ttgaagggca agtccgtctg tataatcatt tgccatctgt gaaacataag
    19561 cctctgttgc atgtgtcaat acatccatac ccgtatcagc cgcaataaaa ccaggaacag
    19621 tcataacaag ggccggatca acgatagcta cagttggtgt taatgaataa tcagcgattg
    19681 gatatttacg attatttgct ttgtcagaga taacggcaaa tggtgtcact tcagaacctg
    19741 ttccagaagt tgttggaata gcaacaaatt ttgttttctt accaagttct gggaatttga
    19801 acgcacgttt acggatatcc atgaattttt gaacgaggtc atggaaatca acttctggtt
    19861 gttcatagaa gagccacata actttcgcag catccatagg agaaccacca cctaatgcaa
    19921 taatagtgtc tggtttgaag gtacgcatca aatctgttcc tttcattact gttgtaatgt
    19981 ctggatctgg ttctacttca gcaaaaatct gataaacaac tttattgcga cgaagatcaa
    20041 gttgttcaat gatacgatcc aagaaaccaa gctcaaccat ggcgtgatct gtaacaatca
    20101 tgacacgttc aacatcgcga catttttgca aatattgaat agaatcacgt tcgaagtatg
    20161 tttttgaagg gactttaaac cattgcatat tatttctacg tcttcctact tttttgatat
    20221 tcaagagatt tatagcacta acgttatcac caactgagtt acgtccatac gaaccacaac
    20281 caagagtcaa tgatggcaag aaagcattgt aaacatcccc aataccacca aaagtagaag
    20341 gagaattcca aataacacgg atagcacgaa ttctagttcc aaattcacga gctaagtctg
    20401 catcttttgt atgaatagca gctgagtgtc ctagaccatt aaactcaacc atttgacgag
    20461 ctttttcaac accatcttct gtagattcag ctttcaatac ggcaataaca ggtgatagtt
    20521 tttcacgtgt caaaggttct ttttcactaa cttcagtaca ttctgcggca agaatatttg
    20581 ttccttctgg aacagtaaat ccagcttgtt cagcaatcca aactgctgat tttccaacaa
    20641 tatttggatt taattttgca ccagcacagt ttttgctatt tgcttttgca ccaaaacaga
    20701 attcttcaag aagagctttt tctttcttat taacgaaata agtatgataa gatttaaatt
    20761 cttcaacaaa ttctttataa atttctttat caataataac agcctgttct gatgcacata
    20821 ccataccgtt atcaaatgat ttagacataa caatatcgtg agcggcttgt cggatattag
    20881 ctgatttttc aacataagct ggaacattcc cagcacctac accaagggca ggtttcccac
    20941 atgagtaggc agctttaacc atcgcattac cacctgtcgc aaggatagtg gcaataccat
    21001 catggttcat caaagcatta gttgcatcaa tcgatggttg ttcaatccat tggacacaat
    21061 tttcaggagc tccagcagca atagcagcat cacgaacaat acgtgctgca tgtgctgatg
    21121 attcttgtgc tgatgggtgg aaggcaaaaa taattggatt acgtgtttta agagagatta
    21181 atgacttgaa aatagcagtt gaagtcgggt tggttgttgg cgtaatacca cagacaactc
    21241 ccactggttc agcaattaat gtcaagcctg tgacatcgtc ttcttcaata actcccactg
    21301 tctttgtatg acgcatgttg ttaacgacgt gttcacatgc aaataaattt tttgtcgctt
    21361 tatcttcaaa gacaccacga ccagtttctt ctactgcatg aagagcaagt tctccatggg
    21421 catccaaggc tgcaacagaa gctttagcta caatataatc aacttgttct tgattaagtt
    21481 gacgcatctc atcaagtgct tttaaaccgt tttgaaccaa tgtatcaatt gcaagagcaa
    21541 catctgtagt ttctaccgct ttagtttttt cagtcatttg agactccttt tgatttgtta
    21601 aatgtttcac aaatttgttt acgagattat tatagcatat ttaaaaactt tgtaaatagt
    21661 ttcacaactt ttttgtgaac ttttttccaa tctttcggaa acatttcttg aaagcgcttt
    21721 aattgaactt gttttttcat tactaatttt cagattaaag ccaatcgatt tcatttgtat
    21781 caccataaac ggaggtatct attattcgtt ttcacaaaaa agccgagtac atgactcgga
    21841 cttgtttatt ttgcagctcg ttgagctgat ttttgaatag cttttttgac gttactaaca
    21901 tataaatcag cccctgtagt atcgctatca ctataatgca caccatctgt atttccccag
    21961 atttctggat gttctgtcgc aaccttatac cagtcagcaa ccgttatgta attatatttt
    22021 ttcattagcg agagttcata atcacgcact gtcgtaactt gactcatatt tttagcatta
    22081 taaggcgtta ctataatcaa acggtgtccc ttaggcaaag atttaataaa ttggctcaag
    22141 tctgtcttgt agttatctaa tgaattcaca ccaactgcca aaacaacaat tttggacaag
    22201 gctttatttt gaatacggtt ttcaaaaata tcaaatgatt tactaaaatt acgactcacc
    22261 gcagcatcta attgtacttc tggtaacaat ttattaaaag cagcacttga ccttaaggca
    22321 actgaatctc cgattaccgt cacatcacta agagcacctg catcccctgc ggctagtgta
    22381 tgtgttcggt tagtatttgt tctagcttgt tgtaacgaat tctgcaataa ttctgtctca
    22441 aaacttccta tacttggagc tgtcaacatt gtcactacag taataagagt tagtacccct
    22501 ccgatactaa ataaccactt tttgtaaggc aacaagtcaa attcataatc aaggaattta
    22561 ggttttctac ctaaaataaa aggttcaatg atataaaacg ataatgttga gaagactgct
    22621 gataatacta ctgtcaaaat aacagctatc cagtttgggg ataaacgact aaaaataata
    22681 taaaatggcc aatgaaaaag gtacataccg taactgatat cagcaagata agtaatcacg
    22741 aaaggttctg aaatatctgg tgtatgctcg tgtaaaatac gtgcattgta aatcattaca
    22801 ctagcaaaaa tgctagacaa aacaaaacca aaaagatatg ttagccgatt atcaaaatca
    22861 agtgcaaaag ttaaaatcat tagaccagca aaagcactac ccatcatgat taaattatgc
    22921 ttcaaagtca gatttttgat atttttcttg aatcgtccgg taatctctcg aataccagat
    22981 attgtagcca ccatcgctcc taaaaagaac ggaaatatat gtgataatgt agagaaatat
    23041 atcgttgaaa aattatcaac gaaaaaggct ctaacaaaca tggtaaggaa gctgactcca
    23101 aaaactccca tggaaataag gaacaaagtc cccctcaatt gcttttgatc ttttgatctt
    23161 ttcgataaga gccataccgt taagccccat aaaacataaa aatggacttc aatagaaaga
    23221 ctccaggtat gcacaaataa gtgagggata aattgacttt cgtagttacc accagtcaag
    23281 atttcgtaaa agttgctggt aaaacctaaa gcagtcataa tttgagagcc aatacttgct
    23341 ctaaaatcac ttttaaccag aaatacaaaa ggtattgtca ccaaaaccat caatactaat
    23401 ggtgggaaaa tacggtaaaa acgtctacga caaaaactaa caaaatcaat ttttttcgtt
    23461 ttagaaaatt catcaatcaa taaggccgta atcagaaaac cagaaaatgt aaagaagatg
    23521 tcaacgccaa caaatcctcc tggaaaacta ttcttaaaga aatggtaaag taaaacaagt
    23581 agtagtcctg ttatacgtac caaagaaaac cattttattc tcatttttga taaatcccct
    23641 gtttaaatgt ccccttatag actttcttat ttttagcatt gaggcgacct tgtccatcag
    23701 gttgaccttt tttaaagtca cctgtatagg accagccatg aactgaaaca aatgttcctt
    23761 tcccttcaaa gaccccattt acaaaatcgc ccttatagta atccccattc tcataggtta
    23821 atttaccttt accagtcata tggtgattaa caatagagcc cacatatttt atcttgccgc
    23881 catcatagct aaggactcct tgactagaag tcgttaaggt aaatactgat aaaccaaaaa
    23941 caacaataat tatcaaagat aatatttcta gatgagttcg tgttatttta aaattcttca
    24001 tttctactta atctctacta ttatttatta gcgttaacca ctgtttacag ccatcattga
    24061 ctagacgata ggtttcctca aaatcaccag tataccatgg atcaggaaca ccaccatcac
    24121 aaaaaaggct aatcttatca tcgtaggttc cttgtgatag agcttttaaa tccttgacat
    24181 tttgctcatc cataccgatg atataatcaa aatatgctaa atcgtcttta gaaataggtt
    24241 gtgacccttt atcgttaatg taaggaattt gataacgctg caaaatcgct tgcgttcccc
    24301 tatgtatagg atttccatgc tcccatgttg aagtcgcacg actctcaacc gagaggctat
    24361 cctccttatc taaattcgcc ataacaaact cagccatcgg gctacggcag atatttccaa
    24421 gacaaacaaa acagactttc ttcatagcaa actccttttg gtctattata acagaaaaaa
    24481 ggtacttata atacgtttaa ctgctcaaag aataactaca acatatctaa tacttaacat
    24541 tgcatgaaaa ttaaaaaatt aagactagat accctagcct taaaatgact tatgttttat
    24601 ttatcaaatc gctgataacc taaatgttca tatgccttat ccgtagctac acgaccggta
    24661 cgggtacgca taataaaacc tttttgaatt aaataaggtt cgtacatgtc ttcaacagta
    24721 tctcgttctt cagcaatatt aacggataga gttcctaaac caacaggacc tccattatac
    24781 atttcaatca tggttcttaa gattttttga tcgacgtaat caagcccctc gtgatcaaca
    24841 tctaacatcg ttaatgcttt atctgtaata ttgtcatcta tcaaaccatc tcccatgatt
    24901 tgagcataat ctcgaacacg tttcaataaa cggttagcga tacgtggcgt tccacgactg
    24961 cgacgcgcta attcagaagc agcttcataa gtaattttca tttcaaaaat gtctgctgta
    25021 cgctcaataa tttctgtcag atcattttct tcataatact ccatatgccc tgtaatacca
    25081 aagcgagcac gtaagggatt agataacata cctgcacgtg tcgttgcacc aattaaggta
    25141 aagggcggca aatctagatg aacacttcta ctagtttctc ctgcaccaat cataatgtca
    25201 atataaaagt cttccattgc actatagagc acctcttcaa ccgccatcgg catacgatga
    25261 atttcatcaa taaaaagaac atcacctggt tctaaatcat ttaaaatggc tactaaatcc
    25321 cctgattttt caattgcggg acctgatgtt tgtttgagat tgacacccaa ctcattagca
    25381 attacaaatg ccatggttgt tttccctaaa ccaggagggc caaataataa cacatgatcc
    25441 aatgactcat cacgcaattt agcagcttca ataaatattt ttagctgatc tttaacctta
    25501 tcttgtccaa tatactctct taaatactgc ggacgaagtg tacgttctac caattcttcg
    25561 tcacccattg catcactatc taaaaatctt gtcatgatat tattataaca tttgtatagg
    25621 aaaagttaag aactaagttg gtgtattagt atctccctta tttaaagaag gtaagtgata
    25681 catataattt atctacacta acaaacgata gatagggtgg actatgctaa actactttaa
    25741 aatatttaga agtcatagac cccaccaccc cattgttaac aaagttccaa caagtttaat
    25801 aactaaagtc atctgattta ctccttactt aatttgtaaa cacagtatat caatgactga
    25861 catttatcta catgacatcc ttgtcatgtt aaatctttat tccactcctc tattaatttt
    25921 ccttttaaat atagatcacc tattaactct gcaaatagaa ttgattgttt aaaacatgtt
    25981 tctgcttttg ttctatcttt ttcaataatt aataaatatt tccactctat taagcttagt
    26041 attggtcttt tttgaaaatc atggatttct gtcataatag cattacttct atgaataatt
    26101 tgctgtacat attttttctg cttaaattga aaagctaatt cgacatgatt taataaaacg
    26161 ttgttcaatt gaaaattatc ctcaggaagt aaattatctt gctctaacaa ttttgacata
    26221 ataaaatgat agttctctaa agaatacaag ctcttcattc catagttaat agaaaagcaa
    26281 agtaaatata aatctatcat aacaaaatca tttgtggtat attccttttt tttcttaatt
    26341 tgatcaaagt agtcatgtaa aatagtgatt ccaaaattat cattaacaga aaaatgaaca
    26401 tctaattttg attgcataca atcaataatt agtctttcat cttctgggag ctcctcataa
    26461 aatacttcat agatttcatc aaaatatgct tctctaactg caagtctatt agcatctccg
    26521 taagtcgggg tacgcaacaa tttgtacttc aactctttat accgtttagg taattctaag
    26581 ctctctcctc ctgttaaaat atccaatttt acattcaatt gtttagctat gtagtgagct
    26641 ttagtcaagt ttggaattga ctgtccactt tcaatcctag ctaattgtct tacagataac
    26701 tcagtttcat caccacaaaa atcttcgcga gtaatatttc tagttactct caaatttctt
    26761 actttactac caaaatgttt taacattttc ctttaaaaac taactcctaa atttttcttc
    26821 ttattatata ttatcataat aaactcattt taagtatgtt tttttaatat taattccttt
    26881 taaatcaata aaaattccat tactggttaa aaaaacgtta accagtaatg gaattttctt
    26941 atttttctaa tcctaacctt tcaaaaatat catcaacacg ttttgtgtaa taaatcggat
    27001 taaatagttc atcaagttct tcctgtgtaa gacaagaggt aactttggtg tcttcttcta
    27061 aaagtggttt gaaatccact tgattgtccc aggaataagc tgtcttaggt tgaactaagt
    27121 cataagcttc ttctcgtgtc attccttttt caatcaattt gagcataaca cgttgactat
    27181 aaatcaaacc aaaagttgat tccatattgc gcatcatatt ttctgggaag acagtcaagt
    27241 tcttaacgat attgccaaaa cggttgagca tgtagtcaat caagatagtt gtgtctggtg
    27301 tgatgatacg ctcagctgat gagtgcgaaa tatcacgctc gtgccaaagt gccacgttct
    27361 cataagccgt caccatgtga ccacgaatta cgcgcgctag ccctgtcatg ttctctgaac
    27421 cgattgggtt gcgtttgtga ggcatagcag agctaccttt ctgacctttg gcaaagaact
    27481 cttcaacttc acgttgttct gatttttgca gaccacgaat ctctgtcgcc atacgttcga
    27541 tagatgttgc aatacttgca agcactgcaa aatattctgc gtggagatca cgtggaagaa
    27601 cctgtgttga aatttcttgc ggatggatgc ctaatttgtc acagacatat tcttccacaa
    27661 aaggtgggat gttggcaaag ttaccaacag cacctgaaat cttaccagct tccactcctg
    27721 cggcagcatg ttcaaaacgc tcaatattac gtttcatctc actgtaccaa gttgccaact
    27781 taagaccgaa agtcgttggc tcagcgtgaa caccgtgggt acgacccatc atgatggtga
    27841 acttgtgctc cttagctttc tcagccacga tatttgtgaa attctcaagg tcacgacgga
    27901 taatgtcgtt agcctgcttg tagaggtaac cgtaggcagt gtccaccacg tcagtcgacg
    27961 tcaaaccgta gtgcacccac ttgcgctcct caccaagcgt ctcagaaacc gcacgagtga
    28021 aagccaccac atcgtgacgc gtgtcctgct caatctcaag aatgcggtca atgtcaaaat
    28081 ccgccttctc acgaatctta gtcacatcct ccttaggaat ttcacccaac tcagcccatg
    28141 cctcgtcagc caaaatctca acctccaacc aagcacgata tttattttct tctgtccaaa
    28201 ttgccgccat ctcagggcgt gaataacgtt ctaacatttt ttctctatcc tcttattctt
    28261 taaaaattaa tagtcacagt ggtaaaagag cttattgtta catcaaggtc atagaatgat
    28321 atttagtaac gatagtaata ggcatctgta ggaaaatctc cagggttaag cccaatagct
    28381 aagcgcggtc gtgtcgtgtc cgatgtatgc ccgttctcta tataaggatt cgatacacca
    28441 atttggaaga gataaacaga taccccaaaa ttaccactac tcatccctac agaggggagt
    28501 ctactttttt ctgtactccc gatactaatt ctttcaccat catctgtact gccatcatca
    28561 ttaatagtta aaacttcccc tttaccattt ctccaagttc ctttcaacga ggaaaagtca
    28621 cctctataaa tagcgtcaac atctaaatct ttttctaatc cagatttttt atatcttttc
    28681 caccaatccg gagattgata ggaaagagaa attaattgat tattagctcc tacttcaaaa
    28741 acttgtcttt ttgataatac ttttccatct ggcccctcca cttttggggg aactaaatag
    28801 aatatacgtt tcaaactacc tgtactattt atcgctccag aattagcgcc atacacatgt
    28861 atttcataat aatttttaat tttcttgggt aaatcatcgt tttttataac ataactacca
    28921 tgagacgtta tgccattcca atagcctgtc ttcgttattg caggaaaaac tgttgttcgc
    28981 ccctcatagt agtctttcgg ttcatatccc ccataagtca tacccgaatc tacaatagtt
    29041 tggttaaaac tatcacttga aaattgaccc ataaatgggg ttaaatctcc ttccaaaacc
    29101 ttatcataag aatggttggt agtagattca tctctattta acatattatt agcaaatttc
    29161 ttcttcgaaa actgcatagt atgctctggg ttatcatcaa acattttgat tttcacattt
    29221 gaagtacctt tataataaat ttggatagaa aaattgttat taccatctga ttttgtcatt
    29281 tttgaagttg agatatagca atgggaacct gtttttttaa acattggacg aaaagtcgca
    29341 acaacattcc cgtctagtgt tttgtaagtc atggaccatg cgccgctttt tttcctctga
    29401 attttagcct tttcaccaga agccaaatcg taataaatac ctcctaaata aaaatgtttt
    29461 tttgagaaga agatttatat tcttgacttc tagtactact aattttacag gaatataatg
    29521 gaaaaacaca agtaacaagc aaacctaaaa taactaatct attttttaac aaccttatca
    29581 cctaacttat tctatataaa aaatcaaaag ccctttcgct ccaaagaaga taaattcgta
    29641 cttttaataa aattgaaata ttgaacttga ttattatcta ttttcattca ctcttattta
    29701 ggaataaaaa gcatctgtct atctattttt aacttcctag gaatatctcc ttaattacat
    29761 tctccaacga gcagtgaatt ttaatttaag actagctatt ctcattagtt tcattagcct
    29821 tttcttcatg aaagtcctct ttactaattg ctactgatgg gtagtgggac agtttattta
    29881 aatctgtatc taatggtaaa tcataaatag ctaaataatc acctgggtag tggcgctgaa
    29941 attctgacat tttagtcatc aagagtttat catctgatgt cgcaaataat acttcaacaa
    30001 cacctatttt accactatta ataaaagcat gaacaatgat tttaatcata caaacctcct
    30061 aaaactcctc cacctcatcc gcatcttccg taaacaccgt cacatgtccc atcttgcggt
    30121 tatgtttcgc ttctagttta ccatatatgt ggaggtgagc gctagggttt tcggcaacat
    30181 agtcaatcgc ctgctggaca tgttgtccaa ggacattaag catgacggct ggggcatgga
    30241 gcttgattgg tgggagaggt tgccccagca cacccaagat atgagtgtca aactgtgaaa
    30301 agtcacaggc ttcgatagaa tagtgccctg agttgtgggg gcgtggggcg atttcattga
    30361 cgatgatatc atctgcggtc acaaacattt ccacacagag ggttcctgat agctggagtt
    30421 ccttggcaat ctgtaccgcc attttcttag ccttgtcagc tagttggtct gagatgcgag
    30481 ctggtacgat agtttttgat aggatattgt tgcggtggat attttcctga acgggaaaga
    30541 ccgtcacatc ctgaccattt ccagacacga tgactgagat ttcaagatca aagtcgacaa
    30601 actcttccaa gacacactcg gctgagttgg ctaattgctg cgcctctggc aggtcttctg
    30661 ctgagcggat aaccttttgc ccatgaccat cataaccacc tgtcgctgtc ttgaggacat
    30721 aggtcttggt caagtcaaac ccctctaggt caaggctaga cgtcaccacc ttgtaaggag
    30781 cgacagtcac accagcctta tttgccagaa agtctttttc aaagatacgg ttttgagaaa
    30841 tgcggagcaa atcagtcccc tgcggtagct gaccagccga tacaacggca tccagaccgt
    30901 cggcatcaac attctcaaac tcataggtca aaacatcgca acgcgcagct aattgtccca
    30961 aagcctcaac atcatcataa ggcgctacaa tcacttcgct aacgcgggag gcagagcagt
    31021 cactagcagg atccagcgtg atgaccttgt ggcccatgta gatagccgca attgccatca
    31081 tctgccccag ctgaccacca ccgataatcc caatggtctt aaataagttc attgcttgac
    31141 tcctccgcga tttttccttg ttcctcatgg aaatgagcta gcgcatcagc cagattttgg
    31201 tcctcaatag agaggatacg gagggctgtc aaggcagcgt tagtcgctcc cgcttctccg
    31261 atagccatgg tcgccacagg cacaccacca ggcatctgca caatagagta gagcgaatcc
    31321 aaacctgaaa gggcacgtgt tttgaccggc acaccaataa caggtagtgt tgtcttagct
    31381 gccaccatac caggcaagtg agctgcccca cctgctccag cgatgatgat tttgatacca
    31441 cggccacgtg cttcctcagc atgcttgaac atgaggtctg gcgcacggtg ggcagagacg
    31501 actttcttct cgtaagcaat gccaaagtta tctagaactt cagccgtttt ttgcatggtt
    31561 ccccagtcgg atttggagcc catgataata gaaataattg gagttttcat aaataagata
    31621 caaagggacg tttcgacttg tcgaaaattt ccgtagaaaa atatgcggta agtccgaaat
    31681 ctttgatttc gaagatgcac ccacgtcagg gcgactaggt gctttagcac cagacgccta
    31741 cgacgcagaa atctaacgat gacgcttgcg ttcaggtagt tttatttttt tcctaggaaa
    31801 ttagtcccgt gttcagttcc tttcttcttt ctttttggag cgaccttcgg tcgctgattg
    31861 ccgacgctaa tatttgaatg agtagtctta ttacttaata gctttgcttc cgatatcatt
    31921 tcggtagaag aggcctgttg tgtcttgttg ggctagttgg gtatagattt tgtcctgccc
    31981 tgctttgacg ctaccttctg tggtgacgag catatagaca cgtccaccgt ttgagagcag
    32041 tgctttgcta ttttccgcaa acttagctcc tgcatagtag gtgatgatgt cgccgtcggt
    32101 cttttcaggc agtggcacac ctttctcgta atcgagcgga tagccttctg aggcaacgac
    32161 aacgcccaaa gtcacgccgt ccttctgcca agtgatgtaa ggctcaatgc ccatcatgat
    32221 gtcgtcgatg ttctgagcaa aatcggaagt taggcgaggg aggataatct gagtttcagg
    32281 gtcaccgaaa cgtgagttga actcgataac cttagggcca tcagctgtca ggataagccc
    32341 cgcatagagg acacctagat aaggacgccc ttcagcaatc atgccctcaa ggacaggctt
    32401 aatgatggtc tcaaccgctg tatccaccac gctctgaggc agatgaggaa caggcgcata
    32461 ggcacccata ccgccagtgt ttggtccctt gtctccgtca aaagcacgct tgtgatcctg
    32521 agctgtcggc atgatgtaga acttatcgcc attagcgaag gcaaagaggg agaactcttc
    32581 accatccaag aactcctcga taaccacgcg cgctcctgag tcgccaaact tgttgtctaa
    32641 aagcatctct tgcgccgcct ctaccgcctg ttcaacggtt tcagccacga ccacgccctt
    32701 acctaacgcc aagccgtcag ccttgaccac gattggtgcg ccctgctctt cgatgtaggc
    32761 tttagctttt tcaaaatctg aaaatgtgcc ataggctgct gttggaacat tgtatttgac
    32821 catgatttcc ttggcaaagt cttttgacca ctctagctcc gctgctgcct tggttggacc
    32881 aaaagctctg agtccagcac tgttgaaatc atcaacgata ccagccgcta gcgcatcatc
    32941 aggaccaata aaggtccaag cgatctcatt ctccttagca aagtcaatta gcctagaatg
    33001 ttcggaaatt ccgatattta ccaagtctag accatccaag gtcataccat cattaccagg
    33061 tgccacaaaa acctgatcca catccttaga cgctaacaac ttcttagcaa tcgcatgctc
    33121 acgaccacca gaaccaacaa caagcaattt catatcagaa taactcctat taaataatat
    33181 aaaaagaaat ttgcaacaaa aacgtattac ttataaaatt ataacacatt tgttcgcttt
    33241 taagtttttt tattacatct tttttgaaaa attaataaca taatatatac ttcacatata
    33301 ttatgttatt aatccttcat attgctattt aaaacttatc attgctgttt cttagaactt
    33361 agtttggtcc ctcgctaact ttatgttata ctgaatatat taagcgcttt cttaaagagg
    33421 agaggaccgt gtattatgac tatttatgac caaattgagt ctgctcttga tttaatgact
    33481 gatttagaac gtgaaattgc ttgttatttt atggggcaac ccatttcaaa agacgctctt
    33541 gcttctacca ttgtcactaa gcaactacac atttcacaag cagcattaac gcgttttgct
    33601 aaaaaatgtg ggtttaaagg ctatcgtgaa tttgtttttg aatatttaaa aagccatgag
    33661 accatcagtc aacaattata cggtcttcaa aatgacaaca ctaagaaagt atttatgaat
    33721 tatcaagaga tggtttctaa atccgctgat attattgatg aagaacagct tctagaagta
    33781 tcacatatga ttgagcaagc tgatcgtgtt tatttttatg gcaaagggag ctcatcgcta
    33841 gtagctaaag aatttaaaat acgccttatg aggctaggag tgatttgtga ggctctggat
    33901 gatacagata gtttctcatg gactaatagt attgttaatg atagatgcct cgtcattgcc
    33961 ttctcacttt ctgggaatac caactctgtt atcggtgcct taaaaatcgc aagtagtcac
    34021 ggagctaaaa ccgttctatt tacaaaacaa cctcacacaa ttgactatgc ttttgacaag
    34081 attattcagg tggcttctgc taggcattta gactacggca atagaatttc gcctcagatc
    34141 ccaatgttaa tcatggtaga tattatctat gcacaatttt tagatattaa taaaatagaa
    34201 aaagagcgaa tttttagaga aaccattatt caacgctaaa tgaaaatcga gtagaaataa
    34261 tctactcgat tttttgcttt gtaaattgac ttcctgtcag ttgatcaaaa atatagtctt
    34321 ttacttgcac tgtcattgga tcatgtccat aatctggtaa taaaagatgc tttttagttg
    34381 atgtcagacg attaaaaatg gcaaattgag ttgatggagg acaaatatca tctttcaagg
    34441 ctgttagtaa aacaacgggg caagaaatcc tatgtgcaaa atttttaaca tcaatataag
    34501 ccaaagtttt taacacacta ttctctgttt tatgaaaagg atcactatat ttaaaatagc
    34561 ggaagagttc atcatagggc tcactgacac cccctaaatc taatactcgt ctaaagtctg
    34621 acaaaaatgg gtaaacagca acggtcttga cgattttggg attaagagct gcagctatca
    34681 aagcaagtgc tccaccttga gaccagccat aactataaag ttgattacta tcaacactct
    34741 ctaatgtagc aatgatatca atcaattgga aaacatcaag ataaatgtct ttataaaaaa
    34801 gatgatttgg tcctgagatc attcctcgca caatttggcc cttaactgta atgccatcaa
    34861 aatgcccctt gtcttgagac tgtccagctt gacctcgaac atccattgaa acaacgccgt
    34921 aacctgctgc tacataatta agttggtcac tccagtcagg actttggttt tggtagccat
    34981 gaaaataaaa aactacagga tagggtttat ttgttttagg gaataaacat ttggcataga
    35041 caatagagtc attgctacct ttaaaatgaa gatcataagc ttgtgcaaaa gtaatattga
    35101 aatctttttt gtctagacga tactcaatat tcccttgata tttcattgtt tgttttttcc
    35161 agaagtcatc aaaatcctcg ggaatttggt cttgacctag gtattctctc atgtcatcta
    35221 gcgacattgt ttcaatcata tacccgtcac cccctgtttt taaaatggta gtaagctcct
    35281 agcataccag cttggttttc gtgttgtgca aaaactattg ctgttttttc agcaagactt
    35341 gtcacaagat ttcgtttcaa agattcacta agtttatctt gtaaataatc tttttgagcc
    35401 atgattccac cacctaaaac aactttttca ggatttacca cataaaccat attagcgatt
    35461 ccttgaccaa ggtagtttat catacggtct atacttgcaa tacatttctc attaccttct
    35521 ttagcctcct gaaaaatacg acgtccatcc catcggctaa tttcatctcc atgcgctttg
    35581 gcaacatcag caatcagtgc agttgtagag gctaaatctt gaaaatcacc atctgaaagg
    35641 tgcatatatc caacttcaca agcagaatta ctaaagccat gaaaaactgt tttatcaata
    35701 atcagacaac caccaatacc tgttccaata gtaagacaaa gcgctattga actatctttg
    35761 gcactacctg agacagcttc agctaatcct gcacaattaa catcattttc aatttctgtg
    35821 cgtacttgat aagtgtcttc taaaactttc ttaaattgtg tcccagcata gtttggaatt
    35881 tgaggaccag agtaaaatat gcatccttca tctggatcta ccatgccagc agatgagata
    35941 gcaattccat caatgctccc ttctgttaga tagttatcga taatttgaca aactttttgt
    36001 aaaatgcctg gaccaccttt gtaggcttct gtggcaagct cacttgcctc aacaatacaa
    36061 cctaaattat ccacaatgcc atgcttaatc attgtaccgc caatatcaat tgctactgtt
    36121 ctggtcataa ttatctacct cactttaaca aaactgtttt ttagcatgtc taatcatttc
    36181 tgctgcctct ttaataatag gtttatcttc ttctgttaca gatgctaaag gtaaccgaac
    36241 ggaacctata gttagttgct cattgatttc caatacagat ttaatcacag catacatatt
    36301 accatgacct gaacaaagct ttgtgataat atcgttaatt gtaaattgaa gttcacgcgc
    36361 tttttctaaa tctttatcaa caattaactg atttaaagtc aagtataatt ctggcattac
    36421 gccataggta ccaccaatac ctgcagctgc tcccatgaga cgaccaccca aaaattgttc
    36481 atctggacca ttaaaaacaa tatgattttc tcctccaata gccacaaaat tctgaatgtc
    36541 ttgaactggc atggaagaat ttttaacacc aattacttgc ggattttgta acatttttcg
    36601 atataaatcc gatgtcaaag caacccctgc taattgagga atattgtaaa taataaaatc
    36661 agtttgtggt gctgcttgac taatagtatt ccaataatct gctatggcat actctggtaa
    36721 tcgaaaataa attggtggaa ttgctgcaat agcatcaaca cctatagctt cagcatgcat
    36781 agccaactct acgctatcct tagtattatt acatgccaca tgcgctatta ctgttagttt
    36841 acccttagca acggacataa cattttccag tacaagttta cgatctgcta cactttggta
    36901 aatacattct cctgatgaac cattaacata aagtccttga acacctttat caataaaata
    36961 attggtcagg gcttttactc gttcgggaca aatatcaccc ttatcatcat agcaagcata
    37021 aaatgcagga ataattcctt gatatttttg taaatctttc attgataatc tccttgttgt
    37081 ttgctgaaat aggcaaattg tttttgtaaa ataataattt taaagtaagc aaaggcggta
    37141 taagcaaaaa taaatatagc tgacactgta agcataaata agagactaga ctgcaccata
    37201 ataaaccaag tcatgattaa taaaaacatc aatacaccag tccatttcaa gtttaaacat
    37261 gccattataa agctcttttt cacggtatct aaaagagaca tttcgtagcg ggcagccata
    37321 ggaaataggt atatgctcgt catcacagtc aataacaaaa aggcgtaaca agttatcttc
    37381 aagaaagaca caatgaaacc tatttgataa tgaagaattg ttaaatcaaa tatgatcaca
    37441 accaaaatac ttagttcaac caaacctaaa agcatacctt ggaaccactc tttttttagg
    37501 tgttttatat acaatattaa aagatttgtc tgatcacctt ctaatttttg ccacaacgtt
    37561 cgatacaaac taattttagc agcaccaata gttaccaatg gtaggcaagt tatcataaaa
    37621 agaaggttta gtaatgctag atcaaaaaca acattgcaac cttgcataaa ggtgttgtta
    37681 acatcaaaaa tcgctgctat taattgattt gcttttttca caatatactc cctgtatttc
    37741 ttacgccatt aacactttca ataccccttt tttgacataa ttacctagac ttcccatacc
    37801 atttggctga tgagcttctt ctgggaaaaa gaaggctata taattatctc tcaaatcaaa
    37861 agaagtctct tctgagcact tgataaatcc tatatcgcct aagtattcct ctacctctac
    37921 cttatcacct tttcccaatt tgataacttc atgtccttca atgacataat gtatatcaag
    37981 gtatttttta tggtattcaa aaatatgatc aaatgactca ctaagttgat ttgattggac
    38041 catgagtatc acttctgggc taatatgata ggtgcctata tctaaattcc ttaaatcatg
    38101 acttagtaga taatcaatcg ctaaatctaa attaggatta atatctttat aatgcgttaa
    38161 attcaataaa tgatcgtaaa tcataataaa tcctacctat cctttaacag ctcccattgt
    38221 aatcccctga gtaaaggatt tttggaaaac taagaatact gtcacaatag gaacagcagc
    38281 taaggctgca cctgccataa ttaagccata gtttgttgcc atttcagcct gcatagtagc
    38341 taccccaaga gaaatggtca gattatttct agaagttagc ataactaatt gcataaaata
    38401 gtcattccaa gtattgataa atgtgaaaat agctagggca gcaaatccag gtttgacaat
    38461 tggaaatgct acattaataa atgttcgaat ctctccacaa ccatcaattt tagctgattc
    38521 cagtaattct gttggaatat tttcagaaaa ctgtttcatc aagaagaccc caaaaggcca
    38581 accaacaaga ggtaaaataa ccgcccataa ggtatcatgt atccccataa aattgataat
    38641 tctaactaat ggcactaaaa caacttgttt aggtaaagcc atagcagcaa taaaaaggga
    38701 gaacaatatt ttttgaccgt aaaatcgttt cttagctaaa acatagcctg ccattgacga
    38761 tgtacagcaa actaggaaca ttgtcataat tgagataaag acactattcc aaagccatct
    38821 caaagctggg ttttgtacag ttaaagcttt aaaattttct aaggtcggtg ctttgggcca
    38881 ccattgtggg gggatgataa tagtatcagg ttgagattta aaagctcctg tcataatcca
    38941 ataaaatgga aagataaaaa gcactgtcaa aagacaaagg ataagagccg ttaaaaagtt
    39001 ataagcactg aatgttttct ttttcatgtt ttcctcctaa tattccacat catttcctaa
    39061 gatcttaaat tgagcaaaac ttattaaagc aatcattact gctaggaata ctcccatagt
    39121 attagcatag ccatattctg atagtttaaa ggctttttca taaagataat acatcaaagt
    39181 gcttgttgaa taatttggtc caccagatgt taggagctgg attaaggcaa aacattgaaa
    39241 tgaattaatt gttgttataa ctgcaatata gagtgtagtt ggtaagagac tcggccattt
    39301 aatttgccaa aaaacttgca tttcattggc tccatcaaca cgcgcagctt cgcaaagaga
    39361 attatcaata tttcccatcg ccgcaatata tagaataata ggttgaccaa ctgaagtcgt
    39421 tagcaaaata ataataatag caagtaatgc ccagtgctta tcaccaagcc agctaatatt
    39481 ctgttcaatg acatggcctg atttcaaaat gtaatttaaa attccggaca tagggtcata
    39541 aatccatttc caaacaactg tgactgcaac actaccagtt actactggca agaagaagac
    39601 acaacggtag aaagagcgtg aaaaaacatt tttttcataa gtattggcag caacaaaaag
    39661 tgagaagaaa accactactg gcacagagcc aatcacaatg attactgtat ttatcaagga
    39721 tttcatgaaa atagaatcat gaaacatacg attgtaatta gctaacccaa taaaagtgaa
    39781 ctgtgtcata gaataattaa aaaaactagt gacaaagccc ataaccatcg gtgcaaaaac
    39841 aaaaattaag aaaaataaga gaataggcgc tagaaaagca taagctatca tcgtttccct
    39901 catctttaac ttattagtac gcatacggta ctctccttta catcttaact tgactaaaaa
    39961 ctcctcttct atcccaatat gtgattgacc atagaaaagg agtctcctta attatttagc
    40021 tgctttttta atggtatcat ttgctttttg agtaaagtct ttcaaagcat ctgccggttt
    40081 ttcatcacca ttggatacag attgaaccat tgggaaccat aaggttctca tttcagaaaa
    40141 tccatcgata gtgttgtaat atggtgaata atattgagtc cattttgaaa tcttcatcat
    40201 acgtttatca cctttataaa gatccccaaa tgatgttcta actgggaaag cacctgtacg
    40261 tataacatct tttggtcccc attttttatc gtcagcaata aaagtgataa atttcttaga
    40321 ggcttttact ttgttttcat ctttattatt aaagaccgca aaaccattaa caaggtattc
    40381 taaatctggt tttccatctt ctgatgggaa tggcacttca aggtaatcca ctttacttga
    40441 ctctaataat tttgcttgag tttttggttg agctggagcc cataggatag tgaaagcagt
    40501 ttgtccatta gcaaagtttt gaatgtcagc tgagccatca tactgagacc cattcatcaa
    40561 gtagcctttc ttaatccatt caactatttt tttcattgat tttacagaat ttttagtgtc
    40621 agtggtatat tttgttactt ctttatctgt tattggagca ctataaagat tagcaaaaaa
    40681 tgcacgcggt ccttgatctc ctccttgccc gtttgcaaag aatgaacctg gtgtatagcc
    40741 tttatttttt agtgctttta gtactttttc aaaatcacta gtagtccaac cttcttttac
    40801 aagtttcaaa actcctgcat ctttaagcat ttttttattg aacgccatat aaaatggggc
    40861 agaacttatt ggatacatgt aggctttatc gccagactta gaagcttgaa tgatgttctt
    40921 attattgaca tccttaataa attggtctgt aaataaatca ttcaaatctg ctaatttacc
    40981 atttttacca tattgaataa ttcgccctgg tgcatcaaaa agcacatcag gtgctgtccc
    41041 tgcttcaatt gctgtagtga ttttttcagg tccagatgtg aaatcaattg tctctagttt
    41101 tacttttata ttaggatttt tcttttcaaa agccttgatg acttttttct cataagtacc
    41161 tactccatcc ttagcctttt cttgagtaaa ggttggaaaa gcccaccacg taatttcgac
    41221 tttatccttc tcggcagcca taacagattg actactgtct acacaagcaa acattgataa
    41281 tgctgcagct ccgaggcaaa aaccaatcac actttttttg atactcataa ctattcccct
    41341 tttttatttt gattttgatt tttgagtaac acttttaact aagtcctgag atgaaacgct
    41401 ccgctatttc ttttggtcta gtgatagcac caccaactac aattcctgca acacctatat
    41461 gattaatttc attagcttgc ttaggagtat gaattttacc ttccgcaatc acatctatac
    41521 cggcttgaca aagcttatta aggagttcta tatccggtcc ttcttcttgg cggctgtaat
    41581 ctgtgtatcc agatagagtt gtacccacaa aatcaactcc tgcttcaaaa gcatttttac
    41641 cttcttcaaa agtacttata tcagccatta gcaactgttc aggatatttc ccttttatct
    41701 tttgaataaa ctcagctaca ctcaaaccat catgacgctc tctaagtgta caatctaagg
    41761 ctattactgc aatatctaaa ctagctaatt gatccacctc tgtcatcgta gcagtgataa
    41821 atggttcttg tggaggatat tcacgtttaa taatgccgat gataggtaaa ttagtaactt
    41881 cttgaatttc cttaatgtcg cggacactat tggctcttat accaaccgct cctgcttctt
    41941 gagctgccaa agctaaaaga ggcataacac ctccactttc agtataaaga ggctccccag
    42001 gcaaagcttg acatgacaca ataatgccat tttttatttg ctttttaaaa gcttctttac
    42061 ttagatgtgg catattctca cttccttatc ttttttaatt ttagatatct acaaacctta
    42121 ttatatctgt ttttaaagcg ttttcaataa tctttacaga cttagatttt agttttaacg
    42181 ttttgattta gtcacggtta acgtgaaagt agtttcagac ttatagctta atctcctcaa
    42241 tttaataaaa attagataag aaccttgaaa gtctattcaa ggcatataaa aaagaagcca
    42301 aatagcttcc ttttttatat tatttgttaa atgatacgtg aacatggtca taatggttgg
    42361 cagtaacgcc accacgatct ggcattgcat tccaagtatt agcaggtcca taaatactat
    42421 ttgtatttga gtaaaacttt tgttgccaga taacatatga aatgttattt gctgccatat
    42481 tttgtgtaga gtactgtgca acttcattac caagtgcttg gtttttacct acaataaagt
    42541 cgactgctaa acctttacca tgatcacctg gatcacctgc acggtatgta ctgaattcat
    42601 taactccata agttgacgct actttttctt tataagctgc aacatgaggt tggagccctg
    42661 cattttcagg atgtgcagct actgcatttg ttgttgaagc tggttgtgct accggtgttg
    42721 ctgttggagc tttttgtgct accggaacgc tcttaacttc agtcgcttgt aacttactgt
    42781 ctgtagctgt tgaagtcgta gtcacaggaa ctgctggagc tgatacatgc tctggtgatg
    42841 caccagtttc tactttagga gtgactactt taacacttgc cactctaggg gctgctacag
    42901 ttcttaccgg tgctacttta gctactggag ctggtgtttc agcggcaaca gaagctggtg
    42961 atactgttgt tgactgactg actgacgtct gagttggttt aacttcctct ttagctgctg
    43021 gaacttctga agtaatcgac ttcacaggag ctgttgatac ctgttcatta gctgctgctt
    43081 gactaacagc ttgctcttgt gctaatactt cttttgattt caaagctggc gcagaagaat
    43141 atgtcttcat tggcgaaaca atcgttgttg ctgcttctgg tgtcatacct tccgaaattg
    43201 tattgagaga aactttttgg tctgcaacag aaacttgatt ggttttcaaa tccacagtag
    43261 ctgttgtttg accagcagca tttgttgctg gtgtttctat tttcattgaa gtggcagtat
    43321 gactcttctg atcgtaagtt actgtcagtg ttgtctcagg ataaataaga ttgatatctg
    43381 caatgttatt aatttttgct aagacattca tatcaattga cattgcttct gaaataacgc
    43441 ttagtgtatc accatatttc acagtatatg atgatttatt gtcttgcttt accaaatcag
    43501 cctttacctc tgaaacagta cgtgctgtcc acgtcgtatc tgtttcttgt gcttgaacac
    43561 ttgcgactga taatagcgaa gctgccattg tcgatgtcaa tagtaccttt ttattcattt
    43621 tcattgcttc aattcctatt tcctttttat tagtccgaga ctataatatc acactttact
    43681 ggactataaa attattttca gacggtttaa agaaattgta cttagttagt aacaaaactg
    43741 tcatttaaaa caatgaatca atacctcaaa ctatttgtaa agcagttctt gactgttgtc
    43801 aacgcgaagc caaactgaac taccattagc aaagcgtgtc ttagcccaag ttaaaccagc
    43861 tgttccttgg atgggttttt ctacagtctt aagagtttta ggattaaaga taaagtaacc
    43921 acctttttga agaacctggt cagctgtcaa attaccatta gcatcaacct catcaatttc
    43981 tgatgctggt ataccattat cgttccaatc aaacccagtc ggcgttaggg tgttattttt
    44041 gactaaccaa acaccattaa ccttttgtaa ttcatctact cgatacactt ttaatttact
    44101 agattgattc tgtacaggag ctgattgtaa tggcttaatg cttggagctg atgcttgcgt
    44161 ttttccacta aaggtcgcaa cgttagcaat tagtgacgtt ggattgatac gtccatggaa
    44221 accattttga aaattagggt tagctggtaa aaattcaaaa tgaaggtgag gtcccgtcgc
    44281 cataccagtt gctcctacgt aaccgatgat atctccttgt ttgacttttt ccccagtcct
    44341 agccaccaca cgtgacatat gagcgtaacc actatgcatt ccatccgcat gttgaatcat
    44401 gacacaattt cctgctaagt ctgtcatcca agaaaagttg gctccagctc ctgcaaattt
    44461 cacagtacca tctgccactg ccctaataat cgttccagtc ggaacagcat aatccacccc
    44521 acaatgtcca ggataaccat tgaaacctgt tgtaattcta ccattatcaa ttggacggac
    44581 ataagtatcc gctaaaactc gggaacccgc agataaaacc ataccaccta aaacaactaa
    44641 ggaacttgcc ttaactaacc atttattcaa gaaaaacctc caaatagaat taatataatg
    44701 aagtgctcaa acacttgcaa ttaacttatt cgaaaattta aatcaaaata tcaaaaaaag
    44761 tactcacctt gatgagtact taggaggttt aataaaaaga aaagttttta ggatatacgt
    44821 agtatacctg agttaactta agaaaaccta aactactatt ttttaatgtc taaaatgtct
    44881 cacgcctgtg aagatcatgg tcaagccatg tttgtttgcg gcgtcaatag attcttggtc
    44941 acgaactgaa ccacctggtt ggatgattgc tttgatccct gcggcagcga tttcttcaat
    45001 gttgtccgca aatgggaaga aggcatctga tgctagaacg gcaccgtcaa ggtggtcctt
    45061 agcctgctcg atagcaatct tgactgagcc gacacggttg gtttgacctg caccgagtcc
    45121 aagcgtcatg tgatcgttag taataataat cccgttagac ttaacatact tgatagcctt
    45181 ccaggaaaac tcaagggcag tcgcctcttg ttctgttggc tggcggtctg tcaccacttg
    45241 ccagtcagat ggattttcag ccacaacgtc ttggttttgc accaaaagtc caccaactac
    45301 gccagtgtac tcagcttcca cttcgctggc agcttgggca tcaaacggca actcaaggat
    45361 acgcaagttt ttctttttat ttgtgagaat agctagcgct tcttctgagt atgatggtgc
    45421 gatgatgatt tccaagaaga tagggtgcat cttctcagct gtcgctgcgt caacttcacg
    45481 gttaaggaca acaattccac caaagattga aactgggtca gcttcataag cgtaatccca
    45541 agctgtctca atatcatcag cttgtccgat accacatggg ttcatgtgtt tgagggcaac
    45601 aaccgttgga ctgtctttga aatcgcggat aatacggatt gctgcatcag catcacggat
    45661 attattgaag gacaattctt ttccattgag ctgtttagct gaagcgattg agtagtctgt
    45721 tggtaaggct ttttggtaga aatcagcgtc ttgttgtgga ttttctccgt aacgcatagc
    45781 ctgtttaagg tcataagtga tggtcaattt ttcaggctta gcctctccca cttgagctgt
    45841 gaagtactca gcaatcaaag cgtcatagga tgccgtatga cggaagacct tagctgccaa
    45901 gcgttgacga gtttcaaatg tcgtctgacc agcgtcagcc aattctccca aaacagtggc
    45961 atagtccgct gggtcaacca caacggttac gctagcgtgg tttttagcgg ctgagcgaag
    46021 cattgatgga ccgccgatat cgatgttttc caccgccaaa tcgtaggtca cgtctgggcg
    46081 aaggatggtc tccttgaagg gatagaggtt gacaaccacg aggtcaatca actcaatatt
    46141 gttgtcctta gcagcctgaa ggtggctgtc agcgtcgcga cgagccagaa gcccaccgtg
    46201 aatgtttggg tggagggtct taacacgacc gtccatcatt tctgggaatc cagtcacatc
    46261 gtcgatggta atggtctcaa caccagcatc gtcaagggca accttagtcc caccagttga
    46321 gataatatcc caacccaagt ttttcaattc ttttgcaaag tcaataattc ctgacttatc
    46381 agaaactgag attaaagcac gttttcttgt cattgttttt ccttttatca attaaatttt
    46441 cgttccagct gaatctacat accaaaaatg ctcgctattt cttttacatt ctaaagccgg
    46501 cactctcttg gtaattttga tctttttgat atttccattt tcgtcataat gattaatcca
    46561 atcatatggc ttattgtagt atttcaattt tccaccacat tttggacaag gagttatatg
    46621 caacttttca atattcaatc ttttacctac accactgatt gcatagttaa aacccaaagg
    46681 atagcgagtt tgactcttag taattcttcg caaagaaaaa ataacaactg aaaaaataaa
    46741 caaacctaaa aaaataaaca aataaatttg actaccctgg acattgggaa tatttccatc
    46801 aaaagaattt ttagcccaac ttataatcgg ttttaaaaaa ttataaaggg gtaacaaact
    46861 tcctatcgaa gtgaagaagc caatccaact caacactgcc attgtaatcg gacttctcca
    46921 aataggttca acatcatatt tagcgactgg ttgctctgct tgagaattag taatattaat
    46981 tatttgatct ctaccagctg aattgcctga accaatatta tgtcctccat taaaactatt
    47041 attatttgtc atattctttt ctactctcct ttatttagta ttccattctt tcctctccac
    47101 tcccaagcta tctaaaacag ctggatagag ttgatattca gtttcgtgga tacgggtttc
    47161 aaagctttct aggctatcat ctgctaggcg tggcacgcgc acttgttgga tgacctgacc
    47221 ggtatccaca ccagagtcca cccaatggat ggtcacgcca gactggtcaa caccagcttc
    47281 ccaagcatcc tcgataccgt gggtacctgg aaattcaggc aggtaggctg ggtgaatatt
    47341 gatgatacgg ccctcatagg ctgagagtaa ggtttctcca acaatcttca tatagcccgc
    47401 cagacagacc aaatcaatct cgtgtttgtc caacaaatca acaatggctt gctcgtaagc
    47461 cgtcttattc tcaaactcct tgagttcaaa agcgaaactt ggaatggcta agttctgagc
    47521 acgctctaaa acataggcat cacgatgatc tgaaaagaca aaactaactt gaaactgctc
    47581 tgctatgacc tgaaagttgg aaccattacc agaagcaaaa acagcgattt tcatattcga
    47641 tacaaaggga tgattcggct tgccgaaaaa tttcgtagaa aaataggaaa acgaacaacg
    47701 acgcttgcgt ctaagtcgtt ttatcttttt tcctagaaat ttaatcccgt gttcagttag
    47761 gcaaactaac taaacactcc tttcttataa aatttagtga cttatttaat caccacacta
    47821 tcgtctgctt tcttgatgat acgaccgatt tcatagactg gttcgtccaa aagttctttg
    47881 acacggtcaa cattttcagg gttaacatcc agcataagac cgacacccat gttgaagatt
    47941 tcaaacattt cttcgtgctt gatgtcacca tatttttcaa gcgccttgaa aatcggaagt
    48001 acaggcacct tatcctcatc gatttccgca gccaaatcat ccgcaaacat acgaggaaca
    48061 ttctcgataa aaccaccacc cgtgatgtgg gcgataccgt taactagttc ttccttgatc
    48121 aatggcagag ctgctttaac atagatacga gttggctcaa gaaggacatc cttgagttgt
    48181 ttgccttcaa gctctggaag cacctcatca ccagtgtagt cagcaaagac acgacgtgcc
    48241 aatgaataac catttgaatg gataccactt gaagcaagtc caagaagaat atccccttct
    48301 tttacctttg aaccgtcgat gatttgagat ttttcagcca caccaacagc aaagcctgca
    48361 aggtcataat catcttcgcc atacatacca ggcatctcag ccgtttcacc accgataaga
    48421 gctgcgctag cttgaacaca accttcagca acaccagcga caacctgttc caatttggca
    48481 ggttcgtttt taccagtcgc gacatagtca aggaagtaaa ggggctcagc acctgctgca
    48541 ataatatcgt tgacacacat ggcaacacag tcttgaccga ttgtgtcgtg cttgtcgtac
    48601 ttgatagcaa gcatgagttt agttccgaca ccgtcagtcc ctgaaatcaa gacaggttct
    48661 ttaacacctg tttgtgacag gtcaaacata ccaccaaagc caccgagagc tcccatgaca
    48721 ccagcacgtt ctgtgcgagc aacgtgtttc ttgatacgtt cgacaacttc gtagcccgct
    48781 tctacatcaa caccagactg ggcataagca tttttttcag acatgttttc tccttatata
    48841 attagcagag cttatttcac tttttgaata taaaaacttg ttttttcttc taaacttctg
    48901 aggtattctt cttcataatc ataaagcggt gttggataat gtccatcaaa gtaagctacg
    48961 cataaaccac catttggtgc ttttgtttcg agtccaattg atttaattaa cccatcgatt
    49021 gatagataag ttagactatc tgcgccaata atatcacaca cttcgtcaac agcgtgattt
    49081 gctgaaatta attctcgtct tgtttgaata tcaattccat aaaaacaagg atactttaat
    49141 tctggactag ctatagcaac atgtacttca gtagctcctg cttctcttaa taatcctaca
    49201 atccttctag aagtcgttcc tcttacaatt gagtcatcaa tcataacaac gcgctttcct
    49261 ttgacaacac cagataccgc tgataatttc attcgaacac cttgttccct taattcttgt
    49321 gtcggttgaa taaaggttcg ctgcgtatac tgatttttta caaggcccat ctcatttggt
    49381 aatccggatt cttcagcaaa gcccatagca gccgataagg acgaatttgg gacaccaatt
    49441 acaatatcag catcctgttt aaattcttgt gcaagacgct ttcccatatt ttttcgagcc
    49501 gtatgaacat taacaccatg tatagttgaa tccggccttg caaaatagac atattccatc
    49561 gaacaaattg ccagttgcgt ttcatcagta taacgatcac attgaatacc actatcgtct
    49621 ataagaataa cttcaccagg ttcaacatct ctaacccatt ttgcgcctac cacctcaaaa
    49681 gcacaggtct cactggaaat aacccaggca ccattttgca tttgtccaat tgacaaagga
    49741 cgaaaggcat tagggtcaag agcagcaatt aatttatctt ctgtcatcag tagataggcg
    49801 aaacctccct ttacagtgct taaagcttct tttaccttcc ccataaaact tgggttatgg
    49861 cttcgacgaa tcaaatgcat taaaatttca gtatctgagg aggcattgaa aattgcacct
    49921 tgcttttcta attctttcct tgaggaaata gcatttgtca aattaccatt atgacataaa
    49981 gcaaattgcc catcatgaaa tttataaaga aaaggctgaa tattgcgaat atctgcagaa
    50041 cctgcagtag cataccgaac atgtccaata gccgcattcc cagttaaatt atctaattca
    50101 gattgattct taaaaacttc agaaaggagc ccaacatttc gataaccata gagtttccca
    50161 ttatcatttg aaataatacc agctccttct tgaccgcgat gttgaaggct atgaagccca
    50221 aagtaagtga cttgagctgc ctgaggatac ccccagatac caaagactcc acattcttca
    50281 tttagagatt ttacttcgta tgtcattctt ttttcctaat tctttttgta tctcttctta
    50341 agtagctttg atgattttct caattttgga tctgtcgtca tcaatgccaa cattaaaata
    50401 gaaaatctat caagctagcc attgaacctt agtctcaatg actcccaaat accaatacgg
    50461 cggaaagata cggttaagct cgcttcgttc aaaagatagc gaatttctat ctaacctcgc
    50521 cttcactaat tcaaggcgag ggctaaagct aggacaaaag tcctaagcct ccatattttt
    50581 tataatctag ttcgcaaaac gtaggggttg agtgggctct aattcgctga taaatcaact
    50641 cttacagccc cactcaactg tgcggagggg gacgacaaaa tcgaattcct ttggaattac
    50701 cgatttttgt cccactctca cttccctgta aagtatttta cagcactttc aaagagtttt
    50761 tggtctttgt ttccagggat attttggaag agtccgtctt cccagcgttc tgagtgcccc
    50821 atcttaccga tgatttgacc attcttgctg gtaatccctt cgatggcatt gacagagccg
    50881 tttggattgt atttagaatc catagatggt tgtccgtcaa agtccacata ttggctccag
    50941 atttgaccat tgtctcttag ctctgcaaat tcagaagcgc tgacaacaaa tttaccttca
    51001 ccgtgtgaaa ctggaatggc atgaatatcg ccgacctcaa ctcctgccaa ccaaggtgaa
    51061 ttggtatttg cgatacgagt ctcaaccatc tttgcaacgt gctggttagc atcgttatag
    51121 aagagggttg gacttgtctc accagcttcc tcgaagtttc cgtatggaag aagacctgat
    51181 ttaacaaggg cttggaaacc attacagata ccgatgataa ggccaccttt ttcgatgaag
    51241 ctgtcaatag ctgcgcgaac cttctcgtta agcaagatat tgacgataaa cttagcagag
    51301 ccatctggtt catccgctgc tgagaaacct ccagcaaaga agatgatatt tgccttagca
    51361 atattagcaa ccatcgtatc aactgactca gcaatagcag cctcattcaa ggttacaaat
    51421 ggcaccaagt tgacgctagc tccaacctgt tcaaaggcct tagctgaatc gtactctgag
    51481 ttagtaccag gaaagactgg gatgtaaact actggttttt caataatttc cttagccttg
    51541 ataacaacat ctgataccac agcaggaact tcctcaatag catctacctg ttcaaactct
    51601 gttgggtaaa cttcttctaa tttaccttcg aaggctgata gaaggcttgc accagctagg
    51661 tcatttccat tgacagtgac tgtaaagtct gcctgagttt gaccgatctt cacaacgtca
    51721 gcaatttcct caactgatgt aaagacaaag cctccgagtt gagctgtcaa gctgctgtca
    51781 agctctgcaa tttcaacaga agcaccgata cggttaccaa aagtcatgag agcaagactt
    51841 tctaggacac caccgtattt aacggctgag gcagcggtaa tcttatgttg agcttgaata
    51901 gcctcaaact ggctaaagtt agccttgata aggtcaaaat caatatcttc tgaaatagct
    51961 tgacctggaa tgtagtagat attctcacca gccgctttaa actcaggaga gagaaccttg
    52021 cggctgtccg ctgttgtcac accgaaagct accaaggttg gtggtactgt caattcttcg
    52081 aaagtaccag acatagagtc cttaccgccg attgatggca aaccaaattg aatctgagcc
    52141 tcaatagaac caagaagagc tgatactggc tgaccaaaac gctctgcctg tttatccata
    52201 cgctcgaaat actcttggta agagaaacgt gcacgagacc agtcagcacc cgttgctacc
    52261 aagcgagctg tcgcttcaat aacagcatag gcagcaccgt gataaggtga ccactctgca
    52321 atataaggat tgtaaccttg agccataaca gatgctgttg ttgtcacacc atattgaact
    52381 ggcaattttt gaacagaact ttctgtcggt gtgatttggt aacgaccacc gattgggtgg
    52441 ttaacggttg aacgaccaac agatgagtca aagatagttt gaagaccttt ttgactagcg
    52501 tgattgaggt cagacaagac cttaagcata tctgcttcaa gtgtctctgc tgatgttgtg
    52561 cgcgcttctg gaactgttaa gtccttgtca acgactttgg catcaactac gacacgaaca
    52621 ccgttggtat caaggaaaca gcgttccaaa tcaacaattg tttcaccatt ccaagtcatg
    52681 acaagatttg gtttttcagt aactgtcgca acaacaactg catcgatatt ttccttgtta
    52741 caggctgcga tgaaggcatc cacatcactt ggaccaacaa caactgacat cctttcttga
    52801 gattctgaga tagcaatttc agtaccatta agaccttggt atttaagtgg caccttgtcc
    52861 aaatcgattt caagaccatc agctaattca ccgatggcaa cacagacacc acctgcaccg
    52921 aagtcatttg atttcttgat taggcgagtg acattaccat cacggaaaag gcgttgaatc
    52981 ttacgttctt cgatggcatt ccctttttgt acctctgcgc cagctgtttc cacagattca
    53041 accgtttgaa ccttagatga acctgtcgca ccgccgacac cgtcacgacc tgttttacca
    53101 ccgagcaaga caaccacatc gcctgcctct ggtttttcac gaaccacatt ttccttaggt
    53161 gcagcaccaa ccacagctcc aagctccatg cgtttagcta cgaagccagg gtggaagtac
    53221 tcacgcacat aagttgtcgc aagcccaatt tggttaccat atgaagaata gccgtgcgcc
    53281 gcagtcttag aaataacctg ttgtggcaat ttaccagcac gtgtttccgc aatcggagtc
    53341 gtgatatctc ctgcgcctga aatacgcata gcctgataaa cgtatgaacg tcctgacaat
    53401 gggtcacgaa tggcaccacc gatacaagtc gccgctccac caaatggctc aatttctgtt
    53461 gggtggttgt gagtctcgtt cttaaacatg aggagccaag gctctttcac accatcaaca
    53521 tctacttcaa tttcaactga gcaggcattg atttcatctg agacttccat atcgtccaga
    53581 cgaccattgg cacgctcata acgaccaaag atagtcgcca tatccataag tgtctgcggc
    53641 ttttcagaac gaccaagctc atcacgcatg gcaatgtatt tgtcataagt cgcctgcaat
    53701 tgtttttgga atttagaggc cgaaaaatca atgtttttca attcagtttc aaaggttgtg
    53761 tgacggcagt ggtctgacca atacgtatcc aaaactttca actcagtctc agttggcaca
    53821 cgcccgattg atttgaaata atcttggatg aagagaaggt catcgacctc catagccaat
    53881 ccctgctctg ccttataggc tgcaaaatcg tcagcctgat aagtttcgaa gaaatcaaga
    53941 ttagagattg tcttatcaga cacagagaaa gcctgcactt caagtggcaa ggtaatatcc
    54001 ttgaaacgtg aatcaacggg attcaaaaga tagttcttaa cggcttcaag ctctgcttct
    54061 gcaatatcct tattgaccaa gtaaagctga gctgtattga ccttgacctg actgtcactt
    54121 ccaagcaata gcaaagcttc ttgcgaacta gcagcacgtt ggtcaaattg accaggaagc
    54181 gcctcaatgg caaagaaggc aaccttatca agctccgcag tgatttcagc ttctgtcaaa
    54241 agacggtctg tcacctgctc agagaaaata tgcttctcag cacgcgccag caaatcctca
    54301 gccaaattga agacatcata gacctgcacg atacgcaaat ccttcaaaga agccagttgt
    54361 agattatgcg tcaactcttt cacaagacta gccgatttaa tgccaaagtc agcctttttc
    54421 tcaacaaaaa tacgtttatt catataagat accaagtccg acaagggagc gactcgaaaa
    54481 taggaggacg tctgaaattg agatttcagt aagcccaatc ttttttctga gtttcccgaa
    54541 cggattcaat tccttcctct tctaattaca atgctatcaa cttctcaagt acaacttgat
    54601 agacatcggt caagctacca agatccctac gaaaaacatc cttgtccata tgattaccat
    54661 cagcatccca gagacggcag ttgtcaggtg agaactcatc agccaagatg attttgccat
    54721 ccttgtcaaa tccgaactcc aacttgaagt caatcaagtt aagcccaatc tgagcaaacc
    54781 aatctttcaa gagttcatta atatggcgag tctctccttt cagataagca atctcctcat
    54841 cattaacaat acccaaaaac ttcacatgct catcattgat aaaaggatca ttcagattat
    54901 catttttgta gtaaaattcc acgattggcg tttcaagaac gtgtccttct tccactccaa
    54961 aacgttttga aaatgagcct gctgtcacat tccgtagcac cacctcaagt ggaataatat
    55021 caactttttt attcaattgt tcgttttttg aaatttgctc aatataatgt gtcaccacac
    55081 cagccatatt taatttctca aaaataagag atgaaatctg attatttagt gctcccttgc
    55141 catcaatagt ctccttacga gctccattca acatagtagc ttggtcttta tagaccgttc
    55201 taatcacatt ttcatctttt gtactataaa tatctttcgc ttttcctgta taaataagct
    55261 ggttagtcac ttgattattc gcctttctct tattttaaga gttattctaa cacacaacat
    55321 tatctaatac aacaaaaact ttatttaaaa tcaaaaaaaa gaagaaacgt tcgtattttc
    55381 ttccttttta ttattactta tgttcaatga tataatctaa tagttgttgg acgcttgaca
    55441 tgtgttcgac agcctcatct ggaatctcta gatgaaacac atcttccaaa ttgataataa
    55501 actctgttaa agcgatagag tcaattgcta aatcatcatg taacgatgac tctggtgtga
    55561 tatctaactg tgggtcacca agttgctgcc ttaataattc cagcatcttt tcaaaaactt
    55621 catctcttga catgagttca ctcctattca tctgtaaagg catcaactaa ttgatcaact
    55681 acttgagttt ccaacattgt acgaacttgc ttcaaagtag aataaacagc tttagaatca
    55741 cttgatccat ggcattttac tattggagct tttaaaccaa aaagaaccgc tccacccgcg
    55801 cttgaatagt ccatactatc ttttagctga tataggctat cttttaatag aagtgctcct
    55861 aatttagctt tcacgccacc tgatttaatt gaagatttta ggcttcccat aatactcata
    55921 gctgtacctt ccattgtctt tagaacagca tttccagtaa aaccatcagt gacaacaaca
    55981 tctgctacac tagacatcaa atcacgtgct tcaatatttc caataaaatt aatagaaggc
    56041 tctgctgcta acagttcata agcttcttta tgaagagaat cgccttttgt ttcttccgtt
    56101 ccgttattta agagacctac acgaggaact tcaataccac ggacattttt tgcataaaac
    56161 gaccctaaaa tagcatattg atgaagatga gaagctgtgt tttctgcatt agctcctaaa
    56221 tcaagcatat caaaacccac accatctaac gtaggcatag ttgacattaa gcctggtcga
    56281 tcaacacctt ttatacgtcc tactacaaac aaaccagcag ctaataaagc tcctgtattc
    56341 cctgcagaaa taaaggcctg tgcaacacca tctttgactg ccttagctcc taagaccatc
    56401 gaagactgtt ttttacgacg aacagcttta gcaggctcat catcagaatt aattttttct
    56461 tcagtatgaa taatactaac tcgctccgtt actgttaaat atttctcaat ttttttctga
    56521 tcgccataga gttgtacttc aatatcacta aaatctgaaa tagcttgatt aacaccctct
    56581 acaatagctt tgggagcgta atctccaccc atagcatcta tagcaatttt cttcatgaat
    56641 gaactcctca taaaaactta gtggctatat tatagcacgt ttctttattt ttttcacaag
    56701 aaggcaataa tagctaatat ctaacagata ctctcacgct ttccaaagaa aagtatgcta
    56761 aacaacctac taatatcaaa gaacatagta ctcccccttc aaaaccaaaa tccccaccat
    56821 tccaaagagc aaagctagaa ttttcagtaa ttctaaaaag agaaacagga gaatcccccc
    56881 cactaacctt attacctaat acacatccca tagtaaaatt ccatgtagta tgaactcctg
    56941 taacaaaaag aatatttttg gtcatattaa aaagaataga aaaaataata ccaagtaaaa
    57001 ataaattaaa aagtgctatt gctgtgatac catagtttct aaagtggata aaagaaaaca
    57061 acatagaatt acataaaaca cctgcaaaac tacctttaaa tttggtaact gttgtcataa
    57121 catatcctct aaataaaatt tcctctgtca agctttggat aaagtatgca agaaataaaa
    57181 agaaaaaagg tccaaaagaa aaggaattgc tggaagaaat ttccacaaaa cctgataacc
    57241 agtggcaaat ataaataaaa aacatcagta aactaccagt aataaatcct gagatcgttg
    57301 ttaaaacatt attatcttta gtaaatccca aggaagtaaa atcccttttt tgaaaataga
    57361 ttaaaaatag taaaatactt gccaaaatag gaacaaatag taaaagcgca atgtataaat
    57421 tgaatttata atcatcaggt agcaatttta ctacaaggcg tctacttaca atgatgccga
    57481 taacttgaat tagaactgca ccaataactg gcacaaacca gttag
//
LOCUS       DATRTN010000005        57517 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.005, whole genome shotgun sequence.
ACCESSION   DATRTN010000005 DATRTN010000000
VERSION     DATRTN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 57517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57517
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.005"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..673
                     /locus_tag="VBE22_000302"
     CDS             <1..673
                     /locus_tag="VBE22_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7201781.1"
                     /translation="NATLLTNTFLMPLLYLAMFIVPILNNGKEIGRFFNENYFGIAFL
                     AGILIGSLCVMPASIVGVGISLEKSNFYFIKSLPISFSYFLKHKFVTLITLQLAVPTF
                     IYFLVGFFLLKLSILVLLSFILGLVFMGLIEGQFIYRRDYKHLFLNWQEVTQLFNRGV
                     GQWLLVGSLFGMMIIGSFLIGISIFWSMVWNTVAVNIIILITGLLILSICQYLLLKNF
                     WKKLV"
     gene            complement(881..2266)
                     /locus_tag="VBE22_000303"
     CDS             complement(881..2266)
                     /locus_tag="VBE22_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO7201782.1"
                     /translation="MSREYIALSKNIIKHLGGQNNINNVYHCQTRLRFSLNDPTKVNL
                     EQLKTLKEVKTVVISGGQHQIVIGTHVAKVFEEINSLIETNSTTKTEQTKKAKAVSRI
                     IDFVSGTFQPILPALSGAGMIKALLALLLVFKILTPLSQTYILLNLFADGVFYFLPIL
                     IAITAAQKLKANPILALGTVVMLLHPNWANLVASGKPVSLFHTIPFTLTNYASSVIPI
                     ILIICVQAYIEKYLNQIIPKSLRLVLVPMLIFLSMGILSFSILGPMGTIAGQYLAVIF
                     TFLSKYASWAPAFLVGAFAPILIMFGVHSGIAALGITQLTKLGFDSIFGPGMLCSNIA
                     QATAGTVVTLITKEKKLKEIAGPAAITAYMGITEPILYGVNLPKRYPLIASLIGGGLG
                     GLYAGIMNAHRFAVGSSGLPGLFLYISHTSTHLFITMLIAVIITVSTTAILTFILAQY
                     YEKQVSIRQRD"
     gene            2407..3210
                     /gene="proB"
                     /locus_tag="VBE22_000304"
     CDS             2407..3210
                     /gene="proB"
                     /locus_tag="VBE22_000304"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEO7201783.1"
                     /translation="MKRHFETTRRIVIKVGTSSLVQTSGKINLSKIDHLAFVISSLMN
                     RGMEVILVSSGAMGFGLDILKMDKRPQEISQQQAVSSVGQVAMMSLYSQIFSHYQTHV
                     SQILLTRDVVVFPESLQNVTNSFESLLSMGILPIVNENDAVSVDEMDHKTKFGDNDRL
                     SAVVAKITKADLLIMLSDIDGLFDKNPNIYDDAVLRSHVSEITDDIIKSAGGAGSKFG
                     TGGMLSKIKSAQMVFDNNGQMILMNGANPRDILKVLDGHNIGTYFAQGK"
     gene            3220..4473
                     /locus_tag="VBE22_000305"
     CDS             3220..4473
                     /locus_tag="VBE22_000305"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEO7201784.1"
                     /translation="MTYIEILGQNAKKASQSVARLSTASKNEILRDLARNIVADTETI
                     LTENARDVAKAKDNGISEIMVDRLRLNKDRIQAIANGIYQVADLADPIGQVVSGYTNL
                     DGLKILKKRVPLGVIAMIFESRPNVSVDAFSLAFKTGNAIILRGGKDAIFSNTALVNC
                     MRQTLQDTGHNPDIVQLVEDTSHVVAEELMQATDYVDVLIPRGGAKLIQTVKEKSKIP
                     VIETGVGNVHIYIDEFADLDMAAKIVINAKTQRPSVCNAAEGLVVHQAIAKGFLSQLE
                     KMLKESNQSVEFRADEEALQLLENAVAASESDYATEFLDYIMSVKVVDSFEQAISWIN
                     KYSSHHSEAIITNNISRAEIFQDMVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKLH
                     ARGPMGLEALTSTKYYINGTGQVRE"
     gene            4556..5503
                     /gene="rsmH"
                     /locus_tag="VBE22_000306"
     CDS             4556..5503
                     /gene="rsmH"
                     /locus_tag="VBE22_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEO7201785.1"
                     /translation="MTNDFHHITVLLHETVDMLDIKPDGIYVDATLGGAGHSEYLLSQ
                     LGPDGHLYAFDQDQKAIDNAHIRLKKYVDTGQVTFIKDNFRNLSSNLKALGVSEINGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREQSLTAYDVVNTYSYHDLVRIFFKYG
                     EDKFSKQIARKIEQVRAEKPISTTTELAEIIKSSKSAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDLLAVDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLQPKMELVNRKPILPSQEELEANNRAHSAKLRVARRIR"
     gene            5518..5844
                     /gene="ftsL"
                     /locus_tag="VBE22_000307"
     CDS             5518..5844
                     /gene="ftsL"
                     /locus_tag="VBE22_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEO7201786.1"
                     /translation="MTNEKRTEAVTQTLQRHIKTFSRIEKAFYGAIVITAIIMAVGII
                     YLQSNSLQVKQEVNQLNSKINDKQTEFDNAKQEVNELSNRDRITKIAKDAGLTIQNDN
                     IYRKVD"
     gene            5848..8106
                     /locus_tag="VBE22_000308"
     CDS             5848..8106
                     /locus_tag="VBE22_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7201787.1"
                     /translation="MTFFKKLKKIFLDYVIHIRDRRSPQKNRERVGQNLMILTIFLFF
                     IFIINFVIIVGTDSKFGVNLSKEAKKVYQQSMTVQAKRGTIYDRNGNPIAEDATTYSL
                     YAIISKNYTTATGQKLYVQPSQYEKVASILENKLGMKKNLVLKQLNQKKLFQVSFGSS
                     GSGLSYTKMADIKKTMEKSDIKGIGFSTSPGRIYPNGIFASQFIGFTLPQDDGDGKKL
                     VGNTGLEAALNKVLSGTDGKVTYEKDRSGNVLLGTATTERRAVNGKDIYTTLSEPIQT
                     VLETQMDVFAEKTKGKFASATVVNAKTGEILATSQRPTYNPSTLKGYDKKNLGTYNTL
                     LYDNFFEPGSTMKVMTLASAIDSKHFNSTEVYNSAQYKIADAVIRDWDVNEGLSSGSY
                     MTFPQGFAHSSNVGMVTLEQKMGRDKWLNYLSKFKFGYPTRFGMLHESGGLFPSDNEV
                     TIAMSSFGQGIGVTQVQMLRAFTSISNDGVMLQPQFISSIYDPNTGTSRTARKEVVGK
                     PISKEAASKTRDYMVTVGTDPYYGTLYAAGAPVIQVGNQSVAVKSGTAQIAQEGGGGY
                     LQGKNDTINSVVAMVPSENPDFIMYVTIQQPEKFSITFWKDVVNPVLEQATAMKETIL
                     KPGLNDSEHQTKYKLSKIVGENPGHVAEELRRNLVQPIILGNGSKVSKVSKRPGANLA
                     ENEQLLVLTNKLTELPDMYGWSKANVEQFAKWTGIKVTYKGSTSGKVRKQSIDVGKSI
                     NKIKKIKITIGD"
     gene            8108..9118
                     /locus_tag="VBE22_000309"
     CDS             8108..9118
                     /locus_tag="VBE22_000309"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEO7201788.1"
                     /translation="MFLSIMAGVIAFVLTVIAIPRFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLIVALLVSLIFSIILSKENSGNLGATFGILSVVLIYGIIGFLDDF
                     LKIFKQINEGLTPKQKMSLQLIAGLIFYFVHVLPSGTSAINIFGFNLEVGYLYAFFVL
                     FWVVGFSNAVNLTDGIDGLASISVVISLITYGIIAYNQTQFDILLIIVIMIGALLGFF
                     VFNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLFIGFVYVFETSSVMLQVAYFK
                     YTKKKTGVGKRIFRMTPFHHHLELGGVSGKGNKWSEWKVDAFLWAIGIFMSAITLAIL
                     YL"
     gene            9216..10559
                     /locus_tag="VBE22_000310"
     CDS             9216..10559
                     /locus_tag="VBE22_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7201789.1"
                     /translation="MSFKDFNFKPYIQRALDELKFVDPTDVQAKLIPVVRSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDESSNDVQVVITAPSRELATQIYQATKQIAEHSEQEIRV
                     VNYVGGTDKLRQIEKLKVSQPHIVIGTPGRIYDLVKSGDLAIHKAHTFVVDEADMTLD
                     MGFLDTVDKIAGSLPKDVQILVFSATIPQKLQPFLKKYLTNPVMEKIKTATVITDTID
                     NWLLSTKGRDKNAQILELSKLMQPYLAMIFVNTKERADELHSYLSSNGLKVAKIHGGI
                     APRERKRIMNQVKNLEFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGTAITLYQPSDDSDIRELEKLGINFIPKVIKNGEFQDTYDRDRRNNREKSYQK
                     LDTEMIGLVKKKKKKIKPGYKKKIQWKVDEKRRKERRASNRAKGRAERKAKKQSF"
     gene            10697..11509
                     /locus_tag="VBE22_000311"
     CDS             10697..11509
                     /locus_tag="VBE22_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7201790.1"
                     /translation="MFKKIILSIATIVATASLAVSVQASEKVELKVATDSDTAPFTYQ
                     KDGKFKGYDVDVVKAVFKGSKYKVTFKTVPFDTISTGIDAGKFDLSANDFSYNKERAE
                     KYLFSEPISRSNYAVVGKKGSHYKSLSDLSGKSTEVLSGVNYAQVLENWNKNHPNKKP
                     IKIKYVSGTTGVTSRLKNIESGKIDFILYDAISSDYIVKDQSLNLSVSPLKGKIGNNK
                     DGLEYLLLPKDKKGKTLQKFINKRIKVLKEDGTLARLSKQYFGGDYVSNIDK"
     gene            11604..12407
                     /locus_tag="VBE22_000312"
     CDS             11604..12407
                     /locus_tag="VBE22_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7201791.1"
                     /translation="MNQFILTGGWSWYNNLVSQVPAGKLFSWKAVLDAIPSILERLPI
                     TLLLTVAGALFGLILALIFAIVKINRVKILYPIQALFVSFLRGTPILVQLMLSYYGIP
                     LFLKFLNQKYGFDWNINAIPASVFAITAFAFNEAAYTSETIRAAILSVDQGEIEAARS
                     LGMTSAQVYRRVIIPNAAVVATPTLINTLIGLTKGTSLAFNAGIVEMFAQAQIMGGSD
                     YRYFERYISVALVYWAVSFLIEQLGNAIERKMAIKAPRHLTDEIPGGVR"
     gene            12407..13150
                     /locus_tag="VBE22_000313"
     CDS             12407..13150
                     /locus_tag="VBE22_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7201792.1"
                     /translation="MIKLRQLTKSFSGQKVLDKLDLDIEKGQVVALVGASGAGKSTFL
                     RSMNYLEEPDYGTIEIDDFKVDFKSISKDDILTLRRKLAMVFQQFNLFERRTALDNVK
                     EGLKIVKKMSDQEATRIARDELAKVGLADREKYYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKQGQTMVLVSHDMNFVYQVADKVLFLEKGRIL
                     ESGTPEQLFNHPLEERTKEFFASYNKSYL"
     gene            13272..13496
                     /locus_tag="VBE22_000314"
     CDS             13272..13496
                     /locus_tag="VBE22_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEO7201793.1"
                     /translation="MFLEIAKVYLQGLGLASITVILVGIIWVFWRAKKNIDKTARERQ
                     SVLYDVLLIALMTAPILAFAFVAIILMIKA"
     gene            13565..14479
                     /gene="trxB"
                     /locus_tag="VBE22_000315"
     CDS             13565..14479
                     /gene="trxB"
                     /locus_tag="VBE22_000315"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEO7201794.1"
                     /translation="MYDTLIIGSGPGGMTAALYAARSNLKVGLIEQGAPGGQMNNTAE
                     IENYPGYDHISGPELSMKMYEPLEKFEVEHIYGIVQRVENDGDVKRVITEDESYEAKT
                     VILATGAKNSLLGVPGEEEYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAVFLT
                     QFAKSVTIIHRRDQLRAQKVLQDRAFANEKIKFVWDSVVKEIKGNEIKVSGVTVENLK
                     TGEISEMPFGGVFIYVGLKPHSSMVSELGITDETGWVLTDTNMKTSIPGLYAIGDVRQ
                     KDLRQIATAVGEGAIAGQGVYNYITEHF"
     gene            14635..16095
                     /locus_tag="VBE22_000316"
     CDS             14635..16095
                     /locus_tag="VBE22_000316"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEO7201795.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFNKGIHNKKAVFEAYFRKVPFE
                     NGYAVFAGLERIVRYLENLSFSDSDLSYLEELGYPEEFLDYLKNLKMELTVKSAKEGD
                     LVFANEPLVQIEGPLAQCQLVETAILNIINYQTLVATKAARIRSVIEDEPLLEFGTRR
                     AQEMDAAIWGTRAAIIGGANATSNVRAGKIFNIPVSGTHAHALVQTYGDDYQAFKAYA
                     ETHKDCVFLVDTYDTLRVGVPNAIRVAKEMGEKINFLGVRLDSGDLAYLSKKVRQQLD
                     DAGFPNAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     TDAGSMRDTIKLSNNAEKVSTPGKKQVWRITSRAKGKSEGDYITFADTDVTQLDEIDM
                     FHPTYTYINKTVRDFDAVPLLVDIFDKGKLVYQLPSLQEIQEYGRKEFDQLWDEYKRV
                     LNPQDYPVDLARDVWQNKMDLIDRIRKEALAKGEVR"
     gene            16092..16913
                     /gene="nadE"
                     /locus_tag="VBE22_000317"
     CDS             16092..16913
                     /gene="nadE"
                     /locus_tag="VBE22_000317"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEO7201796.1"
                     /translation="MTLQDQIIKELGVKPVINPSQEIRRSVEFLKDYLLKHSFLKTYV
                     LGISGGQDSTLAGRLAQLAVEELRADTGENYQFIAIRLPYGIQADEEDAQKALDFIKP
                     DIALTINIKEAVDGQVRALNAAGVEITDFNKGNIKARQRMISQYAVAGQYAGAVIGTD
                     HAAENITGFFTKFGDGGADLLPLFRLNKSQGKQLLAELGADKALYEKIPTADLEENKP
                     GIADEIALGVTYQEIDAYLEGKVVSDKSRDIIENWWYKGQHKRHLPITIFDDFWK"
     gene            17026..18360
                     /gene="pepC"
                     /locus_tag="VBE22_000318"
     CDS             17026..18360
                     /gene="pepC"
                     /locus_tag="VBE22_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEO7201797.1"
                     /translation="MSELTQTFTDKLFADYQANTKFSAIENAVTHNGLLKSLETRQSE
                     IENDYVFSIDLTKDEVSNQKQSGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFMEQIIATANQELSSRKVKFLLDVPQQDGGQWDMVVALFEKYGVVPKTVYP
                     ESVSSSASRELNQYLNKLLRQDAQILRELIAQGADGATVQNKKEELLQEIFNFLAMNL
                     GLPPQSFDFAYRDKDNHYQSDKNITPKAFYQKYVNLDLSDYVSIINAPTVDKPYGQSY
                     TVEMLGNVVGGPAVKYLNLDMKRFKELAIAQMKSGETVWFGSDVGQVSNRQKGILATT
                     TYDFNSSMDIKLSQDKAGRLDYSESLMTHAMVLTGVDLDESGQPLKWKVENSWGEKVG
                     KDGYFVASDAWMDEYTYQIVVRKELLTKEELEAYNAEPITLAPWDPMGALAN"
     gene            complement(18407..20647)
                     /locus_tag="VBE22_000319"
     CDS             complement(18407..20647)
                     /locus_tag="VBE22_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7201798.1"
                     /translation="MITIKKESVIKLLKYAFGIIMGFIILTIVIGGLLFAYYVSRSPK
                     LTDQALKSVNSSLVYDGNNKLIADLGSEKRESVSADSIPLNLVNAITSIEDKRFFKHR
                     GVDIYRILGAAWHNLVSSNTQGGSTLDQQLIKLAYFSTNKSDQTLKRKSQEVWLALQM
                     ERKYTKEEILTFYINKVYMGNGNYGMRTTAKSYFGKDLKELSIAQLALLAGIPQAPTQ
                     YDPYKNPESAQTRRNTVLQQMYQDKNISKKEYDQAVATPVTDGLKELKQKSTYPKYMD
                     NYLKQVISEVKQKTGKDIFTAGLKVYTNINTDAQKQLYDIYNSDTYIAYPNNELQIAS
                     TIMDATNGKVIAQLGGRHQNENISFGTNQSVLTDRDWGSTMKPISAYAPAIDSGVYNS
                     TGQSLNDSVYYWPGTSTQLYDWDRQYMGWMSMQTAIQQSRNVPAVRALEAAGLDEAKS
                     FLEKLGIYYPEMNYSNAISSNNSSSDAKYGASSEKMAAAYSAFANGGTYYKPQYVNKI
                     EFSDGTNDTYAASGSRAMKETTAYMMTDMLKTVLTFGTGTKAAIPGVAQAGKTGTSNY
                     TEDELAKIEATTGIYNSAVGTMAPDENFVGYTSKYTMAIWTGYKNRLTPLYGSQLDIA
                     TEVYRAMMSYLTGGYSADWTMPEGLYRSGSYLYINGTTTTGTYSSSVYKNIYQNSGQS
                     SQSSSSTSSEKQKEDKNTANDANSSSPQVETPNNGNATTPNNSNQTVPGTGHGNGNSN
                     TAPNGN"
     gene            complement(20634..21233)
                     /gene="recU"
                     /locus_tag="VBE22_000320"
     CDS             complement(20634..21233)
                     /gene="recU"
                     /locus_tag="VBE22_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEO7201799.1"
                     /translation="MVNYPHQLIRKTTVTKSKKKKIDFANRGMSFEAAINATNDYYLS
                     HELAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVYKGYYIDFEAKETR
                     QKTAMPMKNFHAHQIEHMANVLQQKGICFVLLHFSTLKETYLLPAHELISFYQIDKGN
                     KSMPIDYIRKNGFFVKESAFPQVPYLDIIEEKLLGGDYN"
     gene            21308..21826
                     /locus_tag="VBE22_000321"
     CDS             21308..21826
                     /locus_tag="VBE22_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEO7201800.1"
                     /translation="MKSTILVTGYKNFELGIFQDKDPRITIIKKAIDKDFRRFLENGA
                     DWFIFMGNLGFEYWALEVALDLQKEYDFQIATIFTFENHGQNWNEANKAKLALFKQVD
                     FVKYTFPSYENPGQFKQYNHFLINNTQGAYLFYDSENETNLKFLLEMMKKKEAYDISF
                     LTFDRLNEIYEE"
     gene            21947..22279
                     /gene="gpsB"
                     /locus_tag="VBE22_000322"
     CDS             21947..22279
                     /gene="gpsB"
                     /locus_tag="VBE22_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEO7201801.1"
                     /translation="MASIIYSPKDIFEQDFKVSMRGYDKKEVDVFLDDVIKDYENYLE
                     QIEKLQMENRRLQQALDKKEREASNVRNSGTAMYNQKPIAQSATNFDILKRISRLEKE
                     VFGRQIRE"
     gene            22298..22666
                     /gene="rnpB"
                     /locus_tag="VBE22_000323"
     ncRNA           22298..22666
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VBE22_000323"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            22749..23903
                     /locus_tag="VBE22_000324"
     CDS             22749..23903
                     /locus_tag="VBE22_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEO7201802.1"
                     /translation="MKESFKLIATAAAGLEAIVGREIRNLGIDCQVENGRVRFHGDIK
                     TIIETNLWLRAADRIKIIVGEFPARTFEELFQGVYGLDWENYLPLGAKFPIAKAKCVK
                     SKLHNEPSVQAISKKAVAKKLQKVFHRPEGVPLQENGAEFKIEVSILKDKATVMIDTT
                     GSSLFKRGYRAEKGGAPIKENMAAAIIQLSNWFPDKPLIDPTCGSGTFCIEAAMIGMN
                     IAPGFNRDFAFEAWPWVDQSQVQKVRDEAESKANYDIDLDISGFDLDGRMVEIARKNA
                     EEAGLGDVIKLKQMRLQDLKTDKINGVIISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDEGFEKKYGSQADKKRKLYNGTLKVDLYQYYGERVRRQVK"
     gene            23916..25379
                     /locus_tag="VBE22_000325"
     CDS             23916..25379
                     /locus_tag="VBE22_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201803.1"
                     /translation="MSEDQKHPFFEPEKETDGLEFKDAKEMTVEEAVRKDSEIKAGIT
                     EEDSILDKYIKQHRDEVASQKFETKSSDFANLDTASLDDFIKKQREELSAMLAAEELS
                     KKLDNSVSQEQDTEANAVSPKEESSQEQENSVTPVPPLITEAEPTATEPDSTIADSEE
                     YKSSSRKRGGIVGTLIALILLLIVAIFGYNYFKNNNSTNSQTATSQSSSSKATTTSSE
                     EDKKASQNLDNFNKAYANFFVDDKKTQLKNSEFDKLSELEKKVDALKGTKYYGKVKVK
                     FDSLKRQIDAVKAVNDKFKSPAVVDGKKSEKLEVKDGANFDSLDSKTLNTGNASLDSL
                     LHSIVSTGRNQVKQSEEQASSNKVSDTQTTEQPNVTNGQSSSSAATINNQAAGTASGN
                     LERNRSRVPYNNAAIADTGNPAWIFNPGVLEKIVATSQARGYFSGNNYILEPVNIING
                     NGYYNMFKSDGTYLFSINAKTGYFVGNAPGRADSLDY"
     gene            complement(25604..26086)
                     /locus_tag="VBE22_000326"
     CDS             complement(25604..26086)
                     /locus_tag="VBE22_000326"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEO7201804.1"
                     /translation="MTKEVVVESFELDHTIVKAPYVRLISEEVGPVGDIITNFDIRLI
                     QPNENAIDTAGLHTIEHLLAKLIRQRINGLIDCSPFGCRTGFHMIMWGKQDATEIAKV
                     IKSSLEAIAGGVTWEDVPGTTIESCGNYKDHSLHSAQEWAKLILSQGISDNAFERHIV
                     "
     gene            26275..27882
                     /locus_tag="VBE22_000327"
     CDS             26275..27882
                     /locus_tag="VBE22_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEO7201805.1"
                     /translation="MFNIILAMVCALIGLIIGYVAISMKMKSSKEAAELTLLNAEQDA
                     VDLRGKAEIEAEHIRKAAERESKAHQKELLLEAKEEARKYREEIEKEFKSDRQELKQM
                     EARLTDRASSLDRKDENLSNKEKMLDSKEQSLTDKSRHINEREQEIATLETKKVEELS
                     RIAELSQEEAKDIILADTEKDLAHDIATRIKEAEREVKERSNKIAKDLLAQAMQRLAG
                     EYVTEQTITTVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVVLSGFDPI
                     RREIARMTLESLIQDGRIHPARIEELVEKNRLEMDQRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSYGQNVLRHSVEVGKLAGILAGELGENVDLARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPIVVNTIASHHGDVEPDSVIAVIVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIANGFEGVQNAFALQAGREIRIMVQPGKVSDDQVVIMSHKVRE
                     KIEQNLDYPGNIKVTVIREMRAVDFAK"
     gene            28011..28493
                     /locus_tag="VBE22_000328"
     CDS             28011..28493
                     /locus_tag="VBE22_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7201806.1"
                     /translation="MAKDIIKVDHIFKSIGQKTILEDISFSIASNQCVALIGPNGAGK
                     TTLMSTLLGDISISSGALTIFNLPAHHNRLKYKMAILPQENVLPSKFTVRELIDFQRC
                     LFPEVLPMSLILDYLQWSDAHLQQFTETLSGGQKRLLAFVLTLVGKPQLLFLDEPTSG
                     "
     gene            28474..29214
                     /locus_tag="VBE22_000329"
     CDS             28474..29214
                     /locus_tag="VBE22_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEO7201807.1"
                     /translation="MNLLPDKLSDFGDRITFLIVPIFCIGYMWIAIENRNSEEQREKI
                     AEQNQYINILSAENERNRIGRDLHDSLGHTFAMMTLKTELALKLLEKRNYDKVQKELS
                     ELNHISHQSMSEVRQIVSNLKYRTVVEEIDELYRLFQLSNIKLTVVNKLETSQLSPVT
                     QSTITMILKELSNNIVKHAEADSVELSLVRQGATINIEMIDNGCGFTNLDGDELHSIQ
                     ERLTIVEGTLTILSRSKPTHIQVVLKEV"
     gene            29216..29806
                     /locus_tag="VBE22_000330"
     CDS             29216..29806
                     /locus_tag="VBE22_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7201808.1"
                     /translation="MKLLVAEDQSMLRDAMCQLLLMEESVSTIDQAGNGEEAIAILSN
                     KAIDVAILDVEMPILSGLDVLEWVRKYQNVKVIIVTTFKRSGYFQRAIRSNVDAYVLK
                     DRSVADLMKTIQKVLSGGKEYSPELMENVISNPLSEQEIKILSLIAQGKTSKEIACTL
                     FLSNGTVRNYTSNIFDKLGVNNRLEALKIAREKDWL"
     gene            29856..30560
                     /locus_tag="VBE22_000331"
     CDS             29856..30560
                     /locus_tag="VBE22_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEO7201809.1"
                     /translation="MQKFKERFDTLSDAILAIAMTILVLEIKTPATMGDIGDFTRNIG
                     LFIVSFVVVFNFWYERAQNSLDAQKTNDEIIALDIIEHLGICLIPLFTKFMISFENHN
                     FAVMAYGLLTLLVGLTSDIIRIHLASYDLVTIPSELKERVIKVMTTFAIRSVVVRFII
                     IILAYFLPEVGIFAYLVIPLFMFWRRSRAGKYYAKKGIEAPSYIKLFIQIGERVPEDF
                     TNSSSGIGGVKRLYKK"
     gene            30727..31356
                     /gene="gmk"
                     /locus_tag="VBE22_000332"
     CDS             30727..31356
                     /gene="gmk"
                     /locus_tag="VBE22_000332"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEO7201810.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFDYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEALIKEGQMLEYAEYVGNYYGTPLSYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFIFLTPPDLEELEERLVGRGTDSPEVIAQRIERAKEEIALM
                     REYDYAVVNDQVSLAAERVKRVIEAEHYRVDRVIGRYTNMVKETDKKLS"
     gene            31379..31693
                     /locus_tag="VBE22_000333"
     CDS             31379..31693
                     /locus_tag="VBE22_000333"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO7201811.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGEKATQDFKSV
                     KSTLRALEEIESGNVVIHPDPSAKRASVRARIEAERLAKEEEERKIKEQIAKEKEDGE
                     KI"
     gene            31767..34157
                     /locus_tag="VBE22_000334"
     CDS             31767..34157
                     /locus_tag="VBE22_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEO7201812.1"
                     /translation="MARKLAQVIVDIPLMQTDKPFSYAIPKDLEDLVQVGARVHVPFG
                     RGNRLLQGFVVGFRDDDELETKDIAGVLDFEPVLNQEQLDLADQMRHTVFSYKISILK
                     SMLPSLLNSQYDKLLLATDTLPSEDREDLFGHKTEIVFSSLSSQDAKKAGRLIQKGFI
                     EVQYLAKDKKTIKTEKIYKINRTLLEKSQIAARAKKRLELKEFLLENPQPGRLTALNK
                     QFSSPVVNFFREEGIIEVIEKEASRSDNYFKGILKTDFLDLNQEQAKVVKIVVDRIGK
                     DQNKPFLLEGITGSGKTEVYLHIIDNVLKLGKTAIVLVPEISLTPQMTNRFISRFGKR
                     VAIMHSGLSDGEKFDEWRKIKSGQAKVVVGARSAIFAPLENIGAIIIDEEHESTYKQE
                     SNPRYHARDVALLRAEYHKAVLLMGSATPSIESRARASRDVYKFLELKHRANPKARIP
                     QVEIIDFRNFIGQQEVSNFTPYLLDKIRDRLDKKEQVVLMLNRRGYSSFIMCRDCGYV
                     DQCPNCDISLTLHMATKTMNCHYCGFEKPIPRTCPNCNSKSISYYGTGTQKAYEELLK
                     VIPDAKILRMDVDTTRQKGGHESILKRFGNHEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTSLNLPDFRSSERTFQLLTQVAGRAGRAEKEGEVVIQTYNPNHYAIQLAQKQDFEA
                     FYQYEMNIRRQLGYPPYYFTVGLTLSHKDEEWLIRKSYEVLSLLKQGFSDKVKLLGPT
                     PKPIARTHNLYHYQIIIKYRFEDNLELVLNRLLDMTQDKENRDLRLAIDHEPQNMM"
     gene            34204..35139
                     /locus_tag="VBE22_000335"
     CDS             34204..35139
                     /locus_tag="VBE22_000335"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEO7201813.1"
                     /translation="MTKLLFMGTPDFSATVLKGILADGKYDVLAVVTQPDRAVGRKKE
                     IKMTPVKEVALENNIPVYQPEKLSGSPELEQLMTLGADGIVTAAFGQFLPTKLLESVG
                     FAINVHASLLPKYRGGAPIHYAIINGEKEAGVTIMEMVAKMDAGDMVSKASVEITDED
                     NVGTMFDRLAVVGRDLLLDTLPGYLSGDIKPIPQNEEEVSFSPNISPDEERIDWNKSS
                     RDIFNHVRGMYPWPVAHTLLEGNRFKLYEVTMSEGKGSPGQVIAKTKNSLTVATGDGA
                     IELKSVQPAGKPRMDIKDFLNGVGRNLEIGDKFGE"
     gene            35129..36451
                     /gene="rsmB"
                     /locus_tag="VBE22_000336"
     CDS             35129..36451
                     /gene="rsmB"
                     /locus_tag="VBE22_000336"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEO7201814.1"
                     /translation="MANDWKKSARGLALMTLEEVFDKGAYSNIALNKSLKKSRLSDKD
                     RALVTEIVYGTVARKITLEWYLSHFIVDRDKLELWVYHLLLLSLYQLLYLDNIPDHAI
                     VNDAVTIAKNRGNKKGAEKLINAVLRRVSSETLPEIASIKRQNKRYSVAYSMPVWLVK
                     KLIDQYGETRALAIMESLFERNKASLRVTDLSQKQTIKETLNVRDSHIAETALVADSG
                     NFASTSFFQDGLITIQDESSQLVAPTLKVSGNDQVLDACSAPGGKTSHIASYLTTGAV
                     TALDLYDHKLELVMENAKRLGLSDKIKTKKLDASKAHEYFPEDTFDKILVDAPCSGIG
                     LIRRKPDIKYNKANQDFEALQEIQLSILSSVCQTLRKGGIITYSTCTIFEEENFQVIE
                     KFLANHPNFEQVELSHTQEDIVKRGCISISPEQYHTDGFFIGQVKRIL"
     gene            36489..37226
                     /locus_tag="VBE22_000337"
     CDS             36489..37226
                     /locus_tag="VBE22_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEO7201815.1"
                     /translation="MEISLLTDIGQRRSNNQDFINQFENKAGVPLIILADGMGGHRAG
                     NIASEMTVTDLGSDWAETDFSELSEIRDWMLVSIETENRKIYELGQSDDYKGMGTTIE
                     AVAIVGDNIIFAHVGDSRIGIVRQGEYHLLTSDHSLVNELVKAGQLTEEEAASHPQKN
                     IITQSIGQANPVEPDLGVHLLEEGDYLVVNSDGLTNMLSNADIATVLTQEKNLDDKNQ
                     DLITLANHRGGLDNITVALVYVESEAV"
     gene            37226..39181
                     /gene="pknB"
                     /locus_tag="VBE22_000338"
     CDS             37226..39181
                     /gene="pknB"
                     /locus_tag="VBE22_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEO7201816.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLARDLILDNEEVAIKVL
                     RTNYQTDQIAVARFQREARAMAELTHPNIVAIRDIGEEDGQQFLVMEYVDGFDLKKYI
                     QDNAPLSNNEVVRIMNEVLSAMSLAHQKGIVHRDLKPQNILLTKKGTVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSILAENKSVPQALENIVIKATAKKLTDRYKTTYEMGRDLSTALSST
                     RHREPKLVFNDTESTKTLPKVTSTVSSLTTEQLLRNQKQAKTTEKITPDSASNDKTKS
                     KKKASHRLLGTIMKLFFALCVVGIIVFAYKILVSPTTIRVPDVSNKTVAQAKMTLENS
                     GLKVGAIRNIESDSVSEGLVVKTDPAAGRSRREGAKVNLYIATPNKSFTLGNYKEHNY
                     KDILKDLQGKGVKKSLIKVRRKINNDYTTGTILAQSLPEGTSFNPDGNKKLTLTVAVN
                     DPMIMPDVTGMTVGEVIETLTDLGLDADNLVFYQMQNGVYQAVVTPPSSSKIASQDPY
                     YGGEVGLRRGDKVKLYLLGSKTTNNSSSTPIDSSASSSTGTTTSDSVSSSTDASTSDS
                     SSTSTSSSTLPSDSTTNTGTANNPLTQ"
     gene            39340..40035
                     /locus_tag="VBE22_000339"
     CDS             39340..40035
                     /locus_tag="VBE22_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO7201817.1"
                     /translation="MKKFQFFLLVEAVVLVMGLMKILSDDWTSFIFILALILLALRFY
                     NNDSRHNFLLTTSLLLLFLIFMLNPYIIAAVVFAVLYVLINHFSQVKKKNRYALIQFK
                     NHQLDVKTTRNQWLGTDQHESDFYAFEDINIIRISGTDTIDLTNVIVSGQDNVIIIQK
                     VFGDTKVLVPLDVAVKADISSVYGSVQYFDFEEYDLRNESIKLSQEEEYYLLKRVKLV
                     VNTIAGKVEVSRK"
     gene            40032..41051
                     /locus_tag="VBE22_000340"
     CDS             40032..41051
                     /locus_tag="VBE22_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7201818.1"
                     /translation="MKKHHYFLAFFYGSVIIFAICFVIIDSLGVNLVHLYQTSRLWLI
                     EQLIFSIFFLSLAVTILLLLTWFLLDDNSKRQINHNLRRILNNQSINVTDDGTEISTN
                     IQRLSKKMNLMTASLQSKENSRILKSQEIVKQERKRIARDLHDTVSQDLFAASMVLSG
                     IAQNVSQLDVDQVGSQLLAVEEMLQHAQNDLRILLLHLRPVELENKTLSEGFRMILKE
                     LTDKSDIEVVYHESILTLPKKIEDNIFRIGQEFISNTLKHSQASRLEVYLNQTENELQ
                     LKMIDNGIGFDMDSVYDLSYGLKNIEDRVEDLAGNLQLLSQPGKGVAMDIRLPLVNQS
                     EDKNG"
     gene            41044..41685
                     /locus_tag="VBE22_000341"
     CDS             41044..41685
                     /locus_tag="VBE22_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7201819.1"
                     /translation="MDKIKIVLVDDHEMVRLGLKSFLNLQADVEVIGEASNGLEGIKK
                     ALELRPDVVVMDLVMPEMDGVEATLALLKDWPEAAILVLTSYLDNEKIYPVIEAGAKG
                     YMLKTSSAAEILNAIRKVARGEQAIENEVDKKIKAHDKCPALHEGLTARERDILNLLA
                     KGYDNQRIADELFISLKTVKTHVSNILGKLNVADRTQAVVYAFQHHLVPQDDE"
     gene            41727..43127
                     /locus_tag="VBE22_000342"
     CDS             41727..43127
                     /locus_tag="VBE22_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEO7201820.1"
                     /translation="MDAKTKYKAKKIKAVFFDIDDTLRVKDTGYMPPSILKVFKALKD
                     KGIVVGIASGRARYGVPKEVQDLNADYCVKLNGAYVKDKDKNIIFHRPIPAEYVEQYK
                     KWADTVGIKYGLAGRHEAVLSDRDDLVNDAIDIVYSDLEVNPDFNKEHDIYQMWTFED
                     KGDSLHLPEPLDEHLRLIRWHDHSSDVVLKGTSKALGVSKVVEHLGLKPENILVFGDE
                     LNDLELFDYAGLAVAMGVSHPEAQKKADFITKKVEEDGILYALEELGLIEKELTFPQV
                     DIENTEGPVAVIKTNHGDMTVKLFPDHAPKTVANFIGLAKQGYYDGIIFHRIIPDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNVRGALSMANAGPNTNGSQFFIVQNTKIP
                     YAKKELERGGWPTPIAELYAGQGGTPHLDRRHSVFGQLVNQSSFEVLDEIAAVETGSQ
                     DKPLEDVVILTIEVKE"
     gene            43129..43485
                     /locus_tag="VBE22_000343"
     CDS             43129..43485
                     /locus_tag="VBE22_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEO7201821.1"
                     /translation="MKIGDKLKGTVTGIRPYGAFVSLEDGRTGLIHISEIKTGYIDNI
                     YDVLSVGDEVYVQVIDVDEFTQKASLSLRTLEEERHHIQHRHRFSNNRLKIGFKPLEE
                     NLPSWVEEGLAYLNNK"
     gene            complement(43532..44308)
                     /locus_tag="VBE22_000344"
     CDS             complement(43532..44308)
                     /locus_tag="VBE22_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEO7201822.1"
                     /translation="MTNEKGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KMVPETMRDAITNESVIVGEEKSVDDIIEEVLKDIDFYEESGGGITLSGGEIFAQFEF
                     AKAILKRAKSLGIHTAIETTAYTRHEQFIDLIQYVDFIYTDLKHYNSLKHQEKTMVKN
                     ASIIKNIHYAFANGKTIVLRIPVIPNFNDSLEDAEEFACLFDRLDIRQVQLLPFHQFG
                     QNKYQLLNRQYEMEEIAALHPEDLLDYQAIFSKYNIHCYF"
     gene            44429..45184
                     /locus_tag="VBE22_000345"
     CDS             44429..45184
                     /locus_tag="VBE22_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO7201823.1"
                     /translation="MNRLENIISLVSQYQKIDVNTLSELLQVSKVTIRKDLDKLEGKG
                     LLHREHGYAVLNSGDDLNVRLSFNHKTKKEIAALAANMVSDNDTILIESGSTCALLAE
                     NICQTKRNVTILTNSCFIANYLREYDSCQIVLLGGEYQSSSQVTVGPLLKKMISLFHV
                     SLAFVGTDGFDPKLGFMGRDLMRSEVAQEMANAADEVIILTDSSKFNQTALVEQLPLS
                     TVSQVITDKHPNSEIANLFQEAEITIQSLLQVP"
     gene            45303..46286
                     /locus_tag="VBE22_000346"
     CDS             45303..46286
                     /locus_tag="VBE22_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEO7201824.1"
                     /translation="MKLERQKLLAKVAYLYYMEGKSQSEIANELGIYRTTISRMLAKA
                     REEGLVRIEISDFNPEIFQLESYFKSKYHLKDIEIVSSRKDSDTSEIEKDLAHVAAAM
                     IRKKIKENDKVGIAWGRTLSKVVEAMRPHPVSQVSFVPLAGGPSHINARYHVNTLVYE
                     MSRRFQGSCTFINATLVQENANLAKGILTSKYFEGLMDNWEKLDVAIVGVGGKPKSNE
                     QQWLDLLNQDDFQCLDEEAAVGEITCRFFNHSGDPVNQHLAKRTIGITLEQLQKVPNR
                     IAVAHGNYKAAALLAVLKKGYINHLVTDFSTALNILRLDKDTFVDTIYQKS"
     gene            46490..46813
                     /locus_tag="VBE22_000347"
     CDS             46490..46813
                     /locus_tag="VBE22_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO7201825.1"
                     /translation="MEIIVADQIIMGLILNAGDAKQHIYQALKLAKEGNFAESKIEIE
                     LADSALLEAHNLQTQFLAQEAGGTRTDISALFIHSQDHLMTSITEINLIKEIIDLRQE
                     LHIQK"
     gene            46830..47150
                     /locus_tag="VBE22_000348"
     CDS             46830..47150
                     /locus_tag="VBE22_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7201826.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKIAADKEGIEAHIEAYSQGKIAD
                     YAKDLDVALLGPQVSYTLDKSKSICDEYGVPIAVIPMADYGMLDGVKVLKLALSLLET
                     RKEA"
     gene            47152..48453
                     /locus_tag="VBE22_000349"
     CDS             47152..48453
                     /locus_tag="VBE22_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7201827.1"
                     /translation="MSKFDSQKIITPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     LGQLPFKGLNQAIANVFGPEWTEPFMQVYSGTFAIMGLISCFAIAYAYAKNSSVEPLP
                     AGVLSLSSFFILMKSSYIPVKGEAIADAISKVWFGGQGIIGAIIIGLVVGAIYTWFIQ
                     HHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYLIAKVTTGGTFIEMIYDIIQVP
                     LQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKSLLAANRLTL
                     DNGAHIVTQQFLDSFLILSGSGITFGLVIAMLFAAKSKQYKALGKVAAFPAIFNVNEP
                     IVFGFPIVMNPVMFLPFILVPVLAALIVYGAIAVGFMQPFSGVTLPWSTPAIISGFMV
                     GGWQGALVQIVILAISTAVYFPFFKIQDNITYKNECEMERV"
     gene            48634..51090
                     /locus_tag="VBE22_000350"
     CDS             48634..51090
                     /locus_tag="VBE22_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEO7201828.1"
                     /translation="MTKQLLTQKYTNDVQQNSQKHFGYLTERMYSYRDKVLDKKPFID
                     AERAILVTEAYQKHQEKPNVLKRAYMLQNILEKMTIYIDDETMIVGNQASSDKDAPIF
                     PEYTLEFVVNELDLFEKRDGDVFYITEETKEQIRNIAPFWENNNLRARAGVMLPEEVQ
                     VYMETGFFGMEGKMNSGDAHLAVNYQKLLEEGLIGFEKKARKAKADLDLTKPESIDKY
                     HFYDSILITIEAVKTYAERFAILAKKQAKTANAKRRQELLDIASICERVPYYPAKTFA
                     EAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDSIVERLTNLWI
                     KTITINKVRSQAHTFSSAGSPLYQNVTIGGQTRHKEDAVNPLSFLVLKSVAQTHLPQP
                     NLTVRYHANLDKSFMNEAIEVMKLGFGMPAFNNDEIIIPSFIKKGVSEEDAYDYSAIG
                     CVETAVPGKWGYRCTGMSYINFPKVLLITMNNGIDPASGKRFAPSYGHFTQMTSYKEL
                     KEAWDKTLRYLTRMSVIVENAIDISLEREVPDILCSALTDNCIGRGKHLKEGGAVYDY
                     ISGLQVGIANLSDSLAALKKLVFEEKRLTTLEVWQALQSDYAGPRGEEIRQMLINEAP
                     KYGNDDDYADSLVRECYDVYVEEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGT
                     LATPDGRHAGTPLAEGCSPSHNMDKKGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTL
                     AKEEDKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAF
                     FNVLSKATQDDIIARTEHAL"
     gene            51100..51768
                     /locus_tag="VBE22_000351"
     CDS             51100..51768
                     /locus_tag="VBE22_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO7201829.1"
                     /translation="MEFLLDTLNLEAIKKWHHILPLAGVTSNPTIAKKEGDIHFFQRI
                     RDVREIIGREASLHVQVVAKDYQGILDDAAKIRQETDDDIYIKVPVTPDGLAAIKTLK
                     AEGYNITATAIYTSMQGLLAISAGADYLAPYFNRMENLDIDATQVIKELAQAIERTGS
                     SSKILAASFKNASQVTKALSQGAQSITAGPDIFESVFAMPSIAKAVNDFADDWKASQH
                     SEHI"
     gene            51836..52924
                     /locus_tag="VBE22_000352"
     CDS             51836..52924
                     /locus_tag="VBE22_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEO7201830.1"
                     /translation="MKVFASPSRYIQGKDALFQSIEHIKSLGQTPLILCDDVVYNIVG
                     ESFLSYLQDDLLPHRVSFNGEASDNEINRVVAVAKEKNSDLIIGLGGGKTIDSAKAIA
                     DKVNLPVVIAPTVASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVIAQAPK
                     RLLASGIADGLATWVEARAVLQKNGIAMAGGRQTLAGVAIAQACERTLFNDSLQALAA
                     CDAKVVTKALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALSGDIHHLTHGEKVAY
                     GTLTQLFLENRPKEEIDRYINLYQAIGMPTTLAELHLGDATYEELLKVGQQATIEGET
                     IHEMPFKISAEDVAAALLAVDRYVSNHQ"
     gene            complement(53076..54002)
                     /gene="cysK"
                     /locus_tag="VBE22_000353"
     CDS             complement(53076..54002)
                     /gene="cysK"
                     /locus_tag="VBE22_000353"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEO7201831.1"
                     /translation="MSKIYNSITDLIGNTPIIQLHHIVPEGAADVYVKLESFNPGSSV
                     KDRIALAMIEDAEQKGILKAGDTIVEPTSGNTGIGLAWVGKAKGYNVIIVMPETMSIE
                     RRKIIQAYGAQLVLTPGSEGMKGAIAKAKEISVEQNAWLPLQFNNQANPEIHEKTTGR
                     EIIETFGEKGLDAFIAGVGTGGTITGVSRALKKVNPDVAIYAVEADESAILSGEQPGP
                     HKIQGISAGFIPETLATDSYDHIIRVTSDDAIETGRIIGGLEGFLAGISASAAIYAAI
                     EVAKQVGKGKKVLALLPDNGERYLSTSLYDFE"
     gene            complement(54093..54737)
                     /locus_tag="VBE22_000354"
     CDS             complement(54093..54737)
                     /locus_tag="VBE22_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEO7201832.1"
                     /translation="MNYKTIKSDGIVEEEIKKSLFICHLKRVESEEEGRNYITQIKKA
                     HYKANHSCSAMVIGEKGDIKRSSDDGEPSGTAGIPMLTVLEKQGLTNVVAVVTRYFGG
                     IKLGTGGLIRAYSGSVANAIKEIEVVEVKEQIGIRIQLTYPQYQTFDNFLKEHHLQEF
                     ETEFLEAVTCKIYVDPKEFEHTITNLTEFYQGKALLTEEGSQIVEIPFKDETDK"
     gene            54793..56082
                     /locus_tag="VBE22_000355"
     CDS             54793..56082
                     /locus_tag="VBE22_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7201833.1"
                     /translation="MENYLGRLWTKAQLSEQLRKIAINLPSFIKKGSDYICTRCSSSV
                     AKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTFSKTNSLKWKGGLTPYQNEVSEE
                     LLKGISSKENLLVHAVTGAGKTEMIYHSIAKVIDTGGSVCIASPRIDVCLELHKRLSN
                     DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYHG
                     VKQALKENGTSIFLTATSTTELERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQK
                     SLQTQKLPPKLYQLINKQRQTRYPLLLFFPHISEGQVFTEMLRQAFPMEKIGFVSSKS
                     TSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVG
                     RALERPEGLLYFLHDGKSKSMHQAIKEIKNMNHIGGF"
     gene            56082..56747
                     /locus_tag="VBE22_000356"
     CDS             56082..56747
                     /locus_tag="VBE22_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEO7201834.1"
                     /translation="MTCLLCHEIDLSQLTFVELMLLKPKQNVICQTCKGSFEALSREM
                     GCQTCCKQIPQKQCQDCIYWGKKGIEVNHFSLYRYNEAMKKYFSLFKFQGDYLLKDVF
                     TKEIKAALKRYKGYTIVPVPLSHEGYQNRQFNQVIAFLQSANIPYKNILSKKDRGKQS
                     ANNKEERLKQVQQFTLKNEAELRDNLLIVDDIYTTGATIAQIRKLLEEKGIKNIKSFS
                     LAR"
     gene            56824..57378
                     /gene="raiA"
                     /locus_tag="VBE22_000357"
     CDS             56824..57378
                     /gene="raiA"
                     /locus_tag="VBE22_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEO7201835.1"
                     /translation="MIKYSIRGENIEVTEAIREYVETKLSKVEKYFNEEQELDTRVNL
                     KVYREKTAKVEVTILIDSITLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKYRE
                     KIPASQVFTTEFEAEPDEEAVSQRIVRTKNVNLKPMDVEEALLQMELLGHDFFIYTDA
                     EDNTTNVLYKREDGELGLIEAKGN"
BASE COUNT        19055 a         9111 c        11500 g        17851 t
ORIGIN      
        1 aaatgcgaca ttattgacaa atacattttt aatgccacta ttatatctag caatgttcat
       61 tgttccaatt ttgaataatg gtaaagagat tggtcgattt tttaatgaaa attattttgg
      121 gatagctttt ctagcaggaa tattaatagg tagtttatgt gtgatgccag ctagcattgt
      181 aggggtaggt atttctttag aaaaaagtaa tttctacttt ataaaaagtt tgcctatatc
      241 attttcgtat ttcttgaagc ataaatttgt aactttgata accttacaac ttgcagttcc
      301 tacttttatt tatttcttgg taggattctt tctacttaag ctatcaatcc tcgttttatt
      361 gagcttcatt ttaggtctag tcttcatggg acttatcgaa ggccagttta tttatagacg
      421 tgactataaa catctgttct tgaattggca agaggttacg caactattta atagaggggt
      481 aggccaatgg ctgttagttg gttcattgtt tggaatgatg ataataggta gttttcttat
      541 tggaataagt attttttggt caatggtatg gaatactgta gctgtcaata tcataatcct
      601 tattactggg ttgctcatat tatcaatttg tcaatatcta ctgctaaaaa atttttggaa
      661 gaagcttgtt tagtaaaaag attttagaaa aatcatacag taagaataat gcttaataaa
      721 tgagatagtt atagcggata aaataaaggc ttattgtgga ctttatggtt ggaaggcagc
      781 cactgtaatc ctttgtttaa taacgctagt tttagctcta aaatatttga tttttattgc
      841 tatctattat atcttatcag acaaagagac tagggataac ctagtctctt tgtctgatgc
      901 taacttgttt ttcgtaatat tgtgctagaa tgaatgtcaa tatagcagtt gtgcttacag
      961 taatgataac tgcaataagc atggtaataa aaagatgtgt tgaagtatga ctgatataaa
     1021 gaaacagacc tggtagacca gaagagccta cagcaaagcg gtgagcgttc attataccgg
     1081 catatagtcc tccaagacca ccaccaataa gtgaggctat tagaggataa cgtttcggaa
     1141 ggttgacacc atataagata ggttcggtga ttcccatata agctgttatg gcagcaggtc
     1201 cagcaatttc tttaagcttt ttttcttttg ttataagagt caccactgta cctgcagttg
     1261 cttgtgcgat atttgagcag agcatgcctg gtccaaaaat agaatcaaac cctaatttcg
     1321 ttagttgtgt gattcctaat gcagcaattc cactatgaac accaaacatg attaggatag
     1381 gagcaaatgc tcctactaga aaagcaggtg cccaactggc gtatttggat aaaaaagtaa
     1441 aaataacagc taaatattgg ccagcgatag tacccattgg tcccaatata gagaacgaca
     1501 aaattcccat gctgagaaat attaacatag gaacaagcac taaacgtagt gatttaggaa
     1561 taatttgatt taaatatttt tctatataag cctgaacaca aataattaga ataatgggaa
     1621 tgacacttga tgcatagtta gtaagtgtga atgggatcgt atggaataag gatactggtt
     1681 taccagaagc tactaaatta gcccaattag ggtgaaggag catgacaaca gttcccaaag
     1741 ctaaaatagg attagctttc aatttttgag cagctgtgat agcgattaag ataggtaaaa
     1801 aatagaaaac accatctgca aaaagattta gtaatatata tgtttgagat aacggtgtca
     1861 agattttgaa tactagtaat aaagctagca atgccttaat cataccagcg cctgataaag
     1921 ctggtaaaat cggttggaaa gtcccagaaa caaaatcaat aattcgacta actgctttgg
     1981 cttttttagt ttgttctgtt ttagtcgttg agttagtttc aatgagggag ttaatttctt
     2041 caaacacttt tgcgacatga gtcccgatca ctatctgatg ttgccctccg ctgataacga
     2101 cagtttttac ctcttttaaa gtttttaatt gctcaaggtt tactttcgtc ggatcattga
     2161 gactaaagcg aaggcgtgtt tgacaatggt aaacattgtt aatattattt tgcccaccaa
     2221 ggtgtttgat gatatttttt gacaaggcga tgtattctct agacataata tctccttaat
     2281 acttataaaa cgttttccga ttctagatta tagcatagta ataaaagaaa acacttggaa
     2341 atgcttgtga attttcaatg atgttttcag atagcattcc taaagggcct atgatataat
     2401 ttttttatga aaagacattt tgagacaact agaagaattg ttattaaagt aggaaccagt
     2461 tccctcgtac agacaagcgg gaaaattaat ttaagtaaaa tagaccattt agcatttgtt
     2521 atttctagtc ttatgaatag aggcatggag gtgattttag tatcatctgg agctatgggg
     2581 tttggtttag acattttgaa aatggataag cgtcctcaag aaatatcaca acaacaagca
     2641 gtttctagcg taggacaagt agccatgatg agtctttatt ctcagatatt ttcgcactat
     2701 caaactcatg tttcccaaat tttattaaca cgtgacgttg tggtttttcc agagagtttg
     2761 caaaatgtaa caaactcttt tgagagtctg ctctctatgg gaattttgcc aatcgtcaat
     2821 gagaatgatg ctgtcagcgt ggatgagatg gatcataaaa cgaagtttgg ggataatgac
     2881 cgtctctctg ctgtcgttgc taaaattact aaagcagatt tattaattat gctgtcagat
     2941 attgatggtc tttttgataa aaatcctaat atttatgatg atgctgtttt acgtagtcat
     3001 gtttctgaga tcaccgatga catcattaag tcagctggag gagcaggaag taagtttggt
     3061 actggtggca tgcttagtaa aattaaaagt gcacaaatgg tgtttgataa caatggacaa
     3121 atgatattga tgaatggcgc caatcctcgt gatattttaa aggtgttaga tgggcataat
     3181 attggaactt attttgctca aggaaagtga gattgagtta tgacgtatat tgaaatatta
     3241 gggcaaaatg ctaaaaaagc tagtcaatca gtagcacgat taagtacagc tagtaaaaat
     3301 gagatactca gggacttagc gagaaacata gtggctgata ctgaaaccat tttgactgag
     3361 aatgctcgcg atgttgctaa ggcaaaagat aatggtatct cagaaattat ggtggaccgt
     3421 ctacgactaa acaaagaccg catacaggcg atagcaaacg gtatttatca ggtagcagat
     3481 ttggcagatc cgattggtca agttgttagt ggttatacaa atttagatgg tttaaaaatc
     3541 cttaaaaaaa gagtgccttt aggggttatt gcaatgatat ttgaaagtcg tcccaatgta
     3601 tcggtggatg cctttagttt agcttttaaa acagggaacg ctattatttt acgtggtggt
     3661 aaagatgcta ttttttcaaa tactgccttg gttaattgta tgcgccaaac attgcaagat
     3721 acaggccaca acccagatat agtacaatta gttgaagata ctagtcacgt tgtagcagag
     3781 gagttaatgc aggcaacaga ctacgtggac gtcttaattc caagaggtgg tgcaaaatta
     3841 attcaaacgg tgaaggaaaa gtcaaagatt cctgttatcg aaacaggtgt tggaaatgtt
     3901 catatctata ttgatgaatt tgcagattta gatatggcag ctaagattgt tattaatgct
     3961 aaaacccaac gccctagtgt ctgtaatgct gctgaagggt tagttgttca tcaagctatt
     4021 gctaagggat ttttatctca attggagaaa atgctcaaag aatcgaacca gtcagttgaa
     4081 tttcgtgccg atgaggaagc tttacaactt ttagaaaatg ctgttgctgc aagtgagtct
     4141 gattatgcta ctgaattttt agactatatt atgtcagtga aagtagtaga ttcttttgaa
     4201 caagcaatta gctggattaa taaatacagt agtcatcact cagaagctat cattactaat
     4261 aatatcagta gagcagaaat ttttcaggat atggtagatg cagcggcagt ttatgttaat
     4321 gcttcaacac gttttacaga tggtttcgtt tttggactcg gagcagagat tggtatttcg
     4381 actcaaaaat tacacgcgcg tggaccgatg ggattagaag ctttaacaag tacaaaatac
     4441 tatattaatg gaactggtca agtaagggaa tagttgacct ataataggag agattgcatt
     4501 tatcggtctc tcttttactt ttctaaagtt taggatttat ggtacaatta aagttatgac
     4561 aaacgatttt catcatatta ctgtgctttt gcacgagact gtggatatgc ttgatattaa
     4621 gccagatggt atctatgtag atgctacttt aggtggggca ggacacagcg aatatcttct
     4681 ttcacaatta ggcccagatg ggcaccttta cgcatttgac caagaccaga aggctattga
     4741 caatgctcat atacgtttaa agaaatatgt ggataccggt caagtaacct ttatcaaaga
     4801 taatttcaga aatctatctt ctaacttaaa agcattagga gtgtcggaaa ttaatggtat
     4861 ttgctatgat ttaggtgtat caagcccaca gctggacgag cgtgaacgcg gtttctcata
     4921 taaacaagat gccccccttg atatgcgaat gaatagagag cagagcctta cagcttatga
     4981 cgttgtgaac acttatagtt atcatgattt agtaaggatt ttctttaagt atggcgaaga
     5041 taaattttca aaacaaattg ctcgaaaaat tgagcaagta cgagctgaaa aaccaatatc
     5101 tacaacaaca gaattggctg agattatcaa atcgtctaaa tctgccaaag aactgaagaa
     5161 aaaaggtcac ccagctaaac aaatttttca agctattcgt attgaagtca atgacgaact
     5221 aggagctgca gatgaatcta tacaacaagc aatggactta ttagctgttg atggtcgtat
     5281 atctgtcatc acttttcatt ctttagaaga tcgtcttact aagcaacttt ttaaagaagc
     5341 atcaacagta gaagtaccta aaggtcttcc ttttatacca gatgatttac aacctaagat
     5401 ggagctcgtt aatcgtaaac ccatcttacc aagtcaagaa gaactagaag cgaacaatcg
     5461 tgcccattca gcgaaattga gagtagcgcg tcgtattcga taataagaaa gaaacttatg
     5521 acaaatgaaa aacgaactga agcagtaaca caaactttac aacgtcacat caagactttc
     5581 tctcgcattg agaaagcttt ttatggcgct attgtaatta ctgctattat catggcagtc
     5641 ggtattattt acttgcaaag taatagttta caagttaaac aagaagtaaa tcaattaaat
     5701 agtaaaatca acgataaaca gacagaattt gacaatgcta aacaagaagt aaatgagctt
     5761 tctaatcgtg atcgaattac taaaattgct aaagatgctg gtttaacaat tcaaaacgac
     5821 aatatttatc ggaaggtgga ctagtctgtg acttttttta aaaagctaaa aaaaatcttc
     5881 cttgattatg tgatccatat aagagatagg cgttcgccac agaaaaatag ggaaagagta
     5941 ggtcaaaatt tgatgatttt aaccattttc ctatttttca tttttatcat taactttgtc
     6001 atcatcgttg gtacagatag taaatttggg gttaatttat ccaaggaagc caagaaagtc
     6061 tatcaacaaa gtatgacagt acaggcgaag cgtgggacaa tttacgaccg taatggaaat
     6121 cctatcgctg aggatgctac gacctatagt ctctatgcta ttatctcaaa gaactatacg
     6181 acagctacag gtcaaaaact atatgtacaa ccttctcagt atgaaaaagt agctagtatc
     6241 ttagagaata aattgggcat gaagaaaaat cttgtcctaa aacaactcaa tcaaaaaaaa
     6301 ttgtttcaag tttcctttgg aagttcaggt tcaggattgt cttataccaa gatggctgac
     6361 atcaagaaaa cgatggaaaa gtcagatatc aaaggaattg gtttttctac cagtccgggg
     6421 cgtatttatc caaatgggat atttgcttca caatttatag gatttactct tcctcaggat
     6481 gatggagatg gaaaaaaatt agttggtaat acaggccttg aagcggcact taacaaggta
     6541 ttatcaggta ctgatggtaa agtaacatac gaaaaagacc gtagtgggaa tgttctttta
     6601 ggtactgcaa caacagaacg tagggcggtt aatggtaagg atatctacac aactttatca
     6661 gagcctattc agactgtttt agagactcag atggatgttt tcgcagaaaa aaccaaagga
     6721 aaatttgcga gtgctacagt tgtcaacgct aaaacaggag aaattctagc aacgagtcag
     6781 cgtcctactt ataatccttc tacccttaaa ggttacgata agaaaaattt gggtacctat
     6841 aacacactac tatatgataa tttttttgaa cctggttcaa caatgaaggt aatgactcta
     6901 gcttctgcaa ttgattctaa gcattttaac tctactgaag tgtataacag tgcacaatat
     6961 aaaatcgcag atgcggttat tcgagattgg gatgtcaatg aagggttatc gtctggtagt
     7021 tatatgactt ttccacaagg gtttgcacat tccagtaacg ttggtatggt aaccttggaa
     7081 caaaaaatgg gacgagataa atggttaaac tatctttcta agtttaaatt tggttaccca
     7141 acacgttttg ggatgcttca tgaaagtgga ggtttgttcc catctgacaa tgaagtaact
     7201 atcgctatga gctcatttgg tcaaggtatt ggtgtaacgc aagttcaaat gttgcgcgca
     7261 tttacgtcta tctcgaatga tggtgttatg ttacaaccac agtttattag cagtatttat
     7321 gatccaaata cagggacttc tcgtacagca cgaaaagaag ttgttggaaa accaatttcc
     7381 aaagaagcag ctagtaaaac acgtgattat atggttactg ttgggacaga tccttattat
     7441 ggtacattat atgcagcagg tgccccagtt attcaggtgg gcaatcaatc agttgcagta
     7501 aaatctggta ctgcccaaat agctcaggaa ggtggtggtg gttatctgca aggaaagaat
     7561 gatactatta actcagttgt ggctatggtg ccatcggaaa atcctgactt catcatgtat
     7621 gttaccatcc aacaacccga aaaatttagt atcacattct ggaaagatgt tgttaatcct
     7681 gttttagaac aagcaacagc aatgaaagaa acgatattaa aaccgggatt gaatgactca
     7741 gaacatcaga caaaatataa actatcaaaa atagttggtg aaaatccagg tcatgtagcg
     7801 gaagaattac gtcgtaatct ggttcagcca attattttag gaaatggtag taaagtttct
     7861 aaagtatcta aaagaccagg agctaatctt gctgagaatg aacagctact agtattaaca
     7921 aataaactga ctgaattacc tgatatgtat ggttggtcta aagcaaacgt tgaacagttt
     7981 gctaaatgga caggtataaa agtgacctat aaaggttcaa cctcaggtaa agtaagaaaa
     8041 caaagtattg atgttggaaa atccatcaat aaaattaaaa aaatcaaaat tacaatagga
     8101 gattaacatg tttttaagta taatggcagg tgtcatagca tttgtcctga cagttattgc
     8161 cattccacgc ttcattaagt tttaccaatt gaagaaaatt ggcgggcaac aaatgcatga
     8221 agatgtcaaa caacatctag ccaaagcagg tacgccgaca atgggaggaa cggtattttt
     8281 gattgttgct cttcttgtct cacttatttt tagtattatt ttatctaaag aaaatagtgg
     8341 taatttaggt gcaacttttg gtattttatc tgttgttctt atctatggaa ttattggttt
     8401 tttagatgat tttttgaaaa tttttaaaca aatcaatgag ggcttgactc caaagcaaaa
     8461 aatgtcgctc caactaattg cgggtctaat tttttatttt gtccatgtgt taccaagtgg
     8521 cacatctgcg ataaatattt ttggttttaa ccttgaagta ggatacttat atgctttttt
     8581 tgttcttttc tgggttgttg gtttttcaaa cgctgtgaac ctaacagatg gaattgatgg
     8641 tctagcttct atttcggttg ttatcagttt gattacttat ggaatcattg cttataatca
     8701 aacgcaattt gatattttat tgattatcgt tatcatgatt ggtgctttac ttggcttttt
     8761 tgtctttaat cataaaccag ctaaagtctt tatgggagat gttggttcac ttgcattggg
     8821 tgctatgtta gcagccattt caattgcctt acgtcaggaa tggaccttgc tttttatcgg
     8881 ctttgtctat gtttttgaga cgtcttcagt gatgttgcaa gtagcatact ttaagtatac
     8941 taagaagaaa actggtgttg ggaaacgtat cttccgcatg acaccatttc atcaccattt
     9001 agaattaggt ggtgtgtctg gcaaaggtaa taaatggagc gaatggaaag tagatgcttt
     9061 tttatgggct attggtattt tcatgagcgc tattacactt gctattttat atctttaaac
     9121 tttattaggg gatgtggtat ggactgccag attccctttt gctttcttgt agcctttaat
     9181 ggtataatga acataataat agtaaggaaa tagacatgtc atttaaagat tttaatttta
     9241 aaccatacat tcagagggct ctcgatgagt taaaattcgt cgatccaact gatgttcaag
     9301 ctaaattgat tccagttgtt cgttcaggcc gcgatcttgt gggggaatcg aaaactggtt
     9361 ctggtaaaac ccacaccttt ttacttccta tttttgagaa gctagatgaa agtagtaatg
     9421 atgttcaagt tgttattact gcaccaagta gagagttggc aacacaaatt tatcaggcaa
     9481 ctaaacaaat tgctgagcat tcagagcaag aaatacgggt ggttaactat gttggtggta
     9541 ctgacaaatt acgtcaaatt gaaaagttga aagtaagcca acctcatatt gtcattggaa
     9601 ccccaggacg tatttatgat ttggtgaagt caggtgattt agctattcat aaggcgcata
     9661 cttttgtggt tgatgaagca gatatgactc tggatatggg attccttgat accgttgata
     9721 agattgcagg gtctttaccc aaggatgtcc aaattttagt cttttcagca acgattccac
     9781 aaaaattaca gccattttta aagaaatatt tgaccaatcc tgtaatggaa aaaatcaaaa
     9841 cagcaacagt tatcactgat accatcgata actggctttt gtccactaaa ggacgtgata
     9901 aaaatgcaca aattttggaa ctctctaaac taatgcagcc ttaccttgca atgatatttg
     9961 ttaatacgaa agagcgtgca gacgaattac actcgtatct ttctagtaat ggcttaaaag
    10021 tagctaaaat tcatggtggg attgcacctc gtgaacgtaa acgtattatg aatcaggtta
    10081 aaaacctcga atttgaatat atcgttgcaa cagatttagc agcgcgtggt attgatattg
    10141 aaggtgttag ccatgttatc aatgatgcca tacctcaaga cctatctttc tttgtccacc
    10201 gtgtcggtcg cacaggccgt aatggcttat ctggtactgc tataacttta tatcagccaa
    10261 gtgacgattc tgatattcgt gaacttgaaa aactaggtat taattttatt cctaaagtca
    10321 ttaagaacgg tgaatttcaa gatacatacg accgtgatcg tcgtaataac cgtgaaaagt
    10381 cctatcaaaa attagataca gaaatgattg gacttgtgaa aaagaaaaag aaaaaaataa
    10441 aaccaggtta caagaaaaaa attcaatgga aagttgatga aaagcgtcgc aaagagcgta
    10501 gagcaagtaa tcgtgctaag gggcgtgcgg agcgcaaagc taaaaaacaa agtttttgaa
    10561 attattaaag tctgaaatca gctcgtttca ggctttttta ttgatataaa atctttatca
    10621 cctgataagg aaatactatt atacagatag attttagagt gttagactag taatatgata
    10681 ttttaggaga attcttatgt ttaaaaaaat aatattatct attgcaacta tagtcgcaac
    10741 cgccagccta gcagtatcag ttcaggcgtc agagaaagta gaacttaaag tagctacaga
    10801 ttctgacacg gcaccattta cttatcaaaa agacgggaaa ttcaaaggtt atgatgttga
    10861 tgttgtcaaa gctgttttta aaggtagtaa gtacaaagta accttcaaga cagttccttt
    10921 tgatactatt tcaacaggta ttgatgcagg gaaatttgat ttatcagcta atgatttttc
    10981 atacaataaa gaaagagcag aaaaatatct cttctcagag cctatatccc gttcaaatta
    11041 tgccgtagta gggaagaagg ggagccatta caaatcatta agtgacctct ctggaaaatc
    11101 aaccgaagtt ttatctggcg ttaactatgc acaggttcta gaaaattgga ataaaaatca
    11161 tcctaataaa aaaccaataa aaatcaaata tgtttctggg acaactggtg ttactagcag
    11221 attaaaaaat attgagagtg ggaaaattga ctttatccta tatgatgcca tttcatctga
    11281 ctatattgta aaagatcaat cattaaactt aagcgtttct cctttgaaag gtaaaattgg
    11341 taataataag gatggattag aatacctcct tttaccaaaa gataaaaaag gtaaaactct
    11401 acagaaattt ataaataagc gtattaaagt tttgaaagaa gatggtactt tggcgcgttt
    11461 aagtaaacaa tatttcggtg gagattacgt ttcaaacatt gataagtaaa tcagtgagac
    11521 tataagtttt atctttttac atgtcagagt ttgcgtgata gaataacgaa gataagaaag
    11581 tttagaaaag gaaagtagaa ggaatgaatc aatttatctt gacgggtgga tggtcttggt
    11641 ataataattt ggttagtcag gttccagcgg ggaaattatt tagttggaag gctgttttag
    11701 atgcaatacc ctcaatttta gagcgattac ccataacact attattgaca gtagcaggag
    11761 ccttatttgg actcatttta gctttgattt ttgcaatagt gaaaattaac cgtgtcaaaa
    11821 ttttatatcc gattcaagct ttatttgtta gttttttacg tgggactcca atacttgttc
    11881 aattaatgct gagttattat ggaattccac ttttcttgaa atttttaaac caaaaatatg
    11941 gttttgattg gaacattaat gctataccgg cttctgtatt tgctattact gcatttgctt
    12001 ttaatgaagc ggcttataca agtgaaacta tacgagcggc gattttgtcg gttgatcaag
    12061 gtgaaattga ggcagcacgt agtttaggga tgacctcagc gcaagtttat cgtcgtgtta
    12121 ttattccaaa cgcagcagta gtcgcaacac ctactttgat aaatacgtta attggtctaa
    12181 ctaagggaac ctctttagct tttaatgctg gtattgttga aatgtttgcc caagcacaaa
    12241 ttatgggtgg tagcgattat cgctattttg aacgttatat ctctgtggca cttgtttact
    12301 gggcggttag tttcttaatt gaacaattgg gaaatgctat tgaacgaaaa atggcaatta
    12361 aggctccacg ccatttaact gatgagattc caggaggagt acgctaatga taaaattgag
    12421 acaattgact aaatcatttt ctggtcaaaa agtgttagac aaacttgatt tagatattga
    12481 aaaaggtcaa gttgttgctc tagtaggtgc ttcaggtgca ggaaaatcaa cctttcttcg
    12541 tagtatgaat tatttagaag agcctgatta tggaacaatt gaaattgatg attttaaagt
    12601 tgattttaaa tcaatcagta aagatgacat actaacatta cgccgtaagt tagccatggt
    12661 ttttcaacaa ttcaatctat ttgagcgtcg tacagctttg gataatgtaa aagaaggttt
    12721 gaaaattgtc aaaaagatgt cagatcaaga ggcaacacgg attgcaagag acgaactggc
    12781 aaaggtcgga cttgcagatc gtgaaaaata ttatccaaga catttatcag gtggacagaa
    12841 acaacgtgtt gctttggcac gcgcattagc catgaaacct gatgttcttc ttttagacga
    12901 accgacatca gcacttgatc cagaattagt tggagaagtt gaaaaatcta ttgcagatgc
    12961 tgctaagcag gggcaaacca tggtacttgt tagtcatgat atgaattttg tttaccaagt
    13021 agcagataaa gttttatttt tggaaaaagg tcgtatttta gagtctggga cgcctgaaca
    13081 attatttaac catcctttag aagaacgtac aaaagaattc ttcgctagtt ataacaaatc
    13141 gtacctataa gctaaaatat tttgatataa tgaaggttag aatgtctaat cttcattttt
    13201 aattattctt gagttaggct aagtatataa gaaagctgcg atatgttttg acaatcgctt
    13261 gttggataga gatgttttta gaaatagcaa aagtttattt acaaggttta gggcttgcca
    13321 gcataactgt tattttagtt ggtataattt gggtcttttg gcgtgccaag aaaaacatag
    13381 ataaaacagc tagagaacgc caatctgttc tttatgatgt ccttctgata gctcttatga
    13441 cagctccaat acttgccttt gcatttgtag ctatcatttt aatgataaaa gcttagtcag
    13501 attagttgga aatatagacg ctttgctata gaataaaacc ataagattca aaggagaaat
    13561 agtaatgtat gatactttaa taataggttc aggtcctggt ggtatgacag cagcgcttta
    13621 cgctgcacgt tcaaacctta aagttgggtt aattgaacaa ggtgctccag gtggacagat
    13681 gaataatact gctgaaattg agaactatcc aggctacgat catatctcag gtccagaatt
    13741 atcaatgaag atgtatgagc cgttggaaaa atttgaagta gagcatattt atggtatagt
    13801 tcaaagagtt gagaacgatg gtgatgtaaa gcgtgttatc accgaagatg aatcgtatga
    13861 agccaaaaca gtaatccttg ctaccggagc taaaaacagc cttttaggag ttccgggtga
    13921 agaggaatat actagtcgtg gcgtttctta ctgtgcggtt tgtgatggtg catttttccg
    13981 tgatcaggat cttttagttg tgggtggcgg tgattctgct gttgaagaag ctgtcttctt
    14041 aactcaattt gctaaaagtg ttactattat tcaccgacgt gatcaattga gagctcaaaa
    14101 agtacttcaa gaccgagctt ttgctaacga aaaaataaaa tttgtatggg attcagtggt
    14161 caaagagatt aaaggaaatg agattaaagt atctggtgta actgttgaaa atctaaagac
    14221 tggagaaatc tcagagatgc ctttcggagg tgttttcatt tacgttggtt tgaaaccaca
    14281 ttctagcatg gtatcagaat taggtattac ggatgagact ggctgggtct taactgatac
    14341 aaatatgaaa acatcaattc caggactata tgctattggg gatgttcgtc aaaaagacct
    14401 acgtcagatt gcgactgctg ttggtgaagg tgctatcgct ggacagggtg tctataacta
    14461 tataactgag catttctaag aaatacaaag tagaaaagac tatcgaaaaa cgattagtct
    14521 ttctttttat tttcatgaaa gtaaggtgat aaactgaact accgagatag aaatatgcta
    14581 taatgttaga catatagcct tatcctaggc tcatgattaa aaggagattg tctgatgtat
    14641 aaagacgata gtttaacact acatactgat ttatatcaaa tcaatatgat gcaagtttat
    14701 tttaataaag gtattcacaa taaaaaagcg gtgtttgaag cttactttcg caaggttcca
    14761 tttgaaaatg gttatgctgt ttttgcaggt ttagagcgta ttgttcgtta tttagaaaat
    14821 ctgagttttt ctgatagtga tttatcctat ttagaagagt taggttatcc tgaagaattt
    14881 ttagattact taaaaaattt aaaaatggaa ctgactgtca aatcagccaa ggaaggtgac
    14941 cttgtatttg ctaatgaacc attagttcaa attgaaggtc ctttagcaca atgtcagtta
    15001 gtagagacag cgattttaaa tatcatcaac taccagacac ttgttgctac aaaagcagct
    15061 cgtattcgtt ctgttattga agatgaaccg ctattagagt ttgggacacg acgtgctcaa
    15121 gaaatggatg cagccatttg gggtactagg gcagcgatta ttggtggtgc taacgcaaca
    15181 agcaatgttc gtgctggtaa aatctttaat attccagtat caggaacgca tgcacatgct
    15241 ttagttcaaa cttatggcga tgattaccag gcatttaaag cttacgcaga aactcataaa
    15301 gattgcgtct ttttagtgga tacttatgat acccttcgtg taggggtgcc taatgctatt
    15361 cgagttgcaa aagagatggg cgaaaagatt aacttcttgg gagttcgcct tgattcaggt
    15421 gatttagctt atttgtctaa gaaagttcgt cagcagttag atgacgccgg ttttcctaat
    15481 gcaaagattt atgcttcgaa tgatctagat gaaaatacaa tcctcaacct aaaaatgcaa
    15541 aaagctaaaa ttgatgtttg gggtgtcggg acaaaattaa ttacagccta tgatcagcct
    15601 gcgctaggtg ctgtttataa aattgtttct attgagactg acgccggtag tatgcgagat
    15661 acaattaaac tatcaaacaa tgctgaaaaa gtatcaacac ctgggaaaaa gcaagtttgg
    15721 cgtatcacca gtcgagctaa agggaaatct gagggtgatt acatcacatt tgcagacact
    15781 gatgtgacac aattagatga aattgacatg tttcatccaa cttatactta tatcaataaa
    15841 acagtccgtg attttgacgc agtaccctta ctggtagata tctttgacaa agggaaatta
    15901 gtttatcaat taccaagtct tcaagaaatt caagagtacg gacgcaaaga atttgatcag
    15961 ctttgggatg agtataaacg tgttctgaat ccacaggatt acccagttga tttggcacgc
    16021 gatgtttggc aaaataaaat ggacctgatt gatcgtatcc gtaaagaagc tttggcaaaa
    16081 ggagaagtaa gatgactttg caagatcaaa ttatcaaaga actcggtgta aaacctgtta
    16141 ttaatccaag tcaagaaatc cgtcgttccg tagaattttt aaaagactat ctattaaaac
    16201 atagttttct aaagacctat gtactgggta tttcaggggg gcaagattca actttagcag
    16261 gacgtctagc tcaactagca gttgaagagt tgcgtgctga tacaggggaa aactatcaat
    16321 ttatagctat tcgtttacca tatggtattc aggccgatga agaagatgca caaaaagcat
    16381 tagattttat caagccagat attgctttga ccattaatat taaagaagct gttgatggcc
    16441 aagttagagc tcttaatgca gcaggagtag agattacaga ctttaataaa ggaaatatta
    16501 aagctcgtca aagaatgatt agtcaatatg cagttgcagg tcaatatgct ggagcggtta
    16561 ttggtactga tcatgctgca gaaaatatta caggtttttt caccaaattt ggtgatggag
    16621 gtgctgattt attacccctc tttagactta ataaaagtca gggaaaacag ttattagctg
    16681 aacttggtgc tgataaagcc ttgtacgaaa agattccaac cgcagattta gaagaaaata
    16741 aaccaggaat cgctgatgaa atagcacttg gagtaaccta ccaagaaatt gatgcttatc
    16801 ttgaagggaa ggtagtttcg gacaagtctc gagatatcat tgaaaattgg tggtataagg
    16861 gtcaacataa acgtcatctt ccaatcacaa tttttgatga tttctggaaa taagaaaaat
    16921 agctagaata ttctagctat ttttgtttct ctaataaatt actttcagac ttgcagaaat
    16981 attcagaatg tttataatag tagctaagta taaaaggagg aatttatgtc agaattaact
    17041 caaacattta cagataagtt atttgctgat taccaagcaa atacaaaatt tagcgctatt
    17101 gaaaatgcag taactcacaa tggtttattg aagtctctag agacacgtca aagtgagatt
    17161 gagaacgact atgtcttttc aattgatttg accaaagatg aagtctctaa tcagaaacaa
    17221 tcaggacgtt gctggatgtt tgctgcactg aacacatttc gtcataaact gatttcagat
    17281 tttaaactcg agaactttga attatctcag gcacatacat ttttctggga taagtatgaa
    17341 aaatcaaatt ggtttatgga acaaattatt gcgactgcaa atcaggaatt gagtagtcgt
    17401 aaggtgaaat tcctattaga tgtacctcaa caagatggcg gacaatggga catggtggtt
    17461 gctctgtttg agaaatatgg tgtcgttcct aaaacagttt atcctgaatc tgtttcatca
    17521 agtgctagtc gtgaactaaa tcaatatttg aataaactct tacgccaaga tgcacagatt
    17581 ctacgagaat taatagcaca gggagcagat ggggcaactg ttcaaaataa aaaggaagaa
    17641 ttgcttcaag aaatttttaa ctttttagca atgaaccttg gcttgccacc gcaatctttt
    17701 gattttgctt atcgtgataa agacaatcat tatcagtctg ataaaaatat tacaccgaaa
    17761 gcattttatc aaaaatatgt taatcttgac ctatcagatt atgtttctat tatcaatgct
    17821 ccaactgtag ataagcctta tggacaatcc tatacagttg agatgcttgg aaatgttgtc
    17881 ggagggccag ctgttaaata tttaaacctt gatatgaaac ggtttaaaga gttagcgatt
    17941 gcgcaaatga aatcaggaga aactgtgtgg tttggttcag acgtaggtca agtatcaaat
    18001 cgtcaaaagg gtatcctagc aactacgact tatgatttta attcatctat ggatattaaa
    18061 cttagccaag acaaagcggg ccgcctagat tatagtgaaa gtttaatgac acatgctatg
    18121 gtcttaacgg gagtagacct tgatgaatct ggacaacctt taaaatggaa agttgagaac
    18181 tcttggggag agaaagttgg taaggatggt tattttgtag cttcagatgc ttggatggat
    18241 gaatatactt accaaatagt cgttcgtaaa gaattgctta ctaaagaaga attagaggct
    18301 tacaacgcag agccaattac tttagcgccg tgggatccga tgggagcact agctaattag
    18361 taatagtaat tataaattaa aaaactggat atattatcca gtttttttaa ttaccgttag
    18421 gtgctgtatt gctgttacca ttaccatgtc cagttcctgg cacagtttga ttagaattat
    18481 taggggtagt agcattacca ttattaggtg tttcgacttg tggggatgat gaattggcat
    18541 catttgctgt attcttatcc tctttttgtt tctcagagga tgttgaacta ctgctttgac
    18601 ttgactgtcc agaattttga tagatattct tatagacaga agaagagtaa gtgccggttg
    18661 tagttgtccc attgatatac aagtagcttc cgcttcgata gagcccttct ggcattgtcc
    18721 aatctgcact gtatccgcca gttaggtagg acatcattgc acgataaacc tcagtagcaa
    18781 tatccagttg gctaccataa agtggtgtaa ggcgattttt ataaccagtc caaattgcca
    18841 ttgtgtactt agaagtatag ccgacaaagt tttcatcagg agccattgta ccaacggcgc
    18901 tattgtagat accagtagtt gcttcaattt tagctaactc atcttccgta tagttggaag
    18961 taccagtctt accagcttgt gcaacaccag ggatagctgc tttagtacca gtaccaaatg
    19021 ttagtactgt tttcagcata tccgtcatca tgtaggcagt agtctctttc atcgcacggc
    19081 taccagacgc tgcataagta tcattggttc catcgctaaa ttcaatttta ttaacatatt
    19141 gcggcttata gtaagttccg ccgtttgcaa aagccgagta agccgctgcc attttctcac
    19201 tacttgcacc atattttgca tcactgctac tgttgttact tgaaatagca tttgaatagt
    19261 tcatttctgg atagtatatg cctaattttt caaggaaaga ttttgcttcg tctaatccag
    19321 cggcttcaag cgctctgaca gcaggcacgt tacgtgattg ttgaatagcg gtctgcatac
    19381 tcatccaacc catatattga cgatcccagt catatagttg agtagaagta ccaggccagt
    19441 aataaactga gtcgtttaat gattgacctg ttgaattata gacaccacta tcaatagcag
    19501 gtgcataagc tgagataggt ttcattgtag aaccccaatc gcggtctgtt aagacagatt
    19561 gatttgtccc aaatgaaata ttttcattct gatgacgccc gcctaattgt gcaatgactt
    19621 taccattagt cgcatccatg atggtagatg ctatttgtaa ttcattgttt ggataagcga
    19681 tgtaagtgtc actgttgtag atgtcatata gttgtttttg tgcatcagtg ttgatattag
    19741 tatacacttt tagcccagca gtaaagatat ctttaccagt tttttgttta acttcactaa
    19801 taacttgttt taagtagtta tccatatatt ttggataagt agatttttgc tttaattctt
    19861 ttaagccatc agttactgga gttgcaacag cttggtcgta ttccttttta gaaatgtttt
    19921 tatcttgata catctgctga agaacggtat tacgtcttgt ttgagcagat tctgggtttt
    19981 tataagggtc atattgtgta ggtgcttgag gaataccagc gagcaaagca agttgtgcaa
    20041 tagataattc ctttaggtct ttaccaaagt atgatttagc tgtcgttctc ataccataat
    20101 taccatttcc catataaact ttattaatat agaaagtaag aatttcttct ttggtgtatt
    20161 tacgctccat ttgaagcgca agccaaactt cctgtgattt acgttttaac gtttggtcag
    20221 atttattggt agaaaagtaa gccagtttaa tcaactgttg atcaagggtt gaaccacctt
    20281 gcgtattact actaacaaga ttatgccaag ctgcacctaa aatacgataa atatcgacac
    20341 ctctatgttt aaagaaacgt ttatcttcga tagaagtgat agcgttaacc aaatttagtg
    20401 gaatgctatc cgcactaaca ctttcacgct tttctgagcc taaatcggca ataagtttat
    20461 tattaccatc ataaaccaaa ctagagttaa cggattttaa agcttgatcg gttaatttcg
    20521 gagaacgact aacgtagtat gcaaatagga gcccacctat tacaatagtt aagataataa
    20581 atcccattat aatgccaaaa gcatacttca atagtttgat aacagattcc tttttaattg
    20641 taatcaccgc ctaataattt ttcttcaata atatctaagt aagggacttg aggaaaggca
    20701 ctctccttta cgaaaaatcc attttttctg atataatcaa taggcattga tttattgcct
    20761 ttatcaatct gataaaatga aattaactca tgagcaggga gtagataggt ttccttaagt
    20821 gtggaaaaat gaagcaagac aaagcaaatc cctttttgct gtaatacatt tgccatgtgc
    20881 tctatttggt gagcatgaaa atttttcata ggcatagcag ttttctgccg ggtttctttg
    20941 gcttcaaagt caatatagta acctttgtaa acaccagaat aatcagtagt agaagcttgt
    21001 ctaaaataag cttctacaat cttagcacga ctacgtttag gataatctac cttgacgatt
    21061 tgtactggcg ttggtttctt atgaatgaca gctaattcgt gagacaaata gtagtcattg
    21121 gttgcattga tagcagcttc aaatgacata ccacggttgg caaaatcgat tttctttttc
    21181 tttgattttg taactgttgt tttccgaata agctgatgtg gatagttaac catttttctc
    21241 cttgcattac aaataaaatg ctaagataag caaaactatt ataccataaa attaggatgg
    21301 acaacaaatg aagtcaacta ttcttgtaac aggttacaaa aattttgaat tagggatttt
    21361 ccaggataag gatcctcgga ttactattat aaaaaaggct attgataagg attttagacg
    21421 cttcttggaa aatggggcag actggtttat ttttatggga aatttagggt ttgagtactg
    21481 ggctttggaa gttgctcttg atttacaaaa agaatatgat tttcaaattg cgacaatttt
    21541 tacctttgag aaccatggcc aaaattggaa tgaggctaat aaagctaagt tggcactctt
    21601 taagcaagtt gactttgtta aatatacttt cccaagctat gaaaacccgg gtcaatttaa
    21661 acaatacaat cattttctca ttaacaacac acaaggtgca tatctttttt atgattctga
    21721 aaatgaaact aatttaaaat ttttgctaga aatgatgaaa aaaaaggaag cttatgatat
    21781 ttctttccta acttttgatc gcttaaatga aatatatgag gaatgatcgt tttttcggat
    21841 atgagaacgt tttacgcttg atttttccat ttaatttagg tattatttac tttgatataa
    21901 aggataggac ttatattaaa acgatttgac tggtttggag aatagaatgg caagtattat
    21961 ttatagccca aaagatattt ttgagcaaga ttttaaagtt agtatgcggg gatatgacaa
    22021 gaaagaagtc gatgtatttc tggatgatgt catcaaagat tacgaaaatt atcttgagca
    22081 aattgagaag ctccaaatgg aaaatagacg tctacaacaa gctttagaca aaaaagaaag
    22141 ggaagcttca aatgtaagaa attcagggac agctatgtat aatcaaaaac cgattgccca
    22201 atctgcaacc aattttgata ttttaaaacg cattagccgc cttgagaaag aagtttttgg
    22261 aagacaaatt agagaataaa gatttctcaa atatgcaatt tttggataat cgcgtagtat
    22321 tgatatacta tgaggaaagt ccatgctagc actggctgtg atgccagtag tgtttgtgct
    22381 aggcgaaaaa ataagcctag ggagatagct agctatctta cggcaagcaa aagggctaag
    22441 tctttggata tgcctgaata gctttgaaag tgccacagtg acgtagtttc tagggaaatc
    22501 tagaaagtgg aacgcggtaa acccctcaag ctagcaaccc aaactttggt aggggcatgg
    22561 gatagttgga atgagaacaa tctatcctga ctgctttgca gtagacagat gattatcgaa
    22621 agaaacaatt cctagttgtt tctggaacaa aacatggctt atagaaaatt gcataatagg
    22681 ttagtggctg agataacatc tcagccaatt tttaactgtg atgaatacga tagtgtaaaa
    22741 gaggaaaaat gaaagaatca ttcaaattaa tagcaactgc tgctgctggt ttagaagcca
    22801 ttgttggtag agaaatcaga aacctaggaa ttgactgcca agttgagaac ggtagagtcc
    22861 gtttccacgg agatatcaaa acaatcattg aaacgaacct atggctacga gctgccgatc
    22921 gtataaagat tattgtagga gagtttccag cacgtacttt tgaggaactt tttcagggtg
    22981 tatatggcct tgactgggaa aattatttac cgttaggggc aaaatttcct attgctaaag
    23041 caaagtgtgt caagtcaaaa ttgcacaatg aacctagtgt acaagccatt tctaaaaaag
    23101 ctgttgctaa gaaacttcaa aaagtctttc accgtcctga aggagttccc ttacaagaaa
    23161 atggagctga atttaaaata gaagtttcta ttttaaaaga taaagcgact gtcatgattg
    23221 atacgactgg atcaagtctc ttcaaacgtg gctaccgtgc agaaaaaggt ggtgcaccaa
    23281 ttaaagaaaa catggcagca gctattattc aattatctaa ttggttccct gataagccat
    23341 tgattgatcc aacttgtggt tcaggtactt tttgtattga agctgctatg attggaatga
    23401 atatagctcc tggttttaat cgtgattttg cctttgaggc gtggccatgg gtagatcaaa
    23461 gtcaagtaca aaaggttcgt gatgaagctg agagtaaagc aaactatgat attgatttag
    23521 atatttcagg ttttgattta gacggtcgta tggtagaaat tgctagaaaa aatgcagaag
    23581 aagcaggact tggagatgtt atcaaattga aacaaatgcg tcttcaagat ttaaaaacag
    23641 ataaaattaa cggtgttatt atctccaacc caccatatgg tgaacgactc ctggatgaca
    23701 aagcagtgga cattttgtat aatgagatgg gacaaacatt tgcgccttta aaaacttgga
    23761 gcaagtttat cctaactagt gacgaaggat ttgaaaaaaa atacggctca caagctgata
    23821 aaaaacgtaa gctttacaat ggtactttaa aagttgattt gtaccaatat tacggtgaac
    23881 gtgtgagacg tcaagtaaaa taacggggag aactcatgtc agaagatcaa aaacacccat
    23941 tttttgaacc agaaaaagaa actgatggtc ttgaatttaa agatgctaaa gaaatgacag
    24001 tagaagaggc tgttagaaaa gattctgaaa ttaaggctgg tattaccgaa gaagatagta
    24061 ttcttgataa atacatcaaa caacatcgtg atgaagtagc ttcacaaaaa tttgagacaa
    24121 aatcaagcga ttttgcaaac ctcgatacgg catctttaga tgattttatc aagaaacagc
    24181 gcgaagaatt atcagcgatg ttagctgcag aagagctttc taaaaagttg gataattcag
    24241 tctctcaaga acaagataca gaagctaatg ctgtctcacc taaagaagaa tcttcacaag
    24301 aacaagaaaa tagtgtaacc ccagttcccc ctctgattac ggaagcagaa ccaactgcaa
    24361 cagaaccaga ttcgactatt gctgattcag aggagtataa atcctcttct aggaagcgtg
    24421 gaggtatcgt aggaacattg attgctctta ttttacttct aatagtagca atctttggtt
    24481 acaactattt caagaataat aattcgacaa attctcaaac tgcaacaagt caaagttcta
    24541 gttctaaggc tacgacgact tcatcagaag aagataaaaa agctagtcag aacttagata
    24601 atttcaacaa agcttatgct aacttttttg ttgatgataa gaaaacccaa ctcaaaaata
    24661 gcgaatttga taaattgtca gaattagaaa agaaagttga tgctttaaaa ggaactaaat
    24721 actacggtaa agtaaaagtt aaatttgata gtctaaaacg tcaaattgat gctgttaaag
    24781 ctgttaacga caagtttaag tcgcctgctg tagttgatgg taagaagagt gaaaaattag
    24841 aagtaaaaga tggagcgaat tttgactctt tagatagtaa aactcttaat actggtaatg
    24901 ctagtctaga tagtttactt cactccatcg tttcaactgg tcgtaatcag gtaaaacaat
    24961 ccgaagaaca ggcttcgtct aataaggttt cagacactca aacaactgaa caaccaaacg
    25021 taactaacgg tcaatcttcc tcatcagctg ctacaataaa taatcaggca gctggcactg
    25081 catcaggtaa tttggaacgt aaccgtagcc gagtaccgta taataatgct gctattgcgg
    25141 atacaggaaa tcctgcctgg atatttaacc caggagtatt agagaaaatc gtggccactt
    25201 cacaggcgcg aggctatttc tctggaaata actatattct tgaacctgta aatattatca
    25261 atggtaatgg ttattacaat atgtttaaat cagatggcac ttatttgttc tctatcaatg
    25321 ccaagacagg ttactttgta ggtaacgctc ctggaagagc tgatagttta gattattaat
    25381 ataaagaaca aaaccagttt tttgtctaca ccctaaaagt tagaaaatca gtctaacgat
    25441 tagggtgttt tttgtattcg catttagatg tctacgactg ctatgaggat attagggaaa
    25501 aattaaaagg attttgctct ctatgataca gaacaaaatc ctttgtttaa attaatagga
    25561 caacttttgg aattccaaaa gttgactttt attttttatt agatcagaca atgtggcgct
    25621 caaaagcatt gtcagaaatt ccttgggaaa gaattaattt cgcccattct tgagcagagt
    25681 ggagactgtg gtctttataa tttccacaag attcaatggt tgttccagga acgtcttccc
    25741 aagtgacccc accagcaata gcctctagac tagatttgat gactttagca atttcagtag
    25801 catcctgttt tccccacata atcatgtgaa aaccagttct acaaccaaaa ggagaacaat
    25861 caatcaaacc attaattcgt tggcggatta acttcgccag aaggtgctcg atagtatgta
    25921 aaccagcagt atcaatagca ttttcattag gctgaataag gcggatatca aagttagtaa
    25981 tgatgtctcc gaccggtcca acttcttctg aaattaaacg aacgtagggt gctttaacga
    26041 tggtgtgatc taattcaaaa ctctctacga caacttcttt tgtcataagt tttctccttt
    26101 tgtattacat tctagagact aaaggagatg ttgaaaattt ctcctaatcc ctttaaataa
    26161 cattctaatc atatcataaa tgtctataat tttaaattgt gaaaacgcct tttttatgat
    26221 aaacttaaaa gtggactttc tatatgaaat ctagaaaaaa attgaggtat aaacatgttt
    26281 aatattatat tagcaatggt ttgtgcactc attggtttga ttattggtta tgtggctatc
    26341 tcaatgaaaa tgaaatcatc aaaagaagct gcagaactaa ctcttttaaa tgcagaacaa
    26401 gatgccgttg atttacgtgg taaagcagaa attgaagcag aacacatccg taaagcagcg
    26461 gaacgtgaaa gtaaagctca ccaaaaagaa ttacttttag aagcaaaaga agaggctaga
    26521 aaatatcgtg aagagatcga aaaggaattt aaatctgata gacaagagtt gaaacagatg
    26581 gaggcacgtt taacagatcg tgcatcatct cttgatcgta aagatgaaaa tctatctaac
    26641 aaagaaaaaa tgttagatag caaagaacaa agtctaaccg ataaatctag acatattaat
    26701 gagcgcgaac aggaaattgc tactttagaa acaaaaaaag tagaagaatt gtcacgcatc
    26761 gcagagctta gtcaagaaga agctaaagat atcattttag ctgataccga aaaagattta
    26821 gctcatgata ttgcgacacg tattaaagaa gcagaacgtg aagtaaaaga acgatcaaat
    26881 aagatcgcaa aagacttgct agcccaagct atgcaacgtt tagctggtga atatgttact
    26941 gaacaaacaa ttacaactgt tcatctccct gatgataata tgaagggacg tatcattggt
    27001 cgtgaaggac gtaatattcg tactctggaa agtttgacag gtattgatgt tatcatagat
    27061 gatacgccag aagtcgttgt cttatcaggt ttcgacccca ttcgtcgtga aattgctcgt
    27121 atgactttag aatctttgat tcaagatgga cgtatccatc cagctcgtat tgaagagttg
    27181 gttgaaaaaa atcgtttgga aatggatcaa cgtatccgtg agtatggtga ggcagcagct
    27241 tatgagattg gtgcaccaaa tctgcatcct gatttgataa aaattatggg acgtttgcag
    27301 ttccgtacat catatggtca aaatgtactt cgtcattcag ttgaagttgg taaacttgca
    27361 ggtattttgg ctggagaact aggagaaaat gttgatttag ctcgtcgggc tggattcctc
    27421 catgatatgg ggaaagctat tgatcgcgaa gttgaaggca gtcacgttga aattggtatg
    27481 gaatttgctc gtaagtacaa agagcatccg attgttgtta atactattgc aagccaccat
    27541 ggtgatgtag aacctgacag cgttattgca gtaattgttg ctgctgcaga cgctcttagt
    27601 tcagcacgac caggtgcgcg taatgagtca atggaaaatt atatcaaacg tctgcgtgat
    27661 ttagaagaaa ttgctaatgg ctttgaggga gtgcaaaatg cctttgcact tcaagccgga
    27721 cgtgaaattc gtatcatggt acaacctggt aaagtttctg atgatcaagt agttattatg
    27781 tcgcataagg tacgtgaaaa aatcgaacaa aacttggatt atccaggtaa tattaaggta
    27841 acagttattc gtgaaatgcg agctgttgat tttgcaaaat agtcaaaagg agagcttgtt
    27901 agctctcctt ttgttataga gttgacattt gtcatatttt gaaatgacaa aaatatctaa
    27961 aaagtttagt tctgattttt tatactatga ttatagtaaa ggagaaccaa atggcaaaag
    28021 atataattaa ggttgatcat atatttaaaa gcatcgggca aaaaacgatt ttagaagata
    28081 tatcatttag tatagcttct aaccaatgtg tagcattgat tggacctaat ggtgcaggga
    28141 aaacaactct gatgtcaact ttattaggtg atattagtat ttcttcagga gcattgacga
    28201 tttttaatct gcctgcacac cacaataggt taaaatataa gatggccatc ttaccgcaag
    28261 aaaatgtatt gccctcaaag tttacagtga gagaactcat tgatttccaa aggtgtttat
    28321 ttccagaagt gctgccaatg tctttgattt tagactatct acagtggagt gatgcgcact
    28381 tgcaacaatt tacagagact ttatctggag ggcaaaaacg acttttagct tttgttttaa
    28441 ctttggttgg taagccccaa ttactatttt tagatgaacc tacttccgga taagttatca
    28501 gactttggtg atagaatcac gtttctaatt gttcctattt tttgtatcgg ttacatgtgg
    28561 attgctattg aaaatagaaa tagtgaagaa caaagagaaa aaattgcaga gcaaaatcag
    28621 tatatcaata ttttgtcagc tgaaaatgaa cgtaaccgta tagggcgtga tttgcatgat
    28681 agtttaggac atacatttgc tatgatgaca ctaaagacag aactagcgtt aaagttgtta
    28741 gaaaaaagga actatgataa ggtacaaaaa gaactatcag aattaaatca tattagtcat
    28801 caatcgatga gtgaggtccg tcaaattgtt tctaacctca agtatcgtac agtagtggaa
    28861 gaaattgatg agttgtaccg cttgtttcaa ttatctaaca tcaagttaac agttgttaat
    28921 aagttagaga cgtcacaact aagtccagta acacaatcta caatcacaat gatattaaag
    28981 gaattatcca ataatattgt taagcatgct gaagctgata gcgtggagtt gagtttagta
    29041 aggcaaggtg ctactataaa tattgagatg attgataatg gttgtggttt tacaaacctt
    29101 gacggagatg agttgcatag tattcaagag agattaacta tcgttgaagg gacactaacg
    29161 attttgtctc gaagtaaacc aactcatatt caggtggtat tgaaagaggt ataagatgaa
    29221 attacttgtt gctgaggatc agtccatgct acgagacgct atgtgccaat tacttttgat
    29281 ggaagaaagt gtttctacta ttgatcaagc tggaaatggt gaagaagcta ttgccatttt
    29341 atccaataaa gccatagatg ttgcaatatt ggatgttgag atgccgatat tatcaggttt
    29401 agatgtctta gagtgggtta gaaaatacca gaatgttaag gtcataattg taacaacctt
    29461 taagcggtca ggttatttcc aaagggctat tcgttcaaat gtagatgctt atgttttgaa
    29521 agaccgctca gtagctgatt tgatgaaaac gattcaaaaa gttttatctg ggggtaaaga
    29581 atattcgcct gaattaatgg aaaacgttat atctaatcct ttgagtgagc aagaaataaa
    29641 aattctttcc ctaattgctc aagggaagac cagcaaagaa attgcatgca ctttattttt
    29701 atcgaatggt acagttcgta attacacgtc aaatattttt gataaattag gagttaataa
    29761 tcgtttggag gcacttaaaa tagcacgaga aaaggattgg ctgtgatagt ttttttccag
    29821 tcgagatgcc ataatgtatt aagagggata tatgtatgca aaaatttaaa gaacgttttg
    29881 atacgcttag tgatgcaatt ttggccattg ctatgacgat tttagttttg gagataaaaa
    29941 ctccggctac gatgggtgat attggtgact ttacacggaa cataggttta tttattgtta
    30001 gctttgttgt tgtttttaat ttttggtacg aaagggcaca aaatagccta gatgctcaaa
    30061 agactaatga tgaaattata gctcttgata tcattgaaca cctaggtatt tgtttgattc
    30121 ctctctttac taaatttatg atttcttttg agaatcataa ttttgctgtt atggcttatg
    30181 gactcttaac gttattagtt ggtctcacat cagacattat tcggattcat ttggcaagtt
    30241 atgatttagt gaccattcct agtgaattga aagagcgggt tataaaagtc atgacaactt
    30301 ttgccattcg ttcagttgtg gttcgcttta tcattattat ccttgcttat tttctacctg
    30361 aggtagggat atttgcatat ttagtcatac cgctctttat gttttggcgt cgctctagag
    30421 caggaaaata ctatgctaaa aagggcattg aggcaccctc ttatatcaag ctttttatcc
    30481 aaattggaga gagagttcct gaggatttta ctaattcaag tagtggaata ggtggggtaa
    30541 aaagactcta taaaaaataa taggagacct tgaaaaaaag gtttcttttt gttaaactaa
    30601 tgagaagaaa ctttatttag ggagcaattt atgaattgct ataaaagtta atagttaagg
    30661 taagaaccag attgttctgg tctttttaac acttaacacc cgatctatta atttgaggag
    30721 tgtgtaatgt ctgaacgtgg tttgctaatt gttttctcag gaccatctgg tgttggtaag
    30781 gggacagtta ggcaagaaat tttttcaacc cctgaccata aatttgatta ttccgtttca
    30841 atgactactc gtccacaacg cccaggcgaa gttgatggag ttgattattt cttccgtaca
    30901 cgtgaagagt ttgaagcgtt gattaaagaa ggtcaaatgc ttgagtatgc ggagtatgtt
    30961 ggtaactact atggaactcc tttaagttat gtcaacgaga ctttagacaa agggattgat
    31021 gtttttcttg aaatagaagt tcagggagcg cttcaagtta aatcgaaggt tccggacggt
    31081 gttttcattt tcttaacacc accagattta gaggaattag aagagcgttt agtaggacgt
    31141 gggacagata gtccagaagt gattgcccaa cgtattgaac gtgccaaaga agaaattgct
    31201 ttgatgaggg agtatgacta tgctgttgtc aatgatcaag tgtcattagc agcagaacgt
    31261 gttaaacgtg tcattgaagc agaacattat cgtgtggatc gtgttattgg gcgttatacc
    31321 aacatggtca aagaaactga taaaaaatta agttaaaaat agaaaagaga attgccttat
    31381 gatgttaaaa ccttctatcg acactttgtt ggacaaagtt ccttcaaaat attctttagt
    31441 tattttacaa gctaagcgtg ctcatgaatt ggaagctggt gagaaagcca ctcaagattt
    31501 taaatccgtg aaatcaacac ttcgcgcttt agaagaaatc gagtcaggta atgttgtaat
    31561 tcacccagat ccatcagcaa aacgtgcctc agttcgcgct cgtatcgaag ctgaacgcct
    31621 tgctaaagaa gaagaagaac gtaaaatcaa agagcaaatt gctaaagaaa aagaagacgg
    31681 cgaaaaaatt taagcaagtc aaaagtgaga ggttgaaaca ttctgtatca gcctcttttc
    31741 atattttaga gaattaggga gggtttatgg caagaaaatt agcacaggtt attgttgata
    31801 ttcctctgat gcagacagat aaaccatttt cgtatgccat tcctaaagat ttggaagatt
    31861 tggtacaagt aggagcacgc gttcatgttc cttttggaag agggaatcgt cttttgcaag
    31921 gctttgttgt cggtttcaga gatgatgatg aattagagac taaggatatt gcgggggtcc
    31981 tagattttga acctgtttta aatcaggaac aactagactt agctgatcag atgcgccata
    32041 ctgtcttttc ttataaaata tctattttga aatcaatgtt accaagtctt ttgaattcgc
    32101 aatatgataa actcttgtta gcaacggaca cattaccatc agaagataga gaagatctat
    32161 ttggtcataa aacggaaatc gtcttttcaa gtttgtcttc tcaagatgcc aaaaaagcag
    32221 gcagattaat tcaaaaaggt ttcatagaag ttcaatattt agccaaagat aagaaaacaa
    32281 taaaaacaga aaagatttac aaaatcaaca gaactctgtt agaaaagagc caaatagcag
    32341 caagagcgaa aaagagactg gagttgaaag aattcctgtt agaaaatcct caaccagggc
    32401 gcctcacagc tttaaacaaa caattttcat ctcctgtggt taattttttc agagaagaag
    32461 gaatcattga ggtcattgaa aaagaagcta gtagatctga taattatttt aaaggaatcc
    32521 taaaaacgga ttttttggat ttaaatcagg aacaagctaa agtagttaaa atagttgttg
    32581 accgaattgg taaggatcag aataaaccct tcttgttgga ggggattaca gggtctggta
    32641 agacagaggt atatcttcat atcattgata acgttttaaa gttgggaaaa acagcgattg
    32701 ttctcgttcc agaaatttct ttaactcccc agatgacgaa tcgttttatt tctcgttttg
    32761 gaaagcgagt tgctattatg cactcgggct tgtcagatgg tgaaaaattt gatgagtggc
    32821 gaaagataaa atcaggtcaa gctaaagttg tcgttggagc tcgttcagca atttttgctc
    32881 ctttggaaaa tatcggagcc atcattatag atgaagaaca tgaatcaact tataagcaag
    32941 aatctaaccc aaggtatcat gcgcgcgatg tggccttgct acgtgcagaa tatcataaag
    33001 ctgtgttact aatgggatca gcaactccaa gtatcgaaag ccgagctaga gctagtcggg
    33061 atgtttataa atttttagaa ttaaaacatc gtgctaatcc taaagcacgt attcctcagg
    33121 ttgaaattat tgattttaga aattttattg gtcaacaaga agttagtaat ttcacaccat
    33181 atttactaga taaaatccga gatagattag ataagaaaga gcaagtggtt cttatgctca
    33241 atcgtagagg ttattctagt tttattatgt gtcgagactg cggctacgtt gatcaatgtc
    33301 ctaattgtga catttctctg actttacata tggctacaaa gaccatgaat tgtcattatt
    33361 gtgggtttga aaagcctatt ccaagaacat gtccaaattg taatagtaaa agtatctctt
    33421 actatggtac tggaacccaa aaagcttatg aagaactttt gaaagttatc ccagacgcaa
    33481 agattttacg gatggatgtt gatacgacaa ggcaaaaagg cggtcatgag tctattttga
    33541 agcgttttgg aaatcatgaa gctgatattt tattgggaac acagatgatt gctaagggat
    33601 tggattttcc aaatgttact ttagttggag tactaaatgc agatacttct ctgaatttac
    33661 ctgattttcg atcatcagag cgaacttttc aactattaac tcaagtggct ggacgtgctg
    33721 ggcgtgctga aaaagaagga gaggttgtca ttcaaactta taatcctaat cattatgcta
    33781 ttcaattagc ccaaaagcaa gattttgaag ctttttatca atatgaaatg aatatccgtc
    33841 gacaacttgg ttatcctcct tattatttta cagttggttt aacattatcg cataaagatg
    33901 aagagtggtt aatacgaaaa tcttatgaag tattaagttt attgaagcaa ggctttagtg
    33961 ataaagtaaa gttgttaggt ccaacgccaa aaccaattgc aagaacacat aacctttatc
    34021 actaccaaat tattatcaag tatcgatttg aggacaacct tgaacttgtt ttgaatcgct
    34081 tattagacat gactcaagac aaggagaata gagatcttcg cttagcaata gaccacgaac
    34141 cgcaaaatat gatgtagaat agtgctatga tataattgag aaaagaaaga cgaggaaaaa
    34201 gcaatgacaa aattattatt catgggtacc cctgattttt cagcgacggt tttaaaaggt
    34261 attttagcag atggtaaata tgatgtttta gctgttgtga cgcaaccaga tcgtgctgta
    34321 ggtcgtaaaa aagaaattaa aatgacccct gtaaaagaag tggccttgga aaacaacatt
    34381 cctgtgtatc agccggaaaa actatctgga agtccagaat tagagcaact aatgacccta
    34441 ggtgcagatg gtattgtaac agctgctttt ggtcaattct taccaacaaa gcttttggaa
    34501 tcggtaggtt ttgcaattaa tgtacatgct tctctattgc caaaatatcg tggtggggca
    34561 ccaattcatt atgccattat taatggcgaa aaagaagctg gggttaccat catggaaatg
    34621 gttgcaaaga tggatgcagg ggatatggta tcaaaagctt ccgttgagat aactgatgaa
    34681 gacaatgtag ggacaatgtt tgatagactc gccgttgttg gtcgtgacct tttattagat
    34741 acgcttccag gttatctatc aggtgatatt aagccgatac cacaaaatga agaagaggta
    34801 agtttttctc ctaatattag tccagatgaa gagagaattg attggaataa atcatcgcgc
    34861 gacattttta accatgttcg agggatgtat ccatggccag ttgcacatac tcttttggaa
    34921 ggaaatcgtt ttaaacttta tgaagtaaca atgtcagaag gaaagggctc accgggtcaa
    34981 gtgatcgcta agacaaagaa ctcattgaca gtagcaactg gtgacggagc tattgaactc
    35041 aaatcagttc aaccagcagg gaaacctcgt atggatataa aagacttttt aaatggtgtt
    35101 ggtcgtaatt tagaaatagg agataaattt ggcgaatgat tggaaaaaga gtgcgcgtgg
    35161 tttagcttta atgactttag aggaagtttt tgataaagga gcttattcta atatcgcact
    35221 taataagagt ttaaaaaaat ctagactatc agataaagat cgtgccttag taacagaaat
    35281 tgtctatgga actgtggcta gaaaaataac gctagaatgg tatctatcac actttatagt
    35341 tgatcgtgat aagcttgaat tatgggtata tcatctcttg ctactgagtc tttatcaact
    35401 actctatctg gacaacatcc cagatcacgc tattgttaat gatgctgtca ctattgctaa
    35461 gaatcgaggc aataaaaagg gtgctgaaaa gttaattaat gccgttttac gaagagtaag
    35521 ttcggagacg ctccctgaaa ttgcatcaat aaaacgccaa aacaaacgtt actctgttgc
    35581 ttattccatg ccagtgtggc tcgtaaaaaa attaattgac caatatggtg agacaagagc
    35641 tttagctatt atggagagtt tatttgagcg aaataaagcc agtcttcgtg tgactgactt
    35701 atcgcaaaaa caaactatca aagaaacttt aaatgttaga gattcacata ttgcagagac
    35761 agctttagtt gcagactcag gaaattttgc atcgacatca tttttccaag atggcctcat
    35821 tactatccaa gatgaatcta gtcaattagt tgctccaaca ctcaaagtct caggtaatga
    35881 ccaagtattg gatgcatgta gtgcaccagg aggaaaaaca agtcatatcg cctcatattt
    35941 gactacaggg gcagtaactg ctttagattt gtatgatcat aaattggaat tggttatgga
    36001 aaatgcaaaa cgcctaggtc tgtcagataa gataaaaact aaaaagttag acgcaagtaa
    36061 agctcatgag tattttcctg aggatacttt tgacaaaatt ttagtagacg caccttgttc
    36121 aggtatcggg ctaattcgcc gtaaaccaga tattaagtac aataaagcta atcaagactt
    36181 tgaagccctc caagagattc aattgtctat cctatctagc gtttgtcaaa cgctacgtaa
    36241 aggtggtata ataacctata gcacttgtac catttttgag gaagaaaatt ttcaagttat
    36301 tgaaaaattc ttagcaaacc accccaattt tgagcaagta gaactgagtc atacacaaga
    36361 ggatattgta aagagaggct gtattagtat aagccctgaa caatatcata cagatggctt
    36421 ttttattgga caagtcaaac gtatcttgta gtgtctagga ggaaactgac atcgtcagaa
    36481 aaaaatatat ggaaatatct cttttaacag atattgggca acgtcgttcc aataatcaag
    36541 attttattaa tcaatttgaa aataaagccg gcgtcccact aatcatctta gcagatggta
    36601 tgggaggaca tcgtgcagga aatattgcta gcgagatgac tgtaactgat ctaggaagtg
    36661 attgggcgga gacagatttt tctgagttat cagaaattcg tgattggatg cttgtctcta
    36721 ttgaaactga gaatcgcaaa atatatgaat taggtcagtc agatgattac aaaggtatgg
    36781 gaactaccat tgaagcagtt gctattgtag gagataatat tattttcgca catgtaggag
    36841 attcacgtat cggtatcgtt cgtcaaggag agtatcattt attgacgagt gatcattctc
    36901 ttgttaatga attggtaaaa gctggccaat taacagaaga agaagctgct agtcatcctc
    36961 aaaaaaatat tattacgcaa tcaattggtc aagccaatcc agtcgaacct gatttaggag
    37021 ttcatctact tgaagaaggt gactaccttg tagttaatag tgatggacta actaatatgt
    37081 tgagtaatgc cgatattgct acagtactaa cacaagaaaa aaacttagat gataagaatc
    37141 aagatttaat cactctagca aaccatagag gaggtcttga taatatcact gtggcactag
    37201 tttacgttga aagtgaggcg gtttaatgat tcagattggc aaattatttg ctggtcgtta
    37261 tcggatcctt aaatcaatcg gacgaggtgg aatggcggat gtttatctag ctcgagatct
    37321 aattttagat aatgaagagg tcgcaataaa agtattgcga accaattatc aaacagatca
    37381 gattgcggtt gctcgcttcc agagagaagc acgagcaatg gctgagctga cacatcctaa
    37441 tattgtcgct attcgtgata taggtgaaga agacggacag cagttcttag taatggaata
    37501 tgttgatgga ttcgatttaa aaaagtatat tcaagacaat gcacccctat caaataatga
    37561 agttgttcga atcatgaatg aagtcctttc agccatgtca ctggcacatc agaaaggtat
    37621 cgtacaccgt gatttaaaac ctcaaaatat tttattaact aaaaaaggga ctgtaaaagt
    37681 tacagatttc ggtattgcgg tagcgtttgc tgaaactagt ttaacgcaaa cgaattctat
    37741 gttaggtagt gttcattatt tatcgccaga gcaggcgcgt ggttcaaaag cgactgttca
    37801 gagtgatatt tatgccatgg gaatcatgct ttttgaaatg ttaactggcc atattcctta
    37861 tgatggggat agtgcagtta ctattgccct acagcacttc caaaaaccac taccatcaat
    37921 ccttgcagaa aataaaagcg ttccacaagc tttggaaaac attgtcatta aagcaacagc
    37981 taaaaagcta acagatcgct ataagacaac ttatgagatg ggacgtgatt tatcaactgc
    38041 tctaagctcg acacgtcacc gtgaaccaaa acttgttttt aatgatactg agagtactaa
    38101 gacactacca aaagtaacat ctacagtatc atctttaacg acagaacaac tgttgcgtaa
    38161 tcaaaaacag gccaaaacaa cagaaaaaat aactcctgat tctgcgagta acgataaaac
    38221 gaagtctaaa aagaaggctt ctcatcgact tcttggtaca atcatgaaat tattctttgc
    38281 actctgtgtt gtaggaataa ttgtatttgc ttataaaatt ttagtaagtc ctacgactat
    38341 tcgtgttcca gatgttagca acaaaacagt agctcaagca aaaatgacat tggaaaattc
    38401 tggattgaaa gtaggagcta ttcgtaatat tgagagtgat agtgtttcag aaggccttgt
    38461 cgtaaaaaca gaccctgctg caggacgttc tcgtagagaa ggggctaaag ttaatcttta
    38521 tattgccact ccaaacaaga gtttcactct aggtaattat aaggaacata attacaaaga
    38581 tatcctcaaa gatcttcaag gcaaaggtgt taaaaaatct ttaatcaagg ttaggcgtaa
    38641 aattaataat gattatacta ctggtacaat cttagcacag tcgcttccag aaggaacatc
    38701 ttttaatcct gatggtaata agaaattaac acttacggtt gctgttaatg atccaatgat
    38761 tatgcctgat gtaacaggta tgacagttgg agaagtaata gaaaccttga cagacctcgg
    38821 attagatgca gataatcttg tcttctatca aatgcaaaat ggcgtttatc aagcagtagt
    38881 aacacctcca tcttcaagta agatagcaag tcaggatcct tattacggag gagaagttgg
    38941 attaaggaga ggagataaag ttaagctgta tcttcttggt tctaaaacaa caaataactc
    39001 atcatctacg ccaatagatt cttcggcttc aagctcaaca ggaacaacta cctcggattc
    39061 tgtaagctca tcaacggatg ctagcacttc tgatagtagt agcacttcta caagtagttc
    39121 aacactacca agtgatagta caactaatac aggaacagct aacaatccat taactcaata
    39181 aaattaaaaa gaagatgtta aagtcttctt tttaatttgc tatggtagag accatgccta
    39241 gaaaggttct gccaatatgc caaatttttc tctcaataat ggtgcaaagt atagggcttt
    39301 ttgataaaat acgtcatata gagtaatgga aggattatta tgaaaaaatt tcagtttttt
    39361 ctacttgttg aagccgttgt cttagttatg ggattaatga aaattttatc agatgactgg
    39421 acaagtttta tttttattct cgctttgatt cttttagcct taagatttta taacaacgat
    39481 agtcgtcata attttctgtt aacgacaagt ttgttattac tatttttaat ctttatgctt
    39541 aacccttaca ttattgcagc cgttgttttt gcagttttgt atgttctaat caatcatttc
    39601 tcgcaagtta agaaaaaaaa tcgttatgct ttgattcaat ttaagaacca tcaattagat
    39661 gttaagacaa cacgaaacca atggttaggt actgatcagc acgaaagtga cttttatgct
    39721 tttgaagata ttaatattat aagaatctct gggactgata caattgattt aacaaatgtt
    39781 attgtatctg ggcaggataa tgttattata attcaaaaag tttttggtga tactaaagta
    39841 ttggttcctt tagacgttgc cgtaaaggct gatattagtt ctgtttacgg tagtgtacag
    39901 tactttgatt ttgaggagta tgatctaaga aatgaatcta taaaattgtc acaagaggaa
    39961 gaatattacc ttttgaaacg tgtcaaatta gttgtaaaca ctatcgctgg gaaagttgag
    40021 gttagtcgta aatgaaaaag caccactact ttttagcttt cttctatgga agtgtcatta
    40081 tatttgccat ttgttttgtc attattgata gtctgggtgt taatttggtg cacctctatc
    40141 aaacgtctcg tttgtggctg attgaacaat tgattttctc tattttcttt ctatctttag
    40201 cggtaactat ccttttattg ctaacttggt ttttattaga tgataatagt aaacgtcaaa
    40261 ttaaccataa tttacgtcgc attttaaata accaatcaat aaatgttacc gatgatggta
    40321 ctgaaatatc gaccaatatt caacggctct ctaaaaaaat gaatttaatg acagcaagtc
    40381 tacaatctaa ggaaaatagt cgtattttaa aaagccaaga aattgtgaaa caagaacgta
    40441 agcgtattgc acgtgattta cacgacacag ttagtcagga tttgttcgca gcttccatgg
    40501 ttttgtcagg gattgcacag aatgtttctc agctagacgt tgatcaggtt ggctcgcaat
    40561 tattagcagt agaggaaatg cttcagcatg cacaaaatga tttgagaatt ctgctacttc
    40621 atttgaggcc agttgaatta gaaaataaga ccctatctga aggattcaga atgatattga
    40681 aagaattgac cgataagagc gacattgaag ttgtttatca tgaatcaatt ctaaccttac
    40741 ccaaaaagat tgaagacaat atttttagaa ttggtcaaga atttatcagt aatactttaa
    40801 agcattcaca agccagtcgt ctcgaagttt accttaatca aacagagaat gaattgcagt
    40861 taaaaatgat tgataatggt attggatttg atatggattc tgtctatgat ttaagttatg
    40921 ggttaaaaaa tattgaagat cgagtagaag atttagcagg aaatttacag cttctcagtc
    40981 agcctggtaa aggagttgct atggatataa gattaccttt agtaaatcag agtgaggata
    41041 aaaatggata aaataaaaat tgtacttgtt gatgaccatg agatggttcg tctcggttta
    41101 aaaagttttt taaacttaca agctgatgtt gaagtaatag gtgaggcctc aaatggttta
    41161 gaaggcataa aaaaagcact ggagttacgt ccagacgttg ttgttatgga cttagtaatg
    41221 cctgaaatgg acggtgtaga agcaacctta gcattattga aggattggcc agaagctgct
    41281 attttagtct taacatctta cttggacaat gaaaaaattt accctgttat tgaagcaggt
    41341 gccaaaggct atatgttaaa gacctcaagt gcagcagaga ttcttaatgc cattcgtaaa
    41401 gttgcaaggg gagaacaagc tattgaaaat gaagtagata agaaaattaa ggctcatgat
    41461 aaatgtccag ctttacatga gggcttaaca gcaagggaac gtgatatcct taatttatta
    41521 gccaaaggtt atgacaatca aaggattgct gatgaattat ttatctcgtt aaagactgtt
    41581 aaaactcatg tttctaatat tttgggaaaa ctaaatgtag cagatcgaac tcaagcggtt
    41641 gtctatgctt tccaacacca tttggtacca caagatgatg aataagtgat ataatagagg
    41701 cactatgtta atgaaagtgg ttagtcatgg acgcaaaaac taaatataaa gcaaaaaaaa
    41761 taaaggcagt gttttttgat attgatgaca cgcttcgtgt taaagataca ggttatatgc
    41821 caccatcaat cttaaaagtt tttaaagctt tgaaagataa aggaattgtt gtaggtattg
    41881 cttcaggaag ggcacgatat ggcgttccaa aagaagttca agatttaaat gccgattatt
    41941 gtgtaaaatt aaatggagct tatgttaagg acaaggataa gaatatcatc ttccatcgtc
    42001 ctattcctgc tgaatatgta gagcaatata agaaatgggc agacactgtt ggtattaagt
    42061 acggtttagc tggtcgtcat gaggcagttt tatcagatcg tgatgatttg gtaaatgatg
    42121 cgattgatat tgtgtattct gatctcgaag ttaatccaga tttcaataaa gaacatgata
    42181 tttatcagat gtggacgttt gaagataaag gagatagcct tcatctacct gaaccgttag
    42241 atgaacatct tcgcttgata cgttggcatg atcattcttc agatgttgtg ttaaaaggaa
    42301 catcgaaggc tttaggtgtt tctaaagttg ttgaacattt gggcttaaag ccagagaata
    42361 tcttggtttt cggagatgag ctcaatgatt tagaactctt tgattatgct ggactagctg
    42421 tggcgatggg agtgtcacac ccagaagctc aaaaaaaagc agattttatc acaaaaaaag
    42481 tagaagaaga tggcatcttg tatgccctgg aggaactagg tttgatagaa aaagaattaa
    42541 catttccaca agtagatatt gaaaatacag aaggtcctgt ggctgtcatt aaaacaaatc
    42601 acggggatat gactgtaaaa ctcttcccgg atcatgctcc taaaacggta gcaaacttta
    42661 ttggtttagc taaacaaggt tattatgatg gtattatctt ccatcgtatt attccagatt
    42721 ttatgattca gggtggtgat ccaactggta caggtatggg tggtgaatct atctatgggg
    42781 aaagttttga agacgaattt tccgaggaat tatataatgt tcgcggagct ctttcaatgg
    42841 ctaatgctgg accaaatacg aatggcagcc aatttttcat cgtccaaaat acaaaaattc
    42901 cttatgcgaa aaaggaatta gagcgaggtg gttggccaac accaattgct gagttgtatg
    42961 ctggacaagg tggtacaccg catctagatc gtcgccacag tgtatttgga caattagtaa
    43021 accaaagttc ttttgaggta cttgatgaga ttgcggcagt agagactggt tctcaagaca
    43081 aaccattgga agatgttgtt attttaacga ttgaggttaa agaataaaat gaagattggc
    43141 gataagttaa agggaacagt cactggcatt aggccttatg gagcctttgt ttctcttgag
    43201 gatggtagga ctggacttat ccatatttct gaaattaaaa caggttacat tgataatatt
    43261 tatgatgttc ttagtgtagg tgatgaagtc tatgttcagg ttatagatgt agatgaattt
    43321 acacaaaaag caagtttatc gcttcggacc cttgaagaag aaagacatca tattcagcat
    43381 cgtcatcgat tttctaacaa tcgtcttaag ataggtttca aaccacttga ggaaaattta
    43441 cctagttggg ttgaggaagg tctagcctac ttgaataata aataaacaaa aaacctagat
    43501 ttgattaatg acaagtctag gttttaaata tttaaaagta gcagtgaata ttatacttgc
    43561 taaaaatagc ttgataatcg agtagatcct ctgggtgtag tgcagcgatc tcttccattt
    43621 catattgacg attaagtaat tgatatttgt tttgtccaaa ttggtggaaa ggtaagagtt
    43681 gcacttgacg gatatctagg cgatcaaata aacaagcaaa ttcttcagca tcttctaatg
    43741 aatcgttaaa attaggtatg acaggaatac gtagcacaat agttttaccg ttggcaaaag
    43801 cataatgaat gtttttgatg attgaagcat tcttaaccat cgtcttttct tggtgtttga
    43861 ggctattgta atgttttaaa tcggtgtaaa taaaatcaac gtattggata agatctatga
    43921 attgttcatg tctagtataa gcagttgttt caatagccgt gtggattccc agtgatttag
    43981 cacgttttaa aattgccttg gcaaattcaa attgtgcaaa tatttcaccg ccagatagcg
    44041 ttatgccccc accagattct tcgtaaaaat caatatcttt taaaacctct tcaataatat
    44101 catcaaccga tttttcttcg cccacgatga cgctttcatt agtaatggca tctctcattg
    44161 tttctggaac cattttttgc gactcgggat tagcacacca aggacatcta agaggacaac
    44221 cctttaagaa aacggttgtt cgaatacctg gaccatcgtg aatactaaaa tgttgaatgt
    44281 tgaatacaat acctttttca tttgtcatat tattttcctc aatttcattt taagtatacc
    44341 ttttatacgt aacaaagtca attacgaatg aaagtttatt gaacttgttt aataaataaa
    44401 aaatgataaa atagaaagga ggtgtttcat gaatagatta gaaaatatta ttagtttagt
    44461 atctcagtac caaaaaatag acgttaatac actctcggag ttgttacaag tttctaaagt
    44521 aacgatacgt aaagatttag ataaactaga aggcaaaggg cttttgcata gagagcacgg
    44581 ttatgcagtt ttaaacagtg gggatgatct taatgttagg ttatctttta accataaaac
    44641 taaaaaagaa attgctgcct tagctgcgaa tatggtgtct gacaatgaca ctatcttaat
    44701 agaatcaggc tctacttgtg ctcttttagc ggaaaatatc tgtcagacaa agcgaaatgt
    44761 cactattttg accaactctt gctttattgc taactattta cgagaatatg attcatgcca
    44821 aattgtctta cttggtggtg agtatcaatc aagttcacag gttacggtag gtcctctctt
    44881 gaaaaagatg atttcacttt ttcatgtctc gttagctttt gttgggactg atggttttga
    44941 tccaaaacta ggatttatgg gtagagattt aatgcgttct gaagttgcac aagagatggc
    45001 gaatgctgcc gatgaagtca ttattttaac agattctagt aagtttaatc agactgcatt
    45061 ggtggaacag cttccgttgt caactgtatc acaagttatt acagataaac atccaaattc
    45121 ggaaattgct aatttgttcc aagaagcaga gattaccatt caatctttat tacaagtgcc
    45181 ataaaagagg ctatttttct tacattttgc acatttgtgg cgataataga taaagtgtca
    45241 tgtcaaattg cacagatgta acctttttgt taaactgaag caaatgctga aaggttatga
    45301 gtatgaaatt agagcgtcaa aaattgttag ctaaagtagc ttacctttac tatatggaag
    45361 ggaaaagcca atctgagatt gccaatgaat taggaattta tcgtacaaca ataagtcgta
    45421 tgttagccaa agcgagagaa gaaggtcttg ttagaatcga aatttcagat tttaatccag
    45481 aaatttttca gttagaatcc tattttaaaa gtaaatatca ccttaaagat attgagattg
    45541 tttcttcgcg aaaagatagc gatacttcag agatagagaa ggatttagca catgtagctg
    45601 cggctatgat tcgaaaaaaa attaaagaga atgacaaggt ggggattgct tgggggagga
    45661 cacttagtaa agtagttgaa gcaatgcgtc ctcatccggt ttctcaagtt tcttttgttc
    45721 ctttagctgg aggtccaagt catattaatg cacgctatca cgttaatact ctggtttacg
    45781 aaatgtcaag gcgtttccaa ggaagttgta ctttcattaa tgcaactcta gttcaagaaa
    45841 acgctaattt agcgaaagga atattaactt caaaatattt tgaggggcta atggataatt
    45901 gggaaaaact tgatgtggca attgtgggcg ttggtggtaa acctaaaagc aatgaacaac
    45961 agtggcttga tcttttaaat caagatgatt ttcaatgctt ggatgaagaa gcagctgtag
    46021 gtgaaattac ttgtcgtttt tttaatcatt caggtgatcc tgtgaatcaa catttagcta
    46081 aaaggacaat aggaattacc ttagaacaac tacagaaagt tcctaataga atagcggttg
    46141 ctcatggcaa ttataaggct gcggcactct tagccgtttt aaaaaagggg tatattaatc
    46201 atttagttac tgatttttcc acagccttaa atattttaag attagataaa gacacctttg
    46261 tggatactat atatcaaaag agctaggaat aaatcctagc tcttttgata atgtttctat
    46321 taccaagtga attgttatag aataagcaca aaggtgcgct attgtcataa agggcacaag
    46381 gtctattgac gacgattaag ggacatgtta catttgtttt gcgaaagaaa attagaacct
    46441 tttgtaagaa agtttaaact tataaaaaat cattaaagcg gaggtgctta tggaaatcat
    46501 agtcgcagac caaattatta tggggctcat tttgaatgct ggggatgcta aacaacatat
    46561 ctatcaagcc ttaaaacttg caaaagaagg aaattttgca gaaagtaaaa ttgaaataga
    46621 actagcagat agtgcactcc tagaagctca taatcttcaa acacaattcc ttgcacaaga
    46681 agcaggaggg acaaggacag atatctcagc actctttatt cattctcagg atcatttgat
    46741 gacaagtatt actgagatta acttgattaa agaaattatt gatttacgcc aggaattaca
    46801 tattcaaaaa taagtaaagg ggatcaaata tgataaaaat tggtttattc tgtgcagcag
    46861 gtttttcaac aggtatgtta gtaaacaata tgaaaatcgc agcggataaa gaagggatag
    46921 aggcacatat tgaagcttat tcacaaggaa aaattgcaga ttatgctaaa gatttagatg
    46981 ttgcattact tggtccacaa gttagctata ctctagataa atcaaaatca atctgtgatg
    47041 aatacggtgt accgatagca gttattccaa tggcagatta tggtatgttg gatggtgtaa
    47101 aagttttgaa acttgctttg agtttattgg aaacaagaaa agaggcttga tatgtcaaag
    47161 tttgatagtc agaaaataat tactccgatt atgaagtttg tcaatatgcg agggattatt
    47221 gcactcaaag atggcatgct agcaattcta ccactaacag ttgttgggag tctcttttta
    47281 atattagggc agcttccatt taaaggtctt aatcaagcca tagctaatgt ttttggacca
    47341 gaatggacag aaccatttat gcaagtttat tcaggaactt ttgcgattat gggcttgatt
    47401 tcctgttttg caattgctta tgcttatgct aaaaatagta gtgtagaacc gttacctgct
    47461 ggcgttttat cactttcgtc tttctttatt ttaatgaaat catcttatat tcctgtaaaa
    47521 ggagaagcaa tagcggatgc tatttctaag gtttggtttg gcggtcaggg gattattgga
    47581 gcaattatta ttgggttagt agttggtgct atctatacat ggtttatcca acatcatatt
    47641 gttataaaaa tgccagagca agtaccacag gcaatagcaa aacaatttga agctatgatt
    47701 ccagcttttg ttatctttct tttatcgatg attgtttatt tgattgctaa ggtaacaact
    47761 ggaggtacct ttattgagat gatttatgat atcattcaag taccgttgca aggcttaaca
    47821 ggctcacttt atggagcaat tgggattgct ttctttattt cattcttatg gtggtttggt
    47881 gtccatggcc aatctgtggt gaatggtatt gtgactgctt tgttactatc caatttagat
    47941 gccaataagt ctttattagc agcaaatcgc ttaacattag ataatggcgc tcacattgta
    48001 acacagcagt ttttggatag tttcttgatt ttatcgggct caggaataac ttttggacta
    48061 gttattgcga tgctttttgc agcaaaatca aaacaataca aggcacttgg gaaagtggcc
    48121 gcttttcccg cgattttcaa tgttaacgag ccaatcgtct ttggctttcc aatcgtaatg
    48181 aatcctgtga tgtttctgcc ttttatttta gtaccagttt tggcagcttt aattgtttat
    48241 ggagctattg cggttggttt tatgcaacca ttctcaggtg ttactttacc atggagtact
    48301 ccagcaatta tttctggatt catggtaggt ggttggcaag gtgcactcgt ccaaatagtc
    48361 attttagcta tctcaactgc tgtttacttc ccattcttta aaatccaaga taatattact
    48421 tacaaaaatg aatgtgaaat ggaaagggta tagctagcag acaagaggtt gaggtgtgta
    48481 atgcacctta acctttttct aaaaatgcac atttgtgcaa ctttagatgt agcattctat
    48541 tctttatttt cttcttgtta ttttaggatt taggtgctat tatatttacg aagaaaaact
    48601 ttttagtttc gaaagaaaca agcggaggct gctatgacca aacaattact cactcaaaaa
    48661 tatacaaatg atgttcaaca aaattctcaa aagcattttg gctatttgac agagcgcatg
    48721 tattcttatc gtgataaggt tcttgataag aaaccattca tagatgcaga acgtgctatc
    48781 ttggtaactg aagcctatca aaaacatcag gaaaaaccta atgttttaaa gagagcttac
    48841 atgttgcaaa atattttgga aaaaatgaca atctatattg acgatgagac aatgattgtc
    48901 ggtaaccaag cttcatctga taaagatgct cctatttttc cagaatacac tctagaattt
    48961 gttgtaaatg aactagacct ttttgaaaaa cgagatggtg acgtctttta cattacagaa
    49021 gaaaccaaag aacaaattag aaatattgca cctttctggg aaaataataa tttacgggct
    49081 cgtgcgggcg ttatgcttcc agaagaagtt caagtttata tggaaacagg cttctttggg
    49141 atggaaggta agatgaattc tggggatgct cacttagctg ttaactatca gaagttacta
    49201 gaagaagggc ttattggctt tgaaaaaaaa gcaagaaaag ctaaagctga cttagattta
    49261 acaaagcctg agagtattga caaatatcat ttctatgatt ctattttaat tacaatagaa
    49321 gctgttaaaa cttatgcaga gcgttttgct atcctagcaa aaaaacaagc taaaacagca
    49381 aatgctaaga gacgtcagga attgttagat atcgctagta tttgtgaacg agtgccatat
    49441 tacccagcta aaacgtttgc agaagctgtt caatcggttt ggtttattca gtgtattctt
    49501 caaatagaat caaacggtca ttctctatct tatggacgct ttgatcaata catgtatcca
    49561 tatgttaaag ctgacttaga agctggacgt gagactgagg attcgattgt tgagcgccta
    49621 actaacctat ggattaaaac aataactatt aataaggttc gtagccaagc tcatacgttt
    49681 tcgtctgctg gtagtcctct ttaccaaaat gtaacgattg gaggacaaac ccgacataag
    49741 gaagatgctg ttaatccttt atcatttcta gtactaaaat cagtcgctca gacacactta
    49801 ccacagccta atctaacggt tcgttatcat gctaacctag acaagtcatt catgaatgaa
    49861 gctattgaag ttatgaaatt aggctttggt atgccagctt ttaataatga tgaaattatt
    49921 attccatcat ttattaaaaa aggagtttcc gaagaagatg cctatgatta ttcagcgatt
    49981 ggttgtgttg aaacagcagt tcctgggaaa tggggttatc gttgtaccgg catgagctat
    50041 atcaacttcc ctaaagtgtt attaattaca atgaataatg gcattgaccc ggcttctggg
    50101 aagagatttg cgccttctta tgggcatttc acccagatga cctcatataa agaacttaaa
    50161 gaagcttggg acaaaacact tcgttactta actcgaatga gcgttattgt tgagaatgct
    50221 attgatatta gtctcgaaag agaagtacct gatattcttt gttcagcttt aactgataat
    50281 tgtattggac gaggtaagca cttaaaagaa ggtggtgcag tatatgacta catatctggt
    50341 ctacaagttg gaatcgctaa tctttcagac tcacttgctg cgcttaaaaa attagtattt
    50401 gaagaaaaac gcctaaccac attagaagta tggcaagcat tacagagtga ttacgcaggt
    50461 ccgcgtggtg aagaaattcg tcaaatgttg atcaatgaag caccaaaata tggaaatgat
    50521 gatgattacg cggatagttt agtacgtgag tgttatgatg tttatgttga agaaattgct
    50581 aaatatccca acacacgcta tggacgtgga ccaatcggtg gtattcgcta ttcaggaacg
    50641 tcctctattt cagcaaatgt aggccaagga cgaggtactt tagcaactcc agatggtagg
    50701 catgcaggaa ctccattagc tgaaggttgt tcaccatctc ataatatgga caaaaaggga
    50761 cctacctctg ttttgaaatc agtttcgaag ttaccaactg atgagattgt cggcggtgta
    50821 ctccttaacc aaaaagtcaa ccctcaaacc ttggcaaaag aagaagataa acaaaaatta
    50881 attgccttac ttagaacatt cttcaatcgc cttcatggtt accatatcca gtataatgtt
    50941 gtctcacgtg agactttgat tgatgctcag aagcaccctg aaaaacaccg cgatttgatt
    51001 gtgcgtgtag caggttattc tgcattcttt aatgtcctat ctaaggcaac tcaagatgat
    51061 attattgcac gtacagaaca tgctctttag gagattatta tggaattttt acttgacact
    51121 ttaaatttag aagctataaa aaaatggcat catatactgc cactagcagg tgtaacttca
    51181 aatcccacca ttgctaagaa agaaggagat atacattttt tccaacgcat tcgtgatgtt
    51241 cgagaaatta ttggacgtga agcaagtttg catgttcaag tagtggccaa agattatcaa
    51301 gggatactgg atgatgctgc taaaatacgc caagaaacgg atgatgacat ttatatcaaa
    51361 gtcccagtta cgccagatgg attagcagca attaaaacgt taaaagcaga aggttataat
    51421 attacagcca cagcaatata caccagtatg caaggtcttt tggctatatc agctggagca
    51481 gactacctag caccttactt taaccgaatg gagaaccttg atattgatgc gacacaagtt
    51541 attaaggaat tagcgcaagc aatagagcgg acaggttctt ctagcaaaat tttagcggct
    51601 agttttaaaa acgcttcaca agtaacgaag gccttatcac agggtgctca atcaatcacc
    51661 gcgggaccag atatttttga atcagtcttt gcgatgccat ctattgcaaa agctgttaac
    51721 gactttgcag atgattggaa agccagtcaa cactcagaac atatttaaca gagaacgcca
    51781 acttttgagg ctgtatcttt aacataaaat tttttaaaga agaaagaggt tgagaatgaa
    51841 agttttcgca agtccatcgc gctatataca aggaaaggat gctttattcc aaagtattga
    51901 acatatcaaa tctttaggtc agactccact tatcctttgt gatgatgttg tttataatat
    51961 cgttggagaa agttttttat cttatctaca agacgatttg ttaccacatc gtgtctcctt
    52021 taacggagaa gcatctgata atgaaatcaa tcgtgtcgtt gctgttgcga aagaaaagaa
    52081 tagtgacctt attattggac ttggtggtgg aaaaactatt gactctgcaa aggcaattgc
    52141 agataaagtt aatttaccag tggtcattgc accaacagta gcttcaacag atgccccaac
    52201 atcagcgtta tcagtaatct atacagatga aggagctttt gaaaaatata ttttttattc
    52261 aaagaatcca gacttagttt tggtcgatac tcaagtaatt gctcaagcgc caaaacgttt
    52321 actagcttct gggattgcag atggcttagc tacttgggtt gaagcgagag cagtcttaca
    52381 aaaaaatgga atagcgatgg ctggcggtag acagacttta gcgggcgtgg caattgccca
    52441 agcatgtgag cgtactttat ttaatgatag tttacaagct ttagcggctt gtgatgccaa
    52501 agtggttaca aaagctttag aaaatgttat tgaagctaat accttactta gtggtttagg
    52561 ttttgaaagt gcaggattag ctgcagctca tgctattcat aatggtttta ctgccttgag
    52621 tggagatatt catcacttaa cccatggtga aaaagttgcc tatggaacgt tgacgcaact
    52681 tttcctcgaa aatagaccaa aggaagaaat tgatcgctac attaatcttt accaagcgat
    52741 tggaatgcca actacacttg cagaactcca tttgggtgat gcaacatatg aagaactttt
    52801 aaaagttggt cagcaagcaa caattgaagg tgaaacaata catgaaatgc cattcaaaat
    52861 tagtgcagag gatgtcgctg cagccctgtt agcagttgac cgctatgttt caaatcatca
    52921 ataaaaacaa tagaaccaag tgaaattgct tggttctatt tgtcgtttta atattttaac
    52981 taaatagggc ttactagtat ttaaagagat ataaactaaa aaagatcaga caacatatct
    53041 aatctttttt agttatcgtt tactgaagat taaagttatt caaaatcata caggctagtt
    53101 gataagtaac gttcgccatt atcagggaga agagctaaaa cctttttccc ttttcctact
    53161 tgtttggcca cttcaatagc agcataaata gcagctgaag cagaaattcc tgcgaggaaa
    53221 ccttctagtc cgccgatgat acgtcctgtt tcaatagcat cgtcagatgt aacgcgaatg
    53281 atatgatcat aagaatctgt agccaatgtt tctgggatga aacctgcaga aatcccttgt
    53341 attttatgag gcccaggttg ctcaccagaa aggattgcag attcatcagc ttctacagca
    53401 taaatagcga catcaggatt gacttttttt aatgcacgag agacaccagt aattgtccca
    53461 ccagttccca cacctgcgat aaaagcatct aaaccttttt caccaaaagt ctcgataatt
    53521 tccctaccag tcgttttttc gtggatttct ggattggctt gattattaaa ttgcaagggt
    53581 aaccaagcat tttgctctac agaaatttct tttgccttag caatagctcc cttcatccct
    53641 tcactaccag gtgttagtac taattgagca ccataggctt gaatgatttt acgacgttca
    53701 attgacatgg tttctggcat aacaataata acattatacc ctttagcttt tcctacccaa
    53761 gccaaaccga tacctgtatt accagatgta ggttctacga ttgtatctcc ggctttaaga
    53821 atcccttttt gctctgcatc ttcaatcata gctagagcaa tacgatcttt gacagatgaa
    53881 cctgggttaa aggattctaa cttaacgtac acatctgcag ctccttctgg aacaatatga
    53941 tgtaattgaa taatgggtgt atttccgatt aagtctgtaa tagaattata aatttttgac
    54001 ataagctata cctcttcatg atattggtta agaatattat aaaactcaca ttgattaaag
    54061 taaaatatca attctttatc aagtgatagc tattatttat cggtttcatc cttaaaggga
    54121 atttcaacaa tttgactccc ttcttcagtt aacaaagctt ttccttggta gaactctgtc
    54181 aaattagtta ttgtgtgttc aaattcctta gggtcaacgt atattttaca ggttacagct
    54241 tctaaaaatt ctgtttcaaa ctcctgaagg tggtgctctt ttaggaagtt gtcaaaagtt
    54301 tgatattgag gataggttaa ttgaatacga ataccaattt gttctttaac ttctactacc
    54361 tcgatctctt tgatggcatt agctacgcta ccagagtagg cacgaataag cccccctgtg
    54421 cctaatttga tacctccaaa ataccgtgta acgacggcaa caacatttgt caggccttgc
    54481 ttttcaagta cggttagcat agggatacct gctgtaccac taggttctcc atcatcacta
    54541 gatcgtttta tatccccttt ttcgccaata accatagctg aacacgaatg attggctttg
    54601 tagtgcgctt tcttgatttg cgtaatataa ttacgcccct cctcttcgga ctctactcgc
    54661 tttagatgac aaataaatag cgatttttta atttcttctt cgacaattcc gtctgatttt
    54721 atcgttttat agttcataga gaaattttac aaaaattctt caaatttagc aaaaaaatcc
    54781 gaatgaaata atatggaaaa ttatttaggt agactttgga caaaagcaca attatctgag
    54841 caacttagaa aaattgcaat taacttgcca tcttttataa agaaaggaag cgactatatt
    54901 tgtactcgat gctctagttc tgttgccaaa aattgccaat taccaactgg aaattattat
    54961 tgtagagaat gtatagtttt tggaagagtt actagtaatg aaaatttata ttatttccca
    55021 caaaaaacat tttctaaaac gaattcttta aaatggaaag ggggactgac accttatcaa
    55081 aatgaggttt cagaagagtt gttgaaaggt atttcaagta aggaaaatct tcttgttcat
    55141 gctgtgacag gggctggtaa aacagaaatg atataccact ctatcgccaa agtaattgat
    55201 actggaggaa gcgtttgtat tgccagtcct agaattgatg tttgtttaga attgcataag
    55261 cgactttcaa acgattttag atgtgccata acattaatgc atggagaatc tccttcttat
    55321 cagagatccc ctttaactat tgccacaaca catcaattgt taaaatttta tcatgctttt
    55381 gatttgctaa ttgttgatga agttgatgct tttccatatg tcgacaaccc gatactatac
    55441 catggtgtaa aacaggcctt aaaggaaaac ggaacaagta tttttctgac tgcaacttca
    55501 acgactgagt tagaaagaaa agttgcaaga aaagaactaa aaaaattaca tctagctagg
    55561 agatttcatg ccaatccgtt agtgataccc gagatggttt gggtaagtgg tatccagaaa
    55621 agcttgcaga cacaaaaact acctcccaaa ttatatcagc tgataaataa acagcgacaa
    55681 acgagatatc ctttgttatt atttttcccc catatttcag aaggtcaggt attcaccgaa
    55741 atgttaagac aagcctttcc tatggaaaaa attggttttg tttcaagtaa gagcacatct
    55801 aggttaaagt tagtgcaaga ttttcgagat aataaattgt ctatcctagt gtcaactacc
    55861 attctagaga gaggagtaac tttccctagc gtcgacgttt ttgttataca agcaaatcat
    55921 catttgttta cgaaaagttc cttggtacaa atttcaggaa gagttggaag agcattagaa
    55981 aggcccgaag ggctccttta cttccttcat gatgggaaaa gtaaatcgat gcaccaagct
    56041 ataaaggaga ttaaaaacat gaatcatata ggaggatttt aatgacttgt ttgttatgtc
    56101 atgaaataga tttatcgcaa ttaacttttg tggaacttat gcttctaaaa ccaaagcaaa
    56161 acgtaatttg tcaaacgtgc aagggtagtt ttgaagcact tagtagggag atgggttgcc
    56221 aaacatgttg caagcaaatt ccccaaaaac aatgtcaaga ttgtatttat tggggtaaaa
    56281 aaggtataga ggtaaatcat ttttcccttt atagatacaa tgaagcaatg aaaaaatatt
    56341 tttctctttt taaatttcaa ggggattatt tgttgaaaga tgtttttaca aaagaaataa
    56401 aagctgctct aaaaaggtat aagggctaca ctatagtgcc agttcccttg agtcatgaag
    56461 gataccaaaa taggcaattt aatcaagtga ttgcttttct acaatcggca aatatacctt
    56521 ataaaaatat tctttctaaa aaagatagag gtaagcaatc ggctaataat aaagaagaaa
    56581 gactcaaaca agttcagcaa tttacattaa aaaatgaggc tgagttaagg gataaccttt
    56641 taattgttga tgatatttat acaacaggcg caacaatagc acaaatcagg aaactattag
    56701 aagaaaaagg tataaaaaat ataaaaagtt tttcattagc acgctaaaat cttgcaaatt
    56761 ttcatgaaag cggttataat aagaatatag aaagcgttaa caacgcaaga aagaggtact
    56821 catatgatta aatacagtat tcgtggtgaa aacatcgaag taacagaagc tattcgtgag
    56881 tatgttgaga ccaaactctc taaagttgaa aaatacttta atgaagaaca agaattagat
    56941 actagagtca acctcaaagt atacagagag aagaccgcaa aagttgaagt aacaatctta
    57001 atagattcta ttactttacg agcagaagat gtttcacaag atatgtatgg gtcaattgac
    57061 ttagttgttg ataaaattga gcgtcaaatt cgtaaaaata aaactaaaat tgcgaaaaaa
    57121 tatcgtgaaa aaattccagc aagtcaagtt ttcacaacag aatttgaggc tgagccagat
    57181 gaagaagcag taagccaaag aattgtacgt actaagaatg ttaatctaaa accaatggat
    57241 gttgaggaag cattgttgca aatggaactt ttaggtcatg atttctttat ctatacagat
    57301 gcagaagata atacgacaaa tgtcctttac aagcgtgaag atggcgaact aggtttaatt
    57361 gaagctaaag gaaattaact ttgtaacata atttaaaaaa tctcagagtt ttttctgaga
    57421 tttttttaaa acctattgac aacaaataaa aaattatagt atactaaatg agttcttaag
    57481 cgaacctaat attgtattat cattttataa acaactt
//
LOCUS       DATRTN010000006        55494 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.006, whole genome shotgun sequence.
ACCESSION   DATRTN010000006 DATRTN010000000
VERSION     DATRTN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 55494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55494
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.006"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..446
                     /locus_tag="VBE22_000358"
     CDS             <1..446
                     /locus_tag="VBE22_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEO7201836.1"
                     /translation="FSKPVLQIIVNMYDLGDTLTAWLLNLAQPVTFLTIFLGIGILYF
                     ILPNARIRKVRYVIPGTLFSTFVIGFFSNLISQYVLNRVEKMVDIKTFGSVVIFILML
                     WFIFLAHIMILGAILNASVQEIATGKIESRRGDIMSLIQKSKEEK"
     gene            complement(463..918)
                     /locus_tag="VBE22_000359"
     CDS             complement(463..918)
                     /locus_tag="VBE22_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEO7201837.1"
                     /translation="MVKLKSLLKKSIQNEVSLYLLFGLLTSLLYLVIRQGIFNFSQNA
                     PFSAIVANIIAILFAFFTNDRFVFKQTKIGQFQRLQTFVIARLGTLGLDLILAVIFVD
                     QFPSIIGQFVQHNLNKINTIESLVSQILIILLNYILSKFVIFKDKKRQL"
     regulatory      989..1102
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            1100..1609
                     /locus_tag="VBE22_000360"
     CDS             1100..1609
                     /locus_tag="VBE22_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEO7201838.1"
                     /translation="MNTFTTRDYAHMAIVTAIYIVLTITPPFNAIAYGAYQFRVSEML
                     NFLAFYHRKYLFAVTLGCMISNLYSFGMIDVFVGGGSTLLFVYLGTILFKQYQKDYLF
                     NGLINKAFFFFSFFFAASMITVAVELKIVAGLPLLLTWLTTAVGELASLLVGAVLVDK
                     LSRHVDFTK"
     gene            1749..3707
                     /gene="ligA"
                     /locus_tag="VBE22_000361"
     CDS             1749..3707
                     /gene="ligA"
                     /locus_tag="VBE22_000361"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEO7201839.1"
                     /translation="MENRMNELVSLLNQYAKEYYTQDNPTVSDSQYDQLYRELVELEE
                     QHPENILPNSPTHRVGGLVLEGFEKYQHEYPLYSLQDAFSKEELIAFDKRVKAEFPTA
                     SYMAELKIDGLSVSLTYVNGILQVGATRGDGNIGENITENLKRVHDIPLHLDQSLDIT
                     VRGECYLPKESFEAINIEKRANGEQEFANPRNAAAGTLRQLNTGIVAKRKLATFLYQE
                     ASPTQKETQDDVLKELESYGFSVNHHRLISSSMEKIWDFIQTIEKDRISLPYDIDGIV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPNQIKERLAHFASRDAMNITGFGPSLVEKLFD
                     AHLIADVADIYRLSIEDLLTLDGIKEKSATKIYHAIQSSKENSAEKLLFGLGIRHVGS
                     KASRLLLEEFGNLRQLSQASQESIASIDGLGGVIAKSLHTFFEKEEVDKLLEELTSYN
                     VNFNYLGKRVSTDAQLSGLTVVLTGKLEKMTRNEAKEKLQNLGAKVTGSVSKKTDLIV
                     AGSDAGSKLTKAQDLGITIQDEDWLLNL"
     gene            3719..4738
                     /locus_tag="VBE22_000362"
     CDS             3719..4738
                     /locus_tag="VBE22_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO7201840.1"
                     /translation="MKKQKKARLIYNPTSGQEIMKKNVAEVLDILEGFGYETSAFQTT
                     PTKNSARDEAARAAQAGFDLIVAAGGDGTINEVVNGIAPLKRRPKMAIIPTGTTNDFA
                     RALKIPRGNPIEATKLIGKNQIVKMDIGQAQEDNYFINIAAAGSLTELTYSVPSQLKT
                     TFGYLAYLAKGVELLPRVRKVPVKITHDKGEFIGDASMIFVAITNSVGGFEQIAPDAK
                     LDDGKFTLILVKTANLIEIMHLIRLVLAGGKHINDKRVEYIKTSYLTIEPLSDERMMI
                     NLDGEYGGDAPITLANLKNHIRFFANTDEISDDALVLDKDELAIEAIAQKFANEVDDL
                     EGLGD"
     gene            4742..7042
                     /gene="pulA"
                     /locus_tag="VBE22_000363"
     CDS             4742..7042
                     /gene="pulA"
                     /locus_tag="VBE22_000363"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEO7201841.1"
                     /translation="MDNTVIVHYHSRKKNYFNLSLWQWRDGSEGQDAHFSRFDSFGAV
                     AILKYPLPYFLSHYYVIVKDKNWHHKTIDYRIDCTKGGARKEVWIVDGDDNLYYSRQA
                     AVSSHHYSRRQPHAYDMAINSRAFDKKWGFDGWLGFEYSETKTAFRLWAPTAERVELI
                     LYHSTDETASVSKVLSMKRGTAVNYKNHKENTHGVWFTELEGNYNYQAYTYRVYYRRR
                     TFKITRDPYSIATTANGKRSIVIAPEALTPEGFKISHGKEAKWRLENPNQAVIYEMHV
                     RDFSISETSGVKTDYQGKFKGLHQKDTVNQHGDKTTFDYVQDLGVNYIQLQPIFDHHQ
                     TFDDDGHYAYNWGYDPENYNVPEASFSSNPHEPATRILELKSAIQAYHDAGIGVIMDV
                     VYNHTFSSTDSAFQLTVPDYYYRMNHNGTFQNGSGCGNETASEKEMCRKYILDSVLYW
                     VKEYNIDGFRFDLMGLHDVETMNIIRNELNKIDPRILVYGEGWDMGAGLTPQNKAKKD
                     NAYQMPGIGFFNDDVRDAVKGAEIYGEFKKGLVSGNSTEDIVAKGILGSDELVSYIDP
                     SQVLNYVEAHDNYNLNDLLWELHPNDNEKQHIYRVEVASAMNLLMQGMAFMQLGQEFL
                     RTKCYPTGDKGQLTQADKERAMNSYNAPDQVNQVNWDNVTFHKSTINFIRKIITLKTN
                     SPYFSYSSFEEIRKHVFVESAQYHSGFISFTVEEHKKIKIIFNIKGKRLQNPFIGDII
                     ISNHEEGKAIDSLDDELSVMIFDITK"
     gene            7248..9116
                     /gene="glgB"
                     /locus_tag="VBE22_000364"
     CDS             7248..9116
                     /gene="glgB"
                     /locus_tag="VBE22_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEO7201842.1"
                     /translation="MSNKDELYTFGIGENFHLQNYLGVHSENESFCFRVWAPNAENVQ
                     VIGDFTDWRNRPLQMNKNQAGVWEANSLDAREGDLYKYLVTRKGGQVVEKIDPMAVYM
                     ERRPGTASVIKVLRNKKWEDGLWMGRRKRLGFQKRPINIYEVHAGSWKKDDFGHPMTF
                     SQLKDYLIPYLVEMNYTHVEFMPLMAHPLDMSWGYQLMGYFAFEHTYGTPEEFQDFVE
                     ACHKNNIGVLVDWVPGHFIQNDDALAYFDGTATYEYQNHDRAHNYRWGALNFDLGKNQ
                     VQSFLISSALFWIEHYHIDGIRVDAVSNMLYLDYDEGPWEANQFGDNRNLEGYYFLRK
                     LNKVIKERHPNVMMIAEESTASTPITKDLESGGLGFDFKWNMGWMNDILRFYEEDPLY
                     RQYDFNLVTFSFMYIFNENFVLAFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYAYQ
                     MCHPGKKLLFMGSEFGQFLEWKYNDQLEWENLNDDMNQKMQRYTKQLNQFYKDHKCLW
                     RIDDSFDGIEIIDADNKSETVLSFIRKDDKGDLLLCVFNMTPVERPNFTIGVPQAGIY
                     EEVLNTEMEEFGGVWKNHNPVTKTQVATWKDYDHTLSFTLPALGASVWRIKRRLRK"
     gene            9158..10297
                     /locus_tag="VBE22_000365"
     CDS             9158..10297
                     /locus_tag="VBE22_000365"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEO7201843.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPDVQFGGRYRIIDFALS
                     NCANSGINNVGVITQYQPLELNTHIGNGSSWGLDGIDSGVTVLQPYSATEGNRWFQGT
                     SHAIYQNIDYIDRINPEYVLILSGDHIYKMDYDDMLQTHKDNLASLTVAVLDVPLKEA
                     SRFGIMNTDSNDRIVEFEEKPEHPKSTKASMGIYIFDWKRLRTVLIDGEKNGIDMSDF
                     GKNVIPAYLESGERVYTYNFDGYWKDVGTIESLWEANMEYIGEDNKLHSRDRSWKIYS
                     KNLIAPPNFMTEDANVKDSLVVDGCFVAGNVEHSILSTNVQVKPNAIIKDSFVMSGAT
                     IGEGAKINRAIIGEDAVIGDGVVIDGSKEVEVIGYKEVVGVPNED"
     gene            10287..11420
                     /gene="glgD"
                     /locus_tag="VBE22_000366"
     CDS             10287..11420
                     /gene="glgD"
                     /locus_tag="VBE22_000366"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEO7201844.1"
                     /translation="MKIDKYTAILGNTVSYPDMHSLTDNRPLASLPFDGKYRLIDFQL
                     SSLANAGVRSIYGIFRGNNVRSVFDHIRSGREWGLGTLLSHYSLGFYDTDDDIRTVNG
                     DYYDQILTYLRRSGSDQTIYMSCDMLCNIDLSQVIHLHNANGTQATVVYKKMPRSAIA
                     EANQVLTIDESDHVVSLGKAVDNDDNQKMSAEIYVINTPWLIEKMEEEAQNNEPRKLR
                     FLLRDLIVESNALAFEYTGYLSNISSIKSYYDANMDMLTPNKFYSLFFSNQKVYTKVK
                     NEEATYFDKQSNVSNSQLASGSIIKGYLDHSIVSRNCLLENGTRVVNSIIFPKVKIGE
                     GATIENTIIDKCVKVAPGVTLKGSLDKPLVIPKFSEINEDIIQ"
     gene            11417..12847
                     /gene="glgA"
                     /locus_tag="VBE22_000367"
     CDS             11417..12847
                     /gene="glgA"
                     /locus_tag="VBE22_000367"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEO7201845.1"
                     /translation="MKIMFVAAEGAPFAKTGGLGDVIGALPKSLSKKGHDVVVVMPYY
                     DMVDQKFGDQIENLMYFYTDVGWRHQYVGVKRLSQDNVTFYFIDNQYYFYRGHVYGDW
                     DDGERFAYFQLAALELMEKINFIPDVLHVHDYHTAMIPFLLKEKYHWIQAYNNIRTVF
                     TIHNIEFQGQFGPEMLGDLFGVGAERYEDGTLRWNNCLNWMKAAILYSDRVTTVSPSY
                     ANEIKTPEFGKGLDQIMRMEAGKLSGIVNGIDSDLLNPETDAFLPYHFSKSNLEGKIK
                     NKLALQENLGLPQDKNVPLIGIVSRLTDQKGFDIIASELDNMLQQDIQMVILGTGYHH
                     FEETFSYFASRYPEKLSANITFDLRLAQQIYAASDIFMMPSAFEPCGLSQMMAMRYGS
                     LPLVHEVGGLKDTVVAFNQFDGSGTGFSFNHFSGYWLMQTLKLALEVYNDYPEAWKKL
                     QWQAMSKDFSWDTACVAYEQLYQQLQ"
     gene            13179..13379
                     /locus_tag="VBE22_000368"
     CDS             13179..13379
                     /locus_tag="VBE22_000368"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEO7201846.1"
                     /translation="MNLAILALGFAVMGVSIGEGILVANIAKSAARQPEMFSKLQTLM
                     FTGVAFIEGTFFVLFAFTFLVR"
     gene            13412..14128
                     /gene="atpB"
                     /locus_tag="VBE22_000369"
     CDS             13412..14128
                     /gene="atpB"
                     /locus_tag="VBE22_000369"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO7201847.1"
                     /translation="MESTSNPTVSFLGIDFDLTILAMSLLTITIIFILVFWASRKMTI
                     KPKGKQNVLEYVYELVNNTISQNLGHYTKNYSLLMFILFSFVFVANNLGLMTSLKTHE
                     HNFWTSPTANFGVDITLSLLVAFICHIEGIRKKGIGGYLKGFLSPTPAMLPMNLLEEV
                     TNVASLALRLFGNIFSGEVVTGLLLQLAVLSPFTGPLAFALNIVWTAFSMFIGFIQAY
                     VFIILSSSYIGHKVHGDEEE"
     gene            14146..14643
                     /gene="atpF"
                     /locus_tag="VBE22_000370"
     CDS             14146..14643
                     /gene="atpF"
                     /locus_tag="VBE22_000370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEO7201848.1"
                     /translation="MSILINSTTIGDIIIVSGSVLLLFILIKTFAWKQITGIFEAREQ
                     KIANDIDTAEQARQQAEAFATKREEELSNAKTEANQIIDNAKETGLAKGDQIISEAKT
                     EADRLKEKAHQDIAQNKAEALADVKGEVADLTVLLAEKIMVSNLDKEAQSNLIDSYIK
                     KLGDA"
     gene            14643..15179
                     /locus_tag="VBE22_000371"
     CDS             14643..15179
                     /locus_tag="VBE22_000371"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEO7201849.1"
                     /translation="MNKKTQALIEQYSKSLVEVAIEHKIVEKIQQEVAALIDIFETSE
                     LEGVLSSLAVSHDEKQHFVKTLQTSCSTYLVNFLEVIVQNEREALLYPILKSVDQELI
                     KVNGQYPIQITTAVALSPEQKERLFDIAKTKLALPNGQLVEHIDPSIVGGFVVNANNK
                     VIDASVRNQLHQFKMKLK"
     gene            15195..16700
                     /locus_tag="VBE22_000372"
     CDS             15195..16700
                     /locus_tag="VBE22_000372"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEO7201850.1"
                     /translation="MAINAQEISALIKKQIEDFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFSNGAYGMAQNLESNDVGIIILGDFSEIREGDVVKRTGKIMEVPVGEA
                     MIGRVVNPLGQPVDGLGEIETTATRPVETPAPGVMQRKSVFEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRKYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYNGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDALGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGAAQIKAMKRVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     NDILAFEEALYDYFDAHYDNLFETIRTTKDLPEEAELDAAIQAFKDQSQFK"
     gene            16716..17597
                     /locus_tag="VBE22_000373"
     CDS             16716..17597
                     /locus_tag="VBE22_000373"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEO7201851.1"
                     /translation="MAGSLSEIKDKILSTEKTSKITSAMQMVSSAKLVKSEQAARDFQ
                     VYASKIRQITTNLLKSDLVSGSDNPMLASRPVKKTGYIVITSDKGLVGGYNSKILKAM
                     MDTITDYHTENDDYAIISIGSVGSDFFKARGMNVSFELRGLEDQPSFDQVGKIIAQAV
                     EMYKNELFDELYVCYNHHVNSLTSQVRMQQMLPIKELDADEASEDRVITGFELEPNRE
                     VILEQLLPQYTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQA
                     AITQEITEIVAGANALE"
     gene            17671..19077
                     /gene="atpD"
                     /locus_tag="VBE22_000374"
     CDS             17671..19077
                     /gene="atpD"
                     /locus_tag="VBE22_000374"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEO7201852.1"
                     /translation="MSSGKIAQVVGPVVDVVFASGDKLPEINNALIVYKNGDKSQKVV
                     LEVALELGDGLVRTIAMESTDGLTRGLEVLDTGRAISVPVGKDTLGRVFNVLGDAIDL
                     EEPFAEDAERQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALTPEIVGDEHYEVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAETFTGQPGSYVPVEETVRGFKEILDGKHDQIPEDAF
                     RMVGGIEDVIAKAEKMNY"
     gene            19090..19503
                     /locus_tag="VBE22_000375"
     CDS             19090..19503
                     /locus_tag="VBE22_000375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEO7201853.1"
                     /translation="MAQLTVQVVTPDGIRYDHHASLITVRTPDGEMGILPGHINLIAP
                     LIVHQMKINRSHQEGVDWVAVNGGIIEVNEDQVTIVADSAERARDIDLNRAERAKERA
                     ERALEKAQTTQNIDEMRRAEVALRRAINRISVGKK"
     gene            19568..19798
                     /locus_tag="VBE22_000376"
     CDS             19568..19798
                     /locus_tag="VBE22_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEO7201854.1"
                     /translation="MILNIVTIVSNFIFIALFYQLFVDLFDWSKMIKMSPQNISKLKV
                     FILLISIVGGYVVSHFLLEVIQLCQSIFWALQ"
     gene            19861..21132
                     /gene="murA"
                     /locus_tag="VBE22_000377"
     CDS             19861..21132
                     /gene="murA"
                     /locus_tag="VBE22_000377"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO7201855.1"
                     /translation="MDKIIVEGGQTRLQGQVVIEGAKNAVLPLLAATILPSQGKTLLT
                     NVPILSDVFTMNNVVRGLDIQVDFNCDKKEILVDASGDILDVAPYKFVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPIDLHLKGLEAMGATITQNGGDITAQAEKLKGANI
                     YMDFPSVGATQNLMMAATLASGTTTIENAAREPEIVDLAQLLNKMGAKVKGAGTETLT
                     IIGVDALHGTEHDVVQDRIEAGTFMVAAAMTSGNVLVKDAIWEHNRPLISKLMEMGVE
                     VSEEEDGIRVKADTKKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMIETVFEN
                     RFQHLEEMRRMGLQTEILRDTAMIHGGRALQGAPVMSTDLRASAALILAGMVAQGQTV
                     VGQLTHLDRGYYQFHEKLAALGANIKRVSEA"
     gene            21134..21325
                     /locus_tag="VBE22_000378"
     CDS             21134..21325
                     /locus_tag="VBE22_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO7201856.1"
                     /translation="MASSGWKYVLKQIGLIVLVILLALLFLAVGLMLGYSVFGDGEHA
                     YSILSLDKWQNIIGKFLGK"
     gene            21400..22257
                     /locus_tag="VBE22_000379"
     CDS             21400..22257
                     /locus_tag="VBE22_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7201857.1"
                     /translation="MTKKQKQITTSIVTLLFSLLAIGLGESKSLPNDHILKQVTDLII
                     SNESGKNTSLKNVSGTPSRELSESVLTSNVKKQLGTNIAWNQSGAFIINQNKTDLNAK
                     VSSAPYAINEIKKVNNQIVPTKANALLTKATRQYRNREETGNGRTYWKPAGWHQINGL
                     KGSYNHAVDRGHLIGYALVGSLRGFDASTSNPKNIATQAAWANQANSNQSTGQNYYET
                     LVRKALDRHKTIRYRVTLIYDRDNLLSSGSHIEAKSSDGSLEFNVFIPNVQSGLLFDY
                     ATGKVKQTK"
     gene            22548..23588
                     /gene="pheS"
                     /locus_tag="VBE22_000380"
     CDS             22548..23588
                     /gene="pheS"
                     /locus_tag="VBE22_000380"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEO7201858.1"
                     /translation="MDLQKQLEELKISTQEKLKEMTGNHTKELQDLRVQVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDILTKAFEEQAKVVEAAKIQAQLESESVDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQVVGGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTMDQHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQI
                     EGLVVGENISMGDLKGTLQLISQKMFGAERKIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKQTGWIEILGAGMVHPSVLEMSGIDSEKYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDVRFTDQF"
     gene            23671..24192
                     /locus_tag="VBE22_000381"
     CDS             23671..24192
                     /locus_tag="VBE22_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7201859.1"
                     /translation="MSQITFKKVGLDNVNILQNLAIETFRQTFSHDNSEEQLQAFFNE
                     SYTLPVLKSEITHAESDTYFVYLDTDLVGYLKVNWGSQQTEKDLDKAFEIQRIYLLDA
                     YQGKGIGKATFEFALDLAYKSGLDWAWLGVWEFNHKAQAFYAKYGFEKFSEHQFSVGD
                     KVDTDWLLRKSLH"
     gene            24246..26651
                     /locus_tag="VBE22_000382"
     CDS             24246..26651
                     /locus_tag="VBE22_000382"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEO7201860.1"
                     /translation="MLVSYKWLKELVDVDVTTAELAEKMSTTGIEVEGVETPAEGLSK
                     LVVGHIVSCEDVPDTHLHLCQVDTGDDELRQVVCGAPNVKTGINVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSESIIPKEFSDGIQILPEGAIPGDSIFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYGKKVHFEEKNLIEEAERAADKISVVIESDK
                     VLSYSARIVKNVTVAPSPQWLQNKLMNAGIRPINNVVDVTNYVLLTYGQPMHAFDFDK
                     FDGTTIVARNAENGEKLITLDGEERDLIADDLVIAVNDQPVALAGVMGGQSTEIGSSS
                     KTVVLEAAVFNGTSIRKTSGRLNLRSESSSRFEKGINYDTVSEAMDFAAAMLQELAGG
                     QVLSGQVTEGVLPTEPVEVSTTLGYVNTRLGTELTYTDIEEVFEKLGFAISGSEVKFT
                     VLVPRRRWDIAIQADLVEEIARIYGYEKLPTTLPEAGATAGELTSMQRLRRRVRTVAE
                     GAGLSEIITYALTTPEKAVQFSTQATNITELMWPMTVDRSALRQNVVSGMLDTIAYNV
                     ARKNSNLAVYEIGKVFEQTGNPKEDLPTEVETFTFALTGLVEEKDFQTKAKPVDFFYA
                     KGIVEALFIKLKLDVTFVAQKGLASMHPGRTATILLDGKEIGFVGQVHPQTAKQYDIP
                     ETYVAEINLSTIESQMNQALIFEDITKYPSVSRDIALLLAESVSHHDIVSAIETSGVK
                     RLTAIKLFDVYAGNNIAEGYKSMAYSLTFQNPNDNLTDEEVAKYMEKITKSLVEKVNA
                     EIR"
     gene            complement(26720..27622)
                     /locus_tag="VBE22_000383"
     CDS             complement(26720..27622)
                     /locus_tag="VBE22_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEO7201861.1"
                     /translation="MKIDDLRKSDNVEDRRSSSGGSFSSGGSGLPILQLLLMRGSWKT
                     KLVVLIILLLLGGGGLTSIFNDSSSPSSYQSQNVSRSVDNSATREQIDFVNKVLGSTE
                     DFWSQEFQTQGFGNYKEPKLVLYTNSIQTGCGIGESASGPFYCSADKKIYLDISFYNE
                     LSHKYGATGDFAMAYVIAHEVGHHIQTELGIMDKYNTMRHGLTKKEANALNVRLELQA
                     DYYAGVWAHYIRGKNLLEQGDFEEAMNAAHAVGDDTLQKETYGKLVPDNFTHGTAEQR
                     QRWFNKGFQYGDIQHGDTFSVEHL"
     gene            27800..31033
                     /gene="rexB"
                     /locus_tag="VBE22_000384"
     CDS             27800..31033
                     /gene="rexB"
                     /locus_tag="VBE22_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEO7201862.1"
                     /translation="MKLLYTDINHDMTEILVNQAAHAAEAGWRIFYIAPNSLSFEKER
                     AVLENLPQEASFAITITRFAQLARYFTLNQPNQKESLNDIGLAMIFYRALASFEDGQL
                     KVFGRLKQDASFISQLVDLYKELQTANLSILDLKYLHSPEKFEDLLAIFLVVSDLLRE
                     GEYDNQSKIAFFTEQVRSGQLDVDLKNTILIVDGFTRFSAEEEALIKSLSSRCQEIII
                     GAYASQKAYKANFTNGNIYSAGVDFLRYLATTFQTKPEFILSKWESKSGFEMISKNIE
                     GKHDFTNSSHILDDTAKDCITIWECINQKDEVEHVARAIRQKLYQGYRYKDILVLLGD
                     VDSYKLQLSKIFEQYDIPYYFGKAETMAAHPLVHFMDSLSRIKRYRFRAEDVLNLFKT
                     GIYGEISQDDLDYFEAYISYADIKGPKKFFTDFVVGAKKFDLGRLNTIRQSLLAPLES
                     FVKTKKQDGIKTLNQFMFFLTQVGLSDNLSRLVGQMSENEQEKHQEVWKTFTDILEQF
                     QTIFGQEKLNLDEFLSLLNSGMMQAEYRMVPATVDVVTVKSYDLVEPHSNQFVYALGM
                     TQSHFPKIAQNKSLISDIERQLINDANDTDGHFDIMTRENLKKNHFAALSLFNAAKQA
                     LVLTIPQLLNESEDQMSPYLIELRDIGVPFNHKGRQSLKEEADNIGNYKALLSRVVDL
                     YRSAIDKEMTKEKQTFWSVAVRYLRRQLTSKGIEIPIITDSLDTVTVSSDVMTRRFPE
                     DDPLKLSSSALTTFYNNQYKYFLQYVLGLEEQDSIHPDMRHHGTYLHRVFEILMKNQG
                     IESFEEKLNSAINKTNQEDVFKSLYSEDAESRYSLEILEDIARATATILRQDSQMTVE
                     SEEERFELMIDNTIKINGIIDRIDRLSDGSLGVVDYKSSAQKFDIQKFYNGLSPQLVT
                     YIDAISRDKEVEQKPPIFGAMYLHMQEPKQDLSKIKNLDDLVTKNHQALTYKGLFSEA
                     EKEFLANGKYHLKDSLYSEAEIAILQAHNQLLYKKASETIKSGKFLINPYTEDAKTVD
                     GDQFKSITGFEADRHMARARALYKLPAKEKRQGFLTLMQQEEENDDL"
     gene            31023..34646
                     /gene="addA"
                     /locus_tag="VBE22_000385"
     CDS             31023..34646
                     /gene="addA"
                     /locus_tag="VBE22_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEO7201863.1"
                     /translation="MTFKPFLNPEDIAVIQTEEKNSDKKQKRTPEQIEAIYTFGNNVL
                     VSASAGSGKTFVMVERILDKLLRGVPIDSLFISTFTVKAAGELKERLEKKINESLKSA
                     ESDDLKQFLTQQLVGIQTADIGTMDAFTQKIVNQYGYTLGISPIFRILQDKNEQDVIK
                     NEVYADLFSDYMTGKNAASFIKLVKNFSGNRKDSKAFREMVYKVYAFSQSTDNPKRWM
                     QTVFLKGAQTYTDFEAIPDQEVSSLLNVMQTTANQLRDLTDQEDYKQLTAKGVPTANY
                     KKHLKIIENLVHWSQDFNLLYGKKGLANLARDITNVIPSGNDVTVAGVKYPIFKQLHN
                     RIVGLKHLEVIFKYQGESLFLLELLQSFVLDFSEQYLQEKIQENAFEFSDIAHFAIQI
                     LEENHDIRQLYQDKYHEVMVDEYQDNNHTQERMLELLSNGHNRFMVGDIKQSIYRFRQ
                     ADPQIFNDKYKAYQDNPSQGKLIILKENFRSQSEVLDSTNSVFTHLMDEEVGDILYDE
                     SHQLKAGSPRQQERHPNNKTQVLLLDTDEDDINDSDSQQYDISPAEAKLVAKEIIRLH
                     KEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRS
                     IDNPLNDYALVALLRSPMFGFNEDDLTRIAIQDVKMAFYHKVKLSYHKEGHHSDLITP
                     ELSSKIDHFMKTFQTWRDFAKWHSLYDLIWKIYNDRFYYDYVGALPKAEQRQANLYAL
                     ALRANQFEKTGFKGLSRFIRMIDKVLENENDLADVEVALPQNAVNLMTIHKSKGLEFK
                     YVFILNIDKKFSMVDITSPLILSRNQGIGIKYVADMRHELEEELLPAVKVSMETLPYQ
                     LNKRELRLATLSEQMRLLYVAMTRAEKKLYLVGKASQTKWADHYDLVSENNHLPLASR
                     ETFVTFQDWLLAVHETYKKQELFYDINFVSLEELTDHHIGMVNPSLPFNPDNKAENRQ
                     SEDIVRAISVLESVEQINQTYKAAIELPTVRTPSQVKKFYEPILDIEGVDVMETITKT
                     SVDFKLPDFSTSKKQDPAALGSAVHELMQRIEMSSHVKMEDIQKALTEVNAETSVKAA
                     IQIEKINYFFQETSLGKYIQEEVEHLHREAPFAMLKEDPESGEKFVVRGIIDGYLLLE
                     NRIILFDYKTDKFVNPLELKERYQGQMALYAEALKKSYEIEKIDKYLILLGGKQLEVV
                     KMD"
     gene            34659..35576
                     /locus_tag="VBE22_000386"
     CDS             34659..35576
                     /locus_tag="VBE22_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO7201864.1"
                     /translation="MIVEQKFGNGFTWINIEAEQLRTETSEIQAKYLDSEIITYALDD
                     YERAFMECSHIKGKEVLTIIFNTIDLKQKESYYETVPMTFCLSHDRLITVTRSRNSYM
                     LELLQKYLDRNPDVSPKKFLFAALTLITKQYFNVVSKIDREKDILNRQLREQTTNKRL
                     LAMSDLETGSVYLLTAANQNALVLEQLDVHPSQRFNSEVEKEQLSDALIEAHQLVSMT
                     QLNSQVLSQLSSTFNNVLNNNLNENLTGLNIISINLAIIAAITGFFGMNIPLPLTESR
                     SSWLIVIATSVLLWVIIAQILKRVFNLRK"
     gene            complement(35607..36983)
                     /gene="mnmE"
                     /locus_tag="VBE22_000387"
     CDS             complement(35607..36983)
                     /gene="mnmE"
                     /locus_tag="VBE22_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEO7201865.1"
                     /translation="MSITKEFDTIAAISTPLGEGAIGIVRISGTDALKIASKIYRGKD
                     LSAIQSHTLNYGHIVDPDKNEILDEVMLGVMLAPKTFTREDVIEINTHGGIAVTNEIL
                     QLILRHGARMAEPGEFTKRAFLNGRVDLTQAEAVMDLIRAKTDKAMDIAVKQLDGSLK
                     TLINNTRQEILNTLAQVEVNIDYPEYDDVEEMTTTLMREKTQEFQALMENLLRTARRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREEKAIVTDIEGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRDTDDIVEKIGVERSKKALEEADLVLLVLNSSEPLTLQDRSLLELSKESNRI
                     VLLNKTDLPQKIEVNELPENVIPISVLENENIDKIEERINDIFFDNAGMVEHDATYLS
                     NARHISLIEKAVDSLKAVNEGLELGMPVDLLQVDMTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     gene            37111..39021
                     /locus_tag="VBE22_000388"
     CDS             37111..39021
                     /locus_tag="VBE22_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO7201866.1"
                     /translation="MIILQGNKIERSFSGDVLFDNINIQVDQRDRIALVGRNGAGKST
                     LLKILVGEETPTKGEINKKRDLSLSYLAQDSRFQSENTIFQEMLQVFDSLREVEKRLR
                     ELELQMGQVSGSDLEQLMKTYDILSEEFREKGGFTYESDIKAILNGFKFNSDMWEMPI
                     SELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIDTIAWLENYLVNYQGALIIVSHD
                     RYFLDKVATVTYDLTTHSLDRYVGNYSKFMDLKAEKIATEEKNFEKQQKEIAKLEDFV
                     QRNIVRASTTKRAQARRKQLEKMERLDKPNVEQKSANMTFHAGKVSGNVVLTLENAAI
                     GYEGVSLSELIDLDVKKFDAIAIVGPNGIGKSTLIKSLVGQIPFIKGEAKLGANVETG
                     YYDQSQSNLTKTNTVLDELWNAFSTTPEVEIRNRLGAFLFSGDDVKKLVSMLSGGERA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIEFDGTLLFVSHDRYFINRVAT
                     KVLEISDKGSTLYLGDYDYYLTKKAELEELARLNEEEVSASKTEIDVTSDYETQKANQ
                     KEFRKITRRIAEIEARLEVLENNENNINGLMLETNDIGKLSDLQKELESIQEEQLLLM
                     EEWENLNMRLDL"
     gene            39170..40138
                     /locus_tag="VBE22_000389"
     CDS             39170..40138
                     /locus_tag="VBE22_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEO7201867.1"
                     /translation="MVTLSKEQHLDMFLKMQRIRDVDMKFNKLVRRGFVQGMTHFSVG
                     EEAASVGAIQDLTDSDIIFSNHRGHGQTIAKGIDIGGMFAELAGKATGTSKGRGGSMH
                     LANLEKGNYGTNGIVGGGYALAVGAALTQQYEGTDNIVIAFSGDSATNEGSFHESVNL
                     AAVWNLPVIFFIINNRYGISTDITYSTKIPHLYMRADAYGIPGHYVEDGNDLMAVYEK
                     MHEVINYVRSGNGPAIVEVESYRWFGHSTADAGVYRTKEEVDSWKAKDPVKRYRAYLI
                     ENEIATEEELAAIEAQVIKEVEEGVKFAEESPFPDMSVAFEDVFVD"
     gene            40213..41211
                     /locus_tag="VBE22_000390"
     CDS             40213..41211
                     /locus_tag="VBE22_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEO7201868.1"
                     /translation="MSETKVMALREAINVAMSEEMRKDEKVFLMGEDVGVYGGDFGTS
                     VGMLEEFGAKRVRDTPISEAAIAGSAIGAAQTGLRPIVDLTFMDFVTIAMDAIVNQGA
                     KTNYMFGGGLSTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGLKVVAPGTVNESKAL
                     LKSSILDNNPVIFLEPKALYGKKEEVNMDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVMQAAEEVAEEGINVEVVDPRTLIPLDKELIIDSVKKTGKLILVNDAYKTGGFTGEI
                     ATMVAESEAFDYLDHPIVRLASEDVPVPYSRVLEQGILPDVAKIKDAIYIVVNKGK"
     gene            41338..42726
                     /locus_tag="VBE22_000391"
     CDS             41338..42726
                     /locus_tag="VBE22_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEO7201869.1"
                     /translation="MAVEIIMPKLGVDMQEGEILEWKKQVGDVVNEGDVLLEIMSDKT
                     NMEIEAEDSGVLLKITHGNGDVVPVTETIGYIGAEGEEVTEASSSENTSVEENATQVT
                     SEPEKVEETSEPSVPAATSGEKVRATPAARKLAREMSIDLALVSGTGANGRVHREDVE
                     SFKGAQPRITPLARRIAEDQGVDIAEITGSGIRGKIVKNDVLAAMSPQAAEAPVETKA
                     TPTTEEKQLPEGVEVIKMSAMRKAISKGMTNSYLTAPSFTLNYDIDMTEMMALRKKLI
                     DPIMAKTGLKVSFTDLIGMAVVKTLMKPEHRYLNASLINDAQEIELHNFVNIGIAVGL
                     DDGLIVPVVHNADQMSLSDFVIASKDVIKKTQEGKLKSAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRIVDGMNGAKFMVDL
                     KNLMENPFGLLI"
     gene            42786..44543
                     /gene="lpdA"
                     /locus_tag="VBE22_000392"
     CDS             42786..44543
                     /gene="lpdA"
                     /locus_tag="VBE22_000392"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEO7201870.1"
                     /translation="MAFDVIMPKLGVDMQEGEILEWKKNEGDTVNEGDVLLEIMSDKT
                     NMEIEAEDTGVLLKIVHQAGDVVPVTEVIAYIGEEGEEVGTSSPSADATITAEDGQSV
                     SGPAAPSQETVAAATPKEELAADEYDIVVVGGGPAGYYAAIRGAQLGGKIAIVEKTEF
                     GGTCLNVGCIPTKTYLKNAEILDGLKVAAGRGINLASTNYAIDMDKTVAFKNSVVKTL
                     TGGVRGLLKANKVEIFNGLGQVNPDKSVVIGDKVIKGRNVVLATGSKVSRINIPGIES
                     PLVLTSDDILDLREIPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAMDKE
                     VSLELQKILAKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVADKALLSIGRVPQMN
                     GLENLEPELEMERGRIKVNAYQETSIPGIYAPGDVNGTRMLAHAAYRMGEVAAENALG
                     GNKRKAHLDFTPAAVYTHPEVAMVGMTEEQAREQYGDILVGKNSFTGNGRAIASNEAH
                     GFVKVIAEPKYKEILGVHIIGPAAAELINEASTIMENELTVYDVAQSIHGHPTFSEVM
                     YEAFLDVLGEAIHNPPKRK"
     gene            44641..45630
                     /locus_tag="VBE22_000393"
     CDS             44641..45630
                     /locus_tag="VBE22_000393"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO7201871.1"
                     /translation="MKYIVNTSNDPAYNVALEAYAFQKLTDIDEIFILWINEPAIIIG
                     RHQNTIQEINKEFIDKNGIHVVRRLSGGGAVYHDLNNLNYTIISNNTQEGAFDFQTFS
                     KPVIDTLAKLGVKAEFTGRNDLEINGQKFAGNAQAYYKGRMMHHGCLLFDVDMSVLGQ
                     ALKVSKDKIESKGIKSVRARVTNIVDHLSDKITVQEFSDAILAQMKEEYPEMDEYVLS
                     DAELSEIQAMRDNQFATWDWTYGKAPEYTIERGVRYPAGKITTYANVENSTIKSVKIF
                     GDFFGVKPVDDIEKMLEGVRYDYKDVLAALKTVDTSQYFSRMTPEEITKAIVD"
     gene            complement(45737..46522)
                     /locus_tag="VBE22_000394"
     CDS             complement(45737..46522)
                     /locus_tag="VBE22_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEO7201872.1"
                     /translation="MTYTSLKSPTTKDYKYTLNVAHLYGNLLNTYGDNGNILMMKYVG
                     EKLGCQMTFDIVSLEDRFDPNYYQMAFFGGGQDYEQAIVARDLPSKKEDINKFIQNNG
                     VVLAICGGFQLLGQYYIQANGERIEGIGVMGHYTLNQNNNRYIGDIKIHNDEFNETYY
                     GFENHQGRTFLSEDEKPLGAVIYGNGNNKEDDTEGVHYKNVFGSYFHGPILSRNANLA
                     YRLVATALRNKYGKEIVLPSYEEILSLEIPEEYGDVKSKADFE"
     gene            complement(46522..47865)
                     /locus_tag="VBE22_000395"
     CDS             complement(46522..47865)
                     /locus_tag="VBE22_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEO7201873.1"
                     /translation="MKINTALGVAAGKSAHYLLSKMGRGSTYPGSLALKFDKDILDTI
                     AKDYEIVVVTGTNGKTLTTALTVGILKEAFGQVVTNPSGANMITGIVSTFLTAKKSKS
                     GKKIAVLEIDEASLPRITQYIKPSLFVFTNIFRDQMDRYGEIYTTYQMILDGAANAPQ
                     ATILANGDSPLFNSKSVTNPVQFYGFDTDKHEPRLAHYNTEGILCPKCQAILTYRLNT
                     YANLGDYTCPNCDFERPNLDYALTRLTHLTNTSSGFVIDGQQYNINVGGLYNIYNALA
                     AVSVAEYFGVEPSQIKDGFDKSRAVFGRQETFTIGNKKCTLVLIKNPVGASQALDMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDANFETILTMNIPEIFAGGVRHSEIARRLRVT
                     GYDEKRIKQADKLQDIMAMIEQQETEHAYILATYTAMLEFREILANHNAIRKEMN"
     gene            48005..48856
                     /locus_tag="VBE22_000396"
     CDS             48005..48856
                     /locus_tag="VBE22_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEO7201874.1"
                     /translation="MDIFSAIDSKFWASIMENPWMILIHLMDIIIVAVLIYKFIKALA
                     GTKIMSLIQGVILFIIIRFVSEWIGLTTITFLMNQIVTYGVIAGVVIFAPEIRTGLEK
                     FGRTPQLFTQRSQLSSDEKLVDALVKAVAYMSPRKIGALISIERTQTLQEYIATGIPL
                     DADISSELLINIFIPNTPLHDGAVIVKDKKIATACSYLPLSESSSISKEFGTRHRAAI
                     GLSENSDALTVIVSEETGGISVALKGEFLHDLSKDSFEAILRTQLIQNQEENSKLAWY
                     NQLLRRK"
     gene            48859..49818
                     /locus_tag="VBE22_000397"
     CDS             48859..49818
                     /locus_tag="VBE22_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEO7201875.1"
                     /translation="MKKFFTNKFWLGVVSLFLAILLFLTATATSMNHQDNSKIAGASE
                     TYTHTLTDVPIDIKYDSDDYFISGYSYGADVYMSSVNRVKLDSEINEDTRKFKVVADL
                     TNMKPGTHKVPLKVVNLPSGVNATVSPTTITVTMGKKKTKEFPVYGHVNDKQIKAGYA
                     VDKMSVDVSKVKVTSDESIIDRIDHVAANIPDDKVLDDDFNKTVTLQAVTADGTVLAS
                     IIHPSKATLSVKVKKLTKTVPINLIPVGQFSDSISKINYKLSQEKAVISGTKEALEAI
                     SVINAEVDISDVTKNTEKKINLSANNVSVDPAQVTVQLTTTKK"
     gene            49872..51224
                     /locus_tag="VBE22_000398"
     CDS             49872..51224
                     /locus_tag="VBE22_000398"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEO7201876.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDRPRV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGSDGFKLDDDRELEIEALLDAKEDTLPRPSAQGLGTLVDYPEGLRKY
                     EKFMESTGIDLEGMKVALDTANGAATASARNIFLDLNADISVIGDQPDGLNINDGVGS
                     THPEQLQSLVRENGSDIGLAFDGDSDRLIAVDENGEIVDGDKIMFIIGKYLSDKGQLA
                     QNTIVTTVMSNLGFHKALDREGIHKAITAVGDRYVVEEMRKSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLTKVMKETGKKLSELASEVTIYPQKLVNIRVENNMKDKAMEVPAI
                     AEIIAKMEEEMDGNGRILVRPSGTEPLLRVMAEAPTNEAVDYYVDTIADVVRTEIGLD
                     "
     gene            51347..51718
                     /locus_tag="VBE22_000399"
     CDS             51347..51718
                     /locus_tag="VBE22_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEO7201877.1"
                     /translation="MTKEAKLLWAKHKYLVLSKSQKIYLDIRQTLKSPNCTVLDVQSL
                     IDQAVLLEESPSQVTNAYMHIWGYFKNKAERQEKEEFLTLLEKYRKTGYQRRKLLAFL
                     KQLLAKYPNSYLQNSSIFEEE"
     gene            51743..52123
                     /locus_tag="VBE22_000400"
     CDS             51743..52123
                     /locus_tag="VBE22_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201878.1"
                     /translation="MRLWHQDIIELLPRQQLLGQHRECCALRGNGWGRKHETVDYVFR
                     YSPYRLFAYHQLVMEEMMERGYRVSKEWLIAEYRGMKCPRYDTLNPVDLETPIYPEHN
                     QDYLQECLWNLKAKGIDLPINKIK"
     gene            52215..53345
                     /locus_tag="VBE22_000401"
     CDS             52215..53345
                     /locus_tag="VBE22_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEO7201879.1"
                     /translation="MLKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDAYLQALIRE
                     FRSYDITELRTLYIGGGTPTSISAVQLDYLLTELIRDLNLNTLEEFTIEANPGDLTVD
                     KIEVLQKSAVNRVSLGVQTFNDKHLKRIGRSHNEAQIYSTIDALKTAGFQNISIDLIY
                     ALPGQTMDDVRSNVAKALSLNIPHLSLYSLILEHHTVFMNKMRRGKLHLPTEDLEAEM
                     FEYIISEMERNGFEHYEISNFTKPGFESRHNLMYWDNVEYYGVGAGASGYLDGIRYRN
                     RGPIQHYLKGVSEGNARLSEEVLSKNEMMEEELFLGLRKKEGVSIGKFEQKFGTSFEK
                     RYGQIVQELQSDGLLKENNGFIQMTKKGLFLGDTVAEKFIVE"
     gene            53349..54086
                     /locus_tag="VBE22_000402"
     CDS             53349..54086
                     /locus_tag="VBE22_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEO7201880.1"
                     /translation="MGLLYRETYEVPFYESDTNHYMKLPQLLALALQISAKQSLKLGI
                     GDDIVFKRYGLVWVVTDYIIDIERLPKHAEKIVIETEAKAHNKLLCYRYFYIYGEDGQ
                     KIITISSAFVLMDFKTRKIHPVLDDITSIYQSQRIKKVIRGPKYHPIGDSKVKQYHVR
                     YFDLDMNGHVNNSKYLEWMYDVLDLDFLSSHIPKKIDLKYIKEIQYGTDIKSHWYQDG
                     LVTRHDIIGGDAIHAQARIEWQEKKED"
     gene            54087..54857
                     /locus_tag="VBE22_000403"
     CDS             54087..54857
                     /locus_tag="VBE22_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEO7201881.1"
                     /translation="MAYKGYLIDLDGTIYKGKSRIPAGERFIERLQEKGIPYMLVTNN
                     TTRTPESVQEMLRGFNVETPLETIYTATMATVDYMNDMNRGKTAYVIGEEGLKKAIAD
                     AGYVEDTKNPAYVVVGLDWNVTYDKLATATLAIQNGALFIGTNPDLNIPTERGLLPGA
                     GSLNALLEAATRIKPVFIGKPNAIIMNKALEILNIPRNQAVMVGDNYLTDIMAGINND
                     IDTLLVTTGFTTVEEVPDLPIQPSYVLASLDEWTFNEG"
     gene            54847..>55494
                     /locus_tag="VBE22_000404"
     CDS             54847..>55494
                     /locus_tag="VBE22_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO7201882.1"
                     /translation="MKDKLLIVLTWIWIISLATLATIYIAWLIYPIEIQFLKLEKVVY
                     LKAETIYYNFNKLMIYLTHPFISDLNMPSFPSSEDGLKHFADVKYLFTLAHGLFVILT
                     FPVIYFLRRGWKQKSIFLYEGFFKIAIMLPIFIVVCAFLLGFDQFFTLFHEVLFPGDS
                     TWQFNPLTDPVIWILPETFFLHCFIIFLLIYETITIILLIIGRKHLKLRKYKNKMQ"
BASE COUNT        18211 a         8713 c        11119 g        17451 t
ORIGIN      
        1 ctttttcaaa accagtttta cagataattg taaacatgta tgatttggga gatactctaa
       61 cggcgtggct tttaaatctt gcacaaccag ttacattttt gactatattt ttaggtattg
      121 gaattttata ctttatttta cctaacgcac gaattagaaa agtacgttat gtcattccag
      181 ggacattatt ttcaacgttt gttattggtt tttttagtaa tttaattagc caatacgtct
      241 taaatagggt tgagaaaatg gtagatatta aaacgtttgg ttcggtcgtt atctttatct
      301 taatgctatg gtttattttc ttagcccaca ttatgatttt aggtgcaata ctgaatgcca
      361 gcgttcaaga aattgcgaca ggaaaaattg aatcaaggcg tggagatatt atgtctctta
      421 tccaaaaatc caaagaagaa aaataagaaa agacagctct tgttagagtt gccttttttt
      481 gtctttaaaa ataacaaatt tgctcaagat gtagttgagt aaaataatta atatttgaga
      541 tacaaggctt tcaatggtgt tgattttatt taaattgtgc tgaacgaatt gtccgataat
      601 agaaggaaat tgatcaacaa aaataacagc taatatcaaa tctaatccta atgtgcctaa
      661 tctagcgatg acaaatgttt gtaagcgttg aaactgtcca attttagttt gtttaaaaac
      721 aaatcgatca tttgtaaaaa aagcaaaaag tatagctatt atgttagcaa ctattgcaga
      781 gaatggagcg ttctgcgaga aattaaaaat cccttgtcga ataactaaat aaagaaggga
      841 tgttaatagt ccgaacagta aatataagct gacttcattt tgaatacttt ttttcagtaa
      901 cgattttaat ttaaccatat ttttattata tcacgaatgc ttcaaagtaa tgaggaaatc
      961 tcccataagc ttggctttac tttttgtttg tgatatactt agtaagttgt tttacacaac
     1021 ccttgatgct tagttccctt tcatcaagca tattacaaac gttagaagtg ttaactatct
     1081 aaacgttaaa ggagaaatta tgaacacatt tacaactcgc gattacgctc acatggctat
     1141 tgtaacagct atctatatcg ttttaaccat aacgccacct tttaatgcta ttgcttatgg
     1201 tgcttatcaa ttccgtgttt cagaaatgct aaatttcttg gccttttatc accgaaaata
     1261 cctttttgcg gtaacgttag gatgtatgat ttctaatctt tatagttttg gaatgattga
     1321 tgtctttgta ggtggtggtt caaccttact ttttgtttat cttggaacca ttttattcaa
     1381 gcaatatcaa aaagattatc tatttaatgg tcttattaac aaagcattct ttttcttttc
     1441 tttcttcttt gctgcttcaa tgattacggt agcagttgaa ttgaaaattg tagcaggatt
     1501 acctttatta ttaacatggt taacgacagc tgttggtgaa ttggcttcac ttttggttgg
     1561 agcagtcctt gtggataagt tgagtagaca cgtcgatttt acaaaataaa tatattataa
     1621 agcgcgagac attgatctcg gcttttttgt ttgggtttat agttagcata gctagaaaat
     1681 gctgcaactt ttatgagaaa tgctccaatt tttactaata tccttagtct ttgtgataaa
     1741 atagaaatat ggaaaatcgt atgaatgaat tagtatcttt gttgaatcag tacgcaaaag
     1801 agtattatac acaagataat cccacagttt ctgacagtca atatgaccag ttatatcgag
     1861 aattagtaga attagaagag caacatccag aaaacatttt gccaaatagt cccacacatc
     1921 gagtgggtgg tttagtttta gaaggctttg aaaaatacca acatgaatat cctttgtatt
     1981 cattgcagga tgctttctca aaagaagaat tgatagcctt tgataaacgt gttaaagcag
     2041 agtttccaac tgcttcttat atggcagagc tgaaaattga tggtctatct gtctcgttga
     2101 cttatgtaaa tggtatttta caggttgggg caacacgtgg tgatggcaat attggagaaa
     2161 atattactga aaatttaaag cgtgttcatg atattcctct acatttagac caaagtttag
     2221 atattactgt tcgcggagag tgctaccttc ccaaagaatc ttttgaagct attaacatag
     2281 aaaaacgtgc aaatggtgag caggaatttg ctaatcctag gaatgcagca gcaggaacct
     2341 tgcgccaatt aaacacaggg attgttgcga aacgtaaatt agcaactttt ctttatcaag
     2401 aagctagccc tacgcaaaaa gaaacacaag atgatgtttt aaaagagcta gaaagctatg
     2461 gcttttcagt caatcatcat cgtttaatta gctcttctat ggagaaaatt tgggatttta
     2521 tccaaactat agaaaaagat agaatttccc tcccttatga tatcgatggt attgttatta
     2581 aggttaatag cttagcaatg caagaagagt tgggcttcac tgtaaaggca cctcgttggg
     2641 cgattgcata taaattccct gctgaagaaa aggaagcaga aatattatct gttgattgga
     2701 cagttgggcg aacaggagtt gtaacaccaa cagctaacct aactccagtt caactagcag
     2761 ggacaaccgt tagtagggcc actctgcata atgtagatta tattgccgaa aaagatatcc
     2821 gtattggaga tactgttgtt gtatacaaag caggggatat catccctgca gtcttaaatg
     2881 tcgttatgag taagcgaaac cagcaagaag tcatgcttat tcctaaacta tgtccttctt
     2941 gtggtagtga attagttcat tttgaagatg aggtggcttt acgttgtatt aatccgctgt
     3001 gccccaacca aatcaaagaa cgtttagctc attttgctag ccgggatgct atgaatatta
     3061 caggttttgg tccatcctta gtcgaaaaat tatttgatgc tcatttaata gcagatgttg
     3121 cagatattta tcgtttaagt atagaggatt tattaacttt agatggtatt aaagagaaat
     3181 ctgcaactaa gatttatcat gcaatccaat cttcaaaaga aaattctgct gaaaaacttc
     3241 tttttggcct aggtattcgt catgtgggaa gcaaggcgag ccgcctttta ttggaggaat
     3301 ttggtaattt gaggcaacta agtcaagcaa gtcaagaaag tattgcctca attgatggac
     3361 taggaggtgt tattgccaaa tcacttcata cgttctttga aaaagaagag gtagataaac
     3421 tactagagga attaacttcc tataacgtta attttaatta tctaggaaaa cgagttagta
     3481 cagatgcaca actttcaggt ttaacagtcg ttttaaccgg aaaacttgaa aagatgactc
     3541 gtaatgaagc aaaagagaaa ttacaaaacc ttggtgctaa ggtgacaggt agcgtttcta
     3601 aaaagactga tttaattgtt gcaggaagcg atgcaggaag taaactaacc aaggctcaag
     3661 atttagggat tacaatacaa gatgaagact ggttactcaa cctttaagtg aggaagaaat
     3721 gaagaaacaa aaaaaagcgc gtttaattta taacccaact tctggtcaag aaattatgaa
     3781 aaaaaatgta gcggaagttc ttgatatact tgaaggattt gggtatgaaa catcagcctt
     3841 tcaaacaacg ccaactaaaa attctgccag agatgaagct gctagagctg ctcaagctgg
     3901 ttttgattta attgttgcag caggtggaga tggtactatt aatgaagttg ttaatggtat
     3961 tgctcctttg aaacgtcgtc ctaagatggc aattattcca actggaacaa ctaatgattt
     4021 tgctcgtgcc ttaaaaatac cgcgaggtaa tcctattgaa gcaactaaat taattggtaa
     4081 aaatcaaatc gttaagatgg acattggtca agctcaagag gacaattatt ttattaatat
     4141 tgcagctgca ggtagtttga cagagttaac ttatagtgta ccaagtcaat tgaaaacaac
     4201 gtttggctat cttgcttatc tagcaaaggg tgttgaatta ttacctcgag ttcgaaaagt
     4261 accagtcaaa attactcacg ataagggtga atttatcggt gatgcttcaa tgatttttgt
     4321 tgccatcaca aattctgtag gagggtttga acaaattgca cctgatgcca aattggatga
     4381 tgggaaattt acccttattt tagtaaaaac tgcaaatctt attgagatta tgcatctcat
     4441 tcgtttggtg ctagcaggtg gaaaacatat taatgataaa cgagtagagt atattaaaac
     4501 gtcttatcta actatcgaac ccctatcaga cgagcgtatg atgattaact tagatggaga
     4561 atatggagga gatgctccca ttactctagc aaaccttaaa aatcatatta ggttctttgc
     4621 taatactgat gaaatttcgg atgatgcttt agttttagat aaagatgaat tagcgataga
     4681 agctattgca cagaagtttg ctaatgaggt agatgattta gaaggattag gagattgatg
     4741 aatggataat actgtcattg tacattacca tagccgaaag aaaaattact ttaatttaag
     4801 tttatggcag tggcgcgatg gctcagaggg tcaagatgct catttttcac ggtttgacag
     4861 tttcggtgct gttgcgatac taaaatatcc cttgccatat tttttaagcc attattatgt
     4921 gattgttaag gataaaaatt ggcatcataa aacaatagat tatcgcattg attgtactaa
     4981 aggtggtgca cgtaaagaag tttggattgt agatggggat gacaatctgt attattctcg
     5041 tcaagcagca gttagtagcc atcattatag tagacgccaa cctcatgcct acgatatggc
     5101 aatcaatagt agagcgtttg ataaaaaatg gggatttgat ggttggttag gttttgaata
     5161 ttcagaaact aaaacggctt ttagattgtg ggcccctact gcggaacgtg tagaactgat
     5221 tttatatcat tcgacagatg aaacagcaag tgtatcaaaa gttctttcta tgaaaagagg
     5281 gacagccgtc aattataaaa atcataaaga aaacacccat ggcgtctggt ttactgaact
     5341 tgaaggaaac tataattacc aagcatatac ttatcgagtc tactatagac gacgtacctt
     5401 taaaattacg agagaccctt attccattgc aacgactgca aatggtaaaa ggtctattgt
     5461 tattgctcca gaagcattaa ctccggaagg gtttaaaata agtcatggaa aagaagctaa
     5521 gtggcggctc gaaaacccca atcaagcagt catttacgaa atgcatgtgc gtgatttttc
     5581 gatatctgag acttcaggag ttaagacaga ttatcaagga aaatttaagg gattgcatca
     5641 aaaagataca gttaatcagc atggtgataa aacgaccttt gattatgtgc aagacttagg
     5701 agttaactat atccaacttc aaccaatttt cgatcatcat cagacatttg atgatgatgg
     5761 tcactatgct tataattggg gatatgatcc tgaaaactac aatgttccag aagcaagctt
     5821 ctctagtaat ccacatgaac ctgcaacgcg tattttagaa ttaaaatctg ctatccaagc
     5881 gtaccatgat gcaggcattg gtgttattat ggatgttgtc tataatcata ctttttcatc
     5941 aactgattct gcatttcaac taacggttcc agattattat tatcgaatga atcacaatgg
     6001 aacatttcaa aatgggtcag gctgtggtaa tgaaacagct agtgaaaaag aaatgtgtcg
     6061 taagtatatt cttgattcag ttttgtattg ggtgaaagaa tataatatcg atggttttcg
     6121 atttgatttg atgggactac atgatgtaga aacgatgaac attataagaa atgagctaaa
     6181 taaaatcgac cctcgtattt tggtttatgg agaaggatgg gacatgggag ctggtctcac
     6241 gcctcaaaat aaagctaaaa aagataatgc ttatcagatg cctggaattg gctttttcaa
     6301 tgatgatgta agagacgctg tcaaaggtgc ggaaatttac ggagaattta aaaaaggctt
     6361 agtttctgga aacagtacag aagatattgt tgcaaaaggc attttaggaa gtgatgagtt
     6421 agtatcatac attgatccta gtcaagtttt aaattatgtt gaagcacatg acaactacaa
     6481 cttaaatgat ttgctatggg aattacatcc taatgacaac gagaaacaac atatttaccg
     6541 agttgaagta gctagtgcta tgaatctatt gatgcaaggg atggctttca tgcaattagg
     6601 tcaggagttt cttcgaacaa agtgttatcc tacaggtgat aaaggtcaac ttactcaagc
     6661 agataaagaa agggctatga attcttacaa tgccccagac caagttaatc aggtcaactg
     6721 ggataatgtt acctttcata agagtactat taattttatt aggaaaataa ttactttgaa
     6781 gacaaactct ccttactttt cttactctag ctttgaagag attcgcaaac atgtttttgt
     6841 agagtctgct caataccatt caggtttcat aagttttaca gttgaagaac ataagaaaat
     6901 aaaaataatt ttcaatatta aaggaaaacg cttgcagaat cctttcatag gtgatataat
     6961 aatatcaaat catgaagaag gaaaagcgat agattcatta gatgatgagc tttctgtaat
     7021 gatctttgat atcacaaaat aacctgaaaa cattcaggtt atttttgtgt atattaagga
     7081 atttgatttg aatttgagtt tcttatattt tagttaattt ttcggaaata tagagggtga
     7141 aatattttca gatgcgtctg tatgaggaaa atgcattcct ctttttttgt accattgagg
     7201 aaatattttt gtcttatatt gactagtagg acctgtgagg tgagtagatg tccaataaag
     7261 atgagttata tacttttgga ataggtgaaa attttcatct ccagaattat ttaggcgtac
     7321 atagcgaaaa tgaaagcttt tgtttcagag tttgggctcc taatgctgaa aatgttcaag
     7381 ttattggaga ttttacagat tggcgaaatc gtcctctcca aatgaacaaa aatcaagcag
     7441 gagtttggga agctaatagt ttggatgcgc gcgaaggtga tttatataaa tatttggtta
     7501 ctcgtaaagg tggtcaagta gttgagaaaa tcgatcctat ggcagtttat atggaaagac
     7561 gtcctggaac tgcaagtgtt attaaagtgc ttagaaataa aaagtgggaa gatggccttt
     7621 ggatgggaag gcgcaaacgt ctggggtttc agaagcgtcc aattaatatt tacgaagttc
     7681 atgctggttc atggaaaaaa gatgattttg gacatcctat gactttttct cagttaaaag
     7741 actacttaat cccctacctt gttgagatga attatactca tgttgaattt atgcccttaa
     7801 tggcacatcc gcttgatatg agttggggat atcaattaat ggggtatttt gcttttgaac
     7861 atacctatgg tactccagaa gaatttcaag actttgtaga agcatgtcat aaaaataata
     7921 taggagttct tgtagattgg gttccaggac actttattca gaatgatgat gctttagcct
     7981 atttcgatgg aactgctact tatgagtatc aaaatcatga tcgtgcgcat aactatcgat
     8041 ggggagctct gaactttgat ttaggaaaaa atcaagtaca atcctttttg atttcgagtg
     8101 ccttattctg gattgaacat tatcatattg atggtattcg tgttgatgcg gttagtaata
     8161 tgttatatct cgattatgat gaaggaccat gggaagctaa ccaatttggg gataatcgta
     8221 atttggaagg gtattacttt ttaaggaaac ttaacaaagt aattaaagag cgtcatccta
     8281 atgttatgat gattgctgaa gagtcaacag cttctacccc aataactaaa gacttagagt
     8341 ctggaggatt gggttttgac tttaaatgga atatgggctg gatgaatgat atcctacgtt
     8401 tttatgaaga agatcctctc tataggcaat atgattttaa tttagtgact tttagtttta
     8461 tgtatatatt caatgagaac tttgtactag ctttttcaca tgatgaagta gtacatggta
     8521 aaaagagtat gatgcataaa atgtggggtg accgttataa tcaatttgct ggtttacgta
     8581 acttatatgc atatcaaatg tgtcatcctg gaaaaaaact tctctttatg ggaagtgagt
     8641 tcggtcaatt tttagaatgg aaatataatg atcaactaga atgggaaaac ttaaatgatg
     8701 atatgaacca aaagatgcaa cgctatacta aacaattgaa tcaattttat aaagatcata
     8761 agtgcctatg gcgtattgat gattcctttg atgggataga aattattgat gcagataata
     8821 aatctgaaac tgttttatct tttatacgaa aagatgataa aggggatttg ttactttgtg
     8881 tctttaatat gactcctgta gaacgcccta actttactat tggtgttcca caagcgggta
     8941 tttatgaaga agttttaaat actgaaatgg aagagtttgg aggtgtctgg aaaaaccaca
     9001 atcctgtaac gaaaacacaa gtggctacat ggaaagatta tgatcatact ttaagtttta
     9061 cattaccagc ccttggggca agtgtttgga gaataaaacg tcggcttcgt aaataagtct
     9121 gcaatttagt aacctcattt tgaaaggaaa gaataatatg aaaaatgaaa tgttagcact
     9181 cattctcgcc ggaggacaag gcactcgact cggaaaattg actcaaagca ttgcgaaacc
     9241 cgatgttcaa tttggaggtc gttaccgtat tattgacttt gcgctttcaa attgtgcgaa
     9301 ttcaggtatt aataatgttg gggttattac tcagtatcaa ccgctggaac tcaatactca
     9361 tattggaaac ggatctagct ggggactaga tggtatcgat tcgggagtaa ctgtattaca
     9421 accatattct gcaacagaag gtaatcgttg gttccaaggt accagccacg ctatctatca
     9481 aaatattgac tacattgatc gtattaatcc agaatatgtg ctcattctct ctggggacca
     9541 catctataaa atggactatg atgacatgct acagacacat aaagataatt tagcaagcct
     9601 aacagtcgct gttttagatg ttcctttaaa agaagcaagc cgttttggaa ttatgaatac
     9661 tgattcaaac gaccgtattg tcgaatttga agaaaaacct gaacatccta agtcaacaaa
     9721 agcttcaatg ggaatataca tttttgactg gaagagatta cggacagttt taattgatgg
     9781 tgaaaagaac ggcatagaca tgtcagactt tggtaaaaat gttataccag cataccttga
     9841 atcgggtgaa cgtgtttata cctataattt tgatgggtat tggaaagatg ttggtactat
     9901 tgaatccctt tgggaagcta atatggaata tattggagaa gataacaaat tacatagtcg
     9961 tgatcgttca tggaaaatct attctaaaaa tttgattgca ccacctaatt tcatgacaga
    10021 agatgcaaat gttaaagatt ctttagttgt agatggatgc tttgttgcag ggaatgttga
    10081 gcactccatt ttgtcaacta atgtccaggt aaaaccaaat gctattatta aagactcctt
    10141 tgttatgagt ggcgcgacta tcggtgaagg tgcaaagata aatcgagcta ttatcggtga
    10201 ggatgctgtt attggtgatg gtgttgtgat tgatggaagt aaagaagtag aagttattgg
    10261 ttataaagaa gtagtggggg ttccaaatga agattgataa atatactgcg attttaggaa
    10321 atactgttag ttacccagat atgcacagtt tgacggataa tcgtccgctt gcttctctac
    10381 cttttgatgg taagtatcgt ttaattgatt ttcaattatc ctctttagct aatgcaggcg
    10441 ttagaagtat ttatggtatt tttcgtggga ataatgttcg ctctgttttt gaccatatta
    10501 gaagtggtcg tgagtggggc ttaggtacat tgttgagtca ctattcttta ggattttatg
    10561 atacagacga tgatattcgt acagtcaatg gggattatta cgatcaaatt ttaacttact
    10621 tgaggcgttc gggtagcgac cagacgatat atatgagttg tgatatgctt tgtaatattg
    10681 acttaagtca agtaatccac ttgcataatg caaatggtac gcaagccacc gtggtttata
    10741 aaaaaatgcc gagatcagct attgcagagg ctaatcaagt cttgaccatt gatgaatctg
    10801 atcatgtagt gagtctaggt aaagctgtag ataatgacga taaccaaaaa atgtctgctg
    10861 agatttatgt tatcaacaca ccttggttaa tcgaaaaaat ggaagaggaa gctcaaaata
    10921 atgaaccacg taaactccgc ttcttacttc gtgatttaat tgttgaaagt aatgctttgg
    10981 cttttgaata cactggttat ttgtcaaaca tttcttctat taaatcctat tatgatgcta
    11041 acatggacat gttaacacca aataaatttt attcgctctt cttttcaaac caaaaagttt
    11101 atactaaggt gaaaaatgag gaagctactt attttgataa acaatcaaat gtctctaatt
    11161 cgcaactagc ttctggaagt attattaaag gttatttaga ccattctatc gtttctagaa
    11221 attgtctcct tgaaaatggg actcgagtag tcaatagtat tatctttcca aaagtaaaaa
    11281 tcggggaagg ggcaaccatt gaaaatacta ttattgacaa atgcgttaag gttgcacccg
    11341 gagttacatt gaaaggtagt ttagataaac cgttagtgat tcctaaattt tcagagatta
    11401 atgaggatat tatacaatga aaataatgtt tgttgcggca gagggtgctc cctttgctaa
    11461 aacaggtggt ttgggagatg tgattggcgc actcccaaaa tcacttagta aaaaaggtca
    11521 tgatgtcgtt gttgtcatgc catattatga tatggtagac caaaaatttg gagaccaaat
    11581 agaaaacctc atgtatttct atacagatgt tggctggcgt catcaatatg taggagttaa
    11641 gagactttct caagataatg ttacgttcta ctttattgat aatcaatatt atttttatag
    11701 aggtcatgtc tatggtgatt gggatgatgg ggagcgtttt gcttatttcc aattagctgc
    11761 tttagagttg atggagaaaa tcaattttat tcccgatgtc ttacacgtcc atgattatca
    11821 tacagcaatg attcctttct tattgaaaga gaagtaccac tggattcagg cctataataa
    11881 tataagaaca gtttttacta ttcacaatat tgaatttcaa gggcagtttg gtccagaaat
    11941 gttaggagat ttatttggag ttggcgctga gagatatgaa gatggaactc ttagatggaa
    12001 taattgtctt aattggatga aagcagcaat tctttactca gatagagtga caacggtatc
    12061 accaagctac gcaaatgaaa taaaaactcc agaatttggt aaaggtttag accaaattat
    12121 gcgtatggaa gcaggcaaat tatctggaat tgtaaatggt attgacagcg atttgttaaa
    12181 tccagaaaca gatgcttttt tgccatatca tttttcaaaa agcaacttag agggcaaaat
    12241 taaaaataag ttggctttac aggaaaattt gggcttacct caagataaaa atgtaccatt
    12301 aattggtatt gtttcacgac ttaccgatca aaaaggattt gatattattg cttctgaatt
    12361 ggacaatatg cttcaacaag atattcaaat ggttatttta ggaacaggct atcaccattt
    12421 tgaagaaacg ttctcttatt ttgctagtcg ttaccctgaa aaactatctg ctaatattac
    12481 ttttgactta agattagcgc aacagattta tgcggcttca gatatcttca tgatgccaag
    12541 tgcttttgaa ccttgtggtc tctcgcagat gatggctatg agatatggta gcttgccctt
    12601 ggtacatgaa gttggaggat taaaagatac tgtggtagcc ttcaatcaat ttgatggtag
    12661 tggtacaggt ttttcattta atcatttttc aggctattgg ttaatgcaaa ctctaaaact
    12721 agcattagaa gtttataatg attatcctga agcgtggaaa aagttacaat ggcaagctat
    12781 gtcaaaagat ttttcatggg atacagcatg tgttgcttac gaacaactgt atcagcaact
    12841 acaataatca ccttatttta gtgattagtt gataagctta tcgaatctct atgctaaacg
    12901 tttcttatgt aaaaccactt tagtatagag attttattat cctataaggt aaaatattat
    12961 taatgtaaag agacactata tcaaaaataa agtgttgtca tatcttaata agggattaaa
    13021 ttaaagctaa aaaaactaaa aattgttatt tgaaaaggat tgtgtaaacc atctcaaact
    13081 atttcatgaa aacgtaatca aatgaggaaa ttcattgaca tgcacaagaa ataggaataa
    13141 aataacggag taaatatttt ttaaaggaga attctattat gaatttagct attttagcac
    13201 ttggtttcgc cgtgatgggt gtttcaatcg gtgaaggaat tttggttgca aatattgcca
    13261 aatcagcggc tcgtcagcct gaaatgttta gcaaactgca aacattgatg tttacaggtg
    13321 tggccttcat tgaaggtacc ttcttcgttc tatttgcgtt tacattctta gtaagataga
    13381 cgacaattta gaaaaaaagg agggatgagg attggaatct acaagtaatc caaccgtatc
    13441 ttttttaggc attgattttg atttaaccat cctagctatg tctctcttga caattactat
    13501 tatctttatc ttagtcttct gggctagtcg taagatgaca ataaaaccaa aaggtaagca
    13561 gaatgtctta gaatatgtct atgagcttgt taataataca atctcacaaa atttaggaca
    13621 ttatactaaa aattatagtt tattgatgtt tattttattc agttttgtct tcgttgctaa
    13681 caacttagga ttgatgacat cattgaaaac acatgagcat aatttttgga catctccgac
    13741 cgctaatttt ggtgtagata ttacattatc attattagta gcatttattt gtcatattga
    13801 aggtattcgc aaaaaaggaa ttggtggtta tctcaaaggt ttcttatcac caactccagc
    13861 aatgcttccg atgaatttat tggaagaagt tactaatgtt gcttcattag cattgcgtct
    13921 ttttggtaat atcttttctg gtgaagttgt cactggatta cttttacaac ttgcagtttt
    13981 atcaccattt acaggtccac tagcttttgc attgaatatc gtttggacag cattctcaat
    14041 gtttattggt ttcatccaag cgtatgtgtt cattatcttg agctcaagtt atattggaca
    14101 caaagttcat ggtgacgaag aagaataaga aaagagagga gaaatatgtc aatacttatt
    14161 aatagtacaa cgattggtga tatcattatc gtatctggct ctgtgcttct cttatttatc
    14221 ttaattaaga cttttgcatg gaaacaaatc acaggtattt ttgaagcccg tgaacaaaag
    14281 atagctaatg atattgatac tgctgaacag gcacgtcaac aagctgaagc ttttgcaact
    14341 aagcgtgagg aagaactatc taacgctaaa actgaagcta atcaaatcat tgataacgct
    14401 aaagagacag gtcttgcaaa aggtgatcaa attatttcag aagcaaaaac tgaagctgat
    14461 cgattaaaag aaaaagcaca tcaagatatt gcccaaaata aggcagaagc actagcagat
    14521 gttaagggtg aagtagcaga tttaacagtt ttattagctg aaaaaattat ggtatcaaat
    14581 cttgataaag aagctcagag taatctcatt gatagctata ttaaaaagct aggagatgcc
    14641 taatgaataa aaagacgcag gctctaattg agcaatattc aaaaagcctt gttgaagttg
    14701 ccattgaaca taaaattgtt gaaaaaatcc aacaagaagt agcagcttta attgatattt
    14761 ttgaaacaag cgagcttgag ggtgtcctgt caagtcttgc cgtttcacat gatgaaaaac
    14821 aacattttgt gaagacattg caaacatcat gttcaactta tcttgttaat ttccttgagg
    14881 ttattgtaca aaatgaacgt gaagcacttc tatatcctat tttgaaatca gttgatcagg
    14941 aacttatcaa agtgaatggt caatatccaa ttcaaattac aactgcagtt gctttgtctc
    15001 cagaacaaaa agaacgtctt tttgatattg ctaaaactaa attagcttta ccaaatggac
    15061 agttagttga gcacattgac ccatctattg ttggtggatt tgttgtcaat gcaaacaata
    15121 aagtaattga tgcaagtgtt cgtaatcaat tacatcaatt taaaatgaaa ttaaaataga
    15181 aagtggtgtg acttttggca attaatgcac aagaaattag cgctttaatt aaaaagcaaa
    15241 ttgaagactt ccagccaaat tttgacgtca cagaaactgg tattgttaca tatatcggtg
    15301 atggtattgc tcgtgctcgt gggctagata atgctatgag tggagaactt cttgaatttt
    15361 caaatggtgc gtacggtatg gctcaaaacc ttgagtctaa tgacgtgggt atcattattt
    15421 tgggtgattt ctcagaaatt cgtgaaggtg atgtggtaaa acgtactggt aaaattatgg
    15481 aagtaccagt aggggaagct atgattggac gtgtcgtcaa tccgcttggt caaccagttg
    15541 atggactagg tgaaattgag acgacagcaa cacgcccagt tgaaacacca gcacctggtg
    15601 ttatgcaacg taaatcagtt tttgagcctc ttcaaacagg tctcaaagcg attgatgctt
    15661 tggttcctat cggacgtggt caacgcgaat tgattatcgg tgaccgccaa actggtaaga
    15721 cgtctgtagc gattgatgca atcttgaatc aaaaaggtca agatatgatc tgtatctatg
    15781 tggctatcgg tcaaaaagag tctacagtac gtacacaagt tgaaactttg cgtaaatacg
    15841 gtgcccttga ttatacaatc gttgtgacag cttcagcatc acaaccatct ccattacttt
    15901 tcattgctcc atatgcagga gtagcaatgg ctgaggaatt catgtataac gggaaacacg
    15961 ttttgattgt ttatgatgat ttatcaaaac aagccgtagc ttatcgtgaa ctttcgcttc
    16021 ttcttcgccg tcctccaggt cgcgaagctt acccagggga tgttttctac cttcatagtc
    16081 gtcttttgga acgttcagct aaagtatcag atgctttagg tggaggttcg attacagcgc
    16141 ttccgttcat tgaaacacaa gcaggtgata tctcagcata tattgctaca aacgttattt
    16201 caattactga cggccaaatt ttcttgcaag aaaatctctt taactcaggt attcgtccag
    16261 cgattgatgc aggttcatca gtatctcgtg ttggtggtgc agcgcaaatt aaagcgatga
    16321 agagagttgc aggtacgctt cgtctagatc ttgcttctta tcgcgaactt gaggcattca
    16381 ctcaatttgg ttcagacctt gatgcagcaa cacaagcaaa actaaatcgt ggtcgtcgca
    16441 cagtggaagt gttaaaacaa ccattacaca aaccattacc agttgagaaa caagttgtga
    16501 ttttatatgc tctaacacat ggattccttg atgatgtacc tgtaaatgat attttagctt
    16561 ttgaagaagc tttgtatgat tactttgatg ctcattatga taatctcttc gaaacaatcc
    16621 gcacaacaaa agatttacca gaagaagcag aattagatgc tgctattcaa gcatttaaag
    16681 atcaatcaca atttaaataa taggagggtt ctaatatggc aggctctctt agtgaaatta
    16741 aagacaagat tctctctaca gaaaaaacaa gtaagattac tagtgcgatg cagatggtct
    16801 catctgccaa gttggttaaa tctgaacaag ctgctagaga ttttcaagtg tatgcctcta
    16861 aaattcgtca aattactact aacctcttga agtcagactt agtatctgga tcagataacc
    16921 ctatgttagc ttcacgtccg gtgaagaaaa caggttatat tgttattact tctgataagg
    16981 gtttagttgg tggttacaat tcaaaaatct taaaagcgat gatggacact ataactgact
    17041 atcatactga gaatgatgat tatgccatta tttcaattgg tagcgttggt tctgattttt
    17101 tcaaagcacg tggaatgaat gtttctttcg aattacgtgg tttagaggac caaccaagtt
    17161 ttgatcaagt tggtaaaatt attgctcaag cagttgaaat gtacaaaaat gagttgttcg
    17221 atgaattata tgtttgctac aatcaccatg ttaacagttt aactagccaa gtcagaatgc
    17281 aacaaatgct tcctatcaaa gaattggatg cagacgaggc ttctgaggat agagtgatta
    17341 ctggttttga acttgagcca aaccgtgaag ttatattgga gcaattattg cctcaatata
    17401 ctgagagttt gatttatgga gctattattg atgctaaaac agcagagcat gcggctggta
    17461 tgacagctat gcagactgca actgataatg ctaaaaatgt tatcaatgat ttaactattc
    17521 aatataaccg tgctcgtcaa gcagctatca cacaggaaat tacagaaatt gttgcaggtg
    17581 ctaatgcact tgaataatta attagtctat agaaaaaata gtgaacaaag gtattttaaa
    17641 tcctttgtat catatgataa ggagaaatac atgagctcag gcaaaattgc tcaggtagtt
    17701 ggacctgtcg ttgacgttgt atttgcaagt ggcgacaaac ttcctgagat taataatgca
    17761 ttgattgtct ataaaaatgg cgataagtca caaaaagtag tacttgaagt tgctcttgaa
    17821 cttggtgacg gcctcgttcg tacaatcgct atggaatcaa ctgatgggct tacacgtggt
    17881 ttggaagtat tagatactgg tcgcgcaatt agtgtgccgg ttggtaagga tactttgggt
    17941 cgtgtcttca acgttcttgg agatgctatt gaccttgaag agccttttgc agaagatgca
    18001 gaacgtcaac caatccataa aaaagcacca tcgtttgatg aattatcaac atcatcagaa
    18061 atcttagaaa caggtattaa agttattgac ttattagcac cttacttaaa aggtggtaaa
    18121 gttggacttt tcggtggtgc aggtgttggt aaaaccgttc ttattcaaga attaatccac
    18181 aacatcgccc aagaacatgg tggtatttca gtatttactg gtgtaggaga acgtactcgt
    18241 gaagggaatg acctttattg ggaaatgaaa gaatctggcg ttattgaaaa aacggctatg
    18301 gtctttggtc aaatgaatga accaccagga gcacgtatgc gtgtggcact tactggtctt
    18361 acaatagctg agtacttccg tgatgtagaa ggacaagatg tgcttctctt cattgataat
    18421 atcttccgtt tcacacaagc tgggtcagaa gtgtcagcgc ttttaggtcg tatgccttca
    18481 gccgttggtt atcaaccaac acttgctaca gaaatgggac aattacaaga gcgtatcact
    18541 tcaactaaaa aaggttctgt tacctcaatc caagctattt atgtgccagc agatgactat
    18601 actgaccctg cgccagctac agcctttgct cacttagatt caacaactaa ccttgaacgt
    18661 aaattgacac aaatggggat ttatccagcc gttgacccat tagcttcaag ttctcgtgca
    18721 ctcacaccag aaattgttgg agatgagcac tatgaagttg caacagaagt acagcgtgtt
    18781 cttcaacgtt accgtgagtt gcaagatatc attgcgattt taggtatgga tgaattatca
    18841 gatgaggaaa aaacgttagt tggacgtgca cgtcgtatcc aattcttcct ttctcaaaac
    18901 ttcaatgttg cagaaacatt tactggtcaa ccaggttcat atgttccggt tgaagaaact
    18961 gttcgcggat tcaaagaaat cttagatggt aaacatgatc aaattccaga agatgccttc
    19021 cgtatggttg gtggtattga ggatgttatc gcaaaagcag aaaaaatgaa ctattaatga
    19081 ggtgatgcta tggcacaatt gactgttcaa gtagtaacac cagatggtat tagatatgac
    19141 catcacgcta gtctcattac tgttagaacg ccagatggtg aaatgggaat tttaccaggt
    19201 cacataaatt tgattgctcc ccttattgtt catcagatga agattaatcg ttctcatcaa
    19261 gaaggtgtag actgggtagc tgttaatggt ggtataattg aagttaatga ggatcaggtg
    19321 acaattgttg cggattcagc agaacgagca cgtgatatag accttaaccg tgcagaacgg
    19381 gctaaggaac gcgcagagcg tgcattagaa aaagctcaaa caactcagaa tattgatgag
    19441 atgcgtagag ctgaggttgc tcttcgtcgc gcaattaacc gtattagcgt cggtaaaaag
    19501 tagaaaaatg gtctaattag ttttgtgact aattaggctt ttttagtgta taatagaagg
    19561 catgaatatg atattaaata tagtaactat agttagtaat tttattttta ttgccctttt
    19621 ttatcaactt tttgtagatt tatttgactg gtctaaaatg attaaaatgt cccctcaaaa
    19681 catctcaaaa ttaaaagttt ttattttgtt gataagtata gttggtggct atgtggttag
    19741 tcatttttta ttggaagtga ttcagttgtg tcaatcaatc ttttgggctc tccaataaag
    19801 gaaatattag ctgaactatg atataatcaa cttactattg ttataagaat ggagattcaa
    19861 atggataaaa ttattgtgga aggtgggcaa acacggcttc aaggtcaagt tgttattgaa
    19921 ggagctaaaa atgcagtttt acctctttta gcagccacaa ttttaccaag ccaagggaaa
    19981 acattattga ccaatgttcc tatcttgtct gatgtattta caatgaacaa tgttgttcgt
    20041 ggtttagata ttcaagttga ttttaattgc gataaaaaag aaatcttagt ggatgcctca
    20101 ggtgatattt tagatgttgc accttataaa tttgttagtc aaatgagagc ttctattgtt
    20161 gttttgggac ctattttagc tagaaatggc catgctaagg tttcaatgcc aggcggttgt
    20221 acaattggta gccgaccaat tgaccttcat cttaaaggtt tagaagctat gggagctacg
    20281 attacacaaa atggtggtga tattactgcc caagctgaaa agcttaaggg ggctaatata
    20341 tacatggatt ttccctcagt cggtgccact caaaacttga tgatggctgc tacacttgct
    20401 tcaggcacaa caacgataga gaatgctgca cgtgaaccag aaattgtaga ccttgcgcag
    20461 ttactaaata aaatgggagc taaagtcaag ggtgctggta cggaaacatt aacgatcatt
    20521 ggtgttgatg ctttacatgg tacagaacat gatgttgtcc aagatcgtat tgaagctgga
    20581 acctttatgg tggcagctgc tatgacttct ggaaatgttc tagtaaagga tgctatttgg
    20641 gaacataacc gtcctttaat ctctaaattg atggagatgg gtgttgaagt gtcagaggaa
    20701 gaagatggta ttcgtgtaaa agctgatact aaaaaattga agccagtgac tgtgaaaaca
    20761 ctccctcatc caggatttcc tactgatatg caagctcaat ttacagctct aatggcagta
    20821 gttaatggcg aatcaactat gattgagaca gtttttgaaa atcgttttca acatttggaa
    20881 gaaatgcgtc gcatgggatt acaaacggaa attttacgag atactgcaat gattcatggt
    20941 gggagagctt tacaaggtgc accggtcatg tctactgact tacgagctag tgcagcttta
    21001 atcttggcag gaatggttgc gcaagggcaa acagtagttg gccaattaac ccatttagac
    21061 cgtggttatt atcaattcca tgaaaaatta gcggctcttg gtgctaatat taaacgtgta
    21121 agtgaggcgt aatatggcat ctagtggttg gaaatatgta ttaaaacaaa tcggtctaat
    21181 agtattagtc atactattag ccttgctgtt tttagcggtt ggattgatgt tgggttatag
    21241 tgtttttggt gacggagagc atgcttactc tattttatcg ctggataaat ggcaaaatat
    21301 tattggaaaa tttttaggaa aatagaaaga gccactataa gttatttgat gcttatagtg
    21361 gtttcctttg agagttaatt ttagcagtag aataatacaa tgacaaaaaa acaaaaacaa
    21421 ataacaactt cgattgtaac tttgcttttt tcattactag caatagggtt aggtgaaagc
    21481 aaatctttgc ctaatgatca cattttaaaa caggtgacag atttaattat tagtaatgaa
    21541 tctggcaaaa atacttccct taaaaatgta tcaggaaccc ctagtagaga gttatctgaa
    21601 tctgttttga ctagcaatgt taaaaaacaa ttgggaacaa atatagcttg gaaccagtca
    21661 ggtgctttta tcattaatca aaataaaaca gatttgaatg ctaaagtgtc aagtgcacca
    21721 tatgctatta atgaaataaa gaaggttaat aaccaaattg ttccgactaa agcaaatgcc
    21781 ttattaacaa aggcaactcg tcaatatcga aatcgagaag aaacagggaa tggtagaact
    21841 tattggaaac cagctggatg gcatcagata aatggattaa aagggagcta taaccatgct
    21901 gtggacagag gccatttaat tggttatgct ttagtaggga gtttaagagg atttgatgcc
    21961 tccacaagta atcctaaaaa tattgccaca caagcagctt gggcaaacca agctaatagc
    22021 aaccaatcaa cagggcaaaa ttattatgaa actttagttc gtaaagcatt ggatcgtcat
    22081 aaaactatta ggtatcgtgt gactttaatt tatgatagag ataatctttt atcttcggga
    22141 tcacatattg aggctaaatc ttcagatgga agtttagaat ttaacgtttt tattcctaat
    22201 gtacaatcgg gattattatt tgattatgca acaggaaaag tgaagcagac aaagtaatct
    22261 ttatctaata gtgcttgtta aagactgaaa aatttgataa aatagtcttt aattgaatag
    22321 aggtctgtga gactagtaaa ccaagggaag ctgcttaggg agttcagccg tgactgcaag
    22381 ctgaatcagt gaaagagggt ggaattcact cacaaatagg actataatta aggtctgatt
    22441 ggtcgatcag atagaaaacg gatggtaccg cgtgtcaacg ctccgattaa ggagtagtgg
    22501 cacgcttttt ttgttttcaa agctacttat tttgacttgg aggaaagatg gatttacaaa
    22561 aacaattaga agagttgaaa atatcaactc aagaaaagct taaagaaatg actgggaacc
    22621 acactaaaga attacaagac ttacgtgtac aagttttagg aaaaaaaggt tctctaacag
    22681 aattactaaa aggtctgaaa gatttatcta atgatttacg accagttgtt ggaaaacaag
    22741 tcaatgaagt acgtgatatt ttaacaaaag cgtttgagga acaagctaaa gtagtggaag
    22801 ctgcgaagat tcaagcacag ttagagtctg agagtgttga cgttaccttg cctggtcgtc
    22861 aaatgacatt aggtcaccgt catgtcttaa cacaaactag tgaagagatt gaagatatct
    22921 ttttaggtat gggattccaa gtggttggtg gttttgaagt tgagaaagac tactataatt
    22981 ttgaacgaat gaatttaccc aaagatcatc cagcccgtga tatgcaagat actttttata
    23041 ttacagaaga aatcttgcta cgtactcata cttcgcctgt tcaagcacga acaatggacc
    23101 aacatgattt ttctaaaggg cctttaaaaa tgatttctcc tggacgtgtt ttccgtcgtg
    23161 atactgatga tgctactcat agccatcaat tccatcaaat tgaaggctta gttgttggag
    23221 aaaatatttc aatgggcgat ctcaaaggaa cacttcaact gatttctcaa aaaatgtttg
    23281 gagcagagcg taaaatccga cttcgtcctt catatttccc attcacagaa ccttcagttg
    23341 aagttgatgt ctcttgcttc aagtgcggag gtaaaggatg taatgtctgc aaacagacag
    23401 gttggattga gatattgggt gctggtatgg ttcacccaag tgttttggaa atgtctggta
    23461 ttgattctga gaaatactcc ggttttgcct ttggtttagg tcaagaacgt attgcaatgt
    23521 tacgctacgg tatcaatgac attcgtggct tctatcaagg tgatgttcgc ttcacagatc
    23581 aattttaaga ttttagatag caagtagtat cattggtgtg atgataaatt tatgttaacg
    23641 ctattacctt actcaatagg aggttttcta atgtctcaaa ttacttttaa aaaggttggc
    23701 cttgataatg tgaatatttt acagaatctt gctatagaaa cctttcggca gacgttttct
    23761 catgataata gtgaggaaca acttcaagca tttttcaatg agtcttacac tttaccagta
    23821 ttaaaatcgg aaattaccca tgctgaatct gatacttatt ttgtttatct tgatacagat
    23881 ttagtgggtt atttaaaagt aaactgggga agtcagcaaa ctgaaaaaga tcttgataaa
    23941 gcatttgaaa ttcaaaggat ttatcttcta gatgcctacc aagggaaagg tataggtaag
    24001 gctactttcg aattcgcttt ggatttagct tataaatcag gcttagattg ggcttggtta
    24061 ggtgtttggg agtttaatca taaagctcag gcattttacg ctaaatacgg atttgaaaaa
    24121 ttttctgagc accaattttc agtaggtgat aaagtggata cggattggtt attaagaaaa
    24181 agtttacact aaaaactaca aagcacttgt ataaaaataa aagaaaataa atagagagaa
    24241 attacatgtt agtatcgtat aaatggttaa aagagttagt ggatgttgat gttacaacag
    24301 cagaactagc tgagaaaatg tcaaccactg gtattgaggt tgaaggtgtg gagacaccag
    24361 cggaaggttt atcaaaatta gttgtaggtc atattgtttc gtgtgaagat gttccagata
    24421 cccacttaca tttgtgtcaa gttgatacag gggatgatga acttcgtcaa gttgtctgtg
    24481 gtgctccaaa tgtcaagact ggtattaatg ttatcgtagc ggttcctggt gctcgtattg
    24541 cagataacta taagattaaa aaaggtaaaa tccgtggtat ggagtcactt ggaatgattt
    24601 gttcacttca agagttgggc ttatcggaaa gtatcattcc aaaagagttt tctgatggta
    24661 tccaaatttt acctgaggga gctatacctg gtgatagtat tttctcttat cttgacttag
    24721 atgatgaaat tattgaatta tcaattacac caaaccgtgc agatgctctt tcaatgcgtg
    24781 gtgttgccca tgaggtagca gcaatttatg ggaaaaaagt tcattttgaa gaaaaaaatc
    24841 tcattgaaga agcagaaaga gcagctgata agatttctgt tgtcattgaa tcagataaag
    24901 ttttatctta ctcggctcgt attgttaaga atgtaacagt tgcaccaagt ccacaatggc
    24961 ttcaaaataa actgatgaat gctggtatac gtccaattaa taatgttgtt gatgtgacaa
    25021 actatgttct tttgacttat ggtcaaccaa tgcatgcttt tgattttgat aaatttgatg
    25081 ggacaactat agtagcacgt aatgctgaga atggggaaaa gttaattact cttgatggtg
    25141 aagagcgtga cttaatagct gatgacttgg tcattgcggt aaatgatcaa ccagttgctt
    25201 tggcaggcgt tatgggagga caatcaactg aaattggtag ttcttctaag acagtggtcc
    25261 ttgaagcggc agtatttaat ggaacaagta tccgcaaaac aagtggaaga ctcaaccttc
    25321 gttcagaatc atcttctcgc tttgaaaaag gtatcaatta tgatacagtt tcagaggcta
    25381 tggattttgc ggctgcaatg ttacaagaat tggcaggtgg tcaagttctt tctggtcaag
    25441 ttacagaagg agttcttcca actgagccgg tagaggtatc aacgactctt ggttacgtta
    25501 atactcgttt gggaacagaa cttacctata ctgatattga agaagtattt gaaaaactcg
    25561 gctttgctat ttctggttca gaagtgaaat ttactgtgtt ggtaccgcgt cgtagatggg
    25621 atattgccat tcaagctgac ttagttgaag agattgcccg tatttatggt tacgaaaaat
    25681 taccaacaac attgccagaa gccggagcga ctgcaggaga attaacgagt atgcaacgct
    25741 tacgtcgtag agtccgtacg gttgctgaag gagcaggatt atctgaaatt atcacctatg
    25801 ctttaacaac tccagaaaaa gcagtgcagt tctcaactca agcaactaat ataactgaac
    25861 ttatgtggcc gatgactgtt gatcgctcag cacttcgcca aaatgttgtt tcaggaatgc
    25921 ttgatacaat tgcttataac gttgctcgca aaaacagtaa ccttgcagtt tatgagatag
    25981 gaaaagtttt tgaacaaact ggtaatccaa aagaagattt accaactgaa gtagagacgt
    26041 ttacttttgc cttaacaggt ctagtagagg aaaaagattt ccaaacaaaa gccaagccag
    26101 ttgatttctt ctacgccaaa ggtattgtag aggcgctctt tattaaatta aaattagatg
    26161 ttacttttgt agcacaaaaa ggtttagcaa gtatgcaccc aggtcgtaca gcaactatct
    26221 tattagatgg gaaagagatt ggttttgttg gtcaagtaca cccacaaact gcaaaacaat
    26281 atgatattcc tgaaacttat gttgctgaaa tcaatctatc aactattgaa agtcaaatga
    26341 accaagcgtt gatttttgaa gatattacga agtatccgtc tgtatctcgc gatattgcat
    26401 tattacttgc agaatctgtt agtcatcatg atattgtgtc agcaattgag acatcagggg
    26461 taaaacgtct aacagcgatt aaactgtttg atgtctacgc tggaaataat attgcagaag
    26521 ggtataaatc aatggcttat agcttaactt tccaaaaccc taatgacaat ctaacggacg
    26581 aagaagtggc taaatatatg gaaaaaatca ctaaatcact cgttgagaaa gttaatgcag
    26641 aaataagata atttatattt tacgttgaaa aataaataaa aaacagcatc aattaagatg
    26701 ctgttttttt atttaaaatt tatagatgtt ctacggagaa agtatcaccg tgttggatgt
    26761 caccatattg aaagcctttg ttaaaccaac gttggcgttg ttcagctgtt ccatgggtaa
    26821 agttatcagg cactaatttt ccgtaggttt ctttctgaag ggtatcgtct ccgacggcgt
    26881 gggcagcatt catggcctct tcaaagtctc cttgttctaa gagatttttt cccctgatgt
    26941 agtgagccca tacccctgca taataatctg cttgaagttc tagccgaaca tttaaagcat
    27001 ttgcttcttt cttagtaagt ccgtgtcgca ttgtattata cttatccata atgcctaact
    27061 ctgtttgaat gtggtgacca acttcgtggg cgatgacgta ggccatagca aaatcaccag
    27121 tagcaccata tttatgtgat aattcattgt aaaaagaaat atcaagatag atttttttat
    27181 ctgctgaaca ataaaatggt cctgaagcag attcacctat accacaacct gtttgaattg
    27241 aattggtgta aagaacaagt tttggttcct tataatttcc aaaaccttgg gtttggaatt
    27301 cttgtgacca gaaatcctca gttgagccaa ggactttatt aacgaaatcg atttgttctc
    27361 tcgttgcgct attatcaaca gaacgtgaga cattctgaga ttggtaacta gaaggtgagg
    27421 atgagtcatt aaaaatgctg gttagtcccc ctccgccaag tagcagtaag atgattaaaa
    27481 ccacaagctt ggttttccaa ctccctcgca tcaataaaag ttgaagaatc ggtaatccac
    27541 ttcctccgct agagaatgaa cctccgctac tggagcgacg atcttcaaca ttgtcgcttt
    27601 ttcttaggtc atctattttc ataagtatct ccaaagtgtt ttataggctc attataaact
    27661 attttaataa gaaatgcttg aatttttaac taaatatctt cccctagatt tgaagctatt
    27721 tttggtataa tattaataaa ctgtaaagga taagttgaag aaggtttact tctcagttga
    27781 cttattagat aggagattca tgaaattact ttatactgat attaatcatg atatgacaga
    27841 aatcttagtc aatcaggccg cgcatgctgc tgaagctgga tggcgtattt tttatattgc
    27901 tccaaactct ttatcttttg aaaaagagcg tgcagtatta gagaaccttc ctcaagaagc
    27961 ctcttttgct attacaatta ctcgctttgc tcaattggca cgttatttta ctttaaacca
    28021 gcctaatcaa aaagaaagtt taaacgatat tggattagca atgatctttt atcgtgcttt
    28081 agcaagtttt gaagatggac aattaaaagt ttttggacga ttgaagcaag atgcttcctt
    28141 tatcagtcaa ttggtagacc tatacaaaga attgcagaca gcaaatctga gtattttaga
    28201 tttaaaatat ttacattctc ctgaaaaatt tgaagattta ttagctattt ttctagtggt
    28261 ttcagatctt ttaagagaag gagaatacga caatcaatct aaaattgcct tttttacaga
    28321 acaagtaagg tcaggccagc tagatgtgga tttaaagaac actatcctta tagtcgatgg
    28381 ttttacccgt ttctcagctg aagaagaagc tttaattaaa agtctgagtt cacgttgtca
    28441 agagattatc atcggagctt atgctagcca aaaagcgtat aaggctaact ttactaatgg
    28501 aaatatttat tctgcagggg ttgatttttt aagatatcta gcaacgacat ttcaaacaaa
    28561 accagaattt atcttgtcta agtgggaaag taagtctggt tttgaaatga tttcgaaaaa
    28621 tatcgaaggt aaacatgatt ttacaaatag tagtcacatt ttagacgaca ctgctaaaga
    28681 ttgtataaca atttgggagt gcattaatca aaaagatgaa gtggaacatg tggctagagc
    28741 tatacgtcag aaactttatc agggatatcg ctacaaagat attttagttt tactaggaga
    28801 tgtagatagt tataaactac aattaagcaa gatttttgag cagtatgata ttccttatta
    28861 tttcggtaaa gctgaaacaa tggctgcgca ccctttagtt cattttatgg attccttgtc
    28921 tcgaataaaa cgttatcgat ttagagctga agatgtttta aatcttttta agacaggtat
    28981 ttatggagag attagtcaag atgatttaga ttattttgag gcttatatct catatgcaga
    29041 cattaaaggt cctaagaagt tctttacaga ttttgttgtg ggggctaaaa aatttgattt
    29101 agggcgtctt aacactattc gtcagtccct ccttgctcca ctggaaagtt ttgttaaaac
    29161 gaaaaaacaa gacggtataa aaacactcaa ccaattcatg tttttcctta ctcaggtagg
    29221 tttgagtgac aatttatcgc gattagttgg tcaaatgtct gaaaatgaac aagaaaaaca
    29281 tcaagaagtt tggaaaacat ttacagatat tttagagcag tttcaaacaa tatttggtca
    29341 agaaaaatta aatttagatg aatttttatc gttattaaat tcaggtatga tgcaagccga
    29401 gtatcgtatg gtccctgcta ctgttgatgt ggttactgtt aagtcttacg acttggtaga
    29461 accacatagt aatcaatttg tctatgccct aggcatgaca caatcacatt ttccaaagat
    29521 tgctcaaaac aaatctttaa tatctgatat tgaacgtcag cttataaatg atgccaatga
    29581 cactgatggt cattttgaca ttatgacgcg agaaaatttg aagaaaaacc attttgctgc
    29641 tctctctctt tttaatgctg ctaaacaagc attagtgttg actataccac aattattgaa
    29701 tgagtctgaa gatcagatgt caccatatct tatcgaacta agagatatag gtgttccttt
    29761 taatcataaa ggacgccaat cgttaaaaga agaagctgat aatattggga attacaaagc
    29821 tttattatcg cgtgttgttg acttgtatcg ctctgctatt gacaaagaga tgactaaaga
    29881 aaaacaaact ttttggtcag ttgctgttcg ttatttgcgt cgtcaattaa cttctaaagg
    29941 gattgagata ccaataatta cagatagtct tgatactgtg acagtttcat cagatgtgat
    30001 gaccagacgc ttcccagaag atgacccact taaattatcc tcatctgctt tgacaacttt
    30061 ttataataat caatataagt attttttaca atatgtgtta ggtttggaag aacaagactc
    30121 aattcatcca gatatgcggc accatggaac gtaccttcat cgtgtttttg aaattctgat
    30181 gaaaaaccaa gggatagaat cttttgaaga gaaactcaac tctgcaatta ataaaaccaa
    30241 tcaagaagat gttttcaaat ccttgtattc agaagatgca gaaagtcgtt attctttaga
    30301 aattttagaa gatatcgcgc gtgcaactgc aaccatatta cgacaagata gtcagatgac
    30361 tgttgaaagt gaagaagaac gatttgaact aatgattgat aatactataa aaattaacgg
    30421 tattattgac cgtatcgatc gtttatctga tggttctttg ggtgtcgtgg actataaatc
    30481 aagcgctcaa aaattcgata tccaaaaatt ttataacggt ctaagtccac aattagttac
    30541 ctacattgat gctatcagtc gtgacaagga ggttgaacaa aaaccaccga tttttggagc
    30601 tatgtattta catatgcaag aacctaaaca agacttgtct aaaattaaaa atttagatga
    30661 tttagtaaca aaaaatcatc aagctttaac ttataaagga ttattttcag aagctgaaaa
    30721 agaattttta gcaaatggga aataccatct caaagattcc ctttattcag aggctgaaat
    30781 tgctatttta caagcacata accaattgct ttacaaaaaa gcatctgaga ctattaagtc
    30841 aggtaagttt ttgatcaatc catatacaga agatgctaaa acagttgacg gtgatcagtt
    30901 taagagtatt acggggttcg aggcggatag gcatatggcg cgtgccagag cgctttataa
    30961 attacctgct aaggaaaaac gtcaaggttt cttaacatta atgcagcaag aggaggaaaa
    31021 tgatgacctt taagcctttc ttaaatccag aagatattgc tgttattcag acagaagaaa
    31081 aaaattctga taaaaaacaa aagcgaacgc ctgaacaaat tgaagccatt tatacttttg
    31141 gtaacaatgt cctggtatca gcttcggctg gttctggaaa gacttttgta atggttgagc
    31201 ggattttaga taaactttta agaggggttc cgattgactc tttgtttatc tcaacattta
    31261 cagtaaaagc ggcaggtgag ctcaaagaac gtttagaaaa gaaaataaac gagtctttga
    31321 aatccgcaga atcagatgat ttaaaacagt ttctcaccca gcaacttgta ggcattcaaa
    31381 cagctgatat tggtactatg gatgctttta cgcaaaaaat agttaaccaa tatggttata
    31441 ctttgggcat atcacctata tttaggattt tacaagataa aaatgagcaa gacgtcataa
    31501 agaatgaggt ctatgctgat ttattttctg actacatgac tgggaagaat gcagcttcat
    31561 tcataaaatt agtcaaaaat ttctcaggta atcgtaaaga tagcaaagct tttcgagaaa
    31621 tggtttataa agtttatgct tttagccagt ccaccgataa ccccaagcgc tggatgcaaa
    31681 cagtttttct aaaaggtgca caaacttaca ctgattttga agctattcca gatcaagaag
    31741 tttcaagcct tttaaatgta atgcaaacaa ctgctaatca attacgtgat ttaacggatc
    31801 aagaagatta taagcaatta actgctaaag gggttccgac agcaaattac aaaaaacacc
    31861 ttaaaataat tgaaaattta gtccattggt ctcaagactt taatttgctg tatggaaaaa
    31921 aaggattggc taatttggca cgtgatatca ctaatgtgat tccttcaggc aatgatgtta
    31981 cagttgctgg cgttaaatat cctatcttta aacaattgca taatcgtatc gttggactta
    32041 agcacttaga agttattttt aagtatcaag gagaatctct tttcttgtta gaattattgc
    32101 aatcctttgt tctagatttc tcagaacaat atttacagga gaagattcaa gaaaatgctt
    32161 ttgaattttc tgatattgcc cattttgcaa ttcaaatatt ggaagaaaat catgatatta
    32221 ggcaacttta tcaagataag tatcatgaag taatggttga tgagtatcag gacaataacc
    32281 atacccaaga gagaatgtta gaattattgt ctaatggaca taatcgtttc atggttggag
    32341 acataaaaca atcaatttac cgttttcgtc aggctgatcc acaaattttt aatgataaat
    32401 acaaagctta ccaagacaat ccaagtcaag ggaaattaat tattctgaaa gagaattttc
    32461 ggagtcaatc agaggtttta gacagtacaa atagtgtgtt tacacatctt atggatgaag
    32521 aagtaggaga cattctttat gatgaaagtc accaattaaa agctggtagt ccaagacagc
    32581 aagaaagaca tccgaataat aaaacacaag ttttactatt agatactgat gaagatgata
    32641 taaatgacag tgatagtcaa caatatgata ttagcccagc tgaagcaaag ttagtcgcaa
    32701 aagaaattat tcgattgcat aaagaagaga atgttccctt tcaagatatc accctcttag
    32761 tttcttcaag gacaaggaat gatggtatct tacaaacttt tgaccgttat ggtattcctt
    32821 tggtaactga cggtggggag caaaattact taaaatcagt cgaagtgatg gtaatgcttg
    32881 atactttgcg gtcaattgat aatcccttaa atgattatgc tctagttgct cttttgagat
    32941 ctcccatgtt tggctttaat gaagatgatt taacaagaat tgctattcag gatgtcaaaa
    33001 tggcttttta tcataaagtg aaattgtctt atcataaaga aggtcatcat agtgatttaa
    33061 ttactccaga attatcttct aaaatagatc actttatgaa aacattccag acttggcgtg
    33121 attttgctaa atggcattcg ctttatgatt taatatggaa aatatataat gaccgttttt
    33181 attatgatta tgtaggagct ctgcctaaag ctgagcaacg tcaagccaat ctatatgcct
    33241 tggcacttcg agcaaatcag tttgaaaaaa caggtttcaa gggtttatca cgctttattc
    33301 gaatgattga taaagtttta gaaaatgaaa atgatttggc agatgttgag gtggcattac
    33361 cgcaaaatgc tgttaaccta atgactatac acaaatcaaa gggtcttgag tttaagtatg
    33421 tatttatttt aaatattgat aagaaatttt caatggtaga cataacttcc cctcttattt
    33481 taagccgaaa tcaagggatt ggtattaaat atgttgctga tatgcgacat gaactcgaag
    33541 aagagctact gcctgcagta aaggttagca tggaaacact tccttaccaa cttaataaaa
    33601 gagagttacg cttagctact ttatcagagc aaatgcgttt actttacgta gctatgacac
    33661 gtgcagagaa aaaattatat ctggttggta aggctagtca aaccaaatgg gctgatcatt
    33721 atgacttagt atctgaaaat aatcatctcc cattagctag tcgtgagact tttgtgacct
    33781 ttcaagattg gttactggct gttcatgaaa cctataagaa acaagagttg ttttatgaca
    33841 tcaacttcgt atcattagag gaattaacag atcaccacat agggatggtt aatcctagtc
    33901 tacctttcaa tcccgataat aaggcagaga atcgtcagtc tgaagatatc gtgcgcgcga
    33961 ttagcgtgtt agaatctgtt gaacagatta atcaaactta taaagcggct attgagttac
    34021 cgacagtaag aacaccaagt caagtcaaaa aattctatga gcctattcta gatatagaag
    34081 gcgttgatgt gatggaaact attactaaaa catcagtaga ttttaagtta cctgattttt
    34141 ctacaagtaa aaagcaagac ccagcagctt taggatctgc tgtacatgaa ttaatgcaac
    34201 gcatagagat gtcatctcat gtcaaaatgg aagatattca aaaagccttg acagaagtga
    34261 atgctgaaac aagtgtcaaa gcggctattc agattgaaaa aatcaactat tttttccaag
    34321 aaacctcact cggtaagtat atacaagaag aagtagaaca tctgcatcga gaggcacctt
    34381 ttgccatgtt aaaagaagat ccggagagtg gtgagaaatt cgttgtacgt ggtattattg
    34441 atgggtatct actattagaa aatcggataa ttctatttga ttataagact gataaatttg
    34501 tcaatcctct ggaattaaaa gaacgctatc agggacaaat ggctttatat gcagaagctt
    34561 taaaaaaatc ttacgagata gaaaagatag acaaatactt aattctttta ggtggtaaac
    34621 agctagaagt ggtaaaaatg gattaaagaa aggaaaaaat gatagtcgaa caaaaatttg
    34681 gaaatggttt tacttggatt aatattgaag cagagcaatt gcgaacagaa acatcggaaa
    34741 ttcaagcaaa atatttggat agtgagatta ttacatatgc tctggatgat tatgaacgag
    34801 cctttatgga atgttctcat attaaaggaa aagaagtctt gacgattatt tttaatacta
    34861 ttgatctaaa gcagaaagaa agttattatg agaccgttcc gatgaccttt tgcttatcac
    34921 acgaccgttt gataacggtg acacggtctc gaaatagtta tatgctagaa ctattgcaaa
    34981 aatatctaga ccgtaatcca gatgtctctc ctaaaaaatt tttatttgca gctttaactt
    35041 tgattacaaa acaatatttt aatgttgtat cgaaaattga tagagaaaag gatattctca
    35101 accgtcaatt gagagaacaa acaaccaata agcgcttatt agccatgagt gacctagaga
    35161 caggatccgt ttatcttttg acagctgcca atcaaaatgc attggtatta gagcaattag
    35221 atgttcatcc tagtcagcga tttaattctg aagtggaaaa agaacagtta tcagatgctc
    35281 tcattgaagc gcatcagtta gtcagtatga cacaattaaa cagtcaggta ctttcacaat
    35341 tatcatctac ttttaacaat gtcttaaata ataacttgaa cgaaaactta acaggtttga
    35401 atatcatttc gattaattta gcgattattg cagcaattac tggctttttt gggatgaata
    35461 ttccattacc tcttacagaa tcacggagtt catggttaat tgttattgct acttcggtct
    35521 tactttgggt tattattgct caaattttaa aacgagtttt taacttaaga aaataaaaat
    35581 attcggtgcg taagcaccga atatttttat ttacctaagc agaattggct gaagagctga
    35641 gttattagtt catctggagc tgcatcccca gtgatttctc caagtatttc ccaagttcta
    35701 gtcatatcga cttgtaagag atcaacaggc attcctaatt ccaagccctc attgacagct
    35761 tttaaactat caacggcttt ttcaattagt gagatatggc gagcattcga tagataagta
    35821 gcgtcatgtt ctaccatacc agcattgtca aagaaaatat cgttaatacg ttcttcgatt
    35881 ttatcgatgt tttcattttc taaaaccgaa attggaataa cattttcagg taattcgtta
    35941 acttctattt tttgaggaag atccgtttta ttgagtaata cgatacgatt actttcttta
    36001 cttagttcta gaagggagcg gtcttgtaat gttagtggtt cggaactatt gagaaccaac
    36061 aacacaaggt cggcttcttc taaagccttc tttgaacgtt caacaccaat tttttcaacg
    36121 atatcatctg tatcacggat accagcagtg tcaactagtt ttaaaggaac tcctttaata
    36181 ttgacgtatt cttcaataac atcacgagtt gttccttcaa tatctgtcac aatcgccttt
    36241 tcttcacgta agaggttatt tagtaagctg gatttaccga catttggacg accaatgatt
    36301 gctgttgaaa gtccttctcg aagaattttt ccacgtcggg cagttcgaag taaattttcc
    36361 ataagtgctt gaaactcttg tgttttttca cgcatcagtg ttgtagtcat ctcttcaacg
    36421 tcatcatatt cagggtaatc gatattaact tcaacttggg ctagagtatt tagaatttct
    36481 tgtcgagtgt tattgattag tgtctttaga gaaccatcta attgcttaac ggcaatatcc
    36541 attgctttat ctgttttagc tctaataaga tccataacag cttcagcctg agtcaagtca
    36601 actcgaccat ttaaaaaagc acgtttagta aattctcctg gttcagccat tcgtgctcca
    36661 tgtcgtaaaa ttaactgaag aatttcgtta gtaactgcaa ttcctccatg cgtattaatt
    36721 tcaataacat cttctcttgt gaatgttttt ggagcaagca taacgcctaa cataacttca
    36781 tccaagattt catttttatc tgggtccaca atatgaccat agtttaaggt atgggattgg
    36841 atagcggata aatcttttcc acgataaatt tttgatgcta tctttaaggc atctgtacca
    36901 gatatacgga caataccaat agctccctca ccgagaggag ttgaaatagc tgcgatggta
    36961 tcaaattctt ttgtaataga cataaagtct ctctctttct tgggaattac taacattcat
    37021 tgtaacctaa agccctaaaa ggtgcaatca tttgattaaa agagaagatt tccataatgg
    37081 ttttatttga aggtatgcta taataaaact atgattatac tacaaggaaa caaaattgag
    37141 cgctcatttt caggtgatgt gctttttgat aatattaata ttcaagttga tcaacgtgat
    37201 cgtattgctc ttgttggacg taatggtgct ggcaaatcaa cgcttcttaa aattttagtt
    37261 ggtgaagaga ctccgacaaa aggagaaata aataaaaagc gtgatttgag tttgtcctac
    37321 ttggcacaag atagtcgttt tcaatctgaa aatacaattt tccaagaaat gttacaagtt
    37381 tttgactcat taagagaagt agagaaacgc ttacgtgaat tggaattaca aatggggcaa
    37441 gtttcaggaa gtgacctgga acaattgatg aaaacatacg atattttgtc agaagagttt
    37501 cgtgaaaaag gtggctttac ctatgaatca gatataaagg ctattttaaa tggctttaag
    37561 tttaacagtg atatgtggga aatgcctatt agtgaattgt ctggcgggca aaatacacga
    37621 ttagcgcttg caaaaatgct acttgaaaag ccagaattac ttgttttaga tgaaccaacg
    37681 aaccatttag acattgatac gattgcttgg ttagaaaatt atcttgtaaa ttatcaagga
    37741 gcccttatta ttgtcagcca tgaccgctat tttttagaca aggtagctac agtaacttat
    37801 gatttaacga cacattctct agaccgctat gtaggcaatt attcgaaatt tatggatctt
    37861 aaggcagaga aaatagcgac tgaagaaaag aattttgaaa agcaacaaaa agaaattgct
    37921 aagttagaag attttgtaca acgtaacatt gttagggctt caaccactaa acgagcacaa
    37981 gctcgtcgta aacaattaga aaagatggaa cgactcgaca aaccgaatgt agagcaaaaa
    38041 tcagctaata tgacttttca tgctgggaaa gtctcaggga atgttgtttt aacacttgag
    38101 aatgcagcta ttggttatga aggtgtttct ttatcggagc taatcgattt ggacgtgaag
    38161 aaatttgatg ctattgcaat tgtaggtcca aatggtatcg ggaaatcaac tttaatcaaa
    38221 tcgcttgttg ggcaaattcc ttttatcaaa ggagaagcaa aattaggagc aaatgtcgaa
    38281 actgggtatt acgatcaaag tcaaagtaat ttaacaaaaa cgaatactgt tttagatgag
    38341 ttatggaatg ctttttcaac aacgccagaa gttgaaatta gaaaccgttt aggagctttt
    38401 cttttttcag gtgatgatgt taaaaaatta gtttcaatgc tatccggtgg tgaacgagcg
    38461 cgtttgctat tggcaaaatt atctatggaa aataataatt tcctcatatt agatgagcca
    38521 acaaaccact tggatattga tagtaaagaa gtcttagaaa atgcactcat cgaatttgat
    38581 ggcacattac tttttgttag tcacgatcgc tattttatta atcgtgtggc aactaaggtt
    38641 ctcgaaattt ctgataaggg aagtactctc tatctaggag attatgatta ttacctcact
    38701 aagaaagccg aactagagga attagcacgt ttaaatgaag aagaagtgag tgcttcaaaa
    38761 acagaaatcg acgttacatc agattatgag acacaaaaag caaatcaaaa ggaatttagg
    38821 aaaattaccc gtcgcattgc agaaattgag gcgcgtttgg aagttcttga aaataatgaa
    38881 aacaatatca atggtttaat gttagaaacc aatgacatcg gaaaactctc tgacttacaa
    38941 aaagagctgg aatcaatcca agaagaacaa ttgctattaa tggaagaatg ggaaaatcta
    39001 aatatgagac tagatttgtg agaaaatttc aaaaacaata gtttgtgaaa aaaagtacat
    39061 tttaaattga gatgcctttt acgaaaacaa agaaagcggt tcaaattctt gcatcttgtg
    39121 aaaaaaactt atacaataat taagtaataa agattttttg gaggttagaa tggtaactct
    39181 atctaaagaa caacacttgg atatgtttct aaaaatgcaa cgtattcgtg atgttgatat
    39241 gaaatttaac aaattggttc gtcgtggttt cgtccaaggg atgactcact tctctgttgg
    39301 tgaagaagct gcttcagtag gtgctatcca agatttaaca gattcagata tcattttctc
    39361 aaaccaccgt ggacacggac aaactattgc aaaaggtatt gatattggtg gaatgtttgc
    39421 tgaacttgct ggtaaagcaa caggaacatc aaaaggtcgt ggaggctcaa tgcacttagc
    39481 aaaccttgaa aaaggtaact atggaacaaa cggtatcgtc ggtggaggat atgcgcttgc
    39541 agtaggtgct gcccttacac agcaatatga aggaactgat aacattgtta tcgccttttc
    39601 gggtgactca gcgactaacg agggctcatt ccatgagtca gttaacttag ctgctgtttg
    39661 gaacttacca gttatcttct ttatcatcaa caaccgttat ggtatctcaa cagacatcac
    39721 atactcaact aaaattcctc acctttacat gcgtgctgat gcgtacggta ttccagggca
    39781 ttacgttgaa gatggtaatg accttatggc tgtttacgag aaaatgcatg aagttatcaa
    39841 ttacgtgcgt tcaggaaatg gaccagctat tgtagaggtt gaatcatacc gttggtttgg
    39901 tcattcaact gctgatgctg gggtataccg tactaaagaa gaagttgatt catggaaagc
    39961 taaagatcca gtaaaacgct accgtgcata cttaattgaa aatgaaatcg ctactgaaga
    40021 agagcttgca gctattgaag cacaagtgat taaagaagtt gaagaagggg ttaaattcgc
    40081 tgaagaaagt ccattccctg atatgtctgt tgcatttgaa gacgtttttg tggactaaaa
    40141 taacctaaag tagatgagat ttatctcgtt ctattattca attgattaaa aaacggatat
    40201 tcggaggaaa taatgagcga aactaaagtt atggctttgc gcgaagcgat taatgtcgct
    40261 atgagcgaag aaatgcgcaa agatgaaaaa gtatttttaa tgggtgaaga tgtaggtgta
    40321 tatggaggag acttcggtac atcagttggt atgttagaag agtttggtgc taagcgcgtt
    40381 cgcgacacac caatctcaga ggctgcgatt gcaggttcag ctatcggtgc tgcacaaaca
    40441 ggtttgcgtc ctatcgttga tttgacattc atggatttcg taacaatcgc aatggatgct
    40501 atcgttaacc aaggtgctaa aacgaattat atgtttggtg gaggtctatc aactccagtt
    40561 actttccgtg tggcatcagg gtcaggtatt ggttcggctg cacaacactc acaatcatta
    40621 gaagcttggt taacacacat tccaggtcta aaagttgttg cacctggtac tgttaatgag
    40681 tcaaaagcac ttttgaaatc ttctattctt gacaataacc cagttatctt cttagagcca
    40741 aaagctctct atggtaagaa agaagaagtt aacatggatc ctgatttcta tattccactt
    40801 ggtaaagggg atattaagcg tgagggtact gacttaacaa ttgtttctta tggccgtatg
    40861 cttgagcgtg ttatgcaagc tgctgaagaa gttgctgagg aaggtattaa cgttgaggtt
    40921 gttgatccac gtacacttat tccgcttgat aaagaattaa ttatcgattc tgttaagaaa
    40981 actggtaaat tgattttggt taacgatgct tataaaactg gtggtttcac tggtgaaatt
    41041 gcaactatgg ttgctgaaag tgaagcattt gattaccttg atcatcctat cgtgcgtctt
    41101 gcaagtgaag atgttccagt accgtattca cgtgtacttg aacaaggaat cttacctgat
    41161 gtagcaaaaa ttaaagatgc tatctacata gtagttaaca aaggtaaata attcataaaa
    41221 caaagaggga ctgaggaatg agtgaaaact tagcctcttt ccttgtttat aggttttaat
    41281 ttatatcgtt agatgaaaca tatatttgac gatatagttt agtgtgaaag gaaaactatg
    41341 gctgtcgaaa ttattatgcc taaactcggg gttgatatgc aagaaggcga aatccttgag
    41401 tggaaaaaac aggtaggtga tgttgttaat gaaggtgacg tcctacttga aattatgtca
    41461 gataaaacaa atatggagat tgaagcagaa gactcaggtg ttttactaaa aatcacacat
    41521 gggaatggag atgttgttcc tgtaactgag acaattggct atatcggtgc tgaaggtgaa
    41581 gaagtaacag aagcatcttc ttctgaaaat acatcagttg aagaaaacgc gacacaagta
    41641 acttcagaac cagaaaaagt tgaagaaaca tcagaaccaa gtgtgccagc agctacaagt
    41701 ggtgaaaaag tacgtgccac tcctgcagct cgtaaattag caagagagat gtctattgac
    41761 cttgcactag tatctggaac aggggcaaat ggtcgcgttc acagagaaga tgttgaaagc
    41821 ttcaagggtg ctcaaccacg tattacacca ttggcacgtc gaattgctga agatcaagga
    41881 gttgatatag cagaaatcac tggcagtggt attcgtggta aaattgttaa aaatgatgtt
    41941 ttggctgcta tgtcaccgca agcagcagag gctcctgtag aaacgaaagc tacaccaaca
    42001 acagaagaaa aacaattacc agaaggtgtt gaagtcatta agatgtctgc aatgcgtaag
    42061 gctatttcta aaggtatgac taactcttac ctcactgctc caagctttac acttaactat
    42121 gacattgata tgactgaaat gatggctctt cgcaagaaat taattgatcc aatcatggct
    42181 aaaacaggct tgaaagtcag ctttactgat ttgattggaa tggccgttgt taagacattg
    42241 atgaaaccag agcatcgata cctaaatgct tcattaatta acgatgcaca agaaattgag
    42301 cttcataatt ttgttaatat tggaatcgca gttggtcttg atgatggctt gattgttcct
    42361 gttgttcaca atgctgatca aatgtcactt tctgactttg ttatagcatc taaagatgtt
    42421 attaagaaaa ctcaagaagg taaattaaaa tcagctgaaa tgtcaggttc aacattctca
    42481 attactaact taggtatgtt tggtactaag acgtttaacc caattatcaa tcaaccgaac
    42541 tcagcaatcc ttggagtagg cgctactatt ccgactccaa cagtggttga tggtgaaatt
    42601 gtagctcgac caatcatggc aatgtgttta acaattgacc accgtattgt tgatggaatg
    42661 aatggtgcta agtttatggt tgatcttaag aacttaatgg aaaacccatt tggtttactt
    42721 atttgattcc gtaggtctta atttgatata ataagttatt cgaaaaacga aagaggaaat
    42781 aaaagatggc ttttgatgta attatgccaa aacttggggt tgacatgcaa gaaggcgaaa
    42841 tccttgagtg gaaaaaaaat gaaggtgaca ccgttaatga aggtgatgtc cttcttgaaa
    42901 tcatgtctga taaaaccaat atggaaattg aagcagaaga cactggtgta cttttaaaaa
    42961 ttgtacatca ggcaggagat gttgttcccg tcactgaagt gattgcctat atcggagaag
    43021 aaggtgaaga agtaggtacg tcatcacctt cagctgatgc aactatcaca gctgaagatg
    43081 gtcaatcagt ttctggacct gctgctcctt cacaagagac agttgcagca gcaactccta
    43141 aagaagaact tgcagctgat gagtacgata tcgtagtcgt aggtggtgga cctgcaggtt
    43201 attacgcagc aatccgcggt gctcaacttg gaggaaaaat tgccattgtt gagaaaactg
    43261 aatttggtgg tacgtgtttg aatgttggtt gtatccctac aaaaacatat cttaaaaatg
    43321 ctgaaatcct agatggtttg aaagtagcag ctggacgtgg tatcaacctt gcttcaacta
    43381 actatgcaat tgatatggat aagactgttg cctttaaaaa ttctgtagtt aagacactga
    43441 ctggcggtgt acgtggtctc ttgaaagcga acaaagttga aattttcaat ggacttggac
    43501 aagtaaaccc tgataagtct gttgttattg gcgataaagt tatcaaaggt cgtaacgtcg
    43561 ttcttgcgac tggttctaag gtatctcgta ttaatattcc aggtattgaa tcaccattag
    43621 tattaacatc agatgatatt cttgatttac gtgaaattcc taagtcactt gctgttatgg
    43681 gtggtggtgt cgtaggtatt gagcttggtc ttgtatgggc ttcatatggt gttgacgtaa
    43741 ctgttattga aatggctgat cgcatcattc cagcaatgga taaagaagta tcgctagaac
    43801 ttcaaaaaat ccttgctaag aagggaatga agattaaaac atcagttggt gtttctgaaa
    43861 ttgttgaagc caataatcaa ttaacgctta aattaaacaa cggcgaagaa gttgttgctg
    43921 ataaagctct tctttcaatt ggacgtgttc cacaaatgaa tggtcttgaa aatcttgagc
    43981 ctgaacttga gatggaacgt ggacgtatta aagttaatgc ttatcaagaa acttcaattc
    44041 caggtatcta tgcaccaggt gatgttaatg gaactcgtat gttagctcat gctgcatacc
    44101 gtatgggtga agtagctgct gaaaacgcac tcggcggtaa taagcgtaaa gcacatctag
    44161 atttcactcc agcagctgtt tatactcatc ctgaagtagc aatggtgggg atgacagaag
    44221 agcaagcacg tgaacaatat ggtgacatat tagttggtaa aaatagcttt acaggcaatg
    44281 gtcgtgctat cgcttcaaac gaagcacatg gttttgtaaa agtaattgct gaacctaagt
    44341 ataaagagat tcttggtgtt cacattattg gtccagctgc agcggaattg attaatgaag
    44401 cttctacgat tatggaaaat gagttgactg tctatgatgt agcacaatct atccatggtc
    44461 acccaacatt ctctgaagtt atgtacgaag ctttcttaga tgttctcggt gaagctattc
    44521 acaaccctcc aaaacgcaaa taattttgtt taatatattt gagattgagc ttttatagct
    44581 tcaatctctt ttcacgtata tattatctga atattataaa atattagtta ggaatttaaa
    44641 atgaaatata tcgttaacac gtctaatgat ccggcttaca atgttgcttt agaagcttac
    44701 gcctttcaaa agttgacaga tatcgatgaa atttttattc tttggattaa tgaaccggct
    44761 atcattattg gacgccatca aaatacaatt caagaaatta acaaagagtt cattgataaa
    44821 aatggcatcc atgttgtccg tcgtttgtca ggaggcggag cagtttatca tgacttgaat
    44881 aatttaaact acactattat ttctaataat acacaagaag gtgcctttga tttccaaact
    44941 ttttcaaaac cagttattga tactcttgct aaattaggtg ttaaagcaga atttactggt
    45001 cgtaatgatt tagaaatcaa tggacaaaaa tttgcaggaa atgctcaagc ttattacaaa
    45061 ggtcgtatga tgcatcatgg ttgtctttta tttgatgttg atatgtcagt cttagggcaa
    45121 gcactcaaag tatctaaaga caaaattgaa tctaaaggga taaaatctgt acgcgcacgt
    45181 gttacaaata ttgttgacca tctttcagat aaaattacag tacaagaatt tagcgatgcc
    45241 attttagctc aaatgaagga agaatatcca gagatggatg aatatgtttt gagtgatgcc
    45301 gagttatcag aaatccaagc tatgcgcgat aatcaatttg ctacatggga ttggacgtat
    45361 ggtaaagcac ctgaatacac cattgaacgt ggtgtccgtt atccagctgg aaaaattacg
    45421 acttatgcta atgttgaaaa ttcaacgata aaatctgtca aaatctttgg tgacttcttt
    45481 ggagtgaaac ctgtagatga tattgaaaaa atgttggagg gtgtccgcta cgattacaaa
    45541 gatgttttag cagcgcttaa aacagttgat acaagtcaat acttctcacg tatgacaccc
    45601 gaagagatta caaaagctat tgtagactaa aatcaaaaaa tctagtcaaa gtctttgcct
    45661 ttggctaggt ttttgtatgg ttaagtggcc ttatagtagt aaaaaaccaa cgttattgat
    45721 aacgctggtt tttatattat tcaaaatctg cttttgattt aacgtcgccg tattcctcag
    45781 gaatctctaa agataaaatt tcttcataac tagggaggac aatctcttta ccgtatttgt
    45841 tacggagggc agttgccaca agacggtaag ctagattagc attacgagaa aggattggac
    45901 cgtgaaagta tgacccaaaa acattcttat aatgaacccc ttcagtatca tcttctttgt
    45961 tattcccatt tccataaatg acagctccta aaggtttttc atcttctgat aaaaaggtac
    46021 gtccttgatg attctcaaag ccataatatg tttcattgaa ttcatcgttg tgaatcttga
    46081 tatcaccaat atagcgatta ttattttggt ttagcgtata gtgtcccata acgccaatgc
    46141 cctctattcg ttctccatta gcttgaatat agtattgtcc taaaagttgg aaacctccac
    46201 aaatagcaag tactacacca ttattttgaa tgaatttgtt aatgtcttct ttcttactgg
    46261 gaagatcgcg tgcaacaatg gcttgttcgt agtcttgtcc accaccaaaa aatgccatct
    46321 gataatagtt gggatcaaat ctatcttcta atgaaacgat atcaaatgtc atttgacatc
    46381 ctagtttttc tcccacatat ttcatcatta agatattccc attatcgcca taagtattta
    46441 ataggttacc ataaagatga gctacattaa gagtgtattt ataatctttg gtagtaggag
    46501 attttagtga ggtataagtc attaattcat ctcctttctg atagcgttgt ggttagcaag
    46561 aatttcacga aactccagca tagctgtata tgttgctaaa atataagcat gctcagtctc
    46621 ttgctgctca atcattgcca taatatcttg cagtttatca gcttgtttta tgcgtttctc
    46681 atcataacca gttacacgta agcgacgagc aatttcagag tggcgaacac caccagcaaa
    46741 tatttctggg atattcatcg tgagaatagt ttcaaaatta gcgtcccaaa tccaagaagt
    46801 atcaataccg tcagcataat tggcattcag tagtactgag agagagaacg ggtatggagc
    46861 aagtttaatc atatcaagag cttgactagc tcctaccgga tttttaatca gaactaaagt
    46921 acatttttta tttccaattg taaaggtttc ttgacgacca aaaacggcac ggcttttatc
    46981 aaatccatcc ttaatttgac ttggttctac accaaaatat tcagcaacag atactgccgc
    47041 caaagcattg taaatattat aaaggccacc cacgttgata ttatattgtt ggccatcaat
    47101 aacgaaacct gatgaagtat ttgtcagatg tgttagcctt gttaaggcat aatcaaggtt
    47161 tggacgttca aaatcacaat taggacaagt gtagtcacca aggttggcat atgtgttcaa
    47221 tcgataagtt aaaattgctt gacactttgg acagagaata ccctcagtat tatagtgtgc
    47281 taatcgtggc tcatgtttgt cggtatcaaa tccatagaat tgcacagggt tagtcacgct
    47341 tttagaatta aaaagtggac tgtcaccgtt agctaagata gtagcttgcg gagcgttagc
    47401 agctccatct aaaatcattt gataagttgt gtagatttcg ccataacggt ccatttgatc
    47461 acggaaaata ttggtaaaga cgaacaaact aggctttata tactgtgtaa tacggggtaa
    47521 actcgcttcg tctatttcaa gaacagctat ttttttacca gatttacttt tcttagctgt
    47581 taggaaggtc gaaacaatac ctgtaatcat atttgctcca cttgggttgg ttaccacctg
    47641 accgaaggct tcctttaaaa taccaactgt taaagctgtt gttaaggttt taccgttagt
    47701 accagtcaca actacaattt cataatcttt ggcaattgta tctagtatat ctttatcgaa
    47761 tttgagtgct aaactacccg gataagtaga gccacgtccc attttactta aaaggtaatg
    47821 agcagactta ccggcagcca ctcctagtgc tgtatttatt ttcataaaga tattttatca
    47881 taaaaagatg ctttcttgct aaagaaaata ttatgaaaac atggtataat gtagacgata
    47941 gaaaataaga aatcaaggag ttaagcaaga aactcttcga caggctacca gtagagagga
    48001 agatatggat attttttcag ctattgattc aaagttttgg gcgagcatta tggaaaatcc
    48061 atggatgatc cttattcatc tcatggatat tatcatagta gctgttctga tttataaatt
    48121 tatcaaagcg ctagcaggca caaaaattat gtccttaatt cagggcgtta ttttgtttat
    48181 catcattaga tttgtgtcag aatggatagg gctaacaacg attacatttt tgatgaatca
    48241 gattgttact tatggtgtaa ttgcaggtgt tgtcatcttt gctccagaga ttagaacagg
    48301 tctcgaaaaa tttgggcgaa caccacaact atttacgcaa cgttctcaat taagcagtga
    48361 tgaaaagcta gtagatgccc ttgttaaggc agttgcttat atgagtccgc gtaaaatagg
    48421 agccttgatt tcaattgaac gtacccaaac tttgcaagag tatatagcta caggtatccc
    48481 attagatgca gatatttcca gtgaattgtt aattaatatt ttcattccta atacaccact
    48541 gcatgatggt gctgttattg taaaagataa aaaaattgct acagcatgtt cgtacttacc
    48601 tctatcagaa tcatcttcga tttcaaaaga atttggaaca cgccacagag cagcgattgg
    48661 tctatcagag aattcagatg cactaacagt tattgtttct gaagagactg gtggtatttc
    48721 tgtcgctttg aaaggtgaat ttttacatga tctttctaaa gatagctttg aagcaattct
    48781 gagaactcag ttgattcaaa atcaagagga aaactcaaaa ttagcttggt ataatcaact
    48841 attgaggagg aagtagacat gaaaaaattt tttacaaata aattttggtt aggcgttgtc
    48901 tcactctttc tggctatttt attattttta acggcaactg caacaagtat gaaccatcaa
    48961 gacaatagta agattgcagg agcgtctgag acatatacac ataccctaac agatgtgcca
    49021 atagacatca aatacgatag tgatgattac tttattagtg gatattctta tggggcagat
    49081 gtctatatgt catcggttaa tcgtgttaag ttagattctg aaataaatga ggatacacga
    49141 aaatttaaag tagtcgctga tttgacaaat atgaaaccag ggactcacaa agtgccacta
    49201 aaggtagtaa atttgccatc tggtgtaaat gctacggttt ctccaacaac aattacagta
    49261 actatgggta aaaagaaaac aaaagagttt ccggtttatg gacatgttaa tgataagcaa
    49321 attaaagctg gttatgcagt cgataagatg tcagttgatg tgtcaaaagt taaagttact
    49381 agtgatgaat ctattataga tcgtattgac catgtcgctg ccaatatccc tgatgataaa
    49441 gttcttgatg atgattttaa caagacagtt actttacaag ctgtaacagc agatggtaca
    49501 gtattagcaa gtattattca cccttctaaa gcaactttat ctgtcaaagt aaaaaaattg
    49561 accaagacag taccgattaa tttgatacca gtaggacaat tcagcgatag tatttcaaaa
    49621 attaactaca agttgagtca agaaaaagca gtaatttcag gaacaaaaga ggctttagaa
    49681 gctatttctg ttattaatgc tgaggtagat atttcagacg taactaaaaa tacagaaaag
    49741 aaaattaatc tttctgcaaa caatgtttca gttgatcctg ctcaagtaac agttcaattg
    49801 acaactacta aaaaataata gataataaaa acgaaaaaaa tggaggtagc gtttttcgct
    49861 agaaaaaaat tatgggaaaa tattttggta cagatggtgt tcgcggtgaa gctaatgttg
    49921 aactgacgcc agaattggct tttaaattag gacgttttgg aggatatgtt ctaagtcaac
    49981 acgaaactga tcgtccgaga gtttttgtag ctcgtgatac tcgtatttct ggtgaaatgc
    50041 tggaatctgc tttgattgct ggtctacttt cagtaggtat tgaggtttat aaacttggtg
    50101 ttttagccac tcctggagtg tcttaccttg tccgtacaga aaaagctagt gcaggggtta
    50161 tgatttcagc aagccacaat ccagcacttg ataatggtat taagttcttt ggtagtgatg
    50221 gttttaaact tgatgatgat cgtgaattag aaattgaagc tttacttgat gcgaaggaag
    50281 acactttacc acgtccatca gcacaaggtt taggtacttt agtagattat cctgaaggac
    50341 ttcggaaata tgagaagttt atggaatcaa ctggtattga tttagaaggt atgaaggttg
    50401 cgttggatac agcaaatgga gcagcaacag cctcagctcg gaatatcttt ttagacttga
    50461 atgctgatat aagtgttatt ggtgatcaac cagatggttt gaatatcaat gatggtgttg
    50521 gttcgacaca tcctgagcaa ttacaaagtt tagttagaga aaatggttcc gatatcggtc
    50581 tagcttttga tggtgatagt gatcgtttga tcgctgttga tgaaaatgga gagattgtag
    50641 atggtgataa aattatgttt atcattggga aatatctttc tgataaaggg cagcttgctc
    50701 agaatacaat cgtaacaacg gttatgtcaa atctcggctt tcataaagcg ctcgaccgtg
    50761 aaggtattca taaagcgatt accgctgtag gagaccgcta cgtggttgaa gaaatgcgta
    50821 aatcaggata taatcttggc ggggagcaat caggccacgt tattatcatg gattacaata
    50881 ctacagggga tggacaatta acagccatcc aattgactaa ggtcatgaaa gaaactggta
    50941 agaaattatc agagttagct agtgaagtga cgatttatcc tcaaaaatta gtaaatattc
    51001 gtgttgaaaa taacatgaaa gataaagcga tggaagttcc tgcaattgcg gaaattattg
    51061 ctaaaatgga agaagagatg gatggcaatg gtcgtattct agtacgtcct agtggtactg
    51121 agcctcttct tagagttatg gcagaagcgc caacaaatga agcagttgat tattatgttg
    51181 atactatcgc agatgtcgtt cgtacagaaa ttggtttaga ttaaaacggc gttattctgc
    51241 tataaagtcg tcaatgttat aaaataataa agagagggaa ttggagtgga gcatcaagca
    51301 tacgcctttc tctctctttt tgatttgaga agggagttta gagaaaatga ctaaagaagc
    51361 aaaattatta tgggctaaac acaagtattt agtcttaagt aaatctcaga aaatttattt
    51421 ggatattaga caaactttga aatcaccaaa ttgtaccgtt ttagacgttc agtcactcat
    51481 tgaccaagca gtgcttttag aagaatcacc atcacaagtg actaatgctt atatgcatat
    51541 atggggctat tttaaaaata aggcagagcg tcaagaaaaa gaagaatttt taaccttgct
    51601 ggaaaaatac aggaaaacag gttatcaacg aagaaaactg ttagcttttc taaaacagct
    51661 attagctaaa tacccaaatt cttatttaca aaactcttct attttcgaag aagagtaaaa
    51721 gattgatagt tgaggtatat aaatgagatt atggcatcaa gatattattg agttattgcc
    51781 taggcagcaa cttttaggac agcatagaga gtgttgtgca ttgagaggga atgggtgggg
    51841 gagaaaacat gagactgttg attatgtttt tcgatattca ccttataggt tatttgcata
    51901 tcatcaatta gttatggaag aaatgatgga gcgtgggtat cgtgtatcta aagaatggct
    51961 aattgcagaa tataggggaa tgaagtgccc aagatatgat acacttaatc ctgttgactt
    52021 ggagacacct atctatcctg agcacaatca agactatcta caagaatgtc tatggaattt
    52081 aaaagcaaaa ggaattgacc ttcctataaa taagataaaa tagagattta tctctatttt
    52141 attttggaat aaaaagctat attatcaagt tttagaggag tgttttttta agggcaaagt
    52201 ggtaaaatag aaacatgtta aagaaaccaa cctcagcata tgtacacatt ccattttgta
    52261 cccaaatatg ttattattgt gatttttcaa aagtttttat taaaaatcag cctgtagacg
    52321 cttatcttca ggctcttatc cgtgaatttc ggtcttatga tattacagaa ttgcgcacct
    52381 tatatattgg aggagggaca cctacatcta taagcgctgt gcaactggac tatttattga
    52441 cggagttaat tagagattta aacctaaata ctttagagga gttcactatc gaagctaatc
    52501 caggagattt aaccgttgat aaaatagagg tgcttcaaaa gtcagctgtc aatagagttt
    52561 ctctaggagt tcagaccttt aatgataaac atttaaagcg tattggacgc agtcacaatg
    52621 aggcacaaat ttattctaca attgatgctc ttaaaacagc cggttttcaa aatatctcta
    52681 ttgacctaat ttatgcctta cctggacaaa cgatggatga tgttagaagt aatgtagcca
    52741 aagctctctc tctaaatatt cctcatttga gtttatatag tttaatttta gaacaccata
    52801 ctgtatttat gaataaaatg cgtcgtggga aattacattt gccaacagaa gatttagaag
    52861 ccgagatgtt tgaatatatc atttctgaaa tggaaagaaa tggttttgag cattatgaaa
    52921 tttctaattt tacaaagccg ggttttgaaa gtcgtcataa tttgatgtac tgggataatg
    52981 tagaatatta tggtgttggt gcaggtgctt ctggttatct tgatggtatt cgttatcgta
    53041 atagagggcc aatacaacat tatctaaagg gtgtttcaga aggtaatgca aggttatctg
    53101 aagaagtatt atcaaaaaat gaaatgatgg aagaggagct tttcctcggt cttcgtaaaa
    53161 aagagggtgt ttccatcgga aaatttgaac aaaaatttgg aacgtccttt gagaaacgtt
    53221 acggtcagat tgttcaagaa ctccaaagtg atgggctttt gaaagaaaat aatggtttta
    53281 tccagatgac taaaaaaggt ctcttcttag gagatacggt tgccgagaaa tttattgtgg
    53341 agtaaaaaat gggtttatta tatcgagaaa cttacgaagt gcctttttat gaatcagaca
    53401 ctaatcatta tatgaaatta ccgcagttgt tggctttagc gttacaaatt tcagccaaac
    53461 aatctttaaa attaggaata ggcgatgaca tcgtttttaa aagatacgga ttggtttggg
    53521 tcgttacaga ttatattatt gatattgagc gtttacccaa gcatgctgaa aagatagtca
    53581 ttgagactga agccaaagct cataataaat tattgtgtta ccgctatttt tatatttatg
    53641 gtgaagacgg gcaaaaaata ataacgattt catctgcttt tgtcttaatg gattttaaaa
    53701 cacggaagat tcatcctgtt cttgatgata ttacttcaat ttaccaatcc caaagaatca
    53761 aaaaagttat tcgagggccg aaatatcatc ccattggaga tagtaaggtt aaacaatacc
    53821 atgttcgtta ttttgattta gatatgaatg gacatgttaa taatagtaag tacctagaat
    53881 ggatgtatga tgtgttggac ttagattttc taagtagcca cattcctaaa aaaattgatt
    53941 taaaatatat taaggaaatc caatatggta cggatattaa gtctcattgg tatcaagatg
    54001 gtcttgtgac acgtcatgat attattggtg gcgatgctat tcatgctcaa gctaggattg
    54061 aatggcagga aaaaaaggag gattaaatgg cttataaagg atatcttatt gatttagatg
    54121 gtactattta caaagggaaa agtcgtatcc cagcaggaga gcgttttatc gaacgcttgc
    54181 aagaaaaagg cattccttat atgttagtaa ctaataacac gactcgcaca ccagaaagcg
    54241 ttcaggaaat gcttcgaggt ttcaatgttg aaacaccact tgaaacaatc tataccgcaa
    54301 ctatggctac ggttgactat atgaatgata tgaaccgtgg aaaaacagct tacgttattg
    54361 gtgaagaagg gcttaaaaaa gctattgcag atgcaggtta tgtggaggat acaaaaaatc
    54421 cagcctatgt ggttgttggt ttagattgga atgttactta tgataaattg gcaactgcta
    54481 cgttagcaat tcaaaatggt gctttattta ttggaaccaa tccagatctg aatattccta
    54541 ctgaacgtgg tttgttaccc ggtgctggct ccttaaatgc tttattagaa gccgcaacgc
    54601 gtataaaacc agtctttatt ggaaagccaa atgccatcat tatgaataag gcacttgaaa
    54661 ttttaaatat ccctaggaat caagcagtca tggtcggtga taattatttg acagatatca
    54721 tggctggtat taataatgat attgatactt tgttagtaac aacgggtttt acaacagttg
    54781 aagaagtacc agatttacca attcaaccaa gttatgtttt agcaagttta gatgaatgga
    54841 catttaatga aggataagtt attaatagtt ttaacttgga tttggattat atcgttagca
    54901 actctagcta caatttatat tgcttggttg atctatccta tagaaattca gtttttaaaa
    54961 ttagaaaagg ttgtgtattt aaaggctgaa acaatttatt ataattttaa taagttgatg
    55021 atatatctga ctcatccttt tatctctgat ttaaatatgc ctagctttcc ttcttcagag
    55081 gatggtttaa agcattttgc ggatgtaaaa tatcttttta ctctagcgca tgggcttttt
    55141 gttatactaa cttttccagt tatttatttt ttaagacgtg gatggaagca aaaaagtatc
    55201 tttctatatg aaggcttctt caagatagct attatgttgc ctatttttat tgtagtatgt
    55261 gcttttctgt tgggttttga tcagtttttc actctatttc atgaggtgct ttttccagga
    55321 gattctactt ggcaatttaa tcctctaacg gatccagtaa tttggatact tcctgaaact
    55381 tttttcctcc attgtttcat catttttttg ttaatctatg aaacaataac tataattctt
    55441 ttgataatag gtagaaaaca tttaaaatta agaaaatata aaaacaaaat gcaa
//
LOCUS       DATRTN010000007        53717 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.007, whole genome shotgun sequence.
ACCESSION   DATRTN010000007 DATRTN010000000
VERSION     DATRTN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53717
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.007"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(29..202)
                     /gene="secE"
                     /locus_tag="VBE22_000405"
     CDS             complement(29..202)
                     /gene="secE"
                     /locus_tag="VBE22_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEO7201883.1"
                     /translation="MFVKGIFQVLRDTTWPNRKQRWKDFISILEYTVFFTIVIYIFDK
                     LLAAGVMDLINRF"
     gene            complement(238..390)
                     /gene="rpmG"
                     /locus_tag="VBE22_000406"
     CDS             complement(238..390)
                     /gene="rpmG"
                     /locus_tag="VBE22_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO7201884.1"
                     /translation="MAQKKASLACTECGSRNYSIGVSSTPKPTRLEVNKFCKYCKKYT
                     IHKETR"
     gene            complement(439..2760)
                     /locus_tag="VBE22_000407"
     CDS             complement(439..2760)
                     /locus_tag="VBE22_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7201885.1"
                     /translation="MKLFDKFIDLFRVDEDNDEMTRKNEQETREETSNLDGEEVYDID
                     DITRPSKSQYQRGIRHQKENAKSRPEWLQKVDRYLPSPKNPIRRFWRRYRIGKLLFIA
                     LMAFILIFGSYLFYLSKTATVSDLQSALKTTTTIYDKNKEYAGKLSGQKGTYVELNAI
                     SDHLKNAVIATEDRTFYENNGVNFKRFFLAVATLGKFGGGSTITQQLAKNAYLSQDQT
                     IKRKAREFFLALELTKKYSKAEILTMYLNNSYFGNGVWGVEDASRKYFGTSAANLTVD
                     EAATLAGMLKGPEVYNPYYSVENATNRRDTVLAAMVDAGKLTKSQAKEAASIGMKNRL
                     ADTYAGKINDYRYPSYFDAVVNEAIDTYGISEKDIVNNGYKIYTALDQNYQSGMQKTF
                     DDTSLFPVSDYDGQSAQGASVALDPKTGGVRGLVGRVQSTKDAQFRSFNYATQSKRSP
                     ASTIKPLVVYSPAIASGWSIDKELPNKVQDFHGYKPSNYGGIETESIPMYQALANSYN
                     IPAVYTLDKLGINKAFTYGRKFGLNMSSANKELGVALGGSVTTNPLEMAQAYSTFAND
                     GIMHRAHLITRIETANGKLVKQFTDKPKRVISRSVASKMTSMMLGTFSNGTAINANVY
                     GYTMAGKTGTTETDFNPNLSGDQWVVGYTPDVVISQWVGFKNTDKHHYLTDSSAGTAS
                     NIFSTQASYILPYTKGSSFTHIENAYFQNGIGSVYNAQDASNTTNQESRSIINDLKDS
                     ASKAAQDISRAVEDSNFQEKVKDAWNSLKDYFR"
     gene            2804..3688
                     /locus_tag="VBE22_000408"
     CDS             2804..3688
                     /locus_tag="VBE22_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO7201886.1"
                     /translation="MSFSLKIRNPYGEHTVKELLEDYFLIPRKIRHFLRVKKHVLINN
                     EFINWQTVVQENDTITLIFDDEDYPTKKIPLGRAELIDCLYEDEHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSAYSGQTCYVVHRLDMETSGAVLFAKNPFILPLINQRLERKEIWR
                     EYWALVEGKFSPKHQVLRDKIGRNRHDRRKRIIDSKNGQHAMTIIDVLKYIQNSSLIK
                     CRLETGRTHQIRVHLSHHGHPLIGDPLYNPSSNNERLMLHAHRLTLSHPLTCETISVE
                     APSSTFEKILNNYKKGVG"
     gene            complement(3713..5353)
                     /locus_tag="VBE22_000409"
     CDS             complement(3713..5353)
                     /locus_tag="VBE22_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201887.1"
                     /translation="MFDFFKKKVVKVCLVIFGIVLVSLLSLGFFYFSKGQVLSRFVAA
                     RSRTSGQAFDNIKEYMVWSDTGESITNDEANYANFEPLSKSEARKLGQEIKEGNKNDS
                     MYLKRVGSRLGIFPDYRIANKPMSLTLKTNVPKLDVLLNQKKVATSNSDHFSVTVERL
                     PRTHYTASLEGTSDGKEIKLKKDYDGKNQTIDLSVAFKSFTVTSNLMDGNLYFGDNRI
                     AKLKDGSHSVENYPVTDGSKAYIKKVFNDGEITSHKQKLISIADNQTIKLDVDGLLNE
                     KEAGQKLITAFNQLILYVSTGQDPQTLGTVFEKGAENDFYKGLKESIKAKFVTDNRKA
                     SHFTIPNIVLNKMTQVGKESYQVNFAADYDFNYDKSTDPDKKTYGHIIQNLTGNFIMK
                     KSGNSYLISNDGKKDITVAKETNKVKADPVSIFPENLVGSWKGEVEDGTVTMTFDKDG
                     KVTQKKVYKDSKSKESNHSAKVTKLEDKGNGLYLYQYESGTDTTTFVTGGIGGLKVKY
                     AYGIKIEGNKIIPVIWQTSSDGEFDYHKPLLSKPLTKQ"
     gene            complement(5523..6734)
                     /locus_tag="VBE22_000410"
     CDS             complement(5523..6734)
                     /locus_tag="VBE22_000410"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO7201888.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIISAMRDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
     gene            complement(6801..7472)
                     /gene="deoC"
                     /locus_tag="VBE22_000411"
     CDS             complement(6801..7472)
                     /gene="deoC"
                     /locus_tag="VBE22_000411"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEO7201889.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAEKFIALGASRLGTSRIIKIVKNQKV
                     EEGTY"
     gene            complement(7502..8704)
                     /locus_tag="VBE22_000412"
     CDS             complement(7502..8704)
                     /locus_tag="VBE22_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEO7201890.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQVVSVVSTGVTKVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGDLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRGTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            complement(8725..9504)
                     /gene="udp"
                     /locus_tag="VBE22_000413"
     CDS             complement(8725..9504)
                     /gene="udp"
                     /locus_tag="VBE22_000413"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEO7201891.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            9662..10399
                     /locus_tag="VBE22_000414"
     CDS             9662..10399
                     /locus_tag="VBE22_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7201892.1"
                     /translation="MPKNELNNKLNKLKHVQVYNKIFNMIQDGTYSPGMQLPSEPELA
                     GQLNVSRATLRKSLALLQEDHLVKNIRGKGNFIRENSSNLSENGYENRQHPIKTCLTS
                     KITEVELEFRVEVPAEAITASLKQETPVVVIADRWYHTDDGPLAYTLSFIPIELISDA
                     EISLHDTKQLLNFIEEGIYQEGISSHSQSHLGYATSGNFSATKYTLSDHGQFILIQET
                     IFKQEKILMCNKHYVPIEHFELSITSH"
     gene            10412..10776
                     /locus_tag="VBE22_000415"
                     /pseudo
     CDS             10412..10776
                     /locus_tag="VBE22_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10808..12430)
                     /gene="groL"
                     /locus_tag="VBE22_000416"
     CDS             complement(10808..12430)
                     /gene="groL"
                     /locus_tag="VBE22_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEO7201893.1"
                     /translation="MAKDIKFSADARSAMVRGVDILADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIETAVSAAVEELKEIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVSELENPYI
                     LITDKKISNIQEILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVVTEDLGLDLKDATMQVLGQSAKVTVDKDSTVIVEGA
                     GDSSAIANRVAIIKSQMEATTSDFDREKLQERLAKLAGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVSGGGTALVNVIEKVAALKLNGDEETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIERLKQSEIGTGFNAANGEWVDMVTTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPEAPTAPAMDPSMMGGF"
     gene            complement(12526..12810)
                     /gene="groES"
                     /locus_tag="VBE22_000417"
     CDS             complement(12526..12810)
                     /gene="groES"
                     /locus_tag="VBE22_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEO7201894.1"
                     /translation="MLKPLGDRVIISFVETEEKSVGGFVLAGASHDATKTAKVLAVGD
                     GIRTLTGELVAPSVAEGDTVLVENGAGLEVKDGNEKVTVVRESDIVAVVK"
     gene            complement(12985..13788)
                     /locus_tag="VBE22_000418"
     CDS             complement(12985..13788)
                     /locus_tag="VBE22_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7201895.1"
                     /translation="MSLLELVNLHKTFEKGTVNENHVLRGLDLTIEDGDFISVIGGNG
                     AGKSTLLNCIAGLIPIDQGAITLDNQSITKDSVEKRSKDISRVFQDPRMGTATNLTIE
                     ENMAIAHKRGNKRHIFRQSVTDDDRQLFKKSLSQLGLGLENRMKTDAAFLSGGQRQAL
                     TLAMATLVRPKLLLLDEHTAALDPKTSDMVMELTQKVIEEQRLTALMITHNMEHAIAY
                     GNRLVMLYHGKIVVDVKGEAKRNLTVAELMELFHKNSGQQLIDDALVLG"
     gene            complement(13793..15667)
                     /locus_tag="VBE22_000419"
                     /pseudo
     CDS             complement(13793..15667)
                     /locus_tag="VBE22_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364177.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(16132..16929)
                     /locus_tag="VBE22_000420"
     CDS             complement(16132..16929)
                     /locus_tag="VBE22_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7201896.1"
                     /translation="MVRLIFSDIDGTLINSNFKVTPKTRQGIKQIVAQGATFVPISAR
                     MPEAITPIMEQIGIDSYIISYNGALIQDMQQKTIASHTMDGQVALQVCSYVSKHYSKI
                     AWNVYRYHEWYSCDKENEWVQKEEEIVGLQSKEMSLMELEKQDRIHKLLLMGEPSLMG
                     ELENTLKAQYPHLSIAQSAPYFIEIMAPGIEKGKSAKTLADYLDISLADSIAFGDNYN
                     DLNLLEIVGKGFVMGNAPKDLQERIGNVTQDNDNDGIYYALVEYMEN"
     gene            17115..17975
                     /locus_tag="VBE22_000421"
     CDS             17115..17975
                     /locus_tag="VBE22_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7201897.1"
                     /translation="MSYPYKANHSIESITLKVNDLENLVNFYSDIIGLTVIDKSSTRA
                     LLGVNQKIPLIILEKTELEKHSTYGLYHTAILVPDEYHLSLALNHLLSQHIPLEGGAD
                     HGYSNAIYLSDPEGNGIEIYNDKDISMWDIRESGQIIGITERLDIDNLLDSLVNVPNN
                     YKLSEKTSIGHIHLSVKDAKISSKLYQNVFGLDEKFAIPTASWIASGNYHHHLAFNNW
                     AGPNLSKNQEDRPGISLLTIAYNDDNLFRDSLKKAQLYQLTFLEKQDHYYIIEDFDGI
                     RIKVVLKPEN"
     gene            18018..18749
                     /gene="yaaA"
                     /locus_tag="VBE22_000422"
     CDS             18018..18749
                     /gene="yaaA"
                     /locus_tag="VBE22_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEO7201898.1"
                     /translation="MIKILIPTAKEMKVCQNIAWPKLSAQTKIIIDYFSTLTVSDLED
                     IYRINTSAARCEAQRWQDFKAKQLTLNPAIKLFNSLMYRNIKRHNLSTSEAQFMENSV
                     FITSALYGIIPAMTLISPHRLDFNTKIKINNNSLKVFWRENYDTFMQSDDIMVSLLSN
                     EFETVFSPKERQKLIHLNFIEDRDGQLKTHSTISKKARGKCLTAMMENNCQTLEHLKQ
                     LRFDGFCYDNELSDSKQLTFVKKQT"
     gene            complement(19106..19723)
                     /gene="nrdG"
                     /locus_tag="VBE22_000423"
     CDS             complement(19106..19723)
                     /gene="nrdG"
                     /locus_tag="VBE22_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEO7201899.1"
                     /translation="MSEEASWNTPKPGEWKSEELSHGHIIDYKAFNFVDGEGVRNSLY
                     VAGCMFHCKGCYNTATWSFRAGIPYTKELEDQIMTDLEQPYVQGLTLLGGEPFLNTGI
                     LLPLLQRIRRELPEKDIWSWTGYTWEEMMLETQDKLEMLSLIDILVDGRFDQSKRNLM
                     LQFRGSSNQRIIDVQKSLKEGEVVIWEGLNDGDNSYEQVKRDDLL"
     gene            complement(19796..20287)
                     /locus_tag="VBE22_000424"
     CDS             complement(19796..20287)
                     /locus_tag="VBE22_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7201900.1"
                     /translation="MKLRRPVLEDKEEILAMYKEFQKESSSVDGGFYEPTMHFEDWLD
                     HNLNMELGVGVPDNFVPYIQFVSFDNDNNAIGFLNLRLRLNDTLLEKGGHIGYSIRPR
                     QRGKGYAKEQLKLGIEQAHLKNINEILVTCHVDNDASKSVILANGGVLEDCLHQTERY
                     WIT"
     gene            complement(20296..21228)
                     /locus_tag="VBE22_000425"
     CDS             complement(20296..21228)
                     /locus_tag="VBE22_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7201901.1"
                     /translation="MLQLGIVGLGGISQKAYLPYMRQVTGVHWHLFTRQKQILEEVNM
                     LFGSSTAYDSLDSLAEHPLDGVFIHVATSAHFDIAKLFLKKGIPVFMDKPLTEDYTST
                     KALYDLAKDHKTFLMAGFNRRFAPRIMEMKKVEDKNHIRTFKNAVNAPADFQYKLFDM
                     FIHPLDTALFLTNNVVKRGYFVTKRDGNKILQVSVTLETDSEIIEASMNLQSGSRREI
                     IEIESPEVTYSLDDLSNLSVIDGFDRRAIGFGSWASTLEKRGFEPMIDAFIQAITTGV
                     NPISPKSSLLSHFICDQINKANAPFGMLNLKIME"
     gene            complement(21241..21384)
                     /locus_tag="VBE22_000426"
     CDS             complement(21241..21384)
                     /locus_tag="VBE22_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201902.1"
                     /translation="MGKYQLDYKGQAQVQKFHEKHSTGENANQKSRLKDLRKQFLEKA
                     KKK"
     gene            complement(21459..23657)
                     /gene="nrdD"
                     /locus_tag="VBE22_000427"
     CDS             complement(21459..23657)
                     /gene="nrdD"
                     /locus_tag="VBE22_000427"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEO7201903.1"
                     /translation="MMVLERERFMTESDIKVIKRDGRLVSFDKYKIYTALLKASNKVI
                     KMSPLVEAKLEMIADHVIAEIYNRFKDNIKIYEIQNIVEHKLLEANEYAIAQEYINYR
                     TQRDFERSQATDINFSIGKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAQLNYQKHLKDAKEWVIEDKQEDYAR
                     AKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTNWFEREIQKAILKIRIQGL
                     GSEHRTAIFPKLIFTLKKGLNLEEDSPNYDIKQLALECATKRMYPDVLSYDKIIDLTG
                     SFKAPMGCRSFLQGWRDANGQDVTSGRMNLGVVTVNLPRVAMESNGDMDKFWEIFNER
                     MSIARDALVYRVERVKEAIPANAPILYQYGAFGERLGKYDNVDRLFNHRRATVSLGYI
                     GLYEVASVFYGGDWEDNHQAKAFTVDIVRKMKQLCADWSDEYDYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPETGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYQCQFEGDFTPTDRGFTCPNCG
                     NSDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSSIKNQGN"
     gene            complement(23756..25297)
                     /locus_tag="VBE22_000428"
     CDS             complement(23756..25297)
                     /locus_tag="VBE22_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201904.1"
                     /translation="MVENLEKPIGVSYKNSPSMSKRTAIRMKKSSRFSILLYSVLSTL
                     LAIANPLLTYFANGLQTQNLYTGLMMTKGQIPYSDVFATGGFLYYVTIALSYLLGSSI
                     WLLIVQFIAYYVSGIYFYKLVYYVAQSEIVSIGMTLIFYIMNIVLGFGGMYPIQWALP
                     FMLISLWFLIKFCVDNIVDEAFIFYGILAAFSLFIDPQTLIFWLCSFVLLTATNIKQK
                     QSLRGFYQFLCVVFGMILIAYTVGYFMFNLQIISSYIDKAIFYPFTYFARTNHSFLLS
                     LAIQIVVLLGSGCLFGLWDFIQNRKKASYQIGLNFIACIFIIYAIMAIFSRDFNLYHF
                     LPALPFGLLLTSNKITILYQKVIDRRSHRRQYFSGKSLIVDLFVKKTYYLPLLLVSLS
                     IGLLVYNTYQNVTLSKERRDISHYLTTKIDRDGKIYVWDKVASIYSQTRLKSASQFVL
                     PHINTAQKNNEKILKDELLQHGAKYFILNKNEKLPNELKSDIKKHYQEVPLSNITHFV
                     LYRFK"
     gene            complement(25547..25864)
                     /locus_tag="VBE22_000429"
     CDS             complement(25547..25864)
                     /locus_tag="VBE22_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEO7201905.1"
                     /translation="MAHNHNHDHNHEHQHEVITLVDENGNETLFEILLTIDGREEFGK
                     NYVLLVPAGAEEDEQGEIEIQAYSFTENADGTEGDLQPIPEDSDAEWDMIEEVFNSFL
                     DEE"
     gene            complement(25890..26309)
                     /gene="ruvX"
                     /locus_tag="VBE22_000430"
     CDS             complement(25890..26309)
                     /gene="ruvX"
                     /locus_tag="VBE22_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEO7201906.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEESGNFGFDR
                     LAELVKEYKVDKFVVGLPKNMNNTSGPRVEASQAYGDKITELFNLPVEYQDERLTTVQ
                     AERMLVEQADISRGKRKKVIDKLAAQLILQNYLDRMF"
     gene            complement(26309..26575)
                     /locus_tag="VBE22_000431"
     CDS             complement(26309..26575)
                     /locus_tag="VBE22_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEO7201907.1"
                     /translation="MGFTDETVRFRLDDSNKVEISETLTAVYRSLEEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDL"
     gene            complement(26777..27175)
                     /gene="spx"
                     /locus_tag="VBE22_000432"
     CDS             complement(26777..27175)
                     /gene="spx"
                     /locus_tag="VBE22_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEO7201908.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHQLPYKEQNLGKESLTRDEILE
                     ILTKTESGIESIVSSKNRYAKALNCNIEELSVNEVIDLIQENPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNVENAEARLRAAL"
     gene            complement(27391..28530)
                     /gene="recA"
                     /locus_tag="VBE22_000433"
     CDS             complement(27391..28530)
                     /gene="recA"
                     /locus_tag="VBE22_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEO7201909.1"
                     /translation="MAKKTKKAEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIVEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYSSVRLDVRGNTQIKGTGEHKDHNVGKETKIKVVKNKVAPPFREAF
                     VEIMYGEGISRTGELIKIASDLDIIQKAGAWYSYNGEKIGQGSENAKKYLADNPAIFD
                     EIDHKVRVHFGMTEDDSPVQSELVEEKNEADDLVLDLDNAIEIEE"
     gene            complement(28604..29863)
                     /locus_tag="VBE22_000434"
     CDS             complement(28604..29863)
                     /locus_tag="VBE22_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEO7201910.1"
                     /translation="MRAEIIAVGTEILTGQIVNTNAQFLSEKMAELGIDIYFQTAVGD
                     NEERLLSLLDIASQRSQLVILCGGLGPTEDDLTKQTLAKFLGKSLTVDLLASRKLDRF
                     FSSRPQFARTPNNERQAQLVEGSIPLQNLTGLAVGGIVTSKGVQYMVLPGPPSELKPM
                     VMEQVVPILSNNGTKLYSRVLRFFGIGESQLVTILEDIIKNQTDPTIAPYAKVGEVTL
                     RLSTKAENQDEADFKLDSLEKEILALKTLDNRKLKDLLYGYGDNNSMARTVLELLKVQ
                     NKTITAAESLTAGLFQSQLAEFSGASQVFNGGFTTYSMEAKSQLLGIPKKKLQEYGVV
                     SHFTAEAMAQQARQLLKADFGIGLTGVAGPDELEGYPAGTVFIGIATPEGVSSIKVSI
                     GGKSRSDVRHISTLHAFDLVRRALLKI"
     gene            complement(29952..30503)
                     /locus_tag="VBE22_000435"
     CDS             complement(29952..30503)
                     /locus_tag="VBE22_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEO7201911.1"
                     /translation="MKRCSWVNLDNPLYVAYHDKEWGRAVHDDHVLFELLCLETYQSG
                     LSWETVLNKRQEFRQVFHHYNIEKVAAMSDADLEIILQNPRVIRHRLKLFSTRQNARS
                     IILIQKEFGSFDRYIWSFVDNKVQVNSVNNYNDVPASTTLSERLSKDLKKRGFKFVGP
                     TCLYSFIQAAGMVNDHENICDFK"
     gene            complement(30526..31116)
                     /gene="ruvA"
                     /locus_tag="VBE22_000436"
     CDS             complement(30526..31116)
                     /gene="ruvA"
                     /locus_tag="VBE22_000436"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEO7201912.1"
                     /translation="MYDYIKGKLSKITAKFIVVETAGLGYMIYVANPYSFSGYVNQEV
                     TIYLHQVIRDDAHLLFGFHTENEKEIFLNLISVSGIGPTTALAIIAVDDNEGLVSAID
                     NSDIKYLTKFPKIGKKTAQQMILDLSGKFVEASGESATSRKVSSEQNSNLEEAMEALL
                     ALGYKATELKKVKAFFEGTNETVEQYIKSSLKMLMK"
     gene            complement(31118..32350)
                     /locus_tag="VBE22_000437"
     CDS             complement(31118..32350)
                     /locus_tag="VBE22_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7201913.1"
                     /translation="MKEFFALPKQLQLRELLRFISITVGSAIFPFMAMYYVQYFGNLV
                     TGILIIITQLSGFVATLYGGHLSDAMGRKKVVIIGSLLATIGWAITIAANVPNHITPH
                     LTFVGILIIEIAHQFYFPAYEAMTIDLTNEQNRRFVYTIGYWLVNIAVMLGSGIAGIF
                     YDHHFFELLIVLLIISAICCFVVYFKFDETKPQEGTFKHDKGVLGTFKNYSQVLVDKA
                     FVVYTLGAIGSSVVWLQVDNYFSVNLKQNFEVVSILGHTITGAKMLSLAVFTNTLLIV
                     LLMTTINKFIENWPLKRQLILGSLICGFGMLFNISLNTFGAILIAMTFFTFGEMIYVP
                     ASQVLRAEMMVEGKIGSYSGFLAIAQPVASVLAGAMVSLSYFTGKIGVQITLTIFMLA
                     GLVLILYATKMKNIEIGK"
     gene            complement(32382..34361)
                     /gene="mutL"
                     /locus_tag="VBE22_000438"
     CDS             complement(32382..34361)
                     /gene="mutL"
                     /locus_tag="VBE22_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEO7201914.1"
                     /translation="MNLSKIIELPDILANQIAAGEVVERPSSVVKELVENAIDAGSSQ
                     ITIEVEESGLKKIQITDNGEGMTSEDAVLSLRRHATSKIKSQSDLFRIRTLGFRGEAL
                     PSIASISLMTIKTATEQGKQGTLLVAKGGNIEKQEVVSSPRGTKILVENLFFNTPARL
                     KYMKSLQSELAHIIDIVNRLSLAHPEVAFTLINDGKEMTKTSGTGDLRQAIAGIYGLN
                     TAKKMIEISNADLDFEISGYVSLPELTRANRNYITLLINGRYIKNFLLNRSILDGYGS
                     KLMVGRFPIAVIDIQIDPYLADVNVHPTKQEVRISKERELMSLISTAISESLKQYDLI
                     PDALENLAKTSTRSVDKPIQTSFSLKQPGLYYDRAKNDFFIGADTVSEPIANFTNLDK
                     SDGSVDNDVKNSVNQGATQSPNIKYASRDQADSENFIHSQDYLSSKQSLNKLVEKLDS
                     EESSTFPELEFFGQMHGTYLFAQGNGGLYIIDQHAAQERVKYEYYREKIGEVDNSLQQ
                     LLVPFLFEFSSSDFLQLQEKMSLLQDVGIFLEPYGNNTFILREHPIWMKEEEVESGIY
                     EMCDMLLLTNEVSVKKYRAELAIMMSCKRSIKANHTLDDYSARHLLDQLAQCKNPYNC
                     PHGRPVLVNFTKADMEKMFKRIQENHTSLRDLGKY"
     gene            34581..34784
                     /locus_tag="VBE22_000439"
     CDS             34581..34784
                     /locus_tag="VBE22_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO7201915.1"
                     /translation="MTQGTVKWFNSEKGFGFISSETGTDVFAHFSEIKVDGFKTLEEG
                     QKVTFDIQDGQRGPQATNINLVK"
     gene            complement(34841..37417)
                     /gene="mutS"
                     /locus_tag="VBE22_000440"
     CDS             complement(34841..37417)
                     /gene="mutS"
                     /locus_tag="VBE22_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEO7201916.1"
                     /translation="MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAA
                     QILEISLTSRNKNAEKPIPMAGVPYHSAQQYIDVLVELGYKVAIAEQMEDPKKAVGVV
                     KREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQATLLTDFE
                     SVRSEILNLKAREIVVGYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEI
                     SAAEKLLQYVHRTQKRELSHLQKVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSL
                     YWLLDETKTAMGTRMLRTWIDRPLVSMNRIKERQDIIQVFLDYFFERNDLTESLKGVY
                     DIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIIVNKIDTMPELE
                     SLINTAIAPEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGT
                     LKIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGEMLEAREQS
                     SNLEYDIFMRVRAQVESYIKRLQELAKTIATVDVLQSLAVVAENYHYVRPKFNDQHQI
                     KIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQLALTVIMAQ
                     MGGFVSADEVDLPVFDAIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFD
                     ELGRGTATYDGMALAQSIIEYIHDRVRAKTMFATHYHELTDLSEQLTRLVNVHVATLE
                     RDGEVTFLHKIESGPADKSYGIHVAKIAGLPIDLLDRATDILSQLEADAVQLIVSPSQ
                     EAVTADLNEELDSEKQQGQLSLFEEPSNAGRVIEELEAIDIMNLTPMQAMNAIFDLKK
                     LL"
     gene            complement(37474..37911)
                     /gene="argR"
                     /locus_tag="VBE22_000441"
     CDS             complement(37474..37911)
                     /gene="argR"
                     /locus_tag="VBE22_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO7201917.1"
                     /translation="MNKIERQKRIKRLIQSGQIGTQEEIKLHLKNEGIDVTQATLSRD
                     LREIGLLKLRSPEGKLYYSLSTATSNRFSPALRSYILKVSRASFMLVLNTNLGEASVL
                     ANFIDEKGLPEILGTMAGADTLLVICQNEDIAKVFEKELSVGL"
     gene            38133..39824
                     /locus_tag="VBE22_000442"
     CDS             38133..39824
                     /locus_tag="VBE22_000442"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEO7201918.1"
                     /translation="MDTKHLIASEIQKVVPDMEQSTILSLLETPKNSSMGDLAFPAFS
                     LAKTLRKAPQIIASDIAEQIKSDQFEKVEAVGPYVNFFLDKAAISSQVLKQVLSDGSA
                     YATQNIGEGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFDKIGYHPVKINHLGD
                     WGKQFGMLIVAYKKWGNEEAVRAHPIDELLKLYVRINAEAETDPSVDEEAREWFRKLE
                     ANDPEATELWQWFRDESLLEFNRLYDQMNVTFDSYNGEAFYNDKMDEVLELLESKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSIYVVGNE
                     QSAHFKQLKAVLKEMDYDWSDDMTHVPFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKDKVAQAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKANFSPSNSDNYSLNDVESWEIIKLIQDFPRTIVRAADNFEPSIIA
                     KFAINLAQCFNKYYAHTRILDEDAEISSRLALCYATATVLKESLRLLGVDAPNEM"
     gene            39912..40220
                     /locus_tag="VBE22_000443"
     CDS             39912..40220
                     /locus_tag="VBE22_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO7201919.1"
                     /translation="MPSEKEILDALSKVYSEEVIQADDYFRQAIFELASQLEKEGMNS
                     LLATKIDSLINQYVLTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(40247..41119)
                     /locus_tag="VBE22_000444"
     CDS             complement(40247..41119)
                     /locus_tag="VBE22_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO7201920.1"
                     /translation="MLKLDLKTKIKETILIAFGVALYTFGFVKFNMANHLAEGGISGV
                     TLIIHALFGVNPALSSLLLNIPLFILGARILGKKSLLLTIYGTVLMSFFMWFWQQIPV
                     TVPLKNDMMLVAVAAGILAGTGSGLVFRYGATTGGADIIGRIVEEKSGIKLGQTLLFI
                     DAIVLTSSLVYINLQQMLYTLVASFVFSQVLTNVENGGYTVRGMIIITKESESAAATI
                     LHEINRGVTFLRGQGAYSGREHDVLYVALNPSEVRDVKEIMADLDPDAFISVINVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(41227..42171)
                     /locus_tag="VBE22_000445"
     CDS             complement(41227..42171)
                     /locus_tag="VBE22_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO7201921.1"
                     /translation="MKTRRTPLEKKVKYIISVWAKKFGLLHTLKSISREKYAEKISAS
                     LLYGILSSVAVNFFFQPGHVYSSGATGLAQVISAVSKHWFSFEIPVALAFYAINIPLL
                     ILSWRKIGHKFTIFTFITVTVSSIFIQLMPQITLTTDPLINAIFGGLIMGAGVGFSFK
                     SRISSGGTDIISLTIRKKTGRDVGSISFIINGIILLFAGLLFGWKYALYSMVTIFVSS
                     RVTDAIFTKQKKMQAMIVTSKPDCVIKRIHRDLHRGVTCINDAEGTYNHEKKAVLITI
                     LTREEFSDFKYLMLKADPKAFVSVAENVHIIGRFVDDD"
     gene            complement(42161..43912)
                     /gene="aspS"
                     /locus_tag="VBE22_000446"
     CDS             complement(42161..43912)
                     /gene="aspS"
                     /locus_tag="VBE22_000446"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEO7201922.1"
                     /translation="MKRSMYAGRVRSEHIGTSITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEEVSASVMATAESLRSEFVIEVSGVVTAREQANDNLPTGEVELKVQELSI
                     LNTSKTTPFEIKDGIEANDDTRMRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDNLEF
                     IDVETPMLTKSTPEGARDYLVPSRVNQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSDQEIQDIVEGMIAKVMKDTKGLEVSLPFPRMAY
                     DDAMNNYGSDKPDTRFDMLLQDLTEIVKEVDFKVFSEASVVKAIVVKDKADKYSRKNI
                     DKLTEIAKQYGAKGLAWLKYADNTISGPVAKFLTAIEGRLTEALQLENNDLILFVADS
                     LEVANETLGALRTRIAKELELIDYSKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     AETAHELEGDLAKVRAVAYDIVLNGYELGGGSLRINQKDTQERMFKALGFSAESAQEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SEQQLEELSLTVESYEN"
     gene            complement(44005..45285)
                     /locus_tag="VBE22_000447"
     CDS             complement(44005..45285)
                     /locus_tag="VBE22_000447"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEO7201923.1"
                     /translation="MKLQKPKGTQDILPGESAKWQYVENVIRNLFKQYHYDEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFHDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PTKMYYIGSMFRYERPQAGRLREFHQVGVECFGSNNPATDVETIAMGHHLFEDLGIKN
                     VKLHLNSLGNPESRQAYRQALIDYLTPIREQLSKDSQRRLNENPLRVLDSKEPEDKLA
                     VENAPSILDYLDESSQAHFDAVCHMLDALNIPYIIDTNMVRGLDYYSHTIFEFITEIE
                     DNELTICAGGRYDGLVSYFGGPETPAFGFGLGLERLLLILDKQGISLPIENTIDLYIA
                     VLGSEANLAALDLAQSIRHQGFKVERDYLGRKIKAQFKSADTFNAKVIMTLGSSEVDS
                     KEVGLKNNQTRQEVKVSFENIKTDFSSVLKQLGL"
     gene            45505..45687
                     /gene="rpmF"
                     /locus_tag="VBE22_000448"
     CDS             45505..45687
                     /gene="rpmF"
                     /locus_tag="VBE22_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEO7201924.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            45703..45852
                     /gene="rpmG"
                     /locus_tag="VBE22_000449"
     CDS             45703..45852
                     /gene="rpmG"
                     /locus_tag="VBE22_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO7201925.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(46000..46521)
                     /locus_tag="VBE22_000450"
     CDS             complement(46000..46521)
                     /locus_tag="VBE22_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEO7201926.1"
                     /translation="MRTYSDKNELKEEVLKSYKKYIAEFNDIPEKLKDLRIDEVDRTP
                     AENLAYQVGWTTLILKWESDEQSGLEVKTPTETFKWNQLGELYQHFTETYASLTIKEL
                     TAQLNDNVDAIGNMIDSMSDEVLFKPHMRNWADSATKNAVWEVYKFIHINTVAPFGTF
                     RTKIRKWKKVALK"
     gene            complement(46619..48103)
                     /locus_tag="VBE22_000451"
     CDS             complement(46619..48103)
                     /locus_tag="VBE22_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO7201927.1"
                     /translation="MRDNGSRVLIISSWRPDPYKAGNVLVKFAMRFTHPITKKSHKKY
                     LSTGASKGWFTTKATPSKKLPSGKERLLVSDIKNTQLITQVTQELNKLVDDYIAELMG
                     IKPKKAKKLLTLEEIAKPFDKDGNFYGKAFKAWHERVKPANNTLKTRVTIYNRYIEPN
                     FDTRMSITKFAFMTDEIQNLINASSMHMARNLHIYLKMIFDWSVENGQITLTQDPIAS
                     NKVKRRVLTKSEEQDKKREDIAEKYLEASEVNHVLRLIESWTNRPDNQLIADVLRMIF
                     LTGMRPSEVLGLNEDMLDFEKKWIKVHWQRASKNKSDDMMEALNLDEKERYRADLKTK
                     ESVRTIPMSPEVEKILRHYIDRNKFQAQFSPTYQDLGYLFTRTYIRAGNRQGSPLYHN
                     ELSQFLRGGSSQSAKYNKKAGKPYKDIDSFLDFGRPIHVIPHMFRHSFISIMASEGID
                     LPTIREFVGHSEDSKEIERVYLHVIKKQKDTMRGAVEKLEKLIE"
     gene            complement(48113..48361)
                     /locus_tag="VBE22_000452"
     CDS             complement(48113..48361)
                     /locus_tag="VBE22_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201928.1"
                     /translation="MATLKTEHDFDLTRNWYRKSVFLDELWHGMTVATLNSYIRQMKD
                     SPYAFGIKGTHGNVFIHSEVFVAWFDYKIANQYVAKMA"
     gene            complement(48427..49455)
                     /locus_tag="VBE22_000453"
     CDS             complement(48427..49455)
                     /locus_tag="VBE22_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO7201929.1"
                     /translation="MDNSVMLDYLAVTIKGLAPDDVIEKILILPKDKFVLNEWGINKY
                     QRHYSFSEIKVYFNKDWQSKMGVFIELRGQGCRQYEEYMENNVNNWVTLMKRISECHS
                     NVTRLDIANDIFDDSLSVPLIYSYCKKQLCISTAKTFDYHEKSLLENGEKVGEMVTIG
                     VRGTQQWCVYNKLLEQKLDQELPNTPLSWTRAELRCWQEKANLLAKQIKEGRPLKEIY
                     FEVINGHYRFVSPRDKDSNRWRRKTVKWWNDYLETQEKTVLSVKRTKPTLKRSEKWTE
                     KQVSRTLGKLYVAKAESHGQEKADSYIQHLLELGISKLTETDETDIQQYKQEQLSSAY
                     WGIRKDDL"
     gene            complement(49458..49889)
                     /locus_tag="VBE22_000454"
     CDS             complement(49458..49889)
                     /locus_tag="VBE22_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201930.1"
                     /translation="MTKFNLTDETIKVGDLKLDKSQFDIPKKVTILSSRVAPVYKNVN
                     GESIATDEVAKITCSVQDTDKIQALKDLGYSSDDLKGIRLEIVDNLDKLAKSVEKDGL
                     NSLVVELVNPEVHLGWRANNNGGGNWGGLKLVATDIKFIGA"
     gene            complement(50165..50620)
                     /locus_tag="VBE22_000455"
     CDS             complement(50165..50620)
                     /locus_tag="VBE22_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201931.1"
                     /translation="MNEYDYQEVVDRVDGLNSVSTLKKWRLKIESLTGTQFKESRVRT
                     GRKSYSKIYLFTDDDLAYFQAVADKKSSLGLERAILSVYGSSKGKDHQKPIRELVDEL
                     EVSFMEIQEDYQRNLTELKQKLEVLLVRIQNLEKETGDKTSKRIGFFKR"
     gene            complement(50620..50835)
                     /locus_tag="VBE22_000456"
     CDS             complement(50620..50835)
                     /locus_tag="VBE22_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201932.1"
                     /translation="MNQTSIELQMVEWVAYSPETQEKVFIKPEDCKDLDDRELYHLIK
                     STGFERLIESRALQIVTDFLNQKTQVS"
     gene            51158..52078
                     /locus_tag="VBE22_000457"
     CDS             51158..52078
                     /locus_tag="VBE22_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7201933.1"
                     /translation="MFEILKPDKKAVGKRLRQVKDELNLSFTDFGNRLGLKKSTINAY
                     VRGDNLAPLEVLEKVSKIYGKPVGWFYYGELEEYIELYLRKLGYGTFLDEYPETPLKI
                     KDELFRIKANPKQLITTNGGVTIGTFENPNWENDFGYPNEGYLDDIFFEMLCETLKNS
                     IRQEAQTYLSQQEGLDDTKIKELSNYFANQIYISYDFVGNLENLSKDKIKQEVKESYE
                     RLLKDSPQAGVEVSYDSNYLLGFLINTLADSQTTRQLLSDMSQATVGMPFSQFHEGHE
                     LVEVIQSVRPRLIELYNNTTFDDWTDWAEK"
     gene            52099..53220
                     /locus_tag="VBE22_000458"
     CDS             52099..53220
                     /locus_tag="VBE22_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEO7201934.1"
                     /translation="MDNKFKNIPKRHLPQKLPFDYTSIYTDPQIIKLTTRANIAIGTY
                     EGFLESIINPMLLISPLLSQEAVLSSKLEGTHATLKDLLNYEAGNKVDIERDELHEII
                     NYRKALFYALENISTINNIDSKGLPLSNRIIKEMHKILLDNVRGSSKNPGNFKRSQNY
                     IGSVSSISYTPVPAEKTPEYMSNLEQYIHYDDLDLLVQSAIIHAQFEMIHPFEDGNGR
                     IGRLLIPLFLYYQELLSYPTFYMSSYFERDRSLYISHLSNISKDNNWKDWFEYYLEGV
                     ILSAEESTKKAQDILSLYNIMKEQVIPKLNSVSGIQLLDFIFSAPIFKAEQVSEHLKI
                     SKRTTYTLLNKLIDEGYLSTDNAQRNRTYYCPQLLSIVQ"
BASE COUNT        16822 a        10884 c         8485 g        17526 t
ORIGIN      
        1 ggttgtttct atctaaaata atctattatt aaaatcgatt aatcaagtcc atgactcctg
       61 cggctaacag tttgtcaaag atgtagataa cgattgtgaa aaatacagtg tattccaaga
      121 tggaaataaa atctttccaa cgttgttttc gatttggcca agtagtgtca cgcaatactt
      181 gaaaaatccc tttgacaaac attatcagtt ctcctttttg cagtttgtta cgctatatca
      241 acgcgtttct ttgtgtattg tatacttctt acaatattta caaaatttat ttacttctag
      301 tcgtgttggc ttgggggtgc tacttactcc gattgagtaa tttcggcttc cgcattctgt
      361 acacgctagg cttgctttct tttgtgccat aacgcctcca taagtgctat tttaacatga
      421 tttgtataat cctgcaagct atctaaagta gtcctttaga ctattccaag catcttttac
      481 cttctcttgg aaatttgaat cttccaccgc tctagaaata tcttgtgcag ccttacttgc
      541 cgaatctttt aaatcattaa taattgagcg tgattcttgg ttcgttgtat tactagcatc
      601 ttgtgcatta taaactgaac caataccgtt ttggaaataa gcattctcaa tatgagtaaa
      661 actagacccc ttagtatacg gtagtatata tgaagcttgc gtgctaaata tatttgaagc
      721 tgtacctgca ctagaatctg tcaaataatg gtgtttatct gtgttcttaa agccaaccca
      781 ttgactaata acgacatcag gcgtatagcc gactacccac tggtctccag ataaattggg
      841 gttaaaatct gtttcggttg ttccagtttt acctgccatt gtataaccat aaacattggc
      901 attaatagct gtaccatttg agaatgttcc taacatcata ctagtcattt tactagccac
      961 tgagcgacta ataactcgct taggcttatc tgtaaattgc tttactaatt ttccattagc
     1021 tgtctcaatt cgagtaatca aatgagcgcg atgcattata ccgtcatttg caaaagttga
     1081 gtaagcttgt gccatttcta aaggattagt ggttacactt ccacctagcg ccactcctag
     1141 ctctttgttt gctgatgaca tatttagacc aaacttcctg ccataagtaa aggctttatt
     1201 gattcctagt ttgtctaaag tataaacagc aggaatatta taagaattag ctagtgcttg
     1261 atacatcggg atactttcag tctcaatccc accatagtta cttggtttat aaccatgaaa
     1321 atcttgaact ttattaggaa gttctttatc tattgaccat cctgaagcaa tagctggtga
     1381 ataaacaact aaaggtttta tggttgaggc tggacttctc tttgattgag tcgcgtagtt
     1441 aaaacttcta aactgtgcat ctttagtact ttgaacacgt ccaactaaac ctcttacacc
     1501 accagttttt ggatctaaag ctacgctagc accttgggca gattgaccat catagtcaga
     1561 tacagggaag aggctagtat catcaaatgt tttttgcatt cctgattgat aattttgatc
     1621 taatgcagtg taaatcttat agccattatt gacaatatct ttttctgaaa taccataagt
     1681 atcaatggct tcattgacaa cagcatcaaa ataggatggg tacctgtaat cattaatttt
     1741 accagcatag gtatctgcta atctattttt cataccaatt gaagcagctt ctttagcttg
     1801 agacttggtt agtttacctg catcaaccat cgctgccaaa acagtatctc ttcggttagt
     1861 tgcattttca acagaataat aaggattata aacttctggt cctttaagca ttccagcaag
     1921 tgtagcagcc tcatctactg tcaaattagc agcactggtt ccaaaatatt tcctactagc
     1981 atcttccaca ccccaaacac cattaccaaa ataagagtta ttaagataca tggtcagaat
     2041 ctcagccttg ctatatttct tagtcaattc tagcgctaaa aagaattcac gcgccttgcg
     2101 tttaatcgtt tggtcctgtg acaagtaagc attttttgct aattgctggg taatagttga
     2161 gcctccgcca aatttcccaa gtgtagctac tgccaaaaaa aatcgcttaa agtttacacc
     2221 attattttca taaaaagttc tatcttcagt tgctataaca gcatttttga gatgatcaga
     2281 aatagcattt aactctacat aagttccctt ttgtcccgaa agttttccag catattcttt
     2341 attcttatca taaatggtgg tggttgtttt tagagcactc tgtaaatcac taactgtagc
     2401 agttttagaa agataaaaaa gatagctacc aaatataaga ataaaagcca ttaaggcgat
     2461 aaaaagcaac ttcccaatac gatagcgccg ccaaaaacga cggataggat ttttaggaga
     2521 agggaggtaa cgatcaactt tttgtagcca ttcaggtcga gatttagcat tttccttttg
     2581 gtgacgaatg cctcgctgat actgggattt actaggtcgg gtaatatcat caatatcata
     2641 gacttcttcg ccatccaaat tgctagtttc ttctctagtt tcttgttcat tttttcttgt
     2701 catttcgtcg ttgtcttcat caacacgaaa taagtctata aacttatcaa ataatttcat
     2761 agacttatta tatcaatttt caataaaatg ctataataaa accatgtcat tttcattaaa
     2821 aattagaaat ccatacggtg aacataccgt taaagaactc cttgaagatt attttttgat
     2881 tccacgtaag attagacatt ttttgcgtgt taaaaaacat gtacttataa acaatgaatt
     2941 tattaattgg caaactgtcg tccaagaaaa cgatactatt accttaatct ttgatgatga
     3001 ggattaccct actaaaaaaa ttcctctggg cagagcagag cttattgatt gtctttatga
     3061 ggatgaacat cttattatcg ttaataaacc tgaaggtatg aaaactcacg gtaaccaacc
     3121 aaatgaaata gcactgttaa atcatgtatc tgcctattct ggacaaacat gctatgttgt
     3181 tcatcgccta gatatggaga ccagtggagc tgttttattt gctaaaaatc catttatact
     3241 tccccttatc aatcaacgct tagaacgaaa agaaatttgg cgtgaatatt gggctttagt
     3301 tgaaggaaaa ttttcaccta agcatcaagt tttgagagac aaaattggac ggaaccgtca
     3361 tgacagacgt aaacgaatca ttgattctaa aaacggtcaa catgctatga caatcattga
     3421 cgttttgaag tatatccaaa atagtagtct cataaaatgc cgactggaaa ccggaagaac
     3481 ccatcaaatt cgcgttcact tatctcatca tggacatccc ttaataggag accccctcta
     3541 caacccttct tctaataatg aaaggttaat gctacacgct caccgattga ctctatctca
     3601 tccattaact tgcgaaacta ttagcgtaga ggccccttca tctactttcg agaagatttt
     3661 aaacaattat aaaaaaggag ttggataata attatccaac tccttttaat cattattgct
     3721 tagttaatgg cttactgagt agtggtttat ggtaatcaaa ttctccatca ctagatgtct
     3781 gccagatcac tgggatgatc ttattccctt caatttttat tccataggcg tatttaactt
     3841 taagaccacc gatacctcct gttacaaaag ttgtagtatc agtacctgat tcatattgat
     3901 aaaggtagag cccatttccc ttatcctcca acttcgtaac tttagcagaa tggttacttt
     3961 ccttagattt actatccttg taaacttttt tctgagtaac tttaccatcc ttatcaaaag
     4021 tcatcgttac tgtaccgtcc tcaacttcac ctttccaact tcctacaagg ttctctggaa
     4081 aaatggaaac gggatcagcc ttaactttat tagtttcctt agctactgtg atatcttttt
     4141 taccatcatt agaaataagg taactatttc ccgatttttt cataataaaa ttaccagtta
     4201 aattctgaat aatgtggcca tatgttttct tatcaggatc tgttgattta tcataattaa
     4261 aatcataatc ggcagcaaaa ttaacttgat aactttcttt cccaacctgt gtcattttat
     4321 taaggacaat attaggaata gtgaaatggc tcgctttacg attatcagtt acaaattttg
     4381 ctttaatact ttctttcaat cctttgtaaa aatcattttc ggcacctttt tcaaaaactg
     4441 ttcctaaagt ctgagggtct tgcccagttg atacataaag tatcaattga ttgaaagcag
     4501 ttatcaattt ctgtccagct tctttttcat ttaataaacc atcaacatct aatttgattg
     4561 tctgattatc agcgatagag attaattttt gtttatgaga ggttatctca ccatcattaa
     4621 aaactttttt gatataagct tttgaaccat cagtcactgg ataattctct acggaatggc
     4681 taccatcttt taatttagca atacgattat caccaaaata aagattacca tccataagat
     4741 tacttgttac tgtaaaagat ttaaaagcga ccgataaatc aatagtttgg tttttaccat
     4801 cataatcttt tttgagttta atctcttttc cgtcactagt cccttcaagg ctagcagtat
     4861 aatgggttct aggtaaacgt tccaccgtta ctgaaaaatg atctgaattg cttgtcgcga
     4921 cctttttttg attcaacaaa acatccaatt taggaacatt ggttttcaag gtcaaagaca
     4981 taggcttatt agcaatacga taatcaggga atatacctaa gcgactacct acacgcttta
     5041 gatacattga atcatttttg tttccttctt ttatttcctg ccctaattta cgcgcctcac
     5101 ttttagacaa gggctcaaaa ttagcgtaat tggcctcatc atttgtgata gattctcccg
     5161 tatctgacca aaccatgtat tctttaatat tatcaaacgc ttgacctgac gtacgagaac
     5221 gtgctgctac aaaacgacta agtacttgtc cctttgaaaa ataaaagaat cctagactca
     5281 gaagtgatac taaaactata ccaaaaataa ctaagcacac tttaacaact tttttcttaa
     5341 aaaaatcaaa cataccttat cctctcattc actttatatt aaagtaaatt gcttaaagaa
     5401 aagttacaac actgataaaa cagctgtatt tgtacttgta accgctattt ttctattata
     5461 acaaaacgaa aaaaggattg aaaccataat ccgatttcaa cctctaaaat ttttgtctat
     5521 gattatacta agtcctgcaa gaagctttct ccaatcatcg cagtatcaac accaaagtta
     5581 tccgcaactg ttgctgaaat atctgcgaaa tgccctactg ggataagtcc gtttcctgtg
     5641 aaggatgggc tataggctaa gagaggaatg tattctcttg tatggtctgt acctgcataa
     5701 gttgggtcat taccatgatc ggcagtgatc aaaagtaagt ctttgtcacg cattgctgaa
     5761 atgatctctg gaagacgctc atcaaactca tgaagacaat cacgataacc atgaggatca
     5821 cgacggtgac catagagagc atcaaagtca accaaattag taaaggagaa gcctttttca
     5881 aactctgata accccattgt ttttatcaaa gtatcaatac catgactatt tgatttgtta
     5941 tgtcccatat catgattaat gcctgaacca ttgaaaatat cattgatttt accaacagca
     6001 taagtatcga tgccagcttg atctagttta tttaatacgg tatcttcaaa tggtgatact
     6061 gcatagtcgt gacggttggc tgtacgcgta aagttacctg gttcacctac atatggacga
     6121 gcgataatac gaccaaggag agctggacgc tccattgtga tactacgagc atattcacaa
     6181 atccggtaga gttcttctag agggatgata tcctcatgag ctgcaatttg taggactggg
     6241 tcagctgagg tatagataat gagctcacca gtttccattt ggcgtggtcc aaagtcatca
     6301 ataacagctg tccctgaata aggtttattc gcttcacgaa taactttacg tcctgaaaaa
     6361 tcctcaatct ttgtgataat atcttctgga aaaccattcc agaaagtatc aaatggctct
     6421 gtaatattta agcccatgat ctcccaatgc cctgtcattg tatctttacc aagtgatact
     6481 tcttgtaact tcgtcgcgta gccacttgga ttttcttctg cagggacggt cttaagcgct
     6541 tggggacgag gaatattacc aagtccaatt ttagccatgt ttggcaccgc aagtccaact
     6601 gtttttgaaa tgtggccaag cgtgtctgaa gcaccatcag gaactcctgc attgacaaaa
     6661 tcattggcat ctggtgccgc tccaataccc actgaatcta agacaactaa gtgaatgcga
     6721 tcaaattgtg acataacttc tcctttatca attgaaaata ggaagtcaga ccagcatcct
     6781 tcctatttcc tttatattat ttaataagtg ccttcttcaa ctttttggtt cttaacaatc
     6841 ttgatgatac ggctagtacc caagcgtgaa gctcctaaag caataaattt ttcagcatct
     6901 tccaatgatg agattccacc tgctgcctta attttaacac cttcgccgac atattttgcc
     6961 atcacttcaa catcttcaaa tgtagcacct gctgtcgaaa aaccagtaga ggttttaata
     7021 aagtctgcac ctgaacgtgt gacaacccca caaagttcga taagttcttc tttagttaat
     7081 tgacatgtct caacgataac ttttaagata tggtcttgac atgcagcctt gatttgacga
     7141 atttcttctt caacagtatc aaaatcccca tttttaacat ctgtcaaatt aatgaccatg
     7201 tcaatttcat cagcaccatt tttaatagca tcttgacatt caaaaacctt cgctgcagtt
     7261 gtactatagc catttgggaa cccaataaca gtacaaatag ctaatttacc tgaaacgtac
     7321 tctgctgctt ttttgacgta agaggctgga atacatgctg aagctgtttc ataagccatt
     7381 gcatcatcta aaattgtttg gatttctggc catgttgctg ttgttgctag caaagtatgg
     7441 tctaccgttt ttaaaatatc ttttacttcc acgttactat ccttctttct tttgtcaact
     7501 tttagaatag agttacaata ccgacgataa aggcgctaag catacttaca gcaataccac
     7561 caatcattgc acggaaaaca agtcgagcta gggtgccacg tttctctgga caaagaacag
     7621 caatacctga aacacaaata cctaaacttg ataaattagc aaaaccacaa agtgaaatag
     7681 ttgctaccaa tgctgtacga taatctaaag atttgattag gtcacccaat tgttggaacg
     7741 aaacaaactc atttaaaatc aacttacttc caagaaggtt tccttctaga agaatgtttt
     7801 tgtggtcaaa tcccataaga aaaccaaatg gagcaaaaac atatgagaag atttgttcta
     7861 agcggattcc caatccactt aacatcatat taatcaaaga aactaaacca acaaaggcaa
     7921 tcaaactagc accaattgag aaagccattt gtgcacctgt gcttgcaccc tcagcgattg
     7981 catcaatcac attggcgtta ttacctttat tatccatctt aatgtcatca attttttgaa
     8041 caggttctgt ttgaggcaat aagattttag caatgagaat actgccaata ggaaccattg
     8101 ttgaagcaat caagagatat tccattggaa tgcctaatgc aatgtagcca ccaagaatag
     8161 aaactgacat acttcccata cctgatacca acacaaccat tatctcacta tcagtcatac
     8221 gacctaggta tttgctaacc aaaattggac tgtctgtttg accaagaaac atattagcta
     8281 cggcaacaaa actctcaacc tctgaggatt tcataatttt accaacgccc ttacctatcc
     8341 attttactac aaatccaagg attcctacat aataaagtag actaactagg gcagataaga
     8401 aaacaatatt gccaagcgtt tgaatggcga aaataaaacc agtttttgct ccactatctg
     8461 ctaatgaacc aaacacaaaa tttaatccag cttgaccaca gttgattact ttagtaactc
     8521 cagttgaaac aacactaaca acttgttgac ctagtgggat acgtactaag attagcgcaa
     8581 taatgaattg aacgataaga gcttttccaa ttaaacttag agaaacactc ttacgattga
     8641 aagaaattgc atacacaatt cctaatacca ataaaatacc aataatacta taaataaatt
     8701 gcatgatgac ttccaaaacc tcgcttattg tgatttatca ttttcaataa gtgtgcggag
     8761 tgcttcaaca gcaacttgaa tagctgcttc tgtgtcatga gccattgggt tatccatacc
     8821 caaagcgttg cgttcttgat taccaacaac aaggaaatct gatccacaac gaactcctag
     8881 atgacttgct gctacaaaaa gagctgcaga ttccatttca gacgcttttg ttcccagacg
     8941 tttccatgct tcccatttat ttaataattc ataactaact ggcatacgtt ctggctcatg
     9001 ttgtccataa aatgcatctt tacattgaac tactcctgcg tgacttgtgt aaccaagctt
     9061 tttagctgca ttaactagag cattcgttac ctccaagtca gctacagcag gaaattcaat
     9121 cggagcatat tctttacttg taccttccat acggatagct ccagttgcaa taacgatgtc
     9181 tccgccttta acatcaaggt caataccacc acatgttcca acacggataa aggtatcagc
     9241 gccacagagt ttcaattctt ccatcgcaat agacgctgat ggaccaccaa taccagttga
     9301 agtaacactg actttttcac cgtttaatgt acctgtataa gtaacatatt cacggctgtc
     9361 tgctacaaga actgcattat caaaatgttc tgcaattttt gcacaacgtt ttggatcacc
     9421 tggcatgata acataacgac ctacatcacc tggacgaatc tgtaggtgat attgcaatcc
     9481 gacttcacct gaataattct gcatgataaa gacctcctta atgtcttgtc tattttttta
     9541 cacttgtata ataacataca agacaacctt tgtaaacgct tttatcgaaa gaaaacgttt
     9601 tattttttaa cttgattttt ctaatttttt caattaaact aacttgtata cggaggttac
     9661 aatgcctaaa aatgaactaa ataataaact aaataaacta aagcatgtac aagtctacaa
     9721 taagattttt aacatgattc aagacggtac ttattctcct ggaatgcaat taccttctga
     9781 acccgaatta gcagggcaac ttaatgttag tcgagctact ttacgaaagt cattagcact
     9841 attacaagaa gaccatttgg ttaaaaatat cagaggaaaa ggtaatttca ttagagaaaa
     9901 ttctagcaac ttgtctgaaa acggttacga aaatagacaa caccctatta aaacttgtct
     9961 aacttccaaa attacagaag tagaattaga atttcgtgta gaggttcctg cagaagctat
    10021 cacagcttca cttaaacaag aaacccctgt agttgttatt gctgatcgtt ggtaccacac
    10081 cgatgatgga ccattagctt acacactttc ttttatccct atcgaactta tttcagatgc
    10141 tgagatttct ttacatgata ctaaacagtt gttaaacttc attgaagaag gtatctatca
    10201 agaaggcata tcaagccact cacaaagcca tttgggatat gcgacatctg gcaatttttc
    10261 agcaactaaa tataccctat ccgatcatgg acaattcata ttaatacaag aaacaatttt
    10321 taagcaagag aaaatactaa tgtgtaataa gcattacgtt cccattgagc actttgaatt
    10381 gtccattacc tctcattaag gaggcaagcc tatgcaaaat agactattaa aagttgcttt
    10441 tttcataact tttaccgaat taattatggg cctaatagtt ggcgcttatt ttaagttaag
    10501 ttattttcta gcccctccaa aggaaccgtc aatgggtgct gggtacggaa tgctcttttc
    10561 tctgttattt atacttatta gttacttaat tctattagct ataattcaat tcttagctta
    10621 tcaatctcaa tctaagataa tagcaatcat tgttttgcta cttttttcct cccactactg
    10681 gcaaatatta gatggcctat ccttatgatc ttcttaccgc aagtggtagt gactatcata
    10741 tctctattat cacaacataa aactcccctt tgttaaaggg gagttttatg ttgtgataag
    10801 ttagtgatta gaagccaccc atcatagatg gatccattgc aggagctgta ggagcttctg
    10861 gttcaggttt atttgctact actgcttctg tagtcaagat aagacttgct acagatgccg
    10921 cattttgaag tgcagaacgt gttactttga cagggtcaat gatacctgtg gtaaccatat
    10981 ctacccattc tccattggcc gcattaaatc ctgtaccaat ttcagactgt tttaaacgtt
    11041 caataataac tgaaccttca tatccagcat tgtaagcaat ttgacgaaca ggctcttcga
    11101 gagcacgaag aacaatatta cgtccagtct cctcatcacc attaagtttc agtgccgcta
    11161 ctttttcaat aacgttcaca agagccgtac ctccacctga aacaatacct tcttcaactg
    11221 cagcacgcgt tgcatttaac gcatcttcga tgcgaagttt catctctttt aattctgttt
    11281 cagtcgctgc accaacttta attactgcta caccaccggc taacttagca agtcgttctt
    11341 gtaatttttc acgatcaaaa tcagaagttg tagcctccat ctgtgactta atgatagcta
    11401 cgcgattagc aattgctgat gagtcaccgg caccttcgac aataacagta gaatctttat
    11461 ctactgttac tttagcagac tgtcctaaaa cttgcatagt agcatctttt aagtctaaac
    11521 caaggtcttc agtaacgaca gttcctcctg ttaggatagc aatatcttcc agcatggctt
    11581 tacgacgatc accaaatcca ggcgctttaa cagctacgac attgaaagtt ccacgaattt
    11641 tgttaagaac aagcgttggg agagcttctc catcaacatc atcagcgatg attagcaacg
    11701 gacgatttgt tttaagaacc tcttctaata atggtaaaat ttcttggata tttgaaattt
    11761 tcttatctgt aataaggata tacggattct caagttcaga gaccattttc tcgttatcag
    11821 ttaccatata ctgtgacaag tacccacggt caaactgcat tccttccaca acttcaagct
    11881 ctgtttccat acctcgcgat tcttcaatag tgataacacc atcattaccc acgcgctcca
    11941 tagcttcaga aatgtattcc ccaacttttt cagaacgtga agacacagcc gcaacttgag
    12001 caatagcttc tttgcctgaa actggttgtg caatctcttt tagctcttca actgctgctg
    12061 aaacagctgt ttcaatacca cgacgaatgc caatcggatt tgcccctgca gttacatttt
    12121 taagaccttc ccgtacaata gcttgggtca aaacagtagc agttgtagtg ccatcccctg
    12181 caatatcatt agtttttgaa gccacttctg acacaagttt agctcccata ttttcaaagt
    12241 gatcttctag ctcaatttct ttagcaattg tcacaccatc atttgtaatt aaaggagaac
    12301 caaatgcttt ttcaagaaca acattacggc ctttaggacc taatgttact ttgactgtat
    12361 cagctaaaat atcaacacca cgcaccattg ctgagcgggc atctgctgaa aatttaatat
    12421 cttttgccat aatttaatac ccaggcctaa aatttaacaa agtctacttt ctttacaaag
    12481 caacttttct ctaataggcc tatatccttt cttgcttaat aaaacctatt taacgacagc
    12541 aacaatgtca ctttcgcgaa caactgtcac tttttcgtta ccgtctttaa cttccagacc
    12601 agcaccattt tcgactaaaa cagtatcacc ctctgcaacg ctcggtgcaa ctaattcccc
    12661 tgttaaggta cgaataccgt cacccacagc aagaactttg gctgtctttg tcgcatcatg
    12721 gcttgctcct gcaagaacaa aaccaccgac agatttctct tcagtttcaa caaacgaaat
    12781 gatcacacga tcacctaatg gttttaacat aataaacgaa cctccatacg agatatattt
    12841 tgatttttta gcactcctat acagagagtg ctaactacaa aaactattct atcacttggt
    12901 cagaattagt caagagaaaa ggcacgtttc aaacaagaaa aatttcccta gttatcctgt
    12961 ccattcacta gagaaattta tattttaacc taagacaagg gcatcatcta tcaactgttg
    13021 acctgagttt ttatggaaaa gttccattaa ttctgcaact gttaagtttc tctttgcttc
    13081 tcctttaaca tcaacaacaa ttttcccgtg gtagagcatc acgagacgat taccataagc
    13141 aatggcatgc tccatattgt gcgtaatcat taaggctgtc aatcgttgtt cttctatcac
    13201 tttctgtgtt aattccataa ccatatcgct agttttggga tctagagccg ctgtatgttc
    13261 atccagtaat aataatttgg gacgaacaag agttgccata gctaacgtga gggcttgacg
    13321 ctgaccgcca gataaaaatg cagcatcagt cttcattcga ttttctaagc ctagacctaa
    13381 ttgtgataga gactttttaa atagttgacg gtcatcgtca gtcactgact gtctaaaaat
    13441 gtgacgctta ttgcctcttt tatgtgcaat tgccatattt tcttcaatcg tcaaatttgt
    13501 tgcagttccc attctaggat cctggaagac tcgactaata tccttagatc gtttttctac
    13561 agagtcttta gtaattgatt ggttgtctaa tgtgattgcc ccctgatcaa tagggatcag
    13621 accagcgata cagttaagga gggttgattt tcccgcaccg ttacctccaa ttactgaaat
    13681 aaaatcaccg tcttcaatcg ttaaatctaa accacgtaaa acatggttct cattgacagt
    13741 tcctttttca aaagttttgt gcagattgac taattcaagt aatgacatgt tcttatcctt
    13801 cctttgtaag tgatttagag gtttttactc ctagtttagc tcgtaattct gggacataca
    13861 aaatgagagc caacaaaatt gctgaaacca actttatcat ctgtgcatca atatcggttg
    13921 ttaatatgaa aacaataatc atacggtata aaacagaccc taatacaatt gaccataacc
    13981 ttttgccaag aggtaagtat tttatcatta cttctgccaa gataattgaa gctaatccaa
    14041 taacgattgt acccactccc atattcaaat ctgcatagcc attattttgc gctaaaagtg
    14101 caccagataa agcaatcagg ccattcccaa tcatatatcc tagcattttc atccgatcta
    14161 ctttaatact attggcttgt cccatagcct cattatcacc tgtcgctctt aaagctaatc
    14221 ctagttgggt atttaaaaag agatacaaaa ttaagataac caagcctaca caaacaattc
    14281 caattaataa aactgctgaa agtttattta aacctaatct tgttaccatt gtaaccaaag
    14341 tatttttgag agcaaaagag acgtttgatc ttcctaaaac aagtaaatta atagagtaaa
    14401 gtcctgtcaa cgtaataata cctgtgagga gggctggtat tttcatttta gtatgaagaa
    14461 aacccgatac tagtcctgcc aacataccac ctaacatgcc cgctatggtt gccacaattg
    14521 gattaatatc attaacgata cataaagcgc aaactgcagc ccccataggg aaagcaccct
    14581 ctgcagagag atcggctatg tctaagatac gaaaagtgat aaacacgcca attgccataa
    14641 ttgaccaaag taatccttgc gaaattccgg ataagataat atctaacata caataccttt
    14701 ctgattattt attattttta atacttgaaa catcaattcc aagctctttt gccatatctt
    14761 cattaactac tacttttaga ttttgaggat attctgaagg aactttagcg acgtctttac
    14821 ctttgaggat tttaacagct tgtcttgccg tttgacgacc taaagcctca taatcagccc
    14881 cataagaaaa gagcaaacca cttggtacca tatctgctga acctcccaca acaggcactt
    14941 ttaattcttt cgatagattc cctaacagcg ttactgagct ggctataata ttatctgttg
    15001 gtacaaaaat aacctctgtt ttagacatta aactcttagc agtatcttgt acatcattag
    15061 tggaagaaat acctttaaca cttgttttaa tccccgcctc ttggaagatt ttttttgctt
    15121 gttttacctg aacttctgag ttacgctcgc tagtggtata cataatacca acacgtttaa
    15181 ccttaggcat tacttttctc aaaagggata cttgcttttt aattggagac atgtcacttg
    15241 taccagtcgc taaaccttca ggttttttca ttgattttac taattcggct gataccggat
    15301 ctgtcactgc tgtaaataaa ataggtgttt ctgttgacac cgttgtcaac gattgagccg
    15361 ccggagtcgc aattcccaat actaatttat tatctttaat gagtttttca gaaattgatt
    15421 ggatatttga ctgatcccct tgagcattct tatactcaat tttgatattt ttgccatctt
    15481 tatagccttc tttagctaat tcttttataa atcctttcct agtggcagtt agagagggat
    15541 gttctacata ttgtaaaata ccaatatgtt ttacatcaga ttgaccattt tgagaagatt
    15601 gtgaacaagc tgtcatcaga acagttgcta aaacgctgct aattaccatc tttgttattt
    15661 ttttcattac tatctccttg taataccatc aaattttcca taaaacaaaa aaccaactag
    15721 accctacatc tagttggtta attatttctc tcaattcctc tgtgcacggg agggcgaaaa
    15781 aatattgcct taaccaacta gattattgaa atgatctaat cggctctgct ttcatcataa
    15841 aatgctttag cttcatagat acaaactttg cgtgacgttt gtatccttga ggctacaaac
    15901 gtctatctaa agaaactaaa gtaataataa gtatttgttt gtttaataat agacgatttg
    15961 attaacatat tattctctcc ttgtttatgg ttgcctataa gtttacgtca tttaaaaata
    16021 attgtcaata gttaaatttg atttttttga aaattcttgt tagatagata atttaaataa
    16081 tagtcattcg ttgatataac acgcttttaa ggaaatttag atttgctatc cttaattttc
    16141 catatactct actaaagcat aataaatacc atcgttatca ttatcctgag ttacatttcc
    16201 aattctctct tgtaaatctt taggagcatt ccccatgaca aaacctttac ccacaatttc
    16261 aagcagattt aaatcattat agttatcacc aaacgcaata gagtctgcaa gagaaatatc
    16321 taaatagtct gctaaagttt tagcagattt tcctttttca atacctggag ccattatttc
    16381 aataaagtaa ggagcagatt gtgcgattga taaatgtgga tattgagctt tcaaagtgtt
    16441 ctccaattct cccatcagag atggttctcc catcaagaga agtttatgaa tcctatcttg
    16501 cttctctaac tccatcaaac tcatctcttt agattgtaaa ccaacaatct cctcctcttt
    16561 ttggacccac tcattttctt tatcacagga ataccattca tgataacgat agacattcca
    16621 tgcaattttc gaataatgct tgcttacata agaacaaact tgtaaggcaa cttgaccatc
    16681 cattgtgtgg gaagcaattg ttttttgttg catatcttgt attaaagccc cattatagga
    16741 aataatataa gaatctattc caatttgttc cataatcgga gtaattgcct caggcatcct
    16801 agctgatatg ggaacgaaag tagccccttg agctactatt tgtttaattc cctgccgtgt
    16861 cttaggtgtt actttaaaat tactgttaat taaagtacca tcaatatcac taaatattag
    16921 ccttaccata ttatcctcca aaatataatt gtacacgctt actttagtct aacacttaaa
    16981 tttttgtttc gcaaataaac ttcgtctggt ctaatgagac tactaatata agctatattt
    17041 caaaacaaat tagtttattt ctctcaatga acggtgtaat atgttactat atagtaataa
    17101 taggaggatt aactatgtcg tatccataca aagccaatca ttccattgaa tcaatcacct
    17161 taaaagtaaa tgacctcgaa aacctcgtaa acttctatag cgatatcatt gggttaactg
    17221 ttattgacaa atcaagcact agagccttac taggggtaaa tcaaaaaatc cctcttatta
    17281 tccttgaaaa aactgaactt gaaaagcata gtacctatgg cctttatcac actgctattt
    17341 tagtaccaga tgaataccat ctcagtctag ctctaaatca tttattatct cagcatatcc
    17401 ctctagaggg tggagctgac catggctata gcaatgctat ctatctaagt gatcctgaag
    17461 gtaatggaat tgaaatttat aatgataagg atatctctat gtgggatatc agagagagtg
    17521 gacaaattat tggtattact gaaagactag atattgacaa tttacttgac agccttgtca
    17581 acgttcctaa taattataag ctttctgaaa aaactagtat tggtcatatt catttgagtg
    17641 ttaaagatgc taagattagc tcaaaactct atcaaaatgt ttttggcctt gatgagaaat
    17701 ttgcaatccc gacagctagt tggattgcat ccggcaacta ccatcatcat ctagccttta
    17761 ataattgggc tggtccaaat ctttctaaaa accaagaaga tagacccgga atttccttat
    17821 tgacaattgc ttacaatgat gacaaccttt ttagagatag cttaaaaaaa gcgcaacttt
    17881 accaactgac tttccttgaa aaacaagatc attattatat tattgaagat tttgatggca
    17941 tccgaattaa agttgttcta aaaccagaga attaaccatc acttgacttt tgcctcttca
    18001 ttttacataa tgtcaatatg ataaaaattt tgatcccaac tgcaaaagaa atgaaagtgt
    18061 gtcaaaacat tgcatggcct aaactgagtg ctcaaacgaa aattataatt gattattttt
    18121 ctactttaac tgtttcagac ttagaggata tctacagaat taatacaagt gcagctcgtt
    18181 gtgaggctca aagatggcaa gattttaaag ctaaacaact tactctgaat cctgctatta
    18241 agttattcaa cagtcttatg taccgcaata tcaaaaggca caacttatcc acaagtgaag
    18301 cacaatttat ggaaaattct gtttttatta ctagtgctct ttatggcatc attcctgcta
    18361 tgacactaat atcccctcat cgtttagatt ttaacactaa aatcaaaatc aacaataact
    18421 ctcttaaagt tttttggcgt gaaaattacg atacttttat gcagtcagat gacattatgg
    18481 tttctttgct ttctaatgaa tttgaaactg tcttttctcc taaagagcgt caaaaactaa
    18541 tacatcttaa ctttattgaa gatcgagatg gccaattaaa aacccactct accatatcaa
    18601 aaaaagcaag aggaaagtgt ttaactgcta tgatggaaaa taattgccaa actctagaac
    18661 acttaaagca attacgcttt gatggcttct gttatgataa tgaactctca gactctaagc
    18721 aattaacttt tgttaaaaaa cagacttaag taactattta tccacaattt attcacacaa
    18781 aaagcaagag ataatcgtat ctcttgcttt ttgacaaact tgtaaggtct aaaaacctct
    18841 ctatatcaag gtttcaaaca atattaacaa aaaataattg ttattaacaa cttgtggata
    18901 actatgggga tagacagagg ataatctgtt tatataaagt tgattactta acactaatgt
    18961 cattgtatat acctttagat ttcaaaaata cttgttaaat taacaataat aaacgtcaac
    19021 atcttgtcac agactgtcaa atacttataa acaagttgtg gataaacata acttttaatt
    19081 tttatttaaa agttaatttt tgacactaga gtaaatcatc tcgtttgact tgttcataac
    19141 tattgtcacc atcgtttaat ccttcccaaa tcacgacttc accttctttt aaagattttt
    19201 gtacatcaat gattctttga tttgaagatc ctctaaattg tagcattaaa tttcgcttac
    19261 tttgatcaaa cctaccatcc actaaaatat caatcaaaga taacatctcc aatttatctt
    19321 gagtctccag catcatttct tcccaagtat accccgtcca tgaccaaata tctttttctg
    19381 gcaattcacg tcggatacgc tgaagaagtg gcaacaaaat acctgtattt aagaacggct
    19441 ctccacctaa taacgttaaa ccttggacgt aaggttgttc taaatctgtc ataatttgat
    19501 cttcaagctc ttttgtataa gggattccgg ctcgaaagga ccatgtagca gtattataac
    19561 accctttaca gtggaacata catccagcta cgtagagtga gtttctaaca ccttcaccat
    19621 ctacaaaatt aaaagcttta taatcaatga tatggccatg acttaattct tcgcttttcc
    19681 actccccagg ctttggagta ttccaacttg cttcttctga catttctaac ctctctattg
    19741 atatgaaaag aaccagaact aactggtctt tttattatta tcttaaagta ctattttagg
    19801 ttatccaata acgctctgtc tgatgaagac aatcttctaa aacaccaccg ttagctaata
    19861 ttactgactt gctggcatca ttgtctacat ggcatgtcac aagtatttca ttaatatttt
    19921 ttagatgtgc ttgctcaatt cctagcttca actgttcttt agcataacct ttgccacgct
    19981 ggcgaggtcg tatggaataa ccaatatgtc cacctttttc taaaagagta tcatttagtc
    20041 ttaaacgtaa atttaagaaa cctatagcat tattgtcatt gtcaaaagac acaaactgaa
    20101 tatatggaac aaaattatca ggaacgccta cccctaactc catatttagg ttatgatcta
    20161 gccagtcttc aaaatgcata gttggttcat aaaaaccacc gtccactgaa ctactttctt
    20221 tttgaaactc tttatacatg gctaaaattt cttctttatc ttctaacact gggcgccgta
    20281 atttcatatt gtttcctatt ccattatctt caagttaagc attccaaaag gagcattcgc
    20341 tttattaatc tggtcacaaa taaaatgact caataaacta gactttggtg atataggatt
    20401 gacacctgta gtgatagctt gaataaaagc atcaatcatt ggttcaaaac cacgtttctc
    20461 tagagtgctt gcccaagaac caaaaccgat agcacgacgg tcaaagccat caataacaga
    20521 aagatttgac aaatcatcta atgaataggt tacttctggc gactctattt caattatttc
    20581 tcgacgagac cccgactgca ggttcatgct tgcttctatt atctcactgt ctgtttctag
    20641 cgttactgat acttgtaata tcttattccc atcacgttta gtcacaaagt atcctctttt
    20701 aacaacatta tttgtcaaaa atagcgccgt atctaaagga tggataaaca tatcaaaaag
    20761 cttatattga aaatccgctg gtgcattgac agcattttta aaggtgcgaa tatggttttt
    20821 atcttctact tttttcattt ccataatacg tggtgcaaag cgacgattaa aacctgccat
    20881 taaaaatgtt ttatgatcct tcgccaaatc atacaatgct ttagttgaag tataatcttc
    20941 tgtcaaaggc ttatccataa aaactggaat accctttttt aaaaaaagct ttgctatatc
    21001 aaagtgagca ctagtcgcga catgaatgaa gactccatcc aaaggatgct cggctagact
    21061 atctaaactg tcataagctg tagaagaacc aaaaagcata ttaacttctt ctaaaatctg
    21121 tttttggcgt gtaaaaagat gccagtgcac acctgtcact tgacgcatat aaggtagata
    21181 agctttctgt gaaatacctc ctaaaccgac aattccaagt tgcaacatag catcctcctt
    21241 ctattttttc tttgcctttt ccaaaaattg tttccttaaa tcttttagac gtgatttttg
    21301 attagcattc tctccagtag agtgcttctc atggaacttc tgtacttgtg cttgcccttt
    21361 gtaatctaat tggtatttcc ccatatctta ataactcctt ctattttatc ttcttatttt
    21421 aacatataat agaccagagg ttttaataga ggttttaatt aattaccttg atttttaata
    21481 cttgatccgt tcatatgttt tacacgagca gagatttcct tatgacgacc attaaccatc
    21541 ggacgagcct gtggattacc taaataacca catgtacgtt tgacaacatc tactgtctta
    21601 gggtcgctgt taccacaatt tggacaagta aaacctcgat ctgtcggtgt aaaatcccct
    21661 tcaaattgac attggtaaca cttatcaatc ggtgtattag tacctagata acctacccga
    21721 tcataagcat agtcccaaac tgcttctaaa gcttttggat tctgttgcaa aacagggtat
    21781 tcacagtagt gaataaatcc tcctgatgca cccgtttcag gatagatttt ctcaaaatct
    21841 agtttttcaa aaggtgttgg attcttacgc acatcataat ggaatgaatt agtgtagtat
    21901 tccttatcag taatatcagt tactatacca aatttttcag tatctaaacg acaaaaccgg
    21961 tcagtcaagc tctccgatgg tgtcgaataa actgaaaaat gataatcata ctcatcagac
    22021 caatctgcac ataactgctt catcttacga acgatgtcta cagtaaatgc tttagcttga
    22081 tggttatctt cccagtcacc accataaaaa acactcgcca cctcatataa accaatataa
    22141 ccaagagaca ccgttgcacg gcggtgatta aagagtcgat caacattgtc atattttcct
    22201 aaacgttctc caaatgctcc atactggtaa agaatgggcg catttgctgg gatagcctct
    22261 ttgacacgtt ccacacgata aaccaaggca tctctagcaa tactcatacg ttcattaaat
    22321 atctcccaga atttatccat atcaccgtta gactccatgg caacacgcgg tagattaact
    22381 gttacaactc caagattcat acgaccagat gttacatcct gaccatttgc atctcgccac
    22441 ccttgtagga aagaacgaca gcccataggg gctttaaaag agcccgttaa gtcaataatt
    22501 ttatcataag aaaggacatc tggatacatc cgttttgtcg cacactctaa tgctaattgt
    22561 ttaatatcat aattggggct atcttcttct aaattaagac cttttttaag cgtaaaaatt
    22621 aattttggaa aaatagcagt acgatgctcg cttcctaaac cttgaatccg aattttcaaa
    22681 atcgcttttt gaatttctcg ttcaaaccaa ttagttccta atccaaaacc aagcgaagta
    22741 aatggcgttt gcccatttga agtaaataaa gtattaattt catactccaa agattgcatg
    22801 gcatcatata tatctttttg tgttttagcg cgcgcatagt cttcttgttt atcttcaata
    22861 acccattctt ttgcatcttt taaatgtttt tgataattta actgtgcata aggagctaaa
    22921 aactcatcaa tgcgatctgc tgtacagcca ccatattgac ttgaagcaac gttcgcaata
    22981 atctgtgaaa tctgagcagt cgctgtctga atagattttg gactctcaac ttctgcatta
    23041 ccaatcttaa aaccatttgc aagcattccc ttaaaatcga ttaaacagca atttgtcatg
    23101 ggagtgtatg ggctgtaatc taaatcatga taatggatat cacccttttg atgtgcattt
    23161 gctacatgag aaggtaacat tttcagacca attgatttcc ctacaatacc tgctgttaaa
    23221 tcacgttggg tattaaacac atcagagtct ttattagcat tctcattaac aacagtttga
    23281 tccttattaa ttaattttcc aatagaaaaa ttgatatctg tagcctgtga acgctcaaaa
    23341 tcgcgttggg tacgataatt gatatattct tgtgcaatag cgtactcatt agcttccaat
    23401 aatttatgtt caacgatatt ttgtatttca tatatcttga tgttatcttt aaaacgattg
    23461 tagatctcag cgatgacatg atctgcaatc atctcaagtt tagcctcaac taaaggagac
    23521 attttgataa ctttattact agcttttaat agagccgtgt agattttata cttatcaaag
    23581 ctgaccaaac gcccgtcacg tttaatcact ttgatatcag attctgtcat aaatcgttct
    23641 ctttccaata ccatcatgtg aaatatcctc cagtgacaat tatagcatgc aaagaaaaaa
    23701 aatcaatata ttgtgtcaag ttttttgctc agacacaata tattgatttt ttaatctatt
    23761 tgaaacgata tagaacaaaa tgagtgatat ttgatagagg aacttcttgg tagtgcttct
    23821 taatatcaga tttcagttca tttggtaact tctcattttt atttaagata aagtatttag
    23881 ccccatgctg aagcagctcg tccttcaaaa ttttctcatt atttttttgc gctgtattaa
    23941 tatgtggcaa aacaaattga gaagcagatt ttaaccgagt ctggctataa atgctagcaa
    24001 ctttgtccca aacataaatc ttaccatctc tatcaatttt tgtagtcaaa taatgtgaaa
    24061 tgtcacgcct ttctttacta agagtaacat tttgataagt attataaact agtaacccta
    24121 tcgataagga aactagtaag aggggcaaat aatatgtttt ctttacaaaa aggtctacga
    24181 taagagattt accagagaaa tactgacgtc tatgtgaacg tctgtcaatc actttttggt
    24241 aaagaatagt aattttatta ctcgttagca aaagaccaaa tggtaaagca ggtaaaaagt
    24301 ggtaaaggtt aaaatctctt gaaaaaatgg ccattatagc gtaaataata aaaatacagg
    24361 caataaagtt caagccgatt tgataagatg cttttttacg attttgaata aaatcccata
    24421 gtccaaagag acaacctgac cctagaagga caacaatttg aattgctaaa ctaagaagga
    24481 atgagtggtt tgtcctagcg aaatacgtaa aggggtaaaa aatagctttg tcaatataag
    24541 aagagataat ctgtagatta aacataaagt aaccaacagt ataagcaatt aaaatcatac
    24601 caaaaacaac gcataaaaac tgataaaatc ctcgtagtga ctgcttttgc ttaatattgg
    24661 ttgctgttaa gagaacaaaa gagcaaagcc aaaaaatcag tgtttgggga tctataaata
    24721 gtgagaatgc tgctaaaatg ccataaaata taaatgcttc atcaacgata ttatcgacac
    24781 aaaatttaat taaaaaccat agcgaaatga gcataaaagg taatgcccac tgtattgggt
    24841 acataccacc gaatcctaag acaatattca ttatatagaa aatcaacgtc atgcctatcg
    24901 agacaatttc actttgtgcc acataataaa ctaatttata aaaataaatt ccagatacat
    24961 agtaagcaat aaactgtaca ataagtaacc agatactaga ccctaaaagg taacttagag
    25021 caatcgttac atagtataaa aatcctcctg tagcaaaaac atcactataa gggatctgtc
    25081 ctttggtcat catcaaacct gtatagagat tctgagtctg caaaccattt gcaaaataag
    25141 tcaataaagg atttgcaatt gctaagagag ttgacaaaac actatatagt aaaattgaaa
    25201 atcgactaga tttcttcatt ctgatagccg ttcgtttact cattgatggg ctatttttat
    25261 agctcactcc aattggtttt tctaggtttt ctaccactat ttttctcctc atatttacat
    25321 actaccagta tatcagaaaa ccttatttag tcaatttagc caacacttga ccgtaacgaa
    25381 taatatccct aaagtagtgg tattttctag acatatattg tttgagaaat tcttgttctt
    25441 ctagtgttag ctgagtatct gacattaaca tcacctcctt ttactacatc atcactatta
    25501 ttattcgaaa aaaaaacgac tgaacaattg ctcaatcgct tcgatattat tcttcatcta
    25561 gaaagctgtt gaaaacttcc tcaatcatgt cccattctgc atctgaatct tctggtatag
    25621 gctgaaggtc tccttcagtt ccatcggcat tttctgtaaa tgagtaagct tgaatctcaa
    25681 tttcaccttg ttcgtcttct tccgcacctg caggaacgag gagaacatag tttttgccaa
    25741 actcttcacg cccatcaata gttaatagaa tttcaaaaag tgtttcgttc ccattctcat
    25801 ctacaagagt gattacttca tgttgatgtt catggttatg atcgtggtta tgattatgtg
    25861 ccattgtttc tcctttttat gccgaatgct taaaacatgc ggtctaaata attttgtaag
    25921 attagttgag ctgctaattt atcgataact ttcttacgct taccacgact aatatcagct
    25981 tgctcaacta gcatacgctc tgcttgaaca gtcgttaaac gttcatcttg atattcaact
    26041 ggaagattga ataattcagt gattttatcg ccatatgcct gactagcctc tacacgagga
    26101 ccacttgtat tattcatatt tttaggtaga ccaactacaa atttatcaac tttgtactct
    26161 tttactaact ctgctaagcg gtcaaaacca aaatttccag attcttcatc gatcttaatg
    26221 atttcaagac cttgtgcagt gaaaccgagt ggatcactaa ttgcaacgcc gacagttttc
    26281 gagccgacgt caagtcccat tattctcatt acaaatctat tccatttcct tgtaaataat
    26341 aacgaacgag ctcttctaca atctcatcac gttcatattt acgaatttga ttgcgagcat
    26401 cattgtaacg aggtacataa gcagggtccc cactcaatac gtaaccaaca atttgattga
    26461 tgggattgta acctttttct tctaatgaac gataaacagc tgttaatgtt tcactaattt
    26521 ctactttgtt actatcatct aatctaaaac gaacagtttc atctgtaaat cccataccta
    26581 caccttcttt cctttaattc tttcacttat tataacttaa ataaagtatt tgtacaagaa
    26641 attgataaac actatattag aaaatcaaaa agggagcaca agaaaccaag aagtgattct
    26701 ttgatttctt tactccctac cttataggtg atttttacag gttatcgtgt gactctcact
    26761 tatgcttatc taaagctcaa agtgctgcac gaagacgagc ttctgcattt tcaacgttac
    26821 gaatagagcg aggtagaaaa gcacgaatat catcttcctt ataacctact tgaagacgtt
    26881 tgtcatcaat tagaattgga ctctttaaaa tacgtggatt ttcttgaata agatcaataa
    26941 cttcatttac actcaactcc tcaatattac aattgagcgc cttagcataa cgatttttag
    27001 aagaaacgat actttcaata ccactttcag tttttgttaa tatttctaaa atttcatcac
    27061 gtgttagtga ttctttacct aggttttgtt ctttatatgg aagttgatga gcatttagcc
    27121 aagtttttgc ttttttacaa cttgtgcaac ttgaaatagt ataaatctta atcatgtgcc
    27181 ttctcacttt ctatcccatg atagttatag tttaacatag tcacaaaaat caaacatggg
    27241 acttaagacc tattttttat caatttacta ataaaaataa atagatttta gggattttta
    27301 agcttatttt tagtataaaa ataagttcca accactgtca gaacttattt tagttacatt
    27361 tattcaagtt caagatgtat agttgctatt ttattcctca atttcaatag cattgtccaa
    27421 atctaaaaca agatcatcag cttcattttt ttcttcaacg agctcagact gaactggcga
    27481 atcatcttca gtcattccaa agtgcacgcg cactttatgg tcaatttcat caaaaattgc
    27541 tggattatct gctaaatatt ttttggcatt ttcagaacct tgtccaattt tctcaccatt
    27601 atatgagtac catgcacctg ctttttggat aatatccaaa tcactagcaa ttttaattaa
    27661 ctcacccgtg cgagaaatac cttcaccata cataatttcg acaaaagctt cacggaatgg
    27721 tggagcaact ttatttttaa caactttgat ttttgtctct ttaccaacat tatgatcttt
    27781 atgctcacca gttcccttga tttgagtatt accacgaaca tctagacgaa ccgatgaata
    27841 aaatttcaaa gcacgaccac caggtgttgt ttcagggtta ccaaacataa cgcctacttt
    27901 ttcccttaac tggttgataa aaattgcgat tgtctttgtc ttgttaatgg atgctgacaa
    27961 tttacgcatt gcttgactca tcatacgagc ttgcaaacca acgtggctgt caccaatatc
    28021 accatcaatc tcagcacgag gaacaagggc tgcaactgaa tcgacaacaa ctaaatcaac
    28081 tgcgcctgaa tcaatcaatt tacctgcaat ttcaagacct tgctcacctg agtcaggttg
    28141 agacaaaagt aactcatcaa tattaactcc aagagccgca gcataggctg ggtcaagagc
    28201 gtgctcagca tcaataaagg cagcaatccc accttccttt tgggcttgag ctacggcatg
    28261 aagggcaacc gttgttttac cagatgactc tggtccataa atttcgacga tacgtccttt
    28321 aggatatcca cctgctccta aagcgatatc taaagcaaga gaacctgaac tcattacttg
    28381 aaccttttgt tcagctcgct cacctaagcg catcactgcg cccttaccaa aatctttttc
    28441 aatatttttc aaagcatcat ctaaggcttt tcgacgttca tcaccgaatt tcttagtaat
    28501 ttcttctgct tttttcgttt ttttagccaa tgtcttctcc ttatatttct atgaatctag
    28561 gttgattcaa atttagaaat cgttctacta attataacaa atattatatc tttaataaag
    28621 ccctacgcac caagtcaaaa gcatgtaaag tactgatatg acggacatct gagcgactct
    28681 tccctccaat cgatacttta atagaagaaa cgccttcagg ggttgcaatc ccaataaaaa
    28741 ctgtacctgc agggtatcct tccaactcat caggaccagc aactcccgtt aaaccgatac
    28801 caaaatctgc ttttaaaagt tggcgagctt gctgagccat tgcctcagct gtaaaatgac
    28861 tgacaactcc atattcttgc aattttttct tagggatgcc gagaagttgt gattttgctt
    28921 ccatactata ggtcgtaaaa ccaccattaa aaacttgcga agcaccagaa aattctgcta
    28981 actgagattg aaataaccct gctgttaagc tctctgcggc agtaatcgtt ttattttgta
    29041 ctttcaataa ttctaaaaca gtacgtgcca tactattatt atcaccataa ccatataata
    29101 aatcttttaa tttacggtta tctaatgtct taagagcaag tatttctttt tctaagctat
    29161 ctaatttaaa gtcggcctca tcctgatttt cagctttcgt tgataacctt aaagttacct
    29221 caccaacctt tgcatatggt gctatagttg ggtctgtctg attcttaatt atgtcttcta
    29281 agattgtgac taactgactc tcaccgatac caaagaatct caatacacgt gaatataatt
    29341 ttgtgccatt atttgacaaa ataggtacta cctgctccat aaccattggc tttaattcac
    29401 taggcggacc tggtaaaacc atatattgga ctcccttaga tgtaacgata ccaccaacag
    29461 ctagaccagt aaggttttgt agaggtattg agccttcaac caattgagct tgtcgttcat
    29521 tatttggcgt tctagcaaac tgaggacgac ttgaaaaaaa acgatccaat tttcgactag
    29581 ctaataaatc tactgttaat gatttaccta aaaatttagc caaagtttgc ttcgttaagt
    29641 catcctctgt agggccaagt ccaccacata ggataaccaa ttgactgcgc tgactagcta
    29701 tatctaaaag cgataataaa cgttcctcat tgtctcctac agctgtctgg aaataaatat
    29761 caattcccaa ttctgccatt ttctccgaca aaaattgtgc gtttgtatta acgatctgtc
    29821 ctgttaaaat ttctgttcct acagcgataa tttcagccct cacaacttca ccctcttatc
    29881 tattcgaaat aatgactaca ttttagcaaa aaaagtagca aaagtgctac ttaaaacata
    29941 tcacattgat attacttgaa atcacatata ttttcatggt cattaaccat acccgcggcc
    30001 tgaataaaag aatagaggca agttggacca acaaatttaa aaccgcgttt ttttaaatcc
    30061 ttagataagc gttcggataa ggtagtactt gcaggcacat cgttgtagtt gttaactgag
    30121 ttaacctgta ctttattatc tacaaaagac caaatgtagc gatcaaaact tccgaattct
    30181 ttctgaatta atataattga cctagcattt tgtcgtgttg agaaaagttt tagacgatga
    30241 cgaataaccc taggattttg taagattatc tctaaatccg catctgacat tgcagctacc
    30301 ttttcaatat tatagtgatg aaaaacttgt ctgaactcct gacgtttatt taatactgtt
    30361 tcccaagata aacccgattg ataagtttct aaacatagta actcaaagag tacatgatcg
    30421 tcatgaacag ctctccccca ctctttatca tggtaagcta catataatgg attatctaaa
    30481 ttcacccaag aacatcgttt catgtgaaac ctcattactt ctaaattatt tcatcaacat
    30541 cttcaaacta gatttgatgt attgctccac agtctcgttc gtaccttcaa aaaaggcttt
    30601 tacttttttc aattcagtcg ccttataccc caacgctaac aaagcttcca tagcttcttc
    30661 aagattgcta ttctgctcag agctaacctt acgactagtc gcactctcac cagaagcttc
    30721 tacaaacttc cctgacaaat caaggatcat ctgttgtgct gttttttttc ctatctttgg
    30781 aaatttagtc aaatacttaa tatcactatt atcaatggca cttacgagac cttcattgtc
    30841 atcgactgca ataattgcta aagcggtcgt tggaccaatt cctgatactg aaattaaatt
    30901 aagaaaaatt tctttttcat tttccgtatg aaaaccaaat aaaagatgtg catcatcgcg
    30961 aatgacttga tgtaagtaga tggtaacttc ttgattaacg taacctgaaa aagagtaggg
    31021 atttgccaca tatatcatgt atcccaatcc agctgtttcc acaacaataa atttagccgt
    31081 tattttactc aattttcctt taatataatc gtacattcta ttttcctatc tcaatatttt
    31141 tcattttggt agcatataag attaaaacca gacctgctaa cataaaaatg gtcaatgtta
    31201 tttgaacacc tatttttcct gtaaaatagc tgagcgaaac catggcacct gctaatacac
    31261 ttgcaacagg ttgtgcgata gctaaaaatc cagaatatga tccaattttt ccctccacca
    31321 tcatctccgc ccttagaacc tgactagctg gaacataaat catttcgcca aaggtgaaaa
    31381 acgtcatggc aatcaaaata gctccaaaag tgtttagaga aatattaaaa agcattccaa
    31441 aaccacaaat caagctccct aatattagtt gacgtttcaa aggccaattc tctatgaatt
    31501 tatttatagt tgtcattagt aagacaatta atagagtgtt tgtaaagact gctaaactaa
    31561 gcattttagc acctgtgatt gtatgtccta aaatggacac tacttcaaaa ttttgcttaa
    31621 gattgaccga gaaataatta tcaacctgga gccagacaac actggaacca attgctccta
    31681 aagtataaac aacaaacgct ttatccacta aaacctgact ataattttta aaagttccta
    31741 aaactccttt atcatgcttg aaggttccct cctgtggttt tgtctcatca aacttgaagt
    31801 agaccacgaa acaacaaatc gctgaaataa taagaagaac tattaataat tcaaaaaaat
    31861 ggtgatcata gaaaatccca gcaattccag aacctagcat tacagcgatg ttaaccaacc
    31921 aataacctat agtgtaaaca aagcgacgat tctgttcgtt cgtcaaatca atggtcatag
    31981 cttcataggc agggaaataa aattggtgag ctatttcaat gataaggata cctacaaagg
    32041 tcaagtgtgg cgtaatatga tttggaacat ttgcagctat agttatagcc caaccaatgg
    32101 ttgccagcag tgaacctata atcacaactt ttttcctccc catagcatct gacaaatggc
    32161 ctccatataa ggtcgctaca aaccctgata gttgagttat gatgatcaag attcctgtaa
    32221 ctagatttcc aaaatattga acataataca ttgccataaa aggaaaaata gctgaaccaa
    32281 ccgtaatgga aataaaacgc aataattctc ttaactgtag ctgttttggc aaagcaaaaa
    32341 actctttcac tttttctccc ttcttcccta acaagtaatt atcaatattt tcctaaatcc
    32401 ctgaggcttg tatgattttc ttggattcgt ttaaacattt tctccatatc agcttttgta
    32461 aaattaacta aaacaggtct tccatgaggg caattataag gatttttaca ttgcgctaac
    32521 tgatctaata aatgacgtgc tgagtaatcg tctagcgtat ggtttgcttt aatagatcgc
    32581 ttgcaagaca tcataatagc caactctgcg cgatattttt tcactgatac ttcattagtg
    32641 agcaacaaca tatcacacat ttcataaatg ccagactcta cctcttcttc tttcatccaa
    32701 attggatgtt cccttaaaat gaaagtatta ttaccatacg gttccaagaa aataccaaca
    32761 tcttgtaata atgacatttt ttcttgtaat tgaagaaaat ctgaacttga aaattcaaat
    32821 agaaatggta ctaacaattg ttgaagacta ttatcaacct cacctatctt ttcacggtaa
    32881 tactcgtatt ttacacgctc ttgagctgca tgttgatcaa taatatacaa cccaccattc
    32941 ccttgagcaa ataagtaggt cccatgcatt tgaccaaaaa attcgagctc tggaaaagtc
    33001 gaactttctt ccgaatctaa tttttcaaca agtttgttaa gtgactgttt actactaagg
    33061 taatcctgag aatgaatgaa gttctcacta tcagcttgat ctctactagc atactttata
    33121 ttaggagact gcgttgctcc ttgattaaca gagtttttta catcattgtc aactgaaccg
    33181 tcacttttgt caaggtttgt aaagttagca ataggttcag atacagtatc tgcacctata
    33241 aaaaagtcat ttttagccct atcgtaatac aaaccaggct gttttagtga gaagcttgtt
    33301 tgtataggct tatctacact tcgggtacta gttttagcta aattctctaa agcatccgga
    33361 atcaagtcat attgtttaag actctcagaa attgctgtgc ttattaaact cattaattct
    33421 cgttccttag aaattctcac ctcttgctta gtcggatgaa cgttgacatc tgctagataa
    33481 ggatctatct gaatatcaat gacagcaatt ggaaatcttc caaccatcaa ctttgaacca
    33541 taaccatcta gaattgagcg gttcaaaaga aaatttttaa tgtaacgacc gttaattaat
    33601 agggtaatat agtttcggtt agctcgtgtc aattcaggca aacttacata acccgaaatt
    33661 tcaaaatcta aatctgcatt tgaaatctca atcatttttt ttgcagtatt taaaccataa
    33721 atacctgcaa tcgcctgtct caaatcgcca gtcccactag ttttagtcat ttctttacca
    33781 tcatttatca gtgtaaaagc aacttccgga tgcgctagac tgagtctatt aacaatatca
    33841 ataatatgag ctagttcaga ctgtaaactc ttcatgtatt tcaatctggc aggcgtgtta
    33901 aagaataaat tttcaactaa aatttttgtt ccacgaggac ttgaaacaac ttcctgtttt
    33961 tcaatgtttc cccctttagc aacaagtaac gtcccttgtt tcccttgctc tgtagctgtt
    34021 tttatcgtca ttaaactaat cgaagcgatt gatggtagag cttcaccacg aaaacctaag
    34081 gtccgaatac gaaaaaggtc actctggctt ttgattttgg atgtagcgtg acgtcgtaaa
    34141 ctgagaacgg catcctcact agtcatccct tcaccgttat ccgttatttg aatcttttta
    34201 agaccagatt cttcaacctc tatggtaatt tggctacttc ctgcatctat ggcattttca
    34261 actaattctt taacgacgct actaggcctt tcaacaacct caccagcagc aatctggtta
    34321 gctaatatat ccggtaattc aataatttta gacaagttca tccctccttt ttttatgatt
    34381 ttttgtaact ttttttgtga tatcttagcc attataccat attcaaaata ttaatttgac
    34441 aataacacaa tttttagttg acatcactcc tattacctgt tagacttagg gagcgtaata
    34501 agttttgaag ttgtattttt gtttaacatt tttacctttt ttatttttta cagtaacatt
    34561 tttagaaagg taataaacaa atgacacaag gtacagtgaa atggtttaac tcagaaaaag
    34621 gttttggctt tatttctagc gaaactggaa cagacgtctt cgctcatttt tcagagatta
    34681 aagttgatgg cttcaaaact cttgaagaag gacaaaaagt aacatttgat attcaagatg
    34741 gtcaacgtgg accacaagca acaaatatca atcttgttaa ataattaaaa aagagcctaa
    34801 atgaattatt tagtctcttt tttgttgcct aaaggctatt ttataataat ttctttaagt
    34861 caaatatagc attcatagct tgcattggag ttagattcat tatatctatc gcttctaact
    34921 cctcaataac cctacctgca tttgaaggtt cttcaaaaag cgataattgt ccttgttgct
    34981 tctcagaatc tagttcctca tttaagtcag cagtaacagc ttcttgggag ggcgatacga
    35041 tcaactgtac tgcatcagct tccaactgtg ataaaatatc agttgcccta tccaataagt
    35101 caattggtaa accagctatt tttgcgacgt gtatcccata agacttatcc gcaggtccag
    35161 attcaatttt atgtaagaag gtaacttctc catctctctc taaagtagcc acgtgtacat
    35221 tgacaagcct tgtcaactgt tcagataaat ctgtcaactc atggtaatga gtcgcaaaca
    35281 ttgtttttgc cctaacacgg tcatgaatat attctataat cgattgagct aatgccatac
    35341 catcataagt ggctgtccct cgacctaatt catcaaaaag aatcaaagat ttatcactgg
    35401 ctcgttttac agcttgattc gcttccatca tttctaccat aaaggttgat tgcccagaaa
    35461 ttaagtcgtc agcagcacca atcctagtaa atattgcatc aaatacaggc aaatcaactt
    35521 cgtctgccga tacaaaacct cccatttgtg ccataataac tgtcaaagct aactggcgca
    35581 tataggtcga cttaccactc atatttggtc ctgtaatcaa ctggatatct gtctgactat
    35641 caaaatagat gctattggga atatattctt gcactcccat cactttttca acagttgcat
    35701 gacgcccatt cttaatctta atctgatgtt gatcattaaa tttgggacga acatagtgat
    35761 aattttctgc aactactgcc aaactctgta gaacatcaac ggttgcaatc gtctttgcta
    35821 actcttgaag acgtttaata taagattcta cttgggcacg aacacgcata aaaatatcat
    35881 attctaaatt tgaagattgc tcgcgagctt cgagcatttc accttcaatt ttggctagtt
    35941 ctgctgttcc atagcgttca gaatttttta atgtcgcttt acggaaaaaa tgctccggta
    36001 ctagtgataa attggaattg gtaacatgga agtaataacc gtctttttta ttataatcaa
    36061 ttttaagagt accgatacca cttgctgctc tttccttagc ttcaatatca gcaatccaac
    36121 ctgtaccttc acgcatcact gttcgataat tatccaattg cttatcaaat ccagacttga
    36181 taatgtttcc ctcagtgata gtagcctgtg cttctggggc tatcgccgta ttaattaaac
    36241 tttctaattc aggcatagtg tcaattttgt tgacaatgat gtcaagctca ggttgattga
    36301 aggactgtaa aatcatttta atccgaggaa tttgtgataa ggtctgtccg agttgcaata
    36361 gatctttagg gttggctttt ccgaaagata ctcgacttgc taggcgttca atatcatata
    36421 caccctttaa actttctgtg agatcgtttc tctcaaaaaa ataatcaaga aacacttgaa
    36481 taatatcttg acgttccttg attcgattca tacttaccaa aggcctgtca atccaagttc
    36541 tcagcattcg agttcccatc gctgttttag tctcatctaa caaccagtaa agacttccat
    36601 gcttcttgct tgttctagca ttctccagta aatctagact atttttcgtt gcatatgaca
    36661 tttgtaaata gtcctttatt tcataatgaa ctactttctg taaatgacta agttcacgct
    36721 tttgtgttct atgcacatat tgtaaaagtt tttccgcagc agatatttcc aaatcagtta
    36781 actgctcatc aatcaaatga atatcattaa gtcgttcgtc ttcgtatgat aaaagcaagt
    36841 tcatctgctt cgtcagtagg tgattttttt cgtccgtcaa ttgatatcct actacaatct
    36901 cacgagcttt taaatttagt atttcactac ggacggactc aaaatctgtt aaaagggttg
    36961 cctgaaactc tccagttgag acatccatat atgctagacc aaatgtttgt tgatcttgcg
    37021 aatcaatcgc tactaagaaa ttattagcac tatccggctt cgttgactcc acaaccgttc
    37081 ctggggtaac aacttgcact acctcacgct tgaccactcc cacagctttt ttaggatctt
    37141 ccatctgctc agcaatggct actttgtaac ctaattcaac taaaacgtca atatactgtt
    37201 gagctgagtg atagggaact cctgccattg ggattggctt ttctgcgttc ttatttcgac
    37261 tagtcaagct aatttccagg atttgtgctg cttttaccgc atcatcataa aataattcat
    37321 aaaaatcacc cattctaaaa agcaaaaaag catctggata attctctttt atatccagat
    37381 actgttgcat tcccggtgat atcgttggct tggccatttc tcactcctaa ctaacggggc
    37441 tggctgccat gctcagctcc tcaatcatta tatttataaa cctactgata gttctttttc
    37501 aaaaactttg gcaatatctt cattttgaca aatcaccaac aaggtgtctg ctcctgccat
    37561 tgttcctaaa atctctggca gccctttctc atcaataaag tttgctaaaa cgctagcctc
    37621 ccctaaatta gtatttaaaa ctaacataaa acttgctcgc gatactttca aaatatatga
    37681 gcgtaaggct gggctaaatc gatttgaagt agctgttgat aaactataat atagcttacc
    37741 ttctgggcta cgtaatttta aaagaccaat ctcacgcaag tctcttgata gagtcgcttg
    37801 cgtgacatcg attccttcat tttttaaatg taatttaatt tcttcttgtg tgccaatctg
    37861 ccctgattgg atcaaacgtt taatacgttt ttggcgttct attttattca tgttattcaa
    37921 tcctttttag ttagaattat gcttaccaaa ttatatcaaa aaaaattgtc tttttcactt
    37981 agtagtgata tcgtaatcat tctgtaaaaa agctataaag taggctagtt ctttacagaa
    38041 tgcattatga atttaatcaa caagttctcg tctatttttc ataattatgt taaaataggg
    38101 tgactatatt actaattaaa ataggagata ccatggatac aaaacatcta attgcgagtg
    38161 aaattcaaaa agttgttcca gatatggaac aatcgaccat tctttcttta ttagaaaccc
    38221 caaaaaattc gagcatggga gatttagctt tcccagcatt ctctttggct aaaactctac
    38281 gcaaagcacc tcaaataatt gctagtgaca ttgctgaaca aattaaaagc gaccaatttg
    38341 aaaaggtgga agctgttgga ccttacgtta actttttcct tgataaagcc gcaatctcaa
    38401 gtcaagtttt aaaacaagtc ttatctgatg gttctgccta tgctactcaa aatattggtg
    38461 aaggacgaaa tgttgccatt gacatgtcta gtccaaatat tgctaagcca ttctcaattg
    38521 gacaccttcg ttcaacagtt attggtgata gtttagctaa tatttttgat aaaattggct
    38581 atcatcctgt taaaattaat caccttggcg actggggtaa acaatttgga atgttaatcg
    38641 ttgcctataa aaaatggggc aatgaagagg ctgtccgtgc tcatcccatc gatgaacttt
    38701 taaaacttta tgtccgtatt aatgctgaag ccgagacaga ccctagcgtt gatgaagagg
    38761 cacgtgagtg gtttcgtaaa cttgaagcta acgatcctga agcaactgaa ttatggcaat
    38821 ggttccgtga cgagtcatta ttagaattca accgtcttta cgatcaaatg aatgttacat
    38881 ttgatagcta taacggcgag gcattctaca acgacaaaat ggatgaagtg cttgaactgc
    38941 tagaatctaa aaacctccta gtcgaatcta aaggtgcaca agttgttaac ctagaaaaat
    39001 acggtattga acatcctgcc ctcattaaaa aatccgatgg agcaactctc tacattacac
    39061 gtgacttagc tgctgcactt taccgtaaac gtacctacga ttttgcaaaa tcaatctacg
    39121 ttgtaggaaa cgaacagtct gcacacttta agcaactaaa agctgtgctt aaagaaatgg
    39181 actacgattg gtccgatgat atgactcatg ttccttttgg ccttgtaaca aaaggaggag
    39241 caaaattatc aactcgtaaa ggaaatgtaa ttctacttga acctactgtt gcagaagcta
    39301 ttaatcgtgc ggcatctcag attgaagcta aaaaccctaa cctagcagat aaagataaag
    39361 ttgctcaagc agttggtgta ggtgctatta aattttacga tttgaaaact gatcgtacaa
    39421 atggttatga ctttgactta gaagcaatgg tatcatttga aggtgaaact gggccatatg
    39481 tacaatatgc acatgctcgt attcaatcaa tcttacgtaa agctaacttt agcccatcta
    39541 atagcgataa ttacagtctc aacgatgttg aaagctggga aatcatcaaa ctcatccaag
    39601 attttcctcg tactattgta cgtgcggcag ataattttga gccctctatt attgcaaaat
    39661 tcgctattaa tttagctcag tgcttcaata aatattacgc ccacacacgt attttagatg
    39721 aagacgctga aatcagtagt cgtctagcac tttgctatgc aactgctaca gtattaaaag
    39781 aatctcttcg cctcctagga gttgatgctc cgaatgaaat gtaagaaatg acactactaa
    39841 gactgtcaaa tttatgacag tcttaatttt ttatattata ataatagcac aacaagttac
    39901 aggagaatgc catgccatca gaaaaagaaa tattagatgc tcttagtaaa gtttatagcg
    39961 aagaagtaat acaagcagat gattattttc gtcaagctat tttcgagctc gctagtcaac
    40021 ttgaaaaaga ggggatgaac tcactcctag ctacaaaaat tgactcactt atcaatcaat
    40081 acgtcttaac acatcaattt gatgctccta aaagcatatt tgatcttagt cgcttggtaa
    40141 aaacaaaagc tagccattac aagggaactg ctatctcagc tattatgctt ggtagtttct
    40201 taagtggagg accaaaataa aaatacaggc cagacagcct gtatttttat ttatcgtaat
    40261 ttcttcgtcg aattttaaaa tctgagctaa taacttcatc tacatttata acagaaataa
    40321 aagcatctgg gtctaaatca gccatgattt ctttcacatc acgcacttca cttgggttaa
    40381 gagctacgta caagacatca tgctctctac ctgagtaggc accttggccc cgtaaaaaag
    40441 ttacacccct attaatttca tgcaaaatgg ttgctgctgc agattctgat tctttggtaa
    40501 taatgatcat tccacgaaca gtgtagcccc cattttcaac atttgttaac acttgactaa
    40561 atacaaagct agctactaac gtatatagca tctgttgtaa attaatataa acaagtgaag
    40621 acgttagaac tatagcatca ataaacaata aggtttgccc taattttatt ccagattttt
    40681 cctctacaat acgaccaata atatctgcac cacctgtagt tgcaccatat ctaaatacta
    40741 aaccactacc agttcctgct aaaattccag cagctactgc aactaacatc atatcgtttt
    40801 tcaaagggac tgttacaggg atttgttgcc aaaaccacat gaaaaaagac attaacactg
    40861 taccatatat agttaacaat aaggattttt tccctaaaat cctagcccct aatataaata
    40921 acgggatatt aaggagaagt gatgaaagtg ctggattaac tccgaaaagt gcatggataa
    40981 tcaaagtgac accggaaata cctccttcag ccaaatgatt agccatatta aatttgacaa
    41041 agccgaaagt atagagagcc acaccaaagg caattaaaat agtttctttt atctttgttt
    41101 ttaaatccag tttcaacata ggcttaatcc ttccttattc aaaaaagcta aagttgttta
    41161 aacaacttag cttaataaca tcataatgtt tccttagtgg tggctgacca aaagtcatat
    41221 atacagttaa tcatcatcaa cgaatcgtcc aataatatga acattttctg caacggaaac
    41281 aaaggctttt ggatccgctt ttagcattaa atatttaaaa tcactaaatt cttcacgtgt
    41341 taatatagtt attaaaactg cttttttctc atgattataa gtaccttcag catcattgat
    41401 acaggttaca ccacgatgta aatcacgatg gatacgttta atgacgcaat caggcttact
    41461 ggtaacaatc atcgcttgca tctttttttg ctttgtaaaa atggcatctg taactctact
    41521 tgatacaaaa atggtcacca tcgagtacaa agcgtacttc caaccaaata aaagacctgc
    41581 aaataataaa ataataccat taataataaa agaaatacta cctacatctc tgcctgtttt
    41641 ctttctaatt gtcaaactga taatatctgt tcccccacta gaaattctag acttaaagct
    41701 aaaaccaaca ccagctccca taattaaacc accaaagata gcattgatta agggatcagt
    41761 tgtaagggtg atttgaggca ttagttgaat gaagatagag cttacagtta ctgtgataaa
    41821 cgtaaaaata gtaaatttat gtccaatctt acgccaagat aatatcagca aagggatatt
    41881 aatggcataa aaagctaaag caactggtat ttcaaaagaa aaccagtgtt tactgacagc
    41941 tgaaataacc tgagctaaac cagttgctcc acttgaataa acatgtccag gttgaaaaaa
    42001 gaaattgaca gcaacactgg atagaatacc atacagcaat gaagccgaaa ttttctctgc
    42061 atatttttct cgtgagatac tttttaaagt gtgtaagaga ccaaattttt tagcccatac
    42121 actgataata tattttacct ttttttctaa aggggttcgt ctagttttca taactttcta
    42181 cagtaagtga taattcttct aattgttgtt ctgaaacgag actaggagct tgagtcattg
    42241 gatcactagc cttattgttt ttagggaatg caataacttc tcgaatattg tccttgcctg
    42301 ctaacaacat aacaaagcgg tcaaggccta ttgccaatcc accatgtggt gggaagccgt
    42361 aatccatagc ttcaagtaag aaaccaaact gttcctgagc tgattcagcg ctaaatccta
    42421 aagccttgaa catacgctct tgtgtatctt tttggttaat acgtaggcta ccaccgccta
    42481 actcatatcc attcaaaaca atatcatagg caactgcacg tactttagcc aaatcccctt
    42541 ccaactcatg cgctgtctcc gctgttggca aggtgaatgg atgatgagcc gacatatatc
    42601 gtccctcttc ttctgaccac tcaaacattg gccaatcaac aacccatagg aaattaaatt
    42661 tagaataatc aatgagttca agttcttttg caatgcgtgt acgaagcgct cctaaagtct
    42721 cgttagctac ttcaagacta tctgctacaa atagaattaa gtcattattc tctagctgta
    42781 aagcttctgt caacctacct tcaatagctg tcaagaactt agcaaccgga cctgaaatag
    42841 tattatctgc atattttagc catgctaaac ctttggcacc gtattgtttt gcaatttctg
    42901 ttaatttatc aatatttttt cgagagtact tgtccgcctt atctttgaca acaatcgctt
    42961 taactactga agcttctgag aacaccttga agtcaacttc tttgacaatt tctgtcaagt
    43021 cttgtaaaag catatcaaac cttgtatcag gcttatcaga cccgtagtta ttcattgcat
    43081 catcatatgc catccttggg aatggtaaag aaacctctaa acctttagtg tctttcatta
    43141 ctttagcaat cataccctca acaatgtctt gaatttcttg atcactaagg aatgatgtct
    43201 ctaagtcaac ttgtgtaaac tcaggttgac ggtctccacg taaatcctca tcacggaaac
    43261 atttgacaat ttgataataa cgatcaaatc ccgcattcat caatagctgt tttgtaattt
    43321 gagggctttg tggaagggca taaaaatgtc cttggttgac acgtgatgga accaaatagt
    43381 ctcgtgcccc ctctggagtt gattttgtta gcatcggagt ttcgacatca ataaactcta
    43441 agttgtcaag gtaattacgg attgagtgtg ttactttcgc acgtaatttg aaattttcaa
    43501 gcatttccgg acgacgaaga tccaagtaac ggtaacgcat gcgtgtatca tcatttgcct
    43561 caataccatc tttaatttca aatggtgttg ttttcgaggt attaagaatc gataattctt
    43621 gcacttttaa ttcaacttct cctgttggta aattatcatt agcttgttct cgtgctgtca
    43681 ctacaccgct aacctctata acaaattcac tacggagact ttctgcggtt gccataactg
    43741 aggctgatac ttcttcagga ttaataacta attgcatgat tccctcgcga tcgcgaagat
    43801 caataaaaat aaggcctcca aggtcacgac gacgaccaac ccatcctttt aaagtaatcg
    43861 atgttcctat gtgctcacta cgaacacgcc cagcatacat actacgtttc atctgttttc
    43921 tccatttaaa atattcattc caacctatta taacaaaaag cacctaaaaa ccccacccat
    43981 taaggagagt ttttaggaac tgttttacaa tcctaattgt ttaagcacag atgaaaaatc
    44041 tgttttaatg ttttcaaaac taactttaac ttcttgacgt gtttgattat tttttaaacc
    44101 gacctctttc gaatctactt cactgctacc taatgtcata atcactttag cattaaaagt
    44161 atctgcagac ttaaattgtg ctttgatttt acgacccaaa taatcacgct ctactttaaa
    44221 accctgatga cgtattgatt gtgccaaatc taaggcagct agattagcct ctgatcctaa
    44281 tacagctata tacagatcta tagtattttc tattggtaaa gaaatccctt gcttatctaa
    44341 tattaagagc aagcgttcta atcctaatcc aaaaccaaat gctggtgttt ctggtccacc
    44401 aaaataggat actaagccgt cataacggcc tccagcacag atcgttaatt cattatcttc
    44461 aatctcagtg ataaactcaa aaatagtatg actataataa tcaagaccac gaaccatatt
    44521 agtatcaatg atgtatggaa tatttagagc atcaagcata tgacacacag catcaaagtg
    44581 ggcttggctt gactcatcta aataatctaa aatagaagga gcattttcaa ctgctagttt
    44641 gtcttctggc tccttagagt ctagtacgcg caatggattt tcattcaaac gacgttgact
    44701 atccttagac aattgttcac gtatgggtgt taagtaatca ataagagctt ggcgataggc
    44761 ctgacgactt tctggattcc ctaaactatt aagatgaagt ttaacgtttt tgatacctaa
    44821 atcttcaaat agatgatgtc ccattgcgat tgtttcaaca tcagttgctg gattatttga
    44881 tccaaagcac tcaacgccta cttgatgaaa ttctcgtaaa cgaccagcct gtgggcgctc
    44941 atatcggaac attgatccaa tataatacat tttggttggt ttttgaactt ctggtgcaaa
    45001 tagtttattt tcaacataag aacgtacaac aggtgctgtt ccttctggac gaagcgtaat
    45061 gtgacgatct cctttatcat gaaaatcata catctctttt gtaacgatat ccgttgtatc
    45121 cccaactgag cgactaataa cctcgtaatg ttcaaacatt ggtgtacgaa tttcatcata
    45181 atgatattgt ttaaagagat tgcgtataac attttcaacg tattgccact ttgctgattc
    45241 tccaggtaag atatcctgag tcccttttgg tttttgaagt ttcataagtt tacctcttcg
    45301 attagttttc tctttaaaca ttctaccatt ttttcttata ttttaaacaa gcatatatca
    45361 tttgctttag tttgatagta ttttcaccgt gaaattgtaa aaataaaaag ttttgtcaag
    45421 taactttact ttacaaaaaa gatatgttat catagtgaag ttgatgaaaa tcaaaactta
    45481 cacatcgaaa ggaagaataa gaaaatggca gtacctgcac gtcacacttc aaaagcgaag
    45541 aaaaacaaac gtcgtacaca ctacaaattg acagctccat ctgtacaatt cgacgaaact
    45601 actggagatt actcacgttc acaccgtgta tcactcaaag gatactacaa aggacgtaaa
    45661 atcgctaaag ctaacgaagc taaataatag aagggagata ccatgcgcgt aaatattaca
    45721 cttgaacata aagaatctgg tgaacgcttg taccttactt caaaaaacaa acgtaacact
    45781 ccagaccgtc ttcaattgaa aaaatactca ccaaaacttc gtaaacacgt tgttttcact
    45841 gaagttaaat aagacatcat acagaaagcc tatgaaatca acgtttctag gcttttttgc
    45901 ttgcttaaaa tgtggttttg tctgattttt gtctgattaa aagtatatta tctattcaaa
    45961 catctcctat atccgtcata cttccaagtt tttatggaat cactttaaag caactttttt
    46021 ccacttgcga atcttagttc taaatgttcc gaaaggagct accgtattaa tatgaataaa
    46081 cttataaact tcccagactg cattcttagt cgcactatca gcccaatttc tcatatgagg
    46141 tttaaatagg acttcgtcgc tcattgaatc aatcatgtta cctatagcat ctacattatc
    46201 attgagttga gccgtaagtt ctttgatggt tagactagcg taggtctcag taaaatgctg
    46261 atatagctct ccaagttgat tccatttaaa cgtctcagtt ggtgttttaa cttctaagcc
    46321 actctgttca tctgactccc attttagtat cagggtagtc cagccaacct gataggctag
    46381 attctccgct ggtgtcctat caacctcatc aattcttaaa tctttcaact tctcaggaat
    46441 gtcattgaat tcagcaatat attttttata tgattttagt acttcttcct taagttcgtt
    46501 tttatctgaa tatgttctca tttctttcct ctttaattta gtcaagttca ttgtatcata
    46561 ttcttttctc tttttcttag gaagtacagc ttttataatt ctatatatca aatgaatttt
    46621 attcaatcaa cttttctagc ttttcaactg cccctctcat ggtatctttt tgcttcttaa
    46681 tcacatggag atagacacgc tcaatctcct tggaatcctc cgagtgtccg acaaattctc
    46741 gaatagtagg gaggtcaata ccttcgctag ccataataga gataaagcta tggcggaaca
    46801 tatgagggat gacatgaatc ggtctgccaa agtctagaaa gctatcgata tccttatagg
    46861 gcttgcctgc tttcttgtta tactttgctg actgactaga gccacctctc aggaattgtg
    46921 agagttcgtt gtgataaagc ggagagcctt gtcgattacc tgccctaata taggttctag
    46981 taaagagata ccctaagtct tggtaagtcg gactgaattg agcttgaaat ttatttctgt
    47041 caatatagtg acgtagaatc ttctcaacct cagggctcat tggaatcgtg cggacacttt
    47101 ccttggtctt aaggtctgct cggtagcgtt ccttttcgtc aagattgagt gcttccatca
    47161 tatcatccga tttattctta cttgccctct gccaatgaac ttttatccac tttttctcaa
    47221 aatcaagcat gtcttcattt aagcctaaaa cctcacttgg tcgcatgcct gttagaaaaa
    47281 tcattctcaa tacatctgcg ataagttggt tatcaggtcg attagtccag ctctctatca
    47341 ggcggagaac atggttaacc tcacttgctt ctagatactt ctctgctata tcttcacgct
    47401 tcttgtcctg ctcctcactc tttgtcagaa ctcgcctttt aaccttattg ctggcaatcg
    47461 ggtcttgtgt aagagttatc tgtccatttt caactgacca gtcaaaaatc atcttaaggt
    47521 agatatgcag atttctcgcc atgtgcatag aagaagcatt gataagattt tgaatttcat
    47581 ctgtcataaa ggcaaatttc gtgatggaca ttctggtatc aaagttcggc tcaatatagc
    47641 gattatagat agtaaccctt gttttaaggg tattattggc tggcttgacc ctttcatgcc
    47701 atgctttaaa ggctttaccg taaaaatttc catctttatc aaatggcttg gcgatttctt
    47761 ctagggttaa aagtttcttg gctttcttag gcttaatgcc cattagctcg gcgatatagt
    47821 catcaactaa tttattcagt tcttgcgtga cctgagtaat taattgtgtg tttttaatat
    47881 cgctgaccaa gagacgttct ttcccagaag gtaacttttt gctcggtgtt gccttggtgg
    47941 taaaccaccc tttactagca cctgtagaaa ggtatttctt atgagatttt ttagtaatag
    48001 ggtgggtgaa tcgcatagcg aatttcacca agacattacc cgccttataa gggtctggtc
    48061 gccaagaact gatgataagt actcttgaac cattatctct catagggact acctacgcca
    48121 tctttgcgac atactgattg gcaattttgt agtcaaacca cgccacaaag acttctgagt
    48181 ggataaagac attgccgtgt gttcctttga taccgaaagc gtaagggctg tctttcatct
    48241 gacgaatgta actattaagt gttgcaacgg tcatgccatg ccagagctca tctagaaaga
    48301 cggatttgcg gtaccagtta cgggttaagt caaaatcatg ttctgttttt aatgttgcca
    48361 ttgaattctc cttctgtttt agttataatc aagttagtta tttttggaaa ggcgatttgt
    48421 agcttgctac aggtcgtctt ttcttatgcc ccaataggct gagcttagtt gctcttgttt
    48481 gtactgctga atgtcagtct cgtctgtttc ggttagctta gaaatcccca attcaagtaa
    48541 gtgctggata tagctatctg ccttttcttg cccgtgagat tccgccttag cgacatagag
    48601 ttttccgagt gttcgtgaga cttgtttctc tgtccacttc tcagaacgtt ttagggtagg
    48661 ttttgtccgt tttacgctta agaccgtctt ttcttgtgtc tctagatagt cattccacca
    48721 ttttaccgtc tttcttcgcc aacggttaga atccttgtcc ctcgggctga caaaacggta
    48781 gtgtccgtta ataacttcaa agtaaatctc tttgagcggt cgcccctctt taatctgttt
    48841 ggctagtaaa tttgcttttt cctgccagca cctcagctct gctcttgtcc atgataaggg
    48901 cgtatttggc aattcttggt ctagcttttg ttctaacaat ttgttataga cacaccattg
    48961 ctgagtacct ctaacaccaa tcgtaaccat ttcaccgacc ttttcaccat tttcaagaag
    49021 gctcttttca tggtaatcaa aggttttagc ggttgatatg cagagttgct ttttacaata
    49081 actgtaaatg agtgggacgg atagactgtc atcaaagata tcgttggcaa tatctagtct
    49141 cgtaacgtta ctatgacatt cagaaatgcg tttcatcaag gttacccagt tattgacatt
    49201 gttttccata tattcttcat actgacggca cccctgccct ctcagttcga taaagactcc
    49261 catctttgat tgccagtcct tattgaagta aacctttatc tctgagaagg aataatgccg
    49321 ttgatacttg tttatccccc attcattgag gacaaacttg tctttaggaa gaatgaggat
    49381 tttctcaatg acatcatctg gagccaagcc tttaatcgtt acggctaaat agtctaacat
    49441 cactgagtta tccatgtcta ggctccaata aatttaatat cggttgccac tagctttaag
    49501 ccaccccaat tccctccacc attattgttg gctcgccaac caagatggac ttctggattg
    49561 acaagttcta ccacaaggga gtttaaccca tccttttcaa cggatttagc caacttgtca
    49621 agattgtcta caatctccaa gcgaatgccc ttaaggtcat cagaagaata gcccaagtct
    49681 ttcaaggctt gaattttgtc tgtatcttgt acagagcaag tgattttagc cacttcatct
    49741 gtcgcaatgc tttcgccatt gacattttta tagactggtg caacccgtga agaaaggata
    49801 gtcactttct ttggaatgtc aaattgtgat ttatctaatt tcaaatcacc aacttttatg
    49861 gtttcgtctg ttaagttaaa ttttgtcatg tgtttttctc ctgttcataa aagctacgcc
    49921 tacatgaaat ctgtcataaa tcgttgatat catgggcttt ataaagattt ttttcgtgaa
    49981 atttgcttca aatcgctgaa ccccttgctt atcaagggtt ttgaccacat ttcgtgtatg
    50041 tgtgcacccc ctaataggga acggggtgca atttcaattt cgatttcagc cgtttgccac
    50101 cttccccacc tgctctgggc taccgtattg accactcgcc cagcggtggg gtggcgaacc
    50161 gtcttcatcg tttgaagaaa ccaattctct tagatgtttt atcccctgtc tctttctcta
    50221 agttctgaat tctgacaaga agcacttcta acttctgttt caactcagtt aggtttcttt
    50281 ggtaatcctc ttgaatttcc ataaaagaaa cttctagctc gtctacaagc tctctaatgg
    50341 gtttctgatg gtcttttccc ttggaactcc cataaacgct tagaattgcc ctctcaagcc
    50401 ctaatgagga cttcttatcc gcgaccgctt gaaagtaggc aaggtcatca tctgtaaaga
    50461 gatagatttt agaataagat ttccgacccg ttctcactct actttcttta aattgagtac
    50521 cagtcaagct ttcaattttt aatcgccact ttttcagtgt tgaaactgag ttaagaccgt
    50581 caaccctatc cacaacttct tgataatcat attcattcat tagcttacct gtgttttctg
    50641 attgagaaag tcggtcacaa tctgcaaggc tctagattca atcaatcgtt caaatcccgt
    50701 actcttgata aggtgataga gttctctgtc atctaaatct ttgcagtcct ctggtttgat
    50761 gaaaactttc tcttgtgtct ctggcgagta ggcaacccac tccaccattt gtagttcaat
    50821 acttgtttga ttcataatta ccctcttttc tgacgtttgc atccaactag catttatccg
    50881 aaatatctgg tataattaca gtaattagat ttactgtcct tgaacgatgt accagattgt
    50941 tcaaggattt tttctacctt cctttctttt ttgattgtta agttagctcc tttctatcaa
    51001 taatatacga ttttatatac gattgctgta aattttttga acaatcctaa tgctcattaa
    51061 aaattatata cgaaaacata tacgatgtca agtattaacc acaaaaaaat gtacgatttt
    51121 ataaactact atgttataat ggtctgagag gtaacttatg tttgaaatac ttaaacctga
    51181 taaaaaggct gttgggaaaa gattgaggca agtcaaggat gagttaaatt tgtcttttac
    51241 agattttgga aatcgtcttg gactaaaaaa atcaaccatc aacgcatatg taagaggaga
    51301 taacttagcg cctcttgaag ttttagaaaa agtatcaaaa atctatggaa aacctgtagg
    51361 ttggttttac tatggagaat tggaagaata tattgaactt taccttagaa aacttggtta
    51421 tggtacattc ctagatgaat atcctgagac accgttaaag ataaaggatg agctttttag
    51481 gattaaagca aatccaaaac aattaatcac aactaacggg ggagtaacca ttggaacatt
    51541 tgagaatcct aattgggaaa atgactttgg ttacccaaat gaaggctacc tagatgatat
    51601 cttttttgaa atgctttgtg aaactctaaa gaactcaatt agacaggaag ctcaaaccta
    51661 cctaagtcag caagaaggac ttgacgatac taaaatcaaa gaactctcta attactttgc
    51721 caaccaaatc tacatctcat atgattttgt cggaaattta gaaaatcttt ctaaagataa
    51781 aatcaagcaa gaagttaaag aaagctatga acgtctgctt aaagattcac ctcaagcagg
    51841 tgtagaagtt agctatgatt ccaattacct acttggtttt ctaataaata cacttgcgga
    51901 tagtcaaaca acaaggcaat tactttcaga tatgtcacaa gcaacagtcg gtatgccttt
    51961 ttcacaattt catgagggac acgaattagt tgaggttatc caatctgtca gaccacggct
    52021 gatagaactt tacaataaca cgacctttga tgattggact gactgggcgg agaaatgatt
    52081 ttagaaagga agatgcaaat ggataacaaa ttcaaaaata tcccaaaacg gcatttacca
    52141 caaaaactcc catttgacta cacgtcaata tatactgacc cacaaatcat taaattaact
    52201 actagggcaa atattgcaat cggtacttac gaaggatttc tagagtctat cattaaccct
    52261 atgctactaa tctccccttt gcttagccaa gaagccgttc tctcttcaaa acttgaaggg
    52321 acccatgcca ccttgaaaga cttacttaat tatgaagctg gtaataaagt agatattgag
    52381 cgtgatgaat tgcatgaaat cattaactac cgaaaggctc tcttttatgc tctagaaaac
    52441 atttccacca tcaacaacat cgattctaaa ggattaccgt tatccaatag aattattaaa
    52501 gaaatgcaca aaattcttct agataatgtt agaggtagta gtaaaaatcc tggtaatttt
    52561 aagagaagtc aaaactatat cggtagtgta tcctcgattt catacactcc tgtccccgcg
    52621 gaaaaaacac cagaatacat gtctaatttg gagcaatata tccattatga tgacttagac
    52681 ttattggttc agtcagcaat tatccatgct caatttgaaa tgattcaccc ttttgaagac
    52741 ggtaatggac gaattggacg attgcttata ccactattcc tgtactatca ggaactactt
    52801 tcttatccaa cattttatat gagttcgtat tttgaaagag ataggtctct ttacatatca
    52861 cacctatcaa acatttcaaa agacaacaac tggaaagact ggtttgagta ttacctagaa
    52921 ggagtgatat tgtccgcaga agagagtaca aaaaaagctc aggacatact aagcctatat
    52981 aacattatga aggaacaagt catccctaaa cttaattctg tttcaggtat ccagcttctt
    53041 gactttatat tctcggctcc aatctttaag gcagaacaag ttagtgaaca cctaaaaatt
    53101 tctaagagaa ctacctatac tttactaaat aagttaattg atgaaggtta tctttcaaca
    53161 gataatgctc aaaggaatag aacctactat tgtcctcaac ttctttcaat cgttcaataa
    53221 ggtatctctt aatgaagtaa tgttcacctc tgcttcactt atttcttttt tagtgaagca
    53281 aaagtcatct ttacttctct tatctatttc ttaatgaagc gaaacctgtt ttggcttcac
    53341 ttgtttttta ttatgtgaag cagagttgga tttcgcttca cttagcgact ggtaagtgac
    53401 ttatcattta tactttattt ttatactgaa taaaaggaat tttacccatg cccaagaata
    53461 ggataaacaa tttcatcaat ataggaggct taatcttagt aatctctttt ctactattag
    53521 tccttgatat agttaatctt ccaacgaaat ttggttttaa tataaagact ttaaatattg
    53581 aaatttggaa tatttacgct acgcttttag gaagcgggtt aactctgtat ggtgtaatta
    53641 taacaattaa cagtcagtat gagtcacaga tagaggatac taaacggatt gtaaagccta
    53701 tgattaatgt gtcacct
//
LOCUS       DATRTN010000008        49491 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.008, whole genome shotgun sequence.
ACCESSION   DATRTN010000008 DATRTN010000000
VERSION     DATRTN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49491
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.008"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..299)
                     /locus_tag="VBE22_000459"
     CDS             complement(<1..299)
                     /locus_tag="VBE22_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO7201935.1"
                     /translation="MAEIPNSWSYVKFGSIVTFNIGKTPPRNEPTYWGNDIPWVSISD
                     MPSSGHITKTKECISHLAIKQTNIKIVPADTLLMSFKLSIGKVAILDVPASHNEA"
     gene            complement(399..1196)
                     /gene="psrA"
                     /locus_tag="VBE22_000460"
     CDS             complement(399..1196)
                     /gene="psrA"
                     /locus_tag="VBE22_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEO7201936.1"
                     /translation="MKDFELHLRKAGLAENTIRSYLYGVRFFLEHYELKIEDLFEYKG
                     YLLDNFKPKTVNLRLQGLNKYLVFIGHDDLKLKFVKIQQKPFLEDVISHADYLFLKRS
                     LKKDDNLKWHFVVWFLGATGARVSELIKLKVEHVEVGYFDIYSKGGKMRRLYIPKKLR
                     DSCQKWLDTENRQSGYLFLNKFSQPITARGIAQQLKNYAVKYQLNTKVVYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQAIIDRVVNW"
     gene            complement(1245..2777)
                     /locus_tag="VBE22_000461"
     CDS             complement(1245..2777)
                     /locus_tag="VBE22_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO7201937.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQDPTDEPASELLKRIKAEKENLI
                     ADGKIKRDKKETELFRGADGKPYEKLADGTIQEVEVPYEIPESWNWVKLRNIGSITSG
                     GTPKSSEPSYYGGNITWITPADMGKQQNNKFFAKSSKKITELGLQKSSAQLISKNSIV
                     YSSRAPIGHINIVTEDYTTNQGCKSITPLLVDLIFLYWLLQFRTKDIILRSSGTTFKE
                     ISASGFGDTLLPLPPLAEQKRIVAQIEKALAKVDEYAESYNKLQQLDKEFPDKLKKSI
                     LQYAMQGKLVAQSPDDEPVEVLLEKIKAEKQKLYEEGKLKKKDLEELVVTKGDDNSPY
                     KEVPYNIPKSWEWVRLGNISSLNFFSSISGDKIPNDSWVLDMEDIEKETGRLVRKNYK
                     TEKSSYKSNKISFSKDTILYAKLRPNLKKVIISDENGFATTELLPIKVFGNISLDYIR
                     YCMISPFYYFNIIQSVYGVKMPRVSSGFLNSTLLPLPPLTEQQRIVSKISQVFSVIES
                     LV"
     gene            complement(2777..4240)
                     /locus_tag="VBE22_000462"
     CDS             complement(2777..4240)
                     /locus_tag="VBE22_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEO7201938.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREL
                     VWELEEDDYESIIPEDLKWRNWAHAEKGEQVLTGDDLLDFVNNRLFKELKELEITPNM
                     PIRKSIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQS
                     AGNSGEFYTPRAATDFIAEMLDPKLGETMADLACGTGGFLTSTLNYLSKQRKTSEDIQ
                     KYNQAVFGIEKKAFPHLLAVTNLFLHEIDDPKIIHGNTLEKNVREYTDDEKFDLIMMN
                     PPFGGSELDTIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKEKLVEEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEQTWFYRLDMPEGYKN
                     FSKTKPMKSEHFNPVREWWTSREEILEGNFYKAKSFTPSELADLNYNFDQCGFPKEEE
                     EILEPLELIQNYQQERAALNQKIDAVLADILELLEEN"
     gene            complement(4253..6577)
                     /locus_tag="VBE22_000463"
     CDS             complement(4253..6577)
                     /locus_tag="VBE22_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO7201939.1"
                     /translation="MATFIKDKKSMSEEDIKANFITPAILAKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYALYYQFGQRIAIVEAKDNKHSLRAGLQQAIEYGEILDV
                     PFVYSSNGNGFIEHDRITRQERELTLEEFPTREELFERLISEKEISSEVSKAISVPYY
                     TDAFSNKTPRYYQQIAINRTVEAVARGENRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITAKLLNSPEKLNSFEIYLGLYQQLTGE
                     DGEETHYQKFDKDFFDLIIIDEAHRGSAKEDSNWRQVIDYFASATQIGMTATPKETKE
                     VSNIDYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLNIDVDGYRPENGKTDAAGKLIED
                     RYYGRKDFDKTIVIDDRTQKVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAAFVK
                     ENPDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSKFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPAYVFD
                     PKGPTGGGGESTGGGEPIEKYRVTDKKVDVLNSTIQVLDENGKLVTESLTDYTRKNIL
                     GAYATLTEFITAWQAADKKRVILEELYKKGVYLDAIREAEGISEQEIDDFDLLLKLAY
                     GQKELTKSQRISRVKSSGYLQKYSEEARAVLETLLDKYMDKGIGELESIETLKLPEFQ
                     IYGGTFKIINTYFGNKQAYLRAIKELEEELFTVA"
     gene            complement(6921..7277)
                     /locus_tag="VBE22_000464"
     CDS             complement(6921..7277)
                     /locus_tag="VBE22_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO7201940.1"
                     /translation="MKNRIKNIVKQVFLTEDENKRLLALMANEKESNFSAFARKKLFN
                     QEIESWTINFPEYEQLTERFIQIGRSINRIAKLSTQVGNISQQNFIELGQLITQLLDE
                     LEKGLSVNLKKKSTKH"
     gene            complement(7274..7639)
                     /locus_tag="VBE22_000465"
     CDS             complement(7274..7639)
                     /locus_tag="VBE22_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO7201941.1"
                     /translation="MVERYRTVLKKFYITKSQNQTLNYLISYTGLRNFSNYARKMLFK
                     KFPIVLVFDETSFEDLIFSLRRIKNNINQLARIAEQSQDLQALRAMTYSVQMIEKYEK
                     SFLKYHKTKKARLLSKVDE"
     gene            complement(7632..7922)
                     /locus_tag="VBE22_000466"
     CDS             complement(7632..7922)
                     /locus_tag="VBE22_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201942.1"
                     /translation="MTIYYQLENMLVAGFKSEEELQRYQGLKAEYEEETLDYSFSIRE
                     IVNQLEIIIQSRENDFPNLEEGLLQDYQELVEYLREYDVSQAEKYTRRIYCG"
     gene            complement(7933..8787)
                     /locus_tag="VBE22_000467"
     CDS             complement(7933..8787)
                     /locus_tag="VBE22_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEO7201943.1"
                     /translation="MSEVQWIKITTDIFDDEKIQLIESMPEGDTLIVIWFKLLVLAGK
                     QNYSGVLALGDKVFYTEEMLTTVFRRKSTTVKLALKTFEQFGMIAFVDGAVTIPKWEK
                     HQNIDGLEKIRKQTRERVARHRQKQKALVSGQDSLSIECNVTETLPVTESNGQVTQQI
                     KNKNQKENKNVLDNSSRETEDQSTTYLKPEYYFLLQVIADRYNDRFIYPYNYTLTHKQ
                     KMAIGQFLSEGYMTSDEVLGMIDRIPNDVESPLAYLMSSMERLKDERLLEAKVIAHEN
                     ARKFYQKG"
     gene            complement(8799..9083)
                     /locus_tag="VBE22_000468"
     CDS             complement(8799..9083)
                     /locus_tag="VBE22_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO7201944.1"
                     /translation="MSEEATLVVQLPKVLEKQLRAHYDEMVSTAIERALEDKELYKPM
                     VRMAALSRWLDVSTTTLQKWTREGMPTMVIDGVTLYDKRAVTRWLKQYER"
     gene            9230..9748
                     /locus_tag="VBE22_000469"
     CDS             9230..9748
                     /locus_tag="VBE22_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7201945.1"
                     /translation="MTNHIAKLIEESGKKLTEISSATNIAYPTLSGYNQGIRQPKKGN
                     AQKLADYFGVSMAYILGLDDNKQAPSTMNIISDSFKISEISSVTPFKSDMEKLKKGIE
                     LGEIHLSMPLNDGFSDDFRRILARYLVNNEEEFMKTFTKHMNSLKRESEIWQTWIQTE
                     EYRIRQEDRHKK"
     gene            9803..10957
                     /locus_tag="VBE22_000470"
     CDS             9803..10957
                     /locus_tag="VBE22_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7201946.1"
                     /translation="MSVHKYQSTKGITYFVKVYLGLNDYGKKKYYTKRGFKTRKAAKA
                     HETAVNHQLNSGTFVHITAQTTYTYQELYQRWYEAYKDTVEATTAVKTADLYRLHILP
                     VFGEKKISIISPLDCQTFITDKAKTFKNMKQIKSYTSKIFEFAINMNYIERNPMSKVI
                     MPKLKKTVSENYWTVNELHHFLTIILENEPYKHYALFRLLAYSGLRKGELYALKWEDF
                     DSENQLLTVSKSLGRIDGHAIEKGTKNTFSVRSIYLDDETCSILNKWKQETSREKWQL
                     SVQPLSLDKEFMFTYCNRDGEIEPLHADYINNILKRIIRKHNLKKISPHGFRHTHATL
                     MIEMGIDPVNTAKRLGHASSQITLDTYSHATKAGEKQSITKFAEYLNKAK"
     gene            complement(11103..11495)
                     /gene="rpsI"
                     /locus_tag="VBE22_000471"
     CDS             complement(11103..11495)
                     /gene="rpsI"
                     /locus_tag="VBE22_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEO7201947.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITINKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVVGGGYAGQSGAIRHGISRALLEVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(11516..11962)
                     /gene="rplM"
                     /locus_tag="VBE22_000472"
     CDS             complement(11516..11962)
                     /gene="rplM"
                     /locus_tag="VBE22_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEO7201948.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKASDKIYYTHSMYPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            12263..13429
                     /locus_tag="VBE22_000473"
     CDS             12263..13429
                     /locus_tag="VBE22_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSag9 family transposase"
                     /protein_id="HEO7201949.1"
                     /translation="MTKHKHLTLSDRNDIQSGLDRGETFKAIGLNLLKHPTTIAKEVK
                     RNKQLRESTKDCLDCPLLRKAPYVCNGCPKRRINCGYKKTFYLAKQAQRNYEKLLVES
                     REGIPLNKETFWKIDRVLSNGVKKGQRIYHILKTNDLEVSSSTVYRHIKKGYLSITPI
                     DLPRAVKFKKRRKSTLPPIPKAIKEGRRYEDFIEHMNQSELNSWLEMDTVIGRIGGKV
                     LLTFNVAFCNFIFAKLMDSKTAIETAKHIQVIKRTLYDNKRDFFELFPVILTDNGGEF
                     ARVDDIEIDVCGQSQLFFCDPNRSDQKARIEKNHTLVRDILPKGTSFDNLTQEDINLA
                     LSHINSVKRQALNGKTAYELFSFTYGKDIASILGIEEITAEDVCQSPKLLKDKI"
     gene            complement(13744..13866)
                     /locus_tag="VBE22_000474"
     CDS             complement(13744..13866)
                     /locus_tag="VBE22_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7201950.1"
                     /translation="MKNLRLKTARASMDLLQQSLAEKVGVSCQTIAAIEKGGYN"
     gene            complement(14404..15264)
                     /locus_tag="VBE22_000475"
     CDS             complement(14404..15264)
                     /locus_tag="VBE22_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO7201951.1"
                     /translation="MTFKILTDSTSDLDEKWAQEHNVDIIGLTIELDGKTYETVGDEK
                     ITSDFLLERMQEGAKPTTSQINVGQFEEVFSTYAENDHALLYLALSSHLSGTYQSATI
                     AREMVLDKYPDAQIEIVDTMAASCGEGVLAMLATKERQEGKSLEEVKQKIESLLPKLN
                     TYFLVDDLNHLMRSGRLSKGAAIIGSVAKIKPLLKLDSEGKLVPFAKTRGRKKGIKEI
                     VTQATKTLSYSTLIIAYSGEKDSAQVMKEQLLADERIEEVIIRPLGPVISAHVGSGAL
                     ALFSLGEENR"
     gene            complement(15357..15875)
                     /locus_tag="VBE22_000476"
     CDS             complement(15357..15875)
                     /locus_tag="VBE22_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEO7201952.1"
                     /translation="MKKHSILLVDGYNMIAFWKDTRQLFKSNRLEEAREVLLRKLNHY
                     AHFEHIDIICVFDAQYVPGVRQRYDQYKISVIFTEEDETADSYIERAAAELNQSVLNL
                     VSVATSDLNEQWTIFSQGALRVSARELEQRVATVKSDLDKMSSQIDLSTPKLRPWNDE
                     QLGKLKDFLDGM"
     gene            complement(15872..16627)
                     /gene="rlmB"
                     /locus_tag="VBE22_000477"
     CDS             complement(15872..16627)
                     /gene="rlmB"
                     /locus_tag="VBE22_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEO7201953.1"
                     /translation="MTMKDKQFKEESSDLVYGLHAVTESLRANTGNKLYLQDDLRGKN
                     VDKVKALATEKKVSISWTPKKILSDMTNGGVHQGFVLKVSEFVYADLSEIMTKAENEE
                     NPLILILDGLTDPHNLGSILRTADATNVTGIIIPKHRSVGVTPVVSKTSTGAVEHVPI
                     ARVTNLSQTLDTLKDKDFWIFGTDMNGTPSHKWNTKGKLALVIGNEGKGISHNIKKQV
                     DEMITIPMNGHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(16730..17116)
                     /locus_tag="VBE22_000478"
     CDS             complement(16730..17116)
                     /locus_tag="VBE22_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEO7201954.1"
                     /translation="MIDVRLINGIALAFEGDAVYSLYIRRHLIMQGFTKPNQLHRKAT
                     QYVSANAQALLINAMLEENILTDEEQLIYKRGRNANSHTKAKNADIITYRMSTGFEAL
                     MGYLDMTGQIKRLETLIQWCIETIEK"
     gene            complement(17109..18452)
                     /locus_tag="VBE22_000479"
     CDS             complement(17109..18452)
                     /locus_tag="VBE22_000479"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEO7201955.1"
                     /translation="MIKIYDTMTRSLQDFIPLNEGKVNMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYCGYQVNYISNFTDVDDKIIKGAAEAGMDTKSFSDKFISAFMEDVAA
                     LGVKPATKNPRVIDYMDEIIDFVKVLVDKEFAYEANGDVYFRVSKSHHYAKLANKTLE
                     DLEIGASGRVDGEGEIKENPLDFALWKSAKSGEVSWESPWGKGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVNVDNEKMSKSLGNF
                     ITVHDMLKSVDGQVIRFFLATQQYRKPVNFTEKAVHDAEVNLKYLKNTFNLPIQENAN
                     DEELEQFVKAFQEAMDDDFNTANGITVIFEMAKWINSGHYTSKVKETLAELLEIFGIV
                     FQEEVLDADIESLIEQRQEARANRDFATADRIRDELAKQGIKLLDTKDGVRWTRD"
     gene            complement(18449..18631)
                     /locus_tag="VBE22_000480"
     CDS             complement(18449..18631)
                     /locus_tag="VBE22_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201956.1"
                     /translation="MKDYVTTQILSILGILAGACLSYLKFAQGNSLLGIIWAAFALMY
                     IVRLYKAYQKQKKEKP"
     gene            complement(18641..19225)
                     /gene="cysE"
                     /locus_tag="VBE22_000481"
     CDS             complement(18641..19225)
                     /gene="cysE"
                     /locus_tag="VBE22_000481"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEO7201957.1"
                     /translation="MGWWKESIAIVKEQDPAARSSLEVILTYPGIKALAAHRLSHFLW
                     NHNFKLLARMHSQFWRFWTQIEIHPGATISEGVFIDHGSGLVIGETAIVEKGAMLYHG
                     VTLGGTGKDKGKRHPTIRKGALISAHSQIIGPIEVGENAKVGAAAVVLADVPADVTVV
                     GVPAKVVRVHGQKDDLQIRSIEHDREESYYSSKL"
     gene            complement(19234..19986)
                     /locus_tag="VBE22_000482"
     CDS             complement(19234..19986)
                     /locus_tag="VBE22_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEO7201958.1"
                     /translation="MTSTNELDIRLRAFINAPDNFLDSIGLVNALHHSTVWASKEPYA
                     IQVDGQEVVPVFTDITDLNHFKEEQESARDMFWESRRSLDVLDEAISHGLAGLVYNLK
                     KEGDFGNSTIFYCEDMVQFMNNYTTILNQLLNEDNIVADIMDKTYLVPAFVHPREEGS
                     FDRFFPTMSTPEGKSYVPVFSNLLSFEKWYNHNDFGGAFRKAQGVILAWTIDDIYKPR
                     NGENEIDDTFGVAINPFDEQQVLVDWSDVEND"
     gene            complement(19988..22117)
                     /gene="pnp"
                     /locus_tag="VBE22_000483"
     CDS             complement(19988..22117)
                     /gene="pnp"
                     /locus_tag="VBE22_000483"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEO7201959.1"
                     /translation="MSKQVFEMIFAGKKLVVETGQVAKQANGSVVVRYGDSTVLTAAV
                     MSKKMSTGDFFPLQVNYEEKMYAAGKFPGGFNKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSFDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVAYVDGNFI
                     INPTAQEQEASALELTVAGTKEAINMVESGAKELSEEIMLEALLKGHEAVCELIAFQE
                     EIVTAIGKEKAEVELLQVDPELQAEIIATHNIALQAAVQVEEKKAREAATEAVKEVVI
                     GEYEARYAEHEEYDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRRVDEIRPLDAVI
                     DFLPQVHGSGLFTRGQTQALSVLTLAPMGEAQIIDGLTPEYKKRFMHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIEGITPQILEEALAQAKKARFEILDVLHGAIAEPRPQLAPTAPKIDMIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDEEGNVSIFSSDQAAIDRTKDIIASLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTANVADVLEIGEEVDVKVIKI
                     DDKGRVDASMKALLPRPPKADNPKKES"
     gene            complement(22498..22767)
                     /gene="rpsO"
                     /locus_tag="VBE22_000484"
     CDS             complement(22498..22767)
                     /gene="rpsO"
                     /locus_tag="VBE22_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEO7201960.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(22855..24114)
                     /locus_tag="VBE22_000485"
     CDS             complement(22855..24114)
                     /locus_tag="VBE22_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEO7201961.1"
                     /translation="MRAWKGIVLILSSIVVTLVAWQNAGLSEFVVPGLALTSLSLTFL
                     LSTKFRILESYFQGIENMYFYHKVMAVFSMILLLLHKIGLGQGGHGSEFAKTIGSAGL
                     YLFLSIVFVAYFGNFLKYEIWRFIHRFVYLVYILGLVHTFLILGDRILGNTLLSLIVL
                     GYAVIGVISGFYIIFLYSRMRFRRVGYVQKVTHLNHDTTEIEIAMKRPYRYDYGQFTF
                     LKIYQAGFESAAHPFSISGGHDRVIFLTVKASGDYTKSIYKQLKVGTKIALDRAYGHM
                     LFDKDKKEQVWIAGGIGITPFISFIRENSILTKRVDFFYTFSNQDNLIYQDMLESYAK
                     ANPNFKLHLNNSSLQGRLDFSQSVFEGQPTIFMCGPTSMTSTYAKVFRQKDAKSRLVY
                     EGFSFRDSWLSIFLLKTFDKVYSNLIK"
     gene            complement(24223..25152)
                     /locus_tag="VBE22_000486"
     CDS             complement(24223..25152)
                     /locus_tag="VBE22_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEO7201962.1"
                     /translation="MRCSTKEMRLVYRDFLLQANQENKQITVLEADLSSSMSTNVLAS
                     EFGKRYINLGIMEAEMVGLAAGLAIKGYKPYLHTFGPFASRRVFDQVFLSLGYSQLSA
                     TIIGSDAGVSAEMNGGTHMPFEELGLLRLIPKATIFEVSDDIQFEAILKQTLSIDGLK
                     YIRTIRKAPTAVYEGCEDFSKGFIQLRQGKDIALVASGIMVSRAIEAADYLKELGIEA
                     SVIDLFKIKPLPEELKPLLIDQSIVTIENHNRIGGIGSALCEWLSMEKNTTVSRMGID
                     ERFGQVGQMKYLLEEYGLAVKDIVEHCKSIYKS"
     gene            complement(25149..26006)
                     /locus_tag="VBE22_000487"
     CDS             complement(25149..26006)
                     /locus_tag="VBE22_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO7201963.1"
                     /translation="MTSPEERYSSLRRFATEIRLNTLETLNHLGFGHYGGSLSIVETL
                     AILYGDIMDINPEKFKESDRNYMVLSKGHAGPALYSTLYLKGFFDKTFLHSLNTNGTK
                     LPSHPDRNLTPGIDVTTGSLGQGISIATGIAYAQKIENSSYYTYTIVGDGELNEGQCW
                     EAIQFAAHHQLHHLIVFVDDNKKQLDGLTADICNPGDFVAKFEAFGFDAVRVKGDDIE
                     AIDKAIKTFQDSNSVKPKCIVLDSIKGQGVKELEELASNHHLRPDLQQKTMLERAVIS
                     LRESLEVVE"
     gene            complement(26009..27364)
                     /locus_tag="VBE22_000488"
     CDS             complement(26009..27364)
                     /locus_tag="VBE22_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEO7201964.1"
                     /translation="MKGLLDFLVNIASTPAILVALIAIIGLVLQKKGVPDIVKGGIKT
                     FVGFLVVSGGAGIVQNSLNPFGKMFEHAFHLVGVVPNNEAIVAVALTKYGSATALIML
                     AGMIFNILIARFTKFKYIFLTGHHTLYMACMIAVIFAVAGFTSFSLILFGGLALGIIM
                     SVSPAFVQKYMIQLTGNDKVALGHFGSLGYWLSGFIGGIVGDKSKSTEDIKFPKSLSF
                     LRDSTVSITISMAIIYLIVAVFAGEAYIAKEISNGVNGLVYALQLAGQFAAGVFVILA
                     GVRLILGEIVPAFKGISEKLVPNSKPALDCPIVYPYAPNAVLIGFISSFVGGLVSMIV
                     MIVTGTTVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFVQGILISFLPIFLMPVL
                     GGLGFKGSTFSDADFGLTGIILGALNHVGGAIAIVIGIVVILIGLFGISFVGKSTHKE
                     G"
     gene            complement(27377..27661)
                     /locus_tag="VBE22_000489"
     CDS             complement(27377..27661)
                     /locus_tag="VBE22_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7201965.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILKDLGVSDVEVEHYDLGGA
                     DPSAADVWIVGRDLEDSAGHLGDVRILNSIIDMDELRELVTGICQEKGLI"
     gene            complement(27664..29700)
                     /locus_tag="VBE22_000490"
     CDS             complement(27664..29700)
                     /locus_tag="VBE22_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO7201966.1"
                     /translation="MVDNKTVVIMLVFLARKNLSLYELTVQTKFSIKVIIEQINYLNS
                     FLAKNHLPAIAHSAGRYQLLGDEKEHDKIVSLLEAEQFYLTQEERVCLIYLYSFCRRE
                     FVSNVHYQDFLKVSKNTTLSDIKMLRSKLAKRGISLTYTRAKGYSLVGDEMDKHQVAF
                     QMITQLLESPIGFWSLNYILSSWKFALSYEKLEKTVEYFYESFQLSPIQDRLEKSLYF
                     IILILCRYQRSVDRVLQGSPIVSEQLKELTTIIVTNLSQDISLSKPLDQKEKDYITLI
                     LSGCFEGEGTKDDDFFEALAKAIVDEMETVSLLNFSNKEELLQGLKRHIIPAYFRLKY
                     GLTGDSGYTQNIKEHYSDLFLLVKKALRPLEEQVGLIPDSEISYFVIHFGGYLRQSGD
                     TQSMSYKALILCPNGVSSSLVIKEKLRGLFPQIHFHRVSKIEQLKLIDNQTYDMVFST
                     IFVETKKPNYLVSLMMTAEQVQQLKELVISDFPKACLDDFQLDQLIATIKKYAHVHCE
                     EELKLALRTMVKQDIFRKDVRPLLHQLITEETYQTSSEQMNWKEAIRLAAKPLLASGK
                     ITESYPEAMIEKVEEFGPFINLGKGIAIPHARPEDGVNSVGMSMLVLEQPIYLLDDPK
                     QEIYLLLCIAAIDNETHLKALSHLTTILRDNNNVKALLASRGYQDIEMIIKQED"
     gene            complement(29920..31545)
                     /gene="treC"
                     /locus_tag="VBE22_000491"
     CDS             complement(29920..31545)
                     /gene="treC"
                     /locus_tag="VBE22_000491"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEO7201967.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLAELGID
                     MVWLNPFYPSPQRDNGYDISDYTAINPDFGKMDDFEEMIEVGRQYRIDFMLDMVLNHC
                     SIEHEWFKKALAGDRYYQDFFILRDNPTDWVSKFGGNAWSPFGDTGKYYLHLFDITQA
                     DLNWRNADVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEILENCPINDGKPAYTDRP
                     ITHDYLKMLNNASFGQADSFMTVGEMSSTTIANCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMAFDFPALRDLFHSWGEGMSEGNGWNALFYNNHDQPRALNRFVDVKRFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDYSSMDDYVDIESLNAYQIMLDEGKSQE
                     EAFSIIRAKSRDNSRVPMQWDDSTNAGFSEGAPWLKVGKSYKEINVAKEKTGLIFTFY
                     QELIRLRKQLPIIADGNYKAAFKDNEKVYAFERHLDKQKLLVLNNFFAEKVKIKLPEN
                     YLQGQVLLSNYKDVTLDETVTLQPYQTLAILVS"
     gene            complement(31767..33797)
                     /gene="treP"
                     /locus_tag="VBE22_000492"
     CDS             complement(31767..33797)
                     /gene="treP"
                     /locus_tag="VBE22_000492"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEO7201968.1"
                     /translation="MEQFKHDAKALLEAIGGKENISAVTHCATRMRFVLNDSSKAKVK
                     VIEELPSVKGTFTNAGQFQVIIGNDVPIFYNAFVAVSGIEGVSKEAAKSAAQKNQNPL
                     QRVLTMLAEIFTPIIPAIIVGGLILGFRNILDAVPFEFLGQKVVDGVRQVDSSGHPIW
                     NTLVDVSTFWSGVDSFLWLPGEAIFHFLPVGIVWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYSVASTSAADIAKNWSWNFGYFTVQKIGYQAQVIPALLAGLSLSYLEIFWRKHI
                     PEVVSMIFVPFLSLVPAIILAHTVLGPIGWTLGKWISAIVLIGLTGPVKWLFGAIFGA
                     LYAPFVIAGLHHMTNAIDTQLIADTKTHTTGLWPMIALSNIAQGSAVLAYYFMHRHDE
                     KEAQISLPAAISAYLGVTEPALFGVNVKYIYPFVAGMIGSSVAGLLATTFNVQANSIG
                     VGGLPGFLSINVKYMGYFFICMAVAIFIPLFLTLFFKKSGILTKTEEEKLVSDAVIAS
                     TTETKSAKEKAVVSGTKLSVVSPLSGLAKPLDQASDPVFSQGIMGKGVVIDPSDGELV
                     SPVDATVSVLFPTKHAIGLLTSEGVEFLIHIGMDTVNLEGKGFTSHVAQGDNVKVGDK
                     LITFDIPMIKEEGYIVETPILITNQEEFRPEELIDLPKQIKRGQALMVAKKI"
     gene            complement(34079..34705)
                     /locus_tag="VBE22_000493"
     CDS             complement(34079..34705)
                     /locus_tag="VBE22_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7201969.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEINLEVAPGQVLGIFGQSGCGKTS
                     LSKVLAGFLHPRSGEVLVDGSNLPNKAFRPVQLIQQHPEKTMNPLWPMKKSLEEAYYP
                     SRDLLDAFGIQEKWLNRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIVISHDFAMLEKLCNQCYMIEENRIVSFNGD"
     gene            complement(34689..35492)
                     /locus_tag="VBE22_000494"
     CDS             complement(34689..35492)
                     /locus_tag="VBE22_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7201970.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEIKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNASVTGDMIYRGQSLNSKRIKQLRGKDITLIPQSVN
                     YLDPSMKVKHQVRLGISENSKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDKVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGNGEQLQTEFARSLLRSLPQQEFLKGVTHDLRG"
     gene            complement(35504..36325)
                     /locus_tag="VBE22_000495"
     CDS             complement(35504..36325)
                     /locus_tag="VBE22_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7201971.1"
                     /translation="MILRRRTIVLWQLGIAISLILSILALNLYFHSTPLQTNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVILATVFGVLAGLGNSIID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYHLKN
                     KEFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(36322..37299)
                     /locus_tag="VBE22_000496"
     CDS             complement(36322..37299)
                     /locus_tag="VBE22_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7201972.1"
                     /translation="MKHITTVVIWKILRCLSLVFGVSILTFILLKQSPVDPVMASVNY
                     DTSLTPVQYKAIAHHYGLDKPAPVQYFIWLKNFIQGHLGTSLVYRQPVIDIIRSRAGA
                     SFILMGLSWVLSGLIGFILGTLSAFHQGKLLDRIVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLILPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETEWQIFKNHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLIAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(37412..39040)
                     /locus_tag="VBE22_000497"
     CDS             complement(37412..39040)
                     /locus_tag="VBE22_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7201973.1"
                     /translation="MTKYLKYISFVALFLASIFLVACQNQNSQTKERTRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGIHNEGNITHSTLLKRSPELDIKGELAKKYKISKDGLTWSF
                     DLNDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIASNDVRGLSLPYVKK
                     GVVKNSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDRTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGNLKAEFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHYPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSPDLDKANKYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESAK"
     gene            complement(39283..40011)
                     /gene="lrgB"
                     /locus_tag="VBE22_000498"
     CDS             complement(39283..40011)
                     /gene="lrgB"
                     /locus_tag="VBE22_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HEO7201974.1"
                     /translation="MELLKTPIFGICFSLILYTIGQHLFKKSKGFFLLQPLFFAMVSG
                     IVILWLMSKGLGTDVKTFYTQAYKPGGDLIFWFLNPATIAFAVPLYKKNDVVKKYWVE
                     ILSSLVIGMIVSLMLIVAISKMVGLSQVGIASMLPQAATTAIALPITAAIGGNTAVTA
                     MACILNAVIIYALGKKLVSFFHLNDSKIGAGLGLGTSGHTVGAAFALELGELQGAMAA
                     IAVVVIGLVVDLVIPIFSHLIGLL"
     gene            complement(40013..40468)
                     /locus_tag="VBE22_000499"
     CDS             complement(40013..40468)
                     /locus_tag="VBE22_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO7201975.1"
                     /translation="MKHTSKETKTKEAPMFIQMSIYAAILLVSQMISMLLPKSLPIPT
                     TVIGLVLMYVLLTAKIIKVEWVDSFGALMISMIGFMFVPSGISVAANLDILKAEGLQL
                     VAVITISTVVMLVVVAYVARLILAIRELSVTDLFKKILPNHYSKKVEEK"
     gene            complement(40638..41378)
                     /locus_tag="VBE22_000500"
     CDS             complement(40638..41378)
                     /locus_tag="VBE22_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEO7201976.1"
                     /translation="MKILILDDEMFARQELSFLVEHSQEVDNPEIFQAEDISEAEKIL
                     FRQQIDLIFLDISLSEENGFTLANQLSQLAHPPLVVFATAYDNYAVKAFESNAVDYIM
                     KPFEQQRVDMALSKVKKLSQLTTASDVEQAIPKKASVELLTLTLSDRSVVVKMQDIVA
                     ASVEDGELTVSTVQKTYTIRKTLNWFKSRAVAPYFLQIHRNTVINLEMIEEIQPWFNH
                     TLLLIMSNGEKFPVGRSYLKDLNEHLTM"
     gene            complement(41359..43104)
                     /locus_tag="VBE22_000501"
     CDS             complement(41359..43104)
                     /locus_tag="VBE22_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor protein LytS"
                     /protein_id="HEO7201977.1"
                     /translation="MLMVLLFQRLGIIMILAFLLVNNSYFRQLIEERSKRETVVLVII
                     FGLFVIISNITGIEIKGDRSLVERPFLTTISHSDSLANTRTLVITTASLVGGPLVGSI
                     VGFIGGVHRFFQGSFSGSFYIVSSVLVGIVSGKIGDKLKENHLYPSTSQVILISIIAE
                     SIQMLFVGIFTGWELVKMIVIPMMILNSLGSTLFLAILKTYLSNESQLRAVQTRDVLE
                     LTRQTLPYLRQGLTPQSARSVCEIIKRHTNFDAVGLTDRSNVLAHIGVGHDHHIAGQP
                     VKTDLSKSVIFDGEPRIAQDKAAISCPDHNCQLNSAIVVPLKINDKTVGALKMYFAGD
                     KTMSEVEENLVLGLAQIFSGQLAMGITEEQNKLASMAEIKALQAQINPHFFFNAINTI
                     SALIRIDSDKARYALMQLSTFFRTSLQGGQDREVTLEQEKSHVDAYMNVEKLRFPDKY
                     QLSYDISAPEKMKLPPFGLQVLVENAVRHAFKERKTDNHILVQIKPDGHYYCVSVSDN
                     GQGISDTIIDKLGQETVAESKGTGTALVNLNNRLNLLYGSVSCLHFSSDKNGTKVWYR
                     IPNRIREDEHENFNS"
     gene            complement(43131..43775)
                     /locus_tag="VBE22_000502"
     CDS             complement(43131..43775)
                     /locus_tag="VBE22_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7201978.1"
                     /translation="MEKVIIFDMDGVIVDSEYTFLDNKTEMLREEGIDTDVSYQYQYM
                     GTTFEFMWQAMKEEFGLPKTVKEYIAEMNRRRQAIVARDGVRPIKGAQRLIHWLHQHG
                     YRLAVASSSPMVDIKRNLKELGVTECFEYMVTGEDVSSSKPAPDVFLRAAELLDVDPK
                     VCIVIEDTRNGSLAAKAAGMYCFGFANPDYPPQDLSMADKVISDYQDIYIYLPE"
     gene            complement(43898..44293)
                     /locus_tag="VBE22_000503"
     CDS             complement(43898..44293)
                     /locus_tag="VBE22_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7201979.1"
                     /translation="MYNKVIMIGRLTAKPEMVKTPTDKSVTRATVAVNRRFKGSNGER
                     EADFINVVMWGRLAETLASYGTKGSLISVDGELRTRKYEKDGQTHYITEVLASSFQLL
                     ESRSQRAMRENNISGDLSDLVLEEEELPF"
     gene            44374..45090
                     /locus_tag="VBE22_000504"
     CDS             44374..45090
                     /locus_tag="VBE22_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO7201980.1"
                     /translation="MTNVTSYKEMLAKPWGKIQYEITFAQLSHIKNQNVLDFGAGFCL
                     TAQHLAKENNVTAIEPNPKLLYDNQSDNIYKILGSYEALRDLPNQSFDTIICHNVLEY
                     IDKHNHPAYFDEFSRLLKPNGELSLIKHNITGKILQSVIFSNDTSTAMELLTGEANFK
                     SASFDQGNIYTLEELKQNTNLLVERYQGIRTFYSLQPNHFKTETGWLNKMLAIELSVA
                     DKAPYKDIAFLQHITLKKSL"
     gene            complement(45144..45770)
                     /locus_tag="VBE22_000505"
     CDS             complement(45144..45770)
                     /locus_tag="VBE22_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEO7201981.1"
                     /translation="MIFTYNREHVGDTLMVIVKDSQGAKLDVDRRGQVARVYLQDSKE
                     TVAWNIFEVSSLIVIEGAGQITLSDQDIKILNAELLKEGFEDSLVNNIEPTFVVAQIK
                     EMIDHPDSDHLHICQVEINDGKTVQIVCGAPNASVGLKTVAALPGAMMPNGSLIFPGK
                     LRGEDSFGMLCSARELALPNAPQVRGIIELSDQVIVGESFDANKHWKN"
     gene            complement(45803..46126)
                     /locus_tag="VBE22_000506"
     CDS             complement(45803..46126)
                     /locus_tag="VBE22_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO7201982.1"
                     /translation="MILLESYEEIAAYIDSTKKVVFFFTADWCPDCQFIYPVMPSIEK
                     DFSDFVFVRVNRDDYIELAQQWNIFGIPSFVVVENGQELGRLVNKNRKTKAEITKFLA
                     EINHK"
     gene            complement(46123..46407)
                     /locus_tag="VBE22_000507"
     CDS             complement(46123..46407)
                     /locus_tag="VBE22_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEO7201983.1"
                     /translation="MKNKKILFGTGLAGVGLLAAAGYTLTKKVTDYKRQQITQTLREF
                     FSQMGDIQVFYFNEFESDIKMTSGGLVLEDGRIFEFIYRQGVLDYVEVSK"
     gene            46568..46807
                     /locus_tag="VBE22_000508"
     CDS             46568..46807
                     /locus_tag="VBE22_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7201984.1"
                     /translation="MKEKLLVPALNCLLGLLFLGDHWFLFRLQNGELFAIVTLILLCT
                     TSFLINKNHPKNLTLISMIITFLAVLSFLSTFLLA"
     gene            46992..48059
                     /gene="pepA"
                     /locus_tag="VBE22_000509"
     CDS             46992..48059
                     /gene="pepA"
                     /locus_tag="VBE22_000509"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEO7201985.1"
                     /translation="MSDLFNKIKTVTELDGIAGYEHNIRNFLRQEITPLVDQVETDGL
                     GGIFGVKNTHETNAPKVMVAAHMDEVGFMVSHIQPDGTFRVLEVGGWNPLVVSSQRFT
                     LYTRSGDAIPVISGSVPPHFLRGQSGGTTLPKISDIVFDGGFTDKNEAESFGIAPGDI
                     IVPKSETILTANQKHIMSKAWDNRYGVLMVTELLKSLKDQSLSNTLIAGANVQEEVGL
                     RGAHVSTTKFNPDIFLAVDCSPAGDIYGEQGKIGEGTLIRFYDPGHIMLKDMRDFLLT
                     TAEEAGIKYQYYAAKGGTDAGAAHLKNSGIPSTTIGVCARYIHSHQTLYAMDDFLQAQ
                     AYLQAIVNKLDRSTVDIIKGY"
     gene            48129..48899
                     /locus_tag="VBE22_000510"
     CDS             48129..48899
                     /locus_tag="VBE22_000510"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEO7201986.1"
                     /translation="MKIGIIGVGKMASAIIQGLKQTQHDIVISGSCLERSKEIAERLD
                     VTYAESHQSLINQADIIMLGIKPQLFEKVLLPLDITKPIISMAAGISLARLSQLTRSD
                     LPLIRIMPNINAQILQSCTAICYNNHVSDELRQLTKEITDSFGSSFDIAETNFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKYGFPKEQALSIVGQTVLASSQNLLQGQNSTSDLIDN
                     ICSPGGTTIAGLLDLEKNGLTHSVISAIDATIEKAKKL"
     gene            complement(48920..>49491)
                     /locus_tag="VBE22_000511"
     CDS             complement(48920..>49491)
                     /locus_tag="VBE22_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7201987.1"
                     /translation="KNHYQTPFTSILLIGLELLIIALFLYYAKVKQIIRWKALLTRKA
                     LVTIFLGWLSLRVPQIIGYLIMTMQGVKDTANQTVIMELTKQLPLALMLIFTIIGAPI
                     MEEIIFRYIIPKELFAKHQKWGFVIGTLAFALIHSPSDIGSFIIYAGMGAILSFVYYK
                     TEHLEYSIMVHFINNALAYSVLISTMMNN"
BASE COUNT        15713 a        10136 c         7955 g        15687 t
ORIGIN      
        1 attgcctcat tatgactggc tggaacatct aatatcgcaa ccttgccgat tgatagttta
       61 aaactcatca gtagggtatc tgcgggaaca attttaatat ttgtttgctt tatagctaaa
      121 tgagatatac actcctttgt tttagtaata tgaccactgc tcggcatatc cgaaatagat
      181 acccatggga tatcattgcc ccaataagtc ggttcgttcc tgggtggtgt ttttccaatg
      241 ttaaaggtaa caatcgaacc aaacttaacg tagctccaag agttaggaat ctcagccata
      301 gttgaaccga caaaatcact attttttttg ctattcctat aaggagagca accgtcacaa
      361 aaacagcaga aagtcacaaa gactttctgc tatttatgtt accaattaac aaccctatct
      421 ataatagctt gttgctctgt cgctgttttt ctaaggtaga tacgagttgt ttcgatgctt
      481 tcatgtccca tcaaatctgc tagtagggca atgtcattgt atttggcaag aaaatttttg
      541 gcaaataggt gtctaaagga atgaggatag acgaccttgg tattgagttg atattttaca
      601 gcatagtttt ttagttgttg ggcgatccct ctagctgtaa tcggctgact aaatttatta
      661 aggaaaaggt acccgctttg gcgattttca gtatcaagcc atttctgaca actgtcacgt
      721 aattttttag ggatgtagag tcgtcgcatt ttacccccct ttgagtaaat atcaaaataa
      781 ccgacctcca catgttctac ttttaatttt atcaattcac taacacgagc tcctgttgct
      841 cctagaaacc aaactacaaa atgccatttt aagttatcat cttttttcaa actccgtttc
      901 agaaagagat aatctgcatg gctgataaca tcttctaaaa atggtttctg ctggatttta
      961 acaaatttta atttcaaatc atcgtgtcca ataaaaacaa ggtatttatt aagcccctgc
     1021 aatcgcagat taacggtttt aggtttgaaa ttatccagta aatatccttt atattcaaat
     1081 aaatcttcta tttttaactc ataatgttcc aaaaaaaagc gaacaccata caggtaagac
     1141 cgtatggtgt tttcagctaa tccagctttt cgtaaatgta attcaaagtc tttcatcttc
     1201 aactcctatc tgatttttga tagaaattcc accgcacata agtattatac taaagattcg
     1261 ataactgaaa agacttgaga aattttagaa acaatacgct gttgttcggt gagaggcggg
     1321 agggggagga gagttgaatt tagaaaacca gaacttaccc ttggcatttt aacaccataa
     1381 accgactgga taatattaaa ataataaaac ggtgaaatca tacagtatct tatgtaatct
     1441 agcgaaatat tcccaaaaac tttaatcgga agaagttctg ttgtcgcgaa accgttttcg
     1501 tcagaaatga taactttctt tagattgggc cgtaatttag catataaaat agtgtcttta
     1561 gaaaaagata ttttattact tttataagac gacttttctg ttttatagtt ttttctaact
     1621 aatcttccgg tctctttttc tatatcttcc atatctaaaa cccagctatc atttggaatc
     1681 ttatctccag atatactaga aaaaaaattc aacgaagata tattacctaa ccttacccat
     1741 tcccagcttt ttggaatgtt gtacggcact tccttataag gagagttatc atctcctttt
     1801 gttaccacaa gctcttccaa atctttcttc ttgagtttac cttcctcata gagtttttgc
     1861 ttttcagctt tgattttttc aagcaagact tcaacaggtt catcatctgg actttgagct
     1921 actagcttac cttgcatggc gtattggagg atagatttct tcagtttatc tggaaattct
     1981 ttgtctagtt gttgtagttt attgtaactt tctgcatact cgtctacttt tgctaatgct
     2041 ttttcgattt gagcgacaat ccgtttttgc tcagcgaggg gagggagggg gagaagtgta
     2101 tctccaaaac ctgatgctga tatttcctta aatgttgtcc ctgaagaacg taaaataata
     2161 tctttggttc taaactgtag taaccaatat aaaaaaatta aatcaacaag tagaggtgtt
     2221 attgatttac aaccttgatt tgtagtgtag tcttcagtaa caatatttat atgtccgatt
     2281 ggagctcgac tagaataaac aatagagttt tttgaaatta gttgagcaga agatttttgt
     2341 aaacctaatt cagtaatttt tttcgatgat ttagcaaaaa acttattatt ttgctgtttt
     2401 cccatatctg ctggagttat ccaagtgata ttcccaccat agtaggaagg ttcagaagat
     2461 tttggcgtgc ctccagaggt aatactacca atatttctta atttcaccca gttccaactc
     2521 tctggtatct catatggtac ttcaacttct tggatagttc catctgccaa cttctcataa
     2581 ggtttcccgt cagcacctcg aaaaagctct gtttcttttt tatctcgctt gattttgcca
     2641 tcagcaatga ggttttcttt ttccgctttg atacgtttaa gaagctcact agctggctcg
     2701 tcagttgggt cttgaggaac caatttcccc tccatcgctc tctgtaagat actagctttt
     2761 agttgttctg gggtcattag ttctcctcca aaagctctaa aatatctgcc aaaacagcat
     2821 ctattttttg gttcaaagcc gctctttctt gctgatagtt ttgaataagt tccagcggtt
     2881 ccaaaatctc ttcttcttcc ttgggaaagc cacattgatc aaaattatag ttcaaatcag
     2941 ctaactcaga tggtgtaaaa gatttggctt tataaaaatt tccctctaaa atctcctcac
     3001 ggctagtcca ccactcacga acaggattga aatgctctga tttcatcggt ttggtcttag
     3061 aaaagttttt atacccctct ggcatatcca aacgataaaa ccaagtctgc tccgtcttct
     3121 tagtcttgtc aaagaaaagg atattggtgt gaatacctgt gtaaggtgca aagacactat
     3181 gaggcaaacg gataatcgta tgaagattaa attcctcaac caatttttct ttcaaacgag
     3241 ttttaacacc ctctccaaag agaaagccat ctggtaaaat taccccaaca cgaccgtttt
     3301 ctttcagcct atacatgata actgccataa aaaggtccgc tgtctccgaa ctccgcaact
     3361 ctgccggaaa attattttta atcgtatcaa gttcggaacc accaaaaggt gggttcatca
     3421 tgataaggtc aaacttctcg tcatctgtgt actctcggac atttttctcc aaggtattgc
     3481 catgaatgat tttagggtca tcaatttcat gaagaaagag gttcgtcact gctaaaaggt
     3541 gaggaaaggc ttttttctca atcccaaaga cagcctgatt gtatttttga atatcctcgc
     3601 tagtcttacg ctgtttgctt aaataattta aagtcgaggt caagaaacca cctgttccac
     3661 aggcaagatc agccattgtt tcacctaatt tagggtcaag catttcagca atgaaatcgg
     3721 tcgctgcacg aggggtataa aattccccag agttccctgc actttggatg tccttgagga
     3781 ttttctcata aatatcatta aaggagtgtc ggtcttctgg actattaaag tccacctcat
     3841 caatgacatt gataacctga cgcaagagca caccgttttt catgtagtta ttagcatctt
     3901 caaaggctga tttaacaatg cttttccgaa tgggcatatt gggtgtgatt tcaagctctt
     3961 ttaattcctt aaacagccta ttgttcacaa aatcaagcaa atcatcacct gtcaagactt
     4021 gctcaccttt ttcagcgtga gcccaatttc gccattttaa gtcttcagga ataattgact
     4081 cataatcatc ttcctccaac tcccaaacca actcacggct atcataaatt ttcaaaaaga
     4141 gtaaccaaga catttgttca atacgttggg cgtctccatt aacacctgcg tcattgcgtg
     4201 taatatcttg aatacgtttc acaaatgatg taattgacat agtttccttt cattaagcta
     4261 ctgtaaatag ctcttcttcc aattctttaa tggcccttag ataggcttgc ttatttccaa
     4321 aataggtatt gataatctta aaggttccac cgtaaatttg aaactctggt aattttagcg
     4381 tttcaatgct ttccagttca ccaatcccct tatccatgta cttatctaat aacgtctcta
     4441 aaacagctcg ggcctcttca ctgtactttt gcagataacc actactttta accctgctaa
     4501 ttctctggct cttagtcaac tctttttgac cataagccag ttttaacagc aagtcaaagt
     4561 catcaatctc ctgttcagaa atcccttctg cttctcgaat agcatcaaga taaacgcctt
     4621 ttttatagag ttcttctaag atgactcttt tcttatccgc tgcttgccag gcagtaataa
     4681 actcggtcaa ggtagcgtaa gctcctagaa tatttttccg agtataatcg gtcagacttt
     4741 ctgtcactaa cttcccattt tcatccaaaa cctgaatggt tgaattgaga acatctactt
     4801 ttttatcagt gaccctatat ttttcaatag gttctcctcc tccagttgat tcgccgccac
     4861 ctccagttgg ccctttaggg tcaaagacat acgctggctc tccatcaaaa tctggatctg
     4921 caaataaatt ggtcacgttt ctaaaatcaa taatggtgaa aaattctttc cctttctgcg
     4981 ggtacaaacg tgttccacga ccaataatct gtttgaactc cgtcatggat tgaatattgg
     5041 aatcgagcac aatcaaacga caagtttttg cattgacccc cgttgtcaag agttttgatg
     5101 ttgtgacaat agctggaaac ttagaattaa catccataaa gttatctagc tgtgctttac
     5161 cctcggcatt gtcgccagtc acctgcatca catagcgata gtcttcctga accaaatcag
     5221 ggttttcttt gacaaaagct gctcgcatcc gctcagcatg gtcaatatca acacaaaaga
     5281 caatggtttt gtcaaatcga gcattgttct gtttcatgta atcagaaaca aacttggcca
     5341 ccttctgagt acggtcatca ataacaatag tcttatcaaa atcttttcga ccatagtacc
     5401 tatcttcgat aagttttcct gcggcatccg ttttcccatt ttctggacga tagccatcaa
     5461 catcaatatt caaattgacc cgcatcacgc gataaggagc caggaagcca tcctcaatgc
     5521 cctgtttcaa actataggtg taaattggtt caccaaaata atctatattg gaaacctctt
     5581 ttgtctcctt tggtgtcgct gtcatcccaa tctgggttgc tgaggcaaaa taatcaatca
     5641 cttggcgcca gttgctgtcc tccttagctg aaccgcggtg agcctcatca ataataatca
     5701 ggtcaaagaa atccttatca aacttttggt aatgcgtttc ttcgccgtct tcacctgtta
     5761 attgctgata caaacctaag taaatttcaa aagaatttaa cttttctgga gagttcagta
     5821 actttgctgt gattttggtc ataacctttt caaaaggctt aaaatcttca gccattgttt
     5881 ggtcaactaa gatatttcgg tcggctaaaa atagaacacg tttggctaaa ccagcttttc
     5941 gcaaacgatg gataatttga aaagccataa aggtcttacc tgtccctgtt gccataacaa
     6001 acatgacccg attttctcct cttgcaaccg cctcaacggt acggttgata gcgatttgct
     6061 ggtaataacg tggtgtttta ttggagaacg catcagtata gtaagggact gaaatcgcct
     6121 ttgaaacctc agaacttatc tctttttcag aaatcaaacg ctcaaataat tcttcacgag
     6181 taggaaactc ctctaaagtt aactcacgtt cttgcctcgt gatacggtca tgctcaataa
     6241 aaccattacc atttgaggaa taaacaaaag gaacatctaa aatctcacca tattcaatcg
     6301 cctgctgtaa tccagctcgt aaactatgct tattatcttt agcttcaacg attgcaatac
     6361 gctgcccaaa ttgatagtag agagcatagt ctgatttttt cccttcttta cgacgagcct
     6421 tatcacctcg aacctctatt cgcccatctg taaaatattc ctcataggca atatgctcac
     6481 catttttcca acctttagct aaaatggctg gtgtgatgaa attcgcttta atatcttctt
     6541 cgctcatgct ctttttgtct ttgatgaatg tcgccatacc aagtctctcc acatctacac
     6601 ttaataaaaa attatcagaa gtatgttacc cgtctattat accattttct aacctcatct
     6661 tgtattattt ctcaaggggt ttggggcttt gccccaagtg ttaaaatcca tcaggagaag
     6721 gagctcccaa actcgttact gatggtttat tttttcgata gtgtgtatac actatctgag
     6781 ctcgcaaaga cctaaaatta tgtttttgta tcaattctaa attccccgtg ccagacacgg
     6841 tggctgacat tgtcagacaa tcatttttta gaaggacata agaaaaccgc agataaattc
     6901 ctgcggtttc atttacttat ttaatgttta gtagacttct ttttcaggtt aactgagagt
     6961 cccttttcca attcatccaa taattgagtt atcaactgac caagttctat aaaattctgt
     7021 tgtgaaatat ttccaacctg agttgacaac ttggcaatcc tattaattga tctgccaatc
     7081 tgaatgaatc gctctgtcag ttgctcatac tctggaaaat taatcgtcca tgattcaatc
     7141 tcttggttaa ataatttctt tctggcaaaa gcggaaaagt ttgactcttt ttcattagcc
     7201 attaatgcta acaatctttt attctcatcc tcagtcaaaa agacttgctt cacgatattt
     7261 ttaattctat ttttcattca tccacttttg atagtaacct cgcctttttc gttttatggt
     7321 atttaaggaa cgatttttca tatttttcaa tcatttgaac actgtaagtc atagctcgta
     7381 atgcttgaag atcctgagat tgctcagcta tccttgctaa ctgattgata ttatttttaa
     7441 ttcgcctcaa agaaaatatc aagtcttcaa atgaagtttc atcaaatacc aaaacaatag
     7501 ggaatttctt aaataacatt ttcctagcat agttagaaaa attccttaaa cctgtataac
     7561 tgataaggta gtttagcgtt tgattctgcg attttgtgat atagaatttt ttcagaactg
     7621 ttcgataacg ctcaaccaca ataaattctc cttgtgtatt tttctgcctg agacacatcg
     7681 tattccctca aatattctac aagttcctga taatcttgca acagcccttc ttctaaattt
     7741 gggaagtcat tttctctact ctggataata atttctaatt gattgacaat ttctctaatt
     7801 gagaaactat aatccagggt ttcttcctca tactctgctt ttaacccttg ataccgttgt
     7861 aactcttctt ctgatttaaa accagcgact agcatattct ccaattgata ataaattgtc
     7921 attatactct ccttatccct tttggtaaaa ctttctagca ttttcatgag caattacctt
     7981 agcttccaat aatcgctcat cttttaggcg ctccatagag ctcattaaat aggctaatgg
     8041 tgactctaca tcattaggaa tacgatctat cattcccaag acttcatcag aagtcatata
     8101 gccttcactt aaaaactgtc caatcgccat tttttgctta tgcgttaggg tgtaattata
     8161 ggggtaaata aaacgatcgt tatatctatc cgcaatcact tgcaaaagga aataatactc
     8221 tggtttgaga taagttgttg actgatcttc tgtctctcta gaactattat ctagtacatt
     8281 cttattctct ttctgattct tgttcttaat ctgttgcgtt acttgaccgt tactttccgt
     8341 tacaggtaac gtttctgtaa cgttacattc tattgataaa ctatcttgcc cacttactag
     8401 agctttttgc ttttgacgat gacgagccac tctttctctt gtttgtttac gaattttttc
     8461 tagaccatca atattctgat gtttctccca tttaggaatt gtgactgctc catccacaaa
     8521 agcaatcatt ccaaattgtt caaatgtttt cagagccagc ttcacagtcg ttgactttct
     8581 cctaaagact gtagttaaca tctcttctgt ataaaagacc ttatctccta gagctaagac
     8641 tccactgtaa ttctgtttac ctgctaaaac gagtagttta aaccagatga caattaaagt
     8701 atctccttcg ggcattgact caatcaactg tattttttca tcatcaaaga tatctgtagt
     8761 aatcttaatc cattgtactt cactcatacg tgtacctcct atctttcata ttgctttagc
     8821 catctagtga cagctcgttt atcgtacaga gtcaccccat caatgaccat tgtaggcatt
     8881 ccttctcgtg tccatttttg aagggttgtc gttgatacat caagccacct agaaagagct
     8941 gccatccgaa ccataggttt gtatagttct ttatcttcta gcgctctttc aatagctgta
     9001 gagaccattt catcataatg tgcccttaac tgtttttcca aaactttggg aagttgaaca
     9061 actaaagtcg cttcttcaga catattatta cctcgtttcc atttctcaat taaataaact
     9121 ccgaatttgt ttgttattca ttttgaatta tatattcttt tagaattttt gtcaactatt
     9181 tttattcaaa aagaattaaa tgtgttataa tactctcaag gaggtattta tgaccaatca
     9241 tattgcaaaa ctgatagaag agagcggcaa aaaactgact gagattagtt ctgctacaaa
     9301 tatcgcttat ccaaccttat caggatacaa tcaaggcatt cgtcaaccga aaaaaggaaa
     9361 tgctcaaaaa ttagctgact atttcggcgt atccatggct tacatcttag gacttgatga
     9421 taacaaacaa gcaccatcaa ctatgaacat tatttcggac agttttaaaa tttccgagat
     9481 tagttctgta acacctttta aaagcgatat ggaaaagctg aagaaaggaa ttgaactagg
     9541 agaaatccat ctgtctatgc ccttaaatga cggcttctca gacgatttta ggcgcatttt
     9601 agctcgctat ttggttaata atgaagaaga attcatgaaa accttcacta aacacatgaa
     9661 tagtttaaaa cgagaatcag aaatttggca aacctggatt cagaccgaag aataccgaat
     9721 tagacaagaa gatcgacata aaaaataaac taacagtttc tcaattacat aagctccgaa
     9781 aacttagaaa cggagaactg ttatgtctgt acacaagtac caatctacaa aaggaatcac
     9841 ctattttgtt aaagtttacc ttggtctaaa cgactacggt aagaaaaaat attatactaa
     9901 acgagggttc aagacgcgta aagctgccaa agctcatgaa actgctgtta atcatcagtt
     9961 gaatagtgga acttttgtcc atattactgc tcaaacaacc tacacttacc aagagcttta
    10021 tcaaaggtgg tatgaagctt ataaagatac tgttgaagca actactgcag tcaaaactgc
    10081 cgacctctat cgcctacata ttcttcctgt ttttggagaa aagaaaattt caataatcag
    10141 tcctttagac tgtcaaacct tcatcactga caaagccaag acattcaaaa acatgaagca
    10201 aatcaaatct tatacttcta aaatatttga atttgccatt aatatgaatt atattgaacg
    10261 aaatcccatg agcaaggtta ttatgcctaa actcaaaaaa actgtcagtg agaactattg
    10321 gacagttaac gagttgcatc attttttgac aatcatttta gaaaatgaac cttataaaca
    10381 ttatgcacta tttcgattac tagcatacag cgggttacga aaaggagagc tctacgccct
    10441 taaatgggaa gattttgatt ctgagaatca actattaaca gttagcaaaa gtttaggaag
    10501 aatagatggc catgccattg aaaaaggaac taaaaataca ttctctgtgc gctctatcta
    10561 tcttgatgat gaaacctgtt ctatcctcaa caaatggaaa caggaaacta gcagagaaaa
    10621 gtggcaatta tcagtacaac ctctttctct cgacaaagaa tttatgttta catactgcaa
    10681 ccgagatggt gagatagaac ccttacacgc tgactatatc aacaatattc taaaacgtat
    10741 tatacgtaag cataatctta aaaagattag cccacatggg tttagacaca ctcacgcaac
    10801 cttgatgatt gaaatgggaa ttgacccagt caatactgct aaacgcttag gccatgcaag
    10861 cagccagata actttagaca cttatagcca tgctacaaaa gctggtgaaa aacagtcaat
    10921 tacaaaattt gcagagtatc ttaataaggc taaatgaact agatttattg ctcagcctca
    10981 tattgaccaa aaaccaagtc tcgaaagatt accaaaagga ttaccaaagc acttttttca
    11041 tcaaaaaagc accttgcgaa aacttcgcaa agtgctctga aacgttgata taatcgtatt
    11101 gattaacgtt ttgagaattg tgatgccttg cgggcttttt tgagacctgg ttttttacgt
    11161 tcaaccatac gtgcgtcacg tgtaagaagt ccagcacgtt tcaatgaatc gcggaaatct
    11221 gggtcaactt ctaaaagtgc acgtgagata ccatgacgga tcgcacctga ttgacctgca
    11281 tatccaccac ctacaacgtt aacaaaaaca tcgtatgaac cttgagttga agtaactgcg
    11341 aaaggttggt tgataacaag acgaaggtct gcgtgtggga tgtactcttc tacatctttt
    11401 ttattgattg taattttacc agtacctgga accaaacgaa cgcgtgcaac agcgttttta
    11461 cggcgaccag tacctgcata ttgtgcttgt gccattatat aatgctcctt tcctcttaga
    11521 taagtcctga gatatcaagt acttctggtt gttgtgcagc atgtgtatgc tcaccgccaa
    11581 caaatacttt caatttcata ccttgtgcac gtccaagagt gttatgtgga agcatgcctt
    11641 taactgattt ttcaatcaaa cgaacagcat ttttagaacg aagttcacct gctgagattt
    11701 gtttcaaacc acctggatac attgagtgag tgtagtagat tttatctgat gcttttttac
    11761 cagttaattt aactttttca gcattgataa caatcacaaa gtcacctgta tcagtgtgtg
    11821 gtgtgaaagt tggtttgttt tttccgcgaa gtacgctagc aactactgca gaaagacgtc
    11881 caagaggtac atctgttgcg tcaacaacgt accatttgcg ttcaacttgg cctggtttag
    11941 ccatgaaagt tgttttgttc atgatttctc ctatacgaat tcgtttttgt ttacaggtgt
    12001 ggatgttccg gtccacgaga gtatttgaaa ggttccgggg ccttacaaat ggggtaaaca
    12061 ataccgctta taatcatatc aaaataaggt agcattcgtc aagttatttc ttttatttta
    12121 tcaattttca tcccaaaata aatcatcagt aatttctcaa agtaagttcc gtctgccctc
    12181 caaaaccgga agttagtgtg aaaaggattt ttatggtgga attaagtctg agacgtttgg
    12241 gttagaaatg agtttatcca tcatgacaaa acacaaacac ctaactctct cagaccgtaa
    12301 tgacatccaa tctggtttgg atagaggaga aacctttaaa gccatcgggc ttaatctact
    12361 gaaacaccct actactatcg caaaagaagt caaacgaaat aagcaactaa gagaatcaac
    12421 caaagactgc ctagactgtc cgctactaag aaaagctccc tatgtttgta atgggtgtcc
    12481 aaagaggagg atcaactgtg gttacaagaa gaccttctat cttgctaagc aagctcaaag
    12541 aaactatgaa aaacttttag ttgaatctag agaaggaatc cctttgaaca aagagacctt
    12601 ctggaaaata gatagagtgc tttctaatgg ggtcaagaag ggccaacgta tctatcatat
    12661 cctcaaaacc aacgatctag aagtgagttc ttcaaccgtt tatcgacaca tcaagaaagg
    12721 ctacctatcc atcacaccaa tcgacctacc cagagctgtg aagttcaaaa aaaggcgaaa
    12781 gagcacactc cctcctattc caaaagcgat taaagaaggg cgacggtacg aggattttat
    12841 agaacacatg aaccaatcag agctgaactc ctggcttgaa atggacacgg ttattggacg
    12901 gattggtgga aaggtccttc tcaccttcaa tgtcgccttc tgtaacttca tctttgccaa
    12961 actgatggat tctaagacag ctatcgaaac tgctaaacac atacaggtca ttaagaggac
    13021 actctatgat aacaagagag acttcttcga actctttcct gttatcctaa cggataatgg
    13081 tggggaattc gctagagtgg atgatattga gatagatgtt tgtggacagt ctcaactctt
    13141 cttttgtgac cctaatcggt ctgaccagaa agcaagaatc gagaagaatc ataccctcgt
    13201 gagagacata ctgcctaagg ggacttcctt cgacaacttg actcaagagg acattaatct
    13261 ggcactatct catatcaaca gcgttaagag acaggctcta aatggtaaaa cggcttatga
    13321 gctattctct tttacttacg gaaaagacat cgcaagtata ctcggtattg aggaaattac
    13381 tgcggaagac gtctgccaat ctcccaaact attaaaagac aaaatctaac ctaatcaact
    13441 aactgataca acctaaacgt ctcacactaa cttcaaccat agcggaactt aatctgaaac
    13501 gctttcagat gaggatgttt tcttgtacct ttttttgcga tttttaactc tgaaaaccta
    13561 tgaaatcaag tattataaaa accaacggaa cttagtctga gacggatttt cgttggtttt
    13621 tattggtttt tattggtttt tatcagacta aattccattt ctactagagg tggaacttac
    13681 tttgagaatt tagcaaaata aatcatctac agttttattc aaagcttgac acaatttaat
    13741 cgcttaatta tagcctcctt tttcaatggc ggctattgtc tgacatgata caccgacctt
    13801 ttcagctaga gactgttgta ataaatccat actagctcta gctgtcttta agcgtaaatt
    13861 tttcatatta ctatctgtcc ttatacttat ggtagtacac tgaagttccc atagctatca
    13921 atgatattcc tataactgct aaaattgcat catctcccct tgaaaaataa gtagcaatat
    13981 caaccttcct agtaaaagca tcaataaaat agttattcct atgagggcaa atgcgccagc
    14041 aacaaaaaca ttttccaaaa tctttcatca acaaacggag agactctttt tagattagaa
    14101 gtcgtaacaa aatagaaaca ccactaaata aagttaatat tattataaaa tcagatgata
    14161 cgctcccctt tattagtttt ccaaaaacac tttcaagcca taagataaaa accatcgtct
    14221 aaaaacattt ttgataactt tgtaaaacca attgttgttg tcgttcatca tattgaggat
    14281 agttcttatt ttatttttca taataggtct cctactattt tatagtttca tagcatcata
    14341 taaaatacat tttatgaaat atatactaaa agaacataac gtactgttat gccctttgat
    14401 tggttatcgg ttttcttccc ctaaagagaa cagagctaaa gcacctgagc ccacatgagc
    14461 tgaaatgact ggccctaatg gacgaataat aacttcttca atacgctcat ctgctaataa
    14521 ctgttctttc ataacttggg ctgaatcttt ctctccacta taagcaataa ttaaagtgct
    14581 ataagatagt gtttttgttg cctgagtcac aatctcttta ataccttttt tacggcccct
    14641 tgtcttggca aatggtacta atttaccctc gctatctaac ttaagcaatg gtttaatctt
    14701 agcaacactt ccaataatag ctgcaccttt tgataagcga ccactacgca ttaaatggtt
    14761 gagatcgtca actaaaaagt atgtgtttaa tttagggaga agtgattcaa ttttttgttt
    14821 cacttcttct aaagatttac cttcttgtcg ctctttagtt gctaacattg ctaagacacc
    14881 ctcaccacat gaagctgcca ttgtatccac aatctcaatt tgagcatcag gatatttgtc
    14941 taagaccatc tcacgcgcaa tagttgcact ctggtaagtc cctgataaat gtgaagatag
    15001 cgctaaatat aacaaggcat ggtcattttc tgcataggtt gaaaaaactt cttcaaactg
    15061 accaacgtta atttggcttg ttgtaggctt tgctccctct tgcatacgct ccaacaagaa
    15121 gtcactagta attttttcgt ctccgactgt ctcataagtc tttccatcaa gttcaatggt
    15181 caaacctata atatcgacat tgtgctcttg agcccacttt tcatctaaat ccgatgtcga
    15241 atccgttagt attttaaaag tcattgttac ctcaatctat tatttttatt tgataatcaa
    15301 agtagttcca atattatatc aaaaaaattg ttggctggct aataaagtgt taggttttac
    15361 ataccatcta aaaaatcctt gagttttcct agttgttcat cattccacgg ccttaacttt
    15421 ggcgtactca aatcgatttg actggacatc ttatccaaat ctgattttac tgtcgctaca
    15481 cgctgttcca attctctagc tgagacacgt aaagcaccct gagaaaatat agtccattgt
    15541 tcatttaaat cactggtagc aacggagact aaatttaaca cagattgatt aagttctgcc
    15601 gctgctcgtt caatatagct atccgcagtt tcgtcctcct cagtaaagat aacggatatt
    15661 ttgtattggt cataacgctg acgtacacca ggaacatact gggcatcaaa tacacaaata
    15721 atatcaatgt gttcgaaatg tgcgtaatga tttagctttc taagtaatac ttcccttgcc
    15781 tcttctaaac gattgctttt aaaaagctgt cgagtatctt tccaaaaagc aatcatattg
    15841 tagccatcaa cgagtaagat agaatgtttt ttcataggcg gtttctaaat acttcataca
    15901 tcaaaatagc ggctgcaaca ctggcattta aactctgaac atgaccattc atgggaattg
    15961 taatcatttc atctacttgc tttttgatat tatgagaaat accttttcct tcattaccaa
    16021 taactagggc taatttacct ttggtattcc acttatgcga tggtgtccca ttcatatcag
    16081 tgccaaatat ccagaagtcc ttatctttta aagtatccaa agtttgactg agattagtta
    16141 cccttgcaat aggaacatgt tcaacagctc ctgtagatgt ctttgaaacc actggggtca
    16201 ctccaacaga acgatgtttg gggatgataa tacctgttac attggttgca tcagctgtgc
    16261 gtagaataga acctaggttg tgagggtcgg tcagaccatc aaggattaaa atgagaggat
    16321 tttcttcatt ctctgccttt gtcattatct ctgataaatc cgcatagaca aattccgaaa
    16381 cttttagaac gaatccttgg tgaactccac catttgtcat gtctgataag atttttttag
    16441 gagtccagga aatggatact tttttttcag tcgctagtgc tttgacttta tcaacgttct
    16501 ttcctcttaa gtcatcttgc aagtataatt tattaccagt attagctctt aaactctctg
    16561 tcacagcatg taagccatag acaaggtcac ttgattcttc tttaaattgt ttatctttca
    16621 tagtcatatt atatcatgaa tgctatagca taaagctttt tatagcaata ttttaccaac
    16681 aaatcctaat gtctctataa aaaagagagg atatagcctc tctttttgtt tatttttcaa
    16741 tggtttcaat acaccactgt attagagttt ccaagcgctt tatctgacca gtcatatcca
    16801 agtaccccat aagagcctca aaacctgtag acatacgata ggtaataata tcagcatttt
    16861 tagcttttgt atgactattc gcattacgtc ctcgtttata aattaactgc tcttcatcag
    16921 ttaaaatatt ttcttctaac atggcattaa ttaacaatgc ttgggcatta gcagaaacat
    16981 attgcgttgc tttccgatgt aactgattag gtttggtaaa accctgcata attaaatggc
    17041 gtcggatata caaagaataa actgcatctc cttcaaaagc taaagcaata ccattaatca
    17101 aacgaacatc aatcacgagt ccacctcacg ccatccttag tatctaacaa tttaattcct
    17161 tgttttgcta attcatctct aatacgatca gcagttgcaa aatcacgatt agctcgtgct
    17221 tcttggcgtt gttctattaa agattcaata tcagcatcaa gcacttcctc ttggaataca
    17281 ataccaaaaa tttctaataa ttcagcaagg gtttccttaa cttttgaagt atagtgtcct
    17341 gagttaatcc attttgccat ttcaaagata acagtaatac cgtttgctgt attaaaatca
    17401 tcatccatcg cttcttgaaa tgctttcaca aattgctcta attcttcatc attagcattc
    17461 tcttggattg gtaaattgaa agtatttttt aaatatttaa ggttaacttc cgcatcatga
    17521 acggcctttt cagtgaaatt cacgggttta cgatactgtt gtgtcgctaa gaaaaaacgt
    17581 attacttgtc cgtcaacgct cttcaacata tcgtgaacag taataaagtt accaagagat
    17641 ttagacattt tctcgttatc aacgttaaca aaaccattat gcatccagta atttgcaaag
    17701 gtcttacctg ttttagcttc agattgtgca atctcattcg tgtgatgtgg aaattctaaa
    17761 tcagctcctc caccatggat atcaatagta tcaccaagga tctcagttgc cataacagaa
    17821 cattcaatat gccaccctgg gcgccctttc ccccaaggac tttcccatga gacttcccca
    17881 gatttagctg atttccaaag tgcaaaatct aaaggattct ctttaatctc tccttcccca
    17941 tcaacacgac cacttgcacc aatctctaaa tcttccaaag ttttattggc taattttgca
    18001 taatgatgtg atttacttac tctaaaataa acatctccat tagcttcata ggcaaattcc
    18061 ttatcaacta aaactttaac aaaatcaata atctcatcca tatagtcaat aactcgagga
    18121 ttttttgtag caggtttgac acctaatgca gcaacatctt ccataaacgc tgaaataaac
    18181 ttatctgaaa aggattttgt atccatacct gcttcagctg caccttttat aattttatca
    18241 tcaacatcag taaagttaga aatataattg acttggtaac cacaatactc aaaataacga
    18301 cgaatagtat caaaagctac tacactgcgg gcatttccga tatgtatata gttataaact
    18361 gttggcccac agacgtacat gttgacttta ccttcattga gaggtataaa atcttgtaaa
    18421 ctgcgagtca tagtgtcgta aatttttatc atggtttttc ctttttctgt ttttgataag
    18481 ctttatacaa acgaacaata tacattaaag caaaggcagc ccaaataatt cctaagagag
    18541 agttaccttg tgcaaacttt aaatagctta aacaagctcc tgctagtata cctaaaattg
    18601 ataatatttg agttgttaca taatccttca caaatacctc ctaaagtttg gacgaataat
    18661 aactttcttc tcgatcatgt tcaatacttc ggatttgaag atcatccttt tgaccatgga
    18721 cacggacaac tttagctgga acaccaacta ctgttacgtc tgctggcaca tctgcaagga
    18781 caactgctgc agcaccgact ttagcatttt ctccaacctc aattggaccg atgatttgag
    18841 aatgtgctga aattagcgca cctttacgta tggttgggtg tcgtttgcct ttgtctttac
    18901 ctgtgccacc caatgtaacc ccatggtata gcatagcacc tttctctaca atggcagttt
    18961 ctccaatgac caatcctgaa ccgtggtcaa taaaaacccc ttccgaaatc gtcgctccgg
    19021 gatgaatttc aatttgtgtc cagaaacgcc aaaattgact gtgcatacgt gctaacaatt
    19081 taaaattatg attccataaa aaatgagata gcctatgagc tgcaagagct tttattcctg
    19141 gataagttaa aataacttct agagaactac gagcagctgg atcttgttct ttgacaatag
    19201 ctatactttc tttccaccac cccatagttt tcctcaatcg ttttctacat cagaccaatc
    19261 aactaaaact tgttgttcat caaatggatt gatggctact ccaaaggtat catcaatctc
    19321 attttctcca ttacgcggtt tataaatatc gtcaatcgtc caggctaaaa ttactccttg
    19381 tgctttccta aaagcaccac caaaatcatt atggttatac catttttcaa aacttaaaag
    19441 gtttgagaat actggaacat aactttttcc ttcgggggta gacattgtag ggaaaaaacg
    19501 gtcaaaacta ccttcttcac gtggatggac aaaggcaggt accaagtagg ttttatccat
    19561 gatatcggca acaatgttat cttcatttaa caactggttt aagattgtgg tgtaattgtt
    19621 cataaattgc accatatctt cacagtagaa aatagtcgag ttcccaaaat cgccttcttt
    19681 ttttaaatta tagaccaatc ctgctaagcc atgtgaaata gcttcatcaa gaacgtctaa
    19741 actacgacga ctctcccaaa acatatctcg agcactttct tgctcctctt tgaaatgatt
    19801 aaggtcagta atatccgtaa aaacaggaac aacttcttga ccatctactt gaatagcata
    19861 gggttccttg cttgcccaaa ccgtagagtg gtgcaaggca ttcacaagac ctatactatc
    19921 taaaaaatta tccggtgcat tgataaaagc acgcaatcta atgtctaact cgtttgtact
    19981 tgtcatatta agattccttt ttaggattgt cagcttttgg aggacgtggt aataatgctt
    20041 tcatagaggc atcaactcgc cctttatcat caattttgat gactttcaca tcaacttctt
    20101 caccaatttc aagaacatca gctacatttg ctgtacgtgt ccatgcaatt tctgaaatat
    20161 gtaccaatgc atctgtttta tcaaagaggt tgacaaaggc accgaatttc tcgatacgaa
    20221 ctactttagc atggtaaact tctcccactt ttgcttcacg tactaaactt gcaataatgt
    20281 ctttcgtacg atcaatagca gcctgatcac tagagaagat agatacattt ccttcttcat
    20341 caatatcaat cttaacacct gtttcagcga tgattttatc gatagtttcg ccgcctttac
    20401 cgatgacaac tttaatctta tcaacatcga tttttatcat atcaatctta ggtgccgttg
    20461 gtgctaattg tggacgtggc tctgcgatag ctccatgaag gacatcaaga atttcaaagc
    20521 gtgctttttt agcttgcgca agagcttctt ccaaaatttg aggggtaata ccttcaattt
    20581 taatatccat ttgaagtgct gtaatccctt cacgtgtacc cgcaacctta aagtccatat
    20641 cgccaaaatg gtcttctaat ccttgaatat cagtaaggac tgtgtagttt gttccatctg
    20701 atatgagtcc catggcaata ccagcaaccg gagcttttat aggcacacca cctgccataa
    20761 gagcaagcgt tccagcacat atagaagcct gagatgatga accgtttgat tctaaaactt
    20821 ctgcaactag tcggatagca tacggaaact cctccaagct tggaaggact tgttctaatg
    20881 cacgctctcc taaagcaccg tgaccaattt cacgacgccc tgcagctcca taacgtcccg
    20941 tctcaccaac tgaatattgt gggaagttat agtggtgcat aaagcgtttc ttatactctg
    21001 gtgttagccc atcaatgatt tgtgcttcac ccataggtgc taacgttaat actgataatg
    21061 cttgggtttg tcctcgtgta aagagacctg atccatgaac ttgtggtaaa aaatcaatca
    21121 ctgcatctaa tggacgaatc tcatccacac gacgtccatc aggacgaacc ttatcttctg
    21181 taatgaggcg acgaacttct gcatgttcca tttgttctaa aatttcagca acatcacgca
    21241 taatgcgatc atattcttca tgttcagcgt aacgcgcctc atattcacct ataactactt
    21301 ctttaactgc ttctgtagct gcttctctag cctttttctc ttcaacttgt actgcagcct
    21361 gtaaagctat gttatgagta gcaataattt ctgcttgaag ttctggatca acttgaagaa
    21421 gttctacttc ggctttttct ttaccaatag cagttacaat ctcttcttga aaggcgatta
    21481 attcacagac agcctcatgt cctttaagaa gagcctctag catgatttct tctgaaagtt
    21541 ctttcgctcc agattcaacc atgttaatcg cttctttagt acctgcaacc gttaattcta
    21601 acgcagaagc ctcttgttct tgtgctgtcg gattaataat aaaattgcca tcaacataag
    21661 caacttgaac tccagcaatt ggcccattga aaggaatatc ggaaatcgat aatgctaatg
    21721 atgaaccaaa catagcagcc attggtgcgc tagcattttc atcaaatgaa agaacagtat
    21781 tgatgacctg gacttcgtta cgaaatccct cagcaaacat cggacggatt ggtctatcaa
    21841 taagacgtgc tgttaaggta gcatccgtac ttggacgccc ttcacgtttg ttaaaaccac
    21901 ccggaaattt cccagcagca tacatttttt cttcgtaatt gacttgaagt gggaaaaaat
    21961 cacctgttga cattttctta gacataactg ctgctgtaag tacagtagaa tctccataac
    22021 gaacgacaac tgatccattt gcttgtttag ccacttggcc agtttcaaca actaattttt
    22081 taccagcaaa aatcatttca aagacttgtt ttgacatatt attctccata tttttttgtt
    22141 tacgcgtttc ctacaaagtt caatctagct gttctaaact caactttgca taaaaccacg
    22201 tataagcttt attctaacat aattctataa aattgagtag cgaaaatccg tgggggaagc
    22261 tgtattatca ttcattaatg taaacgtaaa aagtctgatt attaatacta gcaatcagac
    22321 cttgttacaa tgattaaaat aaatttctag ggttaatctt agcttgctta ataaccctct
    22381 tcataggtta aaacactttc ttactgttgg ccccctcaaa gtacttacgt tcatagtacg
    22441 tttaaaacaa aaaacactag aaaattttct agcgttttta cttgactctg ttgtctatta
    22501 acgacgaagt ccaagtgatt gaatcaattc acggtaacgg ttaacatctg tacgacgtag
    22561 gtatgctaat aagttacgac ggtgaccaat ttttttcatc aatccacggt aagtagcgtg
    22621 gtcttttttg tgttgtttga tgtggtcatt aaggtggttg atttcccaag taagaactgc
    22681 tacttgaact tcaactgatc ctgtatcacc ttcgtgacgt gcatattgag caatgatttc
    22741 attttttttc tcttttgaga ttgccataat atattctcct tttattgctt catccgagtc
    22801 ttaggattgg cactcctaaa accaagaaaa agttggtttg tcttatagac cctcttattt
    22861 tatcaaatta gaataaactt tgtcaaaagt ttttaataga aatatggata accaactgtc
    22921 tctaaaggaa aatccttcat agactaaacg agactttgca tctttttgcc gaaagacctt
    22981 agcataagta gatgtcatac tagttggccc acacataaat atcgttggtt gtccttcaaa
    23041 aacagactga ctaaaatcaa gtcttccttg gaggctacta ttatttaagt gaagtttaaa
    23101 attaggattg gctttagcat aggattctaa catatcttga taaatcaaat tgtcttgatt
    23161 tgaaaatgta taaaagaagt caactctctt agtcaaaata ctattttctc taataaatga
    23221 aatgaaggga gtgatgccga tacctcctgc aatccaaacc tgttctttct tgtctttatc
    23281 aaataacata tgaccataag ctctatctaa ggctattttt gtcccaactt ttagctgttt
    23341 gtaaatggat ttcgtataat ctcctgaagc tttaacagtt aaaaatatca ctctatcatg
    23401 accacccgat atcgagaaag gatgagctgc tgattcaaag ccggcttgat aaatttttaa
    23461 aaaagtaaat tgtccatagt catatcgata aggacgcttc attgctattt caatctcagt
    23521 tgtatcatgg ttaagatgag taaccttttg aacgtaccct acacgacgaa aacgcattct
    23581 agaatatagg aaaataatat aaaatccaga tataacccca atgacagcat aacctaatac
    23641 gattaaactg agtaaagtat tccctagtat cctatctcct aaaatcagaa aagtatgtac
    23701 caatcccaaa atatagacta aataaacaaa gcgatgaata aaacgccaaa tttcatactt
    23761 taaaaaattt ccgaaataag ctacaaaaac aatacttaga aaaagatata atcccgcact
    23821 gccaatagtc tttgcaaact cacttccatg tccgccttgt ccaagaccaa ttttgtgcaa
    23881 tagaagcaaa atcattgaaa atacagccat taccttgtgg taaaaataca tattttcaat
    23941 accttgaaag taactttcaa gtatcctaaa tttggttgat aagaggaatg ttagcgataa
    24001 acttgtcaaa gctaatcctg gtacaacaaa ttcacttaat cctgcatttt gccatgctac
    24061 tagtgtcacc actatgctag ataaaattaa aacaatacct ttccaagctc gcataacgac
    24121 caccccttta tttttagtaa ggataaaata ccacaagata cttatacaaa acttaaaaat
    24181 ttcagaaaat aacaaaaagg caactccaat gttgcctttt tgttatgatt tataaattga
    24241 tttgcaatgc tctactatat ctttaacagc gagtccatac tcctctaata aatatttcat
    24301 ctgacctacc tgaccaaatc gctcatcaat acccatacga ctaacagtag tgtttttctc
    24361 catactaagc cattcgcata atgcacttcc gataccgcca atacggttat gattctcaat
    24421 agttacaatg gattgatcta tcagtaatgg tttcagttct tctggaaggg gcttaatttt
    24481 aaaaaggtcg ataactgagg cctcaatccc tagttctttg aggtagtcgg ctgcttctat
    24541 cgctctagaa accataatac cacttgctac aagagcaata tcttttccct ggcgtaattg
    24601 tataaaacct tttgaaaaat cttcgcatcc ctcataaact gcagtggggg ccttacgaat
    24661 agttctaatg tattttagac catcaatact taatgtttgt tttaaaattg cttcaaactg
    24721 aatatcatca ctaacctcaa aaatagtcgc ctttggaatt aaccttagta gacctaactc
    24781 ttcaaaaggc atatgagttc cgccattcat ttctgcgcta actcctgcat ctgagccaat
    24841 aatcgtagct gataattgtg agtaacctaa cgataaaaat acttgatcaa agacacgcct
    24901 tgacgcgaaa ggaccaaaag tatgaaggta tggcttgtaa cctttaatgg cgagccctgc
    24961 tgcaagacct accatctcag cctccataat acctagatta atataacgtt ttccaaattc
    25021 tgatgccagt acatttgttg acattgaact cgacaaatcg gcttctaaaa cggtaatttg
    25081 tttattttct tgattagctt gaaggaggaa atcacgatag acaagcctca tttctttggt
    25141 tgaacatctc attctaccac ctccaaactt tctctcaagc ttatcaccgc tcgctctaac
    25201 attgtttttt gttgtaaatc tggtcgtaaa tgatgattag aagctaactc ctccaactct
    25261 ttcacacctt gtcccttgat actatctaaa acaatgcatt ttggtttgac actatttgaa
    25321 tcttgaaaag ttttaatagc tttgtcaatt gcctcaatgt catctccttt tacacgtact
    25381 gcatcaaatc caaaagcttc aaatttagca acaaagtctc caggattaca aatatcagct
    25441 gtcaaaccat ctaattgttt tttattatca tctacaaaaa caattaaatg gtgcagttga
    25501 tgatgcgcag caaattgtat agcctcccaa cattgtcctt catttaattc accatcacct
    25561 acaatagtat aagtataata gcttgaattc tcaatctttt gagcataagc aattcccgtt
    25621 gcaatactaa tgccttgacc taatgagcct gtcgttacat ctataccagg agttaaattg
    25681 cggtcaggat gcgaaggtag tttggtaccg tttgtattga gcgaatgaag gaatgtttta
    25741 tcaaaaaaac cttttaaata gagtgtacta tacaaggctg gaccagcatg tccttttgat
    25801 aagaccatat aattccgatc actttctttg aatttttcag ggtttatatc cataatatca
    25861 ccgtacagaa tagccaaagt ctcaacaatt gataaactac ccccatagtg accgaatcct
    25921 aaatggttta gcgtttctag tgtatttaaa cgaatctccg ttgcaaatcg tcttagagat
    25981 gaatatcttt cttctggtga tgtcattatt aaccttcctt atgggttgat tttccaacaa
    26041 atgaaatgcc aaaaagtcca attaagataa ctacaatacc aataacaata gctatcgcac
    26101 cgccgacatg attaagagcg ccaagaatga taccggtcaa accaaaatca gcatctgaga
    26161 atgttgaacc tttgaaacca agtccaccta gtacaggcat caagaaaatc ggcaagaaac
    26221 tgatgaggat accttgaaca aatgcaccaa tagtcgctcc tctcacacca ccagaagcat
    26281 taccgataac acctgccgtt gccccacaga agaaatgagg aacgacccct ggtaaaatga
    26341 cagtagtgcc agtcacaatc ataactatca tactaaccaa tccaccaaca aaacttgaga
    26401 taaaaccaat caatactgca ttaggtgcat aagggtaaac aattggacaa tccaaggctg
    26461 gttttgaatt tggaaccaat ttttctgaga ttcccttaaa ggctggtaca atttcaccta
    26521 ggataaggcg tacacctgct aaaataacga atacacctgc tgcaaattgc ccagctagtt
    26581 ggagggcata aacaagacca ttgacaccgt tacttatttc ttttgcaata taagcctcac
    26641 cagcaaacac tgcaacgata aggtaaataa ttgccattga gatagtaata cttacggtac
    26701 tgtcacgtaa aaatgataaa ctctttggaa atttaatatc ttctgttgat tttgatttat
    26761 ctccaacaat tccaccaata aatccactca accaatagcc taaagaacca aagtgcccaa
    26821 gtgccacctt atcattacct gtcagttgaa tcatgtattt ttgcacaaat gcaggagata
    26881 cgctcataat aatccctaac gctagacctc caaaaaggat gagcgagaac gaagtaaagc
    26941 ctgctacggc aaaaataaca gcaatcatac aagccatata caatgtatga tgtcctgtca
    27001 gaaaaatata tttaaatttt gtaaaacgag caattaaaat attaaaaatc attcccgcta
    27061 acataatcaa agcagttgct gagccatact tcgtaagagc tactgctaca atggcttcat
    27121 tattaggaac tacccccacc aaatgaaaag catgctcaaa catttttcca aatggattca
    27181 aggaattttg gactatccct gcacctccag aaaccactaa gaagccaaca aatgttttta
    27241 ttccaccttt tacaatatca ggaacacctt ttttctgcag tactaaaccg ataatggcta
    27301 tcaaggcgac taaaatagct ggcgtgctgg caatattaac taaaaaatcc aatagacctt
    27361 tcatggcatc aacctcctag attaagcctt tttcttggca aatacctgtc actaattctc
    27421 tcaattcatc catatcaatg atactgttta aaatacgaac atcccccagg tgaccagctg
    27481 aatcctccaa atcacgacca acaatccaaa catctgctgc gcttggatct gctccaccta
    27541 aatcataatg ttcaacttca acatcagaaa cccccaaatc cttcaaaata gactcaatat
    27601 tcatttgtac cataaaactt gaacctagac ctgatccaca tgctgttcca attcttaaca
    27661 tctttaatcc tcctgtttta taatcatttc aatatcttga tacccacgtg aagctaacaa
    27721 agctttaaca ttattattat cacgaagtat tgttgtcaaa tgcgatagag ctttcaaatg
    27781 cgtttcatta tcaatagctg ctatgcataa taagagataa atttcctgtt tggggtcatc
    27841 caataaataa ataggctgtt ccaaaacaag cattgacata ccgacagagt ttaccccatc
    27901 ttctggtctg gcatgtggaa tagcaatacc tttaccaaga ttgataaaag gaccaaactc
    27961 ttcgaccttt tcaatcatag cttctgggta actttccgta atttttccag aagctaacaa
    28021 gggttttgcc gctaaacgaa ttgcctcttt ccaattcatt tgttcagaac ttgtctgata
    28081 agtttcctct gttataagtt ggtgcaacaa gggcctcaca tcctttctaa atatatcttg
    28141 ttttaccatt gtcctcaaag ccaattttaa ttcctcttca caatgaacat gagcgtattt
    28201 ttttatagtt gctattaatt gatcaagttg gaaatcgtca aggcaagctt tgggaaaatc
    28261 tgaaataacc agttctttga gctgttgtac ttgctctgct gtcatcatca gcgaaactag
    28321 ataattaggt ttttttgttt ctacaaatat agtagaaaag accatatcgt aagtttgatt
    28381 gtcaatcagt ttcagctgct caattttaga gacacgatga aaatgaattt gaggaaataa
    28441 tccacgcaat ttttccttaa tcactaagga tgaactaacg ccgtttggac agagtatcaa
    28501 agctttataa gacattgatt gtgtatcacc tgattgtcta agatagcctc caaagtgaat
    28561 cacgaagtaa gaaatttcac tatcgggtat taatcctact tgctcttcca aaggacgtag
    28621 tgccttttta accaatagaa ataaatcact gtaatgctct ttaatatttt gtgtataacc
    28681 actatcgcca gttaaaccat atttcaatcg gaaataagct ggaataatat gacgcttcag
    28741 accttgtaat aactcttctt tattggaaaa attaagaagt gaaacagttt ccatctcatc
    28801 aacgattgct tttgccaatg cttcaaagaa atcatcatct ttagttccct caccttcaaa
    28861 acagccagat aagatcaagg taatataatc tttttctttc tgatctaatg gtttagataa
    28921 ggaaatatcc tgacttaaat tagtcacaat aatagtcgtt aactctttta attgttcaga
    28981 aacaatagga gaaccctgca aaacacgatc tactgacctt tgataacgac acagtattaa
    29041 aatgataaaa tataaagatt tctcaagcct atcttgtatt ggagaaagtt gaaaagattc
    29101 atagaaatat tctaccgtct tttctaactt ctcatatgag agagcgaatt tccaagaaga
    29161 caagatataa ttcaaagacc aaaaaccaat gggagattct agtagttgag ttatcatctg
    29221 aaaagcaact tgatgcttat ccatctcatc tcctactaga ctatatccct ttgctcttgt
    29281 ataagtgagt gagatacctc tttttgctaa tttagaacgt agcatcttga tatctgataa
    29341 agtagtattc ttacttactt tcaaaaaatc ttgataatgc acattggaga caaactctct
    29401 tcggcaaaag ctataaaggt aaatcagaca taccctttct tcctgagtta ggtaaaattg
    29461 ctcagcttct aataaggaga caattttatc atgctctttt tcatctccta ataactgata
    29521 acgtccagca gagtgagcaa tagctggtag gtgatttttt gctaaaaaac tattgagata
    29581 gttaatttgc tctatgataa cttttattga aaactttgtt tgcactgtta actcatacaa
    29641 ggaaagattt ttccgagcta aaaataccaa cattatcaca acagtcttat tatcaaccat
    29701 ttgttttcct ttctattttt attttatagt aaccgtttac atttttctag gtcatttgag
    29761 cccaatctgg cactactatc aattgaatga atttgggcta ttgttataga gtaaagaaag
    29821 aaaaaagaaa gaaattgcaa aatctagagc aagattcctt cccacaagca gcacttaaaa
    29881 aacctttact taattgaata agtaaaggtt ttttaaatgt tatgacacta agatagctaa
    29941 agtttgataa ggttgcaaag taaccgtttc atcaagagta acatccttat aatttgatag
    30001 aagaacttgt ccttggagat aattctccgg taattttatc ttaactttct cagcaaagaa
    30061 attatttaaa acaagaagtt tttgcttatc aagatggcgt tcaaaagcat atactttttc
    30121 gttatctttg aaagctgctt tataattccc gtcagctatt attggcagtt gtttacgtag
    30181 ccttatcaac tcttgataga aagtaaatat cagccctgtt ttctcctttg cgacattgat
    30241 ttctttatat gattttccta ctttcaacca tggagcgcct tcagaaaaac ctgcattcgt
    30301 actatcatcc cattgcattg gtactcgaga attgtctcga gatttcgctc gaataatgct
    30361 aaatgcctct tcttgtgact tgccttcatc aagcattatt tggtaggcat tcaaactctc
    30421 aatatccaca taatcatcca tagaactgta atcgggatca agcattccga tttcttcacc
    30481 catatatata taaggagtac cacgcgataa gtggatagat gctgctaaca tggttgctcc
    30541 ttcatttcga aaacgtttca cgtctacaaa acgatttaat gctcgcggtt ggtcatgatt
    30601 attatagaat aaagcattcc aaccatttcc ttcgctcatt ccttcgcccc aactatggaa
    30661 aaggtcacgt aatgcaggaa aatcaaaagc cataatggtc cacttttgcc catctttata
    30721 atctactttg aggtggtgaa aattaaaagc catggataac tcttcacgtt ctggtgcagt
    30781 gtaaagaatg caatttgcaa ttgtcgttga agacatttca ccaactgtca tgaaggaatc
    30841 agcttgtcca aaactagcat tattcaacat cttgagataa tcatgtgtta ttggacgatc
    30901 agtataagct ggttttccat cattaattgg gcagttctca agtatctcat ctttcccaat
    30961 taaattaatc acatcaaatc gaaaaccttt aaccccttta tcacgccaaa aattgacaac
    31021 tttaaaaagt tccttgcgaa catcggcatt gcgccaatta aggtcagctt gagtgatatc
    31081 aaaaagatga agataatact tccctgtatc accaaatggt gaccaagcat ttccaccaaa
    31141 tttagatacc caatctgttg gattatcacg taagataaag aaatcttgat agtagcgatc
    31201 tcctgcaagt gcttttttaa accactcatg ttctattgaa caatgattaa gcaccatatc
    31261 aagcataaaa tctatacggt attgacgtcc cacctcaatc atttcttcaa aatcgtccat
    31321 ctttccaaaa tcagggttaa tggctgtata atcagagata tcatatccat tatctcgttg
    31381 tggactcgga taaaatgggt tgagccaaac catatcaata ccaagttctg ctaaataggg
    31441 taatttttcg ataataccac gaagatcacc aaccccatta ccagttgtgt ctttgtatga
    31501 ttttggataa atttgataga caactttacg tttatcaatc gtcatattct actcctttcc
    31561 caaaatgctg ctgtatttag cagtctaact ataacatcaa ctatcatcaa aaatataaca
    31621 tttttacagc gatatttgtt cccatgagtt ttttcttatg ttcaaaataa ttcaaggagc
    31681 taatgtatta agcccctcaa acaattcctc aaattacctc tcattttata agagtgctct
    31741 tttttaaaaa ggatacctaa ttacttttaa attttcttgg caaccattaa tgcttgtcca
    31801 cgttttatct gtttgggtaa atctatcaat tcttctggtc ggaattcttc ttgattagta
    31861 atcagaatag gagtttccac aatgtatcct tcttctttta tcattggaat atcgaaagta
    31921 atgagtttat ctcctacttt tacattgtca ccttgggcaa catgagaggt aaaccctttt
    31981 ccttctaaat taacagtatc cataccaata tgaataagaa actcaacacc ctctgatgtt
    32041 aataatccaa tagcatgttt cgtagggaaa agaactgaaa ctgtcgcatc gaccggtgaa
    32101 actagttctc catcacttgg atcgataacc actcccttcc ccataattcc ttgtgaaaag
    32161 acaggatcac tagcttgatc cagtggttta gctaaacctg ataaagggct tactacacta
    32221 agctttgtac ctgagacgac agctttttct tttgctgatt ttgtttctgt tgttgaagca
    32281 atgacagcat cagatactaa tttttcctct tcagtttttg tcaaaatacc tgatttttta
    32341 aagaatagtg tcaaaaataa aggaataaag atagccactg ccatacagat aaagaaatat
    32401 cccatatatt taacattaat tgacaggaag cctggaagac cgcctacacc aatagagtta
    32461 gcttggacat taaaagttgt cgccaaaaga cctgccacac ttgaaccaat cataccagca
    32521 acaaatggat aaatgtattt aacgtttaca ccaaaaagcg ccggctctgt aacaccaagg
    32581 taggctgaga tagcagctgg aagtgatatt tgagcctctt tttcatcatg acgatgcata
    32641 aaataataag ctagtacagc tgaaccctga gcaatatttg aaagagcaat catcggccaa
    32701 aggccagttg tatgtgtttt tgtatctgct atgagttgag tatcgatagc atttgtcatg
    32761 tggtgcaaac cagcaataac aaatggggca tacagggcac caaaaatagc accaaagagc
    32821 cattttacag gaccagtcaa tccaataaga acgatagcag aaatccattt acctagtgtc
    32881 caaccaatag gtccaaggac agtatgagcc aagataatag ctggaactaa ggatagaaat
    32941 ggcacaaaaa tcattgaaac aacttcagga atatgttttc tccagaagat ttctaagtaa
    33001 gacaggctca aaccagctaa cagagctgga ataacttgag cttgataacc aattttttgt
    33061 actgtaaagt aaccaaaatt ccaagaccaa tttttagcaa tgtctgctgc tgaagtacta
    33121 gctacagaat aagcattaag taattgtggc gacactagac agatacctag aacaataccg
    33181 aggatttgag tagtccccat cttacgagtc acagaccata caataccaac tggtaagaaa
    33241 tgaaatatag cttcacctgg taaccataaa aaagagtcta ctccgctcca aaacgttgaa
    33301 acatctacta gagtattcca aattggatgc ccagaagagt ctacttgcct gacaccatca
    33361 acaacttttt gacctaaaaa ttcaaatgga acggcatcta aaatattacg aaaaccaagg
    33421 atcagaccac caacaataat ggctggaata atcggtgtaa atatttcagc cagcattgtc
    33481 aacacacgct gtaaagggtt ttgatttttt tgggcagccg attttgccgc ctctttagac
    33541 acaccctcaa taccagaaac ggctacaaaa gcattataaa aaatagggac atcattacca
    33601 ataatgactt gaaattgccc agcattggta aaggtaccct tgactgatgg taactcctca
    33661 attaccttta ccttagcttt actactgtca ttcaaaacaa atctcatgcg tgtggcacaa
    33721 tgtgtcactg cactaatatt ttctttaccc ccaattgcct ctaacaaggc tttggcatca
    33781 tgtttaaatt gttccatctg tttatcctta tctatttaac ggagagtctg tgactccaag
    33841 gcagaaactt aaagaagact agcttacaat ataattgtaa acgattacat atccctatgc
    33901 aaatccttag cttatctttt taaaaaatct ataaattata aaataattag tcaataatac
    33961 ccaaaacatt agaatttctc accaacgtta ccaaaatgtt agagtcatga gtaggtcagt
    34021 cacccccaat actctcttaa taaaaaacac ctactaattg atatagtagg tgagtttttt
    34081 aatctccatt gaaagatacg atacgattct cttcaatcat atagcattgg ttacaaagtt
    34141 tctctaacat agcgaaatca tgactaataa cgatgagacc taaatttcta tcctttacaa
    34201 tctccaacag gcttttccat acactagctt gtgtaatact atccaacata gtagtcattt
    34261 catctgcaat aaggtattta gtctctggat gtaatgaacg cacaatcgaa aagcgttgta
    34321 attcccctcc cgagagttca ctagggcgac gatttagcca tttttcttga attccaaaag
    34381 catctagtaa atctcgactt gggtaataag cttcttctaa actttttttc ataggccata
    34441 aaggattcat cgttttttca ggatgttgtt gaataagttg tacaggtcta aatgctttgt
    34501 taggcaaatt actgccatca actaatactt caccagatct aggatgtaaa aaacctgcaa
    34561 gcactttgga taaactagtt tttccacaac cactttgtcc aaatatacct aaaacttgac
    34621 caggtgctac ctctaaatta atctccttaa aaagccattg atctttttta tgataaaagc
    34681 caagcttttt agcctctaag gtcatgagta actcctttca aaaattcttg ctgtgggaga
    34741 gagcgcaata aacttctagc aaattctgtt tgtaactgct ctccatttcc gctaaagaaa
    34801 ctagctggag ctgtttcaat agcttttccc tctttaaaaa tagtaatacg atcagcaatt
    34861 tgactagctg ctacaatatc atgagtgata aatataacgc ttattccttt atctgcaaag
    34921 gagcgtagtt ggtctaaaac catttgcaga gcatctggat gtaatccagg ggtgggctca
    34981 tctgcaataa tcaaagaaac cttatcacta atacacgttg taaacaaaac acgtcggagc
    35041 attccgccag aaagttggaa aggatacaag tcaccatcac tttcttttaa accaaactgt
    35101 tgaaacaatc cttcttgagt agcctttgaa ttttctgaga tacctaagcg cacctgatgt
    35161 ttgactttca tagatggatc taaataatta actgattgtg gaatcaacgt aatatctttt
    35221 cctcgcaact gtttaatgcg tttagaattt agtgattgac cacgataaat catatctcct
    35281 gttacagatg catttttagg aagaatatcc ataatagcat gtgctaataa actcttcccc
    35341 gaaccactag cacctataat agctaataac tcaccttttt taatttctaa attcagcgct
    35401 tgtatcggtg ttgattgaaa tggttttaaa aatcttccgt attgagtgaa ggtgatggag
    35461 aggtctttaa tgcttaataa tgtttctgtc atatctctac ctcctaaaaa tgatcagttt
    35521 gagggtaaaa gagtttcttt aaagattctc cgatagtatc aaatgcattg acaaccaaaa
    35581 taagataaag tcctggaaag ataaccaacc accaatttcc aagagagata tgcttagctg
    35641 cctctgacag aatgatacca accgaaggtt gttcggcaga gagcccaaat cctaagaaag
    35701 tcattgatgc ttcatgtagg atgacatgtg gaaataagag gataaaacca atgaaaattt
    35761 gagaagcaat caaaggcagg atatgatgcc tcacaatata ataaggcgtt tttcccatac
    35821 ttttagaaag ttggacaaat tctttattct ttagatgata gacttcattg cggataagcc
    35881 ttgctaaaga aggccaatgt gtaacagccg ttgcaatgat gaccccttga gcacctttcc
    35941 caacaacaaa agaaatgaga atcataaaaa tcaaatgagg cataccaata aacaaatcaa
    36001 ctaaccatgc tattatttta tcaataatgc tatttcctaa acctgcaagc actccaaaaa
    36061 ctgtcgccag aatgaccccc ataagggcac ctaataagcc gacttgtaga gagaaataaa
    36121 gtcctttaat cgttctgaca aacatatccc tacctaaacc atctgtccca aaaagatggt
    36181 ttaatgaagg agcaaggttc cgtaaagctg cattggtttg caagggagta ctatggaaat
    36241 aaagatttaa ggctagaata ctaagaatga gagaaatggc gatacccagt tgccataaaa
    36301 caatagttcg acgtctcaat atcatacttt tctccttaac tgtggattga ttatgctatt
    36361 taaaatatcc gcaataagat tgcccgcaaa aacaaataat gtccctatca tcacaatagc
    36421 tataagtagc ggtgtatcac ttttaagtcc tgcttcagtt agggtagacc ctagtcctgg
    36481 atatgagaaa acttgctcag caagaacgga tcctccaaac aattctccaa aataggaaaa
    36541 atgcagtgta atagctggta cgatagcatt tctaagacaa tgatttttaa aaatttgcca
    36601 ttccgtttcc ccacgcgctc tggcaaataa gacatattca ctagaaagta ccgacatcat
    36661 tttagttcta gtatgaagag ttacattggc aatgcctaga atacttagcg tgaaaacagg
    36721 taaaataagg tgcttaatac gatcagctaa cgtaatatct tgactcaaag tacctatcgg
    36781 agaagaaata ccaatcggga accaccccag ctggacagaa aagattaata aaaaaatgag
    36841 cccaatccaa aaagttggta ctgatatctg aaggtaagaa aaccacctta caattcgatc
    36901 aagcaatttc ccttgatgga aagctgataa agttcctaag ataaatccaa taagacccga
    36961 taagacccag gagagtccca taagtatgaa agaggcacct gcccgtgatc taataatatc
    37021 aataacaggt tgccgataaa ctagtgaagt ccctaaatgt ccctgtataa aattttttaa
    37081 ccaaataaaa tactggaccg gagctggctt atccaagcca tagtggtgag caatcgcttt
    37141 gtactgaaca ggggttagtg acgtgtcata attgacactt gccatgactg gatcaaccgg
    37201 tgattgtttt agtaaaataa aggtcaaaat tgatactcca aaaacgagtg acaaacacct
    37261 aagtatcttc cagataacta cagtggtaat atgtttcaca gataaatctc cttaattgtt
    37321 aaaaaaggag tcgaggaaac cattcacacc ataaggtgta cattgttttc tctgactcca
    37381 aacttaaagt agcctacatt aactaaatta gttacttagc agattcatcc caagtccatt
    37441 cagcaatgtt agtcaataat gaccaatcat gaccatgact atggacacct tgtttaccta
    37501 cattgatacg tttatcacca atataagtat ggttaagact caccaaccat acatttggta
    37561 aatctccaag agtggaagca cctgttttgc catcccactg agctaacttc caatatttgt
    37621 tagctttatc aaggtcggga gatgtcattg ctttgtcaag gtacttagtc acagtaggat
    37681 tgttataaaa agtaatattg gtccaacctt taccagctaa acttgggtaa tgtgattcat
    37741 aaaattgctg cgcatgatga cgtcctcccg cataaagtaa ggctgaatca tgtgacttag
    37801 ttgccatttc atcccagtta ctagccttga gtttaatagt aatacctaag gctttagctt
    37861 gctccgctac ttcaacggct aagtttgctc gtaattgatc attagtaggg tagtaaaggt
    37921 caaattccgc tttaagattt ccttttttgc ggctaccgtc tgcttgttct ttccatccag
    37981 cttttgtcaa aagttgctta gctttagcta ctttattatc tttaattgct gtttttggat
    38041 tccagaatgg tgttctatca ataattgaat aagctggttt accataacca tttaaaacag
    38101 tatccagaac tttttgtcta tttaaaccaa tagtcaaggc ttttctgatt gctggatcac
    38161 tagtgacatc atttcctact ggataaccgt ctggtgaatt tttcacaaca cctttcttca
    38221 cataaggtag cgataaccca cgaacatcat ttgaagcaat atctagaaga cgtgttcctt
    38281 tgactttctt gctagcaagc tctggcgttg cgtaaatcat gtcaacatca ccagattcta
    38341 aagcagctag tgctgtattt tcatcaagta atacccaagt ccacttttta aaataaggct
    38401 ttttaccgtg ccaatatgga tttcgaacga agattgcttg ttctccagcc ttatattctt
    38461 ttaccatgta aggtcctgaa ccgataggat tgctcttata cttatcattg taatgttttt
    38521 tagggacgat tgggatttct gtcaactgtg ctgtaaatgt cgaatgcgcc tcagtcaaat
    38581 gaatatttac ctggtttttc ccaactactt caacattctt aataaaggtc aaatcccaag
    38641 cttttccatc tgctttcaac atatcataag taaacttaac atcgtcagca gtaacaggct
    38701 caccattgga gaatttaaaa tcatcattta agtcgaacga ccacgttaag ccatccttag
    38761 agattttata ttttttagca agctctcctt ttatatctag ttcaggagaa cgtttcaata
    38821 gggtactatg agtaatatta ccttcattat ggattccata acggtccttt ggatcgaatt
    38881 catgaggaag ctttgccccc atagaaacaa ccaattcatc tttgggtcgt tgttttcgtg
    38941 ttcgctcctt tgtttgtgaa ttttgatttt gacaagctac taaaaagata ctcgccagaa
    39001 acaaagcaac aaaagagatg tacttcaaat atttagtcac tattaaacct ccttttttaa
    39061 ttgataaata atatttttga caactttttt cattataaca agaaaaaagt aggagaacaa
    39121 ttacttaact ggttaattag aaaatttgtt attttatcat taataactta aagttaggta
    39181 aaacacaatt ctagactcta gctcataaac catgttaatc caatgtattt taatagacat
    39241 cataaaaagg ctgaataaat tcagccttaa ccacgctatt agttatagca aaccaatcaa
    39301 atgactaaag ataggaataa ccaaatcaac taccaaaccg ataaccacca ccgctatagc
    39361 tgccatcgca ccttgcagtt ctcccaattc caatgcaaag gctgctccga ctgtatgacc
    39421 agaggtccct agacctaatc ctgcaccaat cttactatca ttcaaatgga aaaatgacac
    39481 taatttttta cctaatgcat aaataataac tgcatttaag atacacgcca ttgctgtcac
    39541 agctgtgttc cctccaattg ctgctgttat tggaagagca attgctgttg ttgctgcttg
    39601 tggcaacatt gaagcaattc cgacttgact aagcccaacc attttagaaa tagcgacgat
    39661 aagcataagg gaaacaatca taccgattac taaactgctg agaatttcta cccaatattt
    39721 tttaacaacg tcatttttct tatagagagg aactgcaaaa gcaattgttg ctggatttaa
    39781 aaaccaaaat attaaatcgc cacctggttt ataagcttgt gtataaaatg tcttaacatc
    39841 ggttcctaaa ccttttgaca taagccaaag aatgacaata ccactaacca ttgcaaagaa
    39901 aagaggctgc aaaaggaaga aacctttact cttcttaaat aaatgttgtc ctatcgtata
    39961 gagtattaaa gaaaagcaaa taccaaagat gggtgttttt aagagttcca tattattttt
    40021 cctctacttt cttactatag tggttaggta atatcttttt aaataaatct gtgacactga
    40081 gttctctgat agcaagtatt aatcgtgcaa cgtatgctac cactactaac ataacaacag
    40141 ttgagattgt aataacagca actagttgca agccttcagc ttttaggata tcaaggttcg
    40201 ctgctactga tattcctgag gggacaaaca taaaaccaat catgcttatc atcaaagccc
    40261 caaaagagtc aacccactct accttgataa ttttagcagt tagcaatacg tacattagca
    40321 ccaaaccaat aaccgtcgtt gggattggta gtgatttcgg taataacatt gaaatcattt
    40381 gtgaaactaa tagaatagct gcatatattg acatttgaat aaacattgga gcttcttttg
    40441 ttttggtttc tttactggtg tgtttcatag tcactacccc ttccttttat tacaatgttt
    40501 taacttgttt tattgatagc tatattgtac ttcaaaataa ataaaacgtt ttcattttca
    40561 gcctcagtta ccaaaaaaag aagccgaatt gcaaattcaa gcttctaaac aacaaaggga
    40621 agttcgcaaa agcactttta catggttaaa tgctcattta ggtcttttaa atacgatcga
    40681 cctactggaa atttctctcc attagacata attagtaaca aagtatgatt aaaccaaggt
    40741 tgaatttcct caatcatctc tagattaatc actgtgtttc gatgaatctg gagaaaatat
    40801 ggtgctacag cgcgtgactt aaaccaattg agtgttttcc gaatagtgta agttttctga
    40861 actgtgctaa cagttaattc gccatcttca acactagctg caacgatatc ttgcatttta
    40921 acaacaacac tgcgatctga taatgtcaag gttaacaatt caacactagc ttttttaggt
    40981 attgcttgct ctacatctga agcagtggtg agttggctca atttcttcac ttttgataga
    41041 gccatatcaa cgcgctgttg ttcaaacggt ttcatgatat aatcaacagc gttactttca
    41101 aaagctttca cagcgtagtt atcataagct gtcgcaaaaa caacaagtgg gggatgtgca
    41161 agttggctca actgattagc caaagtaaag ccattttctt cactaagaga aatatctaaa
    41221 aaaattaaat caatttgctg tctaaataag attttttcag cttcactgat atcctcagcc
    41281 tgaaaaatct cagggttgtc aacctcctgg ctatgttcaa ctaaaaacga taattcttgt
    41341 ctggcaaaca tttcatcatc aagaattaaa attttcatgc tcatcctccc ttattctatt
    41401 aggtattcga taccaaactt ttgtaccatt cttgtcgctc gaaaaatgaa ggcaacttac
    41461 actaccatat aataaattca gcctgttatt tagattaact agagcagtac ctgtaccctt
    41521 actctctgca actgtttctt gacctaattt atcaatgata gtatctgaga ttccttgtcc
    41581 attgtcacta acagaaacac aataataatg accatctggc tttatttgaa ccaatatatg
    41641 gttgtccgtc ttacgttctt tgaaagcatg tctaactgca ttctctacca gtacctgtaa
    41701 accaaaaggc ggtaacttca ttttttctgg tgcactaata tcataagata actgatattt
    41761 atcagggaaa cgtaattttt caacattcat ataagcatcc acatgtgatt tttcttgctc
    41821 aagcgttacc tcacgatcct gaccaccttg caaacttgtt ctaaaaaaag tacttaactg
    41881 catcagtgca taacgtgctt tatcagaatc aatacggatt aatgcactaa ttgtgttaat
    41941 ggcattaaag aagaaatgag ggttgatttg tgcttgtaaa gcctttatct ctgccatact
    42001 ggctaactta ttttgttcct ctgttatccc cattgccagt tgtcctgaaa atatttgcgc
    42061 taaaccaagg actaggtttt cctccacctc agacattgtc ttatctcctg caaagtacat
    42121 ttttaaggca cccacagttt tatcatttat ttttagagga actacaatag cagaatttaa
    42181 ctgacagttg tgatctggac aagaaatcgc cgctttatct tgcgcaattc ttggttcgcc
    42241 atcaaaaata acacttttag ataggtctgt tttgactggt tgtcctgcaa tatggtgatc
    42301 atggccaaca ccaatatgag ctaatacgtt tgaccgatct gttaatccta cagcatcaaa
    42361 gttagtatgc ctctttataa tttcgcaaac gctcctagca gattgcggtg tcaaaccttg
    42421 tctgaggtag ggcagagtct gtcgagtcaa ttcaagaaca tctctcgttt gaactgcgcg
    42481 taactgactt tcatttgaca aataagtttt caaaatcgca aggaaaagtg tggaacctaa
    42541 actatttaaa atcatcattg gaatgacaat cattttgaca agttcccatc ctgtaaaaat
    42601 accaacaaat agcatctgga tactttcggc aataatacta attaaaataa cttggcttgt
    42661 tgaagggtag agatggtttt ccttaagctt atcaccaatc tttccgctaa caatgccgac
    42721 tagaactgaa ctgacaatat agaaagaacc tgaaaagctt ccttgaaaaa agcgatgaac
    42781 tcctccaata aaaccaacaa ttgatccaac cagaggtcca ccaaccaaac ttgccgttgt
    42841 aataactaaa gtccttgtat tagcaagtga gtcagaatgg gaaatcgttg ttagaaaagg
    42901 gcgctcgacc aaacttcgat ccccttttat ttcaattcct gttatattag atataataac
    42961 aaacaagccg aaaatgatga caagtactac cgtttcacgt ttagaccgct cttcaattaa
    43021 ctgtctgaaa taactattat ttaccaataa aaaggctaaa atcataataa ttcctagcct
    43081 ttggaataac aacaccatca acatatgctt atcctcctca agataccatt ttactccgga
    43141 agataaatat agatatcttg ataatctgaa ataaccttgt ctgccattga taaatcttgc
    43201 ggtggataat caggattagc aaagccaaag caatacattc ctgctgcctt agcagctaaa
    43261 ctcccatttc ttgtatcttc tataactata caaactttag ggtctacatc taaaagctct
    43321 gcagctctaa gaaaaacatc tggtgctggt ttagaagacg atacatcttc tcctgtaacc
    43381 atgtattcaa agcattctgt cacacccaac tcttttagat tacgcttaat atctaccatt
    43441 ggagatgagg acgcaactgc taagcgatag ccatgttggt gtaaccaatg aatcagtcgc
    43501 tgagctcctt tgatgggcct aacaccatca cgcgccacaa tcgcttgtcg acgtctgttc
    43561 atctcagcaa tatactcttt tactgttttc ggtaagccaa actcctcttt catagcttgc
    43621 cacatgaatt caaaagtagt ccccatgtac tggtattgat acgagacgtc cgtatcaata
    43681 ccctcctcac gtaacatttc tgtcttatta tctaaaaacg tgtactcgga atcaacaata
    43741 acgccgtcca tatcaaaaat aatgactttt tccatcttaa acctgctttc tatccccttt
    43801 attgtaatcg tttgcctttc tattttcaat aaaaaaaggt tgctcccaca gaaagcaacc
    43861 agtatattta gaaatgatat tttgagagtg attttattta aaaggggagc tcctcttctt
    43921 ccaatactaa atctgacaaa tcaccagaaa tgttattttc acgcatagca cgttgggaac
    43981 ggctttctag caactgaaat gatgatgcta atacttcagt gatatagtgc gtttgaccat
    44041 ccttttcgta cttgcgcgta cgcaattcac catctactga aattaaagag ccctttgtcc
    44101 catagctcgc aagggtttcc gctagacgac cccacataac cacattaata aaatctgctt
    44161 cacgctcacc attacttcct ttaaagcgtc tattaacagc aacagttgca cgcgttactg
    44221 acttgtcagt tggtgttttt accatctcag gctttgctgt tagacgccca atcataataa
    44281 ctttattata cattgactac ctcctcacta ttattattcg aaaataagga agaaaaatgc
    44341 taaaatatat gaaattgttc tgggagaatt aagatgacaa atgtaactag ttataaagaa
    44401 atgctggcta aaccttgggg aaaaatacaa tatgaaataa cttttgctca attaagccat
    44461 atcaaaaatc agaacgtttt agattttgga gctggttttt gtctcaccgc acaacatcta
    44521 gcaaaagaaa acaatgtaac tgctattgaa cccaatccta aactgcttta cgataatcaa
    44581 agtgacaata tttataaaat cctaggttcc tacgaagcat taagagattt gcctaatcaa
    44641 agttttgata ctattatttg tcataatgtt cttgaatata tagacaaaca taatcatcct
    44701 gcttattttg acgaattttc tcgacttcta aaacctaatg gagaactatc tctcatcaaa
    44761 cataatataa ccggtaaaat attacaatca gttattttta gcaatgatac ctccactgcg
    44821 atggaactct taacaggaga agcaaacttt aaaagtgcca gttttgacca gggaaatatt
    44881 tataccttag aagaactaaa acaaaataca aatttattag ttgaacgtta tcagggtatt
    44941 cgtaccttct attcattaca accaaaccat tttaaaactg aaactggttg gctaaacaaa
    45001 atgcttgcta ttgagctaag tgttgctgac aaagctcctt ataaagatat tgcttttttg
    45061 caacacatca cacttaaaaa gtcattgtaa cttcttaagt ttacaactaa aaatcaactt
    45121 agctgaagct aagttgattt ttactaattt ttccaatgtt tattggcgtc gaaagactct
    45181 ccaacaatta cctgatcaga taattcaatg atacctctga cttgaggagc atttggaaga
    45241 gctaactcgc gagcactaca taacattcca aagctatctt ctccacgaag ttttcctggg
    45301 aaaataaggc taccatttgg catcatagct cctggaagag ctgcaactgt tttaagacct
    45361 actgaagcat taggtgcccc acaaacgatt tgaaccgtct tcccatcgtt gatttctact
    45421 tgacagatgt gtaaatggtc actatctgga tgatcaatca tttctttaat ttgtgccaca
    45481 acaaatgtag gttcaatatt attaacaaga gagtcttcga atccttcttt cagtagttct
    45541 gcattaagga ttttaatatc ttgatcagat aaagttattt gacctgctcc ctcaataaca
    45601 attaaacttg acacttcaaa gatattccaa gcaactgttt ctttactatc ttgcagatag
    45661 acgcgtgcta cttgtccgcg acgatcaaca tctagcttag ctccttgact atccttaaca
    45721 ataaccatta aggtatcacc aacatgttct ctattataag taaaaatcat ttattctcct
    45781 ttaaattttg ctattttctg tcttatttat ggttaatttc agcgagaaat ttagtaattt
    45841 cagcctttgt tttgcgattc ttattaacca aacgacccag ttcctgacca ttttccacaa
    45901 caacgaaact cggaatccca aaaatattcc actgctgagc taattctata tagtcatcac
    45961 gattaacacg tacaaagaca aaatctgaga aatctttttc aattgatggc attactggat
    46021 atataaattg acaatctgga caccaatctg ctgtaaaaaa gaaaacaact ttcttagtgc
    46081 tatcaatata ggctgctatt tcttcataag attctagtaa aatcatttac tcacctccac
    46141 ataatcaaga acaccttgac gataaatgaa ttcgaaaatt ctgccatctt ccaagacaag
    46201 accaccactg gtcattttaa tatcagattc aaattcatta aaataaaata cctgaatatc
    46261 acccatctga ctaaaaaatt ctcttaaggt ctgagtgatt tgctgacgtt tataatctgt
    46321 tactttttta gttagggtat aaccagcagc tgccagtaaa cccacaccag caaggccagt
    46381 cccaaataag atttttttat ttttcataac tgttattata ccataccccc cggaattaga
    46441 cacaggaatt gatggaagaa atccacataa cagttatgaa aaaaaacaac tcattccttg
    46501 tggtataata ggatgacatt gtcttaagat accattatgg ttaatcattc aaacagaaag
    46561 aaaaaacatg aaagaaaaat tattagtacc tgctttaaat tgtttgctag gtctactctt
    46621 cctaggtgat cactggtttt tatttcgtct tcaaaatgga gaactttttg ctattgtgac
    46681 gctaatactg ctttgcacaa cttcattttt aatcaataag aatcacccca aaaatttgac
    46741 tttgatttct atgattatca cttttctagc cgtattatct ttcctatcta cctttttact
    46801 agcttaagcc gataaacatt atctccgttg tattcaaccc agttttcact ataacaggct
    46861 ttgtcaatca taacagtctt ctggtagtac ttgatattct ttttgaaagt ctttatattg
    46921 actgaaacct gttctgttct ttatttttaa ttaaaaggtg ctaaaatagt atttgtagaa
    46981 ttgaggtaat tatgtcagat ttatttaaca aaattaaaac cgtaactgag cttgatggga
    47041 ttgctggcta tgaacacaat atccgcaact tccttcgtca agaaataact cctttagttg
    47101 atcaagttga gacagacgga cttggtggaa tttttggagt taaaaatact catgagacta
    47161 atgctcctaa agtcatggtt gctgcccata tggatgaagt cggctttatg gttagtcata
    47221 ttcagccaga tggaacattt cgtgtacttg aggttggagg atggaatccc ctagtagtca
    47281 gctcacaacg ctttaccctc tacacacgtt ctggtgatgc tattcctgtt atatcaggct
    47341 cagttcctcc tcactttctt cgtggacaaa gcggtggaac gacattaccc aaaattagtg
    47401 acattgtttt tgatggagga ttcacagata aaaatgaagc tgaaagcttt ggcattgctc
    47461 ctggcgatat cattgttcct aaatctgaaa ccattttaac tgcaaatcaa aaacatatta
    47521 tgtcaaaagc ttgggataat cgctatggtg tgcttatggt gaccgaattg ctaaaaagct
    47581 taaaagatca aagtcttagc aacacactta ttgctggggc aaatgttcaa gaagaagtcg
    47641 gacttcgtgg cgcacatgtt tcaacaacta aattcaaccc agatatcttc ttagctgtcg
    47701 attgttcccc agctggagat atttatgggg aacaaggcaa aataggagag ggaaccttaa
    47761 tccgttttta tgatcccgga catatcatgc ttaaagatat gagagatttc ttgcttacaa
    47821 cagctgaaga agcaggtata aaataccaat attatgctgc aaaaggtggt accgatgctg
    47881 gggctgctca cctaaaaaat agtggtattc cttctacaac tatcggtgtc tgtgcacgct
    47941 acattcattc tcatcaaaca ctctacgcta tggatgattt tctacaagca caagcttacc
    48001 ttcaggctat cgttaacaaa ttagaccgct cgacggtgga tattattaaa ggttattaag
    48061 atagaaagga tgggagtttc ataaggtaga cacattttag tatctccttt catccccata
    48121 tcaacattat gaaaattgga attatcggcg ttggaaaaat ggccagtgca attattcaag
    48181 gcctcaaaca aacccaacat gacattgtca tatccggatc ttgcttagag cgttcaaaag
    48241 aaattgcaga aagacttgat gttacttacg ccgaatctca tcagtcatta atcaatcaag
    48301 ctgatattat catgttaggt attaaacctc aactatttga aaaggttctt cttccacttg
    48361 atatcacaaa accaatcatc tcaatggcag caggaatctc cttagcgcgt ttaagccaat
    48421 taactcgttc tgatttacca ttgattcgta ttatgccaaa tattaacgca caaatacttc
    48481 aaagttgcac agctatttgt tacaacaatc atgtatcgga tgagttacga caattaacca
    48541 aagaaattac ggatagtttt ggaagtagct ttgatatagc agaaaccaat tttgatacct
    48601 tcactgctct agcaggttct agccctgcct atatctatct atttatagaa gccttagcta
    48661 aagcaggtgt aaaatatgga tttccaaaag aacaagcact ctctattgta ggacagactg
    48721 tcttagcttc tagtcaaaac ctattacaag gacaaaatag cacctctgac ttaatcgaca
    48781 acatttgtag cccaggcgga actactattg ctggcctttt agacttagaa aaaaatggtc
    48841 tgacgcatag tgttatttct gccattgatg ctactattga gaaagcaaaa aaactttgag
    48901 caaattgctc aaagtttttt tagttattca tcattgtact gattaacacg ctataagcaa
    48961 gagcattgtt aataaaatga accattatac tatattctaa gtgctcagtt ttataataaa
    49021 caaaagataa aatagcaccc atcccagcat aaataataaa actacctata tcagaagggg
    49081 aatgaataag agcaaaagct aaagtaccga tgacaaaacc ccatttttga tgtttcgcaa
    49141 aaagttcttt agggataata tagcgaaata taatttcttc cataataggg gcaccaatta
    49201 tggtaaaaat taacatcaaa gctagtggaa gttgctttgt taattccata attactgttt
    49261 gatttgctgt atctttaacc ccttgcatag tcataatgag gtaaccaata atttgtggaa
    49321 ccctcaatga taaccaacct aagaaaatag taactaatgc tttccttgtc aataatgctt
    49381 tccatctaat tatttgttta accttggcat aataaaggaa aagagcaata ataagtaatt
    49441 ctaagccaat taacaatata ctagtaaaag gcgtttgata gtgattcttt a
//
LOCUS       DATRTN010000009        43122 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.009, whole genome shotgun sequence.
ACCESSION   DATRTN010000009 DATRTN010000000
VERSION     DATRTN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43122
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.009"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..468
                     /locus_tag="VBE22_000512"
     CDS             <1..468
                     /locus_tag="VBE22_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEO7201988.1"
                     /translation="CWLCAGTLRNFIWNKLSGINETLTSDIDVVFFDKNISYEETVVL
                     EQQLKDNYPQYDWELKNEFYMNTHSPNTPKYTSSKDAISKFPEKCTAVGARLDDRNQL
                     ELYLPYGEEEILNFIVSPTPYFEEDLLRYNVYLKRVDKKKWNNIWPRLTIIKK"
     gene            688..1107
                     /locus_tag="VBE22_000513"
     CDS             688..1107
                     /locus_tag="VBE22_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HEO7201989.1"
                     /translation="MKMTVYFDGNFWLGLIEYDDDGDYKVFRYFFGKEPKDDDVFNFI
                     NHKLNDLIKKYEFVKTDISLKRTNEHKKSPKRMQREINREKRKPVVSTKAQLAMKTIH
                     MSIKNERQLSQKCKKNELRKHRYQLKQEKRYQKKKGH"
     gene            1307..1786
                     /locus_tag="VBE22_000514"
     CDS             1307..1786
                     /locus_tag="VBE22_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7201990.1"
                     /translation="MRDFEELFDKVKPIVMKLRRNYFVQLWEYDDWIQEGRIVLFRLL
                     EEHPYLLDNESKLFIYFKTKFSNYLNDVLRHQDCQKRQFNKMPYEEISEVSHYVKSKG
                     LVLDDYIAYRDTLTKVEETLSDIDKEKFEKLISGERFAGKKQFIRDIQPFFNAFKAD"
     gene            1908..2600
                     /locus_tag="VBE22_000515"
     CDS             1908..2600
                     /locus_tag="VBE22_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO7201991.1"
                     /translation="MSKVRLYIARHGKTMFNTIGRAQGWSDTPLTTFGELGIKELGLG
                     LKASNISFKEAFSSDSGRTLQTMEIILREVQQENIPYTRDKRIREWCFGSLDGGYDGD
                     LFNGVLPRVSNGDMSHLTHEEIANLICQVDTAGWAEPWAILSNRILSGFTAIAKKIED
                     IGGGNAIVVSHGMTIATFLWLIDHSTPRSLGIDNGSVSVVDFEDGTFSIQSIGDMSYR
                     EKGREILEKTLQ"
     gene            2597..3349
                     /locus_tag="VBE22_000516"
     CDS             2597..3349
                     /locus_tag="VBE22_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEO7201992.1"
                     /translation="MKKNKIIRFSLVGVLLAILCFSLFALLKPNSQQSSSQKLRNEDI
                     KKISSQKRNKKLQLPAVSSKDWNLILVNRDHKHEELSPDVVPVENIYLDKRITKQATQ
                     FLEAARAIDSREHLISGYRSVAYQEKLFNSYVTQEMTSNPNLTRGQAEKLVKTYSQPA
                     GASEHQTGLAMDMSTVDSLNESDPRVVSQLKKIAPQYGFVLRFPDGKTAETGVGYEDW
                     HYRYVGVESAKYMAKHHLTLEEYITLLKENNQ"
     gene            3346..3921
                     /locus_tag="VBE22_000517"
     CDS             3346..3921
                     /locus_tag="VBE22_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEO7201993.1"
                     /translation="MRKRFSLLNFIVVTFIFFFFILFPLLNHKGKVDANSRQSVTYTK
                     EEFIQKIVPDAQDLGKSYGIRPSFIIAQAALDSDFGEKILANKYHNLFGLLAEPGTPS
                     ITLNDSSTGKKQEKQFTHYKSWKYSMYDYLAHIKSGATGKKDSYTIMVSVKNPKTLVQ
                     KLQDSGFDNDKKYAKKMTEIIDLYDLTRYDK"
     gene            4016..5098
                     /gene="hrcA"
                     /locus_tag="VBE22_000518"
     CDS             4016..5098
                     /gene="hrcA"
                     /locus_tag="VBE22_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEO7201994.1"
                     /translation="MCKSANFRKTERGDVFVITQRQNDILNLIVELFTQTHEPVGSKA
                     LQRTIESSSATIRNDMAKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQD
                     IYHVIKAFDFEAFKLEDMLQKASHILSEMTGYTSVILDVEPARQRLTGFDVVQLSNHD
                     ALAVMTLDESKPVTVQFAIPRNFLTRDLIAFKAIVEERLLDGSVMDIHYKLRTEIPQI
                     VQKYFVTTDNVLQLFDYVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLR
                     QGLKEGEMASVQVADSQEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLV
                     NIIGKVLAAKLGDYYRYLNSNHYEVH"
     gene            5101..5673
                     /gene="grpE"
                     /locus_tag="VBE22_000519"
     CDS             5101..5673
                     /gene="grpE"
                     /locus_tag="VBE22_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEO7201995.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEEIP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            5854..7683
                     /gene="dnaK"
                     /locus_tag="VBE22_000520"
     CDS             5854..7683
                     /gene="dnaK"
                     /locus_tag="VBE22_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEO7201996.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVEEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIQSNSGLTDEEIDKMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKKAQESGNLDDMKAKLEALNEKAQ
                     ALAVKLYEQAAAAQQAAQGAEGAQSADSSSKGDDVVDGEFTEK"
     gene            7972..9111
                     /gene="dnaJ"
                     /locus_tag="VBE22_000521"
     CDS             7972..9111
                     /gene="dnaJ"
                     /locus_tag="VBE22_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEO7201997.1"
                     /translation="MNNTEFYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKETGAEE
                     KYKEVQEAYETLSDTQKRAAYDQYGAAGANGGFGGFDGGGFGGFDGGGFGGFEDIFSS
                     FFGGGGMRNPNAPRQGDDLQYRVNLSFEEAIFGAEKEVSYNRESSCHTCSGSGAKPGT
                     SPVTCQKCHGSGVINVDTQTPLGTMRRQVTCDVCQGSGQEIKEKCPTCHGTGHEKKTH
                     KVSVKIPAGVETGQQIRLTGQGEAGFNGGPYGDLFVIINVLPSQQFERNGSTIYYTLK
                     ISFVQAALGDTIDIPTVHGAVEMSIPAGTQTGKTFRLRGKGAPKLRGGGQGDQHVTVN
                     IVTPTKLNDAQKEALHAFAEASGDKMVHPKKKGFFDKVKDALDVD"
     gene            9229..10476
                     /locus_tag="VBE22_000522"
     CDS             9229..10476
                     /locus_tag="VBE22_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEO7201998.1"
                     /translation="MVTKVEEIRSYLIASIQNGKLAPGDRLPSIRQLANQFSCNKDTV
                     QRVLMELRFDNYVYAKPRSGYYVFDSHEEEVEEGVSLPNSEIANIAYDDFRLCLNETL
                     IGREDYLFNYYYRQEGLLDLSKAVAKLMEETGVYVPLDDIVITAGTQQALFILTQVTF
                     PNRKSRVLIEEPTYPRMIELIKTQNLPYETISRGTHGIDFQRLEEIFQTQSIKFFYVI
                     PRMHNPLGTSYNPVEMKRLIEMAEKYDVYIVEDDYMSDFASQSPLHYYDTHGRVIYLK
                     SFSKAIFPALRLAAICLPQALKSTFMAYKKLMDYDTNLILQKALALYIENGLYGKNSQ
                     YLKYRYQKDLANSKSILADQPNLPPYSLHHDSVLFDCSKLDNFKILRQYGDTLENYFC
                     QKSHQSLLQVKNDSCLKQFLGSL"
     gene            10547..11323
                     /gene="truA"
                     /locus_tag="VBE22_000523"
     CDS             10547..11323
                     /gene="truA"
                     /locus_tag="VBE22_000523"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEO7201999.1"
                     /translation="MTRYKAQISYDGSAFSGFQRQPNCRTVQEEIERTLKRLNSGNDV
                     IIHGAGRTDAGVHAYGQVIHFDLPQARDVEKLRFGLDTQCPDDIDIVKVEQVSDDFHC
                     RYDKHIKTYEFLVDIGRPKNPMMRNYATHYPYPVIIELMQEAIKDLVGTHDFTGFTAS
                     GTSVENKVRTIFDAKIQFEASKNLLIFTFTGNGFLYKQVRNMVGTLLKIGNGRMPISQ
                     IKTILQAKNRDLAGPTAAGNGLYLKEIIYEDKECFSNFRK"
     gene            11286..12044
                     /locus_tag="VBE22_000524"
     CDS             11286..12044
                     /locus_tag="VBE22_000524"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO7202000.1"
                     /translation="MKTRNVLAISGNDIFSGGGLHADLATYVVNKLHGFVAVTCLTAM
                     SDKGFEVIPIEASILKQQLESLKDVEFGSIKLGLLPNVETAQVVLEFVKSKQECPVVL
                     DPVLVCKENHDLEVSQLREQLIAFFPYADVITPNLVEAQLLTGLSIENLDQMKIAAEK
                     LYDMGAKHVVIKGGNRLNAEEATDLYYDGERFETYVFPVVDANNTGAGCTFASSIASQ
                     LAMGKNVEDAVKMSKGFVYQAIKASDKYGVVQHF"
     gene            12054..12518
                     /locus_tag="VBE22_000525"
     CDS             12054..12518
                     /locus_tag="VBE22_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7202001.1"
                     /translation="MRKEKTSQLVQLAIVTAISIVLGMFISIPTPTGFLTLLDAGIFF
                     AAFYFGKKEGAVVGALAGFLIDLLKGYPNWMFFSLLIHGAQGYLAGLPGRRRLLGLIS
                     ATLVMVLGYAIASGLMYGWGAVLPDIPGNIMQNMVGMVVGFALNKSLERVKK"
     gene            12515..13084
                     /locus_tag="VBE22_000526"
     CDS             12515..13084
                     /locus_tag="VBE22_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEO7202002.1"
                     /translation="MILDYNKLKQETKAIVVDIIERSALKKGQIFVLGLSSSEVSGGL
                     IGKNSSSEIGEIIVEVILKELHSRGIYLAVQGCEHVNRALVVEAELAERQQLEVVNVV
                     PNLHAGGSGQVAAFKLMTSPVEVEEIVAHAGIDIGDTSIGMHIKRVQVPLIPISRELG
                     GAHVTALASRPKLIGGARAGYTSDPIRKF"
     gene            complement(13121..13963)
                     /locus_tag="VBE22_000527"
     CDS             complement(13121..13963)
                     /locus_tag="VBE22_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEO7202003.1"
                     /translation="MITLEKFIDHLNVEEVLFTFFTKLISILLLIIAFVIVRQVINYL
                     FEKTVNRSLAFSRQKVARQKTLAKLSHNVLNYTLYFFLFYWILSLLGVPISSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILLENQFDVGDIINVGTVSGTVTNVGIRTTQIHDFD
                     GTLHFIPNRNITIVSNKSRSNMRAQIDIPLFVHTNLDQISDIVTKINEEYVSKHPAIV
                     GEPTVFGPTTNANGQFVYRINIFTQNGAQFDIYAEFYKLYQKAILEEGIDLPTYNFSN
                     NHSR"
     gene            14120..15403
                     /locus_tag="VBE22_000528"
     CDS             14120..15403
                     /locus_tag="VBE22_000528"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEO7202004.1"
                     /translation="MSTSFENKATNRGIITFTISQDEIKPALDQAFNKVKKDLNVPGF
                     RKGHMPRTVFNQKFGEEALYENALNLVLPKAYEAAVAELGLDVVAQPKIDVVSMEKGQ
                     DWKLTAEVVTKPEVKLGDYKDLSVEVDASKEVSAEEVDAKVERERNNLAELTVKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEEQLVGSKAGQTVDVNVT
                     FPEDYQAEDLAGKDAKFVTTIHEVKTKEVPALDDELAKDIDDEVETLDELKAKYRKEL
                     ESAKEIAFDDAVEGAAIELAVANAEIVELPEEMVHDEVHRAMNEFMGNMQRQGISPEM
                     YFQLTGTTEEDLHKQYQADADKRVKTNLVIEAIAAAEGFEATDEEIEKEITDLASEYN
                     MEADQVRGLLSADMLKHDIAMKKAVDVITSSATVK"
     gene            15592..16167
                     /locus_tag="VBE22_000529"
     CDS             15592..16167
                     /locus_tag="VBE22_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEO7202005.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILEQRGRDNEMYFSDLVNDIQT
                     YLGKSDSAIRESLPFFYSDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     APKRKKKRVNAFMDGDEDAIDYNDDDPEDEDFTEETPSLEYDEENPDDEKSEVESYDS
                     EINEIIPDEDLDEDVEINEEDDEEEEEEEEV"
     gene            16440..18044
                     /locus_tag="VBE22_000530"
     CDS             16440..18044
                     /locus_tag="VBE22_000530"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEO7202006.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLKKE
                     RRGEYLGATVQVIPHVTDALKEKIKRAATTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QSIKNKLAQFCDVAPEAVIESLDVDHIYQIPLNMQAQNMDQIVCDHLKLETPAADMTE
                     WSAMVDKVMNLEKKVKIALVGKYVELPDAYLSVVEALKHSGYVNDVAIDLKWVNAAEV
                     TEDNIKELVGDADGIIVPGGFGQRGSEGKIEAIRYARENDVPMLGVCLGMQLTCVEFA
                     RNVLKLHGANSAELDPKTPFPIIDIMRDQIDIEDMGGTLRLGLYPCKLKAGSRAAAAY
                     NNQEVVQRRHRHRYEFNTKFREQFEAAGFVFSGVSPDNRLMEVVELPEKKFFVAAQYH
                     PELQSRPNHAEELYTAFVTAAVENMK"
     gene            18153..19079
                     /locus_tag="VBE22_000531"
     CDS             18153..19079
                     /locus_tag="VBE22_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7202007.1"
                     /translation="MKKIRLSKFIKMIVVILFLISVAASFYFFHVAQVRDDKSFISNG
                     QRKPGNSLYAYDKSFDKLLKQKIEMTNQNIKQVAWYVPAAKKTHKTAVVVHGFANSKE
                     NMKAYGWLFHKLGYNVLMPDNIAHGESHGQLIGYGWNDRENIIKWTEMIVDKNPSSQI
                     TLFGVSMGGATVMMASGEKLPSQVVNIIEDCGYSSVWDELKFQAKEMYGLPAFPLLYE
                     VSTISKIRAGFSYGQASSVEQLKKNNLPALFIHGDKDNFVPTSMVYDNYKATAGKKEL
                     YIVKGAKHAKSFETEPEKYEKRISSFLKKYEK"
     gene            19126..19211
                     /locus_tag="VBE22_000532"
     tRNA            19126..19211
                     /locus_tag="VBE22_000532"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19160..19162,aa:Leu,seq:aag)
     gene            19274..19720
                     /locus_tag="VBE22_000533"
     CDS             19274..19720
                     /locus_tag="VBE22_000533"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEO7202008.1"
                     /translation="MSKVRGFELVSQFSNKELLPKRETAHAAGYDLKVAKKTVIEPGE
                     ITLVPTGIKAHMQPGEVLYLYDRSSNPRKKGIVLINSVGVIDGDYYNNQVNEGHIFAQ
                     MQNITDQAVILEEGERIVQAVFAPFLLADDDQATGMRTGGFGSTGK"
     gene            19882..21246
                     /gene="radA"
                     /locus_tag="VBE22_000534"
     CDS             19882..21246
                     /gene="radA"
                     /locus_tag="VBE22_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEO7202009.1"
                     /translation="MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKN
                     ARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
                     KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAKRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPKESFIGE
                     IGLTGEIRRVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKA
                     VFST"
     gene            21382..21879
                     /locus_tag="VBE22_000535"
     CDS             21382..21879
                     /locus_tag="VBE22_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEO7202010.1"
                     /translation="MTTYFDNFLKTNQAYADLHGTAHLPIKPKTKVAIVTCMDSRLHV
                     AQALGLDLGDAHILRNAGGRVTDDVLRSLVISQQQLGTREIVVLHHTDCGAQTFTNEA
                     FATQLQRDLGVDMHGHDFLPFNDIEESVREDVAKLHASPLIPNDVVISGAIYDVDTGR
                     MVEVK"
     gene            22033..23352
                     /locus_tag="VBE22_000536"
     CDS             22033..23352
                     /locus_tag="VBE22_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEO7202011.1"
                     /translation="MKKYDVIVLGFGKAGKTLAAKLATQGKSVAMVEEDDKMYGGTCI
                     NIGCIPTKTLLVSASKNHDFQEAMTTRNEVTSRLRAKNFAMLDNKDTVDVYNAKARFI
                     SNKVVELTGGADKQELTADVIIINTGAKSVQLPIPGLADSQHVYDSTAIQELAHLPKR
                     LGIIGGGNIGLEFATLYSELGSKVTVIDSQSRIFAREEEELSEMAQDYLEEMGISFKL
                     SADIKSVQNEDEDVVISFEDEKLSFDAVLYATGRKPNTEGLALENTDIKLTERGAIAV
                     DEYCQTSVENIFAVGDVNGGPQFTYISLDDSRIVLNYLNGDKDYSLKNRGAVPTSTFT
                     NPPLATVGLDEKTAKEKGYQVKSNSLLVSAMPRAHVNNDLRGIFKVVVDTETNLILGA
                     RLFGAESHELINIITMAMDNKIPYTYFQKQIFTHPTMVENFNDLFNF"
     gene            23561..25015
                     /locus_tag="VBE22_000537"
     CDS             23561..25015
                     /locus_tag="VBE22_000537"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEO7202012.1"
                     /translation="MANKIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPETHENYRQSERLELYQRYIDQLLAEGKA
                     YKSYVTEEELAAERERQELAGETPRYINEFIGMSETEKEAYIAEREASGIIPTVRLAV
                     NESGIYKWTDMVKGDIEFEGSNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPQFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     SEAVFNFIALLGWNPGGEEEIFSREQLINLFDENRLSKSPAAFDQKKMDWMSNDYLKN
                     ADFESVFALCKPFLEEAGRLTDKAEKLVELYKPQLKSADEIVPLTDLFFADFPELTEA
                     EKEVMAAETVPTVLSAFKEKLVSLSDEEFTRDTIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIYLLGKEKSVQHIDNMLAKL"
     gene            complement(25055..26023)
                     /locus_tag="VBE22_000538"
     CDS             complement(25055..26023)
                     /locus_tag="VBE22_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO7202013.1"
                     /translation="MKFGKKLGFLALLMSIVLILGACGKTGLGNSTGNSTKNVTKKSA
                     KNLKLGVSISTTNNPYFVAMKDGIDKYASNKKISIKVADAQDDAARQADDVQNFISQN
                     VDAILINPVDSKAIVTAIKSANNANIPVILMDRGSEGGKVLTTVASDNVAAGKMAADY
                     AVKKLGKKAKAFELSGVPGASATVDRGKGFHSVAKSKLDILSSQSANFDRAKALNTTQ
                     NMIQGHKDVQIIFAQNDEMALGAAQAVKSAGLQNVLIVGIDGQPDAHDAIKKGDISAT
                     IAQQPAKMGEIAIQAAIDHYKGKKVEKETISPIYLVTKDNVEKYNW"
     gene            complement(26076..27017)
                     /gene="rbsC"
                     /locus_tag="VBE22_000539"
     CDS             complement(26076..27017)
                     /gene="rbsC"
                     /locus_tag="VBE22_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771495.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEO7202014.1"
                     /translation="MKNMMKYISELTTLIALAGLMVIITLINPNFLTTNNLLNLLLQV
                     TANGFIAFGMTFVILTGGIDLSVGSILALSSALTAGLIGHGVPVGIAIVLSVALGGIL
                     GMLNGLFISYGKLAPFIVTLATMTIFRGATLVYSNGNPITAGLSDSFLFQFLGQGYIV
                     GIPFPVILMFLTFIILYILLHKTAFGKSVYALGGNEKAAYISGIKLNKVKIIIYTISG
                     IMASISGLIITSRLSSAQPTAGASYEMDAIAAVVLGGTSLSGGKGRIIGTLIGALIIG
                     VLNNGLNIIGVSAFWQQVVKGIVILMAVLLDRFKVAK"
     gene            complement(27019..28497)
                     /locus_tag="VBE22_000540"
     CDS             complement(27019..28497)
                     /locus_tag="VBE22_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEO7202015.1"
                     /translation="MKIDMRNISKSFGTNKVLEKIDLELQSGQIHALMGENGAGKSTL
                     MNILTGLFPASTGTIYIDGEERTFSNPQEAEEFGISFIHQEMNTWPEMTVLENLFLGR
                     EIKTTFGLLNQKLMRQKALEAFKRLGVTIPLDIPIGNLSVGQQQMIEIAKSLLNQLSI
                     LVMDEPTAALTDRETENLFRVIRSLKQEGVGIVYISHRMEEIFKITDFVTVMRDGVIV
                     DTKETSLTNSDELVKKMVGRKLEDYYPEKHSEIGPVAFEVSNLCGDNFEDVSFYVRKG
                     EILGFSGLMGAGRTEVMRTIFGIDKKKSGKVKIDNQEITITSPSQAIKQGIGFLTENR
                     KDEGLILDFNIKDNMTLPSTKDFSKHGFFDEKTSTTFVQQLINRLYIKSGRPDLEVGN
                     LSGGNQQKVVLAKWIGIAPKVLILDEPTRGVDVGAKREIYQLMNELADRGVPIVMVSS
                     DLPEILGVSDRIMVMHEGRISGELSRKEADQEKVMQLATGGK"
     gene            complement(28513..28911)
                     /gene="rbsD"
                     /locus_tag="VBE22_000541"
     CDS             complement(28513..28911)
                     /gene="rbsD"
                     /locus_tag="VBE22_000541"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEO7202016.1"
                     /translation="MKKTGILNSHLAKLADDLGHTDRVCIGDLGLPVPNGIPKIDLSL
                     TSGIPSFQEVLDIYLENILVEKVILAEEIKEANPDQLSRLLAKLDNSVSIEYVSHDHL
                     KQMTQDVKAVIRTGENTPYSNIILQSGVII"
     gene            complement(28886..29797)
                     /gene="rbsK"
                     /locus_tag="VBE22_000542"
     CDS             complement(28886..29797)
                     /gene="rbsK"
                     /locus_tag="VBE22_000542"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEO7202017.1"
                     /translation="MSNIVIIGSISMDLVMETNRIAKEGETVFGQRFSMVPGGKGANQ
                     AVAIGRLSQDRDNITILGAIGEDSFGPILLDNLNKNHVTTDFVGTIPSSSGVAQITLY
                     NNDNRIIYCPGANGKVDTKKWSQEWSIIKEADLVVLQNEIPHQANMKIANFCKEHSIK
                     LLYNPAPSRKTDIEMLDKVDYFTPNEHECQELFPNQKLEDILATYPEKLIVTLGTKGA
                     IYSDGKESHLIPALETKAVDTTGAGDTFNGAFGYAISKKFKIANALRFATLAAHLSVQ
                     KFGAQGGMPTIKEMEDNQHYEKDWHIK"
     gene            complement(29790..30776)
                     /locus_tag="VBE22_000543"
     CDS             complement(29790..30776)
                     /locus_tag="VBE22_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7202018.1"
                     /translation="MSTIRQVAEKAGVSTSTVSRYISQNGYVSQKASQKIEQAIRELH
                     YVPNFLAQSLKTKKNQLVGLLLPDISNPFFPRLARGVEEFLKEQGYRVMLGNTNNKSH
                     LEEEYLNVLLQSNAAGIITTHDFTKNHPEIDIPVVVVDRVNQETQYGVFSDNKEGGKL
                     AAQAIWTAGATNILLIRGPLDKADNLNQRFQGSQNYLLNKGARFAIEDSASFDFAEIQ
                     IEAKTLLDHHPDIDSIIAPSDIHAIAYLHEILNRGKRIPEDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYIMGQKAAELIFKITNQLPITNKRIKLPVHYVERETLRRKIDE"
     gene            30958..32046
                     /locus_tag="VBE22_000544"
     CDS             30958..32046
                     /locus_tag="VBE22_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7202019.1"
                     /translation="MSHAWKELTFYKKKYLLIELLIIVMMFMVVFLSGLANGLGRAVS
                     AAIENNPAQTYILNEGAEQVITSSVLTTKDQTDLNSLNLKDSTTLNIQRSSLTRQGHE
                     KKIDISYFAIDKDSFMAPTLSEGKQLTSYKKAIILNDSLKAEGIKLGDKVIDKSSSIS
                     LTVVGFVHNSMYGHGPVAFIDKDIYTEINKKINPQYQFLPQALVMKNDKSISHLPTQL
                     EAVSKKDVIQHIPGYSAEQSTLNMILWVLVVASAGILGVFFYIITLQKRHEFSVMKAI
                     GTKMSEIALFQLSQVIILALFGIIVGDGLAIALSYVLPAQMPFVINWQNIILVSFVFL
                     VIAMISSALSIVKVAKIDPVEVINGGGE"
     gene            32046..32732
                     /locus_tag="VBE22_000545"
                     /pseudo
     CDS             32046..32732
                     /locus_tag="VBE22_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480208.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32763..33434
                     /locus_tag="VBE22_000546"
     CDS             32763..33434
                     /locus_tag="VBE22_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7202020.1"
                     /translation="MIKILVVEDNIVQQKIITTKLTQEGYQFITASNGQEALNCLDTE
                     EVQLIITDIMMPMMDGYQLIQELRSAAYNVPIIVMTAKSQMEDMTKGFGLGADDYMVK
                     PVQLQELALRIKALLRRANIVAQHQLIIGNTCLNEDELSLKYFEQEIVFPQKEFRVLF
                     HLLSYPNRIFTRLELLDSIWGMDTDLDERVVDACINKIRRKVEHLPDFKIETVRGVGY
                     RAKND"
     gene            33418..34497
                     /locus_tag="VBE22_000547"
     CDS             33418..34497
                     /locus_tag="VBE22_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7202021.1"
                     /translation="MQKMTKLRRFRFPLRFYFTLMFVLTMLFSVLASLLLVAAIVFTF
                     FQGVLTTHVLQVSALAVVFLSLVIASISMWYGSYHLTKPILDISRIVSNVADGDFEGH
                     IYRNSNRRKSYEYYNELDELSESINQMIVSLSHMDHMRKDFITNVSHELKTPIAAVAN
                     IVELLQDPELDEETQSELLGLVKTESLRLTRLCDTMLQMSRVDNQETIGELSSVRVDE
                     QIRQAMISLTERWQAKRINFQLDSKPYTVYSNSDLLMQVWINLLDNAIKYSDDIVDLR
                     VRMEETNNHYLRVIISDKGRGISQYDVQHIFDKFYQADQSHNQQGNGLGLAIVKRIIV
                     LCKGRISVSSQFEIGTEFCVELPLS"
     gene            34651..35841
                     /locus_tag="VBE22_000548"
     CDS             34651..35841
                     /locus_tag="VBE22_000548"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEO7202022.1"
                     /translation="MGKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDF
                     IHDKALTIGAIESYILDVKDEFAEHFVLPALQAHAMYEQKYPLVSALSRPIIAQKLVE
                     MAHQTGATTIAHGCTGKGNDQVRFEVAIAVLDPELKVIAPVREWKWHREEEITFAKAN
                     GVPIPADLDNPYSIDQNLWGRANECGVLENPWNQAPEEAFGITKSPEEAPDCAEYIDI
                     TFQNGKPIAINNQEMTLADLILSLNEIAGKHGIGRIDHVENRLVGIKSREIYECPAAM
                     VLLAAHKEIEDLTLVREVSHFKPILENELSNLIYNALWFSPATKAIIAYVKETQKVVN
                     GTTKVKLYKGSAKVVARHSSNSLYDENLATYTAADNFDQDAAVGFIKLWGLPTQVNAQ
                     VNKG"
     gene            35860..37248
                     /gene="argH"
                     /locus_tag="VBE22_000549"
     CDS             35860..37248
                     /gene="argH"
                     /locus_tag="VBE22_000549"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEO7202023.1"
                     /translation="MTKNHKLWGGRFESSLEKWVEEFGASISFDQKLAPYDMKASMAH
                     VTMLGKTDIISQEEAGLIKDGLKILQDKYRAGQLTFSISNEDIHMNIESLLTAEIGEV
                     AGKLHTARSRNDQVATDMHLYLKDKLQEMLKKLLHLRTTLVNLAENHIYTVMPGYTHL
                     QHAQPISFGHHLMAYYNMFTRDTERLEFNMKHTDLSPLGAAALAGTTFPIDRHMTTRL
                     LDFEKPYSNSLDAVSDRDFIIEFLSNASILMMHLSRFCEEIINWCSYEYQFITLSDTF
                     STGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKSLPLAYNKDLQEDKEGMFDS
                     VETVSIAIEIMANMLETMTVNEHIMMTSTETDFSNATELADYLASKGIPFRKAHEIVG
                     KLVLECSKNGSYLQDIPLKYYQEISELIENDIYEILTAKTAVKRRNSLGGTGFDQVKE
                     QILLARKELKAE"
     gene            37389..38270
                     /locus_tag="VBE22_000550"
     CDS             37389..38270
                     /locus_tag="VBE22_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEO7202024.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKLCKQLIETLVESMGITVPVAIHLDHGHYDDALECIEVGYTS
                     IMFDGSHLPVEENLEKAREVVAKAHAKGISVEAEVGTIGGEEDGIVGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPANWEGLDLDHLKKLTEAVPGFPIVLHGGSGIPDDQI
                     QEAIKLGVAKVNVNTECQLAFCQATRDYAVEFNANEAEYMKKKLFDPRKFLKPGFDAI
                     TEAVEERIDVFGSANKA"
     gene            complement(38355..39272)
                     /locus_tag="VBE22_000551"
     CDS             complement(38355..39272)
                     /locus_tag="VBE22_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO7202025.1"
                     /translation="MTRKIGIIGMGHVGSTVAHALIAQGIGDDYVFIDTNIDKVNADM
                     IDFQDALANATYHANIVLNDYDALSDADVVISALGDISLQNNKEANRFAELPFTSKEV
                     YDVSQKLKSSGFRGILIVVTNPVDAITQLYQEYTQFPKSRVIGTGTLLDTARMKRVLG
                     QRLQVDPRSIGGYNLGEQGNSQFVAWSQVTVKGQPAISLLKAEELEQIKEASLKGGHT
                     VFFGKKYTSYGIATAAVRIVLAVLNDSHELLPVSTFYESEGVYLGYPAIIGRVGIVER
                     ISLSLSPKEEQQLTDSAAFIRNYLKHINF"
     gene            39511..39699
                     /locus_tag="VBE22_000552"
     CDS             39511..39699
                     /locus_tag="VBE22_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEO7202026.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTVLIDGKP
                     KKVWVSARALKSGKVERV"
     gene            39831..40196
                     /locus_tag="VBE22_000553"
     CDS             39831..40196
                     /locus_tag="VBE22_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7202027.1"
                     /translation="MTVKINTRDGQIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQALLRKENYAKGVVVRSSEAGIAVDVYTVMSYGVKISEVSKNIQERVKFNLENQLG
                     LSADMVNVYVQSIKVVGED"
     gene            40196..41860
                     /locus_tag="VBE22_000554"
     CDS             40196..41860
                     /locus_tag="VBE22_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEO7202028.1"
                     /translation="MSKITTSLFQEMVQAASTRLSKQAEYVNSLNVFPVPDGDTGTNM
                     GMTMENGAKEVSDKPATTVGEVGQILSKGVLMGARGNSGVITSQLFRGFGQSIKDKEE
                     LTGQDLAHAFQNGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     KGAKRALAKTPEMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFKATPATM
                     TEMVNAEHHKAVVGHVATEDIKYGYCTEVMVGLKQGPTYVKEFNYEEFQGYLSNLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVENMRNQHDAQMQKVEVEETVK
                     ETKEYGIIAVVAGDGLAEIFKSQGVDYIISGGQTMNPSTEDIVKAIEKVNARNVIILP
                     NNKNIFMAAQSAADVVDIPAAVVETRTVPQGFTSLLAFDPAKSLETNVADMTNSLSDV
                     ISGSVTLAVRDTTIDGLEIHENDILGMVDGKILVSTPDMEKALKDTFDKMIDEDSEIV
                     TIYVGEDGKQALAETLSEYLEETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            42008..42892
                     /locus_tag="VBE22_000555"
     CDS             42008..42892
                     /locus_tag="VBE22_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEO7202029.1"
                     /translation="MSLIILTVILVLVIVLLITSLYVVKQQTVAIIERFGKYQKTATS
                     GIHIRVPLGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNIATQYRVNENNVTDAYY
                     KLIKPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIVKT
                     LITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVGIA
                     QQRKAIVDGLADSIQELKDANVTLTEEQIMSILLTNQYLDTLNTFAINGNQTIFLPNN
                     PEGVEDIRTQVLSALKTR"
     gene            complement(42984..43100)
                     /locus_tag="VBE22_000556"
     CDS             complement(42984..43100)
                     /locus_tag="VBE22_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEO7202030.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
BASE COUNT        14160 a         6860 c         8725 g        13377 t
ORIGIN      
        1 tgttggctgt gtgctggcac attgagaaac tttatatgga ataaactttc tggaataaat
       61 gagacattga catcagatat tgatgttgta ttttttgata aaaatatttc ttatgaagaa
      121 acggtggtat tagaacaaca attaaaggat aactatccac agtacgattg ggaacttaaa
      181 aatgaatttt acatgaatac tcattctcct aataccccaa agtatactag ctctaaagat
      241 gctatttcga aatttcctga aaagtgtact gcagtgggag caaggttaga tgatagaaat
      301 caactcgaac tttacctccc ttatggtgaa gaggaaatat taaatttcat tgttagtcct
      361 acgccatatt ttgaagagga tctattacgt tataatgttt atttgaagcg agtggataag
      421 aagaaatgga ataatatttg gcctcgttta acaatcataa aaaaatgata acgttactgg
      481 agttggctta acagctttta aatttttaga ctgacaaata gtatagacct ttaataaggt
      541 cgtttatcga taatcatagt gtactgtgtt ataatcgtta tagcataaaa acacgttgca
      601 gttggtagtc tgcaagcgtt cagtgaacgt cagtaacctt ccctccaggt cgtccattgc
      661 aatttaatat ttaaaggagg gggctgtatg aaaatgacag tctattttga tggtaatttt
      721 tggttgggtt tgattgagta cgatgatgat ggtgattaca aagtttttcg gtattttttt
      781 ggaaaagaac ctaaagatga tgatgttttt aattttataa atcataaact taatgatttg
      841 ataaaaaaat atgagtttgt taaaacggat atttctctaa agcgtacgaa tgaacacaaa
      901 aaaagtccta aaagaatgca aagagaaatt aatagagaaa aacgtaagcc agtagtatct
      961 acaaaagcgc aattggcgat gaaaactatt catatgagta taaaaaacga acgtcaatta
     1021 agtcagaaat gtaagaaaaa tgagttaaga aagcatcggt accaattaaa acaagaaaaa
     1081 cgttaccaaa agaaaaaagg acattgattg tgtcatcata taaaaagctt gagagtgtca
     1141 tctcaagctt tttatatgtt aactaatatg agtcactaaa tagtctgata atgctcaatt
     1201 aatgtgtttg gggaaaaaat catttcctta aaggacacca ttgtcatgtt gttatcacct
     1261 tactttttct atacttagta tatgtttaag aaaggaataa gttattttga gagattttga
     1321 ggagttgttt gataaagtaa agccaatcgt aatgaagctg agaagaaatt attttgttca
     1381 actttgggaa tatgatgatt ggattcaaga agggcgtatt gtacttttta ggttgcttga
     1441 agaacatccg tatttattgg ataatgaaag caaactattc atttacttta aaacaaaatt
     1501 ttcaaattat ttaaacgatg tattaagaca tcaagattgt caaaagcgac aatttaataa
     1561 aatgccttat gaagaaatca gtgaagttag tcactatgtt aagagtaaag gactagtttt
     1621 agatgactat attgcttatc gtgatacttt aacaaaagtg gaagaaactt tgtcagatat
     1681 tgataaagaa aaatttgaaa aactgattag tggtgaacgt ttcgcgggga agaaacaatt
     1741 catacgagat attcaacctt ttttcaatgc ttttaaagcg gattaatcga ttttatttct
     1801 gcagtagata gctgcagaat ttttttatag ggaaagttcg ctgatatatt cctgtgaaag
     1861 cctttgtttt ttgttataat ataataaaat gcttgtgagg tgtcagtatg agtaaagttc
     1921 gtttatatat cgctcgtcat ggtaaaacaa tgtttaatac aattggtcgc gctcaggggt
     1981 ggagtgatac tcctttaact acttttggag aactcgggat taaagagctt ggtttgggac
     2041 taaaggcttc aaatattagt tttaaagagg ctttttctag tgatagtgga cgtactttac
     2101 aaactatgga aattattctc agggaagtgc aacaagaaaa tattccctat acgagagata
     2161 aacgaatccg cgagtggtgc tttggtagtt tggatggagg atatgatggc gaccttttta
     2221 atggcgttct gcctcgagtt tcaaacggag atatgagcca tttgacacac gaagaaattg
     2281 ctaatttgat ttgtcaagtt gatacggctg gctgggcaga accgtgggca atattgagta
     2341 accgtattct gtcaggcttc actgctatag ctaaaaaaat agaggatatt gggggaggta
     2401 atgcgattgt tgtcagtcat gggatgacta ttgcgacttt tttatggtta atcgaccata
     2461 gtacccctcg tagtttaggc atagataatg gtagtgtctc agtcgttgat tttgaagatg
     2521 gaacattttc tattcaatct attggggata tgtcctatcg agaaaaagga agagaaattt
     2581 tagaaaaaac attgcaatga aaaaaaataa aattatccga ttcagtttag ttggtgttct
     2641 acttgcgata ctatgcttta gtctttttgc tttattgaag cctaacagtc aacaatcatc
     2701 atctcaaaag ttgaggaatg aggatataaa aaagatatcc tctcaaaaaa gaaataagaa
     2761 attacaatta ccagctgtat catcaaaaga ttggaacttg attttggtca atcgtgacca
     2821 taaacatgaa gaattaagtc cagatgtggt tcctgttgaa aatatttatt tggataaacg
     2881 tattacgaag caagctactc agtttttaga ggctgctaga gcaattgatt cacgagaaca
     2941 tttaatttcg ggttatcgta gtgttgccta tcaggagaag ttgttcaatt cttatgttac
     3001 tcaagagatg actagtaacc ctaatttgac gaggggacaa gcagaaaagt tggtaaaaac
     3061 ttactctcag cctgcaggtg ctagtgaaca ccagactgga ttagcgatgg atatgagtac
     3121 tgtagattct ttgaatgaga gcgatcctag agtagtcagt cagttgaaaa agatagctcc
     3181 acaatatggt tttgtcttac ggtttccgga tggtaaaaca gcagaaacag gggtaggtta
     3241 tgaagattgg cattaccgct atgttggggt agagtctgca aaatatatgg ccaaacatca
     3301 tttaacatta gaagaataca taactttatt aaaggagaat aaccaatgag gaaacgtttt
     3361 tccttgctaa attttattgt tgttactttt attttctttt tctttattct ttttccgctt
     3421 ttaaaccata agggaaaagt agatgctaat tctaggcaga gtgttaccta caccaaagaa
     3481 gaatttatac aaaaaattgt gccagatgcg caagatctag gaaagtcgta cggtattcgt
     3541 ccttcattta ttattgcaca ggcggctttg gattctgatt tcggagagaa aatattagct
     3601 aataagtatc ataatctgtt tggtttgctt gcagaaccag gaacgccctc aattacctta
     3661 aatgatagta gtacaggaaa aaaacaggaa aagcaattta ctcattataa atcttggaag
     3721 tattcaatgt atgattacct tgctcatata aaatctggag cgacaggcaa aaaagattca
     3781 tatactataa tggtgtctgt taaaaatcca aaaactttag tgcaaaaatt acaagatagt
     3841 ggttttgata atgacaaaaa gtacgctaaa aaaatgacgg aaatcattga tttgtatgat
     3901 ttaacaagat atgataagtg atattagcac tctttgaata agagtgctaa tttttgatgt
     3961 tttttccttg actatctttt aaaaaggagt ataataaaat catagttagc agtcgatgtg
     4021 taagagtgct aatttcagaa agaccgagag gggtgatgtt tttgtgatta cacaacgtca
     4081 aaatgatatt ttgaatttaa tcgttgagtt atttacacaa actcatgaac cagtagggtc
     4141 taaagcttta cagagaacga ttgaatctag tagcgccact attcgtaatg atatggcaaa
     4201 actagagaag ttggggttat tggaaaaagc acacacttct agtggacgga tgccgagtcc
     4261 agctggtttt aagtattttg tagagcactc ccttagacta gatagtattg atgagcaaga
     4321 tatttatcat gttataaaag cttttgactt tgaagcattc aaattagaag acatgcttca
     4381 aaaagctagt catattttat cggagatgac gggctataca tccgttattt tagatgtgga
     4441 gccagctcgt caacgtttga cagggtttga tgttgttcag cttagcaacc atgatgctct
     4501 agcagtcatg actttagatg aatctaagcc agtgacagtg cagtttgcta ttccaagaaa
     4561 ctttttgaca cgtgacttga tagcatttaa agctatcgta gaggagcgct tgctggatgg
     4621 tagtgtcatg gatattcatt ataagttgag gacagaaatt cctcagatcg ttcagaagta
     4681 ttttgtaact acggataatg ttttacaact atttgattat gttttttcag agctgtttct
     4741 ggaaacggta tttgtagctg gcaaggtaaa ttctttaaca tattcagatc tatcaacgta
     4801 tcagtttttg gataatgagc agcaagtagc aatttctttg aggcaaggtc tgaaagaggg
     4861 tgagatggct tctgtccaag tggcagatag tcaagaagcc gcacttgcag atgtgtcagt
     4921 tttaactcac aaatttttga ttccttaccg tggatttgga ctcctgagtt tgattggtcc
     4981 tattgatatg gattatcgta ggtctgttag tttggtaaac attatcggaa aggtattggc
     5041 tgctaaatta ggtgattatt atcgatacct aaattctaac cattatgaag ttcactgata
     5101 atggcagtat tcaataagtt atttaaaagg aggcactcag tgtcagaaga aattaaaaaa
     5161 gacgatttac aggaagaagt ggaagccact gaaactgaag agacagttga ggaagttatt
     5221 gaagaaattc ctgaaaaatc agagttagaa ctcgcaaacg agcgtgctga tgagtttgaa
     5281 aataaatact tgcgtgctca tgcagaaatg cagaatatac agcgtcgctc tagtgaagaa
     5341 cgtcaacaac ttcaacgcta tcgttctcag gatctggcaa aagcaatctt accatcgctt
     5401 gacaatctag aacgtgcctt ggctgttgaa gggttgacag atgatgttaa aaaaggttta
     5461 gaaatgactc gtgatagcct tattcaagca ctaaaggaag aaggcgtaga agaagtagaa
     5521 gtcgactcat ttgaccacaa ctttcatatg gcagttcaga cccttccagc cgatgatgaa
     5581 catccagccg atagcattgc agaagtcttt caaaagggat ataaattgca cgagcgtctg
     5641 ctaagaccag cgatggtagt tgtctataac taaggtaaat gagttttcgt ttttgtccgt
     5701 aatgacagta aactagatag caagttagaa gctattcagc ttgctgatta aactatagtg
     5761 attgcttaga attggaagta aaataattcg agtgcttact aagataaatt gaaataaaaa
     5821 gtaataaagt attataaaat aagaggtatt aacatgtcta aaattattgg tattgactta
     5881 ggtacaacaa actcagcagt agcagttctt gaagggactg aatcaaaaat cattgctaac
     5941 ccagaaggca atcgtacaac tccttcagta gtatcattca aaaatggtga aattatcgtg
     6001 ggtgatgctg caaaacgtca agcggtaaca aatccagata ctgttatctc tatcaaatca
     6061 aagatgggaa cttctgaaaa agtttctgca aatggtaaag aatatactcc tcaagaaatt
     6121 tcagcaatga ttcttcaata ccttaaaggt tatgctgaag actatcttgg agaaaaagta
     6181 gaaaaagcag ttattactgt tccagcttac ttcaacgatg cacaacgtca ggcaactaaa
     6241 gacgctggta aaattgcagg tcttgaagta gaacgtatcg ttaacgaacc aacagcagcc
     6301 gcacttgctt atggtatgga caagactgac aaggatgaaa aaatcttagt ttttgacctt
     6361 ggtggtggta catttgacgt atcaatcctt gaattaggtg atggtgtctt cgacgttctt
     6421 gcaacagcag gtgataacaa acttggtggt gacgactttg accagaaaat tattgatttc
     6481 ttggtagaag aattcaagaa agaaaatggt attgatcttt ctcaagacaa aatggctctt
     6541 caacgcttga aagatgctgc tgaaaaagct aaaaaagacc tttcaggtgt aactcaaact
     6601 caaatttcat taccgttcat cactgctggt tctgctggtc ctcttcactt ggagatgagc
     6661 ttatcacgtg ctaaatttga cgatctcact cgtgaccttg ttgaacgtac gaaaactcca
     6721 gttcgtcaag ctctttcaga tgcaggcttg tcattgtcag aaattgatga agttatcctc
     6781 gttggtggat caacacgtat cccagcagtt gttgaagctg taaaagctga aactggtaaa
     6841 gaaccaaata aatctgttaa ccctgatgaa gtggttgcca tgggtgctgc tatccaaggt
     6901 ggtgttatca ctggggatgt gaaagacgtt gtacttcttg acgtaacacc attgtcactt
     6961 ggtattgaaa caatgggtgg tgtcttcact aaattgatcg accgcaacac aactatccca
     7021 acatctaaat cacaagtctt ctcaacagca gcagacaacc aaccagccgt tgatatccat
     7081 gttcttcaag gtgaacgccc aatggcagca gataacaaaa cactcggtcg cttccaattg
     7141 actgatatcc cagctgcacc tcgtggaatc ccacaaattg aagtaacatt tgatatcgat
     7201 aaaaatggta ttgtatctgt taaagctaaa gatctcggta ctcaaaaaga acaacacatt
     7261 gttatccaat ctaattcagg attaactgat gaagaaattg ataaaatgat gaaagatgct
     7321 gaagcaaatg ctgaagcaga tgcaaaacgt aaagaagaag ttgatcttaa aaatgaagtt
     7381 gaccaagcca tctttgcaac agaaaaaact attaaagaaa ctgaaggcaa aggttttgat
     7441 acagaacgcg atgcagcgca atcagcactt gatgagttga aaaaagctca agaatcaggt
     7501 aaccttgacg acatgaaagc taaacttgaa gctcttaacg aaaaagcaca agctcttgca
     7561 gttaaacttt acgaacaagc ggctgcagca caacaagcag ctcaaggggc tgaaggtgca
     7621 caatcagctg attcatcaag caagggtgat gatgttgtag atggcgaatt cactgagaaa
     7681 taattattaa tattgttcag attcatttga atataagcat gaaaactata ctagcatagt
     7741 aaagttcttc gtgataggga ttgctcaata atctagataa gtttcagatt acataagcta
     7801 atttcgctat cactaaataa aaacatatta ataataaata ggcggggcgc ctcgctccgt
     7861 ctgttttatt aagtgtcata tatatgttaa ctatttagag ctgtaactgg gcaagaataa
     7921 ttgttaatct cttcaagtgt agtatatgaa caaaatataa aggattagat aatgaacaat
     7981 acagaatttt atgatcgtct tggcgtttca aaagatgctt ctcaggacga aataaaaaaa
     8041 gcttaccgta ggatgtctaa gaaataccat ccagatatta ataaggaaac gggagcagag
     8101 gaaaaatata aagaggttca agaagcttat gaaaccttga gtgatacaca aaaacgtgct
     8161 gcctatgatc aatatggcgc tgctggggca aatggcggtt ttggtggctt cgatggtggc
     8221 ggttttggtg gcttcgatgg tggtggcttt ggtggttttg aagacatctt ctcaagcttt
     8281 tttggtggag gagggatgcg caatcctaat gcaccacgtc aaggagatga cctacaatat
     8341 cgtgttaatt taagttttga agaagctatc tttggagctg aaaaagaagt atcttataat
     8401 cgggagtcat catgtcatac ttgttcagga agtggagcta agccaggtac tagccctgtg
     8461 acttgtcaaa agtgtcatgg ttcgggtgtg attaatgttg atacacaaac acctctagga
     8521 acaatgcgtc gccaagtgac ttgtgatgtt tgtcagggta gtggtcaaga aatcaaagag
     8581 aaatgtccaa catgtcatgg aactggtcat gagaagaaga cgcataaagt atcagttaaa
     8641 atacctgcag gagttgaaac tgggcaacaa attcgtttaa caggacaagg tgaagctggc
     8701 ttcaatggtg gtccttatgg tgatctcttt gttattatta acgttctccc aagccaacag
     8761 tttgagcgta atggatcaac aatttattat actttgaaaa ttagctttgt ccaagctgct
     8821 ttgggtgata caattgatat acctactgtc catggagcgg tagaaatgtc aattcctgct
     8881 ggtactcaga caggaaaaac gttccgtctt cgcggaaaag gtgcccctaa acttcgaggg
     8941 ggtggacaag gtgatcagca tgttactgtt aacatcgtaa ctcctactaa attgaatgat
     9001 gctcaaaaag aggcattgca cgcttttgca gaggcaagtg gagataagat ggtacatccg
     9061 aagaagaaag gtttctttga taaagtcaaa gatgctttag atgttgatta aaaaataaaa
     9121 gtctagaatc gaaagtattc tagacttttt tgtagttatt gctagaggga ttgcttgaac
     9181 ctaaatttta cagtacaatg aatctaatat tgtaggggga caatttttat ggtaaccaaa
     9241 gttgaagaga ttcgctcata tttgatagct tctatacaaa atggtaagtt ggctccagga
     9301 gatcgcctac catctataag acagttagct aatcaatttt cctgtaacaa ggatacagtg
     9361 caacgagttt tgatggaatt gcgttttgat aattatgtct atgcaaagcc taggtcaggc
     9421 tattacgtct ttgattctca tgaagaggaa gttgaagaag gggttagttt accaaactct
     9481 gagattgcaa atatagctta tgatgatttt agattgtgtt tgaatgagac ccttattggt
     9541 agggaagatt accttttcaa ttattactat cgtcaagaag gtcttcttga tttaagtaaa
     9601 gcggtggcta aattaatgga agaaacaggg gtctatgttc ctcttgatga tattgttatt
     9661 acagctggta ctcaacaggc attatttatt ttgacacagg ttacctttcc aaatcgaaaa
     9721 tctcgagttt taatagaaga accgacctat cctcgtatga ttgaactaat caaaacacaa
     9781 aatttaccct atgaaactat ctctcgaggt actcatggaa ttgattttca gcgtttagag
     9841 gagattttcc agacacaatc aattaagttt ttttatgtta tacctcgcat gcataatcct
     9901 ttgggaacat cctataatcc ggtagagatg aaaagattaa tagagatggc agagaagtat
     9961 gatgtttata ttgtggaaga tgactatatg tctgattttg caagtcagtc accattacat
    10021 tattatgata ctcacgggcg tgttatttat ctaaaatctt tttcaaaggc tattttccct
    10081 gctttaagat tagctgcgat ttgtttacca caagctttaa aatcaacatt tatggcttac
    10141 aagaagttga tggattatga tactaatctg attttacaaa aagcattagc gctttatatt
    10201 gaaaatggcc tttatggtaa gaatagtcaa tatttgaaat atcgttatca gaaagacctt
    10261 gcaaattcaa aatctatttt agctgatcag cctaatctac ccccatatag tttacatcac
    10321 gatagtgtat tatttgattg ttcgaaactc gataacttca aaatattacg gcaatacggc
    10381 gatactttgg aaaattattt ttgtcaaaaa tcgcatcaat ctctcttaca agtaaaaaat
    10441 gattcctgct taaagcagtt cttgggatcg ttgtagtata attgtagggg gacagtttaa
    10501 aatagatatt atagttttca taaatgaaag gtggtaaaat aaacccatga caaggtataa
    10561 ggcacagatt tcttatgatg gttcagcttt ttcaggcttc caaagacaac caaattgtag
    10621 gacagttcaa gaagaaattg agagaactct taagcgccta aatagcggga acgatgtgat
    10681 aatccatggt gctggtcgca cagatgctgg tgttcatgct tatggacaag ttattcattt
    10741 tgatttacca caggcgcgtg atgtagaaaa attacgcttt ggtttagata cacagtgtcc
    10801 agacgatatt gatattgtca aagtagagca agtgtctgat gattttcatt gtcgatatga
    10861 taaacacatt aaaacttatg agtttttagt agatattggt aggccgaaaa atcctatgat
    10921 gagaaactat gcaacacatt acccttatcc tgtcattatt gagcttatgc aagaagctat
    10981 taaagattta gtaggtaccc atgattttac aggctttact gcttcaggta cgagtgtaga
    11041 aaataaagta cgtactattt ttgatgcgaa aattcagttt gaagcgtcta aaaatctttt
    11101 aatttttacc tttacaggaa atggtttttt gtataaacag gtgcgtaaca tggttggtac
    11161 cctgctaaaa attggtaatg gacgcatgcc tattagccaa atcaaaacaa ttttacaggc
    11221 taaaaatcgg gatttagcag gtccaactgc ggcaggaaat ggtttatatt tgaaggagat
    11281 tatttatgaa gacaaggaat gttttagcaa tttcaggaaa tgatattttc tctggcggcg
    11341 gcttgcatgc agatttagca acttacgttg ttaataaatt acatggtttt gtggcagtaa
    11401 catgcttaac agcaatgtca gataaagggt ttgaagtcat tcctatagag gcatctatac
    11461 taaagcagca actagaaagt ttaaaagatg tagagtttgg gtctattaaa ctaggattgc
    11521 ttcccaatgt agaaacagca caagttgttt tggaatttgt aaaatcaaaa caagaatgtc
    11581 cagtagtctt agacccagtc ttagtttgta aagaaaatca tgatttagaa gttagccaat
    11641 tacgtgagca gctaattgcg tttttcccat atgctgatgt gattactcct aatttagtag
    11701 aggctcaatt attaacgggt ctttcgattg aaaacttaga tcaaatgaaa attgctgctg
    11761 agaaacttta cgatatgggg gctaaacatg ttgttatcaa agggggcaac cgtttaaatg
    11821 ctgaagaggc tacagacctt tactatgatg gtgaacgttt tgaaacctat gtttttcctg
    11881 tagtagatgc caataataca ggtgctggat gtacatttgc gtcaagtatt gccagccaat
    11941 tagctatggg taaaaatgta gaagatgcag tcaaaatgtc taaaggtttc gtctatcaag
    12001 ctattaaagc atcggataag tacggagttg tccaacattt ttaggagatt ttaatgagga
    12061 aagagaaaac tagtcaattg gttcaattgg caattgtaac agctatatct attgtattgg
    12121 ggatgtttat aagtattccg acaccaacag gttttttgac attactagat gctggaatat
    12181 tctttgctgc cttttacttt ggcaaaaaag aaggggctgt ggtaggtgcc ttagctggtt
    12241 ttttaattga tttattgaaa ggttatccca attggatgtt ctttagttta ttgattcatg
    12301 gggcgcaagg gtatttagcg ggtttaccag gtagaagacg tcttttaggt cttattagtg
    12361 caactcttgt tatggtatta ggttatgcta tagctagtgg attgatgtat ggttggggag
    12421 ctgttctacc agatattcct ggcaatatta tgcaaaatat ggttggtatg gtcgttggtt
    12481 ttgctcttaa taagtctcta gaaagagtga agaaatgata ctagattata ataagttaaa
    12541 gcaggaaact aaagctattg tagttgatat tattgaacgt agcgctctta aaaaaggtca
    12601 aatttttgta ctaggtctat catctagtga ggtttctggt ggtttaattg gaaaaaattc
    12661 tagttctgaa attggtgaaa ttatcgttga agttatcctc aaagagttac attcacgcgg
    12721 catctattta gcagttcaag gttgtgaaca tgttaataga gcgttggttg ttgaagcaga
    12781 attggctgaa cgtcaacagt tggaagtcgt taatgttgtt cctaatctac atgctggcgg
    12841 tagtggtcag gttgcagctt ttaagttgat gacatcacca gttgaggttg aagaaattgt
    12901 ggcacatgct ggaattgata ttggagatac ttctattgga atgcatatca agagagtaca
    12961 agttcccttg attccaatta gtcgtgagtt gggaggagca cacgttacag cactagctag
    13021 tcgtcctaaa ttaattggcg gtgctagagc aggatatacg agcgatccaa tccgtaaatt
    13081 ttaaaataaa cctctgctct gcagaggttt attatttatc tcaacgagag tggttattgg
    13141 agaaattgta cgttggtaag tcaatacctt cttcaagaat agctttttga taaagtttgt
    13201 aaaattcagc atagatgtca aactgagcac cgttttgcgt gaagatatta atacgataaa
    13261 caaattgacc attagcattg gttgtaggac caaaaacagt aggctctcca acgatagcag
    13321 ggtgttttga aacatactct tcattgattt ttgttacaat atcagaaatc tgatcaaggt
    13381 ttgtatgaac aaataaagga atatcaattt gagcacgcat attgctacga gatttattgc
    13441 tgacaattgt aatattacga ttaggaatga agtgtaaggt tccatcgaaa tcatgaattt
    13501 gtgtggtgcg tatcccaaca ttagtaacag tgcctgatac agtaccaaca ttaataatat
    13561 caccaacatc aaattggttt tctagtagaa taaaaaagcc attgacaaca tctgataaga
    13621 agccttgagc accaagtcca atagcaaccc cagcaatacc agccccagct aataaactag
    13681 aaattggaac gcccaataaa cttaaaatcc aatagaatag aaagaaataa agggtatagt
    13741 ttaaaacatt gtgagaaagt ttagctaatg ttttttgacg agctactttt tgtctagaaa
    13801 aggcgagtga gcgattaaca gtcttttcga aaagataatt gatgacttgc ctaacgataa
    13861 cgaatgcaat gatcaataat aagattgata ttagtttggt gaagaaagta aataaaactt
    13921 cctctacatt aagatggtct ataaattttt ctaaagtaat catagtaata gtctagtaaa
    13981 aaaaaagcca tagagcaagt attttaaaag cttatcgtta agcagtttaa agcttctttt
    14041 ttgcataaaa actttgtaaa acgttttaaa ttatggtaaa ctaaactgta tctaaataat
    14101 tttaaggaga aatgacttaa tgtctacatc atttgaaaat aaagctacaa accgtggtat
    14161 tatcacattt acaattagtc aagatgagat caagccagct cttgatcaag cattcaacaa
    14221 agttaaaaaa gatttgaacg ttccaggatt ccgtaaagga catatgcctc gtacagtttt
    14281 caaccaaaaa tttggtgaag aagcactcta cgaaaatgca ttgaatttag tgttgccaaa
    14341 ggcttacgaa gcagctgttg cagaacttgg tttagacgtt gttgctcaac caaaaattga
    14401 tgttgtatca atggaaaaag gtcaagattg gaagttgaca gctgaagttg ttacaaaacc
    14461 agaagttaaa cttggagatt acaaagacct ttcagtagaa gttgatgctt caaaagaagt
    14521 ttcagctgaa gaagttgatg ctaaagttga acgtgagcgt aacaaccttg cagaattgac
    14581 tgttaaagat ggtgaagcag cacaaggtga tacagttgtt attgactttg taggttcagt
    14641 tgatggtgtt gaatttgatg gtggtaaagg agataacttc tcacttgaac ttggttcagg
    14701 tcaatttatc ccaggatttg aagaacaatt agtaggttct aaagctggtc aaactgtaga
    14761 cgttaatgta acattcccag aagattatca agcagaagat cttgcaggta aagatgctaa
    14821 atttgttaca acaattcatg aagtaaaaac taaagaagtt ccagcacttg atgatgaact
    14881 tgcaaaagat attgatgatg aagttgaaac acttgatgag ttgaaagcaa aatatcgcaa
    14941 agagctagaa tcagcaaaag aaattgcatt tgatgatgca gttgaaggtg cagcaatcga
    15001 attagctgtt gccaatgcag aaattgttga attaccagaa gaaatggttc atgatgaagt
    15061 acatcgtgct atgaatgagt tcatgggtaa tatgcaacgt caaggaattt caccagaaat
    15121 gtacttccaa ttaacaggta caactgaaga agaccttcat aagcaatacc aagcagatgc
    15181 tgacaaacgc gttaaaacta accttgttat tgaagcaatt gcagctgcag aaggctttga
    15241 agcaacagat gaagaaatcg aaaaagaaat cacagatctt gcttcagaat acaatatgga
    15301 agctgaccaa gtacgcggtc tactttcagc agatatgctt aaacatgata ttgcaatgaa
    15361 aaaagcggtt gacgttatca ctagctcagc tactgtaaaa taattgctta ttaaattttg
    15421 aaaagattgg gcttcccagt ctttttattt gtgattattc ttttgacgaa tagctaaaaa
    15481 tagcgtaaaa tagtaaatag cgaaaatttg aaaatatgac ttatctattt tctatctata
    15541 aagaatattg ctaaacaaaa tgatagtctt acacaattaa ggagaataac tttggaatta
    15601 gaagtatttg ctgggcaaga aaaaagtgaa ctttcgatga ttgaagttgc tcgcgctatt
    15661 ttagagcaac gtggtcgtga taacgaaatg tacttcagcg atttggtaaa tgatattcaa
    15721 acctaccttg gaaaatctga ctcagcaatt cgtgagtcat tgccattttt ctattcagat
    15781 ttgaatacag acggaagttt tattccactt ggcgaaaaca aatggggact tcgttcatgg
    15841 tatgctatcg atgaaattga cgaggaaatc atcactcttg aagaagatga agatggcgct
    15901 ccaaaacgta agaagaaacg tgtcaatgct ttcatggatg gtgatgaaga tgctattgat
    15961 tacaatgatg atgatccaga agatgaagat ttcacagaag aaacaccaag tttagaatat
    16021 gatgaagaaa accctgacga tgaaaaatca gaggtagaat cgtatgattc tgagattaat
    16081 gaaatcattc ctgatgaaga tttagatgag gatgtagaaa tcaacgaaga agacgatgaa
    16141 gaggaagaag aggaagaaga ggtttagaca gggattttgt atttgacaaa tagtcagata
    16201 tagtttaaac tattattcgg gcacctctta tgaggtcaga taaagctccc tagtgatagg
    16261 gggctatttt gtttttggga aagatacaga atggttccag atagttttct gagtaggccc
    16321 ttgatcagat agtttctact agttttgact agagaagctg gtattattta tcaatcatgt
    16381 gttttgggtt ttacccgtaa actaactgtt agattaacat ttaatttaag gagtttttaa
    16441 tgacaaagta tatttttgtt acaggtggtg tggtctcttc tattggtaag gggattgttg
    16501 cagcaagtct tgggcgctta ctaaaaaacc gtgggctaaa ggtgacaatt caaaaatttg
    16561 atccatatat taatattgac ccagggacaa tgagccctta tcaacatgga gaagtttatg
    16621 ttacagatga tggtgcagaa actgaccttg accttggaca ctacgagcgt tttattgata
    16681 ttaacttaaa caaatattca aatgttacaa cgggtaagat ttatagtgaa gttcttaaaa
    16741 aagaacgccg tggagaatac cttggtgcaa ctgttcaagt tatcccacac gttacggatg
    16801 ctttgaaaga aaaaatcaaa cgtgctgcga caactacaga ttcagatgtt attattactg
    16861 aagttggcgg tactgttgga gatattgaga gccttccgtt tttagaagca cttcgtcaaa
    16921 tgaaggcaga tgttggttct gataatgtta tgtacattca tacaacgtta ttaccatacc
    16981 taaaggctgc cggcgaaatg aaaacaaaac cgactcaaca ttctgttaaa gagttacgtg
    17041 gcctaggtat ccaacctaat atgcttgtta tccgtacaga gcaaccagca ggtcaaagta
    17101 ttaagaataa attggcacaa ttttgtgatg ttgcaccaga ggctgttata gagtcccttg
    17161 atgttgacca tatttatcaa attccactta acatgcaagc tcaaaatatg gatcaaattg
    17221 tttgtgatca cttgaagctt gagacacctg ctgctgatat gacagaatgg tcagcaatgg
    17281 ttgataaagt aatgaacctt gagaaaaaag ttaaaattgc attggttggt aaatatgttg
    17341 aattacctga tgcctacctt tcagttgtgg aagcccttaa gcactcaggg tacgtcaacg
    17401 atgttgctat tgatttaaaa tgggtaaatg ctgctgaagt gacagaagat aatattaaag
    17461 aacttgtcgg agatgcagat ggtattatcg ttcctggtgg atttggccaa agaggtagtg
    17521 agggtaaaat tgaagcaatt cgctatgcac gtgaaaatga tgttccaatg cttggtgttt
    17581 gtctcggaat gcagctaact tgtgtcgaat ttgcgcgtaa tgttttaaaa cttcatggag
    17641 ctaattcagc agagcttgac cctaaaacac cgttcccaat cattgatatt atgcgtgatc
    17701 aaatcgatat tgaagatatg ggtggtactc ttcgccttgg tctatatcca tgtaaattga
    17761 aagcaggttc aagagctgcg gcagcttata ataatcaaga agttgtccaa cgtcgtcacc
    17821 gtcaccgtta tgaatttaat acaaaattcc gtgagcaatt tgaggctgct ggatttgtct
    17881 tttcaggggt atcaccggat aatcgtttga tggaagttgt tgagttacca gagaaaaaat
    17941 tctttgttgc agcacaatac catccagaat tacaatcacg tcctaaccat gctgaagagc
    18001 tttacacagc ttttgtaaca gcagcagttg aaaatatgaa ataactttat cgcctcccag
    18061 tattaggagg cgattttatt tttttagata gtttcactat caaagtatca ttagctcttt
    18121 tctttttcta aaaataaggt ataataggtt gcatgaaaaa gattcgatta tcaaagttta
    18181 ttaaaatgat tgttgttatt ttgtttttaa ttagtgtagc agctagtttt tattttttcc
    18241 acgttgccca agttcgagat gataaatcct ttatttcaaa tggtcaacgt aagcctggaa
    18301 actctttata tgcttatgat aaatcctttg ataagctatt aaagcaaaaa atagaaatga
    18361 caaaccaaaa tataaagcaa gttgcttggt atgttcctgc tgctaagaaa actcataaga
    18421 cagctgttgt cgttcatggt tttgcgaata gcaaagagaa tatgaaggca tatggttggc
    18481 tgtttcataa gttaggatac aatgttctta tgcctgacaa tattgcacat ggtgaaagtc
    18541 atgggcagtt gataggctat ggctggaacg accgcgagaa cattatcaaa tggacagaaa
    18601 tgatagttga taagaatcca tcaagccaaa ttactttatt tggtgtttca atgggtggag
    18661 caacagtcat gatggctagt ggtgaaaaat tacctagtca ggttgttaat atcattgaag
    18721 attgcggtta ttctagtgtt tgggatgaat taaaatttca ggctaaagag atgtatggtt
    18781 taccagcctt cccactctta tatgaagttt caacaatttc taaaatcaga gcaggttttt
    18841 cgtatggaca agcaagtagt gtcgaacaat tgaaaaagaa taatttacca gccctcttta
    18901 ttcatggtga taaggataat tttgttccaa caagtatggt ttatgacaac tataaagcta
    18961 cagcaggtaa gaaagagctt tatattgtaa aaggggcaaa acatgcgaaa tcttttgaaa
    19021 cagagccaga aaaatatgag aaacgtatct ctagtttttt gaaaaaatat gaaaaataat
    19081 attgacaaca atatgataag ttgatataat ttattatgtt gttaagcggg gatggcggaa
    19141 ttggcagacg cgcaagacta aggatcttgt gaccgctttt ggtcgtgagg gttcaagtcc
    19201 ctctctccgc agactagtcg agttcatttg gaactcgact ttttgcatat ttaagataaa
    19261 gatggagtat cacatgtcaa aagtacgtgg ctttgaatta gttagccaat ttagcaataa
    19321 agagttatta ccaaaacgag aaacagctca cgcagctggc tatgatttaa aagtagctaa
    19381 aaaaacggtt attgaacctg gtgaaattac attggtcccg accggtatta aagcacacat
    19441 gcaaccaggg gaggtcctgt acctttatga tcgttcatcg aatccacgta aaaaagggat
    19501 tgtcctaata aattcagttg gtgttattga tggtgactac tataataacc aagtcaatga
    19561 aggacatatt tttgcacaga tgcaaaatat tactgatcag gctgttattt tagaagaagg
    19621 ggaacggatt gttcaggctg tttttgcccc attcttattg gcagatgacg atcaggctac
    19681 aggaatgcgt acaggtggtt ttggtagtac tggaaaataa aattatttta cgaataatgt
    19741 tagaaaaggc tagacttact tcttagcctt ttttgatgag agtaaactta tattttctct
    19801 aacttggtgt gatataatga gtgtactagt tatttggtac caagctatca tattaattta
    19861 gaagttataa aggaggcctg agtggctaag aaaaaatcag tcttcacctg tcaagagtgt
    19921 ggttaccaat cacctaagta tttgggacgt tgtccaaatt gctcagcctg gtcttcattt
    19981 gtagaagagg ttgaagttca agaagttaaa aatgcacgag ttagtttgaa cggtgaaaag
    20041 tcccgaccga cgaagttaaa agatgtttcc tcaattaact attctagaac taagactgat
    20101 atggatgaat ttaaccgtgt tctaggaggc ggcgttgtac caggaagttt ggttctgatt
    20161 ggaggagacc ctgggatagg taaatctact ttattactac aagtatcaac tcagctagct
    20221 aataaaggta cagtattata tgtctcaggt gaggaatctg cagaacagat taagttacga
    20281 agtgagcgtc taggcgatat cgataatgaa ttttaccttt atgccgagac taatatgcaa
    20341 agtattcggt cggagattga gaagattaaa ccagattttc ttattattga ttctatacaa
    20401 actattatga gtcccgaagt atcaagtgtc caaggctctg ttagccaagt tcgcgaagtg
    20461 accgctgagt tgatgcagtt agctaaaact aataatattg ctacttttat tgtaggtcat
    20521 gtgactaagg agggaacatt agcaggccct cgaatgctag aacatatggt ggatacggtt
    20581 ctttattttg aaggggagcg tcatcacacg ttccgtattt tacgggcagt caaaaaccgt
    20641 tttggttcga ccaatgaaat aggtatcttt gaaatgcagt cgggtggtct agttgaagtt
    20701 ttgaatccaa gtcaagtttt tcttgaagaa cgtttagacg gtgcaacagg atctgctatt
    20761 gttgttacta tggaaggaac aaggcctatt ttagcagagg ttcaagcttt agtgactcca
    20821 actgtttttg gaaatgctaa gcgcacaacc acaggtcttg actttaatcg tgtcagtctt
    20881 attatggcag ttctagaaaa acgttgtggt ttattgcttc aaaatcaaga tgcctattta
    20941 aaatcagcgg gtggtgttaa attggatgaa ccagcaattg atttagctgt tgctgtagct
    21001 attgcttcca gttacaaaga aaaacccaca aatccaaaag aatcatttat tggagaaatt
    21061 ggactcactg gtgagattcg tcgcgtgaca cgtattgagc aacgtattaa tgaggctagt
    21121 aaattgggct ttactaaaat ttacgcacca aagaattctc tagctgggat tgaaatacct
    21181 aaaggcattg atgttattgg tgtgacaact gttagtcagg tcttaaaagc agtgttttca
    21241 acataatgat tagattcggt tcagccgaat cttttttggt ggcgagagac atttcgtgaa
    21301 aaataatggg caaaatgata atataaaagg gttggtattt agtaaatatc gaatgatgtt
    21361 acactaacaa aggagatctc tatgaccact tattttgata actttttaaa aaccaatcaa
    21421 gcctatgctg acttacatgg aacagctcat ctacctatca agccaaagac aaaggttgct
    21481 attgtaacct gtatggattc tcgtttacac gttgctcaag ctctagggct agatttagga
    21541 gatgctcata ttttacgtaa tgctggtgga cgagttacag acgatgttct acgttcttta
    21601 gttatttcgc aacaacaatt aggaacacga gaaatcgttg ttctacacca tacagattgt
    21661 ggagcacaga cgtttactaa cgaagctttt gctactcaat tacagagaga tttgggtgtt
    21721 gatatgcatg gacatgattt tcttcctttt aatgatattg aagaaagcgt tcgtgaggat
    21781 gtggcaaaat tacatgcttc tccattaatc ccaaatgatg ttgttatttc tggtgctatt
    21841 tatgatgtag atactggtcg tatggtggaa gttaaataga aaaaatcctg tccttagcga
    21901 tgggtttttt tattttagaa aactagattt aaaggtaaca gttaggctta tcatgcctta
    21961 tgctggaatt cagacagagg aaacatcttt tgctatactg aaactatatt ataaaagcgg
    22021 aggaaattta aaatgaaaaa atatgatgtc attgttcttg gttttggtaa agcaggtaaa
    22081 acattagcag caaaattagc aacacaaggt aaaagcgttg caatggtcga agaagacgat
    22141 aaaatgtatg ggggaacatg tatcaacatt ggatgtattc cgacaaaaac gcttttagta
    22201 tcagctagca aaaatcacga ttttcaagaa gctatgacta ctagaaatga agttacaagt
    22261 cgtttacgtg ctaaaaattt tgctatgttg gataataaag acactgtgga tgtttataat
    22321 gctaaagctc gctttatatc aaataaagtg gtcgaattaa caggaggagc tgacaaacaa
    22381 gaacttacag ctgatgttat tattatcaat actggtgcta agtcagtaca attaccaatc
    22441 ccaggattag ctgattctca acatgtttat gattcaacgg caatccaaga attggctcat
    22501 cttcctaaac ggttaggtat cattggaggc ggtaatattg gtcttgaatt tgcgacttta
    22561 tattctgaat tagggagcaa agtgacagtt attgatagtc aatctcgtat ctttgcgcgt
    22621 gaggaagagg aattatcaga gatggctcaa gattatttag aagagatggg aatatctttt
    22681 aaattatcag ccgatatcaa atctgtgcaa aatgaagatg aagacgttgt tattagcttt
    22741 gaggatgaaa aattaagctt tgatgcagta ttatatgcaa caggacgtaa gccaaataca
    22801 gaaggtttag cacttgaaaa tacagatatt aaattgacag aacgtggagc tattgctgtt
    22861 gatgaatatt gccaaacttc tgttgagaat atttttgcag ttggtgatgt taatggcggc
    22921 cctcaattca catatatttc tttagatgac tcacgtattg tcttaaatta tttgaatggt
    22981 gataaggatt attctctaaa aaatcgagga gcagtaccga cttcaacctt tacaaatcct
    23041 cctttagcaa cggtaggtct agatgaaaag accgctaagg aaaaaggata tcaagttaaa
    23101 tctaatagcc ttcttgtttc tgccatgcca cgtgcacatg ttaataatga ccttagaggt
    23161 atttttaaag ttgtggtaga tactgagaca aatcttattt tgggagctcg cttatttggt
    23221 gcagaatccc atgaattaat taatatcatc acaatggcaa tggataataa aatcccatat
    23281 acttatttcc aaaaacaaat tttcactcac ccaacaatgg tagagaattt taatgatttg
    23341 tttaactttt aagaatattt ttaaaaagta aggcaatttt gctttacttt ttttatcgaa
    23401 aattagtgtg gctattatta ctgattatca tttttagtga tttagtggta taatagggtt
    23461 gattggcaat catctgtaag agtttgaatg atgttttgcc cccttgacta attcagttaa
    23521 actgaaaaag tttactatca gaaaatatag gagtagaatt ttggctaata agattcgtgt
    23581 gcgttatgca ccaagtccaa caggattgtt acacatcgga aatgcgcgta ctgcgctctt
    23641 taactacctt tatgctcgcc atcatggtgg ggattttgtt attcgtattg aagatactga
    23701 ccgaaaacgc cacgtcgaag atggtgaacg ctctcaattg gagaacttac gttggctagg
    23761 tatggactgg gatgaaagcc ctgaaacaca tgaaaattat cgtcaatcag aacgccttga
    23821 gctctaccaa cgttatattg atcagttgtt agcagaaggt aaagcctaca agtcctatgt
    23881 gactgaggaa gaattagcag cagagcgtga acgccaagaa ttggcaggcg aaacacctcg
    23941 ctatatcaac gaatttatcg gaatgtcaga aactgaaaaa gaagcttaca ttgcagaacg
    24001 tgaagcatca ggcatcattc cgacagtacg ccttgctgtt aatgaatctg ggatttataa
    24061 atggactgat atggtaaaag gtgacattga gtttgaaggc tctaatatcg gcggagactg
    24121 ggttattcag aagaaagatg gttatccaac ttacaatttc gcagtagtta ttgatgatca
    24181 tgacatgcaa atttcgcatg ttattcgtgg cgatgaccat attgcaaata cgccaaaaca
    24241 attaatggtt tatgaagcac tcggttggga agcgccacaa tttggtcaca tgacattgat
    24301 tataaactca gagactggta agaaattatc aaaacgcgac accaatactc ttcaatttat
    24361 cgaagactat cgtaaaaaag gttatatgtc ggaagctgtc tttaatttca ttgcccttct
    24421 tggttggaat cctggtggag aagaagaaat cttctcacgc gagcaattaa ttaatctatt
    24481 tgatgaaaat cgtcttagca aatcaccagc agcattcgat cagaagaaga tggattggat
    24541 gagtaatgat tacctaaaaa atgctgattt tgagagtgtt tttgcacttt gcaaaccatt
    24601 cttagaagaa gctggtcgac ttacagacaa agctgaaaaa ttagttgagc tttataaacc
    24661 tcaattaaaa tcagctgatg agattgtacc attaacagac cttttctttg cagattttcc
    24721 agaattaaca gaagccgaaa aagaagtaat ggcagcagaa acagtcccaa cagtcttatc
    24781 tgcttttaag gaaaagttag tatcgctttc tgacgaagaa ttcactagag atactatttt
    24841 ccctcaaatt aaggctgttc aaaaagaaac aggtattaaa ggtaagaacc tatttatgcc
    24901 tattcgtatt gcggtttctg gtgaaatgca tggaccagag ttaccagata ctatttattt
    24961 attaggtaaa gaaaaatcag ttcaacatat cgataatatg ttagcaaaat tataatataa
    25021 aaagtcgtga attttcacga ctttttgtgt acttttacca attgtatttt tcaacattgt
    25081 ctttggtaac aaggtaaatt ggagaaattg tttctttctc tacttttttg cctttataat
    25141 gatcaattgc tgcttgaatt gctatttctc ccatcttagc tggttgttgt gcaatagtgg
    25201 ctgaaatatc tcctttttta atggcatcat gggcatctgg ctgaccatca attcctacga
    25261 taagaacatt ttgcaagcca gcagatttga cagcctgtgc agccccaagt gccatttcat
    25321 cattttgtgc gaaaataatt tggacatctt tatgtccttg aatcatattt tgtgtagtat
    25381 tcaaagcttt ggcgcggtca aaattggcag attggcttga aagaatgtca agtttagatt
    25441 ttgcaactga atgaaatcct ttaccacggt ctacagtagc agaggcacct ggtactccag
    25501 ataattcaaa ggcttttgct tttttgccta gttttttaac agcataatcc gcagccatct
    25561 taccagcagc aacgttatct gaagcgacag tagttaatac tttgcctccc tcactaccac
    25621 ggtccattag aataacagga atattagcgt tgtttgcaga cttaatagct gttacaatag
    25681 cttttgagtc aacgggatta atcaaaatag catctacgtt ttgactaata aagttttgga
    25741 catcatctgc ttgacgtgcg gcgtcatctt gagcatcagc taccttaatg cttatttttt
    25801 tattgctagc atatttgtca ataccgtctt tcatcgcaac aaagtaggga ttgttagttg
    25861 ttgaaataga tacacctagt ttcaagtttt tagcagattt tttggttaca ttttttgtag
    25921 aattcccagt agaatttcca agtccagttt taccacatgc acctagaatg aggacaatag
    25981 acatcaataa ggctagaaag ccaagttttt ttccaaattt catttgaaac tcctttagat
    26041 aaaatataat gaatttatag aaattagagt aaagactatt ttgctacttt aaagcgatca
    26101 agtaaaacag ccattaaaat aacgatacct ttcacaactt gttgccaaaa tgcagagaca
    26161 ccaatgatgt ttaatccatt gttcaggaca ccaataatga gagcaccaat caatgttcca
    26221 atgatacgtc ctttcccacc agataaagac gttccaccta agacaacggc ggcaatagca
    26281 tccatctcat aacttgctcc agctgttggt tgtgctgagc taaggcgtga tgtgataatt
    26341 aacccagaaa tggaagccat gatacctgag atagtgtaga tgataatttt aactttgttt
    26401 aacttaatac ccgagatata cgctgctttt tcattacctc ctaaagcgta aacagatttc
    26461 ccgaaggctg ttttatgtaa caaaatatac aaaataataa aagtaagaaa catgagaatg
    26521 actgggaatg ggataccaac aatatagcct tgtcccaaaa attggaatag aaaactatcg
    26581 gataagccag cggtaattgg gttgccgttt gaataaacta gcgttgctcc tctaaagata
    26641 gtcattgttg ccagtgtaac gataaatggt gctaattttc cgtaagaaat gaagagtccg
    26701 tttagcattc ccaagatgcc acctaaggca acagataaaa cgatagcaat accaacgggt
    26761 actccgtggc cgattaatcc agctgttaat gcactagata gggctaatat ggacccgact
    26821 gataaatcaa taccgccagt caaaataacg aaggtcatcc cgaaagcgat aaaaccattt
    26881 gcagttactt gtaataataa attgagtaga ttattggttg tgaggaaatt aggattaatg
    26941 agtgtgataa taaccattaa accagctaac gcaatcaggg ttgttaactc tgatatatat
    27001 ttcatcatat ttttcacttt acttacctcc tgtggctagt tgcataactt tttcttggtc
    27061 agcttctttt cgactgagtt ctccactgat tcgaccttcg tgcattacca tgatgcggtc
    27121 actaacacct agaatttcag gtaagtctga tgataccatg acaattggaa cacctctgtc
    27181 tgctagttca ttcattaatt ggtaaatctc tcgtttagct ccgacatcaa caccacgtgt
    27241 tggctcatca agaattaaga ctttaggagc aataccaatc catttagcga gaacgacctt
    27301 ttgttggtta cctccagaaa gatttccaac ctctaaatct ggtctccctg atttaatgta
    27361 gaggcgatta atcaattgtt gcacaaatgt tgtgcttgtt ttttcatcaa agaaaccatg
    27421 tttgctaaaa tctttagtgc tagggagagt catattgtcc ttgatattga agtctaggat
    27481 gagaccttca tcttttctat tttcggtcaa aaaaccaatg ccctgcttga tagcttgact
    27541 tgggctagta atagtgattt cttggttatc aattttcacc ttaccagact tctttttatc
    27601 aattccaaag atagttcgca taacttcagt acgcccagct cccattaatc cagaaaatcc
    27661 aagaatctcg cctttacgga cataaaagga aacgtcctcg aaattatctc cacagaggtt
    27721 actaacttca aaagcaacag gcccaatttc agaatgtttt tcaggatagt aatcttccaa
    27781 ttttcggcca accatttttt taaccaattc gtcagaattt gtgagtgatg tttcttttgt
    27841 atctacaatg acaccatctc tcatgacggt tacgaagtca gtgattttaa aaatttcctc
    27901 cattcgatgg gagatataga caatgcccac tccttcttgt tttaaacttc ggataactcg
    27961 gaaaagattt tcagtttcac gatctgttaa agcagcagtc ggttcatcca ttaccaaaat
    28021 agagagttgg tttaggagac ttttggcgat ttcaatcatt tgttgttgtc caacagacaa
    28081 gttaccaatc gggatgtcta ggggaatagt cacacctaat cttttaaaag cttcaagtgc
    28141 tttttgtctc attaattttt gattgagtaa gccaaaagtt gtcttgattt cacgtccaag
    28201 aaagagattt tccaagacag tcatttccgg ccaagtattc atctcttggt gaataaaact
    28261 aatgccaaat tcctctgctt cttgagggtt tgaaaaagtt cgttcctcgc catcaatata
    28321 aatagttcct gtacttgctg gaaaaagtcc tgttagaatg ttcattaagg ttgatttacc
    28381 cgcaccattt tcacccataa gcgcgtgtat ttgaccagat tgtaattcca aatcaatctt
    28441 ctctagaact ttattagtac cgaaggattt ggagatattc ctcatgtcaa ttttcatgga
    28501 ctaaactcct ttctagatga taacaccaga ttgtagaata atatttgaat acggtgtatt
    28561 ttcacctgta cgaataactg ctttgacatc ttgggtcatt tgttttaaat gatcgtgact
    28621 gacgtactct atgctaacag agttatcaag tttagctaat aaccttgata attgatccgg
    28681 atttgcttcc ttgatttctt ctgctaaaat gactttttca actaagatat tctctaaata
    28741 gatatcaagt acctcttgga aactaggtat tccagaggtc aaagacaaat caatctttgg
    28801 aatgccattt ggaacaggta gacccagatc tccaatacaa actcgatcag tatgcccaag
    28861 gtcatctgct aatttagcga gatgactatt taatatgcca gtctttttca taatgttggt
    28921 tgtcctccat ttctttaatt gtaggcatgc caccctgtgc accaaatttt tgcacggata
    28981 agtgagcagc tagagttgca aaacgtaagg cattagcaat cttaaatttt ttcgagatag
    29041 cataaccgaa tgcaccgtta aaagtatcac ctgcaccagt tgtatcaaca gctttagttt
    29101 ctaaggcagg aattaaatgg ctttcttttc catcagagta gatggctcct tttgtgccga
    29161 gtgttacaat tagtttttca ggatatgtgg ctaatatatc ctctagtttt tggtttggga
    29221 acagctcttg acattcgtgt tcatttggtg tgaaataatc gaccttatcg agcatctcaa
    29281 tatcagtctt tctagatggt gcaggattat agagtaattt aatactatgt tctttacaaa
    29341 aattagcaat tttcatattt gcttgatgag gaatttcatt ttgtaataca actaaatcag
    29401 cttctttaat tattgaccac tcctgagacc actttttagt atcaactttt ccgttggctc
    29461 caggacagta aatgattcga ttatcattat tataaagagt gatttgggca acacctgaag
    29521 aagatggtat cgttcccaca aaatccgtag taacatggtt tttgtttaaa ttatccaaaa
    29581 gaatgggacc aaaagaatcc tcgccaatgg caccaagaat agtaatatta tctcgatctt
    29641 ggctaagacg cccaatagcg acagcctgat tagcaccttt acctcctgga accattgaaa
    29701 aacgttggcc aaatacggtt tctccctctt tggcgatacg attggtttcc ataactaagt
    29761 ccatggaaat actaccgata atgacaatgt tactcatcta ttttcctcct tagggtttca
    29821 cgttcgacat aatgaacggg taatttaata cgcttattgg taataggtaa ttgattagta
    29881 attttgaaaa ttaattcagc tgctttttgt cccataatat atgatgattg gtgaatagtt
    29941 gacaacgaag ggtagataaa ttggctcatt aaaatatcat cataaccaat aatttgtaca
    30001 tcttcaggga ttcgcttccc tcgattaaga atctcgtgta ggtaggcaat agcatgtata
    30061 tcagatggag cgataatgct atcaatatct ggatggtggt caagtaaagt tttcgcttca
    30121 atttgaatct ctgcaaaatc aaaactggca ctatcttcga tagcaaaacg agcccctttg
    30181 tttaataggt aattttggct tccttgaaac ctttgattaa ggttatcagc tttatctagc
    30241 ggaccacgaa tgagtaatat attggtagca cctgctgtcc aaatagcttg agctgctaat
    30301 tttcctccct ctttattatc agagaaaacg ccgtactgtg tttcttgatt aacgcggtca
    30361 acaactacaa ctggaatatc tatttcagga tgatttttgg tgaaatcgtg tgttgtaata
    30421 atccctgcag cattactttg taatagcaca tttaagtact cttcttctaa atggctcttg
    30481 ttatttgtgt tgccaagcat gacacgataa ccctgttctt ttagaaattc ttcaactcct
    30541 ctagcaagtc ttggaaagaa agggtttgaa atatcgggaa gtagtaatcc aacaagttga
    30601 ttttttttgg ttttcagaga ttgtgcaagg aaatttggaa cataatggag ttcgcggata
    30661 gcttgctcga ttttttgact tgctttttga ctaacgtaac cattttggga tatatacctt
    30721 gagacagtag atgttgatac gccagctttc tcggcgactt gtctaatcgt actcatagaa
    30781 actcctttct tttattgtgg gaacggtttc ataattagaa ttgtaatcgc ttactaaaaa
    30841 attgtcaagc tattttttaa ataatttaat gataaaatat cattattttt agaggtcata
    30901 ttcaagtcat aaagctgtga tactatgttc acatcaaaag atcaaaggag aaaaaccatg
    30961 tcacatgctt ggaaagaatt aaccttttat aagaaaaaat acctcctgat tgaattgcta
    31021 atcattgtta tgatgttcat ggtagttttt ttatcaggtt tagcaaacgg tttaggtaga
    31081 gctgttagtg ctgctattga aaataaccca gctcaaacct atattttgaa tgaaggtgct
    31141 gaacaagtga ttacatcatc tgtattgaca actaaagatc agacagattt aaacagtttg
    31201 aatttaaaag atagtaccac tttaaatatt caacgttcga gtttaactcg acaaggacat
    31261 gaaaagaaaa ttgatatttc ttattttgct attgataaag attcttttat ggcacctaca
    31321 ctttctgagg gaaaacaact gacaagttac aaaaaagcta ttattttaaa tgatagttta
    31381 aaagctgaag ggataaagtt aggtgataaa gtgattgata agtcatcatc tatatcacta
    31441 acagtcgttg gatttgtcca taatagtatg tatggtcacg gacctgtggc atttattgat
    31501 aaagacattt ataccgaaat taataaaaag attaatcctc aataccagtt cttacctcag
    31561 gcattagtta tgaaaaatga taaaagtata tctcatttac caactcaatt agaagctgtt
    31621 agtaaaaaag atgtcattca acatattcct ggttatagtg ccgaacaatc cactttgaat
    31681 atgattttat gggtccttgt cgtagcttca gctggtattt taggtgtctt tttctacatc
    31741 atcacacttc aaaagcgtca tgaatttagc gtcatgaaag ctattgggac aaaaatgtca
    31801 gagattgccc tatttcaatt gagtcaagtc atcatattgg cactgtttgg tatcattgtt
    31861 ggagatggat tggcgattgc tctctcttat gtattaccag ctcaaatgcc atttgttatt
    31921 aattggcaaa atattatttt agtatcgttt gtatttttag tcattgcaat gattagtagt
    31981 gctctgtcaa ttgtaaaagt tgctaaaatc gacccagtcg aagttattaa tgggggagga
    32041 gaataatgtc agcaatctta gaattaaaac acatctcaaa acactatcca gatggagatg
    32101 agcttttatc aatattagat aatttagatt tatcagtttc tgcaggtgag tttgtagcta
    32161 tactgggacc ctcaggttca ggtaaaagca cacttttatc cattgcaggg ttacttcttg
    32221 gcgcagatca aggtagtttg tatgtcaatc atgagaacgt tacggatttg tctcaaagac
    32281 aaagaacaca attgcgccgt gaagcactag gttttatatt tcaaagtcat caactgcttc
    32341 cttatttaac aatccaataa cagttgcaac aagaggctcg atttgcaaaa cactatgata
    32401 agaaaacaag cttggaagaa attaataaat tattatcaga tttaggaata gagcagtgtg
    32461 cacataaata ccctaaccaa ttatcgggag gacaaaagca acgcgcagca attgctagag
    32521 catttattaa tcatcctaag gttatcttgg cagatgagcc aacagctagc cttgatgaag
    32581 aacgtgggcg tcaagttaca gaacttattc gtcaggaagt taaatcacat aatactgcag
    32641 ctattatggt gacacacgat gagcgcgttc tagatttagt agataccgtt tatcgtttaa
    32701 aggacgggaa attagttaaa gaaaacttct aacttgtgtt acaataagct agaggtgatt
    32761 taatgattaa aatattagtt gtagaggata acattgttca acaaaaaatc attaccacaa
    32821 aattaacaca agagggctac caatttatca ctgcttcaaa tggacaagaa gctctaaatt
    32881 gtctagatac agaagaagta caattgatta taactgatat catgatgccc atgatggatg
    32941 gttatcaatt aattcaagaa ctcaggtcag ctgcttataa tgtcccaatc attgttatga
    33001 cagcaaaatc acaaatggaa gatatgacaa aagggtttgg ccttggtgca gatgattata
    33061 tggttaagcc tgtgcagtta caagaattag ctttacgtat taaggcactc ttgaggagag
    33121 ctaatattgt agctcaacat cagctaatta ttgggaatac ttgtcttaat gaagatgaat
    33181 taagcttgaa atattttgag caagaaattg tatttcctca gaaagaattt agggttcttt
    33241 tccacttact tagctatcct aataggattt tcacacgttt agaactattg gatagtattt
    33301 ggggaatgga tactgattta gatgagcggg tggtagatgc ttgcatcaat aaaattagac
    33361 gaaaagtaga acacttacca gattttaaga ttgagacggt gagaggggta gggtatcgtg
    33421 caaaaaatga ctaagctaag gcgatttcgg tttccgttaa gattttattt cacattaatg
    33481 tttgtgttaa cgatgctttt ctcagtccta gcttctcttt tactagttgc agcaatagtt
    33541 tttacttttt ttcaaggagt cttaactact catgtattac aagttagcgc tctcgctgtt
    33601 gtttttttaa gccttgtgat agctagtatt agtatgtggt acggttctta tcatttaaca
    33661 aaaccaattt tagatattag ccgtattgtg tccaatgttg cagacggtga ctttgaaggg
    33721 catatttatc gaaacagtaa tcgtcgtaaa tcctatgagt attataatga attggatgag
    33781 ctatctgaaa gtattaatca gatgattgtt tctttatctc atatggatca tatgcgaaaa
    33841 gactttatca ctaatgtttc tcatgagcta aagactccga ttgctgcagt agctaatatt
    33901 gtagaactat tacaagatcc agaattagat gaagaaacac aaagtgagtt attaggtctt
    33961 gttaaaacag agagcctaag gttaacaaga ttatgtgata cgatgttgca aatgtcgcgc
    34021 gtggataatc aggagactat tggggagcta tcctctgttc gtgtagatga acaaattcgt
    34081 caagcaatga ttagtttaac agaacgttgg caagctaaaa gaataaattt tcaactggat
    34141 tcaaaaccat atactgttta ctcaaactct gacttactga tgcaagtttg gataaacctt
    34201 ttagataatg caataaaata ttccgatgat attgtagact tacgtgtacg aatggaagaa
    34261 actaataatc attatctgag agttattatt tctgataaag gtaggggaat ttctcaatat
    34321 gatgttcagc atatatttga taaattttac caagcggatc agtcgcataa tcaacaagga
    34381 aatggattag gactagcaat agttaaacgt ataattgttc tttgtaaggg acgtattagt
    34441 gtctccagtc agtttgaaat tggaacagaa ttttgtgtag aactaccttt atcataatta
    34501 tttaaaacaa taactgccaa ttggcagtta ttgttttatt tttttagaaa taaatatact
    34561 aaaaatagac aaaaactata aaaatatttg acaataaata tatcaagtgt ataatattac
    34621 aaaacatagg aatttggaag gggatttttt atgggaaaag aaaaattaat tttagcttat
    34681 tctggtggtt tagatacctc tgttgctata gcttggttaa aaaaagatta tgacgttatt
    34741 gctgtctgta tggatgttgg cgaaggtaaa gatcttgatt ttattcacga taaggcttta
    34801 acaataggtg ccatagaatc ttatatttta gatgttaaag atgagttcgc tgagcatttt
    34861 gtgttaccag ctttacaggc acatgcgatg tatgaacaaa aatacccgct tgtttcagcg
    34921 cttagtcgtc caattattgc tcaaaaatta gttgaaatgg cacatcaaac aggtgcgacc
    34981 acaattgccc atggatgtac aggtaaagga aatgaccaag tgcgttttga agttgcaatt
    35041 gcagtactag atcctgaatt aaaagtaatt gcccctgttc gtgagtggaa gtggcatcgt
    35101 gaagaggaaa ttacttttgc aaaagctaac ggcgtaccta ttccagcaga tttagataac
    35161 ccttattcta tagaccaaaa tttatgggga cgtgccaatg aatgtggtgt gcttgagaat
    35221 ccttggaatc aagcgccaga agaagctttt ggtattacaa aatcaccaga agaagctcct
    35281 gattgtgcag aatatattga tattacgttc caaaatggga agcctatagc tataaacaat
    35341 caagaaatga cactagcaga tttgatttta tcgctaaatg aaattgcagg aaaacatggt
    35401 attggacgca ttgatcatgt tgaaaatcgt ttagtcggta tcaaatcacg tgaaatttat
    35461 gaatgtcctg cggcaatggt actgttagct gctcataaag aaattgaaga tttaacatta
    35521 gtccgtgaag tgtctcattt taaacctatc cttgaaaatg aactatcaaa tcttatttat
    35581 aatgctttat ggtttagtcc agctacaaaa gctatcattg cctatgtcaa agaaacacaa
    35641 aaagtggtta atggtactac aaaagttaag ttatataaag gttctgccaa agtcgttgct
    35701 cgccactcgt ctaattcatt atatgatgag aacttagcaa cttatacagc tgctgataac
    35761 tttgatcaag atgcagcagt tggttttatc aaactctggg gacttccaac ccaagttaat
    35821 gcacaagtaa acaaaggata aataaaggaa gataagagga tgacaaaaaa tcataagtta
    35881 tggggtggtc gttttgaaag tagtcttgaa aaatgggttg aagagttcgg tgcatcaatc
    35941 agtttcgacc aaaagttagc tccctatgat atgaaggctt ctatggccca tgtcacaatg
    36001 ttaggcaaaa cggatattat tagtcaagaa gaagcaggct tgataaaaga tggtttaaaa
    36061 atactgcaag ataaatacag agcaggtcaa ttgactttta gcatatcaaa tgaagacatt
    36121 catatgaaca tagagagctt attaaccgct gaaattggag aagtagcagg taagcttcat
    36181 actgcacgtt cacgaaatga tcaagtggct acagatatgc acctttattt gaaagataaa
    36241 ttgcaagaaa tgttgaagaa attgcttcat ttacgaacaa ccttagttaa tttagcagaa
    36301 aatcatatct atactgttat gccaggatat acacatttac aacatgctca gccgatttca
    36361 tttggacatc atctaatggc ttactataat atgtttacgc gtgatacaga gcgattagag
    36421 tttaatatga aacacaccga tctatcacca ttaggagcag cagctctagc agggaccacg
    36481 tttcctattg accgccatat gacaactcgt ttattagatt ttgaaaaacc atatagtaat
    36541 tcgcttgatg ctgtctcaga tcgtgatttt atcatagaat ttttatccaa tgctagcatt
    36601 ttaatgatgc atttatcacg tttttgtgaa gaaatcatta attggtgcag ttatgaatat
    36661 caattcatca cattatcaga tactttctct acaggatcat ctattatgcc tcaaaagaaa
    36721 aatcctgata tggcagaatt aattcgaggg aagacaggcc gtgtttatgg aaatttattt
    36781 agtttactaa ctgttatgaa atcattgccg ttagcttata acaaagattt acaagaagat
    36841 aaagaaggga tgtttgatag cgtagaaaca gtcagcatag ctattgaaat catggctaat
    36901 atgcttgaga caatgactgt gaatgagcac ataatgatga cctcaacgga gactgatttt
    36961 tcaaatgcaa cagaattagc agattattta gcaagtaagg ggataccttt cagaaaagca
    37021 catgaaattg taggtaaatt ggttttagaa tgcagtaaaa atggttctta tttacaggat
    37081 atacctttaa aatattatca agaaatttca gagttgatag aaaacgatat ctatgagata
    37141 ttaactgcaa agacagcagt taaacgaaga aattcattag gaggaacagg gtttgaccag
    37201 gttaaagaac agatcctgtt ggcacgtaaa gaattgaaag cggaatgact gctttctgaa
    37261 aaaaacctcg tttaactctg ttaagtttct cacgtatttt cagtaaaaaa ctagcatttt
    37321 ttatagaatt gtgctagaat taacatgtaa gtgggcaacg cccaaaaaat aaataggagg
    37381 cctgaataat ggcaatcgtt tcagcagaaa aattcgtcca agcagctcgt gataacggct
    37441 atgcagtagg tggatttaac acaaacaacc ttgagtggac tcaagctatc ttgcgcgcag
    37501 cagaagctaa aaaagctcca gtacttatcc aaacttcaat gggtgcggct aaatacatgg
    37561 gtggttacaa actttgtaaa caacttattg aaactttggt tgaatcaatg ggtattactg
    37621 taccagttgc tatccacctt gaccacggtc attacgatga tgcccttgag tgtattgaag
    37681 taggttacac ttcaattatg tttgatggtt cacaccttcc agttgaagaa aaccttgaaa
    37741 aagcacgcga agttgttgct aaagcacatg ctaaaggtat ttcagtagaa gctgaagttg
    37801 gtactatcgg tggtgaagaa gatggtattg tcggtaaagg tgaattagca ccaatcgaag
    37861 acgctaaagc tatggttgaa actggtattg acttcttggc tgcaggtatc ggtaacattc
    37921 atggaccata cccagcaaac tgggaaggac ttgaccttga tcacttgaag aaattgactg
    37981 aagctgttcc tggtttccca atcgtgttac acggtggttc aggtattcct gatgatcaaa
    38041 tccaagaagc tatcaaactt ggtgttgcta aagttaacgt taacacagaa tgtcaattag
    38101 ctttctgtca agcaactcgt gactacgctg ttgaattcaa cgctaacgaa gctgaataca
    38161 tgaagaaaaa actctttgac ccacgtaaat tcttgaaacc tggtttcgat gcaattacag
    38221 aagcagttga agaacgtatt gacgtgtttg gttcagcaaa caaagcataa ttaacataaa
    38281 acagataata gttaaaagct cacttatggt gagctttttt tggattatat gactttttat
    38341 taaatagata ctctttaaaa attgatatgc ttaagataat ttcgaataaa tgccgctgaa
    38401 tctgtgagtt gttgctcttc ttttgggctt agtgaaagcg aaattctttc tacaattcct
    38461 actctaccaa taatagctgg atatccaagg taaacccctt cggactcata gaaggtagat
    38521 acaggtagta actcatggga atcatttaac acagctaaaa cgatgcggac agctgcggta
    38581 gcgataccat aactagtata ttttttacca aaaaagacag tgtgcccgcc ttttaaagaa
    38641 gcttctttta tctgttctaa ttcttctgct tttaatagtg atatagctgg ttgtcctttt
    38701 acagttacct gactccaagc gacaaattgg gagttaccct gctctcctag attataacca
    38761 ccgatactcc ttggatcaac ttgtaggcgt tgaccaagca ctcttttcat ccgagcagta
    38821 tccaataagg ttcctgttcc gataacgcgt gatttaggaa attgagtata ttcttgataa
    38881 agctgggtga tggcatcaac aggattagta acgacaataa ggattcctct aaaaccagat
    38941 gattttaact tttggctaac gtcgtagact tctttactgg taaatggtag ttcagcaaag
    39001 cgattagctt ctttgttatt ctgtagtgaa atatcaccta aggctgaaat tacaacatca
    39061 gcatctgaca acgcgtcata gtcgtttaat actatattag cgtgataagt agcatttgcc
    39121 agagcatctt gaaaatcaat catatcagca ttaaccttat caatattagt atcaataaag
    39181 acataatcat caccaatacc ttgtgcaata agagcgtgtg caacagttga tccaacgtgc
    39241 cccatgccta taattccaat tttacgtgtc atatgaaacc tcctttgatt attgttgtct
    39301 attataatat tttgaaagaa aatgtaaact aaaaaatgaa tcagaaggaa gtgtgtctta
    39361 ttataaaaaa tatgtaagcg atttagataa aacaaatgaa aaagtaattt tgaaaagaac
    39421 ttgctagaag aagtaagttg tgatagaata gtctagtatg tggtgctagc acatccgtaa
    39481 atattgatca atataggagg aactcgtaaa atggctaaag tatgttattt cacaggacgt
    39541 aaaacagttt ctggtaacaa ccgttcacac gcgatgaacc aaacaaaacg tactgttaaa
    39601 ccaaaccttc aaaaagttac tgttcttatc gacggtaaac caaaaaaagt ttgggtttca
    39661 gctcgtgcgc ttaaatctgg taaagtagaa cgcgtttaat aaactgagaa agccttaggg
    39721 ctttttttag tgtttctaag gctgatttga tagttagatg cctctatttt tttacaataa
    39781 tagtgagata tgatagaata aagtgataaa aactttttga ggtagtagat atgactgtaa
    39841 aaatcaatac aagagatggt caaattgaac tctctgatga cgttattgca actgtcgttg
    39901 gaggttcagc aacggaaatt tttggcgttg ttggtatggc aagtaaaagt gctatcaaag
    39961 ataactttca agcacttttg cgtaaggaaa actacgcaaa aggtgttgtt gtgaggtcta
    40021 gtgaagcggg cattgcagta gatgtttata ctgtcatgag ttacggcgtc aaaattagtg
    40081 aagtttcaaa aaatattcaa gagcgagtga aattcaatct cgaaaatcag cttggtttat
    40141 ctgctgatat ggtgaatgtt tatgtgcaaa gtattaaggt tgtaggagaa gattagtgtc
    40201 aaagattacg acaagtttat tccaagaaat ggtacaagct gccagcacac gtctgagtaa
    40261 acaggcagag tatgtcaact cattaaatgt attcccagtt ccagatggtg atactggtac
    40321 gaatatgggg atgactatgg aaaacggagc caaggaagtt tcggacaaac cagcgacgac
    40381 tgttggagaa gttggtcaaa ttctttctaa aggtgtattg atgggagcac gtggaaattc
    40441 aggtgtaatt acatcacaat tattccgtgg ttttggtcaa agtatcaagg ataaagaaga
    40501 attaacgggt caagatttgg cacacgcttt ccagaatggt gttgaagtag cttataaagc
    40561 cgttatgaaa ccagttgaag gaactatctt gacagtatca cgcggtgcag ctaccgctgc
    40621 tctaaagaaa gctgaagaaa ctgatgatgc agttgaagtt atgcgcgcaa ctctcaaagg
    40681 tgctaagcgt gctcttgcta aaacaccgga gatgctacct gttcttaaag aggttggtgt
    40741 tgttgattct ggtggtcaag gactcgtctt tatttacgaa ggtttcctct cagcactgac
    40801 tggggaatat atcgcatcag aagattttaa agcaactccc gcaacaatga ctgaaatggt
    40861 taatgcagag caccataaag ctgtagtagg ccatgtagcc acagaagata ttaaatatgg
    40921 ctattgtaca gaagtcatgg ttggtttaaa gcaaggacca acttacgtta aggaatttaa
    40981 ctacgaagaa tttcaaggtt atcttagcaa tctaggggat tctttactag ttgttaatga
    41041 tgatgaaatc gtcaaggttc atgttcatac agaagatcca ggtcttgtta tgcaagaagg
    41101 tttgaaatat gggtcgcttg tgaaggttaa agttgaaaac atgcgtaacc aacatgatgc
    41161 ccaaatgcaa aaggttgaag ttgaagaaac tgtcaaagaa actaaagaat atggtattat
    41221 tgctgttgta gctggtgacg gtctagctga gatttttaaa tcacaaggtg ttgattatat
    41281 catctctggc ggccagacta tgaacccatc tacagaagat attgtaaaag cgattgaaaa
    41341 agtaaacgct agaaatgtta ttattttacc aaacaataag aacatcttca tggctgcaca
    41401 gtcagctgca gatgtcgttg atattccagc agcagtagta gagacacgta cagttccaca
    41461 aggatttact agcttacttg cttttgatcc tgctaagtct cttgaaacta atgtagctga
    41521 tatgacaaat agcctctcag atgtcattag tggtagtgtg actttagctg ttcgtgatac
    41581 aacgatagat ggccttgaaa ttcatgaaaa cgacatcctt gggatggttg atggtaaaat
    41641 tctagtatct acaccagata tggaaaaagc acttaaagac acttttgata aaatgattga
    41701 tgaagatagt gaaatcgtga caatctatgt tggtgaagat ggtaaacaag cattagccga
    41761 gaccttgtcg gaatatcttg aggagactta tgaagatgtt gaagttgaga tccaccaagg
    41821 agatcaacct gtatatccat acctcatgag cgttgaataa ttgataaaat aaaaaattag
    41881 gagagatact tctaattttt tattttatca gagtttctat tttagtatga taaagcatca
    41941 gtattttgaa aattttgaca aattgtgtta gaattgtttt aaaacattca tacaaaggag
    42001 atttatgatg tcattaatta ttcttacggt tattttggtt ttagtgattg ttctgttgat
    42061 aacttcttta tacgttgtta aacaacagac agtagccatc attgaacgct ttggtaaata
    42121 tcaaaagact gctactagtg gtattcatat tcgtgtacca ttgggtatag ataagattgc
    42181 agcacgagtg cagttacgtc ttttacagag tgaaattatc gttgagacaa aaacaaaaga
    42241 taacgttttt gtcactttaa atattgctac acaatatcgt gtcaatgaaa ataacgttac
    42301 agatgcttat tacaaattaa taaagccaga agctcagatt aaatcttata tcgaagatgc
    42361 tttgcgttca tcagtaccta aacttacatt agatgagcta ttcgaaaaga aagatgaaat
    42421 agccttggaa gttcaacatc aagtggcaga agaaatgtca acatacggct acatcattgt
    42481 taagacttta attactaagg ttgagccaga tgcagaagta aaacaatcca tgaacgaaat
    42541 caatgctgca caacgtaaac gtgtagcagc tcaagaatta gcgaatgctg ataagattaa
    42601 aattgtaact gctgcagaag cagaagcaga aaaagatcgt ctacatggtg tgggtattgc
    42661 gcaacaacgt aaagcaattg tagatgggtt agctgattcg attcaagaat tgaaagatgc
    42721 taacgtgaca ttgacagaag aacaaatcat gtctatcttg ttaacaaatc aatatttaga
    42781 tactttaaat acatttgcta ttaatggtaa tcaaacgatt ttcttaccaa ataacccaga
    42841 aggtgttgaa gatattcgta cacaagtttt atctgctctt aaaacaagat aatgaacttt
    42901 ttatagaagc aaaataacca gagcatttgc tctggttatt ttgtgtctga cttatttatt
    42961 ataattggag agacggaaaa aagttatttt ttaccgtaac gtttgtactc atgaatgatt
    43021 ttcagagcac gttttctagt tttaatgtta ggacttttaa gtctgcgata ggctgacgat
    43081 aggtctgttt tttcagccat gattacctcc tatagacaat tt
//
LOCUS       DATRTN010000010        38899 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.010, whole genome shotgun sequence.
ACCESSION   DATRTN010000010 DATRTN010000000
VERSION     DATRTN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38899
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.010"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..910)
                     /locus_tag="VBE22_000557"
     CDS             complement(<1..910)
                     /locus_tag="VBE22_000557"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO7202031.1"
                     /translation="MYLIEPIRNGKRITDGAIALAMQVYILQNVFLDDDILFPYYCDP
                     KVEIGKFQNAVIETNQEYLKEHDIPVVRRDTGGGAVYVDSGAVNICYLMKDHGQFGDF
                     KRAYEPAIKALKTLGASSVEMRGRNDLVIDGKKVSGAAMTIVNGRIYGGYSLLLDVDF
                     DAMEKVLNPNRKKIESKGIKSVRSRVGDIRSHLSEDYRHITTDQFKDLMVCQLLHIDH
                     IDQAKRYHLTEKDWAAIDALADEKYKNWDWNYGNSPQYSYHRDARFPSGTYDFHLEIE
                     KGIITNCRIYGDFFSSKDISDIENLLI"
     gene            complement(937..1815)
                     /locus_tag="VBE22_000558"
     CDS             complement(937..1815)
                     /locus_tag="VBE22_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEO7202032.1"
                     /translation="MTVWKTLEKTNHSQSEILSQLIEESDAIVVGIGAGMSAADGFTY
                     IGPRFEEAFPDFIAKYQLLDMLQASLYDFEDWEEYWAFQSRFVALNYLDQPVGQAYLD
                     LKDILAKKEYHIITTNADNAFAVTDYNLEKVFHIQGEYGLWQCSQHCHQQTYRNDQAI
                     RQMIAQQKDMKIPSNLIPKCPKCDQPFEINKRNEEKGMVEDADFHAQRQRYENFLSQH
                     QNDKVLYLEIGVGHTTPQFIKHPFWRFVSLNENSLFVTLNHKHYRIPQKIRSRSVQLT
                     QHIAELIAEAKKEIIG"
     gene            complement(1784..2602)
                     /locus_tag="VBE22_000559"
     CDS             complement(1784..2602)
                     /locus_tag="VBE22_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEO7202033.1"
                     /translation="MPNQKQLLLAMIEYLQSENLTDVDDLRTTDLQTVWRGLVNQQDP
                     QNISQEYLSLEDRYLSHWWNTQKVKTIDVCHQTVYSNVFTYHGDICLLQVDAIVNAAN
                     SKLLGCFIPNHHCIDNHIHTFAGSRLRLACHQLMTQQGRMEAVGQAKLTESYHLPCKY
                     VIHTVGPYVKVDQKPSRIREDLLKSSYKSCLQLAVRANLKTIVFPCISTGEFGFPNQR
                     AAELAVQAILEWQRENQHKLYIIFNTFTPKDQEIYQKLLLKEEIDDSVEDSRKN"
     gene            complement(2595..2927)
                     /locus_tag="VBE22_000560"
     CDS             complement(2595..2927)
                     /locus_tag="VBE22_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEO7202034.1"
                     /translation="MKKIANYLLIEKNEELYTISLTPELQDDLGTVGYVEFTDDTNLE
                     VDDVILNIEASKTVMAILSPLTGKVVKVNTAASQEPTLLNSEKADENWLVVLTDVDYA
                     AFEALENA"
     gene            complement(2956..3942)
                     /locus_tag="VBE22_000561"
     CDS             complement(2956..3942)
                     /locus_tag="VBE22_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEO7202035.1"
                     /translation="MKLSVLDYGLIDYGKTASDAIQETILLSQEAERLGYHQFWVAEH
                     HGVRAFSISNPELMIMHLANQTKSIKIGSGGIMPLHYSSFKLAETLKTLETCHPNRVS
                     IGLGNSLGTVKVSNALRSLHKAHDYEEVLEELKSWLIDESSSKKPLVQPTLSSFPDLY
                     VLGSGQKSAYLAAKLGLGFTFGVFPFMDKDPLTEAKKLSSLYYHQFEEYYPNKSPNLM
                     VAAFVVIADTSEEAENIAKTLDIWMLGNKDFNEFATFPTIEEANHYQLTPEQKAKIKS
                     NRHRMIVGDPKQVKESLDALVNASQAEELLLIPLVPGLDQRIKSLKLLSQLY"
     gene            complement(3939..5138)
                     /locus_tag="VBE22_000562"
     CDS             complement(3939..5138)
                     /locus_tag="VBE22_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEO7202036.1"
                     /translation="MNNVQGNLFRPLTLPNGLSLENRFVLSPMVTNSSTSEGFVTDDD
                     IAYAVRRAKSAPLQITGAAYITEYGQLFEYGFSVSKDEDIPGLTKLAKAMKSKGAKAV
                     LQLTHAGRFSSHTLARHGYVYGPSPMQLQSPYPHQVKELTHKDILRIIDEYVQATRRA
                     IQAGFDGVEISSAQRLLIQTFFSTFSNQRKDEYGPQTLTNRCRLGLEVFKAVQKVIRE
                     EAESDFILGFRATPEETRGSQIGYSIEEFMEFLEKILAIAQVDYLAVASWGHDVFRNT
                     IRSEGVYKGQLVNQVIFEHFGDRVPIMATGGINSASKVFEALQHAHMVGASTPLVVDP
                     EFLQKIKAKRSDQINLRIKVSDLEGLAIPKASFKDIVPLMDYGESLPKEAREVFRELR
                     SNYREER"
     gene            complement(5131..5967)
                     /locus_tag="VBE22_000563"
     CDS             complement(5131..5967)
                     /locus_tag="VBE22_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEO7202037.1"
                     /translation="MEWQDLAQLPVSIFKDYVTDAQDAEKPFIWTEVFLREINRSNQE
                     IILHIWPMTKTVILGMLDRELPHLELAKKEIISRGYEPVVRNFGGLAVVADEGILNFS
                     LVIPDVFERKLSISDGYLIMVDFIRSIFSDFYQPIEHFEVETSYCPGKFDLSINGKKF
                     AGLAQRRIKNGIAVSIYLSVCGDQKGRSRMISDFYKIGLGDTGSPIAYPNVDPEIMAN
                     LSDLLDCPMTVEDVIDRMLISLKQVGFNDRLLMIRPDLVAEFDRFQAKSMANKGTVSR
                     DE"
     gene            6106..6807
                     /gene="coaB"
                     /locus_tag="VBE22_000564"
     CDS             6106..6807
                     /gene="coaB"
                     /locus_tag="VBE22_000564"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEO7202038.1"
                     /translation="MYNMAMKILITSGGTTEKIDTVRSITNHATGALGKIIAEKYLRE
                     GHQVTLVTTKNAVKPESATNLSIFEIEDVDSLIKTLKPLVKEHDILIHSMAVSDYTPV
                     YMADFEKVKSSDDLDTFLRKDNHEGKISSESEYQVLFLKKTPKVISLVKKWNPQITLV
                     GFKLLVNVTKENLFKVARHSLIKNKATFILANDLIDITSKHHIAYLLDHDNVYKATTK
                     EDIAQLIYEKVKKYD"
     gene            6800..7342
                     /gene="coaC"
                     /locus_tag="VBE22_000565"
     CDS             6800..7342
                     /gene="coaC"
                     /locus_tag="VBE22_000565"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEO7202039.1"
                     /translation="MIKRITLAVTGSISAYKAADLTSQLTKIGYDVHIIMTQAATEFI
                     TPLTLQVLSKNPIHLDVMDEHNPKIINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALAMPDETPKLIAPAMNTKMYHNTITQRNINILKKIGYQEIEPRISLLACGDT
                     GQGALADISTILKCIQEVAN"
     gene            7388..7960
                     /locus_tag="VBE22_000566"
     CDS             7388..7960
                     /locus_tag="VBE22_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7202040.1"
                     /translation="MKKKNKSSNIAIIAIFFAIMLVIHFLSSFIFSFWLVPIKPTLMH
                     IPVIIASIAYGPRIGATLGALMGGISVANSSIVLLPTSYLFSPFVENGNFYSLIIALV
                     PRILIGIIPYFVYKLLHDRFGLAISGAIGSLTNTVFVLSGIFIFFSSTYNGNIKLMLA
                     GIISSNSLAEMVIAAIIVYLTVPRILNIKH"
     gene            8070..9788
                     /locus_tag="VBE22_000567"
     CDS             8070..9788
                     /locus_tag="VBE22_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO7202041.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSAGLIQVIGEDVDIEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATVGNDLADAETKIANIEKE
                     ITTFVN"
     gene            complement(9841..10038)
                     /locus_tag="VBE22_000568"
     CDS             complement(9841..10038)
                     /locus_tag="VBE22_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202042.1"
                     /translation="MKKRLQLLLLIFISAVSWFISNQLATKNTVLSVLIGLVAVIGIT
                     WLIVSLLYYLIVKLIAVIFHD"
     gene            complement(10052..11785)
                     /locus_tag="VBE22_000569"
     CDS             complement(10052..11785)
                     /locus_tag="VBE22_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202043.1"
                     /translation="MKASHPKKIQKKLVQDLLSKKSLVGMALLGTVVQVCLTVYLPVL
                     IGQAVDVVLSPHSMILLLPIMWKMVAVILANTIIQWINPLLYNRLIFHYVASLRKAVM
                     EKLNLLPIAYLDKRGIGDLISRVTTDTEQLSNGLLMVFNQFFVGLLTILVTIFSMAKI
                     DLLMLFLVLFLTPLSLFLARFIAKKSYHLYQNQTASRGRQTQFIEEMVSQESLIQAFS
                     AQEESSDHFRTINQEYANFSQSAIFYSSTVNPSTRFINSLIYGFLAGIGALRIMSGAF
                     SVGQLITFLNYVNQYTKPFNDISSVLSEMQSALACAERLYSILEESSPNITGTEKLDS
                     STVKGQIDFKNVIFGYNKSKPLLNGINLHIPAGAKVAIVGPTGAGKSTLINLLMRFYE
                     VDGGNILLDGKPITDYEPSQLRQEIGMVLQETWLKSATIHDNIAYANPKASRDEVIEA
                     AKAANADFFIKQLPNGYDTYLEDAGDSLSQGQCQLLTIARIFLKLPRILILDEATSSI
                     DTRTEVLVQEAFQMLMKGRTSFIIAHRLSTIQTADIILVMVSGEIVEVGNHSELMAQK
                     GIYYQMQNAQK"
     gene            complement(11786..13507)
                     /locus_tag="VBE22_000570"
     CDS             complement(11786..13507)
                     /locus_tag="VBE22_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202044.1"
                     /translation="MKRLTYYFKGYIKETIFGPLFKLLEASFELLVPIVIAKMIDETI
                     PRGDRSSLLLQIGLIFFLAAVGVVVAITAQYYSSKAAVGYTRQLTEDLYQKVMSLGKK
                     DRDELGTASLITRLTADTFQIQTGLNQFLRLFLRAPIIVFGAIIMAFSISPSLTIWFL
                     IMVVTLFIIVFVMSRLLNPIYLKIRTLTDYLVKLTRQQLQGVRVIRAFNQVDRESEAF
                     NDINYHYTNLQLKAGRLSSLVTPLTFLVVNIILVVIIWRGNLNIANHLLSQGMLVALI
                     NYLLQILVELLKMTMLVTSLNQSYISAKRIIAVFERPSEIIDDKLEPKYSNKALEVQE
                     MAFSYPNSSEKALSDITFSMNVGETLGIIGGTGSGKSTLVNLLLHIYKVQEGDIDIYH
                     QGKSPDTISNWRSLVRVVPQNAQLFKGTIRSNLSLGLGKVSEEKLWTALEIAQASDFV
                     KEKDGQLDAPVESFGRNFSGGQRQRLTIARALVQDKIPFLILDDATSALDYLTEARLF
                     KAIIKHFNQTNLIIVSQRINSIQNADRILLLDKGKQVGFDNHQSLLAHNKVYKSIYHS
                     QNFKEEE"
     gene            complement(13519..14121)
                     /locus_tag="VBE22_000571"
     CDS             complement(13519..14121)
                     /locus_tag="VBE22_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEO7202045.1"
                     /translation="MFKFLKRLIALIIIIFIGYRLVIIHENVKKVLQYHDLVQNTLAE
                     NGSEANVYLVLSMIYTETKGDAIDVMQSSESISGTTNSITDSHTSIKHGVTLLSQNIS
                     QAKKAKVDVWTAVQAYNFGSSYIDYVADHGGENSIELAKNYSKNVVAPSLGNYNGDTY
                     FYYHPLALISGGKLYKNGGNIYYSREVQFNLYLIKIMELF"
     gene            complement(14123..15100)
                     /locus_tag="VBE22_000572"
     CDS             complement(14123..15100)
                     /locus_tag="VBE22_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEO7202046.1"
                     /translation="MMDFYIKQIIIHQFSPNDTELVLSDTPLTLTPRIDDYFRKKLSK
                     VFSDEAKRGYFGEDNVFMSHLQDDLYVSSCQIAQLWKEEFVISEDQKTNDLVFIQFDK
                     DGMEHFAFLRISLKEQFAHVSENQEQPITITQNNLPSAAQTPDEALVVNKSSKQYYLI
                     EKRIKHNGSFANYFSENLLQVQPEQSVKKSIKMVEQTAQKIAENFNKDDFSFQSKMKS
                     AIYKNLEEEQELSPEKLADQLFDDNLTARLTFVDELKDAIPGSVQVSDIDHSRQIKKL
                     ENQKLSLSNGIELIVPNNVYQDAESVEFIQNPDGTYSILIKNIQDIQNK"
     gene            complement(15105..16361)
                     /locus_tag="VBE22_000573"
     CDS             complement(15105..16361)
                     /locus_tag="VBE22_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEO7202047.1"
                     /translation="MIFDKDNFKEFDQELWQAIHDEEIRQQNNIELIASENVVSKAVM
                     AAQGSVLTNKYAEGYPSHRYYGGTDCVDVVESLAIERAKTLFNAEFANVQPHSGSQAN
                     AAAYMALIEPGDTVLGMDLAAGGHLTHGASVSFSGKTYHFVSYSVDPKTEMLDYDNIL
                     KIAQETQPKLIVAGASAYSRIIDFEKFRQIADAVDAYLMVDMAHIAGLVASAHHPSPI
                     PYAHVTTTTTHKTLRGPRGGLILTNDEAIAKKINSAVFPGLQGGPLEHVIAAKAVALK
                     EALDPSFKIYGEDIIKNAQAMAKVFKEDDDFHLISDGTDNHLFLVDVTKVIENGKKAQ
                     NVLEEVNITLNKNSIPFERLSPFKTSGIRIGTPAITSRGMGVEESRRIAELMIKALKN
                     HENQDVLTEVRQEIKSLTDAFPLYEN"
     gene            complement(16453..17049)
                     /locus_tag="VBE22_000574"
     CDS             complement(16453..17049)
                     /locus_tag="VBE22_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEO7202048.1"
                     /translation="MNDLGQILEDHGAVIMPTETVYGIFAKALSEEAVNHVYELKKRP
                     RDKAMNLNICDFETILKYSKNQPTYLKQLYDAFLPGPLTIILEASQEVPHWINSGLLS
                     VGFRMPKHPVTLDMIANHGPLIGPSANISGCDSGRVFSEIQKQFNHQVLGIEDDKALT
                     GVDSTIIDLSGDRVKILRQGAITQEVLTATIPELIFEN"
     gene            complement(17042..17872)
                     /gene="prmC"
                     /locus_tag="VBE22_000575"
     CDS             complement(17042..17872)
                     /gene="prmC"
                     /locus_tag="VBE22_000575"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEO7202049.1"
                     /translation="MNYAQLIKHYGQLLEACGEEVENFIYVLKDLKQWSTTDYLLNQN
                     SSVSDTDQQLMENIFQQLKKHRSPQYITGKAYFRDLIFFVDERVLIPRPETEELVDLI
                     LSENKVEDCSVLDIGTGSGAIAISLKKERPSWDVLASDISVSALDLAKENANNCDAEV
                     TFIESDVFSNISGKFDIIVSNPPYISYNDKDEVGKNVLASEPHSALFADEEGLAIYRK
                     IIENSREYLQPRGKLYFEIGYKQGDDLRSLLKRYFPNNRCRVLKDIFGKDRMVVLDNE
                     "
     gene            complement(17872..18951)
                     /gene="prfA"
                     /locus_tag="VBE22_000576"
     CDS             complement(17872..18951)
                     /gene="prfA"
                     /locus_tag="VBE22_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEO7202050.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQNISDAEEMIKDASGDAELEEMAKEELKESKAAKEEYEERLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYEI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTQKLEALNK"
     gene            complement(18986..19555)
                     /locus_tag="VBE22_000577"
     CDS             complement(18986..19555)
                     /locus_tag="VBE22_000577"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEO7202051.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     EFGVVSSRIGMRREAVPISDDMDIFSYIQNLPQKPYCVLIDECQFLSKKNVYDLARVV
                     DDLDVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPDI"
     gene            19693..19875
                     /locus_tag="VBE22_000578"
     CDS             19693..19875
                     /locus_tag="VBE22_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEO7202052.1"
                     /translation="MPFVKIDLFEGRSQEQKNELAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGELKKK"
     gene            20024..20962
                     /locus_tag="VBE22_000579"
     CDS             20024..20962
                     /locus_tag="VBE22_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEO7202053.1"
                     /translation="MQLSHSLRLMGTTIDIQINSKNAQKQIQEVIELLELYKNRFSAN
                     DFNSELMAINNNAGIKPIQVHPDLFELITIGKEHSLARPSNLNIAIGPLVQTWRIGFS
                     DAKLPSPSEISEAMILSDPTHILLDSKNQSVFLNQIGMKIDLGALAKGYIADKIMTYL
                     KNEMIDSAIINLGGNVLVHGDNPNRSEGYWVIGIQHPKKKRGQNIGAVKIKNQSVVTS
                     GTYERRLIIDDKEYHHIFDRQTGYPIQTEMASISIVSKQSVDCEIWTTRLFGLSIKEA
                     LDILNAVSYIEGIIITKDDRIYLSDGLKHHFQLFYH"
     gene            20980..21582
                     /locus_tag="VBE22_000580"
     CDS             20980..21582
                     /locus_tag="VBE22_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7202054.1"
                     /translation="MKLIGIVGTNSNKSTNRQLLQYMQQHFADKAEIELIEVKDLPLF
                     NKPADKNVPQVILDIAAKIEEADGVIIGTPEYDHSIPSALMSVLAWLSYGIYPLLNKP
                     VMITGASYGTLGSSRAQLQLRQILNAPELKASVLPDEFLLSHSLQAFDKDGNLHDIET
                     SQKLDAIFDDFRLFVKITGKLSNARDLLQKEAENFDWESL"
     gene            21606..22841
                     /locus_tag="VBE22_000581"
     CDS             21606..22841
                     /locus_tag="VBE22_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7202055.1"
                     /translation="MKFVGIVGSNAEQSYNRMLLEFIRKNFKTKFELEVLEIDDIPMF
                     NQDQNWEESFQLRLLNNKITRADGVIIATPEHNHTITAALKSVLEWLSFAVHPLENKP
                     VMIVGASYYDQGTSRAQIHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDDNGNIINPG
                     TVQFLETCLDNFIKYVGVVSSLRKPKAIESEDLYCQGQIDTTITGIDPDDPDWLDKAA
                     EKVNAVEGDAYVKLDNGLLTVNQLNMFLNAMPFELTYADDNNQFLYYNNNHPDPDTML
                     GKRVREQVGNRMATVHGSLPPARMKNVEWVIGTLRNGNQEYVRTVVPGTPPEVINTHN
                     YQAMYYPDGSYAGINEIIFNFKPWLDWYLQTTGQRLVGGTTAAPGASAVDATSSASTS
                     TGDAPVDQATDATSGASEH"
     gene            22940..23728
                     /locus_tag="VBE22_000582"
     CDS             22940..23728
                     /locus_tag="VBE22_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEO7202056.1"
                     /translation="MSLFQEKIAYNCAKKEALYKESLGRYALRSMLAGAYLTMSTAAG
                     IVAADTIGKISPALSGFVFAFIFSFGLIYVLIFNGELATSNMLYLTAGAYNKNISWKK
                     AMTILIYCTFFNLVGACILAWLFNQSYSFQHLTNDSFLGHVVAKKLRKPSSGAFLEGI
                     IANMFVNLAILAYMLLKEESAKMTVILSAIFMFVFLSNEHLIANFASFMLAAFSHIEH
                     IKGFTLLNVIRQWTLVFFGNWIGGGVFIGLAYAWLNKTKTSHHE"
     gene            complement(23827..25101)
                     /locus_tag="VBE22_000583"
     CDS             complement(23827..25101)
                     /locus_tag="VBE22_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEO7202057.1"
                     /translation="MKFNEQSNSQAALLGLQHLLAMYAGSILVPIMIASALGYNAKQL
                     TYLIATDIFMCGIATLLQLRLSKHFGVGLPVVLGCAFQSVAPLSIIGAQQGSGYMFGA
                     LIASGIYVVLVAGIFSKVANFFPPIVTGSVITTIGLTLIPVAMGNMGDNAKEPSLQSL
                     TLSLVTIGVVLLINIFAKGFLKSISILIGLISGTILAAFMGLVDASVVAEAPLVHIPK
                     PFYFGAPRFEFTSILMMCIIATVSMVESTGVYLALSDITNDKLDSKRLRNGYRSEGLA
                     VLLGGLFNTFPYTGFSQNVGLVQISGIRTRKPIYFTALFLVILGLLPKFGAMAQMIPS
                     PVLGGAMLVLFGMVALQGMKMLNQVDFEHNEHNFIIAAVSIAAGVGFNGTNLFISLPN
                     TLQMFLTNGIVISTLTAVVLNIILNGLPKKLI"
     gene            complement(25101..25682)
                     /locus_tag="VBE22_000584"
     CDS             complement(25101..25682)
                     /locus_tag="VBE22_000584"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEO7202058.1"
                     /translation="MKLLEERILKDGDVLGENILKVDSFLTHQVDFELMQEIGKVFAD
                     KYKEAGITKVVTIEASGIAPAVYAAQALGVPMIFAKKAKNITMTEGILTAEVYSFTKQ
                     VTSQVSIVSRFLSNDDTVLIIDDFLANGQAAKGLLEIIGQAGAKVAGIGIVIEKSFQD
                     GRDLLEKTGVPVTSLARIKAFENGRVVFAEADA"
     regulatory      complement(25786..25881)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(25937..26920)
                     /gene="guaC"
                     /locus_tag="VBE22_000585"
     CDS             complement(25937..26920)
                     /gene="guaC"
                     /locus_tag="VBE22_000585"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEO7202059.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIISSRSQADTSVKLGNYTFKLPVI
                     PANMQTIIDEEVAETLACEGYFYIMHRFNEEERKPFIKRMHDKGLIASISVGVKDYEY
                     DFVTSLKEDAPEFITIDIAHGHSNSVIEMIQHIKQELPGTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCSKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHLESPGKLVEVEGQQFKEYYGSASEYQKGEHKNVEGKKIL
                     LPVKGRLEDTLTEMQQDLQSSISYAGGKELDSLRHVDYVIVKNSIWNGDSI"
     gene            complement(27122..28462)
                     /locus_tag="VBE22_000586"
     CDS             complement(27122..28462)
                     /locus_tag="VBE22_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7202060.1"
                     /translation="MTKKGWRIMPAIITTAILGFSGILIETSMNVTFPLLMKEFGVNP
                     AVIQWVTTGNLLAVAVTVPLSAFMIKNLSERQIFTLANVLFLSGVLIDSFAPNLAILL
                     VGRVLQGVGTGLALPLLFHIILTQIPMERRGLMMGVAAMVTLLAPAVGPTYGGVISGM
                     LGWKMIFMLLAPILIISTFIGLASIPKRQVRINDKLNFPAFVSLGIGLATLLLAIEKV
                     SIFYLLVAIVSFVIFYYLNKQLEFLNLNVFKDKDFSILLYGVLAFQMIPLALSFLLPN
                     LLQLVLHQTSTKAGLFMFPGAIAVVFLSPFAGYLLDKIGAFKPIMIGISLSLIGLIGT
                     AIFIPAKSVVVLLAFDILTKIGMGIGASNMVTTALTKLKPTQSADGNSILNTLQQFAG
                     AFATAVASQIFTIGQVAIPKNGAIIGSQFAVLFVIVVVILAIVGLTYLRKRKAI"
     gene            28754..29425
                     /locus_tag="VBE22_000587"
     CDS             28754..29425
                     /locus_tag="VBE22_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202061.1"
                     /translation="MAFASNESENNFFITLKTYFNYLFSIQIITDISTLNHADFDGSF
                     AFHDIETSIPHLVIDSNYLAISQTNSKTEANDIKTFSELSKTMTEFHYMLNFDLFNHL
                     PYRFRLHNKDGQTIYSNHKPEDPFDIYPEEEYPIDKWVQNSLIEKKAKELHLLLPSAS
                     QDYILVQSYKRLENDSGQLVGYIEHVHNIKPLLEGYLKESGQAIVGWSDVTSGASISN
                     DDFEL"
     gene            complement(29497..31497)
                     /locus_tag="VBE22_000588"
     CDS             complement(29497..31497)
                     /locus_tag="VBE22_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO7202062.1"
                     /translation="MQHVNHSSFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GISSVTESFILGSISLIIWTLTLITTIKYVLVALKADNHHEGGIFSLYTLVRKMTPWL
                     IVPAVIGGATLLSDGALTPAVTVTSAVEGLKVVPSLQHIFQNQSNVIFATLFILLLLF
                     AIQRFGTGVIGKLFGPIMFIWFAFLGISGLLNSFAHPEVFKAINPYYGLKLLFSPENH
                     KGIFILGSIFLATTGAEALYSDLGHVGRGNIHVSWPFVKVAIILSYCGQGAWILANKN
                     AGNELNPFFASIPSQFTMHVVILATLAAIIASQALISGSFTLVSEAMRLKIFPQFRST
                     YPGDNIGQTYIPVINWFLFAITTSIVLLFKTSAHMEAAYGLAITITMLMTTILLSFFL
                     IQKGVKRGLVLLMMIFFGILEGIFFLASAVKFMHGGYVVVIIAVAIIFIMTIWYKGSK
                     IVSRYVKLLDLKDYIGQLDKLRHDHRYPIYHTNVVYLTNRMEGDMIDKSIMYSILDKR
                     PKKAQVYWFVNIKVTDEPYTAEYKVDMMGTDFIVKVELYLGFKMRQTVSRYLRTIVEE
                     LLESGRLPKQGKTYSVRPDSKVGDFRFIVLDERFSSSQNLKPGERFVMLMKSSIKHWT
                     ATPIRWFGLQFSEVTTEVVPLIFTANRGLPIKEKIELTTTGD"
     gene            complement(31632..32393)
                     /locus_tag="VBE22_000589"
     CDS             complement(31632..32393)
                     /locus_tag="VBE22_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEO7202063.1"
                     /translation="MISRKVALVTGASAGFGAAIVTKLVSDGYSVIGCARRMDKLKCL
                     GEKFSEGYFYPLQMDITSRESVDKALESLPKNLQSIDLLVNNAGLALGLDKSYEADFG
                     DWMTMINTNVVGLIYLTRCILPKMVEVNRGLIINLGSTAGTIPYPGANVYGASKAFVK
                     QFSLNLRADLAGTKIRVTNLEPGLCEGTEFSTVRFKGDHKRVEKLYEGAHAIQAEDIA
                     NTVSWVASQPEHININRIEIMPVSQTYGPQPVYRD"
     gene            complement(32455..33447)
                     /gene="pta"
                     /locus_tag="VBE22_000590"
     CDS             complement(32455..33447)
                     /gene="pta"
                     /locus_tag="VBE22_000590"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEO7202064.1"
                     /translation="MRSLFGGLREKVASDYVKIVFPEGSDERVLRAAARLKFEGLVDP
                     VILGKADEVHDSLARLGFVDQDYSVIDPEQYEKFEEMKEAFVEIRKGKATMEDADRLL
                     KDVNYFGVMLVKLGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRENT
                     QERYIFADCAINIDPNAQELAEIAVNTADTAKIFDIDPKIAMLSFSTKGSAKAPQAEK
                     VQEAAKIAKDLSPELAVDGELQFDAAFVPETAEIKAPNSDVAGKANVFIFPDLQSGNI
                     GYKIAQRLGMFEAIGPILQGLNAPVNDLSRGSSAEDIYKLAIITAAQAIDEKSK"
     gene            complement(33473..34357)
                     /locus_tag="VBE22_000591"
     CDS             complement(33473..34357)
                     /locus_tag="VBE22_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO7202065.1"
                     /translation="MKFEYIAEERCKVKTLLKSHDVSRGLLAKIKYRGGKIFVNGEEQ
                     NAIFLLEIGDVVTIDIPDEPSHETLEPVPHDLDIVYEDDHFLILNKPFGFASIPSSIH
                     SNTIANFIKHYYVSNNYANQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQAKAIE
                     KRYYALVSGSGDLADSGDIIAPIARDVDSIITRRVHESGKYAHTSYQVVARYGDVRLV
                     DIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRMDLGINRQALHCHSLSFYDPFMGKIN
                     KQTLDLTDDFDSVIMELH"
     gene            complement(34354..35190)
                     /locus_tag="VBE22_000592"
     CDS             complement(34354..35190)
                     /locus_tag="VBE22_000592"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEO7202066.1"
                     /translation="MTQMNFTDRATRVAIIANGKYQSKRVASKLFAAFKHDPDFYLSK
                     KDPDIVISIGGDGMLLSAFHMYEKQLDKVRFVGVHTGHLGFYTDYRDFEVDTLINNLK
                     NDKGEQISYPILKVTITLEDGRIIRARALNESTIKRIEKTMVADVVINQVVFERFRGD
                     GILVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVYRTLGSSVIIPKKDAIE
                     IVPKRVGVYTISIDNKTVHYKNVTKIEYSIDEKSINFVSTPSHTSFWERVNDAFIGEP
                     EH"
     gene            complement(35165..35836)
                     /locus_tag="VBE22_000593"
     CDS             complement(35165..35836)
                     /locus_tag="VBE22_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO7202067.1"
                     /translation="MSMDWETFLDPYIQTVGELKIKLRGIRKQFRKQNRHSPIEFVTG
                     RVKSVESIQEKMVLRGISEENLAQDLQDIAGLRIMVQFVDDVDEVLALLRKRHDMTVV
                     QERDYITHMKSSGYRSYHVVVEYPVDTIDGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPEEIKQRLEKTAKIALELDEEMRKIREDIREAQLLFDPLNRKLSDGVGNSDDT
                     DEFYR"
     gene            35946..36518
                     /locus_tag="VBE22_000594"
     CDS             35946..36518
                     /locus_tag="VBE22_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEO7202068.1"
                     /translation="MTHLEIEYKTLLNKDEFNRLTSLFSHVQPITQTNYYFDTETFEM
                     KAHRMSLRIRTLPNRAELTLKIPREVGNLEHNHDLTLEEAKYIVKNGQFPEDTEIASL
                     ILEKGVDPTKLAVFGQLTTTRREMETSIGLMALDSNIYADIKDYELELEVKQPKQGKR
                     DFDQFLKENNINFKYAKSKVARFSATLKNN"
     gene            36695..37669
                     /locus_tag="VBE22_000595"
     CDS             36695..37669
                     /locus_tag="VBE22_000595"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO7202069.1"
                     /translation="MAEQYADKQIKLFSLTANREIAEKISQASGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVVPYFGYSR
                     QDRIAASREPITAKLVANMLVKAGVDRVLTLDLHAVQVQGFFDIPVDNLFTVPLFAEH
                     YNQLGLSGEDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREEGYIIGEVEG
                     KKAIIIDDILNTGKTFAEAAKILERGGATEIYAVASHGLFAGGAADILESAPIREIIV
                     TDSVLSKERIPSNIKYLTASHLIADAIIRIHERKPLSPLFSYRSDKKD"
     gene            37673..38788
                     /locus_tag="VBE22_000596"
     CDS             37673..38788
                     /locus_tag="VBE22_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7202070.1"
                     /translation="MIYLDNAATTALTPSVIEKMTNVMTSNYGNPSSIHTFGRQANQL
                     LRECRQIIAEYLNVNSREIIFTSGGTESNNTAIKGYALANQLKGKHIITSEIEHHSVL
                     HTMTYLSERFGFDITYLKPNHGQITAKDVQEALRDDTIMVSLMFANNETGDFLPIQEI
                     GQLLRNHQAVFHVDAVQVFSKMELDPHSLGIDFLAASAHKFHGPKGVGILYCAPHHFD
                     SLLHGGDQEEKRRASTENIIGIAGMSQALTDATTNTLKNWTHISQLRTTFLDAISDLD
                     FYLNNGQDCLPHVLNIGFPGQNNGLLLTQLDLAGFAVSTGSACTAGTVEPSHVLTSLY
                     GANSPRLNESIRISFSELNTQEEILKLAKTLRKIIGD"
     gene            38790..>38899
                     /locus_tag="VBE22_000597"
     CDS             38790..>38899
                     /locus_tag="VBE22_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7202071.1"
                     /translation="MAFEKEINLPDCNYIYTISPNIKKYTLRDTTFTQNR"
BASE COUNT        12778 a         7522 c         6174 g        12425 t
ORIGIN      
        1 caatgagaag gttttcaata tctgaaatat ctttagaact gaaaaagtca ccataaatac
       61 ggcaattcgt aataatacct ttctcaattt ctaagtgaaa atcgtaagtt cctgacggaa
      121 aacgagcatc tctgtgatac gaatactgtg gtgagttacc ataattccaa tcccaattct
      181 tatatttttc atcagctaga gcgtcaatcg cagcccaatc tttttcggtt agatgatagc
      241 gttttgcctg atcaatatga tcaatatgta ataattgaca aaccattaaa tctttaaatt
      301 gatcggttgt gatatgacga taatcttctg acaagtggct acgaatatct cctactcgac
      361 ttcggacaga ttttatccct ttagattcaa tttttttccg attaggattt aacacttttt
      421 ccatagcatc aaaatcaaca tctaataaaa gagagtaacc accgtaaata cgaccgttta
      481 ctatcgtcat tgctgcacct gatacttttt taccatcaat aactaagtca tttcgtcccc
      541 tcatttcaac actagaagca cctaaagttt ttagggcctt aatagctggc tcataagctc
      601 gtttgaaatc accaaattgt ccatgatctt tcatgagata gcaaatattt acagcacctg
      661 agtcaacgta aacagcccct ccaccagtat ctcgacgaac aacaggaata tcatgttctt
      721 tcaaatattc ttgatttgtc tctataactg cattctgaaa ttttccgatt tcaactttgg
      781 gatcacaata gtatggaaat aaaatatcat catctaaaaa aacattttgt aaaatataaa
      841 cttgcatggc aagtgcaatc gcaccgtctg tgatacgctt accatttcta attggttcaa
      901 taagatacat ttaggtctcc ttttatagca atttagttat ccaataattt cttttttcgc
      961 ttcagcaatt aattcagcga tatgctgtgt cagttgcaca ctccttgagc gaatcttttg
     1021 aggaatgcga taatgcttgt gattaagagt aacaaataaa gagttttcat ttaaagagac
     1081 gaatcgccaa aacggatgct taataaattg tggcgtggta tgtccaactc caatctctag
     1141 ataaagaact ttgtcatttt gatgttgtga taagaaattt tcataacgtt gtcgttgagc
     1201 atgaaagtca gcatcttcca ccataccttt ttcttcgtta cgtttattaa tttcaaacgg
     1261 ttgatcacac tttgggcatt ttgggattaa gttactagga atcttcatat ccttttgttg
     1321 tgcaatcatt tgacgaattg cctgatcatt tcgataagtt tgttgatgac aatgttgact
     1381 acactgccat aagccatatt caccttgaat atgaaaaact ttttctaaat tataatcagt
     1441 cacagcgaag gcattatcag cattagttgt aataatatga tattcttttt tcgctaaaat
     1501 atcctttaaa tcaagataag cttgtcctac cggttgatct aaataattaa gtgccacaaa
     1561 acgactttgg aaggcccaat attcttccca atcttcaaag tcataaagac ttgcttgcaa
     1621 catatctaat agttgatatt tagcaataaa atccggaaaa gcttcttcaa atctggggcc
     1681 aatataggta aatccatccg ctgcagacat gcctgcacca ataccaacta caatagcatc
     1741 tgattcttct attaactgac ttagtatttc tgattgagaa tgattagttt tttctagagt
     1801 cttccacact gtcatctatt tcctctttta ataataattt ttgataaatc tcttggtctt
     1861 taggtgtgaa tgtattgaaa ataatataga gtttgtgctg attttcgcgt tgccactcta
     1921 atatcgcttg aacagccaat tcagcagctc tttgattagg aaaaccaaat tctcccgttg
     1981 atatacaagg gaagacaatt gttttaagat tagctcgaac tgccaactgt aagcaggatt
     2041 tataagaaga ctttaataaa tcctctctta tacgagatgg cttttgatca acttttacgt
     2101 aaggtccgac tgtatgaata acatacttac aaggaagatg ataggattcg gtaagtttag
     2161 cttgtcctac ggcttccatt cgtccttgtt gtgtcatcaa ttgatgacat gctaaacgta
     2221 atcgtgaacc tgcaaacgta tgaatatgat tgtcaataca atggtgatta ggaataaaac
     2281 aacctaacaa cttactattt gctgcattaa caatagcatc cacttgtaat aagcaaatat
     2341 ccccatggta ggtaaataca tttgaatata ccgtttgatg acagacgtct atcgttttta
     2401 ctttttgagt attccaccaa tgtgacaaat acctatcttc taaagacagg tactcttgac
     2461 taatgttttg aggatcttgt tgattaacta gaccacgcca aaccgtttgc aaatcggtgg
     2521 tcctcaagtc atcaacgtct gttaaatttt ctgactgaag atactcaatc atggctagca
     2581 atagttgttt ttgattaggc attttctaaa gcttcaaaag cagcataatc aacgtctgtt
     2641 aacacaacca accaattttc gtcagctttt tcagaattta acaaagttgg ttcttgactt
     2701 gcagctgtat taactttaac aactttacct gtaagaggag ataaaattgc cataaccgtc
     2761 tttgaagctt caatatttaa aataacatca tcaacttcta aatttgtatc atctgtgaat
     2821 tccacatatc cgacagttcc caagtcatct tgaagttctg gagttaaact gatagtataa
     2881 agttcttcat ttttttcaat taataagtag ttcgcaattt ttttcatgag taatttcctt
     2941 tctgagaaaa cgtgattaat ataactgtga taataatttt aaggatttga ttcgttgatc
     3001 aagtcctgga acaagaggaa tcaaaagtag ttcttctgct tgactagcat taactaaagc
     3061 gtcaaggctt tctttaactt gttttggatc acctacaatc atacggtggc gatttgattt
     3121 aatttttgct ttttgttcag gagtcaattg gtaatgatta gcttcttcaa tagtaggaaa
     3181 tgttgcaaat tcattgaagt ctttatttcc tagcatccag atatctaaag tctttgctat
     3241 gttttcagct tcttctgaag tatcagctat aactacaaat gcagctacca tcaaattagg
     3301 agacttatta ggataatatt cttcaaactg atgataataa agagatgaaa gctttttagc
     3361 ttctgtcaat gggtctttgt ccataaaagg aaaaacaccg aaggtaaagc ctaagccaag
     3421 tttagccgct aaataagctg atttttgacc actccccaac acatataagt ctgggaagct
     3481 agaaagagtc ggttgaacta atggtttctt gctggatgat tcatcaataa gccatgactt
     3541 caattcctcc agtacctctt cgtaatcatg tgctttatgt aagctacgaa gtgcatttga
     3601 aactttaact gtccctagtg aatttcctaa accaatactt actcgattcg gatgacatgt
     3661 ctctaatgtt ttgagagtct ccgcgagttt aaaactacta tagtgcagag gcattatacc
     3721 tccagagcca attttgatag atttagtctg gttagccaaa tgcattatca ttaattctgg
     3781 attgctaata ctgaatgccc taacaccgtg atgttcagcc acccaaaatt gatgatagcc
     3841 tagtcgctcc gcctcttgtg ataaaagaat cgtttcttgt attgcatcac ttgcagtttt
     3901 tccataatca ataagcccat aatcaaggac acttaatttc atctttcctc cctataattg
     3961 cttcgaagtt ctctaaaaac ttctcgagct tcctttggca aagattctcc ataatccatc
     4021 aaaggaacaa tatctttaaa ggaggctttt ggaatcgcca aaccttctaa atcactcact
     4081 ttaatcctta aattaatctg atcagaacgc ttagctttta ttttttgtaa aaattccgga
     4141 tcaactacta agggcgtgga agctcctacc atatgagcat gttgtaaggc ttcaaacacc
     4201 tttgaagcac tattaatacc tccagttgcc ataattggaa ctctatcacc aaaatgttca
     4261 aatataactt gattaaccag ctgccctttg taaactccct cagaacgtat agtatttcta
     4321 aagacatcat gcccccagct tgcaacggca agataatcta cctgagcaat cgctaaaatt
     4381 ttttcaagaa attccataaa ctcttcaatg ctatatccaa tttgtgaacc gcgagtttct
     4441 tcaggcgtcg ctctaaaacc aaggataaaa tctgactctg cttcttctct gataactttt
     4501 tgtaccgctt taaaaacttc taagcctaaa cgacagcgat tagtcaaagt ttgaggacca
     4561 tactcatctt ttctttgatt tgagaaggtt gagaaaaagg tttgaattag taatcgttgt
     4621 gcagaagata tttcaacacc atcaaatcca gcttgtatag ctcgtcttgt agcctggaca
     4681 tattcatcga taatccttag tatatctttg tgtgttagtt ctttaacctg atgtgggtat
     4741 ggcgattgca attgcatagg gctgggacca tacacatatc cgtgtcttgc caaagtgtga
     4801 cttgagaaac gtccagcatg cgtcagctgc aaaacagctt tcgctccctt agacttcatt
     4861 gctttagcca atttagtcaa accaggaatg tcctcatcct ttgaaacgct aaaaccatat
     4921 tcaaataact gaccatattc tgtgatatat gctgctccag taatttgtaa tggagcagat
     4981 ttagcacgac gaacagcata tgctatgtcg tcatcagtta caaaaccttc tgatgtagaa
     5041 gaattagtta ccataggtga tagaacaaaa cgattttcta agctcaatcc attgggaagc
     5101 gttaatggac gaaataaatt accttggaca ttattcatct ctgctcaccg tccccttatt
     5161 agccatagac ttagcctgaa atctatcaaa ctctgcaact aaatcgggcc taatcatcag
     5221 taaacgatca ttaaaaccta cttgtttaag gctaatcaac atacgatcaa taacatcttc
     5281 tactgtcata ggacaatcta atagatcaga tagattagcc ataatttcag gatctacatt
     5341 tggataagca attggactac ccgtatcacc tagaccaatc ttataaaaat ctgaaatcat
     5401 tcgactccgc cctttttgat cgccacaaac gctaaggtaa atcgataccg caataccatt
     5461 ctttatacgg cgctgagcca agccagcaaa ttttttgcca tttatactaa gatcaaactt
     5521 accaggacaa taggaggtct ctacttcaaa gtgctcaata ggttgataaa aatccgaaaa
     5581 tatacttcta ataaaatcga ccattataag atacccatct gagatagaca attttctctc
     5641 aaaaacatct ggaataacca atgaaaaatt taaaattcct tcatcagcta caactgcgag
     5701 accaccaaaa ttccgaacaa ctggttcata accacgactg atgatttctt tttttgctaa
     5761 ttctaaatgt ggtaattctc gatctaacat ccccagaatg actgtcttag tcatcggcca
     5821 aatatgcaaa ataatttctt gatttgagcg attaatctcc cttaaaaata cttctgtcca
     5881 tataaaaggt ttttccgcgt cttgagcatc tgtaacatag tctttaaaaa tagatacagg
     5941 taattgcgct agatcttgcc attccaataa atctgacctc ctttctctag aaaactctgc
     6001 aagatttatt ttatcgcaat cgcttgaaat tttccagtaa gtttccctca aattcaatta
     6061 ctatcaagaa gaaatatatc ttttatttac gaaaagcatt tttttatgta caatatggct
     6121 atgaaaattt tgattacatc aggtggaact accgagaaaa ttgataccgt taggagtatc
     6181 acgaatcacg caacaggagc tttaggaaaa ataattgcag aaaaatattt acgagaagga
     6241 catcaggtga cactcgtgac tactaaaaat gcagtcaaac cagaatctgc taccaactta
     6301 tccatatttg agattgagga cgttgactca ttaataaaaa ccttgaagcc attagtgaaa
     6361 gagcatgata ttttaattca tagcatggca gtgtctgact atacccccgt ctacatggct
     6421 gatttcgaaa aggttaaatc aagcgatgat ttagatactt ttttgcgcaa agataaccac
     6481 gaagggaaaa tatcatctga atcagaatat caagtccttt ttctcaaaaa aacaccaaaa
     6541 gttatcagcc ttgtgaaaaa atggaatcct caaattactc tagtaggttt taaactactt
     6601 gtgaacgtta ctaaagaaaa tctttttaaa gttgctcgtc atagcttaat caaaaataaa
     6661 gctacgttta ttttagctaa tgacctcatt gatattacct caaaacatca catagcctac
     6721 ctacttgatc atgataatgt gtataaagct accaccaaag aagatattgc tcaattaatt
     6781 tacgaaaagg taaaaaaata tgattaaacg tattacacta gccgtcaccg gttcaatttc
     6841 ggcctataaa gcagcagacc ttacaagtca attaactaaa attggctatg atgttcacat
     6901 cataatgaca caagctgcca cagaatttat tacaccgtta actctgcaag tattatcaaa
     6961 aaatcctatt catttagatg ttatggatga gcataaccca aaaatcatca accatatcga
     7021 attagcaaaa cgcacagatt tatttatcgt tgcacctgcc tctgctaata ctattgctca
     7081 tctagcatat ggcttcgctg ataacatcgt aacaagcgtt gctcttgcta tgcccgatga
     7141 aactccaaaa cttatcgcac cagcaatgaa tacaaagatg tatcataata caatcaccca
     7201 acgtaacatt aatattttga aaaaaatagg ttatcaggaa attgaacctc gtattagttt
     7261 gcttgcctgt ggtgataccg gtcaaggagc cctggctgat atctccacta tacttaaatg
     7321 tatacaagaa gttgcaaatt aggctgtaaa tttgtatact atttctagtt taatttaagg
     7381 agttataatg aaaaagaaaa acaaatcctc taacattgct ataattgcaa tcttttttgc
     7441 tattatgctt gtcattcatt ttttgtcatc atttattttt agtttttggt tagtccctat
     7501 taaacctact ttgatgcata tcccagttat tattgcatct atagcctatg gacctcgtat
     7561 tggtgcaact ctaggcgcct taatgggggg gatcagcgta gctaacagca gcattgttct
     7621 attaccaacg agttacctct tctcaccttt tgttgaaaat ggtaattttt attcgctaat
     7681 tattgcactt gtaccacgta ttctaatcgg gattattcct tatttcgttt acaaattact
     7741 acacgaccgc tttggtttgg ctatctcagg tgctataggc tctctaacaa acacagtatt
     7801 tgttttatct ggaattttta tctttttttc aagtacttat aatgggaata tcaagctaat
     7861 gctcgctggg attatttcat ctaattcatt agctgagatg gtcattgcag ctatcattgt
     7921 atatctaact gttcctcgta ttctcaatat taaacattaa aaactggcca ggaagaaatc
     7981 ttcctggctt ttcttactta tattttatag tcatattgaa aaaaacatgt tataatgaaa
     8041 gcgtttataa atattgaagg agtatttcaa tgacttacac agaaaatttt caaaaatggt
     8101 tagattttga gcagcttcct gactatcttc gtcaagaatt actatcaatg gatgaaaaaa
     8161 caaaagaaga tgctttttac acaaacttag aatttggtac tgctggtatg cgtggttata
     8221 ttggtgctgg tacaaatcgt attaatattt atgttgtccg tcaagcaacc gaaggattgg
     8281 caaaattaat cgaaactaag ggagaggaag ctaaaaaacg tggtgtagcc attgcttatg
     8341 attcacgcca cttctctcca gaatttgctt ttgagtctgc acaggtcttg gcacagcatg
     8401 gaattaaatc ttacgtattt gaagctcttc gtccaacacc agaactctct tttgctgttc
     8461 gccatttaaa tgcctatgct ggtattatgg ttactgcaag ccacaatcca gcacctttca
     8521 atggttataa agtctacgga caagacggag gtcaactacc accagctgat gctgatgcac
     8581 taacagactt tatccgtgct attgaaaatc catttgcagt tgaacttgct gatttagatg
     8641 aaagcaaatc agcgggtctt attcaagtta tcggtgaaga tgttgatatt gaatatctta
     8701 gagaggttaa agatgttaat atcaatcaag atttaatcaa taactttgga aaagacatga
     8761 aaattgtcta tactcctctc cacggaactg gtgagatgtt gacacgccgt gcccttgctc
     8821 aagccggttt tgaatctgtt gttgtagtag aatcacaagc taaggcagat ccagactttt
     8881 ctactgtcaa atctcctaac cctgaaagcc aagctgcctt tgctcttgct gaagaactcg
     8941 gacgagaagt tgatgcagat gtcttagtag ctactgatcc tgatgcagac cgcctaggcg
     9001 ttgaaattcg ccaaccagat ggttcttata aaaacctttc tggaaatcaa attggagcga
     9061 ttattgcgaa atacatcctg gaagctcata aaacagcagg aacacttcca gaaaatgctg
     9121 ctctcgctaa atcaatcgta tcaacagaat tagtaacaaa aattgccgaa agttatggcg
     9181 ctacaatgtt taacgtttta actggattta aattcatcgc tgagaaaatt caagagtttg
     9241 aagaaaaaca taaccacacc tatatgtttg gttttgaaga aagcttcgga tatcttatta
     9301 aaccatttgt ccgtgataaa gacgctattc aagcagttct tttggttgct gagattgcag
     9361 cttattaccg ttcgcgtggt ttaacacttg cagatggtat tgatgaaatt tacaaagaat
     9421 atggttattt tgctgaaaaa acgatttcag taacactttc aggagtggat ggagctgctg
     9481 aaattaagaa gattatggac aaattccgtg aaaatggtcc aaaacaattt aacaatactg
     9541 atattgtatt attagaagat ttccaaaaac aaaccgctac taaaaatgat ggaacaatat
     9601 ctaacttaac aactcctcca agtaatgttt tgaagtacac gttagctgat gattcatgga
     9661 ttgcagttcg cccttcagga actgaaccaa aaatcaagtt ctatattgcc acggtcggta
     9721 atgatttagc agatgcagaa acaaaaatcg ccaatattga aaaagagatt acaacatttg
     9781 taaattaata aaaaaagcta ggaacttact cctagctttt tttatatcta tctaacgatt
     9841 tcaatcatga aatataactg caattaactt cacgatcaaa taatagagca gacttacaat
     9901 taaccacgtt atcccaataa ctgcaactaa acctattaaa acagataata ccgtattctt
     9961 agttgctagt tggttactga taaaccatga aactgcagaa ataaatatca gtaataatag
    10021 ttgaagcctt tttttcatat aactcctctt actacttttg tgcattttgc atttgataat
    10081 aaataccttt ttgagccatt aattcagagt ggttacccac ttctacaatt tcaccagata
    10141 ccataactaa aataatatct gctgtttgaa tggtagataa gcgatgtgca ataataaagc
    10201 ttgttctccc cttcatcaac atttggaatg cttcttgaac aagaacttca gtacgagtat
    10261 ctatagacga tgttgcctca tctaatatca atattctagg taatttaaga aaaatacgag
    10321 caattgtcaa tagctgacac tgcccctgtg ataacgagtc tccagcatct tctaaatacg
    10381 tatcatatcc attcggtaat tgtttgataa agaaatctgc attagctgct tttgcagcct
    10441 ctatcacttc gtcacggcta gctttaggat tggcatatgc aatgttatcg tgaatcgtag
    10501 cactttttaa ccacgtttct tgtaatacca ttccaatttc ttgccttaat tgtgatggct
    10561 cataatcagt aataggctta ccatctaaaa ggatatttcc cccgtcaact tcatagaaac
    10621 gcattaataa attgattaaa gtagacttcc cagcacctgt tggtcctaca atagcgacct
    10681 tagctcctgc tggaatgtgt aaattaatac cattcaatag tggttttgat ttattataac
    10741 caaaaataac gtttttaaaa tcgatttgac ctttgaccgt tgatgaatca agtttttctg
    10801 tacctgttat atttggtgac gattcttcta aaatagagta aaggcgttct gcacaagcca
    10861 gagcactttg catctcagac aggacggaag agatgtcatt aaatggctta gtatattgat
    10921 tgacataatt taagaaagtg attaattgac caacactaaa agcacctgac ataatacgaa
    10981 gggcaccaat ccctgctaag aagccataaa tcaaactatt gataaatcgg gttgatggat
    11041 tgacagttga agaataaaaa attgctgatt gagaaaagtt agcatactct tgattaatag
    11101 tcctaaaatg atcactactt tcttcttgcg cactgaatgc ttgaataagg ctttcttgac
    11161 tcaccatctc ttcaatgaat tgagtttgtc gtcctcgtga tgccgtttga ttttgatata
    11221 agtgataact ctttttagca ataaagcggg ctagaaagag tgataaaggc gtcagaaata
    11281 ataccaaaaa aagcattaat aaatcaatct ttgccatact aaaaatagta actagaatag
    11341 ttaataaacc aacaaagaac tggttaaaaa ccattaataa accatttgat aattgctccg
    11401 tatcggtagt cacacgacta atcaagtcac ctatgccacg tttatcaagg taagctatag
    11461 gtaatagatt caacttttcc attacagcct ttcttaaaga agcaacataa tgaaaaatta
    11521 agcggttata gagcagagga ttaatccatt gaataatagt atttgctaaa ataactgcaa
    11581 ccatcttcca cattataggt aacaataata tcatagaatg aggcgataat acaacgtcta
    11641 ccgcctgtcc aattagaaca ggcaaataaa cagtgaggca aacttgtact accgtcccta
    11701 ataatgccat tcctactaag ctttttttag aaagaagatc ttgaacaagc tttttttgta
    11761 tctttttagg atgacttgct ttcatttact cctcctcctt aaaattttgt gaatggtaaa
    11821 tggacttata aactttgtta tgagctaata aagattgatg attatcaaaa ccaacttgtt
    11881 tgcccttatc aagaagtaag attctatctg cattttgtat actattaatt ctctgtgata
    11941 caataataag attagtttgg ttgaaatgtt taattatagc tttaaataaa cgagcttctg
    12001 ttaaataatc caatgcagat gttgcatcgt ctaaaatcaa aaatggaatc ttatcttgaa
    12061 ctaaagctct tgcaatcgtc aacctttgcc tttgaccacc agagaaattc ctaccaaaac
    12121 tctctacagg ggcatcaagt tgaccatctt tttcttttac aaaatcacta gcttgtgcta
    12181 tttctaaagc agtccaaagt ttttcctcac taactttacc aagtcccaaa gaaaggttag
    12241 aacgaatagt tcctttaaac aactgagcat tttgaggaac aactcttacc aggctacgcc
    12301 aatttgaaat tgtatctgga ctttttccct gatgatatat atcaatatcc ccttcttgca
    12361 ctttataaat atgaagcagt aaattaacca aggttgattt cccagagcca gttccaccaa
    12421 ttatccctaa agtttctcct acattcatag aaaaagtaat atcagataaa gctttttcag
    12481 aggagtttgg ataagaaaat gccatttctt gcacttctaa agccttattc gaatattttg
    12541 gctcaagttt gtcatcaatt atttcagacg gtctttcaaa aacggctata attcgcttag
    12601 cactgatata actttggttc agtgatgtca ccaacattgt catttttaac aattcaacaa
    12661 gaatttgcaa taagtaatta attaaagcaa ccaacattcc ttgtgataaa agatgattag
    12721 ctatatttaa attaccacgc caaataatca caaccaaaat gatattaaca actagaaatg
    12781 ttagaggcgt tactaaacta gaaagcctac ctgcttttaa ttgtaaattc gtatagtgat
    12841 aattgatatc attaaatgct tcactctctc tatctacttg attaaaggca cggataactc
    12901 gtacaccttg aagttgttgc ctagtcaact ttaccaaata atcggttaaa gttctgattt
    12961 taaggtaaat aggatttaat agtcgagaca ttacaaaaac aatgataaat aacgtgacaa
    13021 ccattatcaa gaaccaaata gtcaatgagg gactaatgga aaaagccata attatggcac
    13081 caaaaacaat aatcggagcc ctcaaaaata aacgtaaaaa ttgatttaaa ccagtttgga
    13141 tttgaaatgt atcagcagtc aaacgagtaa tcaaactagc agttcccaat tcatctctgt
    13201 cttttttacc cagtgacatg actttttgat agagatcctc tgtcaattgt ctcgtataac
    13261 caactgcggc ttttgaagaa taatattgag cagttatcgc tactacaaca cccaccgcag
    13321 ccaaaaagaa aatcaatcca atttgcaaca gtaaactact tctatcccct cgtggaatgg
    13381 tctcgtcaat catttttgca ataacgattg gtaccaaaag ttcaaaagag gcttctaata
    13441 atttgaaaag gggtccaaag atagtttctt tgatataccc cttaaaataa taagttagtc
    13501 ttttcatact tacccttctt aaaacaactc cattattttt atcaaataaa ggttgaattg
    13561 aacttcacgt gagtagtaaa tattaccacc atttttatag agcttccctc ctgaaattaa
    13621 ggcgagagga tgatagtaaa agtaagtgtc cccgttataa tttcctaggc tgggagctac
    13681 aacattttta gagtaatttt tggccaactc aatggaattt tcacccccat gatccgctac
    13741 ataatcaata taagaagaac caaaattata tgcttggaca gcagtccata catcaacctt
    13801 agctttttta gcttgactta tattttgtga caacagagtg acgccatgtt taatactggt
    13861 gtgactatcc gtaatactgt tcgtagttcc actgatactt tcacttgatt gcatcacatc
    13921 tatagcatct cccttagttt cagtataaat catagataat actaagtaca cattagcctc
    13981 tgaaccattc tctgcaagag tattctgcac taaatcatga tattgaagca cttttttaac
    14041 attttcatgg atgataacta gacggtaccc aatgaaaata ataataatta atgctatcaa
    14101 acgttttaaa aatttaaaca tattatttat tttgaatatc ctgtatgttt ttgattagta
    14161 ttgagtatgt accatcaggg ttttggataa attcaactga ttcggcatct tggtagacat
    14221 tgttgggtac aatcaattca ataccatttg aaagagatag cttctgattc tctaacttct
    14281 taatttgacg ggaatgatca atatcactta cttgaacaga tccaggtatt gcgtctttca
    14341 attcatcaac aaaagtcagc ctggctgtta aattatcatc aaataattga tctgctaatt
    14401 tttctggaga caattcttgc tcttcttcta aattcttata aatagccgat ttcatttttg
    14461 attggaaaga aaaatcatct ttgttgaaat tttcagcaat tttttgagct gtctgttcaa
    14521 ccatttttat agatttttta acagactgtt ctggttgtac ttgtaataaa ttttcagaaa
    14581 aataattagc aaatgaacca ttgtgtttga tacgcttttc aattaaatag tattgctttg
    14641 atgacttgtt aacaaccaaa gcttcatctg gagtttgagc agcacttgga aggttattct
    14701 gtgttattgt aattggctgc tcttgatttt cagaaacatg agcaaattgt tcctttaatg
    14761 agattcgaag gaaagcaaaa tgttccattc catccttgtc aaattgaata aaaactaagt
    14821 cattcgtttt ttgatcttcc gatatgacaa actcttcttt ccaaagttgc gcaatttgac
    14881 aggaagaaac atataaatca tcttgtaaat gactcataaa tacattatcc tctccaaaat
    14941 agccacgctt agcttcatca gaaaaaactt tacttaattt tttacgaaag tagtcatcaa
    15001 tccttggagt tagggttaac ggagtatctg atagcactaa ctctgtgtcg ttaggagaaa
    15061 actgatgtat aataatctgt ttgatataaa aatccatcat aacattaatt ctcatacaaa
    15121 gggaaagcgt ccgttaatga tttaatttct tgacgaactt ctgtgaggac atcttgattt
    15181 tcatgatttt tgagtgcctt aatcattaat tcagcgattc ttcgagattc ttcaactccc
    15241 attcctcgac tagtaattgc tggagtacca attcgaatac ctgatgtttt aaatggtgat
    15301 agtcgctcaa acggtataga gttcttgtta agggtgatat tgacttcttc aagaacattt
    15361 tgtgcctttt tgccattctc aataactttt gtgacgtcta ctaagaacaa atgattatca
    15421 gtaccatcag aaatcaaatg aaagtcatca tcttctttaa aaactttagc catggcttga
    15481 gcatttttaa taatatcctc accgtatatt ttaaaggaag gatctagggc ttctttcaaa
    15541 gctacagctt ttgcagcaat tacatgttct aatggtccac cttggagtcc tggaaagact
    15601 gcagaattaa tttttttagc aattgcttca tcattggtta aaatcaaacc acctctaggt
    15661 ccacgtagag tcttatgtgt tgtcgttgtc gttacatgag cataaggaat aggactaggg
    15721 tgatgagcag aagcaactaa acctgcaatg tgagccatat caaccattaa gtaagcatct
    15781 actgcgtctg ctatttgacg aaatttttca aaatcaataa tgcgagaata agccgacgcc
    15841 cctgcaacaa ttaatttagg ctgcgtttcc tgtgctattt tcaaaatgtt atcatagtct
    15901 agcatctccg tcttaggatc aacagagtaa gaaacaaaat gataagtttt tcctgagaag
    15961 ctaactgaag cgccatgtgt taagtgccca cctgctgcta agtccatccc taagacagta
    16021 tctcccggct cgattaaagc catgtaagct gctgcattag cctgactacc agaatgtggt
    16081 tgaacattag caaactcagc attaaataac gtcttagctc gttctatagc taaactttca
    16141 actacatcaa cacaatctgt tcctccataa taacgatgac taggataccc ttcggcatat
    16201 ttatttgtca aaacagaacc ttgtgctgcc ataacagctt ttgaaacaac gttttctgat
    16261 gcaattaatt caatattatt ttgttggcga atttcctcat catgaatagc ctgccaaagt
    16321 tcttggtcaa attctttaaa attgtcttta tcaaaaatca ttttttactc cttaagctcg
    16381 ttataacttt taaataatgg tttcaatcat tatatttatc acatttttac tattagttgc
    16441 aagatgatta aattaatttt caaaaattaa ttcaggtatc gtagccgtta gtacctcttg
    16501 cgtgattgca ccttgcctta aaatcttaac tctatcacca gacaaatcaa taatagtaga
    16561 atccacacct gtcaatgctt tatcatcttc aatacctaaa acttgatgat taaactgttt
    16621 ttgaatttct gagaacacac gaccactatc acatccagaa atattagcag aaggaccaat
    16681 cagtggacca tggttagcaa tcatatcaag ggttacaggg tgtttaggca tccgaaatcc
    16741 tacgcttaat aacccagaat tgatccaatg cggaacttct tgtgaagctt ccaaaataat
    16801 ggttagcggt cctggtaaaa aagcatcgta caattgcttt aaataggtcg gttgattttt
    16861 agaatatttt aaaatagtct caaaatcaca tatatttaag ttcatagcct tatcacgagg
    16921 ccttttttta agctcataga cgtggttcac agcttcttca ctcaaagctt tggcaaagat
    16981 tccgtagacc gtttctgttg gcataataac tgcgccatga tcctctaaaa tctgtcccaa
    17041 atcattcatt atctaatacc accattctat ctttaccaaa tatatctttc aaaactctgc
    17101 aacgattgtt tggaaaatat cttttcaata aacttcgtaa atcatcacct tgtttgtaac
    17161 ctatttcaaa atataattta cctctaggtt gcaaatattc tcttgagttt tcaatgattt
    17221 tacgataaat agctaatcct tcttcgtctg caaaaagggc actatgaggc tctgaagcta
    17281 gcacattctt ccccacctcg tctttgtcat tgtaagaaat atagggagga ttagacacaa
    17341 taatatcaaa tttacctgaa atgttactaa agacatctga ttctataaaa gtcacttccg
    17401 catcacaatt attagcattt tcctttgcca agtctaatgc agatacagaa atgtcactag
    17461 ctagaacatc ccaagagggg cgttcttttt ttagagaaat ggcaattgct ccactacctg
    17521 taccaatatc taaaacagaa caatcttcta ctttattctc tgataagatt agatcaacca
    17581 actcctctgt ctcaggcctt ggaattaaaa ctcgttcatc aacaaagaaa ataaggtctc
    17641 taaagtaagc tttacctgta atatactgcg gtgatctatg ttttttaagc tgttgaaaaa
    17701 tattttccat taattgctga tcagtatcac tcacactact attttgattt aaaaggtaat
    17761 cagttgttga ccattgtttt aggtctttta atacataaat aaaattttcg acttcttcac
    17821 cacaagcttc taataactgt ccgtaatgct tgattaactg cgcataattc attatttatt
    17881 taaggcttct aacttttgag tctgatcata cataacaagt gcatcaataa cttcatccat
    17941 ttttccagat aaaatagtat ctaatttttg aagtgtcaaa ccaatacgat gatctgttac
    18001 acgattttgt gggaaattat aagtgcgaat acgttctgaa cgatcaccag taccaactgt
    18061 tgatttacgt tcagcatctt gttcatcttg agcaatttgc gcaaaatggt cagcaacacg
    18121 agcacgaatg attttcatcg ctttatcacg gtttttttgc tgagtacgct cttcttgcat
    18181 ttcaactttg attcctgtag gaatgtgtac catacgaact gcagtagcaa ctttattgac
    18241 gttctgacca ccagcaccag acgcgtggta aatatcgacg cgaagatctt tttgatcaat
    18301 ttcgtattct acttcttcaa cttctggcat taccaaaaca gttgcagttg aagtatgaac
    18361 acgaccctga ctctctgtca ccggaacacg ttgaacacga tgagctcctg attcatattt
    18421 aagcttagaa taaactgact gaccagaaac catagcaacc acttctttaa taccaccaac
    18481 accattaacg gatgattcca taacttcaaa acgccatcct tgagtttcag catatttttg
    18541 atacattgtt aaaagatcac cagcaaaaag cgctgcttca tcgccaccag cagcaccacg
    18601 aatttctaaa ataatgtttt tatcatcgtt aggatcttta ggtaatagaa ggatttttaa
    18661 tctttcttca tactcttctt tagctgcttt tgattcttta agttcttctt tagccatttc
    18721 ttccaattca gcatcaccag aagcatcctt gatcatttct tcagcatctg aaatattttg
    18781 aatgacttgc ttatattctc tataagctgt gacagtttct cgagtattag cttcttcacg
    18841 tgataattcc ataaatcgct ttgtatctga aacaacatct ggatcactaa gtaactcacc
    18901 taactcttca taacgatctt caactgcctg caattgatca taaatattca tttttctttg
    18961 tatctccttt aaaaccgatt atcttttaaa tatctggatt aaaataatgt tttcgacaaa
    19021 ctgggatata tgtttcatta ccaccaattt gtatctgatc accttcatag acaggttttc
    19081 cgttttctgt ccttaaaacc atggttgctt tcttactaca gtattggcaa attgttttga
    19141 tttcatcaat cttatcggcc aataatagca aatgttttga accttcaaaa agattgtttt
    19201 ggaaatcatt tttcaaacca aaagccatga ctggtacatc taagtcatct actactctag
    19261 ctaaatcgta gacgtttttc ttactcaaaa attgacattc atcaattaaa acacaataag
    19321 gcttttgtgg taaattctga atataggaga aaatgtccat atcatctgaa attggaactg
    19381 cttcacggcg cattccaata cgacttgaaa cgactccaaa ttcatctcga gtgtccaagg
    19441 cgcttgtcat aataaccaca ggttttcctt gttcttcata gttatgagca acttttaaaa
    19501 tttcaatggt tttaccagaa ttcattgtcc catatttata atagagttga gccaaagcgg
    19561 aaaatcctac tttctatagt attctaccta ttttaccaca aaggctgaag ttttcccagc
    19621 cattgaaaac agtgtgtact ttttcacaac attatgttac aatgatgccc aataacataa
    19681 aggagtaaca ttatgccatt tgtaaaaatt gacttgtttg agggacgctc acaagaacaa
    19741 aaaaatgaat tagctcgcga ggttactgaa gtagtatcac gcattgcaaa agcacctaaa
    19801 gaaaatatcc atgtttttat caacgatatg cctgaaggaa cctactatcc tcaaggtgaa
    19861 cttaagaaaa aataagaaat agtaaaagag gttacccagg taacctcttt tgatagtctt
    19921 tgtaagcata tttcttgaca aattgaaaat atttgaaaat aagtgttgat tttcaagtta
    19981 gaaaacggta tcattagaat atattgcttt ggaggtggaa aatttgcaac tttctcatag
    20041 tttaagactc atgggaacga ctatcgatat tcaaattaat tctaaaaatg ctcaaaaaca
    20101 aatccaagag gtcatagaac tcttggagct ctacaaaaat cgctttagtg caaatgactt
    20161 taattcagag ttaatggcta taaataacaa cgctggtatc aagcctattc aagtacatcc
    20221 agacctcttt gaattaatca caattgggaa agaacacagc ttagctaggc ctagtaacct
    20281 aaatattgct attggtcctt tagtgcaaac ttggagaatt ggattttcag atgctaaact
    20341 acctagccct agtgaaatat cagaagctat gattttatct gacccaactc atatacttct
    20401 agattcaaaa aatcaatctg tttttttaaa tcagatagga atgaaaattg atttgggtgc
    20461 acttgcaaaa ggatatattg ctgacaaaat tatgacctat ctcaaaaatg agatgattga
    20521 ttctgctatc attaatttag gcggaaacgt tttagttcac ggagataatc ctaatcgttc
    20581 agaagggtat tgggttatcg gcattcaaca tccgaaaaag aaacggggac aaaatattgg
    20641 agctgtcaaa attaaaaacc aatctgttgt aacctcaggt acttatgaac gccgtctgat
    20701 tattgacgat aaggaatacc atcatatttt tgatcgacag actggttatc cgatacaaac
    20761 agagatggct agcatttcta ttgtttctaa acaatccgtc gactgtgaaa tctggaccac
    20821 aaggttgttt ggcttatcca ttaaagaggc tcttgatatt ttaaacgctg tttcctacat
    20881 tgaaggcatt atcatcacta aagatgaccg tatctattta tctgatggct taaaacatca
    20941 ttttcaacta ttttatcatt aagaaagagg atttctaata tgaaacttat tggcatcgtt
    21001 ggaactaact caaataaatc aacaaatcgt caattattac aatacatgca acaacacttt
    21061 gctgacaaag cagaaattga actcatcgaa gttaaagacc ttccgttatt taataaacct
    21121 gctgataaaa atgttcccca agtaattttg gatattgctg ctaaaattga agaagctgat
    21181 ggtgttatta ttggtacgcc agaatatgat cattctatac cttcggcctt gatgagtgtt
    21241 ttagcttggc tatcatatgg tatttaccca cttctcaaca aacccgttat gattactggt
    21301 gcttcatatg gaacacttgg atcatcacgt gcacaattac aattacgtca aatacttaac
    21361 gctcctgaac taaaagcaag tgttttacct gacgaattct tgttatcaca ctctctccaa
    21421 gcctttgata aagatggcaa cctacacgat atcgaaacaa gtcagaagct agatgctatc
    21481 tttgatgatt ttcgtctttt tgttaagatt actggtaaat tatcaaatgc tcgtgattta
    21541 cttcaaaaag aagcagaaaa ttttgactgg gaaagtttat aagagtatag aagggaataa
    21601 ctaagatgaa atttgtaggt attgttggct caaatgcaga gcaatcatat aatcgtatgt
    21661 tgcttgaatt tattcgcaag aattttaaaa ccaaatttga attggaagtc cttgaaatcg
    21721 atgatattcc aatgtttaat caagaccaaa attgggaaga aagtttccaa cttcgtcttc
    21781 ttaataataa gatcacacga gctgatggcg ttattattgc cactcctgaa cataatcata
    21841 ctattacggc cgctcttaaa agtgtgcttg agtggctctc atttgctgtg catccactcg
    21901 aaaataaacc tgttatgatt gtcggtgctt catattatga tcaaggcaca tcacgtgctc
    21961 aaatacacct tcgtaaaatc cttgatgctc ctggcgttaa tgcttacaca cttccaggaa
    22021 atgaattctt attaggaaaa gctaaagaag cctttgacga taatggaaat attatcaatc
    22081 ctgggactgt tcaattttta gaaacttgtc ttgataattt tatcaaatat gtaggagttg
    22141 tatcatcatt gagaaaacct aaagctattg aatctgaaga cctctactgt cagggacaaa
    22201 tcgatacaac cattactggt atcgatccag atgatcctga ttggttagac aaggcagctg
    22261 agaaagttaa cgccgttgaa ggggatgctt acgttaaact agacaatggt ttacttactg
    22321 ttaaccaact taatatgttt ttaaatgcta tgccatttga gctaacatat gcagatgata
    22381 ataatcaatt tctatattac aataataatc atccagatcc agatacaatg ttaggaaaac
    22441 gtgttcgtga acaagtagga aaccgtatgg ctacagttca tggtagttta ccaccggcac
    22501 gaatgaaaaa tgttgaatgg gttatcggta ctcttcgtaa tggaaatcaa gaatatgtac
    22561 gtacagtcgt tcctggtaca cctccagaag ttatcaatac tcacaactat caagctatgt
    22621 actacccaga cggatcttat gctggtatta atgaaattat ctttaacttt aaaccgtggt
    22681 tagattggta cctccaaact actggacaac gtttggtcgg tggaactact gctgctcctg
    22741 gagcttcagc agttgacgct acttctagcg cttcaactag cacaggggat gctcccgtgg
    22801 atcaagccac agatgccact tctggtgcct ctgaacatta atctcctcgc ttttgaggat
    22861 ctgatacctt aaaaagatat caagatcctc ctttttaata tttaatcaag ttaataaaat
    22921 cacataaaag gaatatacta tgtcactttt tcaagaaaaa attgcttaca attgcgccaa
    22981 aaaggaagcg ctttacaaag agagtttagg acgctacgcc ttgaggtcaa tgctagcagg
    23041 ggcttatttg acaatgagta ctgctgctgg tatcgtcgca gctgatacta ttggtaaaat
    23101 ttctcctgct ctatcaggtt ttgtatttgc tttcatcttt agttttggac ttatttatgt
    23161 tttaatattt aatggtgaat tggcgacatc taatatgctt tatctcactg caggagccta
    23221 taataaaaat atctcttgga aaaaagccat gacaatttta atttattgta cttttttcaa
    23281 cctcgttggt gcttgcatat tagcttggtt gtttaatcaa tcgtactcat ttcagcattt
    23341 aacaaatgat agctttttag ggcatgtcgt tgctaaaaag ttaagaaaac ctagttcagg
    23401 agctttccta gaaggtatta ttgctaatat gtttgtgaat ttagctatcc ttgcttacat
    23461 gttattaaaa gaagaatcgg caaaaatgac ggttatttta tctgctattt ttatgtttgt
    23521 ttttctatca aacgaacact taattgctaa ttttgcatcc tttatgttag ctgcttttag
    23581 ccatatcgaa catattaaag gttttactct tctaaacgtt atccgccaat ggacacttgt
    23641 attctttggt aactggatag gagggggcgt tttcatcggc ctagcctatg cttggttaaa
    23701 taaaacaaaa acttctcacc atgaataaaa gaaaaactga gtaataaatt actcagtttt
    23761 tcttttctat tatcatcttg aaatcaataa ctaaaaatta acttttttga taaataaagg
    23821 aaagtatcat attaattttt taggcaatcc attcaaaatg atatttaaaa caacagctgt
    23881 cagggttgag ataacaatac catttgtcaa aaacatttgt aaagtattag gaagactaat
    23941 gaagagattt gttccattaa aacctactcc tgctgcaatt gatacggctg caatgataaa
    24001 gttatgctca ttatgctcaa aatcaacctg attaagcatt ttcatccctt gaagtgctac
    24061 cataccaaat aaaactaaca tagcaccacc aagaactgga cttggaatca tttgagccat
    24121 tgcgccaaat ttaggtaaca agccaagtat aactaagaag agagctgtaa aatatatcgg
    24181 cttacgcgta cgtatcccag atatctgtac caatcctaca ttttgagaaa aaccggtata
    24241 tggaaaggta ttaaataagc cgccaagtaa tactgccaat ccttctgaac ggtaaccatt
    24301 acgaagtctc ttactgtcta atttatcgtt tgtaatatct gaaagcgcaa ggtaaacacc
    24361 tgttgattct accatagaaa ctgttgcaat aatacacatc attaaaatag aagtaaattc
    24421 aaatctagga gctccaaaat aaaatggctt cggaatatgt acaagtggtg cttctgctac
    24481 cacagaagca tcaactaagc ccataaatgc tgcaagaatt gtacctgaaa tgagtccaat
    24541 aaggattgaa attgacttca aaaagccttt tgcgaaaata ttaattaata aaacaacacc
    24601 aatcgttact agtgataaag ttaaagattg taagcttggt tcttttgcat tatcacccat
    24661 atttcccatc gcaactggta ttaatgtcaa accaattgta gtaataactg atcctgttac
    24721 aattggagga aagaaatttg caactttaga aaaaatgcca gcaactaaaa cgacatatat
    24781 tcctgaagca attaaagctc caaacatata gccagaacct tgttgtgcac caataattga
    24841 taaaggagct acagactgaa aggcacaccc taatacaact ggaaggccca ctccaaagtg
    24901 cttacttaat cgtaattgta ataaggtggc aatcccacac ataaaaatat ctgtcgcaat
    24961 aagataagtt agttgcttag cattataacc aagagcacta gcaatcatga taggtacaag
    25021 aatggatcct gcatacatag ctaacaggtg ttgcaagcct agaagtgctg cttgcgaatt
    25081 actttgctca ttaaatttca ttaagcatca gcctccgcaa aaacaactcg accattttca
    25141 aacgccttaa tacgagcaag agaagtaact ggaacacctg ttttttctaa caaatcacgc
    25201 ccatcttgga aagatttttc aataacgatt ccgataccag caaccttagc tccagcttga
    25261 ccaataattt caagtaatcc tttagccgct tgaccgtttg ctaaaaagtc atcaatgatg
    25321 agtacagtat catcgttaga taaaaagcga ctcacaatag aaacttgact cgtaacttgc
    25381 tttgtaaaag aatacacttc agcagttaag ataccttcag tcatagtaat gttcttagct
    25441 tttttagcaa atatcattgg tacgcccaat gcttgagctg cgtacactgc tggcgcaatt
    25501 ccagatgctt caatcgtaac aaccttcgta atgccggctt ctttatattt atcagcaaaa
    25561 actttaccta tttcctgcat taactcaaaa tctacctgat gagtcaaaaa ggaatcaacc
    25621 tttaaaatgt tctcacctaa aacatcacca tctttaagaa tacgttcttc caataacttc
    25681 ataataaccc cttcactctt tagaaaataa aaaaataact tacctcaatc agaggacaca
    25741 aatctcctct aatttatcgc ttcattaaca tgaaccaaca ggcaagctat ttattagctt
    25801 acttattgtt agacattacg gtgtcttgta gagacgctaa gccaatcctc agcataaata
    25861 agtaaatcat atatttaatt gttatgtatg atagataact attgtaacat ttttaattaa
    25921 gatttcaaga tttaatttaa atggaatcac cattccaaat agaattttta acaataacgt
    25981 aatcaacgtg tctcaagcta tccaattctt ttccacctgc gtaagaaata gaactttgca
    26041 aatcttgttg catttctgtc aaagtatcct ctagtcgccc tttaacaggt aatagaattt
    26101 tcttaccttc aacattttta tgttctccct tttgatattc agaagctgag ccatagtatt
    26161 ctttaaattg ctgtccttct acttccacaa gttttccagg actttcaagg tgaccagcaa
    26221 acagcgaacc aatcattacc atactagcac caaagcggat agattttgca atatccccat
    26281 gtgttcgaat accaccatca gcaataattg gcttgcgagc agctttagaa caccaacgta
    26341 aggcagctaa ttgccaacct ccagtaccaa aacctgtctt gactttagta atacaaactt
    26401 taccaggtcc aataccaact ttagttgcat ctgcacctgc attttccaat tctctaacag
    26461 cttcaggcgt tcctacattt ccagcaataa cgaaagttcc tggaagctct tgtttaatgt
    26521 gttggatcat ctctataaca ctgttagaat ggccatgtgc aatatcaata gtgataaact
    26581 caggagcatc ttctttcaga cttgtcacaa aatcatattc atagtctttg accccaaccg
    26641 aaatcgatgc aattaaacct ttatcatgca tacgcttgat aaaaggtttt ctttcttctt
    26701 cattaaaacg gtgcataata taaaaataac cttcacacgc taatgtttca gctacctctt
    26761 catcaataat agtctgcata ttagcaggga ttactggaag tttaaaggta tagtttccaa
    26821 gtttgacact tgtgtcagct tgtgagcggc tagatataat acacttgttt ggtattaatt
    26881 gaatatcttc ataatcaaaa acaggaatat cgttaaacat aatggttctc ctaatgagta
    26941 aaatggcctt cctataactt tgtataacaa agcatcgcta ggtaaaaaca tacctttcct
    27001 ttttaaatta tagactttat ttatttttag tcaatttttt ctcagtttat ttgaataatg
    27061 ttcgtttttt gcaaaagaaa ttaaaatcct atagagcagt actctatagg attttaattt
    27121 attatattgc ttttcttttt cgaagataag ttaatcctac aatagctaag ataacaacaa
    27181 cgataacgaa tagaactgca aattgactac caattatagc tccatttttc ggaatagcta
    27241 cttgtccgat ggtaaaaatt tgtgaggcta ctgcggttgc aaaagctccc gcaaattgtt
    27301 gtagtgtatt caagatacta ttaccatccg ctgattgtgt tggctttagt tttgttaaag
    27361 cagtcgtaac catattactt gctccaatcc ccataccaat tttagtaaga atatcaaagg
    27421 ctaaaagtac tacaacagac ttcgcaggaa tgaatatagc tgtaccaatt aaacctatca
    27481 aagaaaggga gatacctatc ataattggct taaatgcacc aattttatcc aggagataac
    27541 ctgcaaaagg ggataaaaaa actactgcta ttgcacctgg aaacataaac aaaccagctt
    27601 tggttgaagt ttgatgtaaa acaagttgca agaggttagg taataagaac gaaagtgcta
    27661 gaggaatcat ttgaaaagcc agtacgccat acaataagat tgagaaatct ttatctttaa
    27721 aaacattcaa attcaaaaat tctagctgtt tatttaaata gtaaaaaata acaaaactaa
    27781 caatagctac taataagtaa aaaatagaca ccttttcaat agctaaaaga agggttgcca
    27841 agccaatacc taaactgaca aaggcaggaa aattgagttt atcattaatt cttacttgac
    27901 gtttgggaat agaagccaaa cctataaagg tggatatgat aagtattggt gccagaagca
    27961 taaaaatcat cttccatcct aacattcctg aaataacacc gccataagta ggtccaactg
    28021 ccggtgctaa aagtgtgacc atagcagcta ctcccatcat tagtcctcta cgctccatcg
    28081 ggatttgtgt aagaatgata tgaaacaata aagggagggc taatcctgtc ccaacacctt
    28141 gcaagactct ccctactaac aatatagcta aattaggggc aaagctatca attaaaacac
    28201 cagataaaaa aagcacattt gccaatgtaa aaatttgtct ctctgataaa tttttaatca
    28261 taaatgctga caaaggcaca gtcacagcaa cagctaacaa atttcctgtt gtcacccatt
    28321 gaataacagc ggggttaacc ccaaattctt tcatcaaaag cggaaaagtg acattcatag
    28381 atgtttcaat aagaatccct gaaaaaccta atatagccgt tgtaattatg gcaggcataa
    28441 tacgccatcc ctttttagtc attaaaatct ccttgctttg attattatca taaaatagag
    28501 caccccgcta aatcaaggtg ctccatttaa ttcattcgaa ttaaaattaa attgtttata
    28561 ttagttgtca aaggacaatc aacagctctt attatatcaa ataaagctaa gaaaaacaaa
    28621 gagttttttg aatattaatg tcttcacttt cttatctatt ctctacccat caatcaccac
    28681 ttcagagaac ataacaattg caatttactc ttaataccgt tatcatgttc atatgctaat
    28741 acccaagaaa atagtggctt ttgcctcaaa tgaatctgaa aataatttct ttattacgtt
    28801 aaagacttac tttaattatt tgttttctat acaaatcatt acagatattt caactctcaa
    28861 tcatgcagat tttgacggta gctttgcatt ccatgatata gaaactagca tacctcatct
    28921 ggtaattgac tccaactact tagcgattag tcagactaac tcaaaaacag aggctaacga
    28981 tattaaaacg ttctcggaac tctccaaaac aatgaccgag tttcactata tgctaaattt
    29041 tgacttattt aaccatctcc catatcgttt tagattgcat aataaggacg gtcaaacaat
    29101 ttattcgaac cataaacctg aagatccttt tgatatttat cctgaagaag aatatcctat
    29161 agacaaatgg gttcaaaaca gcctaataga gaaaaaagcg aaagagctac atttactctt
    29221 acctagcgca tcacaagatt atattctagt acaatcttat aaacgcttag aaaatgatag
    29281 tgggcaactc gttggttata ttgaacatgt tcataatatt aaaccactgc tagagggtta
    29341 tttaaaagaa tccggtcaag ctatcgttgg atggtctgat gtcacgtctg gtgcttctat
    29401 ctctaacgat gattttgaac tataatccac tcgtcactct aaaaaagaaa actaataact
    29461 aaaaggcact ggtttaattc cagcgccttt tattaatcaa tctccagttg ttgtaagttc
    29521 aattttttct ttaattggta gacctcgatt agctgtaaaa atcaaaggaa ctacttctgt
    29581 tgtaacttcg gaaaattgta gaccaaacca tcgtattggt gttgctgtcc aatgctttat
    29641 agatgatttc attagcataa caaatcgttc accaggtttt aggttttgac tacttgagaa
    29701 acgttcatca aggacaataa acctaaaatc tcctacttta ctatcaggtc taacgctgta
    29761 tgtctttcct tgtttaggca gacgtccact ttcaagtaat tcttctacaa tcgtacgaag
    29821 atagcgtgac acagtctgac gcatcttaaa tccaaggtaa agttctacct tcacaatgaa
    29881 gtctgttccc atcatgtcaa ctttatactc agctgtataa ggttcatcag tgaccttaat
    29941 gtttacaaac caataaactt gtgctttttt agggcgctta tcaaggatag aatacatgat
    30001 agacttatca atcatgtctc cttccatacg atttgtcaaa taaacaacgt tagtatgata
    30061 aatcggataa cgatgatcat gtctaagttt atctaattgt ccgatgtaat cttttaaatc
    30121 caacaactta acataacgcg aaacaatttt actaccttta taccagatag tcatgatgaa
    30181 tataattgct actgctataa taacaacaac atagcctcca tgcataaatt taacagctga
    30241 agcaaggaag aagattccct ctaaaatacc aaagaaaatc atcatcaata agactaaacc
    30301 tctttttact cctttttgaa ttaaaaagaa agacagtaaa atagttgtca ttagcatcgt
    30361 aattgttatc gctaatccat atgctgcttc catgtgcgct gaagtcttaa aaagcaaaac
    30421 aatagaggtt gtaatggcaa ataagaacca attaataaca ggtatgtagg tttgaccaat
    30481 attgtcacca ggataagttg aacgaaattg tgggaatatt tttagtcgca tagcttcaga
    30541 aactaaggta aatgatccag aaatcagtgc ctgtgaagcg atgatagctg ccaaagtagc
    30601 taaaataacg acatgcattg taaattgcga aggaatacta gcaaaaaagg gattcaattc
    30661 atttcctgcg ttcttattag ctaaaatcca tgccccttgc ccacaataag aaagtataat
    30721 ggcaacctta acaaacggcc atgaaacatg tatatttcca cgcccaacat gacctaagtc
    30781 agagtatagt gcttctgccc ccgttgtcgc caggaaaata gatcctaaaa taaaaatacc
    30841 tttatgattc tctggactaa ataacaattt taaaccgtag tatggattaa ttgctttgaa
    30901 aacttctgga tgggcaaaac tattaaggag accgctaata cctaaaaagg caaaccatat
    30961 aaacataatt ggcccaaata atttcccaat aacacccgtt ccaaaccttt ggatggcaaa
    31021 aagtaacagt aaaataaaca aggtcgcaaa aataacgtta ctctgattct ggaaaatatg
    31081 ctgcaaacta ggaactactt ttaatccttc aacggctgaa gttacggtta cagctggcgt
    31141 caaagctcca tctgacaaca aggttgcacc tccaataaca gccggcacaa ttaaccaagg
    31201 tgtcattttt ctaacaaggg tatataaaga aaaaatacca ccttcgtgat gattatccgc
    31261 ctttaaagct acaagcacat acttgatagt tgtaataagt gtcaaggtcc atatgattaa
    31321 agatatagaa cctaagataa acgattctgt gacactagaa ataccacctt ggttttcaac
    31381 caatgattgc atcgtataga gtgggcttgt accaatatct ccataaacaa tgcctaaagc
    31441 aataataaat cctgcttttg atgctttatc aaaagaagaa tgattgacat gttgcatttc
    31501 tgccaaccct ccgattaaat aatattagtc aaaaaaatgg ctgaactcat tatagcagat
    31561 taagttaaaa aaacaacact tatctataaa aagaccagaa attattaaag cgtttcctgg
    31621 tctttactat tttaatcacg ataaacaggt tgaggcccat aagtttgact gactggcatt
    31681 atttctatgc ggttaatatt aatgtgttct ggttggctag caacccatga cacggtatta
    31741 gcaatatcct ctgcttgaat tgcatgcgca ccttcataaa gtttttcaac tcttttatga
    31801 tctcctttga aacgtacagt tgaaaattct gtcccttcac ataaaccggg ttctaggtta
    31861 gtaactctga ttttagttcc agctaaatcg gcacgcaagt tgagagaaaa ttgttttaca
    31921 aaagcttttg atgcgccata gacattagct cctggataag gaattgttcc tgcagttgat
    31981 cccaaattga taattaaccc tctattaact tctaccattt tcggtaaaat gcatcgtgtc
    32041 aagtaaatta atcctacaac gttagtatta atcatagtca tccaatctcc aaaatcagct
    32101 tcatatgatt tatccaatcc aagtgctaag cctgcattat taaccaacaa atcaattgat
    32161 tgcaaatttt taggcagact ttctaaagct ttgtcaaccg attctctact tgtaatatcc
    32221 atttgaagtg gataaaagta accctctgaa aacttttctc caaggcattt taatttatcc
    32281 attcgacgag cacaaccaat aacgctataa ccatctgaaa ctaatttagt aacaatagct
    32341 gcaccaaaac cagcagatgc ccccgtcaca agcgcaactt tacgtgatat catagacact
    32401 cctcctctag tatatagaaa aacgagctag gaaaaatcct tctcgtttta taacttattt
    32461 tgatttctca tcaatagctt gagcagctgt aatgatagca agtttgtaaa tatcttcagc
    32521 acttgatcca cgtgataaat catttactgg agcattcaat ccttgtaaga tcggtccgat
    32581 tgcttcaaac atacccaaac gttgtgcaat tttatagcca atatttcctg attgaaggtc
    32641 tgggaaaata aaaacatttg cttttccagc aacatcacta ttaggagctt taatttctgc
    32701 agtttccgga acaaaagctg catcaaattg taattctcca tcaacagcta attcgggaga
    32761 taaatctttg gcaattttag ctgcttcttg aactttttca gcttgaggcg cttttgcact
    32821 tccttttgtt gaaaaactaa gcatagcaat cttgggatca atatcaaata ttttagctgt
    32881 atccgcagta tttactgcaa tttctgctag ctcttgtgca ttaggatcaa tgttaatagc
    32941 acagtcagca aaaatataac gttcttgtgt attttcacgg ttcatcaaga atacacctga
    33001 agtgcgtgaa attcctggtt ttgttttaat aatttgaaga gcaggacgta cagtatcagc
    33061 tgttgaatga atggcccctg ataccatacc atctgccaaa ccaagtttta ctaacataac
    33121 cccaaaatag ttaacatcct tcaaaagacg atctgcatct tccatagtag ctttgccttt
    33181 acgaatctca acaaaagctt ctttcatctc ttcaaatttt tcgtattgtt cagggtcaat
    33241 aacgctataa tcttgatcaa caaaaccaag tcttgccaaa gaatcgtgaa cttcgtcagc
    33301 cttacctaaa ataacaggat caacaagacc ttcaaatttc aaacgagctg ctgcacgtaa
    33361 tacacgttca tcgcttccct cagggaaaac aatcttaaca taatcacttg caactttttc
    33421 tcttaaacca ccaaataagg aacgcatata tataaaacct tctacttcta aattaatgta
    33481 attccatgat aacgctgtca aagtcatctg tcaagtctag cgtctgttta tttatttttc
    33541 ccataaaagg gtcgtaaaaa gacaaagaat gacaatgcaa tgcttgcctg tttattccta
    33601 gatccatacg tcctccgtac aaatcatctc ccaaaagagg aaagccgata tgagcaaagt
    33661 gaactctaat ttgatgagta cgccctgtat gtaatttgat atccactaat ctaacatcac
    33721 cataacgagc aactacttga taactagtat gtgcatattt gcctgattca tggacgcgcc
    33781 tcgtaataat actatcaaca tctcgtgcaa taggagctat aatatcacca ctatctgcta
    33841 aatcaccact ccctgatact aatgcataat accttttttc aattgcttta gcctgaagtt
    33901 gtttatctag acgagcatgg gcataaccat gcttggcgaa taacattaat ccacttgtat
    33961 ctcgatccaa ccgggtcaca atatggacct gctgattggc atagttatta gacacataat
    34021 agtgttttat aaaattagca attgtatttg aatgaataga gctaggaata ctagcgaaac
    34081 caaaaggttt atttaaaatc aaaaaatgat catcctcata aacaatatct aaatcgtgag
    34141 gcactggttc caaagtttcg tgagaaggct cgtcaggaat atctatcgta acaacatcgc
    34201 caatctctaa taagaaaata gcgttctgct cttcaccatt gacaaaaatc ttaccacctc
    34261 tgtacttaat ctttgctaat aacccccttg aaacatcatg acttttcaaa agtgttttaa
    34321 ctttgcacct ttcttctgct atgtattcaa atttcaatgt tcaggttctc cgataaaagc
    34381 atcgttaaca cgttcccaaa agctagtgtg acttggagtt gaaacaaagt taatactttt
    34441 ttcgtcaata ctatattcta ttttggtaac atttttgtaa tggactgttt tgttatctat
    34501 agaaattgtg tagacaccaa ctcgtttagg gactatctct atagcatctt ttttggggat
    34561 aataaccgat gaccccaagg tacgatagac gcgattatta agactagaaa tttctgttag
    34621 ttgcaaggct tctatcgttg ggtggagtac tgccccccct agtgatttat tgtaagcagt
    34681 actaccggtt ggtgtagaaa ctaaaatgcc atctccccta aacctttcaa ataccacttg
    34741 attaataaca acatccgcta ccattgtttt ttcaatacgc ttaatagtag actcatttaa
    34801 agcacgcgca cgaataattc gaccatcttc aagggtaata gttactttca aaatgggata
    34861 ggagatttgt tcacctttat catttttcaa attattaatt aatgtgtcaa cttcaaaatc
    34921 tcgatagtct gtataaaatc ctaaatgtcc tgtatgaaca ccgacaaacc ttactttatc
    34981 cagttgtttt tcgtacatgt ggaaagctga caaaagcata ccatctccac caatagaaat
    35041 gacaatatca ggatcctttt ttgataaata aaaatcagga tcatgtttaa aagcagcgaa
    35101 gagctttgag gctacacgct tgctttgata ttttccgtta gcaattatag caacacgtgt
    35161 tgctctatct gtaaaattca tctgtgtcat cactatttcc tacaccatcg ctcaattttc
    35221 gatttaaagg atcaaataaa agctgtgctt cacgaatatc ttcgcgaatc ttacgcattt
    35281 cttcatccaa ttctaaagcg atttttgctg ttttctccag tctttgttta atttcttcag
    35341 ggaagtcccc ctgatatttg taatttaatg aatgttcgat ggttgcccaa aaattcatag
    35401 ccagagttct gatttgtatt tctgctaaaa ctttcttttg tccatctatt gtgtctacag
    35461 gatactctac aacgacatga tatgaacgat atccactaga tttcatatgg gtaatatagt
    35521 ctcgctcctg aaccactgtc atgtcatgac gttttctcaa taaggcaagg acttcatcaa
    35581 cgtcatctac aaattgtacc atgatacgaa gacctgcaat atcttgcaag tcctgagcaa
    35641 gattttcttc tgaaatacca cgtaaaacca tcttttcttg aatactttca accgacttta
    35701 cgcgtccagt aacaaattct attggagagt gacgattttg ctttctaaat tgtttacgaa
    35761 taccacgaag ctttattttt aattcaccaa ccgtttgaat ataagggtct agaaatgttt
    35821 cccaatccat tgacattcta atcctccaaa atatttaatg tactctcttg taaaataaac
    35881 tttttttcta taaaattaac acaacaatta atattatacc atatttagga aaaggaagtg
    35941 ataagatgac acacctagaa attgaataca aaactctatt gaataaagac gaatttaatc
    36001 gtctaacaag cttattttct catgttcaac ctattactca aaccaattac tattttgaca
    36061 cagaaacatt tgaaatgaaa gcacaccgaa tgtcacttcg cattagaacg ttacctaacc
    36121 gtgccgaact aacactaaaa atccctagag aagtaggaaa tttagagcac aatcatgacc
    36181 ttactttaga agaagctaaa tatatcgtta aaaatggcca gttccctgaa gatactgaga
    36241 ttgcttcttt aatccttgaa aagggagttg atccaaccaa attagctgtc tttggacaac
    36301 ttacaacaac aagacgagag atggaaactt caatcggctt aatggctcta gacagcaata
    36361 tatatgctga tattaaagat tatgaacttg agttagaagt caaacaacct aaacaaggga
    36421 aaagagattt cgaccagttt ttaaaagaaa ataatattaa ttttaaatac gccaaaagta
    36481 aggtggctag attctctgct actttaaaga ataactaacg ttgagagatt ggattttcca
    36541 atctctttta taattctcat ttaagcaaga acttaaaatc tcatactatc tctcctcaac
    36601 attgttattg caagcatttt catgtttctt atcgtgaatt ttttctattt ttttggtaaa
    36661 ctattagtat caatctaaag gagtcatcta tctcatggca gaacaatacg ccgataaaca
    36721 aatcaaactt ttctcattaa ccgctaatag agaaattgct gaaaaaattt cccaagcttc
    36781 aggaattcct ttgggtaaga tgtcatcacg tcaattctct gatggcgaaa ttatgattaa
    36841 tattgaggaa acggtgcgcg gtgacgatat ctatatcatc caatctacaa gcttcccagt
    36901 caatgataat ctctgggaat tactcattat gattgacgct tgcaaacgtg caagtgctaa
    36961 tactgttaac atcgtagtcc cttattttgg ttactctcgt caggaccgca tcgctgcatc
    37021 tcgtgaacct attactgcaa aattggtcgc taatatgtta gttaaagctg gagttgatcg
    37081 agttcttacc ctcgacttac atgcagtaca agtacaaggg ttcttcgata tcccagttga
    37141 caatcttttt actgtcccac tatttgctga acactataat caactcggtc tctctggtga
    37201 agatgtcgtc gttgttagcc ctaaaaattc tggaatcaag cgtgctcgta gtttggcaga
    37261 ataccttgac tctccaattg ctatcattga ttatgcacaa gatgattctg aacgtgaaga
    37321 aggctatatt attggtgagg tagaaggtaa gaaagctatt attattgatg atattttaaa
    37381 tacaggaaaa acctttgccg aagctgcaaa aattttagaa cgtggtggag caactgagat
    37441 ttatgctgtt gctagtcatg ggctttttgc tggcggcgca gctgatattt tagagtctgc
    37501 accaattaga gaaattatcg taacagactc tgttttatct aaagagcgta ttcctagcaa
    37561 tattaaatat ctgacagcta gccatctcat tgcagatgct attatccgta tccatgaaag
    37621 aaaaccttta agtccattat tttcatatcg ttcagataag aaggattaat tgatgattta
    37681 tttagacaat gctgctacta ccgctctaac cccatctgtt attgagaaaa tgaccaatgt
    37741 catgacaagt aactatggta atccatctag tatacatacc tttgggcgtc aagcaaatca
    37801 acttttacgt gaatgtcgac aaattattgc tgaatatcta aatgttaatt cacgtgaaat
    37861 tattttcact tctgggggaa ctgagagcaa caatacagct atcaaagggt atgctcttgc
    37921 aaatcagcta aaaggtaaac atattattac ctctgaaatt gaacatcatt cagtcctaca
    37981 tactatgact tacttatcag agcgatttgg ttttgatatt acttacttaa aaccaaacca
    38041 tggacaaatt actgcaaaag acgttcaaga agctttacga gatgatacta ttatggtatc
    38101 tctcatgttt gctaataatg aaaccgggga ctttttacca attcaagaga ttggtcagct
    38161 tctcaggaac caccaagctg tttttcacgt tgatgccgtt caagtcttta gcaaaatgga
    38221 acttgatcct cattctttag gaattgactt tttagctgct tctgcccata aatttcacgg
    38281 tccaaaaggt gttgggatac tttactgtgc tccccatcac tttgatagtc tacttcatgg
    38341 tggagaccaa gaggaaaaaa ggcgtgcttc aactgaaaat ataattggta ttgctggaat
    38401 gtctcaagct cttactgatg ctacgactaa cacccttaaa aattggactc acattagtca
    38461 gctgagaacg acctttttag atgctatttc agaccttgac ttctatctta ataacggtca
    38521 agactgctta cctcatgtac ttaatatagg ttttcctgga cagaataatg gcttgttatt
    38581 gacacaatta gatttagctg gattcgcagt ttcaacaggt tctgcatgta ctgcaggaac
    38641 agtcgaacct agccatgtct taacaagctt gtacggagcc aactcaccac gtctaaatga
    38701 atcaatacgt attagttttt cagaactaaa tacccaagaa gaaattctta aattagctaa
    38761 aaccttaaga aaaattatag gagattaata tggcatttga aaaagaaata aatcttccag
    38821 actgcaatta catttatact atcagcccga acattaaaaa atatacctta cgcgatacta
    38881 cattcactca aaatcgctt
//
LOCUS       DATRTN010000011        35918 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.011, whole genome shotgun sequence.
ACCESSION   DATRTN010000011 DATRTN010000000
VERSION     DATRTN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35918
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.011"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(27..1052)
                     /locus_tag="VBE22_000598"
     CDS             complement(27..1052)
                     /locus_tag="VBE22_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEO7202072.1"
                     /translation="MLHFENDYNKGAHPELLNALIETNDQGLSGYGTDSYCQQAADKI
                     REVCSCPQAEVEFLVGGTQTNQVVISSMLASYEGVIAAETGHVSSHEAGAIEFSGHKV
                     LTLPSHNGKLLASEVATYIETFYADGNYQHMVFPGMVYISHPTEYGTLYSKAELEELS
                     KICKHYQIPLFIDGARLGYGLAAKDTDVDFPTIAALSDVFYIGGTKMGALAGEAVVFT
                     KKNRPKQFTTIVKQHGALLAKGRLLGLAFDRFFTDDLYLKIGKHAIDLAEELKIILEE
                     KGYSFYLKSPTNQQFVIVENTKLADLAKNVAYSFWEKYDDHHTVIRLATSWSTSREDV
                     TALRNVL"
     gene            complement(1172..2182)
                     /gene="tsaD"
                     /locus_tag="VBE22_000599"
     CDS             complement(1172..2182)
                     /gene="tsaD"
                     /locus_tag="VBE22_000599"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEO7202073.1"
                     /translation="MKDRYILAVESSCDETSVAILKNDKELLANIIASQVESHKRFGG
                     VVPEVASRHHVEVVTTCFEDALQEAGIVASDLDAVAVTYGPGLVGALLVGMAAAKAFA
                     WANKLPLIPVNHMAGHLMAARDVKELQYPLLALLVSGGHTELVYVSGPGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMIKEDHLEFSFSGLKSA
                     FINLHHNAEQKGEALVLEDLCASFQAAVLDILLAKTQKALLKYPVKTLVVAGGVAANQ
                     GLRERLATDISPDIDVVIPPLRLCGDNAGMIALAAAIEFEKENFASLKLNAKPSLAFE
                     GL"
     gene            complement(2258..2665)
                     /gene="rimI"
                     /locus_tag="VBE22_000600"
     CDS             complement(2258..2665)
                     /gene="rimI"
                     /locus_tag="VBE22_000600"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEO7202074.1"
                     /translation="MESSEQALAIWSVLSDVYDKSPWSLSQISEDLKKDSTDYFFVYN
                     DGEVIGFLALQQLVGEVEITNIAVKKNYQGKGYAYQLMSMIADIEVPVFLEVRYSNIV
                     AQKLYERCGFVVLGKRKNYYHDPIEGAIVMRKG"
     gene            complement(2667..3359)
                     /gene="tsaB"
                     /locus_tag="VBE22_000601"
     CDS             complement(2667..3359)
                     /gene="tsaB"
                     /locus_tag="VBE22_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEO7202075.1"
                     /translation="MMKVLAFDTSSKALSVAVLNNMECLATVTINIKKNHSINLMPAI
                     DFLMQSIDLEPQDLDRIVVAEGPGSYTGLRVAVATAKMLAYTLKIDLVGVSSLYALTN
                     GFSENDLLVPLIDARRNNVYVGFYQNGDTVKPDCHTSLEEVLQEVGNKANVHFVGEVA
                     AFFDQIKKALPHAKITETLPCAVAIGRKGQKMESVNVDAFVPRYLKRVEAEENWLKNH
                     CETNTEEYIKRV"
     gene            3538..3768
                     /locus_tag="VBE22_000602"
     CDS             3538..3768
                     /locus_tag="VBE22_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEO7202076.1"
                     /translation="MIYKVFYQETKERNPRREQTKTLYVTIDAANELEGRIAARKLVE
                     ENTAYNIEFIELLSDKHLEYEKETGVFELTEF"
     gene            3822..5501
                     /locus_tag="VBE22_000603"
     CDS             3822..5501
                     /locus_tag="VBE22_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO7202077.1"
                     /translation="MSNINLKPEEVGVYAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENIDRIKALVITHGHEDHIGGIPFLLKQANLPIYAGPLA
                     LALIKGKLEEHGLLRDATLYEIHANTELTFKNLSVTFFRTTHSIPEPLGIVIHTPQGK
                     VICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKIVGQSIM
                     KIIEGIDGRIIFASFASNIFRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKVHAGLAVDTGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRHDLSEDGVVLAVATVDFDSKMILAGPDILSRGFIYMRESGDLIRESQH
                     ILFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDK"
     gene            complement(5663..6172)
                     /locus_tag="VBE22_000604"
     CDS             complement(5663..6172)
                     /locus_tag="VBE22_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7202078.1"
                     /translation="MEENMNIKQLKSKTMLGTVALVSAFSFASTNADANTYNYAVDVD
                     YLASAEEIAQAHPASNTFPLGQCTWGVKEMATWAGNWWGNGGDWAASATSAGYTVGTQ
                     PRVGSIVCWTDGSYGHVAYVTAVDPVTNKIQVLESNYAGHQWIDNYRGWFDPQNTATP
                     GVVSYIYPN"
     gene            complement(6320..7666)
                     /gene="glnA"
                     /locus_tag="VBE22_000605"
     CDS             complement(6320..7666)
                     /gene="glnA"
                     /locus_tag="VBE22_000605"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEO7202079.1"
                     /translation="MTITAEDIRREVKEKNVTFLRLMFTDILGVMKNVEIPATDEQLD
                     KVLSNKAMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     EPFAGDPRGNLKRNMKRMQEMGYKSFNLGPEPEFFLFKMDENGNPTLDVNDKGGYFDL
                     APTDLADNTRREIVNVLTQMGFEVEASHHEVAVGQHEIDFKYDDVLKACDNIQLFKLV
                     VKTIARKHGLYATFMAKPKFGINGSGMHCNMSLFDNEGNNAFFDPEDPRGMQLSEDAY
                     YFLGGLMEHAYNYTAIINPTVNSYKRLVPGYEAPVYIAWAGRNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALSVLLGSGLEGIENKIEAPEPIETNIYAMTVEERRQAGIVDL
                     PSTLHNALKALEEDEVVKAALGTHIYTNFLDAKRIEWASYATYVSQWEIDNYLDLY"
     gene            complement(7700..8071)
                     /locus_tag="VBE22_000606"
     CDS             complement(7700..8071)
                     /locus_tag="VBE22_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7202080.1"
                     /translation="MKERELRRTMAVFPIGTVMKLTDLTARQIRYYEDQGLITPERTE
                     GNRRMFSLNDMDRLLEIKDFISDGLHISDIKNEYMQRQHKSKEKQKSLSDAEVRRLLQ
                     DELRNQGRFSSPSQHIGNMHL"
     gene            complement(8151..8690)
                     /locus_tag="VBE22_000607"
     CDS             complement(8151..8690)
                     /locus_tag="VBE22_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEO7202081.1"
                     /translation="MKTLKKLLSNYKFDIKKFKLGMRTFKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMINVACNNKSGIIGGTAALLIITLSIPSGETILYVFARIFETFCGVFIA
                     MMVNTDIEILRKKLKNNKL"
     gene            complement(8953..10149)
                     /locus_tag="VBE22_000608"
     CDS             complement(8953..10149)
                     /locus_tag="VBE22_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEO7202082.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGAKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQLGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(10284..11153)
                     /locus_tag="VBE22_000609"
     CDS             complement(10284..11153)
                     /locus_tag="VBE22_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEO7202083.1"
                     /translation="MKTTKVFSVLSLSVAAFMMTACQSNHSTKKADMSPDKVEQKTKF
                     KNISLSSNKLLAKENTMSVLWYQNSAEAKALYLQGYNVAKMKLDDWLQKPSEKPYSII
                     LDLDETVLDNSPYQAKNIKDGSSFTPESWDKWVQKKSAKAVAGAKAFLKYANEKGIKI
                     YYVSDRTDAQVDATKENLEKEGIPVQGKDHLLFLKKGMKSKESRRQAVQKDTNLIMLF
                     GDNLVDFADFSKSSSTDREQLLTKLQSEFGSKFIVFPNPMYGSWESAIYQGKHLDVQK
                     QLKERQKMLHSYD"
     gene            complement(11362..12372)
                     /gene="gap"
                     /locus_tag="VBE22_000610"
     CDS             complement(11362..12372)
                     /gene="gap"
                     /locus_tag="VBE22_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEO7202084.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGQFVKVSAEREPANIDWATDGVEIVLEATGFFAS
                     KEKAEQHIHENGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALQDNFGVKQGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLEKDVTVEEVNAAMKAAANDSYGYTED
                     PIVSSDIVGISYGSLFDATQTKVQTVDGNQLVKVVSWYDNEMSYTSQLVRTLEYFAKI
                     AK"
     gene            complement(12576..14654)
                     /gene="fusA"
                     /locus_tag="VBE22_000611"
     CDS             complement(12576..14654)
                     /gene="fusA"
                     /locus_tag="VBE22_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEO7202085.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQSLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYVDQANEYREKLVEAV
                     ADTDEDLMMKYLEGEEITNEELMAAIRRATINVEFYPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDEEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     SKVILESIEVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQVVRAFVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNSGE"
     gene            complement(14809..15279)
                     /gene="rpsG"
                     /locus_tag="VBE22_000612"
     CDS             complement(14809..15279)
                     /gene="rpsG"
                     /locus_tag="VBE22_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEO7202086.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFEAIKEATGTDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(15301..15714)
                     /gene="rpsL"
                     /locus_tag="VBE22_000613"
     CDS             complement(15301..15714)
                     /gene="rpsL"
                     /locus_tag="VBE22_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEO7202087.1"
                     /translation="MPTINQLVRKPRKSKVEKSDSPALNIGYNSHRKVHTKLSAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(15933..16745)
                     /gene="purR"
                     /locus_tag="VBE22_000614"
     CDS             complement(15933..16745)
                     /gene="purR"
                     /locus_tag="VBE22_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEO7202088.1"
                     /translation="MKLRRSERMVVISNYLINNPYTLTSLNTFASKYGAAKSSISEDI
                     AIIKKAFEQAQIGDIKTVTGASGGVIFTPTIAEAEAKEIVEELRQRLSENDRILPGGY
                     IYLSDLLSTPKMLQSIGRIVANAYRGQKIDAVMTVATKGVPLANAVANVLDVPFVIVR
                     RDLKITEGSTVSVNYASGSSGRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTVSGMISL
                     LSEFDSTLVGVAVFAENAQEQREKMAYKSLLRVSEIDVKNNRVSVEAGNIFD"
     gene            complement(16842..17783)
                     /locus_tag="VBE22_000615"
     CDS             complement(16842..17783)
                     /locus_tag="VBE22_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEO7202089.1"
                     /translation="MKINQMKKDELFEGFYLIKKAEVRKTRAGKDFIAFTFQDDTGEI
                     SGNMWDAQTYNVEEFVAGKIVHMKGRREVYNGTPQVNQITLRNIKDGEPNDPRDFKEK
                     PPINVDNVREYMEQMLFKIENATWQRVVRALYRKYNKEFFTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPELNKSLMFAGIMLHDLAKVIELSGPDNTEYTIRGNLIGHIS
                     LIDEELTKILAELNIDDTKEEVTVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALNRVNEGEMTNRIFAMDNRSFYKLNIK"
     gene            complement(17773..19047)
                     /locus_tag="VBE22_000616"
     CDS             complement(17773..19047)
                     /locus_tag="VBE22_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEO7202090.1"
                     /translation="MLVIILIIVLASLTVTIISYQKMTELTKSVEKQLEDNADNLSDQ
                     LTYQIEAAQKDQILTLTNQLNRMQQEIYQLLTDMRTELNQHLTESRDRSDKRLELINS
                     NLSQSVQKMQDSNEKRLDQMRQTVEEKLEKTLQTRLQTSFETVSRQLESVNQGLGEMK
                     TVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTVSQYEREFPTVSGSSERVEYAI
                     KLPGNGQGDYIYLPIDSKFPLEDYYRLEDAYELGDKVQIELYRKSLLASIRKFAKDIN
                     NKYLNPPETTNFGIMFLPTEGLYSEVVRNATFFDSLRRDENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNANDISKILGNVKVEFGKFGGMLSKAQKQLNTASKSIDNLLTTRTNAI
                     IRALNTVEEHQDQATTSLLNLPITEEEEINEN"
     gene            complement(19049..19681)
                     /locus_tag="VBE22_000617"
     CDS             complement(19049..19681)
                     /locus_tag="VBE22_000617"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEO7202091.1"
                     /translation="MTKIALFAGGDLTYFEYDFDYFVGIDRGSLFLLKNGLSLDMAVG
                     DFDSITEDELLYIKHYCSNIVSASAEKNDTDTELALKTIFKEFPEAQVTVFGAFGGRI
                     DHMMSNIFLPSDTDLEPFMSQIRLKDEQNIVTYLPSGKNQVSRIEGMSYVSFMPESES
                     TLQISGAKYELNKSNYFKKKMYSSNEFMTSPIEVELKDGYLIIIYSKDRG"
     gene            complement(19674..20336)
                     /locus_tag="VBE22_000618"
     CDS             complement(19674..20336)
                     /locus_tag="VBE22_000618"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO7202092.1"
                     /translation="MSTNKIAPSILAADYANFANELKRIEETTAEYVHIDIMDGQFVP
                     NISFGADVVSSMRKHSKLVFDCHLMVVDPERYIEDFAQAGADIMTIHVEATKHIHGAL
                     QKIKEAGMKAGVVINPGTPVESLIPILDLVDQILIMTVNPGFGGQAFIPEMMSKVKTV
                     AAWRKEYGHHYDIEVDGGIDNTTIKAAAEAGANVFVAGSYLFKASDLPAQVETLRVAL
                     DD"
     gene            complement(20343..21215)
                     /gene="rsgA"
                     /locus_tag="VBE22_000619"
     CDS             complement(20343..21215)
                     /gene="rsgA"
                     /locus_tag="VBE22_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEO7202093.1"
                     /translation="MQGRIVKSLAGFYYVESDGVVYQTRARGNFRKKGQIPYVGDWVE
                     FSSQDQSEGYILSIEERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEYK
                     MIQPIIYISKLDLLDDLVVIDDIREHYQNIGYVFCYSQEELLPLLADKVTVFMGQTGV
                     GKSTLLNKIAPELKLETGEISDSLGRGRHTTRAVSFYNVHKGKIADTPGFSSLDYEVD
                     NAEDLNESFPELRRLSHFCKFRSCTHTHEPKCAVKEALTQGQLWQVRYDNYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            complement(21371..22222)
                     /locus_tag="VBE22_000620"
     CDS             complement(21371..22222)
                     /locus_tag="VBE22_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO7202094.1"
                     /translation="MLVDKKWRFEDSASYFACPKCQNPLIKESNSLKCSDNHCFDLSK
                     FGYVNLLGGKKIDEHYDKKSFENRQLVLENGYYNHILEAISKVLENNSQFHSVLDIGC
                     GEGFYSRQLVNKYEKTFLAFDISKDSIQLAAKSDQSRLVKWFVSDLANLPIQDSSIDI
                     ILDIFSPANYKEFRRVLSDDGILVKVVPVAEHVQELREKASQYLKQKDYSNQKILDYF
                     RENFEIISEQKVVQSYNCSQQERQAFIDMTPLLFSVDKTTIDWASISEITVGALIVIG
                     KKRSVSK"
     gene            complement(22226..23098)
                     /gene="rsmA"
                     /locus_tag="VBE22_000621"
     CDS             complement(22226..23098)
                     /gene="rsmA"
                     /locus_tag="VBE22_000621"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEO7202095.1"
                     /translation="MRIADKTVTRAILERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DKGVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLARFDNVQVVNQDIL
                     KADLQTQIQAFKNPDLPIKVVANLPYYITTPILMHLIEGKIPFAEFVVMMQREVADRI
                     SAMPNTKAYGSLSIAVQYYMTAKVSFIVPRTVFVPAPNVDSAILKMVRRDQPVVSVQD
                     EDFFFRVSKVAFVHRRKTLWNNLTSHFGKSEDTKAKLEKALEIAKIKPSIRGEALSIP
                     DFASLADALKEVGI"
     gene            complement(23125..23574)
                     /locus_tag="VBE22_000622"
     CDS             complement(23125..23574)
                     /locus_tag="VBE22_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO7202096.1"
                     /translation="MCLICQRIEMIERNENPYFVKEYETGYLVLGDHQYFQDYCLFLS
                     KKHVTELHELPRDWRNQYLSEMADASEIVAKAFRADKMNIESLGNGDAHLHFHLFPRK
                     TGDLRNYGHNGKGPVWWYPFEKMYADSVRATGAEIEKLKEKLLDVLG"
     gene            complement(23578..24144)
                     /gene="rnmV"
                     /locus_tag="VBE22_000623"
     CDS             complement(23578..24144)
                     /gene="rnmV"
                     /locus_tag="VBE22_000623"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEO7202097.1"
                     /translation="MKKIDIQEVIVVEGKDDTANLRRFYNVDTYETRGSAIDEDDLER
                     IERLHNLRGVIIFTDPDYNGERIRKIIMKAIPTVRHAFLNRDEAKPVSKTKGRSLGVE
                     HASFEDLQKALSKVTQHFDDEDHFDITQADLIRWGFITASDSRKRREYLGSQLRIGYS
                     NGKQLLKRLRLFGVTKAEVEECMEKYEG"
     gene            complement(24131..24913)
                     /locus_tag="VBE22_000624"
     CDS             complement(24131..24913)
                     /locus_tag="VBE22_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEO7202098.1"
                     /translation="MIKIFDTHTHLNVENFEGKIDEEINLASELGVTKMNVVGFDQDT
                     ISKSLELSSQYAQVYSTIGWHPTEAGSYDDNIESMIISHLENPKVIALGEIGLDYYWM
                     EDPKDIQIEVFKRQIELSKEYNLPFVVHTRDALEDTYEVIKESGVGPFGGIMHSFSGS
                     LEMAQKFIDLGMMISFSGVVTFKKALDVQEAARELPLDKILVETDAPYLAPVPKRGRE
                     NKTAYTRYVVEKIAELRGITVEEVAEATYQNAVRIFRLDEKN"
     gene            complement(25095..25376)
                     /locus_tag="VBE22_000625"
     CDS             complement(25095..25376)
                     /locus_tag="VBE22_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202099.1"
                     /translation="MSLMWDVLTGKESVRTLKKQLDKELIHSVYAPRKRSKFHDIITS
                     HHKEIDEVAINTKTAIHKVANQLDEAIKGEFRSKVEESIESNQQKYNHL"
     gene            complement(25386..25778)
                     /locus_tag="VBE22_000626"
     CDS             complement(25386..25778)
                     /locus_tag="VBE22_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202100.1"
                     /translation="MALIDIIEKQLADTQRKISDLDDAYHHSCCQFEEKLDDLSVRKN
                     KITNMLQETYDAVEYDLRYSNDSSDMMTLNRILDSYHDDLEQAYHKEYYALSAQEEEY
                     RANYIRQRSEHELTFEELQREKKRELMK"
     gene            complement(25769..27061)
                     /locus_tag="VBE22_000627"
     CDS             complement(25769..27061)
                     /locus_tag="VBE22_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7202101.1"
                     /translation="MEKVLTDLDRVNIAKQEYELGSQLDTLVKIMSQDKVLPIGKVAH
                     VQDGGKETGEQIYTITPNGTLDKPEDVKEVTVLFKGSTAPFGGDDWKTDWFKNDIPIA
                     SKLLLKKFGSQSVSHKQGTKQLEQSAHLLKEVMNKYPNAKISVYGHSLGSMNAQYAIA
                     ELDDNQIYRVQGAWIYNGPNLYSILTDKQKEQVDKIKGVVNNYVDSKDKIGIGYPKEG
                     SDNAVGIVKIVDSKAVSGGSWVANFTNQHIWGGYQYNQDGSLKLAEDKFAYKENYSVV
                     HTNVNQRMHAIKAKLSSFKSTGKEVSKNQAIFLDSQQAKVISSGLVTISKEAVEEVKK
                     VEQKAVAKAEELYQSLKVAPTGVTELSPQELEEAYAQSGVSYASIVGEAQTFFSNKIQ
                     AAESISHAYVELENQINKGVEAMLQQDSNLSGEFKQWH"
     gene            complement(27061..27453)
                     /locus_tag="VBE22_000628"
     CDS             complement(27061..27453)
                     /locus_tag="VBE22_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 domain-containing protein"
                     /protein_id="HEO7202102.1"
                     /translation="MKSIKLLSFIVIILLGLGFTVGGLKVTENNKQQELWEIANSKDA
                     KNAYQKWIYAEDKDAFKENAVIKSYDIDKESIKKNPMGGISVRLIINKDPNLYITCNL
                     DKDNQGRLVSQSSHQSPQLTHLLESRGH"
     gene            complement(27628..28890)
                     /gene="dltD"
                     /locus_tag="VBE22_000629"
     CDS             complement(27628..28890)
                     /gene="dltD"
                     /locus_tag="VBE22_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEO7202103.1"
                     /translation="MLKRLGKVFGPLVCALLLLVGLYLVFPVSQPHHLGKEKNSAVAL
                     TKAGFKSRVQKVRAFSDPKANFVPFFGSSEWLRFDAMHPSVLAEAYKRPYIPYLLGQK
                     GAASLTQYYGIQQIKGQIKNKKAIYVISPQWFVRKGANKGAFQNYFSNDQTIRFLQNQ
                     TGTTYDRYAARRLLKLYPEASMSDLIEKVADGQKLSNKDKQRLKFNDWVFEKTDAIFS
                     YLPLGKTYNQVIMPHVGKLPKAFSYNHLSRIASQDAKVATRSNQFGIDDRFYQTRIKK
                     HLKKLKGSQRHFNYTKSPEFNDLQLVLNEFSKQNTDVLFVIPPVNKKWTDYTGLDQKM
                     YQKSVEKIKHQLQSQGFNHISDLSRDGGKPYFMQDTIHLGWNGWLELDKHINPFLTEE
                     NSKPNYHINNKFLKRSWAKYTGRPSDYK"
     gene            complement(28883..29122)
                     /gene="dltC"
                     /locus_tag="VBE22_000630"
     CDS             complement(28883..29122)
                     /gene="dltC"
                     /locus_tag="VBE22_000630"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEO7202104.1"
                     /translation="MDIKSEVLAIIDDLFMEDVSSMMDEDLFDAGVLDSMGTVELIVE
                     LESHFNIDIPIAEFGRNDWNTANKIVAGVTELCNA"
     gene            complement(29137..30402)
                     /gene="dltB"
                     /locus_tag="VBE22_000631"
     CDS             complement(29137..30402)
                     /gene="dltB"
                     /locus_tag="VBE22_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEO7202105.1"
                     /translation="MIQAFLEKLPHLDVYGNPQYFFYLILAVLPIYIGLFFKKRFALY
                     EIIFSLSFIVMMLTGSTFNQLKSLLAYVVGQSLLVFIYKAYRKRFNHTLVFYVTVCLS
                     IFPLFLVKLIPAISEDGHQSLFGFLGISYLTFRAVAMIIEMRDGVLKEFTLWEFLRFL
                     LFFPTFSSGPIDRFKRFNEDYINIPDRNELLDMLGQAIHYLMLGFLYKFILAYIFGSL
                     IMPPLKELALEQGGVFNWPTLGVMYAFGFDLFFDFAGYTMFALAISNLMGIKSPINFD
                     KPFKSRDLKEFWNRWHMSLSFWFRDFVFMRLVKLLVKNKVFKNRNVTSSVAYIINMLL
                     MGFWHGLTWYYIAYGLFHGIGLVINDAWVRKKKNINKERRLAKKPLLPENKWTYALGV
                     FITFNVVMFSFLIFSGFLDLLWFPQPHNK"
     gene            complement(30399..31934)
                     /gene="dltA"
                     /locus_tag="VBE22_000632"
     CDS             complement(30399..31934)
                     /gene="dltA"
                     /locus_tag="VBE22_000632"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEO7202106.1"
                     /translation="MIHDMIKTIEHFAETQADFPVYDILGEVHTYGQLKVDSDSLAAH
                     IDSLGLVEKSPVLVFGGQEYEMLATFVALTKSGHAYIPVDQHSALDRIQAIMTVAQPS
                     LIISIGEFPLEVDNVPILDVSQVSAIFEEKTPYEVTHSVKGDDNYYIIFTSGTTGLPK
                     GVQISHDNLLSFTNWMISDDEFSVPERPQMLAQPPYSFDLSVMYWAPTLAMGGTLFAL
                     PKTVVNDFKKLFATINELPIQVWTSTPSFADMALLSNDFNSETLPQLTHFYFDGEELT
                     VKTAQKLRQRFPKARIVNAYGPTEATVALSAVAITDEMLETCKRLPIGYTKDDSPTYV
                     IDEEGHKLPNGEQGEIIIAGPAVSKGYLNNPEKTAEAFFQFEGLPAYHTGDLGSMTDE
                     GLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSQYVKSAVAVPRYNKDHKVQNLLAYIV
                     LKEGVRDDFERDLDLTKAIKEDLKDIMMDYMMPSKFIYREDLPLTPNGKIDIKGLMSE
                     VNK"
     gene            complement(31947..32069)
                     /locus_tag="VBE22_000633"
     CDS             complement(31947..32069)
                     /locus_tag="VBE22_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEO7202107.1"
                     /translation="MKPYLSFIGRTLLYFGILLLLIYFFAYLGRGQGSFIYNEF"
     gene            complement(32080..33267)
                     /locus_tag="VBE22_000634"
     CDS             complement(32080..33267)
                     /locus_tag="VBE22_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7202108.1"
                     /translation="MFSDLRKKFVFLTMSILIVVVLFLFAVSNRYNQYWDEYDAYRIV
                     KLVAKNDYLGIPGDEPIALVTIDNQKMVKIQSNNTDLTNDVIEKSSLKLLEQGKKSRK
                     WKSFIYSIKEYKDKTYTIAIMDLASYEVPYARRFLILVFTIFGFCLLAAVSLYLSRFI
                     VGPVETEMTREKQFVSDASHELKTPIAAIRANVQVLEQQIPGNRYLDHVVSETKRMEF
                     LIEDLLNLSRLDEKRSKVNFKKLNLSVLCQEVLLTYESLAYEEEKCLNDTIEDDVWIV
                     GEESQIKQILIILLDNAIRHSLSKSEIQFSLKQARRKAILTISNPSAIYSKEVMDNLF
                     ERFYQAKDDHADSLSFGLGLSIAKAIVERHKGRIRAYQEKDQLRLEVQLPIDGFWTNT
                     MIN"
     gene            complement(33267..33941)
                     /locus_tag="VBE22_000635"
     CDS             complement(33267..33941)
                     /locus_tag="VBE22_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7202109.1"
                     /translation="MRLLVVEDEKSIAEAIQALLADKGYSVDLAFDGDDGLEYILTGL
                     YDLVLLDIMLPKRSGLSVLKRVREAGLETPIIFLTAKSQTYDKVNGLDLGADDYITKP
                     FEADELLARIRLRTRQSSLIRANQLRLGNIRLNTDSHELESKESSVKLSNKEFLLMEV
                     FMRNAKQIIPKNQLISKVWGPSDNSEYNQLEVFISFLRKKLRFLKADIEIITTKGFGY
                     SLEERT"
     gene            complement(34297..34431)
                     /gene="rpmH"
                     /locus_tag="VBE22_000636"
     CDS             complement(34297..34431)
                     /gene="rpmH"
                     /locus_tag="VBE22_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEO7202110.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            complement(34615..>35918)
                     /locus_tag="VBE22_000637"
     CDS             complement(34615..>35918)
                     /locus_tag="VBE22_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HEO7202111.1"
                     /translation="LFGVLLLMTPFKYNGMTTVAVSVISKTINQWINAVFPIHYIILL
                     IIFISCVLALCYRLFRPSFIEKNDLLKEISDITIFWLIIRLIGLALGLMTVLHIGPEM
                     VWGKETGGLILFDLIGGLFTIFLAAGFILPFLTEFGLLEFVGVFLTPIMRPFFQLPGR
                     SAVNCVASFVGDGTIGIALTDKQYVEGYYTSREAATISTTFSAVSITFCLVVLQNVGL
                     TDYFGHFYLTVIISSVVCASIIPYIPPLSRQSNSYYTKPKKETSEVIPDGYNKIEWGL
                     KLAIDKSKANGNMKTFVNNASKTVLSLWFGVMPTIMTVGTIALIISVSTPIFKILGTP
                     FLPFLELLGIPEADIASQTMIVGFSDMVVPSIMAAEIHSEMTRFIVATVSIVQLIYMS
                     ETGAVILGSKIPINILELFIIFIERTIISLPIIVLMAHLFF"
BASE COUNT        11171 a         7260 c         5755 g        11732 t
ORIGIN      
        1 tgaataatat ttcttttatt aaaaactcac aagacatttc ttaaagcagt tacatcttcg
       61 cgactagttg accaactagt tgctagacgt attacagtat gatggtcatc atatttttcc
      121 caaaaactat aagctacatt ctttgctaaa tctgctagtt ttgtattttc tacaataaca
      181 aattgttgat tagttggtga ttttaaatag aacgagtaac ctttttcttc taaaatgatt
      241 tttaattcct ctgctaagtc aatcgcatgc tttccaatct ttaagtaaag gtcatccgta
      301 aagaaacgat caaaagcaag ccccagcaat cttcccttgg ctaataacgc accatgctgc
      361 tttacaattg tagtaaactg ttttggacga tttttctttg tgaaaacaac tgcttcccca
      421 gcaagagccc ccattttggt tccaccaatg taaaaaacat ctgataaggc tgcaatcgtt
      481 gggaaatcaa catctgtatc tttcgcagcc aaaccatacc ccagacgagc accgtcaata
      541 aataagggga tttgataatg tttacaaatc ttcgataact cttctagttc tgctttacta
      601 taaagtgttc catattctgt tggatgtgat atataaacca ttcctggaaa aaccatgtgt
      661 tggtaattac catcagcata aaaagtctca atataagttg caacttcgct tgcgagtaat
      721 ttcccattat gagaaggtaa agtcaatacc ttatgacctg aaaattctat ggctcctgct
      781 tcatgactac tcacatgacc tgtttctgca gcaataactc cttcataaga agcaagcata
      841 ctactgataa ctacttgatt tgtttgagta ccacccacta aaaactcaac ttcagcttgt
      901 ggacaggaac agacttctcg aattttatct gccgcttgtt gacagtaact atctgtccca
      961 tatcccgata gaccttgatc attagtttca ataagggcat tcaaaagctc tggatgagct
     1021 cctttattat aatcattttc aaaatgtaac attccttaac tcctttatta gttaaaaaaa
     1081 atagcgacaa tatcatttgc taattttttg aatattcaat tattatacca aataaaaaat
     1141 atcaaaacaa gcgttttgat attatgaaca cctataaacc ttcaaaagct aagctaggtt
     1201 tggcattcaa ttttaaagaa gcaaaattct ctttttcaaa ctctatcgct gctgctaatg
     1261 caatcattcc tgcattatcc ccacataatc taagaggagg aataaccaca tcaatatcag
     1321 gagaaatatc agtagccaag cgttcccgaa gtccttgatt agctgcaact ccaccagcaa
     1381 cgactaaagt tttcactgga tactttaaca aagctttttg agttttggcc aataaaatat
     1441 ccaaaacagc cgcctgaaag gaagcacata aatcttcaag aaccaatgct tcaccctttt
     1501 gttctgcatt atgatgtaaa ttgataaatg cagattttaa tccagaaaaa gaaaattcaa
     1561 gatgatcttc tttgatcatc gctctaggaa aatggtaagt atcttgaccc ttatgagcta
     1621 actgatcaat ctcgcgacct gctggataag ttaagcccat aacacggcct actttatcat
     1681 aagcttctcc aacagcatca tcccgagttt ctcctactat tttgtaatct cccggtccag
     1741 aaacatatac taattctgta tgtcccccac tgacaagcaa agctaacaat gggtattgaa
     1801 gttccttaac gtcacgtgct gccattaaat gacctgccat gtggtttaca ggaattagag
     1861 gtaatttatt tgcccaagcg aaagcttttg ctgcagccat acctaccaat aaggctccta
     1921 ctaatcccgg accatatgtt acagcaacag catccaaatc gctagcaaca atacctgctt
     1981 cttgaagagc atcctcaaaa caggttgtta ctacttcaac gtgatgacgg cttgccactt
     2041 caggaacaac accaccaaaa cgtttgtgac tttcaacttg acttgcaata atattagcta
     2101 gtaactcttt atcatttttt aaaatagcaa cactagtttc atcacatgaa ctttccactg
     2161 ctaaaatata tctatctttc ataatgatat taatccatca atatcgtctt ttccattagt
     2221 attgaggact attcatatcc tttctatcat caactattta tcccttgcgc atcactatag
     2281 caccttcaat tggatcatgg taataatttt tgcgctttcc taaaactaca aaaccacacc
     2341 tctcataaag tttttgtgct acgatatttg aataacgaac ctcaagaaaa actggcacct
     2401 ctatatcagc aatcattgac attaactggt aagcataacc tttaccttga taattttttt
     2461 taacagcaat attagtaatt tctacttcac ctaccaattg ttgaagagcc aaaaaaccaa
     2521 tgacctcacc gtcattataa acaaaaaaat aatctgttga atcttttttt aaatcttctg
     2581 aaatctgcga taaactccac ggagatttgt catagacatc agatagtacg ctccatatag
     2641 ccagtgcttg ttctgatgat tccatctcaa actctcttaa tatattcttc tgtattcgtt
     2701 tcacagtggt tttttaacca attttcctca gcttcaactc gttttaagta tcgtggaaca
     2761 aacgcatcta cattaacgct ttccattttt tgtcctttgc gcccaattgc cactgcacaa
     2821 ggtaaagttt ctgtaatttt agcatgtggt aaggctttct taatctgatc aaaaaatgct
     2881 gcaacctctc cgacaaaatg aacattggct ttattcccca cctcttgtaa gacttcttca
     2941 agagaagtgt gacagtctgg tttaacagta tcaccatttt gatagaaacc aacataaaca
     3001 ttgttacgtc gtgcatctat aagtggtacc aacaaatcat tttctgaaaa tccatttgtt
     3061 aaagcgtaca ggctagatac tccaactaag tcaatcttaa gcgtataagc tagcattttt
     3121 gctgtagcaa cagctacacg taagcccgta taagatcctg gaccctctgc cactacgata
     3181 cggtccaaat cttgaggttc taaatcaatt gattgcatta aaaaatcaat ggctggcatc
     3241 aaattaatgc tatgattctt tttgatattg atagtgacag tcgctaaaca ttccatattg
     3301 tttagtacag ccactgatag tgctttgctt gaagtatcaa aggctaaaac tttcatcatt
     3361 taacttccct tacttgattc tacctcttat tttatgatat aatcatgata attgcaacga
     3421 atcaaggtta attctattac tctataaaaa cttaataatg gtttgtcatt gtctcactta
     3481 acggcattga cccatttttt tatagttaga aaaaccgaga gataagaaag gattattatg
     3541 atttataaag ttttctatca agaaacaaaa gaacgcaacc cacgtcgtga gcaaaccaaa
     3601 acactttacg ttactattga tgcagctaat gaattagaag gtcgcattgc tgcacgtaaa
     3661 ctagttgaag aaaatacagc ttacaatatc gaattcatcg agttactttc agacaaacac
     3721 cttgaatatg aaaaagaaac tggtgttttc gagctcactg agttctagaa aggagttaaa
     3781 cataatagct agagtaatct acccattatg ataaacttat tatgtcaaat atcaacttaa
     3841 aacccgaaga agttggggtt tatgcgattg gtggtctcgg tgaaatcgga aaaaatactt
     3901 acggtatcga atatcaagac gaaattatta ttgtagacgc tggtattaaa ttccctgaag
     3961 atgatctttt gggaatagat tatgttatcc cagactattc atatatcgtt gaaaacattg
     4021 accgtatcaa ggcccttgtt ataactcatg ggcacgagga tcacatcggt ggtattcctt
     4081 ttctattaaa acaagctaat ttaccgattt atgcaggtcc tctcgctctt gctctaatca
     4141 aaggtaagtt agaagaacat ggtttacttc gtgacgctac attatacgaa atccacgcta
     4201 atacagagct gacttttaaa aacttaagcg ttacattttt ccgcactact cactccattc
     4261 ctgaaccatt aggtattgtt attcataccc ctcaaggtaa ggttatttgt acaggtgact
     4321 tcaaatttga ctttacacct gttggggagc ccgctgactt acatcgtatg gctgctctag
     4381 gtgaagatgg tgtcctctgc cttttatcag actcgacaaa tgctgaagtt ccaaccttca
     4441 ctaattccga aaaaattgtt gggcaatcta ttatgaagat tatcgaaggt atcgacggac
     4501 gtattatttt cgcctctttt gcttcaaaca tcttccgact ccaacaggct gctgaagctg
     4561 ctgttaaaac tggtcgtaaa attgctgttt ttggacggtc aatggaaaag gcaattgtta
     4621 acggtattga acttggctat attaaagttc caaaaggtac cttcattgag ccaagcgaac
     4681 ttaaaaatct tcatgctagt gaagttttaa ttatgtgtac aggtagtcaa ggtgagtcta
     4741 tggcagcact tgctcgtatc gctaacggta ctcatcgtca agttactttg caacctggtg
     4801 atactgtgat tttctcatca agcccaatcc ctggtaatac aacaagtgtc aataaattaa
     4861 tcaataccat tcaagaagct ggtgtcgatg ttattcatgg taaaattaac aatatccata
     4921 cctccggcca tggtggtcaa caagaacaaa aattaatgtt acgcttgatt aaaccaaagt
     4981 atttcatgcc cgtacacggt gagtatcgca tgcaaaaagt ccatgctggt ttagcagttg
     5041 acacaggtat tcctaaagag aatatcttca ttatggaaaa cggtgatgtt ttagctttaa
     5101 cgagcgactc tgctcgcatt gctggacatt tcaatgctca agatatctac gttgatggga
     5161 atggtattgg cgatattggt gcggcagttc tacgtgatcg ccacgactta tctgaagatg
     5221 gtgttgtcct tgccgttgct accgttgatt tcgattcaaa aatgatttta gctggaccag
     5281 acatccttag ccgaggcttt atctatatga gagaatctgg ggaccttatt cgcgaaagcc
     5341 aacatatctt attcaatgct attcgtattg cactgaaaaa taaagatgca agtatccaat
     5401 cagtaaatgg agctattgtt aatgccttac gaccattctt atatgaaaaa actgagcgtg
     5461 agcctatcat tattcctatg gtccttaccc ctgataaata aacaaaagaa cggaaaatac
     5521 cttttccgtt ctttttaatt gcctatatta gcaaaataag ttaatatagc aaattacttc
     5581 tgggataact atagaatata aaagaactcc atccaaacag tatcgtactt ttataccgtc
     5641 tatgtgagga gttctttata gattaattcg gataaatgta gctaactacc cctggtgtcg
     5701 ctgtattttg tggatcaaac caaccacgat aattatcaat ccattgatga cctgcataat
     5761 tagattctaa aacctgaatt ttattagtga caggatctac agctgtaaca taagccacat
     5821 gtccataact accatcagtc caacatacga tagaacccac gcgaggttgt gttcctactg
     5881 tataaccagc tgatgtggcg ctcgcagccc aatcaccacc gtttccccac cagttacctg
     5941 cccatgttgc catctccttt acaccccatg tgcattgacc tagtggaaac gtattactag
     6001 ctggatgtgc ttgagcaatt tcctccgcac ttgctaggta gtctacatct actgcatagt
     6061 tatatgtatt cgcatctgca tttgttgaag caaatgaaaa agcggacacc aaagccactg
     6121 ttccaagcat tgttttagac tttaattgtt ttatgttcat gttttcctcc atctgatgac
     6181 tcctagttta tcaaatccat ataacagaca aatgaaaacc tgtaacaagt tttaggcaat
     6241 tttttacaat aatttacaaa aaaactagtt ttagacttta caaaataaag cctaaaacta
     6301 gttataaaca ttcttatgat taatataaat ctaggtaatt atcaatttcc cattgtgaaa
     6361 cataagtcgc ataacttgcc cattcaatac gcttagcatc taagaaatta gtatagatat
     6421 gagttcctag ggccgctttt accacttcat cttcttcaag tgctttaaga gcattatgaa
     6481 gagtagatgg taaatcaaca atacctgctt gacgacgttc ttctacagtc atagcataga
     6541 tatttgtctc aattggttct ggtgcttcaa ttttattctc gattccctca agtcctgatc
     6601 ccaaaagtac tgataaagca agataaggat ttgctgttgg gtcaaccgag cgcaattcta
     6661 ggcgtgttcc catacctcga gatgcaggta cacgaataag tggcgaacga ttgcgacctg
     6721 cccaagcaat gtaaactggt gcttcgtatc ctggtaccaa acgtttataa gaattaacag
     6781 ttggattaat gatagctgtg taattgtaag cgtgttccat taacccacct aaaaagtagt
     6841 aggcatcctc tgataattgc attccacgtg gatcttctgg atcaaagaaa gcattgttcc
     6901 cttcattatc aaaaagagac atattacaat gcatacctga accattgata ccaaacttag
     6961 gttttgccat aaaagtagca tataaaccat gcttacgagc aattgtttta acaaccagtt
     7021 taaataattg aatgttgtca caggctttta aaacatcatc atatttaaag tcaatctcat
     7081 gctgtccaac tgctacttca tgatgactag cttcaacttc aaagcccatt tgtgttaaga
     7141 cattaacaat ttcacgacgt gtattatctg ctaagtctgt tggagctaag tcaaagtagc
     7201 ctcctttgtc attaacatca agtgttgggt taccattttc atccatttta aataggaaaa
     7261 attcaggttc tggtcctaag ttaaatgact tatagcccat ctcttgcata cgtttcatat
     7321 tacgcttaag atttccacgt ggatctccgg caaaaggttc tccttctgct gtatagatat
     7381 cacagattag acctgcaaca gctccatttt catctcccca aggaaaaaca atccaagtat
     7441 ctaaatcagg atataaatac atatcagatt cattgatacg aacaaagccc tcaatagaag
     7501 atccatcaaa cattgcttta tttgataaaa ctttgtctaa ttgttcatct gtagccggaa
     7561 tttcgacatt tttcattact cctaagatat ctgtgaacat caaacggagg aaggtaacat
     7621 ttttttcctt aacttcgcga cgaatatctt ctgctgtgat agtcatatag tctccttaat
     7681 atctagcttg actgcatgtt tacaaatgca tatttccaat gtgctgtgaa ggacttgaaa
     7741 aacgcccttg attacggagc tcatcttgta aaagtcgacg aacctcggca tctgataatg
     7801 acttttgttt ctctttggac ttatgttgtc tttgcatgta ttcattttta atatcagaaa
     7861 tatgcaaacc atcgctaatg aagtctttta tttccagtaa gcggtccata tcattcagtg
     7921 aaaacatgcg gcggttacct tcagtacgtt caggtgtgat taacccttga tcctcataat
     7981 agcgaatttg tctggcagtc aaatctgtca acttcataac agttccaatt gggaatacag
     8041 ccattgtacg cctaagttct ctttctttca tgatggcctc ctttcattaa accattataa
     8101 actttaaaaa gtttgatgtc aactctcatg tgagatttta tgacatcaaa ctataatttg
     8161 ttatttttta gttttttccg aagtatttca atatctgtat taaccatcat tgcaataaaa
     8221 acaccacaaa aagtttcaaa aatacgtgca aaaacatata gaattgtctc tccgcttggg
     8281 attgataaag ttataataag taatgcggct gtccccccaa taatacctga tttattgtta
     8341 caagcaacat taatcatgat acataacatg gtacaaatag gaacaattaa taatgtaacc
     8401 caaaaagctt gatgaaatat ctcattgaat gcaaaaaata ctaaggagag taagccacct
     8461 atactattac caataattct agaaaaacca aagtgcacgc ttttatcaaa gtcttctctt
     8521 aaactaaata cagcagttag agcaccaatt tgaagccctt tccaaccaaa taaatgaaaa
     8581 accaataaaa ctaaaaagac cgataagcct gttttaaatg ttctcattcc caatttaaac
     8641 tttttaatat caaatttgta attagataat aattttttta acgttttcat acctatccct
     8701 tcactatata ttctaacata tcaatccata aaaccctttt tgtaaacgtt ttatttatta
     8761 ctagtatatc ttttataaac caatttatat gaaatcgtca taaacttttg aaaattgcag
     8821 tgtcaaccaa ataccactat atctaaaaac tagtaaatga taagtgaagt caaggaaaat
     8881 tcaataactc tacattatca actaaaaaaa gacatgcatc agcatgtcct ttaatataaa
     8941 ttataatgac tattattttt cagtcaatgc tgccaaacct ggtaatactt taccttcgag
     9001 caattccatg cttgctccac caccagtaga gatccatgag aatttgtcag cacggccaag
     9061 gttgatagca gctgctgctg aatcaccacc accgatgatt gatttaacgc ctagttgttt
     9121 aacgatagcg tccattacac cgattgtacc agcttggaag tcagggtttt caaagacacc
     9181 cataggtccg ttccatacaa ctgttttagc accagtaagt gcttcatcaa atttagcgat
     9241 tgatttagga ccgatgtcaa gaccaaggaa cccttctgaa actgcttcac cttcagtgtc
     9301 gcgaacttca gtataaccag caaatgcgtt tgcttctttt gagtcaactg gcaagatcaa
     9361 tttaccgttt gatttttcaa ggaggtcttt agcaacatcc aatttgtctt cttctacaag
     9421 tgagttaccg atttcgatac cttgagcttt gtagaatgtg taagtcatac caccaccgat
     9481 aagaacttta tcagcttttt caagaaggtt ttcgataaca ccaatcttat cagaaacttt
     9541 tgagccacca agaatagcta cgaatgggcg ttctggagtt tcaactgctt cttggatgta
     9601 agcaatttcg ttttcaagaa ggaaaccagc tacagctttt tcaacgtttg ctgaaatacc
     9661 tacgtttgat gcatgagcac ggtgtgctgt accaaatgca tcgttaacga agattccatc
     9721 tccaagtgaa gcccagtatt taccaagttc ttcgtcattc ttagattctt tcttaccgtc
     9781 aacatcttca aaacgagtgt tttcaaccaa aagaacttgt ccatcttcca aagcattgat
     9841 tgcttcttct aattttgcac cacgagtaac acctgggaat acaacatctt gaccaagttt
     9901 agcagctaaa tcagcagcta ccggtgcaag tgattttcct tctttgtcag cttcttcttt
     9961 aacacgtcca aggtgagaga agaggatagc acgaccacct tgttcgatga tatacttgat
    10021 tgttggaaga gccgcagtga tacggttgtc gttagtgata acgccgtctt tcaaaggcac
    10081 attaaagtca acacgaacga ggactttttt acctttcaaa tcaacgtctt taacagtcaa
    10141 tttagccatt agtaaaactc cttatataat ttgatattat ttattataac atatttttgt
    10201 gaacagttgt aattattgat tactttttca caaatagatt tcatagattt tcctagttgt
    10261 tttcaagaga attcgtatac ttattaatca tacgaatgca acattttttg tcgttctttc
    10321 aattgttttt gaacatccag atgttttcct tgataaatag cactttccca agaaccgtac
    10381 ataggatttg ggaaaacaat aaatttacta ccaaactcac tttgaagttt agttagtagt
    10441 tgttctctat ctgtactaga tgatttagaa aaatcagcaa aatcaactaa attatctcca
    10501 aaaagcataa ttaaattagt atctttttga actgcctgac ggcgactctc tttagatttc
    10561 attccttttt taaggaaaag caagtggtct ttcccttgaa caggtatacc ttccttctct
    10621 aaattttctt tagtcgcatc aacttgagca tctgtacgat ctgagacata ataaattttt
    10681 attccctttt cattagcata cttcaaaaat gctttggcac ccgcaacagc cttagctgac
    10741 ttcttttgca cccatttatc ccaactctct ggcgtgaaac tagagccatc tttaatattc
    10801 tttgcttgat atgggctatt atctaaaact gtttcatcta aatctaagat aattgaatat
    10861 ggtttttcac tgggcttttg taaccaatca tctaacttca ttttagcaac attataacct
    10921 tgtaaatata aagccttggc ttctgcagaa ttttgatacc aaaggacaga catagtgttt
    10981 tccttagcca acagtttatt agaacttaga gaaatatttt taaatttcgt cttttgctcc
    11041 accttatctg gagacatatc agctttttta gtgctgtgat ttgactgaca tgctgtcatc
    11101 ataaatgcag caacagataa cgataaaact gaaaaaactt ttgtagtctt cataaaactt
    11161 ctcctctata ttttttcaat taaatcatac cactgcttac tttcaaaatc aaaccttatt
    11221 ttaacttata gccaatataa ttcagttttc atttcttttc tgataatttt ctataataaa
    11281 atgaaaaaac attcgtatta ttttttattt ataaaaataa aagagggata aatccctctt
    11341 ttacatcata ttaattaaaa attattttgc aatttttgca aagtattcaa gtgtacgaac
    11401 aagttgtgaa gtgtatgaca tttcgttatc gtaccatgaa acaactttaa ccaattggtt
    11461 accgtcaaca gtttgaactt tagtttgagt agcatcaaac aatgaaccgt atgaaatacc
    11521 aacgatatca gatgatacga ttggatcttc agtataaccg tatgaatcgt tagctgctgc
    11581 tttcatagct gcatttactt cttcaacagt tacgtctttt tcaagagttg caaccaattc
    11641 agttactgat ccagttggaa caggaacacg ttgtgcagca ccatcaagtt taccgttcaa
    11701 ttctgggata acaagtccga tagcttttgc agcaccagtt gagttaggaa cgatgtttgc
    11761 agcacctgca cgagcacgac gaaggtcacc accacggtgt ggtccgtcaa ggatcatttg
    11821 gtcaccagtg tatgcgtgga tagtagtcat caaaccttgt ttaacaccaa agttgtcttg
    11881 taaagcttta gccattggag caagacagtt tgtagtacat gaagcacctg agataactgt
    11941 ttcagttcca tcaaggatat cgtggttagt gttgaaaaca actgttttaa cgtcgtttcc
    12001 accaggagct gtgataacaa cttttttagc accattttca tggatgtgtt gttcagcttt
    12061 ttcttttgat gcaaagaaac cagttgcttc aagaacgatt tctacgccat cagtagccca
    12121 gtcaatgttt gctggttcgc gttcagcaga aactttaaca aattgaccgt taacttcgaa
    12181 tccaccttct ttaacttcaa cagtaccgtc gaaacgacct tgagttgtgt catatttcaa
    12241 caagtgtgca agcatgtttg gatctgtaag gtcgttgata cgagtaactt caacaccttc
    12301 tacgttttgg atgcgacgga atgcaagacg accgatacga ccgaaaccgt taataccaac
    12361 tttaactacc attagtgatt tcctccttat gaaaatctca aatattttat tttagggatc
    12421 cccctaacca atgtgaaaag attaacttgt acatatgtgc aaaaatcttt caacacaatt
    12481 attatatctt ttttcgagaa aaattgcaat gaatttacca ctttcaaaaa gaaaaaaaga
    12541 tactctttcg agtacctttc ataagttttc agaaattatt caccagagtt tttcttgatg
    12601 atttcttctt gaactgactt aggaacatct tcgtagtggt caaataccat catgaatgta
    12661 ccacgtcctt gtgttgcaga acgaagaact gttgcataac caaacatttc agcaagtgga
    12721 acaaaggcac gaacaacttg tgtgttacca cgtgcttcca taccatcaac acgaccgcga
    12781 cgtgcagtca cgtgacccat aacgtcacca agattgtctt ctggagctgt aattgtaaca
    12841 agcatcattg gctcaaggat agctggttgc gctgatttag cagcttcttt aagtgcaagt
    12901 gatgcagcaa tcttgaaggc tgtctcagat gagtcaacat catgatatga accatcgtaa
    12961 agtttagctt ttacgtcaac cattggatat ccagcaagaa caccgtttgc catagattct
    13021 acaagtcctt tttcaacggc tggaataaat tcacgaggaa ccacaccacc gacgatagca
    13081 ttttcgaatt cgaatccttt accttcttcg tttggtgtaa attcaatcca aacatcacca
    13141 aattgacctt taccaccaga ttggcgtttg aagaatccac gtgcttgtgt agaagcgcgg
    13201 aatgtttcgc ggtatgatac ttgaggtgca ccaacgtttg cttcaacttt gaattcacgt
    13261 ttcatacggt caacaaggac atcaaggtga agttcaccca ttcctgagat aactgtttca
    13321 ccagtttcaa cgttagtttc aacgcggaac gttgggtctt cttcagcaag tttttgaagg
    13381 gcaataccca ttttatcttg gtcagcttta gattttggct caaccatcaa ttggataact
    13441 ggttctggaa cttcaattga ttcaagaata acttttgatt tttcatcagt caatgaatca
    13501 cctgtagttg tatctttcaa accaacagct gctgcgatat ccccagcata aacagtttca
    13561 atttcttgac gactatttgc gtgcatttgt aagatacgtc cgatacgttc acgtttacct
    13621 ttagatgtat tcaacacgta tgaacctgag ttaagaacac ctgagtacac acggaagaat
    13681 gttaagcgac ccacgaaagg atctgtcatg atcttgaagg caagcgctgc aaatggctct
    13741 tcatctgaag caggacgtgt ttcttcttcg tcagtatcag ggttaatacc tttgattgca
    13801 ggaatatcaa gtgggcttgg aaggtaatca ataacagcat caagcatcaa ttgaacacct
    13861 ttattcttga aggctgaacc acacaataca gggtagaatt caacattgat agttgctcta
    13921 cgaatagcag ccatcaactc ttcgttagtg atttcttcac cttcaaggta tttcatcatc
    13981 aaatcttcat cagtatcagc aactgcttct actaattttt cacggtattc atttgcttga
    14041 tcaacatact ctgctggaat atcttcttca aggatatctg taccaaggtc attagtatag
    14101 atttcagctt tcattttgat taagtcaata ataccacgga aatcgtcttc tgaaccaatt
    14161 ggcaattgaa ttgggtgtgc attcgcttga agacggtcat gaagtgattg tactgaatag
    14221 aggaagtcag caccgatttt atccatttta ttagcaaaaa cgatacgtgg aactccgtat
    14281 tctgttgctt gacgccaaac tgtttcagtt tgaggttcta cacctgattg agcatcaaga
    14341 actgttaccg caccatcaag tacgcgaagt gaacgttgaa cttcaattgt gaagtccacg
    14401 tgtcctggtg tatcgatgat gttaacacgg tgaccatccc attgtgctgt tgttgcagct
    14461 gatgtaattg tgataccacg ttcttgttct tgttccatcc agtccatttg tgaagcacct
    14521 tcgtgtgttt caccgatttt gtggatttta ccagtatagt aaagaatacg ctcagttgtt
    14581 gttgttttac cagcatcgac gtgagccatg ataccgatat tacgagtttt ttcaagtgaa
    14641 aattcgcgag ccatttggtt tctcctatat tttaaattat ttacttcttt attataacac
    14701 tatttataaa aaacggatag gcaggaccta cccgttctaa ttgctaacat tgctatctac
    14761 caaacaattt gtttagtagc tatgataagt ctcaaaaaga gagtaatctt accaacggaa
    14821 gtgtgcaaag gcacggttag cttcagccat tttgtgagta tcttcacgtt tcttaactga
    14881 tgctcctgtg ttgtttgcag catccatgat ttcttttgca agacgatctt tcatagtgtg
    14941 ttcaccacga gcacgtgatg cgttaaccaa ccaacgaagt cctaaagttg tacgacgttc
    15001 tggacgaact tcgacaggaa cttggtagtt tgatccacca acacggcgtg cgcgtacctc
    15061 aagtacaggc atgatgtttt ccatagctgt ttcgaatact tcaagagcat ctgttccagt
    15121 agcttcttta attgcttcaa aagcatcata tacgatagtt gcagcagtac cacgtttacc
    15181 atcaagcatt acacggttga taagacgtgt aacgattttt gagttataca atggatctgg
    15241 taatacttcg cgtttaggcg cttgattttt acgactcatt ttctcttatc ccccttctaa
    15301 ttacccttta ggacgtttag caccgtattt agaacggcct tgtttacgat cagctacacc
    15361 tgcagtatca agtgcaccac gaacgatatg gtaacgtacc cctggaaggt cttttacacg
    15421 tccaccacgg ataagcacaa cactgtgttc ttgcaagttg tgtccgatac ctggaatgta
    15481 agcagtaact tcaataaggt tgctcaaacg tacacgagca aatttacgaa gggcagagtt
    15541 aggtttttta ggtgtcatag ttccaacacg agttgcaaca ccacgttttt gtggtgcaga
    15601 taatttagtg tgaacttttc tgtgactgtt gtaaccaata ttcaaagcag gtgaatctga
    15661 tttttcaact ttagatttac gtggtttacg taccaactgg ttaattgtag gcatctacat
    15721 ttctcctgta agttttttat ttttggttga tatagcaatt ggtgacagcc tatatctgcg
    15781 tgtacttttg caacaattgt cagcacgtct ctgtacactt ttgagagacc aaaagtaaaa
    15841 gtaccgttta tcatagtatc attttacaca tgatgtgtca agcttttatt acttttacaa
    15901 gcaaaaagaa tcaaggatgt caccttgact ctttaatcaa atatattccc cgcttctaca
    15961 gagactctat tattttttac atcaatttca gatactctca gaagagattt ataggccatc
    16021 ttctcacgtt gttcctgagc attttctgca aatacagcga cacctactaa tgtgctatcg
    16081 aattctgaga gtaaactaat catccccgaa acagtacctc cacctttcaa gaaatcatca
    16141 actattaaaa ctcgactgtt aggttttaag ctacgcttag aaagaaacat tttttcaata
    16201 cggccacttg aaccactagc gtagtttact gacacagtag aaccttccgt aatcttcaaa
    16261 tcacgtctca caataacaaa aggaacatct aaaacatttg ctactgcgtt tgctaaagga
    16321 acaccttttg ttgcaaccgt catgacagca tcaatttttt gacctctgta agcgttcgct
    16381 acaatgcgac caatactttg taacattttt ggtgtactga gtagatctga aagataaatg
    16441 taacctcccg gaagaatacg atcgttttca gataaacgct gtcttaattc ttcaacgatt
    16501 tctttcgctt ctgcttcagc aatagttggt gtaaagataa cacctccact tgcaccagtc
    16561 acagttttaa tatctccgat ttgagcttgt tcaaaagctt ttttgataat agcaatatct
    16621 tctgaaatcg aagatttcgc ggcaccgtat ttactagcaa atgtatttaa actagttaat
    16681 gtatagggat tatttattag gtaatttgaa ataacaacca tgcgttcgct acgtcttaat
    16741 ttcatatttt ctccttattc atagcattct ttacatatag tataacataa caaatgctaa
    16801 aaacgaacat tttattttag gtcttaatta ttctacctaa attatttaat gttcagttta
    16861 taaaaagaac ggttatccat ggcaaaaatg cgatttgtca tttcaccttc gttgacacga
    16921 tttaaagctg ttgtcatcat catcatattt gcatcaatgt tatcgatcat atggataatt
    16981 tctgcctcca tgattctagg acgaaccggg cttccatatt ctaattgtcc atggtgactc
    17041 agaataacat ggcgtaacac tgtaacctct tcttttgtat catcaatatt aagctctgct
    17101 agtatttttg ttaattcctc atcaataagt gaaatatgac cgataaggtt acctcgaata
    17161 gtatattctg tattatcagg acccgataac tcaatgacct tggctaaatc atgtagcata
    17221 ataccagcaa acatcaaact tttattaagt tctggataga tatctccaat actatctgcc
    17281 aaacgaacca tcgttgccgt gtgatatgcc aatcctgatt caaaggcgtg gtggtttgtt
    17341 ttggcggctg gataggtaaa aaattcctta ttgtatttac gataaagtgc tcttaccaca
    17401 cgctgccagg ttgcattttc aattttaaag agcatttgtt ccatgtactc tctcacgttg
    17461 tcaacattga ttggaggttt ttctttaaaa tctctaggat cattaggttc accatctttg
    17521 atgtttctta atgtaatttg gttgacttgg ggagttccat tataaacttc acgacgccct
    17581 ttcatatgaa caattttacc ggctacaaac tcttctacat tataggtttg tgcatcccac
    17641 atatttcctg aaatttcgcc agtatcatct tgaaaggtaa aggcaataaa gtctttacct
    17701 gcacgcgttt tacgaacttc tgctttcttt ataaggtaaa acccctcaaa aagctcgtct
    17761 tttttcattt ggttaatttt cattaatttc ctcttcttct gttataggaa ggtttaacaa
    17821 gctagtggtt gcttgatctt gatgttcttc aactgtattt aaggctctga taatagcgtt
    17881 tgtccgcgtt gtgagtaaat tatcaatact tttacttgct gtatttaatt gtttttgagc
    17941 ttttgataac atgccaccaa attttccaaa ttcaaccttg acattcccta aaattttact
    18001 aatatcatta gcattctttt gaatatttaa ggttttaaag ccaacagata atgaatttag
    18061 taaagcagat aaggttgacg gtccagctac tacaatattt tcgtcacgtc ttagactatc
    18121 aaagaatgtt gcatttctta ccacttcaga atagagccct tcagttggta agaacatgat
    18181 accaaaattt gtcgtttcag gaggatttaa gtacttattg tttatatctt ttgcaaattt
    18241 acgaatactt gccagtaaag atttacgata gagttctatt tgaaccttgt cacccaattc
    18301 ataagcatct tccaatcggt aataatcttc tagaggaaac ttagagtcaa taggcaaata
    18361 gatataatct ccctgaccat ttccaggtaa tttaatagca tattcaacac gctcagaaga
    18421 gccagacacc gtaggaaatt ctctctcata ttgactaact gtcataatat cttctataat
    18481 ttggccgagt tgtaactccc ctaatattcc ccttgtctta gtatttgaca agactttgtt
    18541 cagcgtacca acatcttggg caacagtttt catctcccca agaccttgat tgacactctc
    18601 tagttgacgc gatacagttt caaaagaagt ttgcaaacgc gtttgtagcg ttttttctag
    18661 cttttcctca acagtttggc gcatttgatc caagcgtttt tcattggaat cttgcatttt
    18721 ctgaactgac tgagataaat tggagttaat caattccaag cgtttgtctg atcgatctcg
    18781 actctccgtt aaatgctgat ttaattctgt acgcatatcg gtaagcaact gataaatttc
    18841 ctgttgcata cggtttagct gattagtcaa cgttagaatt tgatcttttt gcgccgcttc
    18901 tatctgatat gtcagttggt ctgatagatt atcagcatta tcttcaagtt gtttttcaac
    18961 ggactttgtt aattccgtca ttttttggta agaaattatc gtcactgtca gactagctag
    19021 tacaatgatc aaaatgatga caagcatatt aacctctatc cttactataa ataatgatta
    19081 agtaaccatc tttgagctct acttcaattg gcgaagtcat aaattcatta ctggaataca
    19141 tttttttctt aaaataatta cttttgttca attcatattt agccccagaa atctgcaaag
    19201 tagactcact ctcaggcatg aacgagacat aggacatccc ttcaatcctt gagacttgat
    19261 ttttcccgga tggtaggtaa gtcactatgt tttgttcatc tttgagtctg atttgtgaca
    19321 taaatggttc tagatctgtg tcacttggta aaaagatatt agacatcata tggtcaatcc
    19381 tgccaccaaa tgctccaaat acggtcacct gagcttctgg aaattctttg aaaatagttt
    19441 ttaaagctaa ttctgtatct gtatcatttt tttctgcact agcagaaacg atattgctac
    19501 aatagtgttt gatataaagc agttcatctt ctgttataga atcaaaatca cccactgcca
    19561 tgtccaaaga aagcccattt tttaataaaa ataggcttcc acggtcaatt ccgacaaaat
    19621 agtcaaaatc atattcaaaa taagttaagt caccgccagc aaataaagca attttagtca
    19681 tcgagcgcca cccttaaggt ttctacttgt gcaggtaaat cacttgcttt aaagagataa
    19741 gagcctgcaa caaagacatt tgcaccagcc tctgctgctg ccttaattgt agtattatct
    19801 ataccgccat caacttcaat atcataatga tgaccgtact ctttgcgcca tgctgcaact
    19861 gtctttactt ttgacatcat ttctggaata aaggcttgtc caccaaatcc aggattaaca
    19921 gtcataatta aaatctggtc aaccaaatct aagataggaa taagagattc tacgggtgtt
    19981 cctgggttaa taaccacacc tgctttcatt cctgcctctt ttatcttttg aagcgcacca
    20041 tgtatgtgtt tcgttgcttc aacatgaatg gtcatgatat cagcaccagc ttgagcaaaa
    20101 tcctcaatat aacgttctgg atcaacaacc atcagatgac aatcaaaaac aagtttactg
    20161 tgtttacgca tgctggatac aacatccgca ccaaaactaa tattagggac aaattgacca
    20221 tccatgatat caatatgaac gtattctgct gttgtctctt caatacgttt caattcgtta
    20281 gcaaaattag catagtctgc tgctaatatc gatggggcaa ttttattggt tgacatagaa
    20341 acctactttc ttttaataac tttcttatat gtttcacgac ggttctcaat ttcacttaaa
    20401 aattggaggt agttatcata gcggacttgc cataactgac cttgagtcaa ggcttctttt
    20461 acagcacatt taggctcatg tgtatgcgta cacgagcgga atttacaaaa atgactaagt
    20521 ctacggagct ctggaaagga ttcgtttaag tcctctgcat tgtccacttc atagtccaaa
    20581 gaagaaaaac caggagtgtc tgcaattttt cctttatgaa cattatagaa actaactgct
    20641 cttgtggtgt gtcggccacg gcctaaacta tccgaaattt caccagtttc aagtttcaac
    20701 tcaggagcaa ttttgttgag aagcgttgat tttccgacac ctgtctgccc cataaaaaca
    20761 gtcactttat ctgctaacag aggcaagagt tcctcttgag aataacagaa aacgtagcct
    20821 atattctgat aatgttcgcg aatatcatca ataacaacca aatcgtctaa caaatccaac
    20881 ttggaaatat aaatgatagg ctgaatcatc ttatattcta gaagtactaa gaaccggtct
    20941 aataagttag cgttaaaatc aggttcttta gcagacatga taacgacagc ttggtcaata
    21001 ttaacaatag gtggtcgtac gaggctattt ttacgctctt caattgatag aatatatcct
    21061 tctgattgat cttgcgaaga aaattccacc caatccccaa cgtagggaat ttgacctttt
    21121 tttctaaaat ttccacgcgc acgtgtctga taaacaacac cgtcagattc cacataataa
    21181 aaacctgcta gcgacttgac aattctacct tgcaattgtt ctccttaatt gtttagaata
    21241 ccactattat atcataaaat gagaaataag ctctactctc agagataagc taaatagaaa
    21301 ctatttgcag gtgttcgatt tctctcaaat aacctcaatt ttatgaattc ccaacataaa
    21361 agcagtgatc ttatttagat acactgcgct tttttccaat tactattaag gcacctactg
    21421 taatttctga tatactcgcc caatcaatag tcgttttatc tacgctgaat aacaacggtg
    21481 tcatatcaat aaaagcttga cgttcttgtt gagaacagtt ataactttga acaactttct
    21541 gctcagaaat aatttcaaaa ttttctctaa agtaatctag aattttttga ttagagtaat
    21601 ctttctgttt caagtattga ctagcttttt ctcgcaattc ttggacatgt tcagctacag
    21661 gaacaacctt tacaaggata ccatcatcac tcagtacccg cctaaattct ttataatttg
    21721 caggagaaaa aatgtctaaa atgatatcaa ttgatgagtc ttgaatagga aggttagcta
    21781 aatcagaaac aaaccatttc acaagcctag attgatcact tttagcagct aattgaatcg
    21841 aatctttaga aatatcaaaa gccaagaaag tcttctcata cttgttaact aattgacgag
    21901 aataaaagcc ctctccacaa ccaatatcta aaaccgaatg aaactgagaa ttattttcca
    21961 aaacctttga aatagcttct aaaatatgat tataataccc gttttctaag acaagctgcc
    22021 gattttcaaa tgatttttta tcataatgtt catctatctt ctttcctcca agaagattaa
    22081 catatccaaa tttggataaa tcaaaacaat gattatcaga gcatttgagg ctgttacttt
    22141 cttttatcaa gggattttga catttaggac aagcgaaata acttgcacta tcttcaaaac
    22201 gccacttctt gtcaaccaac atagtttata tcccaacctc tttcaaggca tctgctaaac
    22261 ttgcaaaatc agggatagat aaggcttctc cgcgaataga tggcttaatt tttgctattt
    22321 ctaatgcttt ttccaatttt gctttagtat cttctgattt tccaaaatga ctcgttagat
    22381 tattccaaag tgttttacga cgatgtacga aggctacttt tgacactctg aaaaagaaat
    22441 cctcatcttg aacactcact actggttgat ctctacggac cattttcaaa atagctgaat
    22501 ctacatttgg agcaggaaca aaaacagttc tcggaacgat aaatgatact ttggcagtca
    22561 tataatactg cactgcgatt gacaaagaac cgtatgcttt agtatttggc atagctgaaa
    22621 tacgatctgc cacttctctt tgcatcatga ctacaaactc cgcaaaagga atcttgccct
    22681 caatcaagtg catgaggatt ggtgttgtga tgtagtaagg taagtttgct actaccttga
    22741 taggcaggtc aggattcttg aaagcctgaa tctgggtctg caaatcagcc ttgaggatgt
    22801 cctgattgac cacttggaca ttgtcaaaac gcgcaagcgt atcagcaagg ataggaatga
    22861 gacgatcatc aatctcaaaa gccatcacct cagctgcatt ctcagccaaa aactctgtca
    22921 aagccccgat accaggacca atctcaatca cgttgacacc cttgtcaatc tctgccgtgt
    22981 caacaatctt ctgtaaaata ttagtatcag tcaaaaagtt ctgaccgaat gatttcttaa
    23041 atgtaaaccc atgacgctcc aaaatagcgc gggtaacggt tttatctgcg attctcattt
    23101 gtttctttcc ttattgtctc aagtctatcc taagacatct aacaatttct cttttaattt
    23161 ttctatttct gcaccagttg ctctgacact atctgcatac atcttctcaa aaggatacca
    23221 ccaaactgga ccttttccat tatgcccata gttccttaaa tcacctgttt ttcttggaaa
    23281 aagatgaaaa tgtagatgag cgtctccatt tcccaaactc tcaatattca tcttatctgc
    23341 tctaaaagcc ttagcaacta tttcagaagc atctgccatt tcggaaagat actgatttcg
    23401 ccaatctcta ggcaattcat gtaactctgt tacgtgtttt ttagagagaa aaaggcaata
    23461 atcttgaaaa tattgatggt ctcccagaac aagataaccc gtttcatatt ctttcacaaa
    23521 ataaggattc tcatttcgtt caatcatttc aatacgttga caaataagac acatatttta
    23581 tccctcgtat ttctccatac actcttcaac ttctgccttg gtcactccga agagtctgag
    23641 gcgtttgagg agttgtttac catttgagta gccgatgcga agttggctac ccagatactc
    23701 acgacgtttg cggctatcac tagcggtaat gaacccccag cgaatgaggt cggcttgggt
    23761 gatgtcgaaa tgatcttcat catcgaaatg ctgagtcact tttgagagtg ccttctgcaa
    23821 atcctcaaag ctggcatgct cgacacccag agatcgtccc ttggtctttg aaactggctt
    23881 tgcctcgtct cgattgagga aggcatggcg gactgtcgga atggccttca tgatgatttt
    23941 acggatacgt tcgccattgt aatctgggtc ggtaaagatg ataactcccc gcaaattgtg
    24001 caaacgttcg atacgctcta aatcatcttc atcaattgct gaacctctag tttcataggt
    24061 atcaacattg taaaagcgtc gcagattagc agtatcatct ttcccttcga caacaatcac
    24121 ttcttgaata tcaatttttt tcatcaagtc taaatatcct tactgcattt tggtatgtag
    24181 cctccgctac ctcttccaca gttatgcctc gtagctctgc tattttttca acgacatatc
    24241 tcgtataagc agttttgttt tctcgacctc ttttaggtac aggagccaaa taaggagcat
    24301 ctgtttccac taatatctta tctaatggca actctcgtgc agcttcttgt acatctaatg
    24361 ccttcttaaa ggtcacaaca ccagaaaagg aaatcatcat ccctaaatca ataaatttct
    24421 gagccatttc caaagaacca gaaaaagagt gcattatacc accaaaaggc ccaactccag
    24481 attccttaat gacttcataa gtgtcttcta acgcatcacg tgtatgaacg acaaaaggaa
    24541 ggttatactc ttttgaaagt tcaatttgac gcttaaacac ctcgatctga atatctttgg
    24601 gatcctccat ccaataataa tccagaccaa tttcaccaag tgcaataacc ttgggatttt
    24661 ctaaatggct gataatcatt gactcaatat tatcatcata agaaccagct tcagttggat
    24721 gccatcctat tgtgctatat acctgtgcat attgggaaga taattccaaa gattttgaaa
    24781 tggtatcttg gtcaaagccg acaacattca tttttgtaac accgagttcg ctagctaaat
    24841 taatttcctc gtctatcttt ccttcaaaat tttccacgtt aaggtgggta tgggtatcaa
    24901 atatttttat catatcattg ttctttcttt gcttatcgta gctacaagca ttaatcttta
    24961 ttattataac ataagactga agcattctga tcagaaagag taaatcaaaa aagtcaaaac
    25021 atttttgctc tgacttttga atacttgctg taaaaatcct ctacaagtgt ctagctaatg
    25081 gtagagaaac tagactataa atgattatat ttttgctgat tgctttcaat agactcctca
    25141 acctttgaac gaaattctcc ttttattgcc tcgtccaatt gattagcaac tttatgaata
    25201 gctgtctttg tgttgatggc tacttcgtct atttctttat gatgcgacgt aataatatcg
    25261 tgaaacttag atctcttgcg tggcgcgtac acgctatgta tcagttcttt atccaattgt
    25321 ttttttaaag tccttactga ctccttacca gtcaggacat cccacattaa actcaataat
    25381 ttctcctact tcattaattc tcttttcttt tccctttgca actcttcaaa ggttagttca
    25441 tgttctgaac gttgccgaat atagttagct ctatattctt cttcttgagc ggacaaggca
    25501 tagtactctt tatggtaagc ttgctctaag tcgtcatgat aagaatctaa aatacgattg
    25561 agcgtcatca tatctgagct atcattacta tatctcaaat catattcaac agcgtcgtaa
    25621 gtttcttgaa gcatattagt aatcttattt tttcttactg agagatcgtc taatttttct
    25681 tcaaactggc aacaactatg gtgataggca tcatctaaat ctgatatttt tctctgagta
    25741 tcagctaatt gtttttcaat gatatcaatc agtgccattg tttaaactcc cctgacaagt
    25801 tactatcttg ttgcaacata gcttcaaccc ctttattaat ttggttttct aattcaacgt
    25861 aagcgtgaga aatgctttct gccgcttgaa ttttatttga gaaaaaggtt tgagcttctc
    25921 cgacaatgct cgcatagcta acacctgact gagcatacgc ttcttccaat tcttgaggac
    25981 ttagttcagt aactcctgta ggagctactt ttaaagattg ataaagctct tctgctttag
    26041 caactgcttt ttgctcaact tttttaactt cttcaactgc ttcttttgat atcgttacca
    26101 aaccactaga aatcactttt gcttgttgac tatctaggaa aatagcttga ttcttactaa
    26161 cttcttttcc cgtactttta aagctactaa gtttcgcttt aatagcatgc attctttgat
    26221 tcacattcgt atgaactact gaatagtttt ctttgtaagc aaatttatct tctgctaatt
    26281 ttaaagaacc atcttgattg tactggtagc ctccccaaat gtgctgattg gtaaaattag
    26341 caacccagct acctcctgaa acagctttac tgtcaactat tttaacaatt ccgacagcat
    26401 tatcgcttcc ttctttagga taacctattc caattttatc cttggaatca acgtaattat
    26461 tgactacccc ttttatttta tcaacctgtt ctttttgttt atctgtcaaa atactataaa
    26521 ggtttggacc gttatatatc caagctcctt gaactcggta aatttgatta tcatccaatt
    26581 ctgcaatagc atactgtgca ttcattgacc ctaacgagtg tccgtagacc gaaatttttg
    26641 cattaggata tttattcata acttctttta gcagatgagc tgattgttct aactgctttg
    26701 taccttgttt atgagataca ctttgtgaac caaatttctt taataatagt ttactagcta
    26761 ttggaatatc atttttaaac cagtctgttt tccaatcatc tcctccaaac ggtgctgttg
    26821 accccttaaa taaaaccgta acttccttaa catcctctgg cttgtctaaa gttccatttg
    26881 gagtgatggt ataaatttgt tccccagttt ctttgcctcc atcttgaacg tgagcaactt
    26941 taccgatggg aagtacctta tcttggctca ttattttgac tagtgtatca agttggctac
    27001 ctaattcata ctcttgcttc gctatattta ccctatctaa atcagtcaag actttttcca
    27061 ttaatgacct ctactttcca ataaatgagt taactgggga gattgatggc tactttgact
    27121 gaccaaacgc ccttgattat ccttatccaa attacaagta atatataagt taggatcttt
    27181 attaatgatt aaccttaccg aaatccctcc cataggattt tttttgatac tttctttgtc
    27241 aatatcatac gacttaataa cagcattctc tttgaaagcg tctttatctt ctgcataaat
    27301 ccacttttga taagcatttt tagcgtcttt gctattagct atttcccata attcttgttg
    27361 tttattgttt tcagtcactt ttaaacctcc tacagtgaaa ccaagcccta aaaggattat
    27421 tacaataaaa ctaagtaatt ttattgactt catctctcct ccttttatca tataacactc
    27481 atttgatatc tagtttatca taaatttatt ctcattataa acgaaaaact ataaaacatg
    27541 gattagttat cttaggatta ccaaatacaa agatattaaa aaagatgatt aatagctcta
    27601 atgctataaa tcatctgact ctactattta cttgtaatca cttggacgtc ctgtatattt
    27661 tgcccaagat ctcttcaaaa atttattatt aatgtgataa tttggcttgc tgttttcctc
    27721 tgttaaaaat ggattgatat gcttatctag ctctaaccaa ccattccaac ctaaatggat
    27781 tgtatcttgc ataaagtatg gcttacctcc atctcgagaa aggtcagaga tatgattgaa
    27841 accttgactt tgaagttggt gttttatttt ttctacagat ttttgataca ttttttgatc
    27901 aagccctgtg tagtctgtcc actttttatt tactggtggt atgacaaaaa ggacatctgt
    27961 attttgtttt gagaattcat taagaaccaa ctgtaaatca ttaaattctg gtgacttagt
    28021 ataattgaaa tgtcgttgtg accctttcaa tttttttaag tgctttttaa ttcttgtttg
    28081 gtaaaagcga tcatcaatac caaattgatt tgatctcgtt gctactttag catcttgtga
    28141 tgcaatacgc gataaatgat tatatgagaa tgctttcggt aatttaccaa catgaggcat
    28201 tattacctga ttataagttt ttcctagtgg tagatagcta aaaatagcat ctgtcttctc
    28261 aaatacccaa tcattaaact ttagtctttg tttgtcttta tttgatagtt tttggccatc
    28321 tgcaactttt tctatcaaat ctgacataga agcttcagga tataatttta acaatcgacg
    28381 agcagcatac ctatcgtagg ttgtccctgt ttgattttgc aaaaatcgaa tggtttgatc
    28441 gttgctgaaa tagttttgaa aagcaccttt gttggctccc ttgcgaacaa accattgcgg
    28501 agaaataaca tagatagctt ttttattttt gatttgtcct ttaatctgtt gaatgccata
    28561 gtattgtgtc agagaagccg cccctttttg acctaaaaga tatgggatat aaggcctttt
    28621 gtaagcctct gctaaaactg atggatgcat tgcatcaaat cttaaccact cacttgaacc
    28681 aaagaaaggg acaaaattgg ctttaggatc actgaaagct ctaacttttt gaactctgct
    28741 tttaaaacct gcctttgtca acgctactgc actgtttttt tccttaccta aatgatgagg
    28801 ctgagaaaca ggaaaaacaa gatataatcc taccaacaat agtaaagcac aaactagagg
    28861 tccaaatact ttacctagac gtttaagcat tgcagagctc cgttactcca gcaacaattt
    28921 tattagctgt gttccagtca ttacgaccaa attccgcaat cggaatatca atattaaagt
    28981 ggctttccaa ctcaacaatt agttcaacag tacccatgct gtctagaact cctgcgtcaa
    29041 ataagtcttc atccatcata gaagaaacat cctccataaa taggtcatca ataattgcta
    29101 atacttctga tttaatatcc attataatac tccttattat ttgttatgcg gttgtgggaa
    29161 ccacaaaaga tctaaaaatc ctgaaaaaat caagaaagaa aacataacta cattaaaggt
    29221 gatgaagaca cccaaagcat aagtccattt gttttctggt aaaagtggtt ttttagccaa
    29281 tcttctttct ttattaatat ttttcttctt acgtacccaa gcgtcattaa taactaggcc
    29341 aatcccatga aagagaccat aggctatata gtaccaagtt aacccatgcc agaatcccat
    29401 aagaagcata ttgataatat aagctacact tgaagtaaca ttacggtttt taaaaacttt
    29461 atttttaact aaaagcttaa caagcctcat gaaaacaaag tctctaaacc agaaagaaag
    29521 gctcatatgc catctattcc aaaattcttt taggtcgcgt gatttgaaag gtttgtcaaa
    29581 gttaatcgga gacttaatcc ccattaggtt agaaatagcc aacgcaaaca ttgtgtaacc
    29641 tgcaaaatca aagaacaaat caaaaccaaa ggcatacata accccaagtg ttggccaatt
    29701 aaacacacca ccctgttcta gtgctaattc ttttagagga ggcataatca gacttccaaa
    29761 aatataggct aaaataaact tatagagaaa acctaacatc aaataatgaa tcgcttgacc
    29821 taacatatct aggagttcat ttcgatctgg gatattaatg taatcctcat tgaatcgttt
    29881 aaaacgatca attggtccac ttgagaaagt tggaaagaag agtaaaaatc ttaagaattc
    29941 ccataaagta aattctttca agacaccgtc tctcatttca ataatcatag caacagctct
    30001 aaaagtaagg taagaaattc ctagaaaccc aaaaagtgac tgatgcccat cctcagatat
    30061 agctggaatt aattttacca aaaatagcgg aaaaatagat aaacaaaccg ttacataaaa
    30121 gaccaaagta tgattaaatc gtttccggta agctttatag ataaaaacta gcagagattg
    30181 tccgacaacg tatgccaata gtgacttcaa ttgattaaaa gtactaccag tcaacatcat
    30241 tacaataaaa cttagactaa aaataatctc atataaggca aaacgcttct taaagaataa
    30301 gccgatgtaa attggtagaa cagctaagat aaggtagaag aaatattgtg gattcccata
    30361 aacatccaag tgaggaagtt tttctaaaaa tgcctgtatc acttgtttac ctcgctcata
    30421 agacctttga tatcaatttt tccatttggt gtcaaaggta aatcttctcg atagataaat
    30481 ttagatggca tcatgtaatc catcataatg tcctttaagt cttccttaat tgcttttgtc
    30541 aaatccaaat cacgttcaaa atcatctctt acaccttctt ttaagacaat ataggctaat
    30601 aaattttgaa ctttatgatc cttgttataa cgtggcactg ctactgctga ttttacatac
    30661 tgcgatttat ttaagttttg agaaacatct tctaattcaa tacgatagcc gttaaattta
    30721 atttggaaat ccatacgccc accgtaaagc agaagacctt catcggtcat acttcctaag
    30781 tcaccggtat ggtaagcagg tagaccttcg aattggaaaa atgcctcagc tgtcttttct
    30841 ggattgttaa gatagccttt tgatactgct ggtccagcaa taatgatttc accttgctct
    30901 ccgtttggta atttatgacc ttcttcatca atcacatacg ttggtgagtc atctttagtg
    30961 taaccaattg gaaggcgttt gcatgtttct aacatttcat cagtaattgc tactgcggat
    31021 agagcaactg ttgcttctgt tggcccatat gcattaacga tacgagcttt tggaaaacgt
    31081 tgacgaagtt tttgtgcagt cttgacagtt aactcttccc catcaaaata aaaatgtgtt
    31141 aattgtggca aggtctctga attgaaatcg ttagatagta gcgccatatc agcaaatgat
    31201 ggtgtcgaag tccaaacctg tattggcaat tcattaatgg tagcgaatag ttttttgaaa
    31261 tcattaacca ctgtttttgg tagggcgaac agggtgcctc ccatagctag tgttggtgcc
    31321 caatacataa ctgataagtc aaatgaatat ggcggttgag ccaacatttg cggtctttca
    31381 ggaactgaaa actcatcatc agaaatcatc caatttgtaa agctcaataa attgtcatgt
    31441 gaaatttgca caccttttgg taaaccagta gtccctgaag tgaaaataat atagtaatta
    31501 tcatcacctt taacagaatg tgttacctca taaggagtct tttcttcaaa aatagctgaa
    31561 acttgagaaa cgtctaggat tgggacatta tcaacttcaa gaggaaattc accaattgaa
    31621 atgataaggc ttggttgagc aactgtcata atagcctgta ttctatccaa agcagagtgt
    31681 tggtcaaccg gtatataagc atgccctgac tttgttaaag caacaaatgt cgccaacatt
    31741 tcatattctt gaccaccgaa tactaagaca ggtgattttt caacaagacc taggctatca
    31801 atatgagcag ctagagagtc agagtctact ttaagttgtc cataagtatg gacttcccct
    31861 aaaatatcat acactggaaa atcagcttgt gtctcagcaa aatgctcaat tgttttaatc
    31921 atatcatgta tcatttgtac catctcctag aattcattat aaataaaact gccttgtccg
    31981 cgaccaaggt atgcaaaaaa gtaaattagt aacaataaaa taccgaagta taataacgtt
    32041 ctaccaataa aagataaata tggtttcatc attcttcctt taattaatca tggtattggt
    32101 ccaaaatcca tcaattggta actggacttc aagacgtaat tggtcttttt cttgatatgc
    32161 tctaattctc cccttatggc gttccacgat tgccttagca atagataagc ctagaccaaa
    32221 actgagagaa tctgcatgat catctttagc ttgataaaaa cgctcaaaga gattatccat
    32281 aacctctttg gaatagatag cacttggatt tgatattgtc agaatagctt ttcttcgagc
    32341 ctgtttaaga ctaaactgga tttctgattt tgacaaagaa tgcctaatag cattatccaa
    32401 aagaattatt aaaatttgct taatctgact ctcttctcca acaatccaga catcatcttc
    32461 aatcgtatca tttaaacact tttcttcttc atatgccaaa ctttcatatg tcagaagtac
    32521 ttcttgacat aagacagata agtttaactt tttgaaattt accttggagc gtttttcatc
    32581 aagtctagac aaattaagca aatcttcaat taaaaactcc attcgcttag tctccgaaac
    32641 aacatgatcc agataacgat ttcctggaat ttgctgttcc agaacctgaa cgttggctcg
    32701 tatagctgca ataggggtct tcagctcatg actcgcatct gaaacgaact gtttttcgcg
    32761 agtcatttct gtctctacag gccctacgat aaatcgagaa agataaagac ttacagctgc
    32821 taataaacaa aatccaaaaa tagtaaaaac aagaattaga aaacgccttg cataagggac
    32881 ttcataacta gccaaatcca taatagctat ggtgtaggtt ttatctttat attcttttat
    32941 ggaatatata aaagacttcc attttcgtga cttctttcct tgctcaagga gcttcaaact
    33001 agatttttcg ataacatcat ttgtcaaatc agtattattg ctttgaatct taaccatctt
    33061 ttggttatct atagtcacca aagcaatcgg ttcatcgcct ggtattccta aataatcatt
    33121 tttggcaact aatttaacaa tcctatatgc atcatattca tcccaatatt gattgtagcg
    33181 attactcact gcaaagagaa agagaacaac gacaataagg attgacatgg tcaaaaagac
    33241 aaattttttg cgtaaatcac taaacattat gtcctctcct ccaaggaata tccaaagccc
    33301 ttagtggtta ttatttcaat gtctgctttt aaaaagcgca atttttttcg caaaaaagaa
    33361 ataaatactt ctaactggtt atattctgaa ttgtctgaag gtccccaaac cttagaaatc
    33421 aactgatttt taggaataat ctgctttgca tttcgcataa aaacttccat caagagaaat
    33481 tctttattgg ataattttac actactttct ttagattcta gctcatgtga atctgtgtta
    33541 agccttatat ttccaagccg caattgattt gctctgatta gagaagattg tctggttctc
    33601 agcctaattc tagctagcaa ttcatctgct tcaaagggtt ttgtgatata atcatctgcc
    33661 cctaaatcta aaccattaac tttatcataa gtctgtgatt ttgcagttaa aaagattatt
    33721 ggggtttcta aaccagcttc cctcactctt tttaaaacag ataatcctga gcgtttgggt
    33781 aacataatat ccaacaggac taaatcatag agacctgtca aaatgtattc taacccatca
    33841 tctccatcaa atgctaaatc aacagaataa cctttatctg ccaataaagc ctgaatagcc
    33901 tctgctatac ttttttcatc ctcaactaca agaagtctca ttttctagtc accttacctt
    33961 atatattata gtataaacta gcaaaattga aataaccttg aaaaaacgat attaataaaa
    34021 gtatttccta agtcactaaa ctacttcaat aatacaagcg tctaaaaaat aaaatatcac
    34081 tcgctataaa gatatttctc aataagaaat ctcctcatat tttaacaaaa ataggggcta
    34141 tttgtctacg aatttccgca ttataaactc acttttcatt aactttatct ataatatact
    34201 caaactactc tcacaaaaac ctttatcgtt atgaaggtac caaaaaagcc accagactcg
    34261 agtacctgtt ggcttgatat tattttgcta tctatattaa gctgataaca ctttacgtcc
    34321 tttacgacgg cggctagcaa gcacgcgacg tccgttttta gttgacatac ggtgacggaa
    34381 tccgtgttta cgttggcgac ggattttact tggttgataa gtacgtttca cgttgaatac
    34441 ctcctgatag attttcgtat tcgtttagcc ggctattttg atcagttact aaacatacca
    34501 tactatttta taacaatttt aggcctcttg tcaagaaata ctttcaagtt catttgtctt
    34561 ttttcagtaa atagtttcat ttgttagcaa gaaatgcttc tctattttct ccaactaaaa
    34621 gaaaagatgt gccatcaaaa caataatagg taatgaaata attgttctct ctataaaaat
    34681 gataaataat tctagaatgt tgatagggat cttggaacct aatatcaccg caccagtctc
    34741 agacatataa attaattgca caatagaaac tgttgcgaca ataaaccttg tcatctcact
    34801 gtgaatttca gccgccatta tcgaaggaac aaccatatct gaaaaaccaa caatcatggt
    34861 ttgactcgca atatctgctt ctggaatccc taaaagttca aggaatggaa ggaatggtgt
    34921 acctaaaatt ttaaaaatag gtgttgatac tgaaataatc aaagctattg ttccgacagt
    34981 cataattgtt ggcataacgc caaaccataa actaagtact gtcttagagg cattattgac
    35041 aaaagtcttc atgtttccat ttgcttttga tttatcgatg gcaagcttaa gtccccactc
    35101 tatcttgtta taaccatccg gaattacctc agatgtttct ttctttggtt tagtgtaata
    35161 actatttgat tgacgagata agggagggat ataagggata atggatgcac agacaacact
    35221 gctaatgatg actgttagat aaaaatggcc aaaataatca gttaagccaa cattctgcaa
    35281 aacaacaaga caaaaagtga ttgatactgc tgaaaaagta gtggaaatcg ttgcagcttc
    35341 tctactggta taataaccct caacatactg cttatcggtc aaagcaattc caatagtacc
    35401 atcacctaca aatgaggcta cacaattaac tgctgaccga ccaggtagct gaaagaatgg
    35461 acgcataatg ggggttaaga agacaccaac aaactccagt agaccaaatt ctgtcaaaaa
    35521 tggaagaata aaacccgcag caaggaaaat ggtaaacaaa ccaccaatca aatcaaaaag
    35581 gataaggccg cctgtctctt tcccccatac catttcagga ccaatatgta aaaccgtcat
    35641 cagaccaagt gccaaaccga tcaaacgaat aatcagccag aaaatggtaa tgtcagaaat
    35701 ttcttttagc aagtcatttt tttcaataaa gctaggtcta aacagacgat aacaaagtgc
    35761 taacacacat gaaataaaaa tgattagtaa aataatataa tgtataggaa aaacagcgtt
    35821 aatccattga ttaattgtct tagagattac agagacagct actgttgtca taccattata
    35881 tttaaatgga gtcattaata ataatactcc aaataacg
//
LOCUS       DATRTN010000012        35855 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.012, whole genome shotgun sequence.
ACCESSION   DATRTN010000012 DATRTN010000000
VERSION     DATRTN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35855
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.012"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            52..1386
                     /locus_tag="VBE22_000638"
     CDS             52..1386
                     /locus_tag="VBE22_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO7202112.1"
                     /translation="MEDPGSQSLLLQFVVLLILTLFNAFFSASEMALVSLNRSKVEQK
                     AEEGDKRYRRLLDVLENPNNFLSTIQVGITFISLLQGASLSASLGHVISGWLGNSATA
                     RTAGSIIALIFLTYVSIVLGELYPKRIAMNLKDRLAIVSAPIIIFLGKIVSPFVWLLS
                     ASTNLLSRITPMTFDDADEKMTRDEIEYMLTNSEETLDAEEIEMLQGIFSLDEMMARE
                     VMVPRTDAFMIDINNDAQSNIEGILSQNFSRVPVYDDDKDRVVGVLHTKRLLEVGFKT
                     GFDTIDLRKILQEPLFVPETIFVDDLLKALRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDTAEQFVREIDENIYIVLGTMTLNEFNDYFETELESDDVDTIAGYYLT
                     GVGSIPNQEEKVAYEVDCKDKHITLINDKVKDGRITKLKVLLSDIEQNIEKD"
     gene            1454..2242
                     /gene="pflA"
                     /locus_tag="VBE22_000639"
     CDS             1454..2242
                     /gene="pflA"
                     /locus_tag="VBE22_000639"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEO7202113.1"
                     /translation="MAEIDYKKVTGMIHSTESFGSVDGPGIRFIIFMQGCKMRCQYCH
                     NPDTWEMETNNSKERTVEDVLKEALRYKHFWGKDGGITVSGGEAMLQIAFITALFIEA
                     KKLGIHTTLDTCGFAYRATPEYHAILEKLLDVTDLVLLDLKEIDPEQHKIVTRQSNKN
                     ILQFARYLSDRGTPVWIRHVLVPGLTDIDDHLKRLGEFVQTLDNVDKFEVLPYHTMGE
                     FKWRELGIPYPLAGVKPPTPERVKNAKDIMKTESYTEYLKRIQN"
     gene            2359..3294
                     /locus_tag="VBE22_000640"
     CDS             2359..3294
                     /locus_tag="VBE22_000640"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEO7202114.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAWGLDTEAVALGTPN
                     EETAYVLDYFGVQAPRVVESAKAEGVETVILTDHNEFQQSISDIKDVTVYGVVDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRMFKENGVSVPKELAGLLLSGLISDTLLLKSPTT
                     HASDIPVAKELAEIAGVNLEEYGLEMLKAGTNLSSKTAAELIDIDAKTFELNGEAVRV
                     AQVNTVDINDILARQEEIEVAIQEAIVTEGYSDFVLMITDIVNSNSEILALGSNMAKV
                     EAAFEFTLENNHAFLAGAVSRKKQVVPQLTESYNA"
     gene            3354..4007
                     /locus_tag="VBE22_000641"
     CDS             3354..4007
                     /locus_tag="VBE22_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEO7202115.1"
                     /translation="MILTFNPGKLERQEFFKELINYLWIHDDVTLRQIKSHFTDYSKI
                     DRLLEEYINHGYILRQNKRYSLNLPFLSSLDGLVLDDLVFIDSDSQIYQLLQKRKFVT
                     NLDNQTNHLVFVEETDFERNTLTLSNYFYKLTNGYPLSREQKKLYQLLGDVNSEYALK
                     YMSSFILKFLRKDSVKQKRTDIFIQALELLGYISLNQDTTYRLNAKLDVEALKIYLT"
     gene            complement(4037..5038)
                     /locus_tag="VBE22_000642"
     CDS             complement(4037..5038)
                     /locus_tag="VBE22_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO7202116.1"
                     /translation="MIQKNKAPFVLISSVIILLLLILVSISLGYANTSVINVLKLISG
                     KSDDAFLFIITNIRLPRIIVCIFGGASLGIAGLLLQTLTKNPLADSGILGINAGAGLV
                     IALTIGTFNVSNPTILYFLPLFAMFGGLVTIFLVYLMSYRRNHNISPTRLIVTGIGIS
                     TMISGIMISIISQSNSQKMDMIVEWLSGKITISSWTTIITFIPILILLWGLTYSRSRH
                     LNIMNLNEQTALALGLHLKKERIYTLMLASSLAAISVVLIGNITFIGLLAGHLSRRLL
                     GNNHKIILPSCLLIGAIILLVSDTIGRLLLVGTGIPTGLVVSIIGAPYFLWLMTKVD"
     gene            complement(5035..6060)
                     /locus_tag="VBE22_000643"
     CDS             complement(5035..6060)
                     /locus_tag="VBE22_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO7202117.1"
                     /translation="MFKASKKLVQKNKSNHFWLVFFITLILFLIGCYASLRFGAINFK
                     TSDLITVLKNPLKNSNAQDVIFDIRLPRIIAAILVGAAMSQAGAIMQGVTRNAIADPG
                     LLGINAGTGLALVVAYAFLGSMHYSTILIVCLLGSVISCLLVFTLSYTKQKGYHQLRL
                     ILAGAMISTLFTSVGQVVTLYFKLNRTVIGWQAGGLSQINWKMLIIIAPIIILGLLIS
                     QLLAHQLTILSLNESVAKALGQKTQLMTAFLLLIVLFLSASSVALIGTVSFIGLIIPH
                     FIKLFIPKDYRLLLPLIGFSGATFMIWVDLSSRIINPPSETPISSIISIVGLPCFLWL
                     IRKGKNL"
     gene            complement(6076..7008)
                     /locus_tag="VBE22_000644"
     CDS             complement(6076..7008)
                     /locus_tag="VBE22_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7202118.1"
                     /translation="MKKIGIIVLTLLTFFLVSCGQQTKQESTKTTISKMPKIEGFTYY
                     GKIPENPKKVINFTYSYTGYLLKLGVNVSSYSLDLEKDSPVFGKQLKEAKKLTADDTE
                     AIAAQKPDLIMVFDQDPNINTLKKIAPTLVIKYGAQNYLDMMPALGKVFGKEKEANQW
                     VSQWKTKTLAAKKDLHHILKPNTTFTIMDFYDKNIYLYGNNFGRGGELIYDSLGYAAP
                     EKVKKDVFKKGWFTVSQEAIGDYVGDYALVNINKTTKKAASSLKESDVWKNLPAVKKG
                     HIIESNYDVFYFSDPLSLEAQLKSFTKAIKENTN"
     gene            complement(7032..7826)
                     /locus_tag="VBE22_000645"
     CDS             complement(7032..7826)
                     /locus_tag="VBE22_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202119.1"
                     /translation="MSHIKAENIIVSYDQKEIINNLSLSILNQKITTIIGANGCGKST
                     LLKALTRIHKIKDGTITIDGHDIAHLPTKEIAKKIALLPQVLEATEGITVYELISYGR
                     FPHQKYLGNLTNDDRSKIHWAMEMTNVAQFANRDVDDLSGGQRQKVWIAMALAQDTDT
                     IFLDEPTTYLDMNHQLEVLELLKKLNNETQKTIIMVLHDLNLSARYSDYLVAMKTGKI
                     IYEGSPSQIMTKDIIKDIFKIDAHIIQDPISKQPVLLSYQLSTDQN"
     gene            complement(7981..9435)
                     /locus_tag="VBE22_000646"
     CDS             complement(7981..9435)
                     /locus_tag="VBE22_000646"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEO7202120.1"
                     /translation="MITIDKILEILKNDHNFREILFHEHYYYNWTQNVTFNALSYDSR
                     QISSDTLFFAKGATFKKEYLDSAITAGLSFYVSEIDYGADIPVILVNDIKKAMSLISM
                     SFYNNPQNKLKLLAFTGTKGKTTAAYFAYHMLKVNHRPAMLSTMNTTLDGKSFFKSHL
                     TTPESLDLFRMMATAVENQMTHLVMEVSSQAYLTKRVYGLTFDVGVFLNISPDHIGPI
                     EHPTFEDYFFHKRLLMENSNAVVVNSQMDHFNIVKEQVEYIPHDFYGDYSENVITESQ
                     AFSFHVKGKLENTYDIKLIGKFNQENAIAAGLACLRLGVSIEDIKNGIAQTTVPGRME
                     VLTQTNGAKIFVDYAHNGDSLKKLLAVVEEHQKGDIILVLGAPGNKGQSRRKDFGDVI
                     NQHPNLQVILTADDPNFEDPLVISQEIASHINRPVTIIIDREEAIANASTLTNCKLDA
                     IIIAGKGADAYQIIKGNRDNYSGDLEVAKKYLKR"
     gene            9559..11193
                     /locus_tag="VBE22_000647"
     CDS             9559..11193
                     /locus_tag="VBE22_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO7202121.1"
                     /translation="MSQKTTKVSQQEQMVKGTAWLTAGNFISRLLGAIYIIPWYAWMG
                     KHAAEANALFGMGYEIYALFLLISTVGIPVAVAKQVSKYNTLGKEEMSIYLVRKILQF
                     MLILGGVFALIMYIGSPLFASLSKGGQELVPILRSLTLAVLVFPSMSVLRGFFQGFNN
                     LKPYAISQVAEQIIRVIWMLLTAFYIMRLGSGDYIAAVTQSTFAAFVGMFASIAVLLY
                     FLWRYNMLSALIGKTPEHIKLDTKEILIETVKEAIPFIITGAAIQIFKLIDQFSFGNT
                     MALFTNYSSEELRVMFAYFSSNPGKVTMILIAVATAIAGVGIPLLTENFVKNDKKAAA
                     RLVVNNLQMLLMFLLPAVAGSVILAKPLYTVFYGLPQGQALGLFVISLIQTIILSIYT
                     VLAPMLQALFENRKAIIYFLYGLVAKVILQVPSIFLFHAYGPLFSTTVALCIPVILMY
                     LKIHEITGFKRQAIRRTSALVLILTLLMSFIISMIIWLMNLVIVPDSRLVSLVYIIVI
                     GAIGLGVYGFMALATHLLDKMIGSRAQDLRRKLHLN"
     gene            11329..12549
                     /gene="pepT"
                     /locus_tag="VBE22_000648"
     CDS             11329..12549
                     /gene="pepT"
                     /locus_tag="VBE22_000648"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEO7202122.1"
                     /translation="MSYEKLLERFLTYVKINTRSNPNSTQTPTTQSQVDFALTVLKPE
                     MEAIGLKDVHYLPSNGYLVGTLPATSDRLCHKIGFISHMDTADFNAENITPQIVDYKG
                     GDIELGDSGYILSPKDFPNLNNYHGQTLITTDGKTLLGADDKSGIAEIMTAMEYLASH
                     PEIEHCEIRVGFGPDEEIGIGADKFDVKDFDVDFAYTVDGGPLGELQYETFSAAGLEL
                     TFEGRNVHPGTAKNQMINALQLAMDFHSQLPENERPEQTDGYQGFYHLYDLSGTVDQA
                     KSSYIIRDFEEVNFLKRKHLAQDIADNMNEALQSERVKVKLYDQYYNMKKVIEKDMTP
                     INIAKEVMEELDIKPIIEPIRGGTDGSKISFMGIPTPNLFAGGENMHGRFEFVSLQTM
                     EKAVDVILGIVAKD"
     gene            12578..13069
                     /locus_tag="VBE22_000649"
     CDS             12578..13069
                     /locus_tag="VBE22_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEO7202123.1"
                     /translation="MIKLFGKIRYHWQPELSWAIIYWSIAIAPIFIGLSLLYERTEIP
                     SQVFVLFAIFIVLVGIGFHRYFVIEEDGYLRIVSFNFLRRSKFPIEDIAKIEVTKSSV
                     TIKFNNNHERIFYMRKWPKKYFLDALAIEPKFKGEVELLDNLIKMDYFECYRYDKKAL
                     TKS"
     gene            complement(13056..13253)
                     /locus_tag="VBE22_000650"
     CDS             complement(13056..13253)
                     /locus_tag="VBE22_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEO7202124.1"
                     /translation="MKVALIPEKCIACGLCQTYSNIFDYQDDGIVKFSDTDNLEKEVP
                     NSDQDTVLAVKSCPTKALTIL"
     gene            13299..13823
                     /locus_tag="VBE22_000651"
     CDS             13299..13823
                     /locus_tag="VBE22_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO7202125.1"
                     /translation="MAKKPWEKKVVENNSHRKDKITRTSRGVVSSTPWLTAFLSAFFV
                     IVVAILFIVFYTSNRGEDRAKETSGFYGASSQKVNSSKTKKASTSKKTTSSSKTKTSD
                     KSTTSSSTVASSSETSTSSSVNGGTIVVQPGEGAASIAARAGISVARLQELNPGHMTS
                     GYWYANPGDPVVVN"
     gene            13834..14517
                     /locus_tag="VBE22_000652"
     CDS             13834..14517
                     /locus_tag="VBE22_000652"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEO7202126.1"
                     /translation="MNSINIAIDGPASSGKSTVAKIIAKNLNYTYLDTGAMYRCATYL
                     ALQHGYEAQDVSKILGLLAERPISFGKAEDGSQTVFIGTEEVTLAIRQNDVTNNVSWV
                     SAIPEIREELVNQQRRIAKDGAIIMDGRDIGTVVLPDAELKIFLVASVDERAERRFKE
                     NQEKGIESDFETLKSEIAARDYKDSHREVSPLKAAEDAIEFDTTGVSIEGVVTFIQEK
                     AEKIIDMKN"
     misc_feature    14538..14663
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14678..15208
                     /gene="infC"
                     /locus_tag="VBE22_000653"
     CDS             14678..15208
                     /gene="infC"
                     /locus_tag="VBE22_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEO7202127.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQAIADD
                     ANVDLVLIQPQATPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPIPDKK"
     gene            15248..15448
                     /gene="rpmI"
                     /locus_tag="VBE22_000654"
     CDS             15248..15448
                     /gene="rpmI"
                     /locus_tag="VBE22_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEO7202128.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKATMVSSGDFKRIKAMLTRLK"
     gene            15506..15865
                     /gene="rplT"
                     /locus_tag="VBE22_000655"
     CDS             15506..15865
                     /gene="rplT"
                     /locus_tag="VBE22_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEO7202129.1"
                     /translation="MARVKGGVVSRKRRKRVLKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLGK"
     gene            complement(16106..18250)
                     /locus_tag="VBE22_000656"
     CDS             complement(16106..18250)
                     /locus_tag="VBE22_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEO7202130.1"
                     /translation="MKKIKDFASRAINTRLGFILLLVVIYWLKTIWAYHTDFNLGLEN
                     SYQLFLTIINPIPLGLLIIGLALYVKRTKAFYITAFITYAIVNILLIANAIYYREFSD
                     FITVSAVLASSKTSAGLGDSALNLLRIWDLVYVFDFIILIFLFATKKIHLDDRPFNKR
                     ASFSITALSGLLFSINLFLAEIDRPELLSRGFSNTYIVKALGLPSFSIYSGNQTYQAQ
                     KERNGATAQELATAKKYVAEHYAKPNPEYYGIGKGRNVIMIHLESFQQFLIDYKLNVD
                     GKEHVVTPFINSLYHSKETISFSNFFHQVKAGKTSDAETLMENSLFGLSSGSFMVNYG
                     GENTQFAAPHILAQNGGYSSAVFHGNVGTFWNRNNAYKQWGYDYFFDSSYFSKQTKDN
                     SFQYGLNDKYMFADSIKYLEHMQQPFYTKFITVSNHYPYTSLKGESDEEGFPLAKTND
                     ETINGYFATANYLDTALKSFFEYLKAAGVYDNSIIVMYGDHYGISNTRNPSLAELLGK
                     DPETWSEYDNAMLQRVPYMIHIPGYSKGFISNTYGGEVDNLPTLLHILGIDTSKYTQL
                     GQDLLSKDNKQMVAMRTTGQYITPKYTNYSGHLYYTDSGQEITNPDETTKAEIKAIRD
                     ATNKQLSTSDSIQTGDLLRFDENNGLKTVEVEKFNYTHSLKALKAKERKLKDRSTSIY
                     SKHNNKSTVDLFHAPSYLELQDPNKTHKTSKK"
     gene            18385..19542
                     /locus_tag="VBE22_000657"
     CDS             18385..19542
                     /locus_tag="VBE22_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEO7202131.1"
                     /translation="MNKLKVNSVVERKIKSGAQLLEKKDFDTSLVNQLVQLFSQSNQF
                     LGMAYLSPQNKGIGWLLSRQVFDFNHDYFVSLFEKSREKRQKFEKSSQTTAYRLFNQD
                     GDNFGGLTIDFYSDYALFSWYNEFVYTNRQMIVAAFKQVYPNIKGAYEKIRFKGLDFE
                     SAHLYGQEAPESFLILENNIKYSVFLNDGLMTGIFLDQHDVRKALATNLSEGKKVLNM
                     FSYTAAFSVAAAVGGALETTSVDLAKRSRELSKAHFDANQIVTDNHRFIVMDVFEYYK
                     YAKRKHLSYDVIVIDPPSFARNKKQTFSVTKDYYKLIEQALDILTPGGTIIASTNAAN
                     LTVSQFKKQLEKGFGKASHNYISLQQLPEDFTVNDKDQQSNYLKVFTIKVK"
     gene            19542..20219
                     /locus_tag="VBE22_000658"
     CDS             19542..20219
                     /locus_tag="VBE22_000658"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEO7202132.1"
                     /translation="MKIVVPVMPRSLEEAQEIDLSKFDSVDIIEWRADALPKDDIINV
                     APAIFEKFAGHEIIFTLRTTREGGNIVLSDAEYVELIQKINSIYNPDYIDFEYFSHKE
                     VFQEMLEFPNLVLSYHNFQETPENIMEIFSELTALAPRVVKIAVMPKNEQDVLDVMNY
                     TRGFKTINPDQVYATVSMSKIGRISRFAGDVTGSSWTFAYLDSSIAPGQITISEMKRV
                     KALLDAD"
     gene            20313..21380
                     /locus_tag="VBE22_000659"
     CDS             20313..21380
                     /locus_tag="VBE22_000659"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEO7202133.1"
                     /translation="MQVEVDLPNHSYHIKIEEGCFSEAGDWVSHLWQKQMITIITDSN
                     VEILYGESLVNQLKKQGFTVHVFSFAAGEASKTLEVANRIYGFLAKHHMTRSDGIIAL
                     GGGVVGDLAAFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTSFAKNMVGTFAQP
                     DGVLIDPVTLKTLGNRELVEGMGEVIKYGLIDDIKLWHILEEMDGTIDSILDSALAII
                     YHSCQVKRKHVLADQYDKGLRMHLNFGHTIGHAIEVHAGYGEIMHGEAVAIGMIQLSR
                     VAERKNLMPRGISQDIYNMCLKFGLPVHYAEWDKDVLFDILSHDKKASGQFIKIVILP
                     QLGSATVHQIPLEEMRDYLEK"
     gene            21381..22547
                     /gene="aroC"
                     /locus_tag="VBE22_000660"
     CDS             21381..22547
                     /gene="aroC"
                     /locus_tag="VBE22_000660"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEO7202134.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLKLSAKDINEDLKRRQGGYG
                     RGNRMKIETDQVIISSGVRHGKTLGSPITLTVTNKDHSKWLDIMSVEDIEERLKQKRR
                     IKHPRPGHADLVGGIKYRFDDLRNALERSSARETTMRVAIGAIAKRILKEIGIEIANH
                     IVVFGGKEITVPDKLTVQQIKVLSSQSQVAIVNPSFEQEIKDYIDSVKKAGDTIGGVI
                     ETIVGGVPVGLGSYVHWDRKLDAKIAQAVVSINAFKGVEFGLGFKSGFLKGSQVMDSI
                     SWTKDQGYIRQSNNLGGFEGGMTNGEPIIVRGVMKPIPTLYKPLMSVDIDTHEPYRAT
                     VERSDPTALPAAGVVMEAVVATVLVTEVLEKFSSDNMYELKEAVKLYRNYVNHF"
     gene            22631..22966
                     /locus_tag="VBE22_000661"
     CDS             22631..22966
                     /locus_tag="VBE22_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEO7202135.1"
                     /translation="MANVYDLANELERAVRALPEYQAVLTAKAAIENDADAQVLWQDF
                     LAAQSKVQEMMQSGQMPSQEEQDEMSKLGEKIESNDLLKVYFDHQQRLSVYMSDIEKI
                     VFAPMQDLM"
     gene            23141..24490
                     /gene="gorA"
                     /locus_tag="VBE22_000662"
     CDS             23141..24490
                     /gene="gorA"
                     /locus_tag="VBE22_000662"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEO7202136.1"
                     /translation="MSKQYDYIVIGGGSAGSGTANRAAMYGAKVLLIEGGQVGGTCVN
                     LGCVPKKIMWYGAQVSETLHKYSSGYGFEVNNLNFDFTTLKANRDAYVQRSRQSYAAN
                     FERNGVEKIDGFARFIDNHTIEVNGQQYKAPHITIATGGHPLYPDIIGSELGETSDDF
                     FGWETLPDSILIVGAGYIAAELAGVVNELGVETHLAFRKDHILRGFDDMVTSEVMAEM
                     EKSGISLHANHVPKSLKRDEGGKLIFEAENGKTLVVDRVIWAIGRGPNVDMGLENTDI
                     VLNDKGYIKTDEFENTSVDGVYAIGDVNGKIALTPVAIEAGRRLSERLFNHKDNEKLD
                     YHNVPSVIFTHPVIGTVGLSEAAAIEQFGEDNIKVYTSTFTSMYTAVTTNRQAAKMKL
                     ITLGKEEKVIGLHGVGYGIDEMIQGFSVAIKMGATKADFDDTVAIHPTGSEEFVTMR"
     gene            complement(24533..24985)
                     /locus_tag="VBE22_000663"
     CDS             complement(24533..24985)
                     /locus_tag="VBE22_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202137.1"
                     /translation="MSKGYSRQQKKSTKTNTDSTQSPAKHIKTGFSALQKTIALVGSV
                     LSIIVASITITNAINNHKDSSKTDSSTSKTVIIRESGQNSSNQVSTEKDETRASSSQT
                     LENSSASSTPSSSSNSAANNDQTSSSTNNDQTNNTTTDTSTIENTTTP"
     gene            25232..26377
                     /locus_tag="VBE22_000664"
     CDS             25232..26377
                     /locus_tag="VBE22_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7202138.1"
                     /translation="MIYFDNSATTIPYPEVLRTYQEVASKIYGNPSSLHELGTTSSRI
                     LEASRKQIASLLELKANEIFFTSGGTEADNWVIKGLAFEKQHFGNHIIVSDIEHPAVK
                     ESAKWLGEYGFEIDYAPVDDKGFVDVEALVKLIKPETILISIMAINNEIGSIQPIKAI
                     SDLLSDKPTISFHVDAVQAIGKIPTKDYLTERVDFASFSSHKFHGVRGVGFLYIKEGK
                     RISPLLTGGGQETDLRSTTENVAGIAATAKALRMVMDKEVVAIPKISKMKTIIHDELA
                     KYEDITLFSGKEDFSPNIITFGIKGVRGEVLVHAFEGHDIFISTTSACSSKAGKPAGT
                     LIAMGISTKLAQTAVRISLDDDNDMGQVEQFLTIFKQVYEKTKKVRG"
     gene            26379..27593
                     /gene="thiI"
                     /locus_tag="VBE22_000665"
     CDS             26379..27593
                     /gene="thiI"
                     /locus_tag="VBE22_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEO7202139.1"
                     /translation="MQYSEIMIRYGELSTKKKNRMRFINKLKNNMEHVLSIYPDVSVK
                     TDRDRGHVYLNGTDYHEVAESLKEIFGIQAFSPSFKVEKNVDTLVKAVQEIMTSVYKD
                     GMTFKITAKRSDHSFELDSRALNHTLGDAVFSVLPNIKAQMKQPDINLKVEIRDEAAY
                     ISYEDIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAHDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EDRNGLVIINGESLGQVASQTLESMQAINAVTATPIIRPVVTMDKLEIIDIAQKIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNTEQYEKRMDVEGLVERAVAGIMVTTIQPQAD
                     SDDVDDLIDDLL"
     gene            27695..28873
                     /locus_tag="VBE22_000666"
     CDS             27695..28873
                     /locus_tag="VBE22_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEO7202140.1"
                     /translation="MARIPYQKTLIFCVAVIIAIFILGLSKDLAQSKGQKVANNNTVK
                     TARVVANGDILLHDVLYASARQPDGTYNFTPYFKEVKSWIESADLAIGDYEGTISSEY
                     PLAGYPLFNAPNEIATTMKETGYDVVDLAHNHILDSQLAGAINTVKTFNRLGLDTIGV
                     YLKDRNKEDILIKHVNGIKIAILGYSYGYNGMEANVSKSDYEKHMSDLDTKKIKQDIK
                     KAEKEADITIVMPQMGIEYQKKPTTEQVMLYHSMIKWGADIIFGGHPHVVEPSEVIKK
                     DGQKKFIIYSMGNFISNQRLETVDDIWTERGLLMDVTIEKKGQKTVIKKVKAHPTLVE
                     AKPNGRYVEEGFPAYDYRTLVLEDYIEGGKKRSTIDQTMKDKVDKAYKEMNELVNLKW
                     "
     misc_feature    28949..29028
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            29063..29377
                     /gene="rplU"
                     /locus_tag="VBE22_000667"
     CDS             29063..29377
                     /gene="rplU"
                     /locus_tag="VBE22_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEO7202141.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLDVEAGAEVTFNEVVLVG
                     GETTKVGTPVVEGATVVGTVEKQGKQKKVVSYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            29384..29722
                     /locus_tag="VBE22_000668"
     CDS             29384..29722
                     /locus_tag="VBE22_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEO7202142.1"
                     /translation="MIKATFTRNQSGYLYSAEISGHAGSGEYGFDVICAAVSTLSINF
                     INSLEALTTCQAQLIINDVEGGYMKIDLSSIPQHKEDKVQLLFESYLLGMTNLSKDSS
                     EFVSTVVMTH"
     gene            29744..30037
                     /gene="rpmA"
                     /locus_tag="VBE22_000669"
     CDS             29744..30037
                     /gene="rpmA"
                     /locus_tag="VBE22_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEO7202143.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGANVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            30253..31158
                     /locus_tag="VBE22_000670"
     CDS             30253..31158
                     /locus_tag="VBE22_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7202144.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAAKLFVSQPSLSVAVRDLETELG
                     FQIFTRTTTGAVLTNQGMTFYENALEVVKSFDSFEKQFSQSEATEQEFSIASQHYDFL
                     PPLITAFSKCNDNFSYFRIFESTTIRILDEVAQGNSEIGIIYINSQNKKGLLQRLDKL
                     GLEFVELIPFKTHIYLGKDHPLASKTSLIMTDLEGLPTVRFTQDRDDYRYYSENFVEV
                     LDSSVTYNVTDRATLNGILERTQAYATGSGFLDSRSVNGITVIPLEDHLDNQMIYIKR
                     KDRNLSQMALKFVAVMEEYFKGFNS"
     gene            31167..31631
                     /locus_tag="VBE22_000671"
     CDS             31167..31631
                     /locus_tag="VBE22_000671"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEO7202145.1"
                     /translation="MRKIIIPIITILLIALDQLSKLWIVKHIELNQIKEFIPNIVSLT
                     YLRNYGAAFSILQNQQWLFTLITIFVVGVAIIYLMKHINGSYWLLISLTLIISGGLGN
                     FIDRLRLGYVVDMVHLDFINFAIFNVADSYLTIGIICLMIALWKEESNGNHN"
     gene            31615..32505
                     /locus_tag="VBE22_000672"
     CDS             31615..32505
                     /locus_tag="VBE22_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO7202146.1"
                     /translation="MEITIKIAGVRLDKALADNSELSRSQANEEIKKGIVLVNGQVKK
                     AKYTVQEGDRITFDIPKEEVLDYQAENIPLDIIYQDDDVAVVNKPQGMVVHPSAGHSS
                     GTLVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKNDRAHQVLAEELKDKKS
                     LRKYLAIVHGNLPNDRGVIEAPIGRSDKDRKKQAVTAKGKPAITRFHVLERFGDYTLV
                     ELSLETGRTHQIRVHMAYIGHPLAGDPVYGPRKTLGGKGQFLHAQTLGFTHPSNGENL
                     IFSVEVPEIFQTTLEKLRKN"
     gene            32681..33202
                     /gene="pyrR"
                     /locus_tag="VBE22_000673"
     CDS             32681..33202
                     /gene="pyrR"
                     /locus_tag="VBE22_000673"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEO7202147.1"
                     /translation="MKRKEIIDDVTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     LAKRIQERLKQLENLDIPVGELDTKPFRDDMKVEVDTTTMPVDITDKDIILIDDVLYT
                     GRTIRAAIDNLVSLGRPSRVSLAVLIDRGHRELPIRADYVGKNIPTSQFEEILVEVME
                     HDGYDRVSIIDPN"
     gene            33251..34321
                     /locus_tag="VBE22_000674"
     CDS             33251..34321
                     /locus_tag="VBE22_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEO7202148.1"
                     /translation="MAKKLLILEDGTVFEGLSFGSSLDVTGELVFCTGNTGYQEIITN
                     PSHNGKILVFTSPLIGNYGIHRSYSEAIIPTCLGVVVAEYSRCVSSDTSKMNLDEFLK
                     MKKVPAMSGVDTRYLMQVIKEKGFVKATLAEAGDVLSHLQDQLIATVLPTNNVEQVST
                     KTAYPSPASGRNIVVLDFGLKHSILRELSKRQCDVTVIPYNTSLEGIKNLYPEGIILS
                     NGPGNPEKLQEILNTIKELQKSVPMLGIGLGHQLIAMANGAEIMRLPVAKKGPNYPMR
                     DIATGRLETVSQFNHFTVNRLNLPHDLLVTHEGLNDQEIVALRHRSFPVMSVQFYPEA
                     APGPHDVTYFFDEFLEMIGSRA"
     gene            34377..>35855
                     /locus_tag="VBE22_000675"
     CDS             34377..>35855
                     /locus_tag="VBE22_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEO7202149.1"
                     /translation="MLNTKDKQPILLIIFGQKMKEYDDLIRSTVERLSKRDRPVSILI
                     SKELNHVIHHYDAQFENLETLVGYINTHEDSLIYPLCLDKNHELFQEFLAWQSHYSRQ
                     YISDNLSEKSVQAIDNNQDFVQVDCLVMRDSLNNCLYVSDLEYIESNKTTGKSLAIVP
                     SQTLSDAARQTIRDVAFDVCRKANIIGVCYFSFLIDLNSLDYHIISLSSGLSHQSILF
                     ETITTYPVLEIATKLTVGYTFSQLKHSYYPNTSAFLEPQLDYVATVSFSFEKVDYIFF
                     ARNIEQLFLNLLEASSHDHFPFLSDISEEDLMFALIQKKENRLAYLLEAFRRGFDLYD
                     LSSVTKINPFYLDKCLHIVELYENLNKSQYNVDIYKEAKRYGFSDDYIASSWQISLID
                     MLEYRKKHSVAPVLKQVEQSSGVLTGHQIQYFRSYDWHSDYISSGCQKALIMVDKGYS
                     LVKLNELIKQIKQTHLELLIVTNQPLLIEQLNDTSIIFDTIGI"
BASE COUNT        11714 a         5545 c         7035 g        11561 t
ORIGIN      
        1 ttaatcgcta tcagctaaat tcaaataatt tttatttagg agtaattttc catggaagac
       61 cctggcagtc agagtttgtt actacaattt gttgttttat taatattaac cctatttaac
      121 gccttctttt cagcatctga aatggcttta gtatcgctaa accgctctaa agtagaacag
      181 aaagctgaag aaggggacaa aaggtatcga cgtcttttgg acgttcttga aaatcccaat
      241 aattttttat caacaattca agttggtatt acttttatta gccttttgca aggggctagt
      301 ttatccgcct cattaggaca tgtgatttca gggtggctag gaaattcagc aacagcccgt
      361 acagcaggga gtattattgc gctaatcttt ttaacatatg tctctattgt tctaggagaa
      421 ttatatccta agcgtattgc tatgaatttg aaagatcgtc tagcaattgt ttcagctccc
      481 attattatct ttctggggaa aatcgtaagc ccctttgtat ggttgctctc agcttcaact
      541 aacttattga gtagaatcac tccgatgact tttgatgatg ctgacgagaa gatgacacgt
      601 gatgaaattg aatatatgtt gacgaatagt gaagaaactt tggatgctga agaaattgag
      661 atgctgcaag ggattttctc gctagatgaa atgatggcgc gtgaagttat ggttccgcgc
      721 actgatgctt tcatgattga catcaacaat gatgcacaat caaatattga aggaatttta
      781 tcacaaaact tttctcgtgt ccctgtttat gatgacgata aagatagagt tgttggtgtt
      841 ttacatacca agcgcttatt ggaagtgggc tttaaaactg gttttgatac tatcgacttg
      901 cgaaaaatac ttcaggaacc tctttttgtt ccagaaacga tttttgtaga tgacctttta
      961 aaagcattac gcaacactca aaatcaaatg gccattttac tagatgaata tggtggcgtt
     1021 gctggtttag taactctaga ggatttattg gaagaaattg tcggcgaaat tgatgatgaa
     1081 acagatacag ctgaacaatt tgtcagggaa atcgatgaga atatctatat tgttctagga
     1141 actatgacgc ttaatgaatt taatgattat tttgagacag agttagaaag tgatgatgtt
     1201 gatacaattg cgggttatta tttaactggt gtgggtagta ttccaaacca agaggaaaaa
     1261 gtagcttacg aagtagactg caaagataaa cacatcactt tgattaatga taaagttaaa
     1321 gatggtcgta taacaaaatt gaaggtgcta ttgtctgata tagaacagaa tattgaaaaa
     1381 gactaagatt acttagtctt tttttcatga aaatgatata ataaagtcat aaaaacggtt
     1441 acaaggtgat gaaatggcag aaatagacta taaaaaagta acaggaatga tccattctac
     1501 agaaagtttc ggctctgtgg atgggccagg tattcgtttt atcattttta tgcaaggatg
     1561 taaaatgcgt tgtcaatact gtcataatcc tgatacttgg gaaatggaaa ctaacaattc
     1621 aaaggaacgt accgttgagg atgttctaaa ggaagctctc cgttataaac atttttgggg
     1681 aaaagatgga ggtatcacag tttcaggtgg tgaagcaatg ctgcaaattg cctttattac
     1741 agccctcttt attgaggcta agaaactagg tattcatacg acgctagata cttgtggttt
     1801 tgcttatcga gcaacaccag aataccatgc tatccttgaa aaattgttag atgttactga
     1861 tttggtactc cttgacttaa aagaaattga tccagaacaa cataaaattg taactcgtca
     1921 gtctaacaaa aatattttac agtttgctag atatttatct gatagaggta ctccggtatg
     1981 gattcgtcat gttttagttc ctggattgac tgatattgat gaccatttaa aacgtttggg
     2041 tgaatttgtc caaacacttg ataatgtcga taaatttgaa gtattgcctt atcatactat
     2101 gggagagttc aaatggcgtg aattaggaat tccgtatcca ttagctggtg tgaaaccacc
     2161 aactccagaa cgtgttaaaa acgctaaaga tatcatgaaa acagaatcct atacagaata
     2221 tctaaaaagg attcaaaatt agttttaagt tgaattttat tcaacttttt tagttgcaaa
     2281 gggcttccta gtaagatttt ttgctaagtt attcaatttt tgctacaata aggtgaatat
     2341 aaaagaagag gtaaaaacat gtctaaaatt ttagtctttg gtcaccaaaa tccagattca
     2401 gatgctattg gatcatcagt tgcttttgca tatctagcta aagaagcttg ggggcttgac
     2461 acagaggccg ttgctttagg aactccaaat gaagaaacag catatgtttt agattatttt
     2521 ggtgttcaag cacctcgagt agtagaatct gcaaaagcag agggtgtaga gacagtcatt
     2581 ttaactgacc ataacgaatt ccaacaatct atttctgata ttaaagatgt gacagtttat
     2641 ggtgttgttg atcatcatcg tgttgctaac tttgagactg ctaatccgtt atatatgcgt
     2701 ttggaaccag ttggctcagc atcatcgatt gtttaccgta tgtttaagga aaatggagta
     2761 tcagtaccca aagagttggc tggtttatta ttatcaggtt tgatttcaga tactcttctt
     2821 ttgaaatcac caacaactca tgcttcagat attccagttg caaaagaact agcagagatt
     2881 gcaggtgtaa acttagaaga atatggttta gaaatgctta aagcaggtac taacctctca
     2941 agtaagactg cagcggagtt gattgatatt gatgctaaaa catttgaatt aaatggagaa
     3001 gctgttcgtg ttgcacaagt taatactgtg gacattaacg atattttagc acgtcaagaa
     3061 gagattgaag ttgctattca agaagcaatt gtcacagaag gttattctga ttttgtattg
     3121 atgattactg acattgttaa ttcaaattca gagattttag ctctcggttc aaatatggct
     3181 aaagttgaag cagcttttga atttacactt gaaaataacc acgcttttct tgctggggca
     3241 gtatcacgta aaaaacaagt tgttcctcaa ttgacagaaa gctacaatgc ttaattaatc
     3301 atgtaaaacg ggctacgtcc cgttttttta gtcatacatt taggagcgct tttatgatat
     3361 taacgtttaa tccaggaaag ttagaaagac aagagttttt taaggagctt attaactatc
     3421 tttggataca tgatgatgta acactaagac aaataaagtc ccactttaca gactactcta
     3481 aaattgaccg tttgttagaa gaatatatta accatggata tatccttcgc caaaataaac
     3541 gctacagcct taatcttcct tttctgagtt cgctagatgg attagtatta gatgatttgg
     3601 ttttcataga ttctgatagt caaatctatc agttattaca gaagcgaaaa tttgtgacaa
     3661 atttagataa ccaaaccaat cacttggtat ttgtagaaga gacagatttt gaaagaaaca
     3721 ctttgactct ttctaattat ttctataaac taactaacgg ttatccactt tcaagagaac
     3781 aaaaaaagct ttatcaactt ttaggagatg ttaattcgga atatgcttta aaatatatgt
     3841 caagctttat attgaaattt cttcgcaagg atagtgttaa gcaaaaacgt acagatattt
     3901 ttatccaagc tttagaatta cttggatata ttagcttaaa ccaagatact acctatcgtt
     3961 taaatgctaa acttgatgtt gaagctttaa agatttactt aacataaaaa aagactggaa
     4021 gtttgtccag tcttttttag tcaacttttg tcatcaacca gagaaaatag ggggcaccaa
     4081 ttattgatac aaccaatcca gtgggtatac ctgtaccaac taagaggaga cgacctatag
     4141 tatctgatac caataggata atagcaccaa taagaagaca gctaggtagt ataattttat
     4201 ggttatttcc taatagtcgt ctacttaagt gcccagcgag taagcctata aaagtaatat
     4261 taccaattaa gacgacgcta atagcagcaa ggctacttgc cagcatcaaa gtatatattc
     4321 tttccttttt taaatgaagt cctagagcga gagcagtctg ctcgtttagg ttcataatat
     4381 taaggtggcg actgcgacta tatgttaatc cccataacaa aatgagaatc gggataaaag
     4441 tgataatggt agtccaggaa ctaattgtaa tttttccact aagccattca acgatcatat
     4501 ccattttttg actattagac tgactaataa tagaaatcat aatgcctgaa atcattgtag
     4561 atattccgat accagtgacg attaatcgtg tagggctaat attgtgattt cttctgtatg
     4621 acataagata gacaagaaaa atagttacca accctccaaa catagcaaat aggggtaaaa
     4681 agtataatat agttggattg ctaacattaa atgttcctat ggttagagcg attactaatc
     4741 cagctccagc attgatacca agaataccgg aatcagctag tggatttttg gttaacgtct
     4801 gtaatagcaa cccagcaatt cctaaagaag ctcccccaaa gatacagaca ataatacgag
     4861 gtaaacgaat gttagtaatg ataaaaagga aggcatcatc agattttcca cttattagtt
     4921 ttaaaacgtt aataacggaa gtattagcat agccaagtga aattgatact agtattaata
     4981 atagtaggat aatgacagat gatattaaaa caaaaggagc tttatttttt tgaatcatag
     5041 atttttccct tttctaatta accataggaa acatggaagc cctacgatac tgattataga
     5101 actaattggt gtctctgaag ggggatttat aatacgagat gataagtcta cccagatcat
     5161 gaaagtagcc cctgaaaatc ctattaaagg taataacaga cgataatctt tgggaataaa
     5221 aagcttaata aaatgtggaa taatcaatcc aatgaaagaa acggttccta tcaacgcgac
     5281 tgatgaagcc gataaaaaca aaacaataag taatagaaac gcagtcatta attgagtttt
     5341 ttgtcccaat gctttggcca cagattcatt aaggctcaag atagttaatt gatgtgctag
     5401 gagttgtgaa ataagtaaac ctaaaataat gataggtgct attatgatta gcattttcca
     5461 gttaatttgg gataaaccac ctgcttgcca accgataaca gttcgattga gtttaaagta
     5521 taatgtaacc acttgtccaa cagacgtaaa aagtgtggag atcatcgctc cagctaaaat
     5581 gagtcgtagt tggtggtaac ccttttgctt tgtataagag agtgtgaaga ctaaaagaca
     5641 agaaatgaca gaccctagta agcagacaat gagaatcgta ctatagtgca tgctccctaa
     5701 aaaagcatat gcaacaacaa gggctaaccc agttccggcg ttaataccta gtagtccagg
     5761 atcagcaatg gcgtttcgag tgactccctg cataatagca cctgcttgtg acatagctgc
     5821 acctactaag atagctgcaa ttatgcgtgg tagtctaatg tcaaaaataa catcctgagc
     5881 attagagttt tttaagggat tttttaaaac agttattaaa tcactagtct tgaaattgat
     5941 agcaccaaat cgtaagctgg cgtaacaacc aataagaaat agtataagtg ttatgaaaaa
     6001 aacaagccaa aaatgatttg acttattttt ttgaacgagt ttttttgaag ctttaaacat
     6061 aagaggagct ttcttttaat ttgtattttc tttgatagcc tttgtaaatg attttaattg
     6121 agcttctaaa gatagagggt cagagaaata aaacacgtcg tagttacttt ctatgatgtg
     6181 cccttttttg acagctggta aattcttcca gacatcactt tctttaagtg atgaagctgc
     6241 ttttttagtc gttttgttta tattaacaag ggcataatct ccaacgtaat caccgattgc
     6301 ttcttgcgaa acggtaaacc accctttttt aaagacatct tttttgactt tttctggggc
     6361 agcataacct agtgaatcat agattagttc tccaccgcgt ccaaaattat taccatataa
     6421 atagatattt ttatcataaa aatccataat agtaaaagta gtgttaggct ttaagatatg
     6481 gtgtaaatct tttttggcag cgagagtttt agttttccat tggctaaccc actgattagc
     6541 ttctttttct ttaccgaata ctttccccaa ggctggcatc atatctaaat aattttgtgc
     6601 accatattta ataactaaag ttggtgcaat ttttttcaga gtattgatgt ttggatcttg
     6661 atcgaaaacc atgattaaat caggtttttg tgcggcaata gcttctgtat catcagcagt
     6721 taatttttta gcttctttca gttgcttacc aaaaacgggg ctatcttttt ctaagtctaa
     6781 actgtaactt gaaacattaa cacctagttt taataaatac ccagtgtaag aatatgtaaa
     6841 attaattact tttttcggat tttcaggaat ttttccataa taggtgaagc cttcaatttt
     6901 aggcatttta gaaatagttg ttttagtgct ttcttgttta gtttgttgtc cgcaagatac
     6961 caaaaagaag gtcagtagtg tgaggacaat aattccaatt tttttcataa gataacatct
     7021 cctaaataat attaattttg atcagttgat aattgataag ataacaaaac aggttgttta
     7081 ctaatagggt cctgaatgat atgagcatca atcttaaaaa tatctttgat aatatcttta
     7141 gtcatgatct gactaggact accttcatag ataattttac cggttttcat tgcaactaga
     7201 taatcagaat agcgcgcaga caggtttagg tcgtgtagga ccataataat tgttttttgc
     7261 gtttcgttat taagtttttt gagcaattcc aagacttcta attgatgatt catgtcaaga
     7321 taagttgtag gttcgtctaa aaagatagta tctgtatctt gtgctaaagc catagcaatc
     7381 catacttttt gcctctgacc tcctgaaagg tcatcaacat ctctattagc aaattgcgct
     7441 acgttagtca tttccatcgc ccaatggatt ttactgcgat cgtcatttgt caaattgcca
     7501 aggtattttt ggtgtgggaa ccgtccataa gatattagtt cataaactgt tatcccttca
     7561 gttgcttcta acacctgtgg gagaagtgca atcttcttag caatttcctt tgtgggtaaa
     7621 tgtgcaatat catgaccatc aatagtgata gtaccatctt ttattttatg gatgcgtgtg
     7681 agagccttca aaagagtaga ctttccacat ccattggcac caataatcgt tgttattttt
     7741 tggttaagta tactgaggga aagattattg atgatttctt tctgatcgta agatacgata
     7801 atgttttctg cttttatgtg agacatagag aacctttcta gcaaaaaatt aagttcatta
     7861 taacatagaa aaaaggctta caaaagcctt ttttagcatt aattaagaaa aatgtgaaca
     7921 aaatgataat tatttagaat gattcaaaat agtatcaata atcattattg tttagctttt
     7981 ttatcgttta aggtattttt tcgcaacttc taaatctcca gagtaattgt cacgatttcc
     8041 tttgataatt tgataagcat ctgccccttt accagcgata ataattgcat ctagtttgca
     8101 attagtaaga gtactggcat tagcaattgc ttcttctcga tcaatgatga tagtaactgg
     8161 acgattaata tggcttgcta tttcttgtga gataactaag ggatcttcaa aattaggatc
     8221 atctgcagtg agtatcactt gtagattagg atgttgatta atgacatctc cgaagtcttt
     8281 acgacggctt tgacctttat taccaggagc tccgagcact aatataatat cccctttttg
     8341 gtgttcctca acaacagcta ataatttctt caaactgtct ccgttgtggg catagtcaac
     8401 gaaaatcttt gcgccgttgg tttgtgttag gacttccatg cggccaggga cggtagtttg
     8461 tgcaattccg ttttttatat cttcaattga aacaccaagt cggagacaag caagtccagc
     8521 tgcgatagca ttttcttgat tgaattttcc aatcagcttg atatcgtagg tgttctccaa
     8581 ctttcctttg acatggaaag aaaaggcctg tgattctgtg ataacatttt cagaataatc
     8641 tccatagaaa tcatgaggaa tatattctac ctgttctttg acaatattaa aatgatccat
     8701 ctgactattt acaacaacag cattgctatt ctccatcaag agccgtttgt ggaaaaaata
     8761 atcttcgaat gtagggtgct cgataggacc aatatgatcg ggactaatat ttaagaaaac
     8821 gccaacgtca aaggttaaac cataaacacg tttggttagg tatgcttggc ttgatacttc
     8881 cataactaag tgtgtcattt gattctcaac agctgtagcc atcatccgaa ataaatcaag
     8941 actctcgggg gttgtgagat gtgatttaaa gaaagattta ccatctaatg ttgtgttcat
     9001 agttgagagc atagcaggac gatggtttac ttttaacata tgataagcaa agtaagccgc
     9061 agttgttttt ccttttgttc cagtaaaagc tagaagttta agtttgtttt gtgggttatt
     9121 atagaaagac atagatatca aactcatggc ttttttgata tcattgacta atatcactgg
     9181 aatatcggcg ccgtaatcta tctcagaaac gtaaaaagaa aggcctgctg tgattgcaga
     9241 atctagatat tcttttttaa aagtagcacc cttagcgaaa aataaagtat cagatgaaat
     9301 ttgacgacta tcatatgata aggcattaaa agttacattt tgtgtccagt tataataata
     9361 atgctcatga aacaaaatct ctctgaaatt atggtcattt tttaaaattt ctaaaatttt
     9421 atctattgta atcatactcc ttattttaga ctttatgttc tcgttttaca agtctaaggt
     9481 aaaagtttat aataaagtga tatttctgag atttttgtct tttaaatgct aaataatatt
     9541 gaaaatagaa agtttaccat gtcacaaaaa acaacaaaag ttagtcagca agaacaaatg
     9601 gtcaaaggga cggcttggtt aacagcaggg aatttcatta gtcggttgct gggagccatc
     9661 tatattatcc cttggtatgc ttggatggga aagcatgctg ccgaagcaaa tgctttattt
     9721 gggatgggat atgaaatcta tgctttattt cttttgatat caactgttgg aattccagta
     9781 gcagttgcaa aacaggtatc taagtataac actttaggta aagaagagat gagcatttac
     9841 ctggttcgga aaatcttgca gtttatgctg attttaggtg gagtttttgc attaattatg
     9901 tatattggtt cacctctatt tgcttcactt agtaaaggag ggcaagaact cgttccaata
     9961 ttgcgtagtc taactctagc tgttttagtt tttccttcaa tgagtgtttt acgtggtttt
    10021 tttcaaggtt ttaataattt aaaaccgtat gccattagtc aagtcgctga gcagattata
    10081 cgtgtcatat ggatgctatt aactgctttc tatattatgc gtctaggatc aggagattat
    10141 attgccgctg tgacacagtc aacattcgcc gcctttgtcg gtatgtttgc tagtatagct
    10201 gtgctgcttt atttcttatg gcgttataat atgctatctg ctttgattgg taaaactcca
    10261 gagcacatta aattagatac taaagaaata ctaattgaga ctgttaagga agcaatccct
    10321 tttattatta ctggtgcagc aattcaaatt ttcaaattga ttgatcagtt ttcatttggg
    10381 aatacgatgg cgctctttac gaattattca agtgaagaat tacgggttat gtttgcttac
    10441 ttttcaagca accctggtaa agttacgatg attctaattg ctgttgctac cgcaattgct
    10501 ggagttggta ttccattgtt aacagagaat tttgttaaga atgataaaaa agccgccgct
    10561 cgtttggttg ttaataattt acaaatgtta ctaatgtttt tactgccagc tgttgcagga
    10621 tccgttattt tagcaaaacc tttgtataca gtcttttatg ggttaccgca gggacaagct
    10681 ttagggcttt ttgtcatatc tcttattcaa acaattattt tatctattta tactgtttta
    10741 gcaccaatgc tacaagcatt gtttgaaaat cgtaaagcta ttatttactt cctatatggt
    10801 ctggtggcaa aagttatttt acaagttccc tccattttcc ttttccatgc ctatggacca
    10861 cttttctcaa cgacagtagc tctttgtatc ccagtaattc ttatgtattt gaaaatacat
    10921 gaaattacag gatttaaacg ccaagctatt cgtcgtacaa gtgctttagt ccttattcta
    10981 acgctattga tgagctttat tatttctatg ataatatggt taatgaacct ggttattgta
    11041 cccgatagta gactggtaag tctagtttac attatcgtta tcggtgcaat aggtttaggg
    11101 gtatatggct ttatggcact ggccacccat ctacttgata agatgattgg cagtcgtgct
    11161 caagacttac gccgaaagtt gcaccttaac taatgaaaaa actaggttag acagagttgt
    11221 ttaacctagt tttttgacga ccaaaattat ttgtgaaatt ttaaggtagt cacaatatag
    11281 tgagcttatt tttatgttat aattaaatgg agaaatatga ggtgaaagat gtcttacgaa
    11341 aagcttttag aacgattttt aacatacgtc aaaataaata caagaagtaa tcctaatagt
    11401 acacaaacgc caacaactca aagtcaagtt gactttgctt taacagtttt aaaaccagaa
    11461 atggaagcaa ttggtttaaa agatgttcat tatttacctt ctaatgggta tttggttgga
    11521 accttacctg ctacaagcga ccgcttatgc cataaaatag gttttatatc ccatatggat
    11581 acagctgatt tcaatgctga aaatattact ccacaaattg ttgactataa aggtggagat
    11641 attgaacttg gagactcagg ttacatttta agtccaaaag attttccaaa tttaaataat
    11701 taccatgggc aaacattgat tacaacagat ggtaaaacct tactgggagc agacgataag
    11761 tctggtatag cagaaatcat gacagctatg gaatatttgg cttcgcatcc agaaattgag
    11821 cactgtgaaa ttagagttgg ctttggacca gacgaagaaa ttggtatagg tgcagataaa
    11881 tttgatgtta aagattttga tgttgatttt gcctatacag tggatggtgg accactagga
    11941 gaattacagt atgaaacctt tagtgcagct ggtttggagc ttacatttga aggacgaaac
    12001 gttcaccctg gaactgcaaa aaatcaaatg attaatgctt tacagcttgc tatggatttt
    12061 catagtcaat taccagaaaa tgaacgtcct gaacaaacag atggctatca aggattttat
    12121 cacttatatg atttaagtgg aacagttgat caagctaaaa gttcatatat cattcgagat
    12181 tttgaggaag ttaatttctt aaaacgtaag cacttggctc aagatatcgc tgataatatg
    12241 aatgaagcat tacaatctga acgtgtaaag gttaaactat acgatcaata ttacaacatg
    12301 aagaaagtta ttgaaaaaga catgacacct atcaacattg ctaaagaagt aatggaagag
    12361 ttagacatca agccaatcat agaaccgatt cgtggtggta cagatggctc taaaatttcc
    12421 tttatgggaa tccctactcc taatcttttt gcaggtggtg aaaacatgca tggacgtttt
    12481 gaattcgtta gtctacaaac aatggaaaaa gcagttgatg ttattttagg catcgttgct
    12541 aaggattagt aaagaaatat cagattggag tggttaaatg ataaaattat ttgggaagat
    12601 acgttatcac tggcaacctg aactttcttg ggctattatt tattggtcaa tagccattgc
    12661 accaattttt attggtttat ctctgctata tgaaagaact gagattccga gtcaggtatt
    12721 tgttctattt gccatcttta ttgttttagt aggcattgga tttcatcgct attttgttat
    12781 tgaagaagat ggatatctta ggattgtatc ttttaacttt ttaagacgat ctaaatttcc
    12841 aattgaagat attgctaaaa ttgaagtcac taaatcaagt gtcactatca aatttaataa
    12901 taaccatgag cgtatttttt acatgcgaaa atggcctaaa aaatattttt tagatgcact
    12961 agctattgaa cctaaattta agggagaagt ggagcttctc gataatttga ttaaaatgga
    13021 ttattttgaa tgttatcgct acgataaaaa agctcttaca aaatcgtaag agctttggtg
    13081 ggacatgatt taactgctaa aacagtatct tgatcagaat ttggaacctc tttttctagg
    13141 ttatctgtat cggaaaattt aacgatacca tcatcttgat aatcaaaaat atttgaatat
    13201 gtttggcaaa gcccgcaggc aatacatttt tctggaatta gtgcaacttt catagtatta
    13261 ttgtaatact tattgatacg attaacaagg gggaagtcat ggcgaaaaaa ccatgggaaa
    13321 aaaaagttgt ggaaaacaat agtcatagaa aagacaaaat tacaagaacg agtagaggtg
    13381 tggtgtcaag tactccttgg cttaccgctt tcttgagtgc attctttgtt attgtcgttg
    13441 ctattttatt tatagtattt tatacttcaa atagaggaga agacagagca aaagaaactt
    13501 ctggttttta tggtgcttca agtcaaaaag ttaatagttc taaaacgaaa aaagcatcta
    13561 caagtaagaa aacaacgtca tcatcaaaaa ctaaaactag tgataaatca acaacatctt
    13621 ctagtacagt agcaagttcg agtgagacat cgacatcatc ttcagtaaac ggtggaacaa
    13681 tcgtagttca accaggtgag ggagcagcct ccatagctgc tcgtgcaggt atctcagttg
    13741 ctcgtttaca agaacttaat cctggtcata tgacctcagg gtattggtat gctaatccag
    13801 gcgatcctgt ggttgtaaat taaggggagg attatgaact caattaatat tgctattgat
    13861 ggaccagcat caagtgggaa aagtactgtc gctaagatta ttgctaaaaa tttgaattac
    13921 acttaccttg atacaggtgc tatgtatcgt tgtgctactt atctggcttt acagcatgga
    13981 tatgaagcac aagatgtttc gaaaatttta ggactcctag ctgaaagacc gatttctttt
    14041 ggtaaagcag aagatggcag ccagacagtc tttattggca cagaagaagt tactttagct
    14101 atccgtcaaa acgatgttac taacaatgtc tcgtgggttt ctgctatccc agagattcga
    14161 gaagagttag taaaccagca gcgtcgtata gccaaagatg gtgctattat aatggatggt
    14221 cgtgacatag gaacagttgt tttgccagat gcagaattaa agattttttt agttgcttct
    14281 gttgatgaaa gagctgagcg tcgctttaaa gaaaatcaag aaaaaggcat tgagagtgat
    14341 tttgaaacac tgaaatcaga aattgctgct cgtgattata aagatagcca tcgagaagtt
    14401 tcaccattaa aggcagcaga agatgctatt gaatttgata ctacaggagt atcaattgaa
    14461 ggtgttgtaa cttttattca agaaaaagca gaaaaaataa ttgacatgaa gaattagtca
    14521 tgatatacta gttgcaatga gaaaagcaga agtgagaact tctcgccttg cgacttaggt
    14581 tgtctggccc aacatgtcaa gattccatct aggaagatta tatgtgcggg cttgatttat
    14641 caagtccgct ttcgtttttc tctaaaaaaa taaagaggtg aagatcatag ctaaaaaaga
    14701 tctattcatt aacgatgaaa ttcgcgttcg tgaagttcgt ctggttggtc tcgagggtga
    14761 acaattaggt attaaaccat tgtctgaagc tcaagcaatt gcagatgatg ctaatgttga
    14821 tttggtttta attcagccac aagctacacc acctgttgcg aaaattatgg actatggtaa
    14881 gttcaaattt gagtatcaaa agaaacaaaa agaacaacgt aaaaaacaaa gcgttgttac
    14941 tgttaaggaa gttcgtctta gcccagttat tgataaaggg gattttgaaa caaaacttcg
    15001 taacggtcgt aagttccttg aaaaaggtaa caaagttaaa gtttctatcc gattcaaagg
    15061 acgtatgatt actcataaag aaattggagc aaaggtattg gcggaatttg ctgaagcgac
    15121 acaagatatc gctattattg agcaaagagc taagatggat ggtcgccaaa tgtttatgca
    15181 gcttgcacca attccagaca agaaataatt gtcgacttta tagtattatt taagaggaga
    15241 atttaaaatg ccaaaacaaa aaacacaccg cgcatcagct aaacgcttta aacgcacagg
    15301 ttcaggtgga ttgaaacgct tccgcgcgtt tacttcacac cgtttccacg gtaaaactaa
    15361 aaaacaacgt cgtcatcttc gtaaagctac tatggtttca tcaggagatt tcaaacgtat
    15421 taaagcaatg cttactcgcc ttaaataata aggtctctgt aaacatttac tacatattat
    15481 aaaggattat ttggaggaaa tataaatggc tcgtgttaaa ggtggagttg tttcacgtaa
    15541 acgtcgtaaa cgcgtactta aattagctaa aggttactat ggtgcaaaac atatcttgtt
    15601 ccgtactgca aaagaacaag taatgaattc ttactactat gcataccgtg accgtcgtca
    15661 gaaaaaacgt gacttccgta aattatggat cactcgtatc aatgcggctg ctcgtatgaa
    15721 tggtttatca tattcgcaat tgatgcacgg tttgaaatta gctgaaatcg aagttaaccg
    15781 taaaatgctt gctgatttag cagttaacga tgcagcagct ttcacagctc ttgcagatgc
    15841 agctaaagct aaacttggta aataagaatt aaaaaagttg ggtctaccta acttttttaa
    15901 ttactagaaa gaaagtacct atattataat ttaagtgaaa agtttcgttc tatatatgta
    15961 gaatgaaact tttttgtttt aattcgatat ctttattatt gttataattt tcctttttga
    16021 tttttattgt tttatagtat tattccgtat aaaaagcacc tcaatcgttg gattgaggtg
    16081 tggattaaac tttaaatggc aaggattatt ttttagatgt tttatgtgtc ttattaggat
    16141 cttgcaattc caagtaactt ggagcgtgga agagatcaac tgttgatttg ttgttatgtt
    16201 tgctgtatat gcttgttgaa cgatctttca atttacgttc tttagctttg agtgctttaa
    16261 gagaatgtgt gtaattgaat ttttcaactt ctacagtttt caaaccattg ttttcatcaa
    16321 atcgtagtaa atctccagtt tgaatcgaat cacttgtaga taattgttta ttagtagcat
    16381 ctcgaatagc ttttatttca gctttagttg tttcatcagg atttgtgatt tcttgtcctg
    16441 aatcagtgta atagagatgt ccactatagt tagtgtattt aggagtaata tactgaccag
    16501 ttgttctcat agcgaccatt tgtttattat ctttagataa taagtcttgt cctaactgag
    16561 tatatttaga cgtgtctatc cctagaatat gaagtaaagt tggtaaatta tctacctcac
    16621 caccgtatgt attactgata aaaccttttg aatagccagg aatgtggatc atatatggaa
    16681 cacgttgtaa catagcgttg tcgtattcag accatgtttc agggtcttta cctaataatt
    16741 cagctaagct aggattgcgt gtattagaaa taccgtagtg atccccatac attacaataa
    16801 tagaattatc atacacacca gcagctttta gatattcaaa aaatgattta agagctgtat
    16861 caaggtagtt tgctgtggcg aaataaccgt taatcgtttc atcgttagtc tttgcaagtg
    16921 ggaatccctc ttcgtcagac tctcccttca aacttgtgta agggtaatgg ttacttactg
    16981 taataaattt agtataaaac ggttgttgca tgtgctctag atatttgata gagtcagcaa
    17041 acatatattt atcattcaaa ccgtattgga atgaattatc tttggtttgt tttgagaagt
    17101 agctagagtc aaagaagtag tcataacccc actgtttgta agcattattt ctattccaaa
    17161 atgttccgac gtttccgtgg aagaccgcac tactgtaacc tccattttga gctaagatat
    17221 gtggagcagc aaattgtgta ttttcaccac cataattaac catgaatgaa ccactactta
    17281 gaccaaaaag tgagttttcc atgagtgtct cagcatcgga tgtcttacca gctttgactt
    17341 gatggaagaa gttagagaag gaaatagtct ctttcgaatg ataaaggctg ttaataaatg
    17401 gagtaaccac atgttcttta ccatcaacat ttaatttata gtcaatcaag aattgttgaa
    17461 aactctccaa gtgaatcatg ataacgtttc gcccttttcc gataccataa tattctggat
    17521 tgggtttggc ataatgctct gctacatatt ttttggcagt cgccaattct tgtgcagtgg
    17581 caccatttct ttctttctgt gcttgataag tttgattacc actataaatc gaaaatgatg
    17641 gcaagccaag agctttaaca atataggtgt ttgaaaaacc acgacttagt agttctggac
    17701 gatcaatttc agctaagaat aagttaattg aaaagagtaa accagacaaa gctgtgatag
    17761 aaaaactagc acgtttatta aatggacgat catctaagtg gattttcttg gttgcaaaca
    17821 agaaaataag gataatgaaa tcgaaaacgt agacaagatc ccaaattctc aatagattaa
    17881 gagcagagtc tccaaggcca gccgaagttt tactactagc caagacggcg ctaactgtaa
    17941 tgaaatcgga aaactctcta tagtagattg cgtttgcaat tagaaggata tttacgatag
    18001 cataggtgat aaaagcggta atataaaaag ctttggttcg cttaacatat aaggccaaac
    18061 ctattattaa gagtcctaag gggatggggt ttataatggt caaaaacaac tgataggaat
    18121 tttctaaacc taaattaaaa tcagtatggt aagcccaaat tgtctttaac caatatataa
    18181 ctactaacaa taaaataaag ccgagtcgtg tattgatcgc acggctagca aaatctttaa
    18241 tttttttcac agtaggaact tcctttaatt tttttcctaa agataaaatc tcagattaaa
    18301 ttataacata aaattttgta acatacggta aagaaaggtc gaattcagta tgaaaaaaga
    18361 taaaaatttg ttataatact gtttatgaat aaacttaaag tgaattctgt cgttgaaaga
    18421 aaaatcaaat caggtgctca gttactggaa aaaaaagatt ttgataccag tttagttaac
    18481 cagttggttc aacttttttc acagtcaaat caattcttag ggatggccta tctttcacca
    18541 caaaataaag gtattggttg gttactatca agacaagttt ttgattttaa ccatgattac
    18601 tttgtatcgc tattcgaaaa atctagagag aagcgtcaaa aatttgaaaa atctagccaa
    18661 acaacagcct accgcttgtt taatcaggat ggagataact ttggtggcct aactatagat
    18721 ttttatagtg actatgctct tttttcgtgg tacaatgaat ttgtttatac taatcgacaa
    18781 atgattgttg cagcctttaa gcaggtctat cctaatatta aaggggcata tgaaaaaatt
    18841 cgtttcaaag gtttagactt tgaaagtgct catttgtacg gtcaagaggc tcctgaatca
    18901 tttttgattt tagaaaataa tatcaaatat agtgtctttt tgaatgatgg attaatgaca
    18961 ggtattttcc ttgaccaaca tgatgtcaga aaagccttag caactaatct atcagaaggt
    19021 aaaaaagttc taaatatgtt ttcatacact gcggcttttt ctgtagctgc agctgttgga
    19081 ggggcattag agacaacttc tgttgattta gcaaaacgct cgcgtgaact ttcaaaagca
    19141 cactttgatg ctaatcagat tgtcacagat aaccatcgat ttatcgttat ggatgttttt
    19201 gaatattata agtatgccaa aagaaaacat ctatcatatg atgtgattgt tattgatcca
    19261 ccaagttttg ctcgaaataa aaaacaaact ttttcggtta ctaaagatta ctataaatta
    19321 attgaacaag ctttagatat tttaacccct ggtggaacta tcattgcatc aactaacgca
    19381 gctaatctaa ccgtatcaca atttaagaaa caattggaaa aggggtttgg taaagcttca
    19441 cataattaca ttagcttaca gcagttgcct gaagatttca ctgtaaatga taaggaccaa
    19501 caaagtaatt atttaaaagt atttacaata aaggtaaaat aatgaaaata gtagtaccag
    19561 taatgcctcg cagtcttgaa gaggctcaag aaatagattt atcaaaattt gatagtgttg
    19621 atattattga atggcgagct gatgccttac caaaggatga cattattaat gtagctccag
    19681 ctatttttga gaaattcgca ggtcatgaaa ttatttttac tcttcgtaca acgcgtgaag
    19741 gtggtaatat tgtcttatct gatgctgagt atgttgagtt aatccagaaa attaattcta
    19801 tctacaatcc agattatatt gattttgagt atttttcaca taaagaagtt tttcaagaaa
    19861 tgctagaatt tccaaattta gtcctgtctt atcacaattt tcaagagaca ccggagaata
    19921 ttatggagat attttcagaa ttaacagccc tagcaccacg agttgtgaaa atcgcagtaa
    19981 tgccaaagaa tgaacaagat gtcttagacg ttatgaatta cactcgcggt ttcaagacta
    20041 ttaatcctga tcaagtttat gcgacggtat ctatgagtaa aattggacgt atttctcgtt
    20101 ttgctggtga tgtaactgga tctagttgga catttgcata tttagattca tctatcgcac
    20161 ccggacaaat tactatttca gagatgaagc gtgtcaaagc attgcttgac gctgactgat
    20221 acgagataac aaaatgttaa atatactgat gagatcccta gcaattgctt agggtttttg
    20281 tcttattaaa acaaacatga gaggacatta gaatgcaggt tgaagttgac ttaccgaatc
    20341 actcttatca tatcaaaatt gaagaaggat gtttttctga ggctggtgac tgggtatcac
    20401 atttatggca gaaacaaatg ataactatta ttacagatag taatgtagag atattatacg
    20461 gggagtcgct tgtcaaccag ttaaaaaaac aaggttttac agtacatgtt tttagttttg
    20521 ctgcgggtga agctagtaaa actttggaag ttgctaatcg tatttatggt tttttagcaa
    20581 agcatcatat gaccagaagc gatgggatca ttgcacttgg tggaggcgtc gtcggagatt
    20641 tagcggcgtt tgttgcttct acttatatgc ggggaatcca ttttcttcaa attccaacta
    20701 gtttaacagc acaggttgat tcttccattg gcggaaaaac aggagtcaac acttcattcg
    20761 ctaaaaatat ggtaggtact tttgcacaac ctgatggtgt actgatagat cctgttactc
    20821 taaaaacatt gggcaatcgt gaattagttg aaggtatggg agaagtcatc aaatatggct
    20881 tgattgatga tataaaactc tggcatattt tagaagagat ggatgggact attgatagta
    20941 tccttgatag tgcattagct attatttatc actcttgtca agttaaacga aagcatgttt
    21001 tagctgatca atatgataaa gggttaagga tgcatttgaa ttttggccac actatcggtc
    21061 atgctatcga agtacatgct ggctatggag aaataatgca tggagaagct gttgcaattg
    21121 gaatgatcca gctttctcgt gtggctgagc gtaaaaatct tatgccaaga ggtatcagtc
    21181 aagatattta taatatgtgt ttaaaatttg gccttccggt ccactatgcg gaatgggata
    21241 aggacgtctt atttgacata ttgagtcatg ataaaaaagc aagtggacaa tttattaaaa
    21301 ttgttatact gcctcaatta ggtagtgcaa cagtccacca aataccttta gaagaaatga
    21361 gagattattt ggagaaatag atgagatatt taactgctgg tgagtcacat ggaccgagtt
    21421 taacagctat tatcgaaggg attcctgcgg gactgaaatt atctgctaaa gatattaatg
    21481 aagatttaaa acgccgccaa ggtggttatg gtcgtgggaa ccgaatgaaa attgaaactg
    21541 atcaagtgat cattagttct ggtgtgcgcc atggaaaaac cttgggttca cctattacac
    21601 tgacagtaac taataaggat catagcaaat ggctggacat catgtcagtc gaagacattg
    21661 aagagcgtct aaagcaaaaa cgtcgtatta agcatcctag acctgggcac gcagatttag
    21721 taggtggaat caaataccga tttgatgatt tgagaaatgc tttagagcgt tcttcggctc
    21781 gtgagaccac aatgcgagta gctattggtg cgattgctaa acgtattttg aaagaaattg
    21841 gtatcgaaat tgccaaccat attgtagtgt tcggtggcaa ggagattact gtccctgata
    21901 agctaacagt tcaacaaatc aaagtgttat ctagtcaatc gcaagtagct atcgtcaatc
    21961 catcatttga acaagaaatt aaggattata ttgacagtgt gaaaaaggct ggagatacta
    22021 tcggaggagt gattgaaact atcgttggag gagttcccgt tggtttaggc tcttatgtac
    22081 actgggatag aaagttagat gctaagatag cgcaagcagt tgtatctatc aatgctttta
    22141 aaggtgttga atttggccta ggttttaagt cagggttttt aaaaggaagt caagtaatgg
    22201 actccatatc atggacaaag gaccaaggtt acatacgtca gtcaaataat ttaggcggtt
    22261 ttgaaggcgg tatgactaat ggagagccta ttatcgttag gggagtaatg aaaccaattc
    22321 ctacgcttta caagccacta atgtcagttg atattgatac tcacgagcct tatagagcta
    22381 cagtggaacg ttctgatccc acggcacttc cggctgcagg tgtcgttatg gaagctgtag
    22441 tagccaccgt cctggtgaca gaagtgctag aaaaattttc ttcagacaac atgtatgaac
    22501 taaaagaagc tgtaaaacta tatcgcaatt atgttaatca tttttaaatg aaaactagta
    22561 gtattcaatt gcaaaaaaag gtataaaagt ctaaaatagt ggtaagttca agtagaaaaa
    22621 ggagtaactt atggctaatg tatatgattt agcaaatgaa ttagaacgtg ctgttcgtgc
    22681 tttaccagaa taccaagcag ttttaactgc aaaagcagct attgaaaatg atgcagatgc
    22741 acaagtgctt tggcaagact ttttggctgc ccaatcaaaa gttcaagaaa tgatgcaatc
    22801 tggccaaatg ccaagtcaag aagaacaaga tgaaatgtct aaacttgggg aaaaaattga
    22861 atccaatgac cttttaaaag tttattttga ccaccaacaa cggttgtctg tctatatgtc
    22921 tgatatcgaa aaaattgtct ttgcacccat gcaggactta atgtaatagt ataaaagaga
    22981 gagctccttg agttttctct tttgtaatat ttcaatttat gatagtctac tgaactagca
    23041 ttgcccacat tatattggtt ttgcattcta cgtagcagaa aattgtgact gtaaacactt
    23101 tgtgatagag tatagttatc aatgattcaa ggagagagta atgtcaaaac aatatgatta
    23161 tatcgttatt ggtggaggta gtgcaggcag tggtaccgct aatagggcag ccatgtatgg
    23221 agcaaaagtc ctgttaattg aaggtggaca agtaggtgga acttgtgtta acttaggttg
    23281 tgtaccgaag aaaatcatgt ggtatggtgc acaagtttct gagacactcc ataagtatag
    23341 ttcaggttat ggttttgaag tcaataatct taattttgat tttactactc taaaagctaa
    23401 tcgcgatgct tacgtgcagc ggtctagaca gtcgtatgcc gctaattttg agcgtaatgg
    23461 ggtcgaaaag attgatggat ttgctcgttt tattgataac catactattg aagtgaatgg
    23521 tcagcaatat aaagctcctc acattactat tgcaacaggt ggacatcctc tttaccctga
    23581 tattattgga agtgaacttg gtgagacttc tgatgatttt tttggatggg agaccttacc
    23641 agattctata ttgattgttg gggcgggcta tatcgcggca gaacttgctg gagtggttaa
    23701 tgaattaggc gttgaaaccc atcttgcatt tagaaaagac catattctac gcggatttga
    23761 tgatatggta acaagtgagg ttatggctga aatggagaaa tcaggtatct ctttacatgc
    23821 taaccatgta cctaaatctc ttaaacgcga tgaaggtggt aagttgatct ttgaagctga
    23881 aaatgggaaa acgcttgtcg ttgatcgtgt aatatgggct atcggccgtg gaccaaatgt
    23941 agacatggga cttgaaaata ccgatattgt tttaaatgat aaaggttata tcaaaacaga
    24001 tgaatttgag aatacttctg tagatggcgt gtatgctatt ggagatgtta atgggaaaat
    24061 tgccttgaca ccggtagcaa ttgaagcagg tcgtcgctta tcagaaagac tttttaatca
    24121 taaagataac gaaaaattag attaccataa cgtaccttca gttattttta ctcaccctgt
    24181 aattgggacg gtaggacttt cagaagcagc agctatcgag caatttggag aagataatat
    24241 caaagtctat acatcaactt ttacctctat gtatacggct gttaccacta atcgccaagc
    24301 agctaagatg aagctcataa ccctaggaaa agaggaaaaa gttattgggc ttcatggtgt
    24361 tggttatggt attgatgaaa tgattcaagg tttttcagtt gctatcaaaa tgggggctac
    24421 taaagcagac tttgatgata ctgttgctat tcacccaact ggatctgagg aatttgttac
    24481 aatgcgctaa cgctatcaaa gaatacaaaa aaagctaggg aacctagctt ttttaaggtg
    24541 ttgttgtatt ttcaatagtt gaggtatcgg tagtagtatt atttgtttga tcattgttag
    24601 tactagatga tgtttgatcg ttattggcag cagaatttga actgctactc ggagtactac
    24661 tagcagagga attttctagt gtttggctgg atgatgccct tgtctcatct ttttcagtag
    24721 aaacttggtt actagaattt tgtccacttt ctcttataat gacggttttt gaagtagaag
    24781 aatcagtctt actagagtct ttgtgattat taatcgcatt agtaatagta atagaggcaa
    24841 caataatact taaaacacta ccgactaatg caattgtttt ttgaagtgca gaaaaacctg
    24901 tttttatatg cttagcgggt gattgagttg aatcagtatt agtcttagta gactttttct
    24961 gttgtcttga atatcctttt gacatgatgc cctccttaaa tgtacatcaa ctattatatg
    25021 attttatcct aaaaaaagct aaagaaaaaa taatttaaaa catttaaata agcaacctag
    25081 gttattaagc tatgttagtg taataggtag tgagttttgt gataaattat gagaattatc
    25141 ataaatattg ttttgggatg ttcacaaact aatataaata gtaggaaaat tatgctataa
    25201 taacactgat tgactttaga aggacagatt tatgatttat tttgataatt cggcaactac
    25261 tattccctat ccagaagttt taaggactta tcaagaagta gctagtaaaa tctatggaaa
    25321 cccctcaagt ttacatgagc taggaacaac atcttctcgg attttagagg ctagccgtaa
    25381 acaaattgct agtttacttg agcttaaagc aaatgaaatt ttctttacct ctggtggtac
    25441 agaggccgat aattgggtta ttaagggtct tgcgtttgag aaacaacatt ttggaaatca
    25501 tattattgtt tctgatatcg aacacccagc tgttaaagag tctgctaaat ggcttggaga
    25561 gtatgggttt gagattgatt atgcgccagt agatgataaa ggatttgtag acgtagaagc
    25621 actggtcaaa ttaattaaac cagaaacgat tcttatttca attatggcaa taaacaatga
    25681 aattggttct attcagccaa taaaagctat ttcagacttg ttgtcagata aacctactat
    25741 atcgtttcat gttgatgctg ttcaagcaat aggtaagata ccgactaaag attatttgac
    25801 tgagcgagtt gattttgcat ctttttctag tcataaattt cacggcgttc gtggggttgg
    25861 ctttctatat attaaagaag gaaaacgtat ttcaccatta ctaacaggtg gtggacaaga
    25921 aactgattta cgttcaacta ctgagaatgt cgcagggatt gcagctactg ctaaagcttt
    25981 acgaatggtt atggacaagg aagtagtagc tattcctaaa ataagtaaaa tgaaaaccat
    26041 tattcatgat gaattagcca aatatgagga tattacgtta ttttcgggta aggaagattt
    26101 ttcacctaac attatcacgt ttggtatcaa gggtgtccga ggagaagttc ttgtccatgc
    26161 ctttgaggga catgatattt ttatctcgac aactagcgct tgctcttcta aagcaggtaa
    26221 accagcagga actcttatag caatgggaat ttcaactaag ttagcccaaa ctgctgtacg
    26281 tatcagctta gatgatgata atgatatggg acaagtagag caatttttaa ctatttttaa
    26341 acaagtttat gaaaaaacta agaaagttag aggataagat gcagtattca gaaattatga
    26401 ttcgttatgg agaactctct actaagaaga aaaaccgtat gcgcttcatc aataagttaa
    26461 aaaataatat ggagcatgta ctctccattt atccagatgt ttcagtaaaa acagatcgtg
    26521 atagaggaca tgtatatctc aatggtacag attatcatga agttgcagag tccttaaaag
    26581 agatttttgg tatccaagct ttttctccat cttttaaagt agaaaaaaat gttgatacat
    26641 tggtaaaagc tgtccaggaa attatgactt ccgtttataa agatgggatg acttttaaaa
    26701 ttaccgcaaa acgtagtgac cactcatttg aattggatag ccgtgctcta aatcatactt
    26761 taggagatgc tgttttttca gtcttgccaa atattaaggc tcagatgaag caaccagata
    26821 tcaatcttaa agtcgagata cgagatgagg ctgcttatat ttcatatgag gatattaggg
    26881 gtgcaggagg attaccagta ggaacatctg gaaaagggat gctgatgttg tctggtggga
    26941 ttgattctcc ggtagcaggt tacctagcgt taaaacgtgg tgtagatata gaagcagtcc
    27001 attttgcaag tcctccttat actagcccag gtgcattgaa aaaagcacat gatttaacac
    27061 gtaaattgac aaaatttggt ggtaatattc aatttattga agttccattc acagaaattc
    27121 aagaggaaat taaggcaaaa gctcccgaag cctacttgat gacgttaaca cgtaggttta
    27181 tgatgcgtat tacagatcgt attcgtgagg accgaaatgg tcttgttatt attaacggtg
    27241 aaagtttagg acaggtggca agccaaacgt tagaaagtat gcaagctatt aatgctgtca
    27301 ctgcaacacc gattattcgt cctgtggtca cgatggataa gctagaaatt attgatattg
    27361 ctcaaaaaat agatactttt gatatttcaa ttcaaccatt tgaggattgc tgtacgattt
    27421 ttgcaccaga tcgcccaaaa actaacccta aaattaagaa tacagaacag tatgagaaac
    27481 gtatggatgt agaaggtctt gtagagaggg cagttgcagg gattatggta actactattc
    27541 aacctcaagc agatagtgat gatgttgatg acttgattga cgatttatta taatgaattt
    27601 ttaaactaca gatttactct gtagtttttt attaaaatga ttgcgctatc ataaggttat
    27661 aaaaaatgct ataatacgaa taaaggaggc aagtatggca agaatccctt atcagaaaac
    27721 acttattttt tgtgtagccg ttattattgc tatttttatt ttggggttat ctaaggattt
    27781 agcacaatct aaagggcaaa aggttgcaaa taataatact gtaaaaacag cacgtgtggt
    27841 tgcaaacgga gatattttgc tgcacgatgt actatatgcc agtgcgcgcc aacccgacgg
    27901 cacttataat tttactcctt attttaaaga ggtaaaatct tggattgaga gcgctgattt
    27961 agcgattggt gattatgaag ggacgattag ttcagaatat cctttagcgg gctatccttt
    28021 atttaatgct cctaatgaga ttgcaactac tatgaaagaa actggatatg atgtcgttga
    28081 cttagcacat aatcatattc tggattcaca actggcaggt gctatcaata ctgtcaagac
    28141 ttttaatcgt ctgggattgg atactattgg ggtttattta aaggatagaa ataaagaaga
    28201 tatcttaatt aagcatgtaa atggtattaa aattgctatt ttaggttatt cgtatggcta
    28261 caatggtatg gaagctaatg tttcaaagag tgactatgaa aaacatatgt ctgatttaga
    28321 tactaaaaag attaagcaag atattaaaaa ggctgaaaaa gaagcagata tcaccattgt
    28381 catgccacag atggggatag aatatcaaaa aaaacctact acagaacaag taatgcttta
    28441 tcatagtatg attaaatggg gggcagatat tatattcgga ggacaccctc acgttgttga
    28501 accgtctgaa gtgataaaaa aagatggcca gaaaaaattt attatttact ctatgggaaa
    28561 ttttatttca aatcagcgtt tagaaacggt tgatgatatc tggacagaaa gaggattatt
    28621 aatggatgta accatagaga aaaaaggtca aaaaacagta attaaaaaag ttaaggcgca
    28681 tccaacacta gtagaagcaa aaccaaatgg ccgttatgtt gaagaaggct ttccagctta
    28741 tgattatagg acgctagttt tagaggatta tattgaaggt ggtaagaaac gttctacgat
    28801 tgatcagact atgaaagata aagttgataa agcatataag gagatgaatg agttagttaa
    28861 tttgaagtgg taaagttgat aattttattt tgttttatct tgcataaaaa aagagtttat
    28921 gataaaattt gaatgttgac taaatgcaca tcctgtgcaa ccgcgcgaaa atcgttagat
    28981 taagtagagc aaggctctca cgatttatag gcgagtcttc acagagggaa acctcacaag
    29041 aaatttttat aggaggtgca tcatgagcac atacgcaatc atcaaaactg gtggtaaaca
    29101 agttaaagtt gaagtaggac aagctatcta cgttgaaaaa cttgacgttg aagcaggagc
    29161 agaagttaca tttaacgaag ttgttcttgt tggtggtgaa acaactaaag ttggtactcc
    29221 agttgttgaa ggagctactg ttgttggtac tgttgaaaaa caaggcaaac aaaagaaagt
    29281 tgtttcttac aaatacaaac ctaaaaaagg tagccaccgt aaacaaggtc accgtcaacc
    29341 ttatacaaaa gttgttatca acgcaatcaa cgcttaattt ttcatgatta aagcaacttt
    29401 tactcgtaat caatcaggct atctttatag tgctgaaatc agcggtcatg ctggtagtgg
    29461 cgagtacggc tttgatgtga tttgtgcagc tgttagtact ttatcaatta attttattaa
    29521 ttcattagag gctttaacaa cctgccaagc gcaattgatt atcaatgacg ttgaaggtgg
    29581 ttatatgaag atagatttat cttctatacc acaacataaa gaggataagg ttcaattatt
    29641 atttgagtct taccttcttg gaatgactaa tctgtctaaa gattcttctg aatttgttag
    29701 tacagtagtc atgacacact aatttaagag aggacacaac attatgttaa aaatgaatct
    29761 tgctaacttg caacttttcg cccacaaaaa aggtggaggt tctacatcaa acggacgtga
    29821 ttcacaagca aaacgtcttg gagctaaagc agctgacgga caaacagtat caggtggatc
    29881 aattctttac cgtcaacgcg gtacacacat ctatccaggt gctaacgttg gacgtggtgg
    29941 ggacgataca ctttttgcta aagttgaagg tgttgttcgt ttcgaacgta aaggacgcga
    30001 taaaaaacaa gtgtctgttt acccaatcgc aaaataatgc ctagttaaga ctttatgact
    30061 attcataagg tcttttatta ttgtcagagc tttcattttg taacaaaaga caatatgtta
    30121 agagatttat cattaatact cataaacttt gttgttcttt tatagtgaaa tagtcctaat
    30181 tcatgaagaa ttttccgcaa ggatagttat tttttggtat aatgaagggt actaaaatga
    30241 ggaagtgata aaatgaatat tcaacaactg cgttatgttg ttgctatcgc aaatagtggt
    30301 acttttcgtg aagcagctgc aaaactattt gttagtcagc caagtttatc tgttgccgtt
    30361 cgtgatttgg aaacagaatt agggtttcag atttttacgc gtacaactac tggtgcagtg
    30421 ttgaccaatc aaggcatgac tttttatgag aatgctttag aagttgtgaa aagctttgat
    30481 tcattcgaaa aacagttttc gcaatctgaa gctacagagc aagaattctc aatagcaagt
    30541 caacattatg attttctacc tcctttaatc acagcgtttt caaaatgtaa tgataatttt
    30601 agctatttcc gtattttcga atcaacaaca atacgtatct tagatgaagt tgctcaaggg
    30661 aatagcgaaa taggtatcat ttatatcaat agtcaaaata aaaaaggtct acttcagcgg
    30721 ttggacaaat tggggttaga gtttgtagag cttattcctt ttaaaactca tatttatctt
    30781 gggaaagacc atcccttggc tagtaaaaca tctctgataa tgacagattt agaaggatta
    30841 ccaaccgtac gttttacaca ggatagagat gattatcggt attactcgga gaattttgtg
    30901 gaagtacttg atagttctgt tacctataat gttactgata gagcaacatt aaatggtatt
    30961 ttagaaagaa cccaagctta tgcgacgggc tcaggattct tggatagccg cagtgtgaat
    31021 ggtatcactg ttattccatt agaagatcat ctagataacc aaatgattta tatcaaacga
    31081 aaagatcgta atttatctca gatggcttta aaatttgttg ctgttatgga agagtatttt
    31141 aagggattta attcatagga gaagtaatga gaaagattat tataccaata ataactattt
    31201 tgttgattgc tttagatcaa ctatctaagt tatggattgt taaacatatt gaactaaacc
    31261 aaattaagga attcattcca aatatcgtta gtctaactta tctgagaaat tatggtgctg
    31321 cattttctat tttgcaaaat caacaatggc tttttacctt aattacaatc tttgttgttg
    31381 gtgttgctat tatttatttg atgaaacata ttaatggaag ttattggtta ttaatttctc
    31441 taaccttaat catctctggt ggattaggaa actttataga tcgcttaaga ttaggttatg
    31501 tcgttgatat ggtgcattta gattttatta attttgctat ctttaatgta gcagactcat
    31561 atctgacaat cggtattatt tgcttaatga ttgcactttg gaaagaggaa agtaatggaa
    31621 atcacaatta aaatagcagg agttaggcta gataaagctt tagcggataa ttctgaactg
    31681 tcgcgtagtc aagctaatga agaaattaaa aaagggattg tgttagtaaa tggacaagta
    31741 aaaaaagcga aatatactgt tcaagaagga gatcggatta cttttgatat tcctaaagag
    31801 gaagtactag attatcaagc tgaaaatatc cctttagata ttatctatca agatgatgac
    31861 gttgctgtgg ttaataagcc gcaagggatg gttgttcacc cttctgcagg ccacagttcg
    31921 ggcaccttag ttaacgcctt gatgtatcat attaaggatt tatcatcaat taatggcgtt
    31981 gttcgcccag ggatcgttca tcgtattgat aaagatactt ctggtttatt gatggttgca
    32041 aaaaatgata gagctcacca agtgttggct gaagagttaa aagataaaaa atcgcttcgt
    32101 aaatatttag caattgtgca tggtaattta ccaaatgatc gaggtgtgat tgaggctccg
    32161 attggtagaa gcgataaaga tcgtaagaag caagccgtga ctgctaaagg taagccagca
    32221 ataactcgtt ttcatgtttt agaacgtttt ggtgattata ccttagttga attgtcattg
    32281 gagacaggtc gaacacacca aattcgggtt catatggctt atattggcca tcctctagcc
    32341 ggtgatcctg tctacggtcc ccgtaagact ttagggggta aaggacagtt tttgcatgca
    32401 caaacactag gttttacgca ccctagtaac ggagagaatt tgattttttc agtagaggtt
    32461 ccggaaattt ttcagacaac cttagaaaag ttgagaaaaa attaagtttt tcttgaaaaa
    32521 tacttttacc atttgctata atataagcaa atcattcctt taaccttatc cagtgagata
    32581 gttaaggaga aacttcagac taaatcaatg cctgagcggc tttgtcattc tagagagtct
    32641 ccttttgaag gggctctttt atcttagaga ggatactgca atgaaaagaa aagaaattat
    32701 tgatgatgtg accatgaaac gtgctattac acgtattacc tacgaaatta tagaacgtaa
    32761 caaaaattta gataatattg tcttagcagg cattaaaaca agaggtgttt ttttagccaa
    32821 acgcattcaa gaacgcttga agcaattaga aaatttagat attcccgttg gagaattgga
    32881 tactaaacct ttccgtgatg atatgaaagt tgaagttgac accacaacta tgccagttga
    32941 catcactgat aaagatatta tattaattga tgatgttctc tatacaggtc gcactattcg
    33001 agctgctatt gataatcttg ttagtttagg gcgtccgagt cgtgtcagtt tagctgtatt
    33061 aattgaccgt gggcatcgtg aactacctat tcgtgcagat tatgtgggga aaaatattcc
    33121 aacaagtcaa tttgaagaaa ttctagttga agtgatggaa catgatggtt acgatcgtgt
    33181 gagtattatt gatccgaatt aagtagacga ctattattta cgatgatttt atagaagggg
    33241 aagaaccaca atggcaaaaa aattgttaat tctagaggat ggtacggttt ttgaaggact
    33301 atcctttggt tcatctcttg atgtaactgg cgaattagtt ttttgtacgg ggaataccgg
    33361 ctatcaggaa ataataacga atccttcaca taatggaaaa attttagttt ttacctcacc
    33421 gctaatagga aattatggta ttcatcgcag ttactctgaa gccattattc ccacttgttt
    33481 aggagttgtt gttgcagagt attccagatg tgtaagtagc gatactagca agatgaatct
    33541 agatgaattt ttgaaaatga aaaaggttcc tgcaatgtcg ggggtcgata caaggtattt
    33601 gatgcaagtt attaaagaaa aaggatttgt aaaagctact ttagcagaag caggtgatgt
    33661 tttatcacat ttacaggatc aattaatagc gacagtatta ccgacaaata atgtggaaca
    33721 agtttcgaca aaaacagctt atccgtcgcc tgctagcggt cgaaatattg ttgtacttga
    33781 ttttggtctt aaacattcta ttttgagaga attgtctaaa cgtcagtgtg acgtgactgt
    33841 cattccctac aatacgtctt tagaaggtat taaaaatcta tatccagaag gtatcatatt
    33901 atctaatggt ccggggaatc cagaaaagct tcaggaaatc ttaaacacta ttaaagaatt
    33961 acaaaaatct gttcctatgt taggtattgg tttggggcac caattaattg ccatggcaaa
    34021 tggtgcagaa attatgagac tcccagttgc taagaagggg ccaaattatc ccatgagaga
    34081 tattgctacg ggtcgtttag aaacagttag tcagtttaat cattttaccg ttaaccggct
    34141 aaatcttccg catgatttac tggtaacaca cgaaggtttg aatgatcaag agattgttgc
    34201 tctaaggcac cgctcatttc cagtgatgtc cgtgcaattt tacccagagg ctgctccagg
    34261 ccctcatgat gtgacctatt tttttgatga atttttagaa atgattggat cacgcgctta
    34321 aacataaggt attatttaaa gtcagtcggt gagctgatta ataaggaggt gcgtatatgc
    34381 taaatacaaa ggataaacag cctattttgc ttattatttt cggacagaaa atgaaagaat
    34441 atgacgattt aattagatca actgttgaga ggttatctaa aagggataga cctgttagta
    34501 ttcttatctc gaaggagcta aaccatgtga ttcaccatta tgatgcacaa tttgaaaatc
    34561 ttgagacgct tgtggggtat ataaatacac atgaagatag tttgatttac cccctttgct
    34621 tagataaaaa tcatgagcta tttcaagagt ttttagcgtg gcagagtcat tattcaaggc
    34681 agtatatcag cgataattta tcagaaaaaa gtgttcaggc aatagacaat aatcaagact
    34741 ttgtgcaagt agattgttta gtaatgagag actctttgaa taattgttta tatgtctctg
    34801 atttagaata tattgaatct aataaaacaa ctggtaagtc tctagcgatt gtaccaagtc
    34861 aaactttatc cgatgctgct aggcaaacaa ttcgagatgt tgcatttgat gtgtgtcgaa
    34921 aagcaaacat tattggagta tgttacttta gtttcttaat agatttgaac agtttggatt
    34981 atcatataat tagtttatca tctggtttgt ctcatcaaag cattttattt gagactatta
    35041 cgacctatcc tgttttagag atagcaacta aattgacagt gggatatact tttagtcaac
    35101 tgaagcattc ttattaccca aatacctctg cctttttaga gccgcaatta gactatgtag
    35161 ctaccgtatc ttttagtttt gaaaaagttg actatatctt ttttgctcgt aatatagagc
    35221 aattattttt aaatttactc gaggctagca gtcatgatca ttttccattt ctgagtgata
    35281 ttagcgagga agatttgatg tttgctttga ttcagaaaaa agaaaatcgt ctagcttatc
    35341 ttttggaggc ttttagacgt ggctttgatt tgtatgattt atcctcagtt actaaaatca
    35401 acccttttta cttagataaa tgtttacata ttgtagagtt gtatgaaaac ctgaataaaa
    35461 gccaatataa tgtagatatt tataaggaag ctaaaaggta tggtttttcg gatgactata
    35521 ttgctagtag ttggcaaatc tctttgatag acatgttaga atatcgaaaa aaacatagtg
    35581 tcgcacctgt tctgaagcaa gtcgaacaaa gttctggtgt tctaactggc catcaaattc
    35641 agtattttcg aagctatgat tggcattcag actacatttc tagcggttgt caaaaggctt
    35701 tgataatggt agacaagggt tattccctag taaaattaaa tgagttaatt aagcaaatca
    35761 aacaaactca tttagaatta ttaattgtta ccaatcaacc tctcctcata gaacaattaa
    35821 atgatacatc tataattttt gacactatag gtatt
//
LOCUS       DATRTN010000013        33762 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.013, whole genome shotgun sequence.
ACCESSION   DATRTN010000013 DATRTN010000000
VERSION     DATRTN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33762
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.013"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(18..869)
                     /locus_tag="VBE22_000676"
     CDS             complement(18..869)
                     /locus_tag="VBE22_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7202150.1"
                     /translation="MDFKELFPEVITKQEVKQSEDYIIVEQDGHVLHFPKSSLTKREL
                     YLLQMTPSLEDASSVDSQNPWYRYLVEGRGRLPQSHSAVQFIFIEHQFTLSEELKDFL
                     SPLVINVETIMTINQTQSVMILNQDNFFNATELLTDILPTIENDFNTRLRCYFGNSWT
                     HLQAVDWKELYEEEYKLFTLFLSHKAEQHYCRFPKMALWALANQSPMPSIKAKCLQHI
                     LDTSDTSAIIKALWQEQGNLAKTAKALFIHRNSLQYKLDKFTQSSGLNLKILDDLAYA
                     YLISMDT"
     gene            complement(964..1860)
                     /locus_tag="VBE22_000677"
     CDS             complement(964..1860)
                     /locus_tag="VBE22_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEO7202151.1"
                     /translation="MAITIQNHELQVTLKALGATMTSITDSQGVEYLWQGDATYWGGQ
                     APILFPICGSVRNDCVIYRPAQAPHFTGIIPRHGFVRHKTFDYDYISDSSVRFTIKSS
                     KEMLINYPYRFSLEITYTLRNKSIAITYIVKNLESEKNMPYAIGAHPGFNCPLFEKEV
                     FSDYYLEFEQFETCTIPESFPDTGLLDLQARHPFLENQKQLSLNHALFEKDAITLDQL
                     RSKTVYLKSRNHAKGIQLDFDDFENLILWTSNNGGPFLALEPWSSLSTSIEESDILED
                     KQNIVRLNPKQSKQHSIRITIL"
     gene            complement(1914..2891)
                     /gene="lacD"
                     /locus_tag="VBE22_000678"
     CDS             complement(1914..2891)
                     /gene="lacD"
                     /locus_tag="VBE22_000678"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEO7202152.1"
                     /translation="MGLTEQKQKHMEQLSDKNGIISALAFDQRGALKRLMAKYQSEEP
                     TVSQIEALKVLVAEELTPYASSMLLDPEYGLPATKVLDDNAGLLLAYEKTGYDTSSTK
                     RLPDCLDIWSAKRIKEEGADAVKFLLYYDVDSSDEVNEEKEAYIERIGSECVAEDIPF
                     FLEILSYDEKITDSSGIEYAKIKPRKVIEAMKVFSNPRFNIDVLKVEVPVNMDYVEGF
                     AQGETAYNKATAAAYFREQDQATLLPYIFLSAGVPAQLFQETLVFAKEAGAKFNGVLC
                     GRATWAGSVKEYVEKGEAGARQWLRTIGFQNIDELNKILQKTATSWKER"
     gene            complement(2893..3825)
                     /locus_tag="VBE22_000679"
     CDS             complement(2893..3825)
                     /locus_tag="VBE22_000679"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEO7202153.1"
                     /translation="MILTVTLNPSIDISYCLENFNMDTVNRVTDVSKTPGGKGLNVTR
                     VLSQLGDNVVATGLLGGDFGDFIRSGLDALEIRHQFLSIGGETRHCIAVLHEGQQTEI
                     LEKGPHITKDEADAFLNHLKLIFDAATIITVSGSLPKGLPSDYYARLISLANHFNKKV
                     VLDCSGEALRSVLKSSAKPTVIKPNLEELTQLIGKPISYSLDELKSTLQQDLFRGIDW
                     VIVSLGARGAFAKHGNHYYQVTIPKIEVINPVGSGDATVAGIASALEHQLDDTNLLKR
                     ANVLGMLNAQETLTGHINLTYYQELISQIQVKEV"
     gene            complement(3836..4351)
                     /gene="lacB"
                     /locus_tag="VBE22_000680"
     CDS             complement(3836..4351)
                     /gene="lacB"
                     /locus_tag="VBE22_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEO7202154.1"
                     /translation="MKIAVGCDHIVTYDKIAVVDYLKTKGYEVIDCGTYDNIRTHYPI
                     YGKKVGEAVASGKADLGVCICGTGVGINNAVNKVPGIRSALVRDLTSAIYAKEELNAN
                     VIGFGGKITGGLLMTDIIEAFIRAKYKPTKENKVLIEKIAEVETHNAHQEENDFFTEF
                     LDKWNRGEYHD"
     gene            complement(4372..4797)
                     /gene="lacA"
                     /locus_tag="VBE22_000681"
     CDS             complement(4372..4797)
                     /gene="lacA"
                     /locus_tag="VBE22_000681"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEO7202155.1"
                     /translation="MTIIIGADAHGVELKEVIRQHLTSLGKEIIDLTDTSKDFVDNTL
                     AIVAKVNQKEDNLGIMVDAYGVGPFMVATKVKGMIAAEVSDERSAYMTRAHNNARMIT
                     LGSEIVGPGVAKHIVEGFVDGTYDAGRHQIRVDMLNKMC"
     gene            complement(5045..7495)
                     /locus_tag="VBE22_000682"
     CDS             complement(5045..7495)
                     /locus_tag="VBE22_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEO7202156.1"
                     /translation="MTLTFGKPSITIRRLGVITSTYMMLGFILLCGFLHPSTIYSEEI
                     KSDINSFISIDDEKTTVPIFDSTLDKLTNSIEERQLELDSDTSDSLIVDDQKDKDMVI
                     VLGDSDSQEKLSPPLKSEIATEENRKQANLAELNATQPNNRTTYIIPESSHSIAEQQR
                     FLIESKGYSVALLNSDEFRKTAREDRGFERDKLRSLDIIPKGDLSTSNVIGNTDIASQ
                     ISLGFKKNAIQEHHLTKTFSQKDGKLSSVIEGMLAIGKEKVEKEIKYSGNLWQKLKAK
                     AHCLVCCVDNLNFEDIKSYFQYYCHLNHQLKLPKGAILSAKTEVYRGGDFGRKNKDNV
                     FGYRIPSLLKTEKGTLLVGADERIEQACDWGNIGMVIRRSEDDGVTWGKRETIVNLRN
                     NPRVPLVTSGDYSGSPINMDMALVQDTSSKTKRIFSIYDMFPEGRGVISIANTPEKEY
                     TQIGGQSYLNLYNNGKKSKVFTIRDKGIVYNFKGKKTDYHVITETTKSDHSNLGDIYK
                     GKQLLGNIYFTKHKTSPFRLAKSSYVWMSYSDDDGRTWSSPRDITASLRQKGMKFLGI
                     GPGKGIVLKWGPHAGRIIIPAYSTNWKSHLRGSQSSRLIYSDDHGKTWHTGKAVNDNR
                     ILSNGEKIHSLTMDNKKEQNTESVPVQLKNGDIKLFMRNLTGNLEVATSKDGGETWQN
                     HVKRYKEVHDAYVQLSAIRFEHDKKEYILLVNANGPGKKRQDGYARLAQVNRNGSFKW
                     LYHHHIQDGSFAYNSVQQLNNDKFGVLYEHREKHQNSFTLNYKVFNWSFLSQNTEKQG
                     TLWEKMAANWHVLFKFYL"
     gene            complement(7734..9182)
                     /locus_tag="VBE22_000683"
     CDS             complement(7734..9182)
                     /locus_tag="VBE22_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO7202157.1"
                     /translation="MQVFLNIVNKFFDPIIHMGSGVVMLIVMTGLAMIFGVKFSKALE
                     GGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGINLSIIDVGWAPLATITWGSPYT
                     LYFLLIMLIVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYAKKNNLPTLLSVIIA
                     TVAIIFVGVLKIINSDLMKTTFDDLLGTGPTSPMTSTHMNYMMNPIIMVLDKLFDKVF
                     PGLDKYDFDAAKLNKAIGFWGSKFFIGMILGLVIGIMGNPVFSFAALGGWFSLGFTAG
                     ACLELFSLIGSWFIAAVEPLSQGITNFANGKMHGRRFNIGLDWPFIAGRAEIWACANI
                     LAPIMLVEAILLSKVGNGILPLAGIIAMGVTPALLVVTRGRLIRMITFGTLLLPLFLL
                     SGTMIAPFATELAKKVGAFPAGARAGSLITHSTLEGPMEKIFGYVIGKATTGQLSAII
                     TLIIFATAYLGLFMWYAKQMKRRNAEYAANQK"
     gene            complement(9222..9527)
                     /locus_tag="VBE22_000684"
     CDS             complement(9222..9527)
                     /locus_tag="VBE22_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7202158.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIETQLGDRGYNVHCDAVMVKDIT
                     EEMVNKYDIFTPIAKTDLGFNVPIPVVEAGPILYRIPVMSEPVFTALEQVIKEHNLS"
     gene            complement(9529..10002)
                     /locus_tag="VBE22_000685"
     CDS             complement(9529..10002)
                     /locus_tag="VBE22_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7202159.1"
                     /translation="MFAVTQDILFIDAHSQEELFDLVSKALIKQHYVSPNYRQAVKER
                     EREFPTGLKIDLKDGTPIQYVAIPHTETQYCLVDRIFYVKNSQPITFKHMINPEEECR
                     VQDFFFIINSRNSNQSDILSNLITFFITKGNLDRLHELGDNKEKINHYLIEKGVF"
     gene            10212..10988
                     /locus_tag="VBE22_000686"
     CDS             10212..10988
                     /locus_tag="VBE22_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO7202160.1"
                     /translation="MLKRERLQKIIEKVNINGIVTVNEIMEELDVSDMTVRRDLDELD
                     KAGLLIRIHGGAQKVNASPTPQNYEKSNTEKYDIQTNEKLEIAQFAKQFINDGETIFI
                     GPGTTLEKLATQLLDFKIRVVTNSLPVFNILNQSSTLDLILVGGEYREITGAFVGSVT
                     INSIKSLNFSKAFVSSNGVFEKSIATYDEGEGEIQRIALNNSFEKFLLVDSQKFGKYD
                     FYTFYQLDDIDFVLTDHNIDNVVKEQYSSFTKILTNNNYM"
     gene            complement(11270..13851)
                     /locus_tag="VBE22_000687"
                     /pseudo
     CDS             complement(11270..13851)
                     /locus_tag="VBE22_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080375.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(13878..14801)
                     /locus_tag="VBE22_000688"
     CDS             complement(13878..14801)
                     /locus_tag="VBE22_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEO7202161.1"
                     /translation="MKRIRKSLIFVLGVVTLICLCACTKQSQQKNGLSVVTSFYPVYS
                     ITKAVSGDLNDIKMIRSQSGIHGFEPSSSDVAAIYDADLFLYHSHTLEAWARRLEPSL
                     HHSKVSVIEASKGMTLDKVHGLEDVEAEKGVDESTLYDPHTWNDPVKVSEEAQLIATQ
                     LAKKDPKNAKVYQKNADQFSDKAMAIAEKYKPKFKAAKSKYFVTSHTAFSYLAKRYGL
                     TQLGIAGVSTEQEPSAKKLAEIQEFVKTYKVKTIFVEEGVSPKLAQAVASATRVKIAS
                     LSPLEAVPKNNKDYLENLETNLKVLVKSLNQ"
     gene            complement(15056..15499)
                     /locus_tag="VBE22_000689"
     CDS             complement(15056..15499)
                     /locus_tag="VBE22_000689"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEO7202162.1"
                     /translation="MKIIIQRVNQASVSIEDDVVGSIEKGLVLLVGIAPEDTTEDIAY
                     AVRKITSMRIFSDDEGKMNLSIQDIKGSVLSISQFTLFADTKKGNRPAFTGAADPVKA
                     NQFYDIFNQELANHVSVETGQFGADMQVSLINDGPVTIVLDTKNK"
     gene            complement(15509..17725)
                     /locus_tag="VBE22_000690"
     CDS             complement(15509..17725)
                     /locus_tag="VBE22_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEO7202163.1"
                     /translation="MVKEINLTGEEVVAITSQYMSETDVAFVKFALNYATAAHYYQAR
                     KSGEPYIIHPIQVAGILADLHLDAVTVACGFLHDVVEDTEITLDEIETDFGKDVRDII
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLSFRYLNETEFYKISHMMSEKRREREELV
                     DIIVDKIRSYTEEQGLYGDIYGRPKHIYSIYRKMRDKKKRFDQIYDLIAIRCIMETAS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKEMHQV
                     AEFGVAAHWAYKKGITSKVNQAEQSVGMGWIQELVELQDESKDAKDFVDSVKEDIFTE
                     RIYVFTPNGAVQELPRESGPIDFAYAIHTQVGEKATGAKVNGRMVPLTAKLKTGDVVE
                     IITNPNSFGPSRDWIKIVKTNKARNKIRQFFKNQDKETSINKGRELLVDYFQEQGYVP
                     NKYLDKKHIEEILPRVSVKSEEALYAAVGFGDLSPISIFNKLTEKERREEERAKAKAE
                     ADELINGGEIKTDKRDVLKVKSENGVIIQGASGLLMRIAKCCNPVPGDLIEGYITKGR
                     GVAIHRSDCQNLKSQENYEQRLIDVEWDDDGSKKEYMAEIDIYGLNRSGLLNDVLQTL
                     SNATKLVSTVNAQPTKDMKFANIHVSFGISNLAQLTTVVDKIKIIPDVYSVKRTNG"
     gene            17985..20387
                     /locus_tag="VBE22_000691"
     CDS             17985..20387
                     /locus_tag="VBE22_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HEO7202164.1"
                     /translation="MSKHYFSKSVFALTVLTATATSGLAVQAEDIVTTPSSTSTKVES
                     TTPTSTIAEEKSNVTSTPTAITDASTATSTNTTTNNQNPQPVATEATTSDLKPIEGEK
                     VDLRIMSTTDLHTNLVNYDYYQDKESQKIGLAKTAVLIEEAKKENPNTVLVDNGDVIQ
                     GTPLGTYKAIVKPVAENEEHPMYQAMNALGYDASTLGNHEFNYGLDYLKKIIATANLP
                     ILNANVLDFKTHQPVFKTYDIITKTFKDSTGRAVALNIGITGIVPPQILNWDKANLEG
                     KVIVKDSVKAIEEIVPTMRAKGADVILVLSHSGIGDDRYEEGEENVGYQIASIKGVDA
                     VVTGHSHAEFPSGNGTGFYEKYTGVDGINGKINGTPVTMAGKYGDHLGIIDLGLSYTN
                     GKWQVSESSAKIRKIDMNSTTADERIIALAKEAHDGTINYVRQQVGTTTAPITSYFAL
                     VKDDPSVQIVNNAQRWYVANELKGTPEANLPLLSAAAPFKAGTRGDATAYTDIPAGPV
                     AIKNVADLYLYDNVTALLKVTGADLREWLEMSAGQFNQIDPNNKAPQNIINTEYRTYN
                     FDVIDGLTYKFDITQPNKYNKDGKVVNSQASRVRDLMYNGKPVADKQEFMIVTNNYRA
                     SGTFPGAKNATMNRLLNLENRQTIINYIISEKTINPTADNNWGFTESIKDLDLRFQTA
                     DKAKNLVTNSKDIQYIASSTKDEGFGDYRFVYTEQEKVDLTEPSHQEKLSQIEKQKIH
                     HMVATAYTKAKASPNITGTVYTQQKEKTLPKTGQEKQSYLAFFGVAILGLVGSLLKKK
                     QI"
     gene            20544..20999
                     /gene="nrdI"
                     /locus_tag="VBE22_000692"
     CDS             20544..20999
                     /gene="nrdI"
                     /locus_tag="VBE22_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO7202165.1"
                     /translation="MPKLTLVYISLSGNTQSFVKRLSEQLSTWHNCQVSTINIKEQNH
                     QTFPVDQPFVALLPTYLEGGNGIDNGDQEILTIPLGDFIAAHNNVKNCIGIVGSGNRN
                     FNNQYCLTAKQYSERFGFPMLGDFELRGTSSDVERIANIIVETLSNFKA"
     gene            21010..22047
                     /locus_tag="VBE22_000693"
     CDS             21010..22047
                     /locus_tag="VBE22_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEO7202166.1"
                     /translation="METTLNYIKTLTSIPSPTGFTQTIMTYIIKELEAFGYSPIRTNK
                     GGVMVSLKGKNDTKHRMITAHLDTLGAMVRAIKPDGRLKIDLIGGYTYNAIEGENCTI
                     HLSKNGKEISGTALIHQTSVHVYKDAGTAERNQTNMEIRLDEKVTTADETRALGIQVG
                     DFISFDPRTIITDSGFIKSRYLDDKVSAGILMELLSVYKKEDIQLPYTTHFYFSAFEE
                     LGHGANSSIPNETVEYLAVDMGAMGDDQETDEYTVSICVKDASGPYHYELRQHLVSLA
                     ENNNIPYKLDIYPYYGSDASAAMRAGAEVKHALLGAGIESSHSYERTHIDSIQATELL
                     VDAYLKSNMVD"
     gene            22047..22544
                     /locus_tag="VBE22_000694"
     CDS             22047..22544
                     /locus_tag="VBE22_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO7202167.1"
                     /translation="MVATLIIIRGNSASGKSTIAKQLQAELGENTLLLSQDYLRREML
                     GTKDGENTTTIPLLINLLNYGYHNCSYIILEGILRSDWYTPVWKHILKHNPNNTYAYY
                     YDLSFQETVKRHSTRLKSLEFGEDSLARWWLEKDFLKEIPEKILTKAMSLEDVKKLIM
                     TDVLP"
     gene            complement(22766..23803)
                     /locus_tag="VBE22_000695"
     CDS             complement(22766..23803)
                     /locus_tag="VBE22_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO7202168.1"
                     /translation="MKEKQSKRLIYILLVVSIIFISVFTYSISQPSKLLPPKELVILS
                     PNSQAILTGTIPAFEEKYGIKVKLIQGGTGQLIDRLSKEGKQLKADIFFGGNYTQFES
                     HKALFESYVSKNVHTVIPDYIHPSDTATPYTINGSVLIVNNELAKGLTIKSYEDLLQP
                     SLKGKIAFADPNTSSSAFSQLTNILLAKGGYTNPKAWNYVKKLQHNINAIKSSSSSEV
                     YQSVAEGKMIVGLTYEDPSVNLQKSGANVSIVYPTEGTVFVPSSVAIIKNAPSMKEAK
                     LFINFMLSLDVQNAFGQSTSNRPIRKDAQTSNGMKALKDIATLKEDYRYVTKHKGQIL
                     KTYNRIRRNAD"
     gene            complement(23815..24588)
                     /locus_tag="VBE22_000696"
     CDS             complement(23815..24588)
                     /locus_tag="VBE22_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO7202169.1"
                     /translation="MYRLLIVEDEHLIRKWLRYAIDYQSLNILVVGEAKDGKEGAQLI
                     QEEQPDIVLSDINMPIMTAFDMFEATKGQSYAKIILSGYADFPNAQSAIHYGVLEFLT
                     KPLEKQALIDCLKTIMARIEEHKEKHLQEHTELYLPLPQANDQVPEVIKDMLAWIHSH
                     FHGKIVISQLAHDLGYSESYLYTVTKKHLHITLSDYINQYRINQAIQLMFREPDLMVY
                     QIAEAVGIYDYRYFDRVFKKYLGQTVKAFKEEHIFKQMD"
     gene            complement(24592..26241)
                     /locus_tag="VBE22_000697"
     CDS             complement(24592..26241)
                     /locus_tag="VBE22_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7202170.1"
                     /translation="MRGYRMEERFKKRLQDDISKHFSRQSLILSLLLIALFVLFSLAP
                     QQIGLYKDVNSVSYSYKQLIQKHDTLLDDLGKNSLKPFVSGHLGSADLSKQYYHLRNH
                     LQSQTELLVFSPNQELLFASNSHLGNFFSKSIYISEVLDKAKINQRLLKIIVDSEGGH
                     YLALIKPIIVNKKVSGYAFLLMNGKDFLLPTKAINSDLIIADQLNNSFTFTNRDFISS
                     SLDKVDSQFLTRYFSFHDHRAFVVRKVALQDNILLYMYRPLIPVTLVVLFSLVSSVII
                     FVILRQKSRVLADRIAVKNSSAINQMVLDMDAISRQEKSSIELDSQDEFQYLSVQINQ
                     MVSQLKDLHEKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSQLTERIV
                     IQLTKLLRYSLSGSTEAAMLKDDLAIIESYLLINQVRFEELTYTISVSPELEHMRVPK
                     LFLLPLIENAIKYGLKERHDVAINIDIWQDSDGIWFTVSNNGSGISLARQQAIRTMLR
                     STHSHHGLINSYRRLQYQFSTVLLEFTKTDDAFRVSYIVKE"
     gene            complement(26379..27206)
                     /locus_tag="VBE22_000698"
     CDS             complement(26379..27206)
                     /locus_tag="VBE22_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO7202171.1"
                     /translation="MKSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASTGSAMGAVVFLLLYNIPAFLIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQSYLDQLFVGVVPLLVTILAFWLLRKKVNINWIMFGIMVLGIVLGLLGIC"
     gene            complement(27203..28012)
                     /locus_tag="VBE22_000699"
     CDS             complement(27203..28012)
                     /locus_tag="VBE22_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO7202172.1"
                     /translation="MIMPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGMLLGDI
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISNIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNSFVHHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDIELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            complement(28028..28519)
                     /locus_tag="VBE22_000700"
     CDS             complement(28028..28519)
                     /locus_tag="VBE22_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7202173.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEESIALFKDPRATDKRIFVIVNSVKDACTIAKNITDLEAVN
                     VANVGRFDKSDPATKVKLTSSLLLNTEELEAAKELASLPDLDVFNQVLPSNTKVNLSQ
                     LVK"
     gene            complement(28536..28961)
                     /locus_tag="VBE22_000701"
     CDS             complement(28536..28961)
                     /locus_tag="VBE22_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEO7202174.1"
                     /translation="MKRKFLIGSHGKLASGLQSSIDILTGKGQEIQTIDAYIDDSDYT
                     KSIVEFIDEIAPDEQGLIFTDLLGGSVNQKMATAVMNSGKNNIFLITNSNLATLLSLL
                     FLKPEEELTKEEIVTVINESQVQLVDLSFKAGSEDDFFD"
     gene            complement(29208..30242)
                     /locus_tag="VBE22_000702"
     CDS             complement(29208..30242)
                     /locus_tag="VBE22_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO7202175.1"
                     /translation="MDNKQHNTSMISLLQYLFSILVILVHSGRLFSQDVIHFTFKSFL
                     GRMAVPYFLICTAFFLRGRIQQGLCNHSYFRKLIKKYSMWTIIYLPYGYFFFESLNIA
                     KIYLLPGFIVAFLYLGMSHTLWYIPAVILGWVIIQGLLKYVGTRGTFITVVVLYCIGA
                     VETYSVFIQSTKFYPLMSTYMSIFQTTRNGLFYTPVYLLAGYLLYDYFNTDLFTKSRG
                     LKYILFLLLLALENVLIYFNQGLDKNFFLLAPLCAVFLFNWSIRTSLFKEYRLSPLKQ
                     LSVYYFFLPPLFIGIVSYCLKSTSLVAHHQGKVIFVVTLALTHACALLIIKVVNKIPY
                     QKIIPLSLSK"
     gene            complement(30471..31625)
                     /locus_tag="VBE22_000703"
     CDS             complement(30471..31625)
                     /locus_tag="VBE22_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEO7202176.1"
                     /translation="MISIFPFELKKLLQRKAAFGAFIIFLLAIFGLFYQHFFVGQISG
                     YSADKIHGREAVAINRRIAEKYSGDLSEERVTNIIKNYVNPPAGRPPIFDVFSYYVAD
                     TFDRDFLIYYNQLKDDKTIDFNKINIKDQKQLGSALPLKQLKLGNFAPWNQLFEVSNS
                     TYILIVLFTLFLSAPLFSGDRAKKINPILLTTRYGRGQLTWAKLASTMTIGISTFILA
                     YALILTVFADYFGFSGWETSVQLNLYWTSPLANIMGFPVPMTISTAFVHIIVLQFVGL
                     LFIASLNFFISSITNSPLTSFAVSTLAFFAPSFLMAMFNKGVINKLLTIFSVSTTDTS
                     ASLLKLSRNGSHGFFFDSFSANEICLISLRMLIAVLLIAYTYRLMRRRSL"
     gene            complement(31631..32479)
                     /locus_tag="VBE22_000704"
     CDS             complement(31631..32479)
                     /locus_tag="VBE22_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202177.1"
                     /translation="MLLQLDNITKSYGKKIVLNQISYQFTPGLYGLLGANGTGKTTLL
                     NLMSHFTFADSGNIYWNGQEQSEEFYRHIGFLPQHFRYYDQFTGIAFLNYIATLKGVD
                     KKKAKQEIPRLLELVGLGDVGKKKISSYSGGMKQRLGIAQALINDPEILILDEPTVGL
                     DPKERVKFRHILSQLSTNKIIILSTHIVSDVEAVAKEIIVLKNGKFIEHGNTAQLLKT
                     IEGKVWEITTEPGLSQIPNIAIVNEKVFSDSRVFRVVSDICPSDSAQLVVPTLEDFYI
                     YHFRGE"
     gene            complement(32602..32901)
                     /locus_tag="VBE22_000705"
     CDS             complement(32602..32901)
                     /locus_tag="VBE22_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO7202178.1"
                     /translation="MANMIDYSHHIEVSLETDEPKIAMSYFTLNHKQIFIIENSIVEE
                     RIDASRLFGYGASSKGEDRGLGLYNVMKVIDRYPNASISTNSEQHTFRQGLEIIF"
     gene            complement(32970..33722)
                     /locus_tag="VBE22_000706"
     CDS             complement(32970..33722)
                     /locus_tag="VBE22_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7202179.1"
                     /translation="MNIFILEDDFVQQAHFEKIIKEIRVQYNLHFKTVETFAKPVQLL
                     ESIYEIGLHNLFFLDIEIKNDEQMGLEVAKQIRQVDPYAQIVFVTTHSELMPLTFRYQ
                     VSALDYIDKGLSQEEFSQRIEEVLLYVDGICNKPLVENSFYFKSRYSQVQLPFNDLLY
                     IETSSRSHRVVLYTEKDRMEFTATLGDILKQEPRLFQCHRSFLVNPLNIFKVDRIDRL
                     VYFQNGTTCLVSRNKVRDIVSIVDKYQKDRKR"
BASE COUNT        10885 a         6503 c         5357 g        11017 t
ORIGIN      
        1 tgcaaactaa ctaaatttta ggtatccatc gaaatcaaat aggcataggc taaatcatct
       61 aatattttta aattaagtcc cgaagattgg gtaaatttat ctaatttata ttgcaatgaa
      121 ttccgatgaa tgaaaagtgc cttagccgtt ttagcaagat ttccttgctc ttgccacagg
      181 gcttttatta tagccgaagt atcactcgta tcaaggatat gttgtaaaca ttttgcttta
      241 atagaaggca tgggcgattg atttgccaaa gcccataaag ccatctttgg aaatcgacag
      301 taatgttgtt cggccttgtg actaagaaaa agcgtaaaaa gtttatattc ttcttcatat
      361 aattctttcc agtccactgc ttgtagatgc gtccaactat ttccaaaata gcaccttaaa
      421 cgcgtgttaa agtcattttc aatcgttggt aaaatatctg ttagtaattc cgtagcatta
      481 aagaaattat cctgatttaa aatcatgaca ctttgggttt gattaattgt cataattgtt
      541 tctacgttta ttactaaagg cgacaagaaa tccttaagtt cttctgataa ggtaaattga
      601 tgctcaataa aaataaattg gactgccgaa tgagattggg gcaaccttcc acgaccttca
      661 actagatagc ggtaccaagg attctgagaa tccactgaac tcgcatcttc caggcttggt
      721 gtcatttgta ataagtatag ttcacgctta gttagcgaag attttggaaa gtggaggaca
      781 tgaccatctt gctcaacgat gatataatcc tcactctgtt taacttcctg tttagtaata
      841 acttctggaa acagttcctt aaaatccatg gcatcctcca ctacagtttt gttcattata
      901 tcatatttat aacctctttt tgagcacaga aaaagaggag aatgtctcct cagaaaattt
      961 ctgttataaa atagtgatcc gaatagaatg ctgtttcgac tgctttggat taagccgtac
     1021 gatattttgt ttatcttcta aaatatcaga ctcttcaata gaagttgaca aagaactcca
     1081 gggttccaaa gccaaaaaag gaccaccatt gttacttgtc caaagaataa gattctcaaa
     1141 atcatcaaag tcaagttgga ttccttttgc atgatttcta gattttaagt aaaccgtctt
     1201 agatcgtagt tggtctaaag taatagcatc tttttcaaat aaagcatgat taagagataa
     1261 ttgtttttga ttttctaaaa aaggatgtct agcttgtaaa tctaacaagc ctgtatccgg
     1321 aaacgactca ggaattgtgc aagtttcaaa ctgttcaaac tctagatagt aatcactaaa
     1381 tacttccttt tcaaacaaag ggcaattaaa gcctgggtga gctccgatag catatggcat
     1441 atttttttct gattctaagt ttttgacaat atatgtaatg gcaatgcttt tatttcttaa
     1501 ggtgtaagtt atttctaaac taaaacgata aggataattt atcaacattt ctttactact
     1561 tttaattgta aaacgaacag aagaatccga tatgtaatca tagtcgaatg ttttgtgacg
     1621 tacaaagcca tgccttggta tgatacccgt aaaatgtgga gcttgagctg gtctgtaaat
     1681 gacgcaatca tttcgaacac ttccgcatat aggaaataaa ataggagctt gcccacccca
     1741 ataagtggcg tccccttgcc aaaggtactc aacgccttga ctatctgtaa tagaagtcat
     1801 tgttgcacct aacgctttta atgtcacttg caactcatga ttttgtatcg taatagccat
     1861 tatttttcct ttcataaaga acaataattg ctttaccagc ttttctctag caattatctt
     1921 tccttccaag aagttgctgt tttttgcaaa attttattta gttcatcaat attttgaaaa
     1981 cctattgtac gtaaccattg acgtgcacca gcttctcctt tttcaacata ctctttaaca
     2041 cttccagccc aagttgctct accacaaagg acaccgttaa atttagcacc agcttcttta
     2101 gcaaacacaa gagtctcttg aaaaagctga gcaggaactc ctgcactcaa aaaaatgtag
     2161 gggagtagtg tcgcctgatc ttgttctcta aaatatgccg cagctgtcgc tttattataa
     2221 gcagtctctc cttgcgcaaa accttctaca tagtccatat tgacaggcac ttccaccttc
     2281 aagacatcaa tattaaatcg aggatttgaa aaaactttca tagcttcaat aaccttacgt
     2341 ggcttaattt tggcatactc aatacctgag ctatccgtaa tcttttcatc atatgacaaa
     2401 atttcaagga aaaaaggaat gtcttctgca acacattcag aaccaatccg ttcaatataa
     2461 gcttcttttt cttcgtttac ttcatccgaa ctatcaacat cataataaag taagaactta
     2521 actgcgtctg ctccctcttc tttaatgcgt ttcgcagacc aaatgtctaa acagtctggg
     2581 agtcgcttgg tactactggt atcataaccc gttttttcgt acgcaagtaa aagaccggca
     2641 ttgtcatcta aaactttcgt agctggcaac ccatattctg gatctaacaa cattgatgaa
     2701 gcgtaagggg ttaattcttc agctaccaaa actttaagtg cttctatttg agaaacagtg
     2761 ggctcctcag attgatattt tgccattaat cgcttcaatg ctccacgttg gtcaaaagcc
     2821 aaggccgaaa taataccatt tttatcactc aattgttcca tatgtttttg tttttgttct
     2881 gttaatccca tcctatacct ctttcacttg gatttgactg attaattctt gatagtaggt
     2941 cagattgata tgccctgtta atgtttcttg tgcattaagc atacccaaga cattagctct
     3001 cttaagcaaa tttgtatcat ctagttgatg ttccaaagca gacgcgattc ctgcaaccgt
     3061 tgcatcccct gaaccaactg gattaataac ttctattttt ggaatcgtca cttgatagta
     3121 gtgattccca tgtttagcaa aagctcccct agcacctaaa gaaacaataa cccaatcaat
     3181 tcctctaaaa agatcttgct gtaaggtaga ttttaattcg tctaaactat agctaattgg
     3241 ttttccaatt aattgtgtta attcttcaag gttaggttta atgacggtcg gcttagcact
     3301 agattttaaa accgaacgta atgcttctcc tgaacagtct aaaacaactt tcttattaaa
     3361 gtgattcgct agggagatta acctagcata ataatctgag gggagtccct ttggtaaact
     3421 tcctgatact gttatgatag ttgcagcatc aaagataagt tttagatgat tcaaaaaggc
     3481 gtctgcttca tctttagtaa tatgtggacc tttttctaga atttcagtct gctgaccctc
     3541 atgtaaaact gcaatacaat gacgtgtttc tccaccaata cttaaaaatt ggtgtctgat
     3601 ttccaatgca tctaagccac ttcggatgaa atctccaaaa tcccccccta atagaccagt
     3661 tgccactaca ttatcgccaa gttgagaaag aacacgagtc acatttaacc ccttaccccc
     3721 aggtgttttg gagacgtcgg taactcgatt tacagtatcc atgttaaagt tttctaagca
     3781 gtacgaaata tcaatagaag ggttcaaagt tactgttaaa atcatagata tctccttagt
     3841 cgtgatattc accacgattc catttatcta agaactctgt gaaaaagtca ttctcttctt
     3901 gatgggcatt atgagtctca acctcggcta tcttttcaat taatactttg ttttcttttg
     3961 ttggtttata tttagctctt ataaacgcct cgatgatatc tgtcattaac agcccaccag
     4021 taattttccc accaaatccg ataacgttag catttaattc ttctttagca tagatagctg
     4081 atgtcaaatc acgtaccaga gctgagcgaa ttcctggaac tttattaaca gcattattaa
     4141 taccaactcc agttccacaa atacatacac ctaagtcggc ttttccacta gcgacagctt
     4201 ctcctacttt cttaccataa ataggatagt gagtacggat attatcataa gttccacaat
     4261 caataacctc ataccctttt gtttttaaat aatccacaac tgcaattttg tcataagtta
     4321 caatatgatc acaaccgacc gcaattttca tctgctttac ctctcttacg cttagcacat
     4381 tttattcaac atatcgaccc gaatttgatg acgacctgca tcgtaagtcc catcaacaaa
     4441 accctctact atatgtttag caactcctgg accaacaatc tctgaaccta aagtaatcat
     4501 cctagcgtta ttatgagctc tagtcatata agctgagcgt tcatctgaaa cttctgctgc
     4561 aatcatcccc ttaaccttag tggctaccat aaaaggacca acaccgtaag catcaaccat
     4621 tattcctaag ttatcttctt tttgattcac tttagcaacg attgctaagg tattatcaac
     4681 gaaatctttg ctagtatccg ttaaatcaat aatttcctta cctaaacttg taaggtgttg
     4741 tctaatcact tccttaagtt caacaccatg cgcatccgcc ccaataataa ttgtcatttc
     4801 tttctcctta tttcactttt aatacaattg tatgtagttg gaagatattg tttgtttttg
     4861 tttgtaatat catcatatat cttatttttg tttttgtcaa tgttttgttt tgtttttgtt
     4921 tgaaatgaac aaaaaaaact ccgtgataac tatgtcaact aaaattcact actagctagc
     4981 tatcacgaag tttgatactt cttattattt ttatttgtag gaacgcttat tccgcaagcc
     5041 ataatcataa ataaaattta aacaaaacat gccaatttgc tgccattttc tcccataaag
     5101 tgccttgctt ctctgtattt tgactaagaa aactccaatt aaaaactttg taatttaaag
     5161 taaaactatt ttgatgtttt tctctatgtt cataaaggac accaaattta tcattattaa
     5221 gttgttgaac agagttgtaa gcaaacgaac catcttgaat gtgatggtga tataaccact
     5281 taaaactacc atttcgatta acttgcgcta gacgtgcata tccatcttga cgcttcttac
     5341 ctggcccatt agcattcact aataaaatat actctttttt gtcatgctca aagcgaatag
     5401 ctgataattg gacgtaagca tcatgaactt ccttatatcg tttaacatgg ttttgccaag
     5461 tctccccgcc gtctttactt gtggctactt ctaggttacc agttagattc ctcataaata
     5521 acttaatgtc cccatttttc aattgaacgg gtacggattc tgtattttgt tcttttttat
     5581 tatccattgt taaggagtga attttttcac cattagaaag tatacggtta tcattgactg
     5641 cttttccagt atgccacgtt tttccatggt cgtctgaata aattaggcgt gaagattgtg
     5701 aacctcttag atgagatttc caattcgtag aataggcagg aataataata cgaccagcgt
     5761 gtggccccca ttttaaaact ataccttttc caggtcctat tcctaaaaat ttcatgcctt
     5821 tctgacgaag acttgctgtt atatctctag gtgatgacca tgtcctacca tcatcatcgc
     5881 tatatgacat ccacacatag cttgattttg ctaaacgaaa tggtgacgtt ttatgttttg
     5941 taaaatatat atttccaagt agctgttttc ccttataaat atcccctaga tttgaatggt
     6001 cacttttagt agtttctgtt ataacatgat aatcagtctt tttcccttta aaattatata
     6061 caataccttt gtcacggata gtaaaaacct tcgatttctt tccattatta tataaattaa
     6121 gataagactg tcctccgatt tgggtatatt ctttttcagg tgtgttagca atactaataa
     6181 cgcctcttcc ttctggaaac atatcatata ttgaaaaaat acgtttcgtc ttagagctag
     6241 tatcttgaac taatgccata tccatattaa taggtgagcc actatagtca ccactagtaa
     6301 ctagcggaac tctagggtta ttacggagat tgacaatagt ttctcttttt ccccaagtga
     6361 caccatcatc ctcactacgg cgaataacca ttcctatgtt tccccaatca caagcttgct
     6421 caattctttc atcagctccc acaagtaaag ttcctttttc cgtttttaat aatgagggga
     6481 tacggtaacc aaacacatta tctttatttt ttctcccaaa atctcctccc ctatatactt
     6541 ctgttttagc agaaagtata gcacctttag gtaatttgag ctgatggttt agatgacaat
     6601 aatattgaaa ataagatttt atatcttcaa aattcaaatt atcaacacag caaacaaggc
     6661 agtgtgcctt agcttttaat ttttgccata aattaccact atattttatt tccttctcta
     6721 ctttctcttt gccaatagca agcatcccct ctataacaga cgataacttt ccatcctttt
     6781 gagagaatgt tttagtaaga tggtgttcct gtatcgcatt ctttttaaag cccaacgata
     6841 tctgactagc aatgtccgta ttacctatga cattacttgt cgataaatct cccttaggaa
     6901 tgatatccaa agacctcaac ttatcccttt caaaacctct atcctctctc gctgtctttc
     6961 taaattcatc gctattaagt aatgcaaccg aataaccctt tgattctatc aggaatctct
     7021 gttgttctgc aatggaatga ctgctttcgg gtataatata ggtagttcta ttattaggtt
     7081 gagttgcatt tagttctgct aaatttgcct gtttacgatt ttcttctgtc gctatttccg
     7141 attttagagg aggtgacaat ttttcttgac tgtcagaatc acctaagact attaccatat
     7201 ctttatcttt ttgatcatct actattaaac tgtccgatgt atcagaatct aattctaact
     7261 gcctctcctc tattgaatta gtaagcttat ctaaagtaga gtcaaatatg ggaacagtag
     7321 ttttttcatc atctattgat ataaaagagt ttatatcaga ttttatttcc tcactataaa
     7381 ttgtactagg atgcaaaaat ccacataata agataaaacc tagcatcata tatgtactag
     7441 ttatcactcc tagacggcgt atcgttatag aaggtttccc aaaggttaat gtcatagaaa
     7501 ctccaagtct taaaatattg gttgcataca tcaactctag aaaaatggga gttcagtaat
     7561 gtttccaaaa aggattgtat accaacttat cactattttc aatatatttt tataatttta
     7621 ctagtttttt attattaaaa tgagcgaaaa ttaattatat atgttatttt taaaatcttt
     7681 aatatatcaa aaacctagaa aatataacat tctcctaggt ttttttattc tatttatttt
     7741 tgattagctg catattctgc attacggcgt ttcatttgtt tagcatacca cataaacaaa
     7801 ccaagataag ctgtcgcaaa aataattaat gttataatcg ctgataattg acctgttgtc
     7861 gctttaccaa taacgtaacc aaagattttc tccatagggc cttctagtgt agagtgagtg
     7921 ataagtgaac ctgctcgcgc cccagctggg aatgcgccta cctttttagc taattcagtt
     7981 gcaaatgggg caatcattgt tcctgataaa agaaatagcg gtaatagcaa tgttccaaag
     8041 gtaatcatac gaattaaacg gccacgtgta acaaccagta aagcaggggt tacgcccatt
     8101 gcgataatac cagcaagcgg tagaatacca ttaccaactt tagagagtaa aatagcttct
     8161 actagcataa tcggtgcaag aatgttagca catgcccaaa tctctgcacg tccggcaata
     8221 aatggccagt ccaaaccaat attgaaacga cgaccatgca ttttaccatt agcaaaatta
     8281 gtaattcctt gagaaagtgg ttctactgca gcaatgaacc aagaaccaat caaagagaac
     8341 aactctaaac aagcacctgc tgtaaaaccg agagaaaacc agccacctaa tgctgcaaat
     8401 gagaacacgg ggttgcccat aatccctatg acaagtccta aaatcatacc aataaagaac
     8461 ttagagcccc agaatccaat ggctttattt aatttagccg cgtcaaagtc atatttatct
     8521 agccctggaa aaactttatc aaaaagctta tccaaaacca taataatggg gttcatcata
     8581 taattcatat gcgttgaagt catgggagaa gttggacctg tccccaataa atcatcaaag
     8641 gtagttttca tcaaatcaga attgataatt tttagaacac caacgaagat aatagctaca
     8701 gttgcaataa tgacagataa aagtgttggt agattatttt ttttagcata ccacataatt
     8761 aaaagacctg tgatagataa atgccaaata tcaaaaatat caacatctaa ggtatccgtt
     8821 tttttcataa caatcataac aatattgaca ataagcatga ttaataagaa gtaaagcgta
     8881 tatggtgatc cccatgtaat agttgctaat ggagcccaac caacgtcaat aatgcttaga
     8941 ttgattcctg tatttttaac aaaagcttga agtgattcag aaaaagcacc agttaaaata
     9001 ccaataatag caccaatacc cgtaagagca atagctaact taataccacc ttcaagtgct
     9061 ttagaaaact tcactccaaa tatcatggct aaacctgtca tgacaattag catcacaact
     9121 cccgaaccca tatggataat tggatcaaag aatttattga caatatttaa aaatacctgc
     9181 atgttccttc tcctttaaga ctaattccaa ttttaaatat gttatgatag attgtgttct
     9241 ttgataactt gttctaaagc agtaaagacc ggttcgctca taactggtat gcgataaaga
     9301 ataggtcctg cttctacaac tggaattggt acattaaaac ctaaatcagt tttagcaata
     9361 ggagtaaaga tatcatattt gttgaccatt tcctcagtga tatccttaac cataacagca
     9421 tcacaatgta cattgtaacc tctatctcct aattgtgtct cgatagcatc tttaatttga
     9481 tgacttgagt ttacgcctgc accacaagct gctaatattt tgatcatgtt aaaacactcc
     9541 tttttctatt aaataatgat taattttttc tttattgtcg ccaagctcat gtagccgatc
     9601 taggtttcct tttgtaataa agaaggtaat caaattagat aaaatatcac tttggttact
     9661 attcctacta ttaatgatga agaaaaaatc ctgaacccta cactcctctt ctggattaat
     9721 catatgtttg aaagtaattg gctgactatt tttgacataa aagattctat ctactagaca
     9781 atactgcgtt tcagtgtgtg gaatagcaac atattgaata ggagtcccat cttttaaatc
     9841 aatttttaac cccgttggaa actctctctc cctttctttt actgcctgtc ggtagttagg
     9901 actaacatag tgttgtttta tcaatgcttt tgatactaag tcaaataatt cttcctgaga
     9961 atgggcatca ataaataaaa tatcttgagt cacagcaaac acctttcttc ttagttaagc
    10021 ggttacattt ttgttgtaag gcaaacaagc cttatcaact tatctacatc aatattatac
    10081 aaaacaaaat aaaacattac aagacatttt ttattttatt ttgtttatta ttgtttattt
    10141 tttcaaaatt attattattt tagctctatt tatggtatga tatatataac gaagaggtag
    10201 gagaggtcat tatgttaaag cgagaaagat tacaaaaaat aatagaaaaa gtcaatataa
    10261 atggcattgt cactgttaat gaaatcatgg aagaacttga tgtatcagac atgactgtta
    10321 ggcgtgactt ggatgaatta gataaagcag gactgttaat acgcatccat ggtggtgcac
    10381 aaaaagtaaa tgcatcacca actccacaaa attatgaaaa atcaaacact gaaaaatacg
    10441 atattcaaac aaatgaaaaa ttagaaattg cccaatttgc aaaacaattc atcaatgatg
    10501 gtgaaacaat ctttattggg cctggaacaa ccttagagaa attagccacg caattacttg
    10561 attttaaaat tagggtagta accaatagcc ttcctgtttt caatattctg aatcaaagtt
    10621 caacacttga ccttatcctt gtagggggag agtacagaga aattactggt gcttttgtcg
    10681 gttctgttac tattaactct attaaatcac ttaatttttc aaaagctttc gttagtagta
    10741 atggtgtttt tgaaaagtct atcgctactt acgacgaggg agaaggtgaa attcagcgca
    10801 ttgctcttaa taattccttt gaaaaattcc ttttagttga tagccaaaaa tttggtaaat
    10861 atgattttta tactttttat caattagacg atattgattt tgtattaaca gatcataata
    10921 ttgataatgt tgttaaggag caatacagta gcttcaccaa aattttaact aataacaatt
    10981 acatgtaata aaacaagtga gccactacta gaaacctgtt atacagcttt tagtagtggc
    11041 tctttattta gtgattataa ctttagtcgt tttataaaag tcaaaacaag aaaacttcct
    11101 caaacaaaaa tatattaact ctctttttaa gcgtaaaaca atggttgttc aaaagaagaa
    11161 ttagatatat cattaaaagc ttcgcacgct agaaatcata actaaaaaac aaacaagtac
    11221 ggtgacttgt ttgtttttag ttgtcagcta agtttactca actcttttat tatttcactt
    11281 ctgttagtgt tttaatatca acaagcactt tagagccatc ttttttagta acttgaactt
    11341 gactaccatt aatataatta aagctttcaa aacttacact atatttattt gataattgga
    11401 tgagttgctt ttcaaaagat tgtctatcca taccatattt cttagcgttt tgtgccattc
    11461 ctagttcata ttcatctagc tctgattcat cttcttgttt ttctggttct tcttcgtctg
    11521 aacctttatt aacatcataa aaaccatagt tgtgagttac tttccctgaa tcttcaggtt
    11581 cttcatctgg agcataattt ttggctttat tgtctttatt tgagcctttg ttatgatcac
    11641 tgtcgtgtcc ccaacctttt tcttttggta attcattagg atgttccatg tagtatttaa
    11701 ttgttgagaa gagttgttgt aatgtatagc cttctggtgc tttccataaa ccacccttgt
    11761 caaaccatgc cataggaaca ttatgataat gatcatgatg aggaacaatc aattgatcat
    11821 cttcaaatcc aactgcatat gccatgtgag cagcaatttt atcaagcggt acaatttttt
    11881 ctggttttgc ttgtttgaat acttctgcag cacttaagtt tttgtcacta gaatttgatg
    11941 ttgatttatt atcagttggt gcaggacttg gtttatctgc tttattagag tccttttcct
    12001 tataaatttc agcatcttta ccccatcctt caactttagg acgtttttct gggtgcatca
    12061 tgtaatattt agccgtagct aaatagtctt ctaaagaata tgtcttatct gaatcagcta
    12121 aaagatcttt ttcttcatca aaccatttta attccacata atggtaatga tctttatgag
    12181 gaattacaaa tgtaccattc ttatagtctg tcgcttgtgc aattccaaat aataaatctt
    12241 cagatttaac aattgctttt ggttcaattg cctcatagat ttcttgagct gttttacctg
    12301 tttttggttt gtcttgattt gcttcagatt ttcctgaaag aaattcttga gctgctttta
    12361 attccgactc tgataaatct ttctttggta cccaatgctc atgtgtcata tgtggaatga
    12421 cataacctgt acctgtatct tttttaatat ctttggcatc aaaaatgtaa ccatcagatg
    12481 ttgtgtattt accagctttt ttgcctcttc aatttcttga gcactgtaga caatctggct
    12541 atttggtttg cctttacgtt ggtctttcgg tgttactgca tggtgttctt cctctgtgtg
    12601 aggttcctta ttaggttcat gagaaatatg ctttggtttc tctgtagaat cttttttccc
    12661 taatgcatca atatgatgtc ctctatgctc tgccaccatc cttgttgctt ctaactctaa
    12721 tggagacata tccttatagt gaataaagtg gaaatgatta ccgtgcggaa tagaaacacc
    12781 gattgctgtg aaagcattaa cttgtgctgg gtcataaacc aaaccatcac tttctacgtg
    12841 acgagcatat aacggttgag catataaacg tttcaacatt ttgtctaaag cagaatcttt
    12901 gttgattggt ttggttgtag gtgcatggtg ttttggtgcc gtagcatgat taccagctgg
    12961 ttttgaccag tgatttgcgt gagtgactct tggaatagaa ataggggcat tagcatgtct
    13021 tcctgttgat acattaccgc ttggtgcact tgcatgaatg tatgaacttg aatttgatgg
    13081 ttttgagcta ttacctgatc ggccagtctt gcggttccaa tatgcctgtg cggctgataa
    13141 ctctgatgga gataaatcag ctttcgggat ataatggaaa tgatcgccat gtggtactaa
    13201 aaaggcatct cccatgtcat caatgacatc tgttggacta aaaacataac catcatctgt
    13261 agtatagcga ccgctagctt tcgcttcacg tacgctggct gataaagctt gagtttttgc
    13321 actactatgc tttcctgatt ctcccttaga accaccctta gaccattctt cgcgttgctt
    13381 ttggatttgt tcctttgtac gaacatttgt tcgtttgcta ccttctttaa gatagaggta
    13441 atacttaccg ttaactttga taatataacc atctttgact tcattaatga catcagcttt
    13501 attaaagaca tagctagggt ctttcataat caattcttca ctaatgatgg catcatatgg
    13561 cactttccca ttgtaataat ggtaatggtc tccatgagaa gtcacataac cgtcgtcagt
    13621 aattttaaca acgatttgtt ctgcagatat gccttcttct ttactgattt ggtcgggcgt
    13681 tttatttaca gctgttgctt gtgctttgct atcagattta tctgttttca cataagaaac
    13741 actattgtct gatttttggg cattatagct acctaattgg taagcaccta caccagtacc
    13801 aaatagagct actgataaag cactaataat aactgttttt ttacgattca tcctgcgtcc
    13861 tttctttata taactaccta ttgatttaac gatttgacaa gtaccttaag attagtttcc
    13921 aaattttcta agtaatcttt attgtttttg ggaactgctt ctaaaggact taaacttgca
    13981 attttaactc gagtagctga agctactgct tgagctaatt taggtgagac tccttcttca
    14041 acaaaaatag tcttaacctt atatgttttc acaaactcct gaatttcggc taatttttta
    14101 gcactaggtt cttgctcggt tgagacacct gcaataccta actgagtcaa tccgtatcgc
    14161 ttagctaagt atgagaatgc tgtatgtgaa gtcacaaagt atttagactt tgcagcttta
    14221 aattttggct tatacttctc tgcaatagcc attgccttgt cactaaattg atcagcattt
    14281 ttttgataaa ccttagcgtt tttaggatcc tttttagcta attgtgtagc gatgagttgt
    14341 gcttcctcag atacttttac agggtcattc caagtgtgag ggtcatacaa ggttgactca
    14401 tctactcctt tttctgcctc tacatcttct aagccatgaa ctttatccaa agtcatacct
    14461 tttgaagctt caattacaga tactttagag tgatgcaaac taggttccaa acgtctcgcc
    14521 caagcttcta gtgtgtgcga atgataaaga aatagatcag catcataaat ggcagcaaca
    14581 tcacttgatg agggttcaaa accatgaata cctgactgtg atcgaatcat tttaatatca
    14641 ttcaaatcac cagaaactgc ttttgtaatg gaatatactg gataaaagct agtcactact
    14701 gacaagccat ttttttgctg gctttgttta gtacaagcac ataagcaaat tagggtaact
    14761 actccgagaa caaaaataag gcttttccgt atccttttca ttaaacctcc tttaaaataa
    14821 aaaatactta accagttaac attttagagt attcttattt tagtgtcaac gaaaaaccat
    14881 aaatctaata tttttctatt tttaatattg ttaagtgcac ttaatttcat atcaacaacc
    14941 tttattcatg cctttccaag ctacagtaaa tcttttcata aacatgaatg ggttaaggag
    15001 tataattaga accacaataa aaagagccaa gcattctgcc caactctttg gttttttatt
    15061 tatttttagt atctaaaacg atagttactg gtccatcatt aatcagactg acttgcatat
    15121 cagctccaaa ttgacctgtt tctaccgaaa catgatttgc taattcttga ttaaagatat
    15181 cataaaactg attcgcttta acaggatcag ctgccccagt aaaagctgga cgatttcctt
    15241 ttttggtatc tgcaaagagt gtaaattgtg agatagataa gacgcttcct ttaatgtctt
    15301 gaattgaaag gttcattttc ccctcatcat cagaaaaaat acgcatgcta gtaattttac
    15361 gaactgcata agcaatatct tctgttgtat cttctggagc aatcccaact agaagtacta
    15421 aacccttctc aatagatccg actacatcat cttcaataga aacacttgct tgattaacac
    15481 gttgaataat gattttcatt ttgctctcct aaccattcgt acgtttaaca gaataaacat
    15541 ctgggataat tttgatttta tcaaccacag ttgtcaattg tgctaaattt gaaattccaa
    15601 agctaacatg aatattagca aatttcatat cttttgttgg ttgtgcattt actgtcgata
    15661 ctagctttgt tgcatttgat aaggtttgaa gaacatcatt aaggaggcca ctacgattca
    15721 aaccataaat atcgatttca gccatgtact cttttttaga tccatcatca tcccactcaa
    15781 catcaatcaa acgttgttcg tagttctcct gactctttaa attttgacaa tctgaacgat
    15841 ggatagccac gcctctacct ttagttatat agccttcaat gagatctcca ggaacgggat
    15901 tacaacattt tgcaatacgc attaataaac ctgaagctcc ttgaatgata acaccatttt
    15961 cactcttaac tttgagaaca tcacgcttat cggttttgat ttcaccaccg ttaataagtt
    16021 cgtctgcttc cgctttagcc ttagcacgtt cctcctcacg gcgttctttt tccgtcagtt
    16081 tattaaaaat actaatcgga ctcaaatcac caaaacctac ggctgcatac aaagcttcct
    16141 cacttttaac actgacacgt ggaaggattt cttcaatatg tttcttatct aaatatttgt
    16201 taggcacata accttgttct tggaaataat caaccagtaa ttctctacct ttattaatag
    16261 aagtttcttt atcttggttt ttgaagaact gacgaatttt attgcgagct ttattggtct
    16321 taacaatctt aatccagtca cgacttggtc caaatgaatt agggtttgtg ataatctcaa
    16381 caacatctcc tgtttttaac ttagcagtca atggaaccat acgtccattt actttagcgc
    16441 cagtagcttt ttctccaact tgcgtgtgaa tagcataagc aaaatcaatt ggtccagatt
    16501 cccttggcaa ttcctgaaca gcaccatttg gggtaaaaac atagatgcgt tctgtaaaga
    16561 tatcttcctt aacagagtca acaaaatcct tggcgtcttt tgattcatct tgaagctcaa
    16621 ctaattcttg aatccatccc ataccaaccg attgctctgc ttgattaacc ttactggtga
    16681 ttcctttttt ataggcccaa tgggctgcaa caccaaactc ggcaacttga tgcatttctt
    16741 tagttcgaat ctgaatttca attggccctt taggtccata aaccgtcgtg tgaatagatt
    16801 gataaccatt ggcctttggt gccgcaatat aatctttaaa ccttcctggc ataggacgcc
    16861 ataactcatg gatatacccc accatggcat aaacatcact agcagtttcc atgatacagc
    16921 gtatcgcaat taaatcataa atctgatcaa agcgtttctt tttatcacgc atttttctgt
    16981 aaatagaata aatgtgtttt ggtctaccat aaatatcacc atataaaccc tgttcctcag
    17041 tataggatct gattttatcg acaattatat caaccaactc ttcgcgttca cgacgttttt
    17101 cactcatcat atgagaaatc ttataaaatt ccgtctcatt caagtaacga aaagataaat
    17161 cttctaattc ccacttgata cgactaatcc ccaaacgatg cgctaaagga gcgtatattt
    17221 ccatagtctc acgtgaaata cgctcttgtt tatcttttct gaggtgttta agggttctca
    17281 tattatgtaa gcggtcagcg agcttaacca aaataacacg gatatcctta gacatagcca
    17341 ttaacatttt acggtgattt tctgctaact gttcttcatg agatttgtac tccactttac
    17401 ccaattttgt tacgccgtca ataatatcgc gaacatcctt gccaaaatca gtttcaatct
    17461 catcaagggt aatttccgta tcttctacaa catcatgtaa aaaaccacaa gctaccgtaa
    17521 cggcatcaag gtgtaaatct gcaagaatac ctgctacttg aattggatga ataatgtagg
    17581 gctcgcccga ctttcttgct tgataataat gagctgcggt tgcgtaattt aaggcaaatt
    17641 ttacaaaagc aacatccgtc tcactcatgt actgggatgt gattgcaaca acctcttctc
    17701 ctgttaaatt aatttctttg accatagtta tcctctttaa tcatagtgat agattgtacc
    17761 tattttatca ctttttaacc taaaaataaa agaagttaga ggctcaaaaa gaattaaata
    17821 ccaagtaaat gtgtctaact aagttatcct ttataaataa acgattgttt ttggtgtaat
    17881 cactaaaaat aattcgtaaa tgagattgat taaacgtttt caataagcta taatagtttt
    17941 tagattagga taatatacat gtaatgtttt aatggaggag atttatgtca aaacattatt
    18001 tttcaaaaag cgtattcgct ttaactgtct taacagctac agcaacaagt ggtttagctg
    18061 ttcaagcaga agacattgtt acaacaccaa gttcaacctc tacaaaggtt gaatcaacta
    18121 cccccactag cacaattgca gaggaaaagt caaacgttac ttctactcca actgcaataa
    18181 ctgatgcttc aacagccaca agcacaaata ccactaccaa taaccaaaat ccacagcctg
    18241 ttgccacaga agccacaact agtgatctca aacctattga aggtgaaaaa gttgaccttc
    18301 gcatcatgtc aaccacagac ctacacacca atttagttaa ctatgattat tatcaagata
    18361 aagaatctca aaaaataggg ttagcaaaaa ctgctgtcct tattgaagaa gcaaaaaaag
    18421 aaaatcctaa taccgtctta gttgataacg gagatgtcat tcaagggaca cctttaggaa
    18481 cttataaagc aatcgttaaa cctgtcgcag aaaatgaaga acatcctatg taccaagcca
    18541 tgaatgctct tggttatgat gcatcaacac taggtaatca tgagtttaat tacggtttag
    18601 attacctcaa aaaaatcatt gctacagcca accttccaat cctcaacgca aatgttttgg
    18661 atttcaaaac acatcaacca gtttttaaaa cttatgacat cattactaaa accttcaaag
    18721 attcaacagg aagagctgtt gcactaaata ttgggattac aggaattgta cctcctcaaa
    18781 tcttaaactg ggacaaggct aacctagagg gaaaggttat tgtcaaagat tcagttaaag
    18841 caatcgaaga aatcgttcct actatgcgtg ctaaaggagc agatgtcatt ctcgttttat
    18901 cacactctgg cattggagat gatcgatatg aagaaggtga agaaaacgtt ggctatcaaa
    18961 ttgccagcat caagggagtg gatgccgttg ttacgggaca ctcacacgct gaatttccat
    19021 caggtaacgg tactggcttc tatgaaaaat acactggagt tgatggtatc aatggaaaaa
    19081 taaatggaac acctgttaca atggcaggca agtacgggga tcatcttggt attattgatt
    19141 taggacttag ttatactaat ggaaaatggc aagtctccga aagtagtgct aaaatccgta
    19201 aaattgatat gaactcaaca actgctgacg agcgtatcat tgcattggct aaggaagcac
    19261 acgatggcac tatcaactat gttcgccaac aagtaggtac aacaactgcg ccaattacaa
    19321 gttactttgc actagttaaa gatgaccctt cagttcaaat tgttaacaac gctcaacgtt
    19381 ggtacgttgc aaatgaattg aaaggaacac ctgaggcaaa tttaccactt ctatctgctg
    19441 cagcaccatt taaagctgga acacgcggcg atgctaccgc atatactgat attccagctg
    19501 gacctgttgc aattaaaaac gtagctgatc tttaccttta tgataatgtt actgcacttt
    19561 taaaagtgac cggagcagac ttacgtgaat ggttagaaat gtccgctggt caatttaacc
    19621 aaattgaccc taacaataaa gcaccacaaa atattatcaa taccgaatat cgtacctata
    19681 attttgacgt tattgacggc ttgacttata aatttgatat tacacaacct aataaataca
    19741 ataaagatgg aaaagttgtt aatagtcaag ctagtcgagt acgcgattta atgtacaatg
    19801 gtaagcctgt tgcagacaaa caagagttta tgattgtgac caataactac cgtgctagtg
    19861 gtaccttccc tggggctaaa aatgccacaa tgaaccgttt gctgaactta gaaaatcgcc
    19921 aaactattat caactatatt atttcagaga aaaccatcaa tcctacagct gataataact
    19981 ggggcttcac agagtctatt aaagaccttg atttacgatt ccaaacagcc gataaagcta
    20041 aaaatcttgt tactaatagc aaggatattc agtatatcgc tagttctacc aaagatgaag
    20101 gatttggtga ttatcgattt gtctatactg aacaagaaaa agtagacctt actgagccat
    20161 cacatcaaga aaagttaagc caaatagaaa aacaaaaaat tcaccacatg gttgcaactg
    20221 cttatacaaa agcaaaagct tctccaaata ttacaggcac tgtctataca caacagaaag
    20281 aaaaaacatt acctaaaact ggacaagaaa aacaatctta tctggctttc tttggtgtcg
    20341 caatacttgg actcgtggga agtttactta aaaagaaaca aatatagtca tcactaaaaa
    20401 ctgaggccaa tcaacctcag tttttagtct caacaataga ttttctctct aaatagtcag
    20461 atattgagat accactctag cttgtttatc tgacatgatt ttgctaaact gataacaaga
    20521 aatttagatt taaggagtac taaatgccta aattaaccct agtctatatc agtcttagcg
    20581 gcaatactca aagctttgtc aaacgtctta gtgaacaact gtcaacttgg cataactgtc
    20641 aggtttcgac tattaatatc aaggaacaaa atcatcaaac ctttcctgtt gaccaacctt
    20701 tcgtagcact attacccact taccttgagg gaggaaatgg cattgacaat ggcgatcaag
    20761 aaattcttac cattccactt ggtgatttta tagctgcaca caataatgtc aaaaattgta
    20821 tcggaattgt tggctctgga aatagaaatt ttaacaatca gtactgttta actgcaaaac
    20881 aatactctga acggtttgga tttcctatgt taggagactt tgaattgcga ggaacttcta
    20941 gcgacgttga acgtatcgca aatattatcg ttgaaacatt atcgaacttt aaagcctaaa
    21001 ggaaatagta tggaaacaac attaaactac attaaaaccc tgacatcaat cccttcacca
    21061 acagggttta cccaaacaat catgacctat atcatcaaag aattggaagc gtttggctac
    21121 tcaccaattc gcacaaacaa gggaggcgtc atggtttctc taaaaggaaa aaatgatact
    21181 aaacatcgca tgataactgc tcaccttgat acacttggtg ctatggttag ggccatcaaa
    21241 ccagatggtc ggttaaaaat tgaccttatt ggtggatata catacaatgc cattgaagga
    21301 gaaaactgta ctatacacct ctcaaaaaat ggtaaagaaa tttctggaac tgctcttatt
    21361 catcaaacta gtgtccatgt ttacaaagac gctggaactg ctgaacgtaa tcaaacaaat
    21421 atggaaattc gtttagatga gaaagtaaca actgctgacg aaacacgtgc tttaggcatc
    21481 caggtcggtg atttcatttc atttgatccg cgtacaatca taacagacag cggctttatt
    21541 aaatcacgtt acctagatga caaggtatcc gctggtatcc taatggaact tctttctgtt
    21601 tacaagaaag aagacattca acttccttat actactcatt tctactttag tgcctttgaa
    21661 gagctaggac atggagcaaa ttcaagcatc ccaaatgaaa ctgtagaata tctagcagtt
    21721 gatatgggag ctatgggaga cgatcaagaa actgacgaat atactgtctc tatctgtgtt
    21781 aaagatgctt ctggtcctta tcattatgaa ttacgtcaac atcttgtttc tctagctgaa
    21841 aacaataata ttccttataa acttgatatt tatccttatt atggtagtga cgcctccgct
    21901 gccatgcgtg ctggtgcgga agttaaacac gcgctacttg gtgcaggtat tgaatctagt
    21961 cattcttatg aacgtaccca tatcgattct attcaagcaa ctgaactctt agtggatgcc
    22021 tatctcaaaa gcaatatggt ggactaatgg tagccacgct cattattatc aggggaaatt
    22081 cagctagcgg aaagtctaca atagctaagc aacttcaagc cgaattaggg gaaaataccc
    22141 ttctcttatc tcaggattac cttcgtagag agatgttagg aactaaggat ggtgaaaata
    22201 ctactaccat tccccttttg atcaatttac taaattatgg ttaccataac tgttcctaca
    22261 tcatccttga aggcatactt cgttctgatt ggtatacgcc tgtttggaaa catattttaa
    22321 aacataaccc caacaatact tatgcttatt actatgattt aagctttcaa gaaactgtta
    22381 aaagacattc aactcgactt aaatctttag aatttggaga agattctctt gcaagatggt
    22441 ggcttgagaa agatttctta aaagagatac ctgaaaaaat attaacaaaa gctatgagtt
    22501 tagaggatgt caaaaaactt ataatgaccg acgttttacc ttaacattat tgtacgcact
    22561 ataaacaata atgacttctt atcattggag tatattgccc atggatacag tgattgacct
    22621 cttatttatt atcgttccca ttatagccgg atttctggca tgtattagtc tttatcgtta
    22681 ttggaaaaaa gatttcgaaa gcgacgaaga cgacgattaa actcctcgga atcaatgatt
    22741 ccaaggagtt tttctatatt ctcatttaat cagcatttct acgaatacga ttataggttt
    22801 taaggatttg gcccttatgc ttagtgacat agcgataatc ttctttaaga gtagcaatat
    22861 cctttaaagc tttcatgcca ttactcgttt gggcatcttt acgaataggt cggttactcg
    22921 ttgactgccc aaaggcattt tgaacatcta aagaaagcat aaaattaata aataactttg
    22981 cttctttcat agaaggagca ttctttataa ttgcaaccga agatgggaca aaaactgtcc
    23041 cttctgtcgg atatacaata gaaacattgg caccactttt ttgcaaattg acactagggt
    23101 cttcgtaagt cagccccaca atcatttttc cttctgcaac tgattgataa acttctgaag
    23161 agctagaaga tttgatagca ttaatattat gttgtagctt tttaacatag ttccacgctt
    23221 ttggattggt gtaaccaccc ttggccaaga gtatattagt gagttgtgag aaagcactag
    23281 aggaagtatt cggatctgca aaggcaattt taccttttaa ggaaggctgt aataaatctt
    23341 cataactctt gatggtaagt cccttagcta attcgttatt tacaatcaag acactcccat
    23401 ttatagtata aggtgtcgcc gtatcacttg gatggatata gtctggaata acagtatgaa
    23461 cattctttga tacgtaagac tcaaacaatg ccttatgact ttcaaattgc gtataatttc
    23521 ctccaaagaa aatatccgcc ttcaactgct taccctcctt acttaatcta tctattagtt
    23581 gccctgtccc accttgaata agcttaactt ttataccgta tttttcctca aaagctggaa
    23641 tcgttcctgt taaaatggct tgactatttg gacttagaat aactaattct tttggtggaa
    23701 gtagtttaga aggctggcta atactgtatg taaaaacact tataaaaata atggaaacaa
    23761 ccagtagtat ataaataagc cttttcgact gtttttcttt catctttcac ctacctaatc
    23821 catttgttta aaaatatgtt cttccttaaa cgcttttact gtctgaccca agtacttttt
    23881 aaaaacccta tcaaaataac gataatcata aataccaaca gcttctgcaa tttggtaaac
    23941 cattaggtct ggttctctaa acatcaactg aatggcttga ttaatacgat attgatttat
    24001 ataatcactc aaagttatat gaaggtgctt tttagtgact gtgtaaagat aactttcact
    24061 gtaacctaaa tcgtgagcta actggctaat cacaattttc ccatgaaaat ggctatgaat
    24121 ccatgctagc atatctttaa taacttcagg aacttggtca ttagcttgtg gtagaggtaa
    24181 atacaattct gtatgctctt gtaggtgctt ctccttatgt tcctcaattc tagccataat
    24241 tgtttttaaa caatcaatca aagcttgctt ttctagtggt tttgttaaaa attcaagaac
    24301 gccatagtga atagctgatt gcgcatttgg aaaatcagca tagccagata aaataatttt
    24361 ggcataagat tgtcctttgg ttgcctcaaa catatcgaat gctgtcataa ttggcatatt
    24421 aatatcactt aaaacaatgt ctggctgctc ttcctgaatt aattgagctc cttctttacc
    24481 atcttttgct tcaccaacca ctaaaatatt caatgattga taatcaatag catagcgtaa
    24541 ccacttacga ataaggtgct catcctcaac gataagtaat ctatacataa ctcactcctt
    24601 tactatatag ctaactcgaa aagcatcatc tgtcttcgta aactctaaca atactgttga
    24661 aaattgatat tgtaatcgtc tataagaatt aataagccca tgatgcgagt gagttgatct
    24721 taacattgta cgtatggctt gttgcctagc caaactaata cctgatccat tatttgaaac
    24781 agtaaaccaa attccatcac tatcttgcca aatatctatg tttattgcta catcatgacg
    24841 ttcttttaag ccatatttaa tagcattctc aattagcggt aacaagaaaa gttttggtac
    24901 acgcatgtgt tcaagttcag gagatactga aatagtgtaa gttaattctt caaatctcac
    24961 ttgattaatc aaaaggtagg attcaatgat tgctaaatca tccttaagca tagctgcttc
    25021 tgtgctacca ctaaggctat agcgcaataa ttttgttagt tgtataacga ttctttctgt
    25081 taattgtgaa tcatagtgac tggttattaa aattgtctca agggtattat aaaggaagtg
    25141 aggattaaac tgagcttcta acatcctctt ttcaaacaac aacttttgtg tttccaaatc
    25201 aagagttttc tcatgtaaat cttttaactg tgaaaccatc tgattgattt gcacagatag
    25261 atattgaaat tcatcttgac tatcaagctc aatactagat ttttcttggc gggaaatggc
    25321 atccatatct agcaccattt gatttatggc gctagaattt tttactgcga tgcgatcggc
    25381 tagaactcta gatttttgtc gtaaaatcac aaaaataata actgatgata ctagagaaaa
    25441 aaggacgacc agagttactg gtatcagggg acgatacatg taaagtagga tattgtcttg
    25501 cagagctact ttcctaacaa caaaagctcg atgatcgtga aaactaaaat atctagttaa
    25561 gaattgacta tctaccttat ctaaactaga ggagataaaa tcacgattag taaaagtaaa
    25621 gctattattt agctgatcgg caataatcaa gtctgagtta atagccttcg ttggaagtaa
    25681 aaagtctttc ccattcatca ataaaaaggc ataacctgaa acttttttat taactataat
    25741 tggcttaatg agtgcaaggt aatgccctcc ctcactatcc acaataattt ttagtagcct
    25801 ttgatttatt ttagccttat ctaaaacttc actaatgtaa atagacttac taaaaaaatt
    25861 acctaaatgt gaattactag caaacaaaag ttcttgatta ggtgagaata ctaaaagttc
    25921 tgtttgactt tgtaaatgat tccttaaatg ataatattgc ttactcaaat cagcacttcc
    25981 taagtgtcct gagacaaatg gtttcaaact atttttacct aagtcatcta ataaggtatc
    26041 atgtttttga atcaattgct tataactata ggaaactgaa ttaacatctt tataaagacc
    26101 tatctgttga ggcgctaaag aaaaaaggac gaaaagtgca atcaataaca aggataaaat
    26161 taaagactgg cgggaaaaat gtttagagat atcatcttgt agtcgtttct taaagcgttc
    26221 ttccattcta tatcctctca tttccatctc cttttattat agcaaatagc tatccaataa
    26281 agatagtcaa ctcctttcat acaataactt tcaaaagaaa aaaagataag ctaaacaagt
    26341 atcgtcttgt tccacttacc tttaatgcta ggagattatt agcaaatacc taagagacca
    26401 agtacaatac ctaacaccat aataccaaac atgatccagt tgatattgac ttttttacgt
    26461 agaagccaga aggctaatat cgttaccaac agtggcacta cgccaacaaa taattggtca
    26521 aggtaacttt gtatggaagc tgcttcctta gctcctttag ctgctaccgt taagattgtc
    26581 ttgaatttaa catttgaagc agtcattgag cctaccatca ttaaaccaag catacttgaa
    26641 gtcttagtaa caattttaat accacctgat tcatacaatt ttttgataaa ccctgcacct
    26701 acagagtaac ctccatagag actataataa tgaatcagaa aagctggaat gttatacaag
    26761 agtagaaaga caacagctcc catggctgag cctgtacttg ctaaggagat accgatacct
    26821 gccgcaataa cccttagaat tccccaaaag aatgaatcac caacaccaga tataggtccc
    26881 atcaaagaag ccttaactgc ggtgatagca ctagcgtcaa aatcaggatc ttcactgttt
    26941 ttcttttcca tagatgcaac aagacccata ataaaattat taatgtgcat ggtggcatta
    27001 aaccatgttg tatgacggac cagtgcctct gctttatcct tatcagtctt ataaaaacga
    27061 ttgatgactg gtagtaaagt ataaataact cctactgcgt ggtattgtgt tgccccagta
    27121 cgactggcat tcatcgtcca tgagcgccag aagactgagc gaagcatttt acggtcttct
    27181 ttagtcaaat tatcctgtga tttcatgcga agaagtcctc ctcttctgat tctttagagt
    27241 caaaggttgt ctgtttactg atcgcacctc tagtaattgc gtctaattct atatcacgtt
    27301 gtgaactaat gacgcaaata actgctccta gggcagcaac tgcaaccgct ggtaatttta
    27361 aataagctgt taaaacaaaa ccaagcaagt aaaaaacagc taatttattt gtccataata
    27421 atttcatcag catggcaaaa ccaacagctg gaaggaggcc tcccgccgca ctcaaaccat
    27481 tcatcaggtt ttgaggaata tgatggacaa aactattaac aggcccacta cctactaaaa
    27541 taccaatgaa agaaatagaa gcgataatga gatagtaaat aatccatgta ccatagtgaa
    27601 gcatcacaag tttaccttga tggtttgcgg ctgcagcttt atccaccatc ggtgcaaaga
    27661 tattcatgaa gacgtttttc aaaaacataa caacaaatgc tgccaacatt ccaatcggaa
    27721 cagctagagt cattgctgct ttttgatcaa tgtttgaaat aatggtaaaa gttgttgcca
    27781 tcgctgttgc tgttactggc tctgcggcaa taacaccccc aatattaacg tttcccaaaa
    27841 atagcgcttc tagggaggct cccattagga taccaacctt aatatcacct agtagcattc
    27901 ctgttacaag accaactaca agtggtctct ccatcatact ttgacctgtt aagtaatttc
    27961 ccccaaaaca aatcaataca gctaaagctg ccatcgtcgc tggcattatc atatgtcata
    28021 cctcacttta tttgactaac tgactcaagt taaccttagt atttgaaggc aatacttggt
    28081 taaagacgtc taaatctggt aagctggcta attcttttgc ggcttccaac tcttctgtat
    28141 ttaataagag acttgatgtt agtttgactt ttgtagctgg atcagactta tcaaagcgtc
    28201 ctacattagc gacgtttact gcttctaaat cggttatatt tttagcaatt gtacaggcat
    28261 ctttaacaga attgacaatc acaaaaatgc gcttgtcagt tgcacgtgga tctttaaata
    28321 gagctattga ttcttcaact gaacggatta ataatttcat gccattaggc acagccattt
    28381 taagagtcat ctgtgtgatt tcattttttg cagcttcatc attagccacc actaaaagtg
    28441 gtgcatttaa ttctttagtc catacaactg ccacttgccc gtgaatcaaa cggtcgtcaa
    28501 cacgaatttg agtaatcatt ttttcctcct aattattaat cgaaaaagtc atcttctgaa
    28561 cccgctttaa agctaagatc taccagctgc acttgagatt cattgataac ggttacaatt
    28621 tcttctttag ttaattcctc ttcaggtttc aaaaatagta atgacaacaa ggtcgctaaa
    28681 ttgctgttcg ttattaaaaa aatattattt ttcccactat tcattacagc agtcgccatt
    28741 ttctgattaa cgctgccacc taacaaatct gtaaaaataa gcccctgttc atctggtgca
    28801 atctcatcaa taaattctac aatagactta gtatagtcac tatcatcaat gtaagcatct
    28861 attgtctgaa tctcttgtcc cttgcctgtc agaatatcaa tagaactctg caacccactc
    28921 gcaagttttc cgtgactgcc gattaggaat ttccgcttca tctttctcct cctatttatt
    28981 acattaccct aaattagttc tgaaagtttt atcaatactt tgaataagat aacctttact
    29041 atcattctaa caaatgaaag cggtttaaaa atggactatt ctactctata tgtggactat
    29101 tctacataat taatatttaa aaacagaaca ataaaaaggt catgtatcct aatagtttac
    29161 acaaccttta catcactata actttaaact ttctcttata tgcatcacta tttcgaaaga
    29221 gataatggaa taattttttg atagggtatc ttattaacaa cctttattat gagtagagca
    29281 caagcatgtg ttaatgccaa ggtcaccaca aaaatcactt ttccttgatg gtgtgcaact
    29341 aaggaagttg attttaaaca gtaagacact atgccaataa aaagaggggg aaggaagaaa
    29401 taatagacac tcaactgttt taaaggactt aaacgatatt ccttaaacag tgatgtacgt
    29461 atactccagt taaataagaa tactgcacat aaaggagcta ataagaagaa atttttatcc
    29521 aacccttgat taaaataaat aagaacattc tctaaagcta gaagtaaaag gaagagaata
    29581 tacttaagac cacgcgattt ggtaaatagg tcagtattaa aataatcata gagtaaatat
    29641 cctgctagaa gataaactgg agtgtaaaat aatccgtttc gtgttgtctg aaaaattgac
    29701 atataagtgg acatcagggg ataaaatttt gttgactgta taaagacaga ataggtctct
    29761 actgctccaa tacaatagag tacaacaact gtgataaatg tccccctagt tcctacatac
    29821 ttcaataatc cttgaataat gacccaacct agaataacag cagggatgta ccaaagggta
    29881 tgtgacatac ctaaatacaa aaatgccaca ataaaccccg gcaacaaata gatcttcgct
    29941 atatttagag attcaaaaaa gaaataacca taaggcaaat aaatgatagt ccacatgcta
    30001 tactttttaa tcagtttacg aaagtaggag tgattacaca aaccttgctg tatacgacct
    30061 ctcaaaaaga aagcagtaca aatcaaaaaa taaggaacag ccatccgacc taggaaactt
    30121 ttaaaggtaa aatgaatgac atcctgacta aataacctcc cactatgaac taaaatgacc
    30181 agtatggaaa aaaggtattg tagcaaacta atcattgatg tattatgctg cttattatcc
    30241 acgcactgac ttctcttcat ccttaacatt actttctata agttatcatt actatgatac
    30301 caatctcttc agaagaatat ctcaattatc ccaaaaagtc attgaaacgc cttaaatggt
    30361 atctgataat tattgttaat accttatcaa ccatattagg aaataaaaaa gctgatttcg
    30421 caaaagaaaa tcagcttaga ataatttctt gttcagataa ccagtgggaa ctataaactt
    30481 cttcgtcgca ttaaacgata ggtataggct atcaataaca ctgcaattaa catacgtaga
    30541 ctaatcagac agatttcatt agcagaaaaa ctatcaaaga aaaagccatg actaccatta
    30601 cgtgataatt ttaaaagtga agctgaagtg tctgtcgttg atactgagaa aatagttaat
    30661 aacttgttta tcacaccttt attaaacata gccattaaaa agctaggagc aaaaaaggca
    30721 agcgttgaaa cagcaaaaga tgttaatggc gaatttgtga tacttgatat aaagaaatta
    30781 agactggcta taaaaaggag tccaacaaat tgtaagacaa taatatgaac aaaagcagtt
    30841 gatattgtca taggtacagg aaatcccata atattagcca aaggtgatgt ccagtataaa
    30901 ttcagttgaa cgcttgtctc ccaaccacta aaaccaaaat aatctgcaaa taccgtcaga
    30961 atgagggcat atgctaggat aaatgtggaa ataccgatgg tcattgtact agctaactta
    31021 gcccaggtca actgaccacg tccataccga gtcgtcagta gtataggatt aatcttctta
    31081 gccctatctc ctgaaaaaag aggagctgat aagaatagag taaataagac aattaaaata
    31141 taagtactat tacttacttc gaaaagttga ttccaaggag caaagttacc tagctttagt
    31201 tgttttagtg gtagcgcaga acccaactgt ttttgatctt taatatttat tttattaaaa
    31261 tcaattgttt tatcatcttt caattggtta tagtaaatta aaaaatcacg atcaaaagtg
    31321 tctgccacat aataactaaa aacatcaaat ataggggggc gtcctgctgg cggattaaca
    31381 tagtttttaa taatatttgt tactcgttct tcagataaat cccctgaata tttttcagct
    31441 attctacgat taatggcaac cgcttctcga ccatgtattt tatctgcaga atagccacta
    31501 atttgcccaa caaaaaaatg ctggtaaaat agaccaaaaa tagcaagaag gaagataata
    31561 aaagccccaa atgctgcctt acgttgtaga agttttttta attcaaaagg aaaaattgat
    31621 atcatcttac ttactcccct ctaaaatgat agatgtagaa atcttctaga gtcggaacaa
    31681 ccaactgtgc cgaatcactc ggacaaatat cactcactac acgaaatacc cgactatctg
    31741 aaaagacttt ttcattaaca atagcaatat tgggtatctg agataaacct ggttcagtcg
    31801 ttatttccca cacttttccc tcaatagttt tcaacagctg tgcggtgttg ccatgttcga
    31861 taaatttacc attttttaat actataattt ctttggcaac agcttccaca tccgacacaa
    31921 tatgcgttga taaaatgata attttattgg tggataactg actcaaaata tggcgaaatt
    31981 taacccgttc cttgggatca agtccaacag ttggttcatc cagaatgagt atttcaggat
    32041 cattgataag tgcttgagca atgcctaaac gttgcttcat accaccggag tatgatgaaa
    32101 tcttcttttt acctacatcg cctaaaccaa caagctctag taatctagga atttcttgtt
    32161 tagctttctt tttatcaaca ccttttaagg tagcgatata atttaagaaa gcaatgccag
    32221 taaattgatc ataatagcga aaatgttgcg gtaaaaaacc aatgtgacga taaaattctt
    32281 ccgactgctc ctgtccattc caataaatat tcccactatc tgcgaatgta aagtggctca
    32341 tcaaatttaa taaggttgtt ttgccagtac catttgctcc taagagtcca tacaaaccag
    32401 gagtaaactg gtaggaaatc tggttaagta ctattttttt tccgtagctt ttagtgatat
    32461 tatctagctg taataacata gtattgtctc ctttcaataa ttaagacagt tgaaaaatca
    32521 attactaaaa tatagcgact gagctaaagg ttaaaaatga gctttctcac ttactctcta
    32581 aatgattgta aagtaatata gctaaaagat tatttctaag ccctgacgga aagtatgttg
    32641 ttcactattt gtactaatag atgcattggg ataacggtca ataactttca tgacattata
    32701 aaggcctaac cctctatctt ctcccttgct acttgctcca taaccaaaca aacgtgaagc
    32761 atctattctt tcctcgacta tcgaattttc tatgataaaa atttgcttat ggtttaatgt
    32821 aaaataagac atggctatct taggttcatc tgtctctaaa gaaacttcaa tatggtggct
    32881 ataatctatc atattagcca attagagttt tctttgaaca accaacctct cctgtaaaga
    32941 agttttgaag taacggtcta aaatagaact catctttttc tatccttttg atatttatcc
    33001 actatagata ctatatctct cactttattg cgtgaaacca gacaagtcgt tccattttgg
    33061 aagtaaacta atctatctat cctatccact ttaaaaatat ttaagggatt tactaaaaaa
    33121 gagcgatgac attgaaagag cctaggttct tgttttaaaa tatcccccaa agtggctgtg
    33181 aattccattc tatctttttc agtataaagg acaactctat gtgatcttga ggatgtttct
    33241 atataaagaa gatcgttaaa aggtaattgt acctggctgt aacgcgactt aaagtagaat
    33301 gaattttcca ctaagggctt attgcaaatg ccatctacgt acaacaatac ttcctcaatc
    33361 ctttggctaa actcttcctg tgaaagtcct ttatcgatat agtctaaagc tgatacctga
    33421 taacgaaagg ttaaaggcat taattctgaa tgagttgtaa caaaaacaat ctgagcatat
    33481 ggatcaactt gtctaatttg ttttgctacc tctaagccca tctgctcatc attttttatt
    33541 tcaatatcta agaaaaataa attatgtaaa cctatttcat aaattgattc caataattga
    33601 actggtttag caaacgtttc tactgttttg aaatgcagat tgtactgtac tctaatctct
    33661 ttaatgattt tttcaaaatg ggcttgttga acaaaatcat cctcaagtat aaaaatattc
    33721 accttatatc ctctatctta ttacttgaat attaatagtc ac
//
LOCUS       DATRTN010000014        30306 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.014, whole genome shotgun sequence.
ACCESSION   DATRTN010000014 DATRTN010000000
VERSION     DATRTN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30306
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.014"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            327..929
                     /locus_tag="VBE22_000707"
     CDS             327..929
                     /locus_tag="VBE22_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO7202180.1"
                     /translation="MKFLELNKKRHAVKHFNDKPVDFKDVRTAIEIATLAPSANNIQP
                     WKFVVVQEKKSALAEGLPESNCNQINQAQYVIALFTDTDLGQRSRKIARIGRRSLPDD
                     LIGYYMETLPPRYALYSEKQTGEYLSLNAGIVAMNLVLALTDQGISSNMILGFDKAIT
                     NDVLEIDKRFRPEILITVGYSDEKVEPSYRLPVDHIIEKR"
     gene            976..2382
                     /gene="pepV"
                     /locus_tag="VBE22_000708"
     CDS             976..2382
                     /gene="pepV"
                     /locus_tag="VBE22_000708"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEO7202181.1"
                     /translation="MTIDFRAEVDKRKDALMDDLINLLRINSERDDSQADAEHPFGPG
                     PVKALEFFLEMAERDGYETKNVDNYAGHFTFGQGEEELGIFGHLDVVPAGSGWDTDPY
                     EPVIKDNRLYARGSSDDKGPTMACYYALKIIKELGLPTSKKVRFVVGTDEESGWGDMD
                     YYFEHVGLPKPDFGFSPDAEFPIINGEKGNITEYLHFSGENKGAVRLHSFSGGLRENM
                     VPESATARFTSHLDQTTLGASLADFASKHNLKAELSVEDEQYTATVYGKSAHGSTPQE
                     GVNGATYLALYLSQFDFEGPARAFLDVTANIIHEDFSGEKLGVAYEDDCMGPLSMNAG
                     VFQFDETNDDNTIALNFRYPQGTDAKTIQTKLEKLNGVEKVTLSDHEHTPHYVPMDDE
                     LVSTLLAVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPGDENTMHQANEYMPLE
                     NIFRSAAIYAEAIYELIK"
     gene            2479..3063
                     /locus_tag="VBE22_000709"
     CDS             2479..3063
                     /locus_tag="VBE22_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEO7202182.1"
                     /translation="MTDLEKIIKAIKSDLQNQNYTENGIDPLFAAPKTARINIVGQAP
                     GLKTQEARLYWKDKSGDRLRQWLGVDEETFYHSGKFAVLPLDFYYPGKGKSGDLPPRK
                     GFAEKWHPLILKEMPNVQLTLLVGQYAQKYYLGSSAHKNLTETVKAYKDYLPDYLPLV
                     HPSPRNQIWLKKNPWFEKDLIVDLQKIVADILKD"
     gene            3078..3812
                     /locus_tag="VBE22_000710"
     CDS             3078..3812
                     /locus_tag="VBE22_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202183.1"
                     /translation="MRDNHLHTYFSYDCQTAFEDYINGFTGEFITTEHFDLSNPYTGQ
                     DDVPDYSAYCQKIDYLNQKYGNRFKKGIEIGYFKDRESDILDYLKNKEFDLKLLSIHH
                     NGRYDYLQEEALKVPTKELLADYFNRMEFAIGRVEAHVLAHFDYGFRKLNLDVEDLKP
                     FETQLKRIFIKMLSKGLAFELNTKSLYLYGNEKLYRYALEILKQLGCKQYSIGSDGHI
                     PEHFCYEFDRLQGLLKDYQIDENHLI"
     gene            3981..7739
                     /locus_tag="VBE22_000711"
     CDS             3981..7739
                     /locus_tag="VBE22_000711"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEO7202184.1"
                     /translation="MKRKDLFGDKQTQYTIRKLSVGVASVTTGVCIFLHSPQVFAEEV
                     SVSPATTAIAESNINQVDNQQSTNLKDDINSNSETVVTPSDMPDTKQLVSDETDTQKG
                     VTEPDKATSLLEENKGPVSDKNTLDLKVAPSTLQNTPDKTSQAIGAPSPTLKVANQAP
                     RIENGYFRLHLKELPQGHPVESTGLWIWGDVDQPSSNWPNGAIPMTDAKKDDYGYYVD
                     FKLSEKQRKQISFLINNKAGTNLSGDHHIPLLRPEMNQVWIDEKYGIHTYQPLKEGYV
                     RINYLSSSSNYDHLSAWLFKDVATPSTTWPDGSNFVNQGLYGRYIDVSLKTNAKEIGF
                     LILDESKTGDAVKVQPNDYVFRDLANHNQIFVKDKDPKVYNNPYYIDQVQLKDAQQID
                     LTSIQASFTTLDGVDKTEILKELKVTDKNQNAIQISDITLDTSKSLLIIKGDFNPKQG
                     HFNISYNGNNVMTRQSWEFKDQLYAYSGNLGAVLNQDGSKVEASLWSPSADSVTMIIY
                     DKDNQNRVVATTPLVKNNKGVWQTILDTKLGIKNYTGYYYLYEIKRGKDKVKILDPYA
                     KSLAEWDSNTVNDDIKTAKAAFVNPSQLGPQNLSFAKIANFKGRQDAVIYEAHVRDFT
                     SDRSLDGKLKNQFGTFAAFSEKLDYLQKLGVTHIQLLPVLSYFYVNEMDKSRSTAYTS
                     SDNNYNWGYDPQSYFALSGMYSEKPKDPSARIAELKQLIHDIHKRGMGVILDVVYNHT
                     AKTYLFEDIEPNYYHFMNEDGSPRESFGGGRLGTTHAMSRRVLVDSIKYLTSEFKVDG
                     FRFDMMGDHDAAAIELAYKEAKAINPNMIMIGEGWRTFQGDQGQPVKPADQDWMKSTD
                     TVGVFSDDIRNSLKSGFPNEGTPAFITGGPQSLQGIFKNIKAQPGNFEADSPGDVVQY
                     IAAHDNLTLHDVIAKSINKDPKVAEEEIHRRLRLGNVMILTSQGTAFIHSGQEYGRTK
                     RLLNPDYMTKVSDDKLPNKATLIEAVKEYPYFIHDSYDSSDAINHFDWAAATDNNKHP
                     ISTKTQAYTAGLITLRRSTDAFRKLSKAEIDREVSLITEVGQGDIKEKDLVIAYQTID
                     SKGDIYAVFVNADSKARNVLLGEKYKHLLKGQVIVDADQAGIKPISTPRGVHFEKDSL
                     LIDPLTAIVIKVGKVAPSPKEELQADYPKTQSFKESKTVEKVNRIANKTSITPVVSKK
                     ADSYLTNEANLPKTGDKSSKILSVVGISILASLLALVGLSLKRNRHLK"
     gene            7874..8790
                     /locus_tag="VBE22_000712"
                     /pseudo
     CDS             7874..8790
                     /locus_tag="VBE22_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
     gene            complement(8832..9008)
                     /locus_tag="VBE22_000713"
     CDS             complement(8832..9008)
                     /locus_tag="VBE22_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEO7202185.1"
                     /translation="MVKKFAFAKGIATGVVATAATLAGAAFAIKKTIIEPEEEKIAFI
                     EENRKKAARKRVSH"
     gene            9119..10009
                     /gene="miaA"
                     /locus_tag="VBE22_000714"
     CDS             9119..10009
                     /gene="miaA"
                     /locus_tag="VBE22_000714"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEO7202186.1"
                     /translation="MRKIKLIAVVGPTAVGKTALGIELAKTFNGEIISGDSQQVYQKL
                     DIGTAKASKEEQEQAYHHLIDVREVNENYSVYDFVKEAKVAIDTIISKGKIPIIVGGT
                     GLYLQSLFEGYHLGGEVNQETLMAYREKLESLSDEDLFEKLTEQSIIIPQVNRRRAIR
                     ALELAKFGNNLQNSESPYDVLLIGLNDDRQVLYDRINRRVDLMMDNGLLDEAKWLYDN
                     YPSVQASKGIGYKELFPYFSKQIPLEEAVDKLKQNTRRFAKRQLTWFRNRMNVEFIMV
                     GEENYQQKIKRKVSDFLSSK"
     gene            10100..11338
                     /gene="hflX"
                     /locus_tag="VBE22_000715"
     CDS             10100..11338
                     /gene="hflX"
                     /locus_tag="VBE22_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEO7202187.1"
                     /translation="MIETKEEQERVILVGVELQDTENFEMSMEELASLAKTAGANVVN
                     HYYQKRDKYDSKSFIGSGKLEEIKAIVEADEIDTVVVNNRLTPRQNSNLEAELGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQISDIERQLKIVEKNRETVRERRVDSTTFKIGLIGYTNAGKSTIMNVL
                     TDDKQYEANELFATLDATTKQIYLQNQFQVTLTDTVGFIQDLPTELVAAFKSTLEESR
                     HVDLLLHVIDASDPNHEEHEKVVMEILKDLDMIDIPRLAIYNKMDVTEQLNATTFPNV
                     RIAAKKQGSKDLLRRLIVDEIRHIFDEFSIRVHQNQAYKLYDLNKIALLDTYTFEEEY
                     ENITGYISPKQKWKLEEFYD"
     gene            11331..11978
                     /locus_tag="VBE22_000716"
     CDS             11331..11978
                     /locus_tag="VBE22_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEO7202188.1"
                     /translation="MIDYIDLALTYGGFTSLDKVYLEKKLDGLSKQQRLDFITPPPSV
                     INAYFAEIYQKQGPEAATDYYFDLSKALGLFPKHLSFDEEKPFIRLNLSGKSFGFAYL
                     NDQEEASVFSEVKEVITPQLLLEIAQIFPQYKVYRDRSGIRMAKIDFDETESQNITPE
                     TSLLGNVLQLKKDIIKITSFNQEELLELVKTKSGKYYYSSQGRESVIYIDTKSTN"
     gene            12020..12949
                     /locus_tag="VBE22_000717"
     CDS             12020..12949
                     /locus_tag="VBE22_000717"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEO7202189.1"
                     /translation="MEIQFLGTGAGQPAKARNVSSLVLKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKVKKIFITHMHGDHVFGLPGFLSSRAFQANEEQTDLDIYGPVGIKSFV
                     MTALRTSGSRLPYRIHFHEFDESSLGKIMETDKFTVYAEKLDHTIFCMGYRVVQKDLE
                     GTLDAEALKLAGVPFGPLFGKVKNGENVTLEDGREIIAKDYISEPKKGKVITILGDTR
                     KTDASIRLALGADVLVHESTYGKGDERIAKSHGHSTNMQAADIAKQANAKRLLLNHVS
                     ARFMGRDCWQMEEDAKTIFSNTHLVRDLEEVGI"
     gene            12951..13712
                     /locus_tag="VBE22_000718"
     CDS             12951..13712
                     /locus_tag="VBE22_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO7202190.1"
                     /translation="MARTILITGASGGLAQAIINQLPQDDHLIVTGRSREKLEKLYGK
                     RPNTLCLSLDITNDNAVTNTIEKIYGEFGQIDILINNAGFGSFKEFWDYSGEEVKDMF
                     AVNTFATMSIARQIGHKMSLVKSGHIVNIASMAGLIATSKASVYGASKFAVVGFSNAL
                     RLELAEKNVYVTSVNPGPIKTGFFAQADPSGDYLASIGRFALTPEKVAKKLVSILGKN
                     KRELNLPFILAFAHKCYSLFPKTADYFARKVFNYK"
     gene            13709..15907
                     /gene="recJ"
                     /locus_tag="VBE22_000719"
     CDS             13709..15907
                     /gene="recJ"
                     /locus_tag="VBE22_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEO7202191.1"
                     /translation="MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTS
                     AELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIENNETILVYGDYDADGMTSASIMK
                     EALNMMGAEVQVYLPNRFTDGYGPNESVYKYFIEQQDVSLIITVDNGVAGHEAIAYAQ
                     NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPT
                     EMLDLVAIGTIADMVSLTDENRIMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEET
                     IGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKNEERKEIVQTIFDQA
                     MQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEAL
                     DIFQAFDQHRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMSQKKTLTI
                     DSELRFDELSLDTVRDFEKLAPFGMDNKKPVFLLKDFKVSQARVMGQNGAHLKLKLEQ
                     DGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVDGIQLFD
                     IRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIPKNY
                     FISGYGTRDQFAGLYKTIYQFPEFDVRYKLKELSSYLHIPDILLIKMIQIFEELHFVT
                     ITEGIMTVNKEAEKRDISESQIYQELKETVKFQELMTLGTPKEIYDFMMS"
     gene            16059..18623
                     /locus_tag="VBE22_000720"
     CDS             16059..18623
                     /locus_tag="VBE22_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HEO7202192.1"
                     /translation="MRPIVRKKMYKKGKFWVVAGIVTILGGSAILGQDVKAEQAEAVT
                     STISEKTDSSQTISDTSKLTLPVNSSEAMKNSAEPLIKTGFATSVSSNPREIAATPVK
                     TFDASSKVVVKASTAEHSANQTNSNVNQVANDSEVITQQNSTKQLPTVTYSAHVQDIG
                     WQKSVDNATVSGTVGQEKQVEAIKLSIKAPEGITGKLSYKTYVKGQGWQPSVESGQVS
                     GTVGQSRPIEALSINLTDNLQKLYDVYYRVHVQDIGWMAWAKNGAYAGTLGMSKRLEA
                     YEVKFTLKGQSVLTPTIPKEERPVLNYQVKVGQNGWQSNKLEGQMAGTLGESKALDGV
                     KFTLSTLKYGDILYRTHVQDKGWGEILGSQTSKDYQGKRLEAIQLDLSGNLKQNYDIY
                     YRAHVQDKGWMAWQKNNSVAGTVGESKRLEALEVKLVAKASDFSSKTDHSFLEMKHPG
                     LSYQTYLQKDGWKPTVLEGQLGGSIGLSKSIKAIKLNLGSTALGNIEYRTFLNSSGWQ
                     TVVNSGRESNVPNESQQVEAIQMRLTGLLSKTYSVMYRVHMQDYGWQDWTYNNNIAGS
                     TNQGKRIEAIEIKLVETAKIPVTVQTTYKGTGNYNVRVTNVISPGNLKIAIWSDKNNQ
                     DDLKWYTVTPKNHEATLTFNVTNHRDNGKYFIHVYQENASNQKQLLVKTSLQVAHSNY
                     NTPYFSQRDGRWASRRYGMATLGETGCVPTSLAMILSSLKGETVLPTQIADYLYHKTV
                     EFNRGVQGTTSRGILKAAKEWGVTATALGTQANLVQALKDGYHVLAAVQNNVFVLHGS
                     HEIVLKGYNNGLTHVSDPYTPSLSGWYPISQLWKEQSYYSEDRIDIGAPFVKVTDA"
     gene            18746..19264
                     /locus_tag="VBE22_000721"
     CDS             18746..19264
                     /locus_tag="VBE22_000721"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEO7202193.1"
                     /translation="MDLNNYIASIENYPQEGITFRDISPLMADGKAYSYAVREIVQYA
                     ADKDIDMIVGPEARGFIVGCPVAYALGIGFAPVRKPGKLPREVISADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVVAGCAFLVELDGLNGRKAIE
                     GYDTKVLMNFPG"
     gene            19382..20062
                     /locus_tag="VBE22_000722"
     CDS             19382..20062
                     /locus_tag="VBE22_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEO7202194.1"
                     /translation="MTYLEQYQSGQLTLPSALFFHFKSIFKTADDFLVWQFFYLQNTT
                     NLSDLTPSRIATSLDKTVADINRSISNLTSQGLLDVKTIELNHEIEIIFDTSPVFAKL
                     DKLFEEDNQVIIDNKTSDSNRLKDLVGDFERELGRLLSPFELEDLQKTLQEDQTDPDI
                     VRAALREAVFNGKTSWNYINAILRNWRREGLTTLRQIEERKQAREDNQMKDLAISDDF
                     KNAMNLWE"
     gene            20166..20849
                     /locus_tag="VBE22_000723"
     CDS             20166..20849
                     /locus_tag="VBE22_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEO7202195.1"
                     /translation="MDLQLSKRLQKVANYVPKGARLLDVGSDHAYLPIFLLQMGYCDF
                     AIAGEVVNGPYQSALKNVSEHGLTSKIDVRLANGLSAFEEADNIDTITICGMGGRLIA
                     DILNNDIDKLQHVKTLVLQPNNREDDLRKWLAANDFEIVAEDILTENDKCYEILVVKH
                     GHMNLTAKELRFGPFLLSNNTTVFKEKWQNELNKLTFALNSIPNSKIEERAILEDKIQ
                     DIKEVLDES"
     gene            20839..21627
                     /locus_tag="VBE22_000724"
     CDS             20839..21627
                     /locus_tag="VBE22_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEO7202196.1"
                     /translation="MKARELIDVYETYCPQELSMEGDISGLQIGSLDKEIKTVMVALD
                     VRETTVAEAIERQVDLLIVKHAPIFRPLKDLVATPQNKIYIDLLKSDIAVYVSHTNID
                     IVPNGLNDWFCELLDIQYPDILSETSNGYGIGRIGDVRPQSFEFFAWKIKDVFGLDSV
                     RLVSYDKSNPEIQRVAICGGSGQSFYKEAIAKGADVFVTGDIYYHTAQEMITNGLLAI
                     DPGHHIEVLFVSKIATMIEQWKLEKDWDISVLESKAPTNPFYHM"
     gene            21636..22739
                     /locus_tag="VBE22_000725"
     CDS             21636..22739
                     /locus_tag="VBE22_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO7202197.1"
                     /translation="MKKIAIIGAGAVGATLAYYLSKEKDIQVTVFDYGVGQATKAAAG
                     IISPWFSKRRNKSWYRMARLGADFYSNLVTDLQKDGFEIKFYQQTGVFLLKKDESQLE
                     SLFALADKRRLESPLIGDLQILNKSEANTHFPELDGYEQLLYASGGARVEGADLTRIL
                     LEASGVNVIKDEVHFTITDNGFRVQGIDFDKLVLASGAWLAKILDEHNYQVDVRPQKG
                     QLRDYYFSNINTGKYPVVMPEGELDIIPFDNGKVSVGASHENDMAFDLNIDFKVLDKF
                     EEQAIGYFPQLKKADTTSERVGIRAYTSDFSPFFGPVPCMEGAYAASGLGSTGLTVGP
                     LIGYELCQLILNKENQLNLEDYDITKYVKKISR"
     gene            22798..23667
                     /gene="rfbA"
                     /locus_tag="VBE22_000726"
     CDS             22798..23667
                     /gene="rfbA"
                     /locus_tag="VBE22_000726"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEO7202198.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSVLMLA
                     GIKEILIISTPQDLPRFEDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDHV
                     ALVLGDNIYHGPGLSVMLQRAASKESGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PAQPKSNYAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRMQNVQVANLEEIAYRMGYITREQVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            23667..24260
                     /locus_tag="VBE22_000727"
     CDS             23667..24260
                     /locus_tag="VBE22_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEO7202199.1"
                     /translation="MTEQFFDKELTCRPIKVIPGLLEFDIPVRGDNRGWFKENFQKEK
                     MIPLGFPESFFEADKLQNNISFNKKNTLRGLHAEPWDKYVSIADEGRVIGTWVDLREG
                     DSFGNVYQTIIDASKGIFVPRGVANGFQVLSDKAAYTYLVNDYWALELKPKYAFVNYA
                     DPNLGIQWENLEEAEVSEADKNHPLLKDVKPLKKEDL"
     gene            24260..24772
                     /locus_tag="VBE22_000728"
     CDS             24260..24772
                     /locus_tag="VBE22_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEO7202200.1"
                     /translation="MFDLFLTITNELNLIGITPLLMGSVGLEVRTGRDWKARDLDIHV
                     PSDPRGWEAPDEGRIFQFAEINVIMEKLGFNLVDRHEHEYQKEGLSVEFGGLHSLPEF
                     AGIPIDDLEEIETNGAHYFLPTLDQYLKIYQASSKDSYRSDKNNHKDFAKIDYLEKLL
                     SNKMVRNANH"
     gene            24824..25864
                     /gene="rfbB"
                     /locus_tag="VBE22_000729"
     CDS             24824..25864
                     /gene="rfbB"
                     /locus_tag="VBE22_000729"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEO7202201.1"
                     /translation="MYKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGNRA
                     NIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTNFI
                     GTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGNGEGPGEKFTAETKYNPSSPYS
                     STKAASDLIVKAWVRSFSVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLYGE
                     GKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKDAY
                     DHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKDAV
                     EAKYAKTQEVIK"
     gene            complement(25916..29134)
                     /gene="hylB"
                     /locus_tag="VBE22_000730"
     CDS             complement(25916..29134)
                     /gene="hylB"
                     /locus_tag="VBE22_000730"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEO7202202.1"
                     /translation="MEIKKKHRIMLYSALILGTILVNNSYQAKAEELTKTTSTSQIRD
                     TQTNNIEVLQTESTTVKETSTTTTQQDLSNPTASTATATATHSTMKQVVDNQTQNKEL
                     VKNGDFNQTNPVSGSWSHTSAREWSAWIDKENTADKSPIIQRTEQGQVSLSSDKGFRG
                     AVTQKVNIDPTKKYEVKFDIETSNKAGQAFLRIMEKKDNNTRLWLSEMTSGTTNKHTL
                     TKIYNPKLNVSEVTLELYYEKGTGSATFDNISMKAKGPKDSEHPQPVTTQIEESVNTA
                     LNKNYVFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTA
                     STEDKFTKLLDKWNDVTIGNHVYDTNDSNMQKINQKLDETNAKNIKTIKLDSNHTFLW
                     KDLDNLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVN
                     KDIEGSANWWDFEIGVPRSITATLALMNNYFTDAEIKTYTDPIEHFVPDAGYFRKTLD
                     NPFKALGGNLVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYID
                     HTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELM
                     DMSRGRSISREAASSHSAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVF
                     NNLKSYSDIANMNKLLNDSTVATKPLKSNLSTFNSMDRLAYYNAEKDFGFALSLHSKR
                     TLNYEGMNDENTRGWYTGDGMFYLYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDT
                     TKDFMSKHSKDAKEKTGQVTGTSDFVGSVKLNNHFALAAMDFTNWDRTLTAQKGWVIL
                     NDKIVFLGSNIKNTNGIRNVSTTIDQRKDDSKTPYTTYVNGKTVDLKQASSQQFTDTK
                     SVFLESKEPGRNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKH
                     DNKGDSYGYMMVPNIDRTSFDKLANSKEVELLENSSKQQVIYDKNSQTWAVIKHDNQE
                     SLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQTMTKTDQLAI"
     gene            29351..29833
                     /locus_tag="VBE22_000731"
     CDS             29351..29833
                     /locus_tag="VBE22_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO7202203.1"
                     /translation="MTKLATICYIDNGKELLLLHRNKKENDVHEGKWISVGGKLEAGE
                     TPDECAKREILEETHLTVKKMDFKGVITFPEFTPGHDWYTYVFKVTDYEGELISDDES
                     REGTLEWVPYDQVLSKPTWQGDYEIFKWILEDVPFFSAKFVYDEHQNLIEKTVNFYEK
                     "
     gene            29823..30284
                     /locus_tag="VBE22_000732"
     CDS             29823..30284
                     /locus_tag="VBE22_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7202204.1"
                     /translation="MKNRDFRVRDQLETFGVRVSALIIENQKLLLIYAPHLDKYYLPG
                     GALQVGEDSNKAVAREVLEEIGLHSQVGDLAYIVENQFNIKRHHYHSVEFLYFVNLLG
                     QAPESIKEGTHKRHFVWLPIKELTKIDCNPNFLAQDLIEWPGHVVHKIVQN"
BASE COUNT        10068 a         4694 c         6028 g         9516 t
ORIGIN      
        1 acaatatcaa tatccgacga aagataaaac tcagaattcc ttttactaaa taagcaacta
       61 aagtttggat taagatacca gttccaatgt aaacccaaaa ttgatttttt aaagaggtta
      121 aggttagaaa aaaatcttct tttaatgctc gtagcatgaa tagctccttt tttatagtca
      181 ttagatattg taccacaagt ttgtttgttt tttatggtag ttttcaaacc ggtttcactt
      241 ttttatcaat tttgccttca aaagctgaaa cgcattttaa aaagtgatag aatagtaggg
      301 aaaatcaaat agaataggag gtttttatga aatttctaga attaaataag aagcgccatg
      361 ctgtgaaaca ttttaatgat aaaccagtcg actttaaaga tgttagaact gctattgaaa
      421 ttgcgacatt agcgccatct gctaataata ttcagccttg gaagtttgtc gttgtccaag
      481 aaaaaaaatc tgctctagca gaaggtttac cggaaagtaa ttgtaaccaa attaatcaag
      541 cgcagtatgt tattgcttta tttacagata cagatttagg acagagatca cggaaaatag
      601 ctcgtattgg tcgtagatct cttcctgatg atttaattgg ttattacatg gaaactctac
      661 caccacgcta tgctttatat agtgaaaaac aaactggaga gtatctctct ttaaatgcag
      721 gtatcgttgc tatgaattta gtacttgcct tgacagatca aggtattagt tctaatatga
      781 ttttagggtt cgataaagcg attactaatg atgtcttaga gatagataaa cgttttagac
      841 cagaaatatt aattactgtt ggttatagtg atgaaaaggt tgaacctagt tatcgtttgc
      901 cagttgatca tattattgaa aaacgttaat ttttaagcac aaactacata atattttata
      961 gaaggagttg ccgtaatgac aattgatttt agagcagaag tagacaaacg caaagacgct
     1021 ttgatggacg atttaataaa tcttcttcgc attaactcgg agcgtgatga ttcacaagct
     1081 gatgcagagc atccatttgg tcctggacct gtaaaagcct tggaattttt ccttgagatg
     1141 gctgagcgag atggctacga aacaaaaaac gtagataatt atgcgggaca ctttacattt
     1201 ggtcaaggcg aagaagaact aggtattttt gggcatttag atgttgttcc tgcaggcagc
     1261 ggttgggata cagaccctta tgaaccagtt atcaaggata accgtttata tgctcgaggc
     1321 tcatcagacg ataagggacc aacaatggca tgttattatg ctctaaaaat tattaaagag
     1381 cttgggctac caacatctaa gaaagttcgt tttgttgtag gaactgatga agaatctgga
     1441 tggggagata tggattacta cttcgaacat gttggtcttc caaaacctga ttttggtttc
     1501 tctccagatg cagaatttcc aattataaat ggtgaaaaag gtaatattac tgaatattta
     1561 catttttcag gagaaaataa gggagctgtt agactgcata gcttttctgg cggacttcgc
     1621 gaaaacatgg ttccagaatc agccacagca cgtttcacaa gtcatttaga tcagacaaca
     1681 cttggggcta gtctagctga ttttgcaagt aaacataatt tgaaagcaga attatctgta
     1741 gaagatgagc aatatacagc aacagtttat ggtaaatctg ctcatggttc aacaccacaa
     1801 gaaggtgtta atggggcgac ttatttagct ctttatctaa gtcaatttga ttttgaaggt
     1861 cctgctcgtg ctttcttaga tgttacagcc aacattattc acgaagactt ctcaggtgaa
     1921 aaacttggag tagcttatga agatgactgt atgggaccat tgagcatgaa tgcaggtgtc
     1981 ttccagtttg atgaaactaa tgatgataat actatcgctc ttaatttccg ttacccacaa
     2041 gggacagatg ctaaaactat ccaaactaag cttgagaaac ttaacggagt tgaaaaagtg
     2101 actctttctg accatgaaca cacaccacac tatgtaccta tggacgatga attagtatca
     2161 accttactag ctgtctatga aaagcaaact ggtcttaaag gacatgaaca ggttattggt
     2221 ggtgggacat ttggtcgctt acttgaacgg ggtgttgcat acggtgccat gttcccagga
     2281 gatgaaaaca ctatgcatca agctaatgag tacatgcctt tagaaaatat tttccgttcg
     2341 gctgctatct acgcagaagc tatctatgaa ttaatcaaat aaaataatcc ttaaactaaa
     2401 tatgtgatca atgataaagg gtggtgaaga catgaaagtg tctttgcctc ttttcataag
     2461 gttagatttg gagactttat gactgacttg gaaaaaatta ttaaagcaat aaaaagtgat
     2521 ttacagaatc aaaattatac agaaaatggt attgatcctt tgtttgctgc tcctaaaaca
     2581 gctaggatca atattgttgg ccaagcacct ggtttaaaaa ctcaagaagc aagactctat
     2641 tggaaagata aatctggaga tcgtctacgc cagtggcttg gagttgatga agagacattt
     2701 taccattctg gaaaatttgc tgttttacct ttagattttt attacccagg caaaggaaaa
     2761 tcaggagatt taccccctag aaaaggtttt gcggagaaat ggcaccctct tattttaaaa
     2821 gaaatgccta atgttcaatt gaccttgcta gttggtcagt atgctcagaa atattatctt
     2881 ggaagctccg cacataaaaa tctaacagaa acagttaaag cttacaaaga ctatctaccc
     2941 gattatttac ccctggttca cccatcaccg cgaaatcaaa tttggctaaa gaagaatcca
     3001 tggtttgaaa aagatctaat cgttgattta caaaagatag tagcagatat tttaaaagat
     3061 taaggatagg agttggtatg agagataatc atctacacac gtatttttcc tatgattgtc
     3121 aaacggcatt tgaggactat attaatggtt ttacaggtga atttatcacg acagaacatt
     3181 ttgatttatc aaatccttac accggtcaag acgatgttcc tgattatagt gcttattgtc
     3241 aaaaaataga ttatcttaat cagaaatatg gaaatcgatt taaaaaagga attgaaatcg
     3301 gttattttaa agatagggaa tcagatattt tagattattt aaaaaataaa gaatttgatt
     3361 taaaactatt gtcaatccat cataatggta ggtatgatta tctgcaagaa gaagctctga
     3421 aagtaccaac aaaggagctt ttagcagatt actttaatcg tatggaattt gccataggcc
     3481 gtgtggaagc gcacgtttta gctcactttg attatggttt tcgtaagtta aacttagatg
     3541 tagaagattt aaaaccgttt gaaacgcaat tgaagcgcat tttcataaag atgttatcta
     3601 aggggttagc ttttgaacta aataccaaat ccctttatct atatgggaat gaaaaacttt
     3661 atcgctatgc tttagagata ctcaaacagc ttggttgtaa acaatactct ataggctctg
     3721 acggtcatat tcctgaacat ttttgttatg aatttgatag acttcaaggt ctgctaaagg
     3781 actatcaaat tgatgaaaat catttgatat gaggaaattt ttgataaaaa agctaggcaa
     3841 tattgcttag cttttttgta atgctattga tagttttagt gaaaatttca aaaaaataaa
     3901 gaaatcattt acttgttgca agcgcttgcg taaattgtta tgattttatt ggtaacaatt
     3961 cattaaaaaa ggagaatgat atgaaaagaa aagacttatt tggtgataaa caaactcaat
     4021 acacgattag aaagttaagt gttggagtag cttcagttac aacaggggta tgtatttttc
     4081 ttcatagtcc acaggtattt gctgaagaag taagtgtttc tcctgcaact acagcgattg
     4141 cagagtcgaa tattaatcag gttgacaacc aacaatctac taatttaaaa gatgacataa
     4201 actcaaactc tgagacggtt gtgacaccct cagatatgcc ggataccaag caattagtat
     4261 cagatgaaac tgacactcaa aagggagtga cagagccgga taaggcgaca agcctgcttg
     4321 aagaaaataa aggtcctgtt tcagataaaa ataccttaga tttaaaagta gcaccatcta
     4381 cattgcaaaa tactcccgac aaaacttctc aagctatagg tgctccaagc cctaccttga
     4441 aagtagctaa tcaagctcca cggattgaaa atggttactt taggctacat cttaaagaat
     4501 tgcctcaagg tcatcctgta gaaagcactg gactttggat atggggagat gttgatcaac
     4561 cgtctagtaa ttggccaaat ggtgctatcc ctatgactga tgctaagaaa gatgattacg
     4621 gttattatgt tgattttaaa ttatctgaaa aacaacgaaa acaaatatct tttttaatta
     4681 ataacaaagc agggacaaat ttaagcggcg atcatcatat tccattatta cgacctgaga
     4741 tgaaccaagt ttggattgat gaaaagtacg gtatacatac ttatcaaccc ctcaaagaag
     4801 ggtatgtccg tattaactat ttgagttcct ctagtaacta tgaccactta tcagcatggc
     4861 tctttaaaga tgttgcaacc ccctcaacaa cttggccaga tggtagtaat tttgtgaatc
     4921 aaggactata tggaaggtat attgatgtat cactaaaaac taacgccaaa gagattggtt
     4981 ttctaatctt agatgaaagt aagacaggag atgcagtgaa agttcaaccc aacgactatg
     5041 tttttagaga tttagctaac cataaccaaa tttttgtaaa agataaggat ccaaaggttt
     5101 ataataatcc ttattacatt gatcaagtgc agctaaagga tgcccaacaa attgatttaa
     5161 caagtattca agcaagtttt acaactctag atggggtaga taaaactgaa attttaaaag
     5221 aattgaaagt gactgataaa aatcaaaatg ctatacaaat ttctgatatc actctcgata
     5281 ctagtaaatc tcttttaata atcaaaggcg actttaatcc taaacaaggt catttcaaca
     5341 tatcttataa tggtaacaat gtcatgacaa ggcaatcttg ggaatttaaa gaccaacttt
     5401 atgcttatag tggaaattta ggtgcagttc tcaatcaaga tggttcaaaa gttgaagcca
     5461 gcctctggtc accgagtgct gatagtgtca ctatgattat ttatgacaaa gataaccaaa
     5521 acagggttgt agcgactacc ccccttgtga aaaataataa aggtgtttgg cagacgatac
     5581 ttgatactaa attaggtatt aaaaactata ctggttacta ttatctttac gaaataaaaa
     5641 gaggtaagga taaggttaag attttagatc cttatgcaaa gtcattagca gagtgggata
     5701 gtaatactgt taatgatgat attaaaacgg ctaaagcagc ttttgtaaat ccaagtcaac
     5761 ttggacctca aaatttaagt tttgctaaaa ttgctaattt taaaggaaga caagatgctg
     5821 ttatatacga agcacatgta agagacttca cttctgatcg atctttggat ggaaaattaa
     5881 aaaatcaatt tggtaccttt gcagcctttt cagagaaact agattattta cagaaattag
     5941 gagttacaca cattcagctt ttaccggtat tgagttattt ttatgttaat gaaatggata
     6001 agtcacgctc aacagcttac acttcctcag acaataatta caattggggc tatgacccac
     6061 agagctattt tgctctttct gggatgtatt cagagaaacc aaaagatcca tcagcacgta
     6121 tcgccgaatt aaaacaatta atacatgata ttcataaacg tggcatgggg gttatacttg
     6181 atgtcgtcta taatcacact gcaaaaactt atctctttga ggatatagaa cctaattatt
     6241 atcactttat gaatgaagat ggttcaccaa gagaaagttt tggaggggga cgtttaggaa
     6301 ccactcatgc aatgagtcgt cgtgttttgg ttgattccat taaatatctt acaagtgaat
     6361 ttaaagttga tggtttccgt tttgatatga tgggagatca tgatgcggct gcgattgaat
     6421 tagcttataa agaagctaaa gctattaatc ctaatatgat tatgattggt gagggctgga
     6481 gaacattcca aggcgatcaa ggtcagccgg ttaaaccagc tgaccaagat tggatgaagt
     6541 caaccgatac agttggcgtc ttttcagatg atattcgtaa tagcttgaaa tctggttttc
     6601 caaatgaagg tactccagct ttcatcacag gtggcccaca atctttacaa ggtattttta
     6661 aaaatatcaa agcacaacct gggaattttg aagcagattc gccaggagat gtggtgcagt
     6721 atattgctgc acatgataac cttaccttgc atgatgtgat tgcaaaatca attaataaag
     6781 accctaaggt agctgaagaa gagattcata gacgtctgcg tttaggaaat gtaatgattt
     6841 taacatctca agggacagca ttcattcatt ctggtcaaga gtatggtcgt acgaagcgtt
     6901 tacttaaccc tgattacatg acaaaagttt cagatgacaa attgcctaat aaagcaacac
     6961 ttattgaagc tgttaaagaa tatccatatt ttattcatga ttcatatgat tcttcagatg
     7021 ccattaatca ttttgattgg gcagcagcca cagataataa caaacaccca atttcaacga
     7081 aaacacaggc ctatacagca ggtttaatca cattaaggcg ttcaacagat gctttccgga
     7141 aattgagcaa agcagaaatt gatcgtgagg ttagcttgat tacagaggta ggtcaaggtg
     7201 atattaaaga aaaagatttg gttattgctt accaaacaat agattctaaa ggtgatattt
     7261 acgcagtatt tgttaatgct gatagtaaag ctagaaacgt tttactaggt gaaaaatata
     7321 aacacctttt aaaagggcaa gtaattgttg atgctgatca agcggggatt aaaccaatct
     7381 caactcctag aggtgttcat tttgaaaaag atagtttgct gattgatcca ttaacagcaa
     7441 ttgtgattaa agttggcaaa gttgctccta gccctaagga ggaattgcaa gcagattatc
     7501 ccaaaacaca atctttcaag gaatctaaaa cggtagaaaa agtaaataga atagctaata
     7561 agacctcaat aactcctgta gtttctaaga aggccgattc atatctgaca aatgaagcta
     7621 atttgccaaa aactggagat aagtcatcaa aaatactaag tgtagtagga ataagcattc
     7681 tagcaagtct acttgctcta gtaggtctct ctttaaagag gaatcgtcac ttaaaataaa
     7741 agcaaacttt agaagagctt aaaaaagaag ttagtcaatg gactagcttc ttttcattaa
     7801 gttaataaaa tcacttatca gcaaaatcta attgtgtttt aatagatgta aacgtttttt
     7861 tatgaggtat atgatgaaaa attgggaaaa accaccatta gtgatggcgg gattagtatt
     7921 agggctatta gctttgggga accttctaga gggatatggc acctatgttc gctattgttt
     7981 agggctagta gcacttgttt tttggatttt ttaatcaaag gaatattaaa aaataaaaaa
     8041 gaaagcagga aggagttaag caatcctttg attgcttctg tctttactac tttttttatg
     8101 gcaggtatga ttttatcaac ctatatctta ttatttagaa gtttaggaat atgggtagcc
     8161 gttctgtcaa aaggtgtatg gtggttgtca tttattgcgt taatcattca tatggctatt
     8221 ttttcatgga aatatttgag acacttttcg atggctaatc tttttccttc ttggtctgtc
     8281 ctctatgttg gtataggggt agctagcctg acagcaccaa ttagtggaca attcactatt
     8341 gggaaaatag tcttttggta tggttttatt gcaactttag tattattacc atttctattc
     8401 attaaagcat ataaaatcgg gctaccttcg gcagttaagc ctaatataac tactatttgc
     8461 gctccaatgt cgctaatcac agcgggatat gtcaatagtt ttgtgtcacc aaatagaggt
     8521 ttattactgc tattaatcgt aatggcacaa tttctttatt ttttcattct ttttcaggtg
     8581 cctaaattac tgattggtga ttttacacca ggtttctcag cctttacttt cccccttgtt
     8641 atctcagcta cctcacttaa attaagtatt cagcatctat cattaccagt tgatatacag
     8701 gggctagtcc actttgaaat tgggacaaca actcttattg ttatgatagt aatggtacgt
     8761 tatattttct ttttaaggcg aacaatttaa tataaaataa ctcccttact agctagtaag
     8821 ggagttattg tttaatgaga gacacgttta cgtgcagctt ttttacgatt ttcttcgata
     8881 aaagctattt tttcttcctc aggttcaata attgtttttt tgatagcaaa tgcagcacct
     8941 gcaagagtag cagctgttgc aacgactcct gttgctattc ctttagcaaa tgcgaatttt
     9001 ttaaccataa taattctcca atctatgatg aagactctgc ttctatctat gatataatta
     9061 tggtaacgaa atactagtta aatttcaagt aaaaaccttt atagaattga gaagtatcat
     9121 gagaaaaatc aagcttatag cagttgttgg tccaactgcg gttgggaaga cagcattagg
     9181 aattgaatta gctaaaactt tcaatggcga aatcattagt ggtgatagtc agcaagttta
     9241 tcagaagtta gatatcggaa cggctaaagc tagtaaagaa gagcaagaac aagcttatca
     9301 tcacttgatt gatgttcgtg aagtaaatga gaactattcg gtttatgatt tcgtcaaaga
     9361 ggcaaaagta gctattgata cgattatttc taaggggaaa attcctatta ttgttggagg
     9421 tactggccta tatttacaga gtttgtttga gggctatcat ttaggaggcg aagtcaatca
     9481 agaaactctt atggcctatc gtgaaaaatt agagtctcta agtgatgaag atttatttga
     9541 aaagttgaca gaacaatcca ttattatacc acaagttaat cgtcgacgtg ccattcgtgc
     9601 attagaattg gcgaagtttg ggaataattt gcaaaatagt gaaagtccgt atgatgtttt
     9661 attaattgga ttgaatgatg atcgtcaggt gctttatgat cgtatcaata gacgagttga
     9721 tttgatgatg gacaatggtc tcttagatga agctaaatgg ctatacgata actacccgag
     9781 tgttcaagca agtaaaggaa ttggctataa agaattattt ccttactttt caaagcaaat
     9841 accattagaa gaagctgttg ataaactaaa acaaaatacc agacgttttg caaaacgcca
     9901 attaacatgg tttagaaacc gtatgaatgt cgaatttatt atggttggtg aggaaaatta
     9961 tcaacaaaaa atcaagcgaa aagtcagtga ttttttatca tcaaagtaaa taaagccgtt
    10021 tctcgcattt aaagggcatt atgttataat aaagattacg tatttagaac aagaaaaaaa
    10081 tattgaaagg agattatagg tgatagaaac taaagaagag caagaacgtg ttattttagt
    10141 tggggtagaa cttcaagata ctgagaattt tgaaatgtcc atggaagaac ttgctagctt
    10201 agctaagaca gcaggagcaa atgttgttaa tcattattat caaaaaagag ataaatatga
    10261 tagtaaatca tttatcggat ctggtaaatt agaggagata aaagctattg tagaagcaga
    10321 tgagattgat accgttgtgg ttaataatcg tttgactccg cgacaaaact ctaatttgga
    10381 agctgaactt ggtgttaaag ttattgaccg tatgcaactt attttagata tctttgctat
    10441 gcgtgctaga agtcatgaag gaaaattaca ggttcatcta gcgcaattga aatatatgtt
    10501 accacgctta gttggacaag gtattatgct tagtcgtcaa gcaggaggaa tcggaagccg
    10561 aggtccagga gaatctcagt tagagttgaa tcgtcgttcc attcgtcatc aaatttctga
    10621 tatcgaaaga caattgaaga ttgtagaaaa aaatcgcgag acagttcgag aaagacgtgt
    10681 tgattcgaca acctttaaaa ttggattaat tggatataca aatgctggca agtcaactat
    10741 tatgaatgtt ttaaccgatg ataagcaata tgaagccaat gaattatttg caacgttaga
    10801 cgctacaacc aagcagatct atttacaaaa tcaatttcaa gtcactttaa cagatactgt
    10861 tggttttatt caagatttac ctacagagtt agttgcggct ttcaagtcta ccttagaaga
    10921 aagtcgacat gtcgatttat tacttcatgt tattgatgct agtgacccta atcatgaaga
    10981 acacgaaaaa gttgttatgg agattcttaa agacttagat atgatagata ttcctcgttt
    11041 agcgatatac aacaagatgg atgtcactga gcagctgaat gcaacaactt ttcctaatgt
    11101 gagaattgca gccaaaaaac aagggagtaa ggatttatta cgccgtctta ttgtggatga
    11161 aatacgacat atctttgacg aattttcgat tagagtgcat caaaatcaag catataagct
    11221 ctatgatctc aataaaatag cattgcttga tacctacacc tttgaagaag aatacgagaa
    11281 tattacaggc tatatttctc ctaaacaaaa atggaagctg gaagaatttt atgattgatt
    11341 atattgattt agctttgact tatggtggtt ttacgagctt agataaagtt tatctagaga
    11401 aaaaactaga tggcctctct aagcaacaac gacttgattt tatcacccca cctcctagtg
    11461 tgattaatgc ttattttgct gaaatatatc aaaagcaagg gccagaagct gcaactgatt
    11521 actattttga tctttccaaa gccttgggct tgtttccaaa acatttatcg tttgatgaag
    11581 aaaaaccttt tattcgtttg aatttatcag gaaaatcatt tggttttgct tacctaaatg
    11641 atcaagaaga agcgagtgtt ttttctgaag ttaaagaagt cattacaccg cagttactct
    11701 tagaaattgc acagattttt cctcaatata aagtctatcg agatcggtct ggtatcagaa
    11761 tggcaaaaat agattttgac gaaacagaat ctcaaaatat tacaccagaa acaagtttac
    11821 tagggaatgt cttacaactt aaaaaagata ttattaaaat aactagtttc aatcaagaag
    11881 agctattaga gttggttaaa acaaagtcag gtaaatacta ttattctagt caaggacgtg
    11941 agtcagttat ttatattgat actaaatcta ctaattaaat ttattaagtt atggaaggat
    12001 tataaaaaag tcaacaagta tggaaattca atttttagga actggagctg gtcaaccagc
    12061 gaaagctcgt aatgtttcaa gtttagtctt gaaattatta gatgaaatta acgaggtttg
    12121 gatgtttgat tgtggagagg gtacacagcg tcaaatttta gaaactacga ttaaacctcg
    12181 taaagttaaa aaaattttta tcacgcacat gcacggagac catgtttttg gtcttccagg
    12241 ttttttatcc agtcgggctt ttcaagccaa tgaagagcag acagatttag atatttatgg
    12301 accggttggt ataaaaagtt ttgttatgac tgccttaagg acatcaggtt cacgtttacc
    12361 ttatcgaatc cattttcacg agtttgatga atcatcactc ggaaaaatta tggaaacaga
    12421 taaatttacc gtttatgcag aaaagctaga tcatactatc ttttgtatgg gatatcgtgt
    12481 tgtgcaaaaa gatcttgagg gcactttaga tgctgaagca ttgaaactag caggtgttcc
    12541 atttggtcct ctttttggga aagtcaaaaa tggtgagaat gttaccttag aagatggtag
    12601 agaaattatt gctaaagatt atatttctga accgaaaaag ggtaaagtaa tcactatttt
    12661 gggagatact agaaaaacag atgcaagtat taggttagct ttgggcgctg atgttttagt
    12721 tcacgaatct acttatggta agggtgatga gcgaattgct aaatcgcatg ggcattcaac
    12781 caatatgcaa gcagctgata tcgctaaaca agcaaatgct aaacgactat tattaaatca
    12841 tgttagtgct cgttttatgg ggcgagattg ttggcaaatg gaagaagatg caaagacgat
    12901 tttttcaaat acccatcttg ttagagattt ggaagaagta ggtatataaa atggcaagaa
    12961 cgatactgat tacaggtgct tctggtggtt tagcacaggc aattattaac caattacctc
    13021 aagacgacca tttaattgtc acagggagat caagagaaaa acttgaaaag ctttatggaa
    13081 agagaccaaa taccttatgt ttatcactag acataactaa tgacaatgct gtaacgaata
    13141 cgattgaaaa aatttatggt gaatttggtc aaattgatat tttaattaat aatgctggat
    13201 ttggttcttt taaagaattt tgggattata gtggtgaaga agtcaaagac atgtttgctg
    13261 tgaatacatt tgcaacgatg tctatagctc gtcagatagg acataagatg tctcttgtaa
    13321 aatctggtca tatcgttaat attgcaagca tggctggcct catagcaaca agtaaagcta
    13381 gcgtttatgg ggctagtaag tttgctgtcg ttggattttc gaatgctctt cgtttagaat
    13441 tagcagaaaa aaatgtttat gtgacaagtg taaaccctgg accaatcaaa acaggatttt
    13501 ttgctcaggc tgatccaagt ggcgattatt tagcaagtat aggtcgtttt gctctaacac
    13561 cagaaaaagt agctaaaaaa ctagtttcta ttcttggaaa aaataagaga gaattaaatc
    13621 ttccttttat attagctttt gcgcataaat gttatagttt atttccaaaa acagctgatt
    13681 attttgctag aaaggtattc aattataaat gatatctgct aagtatagtt gggtcctcaa
    13741 taaccaaaag ccagacgctg gcttttttga ggcttcaaaa aaagaaaaga taagtgaagc
    13801 ggtggcttcc ttaatttatt cgcgaggtat taagacatca gcggaattac atcatttttt
    13861 acaaactaat ttggaaaatc tccatgatcc ttatttgtta aatgatatgg ataaagctgt
    13921 taatcgtatt agacgagcta ttgaaaataa tgaaactatt ttagtttatg gagactatga
    13981 tgctgatgga atgacatcag catcaatcat gaaagaagct ttaaatatga tgggagctga
    14041 agtccaggtt tatctcccaa atcgctttac agatggttat ggtcccaatg aaagtgttta
    14101 taaatatttt attgaacaac aagatgtttc attaatcatt acggttgata atggtgtggc
    14161 agggcatgaa gcaattgcat atgctcaaaa tcaaggtgtg gacgtggtag ttactgatca
    14221 tcacagtatg ccagcagatt taccttgtgc ttatgctatt attcacccag aacatcctga
    14281 tgctaattat ccatttcctt atttagcagg gtgtggtgtt gcttttaaag tagcttgtgc
    14341 tctcttagaa acaatcccga cagaaatgtt ggatttggtt gctattggga cgattgcaga
    14401 tatggttagc ctaactgatg aaaatcgtat catggtaaaa gctggacttg aagttatgaa
    14461 agatagtgaa agaataggct tacaggagct aatatctcta tcaaatattg atttaaaaac
    14521 actaaatgaa gaaactattg gatttaaaat tgctccacaa ttaaacgctt taggaagact
    14581 tgatgaccct aatccagcta ttgaattact gacagggttt gatgatgaag aatctcaagc
    14641 aatagcccaa atgattgatc aaaaaaatga agagcgaaaa gaaatcgttc aaactatctt
    14701 tgaccaagct atgcaaatgc ttgatcaaac aaagcctgta caagttttag caaaagaaaa
    14761 ttggcatcca ggggttttag gtattgtggc gggtcgtatt ttagagagaa ctggacaacc
    14821 tgttattgtt ttgaatattg aagatggtat tgctaaagga agtgccagaa gtgtagaagc
    14881 acttgatatt tttcaagctt ttgaccagca tcgagaatta ttcattgctt ttggaggtca
    14941 cagcggagca gcaggcatga ctttagagga aagtaaggtg ggtgatcttt ctcaagtttt
    15001 atgtgactat atctctaaaa aacaattgga catgagtcag aaaaaaactc tgacaattga
    15061 tagtgagcta cgttttgacg aacttagttt agacactgtt agagattttg aaaagttagc
    15121 gccatttgga atggataata aaaagccagt ctttttgtta aaagatttta aagtgagtca
    15181 agctagagtc atggggcaaa acggtgctca tctgaaatta aaactagaac aagatggaca
    15241 agcacttgat ttggtagcat ttaatatggg gagtcaacta caagagttcc aacaagcaca
    15301 acatttggag ttagctgtca ctttatctgt taatcaatgg aatggtgcca caaccctaca
    15361 attaatgttg gaggatgcgc gtgtagacgg tattcaatta tttgatatta gaagcaaagc
    15421 aagtagctta cctcatggag tgccaatcct gtctcaagaa gagcagagca aagaagttat
    15481 tcttttgact gttcccgatc atccacaaga attaaaacaa atgactcaag gtaaacagtt
    15541 tgatgccatc tattttaaaa atgagatacc aaaaaattat tttatatctg gttatggtac
    15601 acgtgatcag tttgctggtt tatataaaac aatatatcaa ttcccagaat ttgacgttcg
    15661 ttataagtta aaagagctca gttcctacct tcatatccct gatattttat taattaaaat
    15721 gattcaaatt tttgaagaac tacattttgt tacgattaca gaggggatta tgacagtcaa
    15781 taaagaagca gaaaagcgag atatctctga gagtcaaatt tatcaagaat taaaagagac
    15841 agttaagttt caagaattaa tgactttagg gacacctaaa gaaatatatg attttatgat
    15901 gagttgataa tatactaaag aagccacttt ttaagaggtt tttttagcat ataaataagt
    15961 tatttcaata ataatattgc aaatattatt aaaatgtaat aaaattaaga agataatgga
    16021 atttttgata aagaatatta aaggtgagga taaaatctat gagacctatc gttagaaaaa
    16081 agatgtacaa aaaaggtaaa ttttgggtcg ttgcaggtat cgttactatt ttaggtggta
    16141 gtgcaattct tggtcaagat gtcaaggcag aacaggcaga agcagtaact tcaacgatct
    16201 cagagaaaac tgatagcagt cagactattt cggatacttc caagttaacg ttacctgtta
    16261 attcctcgga ggctatgaaa aatagtgcag agcctttaat aaaaacaggc tttgctacat
    16321 ctgtttcatc taatcctaga gagatagcag ctaccccagt caaaaccttt gatgctagct
    16381 caaaagttgt tgtaaaagct agtactgctg agcactcagc aaatcaaaca aattctaatg
    16441 ttaatcaagt agctaatgat agtgaagtta ttactcaaca aaattcaaca aagcaattac
    16501 caacagtgac ttactctgct catgttcaag atattggatg gcaaaagtcg gttgataatg
    16561 caacagtttc aggaacagta ggacaagaaa agcaggttga ggcaataaaa ttgtctatta
    16621 aagcacctga aggaataaca ggaaaactta gctataaaac ttatgttaaa ggacaaggtt
    16681 ggcaaccttc agtagagagt ggtcaagtta gtggtactgt tggtcagagt agacctattg
    16741 aagcactaag cattaacctt actgataatt tacaaaaatt atatgatgtc tattatcgtg
    16801 ttcatgttca agatataggg tggatggctt gggctaaaaa tggagcttac gctggaactt
    16861 tgggaatgtc aaagcgcctt gaagcttacg aagtaaaatt tacccttaaa ggacaatctg
    16921 ttttaacacc aactatacct aaggaagaga gacctgtctt aaattatcag gttaaggtag
    16981 gccaaaatgg ttggcaatct aataaacttg aaggacaaat ggcgggaact ttaggagaat
    17041 ccaaagccct agatggagta aaattcactc ttagcacctt gaagtatggg gacattctct
    17101 atcgtactca tgtacaagat aaaggttggg gagaaatttt aggtagtcaa acaagtaagg
    17161 attatcaagg aaaaagacta gaagctattc aattggactt aagtgggaat ttaaaacaaa
    17221 attatgatat ttattatcgc gctcacgtcc aagataaagg ttggatggct tggcaaaaaa
    17281 ataatagtgt agcaggtaca gtcggagaat cgaaacgttt agaggctttg gaagtaaaac
    17341 tagtagctaa agcttcagat tttagttcca aaacagatca ttcattctta gaaatgaagc
    17401 atcctggatt aagttatcaa acttatcttc aaaaagatgg ttggaaacca acggttttgg
    17461 aaggccaatt aggaggtagt attggacttt caaaatctat aaaagccatc aaacttaact
    17521 taggtagtac agctctagga aatatagaat atagaacttt tcttaatagt tcaggttggc
    17581 aaactgttgt taattcaggg agagaaagta atgtaccaaa tgaatctcaa caagtagaag
    17641 ctattcagat gcgattgaca ggtctgctat cgaaaaccta tagtgtgatg tatcgagttc
    17701 acatgcaaga ttatggttgg caggattgga cttataacaa taacatcgct ggtagtacta
    17761 atcaaggaaa acgaattgaa gcaatcgaaa ttaaattagt tgagactgct aaaataccgg
    17821 taactgtcca aacgacctat aagggaactg gaaactataa tgtacgagtg acaaatgtta
    17881 ttagtcctgg gaatttaaaa attgctattt ggtcagataa aaataatcag gatgacctta
    17941 agtggtatac cgtcacaccc aaaaatcatg aagctacatt gacatttaat gttacaaatc
    18001 atagagacaa tggtaaatac tttatccatg tttatcagga aaatgcttct aatcaaaaac
    18061 aattattggt aaaaacttct ctacaagtcg cacattcaaa ttataatact ccttacttta
    18121 gccaacgtga tggaaggtgg gcaagtcgaa gatatggtat ggcaacttta ggagaaactg
    18181 gttgtgtacc aacttctcta gctatgattt tgtcttcttt gaaaggagaa actgttttac
    18241 caacgcaaat tgcagattac ctttaccata agacagttga atttaatcgt ggtgttcaag
    18301 gaacaacttc tagaggaatt ttaaaagcag ccaaagaatg gggtgtaact gcaactgcat
    18361 taggaactca agctaacctt gtacaagcgt tgaaggatgg ttatcatgtc ctagcggcag
    18421 tacaaaataa tgtttttgtt cttcatggaa gtcatgaaat cgttctcaaa ggatataata
    18481 acggactgac acatgtctct gacccttaca caccgtcctt gtctggttgg tatccaattt
    18541 cacaactttg gaaagaacag agctattata gtgaggatag aattgatatt ggagcacctt
    18601 ttgtcaaggt aacagatgcc taatttttaa aaccatatgc aaaactagca tatggtttta
    18661 aagtttccag tactatttta tttaatttta tgatataatg aagagtaaaa cattttaggg
    18721 ttttcccctt agaaagacaa gtgaaatgga tttaaataat tacatcgctt caattgaaaa
    18781 ctatcctcaa gaaggaatta ctttccgtga tatctcccca ttaatggctg atggtaaagc
    18841 atacagctat gctgttcgtg aaattgtaca atatgctgct gataaagata ttgatatgat
    18901 tgttggacca gaagctcgtg gctttattgt tggttgccca gttgcttatg ctcttggtat
    18961 tggttttgct ccagtccgta aaccaggtaa attaccacgc gaagttattt cagctgacta
    19021 tgaaaaagaa tatggtcttg atacattgac aatgcacgca gatgctatta aacctggtca
    19081 acgtgtttta attgttgatg accttttagc tactggtgga acagtaaaag cgacaattga
    19141 aatgattgaa aaactcggtg gcgttgtcgc aggctgtgct ttcctagttg aactagatgg
    19201 attaaatggt cgtaaagcta ttgaaggtta tgatactaag gtattgatga actttccagg
    19261 ataaggccta ttcaagatag atgaatgata agggcgcctc taagtgctat gataatagtt
    19321 gagaggtgct ttttgttaca catttcataa ttttaggacg atagagagga tttgaaaggt
    19381 tatgacttat ttggaacaat atcagtcagg gcaattgact ctaccaagtg ctttattttt
    19441 tcactttaaa tctattttta agacagcaga cgatttttta gtttggcaat ttttctattt
    19501 acaaaataca actaatttat ccgatctaac accgagccgt attgcgacat cacttgataa
    19561 aacagtggca gatatcaatc gttctatatc gaatttaaca agtcaaggtt tattagatgt
    19621 caaaacaatt gagttgaatc atgaaatcga gattatcttt gatacaagcc ctgtgtttgc
    19681 taaattagat aaacttttcg aagaggataa tcaagtaata atagacaata aaacctctga
    19741 ttcgaatcgt ctgaaagatt tagtaggaga cttcgaacgt gaattaggtc gcttattaag
    19801 tccttttgaa ttagaagatt tacaaaagac actgcaagaa gatcagacag atcctgatat
    19861 tgttagggca gctcttagag aagcggtttt caatgggaaa acaagttgga attatatcaa
    19921 tgctatcctt cgcaattggc ggcgtgaagg attgacgaca cttcgacaaa ttgaagagag
    19981 aaaacaagca cgtgaagata accaaatgaa agatttagcg atttcggatg attttaaaaa
    20041 tgcaatgaat ttatgggaat aagtttacta ggaggagaca gaatggttga tacattaggt
    20101 ctgctccttt tcttttgatg ttaatgatgt tatactttta acagagaaaa actgaaggaa
    20161 atacaatgga tttacaatta tcaaaacgat tacaaaaagt tgcaaactac gttccaaaag
    20221 gagctcggtt attagatgta ggaagtgatc atgcttactt acctattttt ttgttgcaga
    20281 tgggatactg tgattttgcc attgctggtg aagtggtgaa tggaccgtat cagtctgcgt
    20341 tgaaaaacgt ttcagaacat ggtctcactt caaagataga tgttcgctta gcaaatggtt
    20401 tgtcagcttt tgaagaagca gataatattg atacgataac tatctgtgga atgggtggtc
    20461 gtttgattgc tgatattttg aataacgata tagacaaact tcagcacgtt aaaacactag
    20521 tcttacaacc taataatcgg gaagatgatt taagaaaatg gttagctgca aatgattttg
    20581 aaatagtagc agaagatatc ttgactgaaa atgacaaatg ctatgaaatt ttagttgtta
    20641 agcatggtca tatgaattta actgctaaag aattgcgatt tggtccattt ttattatcca
    20701 ataacacaac ggtttttaaa gaaaaatggc aaaacgagct taataaatta acttttgctt
    20761 taaactctat tcctaatagt aaaattgaag aacgcgctat tttggaagat aaaatacagg
    20821 atataaagga ggttcttgat gaaagctaga gaacttattg atgtatatga aacttattgt
    20881 ccacaggaat tatctatgga aggagatatt tcaggtcttc aaattggatc tttagataaa
    20941 gaaataaaga cagtcatggt agctttagat gttcgagaga ccacagtagc tgaagcaatt
    21001 gaacgtcaag tagatcttct gattgtaaag catgctccta tttttcgtcc attaaaggat
    21061 ttggtagcaa caccgcaaaa taagatttat atagaccttc ttaaatcaga cattgctgtt
    21121 tatgtgagcc atactaatat agatatcgtt cctaatggac ttaacgactg gttttgtgaa
    21181 ttattagata ttcaatatcc cgatatacta tctgagacaa gtaatggtta tggtattggt
    21241 cgcattggtg atgttagacc ccagagtttt gaattttttg catggaaaat taaagatgtg
    21301 tttggtttag atagcgttcg tttagtttca tatgataagt ctaatcctga aattcaacga
    21361 gttgctattt gcgggggtag tgggcaaagt ttctacaaag aagctattgc aaaaggagca
    21421 gatgtttttg taacgggaga tatttattat catactgcac aagaaatgat cacaaatggt
    21481 ttgcttgcga ttgacccagg tcatcatatt gaggttttat ttgtttctaa aatagctact
    21541 atgattgagc agtggaaact tgaaaaagac tgggatattt cagttttaga gagtaaagct
    21601 ccaacaaatc ccttttacca tatgtaggag ctattatgaa aaagatagca attattggtg
    21661 caggagcagt cggagcgaca ctagcctatt acttatcaaa agaaaaggat attcaggtta
    21721 ctgtttttga ttatggagtt ggacaagcaa ctaaggctgc tgcggggatt attagcccat
    21781 ggttttcaaa acgtcgtaat aaatcctggt atcgcatggc gaggttgggg gctgattttt
    21841 attcaaatct agtcacggat ttacaaaaag atggttttga aataaaattt tatcagcaaa
    21901 ctggtgtttt cttattaaaa aaggacgaga gtcagttaga gtcattgttt gcactagcag
    21961 ataaacgtcg actagaatct cctctaatag gcgatttaca aatactaaat aaaagtgaag
    22021 caaatactca ctttcctgag ttagatggct atgaacagtt actttatgcg agtggaggag
    22081 cacgagtaga aggggcggac ttaacacgaa tattgttaga agcaagtggt gttaatgtca
    22141 tcaaggatga agttcacttt acgataactg ataatggctt tcgtgttcaa ggaattgatt
    22201 ttgataaact tgttttagcg agtggagcct ggctagctaa gatattagat gagcacaact
    22261 atcaggtaga tgttcgccct caaaaagggc aattgcgaga ctattatttt agcaatatta
    22321 atacaggaaa atatcctgtt gtaatgccag aaggtgagct agatattatc ccatttgata
    22381 atggtaaagt ctctgtaggt gcaagtcacg aaaatgatat ggcttttgat ttaaacattg
    22441 attttaaagt cttagacaag tttgaagagc aagctattgg ctattttccg caattaaaaa
    22501 aagcagatac gacttctgaa cgtgttggaa ttcgtgccta tacaagtgat ttttctccat
    22561 tttttggccc tgtcccttgt atggagggtg cgtatgctgc aagtggatta ggatcaactg
    22621 gcttaacagt gggcccatta attggttatg aattatgtca attaatctta aataaagaaa
    22681 atcagctaaa tttagaggat tacgatatta cgaaatacgt gaaaaagatt agtagatgat
    22741 agcgtttaaa aaaagtgtta taatatgtag gattaatata aataaggaga ccttactatg
    22801 aaaggtatta ttttagcggg aggttcaggt actcgcttgt acccattaac tcgtgctgca
    22861 tcaaaacaat tgatgccgat ttatgataaa ccaatgattt attatccatt gtcagttttg
    22921 atgttggcag gaataaaaga aatactgatt atttctactc cacaagattt gcctcgcttt
    22981 gaagatatgc ttggagatgg ttcagaatta ggaatctctc tttcttatgc tgaacaacca
    23041 agcccagatg ggttagcaca ggccttcatt atcggtgaag aatttattgg cgatgatcac
    23101 gttgccttag tattaggtga taatatttac catggaccag gtttatctgt aatgctacaa
    23161 cgtgcagcta gtaaagaaag tggagcaacc gtctttggct atcaagtaaa agatccagaa
    23221 cgttttggtg ttgtcgagtt tgatactgat atgaatgcca tttctattga agaaaaacca
    23281 gcgcaaccta agtctaatta tgctgtcaca ggtctttatt tctatgataa cgatgtggtt
    23341 gaaatcgcta aaaatattaa gccaagccct cgtggagaac ttgaaattac tgatgttaac
    23401 aaagcctacc ttgatagagg tgacttatca gttgagttaa tgggacgtgg atttgcgtgg
    23461 ttagacactg gaactcacga aagccttctt gaagcagcac aatatattga aacagtacaa
    23521 cgcatgcaaa acgttcaagt tgctaacctt gaggaaattg cttaccgcat gggctacatc
    23581 actcgtgaac aagtccttga gttggcacaa ccacttaaga aaaatgagta tggtcaatac
    23641 ctacttcgtt tgattggaga agcctaatga ctgaacaatt ttttgataaa gaattaactt
    23701 gtcgtccaat taaagtcatt cctggattgt tggaattcga tattcccgtt cgtggagaca
    23761 accgtggatg gttcaaagag aactttcaaa aagaaaaaat gataccgctt ggtttcccag
    23821 aaagcttctt tgaggcagac aaactacaaa ataatatttc gtttaacaaa aaaaatactt
    23881 tgcgaggtct ccatgcagaa ccttgggata aatatgtttc gatcgctgat gaaggacgtg
    23941 tgatcggtac ttgggttgac ctccgtgaag gtgacagttt tggtaacgtt taccaaacga
    24001 ttatcgatgc ctcaaagggt atctttgttc cacgcggcgt tgctaatggt ttccaagttc
    24061 tttcagataa agcagcttat acttatctcg ttaacgatta ttgggcactt gaactcaaac
    24121 caaaatatgc tttcgttaac tatgcagatc caaatctagg cattcagtgg gaaaaccttg
    24181 aagaagcaga agtctcagaa gcagataaga atcacccact tctcaaagat gtaaaacctt
    24241 tgaagaagga agatttgtaa tgtttgatct ttttttgact atcaccaatg aattgaatct
    24301 aatagggatt acgcctttgt tgatgggatc ggttggctta gaagtacgta caggtcgaga
    24361 ttggaaagct agagacttag atattcatgt tcctagtgac cctagaggat gggaggcacc
    24421 tgatgagggg cgtattttcc aattcgctga aataaatgtg ataatggaaa aactaggatt
    24481 taatcttgta gatcgtcatg agcatgaata ccagaaagaa ggcctttctg ttgagtttgg
    24541 tggtctccac tcacttccag aatttgcagg tatacctata gatgacttgg aagaaattga
    24601 gacaaatggc gctcactatt ttctaccaac tttagatcaa tacctaaaaa tataccaagc
    24661 atcctcaaaa gattcttatc gatcagataa gaataatcac aaagactttg caaaaattga
    24721 ttatttagaa aaattattaa gtaataaaat ggttcgtaat gcgaaccact aaaaaggttt
    24781 gctctggata acagcaagtc ttaataaaaa attggagaaa aaaatgtata aaaacattat
    24841 cgtaactggt ggagctggtt tcatcggatc aaattttgta cactatgttt acaataacca
    24901 cccagatgtt catgtaactg tccttgataa attgacatat gcaggtaatc gtgctaacat
    24961 tgaagccatt cttggtgatc gtgttgagtt agttgttggt gacatcgctg acgctgaatt
    25021 ggtagacaaa ttggctgcta aagcggatgc tattgttcac tatgcggctg agagtcacaa
    25081 cgataactca ttggaagatc caagtccatt tatccataca aactttatcg gaacttacac
    25141 tttgcttgaa gcagctcgta aatacgatat ccgtttccac cacgtgtcaa ctgatgaagt
    25201 ttatggagat cttccacttc gtgaagacct tccaggaaat ggtgaaggac caggtgaaaa
    25261 attcactgct gaaacaaaat acaatccatc atcaccttac tcatcaacta aggcagcttc
    25321 tgaccttatc gttaaggcat gggtacgttc cttcagcgta aaagcgacca tttcgaactg
    25381 ttcaaataac tatggaccat accaacacat cgaaaaattc attccacgtc aaattacaaa
    25441 tatcttggca ggaatcaaac cgaaacttta tggtgaaggt aaaaacgtcc gtgactggat
    25501 ccacacaaat gatcattcaa caggtgtctg ggctattttg actaagggtc gtattggtga
    25561 aacatacctt attggtgccg acggtgagaa aaacaacaag gaggttcttg aacttatcct
    25621 tgagaaaatg ggtcaaccaa aagacgctta cgatcacgta actgaccgtg ctggtcatga
    25681 tcttcgttac gctatcgatt ctacaaaatt gcgtgaagaa ctcggttggg aaccacaatt
    25741 tacaaacttt tcagaaggtt tggaagaaac tattaaatgg tatacagaaa atgagacatg
    25801 gtggaaagca gaaaaagatg ctgtagaagc caagtatgct aaaactcaag aagtcattaa
    25861 gtaattgaac caaaaaacct atcattttga tgataggttt tttatgtacg actagttaga
    25921 tagctaattg gtctgttttt gtcatggttt gaggttggta atagacattt tgatagtcat
    25981 taccaacttt ttgtactagg taaagtcccg ctttattcat tttgaattga ttgttaatga
    26041 gactctcttg attatcgtgt ttgataacag cccaagtttg actgttttta tcatagataa
    26101 cttgttgttt tgaactattt tctagtaatt ctacttcttt gctgttggct aatttatcaa
    26161 aacttgtgcg atcaatgttt ggaaccatca tgtaaccata gctatcacct ttgttgtcat
    26221 gcttttggct tatagtgata aaaggattac taacgattga ggtattttta gaagtacgat
    26281 taatgctgtt ccaagtacct gtttgctctt tgcgttcaat atcaatagtg ctatttttaa
    26341 agaagatata accaatattg cgaccaggtt cttttgattc taaaaagaca ctctttgtat
    26401 ctgtaaattg ttgagaactt gcttgcttta aatcaacagt ttttccattg acgtatgtag
    26461 tataaggtgt tttagaatcg tcttttcgtt gatcaattgt tgtagaaaca tttctaatgc
    26521 cgttagtatt cttgatgttg ctacctaaaa agacaatctt atcatttaag ataacccaac
    26581 ctttttgtgc tgttaaggtg cgatcccagt tagtaaaatc catagcggca agagcaaagt
    26641 gattattaag tttgacggaa ccaacaaagt cagatgttcc tgtaacttga ccggtttttt
    26701 ctttagcgtc tttgctatgt ttgctcatga aatccttagt tgtatcttca cgcttagcat
    26761 ctttttcagt tgttccagcc attttataag gattgacggt tggccaaaaa tgattactat
    26821 aatgagattg atcactatta taaagataga acataccatc tccggtatac caaccacgtg
    26881 tattttcatc attcattcct tcatagttga gggtgcgttt agaatgtaat gaaagcgcga
    26941 aaccaaagtc tttctcggca ttataataag ctaagcggtc catgctatta aaggttgata
    27001 aattactttt taaaggttta gtagcgactg tactgtcatt aagcagctta ttcatgttgg
    27061 caatatcgga atacgatttg aggttattga agacgttgta gaatttattt gaagtgataa
    27121 tcgttttaat agttgatttg aggtctaagt ttcgctcttc attagacatg ttagccaatc
    27181 tgaggaaacc tctgagaact tcaactgcag ccgaatgcga agaagcagcc tctctactaa
    27241 ttgagcgtcc acgactcata tccattaact caccttttac aattaaaggt aaaaatgatt
    27301 gattaatcca tttataaacc atatcaagtt cttgattaga gattttatag tcagtttctt
    27361 gaatgatagg cagcaattgt gtcaaaccat ctatcagaac attaccatag gcgccagtat
    27421 aagcaacatt cgtatggtcg atgtaagaac cgtcagcata gaaaccttca gctttagtag
    27481 cagtagtaaa aagatttttt agagaatgag aagttttttc gataatagta ttgtctttac
    27541 gaagtaaacc ttcaatgatt ttaacgcgcc ccatatcgac tagattacca ccaagggctt
    27601 taaatggatt gtcaagcgtt ttacggaaat atcctgcatc aggaacaaag tgttcaattg
    27661 ggtcggtata agtttttatt tcagcgtcag tgaagtagtt attcatgaga gctagggtag
    27721 ctgtaattga gcgagggaca ccgatttcaa aatcccacca attggcagag ccttctatat
    27781 ctttattaac attgtagaag ttttgatgaa gccaagccag actctctttt acagtacgaa
    27841 tagctttttc atttttgtaa atagtagagt ggggattggt gatttgttta gctaaatctt
    27901 ccaaacgacg ataagtagcg gttaactgtg ctgaattatt gagattatct aaatctttcc
    27961 aaaggaaagt gtgattagaa tccagtttga tagttttgat gtttttggcg ttagtttcat
    28021 ctaatttctg attaatcttt tgcatgttcg aatcattagt atcgtaaaca tgattaccaa
    28081 tagtcacgtc gttccatttg tcgaggagtt ttgtaaactt atcttctgtt gaagctgtaa
    28141 ctgataacgg tacttcttta attattttac cagatttatc agatatttta acagttgttg
    28201 aaccagtagc gtttggatac aatattccac caacaatttt cccgagagac ggattggtta
    28261 gagtgtattg gtagtcagct ttattaaaaa cgtaattttt gtttaaagcc gtattaacgc
    28321 tttcttcaat ttgtgttgtg acgggttgtg gatgctctga gtctttaggg ccttttgctt
    28381 tcattgatat attatcaaaa gtagcagaac cagttccttt ttcataataa agttcaagtg
    28441 tcacctcgga gacatttaac tttgggttat atatctttgt taaggtatgt ttgttagtag
    28501 taccgctggt catctcagaa agccaaagtc gcgtattgtt gtcttttttc tccataatac
    28561 gaaggaaagc ttgtccagcc ttgttacttg tttcaatatc aaacttgacc tcatattttt
    28621 tagtgggatc aatgttcact ttttgtgtta cagcacctct aaagcctttg tcgctggata
    28681 ggcttacttg gccttgttcg gtacgttgga taataggtga tttatcagca gtattttctt
    28741 tatcaatcca agcagaccat tccctagcgc ttgtatgtga ccagcttcca gatacagggt
    28801 tagtttgatt aaaatctccg tttttcacca gctccttatt ttgagtttga ttatctacta
    28861 cttgtttcat tgtgctatga gtggctgttg cagttgcggt tgaagctgtg gggttagaca
    28921 gatcttgttg tgtggttgtg gtgctagtct ctttgacagt ggtactttca gtctggagaa
    28981 cttcaatatt attagtttga gtatctctta tttgggacgt tgaggtagtt ttggtaagct
    29041 cttcagcttt agcttggtaa ctattgttaa ccaatattgt tcctaaaata agggctgaat
    29101 aaagcataat acgatgtttc tttttgattt ccatttttac tccttagggt ttaaaattgt
    29161 aaactggttt ttcctaatta tagcatgctt taaaggttta taataaagaa cctttaaaat
    29221 gtcatcaaag tgaaaagtaa ataactggta aacaaaataa tttgtttaat catttgtgtg
    29281 ctactttgat agtaaattgc ctaaataaag gctatttttt gttataatat gagaatgaga
    29341 ggagtttgaa atgactaaat tagcaacaat ttgttacatt gacaatggca aggaattact
    29401 tctattgcac cgcaacaaaa aagagaatga tgttcatgag ggaaagtgga tctcggtagg
    29461 cggtaaactg gaggcaggtg aaactccgga tgaatgtgct aagcgtgaaa ttctagaaga
    29521 aacccattta acagtaaaaa aaatggattt taaaggagtc atcacttttc ctgaatttac
    29581 gccaggtcat gattggtata cctatgtctt taaagtaaca gattatgaag gagagttaat
    29641 ttcagatgat gaatcacgag aaggaacctt agaatgggta ccatatgatc aagttctctc
    29701 taagccaacg tggcaaggag attacgaaat ttttaaatgg attttagaag atgttccttt
    29761 cttttctgct aagtttgttt atgatgaaca ccaaaattta attgaaaaga cggttaattt
    29821 ttatgaaaaa tagagatttt agggttagag accagctcga aacattcgga gtcagagttt
    29881 ctgctttaat catagaaaat caaaaactgc tattaatata tgctcctcat ctcgataaat
    29941 actatttgcc aggtggggcg cttcaagttg gagaagatag caataaggct gttgcgagag
    30001 aagtacttga ggaaataggt cttcacagcc aagttggtga tttagcctat attgttgaga
    30061 atcagtttaa tattaagaga catcattatc actcagttga atttttatat tttgttaatc
    30121 tcttgggtca ggcgccagaa tctatcaaag aaggaacgca taagcgacat tttgtatggc
    30181 ttccaataaa agaattaaca aaaattgatt gtaatcctaa ttttttagca caggatttga
    30241 tagaatggcc aggtcatgtt gtgcataaaa tcgttcaaaa ttaacttttt acactcttgt
    30301 tttgag
//
LOCUS       DATRTN010000015        28620 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.015, whole genome shotgun sequence.
ACCESSION   DATRTN010000015 DATRTN010000000
VERSION     DATRTN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28620
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.015"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..142)
                     /locus_tag="VBE22_000733"
     CDS             complement(<1..142)
                     /locus_tag="VBE22_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEO7202205.1"
                     /translation="MKIDKRHLLNYSILIPYLILSILGLIVIYSTTSATLIQLGANPF
                     RSV"
     gene            complement(248..3043)
                     /gene="ppc"
                     /locus_tag="VBE22_000734"
     CDS             complement(248..3043)
                     /gene="ppc"
                     /locus_tag="VBE22_000734"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEO7202206.1"
                     /translation="MSHPKLESSSNKEIITEEVGLLKQLLDEATQKLIGSESFDKIEK
                     IVSLSSTDDYTGLKETISALSNEEMVIVSRYFSILPLLINISEDVDLAYEINYKNNLN
                     QDYLGKLSTTIDVVAGHENAKDILEHVNVVPVLTAHPTQVQRKTVLELTSKIHDLLRK
                     YRDVKAGIVNQEKWYADLRRYIGIIMQTDTIREKKLKVKNEITNVMEYYNRSLIKAVT
                     KLTAEYKALAAKKGIHLENPKPLTMGMWIGGDRDGNPFVTAETLRLSAMVQSEVIINH
                     YIEQLNELYRNMSLSISLTEVSPELVTLANQSQDNSVYRENEPYRKAFNFIQGKLVQT
                     LLNLKVGSYPKEKFVSRQESSDIVGRYIKSHVAQVASDIQTEELPAYATAEEFKQDLL
                     LVKQSLVQYGQDSLVDGELACLIQAVDIFGFYLATIDMRQDSSINEACVAELLKSANI
                     VDDYSSLSEEEKCQLLLKELTEDPRTLSSTHTPKSELLQKELAIFQTARELKDQLGED
                     IIKQHIISHTESVSDMFELAIMLKEVGLIDANQARIQIVPLFETIEDLDNSRDIMTQY
                     LHYGLVKKWIATNNNYQEIMLGYSDSNKDGGYLSSGWTLYKAQNELTKIGEENGIKIT
                     FFHGRGGTVGRGGGPSYEAITSQPFGSIKDRIRLTEQGEIIENKYGNQDAAYYNLEML
                     ISASIDRMVTRMITNPNEIDNFRETMDGIVAESNAVYRNLVFDNPYFYDYFFEASPIK
                     EVSSLNIGSRPAARKTITEISGLRAIPWVFSWSQNRIMFPGWYGVGSAFKHFIEQDEA
                     NLAKLQTMYQKWPFFNSLLSNVDMVLSKSNMNIALQYAQLAGSKEVRDVFNIILNEWQ
                     LTKDMILAIEQHDNLLEENPMLHASLDYRLPYFNVLNYVQIELIKRLRSNQLDEDYEK
                     LIHITINGIATGLRNSG"
     gene            3252..5051
                     /gene="pepF"
                     /locus_tag="VBE22_000735"
     CDS             3252..5051
                     /gene="pepF"
                     /locus_tag="VBE22_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO7202207.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALEDIKVFKKN
                     YEGKLNCVEDFTSALMEIEHIYIQMSHIDTYAFMPQTTDFSNEEFAQISQAGSDFATK
                     ANVLLSFFNTTLANADIKILDSLENNPHFKATIRQAKIQKQHLLSPEVEKALTNLNEV
                     LNTPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVTYENYFQNHENAEIRKKSFRSFSKG
                     LRKHQNAAAAAYLAKVKSEKLIADMRGYDSVFDYLLSEQEVDRSMFDRQIDLIMDEFG
                     PVAQRFLKHIADVNGIEKMTFADWKLDIDNELNPEVSINDAYDLVMKSVAPLGKEYSQ
                     EVERYQKERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSFFNTHMSTYYVEAPSTFNELLLSDYLENQFDTARQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGAEQLNAIMKEVLTQFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTTGYLNLKNNPNGAKEWLAFLKSGGSRTPLETALLIG
                     ADISTDKPLRDTINFLSNTVDQIINYSNLIEQG"
     gene            complement(5110..5499)
                     /locus_tag="VBE22_000736"
     CDS             complement(5110..5499)
                     /locus_tag="VBE22_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEO7202208.1"
                     /translation="MKKYIKLFLLTVFATTLVACGQPSTSNKTTTSSTLEVGKVELVV
                     KEDTNVLSEKVVYHKGDTVLDVLKANYKVKEKDGFITSIDGISQDETKGLYWMFKVNN
                     KLAPKAANQIKVKKNDKIEFYQEVYKK"
     gene            complement(5483..6007)
                     /locus_tag="VBE22_000737"
     CDS             complement(5483..6007)
                     /locus_tag="VBE22_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7202209.1"
                     /translation="MIKNSTLTKLTRVAILSALCVVLRYAFAPLPNIQPITAIFLITV
                     VLFDLKEGVATVTITMLVSSFLMGFGPWVFLQIISFALILCLWKFLIYPLTKAVCFGK
                     ITEVVLQTFFAGGLGVVYGVIIDTCFAWLYHMPWWTYVLAGLSFNMAHALSTCLFYPL
                     LLPILRRFRNEKIH"
     gene            6164..7012
                     /locus_tag="VBE22_000738"
     CDS             6164..7012
                     /locus_tag="VBE22_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEO7202210.1"
                     /translation="MRRRIKPIVVAVFFSLFGLLLIIGHLHSTNTLKKELVEAKKTIP
                     SVKASKVPQKSTSSKDKEFVLKPIIDVSGWQLPKEIDYDTLSKNISGVVIRVFGGSKI
                     SKTNNAAYTTGIDKSFKTHIKEFQKRNIPVAVYSYALGSSVKEMKEEAQIFYKNAAPY
                     KPTFYWIDVEEETMSNMNKGVQAFRKELKRLGAKNVGIYIGTYFMTEQGISVKGFDAV
                     WIPTYGSDSGYYEAAPQTELKYDLHQYTSQGYLPGFNQPLDLNQIAVNKDKKKTYEKL
                     FGKVKD"
     gene            complement(7045..7662)
                     /locus_tag="VBE22_000739"
     CDS             complement(7045..7662)
                     /locus_tag="VBE22_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEO7202211.1"
                     /translation="MEILIAGGSGFLGKQIIKAALTKGHKVAYLSRHEGKGDIFKDPR
                     LTYIRGDITEADKIHLEDRTFDILIDCIGAIKPNQLDELNVKATQKAVALCHKNQIPK
                     LVYISANSGYSAYIRSKRKAEQIIKASGLDYLFVRPGLMYGEERPLSIFQAKCIKLFS
                     HLPFLGIVVQKVFPTKVVIVAEAIVTTLRKKPTQKILSIEELNNK"
     gene            7957..8430
                     /locus_tag="VBE22_000740"
     CDS             7957..8430
                     /locus_tag="VBE22_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEO7202212.1"
                     /translation="MSKKNKIKKTLVDQILDKAKIEHDSLQLDALQGDLPNGIQKQDI
                     FKTLALIGDKTGPIIGILPLTEHLSEKKLAKISGNKKVQMIPQKDLQKITGYIHGANN
                     PIGIRQKHNYPIFIDTIALEKQELIVSAGEIGRSIRINSEVLADFVNAKFADIKE"
     gene            8452..9093
                     /locus_tag="VBE22_000741"
     CDS             8452..9093
                     /locus_tag="VBE22_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO7202213.1"
                     /translation="MKFYLVRHGKTQWNLEGRFQGANGDSPLLEEAIEELEELGQYLS
                     SIHFDAVYSSDLGRARDTVNILNDANSCPKEIHYTPQLREWALGTLEGCKIATMQAIY
                     PRQMTAFYQNPLQFKHDMFGAESLYQTTHRVESFLRSLASKNYDKVLIVGHGANLTAS
                     IRSLLGYQYGSLHYKDKLDNASLTIIETHDFKDFNCLTWNDKSYLRQEVKMTH"
     gene            complement(9129..10031)
                     /locus_tag="VBE22_000742"
     CDS             complement(9129..10031)
                     /locus_tag="VBE22_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7202214.1"
                     /translation="MITSIVFDVDDTIYDQQAPYRIAVEKCFPDFDMSAINQAYIRFR
                     HYSDIGFPRVMAGEWTTEYFRFWRCKETLLEFGYREINEATGIYFQEIYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVKKLGLYDYVDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPSTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRSLKPGIKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKSNPVLEMGLNNGLMMAAERLLESNMS
                     VDKVVILLRLTAKQEKVLRMKYAR"
     gene            10206..11696
                     /gene="lysS"
                     /locus_tag="VBE22_000743"
     CDS             10206..11696
                     /gene="lysS"
                     /locus_tag="VBE22_000743"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEO7202215.1"
                     /translation="MSNQHIEELNDQQIVRREKMAALTEQGIDPFGKRFERTATSGQL
                     NEKYADKSKEDLHDIEETATIAGRLMTKRGKGKVGFAHIQDREGQIQIYVRKDSVGEE
                     NYEIFKKADLGDFLGVEGQVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDIETI
                     YRKRHLDLISNRDSFDRFVTRSKIISEIRRFMDSNGFLEVETPVLHNEAGGASARPFI
                     THHNAQDIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEAYQ
                     AYADYQDIMDLTEGIIQHVTKTVKGDGPINYQGTEIKINEPFKRVHMVDAVKEITGID
                     FWKEMTLEEAQALAQEKNVPLEKHFTTVGHIINAFFEEFVEDTLIQPTFVFGHPVEVS
                     PLAKKNDTDPRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQASAKELGDDEATG
                     VDYDYVEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(11771..12241)
                     /locus_tag="VBE22_000744"
     CDS             complement(11771..12241)
                     /locus_tag="VBE22_000744"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEO7202216.1"
                     /translation="MTIIEGQLVANEMKIGIVVSRFNELITSKLLSGAVDGLLRHGVS
                     EEDIDIVWVPGAFEIPYMARKMALYKDYDAIICLGVVIKGSTDHYDYVCNEVTKGIGH
                     LNSQSDIPHIFGVLTTDNIEQAIERAGTKAGNKGYDCALSAIEMVNLDKKLRER"
     gene            complement(12256..13449)
                     /locus_tag="VBE22_000745"
     CDS             complement(12256..13449)
                     /locus_tag="VBE22_000745"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEO7202217.1"
                     /translation="MAFSPIKKLLQDIKSGKMVVLMDDENRENEGDLICAAEMVTKES
                     INFMAKFGKGLICLPLSNYYAEKLELAQMASHNTDNHETAFTISIDHLSTSTGISAED
                     RALTAKMVANDSSKAKDFRRPGHLFPLLAKEGGVLARNGHTEATVDLCRLAGLKECGL
                     CCEIMAEDGSMMRKDELLAFAQKHDLAIATIKQLQDYRRQEEGGVVREIEIQLPTQFG
                     HFTAYGYSEVVASKEHVALVKGDISSGEDVLCRLHSECLTGDVFHSLRCDCGEQLANA
                     LQQIEAEGRGVLLYMRQEGRGIGLINKLKAYYLQEEGLDTLEANLALGFEGDERDYGV
                     SAQLLKDLGINSINLLTNNPDKIQQLEAEGICVKNRVPLQVAATAYDLNYLKTKKEKM
                     GHLLD"
     gene            complement(13467..14117)
                     /locus_tag="VBE22_000746"
     CDS             complement(13467..14117)
                     /locus_tag="VBE22_000746"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEO7202218.1"
                     /translation="MFTGIIEEMGQVSRIRNGIKSQQLSIDAPKLVPLLRKGDSVAVN
                     GVCLTVLDKSEKAFIADVMPESMMRTSLAALRLHSKVNLELALRSDSRLGGHFVLGHV
                     DGVGKIEKIQKDDIAVRFSIDAPPSIMSYIIEKGSVALDGISLTVVSFTEHSFEVSVI
                     PHTMTQTNLSLKKVGDLLNIEVDVLGKYAEKFLAPTNRTNHTSSVMDWSFLSENGY"
     gene            complement(14098..15207)
                     /gene="ribD"
                     /locus_tag="VBE22_000747"
     CDS             complement(14098..15207)
                     /gene="ribD"
                     /locus_tag="VBE22_000747"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEO7202219.1"
                     /translation="MMKKEDYMALALKEAEKGMGFVAPNPLVGAVIVKDDRIISKGYH
                     KRFGDLHAERQAIKNADEDISGSTLYVTLEPCCHVGKQPPCTEALIKSGIKKVVVGSL
                     DPNPLVSGKGIALLRKEGLNVEVGILREECDALNERFIFHMTYKQPFVYLKYAMTLDG
                     KIATKTGDSKWISNEHSRQSVQKLRQKCSAIMVGINTVLADNPRLTCRIPKGEALVRI
                     VCDSQLKIPLDSYLVKSAKTIPTWIATCSDNLAQQQTLKEMGCRLIKVPRKDGKLDLK
                     VLMTILGQDGIDSLLIEGGSSLHFSALKAGIVNRLIVFIAPKIIGGLKAKTAISGEGL
                     DWLNQAFRVKDIELSRMDSDVVIEGKVEHYVYRNY"
     regulatory      complement(15401..15515)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            15935..17275
                     /locus_tag="VBE22_000748"
     CDS             15935..17275
                     /locus_tag="VBE22_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001073016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO7202220.1"
                     /translation="MNQNHSVKRSLSEVNQSVEVPHNSSFWNTLRAFLGPGALVAVGY
                     MDPGNWITSVIGGATYRYLLLFVVLVSSLMAMQLQQMAGKLGIVTRQDLAQATASRLP
                     KPLRYLLFIIIELALIATDLAEVIGSAIALHLLFGWPLLLSIMITILDVFLLLLLMKL
                     GVQKIEAFVSVLILTILIIFTYLVVLSQPDLDAMFKGFLPHHELFNISHEGKNSPLTL
                     ALGIIGATVMPHNLYLHSSLSQTRRVDYHNKSSIKKAVRFMTLDSNIQLSLAFVVNSL
                     LLVLGASLFYGHANDISAFSQMYLALSDKTITGAVASSFLSTLFAVALLASGQNSTIT
                     GTLTGQIVMEGFLHFKLPQWLIRLCTRLLTLLPIFVIALLVGGEENTLDQLIVYSQVF
                     LSLALPFSIFPLIYFTSQKSIMGEHANAKWNTYLAYLVAIILTLLNLKLIMDLL"
     gene            complement(17376..18173)
                     /locus_tag="VBE22_000749"
     CDS             complement(17376..18173)
                     /locus_tag="VBE22_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202221.1"
                     /translation="MIRYSFKSKMLLLVFILTIIVNYLSATGLLTGNSQKSLSDRYQT
                     LLTPAPLAFSIWSVIYLLTFLVILRAIFSKSQSYQDNFASIFPYFLGLLLVNNIWTVF
                     FTSNLIGLSTIIIFAYCILLVIIIKILSKNKSKLLLRITFGIHAGWLLVASLVNLAVY
                     LVKIDFNYPLPKVYIAIIALIFITVLSLYLARVLQNAYLILSVFWAWLMVFKAHLEGN
                     FQHLYFSIQILSLIAAIIFGVSFFIIGYYNVKERYKQKNTSPYSRVL"
     gene            complement(18297..18509)
                     /locus_tag="VBE22_000750"
     CDS             complement(18297..18509)
                     /locus_tag="VBE22_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEO7202222.1"
                     /translation="MSEFTFEIVEKLLVLSENEKGWTKELNRVSFNGAPAKFDLRTWS
                     PDHTKMGKGITLSNEEFKVILDAFRK"
     gene            complement(18636..19922)
                     /locus_tag="VBE22_000751"
     CDS             complement(18636..19922)
                     /locus_tag="VBE22_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO7202223.1"
                     /translation="MSNVKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGINYAHARDAKVYVAANMVTHEGNELGAGPWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKEMGLTRVVLAREVTMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGQERQSL
                     KGEIPEPFSMSAVDMCMIEHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFEAIKEDLIDELWKVAQRELATGFYYHTPTENEQLFGARRKIPQYKFVGEVV
                     SFDNAKMEATIRQRNVIMEGDRVEFYGPGFRHFECFIEGLRDAEGNKIDRAPNPMELL
                     TITLPNPVKKGDMIRACKEGLVNLYQNDGTSKTIRA"
     gene            complement(20052..20978)
                     /locus_tag="VBE22_000752"
     CDS             complement(20052..20978)
                     /locus_tag="VBE22_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO7202224.1"
                     /translation="MEKIILTATAESIEQVKQLLAIGIDRIYVGEENYGLRLPHSFSD
                     DELREIAKLVHDAGKELTVACNALMHQEMMDNIKPFLELMKEINVDYLVVGDAGVFYI
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVETVLAREIPSEELFKMSENLEFPAE
                     ILVYGASVIHHSKRPLLQNYYNFTHITDEKTRERGLFLAEPGDPESHYSIYEDKHGTH
                     IFINNDIDMMTKVTELVEHHFTHWKLDGIYCPGDNFVAIAEIFVETARLIENGTFTQD
                     QAFLFDERIRKLHPKGRGLDTGFYEFDPSTVK"
     gene            21319..21594
                     /locus_tag="VBE22_000753"
     CDS             21319..21594
                     /locus_tag="VBE22_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEO7202225.1"
                     /translation="MSSPLKQEQTHESKTEQAQPKFQVFQEFDHSAKLRELVFFARIA
                     LFSITTVLISFLLLALSLTPVWSFLLAMLASLAVTSAVSSFIWSLRQ"
     gene            complement(21635..22066)
                     /locus_tag="VBE22_000754"
     CDS             complement(21635..22066)
                     /locus_tag="VBE22_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO7202226.1"
                     /translation="MSKLFKTLVISAASGAAAAYFLTTKKGKELRKNAEKFYGEYKEN
                     PEEYHQIAKDKASEYSNLAVDTFKDYKGKFESGELTTEDIVSAVKEKSGEVVDFANDF
                     VNQAKSKFSDEDTAKKENKAPETKVEDIVIDYKENTEDKEK"
     gene            complement(22063..22461)
                     /locus_tag="VBE22_000755"
     CDS             complement(22063..22461)
                     /locus_tag="VBE22_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEO7202227.1"
                     /translation="MIEIAVLIIAIAFVVLVLGILFVLKKVSETIEETKQTIKVLTSD
                     VNVTLYQTNEILAKANVLVDDVNGKVSTIDPLFVAIADLSESVSDLNLQARHIGQKAS
                     SATSSVTKAGSALAIGKAASKIFRKKGDKK"
     gene            complement(22476..23249)
                     /locus_tag="VBE22_000756"
     CDS             complement(22476..23249)
                     /locus_tag="VBE22_000756"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEO7202228.1"
                     /translation="MINPVAIQLGPFSIRWYAICIVSGMLLAVYLAMKEAPRKNIKSD
                     DILDFILMAFPLSIVGARIYYVIFEWAYYSKHPVEIIAIWNGGIAIYGGLITGAILLV
                     IFSYRRLINPIDFLDIAAPGVMIAQAIGRWGNFINQEAYGRAVKNLNYLPNFIKNQMY
                     IDGAYRVPTFLYESLWNLLGFVIIMSIRHRPRTLKQGEVACFYLVWYGCGRFIIEGMR
                     TDSLYLAGLRVSQWLSVILVIIGIVMIIYRRREQHISYY"
     gene            complement(23242..24177)
                     /locus_tag="VBE22_000757"
     CDS             complement(23242..24177)
                     /locus_tag="VBE22_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEO7202229.1"
                     /translation="MAVTVQMLVDRLKLNVIYGDEHLLSKRITTADISRPGLEMTGYF
                     DYYAPERLQLVGMKEWSYLMAMTGHNRYQVLREMFQKETPAIVVARDLEIPEEMYEAA
                     KDTGIAILQSKAPTSRLSGEVSWYLDSCLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSQVQLAIYLENFETGKVFDRLGNGNEEIELSGVKVPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKQMGFDATQTFEDRLTHLISQNEVNDD"
     gene            complement(24405..24668)
                     /locus_tag="VBE22_000758"
     CDS             complement(24405..24668)
                     /locus_tag="VBE22_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEO7202230.1"
                     /translation="MKSSFYKQRKGKLVCGVVAGLADKYNWDLALSRVLIALILYFTK
                     FGLLLYILLAVFLPYKEDIIETRRQGPRRRKDAEPVDDDGWFW"
     gene            complement(24749..25207)
                     /locus_tag="VBE22_000759"
     CDS             complement(24749..25207)
                     /locus_tag="VBE22_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEO7202231.1"
                     /translation="MDLVLSSQTDLTNYVKKVSIEDFGCQFLHTAHWNNRLRTTGGRF
                     FPNDRHLDFNPKIYREFGIDIFRKIVRHELCHYHLYIQGKGYQHRDKAFKELLEKVDG
                     LRYTPRVTAVKVNYHHYSCQSCGQVYRRRRRVNLRKFACGYCHGRLIESF"
     gene            complement(25170..27332)
                     /locus_tag="VBE22_000760"
     CDS             complement(25170..27332)
                     /locus_tag="VBE22_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEO7202232.1"
                     /translation="MENLNITQIAIDLGIKASQIEKVLELTDEGNTIPFIARYRKEMT
                     GNLDEVQIKSIIDLDKSMTALSDRKTTVLAKIEEQGKLTQELKKAIEEATKLADVEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNKDNLEEEAQNYLTDGFETTTKALSGAVD
                     ILIEAFSEDNKLRSWTYNEIWNYSSITAVVKDESLDEKQVFKIYYDFSEKISKLHGYQ
                     VLALNRGEKMGVLKVNFEHNLEKMFRFFAVRFKETSQYIDDLIVQTVKKKIVPAMERR
                     IRTELSEGAEDGAISLFSENLRNLLLVSPLKGKMVLGFDPAFRTGAKLAVVDQTGKLM
                     TTQVIYPVPPANQAKIEQSKIELAKLIKEFNIEIIAIGNGTASRESEAFVAEVLQDFP
                     DVSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLAENLDFVVETVVNQVGVNVNTASPALLAHVSGLNKTISENIVKYR
                     EENGQIKSRAEIKKVPRLGAKAFEQAAGFLRIPNAKNFLDNTGVHPESYEAVKKLLDQ
                     LTIKELDDFAKEKLQNLDLIATAESIGVGQETLKDIIEDLLKPGRDLRDDFEAPVLRH
                     DVLDVSDLKVGQELQGTVRNVVDFGAFVDIGVHEDGLIHQSRLIKRKRDKKTRKMPPL
                     PHPSKYLSVGDIVTVWVVEVDAERSRIGLSLIKPDGLD"
     gene            complement(27436..28479)
                     /locus_tag="VBE22_000761"
     CDS             complement(27436..28479)
                     /locus_tag="VBE22_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEO7202233.1"
                     /translation="MIELGITTFGETTILEETNQSYSHPERIRQLVAEIELADQVGLD
                     VYGIGEHHREDFAVSAPEIVLAAGAVRTNNIRLSSAVTILSSNDPIRVYQQFSTIDAL
                     SNGRAEIMAGRGSFIESFPLFGYDLADYDDLFNEKMDMLLAINSATNLDWKGHLTQTV
                     NERPIYPRALQRQLPIWVATGGNVDSTIRIAEQGLPIVYATIGGNPKAFRQLVHIYKE
                     VGSRNGHKPEQLKVAAHSWGWIEEDNQTAIDRYFFPTKQTVDNIAKGRPHWSEMTKEQ
                     YLRSVGPEGAIFVGSPEVVAHKIIGLVEALELDRFMLHLPVGSMPHKDVLNAIKLYGE
                     EVAPIVRKYFEKN"
BASE COUNT         9196 a         5617 c         4405 g         9402 t
ORIGIN      
        1 ttactgaacg aaaagggtta gctccaagct gaattagagt tgcactagtt gtcgaataaa
       61 ttacaattaa gcctagaata gacaatatca aatagggaat gaggatggaa taattcagaa
      121 gatgcctttt atcaattttc atattgtatt atttccttta atatatcaaa aaattaccat
      181 tatgcattat atcatctttc gaagtaaagt aaaaggaaga atagcaatga tcctccctct
      241 atcaatatta acctgaattt cgtaaacctg ttgcaatacc attaatagta atatgaatca
      301 acttctcata gtcttcatct agttggttac tacgaagacg tttaatcaat tcaatttgaa
      361 cataattcaa aacattgaaa tatggcaaac gataatctaa acttgcatgt aacattgggt
      421 tttcttctaa taaattatca tgctgttcaa tagctaaaat catatcttta gttaactgcc
      481 attcattaag gatgatatta aaaacatctc gaacttcttt acttccagct aactgagcat
      541 actgaagcgc aatattcata tttgattttg ataaaaccat atctacatta gataaaagag
      601 aattaaagaa tggccatttt tgatacatgg tttgtaattt tgctaagttt gcctcatctt
      661 gttcaataaa gtgcttgaag gctgacccaa caccatacca acccggaaac ataatacgat
      721 tttgtgacca tgaaaatacc catggaattg cacgtaaacc cgaaatttct gtaattgttt
      781 tacgtgctgc tggccgagaa ccaatattta aacttgatac ctctttaatt ggacttgctt
      841 caaagaaata gtcatagaaa taaggattat caaaaactaa atttctataa acagcattac
      901 tttcagccac aataccatcc attgtctcac ggaaattatc aatttcatta gggttagtaa
      961 tcatacgtgt taccatgcgg tctatggaag ctgaaataag catttcaagg ttataatagg
     1021 ctgcatcttg attaccatat ttattctcaa tgatttcacc ttgctctgtt aggcgaattc
     1081 gatctttgat tgaaccaaaa ggttgtgatg tgatggcttc ataagatgga ccacctccac
     1141 gaccaacagt cccaccacga ccatggaaaa atgtgatttt aataccgttt tcttcaccta
     1201 tttttgttag ctcattttga gctttatata gagtccaacc agatgataaa tagccaccat
     1261 ctttattact atctgaataa cctagcatta tttcttgata attgttgtta gtagcaatcc
     1321 attttttaac taatccataa tgtagatact gggtcataat atcacgtgaa ttatccaaat
     1381 cttcgatggt ttcaaataac ggtacaatct gaatgcgagc ttgattggca tcaattagtc
     1441 caacttcttt taacatgata gccaactcaa acatatccga aacgctctct gtgtgagaaa
     1501 tgatatgctg ttttataatg tcttcaccca attgatcttt taactcacgt gctgtttgga
     1561 aaatagccaa ttctttttga agtaactcgg atttaggagt atgcgtagaa gataatgttc
     1621 taggatcttc tgttaattct tttaagagaa gttgacattt ttcttcttct gaaagagaac
     1681 tgtaatcatc tacaatattg gcagacttca ataattctgc tacacaagct tcattaatac
     1741 tagaatcttg acgcatatca atagtagcta aataaaatcc aaagatatct actgcttgaa
     1801 taagacaagc taattcgcca tcgacaagag aatcttgtcc atattgaact aaagattgct
     1861 tcacaagtaa caaatcttgt ttaaactcct cagcagtagc gtatgctggt agttcttctg
     1921 tctgtatgtc agaagctact tgagcgacat gtgattttat ataccttcct acaatatcag
     1981 aagattcttg acgactaaca aatttctctt ttgggtaaga accaactttt aaatttagta
     2041 gggtttgtac tagcttacct tggataaaat tgaaagcttt acggtaaggt tcattttccc
     2101 tatataccga attatcttgt gactggtttg ccaatgtaac aagttcggga gagacttcag
     2161 ttagacttat tgaaagtgac atattacgat aaagctcgtt tagctgctca atataatggt
     2221 taataatcac ttcactttgt accatagctg ataatcttag tgtctcagca gttacaaagg
     2281 gattgccatc tctatcacca ccaatccaca tccccatagt cagaggctta ggattctcta
     2341 agtgaatacc ttttttagct gccagtgcct tatattctgc tgttaatttg gtaactgctt
     2401 taataagaga acggttgtaa tattccatta cattggttat ctcatttttg actttcaatt
     2461 ttttctcgcg aatagtgtct gtttgcatga taatgccgat gtaacgacgc aagtcagcat
     2521 accatttttc ttgattcacg attcctgcct tgacatctct gtatttacgt aataaatcat
     2581 gaatcttaga cgttaattcg agcaccgttt tacgctgcac ctgagtagga tgagctgtta
     2641 gcacaggcac aacattgacg tgttcaagaa tatctttagc attttcatgg ccagctacaa
     2701 catcaatagt cgttgataac tttcctaggt aatcttggtt aagattattt ttataattaa
     2761 tttcatatgc taaatcaacg tcttctgaaa tattaattaa gagcggcaaa atagagaagt
     2821 aacgagaaac aataaccatc tcctcgttgc ttaaagccga aatagtttcc ttcagaccag
     2881 tataatcgtc agttgatgat aatgaaacta ttttttcaat cttgtcaaat gattcactac
     2941 caatcaattt ttgcgttgct tcatccaaaa gctgcttcag taaaccgacc tcttcggtaa
     3001 tgatttcttt attgctacta ctttctaatt tcgggtggga caaaccatca cttcctctct
     3061 taaatctcac tactatgata ccattttaaa gtaaaaagta aaccattcta ggatttttag
     3121 atagaatttt cagattttta cttatttatg acagtaatca gtttttcttg ttcgactttt
     3181 ttgatatact gaggataatt gagattaact gattaattgt taattaacaa ctattattaa
     3241 aggagtatcg catggaactt aaaaaacgct ctgaattccc tgaaaatgaa ctttgggatc
     3301 tgactgctct ctacaaagat cgtcaagatt ttttactggc tattgaaaaa gctcttgagg
     3361 atatcaaggt tttcaaaaaa aattacgagg gaaaacttaa ttgtgttgag gattttacta
     3421 gtgcccttat ggaaatcgag catatttata tccagatgag tcacattgat acctatgctt
     3481 ttatgcctca aactactgac tttagcaacg aagagtttgc acaaatttct caagcaggat
     3541 cagacttcgc aactaaagct aatgtactac tttctttctt taatactacc ctagcaaatg
     3601 cagatataaa gattttagat tctttagaaa ataaccctca tttcaaagca actattcgcc
     3661 aagctaaaat tcaaaagcaa cacttattaa gtccagaagt cgaaaaagct ttgacaaact
     3721 taaatgaagt cctaaataca ccatatgata tttatactaa aatgcgtgct ggcgattttg
     3781 atatggaaga ttttgaagtt gacggaaaaa cctacaaaaa tagttttgta acttatgaaa
     3841 attactttca aaatcacgaa aatgcagaga tacgtaaaaa atccttccgt tcattctcga
     3901 aaggtctacg taagcaccaa aatgctgcag ctgcggctta tcttgcaaaa gtcaaatctg
     3961 aaaagttaat tgctgatatg cgtggttacg atagtgtttt tgattatctt ctttccgaac
     4021 aagaagttga ccgttcaatg tttgacagac aaattgatct tatcatggat gaatttggtc
     4081 cagtagctca acgattcctc aaacatatcg ccgatgttaa tggtattgaa aagatgactt
     4141 ttgcagattg gaaattagac attgataacg aacttaatcc cgaagtaagt atcaatgatg
     4201 cttatgactt agtaatgaaa tcagttgctc ctttaggaaa agaatattcc caagaggttg
     4261 agcgctatca aaaagagcgt tgggttgatt ttgcagctaa cgctaataaa gattctggag
     4321 gatatgctgc ggatccttac aaagttcatc cctatgtact catgagctgg acggggcgta
     4381 tgagcgatgt ctacactctt attcatgaga ttggacattc tggtcaattt atcttctcag
     4441 ataatcacca aagttttttc aatactcaca tgtccactta ctatgttgaa gctccatcta
     4501 cttttaacga acttttactt agtgactact tagaaaacca atttgataca gcacgtcaaa
     4561 aacgctttgc tcttgcacat cgcttaactg atacttactt ccacaatttc attactcact
     4621 tacttgaagc tgctttccaa cgtaaagttt acactctaat tgaggaggga ggaacttttg
     4681 gagcagaaca attaaatgct attatgaagg aagtattaac acaattctgg ggagatgcta
     4741 ttgaaataga tgacgatgct gctttgacat ggatgagaca agctcattac tacatggggc
     4801 tctatagcta tacttactct gctggtttag taatctcgac aaccggctat ctcaatctca
     4861 aaaataatcc aaacggagca aaagagtggt tagcattctt gaaatctggt gggagccgta
     4921 cccctttaga aacagctcta ttaatcggtg ctgatatttc tactgataag cctctacgcg
     4981 atactattaa tttcttatct aacactgtag accaaattat taattacagt aatcttattg
     5041 agcaaggcta aactacagcc tttcaaaaac ataaaaacaa gttggagata tctccaactt
     5101 gtttttatct tattttttgt aaacttcctg ataaaattca attttgtcat tctttttaac
     5161 tttaatttgg tttgctgcct taggagctaa tttattattg accttgaaca tccaataaag
     5221 ccctttggtt tcatcttggc tgatgccatc aattgaagta atgaaaccat ctttttcttt
     5281 aactttatag ttagctttaa gcacatccaa aacagtatca cctttatggt aaacaacctt
     5341 ttcacttaac acgttcgtat cttctttaac caccaattca accttaccaa cttctaacgt
     5401 tgagctagtt gttgtcttat tcgatgtaga tggttgcccg caagctacta aagttgtagc
     5461 gaatactgtt agcaagaata atttaatgta ttttttcatt cctaaatctc cttaaaatcg
     5521 gtaacaaaag aggataaaat aaacaagttg ataaagcgtg tgccatatta aaactaagtc
     5581 ctgctaatac ataagtccac caaggcatat gatataacca agcaaaacag gtatcaataa
     5641 tgacgccata aacaacccct aaccctcctg caaagaaagt ttgaagtact acctccgtta
     5701 ttttaccaaa acatacagct tttgtcagcg gatatatcag aaatttccac aagcataaaa
     5761 ttaaagcgaa ggaaatgatt tgcaaaaaga cccatggacc aaagcccatt aaaaaagaag
     5821 aaactagcat tgttatagtt acagtagcta caccttcttt taaatcgaat aagacaactg
     5881 taatcaaaaa aatggcagta attggctgaa tgttaggcag aggcgcaaaa gcatatctca
     5941 acacaacaca caaagcagat aatatagcga ctctagtcaa tttagttaaa gttgaattct
     6001 ttatcatgtc tttaattata cctcaaaact atgactaccg cttaaaagtc ttttttatta
     6061 caatttcatt tctaatgtta cataaacgac ctcttttttt aaaagaacgt aaataagcct
     6121 gtaaactttg attttttaaa ccttttaagt tacaatatca ataatgagaa gaagaattaa
     6181 acctattgtt gttgcggttt ttttcagttt atttggactt ttattaatta ttggtcattt
     6241 acattcgaca aatactttga aaaaagaatt agttgaagct aaaaagacaa ttccatccgt
     6301 aaaagcttca aaagtaccgc aaaaatcaac atcatcgaaa gataaagagt ttgttcttaa
     6361 accgattatc gatgtctctg gttggcaact tcctaaggag attgattacg atacgctttc
     6421 aaaaaatatt tcaggtgttg ttattcgtgt ctttggtgga tcaaagatat ctaagactaa
     6481 taacgctgct tatacaactg gaatcgataa atcgtttaag acccatatca aagaatttca
     6541 aaagcgaaat atcccagtag ctgtctacag ttatgcactt ggttcaagtg ttaaagaaat
     6601 gaaagaagag gctcagatat tttataagaa tgcagctcct tacaaaccaa ctttttattg
     6661 gattgacgta gaagaggaga caatgtctaa catgaataaa ggtgtccaag cattccgaaa
     6721 agaattaaaa agacttggtg ctaaaaatgt tggtatctac attggtactt actttatgac
     6781 tgagcaaggc atctctgtaa aaggatttga cgctgtttgg attccaactt atggtagcga
     6841 ttctggatac tatgaagcag ctccgcaaac tgaacttaaa tacgatttac accaatacac
     6901 ctctcaaggt tatctaccag gattcaatca accgcttgat ttaaatcaaa ttgcagttaa
     6961 taaagacaag aagaaaactt atgagaaact ttttggaaaa gtaaaagatt aaaagctact
     7021 gttaacagta gcttttttga taaactattt attatttaat tcttcaatag aaaggatttt
     7081 ttgggttggt tttttcctaa gcgtagtaac gattgcttct gccactatca caaccttagt
     7141 tggaaagacc ttttgtacaa caatacctaa gaaaggcaaa tgactaaata attttataca
     7201 cttggcttgg aaaatcgaga gaggtcgctc ttcaccatac atcaaacctg gtcttacaaa
     7261 aaggtaatcc agaccgcttg ctttgattat ctgctctgcc ttccttttac ttctaatgta
     7321 agctgaatag ccgctgttgg ctgaaatgta aactagcttt ggtatttgat ttttgtgaca
     7381 gagtgctact gctttttggg ttgctttaac gttaagctca tctagttgat tgggcttaat
     7441 cgctccaata cagtcaatta atatatcaaa agttctgtct tctaaatgaa tcttatcagc
     7501 ttctgtaata tctcccctaa tgtaggttaa tctaggatcc ttaaatatat cacctttacc
     7561 ttcatgtctt gataagtaag ccactttatg cccttttgta agcgctgctt ttattatctg
     7621 ctttcctaaa aaaccactac cacctgcaat cagtatttcc atagttccct ctccttcatt
     7681 ttgtaaattt agtatagaaa tttttatatg ccttgtcaaa gtaatcgttt aatacctgac
     7741 cacggtatga tttcaagtca ttacatcagg acacttcata tttagctatt tataaaactt
     7801 ctaatttaac attccaaata gtactgttgc gatatttatt atcattattc cctgaaatgg
     7861 tattcaagct tatttctaag agtcagtaga aatttactat aatcatcttt aaatgttata
     7921 ataaatatga aacaaactta ttagttaagg gtcattatga gtaaaaagaa taaaatcaaa
     7981 aaaactttag ttgatcaaat tttagataaa gcaaaaatcg aacatgatag tctacaattg
     8041 gatgctttac agggagattt acccaacggt attcaaaaac aagatatctt taagacatta
     8101 gcgctaatcg gtgataaaac cggtcccata ataggtatct tacctctcac cgagcattta
     8161 tccgaaaaaa agttagctaa aatttcagga aacaaaaagg tacaaatgat acctcaaaaa
     8221 gatctccaga aaataaccgg ttatattcac ggagcaaata atccaattgg aattcgccaa
     8281 aaacataatt accctatttt tatcgatact atcgctttag aaaaacaaga actcattgtc
     8341 tctgcaggag aaattggacg ttctattcgt attaatagtg aggttcttgc agatttcgta
     8401 aatgctaaat ttgccgatat aaaggaataa taattaggat tggagaaaaa tatgaaattt
     8461 tatcttgtta gacacggaaa aacacaatgg aatttagagg gacgtttcca aggtgctaac
     8521 ggagactcac ctctccttga agaagctatt gaagaattag aagaacttgg acaataccta
     8581 tcatctattc attttgatgc tgtttattct agcgacttag gtcgtgctag agatactgtt
     8641 aatatcctca atgatgctaa ttcatgccca aaagaaatcc actatactcc tcaacttcgt
     8701 gaatgggcat tgggaacttt agaaggttgt aaaattgcaa ctatgcaagc tatctatcct
     8761 cgtcaaatga ccgcatttta tcaaaatccc ttacaattta aacatgacat gttcggtgca
     8821 gaatctcttt atcagacaac gcatcgtgta gagagtttct tacgttcttt agcttcaaaa
     8881 aattacgaca aggtccttat tgtaggacat ggagcaaacc taactgcgtc tattcgttcc
     8941 ttactgggtt atcaatatgg tagtttacac tacaaagata aacttgataa tgccagcctc
     9001 acaatcatag aaactcatga ttttaaagac ttcaactgtc tgacttggaa cgataaatct
     9061 tatttaaggc aagaggtcaa aatgacacac taaaataatc cactagattc atctagtgga
     9121 ttatttaatt atctagcata cttcatgcgt aatacttttt cttgttttgc agttaggcgc
     9181 agtaaaataa caactttgtc aacgctcata ttactctcaa gcagacgctc tgctgccatc
     9241 attaaaccat tattaagtcc catttcaagg actggattac ttttatcgtt gatatcaaaa
     9301 ataaatttat tatcaggtaa gtcaaacaac actttaacag caccgaataa ttgctcaaag
     9361 ttatcaatag caacatcata aactggttta attcccggtt ttaaagaacg tcctctatgg
     9421 ttgaaccaca tagaatgcca accaccatta aatgcaccca taatatcatt atcatatgaa
     9481 tcacccacat aaagtgtagt tgaagggttc atatcaaatt gctctgctgc taaattaaaa
     9541 atctcctttt caggcttttg aaaaccagtt gcttgactaa ctataacacg ttttggatca
     9601 acatagtcat aaagtcccaa ttttttaact tttttcaatt gatgttccgt aggaccattt
     9661 gtaataattc ccattggtac attttttgat ttcaaaaagt caagtgtcat acgcatctca
     9721 tcaagcattg taatattttc aagttcatgt tcgtatattt cttgaaaata aataccagta
     9781 gcttcgttaa tttcacgata accaaactct aaaagcgttt ctttgcatcg ccagaaacga
     9841 aaatattctg ttgtccattc accagccatt actcgtggaa agccaatatc tgaataatga
     9901 cgaaagcgaa tataagcttg gttaatagcg ctcatatcaa aatcaggaaa gcatttctcc
     9961 acagcaatac ggtaaggtgc ttgttgatcg taaattgtat catcaacatc aaaaacaatt
    10021 gaagtaatca tgattatcct tttctttctg aattttcaat tgttaattat acctttttct
    10081 acccttattt tcaaatggtg aaaagtctat taaaaatgag aataacacca taaactcata
    10141 aaataaatta tgaattatca gtgtttttgt ggtacaatag gatgaatact aacttggagg
    10201 aaattatgtc taaccaacat attgaagaat taaatgacca acagattgtt cgtcgtgaaa
    10261 aaatggctgc tttgactgaa caaggcattg atccttttgg taaacgattt gaacgtacag
    10321 caacatctgg tcaattaaat gaaaaatacg ctgacaaatc aaaagaagac ttgcacgaca
    10381 ttgaagaaac tgctacaatc gctggtcgtt taatgactaa gcgcggtaag gggaaagttg
    10441 gctttgcgca tatccaagat cgcgaaggtc aaattcaaat ttatgtccgt aaagattctg
    10501 ttggcgaaga aaactacgaa atctttaaaa aagctgattt aggtgacttc cttggtgttg
    10561 aagggcaagt aatgcgtaca gatatgggag aactttctat caaggctact catattactc
    10621 acctttcaaa agcgctccgt ccacttcctg aaaaattcca cggcctaact gatattgaaa
    10681 caatctatcg taaacgtcat cttgatttaa tctcaaatcg tgacagcttt gaccgttttg
    10741 taacacgctc aaaaatcatt tcagaaattc gtcgctttat ggatagcaat ggtttcttag
    10801 aggttgaaac acctgttctt cataatgaag ctggtggtgc ttctgctcgc ccatttatca
    10861 cacaccacaa tgcacaagac attgacatgg ttcttcgtat cgcaactgaa ttgcatctaa
    10921 aacgtcttat tgtaggtggt atggagcgtg tttatgaaat cggacgtatt ttccgtaatg
    10981 aagggatgga tgcaacccac aatccagagt tcacatctat tgaagcctac caagcctatg
    11041 ctgactacca ggatattatg gacttgacag aaggtattat ccagcatgtt acaaaaactg
    11101 taaaaggtga tggaccaatt aattatcaag gcacagaaat taaaattaat gaaccattca
    11161 aacgtgttca tatggttgat gcggttaaag aaatcactgg tattgacttc tggaaagaaa
    11221 tgactcttga agaagcacaa gctttggcac aagaaaaaaa tgttcctctt gaaaaacatt
    11281 tcacaacagt tggtcacatc attaatgcct tcttcgaaga attcgttgaa gacactttaa
    11341 ttcaaccaac ttttgtcttt ggtcatccag tcgaagtgtc tccacttgct aagaaaaatg
    11401 atacagaccc tcgttttaca gaccgtttcg aactctttat catgactaaa gaatacgcta
    11461 atgcttttac agaattaaat gacccaattg atcagctgtc tcgctttgaa gcacaagcat
    11521 ctgctaaaga acttggcgat gatgaagcaa ctggagttga ttacgattat gttgaagcac
    11581 ttgaatatgg tatgccacct actggtggac taggtattgg tatcgaccgt ctctgtatgc
    11641 tgttaactga tacaacaact atccgagacg tcctactttt ccctacaatg aaataagaat
    11701 atattaaaac ccatccttat ttaaggtggg ttttaatatt acctctactg aactttgtta
    11761 ggtttctgat ttatcgctca cgcaattttt tatcaaggtt caccatttca atagccgaaa
    11821 gagcacaatc ataaccttta tttcctgctt ttgttcctgc cctctcaata gcttgttcga
    11881 tgttatccgt tgttagaaca ccaaagatat gagggatatc tgactggcta tttaaatgac
    11941 caataccttt tgtcacctca ttacaaacat aatcataatg atcagtgctg cctttaatga
    12001 ctaccccaag acagataata gcatcataat ctttatataa cgccattttt ctagccatat
    12061 aggggatttc aaaagcaccg ggtacccata caatatctat atcttcttct gaaacaccat
    12121 gtctcagcag tccatctact gctcctgata ataatttaga tgtaatcaat tcattgaaac
    12181 gtgagactac gattcctatt ttcatttcgt ttgccactaa ttgtccttca ataattgtca
    12241 ttttttcttc ctcctttaat ctaaaagatg tcccattttt tctttcttag tttttaaata
    12301 attaaggtca taagctgttg ctgctacctg taatggcact ctgtttttga cgcaaattcc
    12361 ttctgcttca agttgttgga ttttatctgg attattggtt agtaagttta tagaatttat
    12421 acctaaatct tttaaaagtt gagcactaac tccatagtct ctctcatctc cttcaaaacc
    12481 taaagccaaa ttagcttcta aagtatctaa gccttcttct tgcaaataat aagcttttaa
    12541 cttattaatc aaaccaattc ctcgaccttc ctgtctcata taaagaagga cgccacgacc
    12601 ttcagcctct atttgttgga gtgcattagc cagttgttcc ccacaatcac atctcaagga
    12661 atgaaagaca tcacctgtca aacattctga gtgtaaacga cataaaacat cttcaccact
    12721 actaatatcc cctttaacaa gggcaacatg ttccttactt gccacaactt cgctatatcc
    12781 ataagccgta aaatgaccga attgcgttgg tagctgaatc tcaatttctc taaccacccc
    12841 tccctcttct tgacgtctat agtcttgcaa ttgttttatg gtagcgatag ctaaatcgtg
    12901 cttttgagca aatgctaata actcatcctt tctcatcata ctaccatctt cagccattat
    12961 ttcacaacaa agtccacatt cctttaatcc agctagacga cataggtcaa cggttgcttc
    13021 agtatgtcca tttctagcta aaacacctcc ttccttggct aacaagggaa ataagtgccc
    13081 aggacgtcta aagtctttag ctttgctact gtcgttagca accatcttag ctgtcaaagc
    13141 tctatcttcc gcagaaatcc cagtactagt tgatagatga tcaatggata tggtaaaagc
    13201 tgtttcatga ttatcagtat tatggctagc catttgagct aattctaact tttcagcata
    13261 gtaattagat agaggtaagc aaattaatcc tttcccaaat ttagccataa aattaatcga
    13321 ctcttttgtc accatttcag ccgcacaaat aaggtcccct tcattctctc tattttcgtc
    13381 atccatcaaa actaccattt tccctgattt gatgtcttgt aaaagttttt ttattggact
    13441 aaaggccatt agctttctcc tctctcttag tagccatttt ctgataaaaa agaccaatcc
    13501 attaccgatg aagtgtgatt agttctatta gtcggtgcta aaaatttctc agcatatttg
    13561 cctaaaacat ccacttcgat atttaataaa tccccaacct tttttaagga taaattagtt
    13621 tgagtcatcg tgtggggaat gaccgaaact tcaaaagaat gctctgtaaa tgataccact
    13681 gttaagctaa tcccatctag agccaccgaa cctttctcta taatataaga cataatacta
    13741 ggtggagcat caattgaaaa tctgacagca atatcatctt tttggatttt ttctattttt
    13801 ccaaccccat ccacatgtcc aagaacaaaa tgtcccccta accttgagtc acttctgagt
    13861 gctaattcta agttaacttt agaatgaagt cgtaaagcag ctaaacttgt tcgcatcata
    13921 ctttctggca tgacgtctgc tataaaagct ttttcacttt tgtcaagaac cgtcaaacaa
    13981 acaccattaa cagctacgct atcccctttt cttaataaag ggactagttt gggagcgtca
    14041 atacttaatt gttgggactt tatgccattc cgaatacgac tgacttgacc catttcctca
    14101 ataattcctg taaacataat gttctacctt tccttcaata acaacatcag aatccatacg
    14161 actaagttca atatctttta ctctgaaagc ttggttaagc caatccaagc cttctccaga
    14221 aatagctgtt tttgctttca gacctcctat tattttgggt gcaataaaaa caattagtct
    14281 gttaacaata ccagctttta aagctgaaaa atgtaaacta cttcctcctt caataagaag
    14341 gctatcaatc ccgtcttgtc ctaaaatagt cattaatact ttcaaatcca gctttccatc
    14401 ttttcttgga accttaatca aacggcaacc catttctttt aatgtttgtt gctgagcaag
    14461 attatctgaa catgttgcaa tccatgttgg tattgtttta gcactcttaa ctaggtaaga
    14521 gtctaaaggt atcttcaact gactgtcaca tacgatcctc acaagcgctt cacctttagg
    14581 aatacggcaa gttaaacgtg gattatcagc taatacagta ttgataccga ccataatagc
    14641 actacatttt tgcctcaatt tctgaacaga ttgtcttgag tgctcatttg atatccactt
    14701 agaatcgccc gtctttgtag ctatcttccc atctaaagtc atagcatatt tcaaataaac
    14761 aaaaggctgc ttataggtca tgtgaaaaat gaatctttca ttcagagcat cgcattcttc
    14821 tcttaagata ccaacttcaa catttaaacc ttcttttctt aacaaggcaa tcccttttcc
    14881 tgaaactaaa ggatttggat ctaaacttcc aactactact tttttgatac cagatttaat
    14941 aagagcttca gtacaaggtg gttgcttacc cacatgacag catggttcta aagtcacgta
    15001 cagagtactt ccagagatat cttcatcagc atttttaata gcctgtcttt ccgcatgtaa
    15061 atctccaaat cgtttatgat aacctttgga aattatacga tcatctttaa caataacagc
    15121 acctactaat gggttgggag caacgaaccc catacctttc tcagcctctt tcaaggctaa
    15181 tgccatgtag tcttcttttt tcatcatttc ctttttcctt ctaaaataaa aacccttagc
    15241 ttctaaaata gaaaactaag gggagggatg agaagtcgtt gattatacta attagcatta
    15301 tattttaggt agcacaaaaa aactaaagac tccctataga aaactatagg gagtctctca
    15361 ttaatccagc acaaataaac tgcaagtagc agacaatcac tgaattcttc tctcatccag
    15421 actttactgt cggtactgga atcacaccag ttcatgctcg aaagctcgcg gactataccg
    15481 ccggttagga ttttcacctg accccgaaga aattctattt gacagttatt ttactaaact
    15541 catttttatt tgtcaataaa aataatgtgt tttttattat aaaataataa ttaagtagct
    15601 catctattct tatctttaaa agacagtata tctataccat aaagcaatct ttcgaactaa
    15661 tcaaacaaga atcgatagta ttattggatt atatttctta aaaaattcac tacagatata
    15721 gtttactcca cttaaataac ttaggtaaat ctacggaact aaaaataatc gatagaaatc
    15781 tgttattttg ttatttcaaa tgtaactgtt aagaaaatag ttctctaata ctaaacagca
    15841 cctcaatcga ttattaagta gcgttaattt ttaattgttt ttctattttc tatttgttat
    15901 aatggcttga gattactaga aagtatagta ttacatgaat caaaatcatt ccgtcaaaag
    15961 atccttatcc gaagttaatc agagcgttga ggttcctcac aatagctctt tttggaacac
    16021 tttaagagct tttcttggac ctggagcttt ggttgcagtt ggttatatgg atccaggtaa
    16081 ctggattact agcgttattg gtggagctac ctatcgttac ttactattgt ttgttgtttt
    16141 agtatcttct ttgatggcga tgcaattaca acaaatggct ggcaagcttg gcattgttac
    16201 acgacaggac ttagcacaag caacagcttc tcgactccca aaaccgcttc gttacctgct
    16261 atttattatc attgagttag cccttatcgc tactgattta gcagaagtca ttggctctgc
    16321 cattgctctt cacttattat ttggctggcc cctgttactg tctatcatga ttactatttt
    16381 ggatgtattc ttacttttac tcttaatgaa gttaggcgtc caaaaaattg aagcctttgt
    16441 ttctgttctc atattgacaa ttttgataat ctttacatat ttagttgttt tatcacaacc
    16501 tgatttagat gctatgttca aaggattttt accacaccat gagcttttta acatatcaca
    16561 tgaagggaaa aatagtcctt taaccctcgc tcttggcatt atcggcgcaa cagtcatgcc
    16621 acataatctt tatttacatt cttctttatc tcagactaga cgcgtggatt atcataataa
    16681 atcttcaata aaaaaagctg tacgtttcat gaccttagat tctaacattc agctatcact
    16741 tgcatttgtc gtgaattcgc tactattagt cttaggagct agtctatttt atgggcatgc
    16801 taatgatatc agtgcctttt ctcaaatgta tcttgcccta tctgataaaa ctattactgg
    16861 tgcagtagcc agttctttcc tttctaccct ttttgcagtt gccctactag caagtggtca
    16921 aaattcaacg attacaggta ctttaacagg acaaatcgtt atggaaggtt tccttcactt
    16981 caaattacct caatggttaa taaggctttg tactagatta ttaactcttc ttccaatttt
    17041 tgttatcgcc ctactagttg gaggcgaaga aaatacgtta gatcaactaa tcgtctattc
    17101 acaagttttt ctttccttag ctttaccatt ttcaatcttt ccattgattt atttcacgtc
    17161 gcaaaaatca attatgggag agcatgctaa tgctaaatgg aatacctatt tagcatacct
    17221 tgtcgccatc attttaacat tactcaattt gaaattaatc atggatttac tttaatacaa
    17281 tacaaaaagc cccactaatg aactgaacct ctatcatgag acatacatct aatgatagag
    17341 gaaagtacat aatgatggag ctttttgtat ttcttttaaa gaacccgtga gtaaggacta
    17401 gtattttttt gcttgtacct ctccttgaca ttataataac cgatgatgaa aaacgatacg
    17461 ccaaagataa ttgctgcaat caaagataaa atttggatac taaaatatag gtgttgaaaa
    17521 tttccttcta aatgtgcttt aaaaaccatc aaccaagccc aaaaaacgga taagattaag
    17581 taagcatttt gtaataccct agccaggtaa agacttaaaa cagttataaa aatcaaggca
    17641 atgatagcta tatatacctt tggtaatggg taattaaaat caattttaac taagtagacc
    17701 gctaaattga ccaatgacgc aactagcaac catcctgcat gaattccaaa tgttattcta
    17761 agcagtaatt ttgatttatt tttactgagt atcttgataa tgataaccag aagaatacag
    17821 taagcaaaaa ttattattgt tgataggcca atcaaattac tcgtaaaaaa tacagtccat
    17881 atattattaa ctaatagaag acccaaaaag tatggaaaaa tgctagcaaa gttatcttgg
    17941 taagattgac tcttactgaa tatagctctt aatattacta aaaaggtcaa taaatagatg
    18001 actgaccaaa ttgaaaaggc taatggtgct ggtgtcaaga gcgtttgata acgatcagac
    18061 agactcttct gactgttccc tgttaacaaa cctgttgcgg ataaataatt cactattatt
    18121 gttaagataa agactaacaa taacatctta cttttaaatg aatatctaat catagaggcc
    18181 tcctttcacc tccattataa ttcttttagt ttaaaaattc tattttttta atcaatatgt
    18241 ttactgaata ctcgcttgtc tcgacaaaaa aagtggtttt tgaaaccact ttttatttat
    18301 ttacgaaaag catctaaaat gactttaaat tcttcattag ataaagtgat acctttaccc
    18361 atttttgtat gatctggact ccatgtacgc aaatcaaatt tagctggcgc tccgttgaag
    18421 ctaacacgat tcaactcttt tgtccaacct ttttcatttt cagataggac cagtaacttc
    18481 tcaacaattt caaatgtaaa ttctgacatt ttatacctcc acttatacta ttcgttattt
    18541 ttatagcttt ttgttctatt taaatgaact tcttctattt taacaatttt tagtaatatt
    18601 gaccatgtaa aatgacaaaa tagttataaa agtatctaag ctcttatagt cttgctagta
    18661 ccatcatttt gataaaggtt cactaatcct tctttacaag cacgaatcat atcccctttt
    18721 tttactggat ttggtaatgt tatggttaat aattccatcg gatttggagc acggtctatt
    18781 ttatttccct cagcatcgcg cagaccttca ataaaacatt caaagtgacg gaagccagga
    18841 ccatagaatt ctacgcgatc tccttccata ataacattac gctgacgaat tgtagcctcc
    18901 attttagcat tgtcaaatga aaccacttcc ccaacaaatt tgtattgagg aattttacga
    18961 cgagctccaa agagttgttc attttcagtt ggtgtatggt agtaaaaacc tgttgctaat
    19021 tcgcgttgtg caaccttcca aagttcatca atcaagtctt ctttaatagc ttcaaaagct
    19081 tctggacttt ccatataggc atctacagca gctttatagc aattagttac tgtagaaaca
    19141 taatgaatgg atttcatacg tccttctatt tttaaactat ctacgccatt ttcaatcata
    19201 tctggaatat gctcaatcat acacatatcc acagctgaca ttgagaaagg ttctggaatc
    19261 tcgcctttta acgattgacg ttcttgtcca aatggcatat cgtagaggtc atatttccaa
    19321 cgacatgact gagagcaacc gccacgatta gcatcacgat ggctcatatg gtttgataga
    19381 acacatcgtc ctgagtatga aatacacatc gcgccatgaa caaatgcttc tatctcaaca
    19441 tctgtcctct tcctgatttc agccaactct gccatagtaa cctcacgtgc taatacgaca
    19501 cgagtaagcc ccatctcttt ccaaaattca aaagtttcat agttcgtgga agaggcttga
    19561 gttgacaaat gaatttctaa acctggtgct tctgtagcac aaataacaat aagagcagga
    19621 tctgaaacaa tgactgcatc tagtcccata tcacgtaatt cacgaaacca cggacctgcc
    19681 ccaagctcat taccttcatg agtaaccata ttagccgcta cataaacttt agcatctctt
    19741 gcatgagcat agtttattcc ctcttgtaac tcttccatag aaaagttacc agctctactt
    19801 cgaagaccat acgcttgacc tccaacaaat acagcatctg ctccataatc aatagcaact
    19861 tttaattttt ctagtgttcc tgcaggtgat aaaacctcag ggcgtttttt tacattagac
    19921 atcttatatt tccttctatt ttcaagataa aagaattatt ttctatctta attaacatac
    19981 caatcgatat gcagttctta gtgtaattgg ctacgctttg aaatagaaaa ctttcacgta
    20041 gcaaataaat cttattttac tgttgatggg tcaaactcat aaaatccagt atctaaacca
    20101 cgtcctttag ggtgcagttt tctaattcgt tcatcaaaca agaaagcctg atcctgagtg
    20161 aatgttccat tttcaatcaa acgagctgtt tcgacaaaaa tttcagcaat agcaacaaaa
    20221 ttatctcctg gacaataaat accatctaat ttccagtgag taaagtgatg ttcaactagt
    20281 tcagtcactt tagtcatcat atcgatatca ttgttaataa aaatatgagt cccatgctta
    20341 tcttcgtaaa tagaatagtg actttctgga tcacctggtt ctgctaaaaa aagaccacgc
    20401 tcacgtgttt tttcatcagt gatatgagta aaattataat agttttgtaa taacggacgc
    20461 ttagagtgat gaattacaga agccccataa actaaaatct cagcaggaaa ttcaaggttc
    20521 tcagacatct taaaaagttc ttcagaaggt atttcacgtg ctaaaaccgt ttctactgct
    20581 ccatgttgtc cccaaaaatt aacttgacga cttgatgtaa cgaatacaga agtgtcgtag
    20641 ataagtttga agttataacc atcacgcttg ttgatataaa atactcctgc atcaccaact
    20701 accaaataat ccacattaat ttctttcatc aactccaaaa atggtttgat attatccatc
    20761 atctcttgat gcatgagtgc attacaagct actgtcaatt ctttaccagc atcatgtact
    20821 aatttagcga tttcacgcaa ttcatcgtct gaaaatgaat gtggtaaacg aagaccgtaa
    20881 ttttcttccc ctacgtagat gcggtcaatt cctattgcta gcaattgctt tacttgttca
    20941 atagattcag ctgtcgctgt caaaattatt ttttccataa ttagtattat aacaaacttt
    21001 cagcattttt aagcatattt cttgacaaga ataaataaaa aatattcttt tcgaaaatta
    21061 tatcacttta gaggttaagc actatcagat aagaaaaaac atagtattga aatactatgc
    21121 gaaattatat ctattttttt cctttccaat ttaaaaaagt aaggaaagta gtaaaaatac
    21181 ttttagtatt ctactactaa tatggacaat gtttttgttt ttttgagaaa aaacctttat
    21241 tttaactcat agtatcaaaa aactagtgac aaactctatt atttgtgata tgatatgcta
    21301 gtatcaaagg agaaagctat gtcatcacca cttaagcaag agcaaactca cgaaagcaaa
    21361 acagagcaag ctcaaccaaa atttcaagta tttcaagaat ttgatcacag tgctaagttg
    21421 cgtgagctag tcttttttgc tcgtatcgct ctcttcagta taaccactgt gctaatctct
    21481 ttcttattac tagcactaag tttaacacct gtctggagtt tcttactagc gatgctggca
    21541 agtctagcag ttacatctgc agtctcttca ttcatttggt cactcagaca ataaaaatct
    21601 cataaaaaag aagctactat aagtagcttc ttttttattt ttctttatct tctgtgtttt
    21661 ctttataatc aatgacaata tcttctactt ttgtttcagg agccttattt tcttttttag
    21721 cagtatcctc atctgagaat tttgatttag cttgattgac aaaatcatta gcaaagtcaa
    21781 ctacttctcc gcttttttcc ttaacggctg agacgatatc ctctgttgtc aattcacctg
    21841 attcaaattt acctttataa tctttaaaag tatcaacagc taaatttgaa tattcacttg
    21901 ctttatcttt agctatttga tgatattctt ctggattttc tttatattct ccatagaact
    21961 tttctgcatt tttccttagc tcttttcctt tttttgttgt taaaaaataa gctgctgctg
    22021 cacctgatgc agcactgata actaaagttt taaataattt actcattttt tgtctccttt
    22081 tttacgaaaa atttttgaag ccgcttttcc aatagctaaa gctgaccctg ccttagtaac
    22141 acttgaggtt gcactacttg ccttttgtcc aatgtgtctt gcctgtaggt ttaaatcaga
    22201 tacagattct gataagtcag caatagctac aaaaagagga tcaatagtag atactttacc
    22261 attaacatca tcaactaaaa cattagcttt ggctaaaatt tcattagttt ggtaaagagt
    22321 cacgttaaca tcgcttgtta atactttaat agtttgcttg gtttcctcaa tcgtttctga
    22381 tacttttttc agaacgaaaa gtattcctaa aactaaaaca acaaacgcaa tggcaataat
    22441 taatacagca atttctatca taatacctcc tctgtttaat aatatgaaat atgttgttca
    22501 cgacgacgat aaataatcat gacaattccg ataataacta aaataactga taaccattgt
    22561 gacacgcgca aacccgccaa ataaaggcta tctgtcctca tcccttcaat aatgaaccgt
    22621 ccacatccat accaaactaa ataaaaacat gccacttcac cttgttttaa agtgcgcggt
    22681 cgatgacgaa tagacatgat gatgacaaaa cctaggaggt tccaaagaga ctcatataaa
    22741 aatgtaggta ctcggtaagc tccatcaata tacatttgat tttttataaa gttgggtaag
    22801 taattcagat tttttactgc cctaccgtat gcttcttgat taataaaatt cccccatcta
    22861 cctatggctt gggcaatcat cactcctgga gcagcaatat ccagaaaatc aatgggatta
    22921 atcaagcgac gatatgaaaa aatcacaagg agaatggctc cggtaattaa ccctccgtaa
    22981 atagcaatac caccgttcca aatggcgata atctctactg ggtgcttaga gtaataagcc
    23041 cattcgaaga taacataata aatacgagct ccaacaatgg atagtggaaa agccatgaga
    23101 atgaaatcta gaatatcatc cgacttaatg ttcttacgag gggcctcttt cattgctaag
    23161 taaactgcta acaacatacc agacactatg caaatagcat accagcgaat agaaaagggg
    23221 cctaattgta ttgctactgg attaatcatc attgacctcg ttctgactaa tcaaatgtgt
    23281 taaacgatcc tcaaaagtct gggtagcatc aaaccccatt tgtttagctc tgtgattcat
    23341 agccgctgct tcaataacta ctgacacgtt tcggcctgtt ttaacaggaa tacgtatccg
    23401 aggaacctta actccagata attcaatctc ttcattgcca tttccgagtc tatcaaaaac
    23461 ttttccagtt tcaaagtttt caaggtatat agctaattgc acttgcgatg aatctttaac
    23521 cgcactagct ccatataggc tcataatatc aatgattccc acacctctaa tttcaagtaa
    23581 atgacgtaat atctcagcag gctctcccca aagcgtctct tcgtctttag cataaacatc
    23641 aacacgatca tctgctacca aacgatgtcc tcgcttaact agctctagtc ctgtctcact
    23701 cttaccaata ccggaatctc cttgaatcag aacacccata ccatagatat ccatcaagac
    23761 accatgaaca cttgtcctct cagctaagca agaatctaaa taccaagaaa cttcacctga
    23821 taaacgactt gtaggggcct tactttgaag aatagcaata ccggtatctt tagccgcctc
    23881 atacatctct tctggaattt ctaaatcacg tgctacaaca atagctggtg tttctttttg
    23941 gaacatctca cgtaacactt gataacgatt atgtcctgtc atagccatta aatatgacca
    24001 ctctttcata ccaacaagtt gcaaacgctc tggtgcataa taatcaaaat accctgtcat
    24061 ttctagacca ggacgtgaaa tgtcagctgt tgtaatccgc ttagataata gatgttcatc
    24121 accatagatg acatttaact ttaagcggtc aactaacatt tgaacagtaa ctgccatact
    24181 tactccttct tataaaagtg aatatcccta ttgctatacc ctacaattta aagttgagca
    24241 aaggtactaa acactttcat taatttacac ccacattata tcatactttt tactaaaagt
    24301 aagcgctaac gcgtttttct gaaaaaaaga agatattttg aaaaaatggt ctaaataaag
    24361 ctaagaaaaa aggagattcg tctagcaaat ctcctttata tgaattacca gaaccaaccg
    24421 tcatcatcaa caggttcagc atctttacgt cttctgggac cttgtcttct tgtttcaata
    24481 atatcttctt tatagggtaa gaaaacagct aataaaatat atagcaaaag accaaatttg
    24541 gtaaaataca aaatcagtgc aatcaacacc cgtgaaagag ctaaatccca gttatactta
    24601 tcagctagac cggcgacaac accgcagacc aacttacctt ttcgttgttt gtagaagctt
    24661 gatttcatac ctatccttct ttctatctac tctatatttt aattataact actttgacag
    24721 taaaattaaa taggccttac gactgatttt aaaaactctc tatcaagcga ccatgacaat
    24781 aaccacaagc aaacttgcgt aaattaacac gtctcctacg tcggtatact tgaccacaag
    24841 attggcaaga gtaatgatga taattcactt taacagccgt aactctaggg gtatatcgta
    24901 agccatcgac tttttctagt aactctttaa aagctttatc cctatgttga tatcctttac
    24961 cttgtatata gagatgataa tggcaaagct catgtcttac aatcttacga aaaatatcaa
    25021 taccaaactc acgataaatt ttgggattaa agtcaaggtg tctatcgttt ggaaagaagc
    25081 gtcctccagt tgttctcaag cgattattcc aatgtgcagt atgtaaaaac tgacatccaa
    25141 aatcttctat agacaccttc ttaacataat tagtcaagtc cgtctggctt gataagacta
    25201 agtccaattc tgctacgctc cgcatctacc tctacaaccc aaacagttac aatatcacca
    25261 acagagagat atttgcttgg atgtggtaaa ggaggcattt ttctagtttt tttatcgcgt
    25321 tttcgcttaa taaggcgaga ctggtggatt aaaccatctt catgaacacc aatatcaaca
    25381 aaagcaccaa aatcaacgac gtttcgaaca gttccttgca attcctgtcc aacttttaaa
    25441 tcactgacat ctaaaacatc atgtctcaaa acaggcgctt caaaatcatc acgtaaatca
    25501 cgacctggtt tcaaaagatc ttctataatg tctttcaatg tttcttgacc taccccaata
    25561 ctttctgctg ttgcaattaa gtcaaggttt tgtaacttct ctttagcaaa atcatctaat
    25621 tctttgatag tcagttggtc aagcaacttt ttaacagctt cataagactc agggtgaaca
    25681 cctgtattat ccaagaaatt tttagcatta ggaatacgta agaatccagc agcttgctca
    25741 aaagcttttg ctcctaatct gggtactttc ttaatttcag cacgtgactt aatttgccca
    25801 ttctcttcac gatatttaac aatattttca gatattgttt tatttaaacc tgaaacgtga
    25861 gctaacaagg ctggacttgc tgtattaaca ttaacaccta cttggttaac tacagtttca
    25921 acaacaaaat caagattttc tgctaatttt ttttgactaa catcatgttg atattgaccc
    25981 acaccaatag attttggatc aattttgact agttctgcta atgggtcttg cagacgtctt
    26041 gcaattgaga tggccgaacg tttttcaacc gttaagtctg gaaattcatg acgtgccaat
    26101 tctgaggctg aataaacaga ggcaccactt tcgttaacaa tcacatagga tacgtctgga
    26161 aaatcttgta aaacttctgc aacaaatgct tccgattcac gactcgctgt tccattccct
    26221 atagcaataa tttctatatt gaattctttg ataagttttg ctaactctat ttttgattgt
    26281 tcgatttttg cttgattagc tggaggaact ggataaatca cttgtgttgt cattaatttt
    26341 ccagtttggt ctactactgc taattttgct cctgtacgaa aggcagggtc aaaacctaac
    26401 accattttcc ccttaagcgg tgagactaac aataagttac gaagattctc tgaaaacagt
    26461 gaaattgcac catcttctgc tccttcactt agttctgtac ggatacggcg ttccatagct
    26521 ggaacgattt ttttcttgac agtctgtaca ataaggtcat ctatatattg gctagtttct
    26581 ttaaatcgaa cagcaaaaaa gcgaaacatt ttttcaaggt tatgctcaaa attaacctta
    26641 agaacaccca ttttctcacc acgattcaat gctaaaactt gatagccatg gagtttactt
    26701 attttttcag aaaagtcata gtagattttg aagacctgtt tttcatcaag tgactcatct
    26761 ttaacaactg cagtgattga gctgtagttc caaatctcat tatatgtcca tgaacgtaat
    26821 ttattatcct cagaaaaagc ttcgataaga atatctactg ctccagaaag tgccttcgta
    26881 gttgtctcaa aaccatctgt aagatagttt tgagcttctt cctcaaggtt atccttattt
    26941 tgtaaaataa gacgtgctaa aggaaaaaga cctgcttcac gtgcaatcgt agctttcgta
    27001 cgacgttttt ctttataagg taggtagagt tcctcaacat ctgctagttt agttgcttct
    27061 tcaatagcct ttttgagttc ttgagtcaac tttccttgtt cttcaatttt ggctaagacc
    27121 gtagttttac gatctgaaag agctgtcata ctcttatcaa ggtcaataat tgatttgatt
    27181 tgaacttcat ccaaattacc tgtcatttct ttacgataac gggcaataaa aggaatagta
    27241 tttccttcat ctgttaattc taaaaccttt tcgatttgtg acgctttaat acctaaatca
    27301 atagcaattt gtgtaatatt taaattttcc ataacgcttt tattatagca tatttttgac
    27361 ttacgaagag ttttaaaaag cgatttcaag atagcgtttt tataatctaa caataaaaaa
    27421 cagaccaggt ctgctttaat tcttttcaaa atattttcga acaattggtg ctacttcctc
    27481 accataaagt ttgatggcat ttaggacgtc tttgtgaggc atagagccaa ctggtagatg
    27541 taacataaaa cggtctaact caagcgcttc aacaagtcct ataattttat gtgcaaccac
    27601 ttcaggactt cctacaaaaa tagcaccttc tggacctact gaacgtaaat actgctcttt
    27661 agtcatttca gaccaatgag ggcgtccctt agcaatatta tcgacggtct gtttcgtagg
    27721 gaaaaaataa cggtcaatag cggtttgatt atcctcttca atccatcccc aagagtgagc
    27781 ggcaactttt agttgttctg gtttgtgtcc attacgacta ccaacttctt tataaatatg
    27841 gaccaattga cgaaaggctt tgggattccc accaatagtt gcataaacaa ttggcaaacc
    27901 ttgttctgca atacgaattg tagaatcaac atttcctcct gttgccaccc atattggtaa
    27961 ctgtctttgt aatgctcttg gataaattgg tcgctcatta actgtttgtg tcaaatgacc
    28021 tttccaatcg agatttgtcg ctgagttaat tgctaacaac atatccattt tttcattaaa
    28081 taaatcatca taatccgcta aatcgtatcc aaacaatgga aaagactcaa taaaggaacc
    28141 acgccctgcc ataatttctg ctctaccatt tgaaagtgcg tcaatcgttg aaaattgctg
    28201 gtagacgcga ataggatcat tggaagagag aatcgttact gcactagata aacggatatt
    28261 attagttcta accgctcctg ctgctaggac aatttcgggt gcagagaccg caaaatcttc
    28321 acgatggtgc tctccaatac catagacatc taaaccaact tgatcagcta gttcaatctc
    28381 agcaactaat tggcgaatcc tctcaggatg tgaatagctt tggtttgttt cttcaaggat
    28441 tgttgtctct ccgaatgttg taatacctaa ttcaatcata atcttacctc atcaatctac
    28501 taataatatt ttatcactat atagtattat ttccaatcga tttgtttttt tatgttaaaa
    28561 ataagctaat atagtataat aggttattat ataaagaaag aggtgccatt aatggctatt
//
LOCUS       DATRTN010000016        28253 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.016, whole genome shotgun sequence.
ACCESSION   DATRTN010000016 DATRTN010000000
VERSION     DATRTN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28253
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.016"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(57..2885)
                     /gene="uvrA"
                     /locus_tag="VBE22_000762"
     CDS             complement(57..2885)
                     /gene="uvrA"
                     /locus_tag="VBE22_000762"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEO7202234.1"
                     /translation="MQDKLMIRGARAHNLKNISVDIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSVEQIVDKVLALPERTKMQILAPIIRRKK
                     GQHKSTFEKIQKDGYVRVRIDGDIHDVTEVPELSKSKMHNIDIVVDRLINKEGIRSRL
                     FDSVEAALRLSDGYVVIDTMDGNELLFSEHYSCPECGFTVPELEPRLFSFNAPFGSCT
                     TCDGLGIKLEVDIDLVIPDKSKTLREGALVPWNPISSNYYPTMLEQAMTQFGVDMDTP
                     FEKLSKAEQDLALYGSGEREFHFHYINDFGGERNIDLPFEGVVNNINRRYHETNSDYT
                     RNVMREYMNELKCNTCHGYRLNDQALCVRVGGEEGLNIGQVSDLSIADHLELLETLRL
                     SSNEQLIARPIIKEIHDRLSFLNNVGLNYLNLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYVLDEPSIGLHQRDNDRLIDSLKKMRDLGNTLIVVEHDEDTMMAADWLIDVGPGA
                     GAFGGEIVASGTPKQVAKNTKSITGQYLSGKKVIPVPSERRVGNGRFLEIKGAAENNL
                     QNLDVKFPLGKFIAVTGVSGSGKSTLINSILKKAVAQKLNRNSDKPGKYVSLEGIEYV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     ESCSGDGIIKIEMHFLPDVYVPCEVCHGTRYNSETLEVHYKEKNIAQILDMTVNDAVT
                     FFAAIPKIARKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHADDIARLLKVLDRFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGIGGGQ
                     IVAIGTPEEVAENPKSYTGYYLKEKLAR"
     gene            complement(3017..3688)
                     /locus_tag="VBE22_000763"
     CDS             complement(3017..3688)
                     /locus_tag="VBE22_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HEO7202235.1"
                     /translation="MTLQDLTKKNQEFVHIATNQLLADGKSDAEIKAILEEHLPEIID
                     NQKKGITARSLLGAPTTWAASFTERPEDKARVSVQKNTNPWLMWLDTSLLFLGLVTAL
                     NGLMLLFGQSNVNTGLISILTLGFGGGAAMYVTYYYIYRHMGKPKSERPGWLKSFAVL
                     ALVMLVWFALFAVVPLLPATINPKLPEVVLFIIALASFGLRFYLQRKYNIQSSMAPVA
                     PQQRR"
     gene            complement(3713..4657)
                     /locus_tag="VBE22_000764"
     CDS             complement(3713..4657)
                     /locus_tag="VBE22_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO7202236.1"
                     /translation="MKQMFLSTAIEFKEIETFEPGAWINLVNPSQEESEQVADQFGID
                     IDDLRAPLDVEETSRISVEDDYTLVIVDVPTYEERNNKSYYMTIPMGIIVTDNAVITT
                     CLEHLTLFDHFYRRRVKNFYTFMKTRFVFQLLYRNAELYLQALRTIDRQSDKIEAQLE
                     SATRNEQLIDMMELEKSIVYLKASLKFNERIVKKLTSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMANIYENVLNAMTETTASIIGNNQNTIMKTLALVTMTLDIPTVIFSAYGMNF
                     QNNWMPLNGLAHGFIYVVLLAFLMSSFVVFYFIRKKWF"
     gene            complement(4827..5066)
                     /locus_tag="VBE22_000765"
     CDS             complement(4827..5066)
                     /locus_tag="VBE22_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEO7202237.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(5111..5602)
                     /locus_tag="VBE22_000766"
     CDS             complement(5111..5602)
                     /locus_tag="VBE22_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO7202238.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFSLAVNRNFKNQSGER
                     EADFINCVIWRQQAENLANWAKKGALVGITGRIQTRNYENQQGQRVYVTEVVAESFQL
                     LESRATREGGSPNSYNNGGYNNAPSNNSYSASSQQTPNFSRDESPFGNSNPMDISDDD
                     LPF"
     gene            complement(5614..5901)
                     /locus_tag="VBE22_000767"
     CDS             complement(5614..5901)
                     /locus_tag="VBE22_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEO7202239.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILSDNGATVVESKDWE
                     KRRLAYEIQDFTEGLYHIVNVEAEDAVALNEFDRLSKINGDILRHMIVKVD"
     gene            6994..8118
                     /gene="mutY"
                     /locus_tag="VBE22_000768"
     CDS             6994..8118
                     /gene="mutY"
                     /locus_tag="VBE22_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEO7202240.1"
                     /translation="MWPEDRIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YSKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFQREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWAAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            8295..8888
                     /locus_tag="VBE22_000769"
     CDS             8295..8888
                     /locus_tag="VBE22_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7202241.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSRQSISNWENDKTYPDI
                     YSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFERDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQTYKEILAVSSGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITVATFFASRFIFTWLFHLS"
     gene            complement(8934..9248)
                     /gene="trxA"
                     /locus_tag="VBE22_000770"
     CDS             complement(8934..9248)
                     /gene="trxA"
                     /locus_tag="VBE22_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEO7202242.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(9329..9829)
                     /locus_tag="VBE22_000771"
     CDS             complement(9329..9829)
                     /locus_tag="VBE22_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO7202243.1"
                     /translation="MESYEQFYAKLSQPFRKSPQLIILLNFLLKIVTGMMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRLDFPRPYEKWNIKPLIYKDTEGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLIL
                     GLCLFI"
     gene            complement(9833..12172)
                     /locus_tag="VBE22_000772"
     CDS             complement(9833..12172)
                     /locus_tag="VBE22_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEO7202244.1"
                     /translation="MNNKILEQLEFNKVKELILPYLKTEQSQEELSELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLVIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEIEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDNFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(12257..12799)
                     /locus_tag="VBE22_000773"
     CDS             complement(12257..12799)
                     /locus_tag="VBE22_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEO7202245.1"
                     /translation="MLSLLLLIIVIWHFYIGYSRGIFLQVFYVLMSMVSLMIASQFYQ
                     ELASQITLWVPYSNPVQGVEVYFFKDISKFQLSHVYYAGVAFVFIYSLSYLVGRLLGV
                     LLHLAPVEHFDSLQNNIISGFLAVLVCLLFMSMCLTILATVPMSFVQEKLWNSLFVRF
                     LINDLPFFSQFLVRTWAVGL"
     gene            complement(12802..13113)
                     /locus_tag="VBE22_000774"
     CDS             complement(12802..13113)
                     /locus_tag="VBE22_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202246.1"
                     /translation="METKINNSQKNRYKFVFGDKPLTLTTDKDNLFMEEIERVATEKY
                     EAIKEKLPNADNETIAILMAINALSVQLSREIDIEKMEDELNKLRSKTISDIKEKVSE
                     D"
     gene            13224..14117
                     /locus_tag="VBE22_000775"
     CDS             13224..14117
                     /locus_tag="VBE22_000775"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEO7202247.1"
                     /translation="MNTIVMQADKKLQEKIRTDLAQHHISNNNPYVVFSAKISGTTVL
                     LYTSGKLVFQGSNASHIAQKYGYIEQKESCSSETQDIPIIGTDEVGNGSYFGGLAVVA
                     SFVTPKDHAYLKKLGVGDSKTLTDQKIKQIAPLLEKAIPHKALLLSPQKYNQVVGPNN
                     KHNAVSVKVALHNQAIFLLLQDGFEPEKIVIDAFTSSKNYQNYLKNEKNQFKQTITLE
                     EKAENKYLAVAVSSIIARNLFLENLNKLSDDVGYKLPSGAGHQSDKVASQLLKAYGIS
                     SLEHCAKLHFANTKKAQALLK"
     gene            14133..14726
                     /gene="lepB"
                     /locus_tag="VBE22_000776"
     CDS             14133..14726
                     /gene="lepB"
                     /locus_tag="VBE22_000776"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO7202248.1"
                     /translation="MKEFIKEWGVFILILSLFLLSRIFLWQFVKVDGHSMDPTLADKE
                     QLVVLKQTKINRFDIVVANEEEGGQKKKIVKRVIGMPGDVIKYKNDTLTINNKKTEEP
                     YLKEYTKLFKKDKLQEKYSYNPLFQDLAQSSTAFTTDSNGSSEFTTVVPKGHYYLVGD
                     DRIVSKDSRAVGPFKKSTIVGEVKFRFWPIRRFGTIN"
     gene            14855..17275
                     /locus_tag="VBE22_000777"
     CDS             14855..17275
                     /locus_tag="VBE22_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEO7202249.1"
                     /translation="MEVFFTGTIERIIFENASNFFKILLLEIEDTDSDFDDVEVIITG
                     TMADVIEGEEYTFWGTLTQHPKYGEQLQSVRYERAKPTSGGLVKYFSSEQFKGIGKKT
                     AQRIVELYGDNTIDKILESPEQLSTISGLSKINREAFIAKLKLNYGTEQVLAKLAEYG
                     LSNRAAIQIFDHYKEESLEVINENPYQLVEDIQGIGFKIADQLAEQVGIESDSPKRFR
                     AAIIHTLVESSMEQGDTYIEARTLLEKTITLLEEVRQIELDPSIVAKELTNLIAEDKV
                     QHIGTKIFSNTLFFAEEGIKKNLQRILNQPLDKQLSHKDIDREIRDIQKSLNIHYDNI
                     QEKAIREALLSKVFILTGGPGTGKTTVINGIIEAYSELHHIDLNKNDIPIVLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDSDYQSLDDYLDCSLIIIDEFSMVDTWLANQLFD
                     ALDSHTQVIIVGDSDQLPSVGPGQVLADLLNINALPHIKLEKIFRQSEESTIVTLANQ
                     MRQGFLPEDFTAKKADRSYFEASANIIPNMISKIVQSALKSGIEAHEIQILAPMYRGQ
                     AGINNLNLIMQNLLNPLKDNNQFTFNDINFRIGDKVLHLVNDTELNVFNGDIGYITDL
                     IPAKYTESKQDEIYMTFDGQEVIYQRKEWLKITLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRMLQRNLIYTAITRSKSKLILLGEIGAFDFAVKNEGAKRNTYLIERFENKQEIANS
                     QKIEDSSIDQKIDNTIINTSIPKTATPIEQTNLSKITYRLTEENYLTIDPMIGINQQD
                     ISAIFDSK"
     gene            17389..17871
                     /locus_tag="VBE22_000778"
     CDS             17389..17871
                     /locus_tag="VBE22_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202250.1"
                     /translation="MVSYEKVRRSLRTATITIIVLNSLSLVFRLFTGISVQLAKTEIN
                     KGNTGNLPKEHIEAVLSATTPFMLFVTALIVLVNIAIVIFCIKNLRAIKRNQMVNYLP
                     YYLGFAITVGLVILGFLTTKAPWAIAINIVFQAIFGLLYFHAYQKAQKLNERDLEETN
                     "
     gene            complement(17942..19051)
                     /gene="dinB"
                     /locus_tag="VBE22_000779"
     CDS             complement(17942..19051)
                     /gene="dinB"
                     /locus_tag="VBE22_000779"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEO7202251.1"
                     /translation="MGERTMLIFPLINDTSRKIIHIDMDAFFASVEERDNPSLKGKPV
                     IIGSDPRKTGGRGVVSTCNYEARKFGVHSAMSSKEAYERCPQAIFISGNYQKYRQVGM
                     EVRDIFKKYTDLVEPMSIDEAYLDVTENKMGIKSAVKLAKMIQYDIWNDVHLTCSAGI
                     SYNKFLAKLASDFEKPKGLTLILPDQAQDFLKPLPIEKFHGVGKRSVEKLHALGVYTG
                     EDLLSLSEISLIDMFGRFGYDLYRKARGINASPVKPDRVRKSIGSEKTYGKLLYNEAD
                     IKAEISKNVQRVVASLEKNKKVGKTIVLKVRYADFETLTKRMTLEEYTQDFQIIDQVA
                     KAIFDTLEESVFGIRLLGVTVTTLENEHEAIYLDF"
     gene            19220..21532
                     /gene="pflB"
                     /locus_tag="VBE22_000780"
     CDS             19220..21532
                     /gene="pflB"
                     /locus_tag="VBE22_000780"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEO7202252.1"
                     /translation="MATVKTNTDIFEQAWEGFKGVDWKEKASIARFVQANYAPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEETRFPMDTRVASISELPAGFIDKDSELIFGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYATTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLAVYGADYLMQEKVNDWNALNDIDEESIRLREEI
                     NLQYQALGEVVKLGDLYGVDVRKPAMNTKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAYDALYSGDPTFITTSMA
                     GMGADGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYAFRRYCMSMSHKHSSI
                     QYEGVSTMAKEGYGEMSCISCCVSPLDPENEDKRHNLQYFGARVNVMKALLTGLNGGY
                     DDVHKDYKVFDIDPIRDEVLNFDTVKANFEKSLDWLTDTYVDAMNIIHYMTDKYNYEA
                     VQMAFLPSHVRANMGFGICGFANTVDSLSAIKYATVKPIRDEDGYIYDYETVGDFPRY
                     GEDDDRVDSIAEWLLEAFHGRLAKHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKG
                     VYLNEDGSVNLSKVEFFSPGANPSNKAKGGWLQNLNSLSKLDFAHANDGISLTTQVSP
                     RALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLKDVYDKIMNGEDVIVRISGYCV
                     NTKYLTPEQKTELTQRVFHEVLSMDDALTN"
     gene            21633..22007
                     /locus_tag="VBE22_000781"
     CDS             21633..22007
                     /locus_tag="VBE22_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO7202253.1"
                     /translation="MLNHKFLQVLKYEGVVSITSWTELAPHVTNTWNSYLTITDDQRI
                     LAPAAGMTHLENDLNNNSKIIMTLGSREVEGRDGYQGTGFRIEGTAKLLEAGSDFEIV
                     KKKYPFLRKVLEVTPINVIQLL"
     gene            complement(22042..22971)
                     /locus_tag="VBE22_000782"
     CDS             complement(22042..22971)
                     /locus_tag="VBE22_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO7202254.1"
                     /translation="MIKTNTVLELIKEQIKDNLYHGASLAIYENGEWHEHYLGTIDGN
                     EKVKAGLVYDLASVSKVVGVGTLLAKLVYQGTIDIDKPLRYYYPTFHHQTLTVRQLAT
                     HSSGIDPFIPNRDQLNATQLKDAINHIKVLEDKSFKYTDINFLLLGFMLEEVLGDSLD
                     KLFKRYIFTPFQMKETSFGPRVEAVPTVAGINDGIVHDPKAKVLGKHTGSAGLFSTID
                     DLQRFSIHYLKDDFAKPLWNNYSLSKSRSLAWDIDKDWINHTGYTGPFIALNYQKQAA
                     AIFLTNRTFSYDDRPLWIKKRRHVQEAITSYFE"
     gene            complement(22968..23723)
                     /locus_tag="VBE22_000783"
     CDS             complement(22968..23723)
                     /locus_tag="VBE22_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEO7202255.1"
                     /translation="MTLFHSLTFKHPVLRVNNRDLNIAFYQESLGFKLISEENAIAVF
                     SAWQNKEASFIIEESPTYRTRAVNGTKKLAKIIVKSQDAKDIEKLLANGAQTTQVYQG
                     QNGYAYETVSPEGDLFLLHAEDDLSQLVAIERPELEKNDDTTGLSNFVFQSISLNVPD
                     AVKAEAFYDKVFAGKFPINLSFKEAQGQDLQIAPNETWDIEILECCVNEDTNLNDLKS
                     TFESLGLDVYLDSKEKILVISDTSNIEIWISKE"
     gene            23848..24744
                     /locus_tag="VBE22_000784"
     CDS             23848..24744
                     /locus_tag="VBE22_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEO7202256.1"
                     /translation="MNETLLLHGIQLILIIAMIITFYQIVRHIRSQKINPFKRFFTGL
                     WIGFVTDALDTLGIGSFATTTTFFKLTKLVEDDRKIPATMTAAHVLPVLLQSLCFIFV
                     VKVEALTLITMAGAAFIGAFVGAKMTKNWHAPTVQRILGTLLITAAIIMLYRMITNPG
                     AGISDSVHGLHGIWLFVGIVFNFIIGVLMTMGLGNYAPELIFFSLMGLSPAVAMPVMM
                     LDAAMIMTASSTQFIKSGRVNWNGFAGLVTGGILGVIVAVLFLTNLDLNSLKTLVVGI
                     VLFTGAMLIRSSFKKTMPKSHH"
     gene            complement(24823..25671)
                     /locus_tag="VBE22_000785"
     CDS             complement(24823..25671)
                     /locus_tag="VBE22_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO7202257.1"
                     /translation="MIEITWTVKYITEFIATAFLIILGNGAVANVDLKGTKGNNSGWI
                     IIAIGYGLGVMMPALMFGNVSGNHINPAFTLGLAFSGLFPWAHVGQYILAQILGAMFG
                     QLVVVMVYQPYFVKTENPNHVLGSFSTISALDDGQKSSRKAAYINGFLNEFVGSFVLF
                     FGALALTKNYFGVELVGKLVQAGYDQTTAATRISPYVTGSLAVAHLGIGFLVMTLVAS
                     LGGPTGPALNPARDLGPRIVHRLLPKQILGQAKEDSKWWYAWVPVLAPIVASILAVAL
                     FKLLYL"
     gene            complement(25840..26292)
                     /locus_tag="VBE22_000786"
     CDS             complement(25840..26292)
                     /locus_tag="VBE22_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO7202258.1"
                     /translation="MTQKYERILIAIDGSYESELAVEKGINVALRNDAELLLTHVIDA
                     HAYQSEGVFSDYVFDRQEQESADVLAYFEKLAHSKGLTKIKKITEIGHPKTLLAKDIP
                     IREKADLIMVGATGLNTFERLLIGSTSEYILRHSKVDMLVVRDSKKTL"
     gene            complement(26310..27512)
                     /locus_tag="VBE22_000787"
     CDS             complement(26310..27512)
                     /locus_tag="VBE22_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7202259.1"
                     /translation="MEDKLFNKHFIGITILNFIVYMVYYLFTVIIAFIATKELGVSTS
                     QAGLATGIYIVGTLIARLIFGKQLEVLGRKLVLRGGAIFYLLTTLAYFYMPSIGVMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPADKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HINFKMVIVLCSILIAIVVLGAFVFPVKNITLNPEQLAKSKSWTIDSFIEKKAIFITI
                     IAFLMGISYASVLGFQKLYTTEINLMTVGAYFFIVYALVITLTRPSMGRLMDAKGDKW
                     VLYPSYLFLTLGLALLGSAMGSVTYLLSGALIGFGYGTFMSCGQAASIKGVEEHRFNT
                     AMSTYMIGLDLGLGAGPYILGLVKDGFLGAGVQSFRELFWIAAIIPVVCGILYFLKSS
                     RQVETKTI"
     gene            27668..>28253
                     /locus_tag="VBE22_000788"
     CDS             27668..>28253
                     /locus_tag="VBE22_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO7202260.1"
                     /translation="MNHQILQNYINSHNLPIIEKDYHKYLTFESLEEDYTYILKDGIV
                     KQSVLSKYGTEFNLRYVTGLEITSILNTDYSQHMGEPYNVRIESETAHFYKVRRSTFL
                     KDINNDIELQGYVKDFYHNRLEKSMKKMQCMLTNGRIGAISTQLYDLSKMFGEERDNG
                     DIYINFVITNEELGKFCGISTGSSVSRILKQLKDD"
BASE COUNT         9253 a         5438 c         4615 g         8947 t
ORIGIN      
        1 tacgcaatgt tagcttacaa aaaggaactg agtaatatta ctcagctccc aataaactat
       61 cttgctaact tctcttttaa ataatatcct gtataggatt taggattttc agcaacttct
      121 tctggtgttc cgatagctac aatttgtccg ccaccaatac ctccttcagg gccgagatca
      181 ataatgtggt cagcagtttt aataacatca aggttatgct caataacaag cactgtatta
      241 ccatcatcta caaaacgatc caaaacctta agtaagcgag caatatcatc tgcatgaagc
      301 cccgttgttg gttcgtctaa tatgtaaaga ctcttacctg tcgaacgctt atgtaattca
      361 cttgccaact tcattcgctg agcttctcct cctgataagg ttgtagctgg ttgccccaaa
      421 gtgacatatc ccaaaccaac atccttaata gtctgtaact tacgagcaat tttaggaata
      481 gctgcaaaaa atgtaactgc atcattcact gtcatatcaa gaatttgtgc aatatttttt
      541 tctttgtaat gtacttccag agtctcacta ttatagcgcg tgccgtggca tacttcacaa
      601 ggaacgtaga catctggtaa aaaatgcatc tcaattttga tgataccatc accagaacaa
      661 gactcacaac gtccaccttt gacgttaaat gagaagcgtc cttttttata acctcgaatt
      721 tttgcctcat tagtttgagc aaaaagatca cgaatatcat caaaaacacc tgtataggtt
      781 gcgggattag aacgtggggt ccgaccaatt ggactttggt caatatcaat caatcgatca
      841 acatattcaa tgccttctaa agaaacatat ttacctggtt tatctgagtt acggttaagc
      901 ttctgcgcaa cagctttttt caaaatacta ttaattaagg ttgattttcc agaacctgag
      961 acaccagtta cggcaataaa tttaccgaga gggaacttga catctaaatt ttgtaggttg
     1021 ttttctgccg cacctttgat ttctaaaaag cgaccattgc ctactcttcg ttcagatggc
     1081 acaggaatca ctttcttacc tgacaaatat tgtcctgtaa tagatttagt atttttagct
     1141 acttgcttcg gtgtgccaga ggccacaatc tcaccaccaa acgcaccagc gccaggtccc
     1201 acatcgatta accaatcagc tgccatcatt gtatcttcat catgttcaac aacaataagc
     1261 gtattaccca aatcacgcat tttcttaaga ctatcaatca aacggtcatt gtctcgctga
     1321 tgaagtccaa tggacggttc atcaagtaca taaaggacac ctgataaatt agacccaatt
     1381 tgagtagcca aacgaatacg ttgactctcg cccccggaaa gcgttcctgc agaacgtgac
     1441 agatttaaat aattaagccc aacattattt aagaaactta ggcggtcatg aatttctttg
     1501 ataattggtc gagcaattaa ttgttcattt gaagataaac gcaacgtttc taaaagttct
     1561 aaatggtctg ctatcgataa atctgagact tgcccaatat taagaccttc ttctccccca
     1621 actcttacgc aaagtgcttg atcattgaga cgatagccat gacaagtgtt acattttagc
     1681 tcgttcatat attctcgcat aacattgcgt gtgtaatcgc tattagtttc atggtaacga
     1741 cgattaatat tattgacaac cccttcaaaa ggcaaatcaa tatttctctc gcctccaaaa
     1801 tcgttaatat aatggaaatg gaactctcgt tcgcctgaac catatagggc taggtcctgc
     1861 tccgccttgc ttaatttttc aaatggcgta tccatatcaa caccaaattg agtcatagct
     1921 tgctctagca tcgttggata ataatttgat gaaattggat tccaaggaac taaggcccct
     1981 tctctaaggg tcttgctttt atctggaata accaaatcta tatccacttc taacttaatt
     2041 cctaagccat cacaggtcgt acaagaacca aagggagcat taaaagaaaa aaggcgtggc
     2101 tctaattcag gaacagtaaa accacattca ggacaagaat aatgctctga aaacaataac
     2161 tcattgccat ccattgtatc aataacgaca taaccatccg acaaacgtag agccgcttca
     2221 acggaatcaa ataagcggga acgaatgccc tctttattaa ttaatcgatc tactacaata
     2281 tcaatattgt gcatcttact ttttgataat tcaggcacct cagtaacatc gtgaatatca
     2341 ccatcaatac gaacacgaac gtaaccatct ttttgtattt tctcaaatgt tgatttgtgt
     2401 tgtccttttt ttcgacgtat aattggggct aaaatttgca tctttgttcg ctctggaagc
     2461 gctagtacct tatccacaat ttgctctaca gaagatgctg tgatggctcc gtgaccattg
     2521 atacaatatg gggttccaac acgagcataa agcaaacgta aataatcgtt aatttctgtt
     2581 acagtcccaa cagtagagcg aggattctta cttgttgttt tctgatcaat agaaatagct
     2641 ggactaaggc catcaataga atctacatca ggcttttcca tatttcccaa aaattggcgc
     2701 gcgtaagcag aaagactttc aacgtaacga cgttgtcctt ctgcatagat agtgtcaaag
     2761 gccaaactag acttacctga tcctgatagt cccgtaacga caactaactt gtctcgtggg
     2821 atgtcgacac ttatattttt taaattgtga gctcttgctc ctcttatcat taatttatct
     2881 tgcataatgt tgtgcctcag cactcctttt tgaatagtgt tgacttatta tatcaaattt
     2941 taataaagtc agtatttttt tagttttaaa tttcatagaa aaaagagatg aactaatacg
     3001 ttcatactct ttgttattat cttctttgtt gtggtgccac tggtgccatt gagctttgaa
     3061 tattatattt acgttgtaaa taaaagcgca aaccaaacga tgctaaagca ataataaaca
     3121 aaacaacctc aggaagttta ggattaatag ttgcaggtag aagaggtact actgcaaata
     3181 aggcaaacca aactaacata actagtgcca gtactgcgaa agatttaagc cagcctggtc
     3241 tttcagattt tggtttaccc atatggcgat aaatatagta ataagtaacg tacatggccg
     3301 caccaccacc aaaaccaaga gttaaaattg aaataagccc tgtattgaca ttagattgac
     3361 caaatagcaa cattaaacca ttcaaagcag ttactaagcc aagaaatagt aaggaagtat
     3421 ctaaccacat caaccaagga ttggtatttt tttgtacaga tactctagct ttatcctcag
     3481 gtcgctcagt gaatgaggct gcccaagtag ttggtgctcc taagagtgag cgtgcggtaa
     3541 ttcctttttt ctgattatct ataatctctg ggagatgttc ttctaaaata gctttgattt
     3601 ctgcatctga ttttccatca gctaaaagtt gattagttgc aatatgaaca aactcttgat
     3661 tctttttagt taagtcttgt aaagtcatgt gaactccttg attaatatac tattaaaacc
     3721 attttttacg aataaaataa aatacaacaa atgaactcat caaaaaggcc aaaaggacaa
     3781 catagataaa gccatgtgcg agaccattca aaggcatcca attattttga aagttcatcc
     3841 catacgccga aaaaataacc gttggaatat ccaatgtcat ggttactagg gctaaggttt
     3901 tcataatggt attttgatta ttaccgatga ttgaagcagt cgtttctgtc atggcattta
     3961 agacattttc atagatattt gccatttcaa tagcctgctg agtttcgatc aatgtatctt
     4021 caagcagatc ttcatcttca atgtattttt ttaaactact ggtactactg gtcaattttt
     4081 tgacgattcg ctcattaaat tttaaagagg cctttagata gacaatagat ttttccaatt
     4141 ccatcatgtc gatcaattgc tcatttcgag ttgcactctc tagttgtgct tcaattttat
     4201 cgctctgcct atcaatagta cgcaaagcct gtaagtataa ttcggcattg cgataaagca
     4261 attggaaaac aaaacgtgtc ttcataaagg tataaaaatt tttgacacgt cgtctataaa
     4321 agtgatcaaa cagcgtcaaa tgttctaggc aagtagtaat aacagcgttg tcagtaacga
     4381 taatgcccat aggaatagtc atataataac ttttgttatt gcgttcttca taggtaggaa
     4441 cgtcaacaat aactaaagta taatcatctt caaccgagat acgggaagtt tcttccacat
     4501 cgaggggagc acgtaaatca tcaatatcaa taccaaattg atcagctact tgttcggatt
     4561 cttcctggga gggattaacc agatttatcc aagcacctgg ctcgaatgtt tcaatttctt
     4621 taaattcaat tgccgttgaa agaaacattt gcttcatggc gattcctcct ttgttataaa
     4681 aaatccatac cttagtatta tacaggaaaa ccatttgttt tgcacggtta atacggtggt
     4741 atcctaaatt tttctattat ttgactcgta aataccaata ataatggtaa aaagaataaa
     4801 aaaatagccg ctagggctat tttatattaa tcttcattta cgtaaggcat aagagccata
     4861 acacgagcac gtttgattgc tgttgttact ttacgttggt ttttagctga agttcctgtt
     4921 acacgacgtg gtaaaatttt tccacgttct gaaacgaaac ggctaagaag ctctgtatct
     4981 ttgtaatcaa catattcaat cttgttagct gcgatataat caactttttt acggcgtttg
     5041 aatccgccac gacgttgttg agccatgttt atttctcctt tatagtttta aattctgtcc
     5101 atctattata ttagaatggt agatcatcat ctgaaatatc cattggattt gagttaccaa
     5161 acggactctc atcacgacta aaattaggtg tttgttgaga agaagctgaa taactattat
     5221 ttgacggagc attgttatag ccaccattat tataagagtt aggtgaacca ccttctcgtg
     5281 tagcacgact ttccaataat tggaaacttt ccgcaacaac ttctgttaca tagacacgtt
     5341 gaccttgttg gttttcataa ttacgtgttt ggatacgacc tgtaattcca accaaagcac
     5401 ctttttttgc ccagttagcc aagttttcag cttgttggcg ccaaataaca cagttaataa
     5461 aatcagcctc acgttcgcca gattgatttt taaaattacg attaactgca agtgaaaaag
     5521 tggctaccgc ttgattactt ggtgtataac gaagttctgc atcacgggtc atgcgaccta
     5581 caagtactac attattaatc ataaactacc ttcttagtct actttaacga tcatgtgacg
     5641 aagaatgtca ccgttgattt ttgaaagacg gtcaaactca ttaagagcta ctgcatcttc
     5701 agcttcaacg ttaacgatat ggtaaagtcc ttcagtgaaa tcttggattt cgtatgcaag
     5761 acgacgtttt tcccaatctt ttgattcaac aacagttgca ccgttgtcag ataagataga
     5821 atcaaaacgt gctaccaaag cgtttttagc ttcttcctca atgtttggac gaataatata
     5881 aagaatttcg tatttagcca ttgatatgtt cctccttttg gtctaatgac tcatgaattt
     5941 ttcatgagta agtgaggtta tgctcacaag atattattat agtagaaata agttaataat
     6001 gcaaggtttt tatgttttta tttccctgat atcatttaaa aacattttta tttttcttta
     6061 ttgaaatgtg ccttatatca aacctatgta gccattttct tttactgact tatgttcaat
     6121 ccccattcta taattcaatt aaaaaataaa aaggcgaggt ttacacctca ccttcaacat
     6181 attatttaaa gaattcttta ttggtttaag ttttcgttgg agaaaaattc agtgtctccg
     6241 attttatatt tttaaatatt aaaatactat ttagggagtc cctctttatt ttttattacc
     6301 tttggtaaca ccaaaacata cacgaacttt tattcatatg tgttaccatt ttacatcttt
     6361 catattgaga tatttgatta gcaatacctc cttgactaac aaaaattact aacacatcta
     6421 ggggtatacc ctagtttata tttgttcatg attacctcaa gcgagcatgc gctaatggta
     6481 atgaactatt accacattgt ctgtcttcct taaattatat aaggacttat gtgtcaccgt
     6541 tactgtagcg acctacatct aaacccaaca taagtgcccc tccaactatt tctaggaaat
     6601 gaagaaaact tcaaatcctt gtgactgttt gcaacatcaa tgcattgata acaattttag
     6661 tgtatcgcca ttcttatagc gacccatatt aaaaattaac ataagcgcta cctccattaa
     6721 ctaaagttag cttgcctacc aatctttctt ttggagacta gtctccattc ttatacctag
     6781 agatattgta atagatcatg gataacctcc ttgatttaaa ctttatcttt ataatttaat
     6841 tatagtgtaa acgcttcctt aagtcaagaa aaaaatattg atgcaacatt tttcttcatc
     6901 attaattacg atctagtcaa catacctctt tgttaaatcc tctttctttg atacaattaa
     6961 tctatgatag atttaaaaga ctatggaatt gagatgtggc ctgaagatag aatcgcttct
     7021 tttcgtcgca cacttttagg gtggtatgac caagaaaaac gtgatctccc ttggcgaaga
     7081 actactaatc catactatat ttgggtttct gaaatcatgt tgcaacaaac acaagttaat
     7141 acagtaatcc cttattataa acgttttcta gagtggtttc ctcaaataaa agatttggca
     7201 gacgctcctg aagaacaatt actaaaggca tgggagggac taggttatta ttctcgggtg
     7261 cgtaatatgc aaaaggctgc acagcaagtg atggttgatt ttggtggcat atttcctcac
     7321 acttatgatg atattgcttc attaaaaggg attggtcctt atactgcagg tgctatcgcc
     7381 agcatctcat tcaacttacc agagcctgcc gttgatggca atgtgatgcg tgtcatggct
     7441 cgtttgtttg aagttaatta tgacatcggt gaccctaaaa atcgtaaaat ttttcaagct
     7501 attatggaaa tactaattga ccctgacaga cccggtgatt ttaaccaagc acttatggat
     7561 ctgggtacag acatcgaatc cgctaagaca cctagacctg atgaaagccc tattcgtttc
     7621 tttaatgctg cttatttgaa tggtacttat agtaaatatc ccataaaaaa tcctaagaaa
     7681 aagcctaaac caatgcgaat tcaggctttt gtcattcgta accaaaatgg ccaatatcta
     7741 ctggagaaaa atactaaagg gagacttcta gggggttttt ggtcatttcc tattatcgaa
     7801 acgtcacctc tttctcaaca attagattta tttgatgaca atcaatccaa ccccataatc
     7861 tggcaaaccc aaaatgaaac tttccaaaga gaatatcaat taaagcccca atggactgat
     7921 aatcactttc ccaatattaa gcacactttt agtcaccaga agtggacaat agaactcata
     7981 gagggggtgg tcaaagcaac agatttacct aatgctcctc acctaaagtg ggcagcaatt
     8041 gaggactttt ctctgtatcc ctttgcaacg ccacaaaaga aaatgctgga gacctactta
     8101 aaacagaaaa atgcttaatc gcaagtgaac tatgacggtt cactttttgt ttacaattcc
     8161 tttacgccgc tgtaaagttt tcctcagatt atcttttaat accctataaa tagtggtcta
     8221 tccctaatgt caaatacatt tgatagaccc gttagcttat cattcttata ctaatattat
     8281 gaaaggagat atttatggaa attggtcagc aaattattcg ctaccgtaaa caacaagcgc
     8341 tctctcaaga agaattagcc gagaaagtct atgtaagccg tcagagcata tctaactggg
     8401 aaaatgacaa aacctaccca gacatctata gcctcttact cctaagtcaa atttttcaag
     8461 tgagcttaga ccaactaatt aaaggagaca ttgagaaaat gaaatatacc atcactcagg
     8521 ttgataaaaa gaattttgaa cgagatacca aagtaatggt aacattgatg atactattaa
     8581 tgatttcaag ttatccatta gtctatttct tagagtggct aggacttggc atttttgttt
     8641 tattatcgat aattactatg acttatgcta atcgtgtgga acgttttaag aaaaaatatg
     8701 atgtgcaaac ttataaggaa atcctagctg tttcaagtgg aaaattatta gacgaaatcg
     8761 aaaaacggga agaaagagca aaacttcctt atcagaaacc ccttattgtc actgtatttt
     8821 tcctcattac tgttgccacc ttttttgcaa gtcgttttat ttttacatgg ttgttccatt
     8881 taagttagta gataataaaa taaagctcag cgtgacgctg ggctttattt tatttaactc
     8941 aattcagcaa taatagcttt cagttggtct ttagtatgaa cacctgcaac ttgtttaacc
     9001 acttctccgt cttttttaaa catcaatgtt ggaattgaca taataccaaa ctgacgggca
     9061 gtttccggat tttcatcaac atccattttc aaaattttga gttcatcttc atcgatttct
     9121 tgagataact gttctaagat tggtgcttgc atacgacatg gaccacacca agttgcccaa
     9181 aaatcaatga gtactaaacc ttctttagtt tcttctacaa acgtagcatc tgttacttct
     9241 aatgccatct tttttctcca tttcatctta atgataagcc tattgtacaa gaatttagtt
     9301 gtttttgaaa ctatttgcta cgaactcgct aaataaataa acaaagccct aaaatcaagc
     9361 caataaggta acctacaata acatccttag gatagtgaat acctgctatc acacggcaaa
     9421 ttgccaatag aaccgataat attaaacaaa ctattccaaa gtaaacataa tatcgtaaca
     9481 aacacatact aatcatcgta gctgaaaaaa catgtctgct aggcattgac cttccctcag
     9541 tatccttata aattaaaggt ttaatattcc atttttcata tggtcgtgga aaatcaaggc
     9601 gtttcctaat gtaagacaaa gctataaagc caacagcagg gataattaat agccttaacc
     9661 ataattgaaa tgtcatccct tgccaaagag taaatatcag aaaagatgga tagagtatgt
     9721 acatcattcc tgtgactatt tttagtaaaa aatttaaaag aattattaac tgtggggatt
     9781 ttctaaaagg ttgagatagt ttggcataaa attgttcata tgattccata atttacccta
     9841 acgttacaat tgtggcgcca gatccccctg cattttgtgg ggcataagca aaatgcttaa
     9901 cgtgcttatt acgacgaaga tattttgtca ctccctcacg gataacgcct gtaccaatac
     9961 catgaatgat atcaacttgt cccatgttgt taagcaatgc ttgatcaata aaattatcta
    10021 actcttgcat agcttcttcg tatcttttac ctctaagatc aagtcgagct cttggtccag
    10081 aactatcagc ttttttaacc acattaatct gtttactttt gactttctgt tcttcttgga
    10141 ctctaacaag ggtaaattca tcttgtgtta atgtcatttt gataattccc acttgtgctt
    10201 cccaacgtcc atcttttaat tgactagtta aggtacctcg ctgtccatag ctagtcacta
    10261 taatatcatc accaattcta ggagcacgag ctgctttgat ttttttagcc ttatttaaga
    10321 ctttattttt tgataaatca acttgaggtg ctaatttttt tatttgtgcc ttagcatcta
    10381 taatttcgtg aggttttaat tggctcttat cattgagttt ttttaagata gtatcactct
    10441 cattcaaagc catatctaca atttcttgag cttcttgata gattttttct aactctttat
    10501 cgcgctcatg cgaaaattca ttatagagtt tcttaaccgc acgattgaat ttgaggtttt
    10561 cttgttcaat ttctttaata tgatccagtc ttctacgtgt ctcaagtgtc tgtgcctcta
    10621 actgttcaat aatacggtta acatctgagt cagaatctgt catctgctta gcttgtttaa
    10681 caataaatgg agctaaacca aggcgagaag caatttcaaa tgcatttgat cgtccaggaa
    10741 ctccttgcat aaagcgatac gtagggctaa gcgtttcggc atcaaattcc atgctcgcat
    10801 tctctacaaa atttgtctca atcccataag cttttaattc tggatagtgc gtggtcgcca
    10861 tcgttttgat attacttaac ctaaggtgtt ctaaaatagc catagccaaa ctagcacctt
    10921 cttgaggatc cgttcctgct cccagttcat caaagagaac taaactattg tggtcagcct
    10981 cgtttaaaat actaactatg tgcgtcatat gactagaaaa ggttgataga ctttgttcaa
    11041 tagattgctc atcgccaata tctgcaaaga tattgttaaa tactgcaatt ttactacctt
    11101 tatccgctaa tactggcaaa ccagactgtc ccattaattg tgctaaaccg agtgttttta
    11161 gcataatcgt cttaccacca gtattgggac cagtgatgac aattgcagtt aaatcttgat
    11221 caaaatgtaa gtcattagcc acagggttac ttaacagagg atgacgaaca ttgattaatg
    11281 ctaacgtgct gtcattagaa atctcaggta tcgtcgcctt attatcagac ataaaaagat
    11341 atttagccct tacaaaatca agatgcccaa gaatccatgc attatttcta atagtggcga
    11401 catggggtct taacaagtct gaaaatgcgt gtaaaatacg actttcttca tgacgttcgt
    11461 cagctctaag ctgcgttatc tcttcgttta gtgtaactac agcacgaggc tcaatataaa
    11521 cagtacttcc tgaagaagag atgtcatgaa ccacaccaga aatacgattc cgataagtat
    11581 tttttactgg taggacactt cgtccactac gactagcgat tagattctct gataaaagct
    11641 ctgctttttc cttaagcata tcctgtaaaa tctgacgaac ccgtcgttca ctgtttgtta
    11701 attgacgacg gatacgctct aattctggac tcgcaaaatg ttctaaaaaa ccaccatcat
    11761 tgatagcttg aaaagaccct tgcagattag ggaattgttc caaattttca aacaaacgat
    11821 ccaaagattg gaaagaaaca ttatccaaat cagaataaaa gtgaatcata tccgaagaac
    11881 tctgtaaaac ttttttgata accaaaagtt cttgaatatt aagatcagct gaaagctcta
    11941 agcgttttaa actctcagag attgaactta ggctgactat gccaaatgag tgatgttcaa
    12001 caaaaatctg ttccatgtca gaaatttcat taaaactttt ttctatttta ggagcctccg
    12061 tcatcggctc cagctctgat aattcttctt gtgattgttc tgtcttgaga taaggtaata
    12121 tcaattcctt aactttgtta aattctaact gttctaaaat cttgttattc atactcttca
    12181 ttatacctaa atttatcaca aaatgacaac gttaaaaagg ttggaactct tactccaacc
    12241 ttactaaaca ttctattcaa agacctaccg cccaagtcct taccaaaaac tgagagaaga
    12301 atggtaagtc gttaattaaa aatctaacaa acagactatt ccataacttt tcttgaacaa
    12361 atgacatagg gactgttgcc agaatagtca aacacatact cataaataag agacaaacca
    12421 gaacggctaa aaaaccacta ataatattat tttgtaagct atcaaaatgt tcaactggtg
    12481 ctaaatgtag taaaacacct agcaagcgac ctactaagta agataaactg tagataaaaa
    12541 caaaggcaac acctgcataa taaacatgac tcaattgaaa tttgctaata tctttaaaaa
    12601 agtaaacttc tactccttgt acaggattag aatacggtac ccataacgta atctgactag
    12661 ctaattcttg gtaaaattga ctagcaatca tcaaagatac cattgacatt aacacgtaaa
    12721 agacttgtaa aaaaatacct ctactgtagc caatataaaa atgccaaata acaataatca
    12781 gcaaaagcaa tgatagcatg attaatcctc actcactttt tctttaatgt ccgaaatcgt
    12841 tttggagcgt aacttattga gctcatcttc cattttttca atgtcaattt ctcgacttag
    12901 ttgcactgat aaagcattaa ttgccatgag aatagcaatc gtttcattat cagcattagg
    12961 taatttttct ttaatagctt catacttttc tgtagctaca cgctcaatct cttccataaa
    13021 aaggttgtct ttatctgtcg tcaatgtgag aggcttatct ccaaagacaa atttataacg
    13081 atttttctga ctattattta tttttgtttc catactatac ctctgctagc attataccgt
    13141 aaaatagggc tttcgtaaaa ttctacccct tatcattact tgtctattcc cttattcctc
    13201 ttttttatga tagaatattg tctatgaaca caatcgtcat gcaagcagat aaaaaattgc
    13261 aagaaaagat aagaaccgat ttagctcaac atcatataag taataataat ccttatgtgg
    13321 tattttcagc taaaatatca ggaactactg ttcttttata tacttctgga aaacttgtct
    13381 tccagggttc taacgcttca catatcgccc aaaagtatgg ttatattgag caaaaagaaa
    13441 gttgttcctc tgagactcaa gatattccca taattggtac tgatgaagtg ggaaatggct
    13501 cttactttgg tggactggca gtggtagcta gttttgtaac tcctaaggac catgcttacc
    13561 tcaaaaaatt aggtgttggt gattcaaaaa cacttactga tcaaaaaatt aagcaaatcg
    13621 cacctctttt ggaaaaagca attccacaca aagcattatt gttatcgcct caaaaatata
    13681 atcaagtggt tggtccaaat aacaaacata atgctgtctc cgtaaaagtt gctttgcaca
    13741 accaagctat cttccttctt cttcaagatg gttttgaacc agaaaaaatc gttattgatg
    13801 cttttactag ctccaaaaac tatcaaaact atttgaaaaa tgaaaagaat caatttaaac
    13861 aaacgattac tctggaagaa aaagctgaaa ataaatatct agctgtagcg gtcagttcta
    13921 ttattgcacg taatctattc ttagaaaatc ttaacaaatt aagcgacgac gtaggatata
    13981 aacttccaag tggagctggt caccaatctg ataaggtcgc tagccaatta ctcaaagctt
    14041 atggtatctc ttcgcttgaa cactgcgcaa aacttcattt tgccaacacc aaaaaagcac
    14101 aagcattatt aaaataaata gcagggaaaa ttatgaaaga atttattaaa gaatggggtg
    14161 tctttatcct catcctctca ctttttttac tatcgcgtat ctttttatgg caattcgtta
    14221 aagttgacgg acactccatg gatccaactt tagctgacaa ggaacagcta gtagttctca
    14281 aacaaacaaa aatcaatcga ttcgatattg tagtggctaa cgaagaagaa ggcggccaaa
    14341 agaaaaaaat tgttaaacgt gtcattggta tgccaggtga tgtcatcaaa tataaaaatg
    14401 acaccttaac tattaacaat aaaaaaacag aagaacctta cctcaaggaa tatactaaat
    14461 tatttaaaaa ggataaatta caggaaaaat attcgtataa cccacttttc caagacctag
    14521 cacaaagctc taccgctttc actactgaca gcaatggcag cagcgaattt actactgtcg
    14581 tgcctaaagg ccactattat cttgttggtg atgaccgaat tgtctctaaa gatagtcgtg
    14641 ccgtcggtcc cttcaaaaaa tcaacaattg tgggagaggt taaattccgc ttctggccaa
    14701 ttcgtcgttt tggaactatc aactaaagga aattaaaaat ccacctgaca aaagttgaaa
    14761 taacatatta tatttaaaat agggagtgac aaatccattt aaaataggga tttacaatca
    14821 ctccctttaa ctgtttatta aaaaggaagg tacaatggaa gtatttttta caggaaccat
    14881 tgaacgcatt atatttgaaa atgccagcaa tttttttaaa atattacttc tggaaatcga
    14941 agatacggat agcgattttg atgatgtaga agtaattatt actggtacta tggccgacgt
    15001 tatagaggga gaagaatata ctttttgggg aacactaacc caacatccaa aatacggcga
    15061 gcaactacaa agtgtacgct atgaaagagc caagcctact tcaggtggcc ttgtgaaata
    15121 cttttctagc gaacaattta aaggaattgg taaaaagact gcccaacgca tcgtagaact
    15181 ctatggagat aataccattg ataaaatcct tgaatcccct gaacagttaa gtacaatctc
    15241 aggactatca aaaattaata gagaagcatt catcgctaaa ctgaaactca attatggaac
    15301 agagcaagtt ttagctaaac tcgctgaata tgggttaagc aatcgtgctg ctattcaaat
    15361 atttgatcac tataaagaag aatcacttga agtgataaac gaaaatcctt atcaacttgt
    15421 tgaggatatc caaggtatcg gttttaaaat agctgaccaa cttgctgaac aagtcggtat
    15481 tgaaagtgat tctcctaaac gctttcgtgc tgctattatt catactttag ttgagtcttc
    15541 tatggagcaa ggagatacct atattgaggc tcgtactctc ttggaaaaaa ccattactct
    15601 ccttgaagaa gttcgtcaaa tcgaactaga tccttcaatt gttgctaagg aattgacaaa
    15661 tttaattgca gaagataaag tgcaacatat tggaactaaa atttttagta atactctatt
    15721 ttttgccgaa gaaggcatca aaaaaaactt acaacgaata ctaaatcagc ctcttgataa
    15781 acaactaagt cacaaagata ttgaccgtga aatcagagat attcaaaaat ctttaaatat
    15841 ccactacgat aatatccaag aaaaagctat tagagaagct ttactaagta aggtatttat
    15901 cttgactggt ggcccaggaa caggtaaaac aacagttata aatggtatta ttgaagccta
    15961 ttccgagtta catcatattg atttaaacaa aaatgatatt cctattgtac ttgctgctcc
    16021 tacaggtcgt gctgctagac gcatgaatga acttaccggt ttaccgagtg ctactattca
    16081 taggcatttg ggattaaacg gagatagtga ctatcaaagt ctagatgact atctagattg
    16141 ttccttaatt atcattgatg aattttcaat ggttgacact tggcttgcta atcaattatt
    16201 tgacgctttg gactcgcaca cacaagttat tattgtcgga gatagtgatc aattaccgtc
    16261 tgtaggtcca ggacaagttc tagcagatct tttaaatata aatgcacttc cacacattaa
    16321 acttgaaaag atttttagac aatcagaaga atcaactatt gtgactttag ctaatcaaat
    16381 gagacagggc tttttgccag aggactttac agctaaaaaa gccgatcgct catactttga
    16441 agcatctgct aatatcatcc caaatatgat ttctaaaatt gttcaatctg cacttaagag
    16501 cggtattgaa gctcatgaaa ttcaaatttt agctcctatg tatcgcggcc aagcaggtat
    16561 taataatctt aaccttatca tgcaaaatct cctcaatcca ttaaaagata ataaccaatt
    16621 tacttttaat gatataaatt tccgaatagg agataaagtg ttacatttag tcaacgatac
    16681 agagcttaat gtttttaatg gggatattgg ttacataacg gaccttatac cagctaaata
    16741 tactgagagc aagcaagatg agatttatat gacttttgat ggtcaagaag ttatctacca
    16801 acgcaaagaa tggcttaaaa taacactagc ctatgctatg agtattcata aatcacaggg
    16861 ttctgaattt caagttgtta ttctgccaat aacgagacag agtggtcgaa tgttacagcg
    16921 aaaccttatt tatacagcta ttacgagatc taaaagtaaa ctaatacttt taggagaaat
    16981 tggtgcattt gatttcgctg ttaaaaacga gggggcaaag cgcaatactt atctgataga
    17041 aagatttgaa aacaagcaag aaatagctaa ttctcaaaaa attgaagata gctcaataga
    17101 tcaaaagatt gacaacacaa taattaatac gtccattcca aaaacagcaa caccaattga
    17161 gcagactaat ctttcaaaaa taacttatcg cctaactgaa gaaaattatt taacaatcga
    17221 tccaatgatt ggtattaatc aacaagatat aagtgcaatt tttgatagta aataaatttt
    17281 ttcataaaat cttgaaaact cttccaaata tgttataata tcattgtatt aagaacgtag
    17341 ttcaaattta atacttttat taaggtttat ttagaaaaag gagatattat ggtttcatat
    17401 gaaaaagtcc gccgctctct gcgcacagct acaatcacta tcattgtatt aaatagtttg
    17461 tctttagtat tcagattgtt tacaggaatt tcagtgcagc tagccaaaac tgaaattaat
    17521 aaagggaaca caggtaatct tcctaaagaa catatcgaag cagtcttgag cgccacaaca
    17581 ccatttatgc tatttgtcac tgctttaata gtattagtca acattgccat tgttattttc
    17641 tgtattaaaa atttaagagc aattaaaaga aatcaaatgg ttaattacct tccttattat
    17701 cttggcttcg ccattactgt aggacttgtt attttgggtt ttctaactac taaagcacca
    17761 tgggcaatag ctataaatat cgtcttccaa gctattttcg gtcttcttta tttccatgct
    17821 tatcaaaaag cacaaaaact caacgaacgt gatcttgaag agactaacta aaagtaaaag
    17881 cactagaatc tagtgctttt ttcttttcaa agagagttaa atatagtcat cgtcccaact
    17941 tttaaaaatc taaatagata gcttcatgtt cattttccag ggtcgttact gtaacaccta
    18001 agagtcgtat gccaaaaact gactcttcta aggtatcaaa tatcgcttta gcaacttgat
    18061 caatgatttg aaaatcttga gtatactctt ctaatgtcat acgtttcgtc aatgtttcaa
    18121 aatccgcata tctaactttt aaaacgattg tcttaccaac tttcttattt ttctccagag
    18181 aagcaacaac tctctgaacg ttctttgaaa tctcagcttt gatatctgct tcgttatata
    18241 gtaatttccc ataggttttc tcacttccta ttgatttacg aactctatca ggttttaccg
    18301 gggaagcatt aatccctctt gccttacgat aaaggtcata cccgaaacga ccaaacatat
    18361 caattaaaga aatttcagat aaggaaagga ggtcctcacc agtataaacc cctaaagcat
    18421 gtaatttttc gacggagcgt ttaccaacac catgaaattt ctctattggt aagggcttca
    18481 agaaatcttg agcttgatct ggtaggatga gggtaagtcc ttttggtttc tcaaagtcag
    18541 aagccaattt tgccaaaaat ttattgtagg aaatacccgc tgaacaggtc aaatggacat
    18601 cattccaaat atcgtattgt atcattttag caagtttaac agcagatttt attcccattt
    18661 tattttcagt tacatctaaa taagcttcgt ctatactcat cggttcaacc aaatccgtat
    18721 actttttaaa tatatctcgt acttccattc ctacttggcg atatttttgg taatttcccg
    18781 aaataaagat agcttgaggg catcgttcat acgcttcttt tgaagacata gctgaatgaa
    18841 ctccaaactt tcgagcctca taattacaag tagaaacaac ccctctgccg ccggtttttc
    18901 tgggatcaga accaataata acaggctttc ctttaagaga gggattgtca cgctcttcca
    18961 cagatgcaaa aaaagcatcc atatcaatgt ggataatttt acgcgacgta tcatttatta
    19021 aaggaaaaat taacattgtt ctctccccca ttctctctac tccattataa cagattacaa
    19081 cttatgatac agattaaaaa aactgtatta taaaagaatc ataattttgt caagaaatat
    19141 gcttgcgaaa acgtttactt tttcctaaaa tggatatgta aacaaaatta aatctgtcac
    19201 aatgtttaag gagaaaacta tggcaacagt taaaacaaac acagatatct ttgaacaagc
    19261 ttgggaaggc tttaaaggtg ttgattggaa agaaaaagca agtattgctc gcttcgttca
    19321 agcaaactat gcaccttacg atggagatga aagcttctta gctggagcaa ctgaacgttc
    19381 acttcacatc aaaaaagtga ttgaagaaac aaaagctcac tatgaagaaa ctcgcttccc
    19441 aatggatact cgtgttgctt caatttctga attaccagct ggttttatcg acaaagatag
    19501 cgaactcatc tttggtattc aaaacgacga gcttttcaaa ttgaacttca tgcctaaagg
    19561 tggtatccgt atggcggaaa ctacacttaa agaaaatggt tatgaacctg atccagctgt
    19621 tcacgaaatc ttcactaagt acgctacaac agtaaatgat ggtatcttcc gcgcttatac
    19681 ttctaacatt cgtcgtgctc gccatgcaca cactgttact ggtcttccag acgcttattc
    19741 acgcggacgt atcattggtg tatatgctcg tctagctgtt tacggtgctg actacttgat
    19801 gcaagaaaaa gttaatgact ggaatgcatt gaatgatatt gatgaagagt caattcgtct
    19861 tcgtgaagaa atcaaccttc aataccaagc tcttggtgaa gttgtaaaac ttggtgacct
    19921 ttacggcgta gatgttcgta aaccagccat gaacactaaa gaagccattc aatgggtgaa
    19981 cattgctttc atggctgtat gtcgtgttat caatggtgct gcaacatcac ttggacgtgt
    20041 tcctattgtc cttgatatct ttgcagaacg tgacctcgct cgcggtacat tcacagaatc
    20101 agaaatccaa gaatttgttg atgattttgt ccttaaactc cgtactgtaa aatttgctcg
    20161 tactaaagct tatgatgccc tttactcagg tgacccaacc tttatcacaa cttcaatggc
    20221 aggtatggga gctgatggac gtcatcgtgt taccaaaatg gactatcgtt tcttaaatac
    20281 acttgacaat atcggtaact caccagaacc aaacttaact gttctttggt cagaccaatt
    20341 accatatgct ttccgtcgct actgtatgtc aatgagtcat aaacactctt caatccaata
    20401 tgaaggtgta tcaacaatgg ctaaagaagg ctacggtgaa atgtcatgta tttcatgttg
    20461 tgtatctcca cttgatccag aaaacgaaga caaacgtcac aaccttcaat attttggtgc
    20521 tcgtgttaac gttatgaaag cacttcttac tggtcttaac ggtggttatg atgatgttca
    20581 taaagattac aaagtatttg acatcgaccc aattcgtgac gaagtactca actttgacac
    20641 tgtaaaagct aactttgaaa aatctcttga ctggttgact gatacttatg ttgatgcgat
    20701 gaatatcatc cactacatga ctgataaata caactatgaa gctgtacaaa tggcattctt
    20761 accttcacat gtacgtgcta atatgggatt tggtatctgt ggttttgcaa atactgtcga
    20821 ttcattatct gcgattaaat acgctactgt taaaccaatc cgtgacgaag atggttacat
    20881 ctacgactac gaaacagttg gtgacttccc acgttatggt gaagatgatg atcgtgttga
    20941 ctcaatcgct gaatggttac ttgaagcctt ccatggacgc cttgctaaac acaaacttta
    21001 taaagatgct gaagcaactg tttctcttct tacaatcaca tcaaacgttg cttactctaa
    21061 acaaactggt aactcaccgg tacataaagg agtatatctc aatgaagatg gtagtgtaaa
    21121 cctttctaaa gtagaattct tctcaccagg cgctaaccct tctaacaaag ctaaaggtgg
    21181 ctggttacaa aacttgaatt cactttctaa acttgatttc gctcatgcaa atgatggtat
    21241 ttcattgaca actcaagttt ctccacgcgc tcttggtaaa acatttgatg aacaagttga
    21301 taacttggta acagtccttg atggttactt tgaaaacggt ggtcaacacg ttaacttgaa
    21361 tgtcatggac cttaaagatg tctatgacaa gattatgaat ggtgaagatg ttatcgttcg
    21421 tatctcaggt tactgtgtca atactaaata tctaactcct gagcaaaaaa ctgagttaac
    21481 acaacgtgtc ttccatgaag tactttcaat ggacgatgca ttaactaact agtttgtatt
    21541 cgatgaagta ggtttgatta tatcaaacct acttttatct atattatcaa tagtactaat
    21601 tacttcttta tataacctca aggaggggac taatgttaaa tcacaaattt cttcaagttc
    21661 taaagtatga gggtgtcgtt tcgataacaa gctggactga attagctcct cacgttacaa
    21721 acacttggaa tagttacctg actattacag atgaccagcg tatcttagct cctgcagcag
    21781 gaatgactca tcttgaaaat gatctcaata ataattctaa aattattatg actctgggca
    21841 gccgtgaagt cgagggtcgt gatggttatc aaggtacagg ctttagaatt gaaggaactg
    21901 ctaagctatt agaagcagga agcgattttg aaattgttaa aaaaaaatac ccttttctgc
    21961 gtaaagtatt ggaagtaact cccataaatg ttatccaact tttataaaaa acttctccta
    22021 gaattttcta ggagaagttt tttattcaaa gtacgatgtt atcgcctctt gtacatgtcg
    22081 tcgtttctta atccaaagtg gacggtcatc ataggaaaag gttctgtttg tcaaaaaaat
    22141 agctgctgcc tgcttttgat aatttagtgc aataaaagga ccagtataac cagtatggtt
    22201 tatccaatcc ttatctatat cccaagctaa tgatcttgat ttactcaaac tataattatt
    22261 ccacaaaggt ttcgcaaaat catcttttaa ataatgaata gaaaatcttt gtaaatcatc
    22321 aatagttgaa aataaacctg cagaaccagt atgctttcct aataccttag ctttaggatc
    22381 atgtacaata ccatcgttaa taccagctac agttggaaca gcttctactc taggaccaaa
    22441 agaggtttct ttcatctgaa aaggagtaaa aatataacgt ttgaaaagtt tatccaaact
    22501 atcccctaat acctcttcta acataaaacc aagtaaaaga aaattaatat cagtgtattt
    22561 aaacgattta tcttccaaaa ccttgatatg attaatggca tctttcagct gagtagcatt
    22621 caactgatca cgatttggaa taaaaggatc aatcccactg ctatgtgtag ctagttgcct
    22681 tacagttaac gtctgatgat gaaacgtagg gtaataatac cttaatggct tatcaatatc
    22741 aatagtccct tgataaacta actttgctag taaagttccc acgccgacaa cttttgatac
    22801 actggctaga tcatagacca atcccgcttt aactttctca ttgccatcaa ttgtccccaa
    22861 gtaatgttcg tgccactctc cattttcata aatagctaag cttgcaccat gataaagatt
    22921 atctttgatt tgctctttaa tgagttctaa aacggtatta gttttaatca ttcttttgaa
    22981 atccaaatct caatattact agtgtcagag atgactaaaa tcttttcttt agaatcaagg
    23041 taaacatcaa gtcccaatga ttcaaaagtt gatttcaagt cgtttaaatt agtatcttca
    23101 ttaacacagc attctaatat ctcaatatcc caagtttcat ttggagcaat ttgcaaatct
    23161 tgtccttgtg cttctttgaa agataaatta attgggaact tcccagcaaa tactttatcg
    23221 taaaaagctt cagctttcac ggcatcaggt acattcaaac taatagattg gaaaacaaag
    23281 tttgatagac ctgtagtgtc atcatttttt tctaactctg gacgctcaat tgctactaat
    23341 tgtgacaaat catcttcagc atgaagtaag aaaaggtctc cctcgggcga aactgtttca
    23401 taggcataac cattttgacc ttgatatact tgagtggttt gcgcaccatt tgccaaaagc
    23461 ttttcaatat cttttgcatc ttgagactta acaataatct ttgctaactt cttagtacca
    23521 ttcaccgcac gagtacgata agttggggat tcctcaataa taaagctagc ctctttgttc
    23581 tgccatgctg agaaaactgc gattgcattt tcttcactta tcaatttaaa tccaagagac
    23641 tcctgataaa atgcaatatt caagtctcta ttattaacac gtaatactgg gtgtttaaat
    23701 gtcaatgaat gaaataacgt cataaattcc tccttgttca gtctagtcta ttttagataa
    23761 ttttagataa tttagcaaga atctttgtgc tctcaatacc ataaagtcaa attgacagtt
    23821 tcactatttt tttgtagact ggatattatg aatgaaacac tactattaca tgggattcag
    23881 cttattctga ttatcgccat gattatcact ttttatcaga ttgttagaca tattaggtct
    23941 caaaaaataa atcctttcaa acgatttttc actggtttgt ggatcggttt tgtaactgat
    24001 gctctcgata ctcttggtat cggctctttt gcaacaacga ccacattttt taaactaact
    24061 aagcttgttg aagacgatcg taagataccc gcaacaatga cagctgcaca cgtacttcca
    24121 gttttgttgc agtcactctg ttttattttt gttgtaaaag tagaggcttt aacactaata
    24181 acaatggctg gtgcagcttt tattggggct tttgttggag ctaaaatgac taaaaattgg
    24241 cacgctccta ctgttcaacg tattctagga acactgctca tcacagctgc tattattatg
    24301 ctctatcgta tgatcactaa ccctggcgct ggtatttcag attcagtgca tggtctacac
    24361 ggcatttggt tatttgttgg tatcgtcttc aattttatta ttggtgtatt aatgactatg
    24421 ggactaggta attatgctcc tgaacttatc ttcttctccc taatgggact gagtcctgca
    24481 gttgctatgc ctgtcatgat gctagatgca gctatgataa tgacagcttc tagtacacaa
    24541 tttatcaaga gtggtcgtgt taattggaac ggctttgccg gtctggttac tgggggaata
    24601 ttaggcgtta ttgtcgctgt tctcttctta actaaccttg accttaacag cttgaaaaca
    24661 ctggttgtag gtattgtttt atttactgga gcaatgttaa tacgttcttc ctttaaaaaa
    24721 actatgccca aaagtcatca ttaaacaaaa tcagctagca agactggctg atttttatat
    24781 ttaaaacaaa aaaagaccgg taaaccgatc tttccttaac tattaaagat ataatagttt
    24841 gaacaaggct acagcgagta tacttgctac gattggtgct aaaactggca cccatgcata
    24901 ccaccatttt gaatcttctt tagcttgtcc aagaatttgt tttggtaata agcggtgaac
    24961 aatacgtgga ccaaggtcac gcgcagggtt caatgcaggt ccagtcggtc cacctaaaga
    25021 agcaactaga gtcattacca agaaaccaat acctaagtga gctacagcaa gtgaaccagt
    25081 tacgtatggt gaaatccttg tcgctgctgt cgtttggtca taaccagctt gaacaagttt
    25141 accaacaagt tctacaccga aatagttctt cgttaaagcc aaagctccaa agaaaagaac
    25201 aaaagaacca acgaattcat ttaagaaacc attaatgtaa gccgctttac gacttgattt
    25261 ctgtccatca tctaatgcag aaatagttga aaatgatcca agaacatgat ttggattttc
    25321 tgtcttaaca aagtaaggtt gataaaccat aacgacaact aactgtccaa acattgctcc
    25381 caaaatttgt gcaagaatgt attgtccaac gtgtgcccaa gggaaaagac ctgaaaatgc
    25441 caatccaagt gtgaaagctg ggttaatatg gtttccagag acattaccaa acataagcgc
    25501 tggcatcata acacctaaac cataaccaat tgcaataata atccaacctg aattatttcc
    25561 ttttgtgcct tttaagtcaa cgttagctac agccccattt cctaagatga taagaaaagc
    25621 ggttgcgata aattctgtga tatacttcac agtccaagtg atttcgatca atccttgttt
    25681 cctcccaaaa aaatatttac aacattaacc attctaccaa gtaagcttaa tattgtaaag
    25741 cttatcacaa aataaaatta ataaaatttt taaccaaata tatttgataa aaaaatagag
    25801 tactaggttc ggactaccta gtactctatt ttatttagtt tataaggttt tcttactatc
    25861 acgaacgact agcatatcaa ctttcgaatg acgtaaaatg tattctgaag ttgagccgat
    25921 caatagtctt tcaaaagtgt ttaatcctgt tgcgcctacc ataattaaat ctgctttttc
    25981 ccttatgggg atatcctttg ccaaaagagt cttaggatgt ccgatttcag taattttttt
    26041 gatctttgta aggccttttg agtgtgccaa tttctcaaaa taggctagca catctgctga
    26101 ctcttgttct tgcctatcaa aaacatagtc actaaagact ccctcacttt ggtaagcgtg
    26161 agcatctata acatgcgtta gcagtaattc agcatcatta cgaagagcaa catttatccc
    26221 tttttctacc gccaattctg actcgtaaga cccatcgata gcaataagaa tacgctcata
    26281 tttttgtgtc atagcttaat tcctcccttt tatatagttt tagtttcaac ttgtcgagat
    26341 gattttaaga aatatagaat accacaaaca acaggaataa tcgctgctat ccagaataat
    26401 tccctaaagg attgcacacc agctccaaga aaaccatctt taacaagtcc caaaatgtaa
    26461 ggtccagcac ctaaccctaa atcaagacct atcatgtaag ttgacatggc tgtattgaaa
    26521 cgatgttcct caacaccttt gattgatgct gcttggccac aagacataaa ggtgccataa
    26581 ccaaaaccaa tcaaagcacc tgatagaagg taggtaacac ttcccatagc actccctaat
    26641 aaagcaagtc ccaaagttaa gaacagataa cttggataaa gcacccactt atctccctta
    26701 gcgtccatta atcttcccat agatggtctg gttaaagtga tgacaagtgc ataaacaata
    26761 aagaaataag ctcctactgt catcaaatta atttctgttg tatataattt ttggaaacct
    26821 aacacggaag cataggagat acccatcaaa aatgcaataa ttgtgataaa gattgctttt
    26881 ttctcaatga aactatcaat agtccatgat tttgatttag ctaactgttc tggatttaaa
    26941 gtaatatttt tgactgggaa aacaaatgct cccaacacta caatcgcaat taaaatacta
    27001 cataatacaa taaccatttt aaagttaata tgaaggttgt ctagcataaa tgttcctaca
    27061 aaaggaccaa tagctgcggc taaacttgtt gatagaccgt aaaaattaat cccttcacct
    27121 cttttatcag ctggtatata ggctgttaca atagtattag ttgctgttga cacgacacca
    27181 taaccaaaac catttaggaa acgaactaaa tacattactc cgatacttgg catataaaaa
    27241 taagctaaag ttgttagtaa gtaaaaaata gcccctccac gtaaaactaa cttacgtcct
    27301 agaacttcta attgcttacc aaatataaga cgagcaatca aagtccctac aatataaatc
    27361 cccgttgcta atcccgcttg actagtgcta acacctaact ctttagtcgc aataaaagct
    27421 atgataacgg taaacaaata atagaccata taaacaataa agtttaaaat agtaatgcct
    27481 ataaaatgtt tgttgaataa tttgtcttcc aatgcaaatg gctccttttt tcaatttcat
    27541 tttcataaac attttacaaa aatattagaa gatacgatat ttcaatggta taatataagt
    27601 gctagtatca gactgcatta acaaatgtta aggtggtcta tcaaacctaa aacatttgga
    27661 ggaagtcatg aatcatcaaa ttttacaaaa ttacattaat agtcataatc ttccaattat
    27721 cgaaaaagac taccataaat accttacatt tgaaagttta gaagaagact atacttatat
    27781 cctaaaagat ggtattgtta aacaaagtgt tctctctaaa tatggtactg agttcaatct
    27841 gcgttatgtg acaggcttag aaatcacttc tatcctaaat acagactact cccaacacat
    27901 gggagaacct tacaacgttc gaatcgaatc cgaaactgcc cacttttaca aagtacgtcg
    27961 ctctaccttc ctaaaagata tcaataatga tattgaactg caaggatatg tgaaagattt
    28021 ctatcataac cgccttgaaa aatctatgaa aaaaatgcaa tgtatgctca ctaatggtcg
    28081 aattggtgct atctccacac aattatatga cctcagtaag atgtttggtg aagaacgaga
    28141 taacggtgat atctatatta attttgttat cacgaacgaa gaacttggta aattttgcgg
    28201 tatatccaca ggaagtagcg tgagccgcat tttgaaacaa ctaaaagacg atc
//
LOCUS       DATRTN010000017        26335 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.017, whole genome shotgun sequence.
ACCESSION   DATRTN010000017 DATRTN010000000
VERSION     DATRTN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26335
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.017"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(95..979)
                     /locus_tag="VBE22_000789"
     CDS             complement(95..979)
                     /locus_tag="VBE22_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7202261.1"
                     /translation="MDIRQLTYFIAVAEAKNYSHAAKSLFVTQPTLSQSIKKLEAELN
                     TILFLQNGRQLALTEAGEILYEKGQLLMTNFNQMVTEIQQLNQEKKEGIRVGLTSLFA
                     IQFMKQISTFMATHSNVEVSLIQDGSRKLQELLAKGKIDIGLLSFPSTRNDITIEPLQ
                     TSTKGYKVSIVMPKSHPLATLPEIELNDLRDYKVASLNEHYMLGEMLPRKCRALGFDP
                     HIVFKHNDWEVLIHSLQDLNAVTILPSEFESISQVQDLCWVPLKDKNNFYPIGIAYRN
                     DTSFSPIIEEFLSLLKTN"
     gene            1172..2230
                     /locus_tag="VBE22_000790"
     CDS             1172..2230
                     /locus_tag="VBE22_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO7202262.1"
                     /translation="MSDVVEKQTAKSFIMNVLNGLALGTVIVLIPGAILGELMKALLP
                     MWSGFATLIAATAVATSMMGLVIGIMVGLNFKFNPIQSASLGLAVMFAGGAATFLKGA
                     IMLKGTGDIINMGITAALGVLLIQFLSDKTKSFTLIVIPTVTLLLVGGVGHVLLPYVK
                     MITTMIGQGIASLLGLQPVLMSILIAMIFCFLIVSPITTVGIALAISLSGIGSGAANL
                     GICAASFGLCMAGWSVNSKGTALAHVLGSPKISMANVLAKPKIMLPMISSAAILGILG
                     ALFNIQGTPASAGFGISGLIGPINALNLAKGGWSVMNMLLIIIIFVAAPIILNFIFNY
                     LFIKVLKIIDPMDYKLDI"
     gene            2244..3236
                     /locus_tag="VBE22_000791"
     CDS             2244..3236
                     /locus_tag="VBE22_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEO7202263.1"
                     /translation="MKLKVFNVREEEATLAQDWANRNHVELSMSEGPLTLETVNEVEG
                     FDGIANAQIGPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLELAKQHGIIISNVPSYSP
                     ESIAEFTVTIALNLIRKVELIRANVREQNFSWTLPIRGRVLGNMTVAIIGTGRIGLAT
                     AKIFKGFGCRVIGYDIYHNPMADGILEYVNSVEEAVEKADLVSLHMPPTAENTHLFNL
                     DMFKQFKKGAILMNMARGALVETKDLLEALDQGLLEGAGIDTYEFEGPYIPKNCQGQD
                     ISDKDFLRLINHPKVIYTPHAAYYTDEAVKNLVEGALNACVEVVETGTTTTRVN"
     gene            3442..4992
                     /locus_tag="VBE22_000792"
                     /pseudo
     CDS             3442..4992
                     /locus_tag="VBE22_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            5059..6084
                     /locus_tag="VBE22_000793"
     CDS             5059..6084
                     /locus_tag="VBE22_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO7202264.1"
                     /translation="MAKIISLGEVLLRLSPPQYHTLMQANHLKCQFGGSELNVLASLA
                     QLGHHVGLVSALPDNDLGKMARQFILSQQISPAAIIKKEGRLGIYYYEQGFSVRTNKV
                     IYDRNYSSFWESTLSDYDFTSIFKGVDWFHVSGITPALTKDLYEVTRFLMTKAKEGGV
                     KVSIDLNFRESLWSSFQEAREQLSPLLGLSDVCFGLEPIYLAGESEDLKDELGLSRPY
                     LDIELLEKITQKIVQEYGLDYIAFTQREMGYTNQYMLKSYLYHNNMLYQTDKTGVEVL
                     DRVGTGDAFAAGLIHALLEKETPQRALEIAMTTFKYKHTIQGDINIMTRDDIAYLIEK
                     ETNDIKR"
     gene            6101..7900
                     /gene="uidA"
                     /locus_tag="VBE22_000794"
     CDS             6101..7900
                     /gene="uidA"
                     /locus_tag="VBE22_000794"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEO7202265.1"
                     /translation="MLYPLLTKTRNTYDLGGIWNFKLGEHNPNELLPSDEVMVIPTSF
                     NDLMVSKEKRDYIGDFWYEKVIEVPKVSEGEEMVLRFGSVTHQAKIYVDGILVGEHKG
                     GFTPFEVLVPECKYNNEKIKVSICANNVLDYTTLPVGNYSEIIQEDGSIKKKVRENFD
                     FFNYAGVHRPLKLMIRPKNHISDITITSRLSDDLQSADLHFLVETNQKVDEVRISVFD
                     EDNKLVGETKDSRLFLSDVHLWEVLNAYLYTARVEIFVDNQLQDVYEENFGLREIEVT
                     NGQFLLNRKPIYFKGFGKHEDTFINGRGLNEAANLMDLNLLKDIGANSFRTSHYPYSE
                     EMMRLADRMGVLVIDEVPAVGLFQNFNASLDLSPKDNGTWSLMQTKAAHEQAIQELVK
                     RDKNHPSVVMWVVANEPASHEAGAHDYFEPLVKLYKDLDPQKRPVTLVNILMATPDRD
                     QVMDLVDVVCLNRYYGWYVDHGDLTNAEVGLRKELLEWQDKFPDKPIIITEYGADTLP
                     GLHSTWNIPYTEEFQCDFYEMSHRVFDGIPNLVGEQVWNFADFETNLMILRVQGNHKG
                     LFSRNRQPKQVVKEFKKRWMTIPHYHNKKNSVK"
     gene            7929..8600
                     /locus_tag="VBE22_000795"
     CDS             7929..8600
                     /locus_tag="VBE22_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEO7202266.1"
                     /translation="MARPLVEQTADRLLHLILEREYPVGAKLPNEYELAEDLDVGRST
                     IREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLIKDTDRLTSDLFELRLLLE
                     PRIAELVAYRITDDQLQLLEKLVGDIEDAVHAGDPKHLLLDVEFHSMLAKYSGNIAMD
                     SLLPVINQSIHLINANYTNRQMKSDSLEAHREIIKAIREKNPVAAHDAMLMHIMSVRR
                     SALKA"
     gene            8717..9334
                     /gene="eda"
                     /locus_tag="VBE22_000796"
     CDS             8717..9334
                     /gene="eda"
                     /locus_tag="VBE22_000796"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO7202267.1"
                     /translation="MLNQLKNNYFFAVIRGKSSEDALEIAKHAILGGIRNIEVTFSTP
                     EASKVIKQLSDDFKNNKKIIVGAGTVMTTELAKDAIDAGAKFLVSPHFDSDIANLANE
                     NKVYYFPGCATATEIVVARKYKCQIIKLFPGGVVGPDLIKDIHSPIPDVDLMPSGGVS
                     VSNVAEWRKAGAVAVGVGSALSSKVATEGYDSVTKIAKQFVSALD"
     gene            9351..10751
                     /gene="uxaC"
                     /locus_tag="VBE22_000797"
     CDS             9351..10751
                     /gene="uxaC"
                     /locus_tag="VBE22_000797"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEO7202268.1"
                     /translation="MTFNTETFMLKNQAAIQLYEEVKRQPIFDYHCHLDPKDIFEDRI
                     FDNIVDLWLGGDHYKWRLMRANGISEAEITGPASNLEKFKSFARTLERAYGNPVYHWS
                     AMELKNVFGVNEILTESNAEEIYHRLNHFLKEHKISPRRLIADSKVMFIGTTDHPLDT
                     LKWHKKLAADESFKTVVAPTFRPDEAFIEHRHFVDFITKLGDITQKEITDFSTFIAAM
                     EERIAYFAQNGCRASDISFTEIVFEQTDELELNDLFNKVCEGYIPNQSEISKWQTAVF
                     MELCRLYKKYGFVTQVHFGALRNNHSTIFEKLGADVGVDSLGDQVALTVNMNRLLDSL
                     VKKDSLPKMIWYNLNPAYNIAVANTLANFQANELGVRSYLQFGAGWWFADTKLGMISQ
                     MNALAEQGMLANFIGMLTDSRSFLSYQRHDYFRRILCTYLGEWIEEGEVPEDYQALGS
                     MAKDIAYQNAVNYFKN"
     gene            10769..11815
                     /gene="uxuA"
                     /locus_tag="VBE22_000798"
     CDS             10769..11815
                     /gene="uxuA"
                     /locus_tag="VBE22_000798"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEO7202269.1"
                     /translation="MEMSFRWYGEDDPVTLENIGQIPTMKGIVTAIYDVPVGEVWSRE
                     RIQQLKEKVEAAGLKISVIESVPVHEDIKLGRPTRDLLIDNYIQTVKNLAAEGIDTIC
                     YNFMPVFDWTRTDLAYQYPDGSTALIFDETVSKKMDPVNGELSLPGWDASYSKEEMKA
                     IMDAYAEIDEEKLWENLTYFIKRIIPEAEAVGVKMAIHPDDPPYSIFGLPRIITGLEA
                     IERFVKLYDSKSNGITLCVGSYASDPQNDVLEISRRAFELDRVNFVHARNIKLGDGKS
                     FKESAHPSEYGSIDMYEVIKLCHEFGFEGAIRPDHGRMIWGETGRPGYGLYDRALGAT
                     YLSGLYEAVIKGSK"
     gene            11939..12778
                     /locus_tag="VBE22_000799"
     CDS             11939..12778
                     /locus_tag="VBE22_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO7202270.1"
                     /translation="MSKIITFTNKVVVITGAGGVLCGYMAKEFAKAGAKVALLDLNQE
                     AAQTFADEIVAEGGIAKAYKANVLSKENLEEVHQAVLEDLGPTDILVNGAGGNNPKAT
                     TDNEFHELDLPSETKTFFELDEAGISFVFNLNYLGTLLPTQVFAQDMVGREGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRSLLF
                     TEDGQPTARAEKILNNTPMGRFGEASELIGGLFFLADEKSSSFVNGVVLPIDGGFAAY
                     SGV"
     gene            12931..13743
                     /locus_tag="VBE22_000800"
     CDS             12931..13743
                     /locus_tag="VBE22_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7202271.1"
                     /translation="MVDTNHKIAFCIDSDGCAMDTMTYKHQLFFGPIAADIFGISEQE
                     RTSFLKEWDRVNLYSRTRGVNRFVGLVMGLEFAGVEHIDKLKEWVETTDSLSNNSLEL
                     ALANDFSRDLEKALEWSNEVNHQIKNYEGDSLAFSGVLESLERLSQFGNVYVVSSANK
                     EAVWQEWEEQGLLKYVDDIYCQDRGKKEDVIATLIASGYCRETILMVGDSPGDLAAAE
                     QNGIAFYPVLVGKEVKSWEILREDIGEAFAKGQFEQQRQKESINTFWANLDN"
     gene            13759..15549
                     /locus_tag="VBE22_000801"
     CDS             13759..15549
                     /locus_tag="VBE22_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEO7202272.1"
                     /translation="MTHLVDLTKKPFNLNQEAIEWIEKTINEMTLDEKIGQLFFNMGA
                     SRSEEYLTDVLDRYHIAAVRYNRGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAV
                     TDGTKVGDEIKVAATNDPKYAYEMGRIAGMEASAVGCNASFAPIVDLTRNWRNPIIAS
                     RNWGANVDQIISLSKEYMKGIMQYNIVPFAKHFPGDGIDERDHHLSFASNPMSKEEWM
                     STFGRIYGELADAGLPGVMAGHIHLPNVEKEMHPERDLDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMVGMTASMPRRDLLPTAIEAGCDLFLFFNDPDEDIQWMKEGYEKG
                     ILTEERLHDALRRTLGLKAKLGLHNYEGRRQELFMPKDKAMALINTLESQKIADEVAD
                     KAVTLVKDKQKDIFPVNPERYRHILLVNVEGYKGGFGAMIAGNKQRASDYMKELLEAR
                     GHEVTVWESTEERIMKLPQEERAAAIANVYAQKQPIANLTEHYDLIINLVDVNAGGTT
                     QRIIWPAAKGTPDQPFYVHEIPSIVISVQHAFALADMPQVGTYINAYDGLPSTISAVV
                     AKLAGESEFTGVSPVDAYCGLIDTHLWRQC"
     gene            complement(15607..16692)
                     /locus_tag="VBE22_000802"
     CDS             complement(15607..16692)
                     /locus_tag="VBE22_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO7202273.1"
                     /translation="MSKLNRIRHHLHSVQAELAVFSDPVTVNYLTGFFCDPHERQMFL
                     FVYEDRDPILFVPALEVSRAKQSVPFPVFGYIDSENPWQKIASNLPSFSVSKVLAEFD
                     NLNVTKFQGLQTVFDGHFENLTPYIQNMRLIKSRDEIEKMLVAGEFADKAVQVGFDNI
                     SLNNTETDIIAQIEFEMKKQGINKMSFDTMVLTGNNAANPHGIPGTNKIENNALLLFD
                     LGVETLGYTSDMTRTVAVGKPDQFKKDIYHLCLEAHQAAIDFIKPGVLASEVDAAARN
                     VIEKAGYGQYFNHRLGHGLGMDVHEFPSIMAGNDMEIQEGMCFSVEPGIYIPDKVGVR
                     IEDCGYVTKTGFEVFTKTPKELLYFEG"
     gene            16902..17906
                     /gene="ccpA"
                     /locus_tag="VBE22_000803"
     CDS             16902..17906
                     /gene="ccpA"
                     /locus_tag="VBE22_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEO7202274.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILARGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPIVLAGTVDLEHQLPS
                     VNIDYKAAAVDVIDILAGNHKDIAFVSGPLIDDINGKVRLAGYKEGLKKNGLNFKEGL
                     VFEANYRYAEGFALAQRVINAGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPIAQYTRPNLTSISQPVYDLGAVSMRMLTKIMHKEELEEKEIVLNHGIVKRGTTNN
                     "
     gene            18038..19504
                     /locus_tag="VBE22_000804"
     CDS             18038..19504
                     /locus_tag="VBE22_000804"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEO7202275.1"
                     /translation="MTNELIMQAFEWYLPSDGNHWKKLEESISDLKKLGISKIWLPPA
                     FKGTSSDDVGYGVYDLFDLGEFDQNGTIRTKYGRKEEYLKLIKSLKANGIKPFADIVL
                     NHKANGDHKEKFQVIKVNPENRQEALSEPYEIEGWTGFDFPGRQGEYNDFKWHWYHFT
                     GLDYDAKNNETDIFMIVGDNKGWADDDLIDDENGNFDYLMYNDIDFKHPEVIKNLQDW
                     AKWFIETTGIEGFRLDAVKHIDSYFIQTFINDIRTKIKPDLEVFGEYWKSDQTSMKDY
                     LEATQFQFSLVDVTLHMNFFDASHQNRDFDMRTIFDDSLVIDNPEYAVTFVENHDTQS
                     GQALESRVEDWFKPLAYGLILLRQQGTPCLFYGDYYGIQGEFGQPSFKEVIDKMAELR
                     QNYVFGEQVDYFTHSNCIGWTCLGDEEHNSCLAVVLTNGDQGWKHMEVGEIYAGKTFV
                     DYLGNCEQEVVIGDDGWGDFLVASASISAWVPKIEEAN"
     gene            19549..20547
                     /locus_tag="VBE22_000805"
     CDS             19549..20547
                     /locus_tag="VBE22_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7202276.1"
                     /translation="MKVLLYLEAEEYLKKSGIGRAIKHQEKALQIAGIDYTTNPTDDF
                     DLVHMNTYGIRSWLLMSKAKKTGKKVIMHGHSTEEDFRNSFIGSNLVSPLFKWYLCRF
                     YQKADAIITPTDYSKQLIKAYGIKKPIFVLSNGIDLSRYQRSEKKEAAFRHYFHLSKD
                     DKVVMGAGLYFMRKGIDQFVEVAAKMPDIRFIWFGETNKWVIPRKVRQIVTKQHPSNV
                     TFAGYIKGDVYEGAMSASDAFFFPSREETEGIVVLEALASHQHVVLRDIPVYHGWVTE
                     DSVELATDVDGFVEKLDKVLSGKSDKIKEGYRVAESRSIERIAHELASVYQKVMEL"
     gene            20549..21883
                     /locus_tag="VBE22_000806"
     CDS             20549..21883
                     /locus_tag="VBE22_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO7202277.1"
                     /translation="MRIGLFTDTYFPQVSGVSTSIRTLKEGLEKEGHEVYIFTTTDRN
                     VKRFEDPTIIRLPSVPFISFTDRRVVYRGLISAYRIAKDYELDIIHTQTEFSLGLLGK
                     LVAKALRIPVVHTYHTQYEDYVGYIAKGKLIKPSMVKYIMRTYLSDLDGVICPSRIVL
                     NLLDGYGVKIPKQVIPTGIPVENYRREDISEETIKNLRTELGLADNDTMLLSLSRVSF
                     EKNIQAILMHLSAIVDENLHVKLVIVGDGPYLSDLKELVHSLELENSVIFTGMVEHSQ
                     VAIYYKACDFFISASTSETQGLTYIESLASGRPIIAQSNPYLDDVISDKMFGTLYKKE
                     SDLADAILDAIAETPKMTQEAYEQKLYEISAENFSKSVYAFYLDFLISQKASVKEKVS
                     LTIGNKDSHSTLRFVRKAVYLPKKVFTFTGRASKKVVKAPKRRISSIRDFLD"
     gene            22340..24283
                     /gene="thrS"
                     /locus_tag="VBE22_000807"
     CDS             22340..24283
                     /gene="thrS"
                     /locus_tag="VBE22_000807"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEO7202278.1"
                     /translation="MIKITFPDGAIREFESGITTFEIAQSISNSLAKKALAGKFNGQL
                     IDTTRAIEEDGSIEIVTPDHEDALGVLRHSAAHLFAQAAKRLFPDLCLGVGPAIQDGF
                     YYDTDNKSGQISNDDLPRIEEEMKKIVKENHPCIREEISKEEALELFKDDPYKVELIS
                     EHAEDGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNAMMQRVYGT
                     AWFDKKDLKAYLKRREEAKERDHRKLGKELDLFMVNPEVGQGLPFWLPNGATIRRELE
                     RYIVDKEIASGYQHVYTPPMASVEFYKTSGHWDHYREDMFPTMDMGDGEEFVLRPMNC
                     PHHIEVYKHHVHSYRELPIRIAELGMMHRYEKSGALTGLQRVREMTLNDAHIFVTPEQ
                     IKDEFLKALNLIAEIYEDFNLTDYRFRLSYRDPEDKHKYYDNDEMWENAQAMLKEAMD
                     DFGLDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPIMIHRGGISTMERFTAILIETYKGAFPTWLAPQQVSVIPISNEAHIDYAWEVARV
                     LKDRGIRAEVDDRNEKMQYKIRAAQTQKIPYQLIVGDKEMEEKAVNVRRYGSKATETK
                     SIEEFVESILADIARKSRPDEVK"
     gene            24505..25209
                     /locus_tag="VBE22_000808"
     CDS             24505..25209
                     /locus_tag="VBE22_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7202279.1"
                     /translation="MRTVLVVQGDDETIELLRSYLEGALYKVVMASDGEEAFSLFQQH
                     QIDLAIIDITLPKIDGYELTRLIRQDSQIPIIMLAAKTTDMDRILGLNIGADDFITKP
                     FNSLEVLARINSQLRRYYEFNSLAKPKNQFIKIGELELDEEHVELTKNGKHIKLTATE
                     FKILHILMSSPGRIYTKTQLYEKINGRYLEGDETTIMVHISNIRDKIEDDSKYPKYIK
                     TLRGVGYKIEKPQYLV"
     gene            25307..26326
                     /locus_tag="VBE22_000809"
     CDS             25307..26326
                     /locus_tag="VBE22_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEO7202280.1"
                     /translation="MAKTALNIPFTVEGIENYYQNIKMILSLADKWSIPRDDLLLFLK
                     GLKAIDDIKVGSPIFKYFWTSLSLQAPLKALEFVLEQAKMPTELSGELSETQYLVAQF
                     SDELAPHDDFWIALSQVIYDSFPGNSLAEDTVLNRKLHQFRYLISSQQAQYVRRYFKD
                     VGMTDRDALVNYLSCLREPDSIAYYESARLHNKRRRNGEIFGFPDDEQVINSKLLISF
                     HTEFIIDDKGNFLNEIDAEVITRNGIINGASFNYAFKNNTRHKELDVDPVKLDPKFRN
                     DMTRGYRSPNLSRRKWFFFKEEDYDCSYFNKKGYYAFGRRSAKQSVDKQVKYLKKAVQ
                     KMRIN"
BASE COUNT         8495 a         4021 c         5431 g         8388 t
ORIGIN      
        1 actttatgag tggaatcaaa aagtttatga ttttctttgt catttggaaa ataaataaaa
       61 ccaaaaagtt ttaaactttt tggtttaact gctattaatt ggtctttaaa agacttaaaa
      121 attcttcaat tataggacta aaagaagtat catttcggta agcaatacca atcggataaa
      181 aattattttt atccttaagt gggacccagc ataaatcttg aacttggcta atgctttcaa
      241 attcacttgg taaaatagtg actgcattga ggtcttgtaa actatgaata agaacttccc
      301 agtcattgtg tttaaagacg atatgggggt caaatcctaa agcacgacat ttacgaggca
      361 acatctctcc tagcatgtaa tgttcgttga gtgaggcaac tttgtaatct cttaaatcat
      421 taagttcaat ttcaggaagt gttgcaagag gatgcgattt tggcatgaca atgcttactt
      481 tatatccttt agttgaggtt tgcagtggtt cgatagtgat atcattgcgg gtacttggaa
      541 aggacaataa gccaatatcg atctttcctt ttgctaacaa ttcttgtaat ttacgggaac
      601 catcttgaat caaagatact tcaacatttg aatgtgtagc cataaaagtt gaaatctgtt
      661 tcataaattg aatagcaaaa agagaagtaa gccctacacg gataccttcc tttttttctt
      721 gatttaattg ctggatttcc gtaaccattt gattaaaatt agtcatcaac aattgtcctt
      781 tctcatagag aatttctcct gcttcagtta aagcgagttg acgaccattt tgtaagaata
      841 aaatagtatt taattctgct tctaattttt taatggattg tgaaagtgta ggttgggtca
      901 caaaaagact ttttgcagcg tgtgaataat tttttgcttc tgcaacagca ataaagtaag
      961 taagttgtct aatatccata atatctgtcc ttttttctta gtaatagtat agcacaagtc
     1021 tatcaatctg acctttatga ttatttccac aataatatta gtaaaagcta tacttatata
     1081 attttttcca attttccttt tgcaaatagc tatgatatag ttaatatagt tagcgaaatc
     1141 acaaacaatc attattagta ggaggcatct tatgtcggat gttgtcgaaa aacaaacagc
     1201 taaatcgttt ataatgaacg tattaaatgg tttagcttta gggactgtta ttgtcttgat
     1261 accaggagca atccttggag aattaatgaa agctttactg ccaatgtgga gcggatttgc
     1321 aactttaatc gctgcaacag cagttgcaac aagtatgatg gggcttgtta ttggtattat
     1381 ggttggtctc aattttaaat ttaatcccat tcaatcagca tcactaggat tagcggttat
     1441 gtttgcaggt ggagctgcaa ccttccttaa aggcgctatt atgctaaaag gaacaggcga
     1501 tatcattaat atgggaatta ctgctgcctt aggtgtcctt ttgatccaat ttttatctga
     1561 caagacgaaa tcattcactt tgattgtcat tccaacagta acactcttac tagttggagg
     1621 agtaggtcat gtgttattac cttatgtcaa aatgattacc accatgattg gacaagggat
     1681 tgcttcatta cttggtttac aacctgtttt gatgtcaatt ttaattgcga tgattttctg
     1741 tttcttgatc gtatctccaa tcacaacagt tgggattgct ttggcaatta gtttgtccgg
     1801 aattggttct ggtgctgcta atttaggtat ctgtgctgct agttttgggc tttgtatggc
     1861 tgggtggtca gtcaattcta aggggacagc cttagcgcat gttttagggt ctcctaagat
     1921 ttcgatggct aatgtattag caaaaccgaa gattatgcta cctatgatat cttcagcagc
     1981 aatactagga attctcggag ctctctttaa tattcaagga acaccagcta gtgcaggttt
     2041 tggtatcagt ggtttgattg gaccgattaa tgctcttaat ttagcaaaag gtggatggtc
     2101 agttatgaat atgttattga taattataat atttgttgct gcaccaatta tattgaattt
     2161 tatttttaat tacctcttta taaaagtact taaaattatt gatccaatgg actataaatt
     2221 agatatttaa ggagatatta gtgatgaaac tcaaagtatt taatgttaga gaagaagaag
     2281 caacattagc acaagattgg gcaaaccgaa atcatgtgga actttctatg agtgaaggac
     2341 ctttgacatt ggaaactgtt aatgaggtag aaggttttga tgggattgct aatgcacaga
     2401 ttgggccttt agatgatgct atttatcctc ttctcaaaga aatgggaatt aagcaaatcg
     2461 ctcagcgcag tgctggtgtc gatatgtata atcttgagtt agctaagcaa catggtatta
     2521 tcattagcaa tgtgccaagt tactcaccgg agtctattgc agaatttaca gtgacaatcg
     2581 ctcttaacct tattcgaaaa gttgagctta ttagagctaa tgttcgtgaa cagaattttt
     2641 catggacctt gccaattcga ggacgagtcc taggtaacat gactgtggct ataataggaa
     2701 ctggtcgcat tggtctagcg actgctaaaa tttttaaagg gttcggatgt cgtgttattg
     2761 gttatgatat ttaccataat ccaatggcag atggtatcct tgagtatgtc aattcggtag
     2821 aagaagctgt tgaaaaagct gatcttgtgt ccttacatat gccaccaact gctgaaaata
     2881 cgcatctatt taatttagat atgtttaaac agtttaaaaa aggtgctatt ttaatgaata
     2941 tggcacgtgg agcattggtg gaaaccaaag atcttcttga ggctttagat caagggcttt
     3001 tggagggagc tggtattgat acttatgaat ttgaggggcc atatatacct aaaaattgtc
     3061 aaggtcagga catttcagac aaggatttcc taagactgat taaccatccg aaagtaatat
     3121 acactcctca tgcggcttat tatacagatg aagcggtcaa aaatctagta gagggtgcct
     3181 taaatgcttg tgtagaagtg gttgaaacgg gaacaactac tacgagagtc aactaaaaga
     3241 tgcttaggca tctttttttg tggactaaca aaacatatga cgattatgaa tcttaaagta
     3301 aaaaacatct tatgtattta tagcttaaaa gtctttttta taataaaaaa agatattttt
     3361 tataaaaata ttgacaaaac atcagatgaa tagtattatt taatcataaa agcgtttaca
     3421 aaaaacaagg agggttctct catggagaaa acaatggata tggaacaacg tgaagaagtt
     3481 gtatcttata accgagcgaa agtatggcag ttagttatgt ttgccatgaa caatgcttct
     3541 acaaatatat acttatttac gtttatgttt gttacctatt tctcaactgg cgttcttggc
     3601 ttggcagcca tttttgtcag tcaaattatg ggttatatcc gtatttttga tggttttatt
     3661 gatccagcta ttggtattat gattgataaa actgatacaa aatttggtaa gtatcgtcct
     3721 attttgatta ttgggaacgt tattacggct ttgtcattga tttttcttct tgcattgaga
     3781 ggtgtcgatg aaaatattcg tttcccatta tttatccttg ttctaattat ccataaaatt
     3841 ggatactcaa tgcaacagac aataactaaa gcagggtaaa cagcactgac aaatgaccct
     3901 aagcaacgtc ctatttttaa tatcgttgat gcagttatga caacatcatt aatgacaggc
     3961 ggtcagtttg tagtttcagg tttcttagta ccaaaatttg gtaattttac acctcaattc
     4021 ttcaatgtcc ttatttttgg aacaattctt atttcagcta ttttagcaat cgttgcaatt
     4081 attgggattt gggcaaaaga cagaaaagaa ttctttggtt taggagaaaa tacccaaaag
     4141 actgctctaa aagattactg gaaggtgttg aaaggaaata aaccgcttca gatattatcc
     4201 attgctgcag ctttggtaaa atttgccatc caattctttg gcgattccgt tgttatggtg
     4261 ctattgtttg gtattttatt tggtaattat gctttatcag gacaattctc acttcttttc
     4321 attgttcctg gtgttattat taatattttg ttctcaacaa ttgctcgtaa aaaaggtctt
     4381 cgtttttcat atgtcagagc aattcaggtc ggtatgattg gtttacttgc ttttggtgca
     4441 gttctttacg ttggtaagcc tggtgacctt agtttaacaa gcctaaatct ttatactatt
     4501 ttatttatcg ttacgaatat tattgcgaga tacgcttcac aagcacctgc tagtttagta
     4561 ttgactatgg gagcagatat ttctgactat gaaacttctg aatccggtcg ttatgtatca
     4621 ggaatgattg gaactatctt ctccttaaca gattcgattg cttcttcatt tgctcctatg
     4681 gtagtaggtt ttgtgttagc aggaattgga ttttcaaaat cgtttccaac tatcgaaaca
     4741 ccactgaccc ctgatttaaa aatggcagct attagtctcc ttgtggccat tccatttata
     4801 gctttgtcta tagcccttct tttaatgaaa ttttacaaac tagataaaga agaaatgttt
     4861 cgtattcaag agaagattca agttatgaaa gcagcaacta ctaaggaacg tgctaaagat
     4921 attgctcgta atgttccttt gacagatatg gattatgttg atgtaacaaa atatcctatt
     4981 gataaagatt aaaaagttta gctgctcttc aatagagcag ctattttgtt aaataagagc
     5041 taccattgga ggattcttat ggcaaaaatt atttcattag gtgaggttct tttgcgatta
     5101 tctccacctc aatatcacac actgatgcag gctaatcacc taaaatgcca atttggtggt
     5161 tcagaactta atgtcttagc aagccttgct caactaggtc accatgtagg attagtctca
     5221 gctttgcctg ataatgattt gggtaagatg gcaagacaat ttatattgtc acagcaaatt
     5281 agtccagctg ctataattaa aaaagaagga cgactaggaa tttattatta tgaacaagga
     5341 ttttcggtaa gaacaaataa ggttatctac gatcgtaact attcatcttt ttgggaaagt
     5401 accttgagtg attatgattt tacttctatt tttaagggag tggattggtt tcatgtttct
     5461 ggaattactc cagcactaac caaggactta tatgaggtga ctcggttcct tatgacaaaa
     5521 gctaaagaag gaggtgtaaa agtatcgatt gatctcaatt ttcgtgagag tttatggtca
     5581 tcttttcaag aggcgcgtga gcaattatcg ccactattag gattatcaga tgtttgcttt
     5641 ggcttagagc cgatttattt agctggcgaa agtgaagatt taaaagatga gttgggctta
     5701 tccagacctt accttgatat tgaattactt gaaaagataa ctcaaaagat tgttcaagaa
     5761 tatgggttag actatattgc ctttactcaa agggaaatgg gatatactaa tcagtatatg
     5821 ttaaaatcat atttgtatca taataatatg ttatatcaaa ctgataaaac tggcgttgaa
     5881 gtattagatc gtgtggggac gggagatgcc tttgctgcag gtttaattca tgcgcttctg
     5941 gaaaaagaaa ctcctcaaag ggccttagaa attgctatga ctacatttaa atacaaacat
     6001 accattcaag gagatatcaa tatcatgaca cgagatgata ttgcttacct cattgaaaaa
     6061 gaaacaaatg atattaaacg atagtgtaaa aggagagtgt atgttatatc cattattgac
     6121 aaaaacaaga aatacttatg atttaggcgg tatttggaac tttaaattag gagaacataa
     6181 tccaaatgaa ttactacctt cagatgaagt catggttatc ccgacttcat ttaatgattt
     6241 gatggtaagt aaagaaaaac gtgattatat aggtgatttt tggtatgaaa aagtcattga
     6301 agtacctaaa gtatcagagg gtgaagagat ggtgctacgt tttggctcag tgacacatca
     6361 agctaaaatt tatgttgacg gtatattggt aggggagcat aagggaggtt ttactccttt
     6421 tgaagttctt gttcctgaat gtaagtacaa taatgagaaa attaaggtat caatttgtgc
     6481 taataacgtt ttagactata ccacgcttcc ggtcggaaat tatagtgaaa tcattcaaga
     6541 agatggtagc attaagaaga aagtacgtga gaattttgat ttcttcaatt atgcaggtgt
     6601 gcatcgtcct cttaagttga tgatacgtcc taaaaatcat atttctgata ttacaattac
     6661 aagtcgtcta tcagatgatt tacagtcagc agatttacat tttttagttg agactaatca
     6721 aaaggttgac gaagttagaa ttagcgtttt tgatgaagat aacaaactag tgggtgaaac
     6781 gaaggatagc agattatttc ttagtgatgt ccatctttgg gaagttttaa atgcctatct
     6841 gtacacagcg cgtgttgaaa tttttgttga taatcaactc caagatgtct atgaggaaaa
     6901 ttttggtctt agagaaatag aagtgacaaa tggtcaattc ctattgaatc gcaaacctat
     6961 ttattttaaa ggatttggta aacatgaaga tacgttcatt aatggcagag gtttgaatga
     7021 agctgctaac ttaatggatt tgaatctttt gaaggatata ggggcaaact cttttagaac
     7081 atcccactat ccttattctg aagaaatgat gcgtttagca gatcgaatgg gagtgttagt
     7141 gatagatgag gttccagcag tagggttatt tcaaaacttt aacgcttcct tagatttgtc
     7201 accaaaagat aatggtacgt ggagtttgat gcaaacaaaa gctgcgcatg aacaagctat
     7261 ccaagaatta gtgaagcgtg ataaaaatca tcctagcgtc gtgatgtggg tagtcgctaa
     7321 cgaaccggct agtcatgaag cgggagcaca cgattatttt gagccattag taaaacttta
     7381 taaagattta gaccctcaaa aacgtcctgt caccttggtt aatattttaa tggcaacccc
     7441 agatagagac caagtgatgg acctggttga tgttgtctgc cttaatcgtt actatggttg
     7501 gtacgttgac cacggtgatt taacaaatgc agaagtaggt ctaagaaaag agttattaga
     7561 atggcaagat aaatttcctg acaaaccaat tatcataacg gagtatggcg ctgatacgtt
     7621 gccgggatta cactctactt ggaatattcc atatacagaa gaatttcaat gtgattttta
     7681 tgaaatgagt catcgtgttt ttgatggtat tcctaattta gttggtgagc aagtctggaa
     7741 ctttgcagac tttgaaacta atctgatgat acttcgtgta caggggaatc acaaaggtct
     7801 cttttcaagg aatcgccagc caaaacaagt cgtcaaagaa tttaaaaaac gctggatgac
     7861 tattcctcat taccataata aaaaaaatag tgtaaaatag gggtagttat tttagaagag
     7921 gtgatgatat ggctagaccc ttagtagaac aaacagcaga tcgtctattg catttaattt
     7981 tagaaagaga ataccctgtt ggtgctaagc tccccaatga atatgaatta gcggaagatc
     8041 tagatgttgg tcgcagcaca atccgagaag cagtcagaag tttagcaacg cgtaatattt
     8101 tagaagtcag acagggctct ggaacatata ttagttctaa aaagggtgtt tctgaggatc
     8161 cattagggtt ctctctaata aaggacacag ataggttgac atcagatctc tttgaactac
     8221 gccttttatt ggaacctcga atagcggagc tggtagctta tagaattaca gatgatcagt
     8281 tacaattact cgaaaagctt gttggagata ttgaagatgc tgttcacgca ggcgatccga
     8341 aacatttact tttagatgtg gaatttcatt ccatgctagc taaatacagt ggtaatattg
     8401 caatggatag ccttttgcca gtcatcaacc agtcaattca tctgataaat gcgaattata
     8461 caaatcgtca gatgaagtcg gatagtttag aagcacatag agaaattatc aaagcaatcc
     8521 gagaaaaaaa tccagtagct gcacatgatg ctatgcttat gcatatcatg agtgttagac
     8581 gttcagcatt gaaagcttaa tataagaaga aaaatagcta ttcttgaata gctatttttt
     8641 tagtattctt cttgacaatt catcagacga cttatataat atagataaag ataacggtta
     8701 cattttggga ggtattatgt taaatcaatt gaaaaacaac tatttcttcg ctgttattcg
     8761 aggaaaatca tctgaggatg cactagaaat cgcaaagcat gctattttag gtggtattcg
     8821 gaatattgaa gtaacttttt cgactcctga agcaagcaaa gtcatcaaac aattatcaga
     8881 tgactttaaa aataataaaa aaattattgt gggggccgga acggttatga caactgaact
     8941 tgctaaagat gctattgatg ctggagctaa atttttggtt agcccacact ttgattcaga
     9001 tattgctaat ttagctaatg aaaacaaggt ttattatttc ccaggctgtg caacggcaac
     9061 agaaatcgtg gtagctagaa agtataaatg tcagattata aagctttttc caggtggagt
     9121 tgtgggccca gatttgatca aagatattca tagtcctatt ccagatgttg atttaatgcc
     9181 atcaggtggc gtctctgttt caaatgtagc tgaatggaga aaagcaggag cagttgcggt
     9241 tggtgttggc tcagcattat ctagtaaagt agccacagaa ggctatgata gtgttactaa
     9301 aatagctaaa cagtttgttt ctgcacttga ttagtgagta ggagaagaat atgacgttta
     9361 atactgaaac atttatgtta aaaaatcaag cagctataca gctgtatgaa gaggtcaaga
     9421 ggcaacctat ttttgattat cattgccact tagatccaaa ggacattttt gaagatcgta
     9481 tttttgataa tattgtggat ttgtggcttg gtggtgacca ctataaatgg cgtcttatga
     9541 gggctaatgg tatttctgaa gcagaaatta caggtccagc atccaacctt gagaaattta
     9601 aatcatttgc tagaacacta gaaagagcat acggtaatcc tgtttaccat tggtctgcta
     9661 tggaattgaa aaatgtcttt ggtgttaatg agattttgac agaatcaaat gctgaggaaa
     9721 tttatcatcg tttaaatcat ttcttgaaag agcataaaat aagtcctaga cgtttaattg
     9781 cagatagcaa agttatgttt attggtacta cagaccatcc tcttgatact ctcaagtggc
     9841 ataaaaagtt ggctgctgat gagagtttta aaacagttgt tgctccgaca tttaggccgg
     9901 atgaagcttt tattgaacat cgacattttg ttgattttat aactaaactg ggtgacatta
     9961 ctcaaaaaga gattacagat ttttcaacat ttatagcagc aatggaagaa cgcattgcgt
    10021 attttgctca aaatggttgt agggctagcg atatcagttt tacggaaatc gtctttgaac
    10081 aaactgatga attggagttg aacgatttat ttaataaagt ttgtgaaggc tatatcccaa
    10141 atcaatcaga aatcagcaaa tggcaaacag ctgtttttat ggaactttgc cgattatata
    10201 aaaaatatgg ttttgtaaca caggtgcatt ttggagcttt gagaaataat cattctacta
    10261 tttttgaaaa attgggagct gacgtgggtg tggattcact tggtgaccaa gtggctttaa
    10321 ctgttaatat gaaccgttta ttggattcct tggttaaaaa agatagtctg cctaaaatga
    10381 tttggtataa tctcaatcca gcttacaata tagcagtagc taacactttg gcaaattttc
    10441 aagctaacga acttggagtg agatcttacc ttcaatttgg tgcaggttgg tggtttgcag
    10501 atactaaatt agggatgatt agtcaaatga atgccttagc agagcaaggc atgttggcga
    10561 attttattgg tatgttaaca gattctagaa gttttctatc ttatcaacgt catgactatt
    10621 ttcgacgtat tttatgtacc tacttaggag aatggattga agaaggagaa gttccagaag
    10681 attatcaagc tttaggaagt atggctaaag acattgctta tcaaaatgca gttaattatt
    10741 ttaagaacta aaaaggaaga gtgaaatcat ggaaatgtca tttaggtggt atggtgaaga
    10801 cgaccccgtt actttagaaa atattggtca aattccaaca atgaaaggga ttgttacagc
    10861 gatatacgat gtaccagtag gagaagtttg gtcacgtgag cgcattcaac agctgaaaga
    10921 aaaggtagag gcagcagggc ttaaaatttc ggttatcgaa tcagttcctg ttcatgaaga
    10981 tatcaaattg ggacgtccaa cacgtgacct cttgattgat aattatattc aaaccgtcaa
    11041 aaatttggct gcggaaggta ttgatactat ttgctataat tttatgccag tatttgactg
    11101 gacacgcact gatttagcat atcaatatcc agatggttct acagctttga tatttgatga
    11161 gacggtttct aaaaaaatgg atccagtaaa cggtgaatta tctttgccag gatgggatgc
    11221 cagctatagt aaagaagaaa tgaaggctat catggatgcc tatgctgaaa ttgatgagga
    11281 aaaactttgg gaaaatctca cttattttat taaacgtatt attcctgaag cggaagccgt
    11341 aggcgttaaa atggccattc atccggatga cccaccatat tctatttttg gtttacctcg
    11401 cattattact gggttagagg ctattgagcg ttttgtgaaa ttgtacgata gtaaaagtaa
    11461 tggcatcact ttatgtgtgg gttcttacgc ttctgatccc cagaatgatg ttttagagat
    11521 ttcgcgacgt gcttttgagt tagatcgtgt aaactttgtt catgctcgca atattaaatt
    11581 aggtgatgga aagtcattta aagaatcggc tcatccatca gaatacgggt caatagacat
    11641 gtatgaagtt attaagttat gccatgaatt tgggtttgag ggtgctattc gacctgacca
    11701 cgggcgtatg atttggggag aaacaggtcg tccaggatat ggcctatacg atagagcttt
    11761 aggagccact tacctttctg gtttatacga agctgttatt aaaggttcaa aataaggcga
    11821 tgcttccaaa tttctcctgt tccaatctcc tgcaagagaa atttggaaag ttattatcat
    11881 aatatgattg tacttagcta agagtagaat acttatagaa gttagtgaga gggataagat
    11941 gtcaaaaata attacattta ccaataaagt tgtcgtcata acaggtgcag gcggtgttct
    12001 ctgtggttat atggctaaag aatttgcgaa agcaggcgct aaagtggcat tattagattt
    12061 gaatcaagaa gctgctcaaa catttgctga tgaaattgta gcagaaggtg gtatcgccaa
    12121 ggcttataaa gctaacgttt tgtctaaaga aaaccttgaa gaagtgcatc aagccgtttt
    12181 agaggatctt ggaccaactg atattttagt taatggtgca ggaggcaaca atcctaaagc
    12241 cacaactgat aatgaatttc acgaattaga cttaccttca gaaacaaaaa ccttctttga
    12301 gttagatgaa gctggtatta gctttgtttt taacttaaat tatttaggaa cattattgcc
    12361 aacgcaagta tttgcccaag atatggttgg acgtgaagga gcgaatatta ttaatatctc
    12421 ttcaatgaat gctttcaccc ctctgaccaa aataccagct tattctggag ctaaagcagc
    12481 tattagtaac ttcacacaat ggctagctgt tcatttctca aaagttggta tccgttgtaa
    12541 tgctattgca cctggtttct tggtaacaaa tcagaaccga agcttgttat tcactgagga
    12601 cggtcagcca actgcgcgtg ctgagaaaat tcttaataat acaccaatgg gaaggtttgg
    12661 agaagcaagt gagctgattg gcggattgtt ctttttagca gatgaaaaat catcaagttt
    12721 tgttaatggc gttgtccttc cgattgatgg tggatttgct gcttactctg gtgtttaatt
    12781 tacaagataa tagccactta acatctaaga gattattgag ttgagtggtt ttttaaggaa
    12841 atttgtttaa tgatcttacc actaaatgat gggtaggttg ctaattggaa aatcctcata
    12901 agttagataa atttagaaac tggagataat atggtagata caaatcacaa aatcgctttt
    12961 tgcatagatt ccgatggttg cgcaatggat acaatgacat ataagcatca actctttttt
    13021 ggtccaattg ctgcagatat atttgggata tcagaacaag aaagaacaag ttttttaaaa
    13081 gagtgggata gagtcaatct ttattcaaga actcgtggag ttaatcggtt tgtgggattg
    13141 gttatgggcc ttgagtttgc cggagtagaa catattgata aattaaaaga atgggtcgaa
    13201 acaacagact cattgtctaa taattcctta gagttagcgc ttgccaacga ttttagtaga
    13261 gatttagaga aggctttaga atggtccaat gaagtgaatc accaaatcaa aaattatgag
    13321 ggagattctt tagcgttttc aggtgtgtta gaaagtttag aacgattaag tcaatttggt
    13381 aatgtttatg tcgtgagttc tgctaacaag gaagctgttt ggcaagagtg ggaagaacag
    13441 ggattgctaa agtatgttga tgatatctat tgtcaagata gaggtaaaaa agaagatgtt
    13501 atcgccactt taattgcaag tggttactgc cgtgaaacta ttttgatggt aggagattcc
    13561 ccaggtgatt tggctgcagc agaacaaaat ggtattgcat tttacccagt acttgttggt
    13621 aaggaagtca aatcatggga aattcttaga gaagatattg gtgaagcttt tgcaaaaggt
    13681 caattcgaac agcaaaggca aaaagaatct attaatacct tttgggctaa tttagataac
    13741 taatagaaag gatgggctat gacacattta gttgacttaa caaagaaacc cttcaatcta
    13801 aatcaagaag caattgagtg gattgaaaaa acaattaatg agatgacatt ggatgagaaa
    13861 attgggcaac tttttttcaa tatgggagct agtcgttcag aagaatattt aacagatgtt
    13921 ttggatcggt atcatattgc tgctgttcgc tataatagag gtagttcaag tgaaatttat
    13981 gatcagaatt taattttaca gacaaagtcg aaattaccga tgttgattgc tgcaaataca
    14041 gaagctggtg gagacggtgc tgtcacagat ggaactaaag ttggcgatga aataaaagtg
    14101 gctgctacaa atgatccgaa atatgcttat gaaatgggaa gaatcgcagg gatggaggca
    14161 tctgctgtgg gttgtaatgc ttcatttgct ccaattgtag atttgacacg taattggcgt
    14221 aatcctatca tagctagtcg aaattggggt gctaacgttg atcaaattat ctcactttca
    14281 aaagagtata tgaaaggaat catgcaatat aatattgtac ctttcgctaa acattttcca
    14341 ggggatggca ttgatgagcg tgatcatcat ttgtcatttg cttccaatcc tatgagtaaa
    14401 gaggaatgga tgtcgacatt tggccgcatt tatggtgaat tggctgacgc aggtctacca
    14461 ggagtcatgg caggtcacat acatttacca aatgttgaaa aagaaatgca tccagagcga
    14521 gaccttgatg atatgttacc agcttcattg aataaaacac ttttagatga gttattgaga
    14581 ggagagttag gttataatgg agctattgta acggacgctt ctcatatggt tggaatgact
    14641 gcttcaatgc caaggagaga tttgttgcca acggcgattg aagcaggttg tgacttattc
    14701 ttattcttta atgaccccga tgaagacatt caatggatga aagaaggtta cgaaaaaggt
    14761 attctaacag aggaacgtct ccatgacgct ttacgtcgta ctctcggtct aaaagcgaaa
    14821 ttaggcttgc ataactatga gggacgtcgc caagaactct ttatgcctaa agataaggct
    14881 atggctttaa tcaacacatt agagtcgcaa aaaatagctg atgaagttgc tgataaagca
    14941 gtaactctgg taaaagataa acaaaaagat atctttccag taaatccaga acgttatcgt
    15001 catatcttac ttgttaacgt tgaaggatat aagggaggtt ttggtgcgat gattgctgga
    15061 aacaaacaac gtgcatcgga ttacatgaaa gagttacttg aggcacgtgg ccatgaagta
    15121 actgtctggg agtctaccga agagcgcatt atgaaattgc ctcaagagga gcgggcagca
    15181 gctatcgcta atgtttacgc acaaaaacag ccaatcgcaa atctaacaga acactatgac
    15241 ttgattatta acttggtaga tgtcaatgct ggaggaacaa cacaacgtat tatttggcca
    15301 gcagctaaag gcacccctga tcagcctttt tacgttcatg aaataccatc tattgtgatt
    15361 tcagtacaac atgcatttgc tttagcagat atgccacagg tagggactta tattaatgct
    15421 tatgatgggc ttcctagcac gataagtgca gtagttgcga aattggctgg agaatcagaa
    15481 tttacaggag tttcaccagt agatgcttac tgtggtttaa ttgatacaca tttatggcgc
    15541 caatgctaat agaaacaaga tgatgtactt ttatgtaaca taaaaaaaga gcttaaagct
    15601 ctttttttat ccttcaaaat agagtagttc cttgggtgtt ttggtaaata cttcaaagcc
    15661 ggttttagtc acataaccgc agtcttcaat tcgcacacca actttatctg gtatataaat
    15721 acctggttca acagagaagc acatgccttc ttgaatctcc atatcgttac cagccataat
    15781 agatggaaat tcatggacat ccatacctaa tccatgacca aggcggtggt taaagtattg
    15841 tccataacca gccttttcaa taacatttct tgctgctgcg tcaacttcag aagcaaggac
    15901 tcctggctta ataaaatcaa tagccgcttg atgtgcttca agacataaat ggtaaatatc
    15961 ttttttgaat tgatcaggtt tccccactgc tactgtacgg gtcatatctg acgtataacc
    16021 tagtgtttct acacctaaat caaataataa caaagcatta ttttcgattt tattagttcc
    16081 tgggatacca tgtggatttg ctgcattatt gccagtcaaa accatagtgt cgaaactcat
    16141 tttattgata ccttgttttt tcatttcaaa ttcgatttga gcgataatat cggtttcagt
    16201 gttgtttagc gagatattgt caaatccaac ttgaacagct ttatcagcaa attctccagc
    16261 gactaacatt ttctcaattt catctcgtga tttaattagg cgcatgtttt gaatataagg
    16321 tgttaggttt tcaaaatgac catcaaaaac ggtttggaga ccttggaatt tagtaacatt
    16381 aagattatca aattcggcta gaactttaga aacagaaaag gatggtaagt tgctagctat
    16441 tttttgccaa ggattttctg aatctatgta accaaagacg ggaaatggta cactttgttt
    16501 tgcacgcgat acttctagtg ctggtacaaa taaaatagga tcgcgatctt catagacaaa
    16561 taaaaacatt tgtctttcat ggggatcgca aaagaaacct gttaaatagt tgactgtcac
    16621 aggatcagaa aagacagcaa gttcagcttg gactgaatgt aagtgatggc gtatacgatt
    16681 aagttttgac ataataaccc ttcttcctaa aaggaactta gtctagttag gctagtttct
    16741 tatgcctatt atttttccaa aaaaatagga aaaaatcaag tttagttagt attttgaaag
    16801 tttttcataa aaaagcttta taaaagcttg aaagtgtttc caaaaggtga taaaatatca
    16861 gtgaaagcgt cattttcaaa tagaaaggaa gttaaatcaa tatgaataca gatgatacga
    16921 ttacgattta tgatgttgcc cgtgaagcag gtgtttcgat ggcaactgtt agtcgtgtgg
    16981 taaatggaaa taaaaatgtc aaggaaaata cacgtaagaa agtccttgaa gttattgatc
    17041 gtcttgacta tcgtccaaat gctgtggcgc gtggtttagc aagtaaaaaa acgacaactg
    17101 ttggtgttgt cattcctaat atcgcaaata gctacttttc aattttagcg cgtggtattg
    17161 atgatattgc agcaatgtac aaatataaca ttgtccttgc ttcaagtgat gaagacgatg
    17221 ataaagaggt taatgttgtt aatactttgt ttgctaagca agtagacggt attattttta
    17281 tgggacacca cttaactgaa aagattcgtg cagagttctc tcgttcacgc acaccgattg
    17341 ttttagcagg tactgtggac cttgagcatc aattaccaag tgttaatatc gattacaaag
    17401 cagcagcagt tgatgttatt gatattttag ctggcaatca taaagacatt gcttttgtat
    17461 caggaccact tattgacgat attaatggca aggttcgttt ggcaggatac aaagaaggtc
    17521 tcaagaaaaa tggcttaaac tttaaagagg ggttggtttt tgaagccaac taccgttatg
    17581 ctgaaggctt tgcattagct caacgtgtga ttaatgctgg agcaacagca gcatatgttg
    17641 ctgaggatga gttagcggca ggtttgttaa atggtctttt tgaggcaggt aaacgtgttc
    17701 ctgaagattt tgaaattatc acaagcaatg actcgccaat cgcacaatat acacgtccta
    17761 acttaacttc tatcagtcaa ccggtctatg atttgggtgc tgttagcatg cgtatgctaa
    17821 caaaaattat gcacaaagaa gagttggaag aaaaagaaat agtgcttaat cacgggattg
    17881 ttaaacgtgg cacaacaaat aattagtgtt agagtatgtt atttggaaaa tagtggtaga
    17941 atggtagtag cttagcgctt aactttctga tacttaacat gatatattga gaggattagg
    18001 gtagcttagc ttcctaatct ttttttagag gtgtaatatg acaaatgaat tgataatgca
    18061 agcttttgag tggtatttac ctagtgatgg gaatcactgg aagaaattag aggagtctat
    18121 atcagacctt aaaaaacttg gaattagtaa aatctggtta ccaccagcat ttaagggaac
    18181 tagcagtgat gatgtaggat atggtgttta tgatctcttt gatttaggag aatttgacca
    18241 gaatggaaca attagaacaa aatatggtag gaaagaagag tatctaaagc ttattaagtc
    18301 gttaaaggca aatggcatta aaccgtttgc agatatcgtt cttaaccata aagccaatgg
    18361 tgatcataaa gaaaaatttc aagtcatcaa agtcaatcct gaaaatcgtc aagaagcatt
    18421 aagtgaaccc tatgagattg aaggatggac gggatttgat ttcccaggta gacagggtga
    18481 gtacaatgat tttaaatggc actggtacca ctttactggt ttagattacg atgctaaaaa
    18541 taatgaaaca gacatcttta tgattgtagg cgataataaa ggttgggcag acgatgattt
    18601 gattgatgat gaaaatggta atttcgatta tctcatgtac aatgacattg attttaagca
    18661 tcctgaagtt ataaaaaact tacaagattg ggctaagtgg tttattgaaa ctacaggcat
    18721 tgaaggtttt cgtttggatg ctgtgaaaca tatcgattct tactttattc aaactttcat
    18781 taatgatatt cgtacaaaaa taaaacccga tttagaggtc tttggcgaat attggaaatc
    18841 tgaccaaaca tcaatgaagg actatttaga ggcaacacaa tttcaatttt ctcttgttga
    18901 tgtcaccttg catatgaatt tttttgatgc cagtcaccaa aaccgtgatt ttgatatgcg
    18961 aacaattttt gatgatagtc ttgttattga taatcctgag tatgctgtta cattcgtaga
    19021 aaatcacgat acgcaaagtg gacaagctct agaaagtaga gtggaagatt ggtttaaacc
    19081 tttagcttat ggcttgatac ttttacgcca acaagggact ccatgtcttt tttatggtga
    19141 ttactatggc attcaaggag aatttggcca accatccttt aaagaggtta ttgataaaat
    19201 ggctgagcta cgccaaaact atgtatttgg cgaacaagtc gattacttta cgcattcaaa
    19261 ttgtattggt tggacttgtc ttggggatga ggaacataat tcttgtttag ctgtggtcct
    19321 aacaaatggt gatcagggat ggaaacatat ggaagtaggg gaaatttatg caggcaaaac
    19381 ttttgttgat tatttaggca attgtgaaca agaggttgtt ataggagatg atggttgggg
    19441 tgatttttta gttgcatctg cgtctatctc ggcttgggtc cctaaaatag aggaagctaa
    19501 ctgataaatc aggtgaaaaa aaaggtaaaa tgagatataa taagacctat gaaagtttta
    19561 ctgtatttag aggctgaaga atacttgaaa aagtcaggta tcggtcgtgc tatcaaacat
    19621 caagagaaag ctcttcaaat tgctgggata gattatacca ctaatcctac agatgacttt
    19681 gaccttgttc atatgaatac ctatggtatt agaagttggc ttttgatgag taaagctaaa
    19741 aaaactggga aaaaagtaat tatgcatggc cattccactg aagaggactt tagaaattct
    19801 tttattggct caaatttagt atccccgctt tttaaatggt acctttgccg tttttatcaa
    19861 aaagcagatg ccattataac cccaactgat tattctaaac aactaataaa agcatatggc
    19921 atcaagaaac caatttttgt tttatctaac gggattgatc taagtcgcta tcagaggtca
    19981 gaaaagaaag aagctgcatt tcgtcactat tttcacttaa gtaaggatga taaagttgtt
    20041 atgggggccg gtctttattt catgcgtaag ggaattgacc aatttgttga agtagctgca
    20101 aaaatgcctg atattcgttt tatttggttt ggagaaacaa ataaatgggt tattcctcga
    20161 aaggttaggc aaatcgttac caagcaacat ccctcaaatg ttacttttgc tggttacata
    20221 aaaggtgatg tttatgaagg tgccatgagt gctagcgatg ctttcttctt tccgagtcgt
    20281 gaagagacag aagggatagt agttttagag gctttagcta gtcatcagca tgtggtttta
    20341 agagatatcc ctgtgtatca cggttgggtg accgaagaca gtgttgaatt agcgactgat
    20401 gttgatggtt ttgttgaaaa gttggataaa gtcttatctg gtaagagtga taagattaaa
    20461 gaaggttatc gtgtggctga gagtcgaagt atcgaacgaa ttgcccatga attggcaagt
    20521 gtttatcaaa aagtaatgga gttgtaagat gcgtataggt ctatttacag atacatactt
    20581 tccccaagtt tcgggagttt caacaagtat ccgaacttta aaggaagggt tggagaagga
    20641 aggacacgaa gtttatattt tcacaactac cgaccgtaat gtcaaacgtt ttgaagaccc
    20701 tacaattatt cgtttaccaa gtgtcccttt tatttctttt acagatcgtc gagtagttta
    20761 tagaggttta atttctgctt ataggatagc taaagactac gaacttgata ttattcatac
    20821 tcaaacagaa ttcagtttag gacttttagg aaaattagtt gcaaaagctt taagaatacc
    20881 tgttgtgcat acttatcata ctcaatatga ggattacgtt ggttatatcg caaaaggcaa
    20941 attgattaag ccaagtatgg tcaaatatat catgcgtacc tacctaagtg atttggatgg
    21001 tgtcatatgt ccttctcgaa ttgttctcaa tcttctagat ggttacggtg tcaaaattcc
    21061 aaaacaagtt attccgacag gtattcctgt tgagaactat cgtcgagagg atatctctga
    21121 agaaacaatc aagaatctac gaacggagct tggacttgcg gataatgata caatgctatt
    21181 gagtttatca cgggtatctt ttgaaaaaaa tattcaagca atcttgatgc atttatcagc
    21241 tatcgttgat gaaaatcttc acgtaaaatt agtgatagtt ggagatggtc cttatctttc
    21301 agatctaaaa gaacttgttc actctctaga acttgaaaat tcagttattt ttacaggtat
    21361 ggttgagcat agccaagtgg ctatctacta taaagcatgt gatttcttta ttagtgcttc
    21421 aacaagtgaa acgcaaggat taacttacat tgagagtctg gcgagtggac gtccaatcat
    21481 tgcgcaaagt aatccttatc tagatgatgt tatttcagat aaaatgtttg gaactctgta
    21541 caagaaggaa agtgatttgg cagatgctat cctagatgca atagcagaga cacccaaaat
    21601 gactcaagaa gcttatgaac agaaattata tgagatttct gctgagaatt tcagcaagtc
    21661 tgtatatgct ttttacctag actttctgat ttcacaaaaa gctagtgtga aagaaaaagt
    21721 ctctctgaca ataggaaata aggatagcca ttctactctt cgttttgtca gaaaagcggt
    21781 atatttacct aagaaagttt ttacctttac aggaagagcc tctaaaaaag tggtaaaagc
    21841 acctaaacgt cgtatttcat ctattcgaga ttttttagat tgagacgaat aaaattagta
    21901 aatgtcttga aattataaaa aatattgtta taataaaaaa tgaagacatg ttaggttcta
    21961 atagatttta agagcgagca ctggttggtg gaaagagtgc aattagagaa cttagtacta
    22021 cttccttgat tttatgcaaa cataaaaggg tggttccctt atcgccacta gaggtctgac
    22081 acaagtatac tcgagttttt gtgttggata aattgaggtg gaaccacgta gtctttacgt
    22141 ccttttggat gtaaaggctc tttttttata aaagaagtgg ttgcataaaa gaggcagatt
    22201 gctttgctaa tgttatactt attatcccta gctaggataa tgtggaattc aatcaaaggt
    22261 caatcccttt gtttattagt gaattaatgt gtttaggaat tggtatcacc ctaacagtta
    22321 ttaagaaaag gagaacaaga tgattaaaat cactttccca gatggtgcaa ttcgtgaatt
    22381 tgaatctggc atcacaacat ttgaaattgc acaatcaatt tctaactctt tagctaaaaa
    22441 agctttagct ggtaaattta atggtcaact tattgataca acacgtgcta tcgaagaaga
    22501 tggtagtatt gagattgtaa cacctgacca cgaagatgct ttaggagttc ttcgtcactc
    22561 agcagcgcat ttatttgctc aagctgcaaa acgccttttt ccagacttat gtttaggtgt
    22621 aggtccagct attcaagatg gtttttacta tgatactgat aataaatcag gtcaaatttc
    22681 aaatgatgat cttcctcgta ttgaagaaga aatgaaaaaa attgttaagg aaaatcaccc
    22741 ttgtattcgt gaagaaatca gtaaagaaga agcgttagaa ctttttaaag atgaccctta
    22801 taaagttgaa ttaattagtg aacatgctga agatggttta actgtttatc gtcaaggtga
    22861 atttgtagac ctttgtcgtg gacctcatgt cccatcaaca ggacgtattc aagtctttca
    22921 tcttctaaat gtcgctgggg cttactggcg tggtaacagt gataatgcta tgatgcagcg
    22981 tgtctatggt actgcttggt ttgataaaaa agatttgaaa gcatatctta aacgccgtga
    23041 agaagctaag gaacgtgacc accgcaaatt aggtaaagaa cttgatttat ttatggttaa
    23101 tcctgaagta ggtcaaggat tgccattctg gttgccaaat ggagcaacta ttcgccgcga
    23161 attggaacgt tacattgttg ataaggagat tgcttcaggt tatcaacacg tttacacacc
    23221 tccgatggca tctgttgaat tttacaagac ttctgggcac tgggaccact atcgtgaaga
    23281 catgtttcca acaatggata tgggagatgg agaggaattc gttcttcgtc caatgaactg
    23341 cccacaccat attgaagttt ataaacatca tgttcactca taccgtgaat tgccaattcg
    23401 tatcgctgaa cttggtatga tgcaccgtta tgaaaaatca ggggccctca caggattgca
    23461 acgtgtacgc gaaatgacac tcaacgatgc ccatattttc gttacacctg agcaaattaa
    23521 agatgaattt cttaaagctc ttaatctcat agcagaaatt tatgaagatt ttaacttaac
    23581 agattaccgt ttccgcttat cttatcgtga cccagaagat aaacataagt attatgataa
    23641 cgatgagatg tgggaaaatg cacaagctat gctaaaagag gctatggatg attttggatt
    23701 agattatttt gaagcagaag gcgaagcagc cttttacggt cctaaattgg atatccaggt
    23761 gaaaactgct cttggtaatg aagaaacatt atctactatc caactagatt tccttcttcc
    23821 agaacgcttt gatcttaaat acatcggtgc agatggtgaa gaacatcgtc caattatgat
    23881 tcaccgcggt ggtatttcaa caatggagcg ctttacagct attttaattg aaacttacaa
    23941 aggtgccttt ccaacttggc ttgctccaca acaagtttct gttattccaa tttcaaatga
    24001 agcccacatt gattatgctt gggaagtggc tcgtgtcctt aaagatcgtg gtattcgtgc
    24061 tgaagtggat gaccgtaacg aaaagatgca atataagatt cgtgcggctc aaacccaaaa
    24121 aattccatat caattgattg ttggtgataa ggaaatggaa gaaaaagccg taaatgtgcg
    24181 tcgctatgga agcaaagcga ctgaaacaaa atctatagag gaatttgtag aaagtatttt
    24241 agctgatatt gcaagaaaat ctagacctga cgaagttaaa taaattttat tagtcattat
    24301 aaaaatcatt aataaatgat gtgaactgat tactgtatga tgcagaatca gttttttaga
    24361 tgctaatttt ttcttgatgt ttaattataa gctaattata gataaataat ctcatataac
    24421 attcacaaaa ttagattttt ttggtataat taatatgatt gacacataaa ggaaaatagt
    24481 attaataaaa aggggtatgg agcattgcga acagtattag tagtgcaagg tgatgacgaa
    24541 acaatagagt tattaaggtc gtatctagag ggagctcttt ataaggttgt gatggctagt
    24601 gatggtgagg aggctttttc tttatttcag caacatcaga ttgatttagc aatcatagat
    24661 attacgctac ctaaaataga tggatatgag ttgacgcgac tcattcgtca agatagtcag
    24721 attcctatta ttatgctagc agctaagaca acagatatgg acagaatatt aggattgaat
    24781 attggagcag atgattttat cacgaaaccg tttaattctt tggaggtttt agctcgaatt
    24841 aattcgcaac tgcgtcgtta ctatgaattc aatagtcttg ccaaacctaa aaatcaattt
    24901 ataaagatag gggaattgga attagatgag gaacatgttg aactaactaa aaatggtaaa
    24961 catatcaagt tgactgctac agaatttaag attcttcata tcttaatgtc atcaccagga
    25021 aggatttata ctaaaacaca actctatgag aagattaatg gtaggtattt agaaggtgat
    25081 gagacaacga ttatggtgca tatttctaat atccgtgaca aaatcgaaga tgatagtaaa
    25141 tatcctaaat atataaaaac acttagagga gtaggttata aaatcgaaaa accgcagtat
    25201 cttgtttaaa aacataatgg ctagtaagag ccatttttta gtgaagatat ttcttggttt
    25261 taagctttcc tttttgctat aataagtaaa attggagggg agagatatgg caaagaccgc
    25321 tcttaatatt ccttttacag ttgaagggat agaaaattat tatcaaaata taaaaatgat
    25381 actgtcttta gcagataaat ggagcatacc tagagatgat ctattacttt ttttaaaggg
    25441 actcaaggct attgatgata taaaagttgg ctctcctatt tttaagtatt tttggacttc
    25501 tttaagttta caagcacccc tcaaagcttt agaattcgtg ttagagcagg ctaaaatgcc
    25561 aactgaattg tcaggagaat tatcagaaac acaatacttg gtcgcacagt tttcagatga
    25621 gttagctccg catgatgatt tctggatagc cttatcacaa gttatttatg attcttttcc
    25681 tgggaatagt ctagccgaag atactgtatt aaataggaaa ctccatcaat tccgctattt
    25741 gatttctagt cagcaagcac agtatgttcg acgatacttt aaagatgttg gtatgactga
    25801 tagagatgct ttagtaaact atctatcatg tttaagagaa ccagatagta tcgcctacta
    25861 tgaatctgct cgtttgcata ataaacgtcg gagaaatggt gaaatctttg gtttcccaga
    25921 tgatgagcag gttattaata gtaagctttt aatttcattc catacggaat ttattattga
    25981 cgataaagga aactttttaa acgagattga cgcagaagta ataacacgta atggcattat
    26041 caatggagct agctttaatt atgcttttaa aaataataca cgtcataaag aattagatgt
    26101 tgatcctgta aagttagacc caaaatttcg aaacgatatg actagaggtt atagatcgcc
    26161 aaatctatct agaaggaaat ggtttttctt taaagaagag gattacgatt gtagttattt
    26221 caataaaaaa ggatattacg catttggaag gcgtagtgct aagcaatcgg ttgataaaca
    26281 agtcaaatac ttgaagaagg ctgtgcagaa aatgcgcatc aactgataca tatac
//
LOCUS       DATRTN010000018        24482 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.018, whole genome shotgun sequence.
ACCESSION   DATRTN010000018 DATRTN010000000
VERSION     DATRTN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24482
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.018"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            137..1675
                     /locus_tag="VBE22_000810"
     CDS             137..1675
                     /locus_tag="VBE22_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7202281.1"
                     /translation="MNKSFNTKLGLVTVAALSGIVLTSQLPVNAKAETPVMAASAQQG
                     FRFVATVVDSQTRVLPGKLVTLSEVTSGQPKIIASVKTNDAGQAIFNNLPIKRNLSVS
                     VDGQVKGYTIRTDVAGSSKAASFTATGVGTNEPTYSKKTIDITVRDQNAEPVSGRTVT
                     LKTQAGREIASLVSGDNGLTRFTDRLLDGTFYQYFVDGKKIGDIVPGESRSAYVNVAP
                     KKDYFTFTVTALDKDGLVVKGKEVTLTDITDGKAVALASLKTNDNGQAIFTNLPLSRN
                     ISVSIDGKSKGYTIRTDVAGSHKAAAFYVDGKGTKAPQFSAEAAVVTVYDANGNTIAN
                     QEVTLTNSNGTIVAKGLTEKDGKARFANKLMAGTLYNIFVNGIEMPKTALVGSDVSVF
                     LTDKQIKKEKTVTPDKLDKEMNQEKEKELLDTPKPIPPTSKPTAPIKNKKDDSPKKFT
                     KSQSSSLKSSVKAIGAKPKAMTALAKKLPKTGDQAISILTLVGFIVTGLAIMLGFLKK
                     SRSK"
     gene            1852..3396
                     /locus_tag="VBE22_000811"
     CDS             1852..3396
                     /locus_tag="VBE22_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEO7202282.1"
                     /translation="MTLQDEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLEELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VIDPLAKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPELFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWINSDDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     KLEEKM"
     gene            3482..3862
                     /locus_tag="VBE22_000812"
     CDS             3482..3862
                     /locus_tag="VBE22_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEO7202283.1"
                     /translation="MGIFSGLMGNASQMDTDKVENQLSDILISDEQVDLAYTLIRDLI
                     VFTNYRLILVDKQGVTGKKVSYNSIPYASISRFTVETSGHFDLDAELKIWISSAIEPA
                     EVLQFKNDRNIVSIQKALAIAVLK"
     gene            4056..4790
                     /locus_tag="VBE22_000813"
     CDS             4056..4790
                     /locus_tag="VBE22_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7202284.1"
                     /translation="MIIELKSVGKIFPKSQFRLDDISFNIEEGEIVGLIGSNGTGKST
                     ILKMINGLIPYDKGNIYYQGKEVKSFSDNKLRQMRKDIAYIFQNHNLLVGESVYYHLA
                     LVYKLNHQKVNHDAINDILDFLGLMDLKQVKCHSLSGGQQQKVAIAMAVLQKPKLILC
                     DEISSALDTNSEKEIFNLLSDLREKYGISILMIAHHLSLLKQYCDRVMILDHQTIVDT
                     VVPVKATLNQLESNYVDQVKEFLLHD"
     gene            4783..5445
                     /locus_tag="VBE22_000814"
     CDS             4783..5445
                     /locus_tag="VBE22_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7202285.1"
                     /translation="MIEIIEKHGEGILKSFWETNLMLGLTLILCFLIAFPTGILLFSL
                     RKSYLIKHSLAYQLLNLFLGTLRSVPFLIFIFILIPLNRLIFGTSFGTIAAILPLTLV
                     SVSLYARYVEQALLNIPQVVVDRALSLGANKRQIIYYFLIPSIKIDLVLSFTATAISI
                     LGYSTIMGVIGAGGLGEYAYRFGYQEYDYPVMYLIVVLFIIYVFILQSLGYFIANRYS
                     RK"
     gene            5461..6291
                     /locus_tag="VBE22_000815"
     CDS             5461..6291
                     /locus_tag="VBE22_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202286.1"
                     /translation="MKKLLSLTCLIMMSLCLVACTKQAMSSKQIKDKNSKEKVITVAT
                     YSKPTSTFLDLVKDNVKEKGYTLKVVMVSDYIQANIALENKEHDANLLQHEFFMSIFN
                     KENDGHLVSITPIYHSLAGFYGQHLKNIAELKDGAKVAIPSDPANMTRALLLLQEKKL
                     ITLKNTSKKTKAIEDILTNPKKLRIEPVALLNLNQAYFEYDLVFNFPGYVTKINLVPK
                     RDRLLYEKKPDIRFAGALVAREDNKNSDKIKVLKEVLTSKEIRHYITKEIPSEADVAF
                     "
     gene            6526..8112
                     /locus_tag="VBE22_000816"
     CDS             6526..8112
                     /locus_tag="VBE22_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7202287.1"
                     /translation="MKFTELNLSQDILSAVEKAGFVEPSPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIHTEDNTIQALIIAPTRELAVQSQEELFRFGRDKGVKVRS
                     VYGGSSIEKQIKALRSGAHVVVGTPGRLLDLIKRKALKLNHIETLILDEADEMLNMGF
                     LEDIEAIISRVPEARQTLLFSATMPDPIKRIGVKFMKNPEHVKIKATELTNVNVDQYY
                     VRVKENEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDHIDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLTIIENLTKKRMTGMKPATASEAFQAKKKVALKRIARDFEDQ
                     ELVSKFDKFKADALELATQYTPEELALYVLSLTVQDPESLPEVEITREKPLPFKPSGG
                     GFKGKGGRGNGRGGDRRRNDRGDRRGNRDRDDRGGRRDFKRRDDKFKKDNRCQENKKP
                     HKNTSSEKQTGFVIRNKGDK"
     gene            complement(8297..8563)
                     /locus_tag="VBE22_000817"
     CDS             complement(8297..8563)
                     /locus_tag="VBE22_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO7202288.1"
                     /translation="MIKVPAYMYVLECSDGTLYTGYTTDVKRRLNTHNTGKGAKYTRA
                     RLPVKLLYSEAFNSKQEAMRAEALFKQKTRQAKLTYIKQHKNEQ"
     gene            complement(8556..9320)
                     /locus_tag="VBE22_000818"
     CDS             complement(8556..9320)
                     /locus_tag="VBE22_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEO7202289.1"
                     /translation="MIDTILKENERIDQLFSTDVKIIQNKEVFSYSIDSVLLSRFPKL
                     PSRGLIVDLCSGNGAVGLFASTKTNATIIEIELQERLADMAKRSIKLNKLEKQVTMIN
                     DDLKNLLDHVQRSNVDLMLCNPPYFKASETSKKNLSPHYLLARHEITTNLREICQIAQ
                     HALKTKGRIAMVHRPDRFLEIIDTMRQFNLAPKRIQFVYPKLGKDANMLLIEAIKDGS
                     TEGMKILPPLVVHQDNGDYTETIFDIYFGENGKSHD"
     gene            9455..10195
                     /locus_tag="VBE22_000819"
     CDS             9455..10195
                     /locus_tag="VBE22_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEO7202290.1"
                     /translation="MFYTYLRTLVMFLIWVANGNAHYHNEDKMLKDDENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFTNRLFGWWIKMCGAFPIDREKPGQDAIRYPVKM
                     LKNSNRSLVMFPSGSRHSKDVKGGVAVIAKMAKVRIMPAAYRGPMVFKNLLKGHRVDM
                     NFGNPIDVSDIKRMDAEGIAEVSRRIQEEFDRLDRENETYDDGKKLNPLTYIYRLPLA
                     IIAIVLLVLTLIFSYLASFVWDPQKHLK"
     gene            10293..10946
                     /locus_tag="VBE22_000820"
     CDS             10293..10946
                     /locus_tag="VBE22_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEO7202291.1"
                     /translation="MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDNLN
                     INLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGAVNHPGVYSLPSQSRVTDAIKRA
                     GGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
                     ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTID"
     gene            10930..13167
                     /locus_tag="VBE22_000821"
     CDS             10930..13167
                     /locus_tag="VBE22_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEO7202292.1"
                     /translation="MLQLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFTFIFL
                     IYQYDRETIFKTIAIVIFFLFYFLWQNHNMNVQYQRVPNHISQIKVRIDTISINGDVL
                     SFQADASGNTYQAFYTLKNKSEKDYFQNLDNNIMIIADIKLEEAEERRHFNGFDYRQY
                     LKRHGIYRIAKVTKIKQIRLFQHRSFFDLMFKWRRSAIVISQTFPNPMRHYMSGLLFG
                     YLDKTFDDMSDLYSSLGIIHLFALSGMQVGFFLGIFRYICLRIGLRLDHVWLLQIPFS
                     LIYAGLTGFSISVVRALIQSLLSHSGVKKDENFALCLLICLISLPHSLLTTGGVLSFA
                     YAFILTMTSFDHFSSIKKVAIESLTVSVGILPILTYYFSGFQPISIILTALLSFAFDI
                     IFLPLLTVIFVLSPIVKLGCINSLFEILEVLLKWTGQLFPRPLIFGKPSLFLLIVMII
                     ILGLLYDYYHSKCFRYCSLLIIFTLFFITKNPITNEVAILDVGQGDSILVRDWLGKTI
                     LIDTGGRVRFEQPEEWKQKVNQSNAERTLIPYLKSRGISKIDDLVITHTDTDHMGDME
                     VISKHFKVARLITSSGSLTNSQYVKRLSKIGVAVKSIEAGDKLAVMGSYLQVLYPWHK
                     GDGKNNDSIVLYGHLLGKGFLFTGDLEEEGEKQLLEAYPNLSVDILKAGHHGSKGSSS
                     LSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKSKIFRTDQSGTIRLTGWWKWH
                     IQTVR"
     gene            13293..14102
                     /locus_tag="VBE22_000822"
     CDS             13293..14102
                     /locus_tag="VBE22_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7202293.1"
                     /translation="MTIKRIFCDMDGTLLNSEGQVSKSNATLIREAAIPVTLVSARAP
                     MEMKDAVDALQLGGVQVAFNGGLIYRIGDNNQVLPIHTQIIKKSTVKQLLRGIRFHFP
                     QVSLSYYDLNNWYCDKIDEGIRYEHSLTQQCPTFIHNEDQFLEGHTNTFKIMMITFDE
                     ANMLELEKYLQSLELPEITIQRSGKAYLEITHLLAKKSKGIAYILQKEQLAREETAAF
                     GDGHNDLPMLEMVGYPIVMDNAFDDIKAIAYQLTKSNDEDGVGYGIQKFLK"
     gene            14113..15057
                     /locus_tag="VBE22_000823"
     CDS             14113..15057
                     /locus_tag="VBE22_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7202294.1"
                     /translation="MTSISDIAKKAGVAKSTVSRVINHHPHVSDETRQKVMALITELD
                     YIPNQLARDLSRGKTQKIGVVIPHTRHPYFTQLINGLLDAAKTTDYQLVMMPSDYNKE
                     LELSYLKQLKMEAIDALIFTSRAISLDIIETYAKYGRIVVCEKLQEYNHLSSAYLDRY
                     SSFLEAFSDMKLRGLEHLVLLFSRNNESSATYQSALLAYQEVYGQLSSPYMVVGNVHD
                     FNEGLNLSYQLVKEVSIDGILATSDEVAAGLIKGYEESRKKRPYIIGQECLLVGQLLK
                     LPTIDHKSYYLGKLAFKQALAEKISQEVLFSEFLSQDN"
     gene            complement(15116..16108)
                     /locus_tag="VBE22_000824"
     CDS             complement(15116..16108)
                     /locus_tag="VBE22_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7202295.1"
                     /translation="MKNYRKLIVLLLLIFFVIFTGAYAYTHIVEKRSLTSNTIEKTLP
                     VVNQIKPQTIKEYQNYLTKVAKRNVLPVDIPQALNNEKVEITATDGMQTFTWNDKNNP
                     KQKVIFYVHGGSYIHQASELQYIFVNKLAKKLDAKVVFPIYPKAPTYNYSDAIPKIKK
                     LYQNTLASVTSHKQIILVGESAGGGLALGLADNLVTEHIKQPKEIILISPWLDIATNN
                     PKIEKVQKKDPLLKAWQLQQVAPYWANGKKNFKNPQVSPLYSSQFNKMAPISFFIGTH
                     DIFYPDNQLLHQKLAKENIKHHYIVGQKMNHVYPVLPIPEAETAFKQMLKIINH"
     gene            16284..17012
                     /locus_tag="VBE22_000825"
     CDS             16284..17012
                     /locus_tag="VBE22_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7202296.1"
                     /translation="MFHREDFSKALLKKALQPGMRVMDIGCGSGELTRLAADIVGKEG
                     DVVGIDINEQLLWQARHDNGYANVSYQNADLAAFSSFETKSFDAIIGRRVLMYQKNPK
                     KVIESLTPLLKQNGFYVFQESDAMCLELAKPSFVNMNKIQSLIWQTVEKEGGHIHIGS
                     QLEQLMLEAGLIVDHFVSENVIQTSQTGTDLSWLLNIMKERIQKYSDLQGLEDVNLQK
                     ELEENNDHFIRDCQFGIISHRVDE"
     gene            17066..18103
                     /locus_tag="VBE22_000826"
     CDS             17066..18103
                     /locus_tag="VBE22_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEO7202297.1"
                     /translation="MIAIEEIGRITPDNLGLVTVLAGEDLGQYAQMKEKLFQVIGFNK
                     DDLAYSYFDLSEEDYQNAELDLESLPFLSDYKVVIFDQFQDITTDKKTYLDEQAMKRF
                     EAYLQNPVDTTRLVICAPGKLDGKRRLVKLLKRDARVLEANTLKESDLKTYFQKYAHQ
                     EGLVFEAGVFDELLIKSNYDFSDTLTNIAFLKSYKTDGHISSNDVREAIPKSLQDNIF
                     DLTQDVLLGRIDLARDLVRDLRLQGEDEIKLIAIMLGQFRMFLQVKILASKGKSESQI
                     VSELSHYIGRKINPYQVKFAVRDSRNLPLAFLKEAIRILIETDYAIKRGTYDKDYLFD
                     LALLKIAHKKP"
     gene            18183..18791
                     /locus_tag="VBE22_000827"
     CDS             18183..18791
                     /locus_tag="VBE22_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEO7202298.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALG
                     KHPEIGEDLEALLADVSQIPEDIRQAVINNGGGHLNHALFWELMSPEETQISQELSED
                     IDATFGSFEDFKAAFTAAATGRFGSGWAWLVVNAEGKLEVLSTANQDTPIMEGKKPIL
                     GLDVWEHAYYLNYRNVRPNYIKAFFEIINWNKVNELYQAAKA"
     gene            19129..19980
                     /locus_tag="VBE22_000828"
     CDS             19129..19980
                     /locus_tag="VBE22_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO7202299.1"
                     /translation="MIIKRVLNHNAVISVTHQGLDVLLMGKGIAFKKRIGDRINSDAI
                     EKSFVLKNSDNMNRFTELFITVPEEVVACSERIINLGKIKLGKNLDEILYINLTDHIH
                     SAIERHEQGMVIQNPLRLEIQRYYPDEYSIGMKALELIKDELGICLTIDESAFIAMHF
                     VNAGLDNPFNEAHKITEIVSYIEQKVKTDFRTELDESSIDYYRFMTHTKLFAQRVLSG
                     MKYEDDDADLLLVVKKKYPREYKCVKEIGNNMAIQYQYQLNSSELLYLTVHVKRLVKN
                     LKEISDD"
     gene            19973..21841
                     /locus_tag="VBE22_000829"
     CDS             19973..21841
                     /locus_tag="VBE22_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEO7202300.1"
                     /translation="MTKYQETAKAIFAAVGGEKNIQHVTHCVTRLRLVLDNDEIVNDQ
                     VIKTIPNVIGVMRKNDQYQIILGNDVNNYYNAFLTLGHFENTTREFSSQKKSSILEKL
                     IETIAGVITPLIPALLGGGMLKVIGILLLMLGIASSSSQTVAFINFFGDAAYYFMPIM
                     IAYSAASRFKVTPVLAATVGGILLHPAFVTMVAEGKPLSLFGAPVTLASYGSSVIPIL
                     IMVFLMQYIERWINKIVPSIMKSFLQPTLIILISGFLALVVVGPLGVIIGKGLSSAML
                     SIYHVAPWSALAILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQG
                     AASFAVAVKAKQKQTRQVAFAAGLSALLAGITEPALYGVTLKFKKPLYAAMISGGLVG
                     AYIGLVNIASYTFVVPSIIGLPQYINPQGGNNFSNAVIAAIATIILTFIITWFLGIDE
                     GENEKSSINAQEHTHIRSGLSKKETLYSPMVGNVLPLSKVPDETFSSKLLGEGLAITP
                     SVGEVYAPFDSEIISLFPTKHAIALKDDKGVEVLIHIGIDTVELNGEGFEQLVKVGDF
                     VKRGQLLLRMDIDFISSKGYSLISPVVVTNSIDQLEIIVKDEETMVTNEDDLLVIL"
     gene            21858..23285
                     /locus_tag="VBE22_000830"
     CDS             21858..23285
                     /locus_tag="VBE22_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO7202301.1"
                     /translation="MTVFPKHFLWGGAVAANQVEGAFRTDGKGLSVQDVLPNGGLGDF
                     TAKPTPDNLKLEAIDFYHNYKNDIKLFAEMGFKVFRTSIAWSRIFPNGDDSAPNEAGL
                     QFYDNLFDELLKYNIEPLVTLSHYETPLHLAKTYNGWADRRLIAFFEKFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGAIMTDKSQLSPQELYQAIHHELVASARVTKLGRSI
                     NPNFKIGCMILAMPAYPMTSDPRDVLAARQFEQHNLLFSDIHVRGKYPTYIQSYFKNN
                     GIKIKFEEGDEEVLAQNTVDFLSFSYYMSVTQAYDFENYQSGQGNILGGLTNPHLTTS
                     EWGWQIDPIGLRLVLNQYYERYQIPLFIVENGLGAKDQLIETLDGDYTVEDDYRIDYM
                     NQHLVQVAKAIEDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGTGSLQRY
                     KKKSFGWYQKVIRTNGQSLFEHHNR"
     gene            complement(23354..24448)
                     /locus_tag="VBE22_000831"
     CDS             complement(23354..24448)
                     /locus_tag="VBE22_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7202302.1"
                     /translation="MKLRKQLAQQIVTSIKDVCQQDINFINTKGIIFASTNPKRVGEF
                     HEIGLKVAQTGQMIEVTDQESYFGTQAGINIPFYYNCELLATIGISGNPNQVGKYALL
                     AQKMTRLILKEHELDYLDFGRKNEASIVLHHLVEGRELDYYYLNQFLNQYHLSEKTDY
                     RLLTFEINSQKQKLLLSQSEMSLLNFFDKLDTAIYTFNYPNQYWLLLSDQMFDYYYPN
                     ILSKFECKKGLYKVGIGQKSSLSLLKRSYETSILALKALKGQQKVNLVDDLDLELLLT
                     SIDSNIKQYVLNKALVNLSENDKILLNSYFKHNLSLKECSQELFIHKNTVQYRLNKIY
                     ESTQLNPRNFKDATLLYLALKSEDINLHKL"
BASE COUNT         8036 a         3637 c         4710 g         8099 t
ORIGIN      
        1 tagctggata atgtttttca tttttagcta tttttatgat tgaccgctaa ttattaatct
       61 gctaagatat agtacgtaat aaaaacttaa ccggttaatt atttttgaaa tatatttttt
      121 ctggaggaaa atagtaatga ataaatcatt caataccaaa ttaggtttgg taactgtagc
      181 ggcattatca ggtattgtat taacgtctca attacctgtg aacgctaaag ctgagacacc
      241 agtaatggca gcaagtgcac aacaaggttt ccgttttgta gcaaccgttg tagatagtca
      301 gacgcgtgtc ttgccgggaa aattggtaac tttgtcagag gtgacaagtg gtcagcctaa
      361 aattattgca agtgtgaaaa cgaatgatgc cggacaagct atttttaata atctccctat
      421 aaaacgtaac cttagtgtgt ctgttgatgg acaagtaaaa ggatatacta ttcgaactga
      481 cgtagcaggc tctagtaaag cagctagttt tacagcaaca ggagtaggga caaatgagcc
      541 aacgtattca aaaaaaacca tagacattac agtgagagac caaaatgctg aacctgtttc
      601 aggcaggaca gttaccttaa aaacacaagc tggtcgtgag attgctagct tggtttctgg
      661 tgacaatgga ttaactcgct ttactgatcg tttactagat ggaacattct accaatattt
      721 tgttgatggg aaaaaaattg gtgatattgt acctggagaa tcaagaagtg catatgttaa
      781 tgttgcacct aaaaaagatt attttacctt tacagtcact gctttagata aagatggact
      841 tgttgttaaa ggaaaagaag taacgttgac tgatattact gatggtaaag cagtagcgct
      901 tgcttcttta aaaacgaatg ataatggaca agctatcttt accaacctac cattatcaag
      961 aaatatcagt gtttcaattg atggaaaatc aaagggctat actattcgaa ctgatgtagc
     1021 aggctctcat aaagcggctg cattctatgt agatggtaaa ggtaccaaag cacctcaatt
     1081 tagtgcagaa gcagcggttg taactgttta cgatgctaat ggaaatacta tagcaaacca
     1141 agaagtgaca ttaactaatt caaatgggac aattgttgca aaaggtttga ctgaaaaaga
     1201 tggcaaagca aggttcgcca ataaattaat ggcaggaacg ttatacaata tttttgtaaa
     1261 tgggattgag atgcctaaaa ctgcccttgt tggtagtgat gtcagtgttt ttctaacaga
     1321 taaacaaatt aagaaagaaa aaactgttac tcctgataaa ttggataaag aaatgaacca
     1381 agagaaggaa aaggaactct tagatactcc aaaaccaata ccacctactt cgaaaccaac
     1441 agcaccaatt aaaaataaaa aggacgattc ccctaagaag ttcacgaaaa gtcaatcttc
     1501 tagtttgaag tcaagtgtta aggctatagg tgctaagcca aaagctatga cagccttagc
     1561 taaaaaattg cctaaaactg gtgatcaagc cattagtatt ttaactttgg ttggttttat
     1621 tgtaacaggg ttagcgatta tgttaggctt ccttaaaaaa tcacgatcaa aataatttat
     1681 tttagaagct tgggtcttta gacacaggct tctcttcttt cttcggaaat agattgaact
     1741 tgaagaatgt tgttaaattc gctataataa taaagttata gacaagatta ttgtttcagt
     1801 tgagactact gaaaataata ctaagaaatg gataaaagct aaggattgaa tatgacatta
     1861 caagacgaaa tcaaaaaacg ccgtactttt gccatcatct ctcacccgga tgctggtaag
     1921 acgactatta ctgagcaact attatatttt ggtggtgaaa ttagagaagc agggacagta
     1981 aaagggaaaa aatcaggtac ttttgcaaag tccgattgga tggatattga aaagcaacgt
     2041 ggtatctctg ttacttcatc tgttatgcaa tttgattacg cgggtaaacg tgttaatatt
     2101 ttggatacac ctggacatga ggatttctcg gaagatacat accggacctt aatggctgtg
     2161 gatgcagctg tcatggttgt agattcggct aaaggtattg aggctcaaac taaaaaactt
     2221 tttgaagttg ttaagcatcg taatattccg gtttttacgt ttattaataa attggaccgt
     2281 gatggacgtg agccactgga tttgcttgaa gagttagaag aagtactagg tattgcttct
     2341 tatccaatga attggccaat tggtatgggg aaatcatttg aagggcttta tgatttacat
     2401 aataaacgtt tagaactcta taagggagac gagcgttttg caagtattga agacggtgat
     2461 cagctgtttg ccaataatcc attttatgaa caagtcaaag aagatattga acttcttcaa
     2521 gaagcaggta atgacttttc agaacaagcg attcttgatg gtgacctaac tccagtattc
     2581 tttggctctg cccttacgaa ctttggagtt cagacttttc ttgatacttt cttggagttt
     2641 gctccagaac ctcatggtca taagactaca gaaggtaatg ttattgaccc cttagctaaa
     2701 gatttttcag gttttgtctt taaaattcaa gctaacatgg acccacgtca tcgcgaccgc
     2761 attgcttttg ttcgtattgt ttctggtgaa tttgagcgtg gtatgggagt taatttgaca
     2821 cgcactggca aaggcgctaa gctctccaat gttactcaat ttatggcaga atcacgagaa
     2881 aatgtgacga atgctgttgc tggggatatt atcggggttt atgatacagg aacttatcaa
     2941 gttggggata ccttaacggt tgggaaaaat aaatttgaat ttgaaccatt accaactttt
     3001 accccagaat tatttatgaa agtatcagca aaaaatgtta tgaagcagaa atcattccac
     3061 aagggtattg agcaattagt acaagaagga gcaattcagc tttataagaa ttatcagaca
     3121 ggcgaataca tgctgggtgc tgtgggtcaa cttcagtttg aagtttttaa acatcgtatg
     3181 gaaggtgaat acaatgctga agttgttatg acaccaatgg gtaaaaagac tgtacgttgg
     3241 attaactcag acgatttaga cgaacgcatg tcttcaagcc gaaatatttt ggcaaaagat
     3301 cgttttgacc aaccagtttt cctatttgaa aatgattttg cacttcgctg gtttgctgat
     3361 aagtatcctg atgtgaagct tgaagaaaaa atgtaaaatt taaagtcact caaatttgag
     3421 tgacttttta agttttttag actaaaatag ttataataca tagaaaagat tggagattaa
     3481 catgggaatt ttttcgggat taatgggaaa tgcttcacaa atggataccg ataaagtcga
     3541 gaatcagtta tctgatattc tgatttcaga tgaacaggtt gatttagctt ataccttaat
     3601 tcgtgatttg attgttttta ctaattatcg tttaatttta gttgataaac agggggtgac
     3661 aggtaaaaag gtttcttata attctatacc ttatgcttct atatcacgtt ttacagtgga
     3721 aactagtggt cattttgatt tagatgctga gttaaagatt tggatatcat cagcaataga
     3781 accagcagaa gtacttcagt ttaagaacga ccgtaacata gtgagtatac agaaagcttt
     3841 ggcgatagct gttttaaaat aaacaattta gcaagtgttg ctactaagag ttatttgttt
     3901 taattataac ttttaatagt gctaaattct cattttcaaa ataaaagtag tgatttttat
     3961 cactgctttt attttgaagt atagtaggcc tcaagcatat attttgaaaa cgctgtattt
     4021 ttttgataag atattttttt gaggagtatt aaataatgat aatagaatta aagagtgttg
     4081 gaaaaatttt tccaaaatca cagtttagat tagatgatat ttcttttaat atagaagaag
     4141 gcgagatagt tggtctgatt ggttctaatg gtactggaaa gagtactatt ttgaagatga
     4201 ttaatggctt aatcccttat gataaaggaa atatttatta tcaaggaaaa gaagtgaagt
     4261 ctttttctga taataagtta cgtcagatgc gtaaagatat tgcttatatt tttcaaaatc
     4321 ataatttgtt agtaggagaa agtgtatatt atcatctagc tttagtttat aaattaaatc
     4381 atcaaaaagt aaatcatgac gcaattaatg atatcttaga ttttttaggt ttgatggatt
     4441 taaaacaggt gaaatgccat agtttgagtg gtgggcagca acaaaaagtt gcaattgcta
     4501 tggctgtttt gcaaaaaccg aaacttattc tatgtgatga gatcagctct gccttagata
     4561 ctaatagtga aaaagagata tttaacttgt tgtctgatct tcgtgaaaaa tatggcatct
     4621 ctatcttgat gattgctcat cacctatctc ttttaaaaca atattgtgat cgtgtaatga
     4681 ttttggatca ccagactatt gtagatactg ttgttccagt gaaagcaact ctgaatcaat
     4741 tagagtcaaa ctacgtagat caagtaaagg agtttttgtt acatgattga gattattgaa
     4801 aaacatggcg aaggtatctt aaaatcattt tgggaaacca atttaatgtt agggttaacc
     4861 ttgattcttt gttttctaat tgcttttcca acaggtattt tattgttttc attgagaaag
     4921 tcttacttga ttaaacattc tttagcctat caactattaa atttattttt aggtacgtta
     4981 cggagtgtcc ctttccttat ttttattttt atcttaatcc ctctcaatcg attaattttt
     5041 gggacctcat ttggaacgat agcggcaatc ttacctctta ctttggtttc agtcagtttg
     5101 tatgcacgtt atgtggaaca agctttatta aatatacctc aagttgttgt tgatcgagct
     5161 ctcagtctag gggctaacaa gagacagata atttattatt ttttaatccc ctccatcaag
     5221 attgaccttg ttttatcatt tactgcaacg gctataagta ttcttggcta ctcgacaata
     5281 atgggagtta ttggtgcagg aggtttgggt gaatatgcct atcgttttgg ctatcaagaa
     5341 tatgactacc cagttatgta cttgattgtt gtcttattta taatatatgt gtttatctta
     5401 cagagtttgg gctattttat agcaaaccgc tactcaagaa aataaaagaa aagaggaact
     5461 atgaaaaaat tactttccct aacatgtcta atcatgatgt ctttatgttt agtggcatgt
     5521 actaagcaag caatgtcgtc taagcaaatt aaagataaga atagtaaaga aaaagtgatt
     5581 actgttgcaa cttacagcaa acctacatct acctttttag atttggttaa agataatgta
     5641 aaagaaaaag gatatacttt aaaggttgtc atggtctctg actatattca ggctaacatt
     5701 gctttagaaa acaaagaaca tgatgctaac cttttacaac atgaattttt catgagtatc
     5761 tttaataagg aaaatgatgg tcatctagtg tcaattacac caatttatca ttcattggct
     5821 ggtttttatg gtcaacattt gaaaaatatt gccgagctta aagacggtgc taaggtagcg
     5881 attccgtctg atcctgccaa tatgactaga gctctgcttt tattgcaaga aaagaaactt
     5941 atcaccttaa agaatacgtc caaaaagacc aaggctatcg aagatattct tactaaccct
     6001 aaaaaattac gaattgaacc tgtagcatta cttaacctca atcaggccta ttttgaatat
     6061 gaccttgtct ttaatttccc tggatatgtg acaaaaatca atctagttcc taaaagggat
     6121 agattattat atgagaaaaa accagatatc cgttttgcag gtgccttggt agctcgtgaa
     6181 gataataaaa atagtgataa aataaaagta cttaaagaag tactaacaag taaagagatt
     6241 cgtcactata tcactaagga gattccaagt gaagcagacg ttgcgttcta gatagataaa
     6301 ctatgaaccc tccttcttta tagatggagg gtttttattg ccttttttct attttctgaa
     6361 gcaactaata gaactagcaa tttatgaaag cgtgttattt attagattga ccttatttta
     6421 tatcttttta cttttaattg tgttacacta agtaggttgt cagaaaagat aactaataaa
     6481 ggttcataag gcagcttcgc actgtctttt agaaaagaga aacatttgaa atttacagaa
     6541 cttaacttat cgcaagatat tctttcagcg gtagaaaaag caggctttgt agagccatca
     6601 ccaatccaag aaatgactat cccacttgct cttgaaggca aagatgtcat tggtcaagcg
     6661 cagacaggta caggaaaaac tgcagctttt gggttaccaa ctttaaataa aattcatact
     6721 gaagataata ctattcaagc acttattatt gctcctacac gtgaacttgc agtacaatca
     6781 caagaagaat tgttccgttt tggacgcgat aaaggtgtta aggtccgctc tgtttatggt
     6841 gggtcaagta ttgaaaaaca aatcaaggca ttacgttcag gagcacacgt tgttgttgga
     6901 actccaggtc gattacttga tttaattaaa cgtaaagcac ttaaacttaa tcacattgaa
     6961 actcttattc ttgatgaagc agacgaaatg cttaacatgg gatttttaga ggacattgaa
     7021 gcaatcatta gtcgtgtacc agaagcacgt caaactcttt tattctcagc gacaatgcct
     7081 gatccaatca aacgtatcgg ggttaaattc atgaaaaatc ctgagcatgt taaaattaaa
     7141 gcaactgaat taactaatgt gaatgttgat cagtattatg ttcgcgttaa ggaaaatgaa
     7201 aaattcgata ctatgacacg tttgatggac gttgatcaac cagaattgtc aattgtcttt
     7261 ggacgtacga aacgtcgtgt agatgaatta actcgtggac taaaattacg tggtttccga
     7321 gcagaaggaa ttcatggaga cttagatcaa aataaacgtc ttcgtgttat cagagacttt
     7381 aaaaatgacc atattgatat tcttgttgcc acagatgttg ctgcgcgtgg tttagatatt
     7441 tcaggcgtta cacatgttta caactatgat attccccaag atccagaaag ttatgttcac
     7501 cgtattggac gtactggtcg tgctggtaaa tctggtcaat ctatcacttt tgtttcacca
     7561 aacgaaatgg gatatttgac aattattgaa aacttaacta aaaaacgtat gactggaatg
     7621 aagcctgcta ctgcaagcga agcatttcag gctaagaaaa aagttgcatt gaaacgtatt
     7681 gcacgtgact ttgaggatca agaattagtt tctaaatttg ataaatttaa agccgatgct
     7741 ttggaattag caactcaata cacaccagaa gaacttgctc tttatgtctt aagcttaaca
     7801 gttcaggatc cagaatcgtt accagaagtt gaaatcacac gtgaaaaacc attaccattt
     7861 aaaccatcag gtggtggctt taaaggtaaa ggtggacgag gaaatggccg tggtggcgat
     7921 cgtcgacgca atgatcgtgg tgatcgtcgt ggaaatcgcg accgtgatga tcgtggtggt
     7981 cgtcgtgatt ttaaacgtcg tgatgataag ttcaagaaag ataatcgttg tcaagaaaat
     8041 aaaaaacctc ataaaaacac ttcaagtgaa aaacaaactg gatttgttat tcgcaataaa
     8101 ggcgataaat aagaggatta cgaaaaagga gttgctgagc gactcctttt ttattttttg
     8161 taagataaag cgtagatagt gatatccata aaattgcctt ataactttaa gaatgaagac
     8221 aaaaggttaa agtgaggttg aaatttaaaa gttaaagaag cgaggatagg ctcattacac
     8281 ttagtatatt atcgtattat tgttcatttt tatgttgttt aatataagtc aattttgctt
     8341 gccgagtctt ttgtttaaat aatgcttctg ccctcattgc ttcttgtttg ctattaaaag
     8401 cttcagaata aagaagtttt actggcaatc tcgcacgagt atatttagca ccttttccag
     8461 tattgtgtgt attgagacgg cgcttgacat ctgtagtata acctgtataa agtgttccat
     8521 ctgaacattc taaaacatac atataagcag gaactttaat catgagattt tccgttttcc
     8581 ccgaaatata tatcaaaaat agtctcagta tagtctccat tatcttgatg aacaacaaga
     8641 ggaggaagta ttttcatacc ttctgtcgag ccatctttaa tagcttcaat taaaagcata
     8701 ttagcatctt ttcctaattt tggataaaca aattggatac gtttaggtgc taaattgaac
     8761 tgacgcatcg tatcaataat ttcaaggaaa cggtctggac gatgtaccat agcgatacgt
     8821 ccttttgttt ttagagcatg ttgagcaatt tgacaaattt ctctaaggtt tgttgttatt
     8881 tcgtgtcttg caaggagata atgcgggctt agattttttt tagaggtttc gcttgctttg
     8941 aaataaggtg gattgcaaag cattaaatca acgtttgaac gttgaacatg atcaagaaga
     9001 ttttttaaat catcattaat catagtaacc tgtttttcca atttattaag cttaatcgaa
     9061 cgcttagcca tatccgcaag gcgctcttgt agttcgatct ctataattgt cgcatttgtt
     9121 ttggtgctgg caaacaaacc gacagcacca ttaccactgc ataagtcaac gataagacca
     9181 cgagaaggaa gtttagggaa ccgagacaat aaaacactat ctatggagta ggaaaaaact
     9241 tctttgtttt ggatgatttt gacatcagtt gaaaaaagct gatcaatgcg ttcattttct
     9301 tttaaaattg tatctatcat aggcttatta tagcaaaatt acagcttcct agcactagtt
     9361 ttgaaagtgt ggaggttgtt tttaagataa ttcttcctaa aaaaaaggaa gcatgataaa
     9421 atagaagtta cttgatgaaa ggtagaggag aaacatgttc tatacttact tacgtacttt
     9481 agttatgttc ttgatttggg tggctaatgg taatgcgcac tatcataatg aagataaaat
     9541 gttaaaagat gatgaaaatt atattttagt agctccacac agaacatttt gggatcctgt
     9601 gtacatggct tttgctgcta gacccaaaca gtttattttt atggccaaaa aagaattatt
     9661 cacgaatcgt ttgtttggtt ggtggattaa aatgtgcggt gctttcccta ttgatcgtga
     9721 aaagccaggg caagatgcta ttcgatatcc tgtaaagatg ctgaaaaata gtaaccgttc
     9781 tttggtgatg tttccgagtg gaagccgcca ctccaaagat gtcaaaggag gcgtagctgt
     9841 tattgcaaaa atggcaaaag tcagaattat gccagcagca tatagaggtc ctatggtttt
     9901 taaaaatcta ttaaaggggc atcgtgttga tatgaatttt ggtaacccta ttgatgtttc
     9961 tgatattaag cgtatggatg ccgaagggat tgcagaagta tctcgtcgta ttcaggaaga
    10021 atttgatcgt ttagatagag aaaatgaaac ttatgatgat ggtaagaaat taaatccatt
    10081 gacttatatt tatcgtctac ctttagcaat tatagctatt gtcttgcttg tcctaactct
    10141 aatttttagt tatttagcaa gctttgtgtg ggatccacaa aaacatctaa aataagtgtt
    10201 acatggaaac tatttacttt ttagtaaata gttttttgtt tttaatagga aaatctcttt
    10261 agttacgaat aagtaaatgg aggaatattt ttatgtttga aatagtattg gaaaaaatta
    10321 aatcgcataa atgggagaca acaggaatta ttgtaggact cttattattt ggaattttgg
    10381 gtttaaatca ttttggcact catcataaag aagacaattt aaatatcaat ttagagaaaa
    10441 aagtatcaac tataacagaa aaaaaggtac caatgattag tcacgtcaaa gataaagtat
    10501 ctaatcaagt aacggttgac gtaaaagggg cagtcaatca tccaggtgtt tatagtttgc
    10561 cttctcaaag tcgtgtaaca gatgcgatta agcgtgcagg tggactaagc aatctcgctg
    10621 atagtaaatc cgttaatcta gcacagaaat tacaagatga gacggttatc tatgttgctc
    10681 aaaaaggtga aaaaataact gttgtggaag aggagaaagc aaataatatc gccactcagg
    10741 gaaatagtaa ggggaaaatt aatcttaata aagcagattt atcaagtctt cagaccatat
    10801 caggcgttgg agctaaaaga gctcaagata ttttagatta tcgtgatagt caaggtggtt
    10861 ttaaaactat tgatgacctc aaaaacgtat caggtattgg tgagaaaaca ctagaaaaat
    10921 tacgtcaaga tgttacaatt gactaagtat tttcctctaa aacctattta tttagcattg
    10981 ttggtcttcc aaatttactt actagtgttt tcttggacaa tgcttggttg tgcctttctt
    11041 ttatttactt ttatttttct gatttatcaa tatgatcgtg aaactatttt taaaacaata
    11101 gcaatagtaa tttttttctt attttatttt ttatggcaaa atcacaatat gaatgtccaa
    11161 tatcaaagag taccgaatca tattagccag attaaagtgc gtattgatac tatttctatc
    11221 aatggtgatg ttttatcatt ccaggcagat gcttcaggta acacttatca agctttttat
    11281 acattaaaaa ataaaagtga gaaagattat tttcaaaatc ttgataataa tataatgata
    11341 attgcagata tcaaacttga agaagcagag gagagaaggc attttaatgg ctttgattat
    11401 cgtcagtatt taaaaagaca tggaatttat cgtatcgcca aagtgacaaa gataaaacag
    11461 atacgcttat ttcaacatag gtctttcttt gatcttatgt ttaagtggcg tagaagtgca
    11521 attgttatta gtcaaacttt tccaaatcct atgcgtcact atatgtcagg gcttttgttt
    11581 ggatatctag ataagacctt tgatgacatg tccgatttat atagtagtct aggtattata
    11641 catttatttg ctttgtcagg tatgcaagta ggtttttttc tcggtatttt tcgttatatc
    11701 tgtctacgta ttggcttacg tctagaccat gtttggttac ttcaaatacc attctcgcta
    11761 atttatgctg gtttaacagg ctttagtatc tcagtcgtta gggcacttat tcaatcttta
    11821 ttatcacata gcggtgtcaa gaaagatgag aactttgctc tctgcttgtt aatttgtctt
    11881 atctctctcc cccactcact tttgactacg ggaggagttc ttagctttgc ttatgctttt
    11941 atacttacga tgacctcctt tgatcatttt tcgagtataa aaaaagtagc tatcgaatct
    12001 ttgacagtct ctgtaggaat tcttcccata ctaacctact atttttcggg ttttcaacca
    12061 atatcaatta tattaacagc acttttatct tttgcatttg atattatatt tttgccttta
    12121 ttaactgtta tatttgtctt atcgcctatc gttaaattag gttgtattaa tagtttgttt
    12181 gaaatcctag aagtgttatt aaaatggact gggcaacttt ttccaaggcc acttattttt
    12241 ggaaagccca gcctttttct tttaatagtc atgattataa ttttgggatt actttatgat
    12301 tattatcatt ctaaatgttt tcgttattgc tcccttctta ttatctttac cttgtttttt
    12361 atcactaaga atccaattac taacgaggtt gcgattttag atgttggaca gggagatagt
    12421 attttagtga gggattggtt aggaaaaaca attttaattg atactggggg aagggtgaga
    12481 tttgaacagc ctgaagaatg gaaacaaaaa gtaaatcagt ctaatgctga gagaacgctc
    12541 attccttact tgaaaagcag aggtattagc aagatagatg atttagtgat aactcatacc
    12601 gatacagatc atatggggga tatggaagtt atctcaaagc attttaaagt tgcacgtttg
    12661 attacaagtt caggttcttt aacgaattcg cagtacgtta agcgtttatc aaagataggt
    12721 gtagcggtaa aatctataga agccggtgat aaacttgctg tcatgggaag ttatttacaa
    12781 gtactttacc catggcacaa gggtgatgga aaaaataatg attcaattgt tttatatgga
    12841 catttattag gaaaaggctt cttatttacc ggtgatttgg aggaagaggg agaaaagcag
    12901 ttattagaag cttatcctaa tttatcagta gatatcctta aagcaggaca tcatggttct
    12961 aagggctcat caagtctatc ctttctgaaa aagttgtctc ctagtgtggt tctagtgtca
    13021 gctggtaaaa ataatcgtta ccagcatcct catcaagaga ctttacaaag gttccaaaag
    13081 attaaaagca agattttccg aacggatcaa tcaggtacaa ttaggctaac aggatggtgg
    13141 aagtggcata ttcagacagt tcgttgattt cttaaaatga ttgcgtatct gttaaataca
    13201 atctattttt cgcaagaatt cagtaatttt aagcttgaca tggaacgggt tccagcattt
    13261 atactgattt cagaagaaga gaggtatttt ttatgactat caaacgtatt ttttgtgaca
    13321 tggatggtac actattaaat agtgaaggac aagtgagtaa aagtaacgct accttaatac
    13381 gtgaggcagc gattccagta actttagtgt ctgctagggc acctatggaa atgaaggatg
    13441 ccgtagatgc acttcaatta ggaggagtcc aagttgcttt taatggagga ttgatttata
    13501 gaataggaga taataatcaa gttctaccta ttcatacaca aatcattaaa aaaagcacag
    13561 taaaacaatt gctaagagga atacggtttc attttcctca agttagttta tcttattacg
    13621 atcttaataa ctggtattgt gataaaatag atgaaggaat ccgatacgaa catagtttaa
    13681 ctcaacaatg cccaacgttt attcataatg aggatcagtt tttagaaggt cacacaaata
    13741 cttttaaaat tatgatgatt actttcgatg aagcaaatat gctagaatta gaaaaatatc
    13801 ttcaaagttt ggagttacca gaaatcacta ttcaacgttc aggtaaagcg tatcttgaaa
    13861 ttacacatct cttagccaaa aaatctaaag gaattgctta catccttcaa aaagagcagt
    13921 tagctagaga agaaacggct gcatttggag atggacataa tgatttgcct atgcttgaaa
    13981 tggtaggtta ccctattgtt atggataatg cttttgatga tatcaaagcg atagcatatc
    14041 aactaactaa gtctaatgat gaagacggag tgggatatgg gattcaaaaa tttttaaagt
    14101 aggagtctta taatgacaag tattagtgat atagctaaaa aggcaggtgt tgctaaatca
    14161 acagtttccc gtgttatcaa tcatcaccca catgtttctg atgagacacg acaaaaagta
    14221 atggctctta tcaccgaatt ggattatata ccaaatcaac tagcgcggga tttaagtcga
    14281 ggcaaaacac aaaaaatagg tgtcgtcatt ccccacacgc gccatcctta ttttacacaa
    14341 ttaataaatg gcctattaga tgctgctaaa actaccgact atcaacttgt tatgatgcct
    14401 tcagattaca acaaagaatt agaactttcc tacttgaaac agctcaagat ggaagctatt
    14461 gatgctttaa tattcacttc aagagcaatt agcctcgata ttattgaaac ttatgcaaaa
    14521 tatggacgaa tagtagtttg tgaaaaattg caggaatata atcacctatc ctctgcttac
    14581 cttgatcgct attcttcatt tctagaggcg ttttctgata tgaagttacg aggtttggag
    14641 cacttagttt tattattttc aaggaataat gaatcctctg ctacctatca atccgcatta
    14701 ttagcttatc aagaggtcta tgggcaacta tcctctccat atatggtagt tgggaatgtt
    14761 catgacttta atgagggact aaatttatct tatcaattgg taaaagaagt tagtatagat
    14821 ggtatattag ctacaagtga tgaagtcgcg gcagggctaa taaaaggtta tgaagaaagt
    14881 aggaaaaaac gtccctatat tattggtcag gaatgtttat tagttgggca acttttgaaa
    14941 ctcccaacaa ttgaccacaa atcctattat ttagggaaat tagcttttaa acaggcttta
    15001 gctgaaaaga ttagtcaaga agttttattc tcagagtttt tatctcaaga caattagtca
    15061 ttcaaaagta atagaaaaaa ggtttccaac taaacttgat tggaaacctt tttatttaat
    15121 gattgataat tttaagcatt tgtttgaaag cagtctctgc ttcaggaatc ggtaagactg
    15181 gataaacgtg gttcattttt tggccaacga tatagtggtg tttaatgttt tcttttgcta
    15241 atttttgatg taataattga ttatcggggt agaaaatatc gtgcgttcct ataaaaaatg
    15301 atattggagc catcttgtta aattggctac tgtaaagagg actaacttga ggatttttaa
    15361 aattcttttt tccatttgcc cagtaaggag ctacttgttg taattgccaa gctttaagaa
    15421 ggggatcttt tttttgaact ttttcaatct ttggattatt agtagcaata tctaaccatg
    15481 gtgatattaa aataatttct tttggttgtt tgatatgctc cgtgacaagg ttatcagcaa
    15541 gacctaaagc aaggcctccg cctgcacttt cacctactag gataatctgt ttgtgagatg
    15601 tgacgctagc taatgtattt tggtataatt ttttaatttt ggggatagca tcactataat
    15661 tatatgtagg agctttaggg taaataggaa agacaacttt tgcatctaat tttttagcta
    15721 gtttattgac aaaaatatat tgtaattcgg aagcttgatg gatatatgat cctccatgaa
    15781 catagaagat aaccttttgc ttaggattat ttttatcatt ccaagtgaat gtttgcatgc
    15841 catcagtagc ggtaatttct accttttcat tatttaatgc ctgaggaatg tctacaggaa
    15901 gaacattacg tttagctacc ttagttaagt aattttggta ttctttaatg gtttgaggct
    15961 taatctgatt taccacaggt agagtttttt caatagtatt gctagttagg gatctttttt
    16021 caacaatatg cgtgtaagca tatgctcccg taaaaatgac aaaaaagatt agaagtagta
    16081 gtacaataag ttttcgatag tttttcatga ttatcctttt gtaattgagc tcgtcatgct
    16141 aaaaatgatt agttgttatt ctataataaa gtagatacga aaacaagaaa ataatgctat
    16201 aaaatttcaa cctattataa ttttttgctg atagtacgta ttaaccttta atttgctaaa
    16261 atagaagaga ggagagtgtt attatgtttc acagagaaga tttttcaaaa gcattattaa
    16321 aaaaagcttt gcagcccggt atgagagtta tggatattgg ttgtggtagt ggggaattaa
    16381 cgcgtttggc tgctgatatc gttggtaaag aaggtgatgt tgtaggaatt gatattaatg
    16441 agcaactttt atggcaagct agacatgata atggctatgc taatgttagt tatcaaaacg
    16501 cagatttagc agcattttcc tcctttgaaa caaaaagttt tgatgctatt attggacgtc
    16561 gtgtattaat gtatcaaaaa aatccaaaga aagtaataga aagtttaaca cctttgttaa
    16621 agcaaaatgg tttttatgtt tttcaagaaa gtgatgcaat gtgtttagaa ctagctaagc
    16681 ctagctttgt aaatatgaat aaaatacaat cactaatctg gcaaactgtt gagaaggaag
    16741 gaggacacat tcatattgga agtcaacttg agcaattaat gttagaagca ggcttaatcg
    16801 ttgaccattt tgtatcagaa aatgtcattc aaacgtcaca aactggtaca gatctatctt
    16861 ggctactaaa tatcatgaaa gaaagaatac aaaaatatag tgaccttcag gggttagaag
    16921 atgtcaattt acaaaaagaa ttagaagaga ataatgacca ttttatccga gattgtcaat
    16981 ttggaattat ttctcataga gttgatgaat gattttaagt attatttgac accttgaaaa
    17041 gtttccttat ggtataataa ggaatatgat tgcgattgaa gaaattggga gaattacccc
    17101 agataattta gggttagtaa ctgttttagc aggagaagat ttagggcaat atgctcagat
    17161 gaaagagaag ctatttcagg taattggatt caataaagac gatttagcct attcttattt
    17221 tgatttaagt gaagaagact atcaaaatgc ggaattagac ttagaaagtt tacctttttt
    17281 atcagactat aaggttgtta tttttgatca gtttcaagat attacgactg ataaaaaaac
    17341 ttatttagat gaacaagcaa tgaagcgctt tgaagcctac ttgcagaatc ctgtagatac
    17401 tacgagactt gttatttgtg ccccaggaaa acttgatgga aaacgtcgac ttgtaaagtt
    17461 attgaaacgt gatgctcgtg ttttagaggc taatacttta aaagaatcag atttaaagac
    17521 gtattttcaa aaatatgctc accaagaagg attagttttt gaagcaggcg tttttgatga
    17581 gttattgatt aagtctaact atgattttag tgatacttta acgaatatcg ctttcttaaa
    17641 gtcatataaa acagacggtc atatttcgtc taatgatgtt agagaggcta tccctaaaag
    17701 cctgcaagat aatatatttg atttgactca agatgtctta cttggtagga tagatttagc
    17761 gcgtgattta gtaagagatt tacgtctaca aggcgaagat gaaatcaaat tgattgcaat
    17821 catgttagga cagtttcgga tgtttttgca agttaaaata ttggctagta aaggaaagtc
    17881 agagagtcaa attgtttctg aactatccca ttacatagga cgtaaaatca acccttatca
    17941 agtaaaattt gctgtcaggg attctagaaa ccttcccctc gcttttttaa aagaagcgat
    18001 taggatttta attgagactg actatgctat taaaagaggg acttatgata aggattatct
    18061 ctttgactta gccttattaa aaattgctca taaaaagccc taattaaaga aacaaaagag
    18121 ttgaaagctg ttccaaattc ctatagaata agggtaataa tagatgaaaa gaggatttcg
    18181 acatggcaat tattttacca gatttaccct atgcatatga tgcgcttgag ccacatattg
    18241 atgctgagac aatgacacta catcatgata agcaccatgc aacttatgtt gctaatgcaa
    18301 atgctgctct tgggaaacat cctgaaattg gagaagactt agaagcgctc ttagctgatg
    18361 tttctcaaat tccagaagat attcgtcagg cagtcatcaa taacggtggt ggacatctta
    18421 accacgctct tttctgggaa ttgatgtcac cagaagaaac tcaaatttca caagagttat
    18481 ctgaagacat tgatgcaact tttggttcat ttgaagactt taaagctgct ttcacagcag
    18541 cagcaacagg acgttttggt tcaggttggg cttggcttgt tgttaatgct gaaggcaaac
    18601 ttgaagtgct ttcaactgcc aatcaagata ctccaattat ggaaggtaag aaacctattt
    18661 tagggcttga tgtatgggag catgcttatt acttaaacta tcgtaatgtt cgtcctaact
    18721 acatcaaagc tttctttgaa attattaact ggaataaagt aaatgaactt taccaagctg
    18781 ctaaagctta ataaaaaacg agtcgatagg ctcgtttttt tattattcta aaagttgata
    18841 atgtttttta aaagtctaga agtccaaact cagataaaac tagaacagtc ctattatctt
    18901 caactaagcg aataagttta ttgaaagcgc ttgacaagat tagtaagaaa tgataaaatt
    18961 aagacgtttt caggtgttat tatttaaggt aagcaagctg ataacagata aaattgcata
    19021 ataaaggatg ttatcacatt ttgtgagcag ggacctattc aacgacgact ttttgttgtg
    19081 tcttggatgg gtcatttttt attgtgcgag tgaaggacta ggactttaat gattataaaa
    19141 cgcgtattaa atcataatgc agtcatatca gtaacacatc aaggattaga tgtgttactg
    19201 atggggaaag gaattgcatt taagaaaaga ataggtgata ggattaatag cgatgctatt
    19261 gagaagtcat ttgttttaaa aaatagtgat aacatgaacc gttttacgga gttatttatt
    19321 actgttccag aagaagtagt tgcatgttca gaacgaatta tcaacctagg taaaatcaaa
    19381 ttgggtaaga acctagatga aattctttat atcaatttga cagatcatat tcattcggct
    19441 attgaaagac atgaacaagg aatggttatc caaaatccac taaggttgga aatccagcgt
    19501 tactatcctg atgaatactc cattgggatg aaggctctgg aacttataaa ggatgaattg
    19561 ggtatttgtt taacaattga tgagtcagct tttattgcta tgcactttgt aaatgctgga
    19621 ttggataatc cgtttaatga agcgcataaa atcactgaga ttgtatctta cattgaacag
    19681 aaagtaaaaa cagattttcg aacagaacta gatgaaagtt caattgatta ttatcgtttc
    19741 atgacacata ccaaattatt tgctcaacgt gttttatcag gaatgaaata cgaagatgat
    19801 gatgctgatt tacttttagt agttaagaaa aaatatccga gagaatataa atgtgttaaa
    19861 gagatcggca acaatatggc tatccaatac caatatcaac ttaattcaag tgaactccta
    19921 tatctgacgg tgcatgttaa acgattagtt aaaaatttaa aggagatatc agatgactaa
    19981 ataccaagag actgctaaag ctattttcgc agcagtaggg ggagaaaaaa atattcaaca
    20041 cgtaacccac tgtgtgacac gattacgatt agttttagac aatgatgaaa ttgtcaacga
    20101 tcaggttatt aaaactattc cgaatgttat cggtgttatg cgtaaaaatg atcaatacca
    20161 aattattttg ggaaatgatg tcaataatta ttataatgct tttttgacct taggtcattt
    20221 tgaaaatact accagagaat tttcatcaca aaaaaagagt tcaatacttg agaaattaat
    20281 tgaaacgatt gcaggagtca ttacgccgct tatacctgct cttctaggag gaggtatgct
    20341 caaggttata ggtattttgc tccttatgct cggtatagca agctctagtt ctcaaacagt
    20401 agcttttatc aatttttttg gtgatgccgc ttattatttt atgccaataa tgattgctta
    20461 ttcagctgcg tcacgattta aagttacacc tgtattagct gctacagtag gagggattct
    20521 cttacaccct gcttttgtga caatggtagc tgaaggaaaa ccattatctt tatttggagc
    20581 tccggttaca ctggctagtt atggttcttc tgttattcca atcttaatta tggtttttct
    20641 tatgcaatat attgagagat ggattaataa aattgttcct agtataatga agagtttttt
    20701 acaaccgact ttgattattc tcatttctgg ctttttagct ttagtggtcg tggggccact
    20761 gggggtaatt attggtaaag gattgtctag tgcaatgctt tcgatttacc atgtggcacc
    20821 atggtcagct ttagctattc ttggtgctat tatgccattg gttgttatga caggaatgca
    20881 ctgggcattt gctccaatat ttttagctgc ttctgttgcg acaccagatg ttttgatttt
    20941 accagcaatg ttagcttcta atttagctca aggtgcagct tcttttgctg ttgcagttaa
    21001 ggctaaacaa aaacaaacac gtcaggtggc ctttgcagca ggtttatcag cactactagc
    21061 tggtatcaca gaacctgcac tttatggtgt cacactgaaa ttcaagaagc ctttatacgc
    21121 tgcaatgata tcaggtggtt tagtaggagc ttatattgga ttagttaata ttgcttccta
    21181 tacatttgta gtaccatcta ttattggttt gccacaatac atcaatccac aggggggcaa
    21241 taatttcagt aatgctgtta ttgcagcaat agctactatt attttgactt tcattattac
    21301 atggttcttg ggaattgatg aaggagaaaa cgaaaaaagt agtattaatg ctcaagagca
    21361 cacacatatt agaagtggtt tatcaaaaaa agagacattg tattcaccta tggtaggcaa
    21421 tgtattacct ttatctaaag tacccgatga aacattttca tctaaattac taggtgaagg
    21481 tttagcaatc actcctagtg tgggtgaagt ttatgcacca tttgatagtg aaattataag
    21541 tttatttcct acaaaacatg ccattgcctt aaaggatgat aagggagtcg aagtgttaat
    21601 ccatattgga atagatactg tagaactaaa tggagaaggt tttgaacaat tggtcaaagt
    21661 tggcgatttt gttaaacgag gacagttact tttgcgaatg gatattgatt ttattagttc
    21721 aaaggggtat tctcttatta gtccggttgt agttacaaat tcaattgacc aacttgaaat
    21781 tatcgttaaa gacgaagaga caatggttac aaatgaagat gatttacttg taattcttta
    21841 aaaggaagga tgataatatg acggtttttc ctaaacattt tctatgggga ggtgcagttg
    21901 ctgccaatca ggtagagggt gcatttagaa cagatggcaa aggtttatct gtacaagatg
    21961 tacttccaaa tggtggttta ggcgatttca ccgccaaacc tactccagac aatttgaagc
    22021 tagaagcaat tgatttttat cataactata aaaatgatat aaaactgttt gcagagatgg
    22081 gctttaaagt ttttagaacc tctattgcct ggtctcgaat ttttccaaat ggtgatgata
    22141 gtgctccaaa cgaagctggc ttacaatttt atgataattt atttgacgag ttgcttaaat
    22201 ataatatcga gcccttggtt accttatctc attatgaaac accgcttcat cttgcaaaga
    22261 cctataatgg ttgggctgat agacgtttga tagcattttt tgagaaattt gctcaaacag
    22321 tcatggagcg ttataaagat aaggtaaaat attggttaac ttttaatgaa gtgaattcta
    22381 ttttacatat gcctttcacc agtggtgcta ttatgaccga caaatcacaa ctaagtcctc
    22441 aggagcttta tcaagctatt catcatgaat tagtagcatc ggctagagtg acaaaattag
    22501 ggcgttctat caatcctaat tttaaaattg gttgtatgat tctggctatg ccagcttatc
    22561 ctatgacatc tgatccaaga gatgtcctgg ctgcaagaca atttgagcaa cataatctgc
    22621 tattttcaga tatacatgtc agaggtaaat atcccactta tattcagtcc tatttcaaaa
    22681 ataatggtat taaaattaaa tttgaagaag gagatgagga agtattggca caaaatacag
    22741 tagattttct atcgtttagt tactatatga gcgtgacaca agcttatgac tttgaaaatt
    22801 atcaaagtgg tcagggaaat atccttggag gcctgactaa tcctcattta acaacttcag
    22861 aatggggatg gcaaatcgat cccatcgggc tacgcctagt cttaaatcag tattatgaac
    22921 gttatcagat tccgttgttt attgtagaaa atggattggg agcaaaagat caattgatag
    22981 aaacactaga cggtgattat actgtcgaag atgattatcg tattgattat atgaatcaac
    23041 atttagttca agttgctaaa gctattgaag atggcgttga aataatggga tatacatctt
    23101 gggggtgtat tgattgtgta tccatgtcta cagctcaact aagtaaacgt tatggtctaa
    23161 tatatgttga tcgaaatgat gatggaacag ggagtctaca acgttacaag aagaagtcat
    23221 ttggttggta ccaaaaagtg attaggacaa atggtcaatc attgtttgaa catcataaca
    23281 gataacatgt taaacaacta gtctctgttt caatttaata taaaaaggag tatccatagg
    23341 ttactccttt ttattataat ttatgaaggt taatatcttc tgatttaagt gctagataca
    23401 ataaagttgc atccttaaag tttcttgggt tgagttgcgt tgattcataa atcttattta
    23461 acctatattg aacagtattt ttatggataa aaagttcctg actacattct tttaatgata
    23521 gattatgctt aaaatatgag ttaagcaaga tcttatcatt ttcagataag tttacaagcg
    23581 ctttatttag cacatattgt ttaatattag aatcaatact tgttaataac agttcaagat
    23641 caaggtcgtc gacgaggttg accttttgtt gaccttttaa agctttcagg gctaagatag
    23701 acgtttcata tgatcttttt aaaagtgata aagatgattt ttgtccgata ccaactttat
    23761 agaggccctt tttgcattca aactttgata ggatatttgg ataataatag tcaaacatct
    23821 ggtcagaaag taaaagccag tattgattgg gataattaaa ggtataaata gcagtgtcaa
    23881 gtttatcaaa aaagttcaag agtgacattt cagattgact taagagtaat ttttgttttt
    23941 gactattgat ttcaaaagtg agtagacgat agtcagtttt ttctgatagg tggtattggt
    24001 ttaaaaattg attgaggtaa tagtagtcta actctctgcc ttcaacaaga tgatgtaaga
    24061 caatagaagc ttcgtttttt cgcccgaaat ccagataatc taattcatgc tcttttaaga
    24121 tgagtcgagt cattttttga gcaagtaaag catattttcc tacttggttg gggtttccac
    24181 taatcccaat agtagctaat aattcacaat tataatagaa aggaatattg atccctgctt
    24241 gtgtcccaaa ataagattct tgatctgtta cttcaatcat ttgtcctgtc tgtgctactt
    24301 ttaaaccaat ttcatggaat tctccaacac gttttggatt ggtactagcg aaaatgattc
    24361 ctttggtatt aataaaatta atatcttgtt gacaaacatc tttgatagag gtaacgattt
    24421 gttgtgcgag ttgttttctt aatttcattc agcttacctt ttgttatata taacaaatat
    24481 ca
//
LOCUS       DATRTN010000019        23532 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.019, whole genome shotgun sequence.
ACCESSION   DATRTN010000019 DATRTN010000000
VERSION     DATRTN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23532
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.019"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            170..787
                     /locus_tag="VBE22_000832"
     CDS             170..787
                     /locus_tag="VBE22_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202303.1"
                     /translation="MRKFFQDVIVEVAYYLEIALSVILATAIIFFSLALFHDLFSMLS
                     VDQNVDKLFQTFLARAMTIAVGIELIKMFSKPSPDTLIEVLLFALARQLVIEHGSVSD
                     YLMGIASVAILFAVRKYLFTHFDGSSNIVLRANNKVKKANILARVQLPCQPNETLGDL
                     LVRHLNDEEKDVAIGSVVYINNVALRVDSMHDGLVTRVEIIRSLH"
     gene            955..2394
                     /locus_tag="VBE22_000833"
     CDS             955..2394
                     /locus_tag="VBE22_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7202304.1"
                     /translation="MENFLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPLHDVFAG
                     FIKATVGYLILNVGAGGLVNTFRPILVALAKKFNLEAAVIDPYFGLASANAKLETMGF
                     ISVATTALLIGFGINILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQLRNG
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGGILAVLGPDIMSNVKLIGPGAFVPTKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVTELTNAFQGISNKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVVFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLAGGYHGNIDFEFPWLAFGYIFKYLGIAGYVIV
                     CLFFLAIPQLQFMKSKDKEAYYRGDAPAE"
     gene            2422..2700
                     /locus_tag="VBE22_000834"
     CDS             2422..2700
                     /locus_tag="VBE22_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7202305.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVSNFESASCSVGEA
                     KGLAANYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEIL"
     gene            2767..3252
                     /locus_tag="VBE22_000835"
     CDS             2767..3252
                     /locus_tag="VBE22_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7202306.1"
                     /translation="MNLKQAFIENDSIRLKLSASDWKEAIKLSIDPLIESGAVDAEYY
                     DAIIESTEEFGPYYILMPGMAMPHARPEAGVKRDAFSLITLTEPVVFPDGKEVSVLLA
                     LAATSSAIHTSVAIPQIIALFELENSIQRLTECQEAKEVLAMVEESKNSPYLEGLDLE
                     S"
     gene            3365..4030
                     /locus_tag="VBE22_000836"
     CDS             3365..4030
                     /locus_tag="VBE22_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEO7202307.1"
                     /translation="MTKRLPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQV
                     GSELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKEERGDRGEIQIELYGDWTYEQAQQWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLSTDTLQLFEGVDVFTFIAGRGITEADDPAAAARAFKDEIKR
                     IWG"
     gene            4034..4897
                     /locus_tag="VBE22_000837"
     CDS             4034..4897
                     /locus_tag="VBE22_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEO7202308.1"
                     /translation="MTRPIGIYEKATPKHFNWLERLQFAKELGFDFVELSIDESDERL
                     ARLEWSKEERLELVKAIFETGVRVPTITFSGHRRFPMGSNNPEKEARAMDMMKKCIVF
                     AQDIGIRNIQLAGYDVYYEEKSPETRARFIKNLRQACTWAEEAQVILSIEIMDDPFMN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDLWSEFYNGHRSIAALHIKDTYAVTET
                     SKGQFRDVPFGQGCVDWEEMFAVIKKTNYNGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLMEKTGVK"
     gene            4899..5615
                     /locus_tag="VBE22_000838"
     CDS             4899..5615
                     /locus_tag="VBE22_000838"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEO7202309.1"
                     /translation="MAKSLQEMRERVCEANKSLPVHSLVKFTWGNVSEVDREAGLIVI
                     KPSGVDYDQLTPENMVVTDLEGNIVEGDLNPSSDLPTHVQLYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLSEDEVNTAYEKETGSVIIEEFERRDL
                     DPMAVPGIVVRNHGPFTWGKDPAQAVYHSVVLEEVAKMNRFTEQINPRVEPAPKYIMD
                     KHYLRKHGPNAYYGQKGQEH"
     gene            5626..6273
                     /gene="fsa"
                     /locus_tag="VBE22_000839"
     CDS             5626..6273
                     /gene="fsa"
                     /locus_tag="VBE22_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO7202310.1"
                     /translation="MKYFLDTADVSEIRRLNRLGIVDGVTTNPTIISREGRDFKEVIN
                     EICQIVDGPVSAEVTGLTCDEMVTEAREIAKWSPNVVVKIPMTEEGLAAVSQLSKEGI
                     KTNVTLIFTVAQGLSAMKAGATFISPFVGRLEDIGTDAYALIRDLRHIIDFYGFQSEI
                     IAASIRGLAHVEGVAKCGAHIATIPDKTFASLFTHPLTDKGIETFLKDWDSFKKK"
     gene            6437..7420
                     /locus_tag="VBE22_000840"
     CDS             6437..7420
                     /locus_tag="VBE22_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7202311.1"
                     /translation="MFEKVTISDIANLVGVSKATVSYYLNGNYKKMSLQTKEKIRLAI
                     KETGYQPSKIAQSLVTKNTRTIGVVIADITNPFISSVMKGIHDTCQQFGYSVNFTNSD
                     NDIDIELENLNRLNQQNVSGIILDSVDPNHSFIETLSNDRLVMVDRQAKDIKVDTVAS
                     DNKESTQIFLEKMQEAGYHDIYFVTYPIEGISTRELRYEGFKEVVSSNPDKLIIITED
                     GSTQRILDIIEHSEQKPGFLMMNGPTLLNFMKKLNQSTVSYPEDYGLGSYEDLEWMQV
                     LTPNVSCIKQDSYGIGCLAAQCLIEKISQGNEPTTARLLEVKNQIVIRQSF"
     gene            7655..8674
                     /locus_tag="VBE22_000841"
     CDS             7655..8674
                     /locus_tag="VBE22_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202312.1"
                     /translation="MKINKLLSDRLEACYSVCRLQNQDYDFLLVASEVDQACFAYDLN
                     SDLAKIVVWEDVGGTMSIIPIPGTMDFLATQKFYPGFKAKDCQVVYASFQGDHWDICN
                     AFDFPYLHRFDLIKGKENQLYFIGCTIANSKAHTEDWSDKGRIFVARFNTQNHTLEGL
                     QQLPQTLLKNHGYYAIQDEGYSLITSVEGVFKLTYPEFSTTGDWQLERLFDEETSDVV
                     KVDINQDGKDEYVIIQGFHGDRLRIFTEDFGRELFHYPEKTPFGHAIWSGRLLNQTCF
                     VFGWRSEKAELRLFHFVDGHLVSELVDAKAASSNVLAFEKDGKAYLFSANNGRGEVAL
                     YQLVK"
     gene            8693..9649
                     /locus_tag="VBE22_000842"
     CDS             8693..9649
                     /locus_tag="VBE22_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO7202313.1"
                     /translation="MDKKKILVTGTVPKEGLRKLMDRFDVTYSEDRPFSRDYVLEHLS
                     EYDGWLLMGQKGDKEMIDAGENLQIISLNAVGFDHVDTAYAKEKGIIVSNSPQAVRVP
                     TAEMTFALILAASKRLAFYDSIVRSGEWIDPSEQRYQGLTLQGSTLGIYGMGRIGLTV
                     ANFAKAFGMTVVYNDVYRLPEDKEKELGVTYLEFDQLIKTADVITIHAPALPSTIHKF
                     NKDVFAKMKNRSYLINAARGPIVSEEALIEALKEGEIAGAGLDVFENEPQVSEGLRSL
                     DNVIMSPHAGTGTIEGRRTLAEEAADNIIAFFDGKPQNIVNK"
     gene            9747..11198
                     /locus_tag="VBE22_000843"
     CDS             9747..11198
                     /locus_tag="VBE22_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO7202314.1"
                     /translation="MLEALHKFFEVFGAATVVPAMIFIVSLFLKVNPRRAFFSALRAG
                     VGLTGFGWIISAFAPMVTKYIQQMVKTTGLHLPIVDIGWQAGSLTAFSSEIGLSFFVF
                     GLLIELGLFLLGITRVFVPSNLWNNFGYMIWGTMAYAATGNFILSFAFMVFVLLYSLV
                     MSEVLADRWSEYYGVKNATINSIHNIETLIPALILDPLWNLLGVNKVKLNPESLKTKL
                     GIFGEPMTLGFILGVIIGVLGSLRNLASIDTWGGILGFAVALAAVMTIFPLITGVFAS
                     AFAPLAEAVERNKKKESQAEQGALDKKRWFIAVDDGVGFGEPATIIAGLILVPIMVVI
                     SLILPGNEALPVVDLIAIPFMIEAMIAVSKGNILKAILNGIIWFSLGLYAASALGPIY
                     TEAVKHFGTALPAGVTLIMSFNLLAHPLTALVFFAWISGNPLYIGVTVVIYLAFLFLL
                     RTHRESIYAYLKTMADKNSGYEGSCEKIFIAED"
     gene            11220..12209
                     /locus_tag="VBE22_000844"
     CDS             11220..12209
                     /locus_tag="VBE22_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO7202315.1"
                     /translation="MKKIDGHAHIVDTIAGFNGKGRLNALGNGYAIWDDGDLIKLIPD
                     EYGDKAFTAEAFLKYMDANGVDKAVILQGHLNGYQNYYTHLAIKRYPERFTGAFSVDP
                     FADNAMQIVKRHVEVLGFRAIKFEISQGGGIHGYRGQKTPFRLDTDPHVSRILTYLLD
                     YPGFVVTVDYGNWDQISHQPDAIANLARLYTSLDFVVCHLSFPHVEHSNRLRAELNMW
                     KNFNNIYTDISAIQDIDAPDSFPFPKSEQNVRIAKEVLGAKRIIWGTDSPWSATFNTY
                     EELATWLENVDIFSQEELEDVMYNNAERVYFKEEHVKANRAGIDDTTKELNLY"
     gene            12275..13792
                     /locus_tag="VBE22_000845"
     CDS             12275..13792
                     /locus_tag="VBE22_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEO7202316.1"
                     /translation="MTYYLSIDYGGTNTKALIFDKLGHQIAVSSFETLKNETQSGHRQ
                     VNLVKTWNAITSAIREVIQISKLSPEQISAVACIGHGKGLYLLDNKLEPLEQGILSTD
                     NRAKDLAQYFESKLDNIWELTRQHIFPSQSPVILRWLKDYQPETYKSIGAVLSAKDFI
                     RYKLTGKVQQEYGDASGNHWINFQTGTYDPAILDFFGIREIENSLPELIDSADLVPGG
                     ISSQAAKETGLVEGTPVVGGLFDIDACALGSGVLESDTFSVISGTWNINTYPSLKPAK
                     QDSGLMTSYFPDRRYLLEASSPTSAGNLNFMLKMLMHQEIDNAKSSGGSIYDNLEEFL
                     THTDATHHGLIFFPFLYGSNTSQDASACFFGLTTKSTKSQMIRAVYEGIAFAHKQHIT
                     DLIKSRGSVPKIIRFSGGATNSPAWMQMFSDILNFPIETVEGTELGGLGGAILARHAL
                     DKISLKEAVQDMVRVKAIYKPQLSEVKGYKKKYHAYQKLLETLDPIWSELGHLNK"
     gene            13812..14573
                     /locus_tag="VBE22_000846"
     CDS             13812..14573
                     /locus_tag="VBE22_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HEO7202317.1"
                     /translation="MKQEDIIINSIVFKKQLDKGFTQVQLIKLVKDLGFSRFEIRQEL
                     LQDPDRELPALKAEADFYDINLYYSANEDLIKGGKVNPYLNKGLKEASQLGAPFIKLN
                     VGQTRNLSKEELEPLKEILKSQTIGIKVENNQDPKAATVENCQYFMTLVKELQIPISF
                     VFDTANWAFINQDLYQAVNSLACDTTYLHCKNFLQVAGKPHLSKSLFEGEIDLTDLLK
                     SFSNCEYLALEYPTELEVLKRDVQRLISISNSQNF"
     gene            14639..16318
                     /locus_tag="VBE22_000847"
     CDS             14639..16318
                     /locus_tag="VBE22_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEO7202318.1"
                     /translation="MIILDKKSYDLLFYLLKLEEPETVMAIANALNQSRRKVYYHLEK
                     INDALPSDVPQIVSYPRVGILLTEKQKAACRLLLDEVTDYSYVMKSSERLQLSLVSIV
                     VAKDRITIDRLMQLNDVSRNTILNDLNELRSELAEKEYNLQLQSTKCRGYFLDGHPLS
                     IIQYLYKLLDDIYHNGSSSFIDLFNHKLSQAFGASTYFSKEVLDYFHHYLFISQRSLG
                     KKINSQDGQFMIQILPFILMAYRKMRLSPEVQTSLNSDFSLVWQRKEYEIAKELADEL
                     EENFQLSLDEIEVGLVAMLMLSFRKDRDNHLESQDYDDMRATLTSFLKELEERYHLHF
                     VHKKDLLRQLLTHCKALLYRKRYGIFSVNPLTEHIKDKYEELFAITSSSVKLLEKAWQ
                     IKLTDDDVAYLTIHLGGELRNSQQSPNKLKLVIVSDEGIAIQKLLLKQCQRYLTNSDI
                     EAVFTTEQYQSVSDLMHVDMVVSTSDALESRFPMLVVHPVLTDDDIIRLIRFSKKGNC
                     ANSNQFTNELEKTIAQYVKEDSERYVLKSKIEKLIHQELLQDVLPLQSTVC"
     gene            16635..17726
                     /gene="ulaG"
                     /locus_tag="VBE22_000848"
     CDS             16635..17726
                     /gene="ulaG"
                     /locus_tag="VBE22_000848"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEO7202319.1"
                     /translation="MPNVKDITRESWILSTFPEWGTWLNEEIEEEVVADGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVI
                     DPFAINELDYYLVSHFHSDHIDINTAAAIINNPNLDHVKFVGPYECGEIWKKWGVPEE
                     RIIVIKPGESFEFKDIKVTAVESFDRTCLVTLPVDGAEEHDGELAGLAVTDEEMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDYKIDVAINNYGDNPVGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DKDRIEYHHPRGFDDCFEQESNIQFKALL"
     gene            17816..20194
                     /locus_tag="VBE22_000849"
     CDS             17816..20194
                     /locus_tag="VBE22_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEO7202320.1"
                     /translation="MSEFDTKSYLEKLDAWWRAANYISAAQMYLKDNPLLRRELVEND
                     LKVHPIGHWGTVPGQNFIYAHLNRAINKYDLDMFYIEGPGHGGQVMVSNSYLDGSYTE
                     LNPNIEQTEDGFKQLCKIFSFPGGIASHAAPETPGSIHEGGELGYALSHATGAILDNP
                     DVIAATVIGDGEGETGPLMAGWLSNTFINPVNDGAVLPIFYLNGGKIHNPTIFERKTD
                     EELSQFFEGLGWKPIFADVVELSEDHAAAHALFAEKLDQAIQEIKTIQSEARQKPAEE
                     AIQAKFPVLVARIPKGWTGPKAWEGTPIEGGFRAHQVPIPVDAHHMEHVDSLLSWLQS
                     YRPEELFDESGKIVDEIAAISPKGDRRMSMNPITNAGIVKAMDTADWKKFALDINVPG
                     QIMAQDMIEFGKYAADLVDANPDNFRIFGPDETKSNRLQEVFTRTSRQWLGRRKPDYD
                     EALSPAGRVIDSQLSEHQAEGFLEGYVLTGRHGFFASYESFLRVVDSMVTQHFKWLRK
                     SKTHTTWRKNYPALNLIAASTVFQQDHNGYTHQDPGILTHLAEKTPEYIREYLPADTN
                     SLLAVMDKAFKAEDKINLIVTSKHPRPQFYSIAEAEELVAEGYKVIDWASNVSLNQEP
                     DVVFAAAGTEPNLEALAAISILHKAFPELKIRFVNVLDILKLRHPSQDARGLSDEEFD
                     KVFTTDKPVIFAFHSYEDMIRDIFFSRHNHNLHTHGYRENGDITTPFDMRVMSELDRF
                     HLAQDAALASLGNEAQAFSDEMNQMVAYHKDYIREHGDDIPEVQNWKWENIK"
     gene            20571..21794
                     /locus_tag="VBE22_000850"
     CDS             20571..21794
                     /locus_tag="VBE22_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO7202321.1"
                     /translation="MTNILEVKNLTKIFGKKQKAALEMVKQGKSKTEILEKTGATVGV
                     YDASFEIKEGEIFVIMGLSGSGKSTLVRMLNRLIDPSSGNIYLDGKDIAKMNVEDLRN
                     IRRHDINMVFQNFGLFPHRTILENTEFGLEMRGVSKEERTMLAEKALDNAGLLPFKDQ
                     YPSQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDTNKQTI
                     IFISHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTVLEIDGPQVALTRMHREEVSMLMATNRRRQLLGSLTADAAIEARKKGL
                     PLSEVIDKDVVTVSKDTVITDIMPLIYDSSAPIAVTDDNDRLLGVIIRGRVIEALANV
                     QDETVVESPKETVEA"
     gene            21813..>23532
                     /locus_tag="VBE22_000851"
     CDS             21813..>23532
                     /locus_tag="VBE22_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEO7202322.1"
                     /translation="MENLLQHKLPVAPFVESTTNWITKTFSGLFDFIQTIGNALMDWM
                     TKTLLFINPLLFIVLITVAVFFLAKKKWQLPTFTFIGLLFIYNQGLWEQLINTFNLVL
                     VASLISIIIGVPLGIWMAKSDKVKQVVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVVFALPPTVRFTNLAIREIPLELIEASDSFGSTAKQKLFKVELPLAKNTIMAGIN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGTGFVSGLSLVILAIVLDRVSQFF
                     NSKPGEKQAKTSKVKKWVGLGALVLFILAALGRIVVNMTSGNEAKGQKVKIAYVQWDS
                     EVASTNVIAEVLKSKGYDVELTPLDNAVMWQTVANGNADFTTSAWLPKTHGQYFNKYK
                     NSLDDLGPHVENVKIGLVVPKYMNVNSIEELSNQADKQITGIEPGAGIMKSAKQSLKD
                     YPNLSSWKLLSASTGAMTTTLGKAIKNKDQVVITGWSPHWMFAKYDLKYLKDPKKSFG
                     GEEHINTIARKNLKKDMPKVYKIIDKFKWTKEDMESIMLDMDKGMEPAKAAQKWIKNH
                     KKEVSEW"
BASE COUNT         7428 a         3882 c         4741 g         7481 t
ORIGIN      
        1 tttcctccca cttatttacc aataagctta ttatatcata atgtgtaatc aaacattcgt
       61 tagtactgat taaaaggtta atgaaggggt ggtcactatt gtttcattga attagtaact
      121 ttaacgacga aatgttataa tgttattagt ttataaaaga ggagattgta tgcgtaaatt
      181 tttccaagat gttattgtag aggtggctta ttatttagag attgctttgt cagttattct
      241 ggcaacagct attatttttt ttagtttagc actctttcat gatttgttta gcatgttgtc
      301 tgttgatcaa aatgttgata agttgttcca gactttcctt gctcgcgcca tgacaatagc
      361 agtcgggatt gagttgatca aaatgttttc aaaaccttcc ccagatactt taattgaagt
      421 gttattgttt gccttagctc gtcaacttgt aattgaacat ggtagtgttt cagattacct
      481 tatggggatt gcttcagttg ctattttgtt tgctgttcgt aaatatcttt tcacccattt
      541 tgatgggtca agcaacattg ttttacgtgc taataacaaa gtcaaaaaag ctaatatttt
      601 agctagggtt caactacctt gtcaacccaa tgaaacttta ggagacttac ttgttcgtca
      661 tttaaatgat gaggaaaagg acgttgctat tggttcagta gtctatatta ataacgttgc
      721 attgcgtgtt gatagtatgc acgatggtct tgtgacgcgt gtagaaatca ttaggagtct
      781 acattaaaaa atataaaaaa tcatgtatcc taagatacat gatttttttg taaattaggt
      841 tcagcacaat tttgcacact taagtgtgta cttcttggtc ttttttaaaa cgcttacaaa
      901 aaatataata aagatacaaa aaaacattac ttaaataaag agaggtcttt agttatggaa
      961 aacttcctag ctccccttaa ttggttctcg caaaatatct tgcagaatcc agccttcttc
     1021 gtaggtctat tggtactcat tgggtatctt ttattgaaga aaccacttca tgatgtcttt
     1081 gctggtttta tcaaagcgac cgttggatat ttgattttga acgtaggtgc aggaggattg
     1141 gttaatacat tccgtccaat cttagttgct cttgctaaaa aatttaattt agaagctgct
     1201 gttattgacc cttactttgg tttagcatca gcaaatgcta agttagaaac aatgggcttt
     1261 atcagtgttg caacaacagc actcttgatt ggttttggga ttaacattct cttggttgct
     1321 cttcgtaaag taactaaagt ccgtactctt tttattactg gtcatatcat ggtacaacaa
     1381 gctgcaacaa tttcagtatt tgtactgctc ttgattccac aacttcgtaa tggttttggt
     1441 gcatgggctg ttggtatcat ttgtggtcta tattgggcgg taagttctaa tatgaccgtt
     1501 gaagcaactc aacgccttac aggtggtggc ggatttgcca tcggacacca acaacaattt
     1561 gcaatttggt ttgttgataa agttgcgcca ttcttcggta aaaaagaaga aaaccttgat
     1621 aacttaaaat taccaacatt cttaaatatt ttccatgata cagttgttgc atcagcaaca
     1681 ttgatgctag ttttctttgg aggaatctta gctgtccttg gtccagacat tatgtctaat
     1741 gttaaattaa tcggacctgg tgcatttgta ccaactaagc aagctttctt catgtacatc
     1801 cttcaaacat cattgacatt ctcagtttac cttttcattt tgatgcaagg tgtacgcatg
     1861 ttcgttacag aattgacaaa tgccttccaa ggtatctcaa acaaacttct tccaggatca
     1921 ttcccagcag ttgacgttgc agcttcatat ggttttggtt cttcaaatgc agttctttca
     1981 ggatttgctt ttggtttgat tggtcaatta attacaatcg ctctacttgt tgtcttcaaa
     2041 aatccaatct tgattatcac aggattcgtt ccagtattct ttgataatgc ggcaatcgct
     2101 gtttatgcag ataaacgtgg tggctggaaa gcagcagtag ctctatcatt tatctcaggt
     2161 attattcagg tggcacttgg tgctgttgct gtaggtcttt taggacttgc aggtggttat
     2221 catggaaata tcgactttga attcccatgg ttagcatttg gttacatctt caagtattta
     2281 ggtatcgctg gttacgtgat tgtatgtctc ttcttcttag ctattccaca acttcaattt
     2341 atgaaatcta aagataaaga agcatactat cgtggtgatg cgcctgcaga ataatagaaa
     2401 tttataacgg aggaaataaa aatggttaaa gttcttacag catgcggaaa cggcatggga
     2461 tcatcaatgg ttatcaaaat gaaagtcgaa aatgcgcttc gtcaattagg cgttagcaac
     2521 tttgagtcag catcttgctc agtcggagaa gcaaaaggtt tagctgcaaa ctacgatatc
     2581 gtggttgctt caaaccacct tatccatgaa ttagacggac gtacaaaagg tcatttagta
     2641 ggtcttgata accttatgga tgacaatgaa ataaaaacaa aactacaaga aattttgtaa
     2701 gttaatagac aaagggagtg agacaaaagt ttctctccca tatctttaga cttttaagga
     2761 gaaatcatga atttaaaaca agcctttatc gaaaatgact caattcgtct taaattatct
     2821 gcaagcgatt ggaaagaagc aatcaaatta tcaattgatc cattgattga aagtggtgct
     2881 gttgatgcag aatattacga tgccattatt gaatctacag aggaatttgg tccgtactac
     2941 attcttatgc cgggtatggc gatgcctcat gctcgtccag aagcaggcgt aaaacgtgat
     3001 gcattttcat taattacatt aacagagcca gtagtttttc cagatggcaa agaagtctca
     3061 gtattgttag cacttgctgc aacaagttca gctattcata cttctgttgc tatccctcaa
     3121 attattgccc tttttgaatt ggaaaattca attcagcgct tgacggaatg ccaagaagca
     3181 aaagaagtcc ttgcaatggt agaggaatca aaaaacagcc catatttaga aggtttagat
     3241 ttagaaagct aatcaataaa ctgatattaa tagaaaggat aggacctata aaaaatctct
     3301 gtgataagaa actctttgcc acaaataaaa tgagtatttt tatcaggtcc ttagaataaa
     3361 caacatgaca aaacgtttac caaatttaca agttgcactt gaccactcag atttacaagg
     3421 agctatcaaa gctgccgtat cagtaggaca tgaagttgat gttattgaag ctggaactgt
     3481 ttgccttctt caagtaggta gtgaattagt tgaggtcctt cgcagtctct tccctgacaa
     3541 aataattgtt gctgatacaa aatgtgctga tgctggtggg actgttgcta aaaataatgc
     3601 tgttcgcggt gcagactgga tgacttgtat ctgttgtgct actattccaa caatggaagc
     3661 agccttaaaa gctatcaaag aagaacgcgg cgatcgtgga gaaattcaaa tcgaactata
     3721 tggtgattgg acttatgagc aagcgcaaca atggttggat gcaggtattt cacaagccat
     3781 ttatcaccaa tctcgtgatg cgcttcttgc tggcgaaaca tggggagaga aagaccttaa
     3841 taaggttaaa aaattgattg atatgggctt ccgagtttct gtaactggtg gtttatcaac
     3901 tgatactctt caattatttg aaggtgttga tgtctttact tttattgcgg gtcgtggtat
     3961 tacagaggca gatgatccag ccgcagcagc acgtgcattt aaggatgaaa ttaaacgtat
     4021 ttgggggtaa tatatgacac gtccaatagg aatttacgag aaggcaacac caaaacattt
     4081 taactggtta gaacgcctac aatttgctaa agaattggga tttgattttg tagaattatc
     4141 cattgatgag tcagatgaac gtttggcacg tttggagtgg tcaaaagaag aacgacttga
     4201 gttggtcaaa gctatttttg agacaggagt tcgtgttcca acaattacat ttagtggtca
     4261 tcgtcgtttc ccaatgggat ctaataatcc tgagaaagaa gcgcgtgcga tggatatgat
     4321 gaaaaaatgt atcgtctttg ctcaagatat tggtattcgt aatattcaat tagcaggata
     4381 tgatgtgtac tatgaagaaa aatctccaga gacacgtgca cgctttatta agaatcttcg
     4441 tcaagcttgt acttgggccg aagaagcaca agttattcta tctattgaga ttatggatga
     4501 tccatttatg aattccattg agaaatattt ggctgtggaa aaagagattg attctcctta
     4561 tctctttgtc tacccagata ctggaaatgt atcagcatgg cataatgatc tttggagtga
     4621 attttataat ggacatcgct caattgcagc gcttcatatt aaagatactt atgctgttac
     4681 cgaaacgtct aaaggtcaat tccgtgatgt tccatttggt caagggtgtg ttgattggga
     4741 agaaatgttt gctgtcatta agaaaacaaa ttataatggt ccattcttga ttgaaatgtg
     4801 gtcggagaac tgtgagactg tagaggaaac acgtgcagcc attaaagagg cgcaagattt
     4861 tctttaccca ttaatggaga aaacaggagt gaaataatat ggcaaaatca ttgcaagaaa
     4921 tgcgtgaacg tgtgtgtgaa gctaataaat cactaccagt tcatagttta gttaaattta
     4981 cttggggtaa cgtttctgaa gttgaccgcg aagcaggatt gattgttatc aaaccttcag
     5041 gagttgacta tgaccaattg acacctgaaa atatggttgt cacagactta gagggaaata
     5101 tagtggaggg ggatttgaat ccatcatctg atcttccaac tcatgttcaa ctttataaag
     5161 catggccaga agtcggtggt attgttcata ctcattcaac agaagcagtt ggttgggcac
     5221 aagcgggtcg cgatattcca ttttatggaa caacacatgc agattatttt tatgggccag
     5281 taccatgtgc taggtcactt agtgaagatg aagttaacac tgcttatgaa aaagaaacag
     5341 gtagcgttat tatagaggaa tttgaacgtc gggatttaga tccaatggct gttcctggta
     5401 ttgttgtacg taatcatgga ccatttactt gggggaaaga tccagcacaa gcagtttatc
     5461 actcagtcgt tcttgaagaa gttgctaaga tgaatcgttt cactgaacaa attaatccac
     5521 gtgttgaacc agctccaaaa tatattatgg ataaacacta tttacgtaag catggaccaa
     5581 atgcttatta tggtcaaaaa ggacaagaac attaaggaga ataatatgaa atacttttta
     5641 gatactgccg atgtttctga aattcgtcgt cttaatcgtt taggaatcgt tgatggtgtt
     5701 actaccaatc cgactattat ttcacgtgag gggcgtgact ttaaagaagt tattaacgaa
     5761 atttgccaaa tcgttgatgg cccagttagt gcagaagtga caggtttaac ttgtgatgaa
     5821 atggttaccg aagctagaga aatcgctaag tggtcaccaa atgttgttgt taaaattcca
     5881 atgactgaag aaggtttagc agcagtttca caattatcaa aagaaggtat aaagacaaat
     5941 gtgacattaa tatttactgt ggctcaaggt ctttcggcaa tgaaagctgg tgcaacattt
     6001 attagtcctt ttgtgggtcg tcttgaagat attggaacag atgcttatgc tttgattcgt
     6061 gaccttcgtc acattattga tttttatggt ttccaatctg agattattgc tgctagcatt
     6121 cgtggattgg cgcatgtgga gggcgtcgct aaatgcggtg cgcacattgc cactattcca
     6181 gataaaacat ttgcatcact atttactcac ccgcttactg ataaagggat tgaaactttc
     6241 ttaaaagatt gggatagttt caagaagaag tagtgttacg ataaagcaaa ctcttaatta
     6301 tactagtgca agacaaactt tgataatggg tttgtctttt tttataatct tctccaataa
     6361 gaacacttat tttcattttt cttattaaat gatataatac ggataaatta agtctaatta
     6421 gaataaggaa atagagatgt ttgagaaggt aaccattagt gatattgcaa atttagtagg
     6481 tgtgtcaaaa gctacagttt cctattatct caatgggaac tataagaaaa tgtctctgca
     6541 aacgaaagaa aaaatacgtt tagctattaa ggagactggt tatcaaccaa gtaagattgc
     6601 tcaaagttta gttacaaaaa atacccgtac tattggggtc gttattgctg atattactaa
     6661 tccctttatt tcgtctgtga tgaagggaat acatgatact tgtcaacaat ttggttactc
     6721 ggttaacttt actaactcgg ataatgatat tgacattgag ttggaaaatt taaaccgttt
     6781 aaatcagcaa aacgtttcag gaattatttt agattctgta gatcctaatc attcatttat
     6841 agaaactttg agtaatgatc gtcttgtcat ggttgataga caagctaaag atattaaagt
     6901 agatactgta gcatccgaca ataaagaatc gactcaaata tttcttgaaa aaatgcaaga
     6961 agctgggtat catgacatct attttgtcac ttatcccatt gaagggattt caacccgtga
     7021 gcttcgttac gaaggtttta aagaagtggt cagttcaaat cctgataagt tgattattat
     7081 cactgaagat ggtagtacac agagaattct tgatatcatt gaacattcag agcaaaaacc
     7141 agggttctta atgatgaacg gtccaactct tttgaatttt atgaaaaaat taaatcaatc
     7201 aacggtcagc taccctgagg attatggtct tgggagttat gaagatttag aatggatgca
     7261 ggtgcttaca ccaaacgttt cctgtataaa acaggattca tatggcattg gttgcctggc
     7321 tgcccaatgt ttaattgaaa aaattagtca aggaaacgaa ccaactactg caaggttact
     7381 agaagtaaaa aatcaaatcg ttattcgtca atctttttaa aaatgtttgc gagagcaagc
     7441 atttttttat aaaaaagcaa aaaacactat gtaaacgctt gcttttatag aaaatgatag
     7501 tataatagaa ttataaatga gttaaccgtt taacttaaga ataggtaagc ttttaacgaa
     7561 ccatctctaa ctctcttaac tcaaatgttt ttattttttt gcaccattag ttaaacggtt
     7621 aactcaagtt ttatcttcac caaaggagga tagaatgaag attaataaat tattgtcaga
     7681 cagacttgaa gcttgttatt cagtttgtcg tttgcaaaat caagactatg attttttatt
     7741 agtagcttca gaggttgacc aagcctgctt tgcttatgat ttgaatagcg atttagcaaa
     7801 aattgttgtt tgggaagatg tgggaggtac catgagtatt attccaattc ccggaaccat
     7861 ggattttttg gcaacccaga aattttaccc agggtttaag gccaaagatt gtcaggttgt
     7921 ttatgcaagt tttcaaggag atcattggga catttgtaac gcatttgatt ttccgtattt
     7981 acatcgcttt gatctcatta aaggtaaaga aaatcaactt tactttatag gttgtacaat
     8041 tgctaacagt aaagcccaca ctgaggattg gagtgataaa ggccgaattt ttgttgctcg
     8101 ttttaatact caaaaccata cattggaagg attgcaacaa ttgcctcaaa ctttattaaa
     8161 aaatcatgga tactatgcca ttcaggatga aggatattca ttgattactt cagtagaagg
     8221 ggtattcaaa ctcacttatc cagaattttc tactacaggc gactggcaat tagaacggct
     8281 tttcgatgag gagacaagcg atgtggtgaa agtggatatt aatcaggatg gcaaggatga
     8341 gtatgtgatt atccaaggtt ttcatggaga tcgtttacgc atcttcactg aagatttcgg
     8401 tcgagaatta ttccattatc cagaaaaaac accatttggt catgctattt ggagtggtcg
     8461 tttacttaat cagacttgtt ttgtattcgg gtggcgatca gaaaaagcag aattaaggct
     8521 ttttcacttt gtagatgggc acttggtttc agaattagta gatgcaaaag cagcttctag
     8581 taatgtctta gcttttgaaa aagatggaaa agcttatctt ttctcagcca ataacggacg
     8641 tggcgaagtt gctctttatc aattagtaaa ataaatttaa gaaggagaca atatggacaa
     8701 gaaaaaaatc ttagtaacgg gtactgtgcc taaagaaggt ctaagaaagc ttatggaccg
     8761 atttgatgtt acttattcag aagatcgccc attttcacgt gactatgtgt tagagcattt
     8821 atctgaatat gacggatggt tactcatggg acaaaaaggc gataaagaga tgattgatgc
     8881 aggtgaaaac ttacaaatta tttctttaaa tgcggttggt tttgatcatg tagatactgc
     8941 gtatgctaaa gaaaaaggaa ttatagtctc taattcccct caagcagtac gagttccgac
     9001 agcggaaatg acttttgctt taattttagc tgccagtaag cgccttgcat tttatgatag
     9061 tattgtaaga agtggagagt ggattgatcc gagcgaacaa cgttatcaag gtttgacttt
     9121 gcaaggttca acattaggta tttacggaat gggacgcatc ggtttgacag tcgcaaattt
     9181 tgctaaagca tttgggatga ctgttgttta caatgatgtt tatcgacttc ctgaagataa
     9241 ggaaaaagag cttggtgtaa cctatctcga atttgatcag cttattaaaa cagctgatgt
     9301 cataaccatc cacgccccag cattaccatc aacaatccac aaattcaata aagatgtttt
     9361 tgctaagatg aaaaatcgaa gctacttaat caacgctgct agaggcccaa tcgtttctga
     9421 agaagcactt attgaagcac tgaaagaagg tgaaatcgcg ggtgcgggat tagatgtttt
     9481 tgagaatgaa ccacaggtat ctgaaggatt gagaagcctt gataatgtta tcatgagtcc
     9541 acatgctggg acaggtacta ttgagggacg tagaactttg gctgaagaag cagctgacaa
     9601 catcattgcc ttctttgatg gcaagccaca aaatattgtc aataaataat tggctagtga
     9661 gcgcagagcc atcttttgaa aaaatgggac ctcccatttt tttacgggta aatgtaagcg
     9721 cttaataatt taataaaggt gaacttatgc tagaagcttt acataaattc tttgaagtct
     9781 ttggtgcagc aactgttgta cccgctatga tttttattgt cagccttttt ctaaaggtca
     9841 atccaaggcg agctttcttt agcgccttac gagctggtgt tggactcaca ggctttggtt
     9901 ggattatatc agcttttgct cctatggtga ctaagtatat ccagcagatg gttaaaacta
     9961 ctggtctcca tttaccaatt gtagatattg gttggcaggc tggttctctc accgcctttt
    10021 cttctgagat tggcctctct ttctttgttt ttggcttgct aattgaattg gggcttttct
    10081 tgcttgggat tacccgagta tttgtcccat ctaatctttg gaataacttt ggctatatga
    10141 tttggggtac aatggcctat gcagcaacag gtaattttat attgtctttc gcatttatgg
    10201 tttttgtctt actctattca ttagtgatgt cagaagtatt ggcagatcga tggtcggagt
    10261 attacggtgt taaaaatgca acgatcaact cgattcataa tattgaaacc cttattccag
    10321 ctcttattct tgatcctctt tggaacctac ttggtgtcaa taaggtaaag ttgaacccag
    10381 aatcattaaa gactaagtta gggatatttg gtgagccaat gacacttggt tttatacttg
    10441 gtgtgattat tggtgtatta ggtagtttac gtaaccttgc aagtattgat acttggggag
    10501 gtatacttgg ttttgcagtt gctcttgcag cggttatgac tatttttcct ttgattacgg
    10561 gggtatttgc tagtgctttt gctcctcttg ctgaagcagt tgaacgcaat aagaaaaaag
    10621 aaagtcaagc tgagcaagga gcactggata aaaaacgttg gtttattgca gttgatgacg
    10681 gtgttggttt tggtgaacca gcaactatta tcgcaggtct cattttagta ccaataatgg
    10741 ttgttatttc cttgatttta cctggaaatg aagctttacc agtcgttgac cttattgcta
    10801 tcccttttat gattgaagct atgatagctg tgtcaaaagg aaatattctt aaagctattt
    10861 tgaatggaat catatggttt tcactaggtc tttatgctgc tagtgcccta ggtccaatct
    10921 acacagaagc tgttaagcat tttggaactg ctttaccagc aggggtaact ttgattatga
    10981 gctttaatct cctagctcat cctttgacag ctctagtctt ctttgcttgg atatctggga
    11041 acccactcta tattggtgtg acagttgtta tctatcttgc tttcttgttc ttgcttcgta
    11101 ctcatcgtga atctatttac gcatacctta aaactatggc tgataagaat agcggatatg
    11161 aaggtagttg tgaaaaaatc tttattgctg aagattaaaa atctgttgga ggaatactca
    11221 tgaaaaaaat tgatggacat gctcatattg ttgatacaat tgcaggattt aatggtaaag
    11281 gacgtcttaa tgcccttgga aacggttatg ctatttggga tgatggtgat ttaattaagc
    11341 tcatcccaga cgaatacggt gacaaagcat ttacagctga agcttttttg aagtatatgg
    11401 atgctaatgg tgtagataaa gcagtcattt tgcaaggtca tcttaatggt tatcaaaatt
    11461 attatacgca tttagctatt aaacgttatc cagaacgttt tacaggcgca ttctctgttg
    11521 atccatttgc agataacgca atgcaaattg taaaacgtca tgttgaagtc cttggttttc
    11581 gagctattaa atttgaaatt agtcaaggag ggggtatcca tggataccgt ggacaaaaaa
    11641 cgcctttccg tcttgataca gacccacatg tgagtcgtat tttgacttat ctgttagact
    11701 atccaggatt tgtagttaca gttgattatg gcaattggga tcaaattagt caccaacctg
    11761 atgctattgc taatttagct cgcctttata cgagtctcga ttttgttgtt tgtcacctct
    11821 cttttccaca tgttgagcat tcaaatcgat tgcgtgctga acttaatatg tggaaaaact
    11881 tcaacaatat ctatactgat atttcagcta ttcaagatat tgatgcccca gatagttttc
    11941 cttttccaaa atcagaacaa aatgtacgta ttgctaaaga agttcttggg gctaagcgta
    12001 tcatttgggg aactgattct ccatggtcag cgaccttcaa tacttacgaa gaattagcta
    12061 cttggctaga gaatgttgat attttcagtc aagaggaact agaagatgtt atgtacaata
    12121 atgctgagcg tgtttacttt aaagaagaac atgtaaaggc taacagagca ggtatagatg
    12181 atacaaccaa agagctcaat ctctattaga agagttttca aggtacaaga gtttcttgta
    12241 cctccttttt aaatcaacaa tgaggagaca aattatgact tattacctta gtattgacta
    12301 cggcggtaca aataccaagg cgcttatttt tgacaaatta ggacaccaaa tcgctgtttc
    12361 gagttttgaa actttaaaaa atgagactca atctggtcat cgtcaagtaa accttgttaa
    12421 aacgtggaat gctataactt ctgctattag agaggttatt caaatctcaa aactcagccc
    12481 tgagcagatt agtgcagtag catgtattgg acatgggaaa ggtctttatc tgctagataa
    12541 taagttggag ccacttgaac aaggaatttt gtctacagat aatcgtgcca aagatttggc
    12601 gcaatatttc gaatctaaac ttgataatat ttgggagttg actcggcaac atattttccc
    12661 ttcacaaagt ccagttattt tacgttggct taaagattat cagcccgaaa cctataaatc
    12721 aataggggcg gtcctttctg caaaggactt tattcggtat aagcttacag gaaaagtaca
    12781 gcaagaatat ggtgacgctt caggtaatca ttggataaat ttccaaacag gaacttatga
    12841 tccagctatt ttagattttt ttggcattag agagatagaa aactcacttc ctgaacttat
    12901 agatagtgca gatttagttc ctgggggaat tagttctcaa gcagcaaaag agactggtct
    12961 tgtagaaggg acccccgttg ttggagggct ctttgacatc gatgcttgtg ctcttggatc
    13021 aggtgtttta gagtcagata cttttagtgt tatttcggga acttggaata ttaatacata
    13081 tccaagttta aaaccagcaa agcaagatag tggtcttatg acttcctatt ttccagatcg
    13141 tcgttatctc ttagaggcaa gtagccctac ttctgcaggg aatcttaatt ttatgttaaa
    13201 aatgctcatg catcaagaaa ttgataacgc taaatctagt ggaggttcta tctatgataa
    13261 tttagaagaa tttctcactc atactgatgc tacacatcat ggacttattt tctttccgtt
    13321 tctttacggt agtaacacat cacaagatgc tagcgcttgc ttttttgggc taacaactaa
    13381 atcgacgaaa tctcagatga tacgtgcggt atatgaaggt attgcgtttg cacataagca
    13441 gcatatcact gatttaataa aaagtagggg cagtgtgcca aaaataattc gtttctctgg
    13501 cggagctacc aactcaccag catggatgca aatgttttct gatatcttaa actttcctat
    13561 tgaaacagta gaaggcacag aattaggagg gttaggagga gctattttag cacgtcatgc
    13621 tttagataag atttcgttaa aggaagcagt ccaagatatg gttcgtgtaa aagctattta
    13681 taaacctcaa ttatccgaag taaaggggta caaaaaaaaa tatcacgctt accaaaaatt
    13741 attagaaaca ctggatccta tttggtcgga actcggtcat ctgaataagt agggatatca
    13801 aaggagttgt tatgaaacaa gaagatatta ttatcaatag tattgtattc aaaaaacagt
    13861 tagacaaagg ttttacacaa gttcagctca ttaagttagt aaaggattta ggattttcac
    13921 gatttgagat acgtcaagaa ttgcttcaag atccagatag agagctaccc gctttaaaag
    13981 cagaggctga cttttatgat atcaatcttt actatagtgc taatgaagac cttatcaaag
    14041 gagggaaagt taatccttat cttaataaag gccttaaaga agcatcacag ttaggagctc
    14101 ctttcattaa gctaaatgtt ggacaaacca gaaacttaag taaagaagag ttagaacctc
    14161 ttaaagagat attgaaaagc caaactattg gtataaaggt ggaaaataat caagacccta
    14221 aagcagctac agtggagaat tgccaatatt ttatgactct agtgaaagaa ttacaaatac
    14281 caataagttt tgtttttgat actgctaatt gggcctttat caaccaagat ctataccaag
    14341 cggtaaatag cttagcttgt gataccacct accttcactg taaaaatttt ttacaggtag
    14401 ctggaaagcc tcacctctcg aagagcctct tcgaaggaga gattgatctg actgatttgt
    14461 taaagagttt ctctaattgt gagtatctag ctctagaata ccccacagaa ttggaggtat
    14521 taaaaaggga tgttcaacgt ttaatctcta tcagcaatag tcaaaacttt taatgaaaag
    14581 gctttaaaaa gatgttataa taagtctgta tgaattcgag tagaaaagag gtatattctt
    14641 gattattctc gataaaaaaa gttatgacct cctcttttac ctattgaaat tagaggaacc
    14701 tgaaacagtt atggcaattg ccaacgcact taatcagtct aggcgtaaag tgtattatca
    14761 cttagagaag ataaacgatg cactgcctag cgatgtgcct cagattgtta gttatccacg
    14821 tgtaggaatc ttgctaaccg aaaaacaaaa agcagcctgt cgtcttttat tagatgaagt
    14881 aactgattat agttacgtca tgaaaagtag tgagaggttg cagctgtctt tagtatctat
    14941 cgtggtagct aaggaccgca taacgattga taggttgatg caactaaacg atgtttctcg
    15001 aaatactatc ttaaacgatt taaacgaatt aagaagtgag ttagcagaga aagaatataa
    15061 tttacagtta caatcaacaa aatgtcgcgg ttatttttta gatggtcacc cattgtccat
    15121 tatccagtac ttatataagc tcttagatga tatctaccat aatggaagta gtagttttat
    15181 agaccttttt aatcataaac tgtctcaagc ttttggtgcc agcacatatt tttctaaaga
    15241 ggttcttgat tattttcacc attatctctt catttctcaa cgaagtctag gtaagaaaat
    15301 caacagtcaa gatggtcagt ttatgattca gattttgcct tttatactaa tggcttatcg
    15361 taagatgcga ttaagtcctg aagtacagac ctctcttaat agtgatttta gcttggtttg
    15421 gcaacgtaag gaatatgaga ttgctaaaga gttggctgat gagctggaag aaaattttca
    15481 gttatcactg gatgagattg aagtgggact agtggccatg cttatgctta gtttccgcaa
    15541 ggaccgtgac aatcatttag agagccagga ttatgatgat atgcgagcta ctctaaccag
    15601 ttttttgaaa gaattggaag aacgatatca ccttcacttt gttcataaaa aggacttact
    15661 aagacaactt ctaactcact gcaaggcact cttatatcgt aaacgttatg gtattttttc
    15721 tgttaatcct ttaacagagc atattaaaga caaatatgaa gaactttttg ccataacctc
    15781 gtcttctgta aagcttttag agaaagcttg gcaaatcaaa ttgaccgatg atgatgtagc
    15841 atatctaacg attcatttag gaggggaact tcgtaatagt caacaatctc ctaataaact
    15901 taagttagtt attgtatctg atgaaggaat agcgattcag aaacttcttt taaagcaatg
    15961 tcaacgctac ttaacaaaca gtgatataga agctgttttt acaaccgaac agtaccaaag
    16021 tgtgagtgat cttatgcatg tagatatggt tgtctctact agtgacgctt tagaatctcg
    16081 ttttccgatg ttagtagttc accctgtttt gacagatgat gatattattc gcttgattcg
    16141 cttttcaaaa aaaggtaact gtgcaaatag taatcaattt accaatgaac ttgaaaaaac
    16201 gattgctcaa tatgtcaagg aagatagtga acgctacgtg ctgaaatcta agattgagaa
    16261 acttattcat caagaattgc tccaagacgt ccttcccctt caaagtacag tttgttaaaa
    16321 tataatttta tggtagcctc tcagcttttc tgaagacaga aaaagaagct ggggcttttt
    16381 tggtgctttt aaacaatgtg ctagatattt aatcgcaatg agttcctcta tgacatgata
    16441 attaaagttg agaaagctag tacttttaat gggagtactt tttatttgtg cttattttaa
    16501 ttaaaattat gagtctaatt tggaacagac agcccagttt tgaacacata aatgtgtgac
    16561 acttggtctt tttttcaaaa gtatttgaac ttatactagt agatgtaaga aacaattaat
    16621 caggaggaaa taaaatgcct aacgttaaag atattactag agaatcatgg attttatcaa
    16681 cattcccaga atggggaaca tggctaaacg aagaaattga agaagaagtt gttgctgatg
    16741 gcaactttgc aatgtggtgg ttaggaaact gtggtgtttg gattaaaaca ccaggtggtg
    16801 ctaatgttgt tatggaccta tggtcaaacc gcggtaaatc aacaaaaaaa gtaaaagata
    16861 tggttcgtgg acaccaaatg gctaatatgg caggtgttcg taaactccaa ccaaacttac
    16921 gcgcacaacc aatggttatt gatccatttg ctattaatga attggattac tatcttgttt
    16981 cacatttcca cagtgaccac attgatatta acactgcggc agccattatt aataatccta
    17041 acttagacca tgttaaattt gttgggccat atgaatgtgg tgaaatttgg aaaaaatggg
    17101 gagttccaga agaacgaatc atagtgatta aaccaggtga atcatttgag tttaaagaca
    17161 tcaaagttac tgctgttgaa tcatttgacc gtacttgctt ggtgacactt ccagtagatg
    17221 gtgcagaaga acatgatgga gaacttgctg gtttagccgt gactgatgaa gaaatggctc
    17281 gtaaagcagt taactatatt tttgaaacac caggtggaac aatctaccat ggagctgact
    17341 cacatttttc aaattatttt gctaaacatg gtaaagacta caagattgac gtagctatta
    17401 acaactatgg tgataaccca gttggtattc aggataaaat gacttcaatc gatcttcttc
    17461 gtatggcaga aaatctccgt gccaaagtca ttattcctgt acactacgat atctggtcta
    17521 acttcatggc ttctacagac gaaatcttac aattgtggaa aatgcgtaaa gaacgccttc
    17581 aatatgactt ccatccattt atttgggaag ttggaggtaa atacacttat ccacaagata
    17641 aagatagaat tgaataccat cacccacgtg gttttgatga ttgcttcgaa caagaatcaa
    17701 atattcaatt taaagcactt ctataatggc taatgagaga ctgtatgtcg catgatatgc
    17761 agtttttcaa ttttaaaata tgttactaaa atcacaatta agattaggag aaataatgtc
    17821 agaatttgat acaaaatcat atttagaaaa acttgatgca tggtggagag cagctaatta
    17881 tatttctgca gcacaaatgt atctaaagga taatcctctc ttgagacgag agctagttga
    17941 aaacgactta aaggttcatc caattggtca ctggggcact gtacctggac aaaactttat
    18001 ctatgctcac ttaaatcgcg ctattaataa atacgattta gacatgttct atattgaagg
    18061 tccaggtcat ggtggtcaag ttatggtatc taattcatat ttagatggtt catatactga
    18121 attaaaccca aatattgagc aaacagagga tggctttaag cagttatgta aaatcttctc
    18181 tttcccaggt ggaattgcat cccatgcagc accagaaaca ccagggtcaa ttcatgaagg
    18241 tggtgaactt ggttacgcac tttctcatgc gacaggtgcc atcctagaca atccagatgt
    18301 tattgctgcg actgttattg gtgatggtga aggtgaaaca ggaccactta tggctggttg
    18361 gctatctaat acctttatca atccagtaaa tgatggtgct gttctaccaa tcttctattt
    18421 aaatggtggt aaaatccata acccaactat ctttgaacgt aagacagatg aagaattatc
    18481 tcagtttttt gaaggattag gttggaaacc tatttttgca gatgttgttg aactctctga
    18541 ggatcatgcg gctgctcatg ctttgtttgc agaaaaatta gatcaagcta ttcaagagat
    18601 taaaaccatt caatcagaag cacgacaaaa accagcagaa gaagctatcc aagcaaaatt
    18661 ccctgtcttg gttgcacgta ttcctaaggg gtggactggt ccaaaagctt gggaaggaac
    18721 accaattgaa ggtggcttcc gcgctcacca agtaccaatt ccagtagatg cccaccatat
    18781 ggaacatgtc gattctcttt tgtcatggct tcaatcatac cgtccagaag aattatttga
    18841 tgaaagtggt aaaatcgttg atgagattgc tgctatttca ccaaaaggtg atcgtcgcat
    18901 gtccatgaat ccaataacca atgctggtat tgttaaagca atggatacag cagattggaa
    18961 gaaatttgct cttgatatta atgttccagg tcaaattatg gcacaagata tgattgaatt
    19021 tggaaaatat gcagcagatt tggtagatgc taatccagat aatttccgta tttttggtcc
    19081 agatgaaacg aaatcaaatc gtcttcaaga agtgtttaca cgtactagcc gtcaatggct
    19141 tggtcgtcgt aaaccagatt atgacgaagc tttaagtcca gctggacgtg ttattgattc
    19201 ccaattgtct gaacatcaag cagaaggttt cttagaaggt tatgttttaa ctggtcgtca
    19261 cggcttcttt gcttcatacg aatcattcct tcgcgttgta gattcaatgg taacacaaca
    19321 tttcaaatgg ttacgtaaat caaaaacaca cacaacatgg cgtaaaaatt atccagcgct
    19381 taacttaatt gcagcttcaa ctgttttcca acaagatcat aatggttaca ctcaccaaga
    19441 tccaggtatc ttaactcact tagctgaaaa aacacctgaa tacattcgtg aatatttacc
    19501 agcagatact aactcacttc tagctgttat ggataaagcg tttaaagctg aagataaaat
    19561 taatttaatt gtgacatcta agcaccctcg tccacaattt tactctattg ctgaagcaga
    19621 agagttagtg gcagaaggat ataaggtgat tgactgggct tcaaatgtgt cgcttaatca
    19681 agagccagat gttgtctttg ctgcggcggg aacagaacct aacttagaag ctttggcagc
    19741 tattagtatt cttcataagg ctttcccaga acttaagatt agatttgtta atgtattaga
    19801 catcctaaaa cttcgtcatc cttcacaaga tgctcgcggt ttatcagatg aagagtttga
    19861 caaagtcttt accacagata aacctgttat ttttgctttc catagttatg aagatatgat
    19921 tcgagatatt ttctttagtc gtcacaatca taatttgcat acgcatggtt accgtgaaaa
    19981 tggtgatatc acaacaccat ttgatatgcg cgtgatgtca gaattggacc gattccatct
    20041 ggcacaggat gcagctcttg cttctttagg aaatgaagca caagccttca gtgatgaaat
    20101 gaatcaaatg gttgcttatc acaaagacta tattcgtgaa cacggagacg atattccaga
    20161 agtgcaaaat tggaaatggg aaaatatcaa gtagtcactt ttttcaggcc tatacttaca
    20221 aacaaaaaca cttttagatt ttttctaagg gtgttttgtt attttataga agaatgaccg
    20281 ctttttcaaa agcgttaagt ggtcactttt aaagttgtaa tattaatact aataagtctt
    20341 taaataattc aataatgcca taacaataat ggtgtgggct aattaaaata tctctaagca
    20401 aaggtgttga cataaagaga ccatacatga tattatagtt atgttctgtt tcggcagttt
    20461 atttttttga gtaaaaaagt gactaccctt ttacgatact ttaacgtcac tttatagata
    20521 agacacataa ccttaaaaat aagatacgaa atttttagta ggagataaga atgacaaata
    20581 ttttagaagt gaagaattta accaaaattt ttgggaaaaa gcaaaaagct gctcttgaaa
    20641 tggttaagca aggaaaaagt aagacagaaa ttcttgaaaa aacaggagcg actgtaggtg
    20701 tctatgatgc gagttttgaa ataaaagaag gcgaaatttt cgttatcatg ggactttctg
    20761 gttcaggaaa atcaacttta gttcggatgt taaaccgttt gattgaccca tcatcgggaa
    20821 atatctattt agatggtaaa gatattgcaa agatgaatgt agaagattta cgtaacattc
    20881 gtcgtcacga tatcaacatg gttttccaaa attttggtct tttcccacat cgtacaattt
    20941 tagaaaatac agagtttggg ttagagatgc gtggtgtttc taaggaagaa cgtacaatgt
    21001 tagctgaaaa agctttggac aatgcaggtc tattaccgtt taaagatcaa taccccagtc
    21061 aattatcagg aggaatgcaa cagcgtgttg gtttagcacg tgcccttgct aacagcccta
    21121 aaatccttct gatggatgag gccttctcag ctttagaccc attaattcgt cgtgaaatgc
    21181 aagatgagct tcttgatctt caagacacga ataaacaaac tattattttt atcagccatg
    21241 atttgaatga agcgttacgt attggtgatc gtattgctct gatgaaagat ggggaaatca
    21301 tgcagattgg tacaggagaa gagattttga ctaatccggc taatgatttt gttcgtgagt
    21361 ttgttgaaga cgttgatcgc tcaaaagtgt taactgctca aaatattatg ataaaacctt
    21421 taacaactgt tttagaaatt gatggcccac aagttgcttt aacaagaatg catcgcgagg
    21481 aagtctcaat gttaatggca acgaatcgtc gtcgccaact tctgggatcc ctaactgcag
    21541 atgctgcaat tgaagctcgt aagaaaggtt tgccgctttc tgaagtgatt gataaagatg
    21601 ttgtaacggt ctcaaaagat acagttatta cagatatcat gcctttaatt tatgattcat
    21661 ctgctccgat agccgttact gatgataatg atagactcct aggggttatc attagaggac
    21721 gtgtgatcga agcacttgct aacgttcaag atgaaacagt agtagaatcg ccaaaagaaa
    21781 cggtagaagc ttaatagaga ggacaaatca ttttggaaaa tttattacaa cataagttac
    21841 cggtcgcacc atttgtagag tcaacgacca attggattac gaaaactttt tctggtttat
    21901 ttgactttat ccagacaatc ggtaatgcat tgatggattg gatgacaaaa acattactat
    21961 tcattaatcc tttacttttt attgtactaa ttacagttgc tgtatttttc ttagctaaga
    22021 aaaaatggca attaccgaca tttactttca ttggtttgct atttatctat aaccaagggc
    22081 tgtgggagca gttgattaat acttttaact tggttttagt agcaagttta atttctatta
    22141 ttattggtgt accactaggt atttggatgg caaaaagtga taaagttaaa caagttgtta
    22201 atccaattct tgactttatg caaactatgc cagcattcgt ttaccttatt cctgcagtag
    22261 ccttctttgg tatcggtatg gtaccaggtg tctttgcctc tgttgtattt gctttgcctc
    22321 caacagtgcg ctttactaac ttggctattc gtgaaatccc acttgaatta atagaagcat
    22381 ctgattcatt tggttcaaca gctaagcaaa aattattcaa agttgaacta ccacttgcta
    22441 agaacactat tatggcagga atcaatcaaa ctatgatgtt ggctctttct atggtggtta
    22501 caggatctat gatcggtgca cctggacttg gtcgtgaggt gttatcggcg cttcaacatg
    22561 ctgatattgg tacaggattt gttagtggtc tatcgctagt tattttagca atcgtgttag
    22621 accgtgttag tcaattcttt aattccaaac cgggtgagaa gcaagctaaa acatcaaaag
    22681 ttaaaaaatg ggtaggttta ggggccttgg ttctcttcat tctagcagct cttggtcgca
    22741 ttgttgtcaa tatgacttca ggaaatgaag ccaaaggtca aaaagtgaaa atagcttatg
    22801 tccaatggga ttcagaagtt gcttcaacca acgttattgc tgaagtttta aaatctaaag
    22861 gatatgatgt tgaattgaca ccacttgata atgcggtcat gtggcaaaca gtcgccaatg
    22921 ggaatgctga ttttacaaca agtgcttggt tgccaaagac ccacgggcaa tattttaata
    22981 aatacaaaaa tagtttagat gacttaggtc ctcacgttga gaacgtcaaa ataggtttag
    23041 ttgttcctaa atacatgaac gtaaatagta ttgaagaatt atcaaatcaa gcagataaac
    23101 aaattacagg tatcgaacca ggtgcaggta ttatgaagtc tgctaaacaa tcccttaaag
    23161 attatccaaa cctttcaagt tggaagttgt tatcagcatc aacaggagct atgacaacaa
    23221 ccttagggaa ggctatcaaa aacaaagatc aagtagtcat tacaggttgg tcacctcact
    23281 ggatgtttgc taagtatgat ttaaaatacc ttaaagatcc taagaaatca tttggtggtg
    23341 aagaacatat caatacaata gcacgcaaga atttgaaaaa agatatgcca aaagtatata
    23401 aaattatcga taagttcaag tggactaaag aagatatgga atctatcatg ttagatatgg
    23461 ataaaggtat ggagcctgct aaagcagctc aaaaatggat taaaaaccac aaaaaagaag
    23521 tttcagagtg ga
//
LOCUS       DATRTN010000020        22108 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.020, whole genome shotgun sequence.
ACCESSION   DATRTN010000020 DATRTN010000000
VERSION     DATRTN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22108
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.020"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            105..587
                     /locus_tag="VBE22_000852"
     CDS             105..587
                     /locus_tag="VBE22_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202323.1"
                     /translation="MKNEYEEILKKDNLFEHEHAEFKESLNLNESFYLQLKKEEMKPS
                     DNLKKEEKPRENSVKERETPAENNDFVSVTEKNNLIDKYKELLSKIPENTQNPGEKNI
                     RNYLEKEYEELLQKDKLFKHEYTEFTKSLNLNETFYSQLKEGEMKLSENPEKGETNTN
                     "
     gene            complement(758..1372)
                     /locus_tag="VBE22_000853"
     CDS             complement(758..1372)
                     /locus_tag="VBE22_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO7202324.1"
                     /translation="MSFLEELKNRRSIYALGRNTEVSDEKIVEIIKEAVRQSPSAFNS
                     QTSRVVILLNDEVTKFWDELVANDLVETMKVQGAPETAIAGTKEKLASFGASKGTVLF
                     FEDQDVVKSLQEQFVLYADNFPVWSEQSTGIASVNTWTALSAELGLGGNLQHYNPVID
                     ASVQAVYGVPASWKLRGQLNFGSIEAETGEKEFMNDDDRFKVIG"
     gene            complement(1505..1930)
                     /locus_tag="VBE22_000854"
     CDS             complement(1505..1930)
                     /locus_tag="VBE22_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7202325.1"
                     /translation="MGNFKNSAVKSMVVMRKAFRTIDGKVSESFKEFELTPTQFAVLD
                     VLYAKGTMKIGELIENMLATSGNMTVVIKNMEKKGWVLRHSCPNDKRAFLVSLTTEGE
                     EVIKKALPEHIKRVEDAFSVLTETEQEDLINLLKKFKTL"
     gene            complement(2057..6463)
                     /locus_tag="VBE22_000855"
     CDS             complement(2057..6463)
                     /locus_tag="VBE22_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO7202326.1"
                     /translation="MSELFKKLMDQIEMPLEIKNSSVFSSADIIEVKVHSLSRLWEFH
                     FSFPELLPIEVYRELQTRLVNSFEKADIKATFDIRAETIDFSDDLLQDYYQQAFCEPL
                     CNSASFKSSFSQLKVHYNGSQMIISAPQFVNNNHFRQNHLPRLEQQFSLFGFGKLAID
                     MVSDEQMTQDLKSSFETNREQLLEKANQEAMQALEAQKSLEDSAPPSEEVTPTQNYDF
                     KERIKQRQAGFEKAEITPMIEVTTEENRIVFEGMVFSVERKTTRTGRHIINFKMTDYT
                     SSFAMQKWAKDDEELKKYDMISKGSWLRVRGNIENNNFTKSLTMNVQDIKEIVHHERK
                     DLMPADQKRVEFHAHTNMSTMDALPTVESLIDTAAKWGHPAIAITDHANVQSFPHGYH
                     RAKKAGIKAIFGLEANIVEDKVPISYNEVDMNLHEATYVVFDVETTGLSAANNDLIQI
                     AASKMFKGNIIEQFDEFIDPGHPLSAFTTELTGITDNHVRGSKPILQVLQEFQNFCQG
                     TVLVAHNATFDVGFMNANYERHNLPLITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQ
                     VALEHHHMANYDAEATGRLLFIFLKEARENRDVTNLMELNTKLVAEDSYKKARIKHAT
                     IYVQNQVGLKNIFKLVSLSNVKYFEGVARIPRSVLDAHREGLLLGTACSDGEVFDALL
                     SNGIDAAVTLAKYYDFIEVMPPAIYRPLVVRDLIKDEVGIQQIIRDLIEVGRRLDKPV
                     LATGNVHYIEPEDEIYREIIVRSLGQGAMINRTIGRGEDAQPAPLPKAHFRTTNEMLD
                     EFAFLGKDLAYEIVVTNTNTFADRFEDVEVVKGDLYTPFVDRAEERVAELTYAKAFEI
                     YGNPLPDIIDLRIEKELASILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVA
                     TMIGITEVNPMPPHYVCPNCQHSEFITDGSCGSGYDLPNKNCPKCGTLYKKDGQDIPF
                     ETFLGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEEYAFRAGTVGTVAEKTAFGFVK
                     GYERDYNKFYNDAEVERLATGAAGVKRSTGQHPGGIVVIPNYMDVYDFTPVQYPADDM
                     TAAWQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPSNILPDDPDVMKLFS
                     GTEVLGVTEEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWL
                     GNAQDLIKEGIATLSTVIGCRDDIMVYLMHAGLQPKMAFTIMERVRKGLWLKISEDER
                     NGYIQAMRDNNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHYPIFYYCAYF
                     SIRAKAFELRTMSAGLDAVKARMKDITEKRQRNEATNVENDLFTTLELVNEMLERGFK
                     FGKLDLYRSHATDFIIEEDTLIPPFVAMEGLGENVAKQIVRAREDGEFLSKTELRKRG
                     GVSSTLVEKFDEMGILGNLPEDNQLSLFDDFF"
     gene            complement(6586..7170)
                     /locus_tag="VBE22_000856"
     CDS             complement(6586..7170)
                     /locus_tag="VBE22_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEO7202327.1"
                     /translation="MKSRKKDKLVLRLTTTLLVFGLGGVWFYNYKNDNVEPTVTSASD
                     QTTTFIQTISPTAIEISKTYDLYASVLLAQAILESSSGQSDLSKAPNYNLFGIKGEYK
                     GKSVQMPTLEDDGKGNMTQIQAPFRAYPNYSASLYDYAELVSSQKYASVWKSNTSSYK
                     DATAALTGLYATDTAYASKLNQIIETYSLDAYDK"
     gene            complement(7295..9148)
                     /locus_tag="VBE22_000857"
     CDS             complement(7295..9148)
                     /locus_tag="VBE22_000857"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEO7202328.1"
                     /translation="MKQSKMLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMRQEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDQS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHKDYEDLDVTYEDYRKAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPNRT
                     DLNRWLVLDKTIPSIDDIPEDVLEEIKVELSAWLVSGEDTIAYSTESSYAANLEMATN
                     EYKPSTKAATFEEVTKVETPNCKSIDEVAGFLSIDENQTIKTLLFIADEQPVVALLVG
                     NDQVNDVKLKNYLAADFLEPASEEQAKEIFGAGFGSLGPVNLPDSVKIIADRKVQDLA
                     NAVSGANQDGYHFTGVNPERDFTAEYVDIREVKEGEISPDGKGTLKFARGIEIGHIFK
                     LGTRYSDSMGANILDENGRSNPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGAYRF
                     AWGINFPEELAPFDVHLITVNVKDQESQDLTEKIEADLMLKGYEVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKASEGIVEVKIKASGDTIEVHADNLIETLEILTKK"
     gene            complement(9240..10499)
                     /gene="rseP"
                     /locus_tag="VBE22_000858"
     CDS             complement(9240..10499)
                     /gene="rseP"
                     /locus_tag="VBE22_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEO7202329.1"
                     /translation="MLGILTFIIIFGVIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHIDKEGTTYTIRILPLGGYVRMAGWGDDKTEIKTGTPASLTLNKEGIVTRINLSGK
                     QLDNTSLPINVTAYDLEDKLTITGLVLSETKTYSVDHDATIIEEDGTEIRIAPLDMQY
                     QNASVWGRLITNFAGPMNNFILGLVVFIALAFIQGGVQDLSTNQVRVSENGPAASAGL
                     KNNDRILQIGSHKVSNWEQLTAAVEKSTRHLEKKQKLALKIKSKEVVKTINVKPQKVD
                     KSYIIGIMPALKTSFKDKLLGGLKLAWESFFRILNELKKLIAHFSINKLGGPVALYQA
                     SSQAAKNGFVTVLNLMGLISINLGIMNLIPIPALDGGKIVMNILEAIRRKPLKQETET
                     YITLAGVAVMLVLMIAVTWNDIMRAFF"
     gene            complement(10530..11324)
                     /locus_tag="VBE22_000859"
     CDS             complement(10530..11324)
                     /locus_tag="VBE22_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEO7202330.1"
                     /translation="MKERVIWGAVALAIFIPFLVMGGLPFQFLVGLLAMIGVSELLRM
                     RRLEIFSFEGALAMIGAFVLTVPLDSYLSFLPVDASLSAYGIVIFMILAGTVLNSNSY
                     SFEDAAFPIASSFYVGIGFQNLVSARMAGIDKVLLALFIVWATDIGAYMIGRQFGQRK
                     LLPSVSPNKTIEGSLGGIASAIVVAFFFMLFDKTVYAPHSFLVMLVLVAIFSIFGQFG
                     DLVESSIKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHFFGLF"
     gene            complement(11339..12091)
                     /locus_tag="VBE22_000860"
     CDS             complement(11339..12091)
                     /locus_tag="VBE22_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEO7202331.1"
                     /translation="MFIFKRKKQVEMPLEKIPAHIGIIMDGNGRWAKKRLKPRVMGHK
                     AGMDALQEVTIAASGLGVKVLTVYAFSTENWSRPDDEVKFIMNLPVEFFDKYVPELDK
                     NNVRVQVIGDTHKLPKATYDAMQRACLRTKHNSGLVLNFALNYGGRSEITNAIKEIAQ
                     DVLEAKLNPDDITEDLVANHLMTNSLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEF
                     YFTPVLWPDFKKDELHKAIVDYNQRHRRFGSV"
     gene            complement(12258..12599)
                     /gene="yajC"
                     /locus_tag="VBE22_000861"
     CDS             complement(12258..12599)
                     /gene="yajC"
                     /locus_tag="VBE22_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEO7202332.1"
                     /translation="MSLPIIMLVVMVGMMFFMQRQQKKQAQERQKQLNAVQKGDEIVT
                     IGGLFGVVDEVNTEAQRMVLDVDGVYLTFELAAIKSVVSKAATPTEPVEDVKIIEVNE
                     DENKETAIETE"
     gene            complement(12676..13020)
                     /locus_tag="VBE22_000862"
     CDS             complement(12676..13020)
                     /locus_tag="VBE22_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEO7202333.1"
                     /translation="MYSFEELLATMTLITAAEIEDKIDSNQDFVLFIGRISCPFCHLF
                     VPKIVEVADEDEFELFHLDSEDFDHWTANKEFRNKYDIPTVPGLMVVKNGTIKVKCDS
                     KMTKEEIREFIG"
     gene            complement(13211..14374)
                     /locus_tag="VBE22_000863"
     CDS             complement(13211..14374)
                     /locus_tag="VBE22_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEO7202334.1"
                     /translation="MSENLGQLAINQARENGGKLEVISKVKVEDKRDLSIAYTPGVAS
                     VSSAIAEDVELAYELTTKKNTVAVVSDGSAVLGLGDIGPEAAMPVMEGKAALFKRFAN
                     VDAVPIVLKTNDTEEIISIVKAISPTFGGINLEDISAPRCFEIEQRLIEECDIPVFHD
                     DQHGTAIVVLAALFNSLKLVKKDIEDIRVVVNGGGSAGLSITRKLLSAGAKHVTVVDR
                     FGIINDKDRESLAPHHKAIAKLTNREFQSGSLEDALENADVFIGVSAPEALHAEWISK
                     MADKPIVFAMANPIPEIYPDQALKAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAARGIASLIPEEELSTTHIIPNAFQNDVADVVAKSVSNAVQK"
     gene            complement(14399..15736)
                     /locus_tag="VBE22_000864"
     CDS             complement(14399..15736)
                     /locus_tag="VBE22_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEO7202335.1"
                     /translation="MADVKVVNNEDSRGQKQDLKAKLFHIKIGSVPLPVYVCLALLIL
                     LAGFLQKLPVNMLGGFAVILTMGWFLGTIGASIPGFKNFGGPAILSLLVPSILVFFNL
                     INKNVLESTNMLMKQANFLYFYIACLVSGSILGMNRKMLIQGLLRMIFPMLLGMVCAM
                     MVGTFVGVILGLEWRHTLFYIVTPVLAGGIGEGILPLSLGYSSITGVASEQLVAQLIP
                     ATIIGNFFAILCTALLNRLGEKKPHLSGQGQLVRLNKGEDMSDIIADHSGPIDVKKMG
                     GGVLTACSLFIFGHLLQQLTGFPGPVLMIVAAAILKYINVIPRETQNGAKQLYKFISG
                     NFTFPLMAGLGLLYIPLKDVVATLSIQYFIVVISVVFTVISVGFFVSRFLNMNPVEAG
                     IISACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRMLF"
     gene            15890..17416
                     /locus_tag="VBE22_000865"
     CDS             15890..17416
                     /locus_tag="VBE22_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7202336.1"
                     /translation="MKKKLSLWAFLSLILVTMTICIFSIFYYVTIHQSYRMVRVQEEK
                     ILKNTGYALSRNPQVIQTLKDNHYDQSLQKQMLFLSKKSNLDYIVLINLKGIRFTHPD
                     STKIGKPFQGGDEQAVFKGKAIMSTAEGSLGKSLRYLIPVYDHQKQVGAIAVGLKLTT
                     LGDLSQSSIKEFSKPLLISILISLVVTSIISYGLKKQLHNLHPSDIFQHLEERNATLD
                     QIQAAVFVIDQRHIIKRNNPAASLLFKKEGQRDLFSGKLLESLIPQLKQDHFSKKTEQ
                     VLHFQGQDYLLSISPITVKTQNRGYVVFLRNVTETLFTLDQLAHTTAYASALQAQTHQ
                     FMNQLHVIYGLADIEYYDELKIYLKELLEPQNEFLARLSMLVREPRLASFIIGEREKF
                     AEKHINLSTEILVEIPTKSTVEDVNNYLLLHRYINTKILTLLNSTTLVSLRLNYQNNL
                     IETDYQWENEKWLLNDYHQYFNDAYFQQLLVDSRATYKISLKQNKVIIRTNIIYSEGP
                     "
     gene            17418..18107
                     /locus_tag="VBE22_000866"
     CDS             17418..18107
                     /locus_tag="VBE22_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO7202337.1"
                     /translation="MDVLIIEDDPMVEFIHRNYLEKLNYFQNIYSTASQTQAIAYLND
                     IKIQLVLLDIHIKEGNGLELLKLLRNQHQNTEVIVISAANEAHTVKEAFHLGIVDYLI
                     KPFTFERFESSIEKFLNHYHTFEADKIYQDNIDHFQKIDSGWLEGEVKLDEKGLSEIT
                     YQHILDAIQELEQPFTIQELAKCSQFSHVSVRKYIAYMEEKGLLTSQQIYTKVGRPYK
                     VYKLIKKNLGL"
     gene            complement(18120..19115)
                     /gene="galE"
                     /locus_tag="VBE22_000867"
     CDS             complement(18120..19115)
                     /gene="galE"
                     /locus_tag="VBE22_000867"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEO7202338.1"
                     /translation="MAVLILGGAGYIGSHMVDQLITQGKEKVIVVDNLVTGHRQAVHS
                     DAIFYEGDLSDKTFMRQVFRENPDVDAVIHFAAFSLVAESMENPLKYFDNNTAGMIKL
                     LEVMNECDIKNIVFSSTAATYGIPEQVPILETAPQNPINPYGESKLMMETIMKWADQA
                     YGIKFVALRYFNVAGDKPDGSIGEDHKPETHLLPIILQVAQGVRDKIMIFGDDYNTPD
                     GTNVRDYVHPFDLADAHILAVDYLRQGNESNVFNLGSSTGFSNLQMLEAARRITGKEI
                     PAQKAARRPGDPDTLIASSEKARQILGWEPKFDNIDKIISSAWAWHSSHPNGYED"
     gene            complement(19203..20810)
                     /locus_tag="VBE22_000868"
     CDS             complement(19203..20810)
                     /locus_tag="VBE22_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEO7202339.1"
                     /translation="MKKHWWHKATIYQIYPRSFMDSDGDGVGDIKGITSKLDYLEKLG
                     ITAIWLSPVYQSPMDDNGYDISDYQAIADIFGDMNDMDQLLQEANQRGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDKVSGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIDKGIGGFRMDVIDLIGKIPDKGITGNGPKLHDYL
                     KEMNRASFGKHDLLTVGETWGATPDIAKQYSNPDNEELSMVFQFEHVGLQHKPDAPKW
                     DYSDGLDVPALKDIFTKWQTQLELGQGWNSLFWNNHDLPRVLSIWGNDSDNRKQSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFECLADVDDIESLNYAKEAMDNGVSEATILDS
                     IRKVGRDNARTPMQWSQEHQAGFTKGTPWLAVNPNYQEINVEAALNDTESIFYTYQKL
                     VALRKEHDWLVDADFKLLETADKVFAYVRQTDKERYLIVANLSDQNQSFEFPEAVKET
                     IISNTEVQEVLSSNTLKPWDAFCIELF"
     gene            complement(20938..22071)
                     /gene="ugpC"
                     /locus_tag="VBE22_000869"
     CDS             complement(20938..22071)
                     /gene="ugpC"
                     /locus_tag="VBE22_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEO7202340.1"
                     /translation="MVELNLNHIYKKYPSASHYSVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIDGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKFSKQEIDKRVREAAANIGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHQRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPDGD
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPSMNFFKVKVENGMIISEDGLRIAIPEG
                     QEKLLESRGYKGKELIFGIRPEDISSNLLVQDTYPNANVEAEVLVSELLGSETMLYVK
                     LGQTEFASRVEARDFHNPGEKVNLTFNVAKGHFFDADTEQAIR"
BASE COUNT         7210 a         4493 c         3466 g         6939 t
ORIGIN      
        1 taacagaaaa agaaaaaaac gagctgatag ataagtataa aaaattattg tcacagattc
       61 ctgaaaacac acaaaatcca ggagaaaaaa acataagaga ttatttgaaa aatgaatatg
      121 aagaaatatt aaagaaagat aatttatttg agcatgaaca tgcagaattt aaagaaagtc
      181 taaatttaaa tgaatcattc tacctacaat taaagaaaga agaaatgaaa ccgagtgata
      241 atctcaaaaa agaagaaaaa ccaagagaaa attcagtaaa agagagggag actccagctg
      301 aaaacaatga ttttgtttca gtaacagaaa aaaacaactt gattgataaa tataaagaat
      361 tattatcaaa aatcccagag aatacacaaa atcctgggga aaagaatata cgaaattatc
      421 tagagaaaga atatgaagaa ctactacaaa aagataaatt attcaagcat gagtacacag
      481 agtttacaaa aagtctaaat ttgaatgaaa cattctattc acaattaaaa gaaggagaaa
      541 tgaaactaag tgaaaatccc gaaaaaggag aaacgaacac gaattaacct ttcaaatatc
      601 tacatcaaat aacagaagtc tctgtatcaa gcgaattgta gcttttttga atatactaaa
      661 aacactccta cctctatttt actagaagta ggagtgtttt ttcattttag ttcatagatg
      721 cagtgtcatt gctatccttg cctttttttg tacagtatta tccgatgact ttaaagcggt
      781 catcatcatt catgaattct ttttctcctg tttcagcttc gattgaacca aaatttagtt
      841 gtccacgaag tttccaacta gctggaacac cataaacagc ttgtactgat gcatcaataa
      901 ctggattgta atgttgtaaa ttaccaccaa gtccaagttc tgcagaaagg gctgtccaag
      961 tatttactga ggcaatacct gttgattgct cagaccaaac tggaaagtta tccgcataaa
     1021 gaacgaattg ttcttgaaga gatttgacaa catcttgatc ttcaaagaag agtactgttc
     1081 cctttgaagc tccaaaacta gctaattttt ccttagttcc agcaatagct gtctctggag
     1141 caccttgtac tttcattgtt tctaccaaat cattagcaac caattcatcc cagaacttag
     1201 taacttcatc attcaataaa atgacaacac gggaagtttg actattaaaa gcagatgggg
     1261 attggcgaac tgcctcttta ataatctcta ctattttctc atcagaaact tctgtattgc
     1321 gaccaagagc gtaaatagag cgacggtttt ttaattcttc caaaaatgac atattttttc
     1381 tccttgttct tcaatctcag atatcttact acatttgttc tagtgacata tgaattagat
     1441 taaagttatt tattactatg tagtaattat attaccacta tatcgtaaag tcaaagaatg
     1501 tgactcacaa ggttttaaat ttttttaaaa ggttaatcaa gtcttcttgc tctgtttctg
     1561 ttaagactga aaaagcatct tctacacgtt taatatgctc tggtagagct ttttttatca
     1621 cttcttctcc ttcagttgtt aaactcacca aaaaggctct cttatcattc ggacaagaat
     1681 ggcgtaatac ccatcccttt ttttccatat ttttaataac aacagtcata ttacctgatg
     1741 tagcaagcat attttcaatc aattctccaa ttttcattgt gcctttagca tagagaacat
     1801 ccaaaactgc aaactgagtt ggtgttaatt caaattcttt aaatgattct gagacttttc
     1861 cgtctattgt tctaaatgct tttcgcatga caaccataga tttaaccgct gaatttttaa
     1921 agtttcccat ttcatctccc aaacgtattt tttcttattt tatcactaat tagttatttc
     1981 aagcaagaat atcatctttg ccctatcaaa agcagctggc aaataataag gttgagtaac
     2041 tactcaacct tattacttag aagaaatcat caaaaagact caactgattg tcttctggta
     2101 aatttcccaa aatacccatc tcatcaaatt tttcaactaa ggtacttgag actcctcctc
     2161 gtttacgaag ctctgttttt gataaaaatt caccatcttc acgtgcccga actatctgtt
     2221 ttgcaacgtt ttctcccaag ccttccatag caacaaatgg agggattagc gtatcctctt
     2281 caataataaa gtcagtcgca tggctacgat aaagatctaa tttcccaaat ttaaagccac
     2341 gctcaagcat ttcattaacc aactctaatg tcgtaaaaag atcattttcg acattcgtcg
     2401 cctcattacg ctgacgcttc tcagtgatat ccttcatgcg cgccttaacg gcatcaagtc
     2461 cagcactcat tgtgcgtaat tcaaaagctt tggcgcgaat agaaaaataa gcacaataat
     2521 aaaatattgg ataatgtact ttaaagtacg ccacacgtaa tgccattaaa acataggctg
     2581 ctgcatgggc tttagggaac atatacttga tttttccaca cgattcaata taccaatccg
     2641 ggacattatt atcacgcatt gcttggatat aaccattacg ctcatcttct gaaattttca
     2701 accataaccc cttacgcacg cgctccatga ttgtaaaggc catctttggt tgtaggccag
     2761 cgtgcattaa ataaaccatg atgtcatcac gacaaccaat aacagttgat aaggtagcaa
     2821 taccttcctt gattaagtct tgagcattac ctaaccaaac atccgtacca tgtgatagcc
     2881 ctgacagttg caacaattct gcaaatgtgg ttggatgggt ttcgttcacc ataccacgaa
     2941 caaagttcgt accaaactct ggaataccaa gcattcctgt aggagttcca atttgctctt
     3001 ctgtcactcc taaaacttcc gtaccagaaa ataacttcat aacgtctgga tcatctggaa
     3061 ggatatttga tggatcaatt cctgataaat cctgaagttt acgaatcata gtaggatcat
     3121 catgtcctag aatatctaac ttcaagacat tctcatcaat atcatggaaa ttgaagtgag
     3181 ttgtctgcca agcagctgtc atatcatctg ctggatattg aaccggagta aaatcataaa
     3241 catccatata gttaggaata acaacaatac caccggggtg ttgacctgta gaacgtttaa
     3301 ctcccgcagc accagtcgct aaccgttcga cctctgcatc gttataaaat ttattgtaat
     3361 ctcgttcgta acctttgaca aaaccaaagg cagttttttc agctacggta cctaccgttc
     3421 ctgcacgaaa agcatattct tctccaaaga tatcacgaac gtctaagtga gcagaaggtt
     3481 gatcatctcc agagaaattc agatcaatat caggaacctt atctccatca aatcctaaga
     3541 aagtttcaaa cggaatatcc tgtccatctt tcttgtacaa agtaccacat ttaggacaat
     3601 ttttatttgg taaatcatag cctgaaccac aagaaccatc tgtgataaat tcagaatgct
     3661 gacaatttgg gcaaacataa tgaggtggca ttggattaac ttcagtaatt cctatcatgg
     3721 tcgctacaaa actcgaccca acagacccac gtgatcctac tagataacca cgttcattag
     3781 aacgttgcac caacatttgc gaagcaagat agataacggc aaatccatta ccaagtatag
     3841 atgctaactc tttttcgata cgtaaatcaa taatatcagg aagaggatta ccatatattt
     3901 cgaatgcttt agcataagtt aattcagcaa ctcgttcttc agcacgatca acaaacggag
     3961 tatagagatc cccttttaca acttcaacgt cttcaaatcg atctgcaaaa gtattcgtat
     4021 tggttacaac gatctcatag gctaaatctt taccaagaaa ggcaaattca tctaacatct
     4081 catttgttgt gcggaaatgt gcttttggca aaggcgctgg ctgagcatcc tccccacgac
     4141 ctatagtcct attaatcatg gcaccttgtc caagactacg aacaataatc tcacggtaaa
     4201 tctcatcttc aggctcaata taatgaacat ttcccgtagc taaaacaggc ttatctaaac
     4261 gccgaccgac ctctattagg tctctaataa tttgttgaat ccccacctca tctttaatta
     4321 aatcacgtac taccaagggg cgataaatag caggtggcat gacttcaata aaatcataat
     4381 atttagctag ggtcactgct gcatcaattc catttgataa caaggcatca aaaacttctc
     4441 catcagaaca ggcagtacct agcaagagac cttctctgtg agcatctaaa actgacctag
     4501 gaattcttgc caccccttca aaatatttaa catttgaaag gctaactaac ttaaatatat
     4561 ttttaagtcc aacctgattt tggacataaa tggttgcatg ctttatgcgt gcctttttgt
     4621 aagagtcttc agctaccaat ttagtattga gctccatcaa attagtcaca tcacgatttt
     4681 cccgcgcctc ttttaagaaa ataaagagta agcgacccgt tgcttcagca tcgtagttgg
     4741 ccatatgatg atgttccaaa gcaacttgga aacgtttggt tagaggcccc aaaccatgac
     4801 gcttatattc aggataaaga tttcttgcaa attctaaggt atcaatgacc ggctgtgtga
     4861 tcaaaggcag attatgacgt tcataattcg cattcataaa accaacgtca aaggttgcat
     4921 tatgcgcaac taaaaccgta ccttgacaaa aattctgaaa ttcctgaagg acttgtaaaa
     4981 taggttttga acctctgaca tgattatccg taatacctgt caattcagtt gtaaaggcag
     5041 aaagaggatg acctgggtca ataaactcat caaattgctc aataatatta cccttgaaca
     5101 tttttgatgc tgcaatctga attaggtcat tattagctgc agaaagacct gtagtttcca
     5161 catcaaatac aacataagtt gcttcgtgca aattcatatc aacttcgttg taggaaatag
     5221 gcactttatc ttcaacaata tttgcctcta atccaaaaat tgcttttata cctgcttttt
     5281 ttgctctgtg gtagccatgc ggaaaacttt gaacattggc atggtcagta attgcaatag
     5341 ctgggtgtcc ccacttagct gcagtatcaa tgaggctctc gacagttggt aaagcatcca
     5401 tcgttgacat attggtatga gcatgaaatt ccacacgttt ttgatctgct ggcattaagt
     5461 ctttacgttc atgatgcaca atctctttga tatcttgaac attcattgtc aaagatttag
     5521 taaagttatt gttctcaata ttccctctga ctcgcaacca cgaaccttta gaaatcatat
     5581 cgtacttctt caattcttca tcatctttag cccacttttg catagcaaaa cttgaggtat
     5641 aatcagtcat cttaaaatta atgatatgac gtccagtgcg ggttgttttt cgttcaacac
     5701 taaaaaccat tccttcaaaa acgatacgat tttcttctgt tgtcacctca atcatgggtg
     5761 taatctctgc tttttcaaag ccagcttgac gttgtttgat acgctctttg aaatcataat
     5821 tttgagttgg ggtcacttct tctgatggag gcgctgaatc ttctagtgat ttttgtgctt
     5881 ccaaggcttg catagcttct tggttagctt tttcaagcaa ttgctcgcga ttagtttcaa
     5941 atgatgattt taagtcttgc gtcatttgtt catcagaaac catatcaata gcaagtttac
     6001 caaatccaaa caatgaaaac tgttgttcca aacgcggcaa atgattctga cgaaaatgat
     6061 tattattgac aaattgaggt gctgaaataa tcatctgact gccattgtaa tgaactttta
     6121 attgtgaaaa agaactctta aaacttgcac tattacacaa gggttcacaa aaagcttgtt
     6181 gataataatc ctgtagcaga tcatcagaaa aatcaatagt ttctgctctt atatcaaaag
     6241 tagcctttat atctgctttt tcaaaactat tcactaaacg agtttgtaat tcacgataca
     6301 cctcaattgg caataattct ggaaatgaaa aatgaaattc ccataagcgt gatagagaat
     6361 gaacctttac ctcgataata tcagcagacg aaaaaacact tgaattctta atttcaagcg
     6421 gcatttcaat ctgatccata agtttcttga acaattctga catagttttc tcctgaatat
     6481 cttacttcca ttttatcaca tatctgtaaa tatttcgaga taacaaaagg aggctgttaa
     6541 aaaaggaatt tattagcctt cttaacaaca ctctcttaac tgaaactatt tatcataagc
     6601 atctagactg taggtttcaa taatttggtt taatttacta gcataagcag tatctgtcgc
     6661 ataaagacct gttagagctg cagtagcatc cttataagaa gaggtatttg atttccaaac
     6721 agatgcatac ttttgactag atactaactc agcataatca tatagtgaag cagaataatt
     6781 tggataggcg cgaaaaggag cttggatttg agtcatattg cctttcccat catcttctaa
     6841 agtaggcatt tggacagatt tacctttata ttctcctttg atgccaaaga ggttataatt
     6901 aggagcctta gacaaatctg attgtccact ggatgattcc aaaatagctt gtgctaataa
     6961 gactgacgca tacaaatcat aggtcttaga aatttcaata gctgttggag aaatcgtttg
     7021 aataaaagtc gtcgtttgat ccgatgcact agtgactgtc ggttcgacat tatcattttt
     7081 ataattataa aaccaaaccc cacccaaacc aaaaaccaat agtgttgttg ttaacctcaa
     7141 taccaattta tctttttttc gacttttcat gatggctcct ttctaaaata tcatttcatt
     7201 ataacaaaag gcagttgctt tttaccaata agaaccatac taaatttcac tccatataaa
     7261 aaagttagca catcagacta acttttttat cggcttattt ttttgttaga atctctaaag
     7321 tttcaattaa gttatcagcg tgaacctcaa tcgtatcacc acttgcttta attttcactt
     7381 caacgatacc ttcagaagct ttcttcccaa cagttactcg aattggaagt ccaattaagt
     7441 cactgtctga gaatttagaa ccaacacgtt cattacgatc gtctgtcagg acttcataac
     7501 ctttaagcat caaatcagct tcaattttct cagtaaggtc ttgtgactct tgatctttga
     7561 cattgacagt aatcaaatgt acatcaaatg gggctaattc ttctgggaaa ttaatccccc
     7621 aagcaaagcg ataagctcct tttggtgtct tattcacaaa aagtcgggca tgttgttcaa
     7681 tgacagctga gagaatacga ctaacaccaa taccataaca ccccataaca ataggatttg
     7741 agcggccatt ttcatcaagg atgttcgctc ccatactatc tgaataacgg gtacctagtt
     7801 taaagatatg tccaatttca ataccacgag caaatttcag agtaccttta ccgtctggag
     7861 aaatctcccc ttcttttact tcacgaatat caacgtattc agctgtgaaa tcacgttctg
     7921 gattaactcc agtaaaatga taaccatctt ggttagcacc agacacagca ttagcaaggt
     7981 cttgaacttt acgatcagca ataattttaa cagagtcagg taggttaact ggcccaagag
     8041 aaccaaaacc tgcaccaaaa atctcttttg cttgctcttc acttgcaggc tctaagaaat
     8101 cagccgctaa ataatttttt aacttcacat cattgacttg atcgttacca actagtaaag
     8161 caaccacggg ctgttcatca gcaataaaca atagtgtctt gatagtttga ttttcatcaa
     8221 ttgataaaaa tccagctacc tcatcaattg atttgcagtt aggagtttct acttttgtta
     8281 cttcttcaaa ggtagcagcc ttagtagatg gtttgtactc attcgttgcc atctcaaggt
     8341 tagcagcata gcttgattca gtagagtatg caatagtgtc ctcaccagaa actagccaag
     8401 ccgagagttc taccttaatt tcttctaaaa catcctctgg aatatcatca atagaaggaa
     8461 tagttttatc aaggactaac caacgattta aatctgtacg attgggagtt actgccataa
     8521 attcttggct atctttacct cccatagcac ctccgtcacc tataatgcct ttaaagtcaa
     8581 gtccagcacg tgtgaagatg gcttcatatg ctttacggta atcttcgtaa gtgacatcaa
     8641 ggtcttcata atctttgtgg aagctgtatc catctttcat gataaattca cgtgttcgaa
     8701 gaagtccatt acgaggacgc ttctcatcac gatatttaga ctgaatttga taaaggttta
     8761 gtggtaattg cttataagat tttactgcat cacgaacaag agttgtgaaa gtctcttcat
     8821 gagttggtcc taagataaaa tccgattgat cacgattctt taatttataa aggtcttcac
     8881 catatgtttc gtagcgccca gattcacgcc ataaatctgc tgtaagaaga gctggagcta
     8941 acatttcaac tgcaccaatc ttctcaaatt cttgacgcat aattgtttta aatttttcaa
     9001 tagtacggtt agctagaggc aaataagcat agattccagc cgaaacttgg cggacatatc
     9061 ctgcacgaac cataagagca tggctgataa cctgagcatc acttggcatt tcgcgaagtg
     9121 ttggaataag cattttactt tgtttcatta caataagata cgactaatca aagttgattc
     9181 tttttcaatt gagaattgca attgaaaaat tcgtatttcc tttcacgttt tattatcttt
     9241 tagaagaaag ctcgcatgat atcgttccag gtcacagcaa tcattaatac taacataact
     9301 gctaccccag cgagcgtaat ataagtttct gtttcttgtt taagtggttt gcgacgaatg
     9361 gcttctaaaa tattcatcac tatcttaccc ccgtcaaggg ctggtatagg gatgagattc
     9421 ataataccta agttgataga gattaatccc attaagttga gaactgtgac gaaaccattt
     9481 ttagcagctt ggctactagc ctgatagaga gctactggac ctcccaattt attaatacta
     9541 aaatgagcaa taagtttctt taattcattt aaaattctaa agaaactttc ccatgctagt
     9601 tttaatccac ctaaaagttt gtctttaaag cttgtcttta aagctggcat aatgccgatg
     9661 atgtaggatt tatcaacttt ctgtggctta acgttgatcg ttttcaccac ctctttgctc
     9721 ttaatcttaa gggcaagttt ttgttttttt tctaaatgcc tagttgactt ttctactgct
     9781 gctgtcaact gttcccagtt agaaactttg tgcgacccta tttgtaaaat acgatcatta
     9841 ttttttaaac ctgcactagc ggcaggacca ttttctgata cacggacctg attggttgat
     9901 aagtcttgaa cacctccttg aataaaagca agtgcaataa agactacaag acctaaaata
     9961 aaattattca tcggacctgc aaaattggta attaagcgcc cccatacaga agcattctga
    10021 tactgcatat ctaagggagc aatacgaatt tcagtaccat cctcctcaat gatagtagca
    10081 tcgtgatcaa cactataggt ttttgtctca cttaagacca atcctgtgat agttagttta
    10141 tcttctaaat cataagctgt tacattgatt ggtaaagaag tattgtctaa ttgttttcct
    10201 gataaattaa tacgagttac aatgccttct ttatttaaag taaggctagc cggcgtccct
    10261 gtcttaattt cagtcttatc atcaccccaa ccagccatac ggacataacc acctagcggt
    10321 aatatacgaa tagtataggt tgttccttct ttatcaatgt gagaaaagat ttttggaccc
    10381 ataccaatag caaattcacg aacaagaatt cctgatttct ttgcaaaata gaaatgccca
    10441 aattcgtgaa caactacaat gacaccaaaa ataataataa aggttaaaat tcctagcatt
    10501 caattcctct ctaaatacaa tatattattt tagaataagc caaagaagtg catgattggg
    10561 aaaacaaaaa tcatactatc aaagcgatcc aaaatccccc catgacccgg aatcaattta
    10621 cccgaatctt taacaccaaa atgacgttta atactacttt caactaaatc tccaaattga
    10681 ccaaaaatgc taaagatagc cactaaaact aacatgacta ggaagctatg aggcgcatag
    10741 acggtcttat caaaaagcat aaaaaagaaa gctaccacaa tggctgaagc aatccctcct
    10801 agactccctt caattgtttt atttggagac actgagggta gtagtttgcg ttgtccaaat
    10861 tgacgaccaa tcatataagc accgatatca gtcgcccaga caataaataa agctaacaac
    10921 accttatcta ttccagccat acgagcactg actagatttt gaaagccaat acccacataa
    10981 aaactagacg caattggaaa ggcagcatct tcaaaactgt aagaattact gttcaaaaca
    11041 gtacctgcca aaatcataaa aataacaata ccgtatgctg ataaactagc gtcaactggt
    11101 aaaaaggaca aataactatc taaaggaact gttaaaacaa aggctcctat catagctaat
    11161 gcaccttcaa aggaaaatat ttctaaacga cgcatacgaa gtaattcaga aacaccaatc
    11221 atagctaaaa gtccaactaa aaattggaaa ggcaaaccac ccattactaa aaaagggata
    11281 aatatggcaa gcgcgacagc tccccaaata acacgttctt tcattttctc tcttctttct
    11341 atacactacc aaatcgacga tggcgttgat tataatccac aatggcctta tgtagttcgt
    11401 cttttttaaa atctggccat aaaacgggag taaagtaaaa ttcactataa gctgattgcc
    11461 aaggtaaaaa gttactcaag cgtaactctc cactagttcg gataatcaaa tcagggtctc
    11521 tataaagata gggtaagcta tttgtcatta aatgatttgc tactaaatcc tctgtaatat
    11581 cgtcaggatt caacttagct tccaaaacat cctgagcaat ttccttaatg gcattagtga
    11641 tttctgaacg tccgccataa tttagcgcaa aattcaagac gagccccgaa ttatgttttg
    11701 tcctcaaaca agcgcgttgc attgcatcgt atgttgcctt aggtaattta tgagtatctc
    11761 cgatgacctg tacacgtaca ttatttttat ctaactctgg tacatattta tcaaaaaatt
    11821 caaccggaag gttcataata aacttaactt catcatctgg acgtgaccaa ttttctgtag
    11881 aaaaggcata gacagttagt actttaacac ctaatcctga agctgctatt gtaacttctt
    11941 gaagagcatc catccccgcc ttatgtccca taacgcgtgg ttttaaacgt ttttttgccc
    12001 aacgtccgtt accatccatg ataataccaa tatgtgctgg tattttctcc aaaggcattt
    12061 ctacttgttt ttttctttta aatataaaca taatgttcct taatttagca atcgtttttc
    12121 ttatcagccc ttttaaaaga gctggaaata tcccatacta taacatttta ccacatttca
    12181 acttattttt gagtattaaa atgccatttg tgacagcaaa ctaaaaagac cagaacaatc
    12241 atctggtcct acatatctta ttcagtttcg atggctgttt ctttattttc gtcttcattg
    12301 acttcaataa tcttaacatc ttcaactggt tctgttggtg ttgctgcctt agagacaaca
    12361 gatttgatag ctgccaactc aaacgtcaaa tagacaccat caacatctaa aaccattcgt
    12421 tgtgcttcag tattaacttc atcaactact ccaaaaagac caccaattgt gacaatttca
    12481 tcaccttttt gaacagcatt caattgtttt tgacgttctt gtgcttgttt cttttgctga
    12541 cgttgcatga agaacatcat accaaccata acaacaagca taataatagg taagctcatt
    12601 tgattctcct ttccgtgttc tacgtaatca ttactatagc agatttatgc tatatctgca
    12661 aggagatact gtctcttagc caataaattc acggatttct tcttttgtca tttttgaatc
    12721 gcacttcacc ttgatagttc cgtttttaac aaccatcaaa cctggcactg taggaatgtc
    12781 atatttattt cgaaattctt tatttgcagt ccaatgatca aaatcttcac tatctaaatg
    12841 gaataattca aattcatctt catcagctac ttctacaatt ttaggaacaa agaggtgaca
    12901 aaatggacaa gaaatacgac caatgaacag tacaaaatct tgatttgaat caattttatc
    12961 ttcaatttcg gcagccgtta tcaaagtcat ggttgccaaa agttcttcaa aagaatacat
    13021 agttaaccaa ctttctaaat cttatattta aagaataacc tatgatggca aaacttgcaa
    13081 ggcaagagta acaacgatcc tatcaaaatc attaactcac ttctcgcttc aaactatcct
    13141 caaaaatgct cactcatatt taaatgactc atcaaaaaaa ctgagcatag ctcagttttt
    13201 attttacacc ttatttttgt acagcattac tgacgctttt agcaactaca tcggccacat
    13261 cattttgaaa agcatttgga atgatgtgag ttgtgcttaa ttcttcctcc ggtattaaac
    13321 tagctatgcc tctcgctgcc gcaatttgca tttcaacagt tatcgttttt gctctagcat
    13381 ctaatgctcc tctgaaaata cccggaaatg ctaaaacatt attaatttga tttggaaaat
    13441 cactacgacc agttccaact atgtaagctc cggcttttaa ggcttgatct ggataaattt
    13501 ctggaatagg attcgccatt gcaaatacaa taggtttatc agccatcttg gaaatccatt
    13561 ctgcatgtaa tgcttccgga gcagaaacac ctataaaaac atcggcattc tccaaagcat
    13621 cttctaagga tccagattgg aattctctat tagtcaattt tgcaatcgct ttgtgatgag
    13681 gggctaatga ctcccgatct ttatcgttaa taatgccaaa tcgatcaact accgttacat
    13741 gttttgctcc tgctgataga agcttccttg ttatagataa gcccgctgaa cctccaccat
    13801 taacaactac tctgatgtct tcaatgtctt ttttaactag cttcaaactg ttaaacaaag
    13861 ccgctaatac tacgattgct gtaccatgtt ggtcatcatg gaaaactggt atatcacatt
    13921 cttcaattag gcgttgttca atttcgaaac aacgtggtgc tgaaatatcc tctaaattaa
    13981 tccctccaaa agtcggcgaa atagctttta ctatagaaat aatttcctct gtatcatttg
    14041 ttttcaacac aattggcaca gcatctacat ttgcaaaacg tttaaagagt gctgctttac
    14101 cttccatgac tggcattgct gcctcaggac caatatctcc taatccaaga acagctgaac
    14161 catcactcac tacggctact gtattttttt tagtcgtcaa ctcatatgca agctccacat
    14221 cctctgcaat agctgatgat actgacgcca cccctggggt ataggcaata ctaagatccc
    14281 tcttatcttc caccttaact tttgaaatta cttctaattt tccaccattt tctctagctt
    14341 ggttgatagc taattgacct aaattttcac tcattttgat aactcctttt gataaatatt
    14401 agaataacat gcgtaaaatg gctgtcattg tgataacagt aatagctcct cctaaacgcg
    14461 tagcaacttg agcaaatggc atcaagttca ttcggtctgc tgtacttaaa atggcaacat
    14521 ctcctgttcc tcccatacca ctttgacaag ctgaaataat acctgcttca acaggattca
    14581 tattaaggaa tcgcgataca aagaatccaa cagaaataac tgtaaataca acactaataa
    14641 caactatgaa atattgtatg ctaagcgttg ccacaacatc ttttaacggg atataaagca
    14701 atcctagacc tgccattaga ggaaatgtaa aattaccaga aataaattta taaagttgct
    14761 tagctccatt ttgtgtttct ctaggaataa cattaatata tttcaaaata gctgctgcaa
    14821 caatcattaa tacgggacca ggaaatccag ttaattgctg caacaaatgt ccaaaaataa
    14881 agagactaca tgctgttaaa acacctccac ccattttctt aacgtcaatt gggccagaat
    14941 gatcagcaat aatatctgac atgtcctctc ctttatttaa ccttactaat tgcccttgac
    15001 cagacaagtg tggtttcttt tctcccaaac gattcaatag tgcagtacat aaaatggcaa
    15061 agaaattacc aataatagtg gctgggatga gttgagcaac tagttgttca ctagctacac
    15121 cggtaattga actatagcct aacgataatg gtaaaatacc ttcaccaata ccaccagcta
    15181 aaacgggtgt tacgatataa aacaaagtgt gtcgccactc taagcctaaa ataacaccga
    15241 caaatgtccc taccatcatc gcacaaacca ttcctaataa catggggaaa atcattctta
    15301 gcaatccctg aatcaacatt ttccgattca tccctaaaat actaccggac actaaacaag
    15361 caatataaaa ataaagaaag ttagcttgct tcatcaacat atttgttgat tctaaaacat
    15421 ttttattaat gaggttgaaa aacaccaaaa tagatggtac taataaagat aaaatagctg
    15481 ggccaccgaa gtttttaaaa ccaggaatgc tagctccgat agtccctaag aaccacccca
    15541 ttgttaagat aactgcaaat cctcctagca tattgactgg caatttttgt aaaaagcctg
    15601 ctagaagaat caataatgct aaacaaacat atactggtag gggaactgac cctatcttaa
    15661 tatgaaatag tttagccttt aagtcttgct tttgacctct agaatcctca ttattcacta
    15721 ccttcacgtc tgccatgaaa gcacctcctt ttacttctta tgtacagttt actttgggac
    15781 ttagtgataa ggaagtttat ttaagtaatt gaattatttc aacttattta attaatttaa
    15841 gctggattct agtttttagg ctatgttata ctaaactcgg agggtgctga tgaaaaaaaa
    15901 actatctctt tgggcatttc tatcattgat tttagtcact atgaccattt gtatattttc
    15961 tattttttat tatgtcacta tacatcagtc ctatcgtatg gtaagggtac aggaagaaaa
    16021 aatcttaaaa aatactggtt atgctttatc gagaaatcca caagttatac aaacactcaa
    16081 agataatcac tatgatcaaa gtctccaaaa acaaatgtta ttccttagca aaaaatcaaa
    16141 tttagactat attgtcctta taaatttaaa agggatccgt tttacccacc ctgactccac
    16201 taagattggt aaaccatttc aagggggtga tgaacaagct gtttttaagg gaaaagctat
    16261 tatgtctact gcagaaggtt ctttaggaaa atctttacga tacttaattc cagtatatga
    16321 tcaccaaaaa caggttgggg caattgcagt tggcttaaaa ttaacgacac ttggagactt
    16381 atctcaaagt agcataaaag agtttagtaa gcctctcttg attagtattt tgattagctt
    16441 agtcgtcaca agtataattt cttatggctt aaaaaaacaa cttcacaacc tccatccaag
    16501 tgatattttt caacatttag aagagcgcaa tgcaacactg gaccaaattc aagccgctgt
    16561 atttgttatt gaccagaggc atatcatcaa aagaaataat cctgcagcta gtttattatt
    16621 caaaaaagag gggcaacgag acctattttc aggtaaatta ttagaatctt taattcctca
    16681 actcaagcag gatcattttt caaaaaaaac tgaacaagta ttacattttc aaggacaaga
    16741 ctatttacta tctatctcgc caattacggt taaaacacaa aatagaggct atgttgtctt
    16801 cttaaggaat gttactgaaa cacttttcac tttggatcaa ttagcacaca ctacagctta
    16861 tgcttctgcg ctacaggccc aaacacatca atttatgaat caacttcatg ttatttatgg
    16921 acttgcagac attgaatact acgacgagtt gaaaatttat ttaaaagaat tattggagcc
    16981 acaaaatgaa tttttagcac gcttatcaat gttagttaga gagcctcgac tggctagttt
    17041 cattattggc gaaagagaaa aattcgcaga aaaacacatc aatctatcca cagaaatttt
    17101 agtagaaatc cctaccaagt caactgttga ggatgttaat aactatctct tgctccacag
    17161 atatattaat acgaagatac tcacgctatt aaattcaacg actttagttt ctttacgttt
    17221 aaattatcaa aataacctca ttgaaacaga ttatcaatgg gagaatgaga agtggttact
    17281 caatgactac catcagtatt tcaacgacgc ttattttcaa cagttacttg tagattcaag
    17341 agctacttat aagatctctt taaaacaaaa taaggttatt ataagaacaa atattatcta
    17401 ctcagaaggg ccttagtatg gacgtattaa ttatcgaaga tgatcctatg gtggaattta
    17461 tccaccgcaa ttaccttgaa aaactcaatt attttcaaaa catctattca actgctagtc
    17521 aaacacaagc tattgcttat cttaatgaca ttaaaatcca acttgttctt ttagatattc
    17581 atattaaaga aggcaatggt ctagaattat taaagttatt acgcaatcaa caccaaaata
    17641 ccgaagtcat tgttatttca gcagccaacg aggcgcacac tgtcaaagaa gcatttcatt
    17701 taggtattgt agattacctg ataaaaccat ttacatttga acgctttgag tctagtattg
    17761 aaaaattcct aaatcattat catacatttg aagctgataa aatctatcaa gacaacatcg
    17821 atcactttca aaaaatagat tctggatggc tagagggaga ggttaaactt gatgagaaag
    17881 ggctctctga aattacctat cagcatattc ttgatgccat tcaagagtta gagcagcctt
    17941 tcactatcca agagttagca aaatgcagtc aattttctca tgtctctgtt aggaaatata
    18001 ttgcctatat ggaagaaaaa ggccttctga caagtcagca aatttatact aaggtcggtc
    18061 gtccttacaa agtttataag cttataaaaa aaaacttagg cctgtagacc taagtttttt
    18121 taatcttcgt agccatttgg atgactagaa tgccatgccc atgccgatga aataatttta
    18181 tcaatattat caaatttagg ctcccaccca aggatttgac gagctttctc tgaggaagca
    18241 ataagcgtat ctggatctcc tggacgacga gctgcctttt gagcaggaat ttctttccca
    18301 gtaatacgac gagctgcctc taacatctga aggttagaaa aacctgtcga agatccgaga
    18361 ttaaacacgt ttgattcatt cccttggcga aggtaatcaa ctgctaatat atgagcatct
    18421 gccaaatcaa atggatgcac ataatctcga acattagttc catctggagt attgtaatca
    18481 tctccaaaaa tcattatttt gtcacgtact ccttgagcaa cttgaagaat gattggtaac
    18541 aaatgtgttt ctggtttatg atcttcccca attgagccat ccggtttatc tccagcaacg
    18601 ttgaagtaac gtagagcgac aaacttaata ccgtaggctt gatcagccca tttcataatt
    18661 gtttccatca taagcttact ttcgccataa ggattaatag gattttgagg agctgtttct
    18721 aaaataggaa cttgttctgg aataccatat gttgcagctg ttgatgaaaa gacaatattt
    18781 ttgatatcac attcattcat tacttctaaa agttttatca ttccagcagt gttattatca
    18841 aaatatttta aaggattttc cattgactca gcaactagtg aaaatgccgc aaaatggata
    18901 acagcatcta catcaggatt ttccctaaaa acttgacgca taaacgtttt atcggataaa
    18961 tctccttcat aaaaaatcgc atctgaatgc accgcttgac gatgacctgt taccaagtta
    19021 tcaacaacaa taactttctc ttttccttgt gtgattaact gatcaaccat atgcgagcca
    19081 atgtagccgg cgccacctaa aatcagaact gccatatttt tctcctattt caatactctt
    19141 ttaacatcat ttgttacctc attatactga gaataagctt tttttgcaaa tatctaccga
    19201 aattagaata gctctatgca gaaggcatcc cacggcttga gagtgttact tgatagtact
    19261 tcttgaacct ctgtatttga aataatagtt tctttaacag cttctggaaa ttcaaaagat
    19321 tggttttgat cggataagtt cgcaactatt aaataacgtt ctttatctgt ttgacgaaca
    19381 taagcaaata ccttatctgc tgtttccaaa agtttaaaat cagcatcaac taaccagtcg
    19441 tgttctttac gtaaagcaac taatttttga taagtataaa aaatactttc cgtatcattt
    19501 aacgctgcct ccacattaat ttcctgataa ttgggattga ctgctagcca tggggtacct
    19561 ttagtaaaac cagcttgatg ttcttgactc cactgcattg gagtacgagc attatcacga
    19621 ccgactttac gaatactatc taaaattgtt gcttcagata ccccattatc catggcttct
    19681 ttagcatagt taagggattc aatatcatct acatctgcta aacactcaaa tggataatta
    19741 gtcataccaa tttcttcacc ttgatagata taaggcgttc cacgcatcaa atgtagtagg
    19801 attgctagag ccttggcaga ttgttttctg ttatcactat catttcccca gatcgagagc
    19861 actcgaggaa ggtcatgatt attccagaat aaagaattcc aaccttggcc aagctccaat
    19921 tgtgtttgcc attttgtaaa aatatctttc aatgctggaa catctaatcc gtcactgtaa
    19981 tcccacttag gagcgtcagg tttatgttga agacctacat gttcaaattg aaagaccata
    20041 gataattctt cattatcggg atttgagtat tgttttgcaa tgtctggcgt tgctccccaa
    20101 gtttcaccca ccgtcaataa gtcatgcttt ccaaaactag ctctgttcat ttctttaaga
    20161 taatcatgaa gttttggtcc attaccagta atgcccttat ctggaatttt cccaattaaa
    20221 tcaatgacat ccatacggaa accaccaata cccttatcaa tccagaaatt catcatatca
    20281 taaattttat gacgtaaagc ttcattctcc cagttgagat ctggctgacg tttgctaaaa
    20341 aggtgcaaat aatattgacc tgatacttta tcatattccc aagctgagcc actaaaaata
    20401 gaagtcaaat cgttaggttc atctcgccaa atataaaaat cacgttcagg actattaggg
    20461 ttttctcgcg cttctacgaa ccaagcatgt tcatcagagg tatgattcac taccaaatcc
    20521 atgatgattt taatcccgcg ttgatttgcc tcttgtaaca gttggtccat atcattcata
    20581 tcaccaaaaa tatctgcaat ggcttgataa tctgaaatat cataaccatt gtcatccatt
    20641 ggactttgat aaacaggtga taaccagata gcagtaatcc ctaatttttc aaggtagtct
    20701 agtttgctag taatcccttt aatgtccccc acaccatcac catctgaatc cataaatgaa
    20761 cgtgggtaaa tttgataaat cgtagcttta tgccaccagt gtttcttcat tattcttctc
    20821 ctatcaataa ttcattcatc ttttatttta acgtttttcc tcttttagtg caagcgtttg
    20881 cgtaaaaaca ttataaaaag gctggaaccc aaccttttta tgaatatcgc ttatacctta
    20941 acgaatagct tgttcagtat ctgcatcaaa gaaatgcccc ttagcaacat taaacgttag
    21001 gttaactttt tctcctggat tatggaagtc acgcgcttca acacgagaag caaattctgt
    21061 ttgacctaac ttaacataaa gcatagtttc gctaccgagt aattcagaaa ctaaaacttc
    21121 tgcttcaaca ttggcatttg gataagtatc ttggacaaga agattgcttg aaatgtcttc
    21181 tggacgaata ccaaaaatca attcttttcc tttatagcct cgtgattcta aaagtttttc
    21241 ttgaccttca gggatagcga ttcgaagacc atcttcacta ataatcatac cattttcaac
    21301 tttaactttg aagaagttca tagatgggct accaatgaat cccgcaacaa atttattggc
    21361 aggaagatta tagagttctt gtggtgaacc aacttgctca attttaccaa ttgtaccatc
    21421 accatcaggg ttcttagtag ctgacataat gacaatacgg tctgccaaag tcatcgcttc
    21481 tgtttggtcg tgggtaacat aaatagttgt tgaaccaata cgttgatgaa tttttgcaat
    21541 ttcagcacgc attgaaacac gtaatttagc atctaagtta gataaaggct catccattaa
    21601 gaacactttg gcatcacgca caatagcacg tcccatagca acacgttggc gttgtccacc
    21661 tgacaaatcc gcaggtttgc gctctaagaa ttcagtaagg ccaatattag ctgctgcttc
    21721 acgaacgcgc ttatcaattt cttgttttga gaatttacgc aatttgagcc caaaagccat
    21781 attatcgtaa acagtcatat gtggataaag agcatagttt tggaaaacca tagcaatatc
    21841 acgatcttta ggtgatttgt cattgacaac ttcgccatca atcttgagct caccttcact
    21901 aatatcttca agacctgcga tcatacgaag tgttgttgat ttcccacaac ctgatggtcc
    21961 aacaaaaaca ataaattctt tgtctttaat atccaagtca aagtcttcaa cagaataatg
    22021 cgatgcactt ggatattttt tgtaaatatg atttaaattt aattctacca ttagattgac
    22081 ctcttttatt attgtgattt tattatag
//
LOCUS       DATRTN010000021        21510 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.021, whole genome shotgun sequence.
ACCESSION   DATRTN010000021 DATRTN010000000
VERSION     DATRTN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21510
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.021"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            65..943
                     /locus_tag="VBE22_000870"
     CDS             65..943
                     /locus_tag="VBE22_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7202341.1"
                     /translation="MRESIFEETAKGKVSRHVLLTILLALILLFTADFLTYIIIELII
                     SLDFWKLEWDSDIVQLIILLATSISTVMLFMWVKFKEKRPISSLGLFKEGALTELSRG
                     WLVGSVLFGACLILTIISGSGVIETVRFTADKLIWFLIFAVGWQVQSASVEILCRGWL
                     LPVSATKYSKIVSVSISSIFFGLLHSANNHVSLISIFNLCLFGLFLSLYVILKGNIWG
                     ACGIHGAWNCVQGSVFGIEVSGEPMLSNSLVHVKTYGADWISGGKFGVEGSMITSIVL
                     IVACYWLYQKSNFPYD"
     gene            1050..1640
                     /locus_tag="VBE22_000871"
     CDS             1050..1640
                     /locus_tag="VBE22_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO7202342.1"
                     /translation="MPQNPTSNPEKKTYLLSELVDCFKGKAVPSKAEAGDIRIINLSD
                     MSPLGIDYHNLRTFQDEQRSLLKYLLQEGDVLIASKGTVKKVAIFEEQDYPVVASANI
                     TILRPTQHIRGYYLKLFFDSEEGQQALENANKGKAVMNISTKELLNIAIPSIPLFRQD
                     YLIQRYKQGLNDYKRKIARAEQEWERIQNDIRQQLS"
     gene            1674..2945
                     /locus_tag="VBE22_000872"
     CDS             1674..2945
                     /locus_tag="VBE22_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEO7202343.1"
                     /translation="MNEIKCPHCGTAFAINESEYHQLLEQIRGDAFDKEVSERLEKER
                     LILGEQAKNQLQEVVVEKDKEIAKLQYKVKQFLIEKDNLLKDNEYQLAEQLNQKDMML
                     RDLENQIDRLRLEHENSLQEALTKVERERDAIQNQLHIQEKEKDLALASVKSDYEVQL
                     KAANEQVEFYKNFKAQQSTKAVGESLEHYAETEFNKVRHLAFPNAYFEKDNTLSSRGS
                     KGDFIYREKDENDLEFLSIMFEMKNESDDTIKKHKNEDFFKELDKDRREKSCEYAVLV
                     TMLEADNDYYNTGIVDVSHKYPKMYVIRPQFFIQLIGILRNAALNTLKYKQELALMKE
                     QNIDITHFEEDLDIFKNAFAKNYNSASKNFQKAIDEIDKSIKRMGAVKAALTTSENQL
                     RLANNKLDDVSVKKLTRKNPTMKAKFDALKD"
     gene            2958..3389
                     /locus_tag="VBE22_000873"
     CDS             2958..3389
                     /locus_tag="VBE22_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7202344.1"
                     /translation="MKIRTATLDDSEKLVPLYQELGYAISLSEIQSILKVILTHSDYG
                     FLIAEDNGKLLAFVGYHKLYFFEKSGTYYRILALVVNEKHRRKGIASQLINHVKQLAK
                     TDGSEVLALNSSLKEYRQEAYHFYENLGFKKVSTGFSYYLD"
     gene            3481..4365
                     /gene="truB"
                     /locus_tag="VBE22_000874"
     CDS             3481..4365
                     /gene="truB"
                     /locus_tag="VBE22_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEO7202345.1"
                     /translation="MITGIINLKKEAGMTSHDAVFKLRKILHTKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTESGKIYEGEITLGYATSTEDSSGEVISRTPLTQSDLSEDVV
                     DHAMKSFTGPITQVPPMYSAVKVNGKKFYEYARSGEEVERPKRQITISEFRRTSPLYF
                     EKGICRFSFYVSCSKGTYVRTLAVDLGIKLGYASHMSFLKRTSSAGLSITQSLTLEEI
                     NEKYKQEDFSFLLPIEYGVLDLPKVNLTEEDKVEISYGRRILLENEADTLAAFYENRV
                     IAILEKRGNEFKPHKVLL"
     gene            4378..5310
                     /locus_tag="VBE22_000875"
     CDS             4378..5310
                     /locus_tag="VBE22_000875"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEO7202346.1"
                     /translation="MKIHYINDYKDIQAKEDCVLVLGYFDGLHLGHKALFDKAKKIAT
                     EKNLKIVVLTFNETPRLTFARFQPELLLHLTSPEKRSEKFQEYGVDELYLMNFTSHFS
                     KVSSDLFIKKYIYGLRAKAAVVGFDYKFGHNRTSGDYLARNFKGPVYIIDEISEGGEK
                     ISSTRIRQLITEGNVEKANQLLGYEFSTCGMVVHGDARGRTIGFPTANLAPINRTYLP
                     ADGVYISNVLINGKYYRAMTSIGKNITFGGTELRLEANIFDFDGDIYGETIEIFWLKR
                     IREMVKFNGIDDLVKQLKKDKEIALNWKKDSQTL"
     gene            5353..5766
                     /locus_tag="VBE22_000876"
     CDS             5353..5766
                     /locus_tag="VBE22_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEO7202347.1"
                     /translation="MITLFLSPSCTSCRKARAWLSKHEVAFEEHNIITSPLNKEELLQ
                     ILSFTENGTEDIISTRSKVFQKLAIDVDELSTSSLMELISENPSLLRRPIILDKKRMQ
                     IGFNEDEIRAFLPRDYRKQELKQATIRAEIEGKHD"
     gene            5759..6040
                     /locus_tag="VBE22_000877"
     CDS             5759..6040
                     /locus_tag="VBE22_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEO7202348.1"
                     /translation="MISSNYSYPLDPSWNTEDITKVLRFLNQVEHAYENSVKVDDLLD
                     SYKEFKKVVKSKAQEKQIDREFQRTSGYSTYQAVKAAQQKAKGFISLGR"
     gene            6030..6794
                     /locus_tag="VBE22_000878"
     CDS             6030..6794
                     /locus_tag="VBE22_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEO7202349.1"
                     /translation="MDAKFDFAKLLVYKAGQFIKSEMQNTFDIEEKSRFDDLVTSLDK
                     KTQKLLIQEIIQHYPDDNILAEEDDVRSPIAQGNVWVLDPIDGTVNFIVQKDNFAVML
                     AYYEEGVGQFGIIYDVMADILYSGGGHFDVYANDKKIVPFQECPLERCLLGVNSAMYA
                     ENDCGIAHLASETLGVRIYGGAGISMAKVMQGKLLAYFSYIQPWDYAAAKIMGETLGF
                     TLLTLDGEEPNYSTRQKVMFLPKSKLNLIQSYLTKK"
     gene            6862..8172
                     /locus_tag="VBE22_000879"
     CDS             6862..8172
                     /locus_tag="VBE22_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEO7202350.1"
                     /translation="MKLPNEFIEKYQTILKDEAEAFFDSFEQKPISAYRTNPLKEKQL
                     DFPNAIPSTPWGHYGKISGKSIEHTTGLVYSQEPAAQIVAQIAEPQEGMKVLDLAAAP
                     GGKTTHLLSYLNNTGLLVSNEISNKRSKILVENVERFGARNVIVTNESSQRLAKCFNS
                     FFDLIVFDGPCSGEGMFRKDPQAIQYWHKDYPTECAQLQRDILKEAIKMLAHGGILVY
                     STCTWSPEENEEVVNWLLQEYDYLELVDIPKLNGMVEGINVPQVARMYPHHFQGEGQF
                     VAKLRDTRSKEAQKIKPKAQKINKMQLQLWQQFAQDHLKIDLNGVLDVFGDQLYLLPN
                     GLPDLSKLKIARNGLHLGTFKKNRFEPSFALGMALSEHDLVQSIEIDIEQFEVYVSGN
                     VVKLDKTVPNGWYQILVKGNGLGFAKVTNNTLKNYYPKGLRFQT"
     gene            8351..9217
                     /locus_tag="VBE22_000880"
     CDS             8351..9217
                     /locus_tag="VBE22_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEO7202351.1"
                     /translation="MKMKKNIFLLTLLATVLLTLSGCANWIDKGQSITSVGSTALQPL
                     VEAAADEFGKTNLGKTINVQGGGSGTGLSQVQSGAVQIGNSDLFAEEKDGIDAKKLKD
                     HQVAVAGLAVIVNKKVNVKNLTTHQLRDIFAGKIKNWKEVGGQDLDISIINRAASSGS
                     RATFDNTIMGNVAPIQSQEQDSNGMVKSIVSQTPGAISYLAFAYVDKSVGTLKLNGFA
                     PTAKNVTTDNWKLWSYEHMYTKGNETGLTKEFLDYMKSDKVQSSIVQHMGYISINDMK
                     VVKDAEGKVTPK"
     gene            9263..10180
                     /gene="pstC"
                     /locus_tag="VBE22_000881"
     CDS             9263..10180
                     /gene="pstC"
                     /locus_tag="VBE22_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO7202352.1"
                     /translation="MKNQELAKKLTSPSKNSRLEKFGKGITFLSLALIVFIVAMILIF
                     VAQKGLSTFFVDGVKLTDFLFNTKWEPSAKSFGAFPMIAGSFIVTILSAIIATPFAIG
                     AAVFMTEISPKYGSKILQPAVELLVGIPSVVYGFIGLQIIVPFVRSIFGGTGFGILSG
                     VCVLFVMILPTVTFMTVDSLRAVPRHYKEASLAMGATRWQTIWRVILNAARPGIFTAI
                     VFGMARAFGEALAIQMVVGNSAILPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLIMSLAFNTVIKLITREGKKNYER"
     gene            10170..11057
                     /gene="pstA"
                     /locus_tag="VBE22_000882"
     CDS             10170..11057
                     /gene="pstA"
                     /locus_tag="VBE22_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO7202353.1"
                     /translation="MNAKKADKLATTILYSIAAIIVTILASLLIFILVRGLPHVSWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSLGAGIYLSEYAKKGRLTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFQYGFSIISGALALTVFNLPQMTRSVEDSLQNVH
                     HTQREAGLALGISRWETVIYVVVPEALPSIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNVLSVTSPISIFRQAETLAVHIWKVNSEGTIPDATQVSAGSAAVLLVVILIF
                     NLSARSIGKKLHSKLTSSN"
     gene            11069..11872
                     /gene="pstB"
                     /locus_tag="VBE22_000883"
     CDS             11069..11872
                     /gene="pstB"
                     /locus_tag="VBE22_000883"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO7202354.1"
                     /translation="MAEYNWDERHIITFPEENSALTTKDLHVYYGEKEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGQIMYEGIDVNAQDINVYEMRKH
                     IGMVFQRPNPFAKSIYKNITFAYERAGVKDKKFLDEVVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAIAVKPEILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTNNIFQNAKCQSTSDYVSGRFG"
     gene            11884..12642
                     /gene="pstB"
                     /locus_tag="VBE22_000884"
     CDS             11884..12642
                     /gene="pstB"
                     /locus_tag="VBE22_000884"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO7202355.1"
                     /translation="MTQPILQVSDLSVYYNKKKALKEVSMDFYPNEITALIGPSGSGK
                     STLLRAINRMGDLNPEVTLTGAVMYNGHNVYSPRTDTVELRKEIGMVFQQPNPFPMSV
                     FENVVYGLRLKGIKDKATLDEAVETSLKGASIWDEVKDRLHDSALGLSGGQQQRVCIA
                     RTLATKPKIILLDEPTSALDPISAGKIEETLHGLKDQYTMLLVTRSMQQASRISDRTG
                     FFLDGNLIEYGNTKEMFMNPKHKETEDYITGKFG"
     gene            12676..13329
                     /gene="phoU"
                     /locus_tag="VBE22_000885"
     CDS             12676..13329
                     /gene="phoU"
                     /locus_tag="VBE22_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO7202356.1"
                     /translation="MLRSKFDEELDKLHNQFYAMGIEAIGQIKKTVRAFVSHDRELAK
                     EVIEDDVTLNNFETKLEKKSLEIIALQQPVSQDLRTVITVLKATSDVERMGDHAAAVA
                     KATIRMKGEERIPAVELEINNMGKAVKNMLEEALTAYINGDDEKAYEVAAMDEIVDDY
                     FRDIQKMVVETIQKHPDVAFAAKEYFQVLMHLERIGDYGKNICEWIVYLKTGKIIEL"
     gene            13475..16024
                     /locus_tag="VBE22_000886"
     CDS             13475..16024
                     /locus_tag="VBE22_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEO7202357.1"
                     /translation="MKTVEHFVTKFVPENYNLFLDINRQTKTFSGNVAVSGEALDNNI
                     SFHQKGLTIKSVLLDNQPLDFQLDEDNEAMHIQLHETGSMVLVFEFSGHITDNMTGMY
                     PSYYTVNGIKKEVISTQFESHFAREVFPSIDEPEAKATFDLSLKFDQKEGEIALSNMP
                     EINAEQRQETGLWTFDTTPKMSSYLLAFALGELHGKTTHTKNGTLVGSYATKAHQLNE
                     LDFSLDIVVRVIEFYEDYFGVRYPIPQSLHVALPDFSAGAMENWGLVTYREVYLLVDE
                     NSSVSSRQQVALVVAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIDYIEPKLN
                     IFEDFQTGGLPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRW
                     LGDTDFAAGLKIYFEKHQYQNTIGRDLWNALSQTSGKDVAAFMDSWLEQPGYPVMAAK
                     IEEDELILTQKQFFIGEHEDKSRLWQIPLNSNWEGIPEILTEETVVIPNFSQLAEKNK
                     ENGALRFNTENTAHYITNYQGQLLEHIISDLPLMDNISKLQIVQERHLLAESGMISYS
                     SLIPLVSLLSQETSYLVNSAIKSVIDGLSLFVQEDSQDEFDFKEFVNKLSAFNFNRLG
                     FEKREGEGDDSEMVRHLSLSLALYSDNEHAIEEAHHIFKAHENNIAAIPAAIRLLVLT
                     NEMKHFESKELSHLLLETYSTTTDGNFKRQLASALSHTTDSKTLKKLLSDWKNKDIVK
                     PQDLAMSWYATFLKNSFTQQSVWEWAQENWEWIKATLGGDMSFDKFVIYPSSSFKTEE
                     RLEQYKNFFEPQLSDMAISRNISMGIKEISARVLLITKQKEEVINTIKKYI"
     gene            16186..16866
                     /locus_tag="VBE22_000887"
     CDS             16186..16866
                     /locus_tag="VBE22_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7202358.1"
                     /translation="MIKILLIEDDLSLSNSVFDFLDDFADVMQIFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESIDDKGQGFDLGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNDNSLIYGDIRVDMSTNSTFVNQTEVELLGKEFDLLVY
                     FLQNQNVILPKSQIFDRIWGFDSDTTISVVEVYVSKVRKKLKGTLFSENLQTLRSVGY
                     ILKHVETD"
     gene            16850..18163
                     /locus_tag="VBE22_000888"
     CDS             16850..18163
                     /locus_tag="VBE22_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7202359.1"
                     /translation="MSKPIKISKFKKNVSDSHFIHFFTVFSGIFLVMTVIILQVMRYG
                     VYSSVDSSLKYISTHPKNYINMVMSRTAAYLDNSNIASVKLKPGGQTVANTDIILFTS
                     EEEVINYFDAFSNYQFLKPNKKNLGGISELTLTNIFGQDETYHAVTVKVNNPAYPNVT
                     YMTAIVNIDQLVNAKERYEKIIIFVMTTFWIISIGASIYLAKWAQKPIIENYERQKAF
                     VENASHELRTPLAVLQNRLETLFRKPNATILENSENIASSLDEVRNMRILTTNLLNLA
                     RRDDGIKPELAVIKPTLFDSIFENYDLIAQENGKNFTGHNMIQDSFKTDKTLLKQLMT
                     ILFDNAIKYTDNDGSIDFTISETDKYLFLEIADNGPGISEEDKVRIFDRFYRVDKART
                     RQQGGFGLGLSLAQQIVNSLRGNITVIDNKPRGSIFKIKLPKVKG"
     gene            18252..18584
                     /locus_tag="VBE22_000889"
     CDS             18252..18584
                     /locus_tag="VBE22_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEO7202360.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     SGVSRQAVYDNIKRTEKILEAYEMKLHMYSDYIVRSQIFDDILEKYTDDAFLQEKISI
                     LSSIDNRD"
     gene            18602..20167
                     /gene="ffh"
                     /locus_tag="VBE22_000890"
     CDS             18602..20167
                     /gene="ffh"
                     /locus_tag="VBE22_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEO7202361.1"
                     /translation="MAFESLTERLQGVFKNIRGKKKLSEKDVQEVTKEIRLALLEADV
                     ALPVVKTFIKHVRERAVGHEIIDTLDPTQQIVKIVNEELTDLLGAETSEIEKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEDNARPMMIAADIYRPAAIDQLKTLGSQINVPV
                     FDMGTNHSAVEIVTKGLEQARENRNDYVLIDTAGRLQIDATLMQELHDVKAIAQPNEI
                     LLVVDSMIGQEAANVAEEFNRQLSISGVVLTKIDGDTRGGAALSVREITGKPIKFTGT
                     GEKITDIETFHPDRMASRILGMGDLLTLIERASQEYDEKRSMELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMLPGMANNPAMKNFKVDENEIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVDVNKFIKDFNQAKQMMQGVMSGDMNKMMKKMGIDPNNLP
                     KDMPGMDGMDMSNLEGMMGQNGMPDLSSLGGDMDFSQMFGGGLKGKVGAFAAKQSMKR
                     MANKMKKAKKKRK"
     gene            20234..20920
                     /locus_tag="VBE22_000891"
     CDS             20234..20920
                     /locus_tag="VBE22_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEO7202362.1"
                     /translation="MYLALIGDIINSKQILERETFQQSFQQLMTELSDVYGEELISPF
                     TITAGDEFQALLKPSKKVFQIIDHIQLALKPVNVRFGLGTGNIITSINLNESIGADGP
                     AYWHARSAINHIHDKNDYGTVQVAICLDDEDQNLELTLNSLISAGDFIKSKWTTNHFQ
                     MLEHLILQDNYQEQFQHQKLAQLENIEPSALTKRLKASGLKIYLRTRTQAADLLVKSC
                     TQTKGGSYDF"
     gene            20910..>21510
                     /locus_tag="VBE22_000892"
     CDS             20910..>21510
                     /locus_tag="VBE22_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEO7202363.1"
                     /translation="MISDFLRDNPILTLLFCAHFLADFQWQSQSLADSKSHSWRGLWR
                     HLLIVFLPLAALMILIPETTLLNLSIWGSHIVIDSIKKLSYPWVEEGHFQKAAFIIDQ
                     LAHYTCIIVFYHALPTYLPPNHWLLPIKHFIVIALVFIIITKPINIVFKIFFNKFQAK
                     ELSSLLTQEKTKIMKEKSEDHEETIEGAGAMIGNLERLIM"
BASE COUNT         7334 a         3602 c         3998 g         6576 t
ORIGIN      
        1 ttgacagcaa gtaatcttaa ttgtataata taacagtata aagttagata aaagtgagat
       61 atttatgaga gagtctattt ttgaggaaac tgcaaaaggt aaagtatcaa ggcatgtcct
      121 tttaactata ttattggctt tgatattact atttacagca gatttcctta cctatattat
      181 tattgaattg attatctcgc ttgatttttg gaaactagag tgggatagcg atatcgtcca
      241 gttaatcatt ttactagcaa cttcaattag tacggtaatg ttatttatgt gggtgaaatt
      301 taaagagaag cgtccaatta gttctctagg gctatttaaa gaaggagccc taacagaatt
      361 atctagagga tggttagtcg ggagcgttct ctttggagcc tgcttaatat taacaataat
      421 ttcaggctct ggagttatcg aaactgttcg ttttacagct gataaattga tttggtttct
      481 tatttttgct gttggatggc aggtacaaag cgcatctgtt gaaatattgt gtcgtggatg
      541 gttacttccg gtgagtgcca caaagtatag taagatagtt tctgtcagta tcagttcaat
      601 ctttttcggt cttctgcata gcgctaataa tcatgtaagc cttatctcga ttttcaatct
      661 gtgtctcttt ggactatttc tatcgctcta tgttatttta aaaggtaaca tctggggggc
      721 gtgtggtatt catggcgcct ggaactgtgt acaagggagt gtctttggaa tcgaagttag
      781 tggagagcca atgttgtcta actccctagt ccatgttaaa acctatggtg ctgactggat
      841 tagcggtgga aaatttggtg tggaaggaag catgattacc agtattgttc ttatcgtagc
      901 atgttattgg ctttatcaaa aatcgaattt tccttacgac taatagattt aatctaaata
      961 agcttctctt actgaaagct tattttttat tttagtatta aaaaactatg ttttgtgata
     1021 taatatttta aagacgaaag gagtctattg tgccacaaaa tccaacaagt aacccagaga
     1081 aaaaaactta tttattatct gaattggtag actgctttaa aggtaaagct gtgccttcaa
     1141 aagcagaagc aggtgatatc cgtattatta atttgagtga tatgtctcct ttaggaattg
     1201 actaccataa cttaagaaca ttccaagatg aacaacgatc gcttttaaaa tatctacttc
     1261 aggaaggcga tgttttaatc gcttcaaaag gaactgtcaa gaaagttgct atttttgaag
     1321 agcaagatta ccctgtcgtt gcctctgcta atattacaat actaaggcct acacaacaca
     1381 ttagagggta ctacttaaag cttttttttg attcagaaga aggtcaacag gcacttgaga
     1441 atgctaacaa aggaaaagct gtgatgaata ttagcacaaa ggaactgtta aatattgcaa
     1501 taccaagtat cccgcttttt agacaggatt atcttatcca acgttataaa caggggctca
     1561 acgattacaa acgtaagata gcacgtgcag agcaggagtg ggaacgtatc caaaatgaca
     1621 tcagacaaca acttagctag aaccaaatac aaaacatcat aggaagagtt atcatgaacg
     1681 aaatcaaatg ccctcattgt ggaacagctt ttgccatcaa cgagtctgaa taccatcaat
     1741 tactagaaca aattcgtgga gatgcttttg acaaagaagt aagtgaacgg ttggaaaaag
     1801 aacgtctaat attaggggag caagcaaaaa atcaattaca ggaagttgtt gtagaaaaag
     1861 acaaggagat agctaaactt cagtacaaag tcaaacaatt tcttatagaa aaagacaatc
     1921 ttctcaaaga caatgagtac caactcgctg agcaattaaa tcaaaaagac atgatgcttc
     1981 gcgaccttga aaaccaaatc gatagactac gtttagagca tgaaaatagc ttgcaagagg
     2041 cgctaacaaa agtcgaacga gaaagagatg caatacaaaa tcagttgcac attcaagaaa
     2101 aagaaaaaga tttagcttta gcttcagtaa aaagtgatta tgaagtacaa ctaaaggcag
     2161 ccaatgaaca agtagaattc tataaaaact tcaaagctca acagtctact aaagcagtag
     2221 gagaaagttt agaacattat gctgaaacag aatttaataa agtgcgacat ttggcctttc
     2281 ctaatgctta ttttgagaag gacaatacat tatcaagtcg tggctcaaag ggggacttta
     2341 tctatcgaga aaaggatgaa aatgaccttg agtttttaag tatcatgttt gaaatgaaaa
     2401 atgagtctga tgatactatc aagaagcata aaaatgaaga ttttttcaaa gaattagata
     2461 aagatcgtcg tgaaaaatct tgcgaatacg cagttttagt aactatgctt gaagcagaca
     2521 atgactatta taatactgga attgttgatg ttagtcacaa ataccctaaa atgtacgtta
     2581 tacgtccaca attttttatc caattaattg gtattctaag aaatgcagca ctcaatacct
     2641 taaaatataa acaagagctt gctttgatga aagaacaaaa tattgacatc acacattttg
     2701 aagaagattt agatattttc aaaaatgcat ttgctaaaaa ttataattct gcaagcaaaa
     2761 atttccagaa agcaatcgat gaaatagata aatctattaa acgtatggga gctgttaagg
     2821 ctgctttaac aacgtctgaa aatcaactac gtcttgcaaa taataaatta gacgatgttt
     2881 ctgtcaagaa attaacaaga aaaaatccaa caatgaaagc aaaattcgat gctctaaaag
     2941 actaagaggt tatgattatg aagattagaa ctgctacact agacgatagt gaaaaattag
     3001 tccctttgta tcaagaatta ggatacgcaa tatcattatc agagattcaa tctattttaa
     3061 aggttattct tactcattca gattatggct ttttgatagc tgaggataat ggcaaactac
     3121 tcgccttcgt tggatatcat aaactttact tttttgaaaa aagtgggact tactatcgta
     3181 ttttagcact agtagttaat gaaaagcacc gtcgtaaagg catcgctagt caacttatta
     3241 atcacgttaa acagctagct aaaacagatg gaagtgaagt actggcttta aatagcagtt
     3301 taaaagaata tcgtcaggaa gcttatcatt tttatgaaaa cctagggttc aagaaggtta
     3361 gtacaggctt ttcatactat ttagattagg tataaatcct tatagcacta taccaaaaga
     3421 gcgtaagttt ttacgctcct ttctttgtgt taccgcttat ttactgatat aatataaaac
     3481 atgattacag gaataattaa tttaaagaaa gaagctggaa tgacatcaca cgatgctgta
     3541 ttcaagcttc gaaagatatt acatacaaaa aaaataggac acggcggaac tcttgatcca
     3601 gatgttgttg gggttttacc aattgcggtt ggaaaagcca ctcgtgttat tgaatatatg
     3661 acagaaagtg gcaaaattta tgaaggagaa atcactttag gatatgccac aagtacagaa
     3721 gattctagtg gagaggtcat ttcaagaact ccactaactc aatccgatct tagcgaggat
     3781 gttgtggatc atgcgatgaa aagttttacc ggtccaatta cacaagttcc tcctatgtat
     3841 tcagcagtta aagtaaatgg taaaaaattc tatgaatatg ctcgttcagg agaagaagta
     3901 gagcgaccta aacgacaaat aacaatttct gaatttagac gcacaagccc attgtacttc
     3961 gaaaaaggca tctgccgttt tagtttctat gtctcatgca gtaaaggtac ctatgttagg
     4021 acgttagctg ttgatctagg aataaaacta ggatatgcta gccatatgtc tttcctaaaa
     4081 agaacctcat ctgcaggatt atctattaca caatcattaa cgcttgaaga aattaatgaa
     4141 aaatataagc aagaagattt ttcattttta cttccaattg aatatggtgt tttagatctc
     4201 ccaaaagtta acctaactga ggaagataaa gttgaaatct catacggacg tcgtatctta
     4261 ttagaaaatg aagcagatac cttagcagct ttttatgaaa atagagttat cgctatacta
     4321 gaaaaacgtg gcaatgaatt taaaccacac aaagtattgt tataaagaaa gtcaatcatg
     4381 aaaatccatt acattaatga ttataaagat attcaagcta aagaagattg cgtcttagtt
     4441 ttgggctatt ttgatggctt acatctggga cataaagccc tctttgataa agctaaaaaa
     4501 atcgcgaccg aaaaaaattt gaaaattgtc gtcttaacgt ttaatgagac acctcgtcta
     4561 accttcgctc gcttccaacc tgaattattg ctacatctta cctctccaga aaaacgttct
     4621 gaaaaatttc aagagtatgg cgtcgatgag ttatatttaa tgaattttac cagtcatttt
     4681 tcaaaagtat cttctgacct ttttattaag aaatatattt atggtctcag agcaaaagct
     4741 gctgttgtcg gttttgatta taagttcggt cataatcgta ccagtggtga ttatctagcg
     4801 cgtaatttca agggaccagt ctatattatt gatgaaattt ctgaaggtgg tgaaaaaatc
     4861 tcttctacac gtattcgtca attgattaca gagggtaatg ttgagaaagc taaccaactt
     4921 ttgggttatg agttttcaac ctgtggcatg gtagtacatg gagatgctag aggacgaact
     4981 atagggttcc caactgctaa tctagctcct attaatcgca cttacttacc tgcagacggt
     5041 gtttatattt ccaatgttct gattaatgga aaatattata gagcaatgac gagcattgga
     5101 aaaaatatca cttttggtgg tacagaactt cgtttagaag ctaatatctt tgattttgat
     5161 ggcgacattt atggagaaac tattgaaatt ttctggttaa aaagaatcag agaaatggta
     5221 aagtttaatg gtattgacga tcttgtaaaa caacttaaaa aagataaaga aattgcttta
     5281 aattggaaaa aagatagtca aactctttaa ttttttgtat aatagtctct agtaaataca
     5341 agaggcggtt atatgattac cttattttta tcacccagct gtactagttg tcgaaaagcg
     5401 cgtgcttggc taagtaagca tgaggttgct tttgaagagc ataacattat tacaagtcca
     5461 ctcaataaag aagaactatt acaaattcta tcgtttacag aaaatggtac agaagacatt
     5521 atttccacac gttcaaaagt ctttcaaaaa cttgctattg atgtagacga attatcaacg
     5581 tcttcattaa tggaattaat ttcagaaaat ccaagtcttt tacgacgccc aattatttta
     5641 gacaaaaaac gtatgcagat tggttttaat gaggatgaaa ttcgtgcctt cttaccgcgt
     5701 gactatcgta aacaagaact gaaacaggcc actattcgtg ctgaaattga gggaaaacat
     5761 gattagtagt aattatagtt atcctcttga tccttcatgg aatacggaag atattactaa
     5821 ggtacttcgc tttttaaatc aagttgaaca cgcctatgaa aatagtgtta aagttgatga
     5881 tttgctagat agctataaag aatttaaaaa agttgttaaa tccaaggcgc aggaaaaaca
     5941 aattgaccgt gaattccaga gaactagcgg ttattccact tatcaagctg ttaaagcagc
     6001 acaacaaaaa gcgaaaggat ttatttcact tggacgctaa atttgatttc gctaaactat
     6061 tagtgtataa agctggtcaa ttcattaaat ctgagatgca aaatactttt gatattgagg
     6121 aaaagtctcg atttgatgat ttagtgacaa gtcttgataa aaaaacacaa aaattattaa
     6181 ttcaagaaat tattcaacat tacccagatg ataatatact tgccgaagaa gatgatgtgc
     6241 gctcacccat tgcacaaggt aatgtttggg ttttagaccc tattgatgga actgttaatt
     6301 ttattgttca aaaagataac tttgctgtta tgttagctta ttacgaagaa ggcgttggac
     6361 aatttggtat catctacgac gttatggctg atattcttta cagcggggga ggacattttg
     6421 atgtctatgc taacgataaa aaaattgttc cttttcaaga gtgtccttta gaacgttgct
     6481 tacttggagt aaattctgct atgtatgcag aaaatgattg cggtattgcc catttagctt
     6541 ctgaaacctt aggtgttcgt atctatggtg gtgctggaat tagcatggca aaagtcatgc
     6601 agggcaaatt attagcttat ttttcgtata ttcaaccttg ggattatgct gcagcaaaaa
     6661 tcatggggga gacattagga tttaccttat taacgcttga cggggaagaa cccaactatt
     6721 caacaagaca aaaagtaatg ttcctaccaa agtcgaaatt aaatcttatc caatcctacc
     6781 ttaccaaaaa ataaacttaa aaccttctaa ttaaatagaa ggtttttttg cacgcccgtg
     6841 attatgtagt ataatagaaa catgaaatta ccgaatgaat ttattgaaaa atatcagact
     6901 atacttaaag atgaagcaga agcttttttt gacagttttg aacaaaaacc catatcggca
     6961 tatcgcacca atcctctcaa agaaaagcaa cttgattttc caaatgccat cccaagtacc
     7021 ccttgggggc attatgggaa aatatcagga aaatcaatag aacacacaac tggccttgtt
     7081 tattctcaag aacctgctgc tcagatagtg gctcagattg ctgaaccaca agaaggaatg
     7141 aaagtgctag atttagcagc tgcacctggt ggaaaaacaa cacaccttct atcatattta
     7201 aacaatactg gtctattagt tagtaatgaa atttcaaata aacgtagtaa aattttagtc
     7261 gaaaatgtcg aacgttttgg tgctagaaat gttatcgtga ctaatgaaag ctcccaacga
     7321 ctagccaaat gttttaattc tttttttgat cttattgttt ttgacggtcc gtgttctggt
     7381 gagggaatgt tccgcaaaga tcctcaagca atacaatatt ggcacaaaga ttaccccact
     7441 gaatgtgcac agttacaaag agatatttta aaagaagcaa tcaaaatgct agctcatggt
     7501 ggtatacttg tatactctac ttgtacatgg tcaccagaag agaacgaaga agtggttaat
     7561 tggctccttc aagagtatga ttacttagaa cttgttgata ttcctaaact aaatgggatg
     7621 gttgaaggga ttaatgtacc acaagttgca aggatgtacc ctcatcattt tcaaggtgaa
     7681 ggacaattcg ttgcaaaact gagagatact agatctaagg aagcacagaa aattaagcca
     7741 aaagcacaga aaataaataa aatgcagtta caattgtggc aacaatttgc acaagaccat
     7801 ttaaagatag acttgaacgg tgttttagat gttttcggcg accaactcta tcttctacct
     7861 aatggtctcc ccgatttgtc taaattaaaa atagcacgca atggtctcca tctaggcact
     7921 ttcaagaaga atcgttttga gccatcgttt gctttaggaa tggcacttag cgaacatgac
     7981 ctagtacagt ctattgaaat tgatatagaa cagtttgagg tgtacgtatc tggaaatgta
     8041 gtcaaactag acaagactgt tccaaacggt tggtatcaaa ttcttgtaaa aggcaatgga
     8101 ttaggttttg caaaagtgac aaataatact cttaaaaatt attaccctaa aggactacga
     8161 tttcagacat aactgttatt gaatactttt aataaaaaag gaaaaagaaa tacttttcaa
     8221 accctagaaa cttatggtac tatgggacag tgatgtgtgt ttttactatt tgtaaatcat
     8281 taaacactca atgattttag taatatatat caactactaa atatcaaaat ttattcaaaa
     8341 gaataggaaa atgaaaatga aaaaaaacat ttttttgtta accttgctag ccaccgtctt
     8401 attgacgctg tctggttgtg ctaattggat tgataaaggt caatcaatca ccagcgttgg
     8461 ttcaaccgcc ttacagccac ttgtagaagc tgcagcagat gagtttggta agacaaacct
     8521 cggtaagact atcaatgtcc aaggaggagg ctcaggaaca ggcttatcac aagtacagtc
     8581 tggagctgtt caaattggta atagcgactt atttgcagaa gaaaaagatg gtattgatgc
     8641 taagaaattg aaagaccatc aagtcgcagt agctggatta gctgttattg ttaataaaaa
     8701 agttaatgtt aaaaatctaa ccacccacca acttcgtgat atttttgcag gaaaaatcaa
     8761 gaattggaaa gaagtgggag gacaagattt agatatttcc atcatcaatc gagcagcaag
     8821 ttctggatcc cgtgcaacct tcgataatac tattatggga aatgttgctc ctattcaaag
     8881 ccaagaacaa gattcaaatg gtatggtgaa atcaattgtt tctcaaacac ctggtgcgat
     8941 ttcatacctc gcttttgcat acgttgataa atctgtcgga acattaaaac taaatggttt
     9001 tgcgccaaca gctaaaaatg ttactaccga caactggaaa ctttggtcat atgaacatat
     9061 gtatacaaaa ggtaatgaaa caggtctaac aaaagaattc ttagactaca tgaaaagtga
     9121 taaagttcaa agtagtatcg tacaacatat gggatatatc tctattaacg acatgaaagt
     9181 tgttaaagat gctgaaggta aagtaacccc aaaataaaac ttaatcacat ctagataaag
     9241 catcttgatc ctaggaggaa ttatgaaaaa tcaagaatta gctaaaaaat taacatctcc
     9301 ttcaaaaaac tctcgattag aaaaatttgg taaaggaata acttttcttt cgcttgccct
     9361 aatcgtcttc attgttgcga tgattttgat ttttgttgct caaaaaggac tatctacatt
     9421 ttttgttgat ggcgttaaat taacagactt cttatttaat acaaaatggg aaccaagtgc
     9481 taagtcattt ggtgcttttc caatgatagc tggatcattt attgttacta ttctatcagc
     9541 tatcattgct acaccatttg ctatcggtgc tgccgtattt atgactgaaa tctcacctaa
     9601 atatggttct aaaattttac aacctgcagt tgaattacta gttgggattc cttctgttgt
     9661 ttacggtttt attggtttac agattattgt tcccttcgtt cgctctattt ttgggggaac
     9721 aggttttggc attttatctg gggtctgtgt cctcttcgtc atgattctac caactgttac
     9781 tttcatgaca gtagatagcc ttagagctgt gccacgtcac tataaagaag caagtttagc
     9841 catgggggca actcgttggc aaacaatttg gcgtgttata ttaaacgctg cacgtccagg
     9901 aattttcact gcaattgttt ttggtatggc acgtgcattt ggtgaagctc tagcaattca
     9961 gatggttgtc ggaaactctg caattttacc aacgtcattg acaacaccag cagcaacatt
    10021 aacgtctgtt ctaacaatgg gtattggtaa tactgtaatg ggaactgtac aaaacaatgt
    10081 cctctggtcg ctagccttag tacttttaat tatgagttta gcatttaata cagttattaa
    10141 gttaattaca agggaaggaa agaaaaacta tgaacgctaa aaaggctgac aaattagcaa
    10201 ctacaatact ctatagtatt gcagctatca tcgttacaat cctcgcctct cttttaattt
    10261 ttattcttgt tcgcggattg ccacacgtat cttggtcatt tttaactggg aaatcatcat
    10321 catatgaagc aggaggcggt attggaatac aattatacaa ttcctttttc ctacttattg
    10381 ttactctgat tatttctatt cctctatctt taggagctgg aatatacctc tcagaatatg
    10441 ctaaaaaagg acgtttaaca aactttgtta gaacatgtat cgaaattttg tcatctcttc
    10501 cttctgttgt agttggtctg tttggttacc taatcttcgt tgttcaattt caatatggct
    10561 tctcaattat ctcaggtgcc ttagcactta ctgtttttaa cttaccccaa atgacacgta
    10621 gtgttgaaga tagccttcaa aatgtacacc acacacagcg tgaagcagga ttggccctag
    10681 gtatttctcg ttgggaaacg gtcatttacg tcgttgtccc tgaagctctg ccaagtattg
    10741 taactggtgt tgtgctagct tcaggacgta ttttcggtga ggcagcagca cttatttata
    10801 cagctggtca atctgctcca gcacttgatt ggtcaaattg gaacgtcctt agtgttacaa
    10861 gtccaatctc aattttccgt caagcagaaa ctttagctgt tcatatttgg aaagttaata
    10921 gcgaagggac aatccctgat gcaacacaag tatctgccgg tagcgccgca gtcttattgg
    10981 ttgttattct tattttcaac ctttcagctc gctcgatcgg taagaaactt cattcgaaat
    11041 taacctcgtc taattaaaag gagtagtaat ggcagaatat aattgggacg aacgccatat
    11101 cattaccttc cctgaagaaa attcagctct caccacaaaa gatttacacg tttactacgg
    11161 tgagaaagaa gctatcaagg gaattgatat gcaatttgaa aaaaataaaa ttacagcctt
    11221 aatcggccca tctggatgtg gaaaatcaac atatcttcgt agcttaaatc gtatgaacga
    11281 tacgattgat attgcacgcg tgactggaca aatcatgtat gaaggaattg atgttaacgc
    11341 tcaagatatc aatgtttacg agatgcgaaa acatatcgga atggttttcc aacgtccaaa
    11401 cccgtttgca aaatcaattt ataaaaacat tacctttgca tatgagcgtg ctggtgtaaa
    11461 agataaaaaa ttcttagatg aagttgtcga aacctcacta aaacaagctg ctctatggga
    11521 tcaagtaaaa gatgaccttc ataaatcagc attcacttta tctggcggtc aacagcagcg
    11581 attatgtatt gcacgtgcta tcgcagttaa accagaaatt ctcttaatgg atgaacctgc
    11641 ttcagcactc gatccgattg caactatgca actagaagag acaatgtttg aactcaagaa
    11701 aaattacact ataattatcg taacacataa tatgcaacaa gcagcacgtg ctagtgatta
    11761 tactgcattt ttctatcttg gagacttaat cgaatacgat aaaacgaata acattttcca
    11821 aaatgctaaa tgtcaatcta ctagcgatta tgtttctggt cgatttggat agtgaggtaa
    11881 aagatgacac aacctatttt acaagtcagt gatttgtctg tttactataa taagaaaaaa
    11941 gctttaaaag aagtctcaat ggacttctat cctaatgaaa ttacagcctt aattggtccg
    12001 tctggttctg gaaagtcaac actcttacga gctattaacc gtatgggaga cttaaatcct
    12061 gaagtcactc taacaggagc agtgatgtat aacggacata atgtttatag cccgcgcact
    12121 gataccgtag aacttcgtaa agaaattggg atggtttttc agcagcctaa tccatttcct
    12181 atgtcagtat ttgaaaatgt tgtttatggt cttagactca aaggaataaa agacaaagct
    12241 acgttagatg aagccgtaga gacttcattg aagggcgcgt ctatctggga cgaggtcaaa
    12301 gatagattac acgactctgc tcttggttta tctggtggtc aacaacaacg tgtttgtatt
    12361 gccagaaccc ttgctaccaa gcctaaaatt atccttctag atgagccaac ctctgcactt
    12421 gacccaattt ctgctggtaa aattgaagag actttacatg gtctaaaaga tcaatatacc
    12481 atgttacttg ttacacgttc tatgcagcaa gcctcacgta tttctgatcg tacaggtttc
    12541 ttcttagacg gtaacttaat tgaatatggc aatacaaaag aaatgtttat gaatcctaaa
    12601 cataaagaaa ctgaagatta cattacaggt aaattcggtt aataccatat aataagatca
    12661 gaaagagaat acaatatgtt aagatcaaaa tttgatgaag aattagataa actgcataac
    12721 caattctatg caatgggaat tgaagctatc ggacaaatca aaaaaacagt tcgtgctttt
    12781 gtcagtcatg atcgagagtt agctaaagaa gtgatagaag atgatgtaac gcttaacaac
    12841 tttgaaacga agttagagaa aaaatcattg gaaattattg cactccagca gcctgtatca
    12901 caagacttac gcaccgttat taccgtttta aaagctacta gtgatgttga gcgtatggga
    12961 gaccatgcag cagcagttgc aaaagcaacc atccgtatga agggcgagga aagaatccca
    13021 gcagtagaat tagaaatcaa taatatggga aaagcagtta aaaacatgct tgaagaagcc
    13081 ttaacagctt atatcaatgg tgatgatgaa aaagcttacg aagtagcagc aatggatgaa
    13141 atagttgatg attacttccg tgatatccaa aaaatggtag ttgaaacaat tcaaaagcat
    13201 cctgacgtag catttgccgc caaagaatac ttccaagtat tgatgcactt ggagcgaatt
    13261 ggagattatg gcaaaaatat ctgtgaatgg attgtttatt taaaaactgg aaaaattatc
    13321 gagttatgat ataattattg cttgtagagt ggtaattgta agtatagttc gtgcgataga
    13381 aaagctgtaa ctatcttgtt acagcttttc tatcttttct agcccataca ggactagtaa
    13441 tgtttcaaat gtttctgaat tagttaggag ttatatgaaa actgtagaac attttgttac
    13501 caaatttgtc cctgaaaact ataacctttt cttggatatt aatcgtcaaa ctaaaacctt
    13561 ttcaggtaac gttgctgtgt ctggcgaagc cttagataac aatatctctt ttcaccaaaa
    13621 agggttaacc attaaatctg tcttattaga taatcaacca ttagatttcc aactagatga
    13681 agacaatgaa gctatgcata ttcaattgca tgaaactggt agcatggtat tagtctttga
    13741 attcagcggt cacattaccg ataatatgac aggtatgtat ccatcttact atacagttaa
    13801 tggcatcaaa aaagaggtta tctccacaca atttgaaagt cattttgctc gtgaagtatt
    13861 cccaagtatt gacgaaccag aagcaaaagc aacctttgat ttgagcctaa aattcgatca
    13921 aaaagaagga gagattgctc tttcaaatat gccggaaatc aatgctgaac aacgtcaaga
    13981 aacaggttta tggacatttg acaccacacc taaaatgtcc tcatatttat tagcttttgc
    14041 cttgggggaa ttgcatggta aaacaacaca taccaaaaac ggcactcttg ttggctcata
    14101 tgccacaaaa gcccatcagt taaatgaatt agatttttct ttagatatcg ttgtgcgtgt
    14161 tattgagttt tatgaggact actttggtgt acgttatcct attcctcagt cactccacgt
    14221 tgctctacct gatttttctg ctggagctat ggaaaattgg ggattagtaa catatcgtga
    14281 agtctatctt ttagtagatg agaactctag tgtttccagc cgtcagcaag tagcattagt
    14341 tgtagcacat gaaatagctc accagtggtt tggtaacctc gttactatga aatggtggga
    14401 tgatttatgg ttaaatgaat catttgctaa tatgatggaa tatgtatcaa ttgattatat
    14461 tgaaccaaaa cttaatatat ttgaagactt tcaaacaggt ggtctaccat tagctcttaa
    14521 gcgtgatgcc acagatggcg tccaatctgt tcacgttgag gtcaatcatc ctgatgaaat
    14581 taacacactt tttgatccag ctatcgttta tgcaaaaggt agccgcctca tgcacatgtt
    14641 acgtcgctgg ttaggcgata cagattttgc cgcaggacta aaaatttatt ttgaaaaaca
    14701 tcaatatcaa aatactattg gtcgtgacct ttggaatgca ctttctcaaa cctcaggaaa
    14761 agatgttgca gctttcatgg attcttggtt agagcaacca ggataccctg tgatggcagc
    14821 caaaatagaa gaagatgagc tcattttaac tcaaaaacaa ttctttattg gtgaacacga
    14881 agataagtct cgcttgtggc aaattcctct taatagtaat tgggaaggaa tcccagagat
    14941 attaaccgag gaaacggtag taatccccaa ttttagtcag ttagctgaaa agaacaaaga
    15001 aaatggtgca ctgcgtttca atactgaaaa tactgcacat tacattacta actatcaagg
    15061 tcaactctta gagcatatca tttctgatct tcctcttatg gataacattt ctaaactaca
    15121 aattgtacaa gaacgtcatt tacttgctga aagtggtatg atttcatata gtagtttaat
    15181 acctttagtc tctcttttga gccaagagac atcatatctt gttaactctg ctattaagag
    15241 tgttattgat ggtttatcct tattcgtcca ggaagatagc caagatgaat tcgactttaa
    15301 agaatttgtt aataaattat cagcatttaa ttttaataga cttggctttg aaaaaagaga
    15361 aggagaagga gatgatagcg aaatggtccg tcatctatcc ctaagccttg ctctctactc
    15421 agataatgaa catgctattg aagaagctca ccatattttt aaagcccacg aaaataatat
    15481 cgctgctata ccagctgcaa ttcgcctgtt agttcttacc aatgaaatga agcatttcga
    15541 atccaaagaa cttagccact tgttattaga gacttatagt actactaccg atgggaattt
    15601 caaacgacaa ctagcgagtg ctttatctca tactacagat agcaagacac tcaagaaatt
    15661 attgtcagat tggaaaaata aagatattgt gaaaccacaa gacttagcta tgagttggta
    15721 tgctactttc cttaaaaatt cttttacaca acaaagtgtc tgggagtggg cccaagaaaa
    15781 ctgggaatgg atcaaagcaa ctctaggtgg tgatatgagt tttgataaat ttgtgattta
    15841 tccctcaagt agtttcaaaa cagaagaacg ccttgaacaa tataaaaact ttttcgagcc
    15901 tcaattaagc gatatggcga ttagccgtaa tatttcaatg ggaattaaag aaatttctgc
    15961 tcgtgtcctt ttaatcacga agcaaaaaga agaagttatc aatactatca aaaaatatat
    16021 ctaataaact caaatagaga ttgatggatt attcaatctc tatttctttg tctgtgtcat
    16081 gaaattagct gacataaaac atactttatt taggatttat taagcaaagt ttggtattct
    16141 aatattaata gttcagataa aatatgtaca tcgaaaggat atactatgat aaaaatttta
    16201 ttaattgaag atgacttgag tctttccaat tccgtttttg atttccttga tgactttgcc
    16261 gatgtcatgc aaatttttga tggagaagag ggtctttacg aagccgaaag tggagtctat
    16321 gatctgattc ttctagatct catgttacct gaaaaaaacg gcttccaagt gttgaaagag
    16381 ctacgtgaaa aaggtattac aacccctgta ttaataatga ccgctaaaga aagcattgat
    16441 gataagggac aaggtttcga cttaggtgct gatgattatc taacaaaacc tttttaccta
    16501 gaagaactaa aaatgcgaat tcaggctctt ttaaagcgtt ctggaaaatt taatgataac
    16561 agcctaattt atggtgatat tcgcgtagac atgtctacta actcaacctt tgttaatcaa
    16621 acagaagttg agctcttagg taaagagttt gatcttttag tctatttcct tcaaaatcaa
    16681 aatgttatct taccgaaatc tcaaatcttt gatcgtattt ggggatttga tagtgataca
    16741 acgatttcag ttgtagaagt ctatgtctct aaggttcgga aaaaattaaa aggaacactt
    16801 ttttcagaaa accttcaaac tttgcgtagt gtaggatata ttttaaagca tgtcgaaacc
    16861 gattaagata agtaaattca aaaaaaatgt ctcagatagt cattttatcc attttttcac
    16921 agtttttagt ggtatttttc tggttatgac tgttatcatc ctccaagtca tgcgctatgg
    16981 tgtttattct tctgttgata gtagtttaaa gtatatcagt acccatccta aaaattacat
    17041 caatatggtt atgtcacgta ctgctgccta cctagataac tccaatatcg caagtgtcaa
    17101 gttaaaaccc ggcggtcaga cagtagctaa tactgatatt attcttttta catcagaaga
    17161 agaagtcatt aactattttg acgcattttc aaactatcag tttttaaaac ctaataaaaa
    17221 aaatctaggt ggcattagtg aattaacttt aaccaacatc tttggtcaag atgagacata
    17281 tcatgctgtc accgtaaaag ttaataaccc tgcttatcct aatgtgactt acatgacagc
    17341 tattgttaat attgatcaat tagtaaatgc taaagaacgc tatgaaaaaa ttattatatt
    17401 tgtcatgact actttttgga ttatttcaat tggagcaagt atctacttag ctaaatgggc
    17461 acaaaaaccg attatagaaa actacgaaag acagaaggct tttgtcgaaa atgctagcca
    17521 cgagctgcgt actcccctag ctgtcttaca aaatcgccta gaaacgctat tcagaaaacc
    17581 gaatgccacc attttagaaa atagtgaaaa tatcgcttca agtctagatg aagtacgaaa
    17641 tatgcgtatt ttaaccacta atcttttaaa cttagcaaga cgtgatgatg gaatcaaacc
    17701 agaattagct gttattaagc ctacactttt tgatagtatc tttgagaatt atgatctaat
    17761 cgcccaagag aatggtaaaa attttactgg acataatatg atccaagata gtttcaaaac
    17821 agataagaca ctgctaaaac aactcatgac tattttattt gataatgcta taaaatatac
    17881 tgacaatgat ggtagcattg actttaccat atctgaaact gataagtatc tctttttaga
    17941 aatcgctgat aatggaccag gaatttcaga ggaggataaa gtcagaattt ttgatcgttt
    18001 ttaccgtgtt gacaaagcaa gaacacgaca acaaggaggg tttggcctcg gcctttcttt
    18061 agctcaacaa atcgttaact cactgcgagg taatattaca gtaattgata ataaacctag
    18121 aggcagtatt tttaagataa aactaccaaa agtaaaaggt taggccatgt tagtcctaac
    18181 cttttaaatt ttaaagtggt cttgcatact tgatatagca agctgatagg aaatttggta
    18241 taataaaagc tatggaaatt gaaaaaacaa atcgtatgaa tgcccttttt gagttttatg
    18301 cggcattatt aacagataaa caaatgaatt atatcgagtt atactacgcc gatgactaca
    18361 gccttgctga aatagctgaa gaatccggtg ttagccgaca agctgtctat gataatatca
    18421 aacgaactga aaaaattctt gaggcgtacg aaatgaaact acatatgtat tcagattata
    18481 ttgtccgtag tcagattttt gatgacattc tagaaaaata taccgatgat gcctttttac
    18541 aagaaaaaat ttccattcta agtagcattg ataatagaga ttaaatgagg agaaataatt
    18601 tatggctttc gaaagtttaa cagagcgcct gcaaggagtt tttaaaaata ttcgtggtaa
    18661 gaaaaagtta tctgaaaaag atgtgcaaga agtcaccaag gagattcgtc tagctctttt
    18721 agaagctgac gttgctcttc cagttgttaa aacctttatt aaacatgttc gtgaacgtgc
    18781 tgttggacat gaaatcattg atacactaga tcctacgcaa caaattgtta agattgtcaa
    18841 tgaagagcta acagatttat taggggctga gacctctgaa attgaaaaat cccctaaaat
    18901 tccaaccatc attatgatgg ttggtctaca aggtgctggt aaaacaactt ttgccggtaa
    18961 gttagcaaac aaactaatca aagaagacaa tgctcgtcct atgatgatag cagctgatat
    19021 ttatcgacca gctgctattg atcagttaaa aacccttggt agccagatta atgttcctgt
    19081 ctttgatatg ggaactaacc attcagcagt tgagattgtc actaaaggtt tagaacaggc
    19141 tcgtgaaaat cgtaatgact atgttcttat tgatacagcc ggacgcctac aaattgatgc
    19201 aacccttatg caggaattgc atgatgtcaa agccatagct caacctaatg aaatactcct
    19261 tgtcgttgac agcatgattg gacaggaagc tgcaaatgtc gctgaagaat ttaaccgtca
    19321 gctcagcatt agtggtgttg ttctgacaaa gattgacggt gatacacgcg gtggtgctgc
    19381 cctttcagta cgcgaaatta ctggaaaacc aattaaattt acagggacag gggaaaaaat
    19441 cactgatatt gaaactttcc atcccgatcg tatggcaagc cgtatattgg ggatgggaga
    19501 cctgctaaca cttattgagc gcgctagtca agaatacgac gaaaaacgtt caatggaatt
    19561 agcggagaaa atgcgtgaaa acacctttga cttcaacgat tttattgatc aattagatca
    19621 agttcaaaat atggggccaa tggaagatct ccttaaaatg cttccaggaa tggctaacaa
    19681 tccagcaatg aaaaacttca aagtggatga aaacgaaatt gctcgcaaaa gagccattgt
    19741 gtcatcaatg actccagaag agagagaaaa tccagatctc ctcaatccaa gtcgtagacg
    19801 tcgtattgct gctggatcag gtaatacttt cgttgatgtt aataaattca ttaaagattt
    19861 taaccaggca aaacaaatga tgcaaggcgt gatgtccggt gatatgaata aaatgatgaa
    19921 aaaaatggga attgatccta ataacctccc taaagatatg cctggaatgg acggaatgga
    19981 tatgtctaat ttagaaggta tgatggggca aaatggcatg cctgacttat ctagtttagg
    20041 tggcgatatg gacttcagcc aaatgtttgg tggtggctta aaaggaaaag taggagcctt
    20101 tgcagctaaa caatcaatga aacgtatggc taataaaatg aagaaagcta agaagaaacg
    20161 caaataaaaa tgtctattta aatagacatt tttatttttg tctgctataa tagacaaaaa
    20221 ggtggtgata tttatgtatt tagcattaat cggtgatatc attaattcaa aacagatact
    20281 tgaacgtgaa actttccaac agtcttttca gcaactaatg accgaactat ctgatgtata
    20341 tggtgaagag ctgatttctc cattcactat tacagctggt gatgaatttc aagctttatt
    20401 gaaaccatca aaaaaggtat ttcaaattat tgaccatatt caactagctc taaaacctgt
    20461 taatgtaagg ttcggcctcg gtacaggaaa cattataaca tccatcaatt taaatgaaag
    20521 tatcggtgct gatggtcctg cctactggca tgctcgctca gctattaatc atatacatga
    20581 taaaaatgat tatggaacag ttcaagtagc tatttgcctt gatgatgaag accaaaacct
    20641 tgaattaaca ctaaatagtc tcatttcagc tggtgatttt atcaagtcaa aatggactac
    20701 aaaccatttt caaatgcttg agcacttaat acttcaagat aattatcaag aacaatttca
    20761 acatcaaaag ttagcccaac tggaaaatat tgaacctagt gcgctgacta aacgccttaa
    20821 agcaagcggt ctgaagattt acttaagaac gagaacacag gcagccgatc tattagttaa
    20881 aagttgcact caaactaaag ggggaagcta tgatttctga ttttttaagg gataatccaa
    20941 tcctaacttt attattttgt gcacattttt tagctgactt tcagtggcaa agtcaatcct
    21001 tagcagatag taaatcacat agttggaggg gactctggag acatctcttg attgttttcc
    21061 tccccctagc agctttaatg atattgatac cagaaactac tttgttaaat ttaagcatat
    21121 ggggaagtca tattgttatt gatagtataa agaaattgag ttacccttgg gtagaggaag
    21181 ggcatttcca aaaagcagct tttattatag accaactagc ccactatact tgcattatcg
    21241 ttttttacca tgcgcttcct acatatttac cgcctaatca ttggcttttg cctatcaagc
    21301 actttattgt catagctctg gtttttatca ttatcaccaa acccattaat attgtgttta
    21361 aaattttctt taataaattt caagctaaag aattatcatc attattaaca caagaaaaaa
    21421 caaaaataat gaaagaaaaa tctgaagatc atgaagaaac aattgaagga gcaggagcta
    21481 tgattggtaa tttagagcgt ctaattatgg
//
LOCUS       DATRTN010000022        20451 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.022, whole genome shotgun sequence.
ACCESSION   DATRTN010000022 DATRTN010000000
VERSION     DATRTN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20451
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.022"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            9..2270
                     /locus_tag="VBE22_000893"
     CDS             9..2270
                     /locus_tag="VBE22_000893"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEO7202364.1"
                     /translation="MPTKPNEAFEELKGLGFPLNKKNSDKANLEAFLRHSFLNQTDTD
                     YALSLLIVDAKTDALTFFKSNSDLTLENLQWIYLQLLGFVPFVDFKDPKAFLQDINFP
                     VSYDNIFQSLHHLLACRGKSGNTLIDQLVADGLLHADNHYHFFNGKSLATFNTNQLIR
                     EVVYVEISLDTMSSGEHDLVKVNIIRPTTEHTIPTMMTASPYHQGINDPAADQKTYQM
                     EGALAVKQPKHIQVDTKPFKEEVKHPSKLPISPATESFTHIDSYSLNDYFLSRGFANI
                     YVSGVGTAGSTGFMTSGDYQQIQSFKAVIDWLNGKVTAFTSHKRDKQVKADWSNGLVA
                     TTGKSYLGTMSTGLATTGVEGLKVIIAEAAISTWYDYYRENGLVCSPGGYPGEDLDVL
                     TELTYSRNLLAGDYIKNNDCYQALLNEQSKAIDRQSGDYNQYWHDRNYLTHVNNVKSR
                     VVYTHGLQDWNVKPRHVYKVFNALPQTIKKHLFLHQGQHVYMHNWQSIDFRESMNALL
                     SQELLGIDNHFQLEEVIWQDNTTEQTWQVLDAFGGNHQEQIGLGDSKKLIDNHYDKEA
                     FDTYCKDFNVFKNDLFKGNNKTNQITINLPLKKNYLLNGQCKLHLRVKTSDKKAILSA
                     QILDYGPKKRFKDTPTIKFLNSLDNGKNFAREALRELPFTKDHYRVISKGVLNLQNRT
                     DLLTIEAIEPEQWFDIEFSLQPSIYQLSKGDNLRIILYTTDFEHTIRDNASYSITVDL
                     SQSYLTIPTNQGN"
     gene            2274..2633
                     /locus_tag="VBE22_000894"
     CDS             2274..2633
                     /locus_tag="VBE22_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202365.1"
                     /translation="MKLLTKERFDDSQHFWYQINLLQESNFGAVFDHDNKNIPQVVAT
                     IVDDLQGSGSSNHFWYFGNTTDTSILMIAHLNRKFYIQVNLKDFDFALNLIAINNWKS
                     LLQTQLEALNDTLAIFQ"
     gene            2679..3659
                     /locus_tag="VBE22_000895"
     CDS             2679..3659
                     /locus_tag="VBE22_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO7202366.1"
                     /translation="MSSYWNNYPELKKNIDETNQLIQERIQVRNKDIEAALSQLTAAG
                     GKQLRPAFFYLFSQLGNKENQDTQQLKKIAASLEILHVATLIHDDVIDDSPLRRGNMT
                     IQSKFGKDIAVYTGDLLFTVFFDLILESMADTPFMRINAKSMRKILMGELDQMHLRYN
                     QQQGIHHYLRAISGKTAELFKLASKEGAYFGGAEKEVVRLAGHIGFNIGMTFQILDDI
                     LDYTADKKTFNKPVLEDLTQGVYSLPLLLAIEENPDIFKPILDKKTDMATEDMEKIAY
                     LVVSHRGVDKARHLARKFTEKAISDINKLPQNSAKKQLLQLTNYLLKRKI"
     gene            complement(3744..5492)
                     /gene="cydC"
                     /locus_tag="VBE22_000896"
     CDS             complement(3744..5492)
                     /gene="cydC"
                     /locus_tag="VBE22_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEO7202367.1"
                     /translation="MFKIPLFKELKTDQWVKPFFKQYKVSLVIALFLGFMTFFSASAL
                     MFNSGYLISKSASLPSNILLVYVPIVLTRAFGIGRPVFRYIERLTSHNWVLRMTSQLR
                     LKLYHSLESNAIFMKRDFRLGDVMGLLAEDINYLQNLYLRTIFPTIIAWLLYSFIIIA
                     TGFFSLWFALMMLLYLAIMIFLFPLWSILANGARQTREKELKNHLYTDLTDNVLGISD
                     WIFSQRGQEYVALHERSESELMAVQKKIRSFNNRRALIVELVFGFLAILVIIWASNQF
                     IGHRGGEANWIAAFVLTVFPLSEAFAGLSAAAQETNKYSDSIHRLNELSETYFETTQN
                     QLPNKPYDFSVKNLSFQYKPQEKWVLHHLDLDIKEGEKIAILGRSGSGKSTLASLLRG
                     DLKASQGKITLGGADVSIVGDCISNYIGVIQQAPYLFNTTLLNNIRIGNQDASEEDVW
                     KVLERVGLKEMVTDLSDGLYTMVDEAGLRFSGGERHRIALARILLKDVPIVILDEPTV
                     GLDPITEQALLRVFMKELEGKTLVWITHHLKGIEHADRILFIENGQLELEGSPQELSQ
                     SSQRYRQLKAADDGDL"
     gene            complement(5485..7203)
                     /gene="cydD"
                     /locus_tag="VBE22_000897"
     CDS             complement(5485..7203)
                     /gene="cydD"
                     /locus_tag="VBE22_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEO7202368.1"
                     /translation="MLDKAVMRLSGIHKLLGLLAGLDVLQAIFIIGQAYYLSLSITGL
                     WEGQKLSSQTVYILLFMVSYLGRHVIDYIKNRKLDDFSTAQSSLLRRQLLDKLFDLGP
                     KVVQEQGTGNVVTMALDGVSLVENYLRLVLNKMINMSIIPWIILAYIFYLDIESGAIL
                     LIVFPLIIIFMIILGYAAQAKADKQYESYQVLSNHFLDSLRGIDTLKYFGLSKRYGKS
                     IYQTSESFRKATMSTLKIGILSTFALDFFTTLSIAIVAVFLGLRLLNEQIYLLPALTI
                     LILSPEYFLPVRDFSSDYHATLDGKNAFQAIQKVLNKTGIKGEQLVIDDWSKESHLDL
                     ENIAIAYDQKRVVEDVTLRFRGHQKVALVGVSGSGKSSLINLLSGFLGPDKGSLKVDG
                     REVVNLDQEDWHKQMIYIPQTPYVFEMSLRDNITFYTPNASDEEVIRAIHMVGLDSLL
                     SELPDGLETRIGNGARPLSGGQAQRIALARAFLDQNRRIMVFDEPTAHLDIETELELK
                     EKMLPLMSDRLVIFATHRLHWLNQMDVIVVMEKGRVVEVGSYQELLAKKGYLYQLKHA
                     MGGNNV"
     gene            complement(7203..8222)
                     /gene="cydB"
                     /locus_tag="VBE22_000898"
     CDS             complement(7203..8222)
                     /gene="cydB"
                     /locus_tag="VBE22_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEO7202369.1"
                     /translation="MSALQFFWFFLIGLLFSGFFFLEGFDFGVGMAVQTLTHNEHEKD
                     QVVETIGPVWDGNEVWLLTGGGAMFASFPYWYASLFSGYYLILLTILFGLIIRGVSFE
                     FRHKVPAEKKQFWNWTLTIGSAIVPFFFGIMFISLIQGMPLDASGNLSAQFSDYFNIF
                     SLVGGVAMVLLAYLHGLNYIALKTEGPIRERARNYAQLLYWVLYLGLALFAVLLYFKT
                     DFFSNHPIVTTIMVLVIVVLAVLAHASTFKGAEMTAFLASGLSLVSVVVLLFQGLFPR
                     VMISSISPKYDLLIQNASSTPYTLKVMSIVAITLVPFVLAYTAWAYYIFRKRITLPVI
                     VTGEK"
     gene            complement(8223..9650)
                     /locus_tag="VBE22_000899"
     CDS             complement(8223..9650)
                     /locus_tag="VBE22_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEO7202370.1"
                     /translation="MTIETLARFQFAMTTVFHFFFVPFTIGTCLVVAIMETMYVITKN
                     EEYKKLTKFWGNIMLLSFAVGVVTGIIQEFQFGMNWSDYSRFVGDIFGAPLAIEALLA
                     FFMESTFLGLWMFTWDNKKISKKLHVTFIWLVVFGSLMSAMWILTANSFMQHPVGYEV
                     VNGRAQMTDFLALVKNPQFFYEFTHVIFGAITMGGTVVAGMSAFRLLKSEQLKDTTVE
                     LYKKSVRIGLVVALLGSISVMGVGDLQMKALIHDQPMKFAAMEGDYEDSGDPAAWSVV
                     AWANEAEHKQVFGIKIPYMLSILSYGKPSGSVKGMDTANKELVAKYGKDNYYPMVNLL
                     FYGFRTMAAMGTAIMGVSVLGLFLTRKKKPILYKHKWMLWIVALTTFAPFLANTFGWI
                     VTEQGRYPWTVYGLFKIKDSVSPNVSVASLFVSNTVYFLLFGGLAVMMISLTIRELKK
                     GPEYEDEHGHHGAYTSIDPFEKGAY"
     gene            complement(9753..10961)
                     /locus_tag="VBE22_000900"
     CDS             complement(9753..10961)
                     /locus_tag="VBE22_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7202371.1"
                     /translation="MKEILVLGAGYAGLKAVRNLQKQSGDFHITLVDMNDYHYEATEL
                     HEVAAGSQPKEKITFPIKDVINTNKVNFMQDEVLKVDAENKTVTVKNNGELHYDYVVV
                     ALGFVSETFGIKGAMENALQMTNISQAENIHNHIVNTMKLYRETKDENLLKLLVCGAG
                     FTGIELAGAMVDERPKYAALAGVKPEQIEIICVEAATRILPMFDDELAQYGVNLIKDL
                     GINLMLGSMIKEIKPGEVVYGTSKEDEELKSITAGTIIWTTGVSGSPVMGESGFDQRR
                     GRVMVNSDLRDPKYDNVYVIGDVSAFMDTESGRPFPTTAQIATRMGAHVAKNLLHQIK
                     GEATEDFSYSPQGTVASVGNTHGLGVVGKTKIKKYPASVMKKIIMNKSLVDMGGLKEL
                     LAKGRFDLYH"
     gene            complement(10974..11873)
                     /locus_tag="VBE22_000901"
     CDS             complement(10974..11873)
                     /locus_tag="VBE22_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEO7202372.1"
                     /translation="MTLPIFLELVEMKAKTASVLPFLIGLCFSAYYYNSINPVYVGLF
                     FVAMFLFNMFVDIWNNYNDYRNAVDLDYKNDTNIIGRENLSLRQIEVIMASLVITSSV
                     IGLVLVSQVGLPLLWMGLFCFGIGVLYSFGPRSLSSLPLGEVFSGLTMGFMISLICVY
                     LNTYQNFSWDILNLSKIFLISLPNTLWIANLMLANNLCDKEEDEKNHRYTLVHYTGIR
                     GGLLLFAISNSIALLAIVFEFLFGLAPVTVLLSLLLIPFIYKQTKLLWQKQVKRETFV
                     CAVRILALGSATQVLTYLIGIIF"
     gene            complement(12281..13204)
                     /locus_tag="VBE22_000902"
     CDS             complement(12281..13204)
                     /locus_tag="VBE22_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202373.1"
                     /translation="MKKLITEKKVNNVSTVNYLKLGLVSAMFAGGAFVALGSTQGVSA
                     STFTAPQATHPKAERQLTDSEIYERAQKQVLPKYIQGSLSGILNQHSTLYKQQNAAVT
                     PQVSSPKAERQLTDSELYESAQKQVLPKYIQGSLSGILNQHSTLYKQQNAAVTPQVSS
                     PKAERQLTDSELYESAQKQVLPKYIQGSLSGILNQHSTLYKQQNAAVTPQVSSPKAER
                     QLTDSEIYERAQKQVLPKYIQGSLSGILNQHSTLYKQQNAAVTPQVSSPKAERQLTDS
                     EIYERAQKQVLPNYIQGSLSGILNQHSTLNA"
     gene            complement(13809..13964)
                     /locus_tag="VBE22_000903"
     CDS             complement(13809..13964)
                     /locus_tag="VBE22_000903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202374.1"
                     /translation="MLVLLSVYTKDNGIGYQEELYMHKRLKWLVAATCLGFILEGRHL
                     ISKKWVK"
     gene            14087..14386
                     /locus_tag="VBE22_000904"
     CDS             14087..14386
                     /locus_tag="VBE22_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEO7202375.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQKQADLAASQFTGKSAQELVTVTFT
                     GDKKLISIDYKEAVVDPEDIETLQDMTTQAINDALSQVDDATKKIMGAFAGKMPF"
     gene            14547..15737
                     /locus_tag="VBE22_000905"
     CDS             14547..15737
                     /locus_tag="VBE22_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7202376.1"
                     /translation="MVMDSLIIKQLIKSTFDIPLQVTYPNGNIETYNGNNPHVKLKLN
                     KNFSVSELSKDPSIVLGEAVMDGDIEIDGSIQELILSAYRCGDSFLRNSKFSKLIPKQ
                     FHDKKHSKSDIQKHYDIGNDFYKLWLDDTMTYSCAYFKHENDSLEQAQLNKVHHILNK
                     LNAQPGGKLLDIGCGWGTLIITAAKEYGLNATGITLSEEQASFITKRIKEEGLENKVT
                     VLIKDYRDIHETYDYITSVGMFEHVGKENLSQYFQTISKRLNINGLALIHGITGQVGD
                     NHGSGTNSWINKYIFPGGYIPRLTENLNHIASAGLQIADLEPLRRHYQKTLELWTKNF
                     HNALPEVQKTHDKRFINMWDLYLQSCAASFESGNIDIFQYLLSKGVSKDTMPMTRDYM
                     YSAN"
     gene            complement(15807..16532)
                     /locus_tag="VBE22_000906"
     CDS             complement(15807..16532)
                     /locus_tag="VBE22_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7202377.1"
                     /translation="MSEKYSTGDLAKEAGVTVRTVQYYDKRGILSPSELSEGGRRVYS
                     IADLEKLRQIIYLRDLDFSIDNIKNLFTEDNASQILELFLQVQIRELRLAIDSKKDKL
                     DKAVNLLKTVEKQDSKTLGYLSDIVLMEENKRKWGKLQKSIVLQVVMIIMVYITLIIA
                     AVSLNQRWLMWIAVTVFLIAMNGMVWYFKQQVVYLCPNCHQTFTPTYQAFNLAKHTPK
                     TRKVFCPHCHQKSHCIELAREER"
     gene            complement(16659..17246)
                     /locus_tag="VBE22_000907"
     CDS             complement(16659..17246)
                     /locus_tag="VBE22_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEO7202378.1"
                     /translation="MKQLQEYIAFDLEFNTVGEHSHIIQVSAVKYSNHQEIALFDTYV
                     HTKVPLQSFINGLTGITARDIIGAPKIETILTDLQSFVGDTPLIGYNGHKSDLPLLVE
                     NGLDLTSQYQVDLYDEAFVRRSTDLNGIVNLKLTTVADFLGIKGKAHNSLEDARMTAR
                     VYEKFLDLDENKIYLKQQKEVAVDSPFAALGNLFD"
     gene            complement(17290..17826)
                     /locus_tag="VBE22_000908"
     CDS             complement(17290..17826)
                     /locus_tag="VBE22_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEO7202379.1"
                     /translation="MTVEKTVSELAEILGVSRQAMNNRVKTLPEECVEKNSKGVTVVN
                     RDGLIKLEEIYKKTILEEEPIDEEASRRELLEILVDEKNTEITRLYEQLKAKDIQIAS
                     KDEQLHVKDIQIAEKDKQLDQQQQLTLTAMEDTQRLQLELNEAKAEVEEIQEAKEEKI
                     QELEAVKKSFFGRFFNKK"
     gene            17961..19133
                     /locus_tag="VBE22_000909"
     CDS             17961..19133
                     /locus_tag="VBE22_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7202380.1"
                     /translation="MKHYDTIIIGGGPAGMMAAISSNFYGNKTLLIEKNKRLGKKLAG
                     TGGGRCNVTNNGNLDELLAGIPGNGRFLYSVFSQFDNHDIINFFQDNGVTLKEEDHGR
                     MFPTTDKSRTIINALENKIKELGGQIMTDTEVVSVKKIGDSFYIKTKDTQFASDKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLEVTDMEAAESPLLTDFPHKKLQGISLDDVTLSF
                     EKHIITHDLLFTHFGLSGPAALRISSFVKGGETIYLDVLPNISVKELETHFQNEREKS
                     LKNALKILLPERLAEFYAEDLPEKVKQVSVKDLETLIQKLKKLPILVTGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVAGLHFAGEVLDINAHTGGFNITSALCTGWVAGSLHY"
     gene            complement(19177..19959)
                     /locus_tag="VBE22_000910"
     CDS             complement(19177..19959)
                     /locus_tag="VBE22_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEO7202381.1"
                     /translation="MCYNNSMNERYIRFNPNIAKQKPSNKDKCPFCDKSQLGKILDVK
                     DDMIWVENKYPTLEETYQTLVIESNDHNGDISVYSESKMRQLLDYLLSKWQLMEESGH
                     YRSVVLYRNFGPLSGGSLRHPHSQIVGLKNVDAYETLNLDWFDGIEVCSETSQQAEIS
                     IANRPMLDFTEFTIKVSKLENAILIADSLPILCHYILNEFLGGRCKSYNLFFYKHDGG
                     LICKIVPRFIVSPYQIGYAIHQVNLPDELEKIKEALRAKYYL"
     gene            20118..20387
                     /gene="rpsN"
                     /locus_tag="VBE22_000911"
     CDS             20118..20387
                     /gene="rpsN"
                     /locus_tag="VBE22_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEO7202382.1"
                     /translation="MAKKSKIAKFQKQQKLVEQYAELRRELKEKGDYEALRKLPKDSN
                     PNRLKNRDLIDGRPHAYMRKFGMSRINFRNLAYKGQIPGIKKASW"
BASE COUNT         6880 a         3928 c         3253 g         6390 t
ORIGIN      
        1 tcagctacat tccaactaaa cctaacgaag cttttgaaga gctcaaagga ctaggttttc
       61 cattaaacaa aaagaattct gataaagcta atttggaagc ttttctcaga cattcttttt
      121 taaatcaaac tgatactgat tacgctctta gtctccttat cgttgatgca aaaaccgatg
      181 ctctaacctt ttttaaatca aatagtgact taacactaga aaatttacaa tggatttatt
      241 tacagttatt aggctttgtc ccctttgtag actttaaaga ccctaaagct ttcttacaag
      301 atattaactt cccagtctca tatgacaata tttttcaaag tctacatcac ttactcgcct
      361 gtcgtggaaa atctggcaat acattaatcg accaattagt tgctgatggt ttacttcatg
      421 cagataatca ctaccatttt ttcaatggga agtctctggc cactttcaat actaaccaat
      481 tgattcgcga agttgtctat gttgaaatat ccttagatac tatgtctagt ggtgaacatg
      541 atttagtaaa agttaacatt atcagaccca ctaccgagca tactatcccc acgatgatga
      601 cagctagccc ctatcatcaa ggtatcaatg atcctgccgc agaccaaaaa acataccaaa
      661 tggagggtgc gctagcagtt aaacagccta aacacataca agttgacaca aaaccattta
      721 aagaagaagt aaaacatcct tcaaaattac ccatcagccc tgcaactgaa agcttcacac
      781 acattgacag ttatagtctc aatgactatt ttctttctcg tggttttgct aatatatacg
      841 tttcaggtgt gggtactgct ggctctacgg gtttcatgac cagtggggat taccaacaaa
      901 tacaaagctt taaagcagtc attgattggt taaatggtaa ggttactgca ttcacaagtc
      961 ataaacgaga taaacaagtc aaggctgatt ggtcaaacgg ccttgtagca accacaggta
     1021 aatcttatct cggtaccatg tcaactggtt tagcaacaac tggcgttgag gggctgaaag
     1081 tcattatcgc tgaagccgca atctccacat ggtatgatta ttatcgagaa aatgggcttg
     1141 tgtgtagtcc aggcggctac cccggtgaag atttagacgt tttaacagaa ttaacatact
     1201 cacgaaacct cttagctggt gattacatca aaaacaacga ttgctatcaa gcattgttaa
     1261 atgaacaatc aaaagcaatt gaccgtcaaa gtggggatta caaccaatac tggcatgacc
     1321 gtaattacct aactcacgtc aataatgtca aaagtcgagt agtttacact catggactac
     1381 aggattggaa tgttaagcca agacatgtct acaaagtttt caatgcattg cctcaaacca
     1441 tcaaaaaaca ccttttttta catcaaggtc aacatgtgta tatgcataat tggcagtcga
     1501 ttgattttcg tgaaagcatg aatgccttac taagccaaga actacttggc attgacaatc
     1561 atttccaatt agaagaggtc atttggcaag ataatactac tgagcaaact tggcaagttt
     1621 tagatgcttt cggaggaaac catcaagagc aaattggttt aggtgatagt aaaaaactta
     1681 ttgataacca ttatgacaaa gaagcctttg atacttattg taaagacttc aatgtgttca
     1741 aaaatgatct tttcaaggga aataataaaa ccaatcaaat cactattaat cttcctctaa
     1801 agaaaaatta tctcctgaat ggacagtgca aactccatct acgtgttaaa actagtgaca
     1861 aaaaggccat tttatcagcc caaatcttag actatggtcc taaaaaacga ttcaaagata
     1921 caccaaccat caaattctta aacagccttg ataatggtaa aaattttgcc agagaagctt
     1981 tacgtgaact cccgtttact aaagatcatt atcgtgtcat cagtaaaggt gtcttgaacc
     2041 ttcaaaatcg tacagactta cttacaattg aggctatcga gccagaacaa tggtttgata
     2101 tcgagtttag cctccaacca agtatatatc aattgagtaa aggtgataat ctaaggatta
     2161 tcctttatac aactgatttt gaacatacca ttcgagataa tgctagttac tctataacag
     2221 tagatttgag tcaatcttat ttaactatcc caactaatca aggaaattaa cttatgaaac
     2281 ttcttactaa agaacggttt gatgattctc aacacttttg gtaccagatc aatttattac
     2341 aagagagtaa cttcggagca gtttttgacc atgataataa aaacattcca caggttgttg
     2401 caactattgt tgatgattta caaggttccg gaagttcgaa tcatttctgg tattttggca
     2461 atactactga tacttccatc cttatgattg ctcatttaaa tcgaaaattc tatattcagg
     2521 ttaatttaaa ggactttgac tttgcactca atttaatagc tataaataat tggaagagtc
     2581 tcctccaaac tcaacttgaa gctctaaacg ataccctagc aatatttcaa taaataaggt
     2641 agaatggagt gacaaagcaa cgcgagggag actgattaat gtcatcttat tggaataact
     2701 atcctgaact taaaaaaaat attgatgaaa ccaatcaact aattcaagaa agaatacagg
     2761 tcagaaataa agatattgaa gcggcgctaa gccaactcac agctgcggga ggaaaacagc
     2821 tcagaccagc attcttttac cttttttctc aacttggtaa taaggagaat caagatactc
     2881 agcaactaaa gaaaatcgct gcttctttag aaatccttca cgttgctaca ttaatccatg
     2941 atgatgtcat tgatgactca ccactaagac gtggaaatat gaccattcaa agcaagtttg
     3001 gcaaagacat cgcagtttat actggggatt tacttttcac agtctttttc gatcttattt
     3061 tagaatctat ggctgataca ccatttatga ggattaatgc aaaatctatg cgtaaaattc
     3121 tcatgggaga attggaccag atgcaccttc gttacaatca acaacaaggt atccatcact
     3181 atttacgtgc gatttcaggt aagacagccg aactctttaa attagctagc aaagaaggag
     3241 cttactttgg tggtgcagag aaggaggttg ttcgtctagc aggccatatc ggctttaaca
     3301 ttggtatgac attccaaatt ttggatgata tcctggatta tactgcagat aaaaaaacat
     3361 ttaataagcc tgtcttagag gatttaacac aaggcgttta cagccttcct ctacttcttg
     3421 ccattgaaga aaatcctgat attttcaaac ctattttaga taaaaaaaca gatatggcta
     3481 ctgaagacat ggaaaaaatt gcttatctcg tcgtttccca tagaggtgtt gacaaagctc
     3541 gccatctagc tcgtaaattt actgagaaag ctattagtga cataaataag ctaccccaga
     3601 actctgcaaa aaaacagttg ctacaattaa ctaattacct tttaaaacgc aaaatttaaa
     3661 taataaaaaa acattccaca atgctagaaa agcagttagg gaatgttttt ttattatcat
     3721 ttatttatcg cacctatcaa tcatcataga tcaccatcat cagcggcttt cagctgacgg
     3781 taacgttgac tactttgaga caattcttga ggagaacctt ccaactctaa ttgcccattt
     3841 tctataaata agatacgatc agcatgttca atacctttta agtgatgtgt aatccaaact
     3901 aaggtcttac cttccaattc tttcataaat acccttagta aggcttgttc agtaatagga
     3961 tcaagtccaa cagttggctc atctaagata acaattggga catcttttag taagattcta
     4021 gccaaagcaa ttctatgcct ttcgccacct gaaaacctaa gtccagcttc atcaaccatt
     4081 gtatagagac catctgataa atcagtgacc atctctttca atccaactcg ttcaagaact
     4141 ttccatacat cttcttcact agcatcttgg tttccaatgc gaatgttatt tagcagggtt
     4201 gtattaaaaa ggtagggcgc ttgttgtatc actccaatat agttagaaat gcaatcacca
     4261 actattgaaa catcagcacc gcctagggta atcttccctt gacttgcttt caagtcgcca
     4321 cgaagtagac tagctaaggt actcttgcca gaaccactcc gccctaaaat agcaattttt
     4381 tctccttctt taatatccaa atctaaatga tgcaaaaccc atttctcttg tggcttatac
     4441 tggaaactta aattcttgac ggaaaaatca tatggcttat taggcaattg attttgagtt
     4501 gtttcaaaat acgtttctga taattcatta agacgatgaa ttgaatcaga atatttattt
     4561 gtttcttgag cagcagcaga caaaccagca aatgcctctg acaagggaaa tactgttaga
     4621 acaaatgctg caatccagtt agcttcgcca cctctgtgac caataaactg attactagcc
     4681 caaataataa ccaaaatagc tagaaagcca aaaactaatt ccacaataag agctctacga
     4741 ttattaaagg aacgaatttt tttctgaaca gccattagtt cactttccga acgttcatgc
     4801 aaggcgacgt actcttgacc acgttgacta aaaatccagt ctgaaatacc taagacatta
     4861 tccgtcaaat cagtataaag atgatttttt aattcttttt cacgagtctg acgagcacca
     4921 ttagctaaaa tcgaccaaag tggaaaaaga aaaatcataa tagctaaata aagcaacatc
     4981 attaaagcaa accatagaga aaagaagcct gttgcaataa taataaagct atataataac
     5041 cacgctataa tcgttggaaa tattgtccta agatacaaat tttgcaaata attaatatcc
     5101 tccgctaaaa gccccataac atctcctaaa cgaaaatcac gcttcataaa gatagcattg
     5161 ctttctaagg aatggtaaag cttcaagcgc agttgtgaag tcattcgtaa tacccaattg
     5221 tgactcgtta ggcgttctat ataacgaaaa acaggacgtc caataccaaa agcacgtgtt
     5281 agtacaattg gaacatatac tagaagaata ttactcggta gtgaagctga cttactaatc
     5341 agatagcccg aattaaacat aagagcactg gcagaaaaga aagtcataaa accaaggaaa
     5401 agagcaatta ctaaggaaac tttatattgt ttgaaaaatg gttttaccca ttgatctgtc
     5461 tttaattctt taaacaaagg gattttaaac attatttcct cccatagcat gcttcagttg
     5521 atagagatat ccttttttag ctaataattc ctgataagag cctacttcta caactctccc
     5581 cttctccata acgactataa cgtccatttg gttcaaccag tgaagacgat gagttgcaaa
     5641 gataactaaa cgatctgaca tcagtggcaa cattttctct tttaattcta actctgtttc
     5701 aatatctaaa tgagcagttg gttcatcaaa aaccataata cgacgatttt gatcaagaaa
     5761 agcacgagcc agagctatac gctgggcttg tcctccacta agtggacgcg ctccattacc
     5821 aatacgcgtt tctaaaccat cgggtaactc gcttagtaag ctatctaaac ctaccatatg
     5881 aatcgctctt atcacctctt cgtcactagc attgggagta tagaaggtaa tattatcacg
     5941 taatgacatt tcaaaaacat aaggagtttg aggaatatag atcatctgtt tatgccaatc
     6001 ttcttgatca aggttaacca cttctctacc atccactttt aaagaacctt tatcaggccc
     6061 taaaaaacca ctcagaaggt taattaagct agacttacct gaaccactaa ctcctactaa
     6121 agcaaccttt tgatgtcctc taaatcgtaa ggttacatcc tcaacaacac gtttttggtc
     6181 gtacgcaata gcaatgtttt ctaaatccaa atgactctct ttagaccaat catctatgac
     6241 taattgctca cctttaatac cagttttgtt tagaaccttt tgaatcgctt gaaatgcatt
     6301 tttcccgtct aacgttgcat gataatcact agaaaaatct cttacgggta aaaagtattc
     6361 aggggataaa atcaaaattg ttaaagccgg taaaaggtaa atctgctcat ttaataatcg
     6421 taaacccaga aaaacagcaa cgatagcaat ggataaagtt gtaaaaaagt ctaaagcaaa
     6481 cgttgataaa ataccaattt tcaaagtact cattgttgct ttacgaaaag attcacttgt
     6541 ttgataaatg cttttaccat aacgtttact aagaccaaaa tatttgagag tatcaatgcc
     6601 tcgtaatgaa tctaaaaaat gattagaaag cacttgatag gattcatatt gtttatctgc
     6661 tttagcttga gccgcatagc ctaaaataat cataaagatg ataattaaag gaaaaacgat
     6721 aagtaaaatt gcacctgatt caatatctaa atagaaaata taagctagaa taatccatgg
     6781 gataatagac atgttaatca tcttattcaa gactaaacgt aaataatttt caactaaaga
     6841 aacaccatct agtgccattg taacaacatt accagttcct tgttcttgaa cgactttagg
     6901 acctagatca aataacttat ctaataattg acgtctcaaa agactagatt gtgctgttga
     6961 aaagtcatca agctttctat ttttgatgta atcaataaca tgacggccta aatatgagac
     7021 cataaataac aaaatataaa ccgtttgaga agaaagtttt tgcccttccc acaaaccagt
     7081 tatagaaaga ctcaaataat aagcttgtcc gataataaag attgcttgaa gaacatcaag
     7141 tcccgcaagc aatcctaaca acttatgaat acctgacaag cgcataaccg ctttatctag
     7201 cattattttt ctcctgtcac aatcactggc aaggtaatac gttttctaaa gatataatat
     7261 gcccatgctg tataagctag aacaaaggga actagagtaa ttgcgacaat cgacattact
     7321 tttaaagtat atggcgttga ggatgcattt tgaatcagca aatcatattt agggctaatt
     7381 gatgaaatca taactcgtgg gaatagacct tggaacaaca gaacaaccac tgaaacaaga
     7441 ctgagaccac tcgctaaaaa tgctgtcatc tctgctcctt taaaagttga agcatgcgct
     7501 aatacagcta atacaacaat aaccaaaacc ataattgtcg taactatcgg atgattgcta
     7561 aagaaatctg tcttgaaata caagagaact gcaaacaagg ctaaaccaag atagagtacc
     7621 cagtaaagca actgagcata attacgggca cgctctctaa tcggcccttc tgtcttcaat
     7681 gcaatgtagt ttaaaccatg aagataggct agcaatacca ttgcaactcc acccaccaac
     7741 gagaatatat taaagtaatc actgaattga gctgataagt taccactggc atcaagtggc
     7801 ataccttgaa tcaaacttat aaacataatc ccaaagaaga atggaacaat agcggaacca
     7861 attgttaagg tccagttcca aaactgcttt ttctctgcag gaactttatg tctaaattcg
     7921 aaggaaacac cacgaataat taatccaaag agaatcgtaa ggagaatcaa atagtaacca
     7981 ctaaatagtg aagcatacca gtatggaaaa gaagcaaaca ttgctccacc accggtaaga
     8041 agccaaactt cattaccatc ccaaactgga ccaatggttt ctactacttg atctttttca
     8101 tgctcattat gtgttaaagt ttgaacagcc atcccaaccc caaagtcgaa tccttcaagg
     8161 aagaagaatc ctgaaaatag gaggccgatt aggaaaaacc aaaagaattg taaagcactc
     8221 atttagtatg ctcctttctc aaagggatca attgaagtat atgcaccatg atgtccatgt
     8281 tcatcttcat actctggccc tttcttcaat tctcttattg ttaggcttat catcatcacc
     8341 gctaaaccac caaacaataa gaagtacaca gtattggaaa caaataatga tgcaactgaa
     8401 acgtttggag agacagagtc cttaatcttg aaaagaccat agacagtcca agggtatcgt
     8461 ccttgttctg tcacaatcca accaaatgta ttagctaaga atggtgcaaa ggttgttaaa
     8521 gcaacaatcc atagcatcca tttgtgttta taaagaattg gttttttctt acgagttaaa
     8581 aagagtccta aaacagaaac acccataata gctgttccca tcgccgccat tgtacggaaa
     8641 ccatagaaaa gtaggttaac cattggatag tagttatctt taccatactt agcaaccaat
     8701 tctttattag ctgtatccat acctttcaca gaaccagatg gtttcccgta agataaaata
     8761 ctcaacatat aaggaatctt gatcccaaaa acttgtttgt gttctgcttc atttgcccaa
     8821 gcaactacac tccaagctgc aggatcaccc gaatcttcat aatcaccttc catagcagca
     8881 aatttcattg gttgatcgtg aatcaaggct ttcatttgta aatccccaac tcccataact
     8941 gaaattgaac caagaagtgc tactactaaa ccaatacgaa ctgatttctt ataaagttca
     9001 accgttgtat ctttcagctg ttctgatttt aagaggcgga aggctgacat accagcaacc
     9061 actgtaccac ccatcgtaat ggcaccaaaa ataacatggg taaattcata gaagaactgt
     9121 ggatttttta caagtgcaag aaaatcagtc atttgcgctc taccgtttac aacttcataa
     9181 ccaacaggat gttgcatgaa gctattagca gtaagaatcc acatcgctga cattaatgag
     9241 ccaaaaacca ctagccaaat gaatgtcaca tgtaattttt tactaatttt tttgttatcc
     9301 catgtaaaca tccataaacc aaggaaagta gattccataa agaaagctaa taaagcttca
     9361 atagccaatg gagctccaaa aatatcacct acgaaacgtg aatagtctga ccagttcatt
     9421 ccaaattgga actcttgaat gatacctgtc acaacaccaa ctgcaaaact aaggagcatg
     9481 atattacccc aaaattttgt caatttttta tactcttcat tttttgtgat aacatacatt
     9541 gtctccataa tagcaaccac taggcatgta ccaattgtaa atggtacaaa gaaaaagtgg
     9601 aaaactgtag tcatcgcaaa ttgaaaacga gctaaagttt caattgtcat acaaaacctc
     9661 ttattttgtt ttaatcattt cttttataga aatatctgca agagctagtt tcctagcttc
     9721 gtaacaattg gtaaataact tactttaata tcttaatgat ataaatcaaa acgtccctta
     9781 gctagaagtt ctttaagtcc acccatatca acaagtgatt tgttcatgat aatcttcttc
     9841 ataaccgacg cagggtattt tttaatttta gtttttccaa caacaccaag tccatgcgta
     9901 tttcctactg aagcaactgt gccttgcgga ctatacgaaa agtcttctgt agcctcacct
     9961 ttgatttgat gtaaaaggtt tttagcaaca tgagctccca tacgagttgc aatttgagct
    10021 gttgttggga atgggcgacc agactcagtg tccataaatg cagacacatc accgataaca
    10081 taaacattat catattttgg atcacgcaaa tcactgttga ccattacacg tccacgacgt
    10141 tgatcaaatc ctgattctcc cataaccgga cttccactaa cacctgttgt ccaaatgata
    10201 gtacctgcgg taatagattt gagttcttca tcctctttac tagtaccata aacaacttca
    10261 cccggtttga tctccttaat catacttcca agcatcaaat taatacctaa atccttaatt
    10321 aagttgacac catattgcgc taattcatca tcaaacattg gtaggatgcg agttgctgcc
    10381 tctacacaaa taatttcaat ttgttcaggt tttacacccg ctaaagcagc atattttggt
    10441 cgctcatcaa ccatagctcc ggctaactca atacctgtaa aaccagcacc acatactaag
    10501 agtttgagaa gattttcatc ttttgtctca cgatacaact tcattgtatt aacaatgtga
    10561 ttatggatgt tttctgcttg cgaaatattg gtcatctgca aagcattctc catagcaccc
    10621 ttgatgccaa aagtttcaga tacaaagcct aaggcaacta cgacataatc ataatggagt
    10681 tcaccgttat ttttaactgt tactgtttta ttttcagcat cgactttaag cacctcatct
    10741 tgcatgaagt taaccttatt agtgttgata acatccttaa ttgggaatgt aattttttct
    10801 tttggctgag agcctgcagc tacttcatgt aattctgtag cttcgtagtg ataatcattc
    10861 atgtcaacaa gggtaatatg aaaatcacct gattgttttt gtaaattacg aactgctttc
    10921 aaaccggcat aaccagcacc taaaactagg atttctttca tgataaaact cctctaaaaa
    10981 atgattccaa ttagatacgt taagacttgg gttgcagatc ccagtgctag aatacgtacc
    11041 gcacaaacaa atgtttctct cttaacctgt ttttgccaca atagttttgt ctgcttataa
    11101 ataaatggta ttaataacaa actcaagagt actgtaacag gcgcaagtcc aaaaagaaat
    11161 tcaaaaacaa ttgccaataa agctatacta ttagaaatag caaacagcaa gagtccacct
    11221 cttatccctg tataatgtac aagagtatag cgatgatttt tttcatcttc ttctttgtcg
    11281 cacagattat tcgccaacat taggttggca atccataagg tgttcggcaa ggaaatcaag
    11341 aaaattttgc ttaagttgag gatatcccac gaaaagtttt gataagtgtt tagatataca
    11401 caaattaaac taatcataaa tcccattgtc aagccagaaa atacttctcc taagggcaga
    11461 ctagataaag atcttggacc aaaggaatat aaaacaccaa ttccaaaaca aaaaagtccc
    11521 atccataaaa gaggcagacc gacttgggaa actaaaacta aaccaatcac actagaagta
    11581 atgactaaac ttgccataat aacttctatt tggcgtaggg ataggttctc acgaccaata
    11641 atgttagtgt cattcttata atccaaatcc acagcattcc gataatcatt gtaattattc
    11701 caaatgtcaa cgaacatatt gaataaaaac atagcaacga agaataaacc gacatataca
    11761 gggtttattg aattgtagta ataagcacta aaacacaaac caatcaaaaa tggtaaaaca
    11821 ctagctgttt tggctttcat ttctaccaac tctagaaaga ttggtaatgt cattattaaa
    11881 gtcctctcta tacataatct tcaacaatac tattataatg caatttctac catttttaaa
    11941 acaatagggc ttgatttttg attaaaaata caaaaaattt ttcttaaaat gagttaatca
    12001 cacttagaac actaaatact attttgagga gtcctattca tcacactatg ccatgtaata
    12061 ttcggaaaaa tagtattgca accttcaatt actccttatg ttggcaccag cctttgaaag
    12121 tcctctgatt gcactgctta gttagtattt tcatcagctg tatggtctct aagagccatc
    12181 agcatactaa aaaactagtg ttacactttg aaaagtgtaa cactagctcc aatttgtttt
    12241 aaatagctta actatttaat cattaatcta ctttatatat ttaagcattc aaagttgaat
    12301 gttggttaag gatacctgag agagaacctt gaatataatt tggcaatact tgtttttgag
    12361 cacgttcata gatttctgaa tctgttaact gacgttctgc tttggggctt gatacttgcg
    12421 gtgtcactgc tgcgttttgt tgtttgtaaa gtgttgaatg ttggttaagg atacctgaga
    12481 gagaaccttg aatatatttt ggcaatactt gtttttgggc acgttcatag atttctgaat
    12541 ctgttaactg acgttctgct ttggggcttg atacttgcgg tgtcactgct gcgttttgtt
    12601 gtttgtaaag tgttgaatgc tggttaagga tacctgagag agaaccttgg atatattttg
    12661 gtaatacttg tttttgggca ctttcataaa gttctgaatc tgttaactga cgttctgctt
    12721 tggggcttga tacttgcggt gtcactgctg cgttttgttg tttgtaaagt gttgaatgtt
    12781 ggttaaggat acctgaaaga gaaccttgga tatattttgg caatacttgt ttttgggcac
    12841 tttcataaag ttctgaatct gttaactgac gttctgcttt ggggcttgat acttgcggtg
    12901 tcactgctgc gttttgttgt ttgtaaagtg ttgaatgttg gttaaggata cctgagagag
    12961 aaccttggat atattttggt aacacttgtt tttgggcacg ttcatagatt tctgaatctg
    13021 ttaactgacg ttctgctttt ggatgtgttg cttgcggtgc tgtgaaagtt gaagctgaaa
    13081 caccttgtgt agaaccaaga gctacgaatg ctcctccagc aaacattgct gagacaagac
    13141 cgagtttcaa atagttaaca gttgagacgt tgttgacttt tttttctgta ataagttttt
    13201 tcataagaat tacctctttc ttttgggtta tttattttat gaactaagta tagcaacaag
    13261 aaataaagta aggttaaact aaatataaaa taattcttaa gtcattctta ggaaaccata
    13321 aaatcataca ttaacaaaaa atttggtatc tagatcaaaa gtaaaggtag aataaaggct
    13381 tttacttctg agctagtaat gaataaatta tatttgcatc tagtttggaa ttagccctag
    13441 tgtattataa tgattcctac aaatatgtgc caaacacctt cttcatatat acaagattga
    13501 tttaacattc ctataccctt aatggtgggc acattctctt catgttccat atagaaatca
    13561 acagataatt ccgtattgtc cctattatta aattttccag ttaatgataa attatatcct
    13621 ccaccttgag gcgccctatt accaatctgt tcaaccttta cacttgacga atcgaattga
    13681 acagattcta tcttatcatt ctgtgatttt agaacatcaa caatttcata ttcatgttct
    13741 ttgaggtaag ctagttgttc ttctttaacg tgcttttatc tttcgtactt actgatactt
    13801 gagagcattt atttgaccca cttcttacta atcagatgac gtccctctaa gatgaatcct
    13861 aaacacgtag ctgccactaa ccattttaac cttttatgca tatataattc ttcttgatat
    13921 cctataccat tatctttagt ataaacactt aataaaacaa gcatttaaca actacggtta
    13981 ataaaattca ataaatctaa tccttttact tactttagtt tatcactttt ttctctcatc
    14041 ctatgctata atagtaaggt atcaaaattt aggagaatat agtcttatga tgaatatgca
    14101 aaatatgatg cgtcaagcac aaaagcttca aaaacaaatg gaacaaaagc aagctgattt
    14161 agcagctagc cagtttactg gaaaatctgc tcaagagctc gttactgtga catttacagg
    14221 cgataaaaag ctaatttcaa ttgattacaa agaagctgtg gtagaccctg aagatattga
    14281 aacactacaa gacatgacaa ctcaagctat caatgacgct ttaagccaag ttgacgatgc
    14341 tacaaaaaaa attatgggag catttgcggg taaaatgcca ttctaaaaaa taaatcacat
    14401 ttactgaatc acctgtaaat gtgttttttt atttcaaatc actttcattt ttcatgaaaa
    14461 taagttttat tttcaaaaaa atatttgcaa aaaatcagca gagaggtata ataacattga
    14521 aaatattaga gagaaggtga ctaaccatgg ttatggatag tctaatcata aaacagttga
    14581 ttaagagtac atttgatata cccttacaag tcacttatcc aaatgggaat attgaaactt
    14641 ataatggtaa caatccccac gtcaaactta aactaaacaa aaatttctcc gtctcggagc
    14701 tatcaaaaga cccttctatt gtattgggtg aagctgttat ggatggagat attgaaattg
    14761 atggttctat acaagaatta attttatcag cttaccgctg tggagacagc tttctacgta
    14821 atagtaaatt tagcaaattg attcctaaac aatttcatga taaaaaacac agtaaatccg
    14881 acattcaaaa gcattatgac atcggaaatg atttctataa actttggctt gatgatacca
    14941 tgacttattc atgcgcctat tttaaacatg aaaatgacag tctagagcaa gctcaactaa
    15001 acaaagtaca ccatattctt aataaattaa atgctcagcc gggagggaaa ttactagata
    15061 tcggttgtgg ctgggggaca cttatcatta ctgctgctaa agaatatggc ttaaatgcaa
    15121 caggtattac cttaagtgaa gagcaggctt ccttcattac taagcgtatc aaggaagaag
    15181 gattggaaaa taaagtaaca gtcctcatta aagattatcg tgacattcat gaaacatacg
    15241 attatatcac tagtgttggt atgtttgaac atgtaggaaa agaaaattta tctcaatact
    15301 tccaaactat ttctaaaaga ctcaacatca atggtctagc tcttattcat ggtattactg
    15361 gacaagtggg tgataatcat ggtagtggaa ctaattcttg gattaataag tatatcttcc
    15421 caggaggata tatccctcgt ttaactgaaa atttaaatca tatagctagt gctggacttc
    15481 aaattgctga cttagaaccg cttcgtcgtc actatcaaaa gactctcgaa ttatggacaa
    15541 aaaatttcca caatgccctt cctgaagttc agaaaactca tgataagcgt tttatcaata
    15601 tgtgggacct ctatcttcaa tcttgtgcgg cctcttttga atctggcaat attgatattt
    15661 ttcaatactt gctttcaaaa ggtgtttcaa aagacactat gcctatgaca cgcgattata
    15721 tgtattctgc taattgatat agttaatatt atagtattcc aacttattct aaataaaaag
    15781 aactctcttg aattcaaggg agttctttaa cgttcttctc tggctaactc tatacaatgt
    15841 gacttttggt gacaatgtgg acagaatact tttcgcgtct taggggtatg tttcgctaga
    15901 ttaaatgctt gatatgtcgg tgtaaaggtt tgatgacaat ttggacaaag ataaacaacc
    15961 tgttgtttaa aataccaaac cataccattc atagctatta agaatactgt tactgctatc
    16021 cacataagcc atctttgatt tagagaaact gctgctatta ttaacgtaat atagaccata
    16081 ataatcataa cgacttgtag cactatgctt ttttgtagtt taccccattt tcttttgttt
    16141 tcttccataa gcacaatgtc agaaagatag cctaatgttt tactgtcttg tttttctacg
    16201 gtttttagta ggttgacagc tttgtcaagt ttgtcttttt tactatcaat agctagcctt
    16261 aattctctta tctgcacttg taaaaaaagc tctaaaatct ggctagcatt gtcttcagta
    16321 aatagatttt tgatattatc aattgaaaaa tctaaatctc gtaaataaat aatttgtcta
    16381 agcttttcta aatcagcaat actataaacc ctcctacctc cttcagatag ttctgatggg
    16441 gataaaattc ctcttttgtc atagtactga acagtacgaa cagtaactcc tgcttcttta
    16501 gctaagtcac cagttgaata tttttctgac atgtttcaca cctcctttaa tatagtaaac
    16561 acttctttat cagaaacacc tttactatat caaatgacct aaggtaataa gcaagtaata
    16621 acgctaagta tttttaactt taaataaaac tacaacaatt aatcaaacaa atttcctaaa
    16681 gccgcaaatg ggctatcaac tgctacctct ttttgctgtt ttaaatagat tttattttca
    16741 tccaagtcca gaaatttttc ataaacacgc gcagtcatgc gggcatcctc taagctatta
    16801 tgtgccttac cctttattcc taaaaagtca gcaacagtag tcaatttaag gttgacgata
    16861 ccatttaaat ctgtactacg tctcacaaaa gcttcatcat ataagtccac ctgatattga
    16921 ctagttagat ccaaaccatt ttcaactaaa agaggtaaat ccgatttatg cccattataa
    16981 ccaatcaacg gtgtgtctcc aacaaaactc tgcaagtctg tcaaaattgt ctcaatttta
    17041 ggagccccta taatatctct agctgtaata cctgttaaac cattaataaa gctttgtaat
    17101 ggaacttttg tgtgaacata agtatcaaat aaagcaatct cttgatgatt agaatattta
    17161 acggccgaca cttgaataat atggctatgc tcaccaaccg tgttaaactc taaatcaaaa
    17221 gctatatatt cttgtaattg tttcattatt tctcctaaaa agcgcaagga aactccttac
    17281 gcttttattc tattttttat tgaagaaacg tccaaagaaa ctttttttaa cagcttctaa
    17341 ttcttgaatt ttttcttctt tggcttcttg aatctcttca acctctgctt tagcttcatt
    17401 taactcaagt tgcagacgtt gcgtatcttc catagcagtt aaagtcaatt gctgttgttg
    17461 atctaattgc ttatctttct ctgctatttg aatatctttt acatgtaact gctcgtcttt
    17521 tgaagcaatc tgaatatctt ttgcttttaa ctgttcatat aaacgtgtga tttcagtatt
    17581 cttctcatca acgagaattt ctaaaagttc tcgtcttgat gcttcttcgt caattggttc
    17641 ctcttccaaa atagtttttt tatagatttc ttccaattta atcaagccat cacgattaac
    17701 aactgtaacg cctttactat tcttttccac acattcttca gggagtgttt taactctgtt
    17761 attcatcgct tgacgactaa cccctaaaat ttcagctaat tcactaaccg tcttttcaac
    17821 tgtcataact tcctcttttg tactgatttt cttataaaag aatatcacaa tcctaatcta
    17881 gtgtcaattt actgtcaact taaagtgtca aacaagacat tttgacgtct atttctctat
    17941 ttatatgcta gaataagatt atgaagcatt atgacacaat aattataggt ggtggacctg
    18001 ctggaatgat ggcagctatt tcctcaaatt tttacggcaa caaaacactt cttattgaga
    18061 aaaataaacg tttaggcaaa aaactagctg gaacaggtgg tggccgctgt aatgttacca
    18121 ataatggaaa ccttgatgag ctcttagctg gtatacctgg aaatggccgt ttcctatata
    18181 gcgtcttctc acaatttgat aaccatgata ttattaactt ctttcaagac aacggcgtca
    18241 cacttaaaga agaagatcat gggcgtatgt tccctactac tgataaatct cgtactatca
    18301 ttaacgccct tgaaaataaa ataaaagaac ttggtggtca aattatgact gacacagaag
    18361 ttgtctctgt caaaaagata ggagattcat tctatatcaa aactaaagat acacagttcg
    18421 catccgataa acttatcgta acaactggtg gtaaatccta tccgtctaca ggttctactg
    18481 gtttcggtca tgacattgct cgtcacttta aattagaagt cacagatatg gaagccgccg
    18541 aaagccctct tttaactgat tttccccata aaaaactaca aggtatctcc ttagatgatg
    18601 tcactttgtc atttgaaaag catattatta cacacgatct tctctttact cattttggat
    18661 tatcaggacc agcagccttg cgaatttcca gctttgtcaa aggcggagaa actatatacc
    18721 tagatgtttt gcccaatatc tctgttaaag aattggaaac acacttccaa aatgaaagag
    18781 aaaaatcttt gaaaaacgct cttaaaatac ttcttcctga gcgtttagct gaattttacg
    18841 ctgaagactt accagaaaaa gtaaaacaag tatctgtaaa agacttagaa acgttaattc
    18901 aaaaacttaa aaaacttcct attttagtaa ctggtaagat gtctctagca aaatcctttg
    18961 taacaaaagg gggagttgat cttaaagaaa taaaccccaa aacacttgaa agtaaaaagg
    19021 tagctggttt acattttgct ggtgaagtcc tagacatcaa tgctcatacg ggagggttta
    19081 acattacttc tgccttatgt acaggttggg tagctggtag cttacactat taatacatga
    19141 aagctgaaca attccgtcag cttttttata tagaatttaa agataatatt tggctcttaa
    19201 ggcttctttg attttttcca attcatctgg taaatttacc tgatgaatag catacccaat
    19261 ctgataaggg gatactataa aacgaggaac aattttacaa ataagacccc catcatgctt
    19321 atagaaaaag agattataag atttacagcg ccctcccaag aattcattta aaatataatg
    19381 acacaaaatt ggtaaactat ctgcaatcaa aatagcgttt tccaatttgg atactttaat
    19441 tgtgaactct gtgaaatcaa gcataggacg attggcaata ctaatctctg cttgttgact
    19501 cgtttccgaa caaacctcga taccatcaaa ccaatctaag ttcaacgttt cataagcatc
    19561 tacgtttttt aagccaacaa tttggctatg aggatgtcgt aaacttccgc cagataaggg
    19621 cccaaaattt cgataaagaa cgaccgaacg gtaatgacca ctttcttcca ttaattgcca
    19681 ttttgacaaa agataatcta acaattggcg catctttgac tcactataaa ctgaaatatc
    19741 cccattgtga tcatttgatt caatcaccaa tgtttgatag gtttcttcta aagttgggta
    19801 tttattttct acccagatca tatcgtcttt cacgtctagg atttttccta attggctttt
    19861 atcacaaaat ggacatttgt ctttattact gggtttttgt ttcgcgatat tagggttaaa
    19921 tcggatatat ctttcattca tactgttatt gtaacacatt catcaccatt acaatataat
    19981 acttatccag ttaacatttt ccttgcattc ttagctgaaa agagtataat agaatcatgt
    20041 cttgtttact ggttaattac cagttaacta ggcaccctgt tttgggatag aaaaatcttt
    20101 ttgggaaagg agaaatcatg gctaagaaat caaaaattgc taaatttcaa aaacagcaaa
    20161 aactcgtaga acaatatgct gaactacgtc gtgagttaaa agaaaagggt gattacgaag
    20221 cacttcgtaa acttcctaaa gattctaacc ctaaccgact taaaaatcgt gatttaatcg
    20281 atggccgtcc tcacgcttac atgcgtaaat tcggtatgag tcgtattaac ttccgtaacc
    20341 tagcttacaa aggccaaatt cctggcatta aaaaagcaag ctggtaattc gtgaagacaa
    20401 atagttactt tttatgacta ttctattgag tgtggacgaa tagttaatac a
//
LOCUS       DATRTN010000023        20336 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.023, whole genome shotgun sequence.
ACCESSION   DATRTN010000023 DATRTN010000000
VERSION     DATRTN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20336
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.023"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..766
                     /locus_tag="VBE22_000912"
     CDS             <1..766
                     /locus_tag="VBE22_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7202383.1"
                     /translation="EAIKEIINSAKKRTYIFWLVIQTLFLVLISPILIKLGLSVFMIT
                     LLIFGLGIIKWLVITYKVKVFYNNQNLNWDAAINHEQERKQSILKFFSLFTNVKGIST
                     SVKRRSFLDVILKLISKTPSRLWTNLFVRAFLRSSDYLGLTIRLVTLNILSVIFVNET
                     YLALALAFVFNYLLLFQLLALGHHFDYQYMNQLYPVRLNAKASQLKGFLRVLSYAVTV
                     IDIILIRELEPVILLIVLMLIVTEYYIPYKIKKMID"
     gene            824..1624
                     /locus_tag="VBE22_000913"
     CDS             824..1624
                     /locus_tag="VBE22_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEO7202384.1"
                     /translation="MTISNQELTLTPLRGKSGKAYIGTYPNGERVFVKYNTTPILPAL
                     AKEQIAPQLLWARRTSNGDMMSAQEWLDGRTLTKEDMGSKQIIHILLRLHKSRPLVNQ
                     LLQLGYKIENPYDLLMDWEKQTPIQIRENTYLQSIVTELKRSLPEFRTEVATIVHGDI
                     KHSNWVITTSGLIYLVDWDSVRLTDRMYDVAYILSHYIPQKHWKDWLSYYGYKDNEKV
                     WSKIIWYGQFSYLSQIIKCFDKRDMEHVNQEIYELRKFRELIKKHNAS"
     gene            1614..2249
                     /gene="trmB"
                     /locus_tag="VBE22_000914"
     CDS             1614..2249
                     /gene="trmB"
                     /locus_tag="VBE22_000914"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEO7202385.1"
                     /translation="MRVRKRKGAEEHLENNPHYVISNPEEAKGRWHEIFGNNNPIHIE
                     VGSGKGAFITGMAEQNPDINYIGIDIQLSVLSYALDKVLDSGAKNIKLLLVDGSSLSN
                     YFDTGEVDLMYLNFSDPWPKKKHEKRRLTYKTFLDTYKDILPEQGEIHFKTDNRGLFE
                     YSLASFSQYGMTLKQVWLDLHASDYQQNIMTEYERKFSNKGQVIYRVEARF"
     gene            2293..2379
                     /locus_tag="VBE22_000915"
     tRNA            2293..2379
                     /locus_tag="VBE22_000915"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2327..2329,aa:Ser,seq:gga)
     gene            2738..3214
                     /gene="rimP"
                     /locus_tag="VBE22_000916"
     CDS             2738..3214
                     /gene="rimP"
                     /locus_tag="VBE22_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEO7202386.1"
                     /translation="MANQTIVDIVTQVVTPAIQAPFELVDVEYEKMGGDYVLSILIDK
                     PGGITVEDTAQLTDVVSPLLDTIQPDPFPEQYMLEVSSPGLERPLKTAEALSNAVGSY
                     INVSLYKSIDKVKIFEGDLLSFDGETLTIDYMDKTRHKTVDIPYQTVAKARLAVKL"
     gene            3241..4401
                     /gene="nusA"
                     /locus_tag="VBE22_000917"
     CDS             3241..4401
                     /gene="nusA"
                     /locus_tag="VBE22_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEO7202387.1"
                     /translation="MDTMSKEMLEAFRILEEEKHINKEDIIDAVTESLKSAYKRRYGQ
                     SESCVIEFNEKKADFTVYTVREVVDEVFDSRLEISLKDALAISSAYELGDKIRFEESV
                     TEFGRVAAQSAKQTIMEKMRRQMREVTFNEYKQHEGEIMTGTVERFDQRFIYVNLGSL
                     EAQLSHQDQIPGESFKSHDMIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEREIPEV
                     FDGTVEIMSVSREAGDRTKVAVRSHNSNVDAIGTIVGRGGSNIKKVISNFHPKRVDAK
                     TGLEIPVEENIDVIQWVEDPAEFIYNAIAPAEVDMVLFDDEDTKRATVVVPDSKLSLA
                     IGRRGQNVRLAAHLTGYRIDIKSASEYEKMEAQELQTEEVAQESEVISDKAE"
     gene            4423..4719
                     /locus_tag="VBE22_000918"
     CDS             4423..4719
                     /locus_tag="VBE22_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEO7202388.1"
                     /translation="MAKTKKIPLRKSVVSGEVIDKRDLLRIVKNKEGQVFIDPTGKQN
                     GRGAYIKLDNDEAILAKKKRVFDRSFSMEVSDEFYDELLAYVDHKVKRRELGLE"
     gene            4712..5014
                     /locus_tag="VBE22_000919"
     CDS             4712..5014
                     /locus_tag="VBE22_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEO7202389.1"
                     /translation="MNNSEKVLNLIGLAQRAGRLITGEELVIKAIQNQQVSLIFLAND
                     AGPNLTKKVTDKSNYYKTEVSTVFSTLELSDALGKPRKVVAVADAGFSKKMRTLMA"
     gene            5034..7817
                     /gene="infB"
                     /locus_tag="VBE22_000920"
     CDS             5034..7817
                     /gene="infB"
                     /locus_tag="VBE22_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEO7202390.1"
                     /translation="MSKKRLHEIAKEIGKTSKEVVEQAQSLGLPVKSHASSVEENDAT
                     RIVESFSSSKTKAPTNSVQTNQGVKTESKTVETKQGLSDDKPSTQPVAKPKPQSRNFK
                     AEREARAKAEAEKRQHNGDHRKNNRHNDTRSDDRRHQGQKRSNGNRNDNRQGQQNNRN
                     KNDGRYADHKQKPQTRPQQPAGNRIDFKARAAALKAEQNAEYSRHSEQRFREEQEAKR
                     QAAKEQELAKAAALKAQEEAQKAKEKLASKPVAKVKEIVNKVAATPSQTADSRRKKQT
                     RSDKSRQFSNENEDGQKQTKNKKNWNNQNQVRNQRNSNWNHNKKNKKGKTNGAPKPVT
                     ERKFHELPKEFEYTEGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGDTIELL
                     MVDYGIEAHAKVEVDEADIERFFADEDYLNPDNLTERPPVVTIMGHVDHGKTTLLDTL
                     RNSRVATGEAGGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMRARGASVTDITILIV
                     AADDGVMPQTVEAINHSKAAGVPIIVAINKIDKPGANPERVISELAEHGVISTAWGGE
                     SEFVEISAKFGKNIQELLETVLLVAEMEELKADADVRAIGTVIEARLDKGKGAVATLL
                     VQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGDHFAV
                     YADEKAARAAGEERAKRALLKQRQNTQRVSLENLFDTLKAGEVKSVNVIIKADVQGSV
                     EALVASLLKIDVEGVKVNVVHSAVGAINESDVTLAEASNAVIIGFNVRPTPQARQQAD
                     ADDVEIRQHSIIYKVIEEVEEAMKGKLDPEYQEKILGEAIIRETFKVSKVGTIGGFMV
                     INGKVTRDSSVRVIRDGVVIFDGKLASLKHYKDDVKEVGNAQEGGLMIENYNDLKEDD
                     TIEAYIMEEIKRK"
     gene            7908..8276
                     /gene="rbfA"
                     /locus_tag="VBE22_000921"
     CDS             7908..8276
                     /gene="rbfA"
                     /locus_tag="VBE22_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEO7202391.1"
                     /translation="MKKGLIMANHRIDRVGMEIKREVNEILRFRVNDPRVQDVTITDV
                     QMLGDLSMAKVFYTIHSTLASDNQKAQIGLEKATGTIKRELGKNLTMYKIPDLQFVKD
                     ESIEYGNKIDEMLRNLDKKD"
     gene            complement(8360..9364)
                     /locus_tag="VBE22_000922"
     CDS             complement(8360..9364)
                     /locus_tag="VBE22_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7202392.1"
                     /translation="MKPSFKKLLLLFSIITILSIACTPHAKASGRSWKSWFIEQYFWL
                     KRDKSYYKVQDESSFQKYLNASREQSDKGYYLDPNSVNGGLVQERLFDMQVYSWNDNG
                     KANQKTIFYLAGGSYLNNPTPYHISMLKTLSTSLDAKIILPIYPKTPRYTYDYAIPRL
                     VNPYRHFHEKNANLTLMGDSAGGGLALGLAHALSHQSGQEAIPQPKNIILLSPWLDVT
                     MKHPEIPKYEDTDPILSAWGLARVGEIWANGSNNTNHTYVSPKNAPATKLAPITLFTG
                     TREIFFPDIRDYAAQLQAANHPVNYIAQEGMNHVYPIYPIEEAKTAQYQMIDIINKTP
                     "
     gene            9527..9943
                     /locus_tag="VBE22_000923"
     CDS             9527..9943
                     /locus_tag="VBE22_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEO7202393.1"
                     /translation="MTISNAEWEIMRVVWAQQNTTSNEILAVLLEKYDWTPSTVKTLL
                     RRLLDKGYVSREKMGKGFSYSPLIDEDLAMMSEVDSVLQKVCQTKHVAIVRHLLESIP
                     MTEKDRLNLQASLEAKKGKTLERVACNCIPGQCQCH"
     gene            9956..12190
                     /locus_tag="VBE22_000924"
     CDS             9956..12190
                     /locus_tag="VBE22_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEO7202394.1"
                     /translation="MANRKETFLIDGMTCASCALTIEKAVNKLDHVDSAVVNLATEKM
                     TVTFDDTTLSPNVIEECVSETGYEASLFKEETSKSQSERHQLAIEKMWHRFWMSAVAT
                     IPLLYISMGPMINLWLPSFLMPDKGPLNYGVIQLLLTLPVMYFGRIFYQNGFKALFKR
                     HPNMDSLVAIATTAAFIYSLYGLYEILQGDIHYAHQLYFESVAVILTLITLGKYFEIL
                     SKGRTSASIEKLLTLSAKEARVIKDGEDYMVPLDKVKIGETILVKPGEKIPLDGHVVA
                     GESSIDESMLTGESIPVEKKVGSKVYGASINGQGSLTIFVEKEAGGSLLSQIINLVEV
                     AQASKAPIANLADKVSGVFVPFVIVIAILSGLSWYLILGQSFAFSLKIMIAVLVIACP
                     CALGLATPTAIMVASGKAAENGILFKGGEVLEKAHHIDTIVFDKTGTLTKGKPEVVAI
                     KTYGVDKEEFLGQVASVEKLSNHPLSQTIVNKAKEKELPLREVMAFKNILGYGLSAII
                     NGKTMLVGNANLMTKNDVNLDLAKADIEIAQEEAQTVVYVSENGVLSGLITLTDQLKT
                     DSQETVKQLQRLGFNLVLLTGDNKASADAIAQKLGITTVVSEVLPDQKANVILELKEK
                     GGQIAMVGDGINDAPALASSDVGISMSSGTDIAIESADIVLMKPELTDLLKAMTISKQ
                     TIQIIKENLFWAFFYNVLAIPVAMGVLHLFGGPLLNPMLAGLAMAFSSVSVVLNALRL
                     KVLK"
     gene            12231..12437
                     /locus_tag="VBE22_000925"
     CDS             12231..12437
                     /locus_tag="VBE22_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEO7202395.1"
                     /translation="MKHTYRVSGMKCDGCAKTVSDKLSSVMGVDEVNVDLTKNQVVVS
                     GKTFKCLLKRSLKDTKYSLEEDIN"
     gene            12547..13161
                     /locus_tag="VBE22_000926"
     CDS             12547..13161
                     /locus_tag="VBE22_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEO7202396.1"
                     /translation="MSIDIWNILSLVGTVAFASSGAIVAIEEEFDVLGLFILGFVTAF
                     GGGAIRNVLIGLPIETLWSQGIAFYAAAAAILFIMIFPNLLSGKGRDAEVVSDAIGLA
                     AFSVQGALYATQSHQPLSAVIVAAVLTGAGGGIVRDVLAGRKPGVLRSEIYAGWSILV
                     GIILYFKIAKTTTDYYLLVLVVTSLRMLGYKKQWHLPVVRPRKK"
     gene            13176..13988
                     /locus_tag="VBE22_000927"
     CDS             13176..13988
                     /locus_tag="VBE22_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7202397.1"
                     /translation="MIKLIAIDMDGTLLNDEKKIPKENIQAIKEATQAGIKIVLCTGR
                     PMSGILPYFNELGLTKEEYIIMNNGCSTYSTKDWQLIDSATLTHDELIFLEEVVKEFP
                     NVCLTLTAENTFYAVGEEVPEIVAYDADLVFTKAKSISLDALRNQEEIVFQAMYMGLD
                     ADVTAFQEAVEEALISKFSGVRSQDYIYEIMPQGVTKARGLKSLIAKLGLDINQVMAI
                     GDAPNDIELLDLVPNSVAMGNASDEIKSRCKYITVDNNQAGVAKAIYDYALR"
     gene            14101..16743
                     /gene="polA"
                     /locus_tag="VBE22_000928"
     CDS             14101..16743
                     /gene="polA"
                     /locus_tag="VBE22_000928"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEO7202398.1"
                     /translation="MTNKNKLLLIDGSSVAFRAFFALYNQIDRFKNNSGLHTNAIYGF
                     HLMLNHILGRVQPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPYIRQQLD
                     VLGIKHYELEHYEADDIIGTLAKQAEASKEHFDITVVSGDKDLIQLTDTNTVVEISKK
                     GVAEFEEFTPAYLMEKMGITPSQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLSEYGS
                     LEGIYENIEAMKQSKMKENLINDKEQAFLSKTLATINIASPITIGLEDILYSGPQDIK
                     ALSQFYDEMDFKQFKAALGEETSQEDFEVDFTEVEQLKPEMFSDNDFYYFEMLGDNYH
                     VEDLIGIAWGNSDTIYATSDVSLLQEALFKKALSKPIKTYDFKRSKVLLNRFNIDLPE
                     PAFDTRLAKYLLSTTEDNLVSTIARLYTNLPLDTDDAVYGKGAKRAIPEKTRFLEHLA
                     KKVKVLVDSEANIMQQLKANEQEELLFEMEQPLANVLAKMEIRGIKVKKNTLNEMAIE
                     NQKVIETLTQEIYELAGQEFNINSPKQLGKLLFETLGLPVEMTKKTKTGYSTAVDVLE
                     RLAPISPLVTKILEYRQITKLQSTYIIGLQDYILEDGKIHTRYVQDLTQTGRLSSSDP
                     NLQNIPVRLEQGRLIRKAFVPSEDNAVLLSSDYSQIELRVLAHISKDEHLIAAFKEGA
                     DIHTSTAMRVFGIEKPENVTPNDRRNAKAVNFGIVYGISDFGLSHNLGIPRKLAKQYI
                     DTYFERYPGIKNYMETVVREAKDKGYVETLFHRRRSLPDINSRNFNIRQFAERTAINS
                     PIQGSAADILKIAMINLDRVLYKGGYKSKMLLQVHDEIVLEVPNEEIGAIKELVTKTM
                     ESAISLSVPLIADENAGETWYEAK"
     gene            16773..17213
                     /locus_tag="VBE22_000929"
     CDS             16773..17213
                     /locus_tag="VBE22_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEO7202399.1"
                     /translation="MVYHFQNPSDFMLKNYLTKAKTIAVVGLSDRQETAAYQVSKIMQ
                     EAGYQIIPVNPKNAGQKILGQMTYASLKDVTEHIDIVNIFRRSEYLPDIAREFLEVDA
                     DIFWAQLGLESQEAETILKQAGHKQIVMNKCLKVEYQKLHSETN"
     gene            17295..17774
                     /gene="perR"
                     /locus_tag="VBE22_000930"
     CDS             17295..17774
                     /gene="perR"
                     /locus_tag="VBE22_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEO7202400.1"
                     /translation="MDSHSHGHRPLDAYENVLEHLREKRIRITETRKAIISYMVNSRE
                     HPSAEKIYNDLLPEYPNMSLATVYNNLKVLVDEGFVTELKLCNYSTTYYDFMGHQHLN
                     IACEDCGKIVDFVDVDLLDISREAHQQTGFEVTRVQLVAYGICPECQRKRKDKYKKD"
     gene            17927..19042
                     /locus_tag="VBE22_000931"
     CDS             17927..19042
                     /locus_tag="VBE22_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEO7202401.1"
                     /translation="MKISQYNKWSIRRLKVGAASVMIASGSIVALGQSHIVSADEMSQ
                     PKTTITAPTANTSTNVESSTDKALSKVTTMETSSEMPKMQNMAKVEKTSDKPMMVATS
                     VRKMMATPTPVAMTKTTSVDEVKKSTDTAFKQTVDVPAHYVNAAKGNGPFLAGVNQTI
                     PYEAFGGDGMLTRLILKASEGAKWSDNGVDKNSPLLPLKDLTKGKYFYQVSLNGNTAG
                     KKGQALLDQIKANGSHTYQATITIYGTKDGKVDMNTILGQKTVMIHINVAKKDMNSTS
                     MMMKKDKMTMPMKKEMTSSKINTGMMMSNNKMSANMQMSSQAKSNDKAGKKMSMMSKN
                     LPNTGETKQQNVGVLGMLSLAFATGLTALGLKKSKQR"
     gene            19155..19841
                     /locus_tag="VBE22_000932"
     CDS             19155..19841
                     /locus_tag="VBE22_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7202402.1"
                     /translation="MTQKLLLVDDEFEIIDINRRYLEQAGYEVSVAADGIEALKEVDE
                     NRFDLIISDIMMPKMDGYDFISEVLVREPNQPFLFITAKVSEPDKIYSLSMGADDFIS
                     KPFSPRELVLRVKNILRRIYGNHQQSEVLTIGDLVIDQKQRLVMVDCNTISLTNKSFD
                     LLWILANHLNRVFSKTELYERVWGEEFLDDTNTLNVHIHALRNDLAKFSTDNTPTIKT
                     VWGLGYKLEE"
     gene            19843..>20336
                     /locus_tag="VBE22_000933"
     CDS             19843..>20336
                     /locus_tag="VBE22_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7202403.1"
                     /translation="MKLKYYIVIGYLISMLITVAGVFFGLNHMLIETRGVYYILSVTI
                     IACIVGGIVNLFLLSSVFTSLKKLKQKMKDISQRRFDTKAQICSPQEFKDLETAFNQM
                     SSELESTFKSLNESEREKTMMIAQLSHDIKTPITSIQSTVEGILDGIISEEEVNYYLN
                     TISR"
BASE COUNT         6894 a         3122 c         4157 g         6163 t
ORIGIN      
        1 agaagcaata aaggaaatta taaattctgc taaaaaacgg acttatatct tttggttggt
       61 tattcaaacc ttatttttag tattaatatc accaattttg ataaagttag gcttgtcagt
      121 ttttatgatt acattgttaa tttttggtct aggaataatc aaatggttgg tgataactta
      181 caaggtaaag gtcttttata acaatcaaaa tttgaactgg gatgcagcta taaatcatga
      241 gcaggaaaga aaacagagta tattaaaatt tttctctttg tttacaaatg ttaagggaat
      301 ttcgacaagt gtaaaacgtc gttcattttt agatgttatt ttaaaactta tttctaaaac
      361 gccatcgcgt ttatggacta acttatttgt gagggcattt ctaagaagct cagattatct
      421 tggtttaaca ataaggttgg taactttaaa tatcttatca gtcatctttg ttaacgaaac
      481 ctacctcgcc cttgctctgg catttgtatt taactactta ttattgttcc aattattagc
      541 tttaggtcac cattttgatt atcaatacat gaatcagctc tatccagtta gattaaatgc
      601 taaagcaagt cagctaaaag gttttctcag agtattatca tatgctgtga cagtaattga
      661 tatcatactt ataagagaat tggaacctgt tatattattg atagttttga tgttaattgt
      721 gacggaatat tacatccctt acaaaataaa gaagatgatt gactaaagcc tataaaacaa
      781 gtaaaataag aggaaacgac taaatgaaaa ggagggactg ctggtgacaa tatcaaatca
      841 agaattgacg cttactccct taagaggaaa aagtggcaaa gcctatattg gtacttatcc
      901 aaatggtgaa cgtgtctttg tcaaatataa tacgacacct attttgccag ccttagctaa
      961 ggaacagatt gcgccacaac tactgtgggc acgccgtaca agtaatggcg atatgatgag
     1021 tgctcaagaa tggttagatg gtcgaacatt gactaaagaa gatatgggta gtaaacagat
     1081 tattcatatt ttattgcgcc tgcacaagtc tcgtccctta gtaaatcaac tgttacagtt
     1141 agggtataaa attgaaaacc cttatgattt gttgatggat tgggaaaaac aaactcctat
     1201 tcaaatccga gaaaacactt atttgcaatc tattgtgact gagttaaagc gtagtttacc
     1261 tgagtttcga acagaggttg caacaatcgt tcacggagat atcaaacata gcaactgggt
     1321 tattacaaca agtggtttaa tttatttagt tgattgggat tctgttcgtt taacagatcg
     1381 catgtatgac gttgcatata ttttaagtca ttatattcca caaaaacatt ggaaagattg
     1441 gctgtcttac tacggttata aagataatga gaaagtctgg agcaaaatta tttggtatgg
     1501 gcaattttca tatttatcac agataatcaa atgttttgat aaacgagata tggagcatgt
     1561 caatcaagaa atttatgaat tacgaaagtt tagagagtta attaaaaagc ataatgcgag
     1621 ttagaaaacg taagggtgca gaggaacact tagaaaataa tcctcactat gttatttcaa
     1681 atccagaaga agccaaaggg cgttggcatg agatttttgg aaataataac cctattcaca
     1741 tagaagttgg ttccggaaaa ggggctttta tcactggtat ggcagagcaa aatccagata
     1801 tcaattatat tggaattgat attcaattgt cagtgctttc ctatgctctg gataaagttc
     1861 tagatagcgg tgctaagaat atcaaattac tattagttga tggttctagt ctatcaaact
     1921 actttgatac tggcgaagtt gatttgatgt atcttaactt tagtgatcca tggcctaaga
     1981 aaaagcatga gaaacgtcga ttgacctata agactttctt ggatacctat aaagacatac
     2041 ttcctgagca gggggaaatt cattttaaaa cagataatag aggtttattt gaatatagtc
     2101 ttgctagctt ttctcaatac ggtatgactt taaaacaagt ttggttagat ttacatgcaa
     2161 gtgattacca gcaaaatatc atgactgagt acgaacgcaa attttcaaat aaaggacagg
     2221 ttatctatcg tgtagaagca cgattttaga ataagtaaat agtaagtggg tagttatttg
     2281 ctactccttt atggagaggt cgcatagtgg ctgagtgcgc acgcttggaa agcgtgtagt
     2341 ccttaatggg gctcgggggt tcgaatcccc tcctctcctt taatcttgtt aatgtatcag
     2401 tgtttattaa gtgttcacct ttttcactcg tcaaaagtcc aagtttcgaa aaatttcttt
     2461 tattttatca tcgtttttct ttgagcttga agtattagca taaagttttc taagctctta
     2521 caaagaactg aggacaaaat tttaattttt tgatataaga ttagtgtacg gtgatgctaa
     2581 tttaaagtaa atgattgcaa taatcgtatt ttgtgctata atagtattaa ataatacaga
     2641 gaggggcgga gttttacttc gcctcttatg tgtgtgattc atagaattta tagtcattat
     2701 aagttctaaa gcaagttaga aagaagaggt agctgttatc gcaaatcaaa ccattgttga
     2761 tattgttact caagtcgtaa ctcctgctat tcaagcaccg tttgaattag tagatgtaga
     2821 atacgaaaaa atgggtggcg actatgtgct cagtatttta attgataaac ccggaggcat
     2881 tacggttgaa gatacagcac aattgacaga cgttgtaagt cccttacttg acacaattca
     2941 accagatcca tttccagaac agtatatgct tgaagtctca agtcctgggt tggaaaggcc
     3001 acttaaaaca gcagaagcat tatcaaatgc tgttgggtct tatatcaatg taagtctgta
     3061 taaatccatt gataaagtta aaatatttga aggtgactta ttatcatttg atggtgaaac
     3121 cttgacaatt gattatatgg acaaaacacg tcataaaact gtagatattc cctatcagac
     3181 ggttgcaaaa gcccgtcttg cggttaaact ttaagaaagg atgattcttg cgaagcaaga
     3241 atggacacta tgagcaaaga aatgctagaa gccttccgta ttttggaaga agaaaaacac
     3301 attaataaag aagatattat tgatgcagtt actgagtcac taaaatctgc ctataagcgt
     3361 cgttatggtc aatcagaatc atgcgttatt gaatttaacg agaagaaagc agactttacc
     3421 gtctatactg ttcgtgaagt tgttgatgaa gtttttgata gccgtttgga aattagccta
     3481 aaagacgcac ttgctattag ctcagcgtat gaacttggtg ataagattcg ctttgaggaa
     3541 tctgttacag aatttggacg cgtggcagct cagtcagcaa aacaaactat tatggaaaaa
     3601 atgcgtcgtc aaatgcgtga agtaaccttc aatgaataca aacaacacga aggtgaaatt
     3661 atgactggta ctgttgaacg atttgaccaa cgttttatct atgtcaatct cggttcactt
     3721 gaggcgcaat tatcgcatca agatcagatt cctggagaga gctttaaatc tcatgatatg
     3781 attgacgttt atgtctataa agtggagaat aatcctaaag gagtcaacgt ttttgttagc
     3841 cgtagtcatc cagagtttat caaacgcatt atggagcgag aaattccaga agtatttgat
     3901 ggaactgttg agattatgag cgtctctcgt gaggctggag atcgtactaa agttgctgta
     3961 cgcagtcata attcaaatgt tgatgcgatt ggaacaattg ttggacgcgg tggaagtaac
     4021 attaaaaaag ttatcagtaa tttccatcct aaacgcgttg atgctaaaac aggattggag
     4081 atcccggttg aagaaaacat tgatgttatt caatgggttg aagatccagc ggaattcatt
     4141 tacaatgcta ttgcaccagc cgaagttgat atggtacttt ttgatgatga agatacaaaa
     4201 cgtgctactg tagttgttcc agatagcaag ttatcgctag ctattggtcg ccgaggacaa
     4261 aatgtgcgtt tagcagctca tttgactggc tatcgtattg atattaaatc tgcaagtgaa
     4321 tacgaaaaaa tggaagcgca agaacttcaa actgaggaag tagctcaaga atctgaagtt
     4381 atttcagata aggcagaata atataaatga gagaggtgtt ttatggctaa aacaaaaaaa
     4441 atacctttaa gaaaatcagt tgtttctggt gaagtgatcg ataagcgaga tcttttacga
     4501 attgttaaaa ataaagaagg acaggttttt atagatccta ccggaaagca aaatggtcga
     4561 ggtgcctaca taaaattgga caatgatgaa gctatcctag cgaaaaagaa acgagtgttt
     4621 gatcgcagtt tttcaatgga agtttctgat gaattctatg atgaactatt ggcatatgtc
     4681 gaccataagg tcaaaagaag agagttaggc cttgaataat tcagaaaaag tattaaatct
     4741 cattgggtta gcacagcgtg cggggcgcct tattacaggt gaagaattgg ttattaaagc
     4801 aattcaaaat caacaagtat cgctaatctt tctagcaaat gatgctggcc caaatttgac
     4861 gaaaaaagta actgataaaa gtaattacta caaaacagaa gtctccacag tgttttcaac
     4921 actggaatta agtgatgcgt taggcaaacc aagaaaagtg gttgctgtcg cagacgctgg
     4981 attttcaaag aaaatgagga ctcttatggc ataacagaat aggaggacat caattgtcaa
     5041 agaaaagatt acacgaaatc gctaaggaaa ttggaaaaac gagtaaggaa gtggttgagc
     5101 aagctcaatc gcttggctta cctgtaaaaa gtcatgcttc tagcgttgaa gaaaatgatg
     5161 ctacacgtat tgttgagagt tttagctctt ctaaaactaa agctccaaca aattctgttc
     5221 aaactaatca aggtgtgaaa acagaatcta aaactgttga aaccaaacaa ggcttgtccg
     5281 atgataaacc ttcaactcaa cctgtagcta aaccgaaacc acaaagtcgt aactttaagg
     5341 cggagcgcga ggctcgtgct aaagctgaag cggagaagcg tcaacataat ggggatcacc
     5401 gtaagaataa tcgccataat gatacacgct ctgacgacag acgccatcaa ggtcaaaagc
     5461 gttcaaatgg taatcgtaat gataaccgac aaggtcagca aaataaccgt aataaaaatg
     5521 atgggcgtta tgctgatcat aaacagaaac cccaaacgcg tcctcagcaa cctgcaggca
     5581 atcgcattga ctttaaagct cgagcagcag ctttgaaagc tgagcaaaat gctgaatact
     5641 cacgtcatag tgagcagcgt ttccgtgaag agcaagaagc caaacgtcaa gcagctaagg
     5701 agcaagaact agctaaagca gcagcgttaa aagcgcaaga agaagctcaa aaagctaagg
     5761 aaaaacttgc ttctaaacct gttgctaagg taaaagaaat tgtcaataaa gtagccgcta
     5821 cgccatcaca gacagcagat agtcgtcgta aaaaacaaac tcgttctgat aagtctcgtc
     5881 aattttcaaa tgaaaatgaa gatggtcaaa agcaaacaaa aaataagaaa aactggaata
     5941 atcaaaacca agtgagaaat caaagaaata gtaactggaa tcataataag aaaaataaaa
     6001 aaggtaagac taatggagct ccaaaaccag taacagagcg taaattccat gaattaccaa
     6061 aagaatttga atatactgag ggcatgaccg ttgcggaaat tgcaaaacgt attaaacgcg
     6121 aaccagcaga aattgttaaa aaactcttta tgatgggagt tatggcaacg cagaatcaat
     6181 cattagatgg agacactatt gagctattga tggttgacta cgggattgaa gcacatgcta
     6241 aggttgaagt tgatgaagct gatattgaac gcttctttgc agatgaggat taccttaatc
     6301 ctgataactt aacggaacgt ccgcctgttg ttactatcat gggacacgtt gatcatggta
     6361 aaacaactct tcttgatact ctacgtaatt cacgtgtcgc tactggcgaa gctggtggaa
     6421 ttactcaaca tattggtgct tatcagattg aagaagctgg caagaaaatt actttcttgg
     6481 atacaccagg gcatgcggcc tttacttcta tgcgtgcgcg tggtgcatca gtaactgata
     6541 ttactatcct tatcgttgcc gctgatgatg gtgttatgcc gcagacagtt gaggcaatta
     6601 atcactctaa agctgcgggt gtcccaatta ttgttgctat caacaaaatt gataaacctg
     6661 gtgcaaatcc agaacgcgtt atcagtgaat tagctgagca tggtgtcatc tctactgcat
     6721 ggggtggtga gtctgaattt gttgaaattt cagctaagtt tggtaaaaat attcaagaat
     6781 tacttgagac agttctttta gttgctgaaa tggaagaatt aaaagcagat gcagatgttc
     6841 gtgctattgg tactgttatt gaagcccgtc ttgataaagg taagggagcc gttgcaacgc
     6901 ttcttgtcca acaaggtact ttaaatgtac aagatccaat cgttgttggt aatacatttg
     6961 gacgtgttcg tgcgatgact aatgatcttg ggcgtcgtgt caaagttgcg ggtccatcta
     7021 caccagtttc tattactggt ttgaacgaag ctccaatggc tggtgatcat tttgctgttt
     7081 atgcagatga gaaagctgcg cgtgctgcag gtgaagaacg tgcaaaacgt gctctcttaa
     7141 aacaacgcca aaacacgcaa cgtgttagcc tcgagaactt atttgatacc cttaaagctg
     7201 gtgaagttaa gtcagttaat gttattatca aagctgatgt gcaaggttct gtagaagcac
     7261 ttgtagcttc tctccttaag attgatgttg aaggagtaaa agttaatgtt gttcactcag
     7321 cagttggtgc tattaatgag tctgatgtaa ctcttgctga agcatcaaat gctgttatta
     7381 taggatttaa cgtacgccct actccacaag ctcgtcaaca agccgatgct gatgatgtcg
     7441 aaattcgtca acatagcatt atctataaag ttatcgaaga agttgaagaa gcgatgaaag
     7501 gtaaacttga tcctgaatac caagaaaaaa tccttggtga agccattatt cgtgagacct
     7561 ttaaagtttc aaaagtggga actattggtg gatttatggt tatcaatggt aaagttactc
     7621 gtgattcaag tgttcgtgtt atccgtgatg gtgttgtcat ttttgatggt aaattagcaa
     7681 gtcttaaaca ctataaagat gatgttaaag aagtcggcaa tgctcaagaa ggtggtctaa
     7741 tgattgagaa ttataacgat ctcaaagaag atgacactat tgaagcatac attatggaag
     7801 aaattaaacg taaataaccc ctatgagaaa cccaacttgt atctttagtt ggtagggaaa
     7861 tacaaaatta tggctaggct cgtcctagct ttcaagttat gagcacaatg aagaaaggat
     7921 taattatggc taatcatcgt attgatcgcg ttggaatgga aattaaacgt gaagtcaatg
     7981 agattttgcg tttccgagtt aatgatccac gtgtacaaga tgtcacaatt actgatgttc
     8041 agatgttggg tgacctttca atggctaaag tattttatac tattcattcg acacttgctt
     8101 ctgataatca aaaagcacaa attggtttag aaaaagcaac tggtactatt aagcgtgaac
     8161 ttggcaaaaa ccttactatg tataaaatac cagatttaca atttgttaaa gatgaatcta
     8221 ttgaatacgg aaataagatt gatgaaatgt tgcgcaatct agataaaaaa gattagaatc
     8281 aagattaaga ataataaaaa ataaaccatg tcaaaatcat ggtttatttt ttatagcttt
     8341 aaagttgaga tagctagtat taaggagttt tattgatgat atcaatcatt tgatactgtg
     8401 ctgttttagc ttcttcaata gggtaaattg gataaacatg attcattccc tcttgtgcaa
     8461 tatagttaac ggggtgatta gctgcttgga gttgtgcagc ataatcgcga atatctggga
     8521 agaatatttc tcgtgttcca gtaaataacg taataggagc gagtttagta gcaggggcat
     8581 ttttaggact aacatatgta tggttagtat tattagaacc atttgcccaa atttcaccta
     8641 cacgtgcgag accccaagca gataggatag gatcggtatc ttcgtatttg ggaatttctg
     8701 gatgtttcat tgtcacatct agccatggag ataagaggat aatattttta ggttgaggaa
     8761 tagcctcttg ccctgattgg tgagacaatg catgcgctaa tcctagtgct aatcctccgc
     8821 cagctgaatc acccatcaaa gtgagattag cgtttttttc atgaaagtgt ctataagggt
     8881 taaccaatct agggatagca taatcatagg tataacgagg agtttttgga taaattggta
     8941 aaataatttt tgcatcaaga ctagttgaga gcgtcttaag catgctgata tggtaaggcg
     9001 ttggattatt taagtaagag ccaccagcta aatagaaaat ggtcttttga ttagctttac
     9061 cgttgtcatt ccaagaatag acttgcatat caaaaagtct ctcttgaaca agccctccat
     9121 taacagagtt tgggtctaag taataaccct tatcagattg ttcacggcta gcatttaagt
     9181 acttttgaaa tgagctttca tcttgaactt tataataact tttatcgcgc tttagccaaa
     9241 agtattgttc gataaaccaa cttttccatg aacgcccact tgcttttgca tgtggtgtac
     9301 aggcgatgga taaaatggtg ataatactaa atagtagcaa tagttttttg aaggacggtt
     9361 tcaaggtatt ctccttaata taatgttatt tttgtgtaat attattataa taaaaccaac
     9421 atctttgtca atagaataaa gaaagatgag aaatgttttt cggtttacaa atgtagattt
     9481 atttgctaaa attggtttac aaacgtaaat cgaaagaggt gagaaaatga ctatttcaaa
     9541 cgcagaatgg gaaattatga gagtggtttg ggcacagcag aatacaacca gtaatgagat
     9601 actagcagtt ttactagaga agtatgattg gactccttca actgtcaaaa ccttattaag
     9661 aaggctctta gataaaggct acgtgagcag agaaaaaatg ggaaaaggat ttagctattc
     9721 tcctttaatt gatgaagatt tagctatgat gtcagaggta gatagtgtat tgcagaaagt
     9781 ttgccagacc aaacatgttg ctattgttag acacttattg gaaagtatac caatgacaga
     9841 gaaggatcga ttaaatttgc aagcatcact agaagctaag aaaggcaaaa cacttgaacg
     9901 tgtagcttgt aattgcattc ctggtcagtg tcaatgccat tagaaaggag aaaatatggc
     9961 taatagaaaa gaaacatttt tgattgatgg gatgacttgt gcatcttgtg ctttaacgat
    10021 agaaaaagct gtcaacaagt tagaccatgt ggatagtgca gtagtaaatt tagcaacaga
    10081 gaaaatgaca gttacgtttg atgatacaac cttatcgcca aacgtgattg aagagtgtgt
    10141 ttctgagact ggatacgaag catcgctttt caaagaggaa acttctaaaa gccaatctga
    10201 acggcaccaa ttagcaattg aaaagatgtg gcatcgcttt tggatgtcag cagttgcaac
    10261 tatacctctt ctctatattt ccatggggcc aatgattaac ttatggttac catcattttt
    10321 gatgccagat aagggacctc taaactatgg tgtgatacaa ttattgttaa cactaccggt
    10381 gatgtatttt gggcgtatct tttaccaaaa tggttttaaa gcacttttta aaaggcatcc
    10441 caatatggat tcattagttg ctatagccac aacagcggct ttcatttata gcttatatgg
    10501 attgtacgaa atattacagg gtgacataca ctacgctcac cagctttatt tcgaatctgt
    10561 tgcagttatt ttaacactta ttactctagg aaaatatttt gaaatattat ccaaagggcg
    10621 tacttcggcc tcaattgaaa aattattaac tttatcagca aaagaagcaa gagttatcaa
    10681 agatggtgaa gactatatgg ttcctttgga taaagttaaa ataggtgaaa caattcttgt
    10741 caaacctgga gaaaaaattc ctcttgatgg ccatgttgta gcaggagaat caagtattga
    10801 tgaatccatg ttaacaggtg aaagtattcc agttgaaaaa aaggttggta gtaaggttta
    10861 cggtgccagc attaatgggc aaggtagctt aacgattttc gttgaaaaag aagctggcgg
    10921 ttcccttcta tcacaaatta ttaacttagt agaagttgca caggcaagta aggctccaat
    10981 tgctaactta gcagacaaag tatcaggagt ctttgtccct tttgttattg tcattgcaat
    11041 tttatcaggt ttgagttggt acttaattct ggggcaaagt tttgccttct ctctgaagat
    11101 tatgattgca gtcttagtga ttgcttgtcc atgtgcttta gggttagcaa ctcctacagc
    11161 tattatggtg gctagtggta aagcagctga gaatggtatt ttatttaaag gtggtgaagt
    11221 gttggaaaag gctcaccaca ttgatacaat agtttttgat aaaacaggaa ccttgaccaa
    11281 agggaaacca gaagtagttg ctatcaaaac atatggggta gataaagagg agtttttagg
    11341 tcaagtagca tcagtagaaa aactttcaaa tcatccactt agccaaacaa ttgttaacaa
    11401 agcaaaagag aaagaacttc ccttaaggga agtcatggct tttaaaaata ttttgggtta
    11461 cggtttgagt gcaattatta atggtaaaac tatgcttgta ggtaatgcta acttaatgac
    11521 taagaacgat gttaatttag atttagcaaa ggctgatata gaaatagctc aagaagaagc
    11581 acaaactgtg gtttacgttt ccgaaaatgg agtgttgagc ggtttaataa ctttaacgga
    11641 ccagctaaag acagatagcc aagaaacagt taagcagtta caaagattag gatttaattt
    11701 agttttactt actggtgata ataaggctag tgcagatgcc atagcacaaa aattgggtat
    11761 cacgacagtt gtttcagaag ttttacctga tcagaaagca aacgttattt tagaacttaa
    11821 agaaaaaggc ggacagattg cgatggttgg tgatggcatc aatgatgctc ctgcgctagc
    11881 tagcagtgat gtaggtatct ctatgagttc tggaacagat attgctattg aatcagcaga
    11941 tatcgtgttg atgaaacctg agttaacaga tctcttaaaa gcaatgacta tcagtaaaca
    12001 aacgattcaa attattaagg aaaatttatt ttgggctttc ttttataacg ttttagcgat
    12061 tccagtggca atgggtgttt tacatctttt tggtggtccc ttactcaatc ctatgttggc
    12121 aggcttagct atggcgttta gttcagtttc agtagtttta aacgcacttc gcttaaaagt
    12181 cttaaaataa taaagataat aagactgaat ttttatgaag ggagaagaat atgaaacata
    12241 cctatcgagt tagtggcatg aaatgtgatg gttgtgccaa aacagtttct gataagttat
    12301 caagtgtgat gggtgttgac gaggttaatg ttgatttgac taaaaatcaa gttgtcgtaa
    12361 gtggaaaaac attcaaatgt cttttaaaac gttctttaaa agacacaaag tattcactag
    12421 aagaggacat aaattaataa ctaaagaagt ggttacagct tttaatttct taatttgagc
    12481 agtttctcac ttctttttta ttctaaaaat gataaactag aatagaatta gttgtgagga
    12541 gtgcagatgt caatcgatat ttggaacatt ttaagtttgg ttggaaccgt agcttttgca
    12601 agttcgggag ctattgtggc gattgaggaa gaatttgatg ttctagggct ttttatctta
    12661 ggttttgtaa cggcatttgg tggtggagct attagaaatg tactaattgg cttgcctatt
    12721 gaaacacttt ggagtcaagg aattgctttt tatgcagcag cagcagctat tttgtttatt
    12781 atgattttcc ctaacttgtt gagtggtaag ggacgggatg ccgaagttgt atcagatgct
    12841 attgggttag cagcgtttag tgttcaagga gcactatatg cgactcaatc tcatcaacct
    12901 cttagtgctg tcattgtcgc agcagtacta actggtgcag gtggtggtat tgtcagagat
    12961 gttctggctg gccgaaaacc tggggtacta agaagtgaaa tatatgcagg ttggtcaata
    13021 ttagtgggga tcattttata ttttaaaatt gctaaaacaa caactgacta ttatttgtta
    13081 gtgttagttg taactagcct aaggatgttg gggtataaga aacaatggca cttgccagtt
    13141 gtcagaccac gtaagaaatg atagaggaga aagagatgat aaaattaatt gctattgata
    13201 tggatggaac gcttttaaat gatgaaaaga aaattccaaa ggaaaatatc caagctatta
    13261 aagaagcaac tcaagcaggt attaaaattg tactgtgtac tgggcgaccg atgtcaggta
    13321 tacttcctta ttttaatgaa ctaggtttaa caaaagagga gtatattatc atgaataatg
    13381 gttgtagcac ttatagcacg aaagattggc aattgattga ttctgcaact ttaacacatg
    13441 atgaactcat ctttcttgag gaggttgtta aagaatttcc caacgtttgt ttaacgttaa
    13501 cagctgaaaa tacattttac gctgttggtg aggaagttcc tgaaattgtt gcttatgatg
    13561 ctgatttagt ttttacaaaa gcaaagtcaa taagtttaga tgctcttcgc aatcaagaag
    13621 aaattgtctt tcaagcaatg tacatgggat tagatgcaga tgtaactgct tttcaagaag
    13681 ctgttgaaga agccttaatc tcgaaattta gtggagttcg aagccaagac tatatttatg
    13741 agattatgcc tcaaggagta acaaaagcaa gaggcttaaa atccttgata gcaaaattag
    13801 gactggatat taatcaagtt atggctattg gtgatgctcc taacgatatt gaattattag
    13861 atttagttcc caatagcgtt gctatgggga atgcatcaga tgagattaaa tctagatgta
    13921 aatatataac ggtggataat aaccaagccg gggttgctaa agctatttat gactatgctt
    13981 tacgttaaaa tagatataga ataataaaag gaactttaaa cactgtttgt ttagggttct
    14041 tttgtttaat aaactaacaa aattcagaag tttacagata gcttatgcta taatagacct
    14101 atgacaaaca agaataaatt acttttaatt gatggctcat ctgtagcctt tcgtgctttt
    14161 tttgctcttt ataatcaaat tgaccgcttt aaaaacaatt ctggacttca tactaatgcc
    14221 atatatggtt tccatttgat gttgaatcat attttaggtc gtgtccagcc tagtcatatt
    14281 ttagtggctt ttgatgctgg aaaaactact ttccgtacgg aaatgtatgc agattataaa
    14341 ggtgggcgtg ctaaaactcc ggatgaattt cgtgaacaat ttccttatat tcgtcaacaa
    14401 ttagatgtct taggcatcaa acattatgaa ttggaacatt atgaagccga tgatatcatt
    14461 ggtactcttg caaaacaagc cgaagctagt aaagagcatt ttgatataac ggtggtttct
    14521 ggggataaag atttaattca attaacagac actaacacgg tagtagaaat ctctaaaaag
    14581 ggagttgctg agtttgaaga attcacgcca gcttacctta tggaaaaaat gggaattacg
    14641 ccgagtcaat ttattgattt aaaagctttg atgggagata aatcagataa tattcctggg
    14701 gttactaaaa tcggtgaaaa aactggcttg aagttgctat cagaatatgg ttccttagaa
    14761 gggatctatg aaaatattga ggctatgaaa cagtctaaga tgaaagaaaa ccttatcaat
    14821 gataaggagc aagctttctt atcaaagacc ctagctacaa tcaacatagc atcacctata
    14881 acaatcggct tagaagatat cctttatagt ggaccgcaag atataaaagc cttgtctcaa
    14941 ttttatgatg agatggattt taaacagttt aaagcagctc tgggtgagga gacaagtcag
    15001 gaagattttg aggttgactt tactgaagtg gagcaattaa aaccagaaat gttctccgat
    15061 aatgattttt attactttga gatgttaggt gataactacc acgtagaaga tttaattggg
    15121 atagcttggg gaaatagcga tacaatctac gctactagtg atgtatcgtt attgcaagaa
    15181 gctttattta aaaaagcttt atcaaaacct atcaaaactt atgattttaa aagaagtaag
    15241 gtccttttaa atcgttttaa tattgattta ccagagccag cctttgatac tagattggcc
    15301 aagtaccttt tgtctactac tgaggataat ctagtatcaa caattgctag gttatataca
    15361 aatctgccac tggatactga tgatgctgtt tatgggaaag gtgctaaacg cgctatacca
    15421 gaaaaaacac gatttcttga gcatctagct aaaaaagtaa aagttttagt ggattctgag
    15481 gctaatatta tgcaacagct taaagctaat gagcaagaag aattgctgtt tgaaatggag
    15541 caacctttag cgaatgtttt agctaaaatg gaaattcgag gtattaaggt taagaaaaat
    15601 accttgaatg aaatggctat tgaaaatcaa aaagtcatcg aaaccttaac acaagagatc
    15661 tatgaattgg ctgggcaaga gtttaatatt aattctccaa aacagttagg aaagttgttg
    15721 tttgaaacgt taggtcttcc tgtagaaatg actaaaaaaa caaaaacagg ttattcaact
    15781 gcagtagatg tattagagcg cttagctccg atttctcctc ttgtaactaa aattttagag
    15841 taccgtcaaa tcactaaatt gcaatcaacc tatattattg gtttacaaga ttatatttta
    15901 gaagatggta aaattcatac acgttatgtt caagatttga ctcagacagg tcgtctttca
    15961 agttctgacc caaaccttca aaatatacca gttagactag agcaaggacg gcttatccga
    16021 aaggcttttg ttccatcaga agacaatgct gttttattga gttcagatta ttctcaaata
    16081 gaattgcgcg tgttagcaca tatttcaaaa gacgaacatt tgatcgctgc ctttaaagag
    16141 ggagcagata ttcatacttc aacagctatg cgtgtttttg gtatcgaaaa acctgagaat
    16201 gtcactccca atgatcgtcg aaatgctaag gctgttaact ttggaattgt ttatggtatt
    16261 tcagattttg gtctatcgca taatttagga atacctcgta aactagcgaa gcaatatatt
    16321 gacacctatt ttgagcgtta tcctggaata aaaaactata tggaaacagt tgttcgtgaa
    16381 gcaaaagata agggttacgt tgaaacactt ttccatcgtc gtcgttcact accagatatt
    16441 aattctcgta attttaacat tcgccaattt gcagagcgca cagcaatcaa ttcaccaatc
    16501 caaggaagtg cagctgatat tcttaaaatt gcaatgataa atttagatag ggtactttat
    16561 aaaggtggtt ataaatcaaa aatgctgttg caagttcatg atgaaattgt acttgaagtc
    16621 cctaacgagg agataggagc aatcaaggag ttggttacaa aaacaatgga gtcagcaatt
    16681 agtttatcgg tacctttgat tgcagatgag aacgcaggtg agacttggta tgaagcgaag
    16741 tagtggacaa catacgtaaa ggagtcatta atatggttta tcattttcaa aatccaagtg
    16801 attttatgtt aaaaaattat ttaacaaaag caaagaccat cgcagttgta ggattgtcag
    16861 accgtcaaga aactgcagct tatcaagtat caaaaataat gcaagaagca ggttatcaaa
    16921 ttattccagt taatcctaaa aatgcaggac agaaaatact tgggcaaatg acatatgcct
    16981 cacttaagga tgtcactgaa catattgata ttgttaatat ttttcgacgt agcgagtatt
    17041 tacctgatat tgcaagagaa tttttagaag tagacgctga tattttctgg gctcagcttg
    17101 gacttgaaag tcaagaagca gagacaattc taaagcaagc aggacacaaa cagattgtca
    17161 tgaataaatg tctgaaagtt gaataccaaa aattacactc agaaactaac taattaccgt
    17221 gtttaatagg caaataatat aaattatgct ataataacca tgctaaatta aagttcgtgc
    17281 tggaaaggag tcctatggac agtcattcac atggtcatag acctctcgat gcttatgaaa
    17341 acgtcctaga gcatctccgt gaaaaacgta ttcgaattac tgagactcga aaagctatta
    17401 tttcctatat ggttaatagt cgtgagcatc cgagtgcaga aaagatttac aatgatttat
    17461 taccagaata tcctaacatg agcttggcaa cagtttacaa taaccttaag gtccttgtgg
    17521 atgaggggtt tgtgacagag ctaaaacttt gcaattatag tacaacttat tatgatttta
    17581 tggggcatca gcacctaaac attgcctgtg aagattgtgg gaaaatcgtt gattttgtcg
    17641 atgttgactt attggatatt tctagagaag cacaccaaca aacaggtttt gaagtgacta
    17701 gagttcagtt agtagcttat ggtatctgtc cggagtgtca gaggaagcgt aaggataaat
    17761 ataaaaaaga ttgaaacgta attgttcaat ctttttttgt ttctagacta agagaaaaag
    17821 tttaagtttt actaagtaat aattaagaaa taatttataa ttgcttaaaa aatgatttat
    17881 tttttgttgg tatactctca ttaacaaaaa gaaagaggaa tcccttatga aaatatcaca
    17941 atacaataaa tggtcaattc gccgattgaa agttggagct gcatctgtaa tgattgctag
    18001 tggaagcatt gttgctttag ggcaatctca tattgtttca gcagatgaga tgtcccaacc
    18061 taaaacaacc attacagccc caacagctaa cacttctaca aatgtagaaa gtagtactga
    18121 taaggcatta tcaaaagtta ctacgatgga gacaagttca gaaatgccta aaatgcaaaa
    18181 tatggcaaaa gttgaaaaaa cgagtgacaa acctatgatg gtggctacct ctgttagaaa
    18241 aatgatggca acgccgacac ctgtagcgat gaccaaaacg actagtgtag atgaggtaaa
    18301 gaaatcaact gatactgctt ttaaacagac agtagacgtt ccggctcatt atgttaatgc
    18361 agcaaaaggg aatggcccat ttctagcagg tgtaaatcaa acgattccat atgaagcgtt
    18421 tggtggagac ggcatgttaa cgcgtttaat ccttaaagct tcagagggag caaaatggtc
    18481 agacaatggt gttgataaaa atagtccatt attaccgtta aaagatttaa ctaagggtaa
    18541 atacttctac caagtctctc ttaatggcaa tacagccggt aaaaaaggcc aagctctctt
    18601 agatcaaatt aaggcaaatg gaagccatac ttatcaagca actatcacta tttatggtac
    18661 gaaagatggc aaagttgata tgaatactat tttaggacaa aagacagtga tgattcatat
    18721 taatgtcgct aaaaaagata tgaatagcac atcaatgatg atgaaaaaag ataagatgac
    18781 aatgcctatg aagaaagaaa tgacttcatc taaaataaat accggtatga tgatgtctaa
    18841 caacaagatg tcagctaata tgcaaatgtc atcacaagcc aaatcaaatg ataaagctgg
    18901 taaaaaaatg tctatgatgt ctaaaaattt acctaataca ggtgaaacaa agcaacaaaa
    18961 tgtaggtgtg ctaggtatgc ttagtctagc ctttgcgaca ggtctaactg cgctaggcct
    19021 taaaaaatct aaacaaagat agggatagta tttaattcag aacaccaaat ttgtttaaaa
    19081 ttattgaggg ttatcgtggt aaaatagtgt tatactagag gtatagcact attttgactc
    19141 attaggagag tagaatgaca caaaaattat tattagttga cgatgaattt gaaattattg
    19201 atattaatag acgttatcta gagcaagcag gatatgaagt gtcagtagct gcggatggca
    19261 tagaagcact caaagaggta gatgagaatc gttttgacct aattattagt gatattatga
    19321 tgcctaaaat ggatggctat gattttatta gtgaagttct agttcgagaa cccaaccaac
    19381 cttttctctt tataaccgct aaagtatctg aaccagataa aatttactct ttgagtatgg
    19441 gtgcagatga ctttatttcc aagcctttca gtcctaggga attagtttta cgtgttaaaa
    19501 atattctaag acgtatctat gggaatcatc aacaatctga agtattgacg attggtgatt
    19561 tggttatcga ccaaaaacaa cgtttggtaa tggtagactg caatacaatt tccttaacca
    19621 ataaatcatt tgacttgctt tggatattag caaatcattt gaatcgtgtt ttttcaaaaa
    19681 cagagcttta tgaacgtgtc tggggcgaag aatttttaga tgacactaat actttgaatg
    19741 ttcatatcca tgccttacgc aatgaccttg ctaaatttag tacagacaat accccaacta
    19801 tcaaaacagt ttggggatta gggtataaat tagaggaata aaatgaaatt aaaatattat
    19861 attgttattg gttaccttat ttctatgtta ataactgtag caggtgtttt ctttgggctt
    19921 aatcatatgc tgattgagac tcgaggtgtt tactacatcc tttcagttac aattattgcc
    19981 tgtatcgtag gtggtatagt taatttgttc ttattatcaa gtgtatttac atctttgaaa
    20041 aagttaaaac aaaaaatgaa ggatatttct caaagacgtt ttgatacaaa agcacaaatt
    20101 tgttcaccac aagaattcaa ggatttagag acagccttta accaaatgtc ttcagagtta
    20161 gagtcaacgt ttaaatctct aaacgagagt gaacgcgaaa aaacaatgat gattgcacaa
    20221 ctttcacatg atatcaagac tccaattacc tctatacaat caacagtaga aggtatttta
    20281 gatggtatta tatctgaaga agaagttaac tattatctca ataccatttc tcgtca
//
LOCUS       DATRTN010000024        20109 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.024, whole genome shotgun sequence.
ACCESSION   DATRTN010000024 DATRTN010000000
VERSION     DATRTN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20109
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.024"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(569..1207)
                     /locus_tag="VBE22_000934"
     CDS             complement(569..1207)
                     /locus_tag="VBE22_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO7202404.1"
                     /translation="MIKSKILLQLMQGKIVAVIRGNSQEEAFQAAQACIKGGISAIEI
                     AYTNSKASQVIEQLVTQYTNQEQVVVGAGTVLDSETARMAILAGAKFIVSPAFNLQTA
                     KLCNRYAIPYLPGCMTLSEVTTALEAGCEIIKIFPGGTLGTSFISSLKAPLPQVQIMV
                     TGGVNLTNAKDWFLSGVTAIGIGGEFNKLAALGEFDKITEMAKQYKIQLNLN"
     gene            complement(1219..2226)
                     /locus_tag="VBE22_000935"
     CDS             complement(1219..2226)
                     /locus_tag="VBE22_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO7202405.1"
                     /translation="MSKILFFGEPLIRITPKENDYFADSISTKLFYGGSEVNTARALQ
                     GFGQDTKLLSALPNNPIGNSFLQFLKAQGIDTHSIQWVGERVGLYFLEDSFACRKGEV
                     VYDRDHSSLHDFRINQIDFDQLFEGVSLFHFSGITLSLDESIQEITLLLLKEAKKREI
                     TISLDLNFRSKLISPKNAKILFSQFATFADICFGIEPLMVDSQDTTFFNRDEATIEDV
                     KERMISLINHFDFQVIFHTKRLQDEWGRNHYQAYIANRKQEFVTSKEITTAVNQRIGS
                     GDAFVAGALYQLLQHSDSKTVIDFAVASASLKCALEGDNMFETVTAVNKVLNQSKDII
                     R"
     gene            complement(2252..2890)
                     /gene="dhuI"
                     /locus_tag="VBE22_000936"
     CDS             complement(2252..2890)
                     /gene="dhuI"
                     /locus_tag="VBE22_000936"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase DhuI"
                     /protein_id="HEO7202406.1"
                     /translation="MKIALINENSQASKNTIIYKELKAVSDEKGFEVFNYGMYGKEEE
                     SQLTYVQNGLLTAILLNSGAADFVITGCGTGIGAMLACNSFPGVVCGFAADPVDAYLF
                     SQVNGGNALSLPFAKGFGWGAELNLRYLFERLFEDEKGGGYPKERAVPEQRNARILSE
                     IKQITYRDLLSVLKEIDQDFLKETISGEHFQEYFFANCQNQNIADYLKSVLD"
     gene            complement(2907..3719)
                     /gene="dhuD"
                     /locus_tag="VBE22_000937"
     CDS             complement(2907..3719)
                     /gene="dhuD"
                     /locus_tag="VBE22_000937"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric 2-dehydro-3-deoxy-D-gluconate
                     5-dehydrogenase DhuD"
                     /protein_id="HEO7202407.1"
                     /translation="MTEQFLKDNFSLEGKVALITGASYGIGFSIATAFARAGATIVFN
                     DIKQELVDKGISAYKKLGIKAHGYVCDVTDEDGINEMVDKISQDVGVIDILVNNAGII
                     KRTPMLEMSAADFRQVIDIDLNAPFIVSKAVLPGMIQKGHGKIINICSMMSELGRETV
                     AAYAAAKGGLKMLTKNIASEYGSANIQCNGIGPGYIATPQTAPLRERQDDGSRHPFDQ
                     FIIAKTPAARWGEAEDLGAPAIFLASDASNFINGHILYVDGGILAYIGKQPQ"
     gene            3997..4431
                     /locus_tag="VBE22_000938"
     CDS             3997..4431
                     /locus_tag="VBE22_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7202408.1"
                     /translation="MIKIIIVAHGNFPDGILSSLELIAGHQEYVVGINFIAGMSSNDV
                     RVALQREVIDFKEILVLTDLLGGTPFNVSSALSVEYTDKKIKVLSGLNLSMLMEAVLS
                     RTMFEHVDDLVDKVITSSHEGIVDFSTCLATQTAEATFEGGI"
     gene            4434..5630
                     /locus_tag="VBE22_000939"
     CDS             4434..5630
                     /locus_tag="VBE22_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEO7202409.1"
                     /translation="MMKIKPVKVESIENPKRFLNSRLLTKIEVEEAIEKALKQLYINI
                     DYFGEEYPTPATFNNIYKVMDNTEWTNGFWTGCLWLAYEYNQDKKLKNIAHKNVLSFL
                     NRINNRIALDHHDLGFLYTPSCTAEYRINGDVKALEATIKAADKLMERYQEKGGFIQA
                     WGELGYKEHYRLIIDCLLNIQLLFFAYEQTGDEKYRQVAVNHFYASANNVVRDDSSAF
                     HTFYFDPETGEPLKGVTRQGYSDESSWARGQAWGIYGIPLSYRKMKDYQQIILFKGMT
                     NYFLNRLPEDKVSYWDLIFTDGSGQPRDTSATATAVCGIHEMLKYLPEVDPDKETYKY
                     AMHTMLRSLIEQYSNNELIAGRPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYK
                     DWELYW"
     gene            5685..6179
                     /locus_tag="VBE22_000940"
     CDS             5685..6179
                     /locus_tag="VBE22_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEO7202410.1"
                     /translation="MAAGPNIVMTRVDERLIHGQGQLWVKFLSCNTVIVANDDVSKDH
                     LQQTLMKTVVPESIALRFFDIQKVIDIIHKANPAQTIFIIVKDLKDVYRLVAGGVPIK
                     EINIGNIHNGEGKEQVSRSIFLGMKDKEIIRKLNQEYHIAFNTKTTPTGNDGAVEVNI
                     LDYI"
     gene            6215..7081
                     /locus_tag="VBE22_000941"
     CDS             6215..7081
                     /locus_tag="VBE22_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO7202411.1"
                     /translation="MDISILQAVLIGLWTAFCFSGMLLGLYTNRCIVLSLGVGVILGD
                     IQTALAVGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITTAGTKGKITPEAALA
                     LSTPIAVGIQFLQTATYTAFAGAPETAKKALQAGNFRGFKIAANGTIWAFAGLGFGLG
                     VLGALSTQTLTDLFALIPPVLLNGLTLAGKMLPAIGFAMILSVMAKKELIPYILLGYV
                     LAVYFGLPVLTPTANGDGVLTSVATNSVLGVPTIGVAIIATIFALLDIFRKPAAPTKE
                     TKTEGDNQDDWI"
     gene            7068..7883
                     /locus_tag="VBE22_000942"
     CDS             7068..7883
                     /locus_tag="VBE22_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO7202412.1"
                     /translation="MTGSKKLAKSDYTKTALRAFYLQNGFNYSNYQGLGYANVIYPAL
                     KKYYGDDKKALAGALEENVEFYNTNPHFLPFVTSLHLAMLDNERPEEEIRGIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLATDGLVMGPILFFVAMNTILTGIKLVTGMYGYR
                     LGTSFIDKLSEQMSVISRAANIVGVTVISSLAATQVKLTIPYTFAPEKVTSTTQKIVT
                     VQGMLDKIAPALLPALYTFLMFYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            7963..9867
                     /locus_tag="VBE22_000943"
     CDS             7963..9867
                     /locus_tag="VBE22_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEO7202413.1"
                     /translation="MKDRLYNKFKDFDREFCQKYIKTYQSNAYQEMKASVNLMMRNTF
                     VFNDNWDMEPCSKAYCLDPLEWDKPVTDDPEWLYMLNRQTYLFKFLVVYIVEGDKSYL
                     RQMKYFMYHWIDCQFTLKPEGAVSRTIDTGIRCMSWLKVLIFLDYFGLITETKKIKLL
                     TSLREQITYMRDYYREKDSLSNWGILQTTAILACLYYYEDELNLPEIQSFAEEELLLQ
                     IKLQILDDGSQYEQSIMYHVEVLKSLMELVILAPKYYLPLEETIEKMVTYLIAMTGPD
                     YCQLAIGDSDVTDTRDILTLATLVLKSSKTKSFSFDNVNLETLLLFGKPSIYLFEEIP
                     RATIGESAYLFPDSGHVCLRDDRRYIFFKNGPFGSAHTHSDNNSVCLYDKKKPIFIDA
                     GRYTYKEEQLRYDFKRSTSHSTCTLDGQPLEMIKDSWTYNSYPKCDYCQLTSKDRYHL
                     VEGQLHVQRASDIYYHKRWLLTLPQAITLVIDKVSCPGEHVLTNQYILDDQVIYENGF
                     VNDLKLVSPTTFNLEDCLISKRYNQLTESHKLVKKIKFVDEVMDYTLIVDRNCQVKCV
                     PLVQTNSHKELSNSIAFDIRSQDFHYLIGVLMDDIIFGDKLYLMQGIKCKGKVIVYDK
                     NNGKMSRLKN"
     gene            9947..10948
                     /locus_tag="VBE22_000944"
     CDS             9947..10948
                     /locus_tag="VBE22_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7202414.1"
                     /translation="MSKKMTINDIAQLSKTSKTTVSFFLNQKFEKMSDETRQRIQEVI
                     DETGYRPSTIARSLNSKKTKLLGVLIGDITNTFSNQIVKGIEHITKQKGYQIIVGNSN
                     YDAKSEEDYIENMLNLGVDGFIIQPTSNFRKYSRILKEKKKPMVFFDSQLYEHKTSWV
                     KANNYDAVYDMTQECLNRGYKKFIMITGDTSLLSTRIERASGFMDALKDNGFGYDTLV
                     IEDDDHSKSDIEDFLKAVVPDKEETLVFAPNCWALPMVFTAMKNLNFDMPRVGLVGFD
                     NIEWTDFSSPKVSTIVQPAYEEGEQVAQILINRIEGDDSVDNQQIVDCQMFWKESTF"
     gene            complement(11019..11633)
                     /locus_tag="VBE22_000945"
     CDS             complement(11019..11633)
                     /locus_tag="VBE22_000945"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO7202415.1"
                     /translation="MSAIDKLVKASHLIDMNDIIREGNPTLRKVAEEVTFPLSEKEEI
                     LGEKMMQFLKHSQDPIMAEKLGLRGGVGLAAPQLDISKRIIAVLVPNVEDAQGNPPKE
                     AYSLQEVMYNPKVVSHSVQDAALSDGEGCLSVDREVPGYVVRHARVTIEYFDKTGEKH
                     RLKLKGYNSIVVQHEIDHIDGIMFYDRINEKNPFAVKEGLLILE"
     gene            complement(11699..12343)
                     /locus_tag="VBE22_000946"
     CDS             complement(11699..12343)
                     /locus_tag="VBE22_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEO7202416.1"
                     /translation="MDIENYCQDYCLETIFPKHYFKDLQVITLTAGQSVCKQGEQLEY
                     LHYIVKGRFKIVRRLFNGKEHILDIKTKPTLIGDIELLTNRQIVSSVIALEDLTVIQL
                     SLKGRKEKLLTDATFLLKLSQELAQAFHDQNIKASTNLGYTVKELLASHILAIEEQGY
                     FQLELSSLADSFGVSYRHLLRVIHDMVKEGLIQKEKPKYFIKNRFALESLNIQP"
     gene            12455..13618
                     /locus_tag="VBE22_000947"
     CDS             12455..13618
                     /locus_tag="VBE22_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7202417.1"
                     /translation="MKIFMEKLSLLSLSLILLSTFSTSPALPQMISYYRDKGLPSPQV
                     ELLFSIPSMAIIFILLITPWLSKKLSEKHMIIFGLLLTALGGGLPVVSQNYLLVFVSR
                     LLLGSGIGFINARAISVISEYYQGKERRKLLGLRGSFEVLGNAGLTALVGLLLTFGWS
                     KSFMIYFLALPILVLYLVFAPKKVVKDTNDKIKTKGQKIPKADLTYIVALAILAGFVI
                     TINTGINLRIPLLVVEFGLGTPAQASLVLSAMMLMGIIAGMSFGQLIAMFHKQLIPIC
                     LVLFSLTLLGVGLPSNLMVLTISAMASGFLYSLMVTAVFSLVADRVEYSLVGSATTLV
                     LVFCNIGGASAAILLSCFDHLLGQINAVFYVYAILSLAVGMIYFFKRLFFKKM"
     gene            complement(13664..14647)
                     /locus_tag="VBE22_000948"
     CDS             complement(13664..14647)
                     /locus_tag="VBE22_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7202418.1"
                     /translation="MIEKYYNWATLGTGVIANELAQALEARGQKLYSVANRTYDKGLE
                     FATKYGIQKVYDHIDQVFEDPEVDIIYISTPHNTHISFLRKALANGKHVLCEKSITLN
                     STELKEAIDLAETNHVVLAEAMTIFHMPIYRQLKTLVDSGKLGPLKMIQMNFGSYKEY
                     DMTNRFFSRDLAGGALLDIGVYALSCIRWFMSEAPHNITSQVTFAPTGVDEQVGILLT
                     NPANEMATVSLSLHAKQPKRATIAYDKGYIELFEYPRGQKAVITYTEDGHQDILEAGK
                     TENALQYEVADMEEAVSGKTNHMYLNYTKDVMDIMTQLRQEWGFTYPEEEK"
     gene            14795..16690
                     /locus_tag="VBE22_000949"
     CDS             14795..16690
                     /locus_tag="VBE22_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEO7202419.1"
                     /translation="MIKYQDDFYQAVNGEWAKTAVIPDDKPRTGGFSDLADDIEALML
                     STTDKWLADENKPSDTILNHFIAFHKMTADYQKREEVGVSPVLPLIEEYKGLQSFSEF
                     ASKVAEYELEGKPNEFPFGVAPDFMNAQLNVLWAEAPGIILPDTTYYSEDNEKGKELL
                     AFWRKSQEDLLPLFGLSEQEIKDILDKVLALDAKLAQYVLSREESSEYVKLYHPYNWE
                     DFTKLAPELPLDAIFQKILGQKPDKVIVPEERFWTEFASDYYSESNWELLKADLILSA
                     ANAYNAYLTDDIRIKSGVYSRALSGTPQAMDKKKAAYYLASGPYNQALGLWYAGEKFS
                     PEAKADVEHKIATMIDVYKSRLEKADWLAQSTREKAIMKLNVITPHIGYPEKLPETYT
                     KKIIDPKLSLVENATNLDKISIAYGWSKWNKPVDRSEWHMPAHMVNAYYDPQQNQIVF
                     PAAILQEPFYALEQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNNWWTDEDFE
                     AFKKLTDKVVEQFDGLESYGAKVNGKLTVSENVADLGGVACALEAAQRESDFSARDFF
                     INFATIWRMKARDEYMQMLASVDVHAPAQWRTNITVTNFEEFHKEFDVKDGDNMWRPV
                     EKRVIIW"
     gene            complement(16740..17693)
                     /locus_tag="VBE22_000950"
     CDS             complement(16740..17693)
                     /locus_tag="VBE22_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEO7202420.1"
                     /translation="MKKLTKGSHIRVLSPSSSIERLGGFEANLLAKEVLEKLGFQVSF
                     SKHYLENDILYSASIASRVEDLHEAFADPSVDAILATIGGFNSNELLPYLDYDLISKN
                     PKIICGYSDSTAFLNAIFAKAKIQTYMGPAYSSFKMKEGQPYQTQAWLTAMTENHYEL
                     WPSEEWSSDPWYDPSKPRQFFPTEWKIYNHGKASGTIIGGNLSTFGLLRGTPYAPKIE
                     RYVLLIEEAEESNFYEFDRNLAAILQAYPHPQAILMGRFPKECGMTPQVFEYILSKHA
                     IFKEIPVIYDMDFAHTQPLLTVTIGAELSVDTTTLSLSIKE"
     gene            17860..18096
                     /locus_tag="VBE22_000951"
     CDS             17860..18096
                     /locus_tag="VBE22_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202421.1"
                     /translation="MSQLSLEEQLNRLKDKYLSSKNQEFSNVHLSKVKKKKKKLIILE
                     KERCRCRLEREDTKKIDDKIEKLKKELIDVMKEE"
     gene            18098..>20109
                     /locus_tag="VBE22_000952"
     CDS             18098..>20109
                     /locus_tag="VBE22_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEO7202422.1"
                     /translation="MEETILIVSFLLFLILSNVINRIFPKLPLPFIQLVFGILSGLVF
                     HKSQVHIDPELFLAFVIAPLNFREGQESDIGSFIKYRAIILYLILPTVFLTAIVVGYV
                     AGHLLPVSLPLAACFALGAALGPTDAVAFISIAKRFQFPKRAENILKLEGLLNDASGL
                     VSFQFALTALVTGYFSLAKASLKLALAIMGGFLIGLLFAFLMRLCLTVLEKFDAADVT
                     GALLLELTLPFVAYFVADLLGFSAIIAVVVAGVMQANRLKKVTLFDAQVDRVTSVIWE
                     TLNFILNGLVFLIFGRELTRIIGPLLTSNAYSNFDLISIVVLVTCTLFLVRFLAVSCF
                     YAWRSFKYHKSFKKYWREIQLLTFSGVKGSVSIATILLLPKHSVIGELGYSLILFTVG
                     AVTLMSFLTGLLVLPKLAPPLQVKDDYLIRLSILTKVLSVLEEDGKSSENQASFYAVI
                     DNYNSRIRHLILEQESSDIKKDLAELQLMMLSIESDGLEAAYRYGNISIKEYRIYQRY
                     LKYLERQVNRSFVSNITYSLTILMRVIRGIVHEFLTFGQGIRNRKKHFKNRKRLLAEV
                     NRQHLTDLYLSNNDLILETLEDMEGYYNATLIDFLQNERIRDAELIKSGLFVERVIAR
                     ITPDILNETLRGYYLERRTISEFERDGLISSKESQILRDNVNELES"
BASE COUNT         6361 a         3258 c         3924 g         6566 t
ORIGIN      
        1 agtcgttata aaaaataaaa tcagtcaaat tagaaaggag gtttagcgtg tctaaaaaac
       61 gactattaat gatatcactc aactatcaaa acttcaaaat aacggtacac tcaaaagctg
      121 agacgcagaa aaaatacttc tgttgaaatc taataaacta taatcagaaa tgtttttaat
      181 tatgtttaaa aaatgtattt gataggagct aagttagtct gtgttgagat aggaaaagcg
      241 ggtaagcctg ataggtcatt gctaagtttt tggatataaa aatgatatca aatctattat
      301 ttgctgaaat gatatgagaa tgatgagctt cactacttat atcaatctgc gggcaagtag
      361 tatatctttg gtgaagacat ggatcagttg tcggaagtag aacggttatg tctacaggaa
      421 gattagctaa aaattatctc ggtctgataa gaaaatcgag ataatgagtc tgattaagat
      481 aaacggtact acagaaaaat ggctttataa aagttctttt cttctgtctc atgattgagg
      541 tgtgggggtg ccttgtcttt ttattgtgtt aattcagatt tagctgtatt ttgtattgtt
      601 tagccatttc tgtgatttta tcaaattccc ccaaagctgc tagtttattg aactctccac
      661 caataccaat ggctgttact ccagatagaa accagtcttt ggcatttgtg agatttaccc
      721 caccagtcac cataatttgt acttgaggta aaggtgcctt tagtgatgag atgaaagatg
      781 ttcctagagt acctcctggg aatattttaa ttatttcgca accggcttca agtgctgttg
      841 ttacctctga taaggtcatg caacctggta aataaggaat agcataacga ttacatagtt
      901 tagcagtctg tagattaaat gcgggagata caataaactt tgctcctgct aaaatagcca
      961 ttcgggcagt ttctgagtct agaacagttc ctgccccaac gacaacttgt tcttggttag
     1021 tatattgtgt gactagttgt tcaatgactt ggctagcttt gctattagta taggcgattt
     1081 cgatagcaga aataccacct tttatgcatg cttgagcagc ttgaaaagct tcttcttggc
     1141 tatttccacg aatgacagca actatttttc cttgcattaa ttgcaacaaa atctttgatt
     1201 ttatcataat tctcccttct aacgaataat gtctttcgat tgatttagta ctttgttaac
     1261 tgcggtaacg gtctcaaaca tattgtcccc ttccaaagca cattttaaag atgcactagc
     1321 gacagcgaaa tcaatgactg tcttgctgtc tgagtgctga agtagttgat atagcgctcc
     1381 cgccacaaaa gcatctccgc ttccaattct ctggtttaca gctgtggtga tttcttttga
     1441 agtgacaaac tcttgtttac gatttgctat gtaagcttgg tagtggtttc gtccccattc
     1501 atcctgtaga cgtttagtat ggaaaatgac ttgaaagtca aagtgattta taagcgaaat
     1561 cattcgttct ttgacgtcct ctattgtagc ttcatcgcga ttgaagaagg tagtgtcctg
     1621 tgagtcaacc ataagaggtt caataccaaa acaaatatcc gcaaaggtag cgaactgaga
     1681 aaaaaggatt ttggcatttt taggagaaat tagtttactg cgaaaattga gatctaagga
     1741 gattgttatc tctctttttt tagcttcttt taaaagaagc agtgttatct cttggataga
     1801 ctcatccaat gatagtgtaa taccactaaa atgaaagaga gaaactcctt caaataattg
     1861 atcgaaatca atttgattta tcctaaagtc atgtaaacta ctatgatcgc ggtcgtagac
     1921 cacctcacct tttctgcaag caaaggaatc ttctaaaaaa tataacccaa cacgttctcc
     1981 aacccactga atagaatgag tatcaatacc ctgtgctttt aaaaattgaa gaaaactatt
     2041 acctatggga ttattgggta aggctgagag gagtttagta tcttgcccaa agccttgtag
     2101 agctcttgcg gtattaactt cagagccacc atagaatagt ttagttgaga tactatctgc
     2161 aaaatagtca ttttcttttg gagtgatgcg aatgagaggt tcaccgaaaa aaagaatctt
     2221 agacataaaa gtcctttcta aaacagacgc tttagtctaa gacagatttc aagtagtctg
     2281 ctatattttg attttggcaa ttagcgaaga aatattcttg gaaatgctcc ccagaaatag
     2341 tttcctttaa gaaatcttga tcaatttctt ttaaaacaga gagtaaatct ctgtaagtaa
     2401 tctgttttat ttcgctaagg atacgtgcat tgcgttgctc aggtacagcc ctctctttag
     2461 ggtatccccc tcctttttca tcttcgaaaa gacgttcaaa taaatacctc agattcaatt
     2521 cagctcccca accaaatcct ttagcaaaag gaagggaaag agcatttcct ccgtttactt
     2581 gagagaataa gtaagcgtcg acaggatcag cagcgaatcc acagactacg ccaggaaaac
     2641 tattacaagc aagcatcgct ccaattcctg tcccacagcc tgtaataaca aaatctgcag
     2701 caccgctatt aagtaaaata gcagtcagta aaccattttg tacataagtt agctggcttt
     2761 cttcttcttt gccatacatt ccgtagttga aaacttcaaa tcctttttca tcagaaaccg
     2821 ctttcaattc tttgtagata atagtatttt tacttgcttg gctattttcg ttgattaatg
     2881 caattttcat ttatattact cctttattat tgaggttgtt taccgatgta agctaaaata
     2941 ccgccatcta catagagtat gtgaccattg ataaagtttg atgcatcact agcaaggaag
     3001 atagctggtg cacctaaatc ttctgcctca ccccagcgag cggcaggtgt tttagcgatg
     3061 ataaattggt cgaatggatg acgtgaacca tcatcttgac gttcacgaag gggagctgtt
     3121 tgtggtgtgg caatatagcc gggcccgata ccgttacatt ggatgttagc agaaccgtat
     3181 tcagaagcga tgttcttggt taacatttta agtccccctt tggcagcagc ataggcagca
     3241 actgtttcgc gtccaagctc actcatcatg gagcaaatat tgatgatttt accatgtcct
     3301 ttttggatca ttccaggtaa taccgctttt gatacgataa agggagcatt caaatcaata
     3361 tcaataactt gtcgaaaatc tgcagcgctc atctctagca taggggtgcg ttttataata
     3421 cctgcgttgt taactagaat atcaattacc ccaacatctt gagaaatttt gtccaccatt
     3481 tcgttgattc catcttcatc tgtaacatca caaacatagc catgggcctt aatgccaagt
     3541 tttttatagg cggagatgcc tttgtcaacg agttcttgct taatatcatt gaagacaata
     3601 gttgctccag ctctagcaaa agcagttgct attgaaaaac caatgccgta agaagcgcct
     3661 gttattaagg cgactttacc ttctagtgaa aagttgtctt ttaagaattg ttcagtcata
     3721 atatgacctc cgtaaaaaaa ttcaggaagt aaacctgttt acatgaatag taacaaagta
     3781 aaccggtttt gtcaactata gagttcttct tctataaaac ttggcaacat atataacaaa
     3841 gatttatcta ggttataatt cttaaagata aaaaagacat cattataaaa aatcaagtaa
     3901 actgctcaaa aaatataaaa aagatgttga cacctaaaaa tgaaagcgct ataatctgat
     3961 tataaaagta aaccggtaaa ctcaagagga gaaaatatga taaaaattat tattgtagca
     4021 cacggcaact ttccagatgg tattttaagc tctttagaac ttattgctgg gcatcaggaa
     4081 tatgttgttg gtattaactt tattgcaggc atgtcttcta atgatgttag agttgctttg
     4141 caaagagagg ttatcgattt caaagaaatt ttagtattga ctgatttgct aggtgggact
     4201 cctttcaatg tttcttccgc cttatcagta gaatatacag ataaaaagat aaaagtttta
     4261 tcgggcttga acctttcaat gttaatggaa gctgttttat ctagaacgat gtttgaacat
     4321 gtagatgatc ttgttgataa agttataaca tcatctcatg aaggaattgt tgatttttct
     4381 acttgccttg ctacacaaac tgcagaagca acttttgagg gaggcattta ataatgatga
     4441 aaataaaacc ggtcaaggtt gagtcaattg aaaatccaaa gcgtttttta aacagtagat
     4501 tattaactaa gattgaagtt gaggaagcga ttgaaaaagc cttgaagcaa ctttatatta
     4561 atattgatta ctttggtgaa gagtatccaa cgcctgcaac attcaataat atttataaag
     4621 ttatggataa cacagaatgg acaaatggtt tttggacagg gtgcttgtgg ttagcttatg
     4681 agtataatca ggataaaaag ttaaaaaaca tagcccacaa aaatgtattg tcatttctaa
     4741 atcgtattaa taatcgtata gcattagatc accacgactt aggatttctt tacacaccat
     4801 cttgtacagc agaatatcgt atcaatggtg atgttaaagc tttagaagcc actataaaag
     4861 ctgcagataa attgatggag cgctatcaag aaaaaggtgg atttattcag gcttggggag
     4921 aactcgggta taaggaacac tatcgcttaa ttatcgattg cttacttaat atccaactct
     4981 tattttttgc ttatgaacag acaggtgatg aaaagtatag acaagttgcg gtgaatcact
     5041 tctacgcttc agctaacaat gtggtgcgtg atgattcttc tgcttttcat actttttatt
     5101 tcgacccaga aactggagaa ccgttaaaag gtgtcacacg acagggttat agtgatgagt
     5161 catcttgggc aagagggcaa gcatggggca tctacggtat tccgcttagt taccggaaaa
     5221 tgaaagatta tcagcagatt atccttttta aaggtatgac aaactatttt ctaaatcgtt
     5281 tacctgaaga caaggtatcc tattgggacc ttatttttac ggatggctcg ggccagccta
     5341 gagatacatc cgcaacagca acggctgtgt gtggaattca tgagatgctt aaatatttac
     5401 cagaagtaga tcctgataaa gagacataca aatatgctat gcatacaatg cttcgtagtc
     5461 tgattgaaca gtatagtaat aatgaactta tagcaggacg tcctcttcta ttgcacggtg
     5521 tgtattcgtg gcattcaggt aaaggagtag atgaaggtaa tatttgggga gattattatt
     5581 acttagaagc cttaataaga ttctataaag actgggaact ttattggtaa gagtatttta
     5641 ctaacaaaat aaacaataaa taatagaaaa cgaggaggtc agaaatggca gcaggaccaa
     5701 atattgtaat gacacgtgtt gatgagcgtc ttattcatgg acaaggacaa ctttgggtga
     5761 aatttttaag ttgtaatact gttattgtgg caaatgatga tgtctccaaa gatcatcttc
     5821 aacaaacgtt aatgaagacg gtggtgccag aatcaatagc attgcgattt tttgatattc
     5881 aaaaagttat tgatattatt cacaaggcca acccagcaca gacgattttt ataattgtta
     5941 aagatttgaa agatgtctat agacttgtag cagggggcgt cccaattaaa gagattaata
     6001 ttggaaatat tcataatgga gaaggtaaag agcaggtaag tcgctctatc ttcttaggga
     6061 tgaaggataa agagataatt agaaaattaa accaagaata tcacattgca tttaatacta
     6121 aaacaactcc aacaggtaac gatggtgctg tggaagttaa tattttagat tatatttagt
     6181 tggacaaact tactatcata taaaggagaa aaatatggat attagtattc ttcaggctgt
     6241 cttaattgga ctgtggacgg ctttctgttt tagtggcatg ttgcttggcc tctatacaaa
     6301 tcgttgtatc gttctctctc taggcgttgg tgttatttta ggagatattc aaacagcttt
     6361 ggcagttgga gcaatatctg aactggctta catgggattt ggtgtgggag cgggagggac
     6421 agttcctcca aacccaatcg gtcctgggat ttttggaact ttgatggcta ttacaacagc
     6481 tggaactaaa ggaaaaatca caccggaagc tgctcttgca ttatcaaccc caattgcagt
     6541 tggtattcaa tttcttcaaa cagcaactta tactgctttt gcaggggcgc ctgaaacagc
     6601 taaaaaagct ttgcaagcag gtaatttccg aggatttaaa attgcagcaa atggtacaat
     6661 ttgggcattc gctggattgg gatttggttt aggagtactg ggtgctcttt caacgcaaac
     6721 tttgacagat ttatttgctc tcattccccc tgttttgctt aatggtctaa ccttagctgg
     6781 gaaaatgctt cctgcaattg gttttgcgat gattctctct gtaatggcta aaaaagaact
     6841 cattccttat atcctacttg gatatgttct agctgtttat tttggtttgc cagttctaac
     6901 tccaacagca aatggagacg gagttttaac atcagtggct actaacagtg tgcttggggt
     6961 tccaactatc ggggtagcta taattgcaac aatatttgct ttgcttgata ttttcagaaa
     7021 accagctgca cctactaaag aaactaagac agaaggagac aaccaagatg actggatcta
     7081 aaaaattagc aaaaagtgat tacactaaaa cagctttacg tgccttttat ttacaaaatg
     7141 gctttaatta cagtaactat caaggtcttg gttatgctaa tgtcatatat ccagctctca
     7201 aaaaatatta tggagatgac aaaaaagctt tagcgggagc tctagaagaa aatgtggagt
     7261 tttacaatac aaaccctcat ttcttacctt ttgtaactag cttacattta gcaatgcttg
     7321 ataatgagcg gccagaagaa gaaattcgcg gtattaagat ggcgcttatg gggcctcttg
     7381 caggtatcgg tgattcttta tcacaatttt gcttagcacc cttgttctca acgattgcag
     7441 cctctttagc gacagatggt ttggtgatgg gaccgattct gttctttgtg gctatgaata
     7501 ctattttaac gggaattaaa ttagttactg gcatgtatgg ttatcgtctt gggaccagtt
     7561 ttattgataa attgagtgaa caaatgtcag ttatctcacg ggcagctaat atcgttggtg
     7621 taacggtcat ctcaagttta gctgcaacac aagttaagct tacaatccct tacacttttg
     7681 ctccagaaaa agtaactagt acaacgcaaa agattgttac tgtccaaggt atgcttgata
     7741 aaattgcacc agctctactt ccagcactct acactttcct tatgttttac cttattaaaa
     7801 ataaaaaatg gacaacttat aagttagtta tccttacagt tattattggt atccttggta
     7861 gctggttagg tattttagct taataaaatt ttgataaata aggattatga catatcaatg
     7921 acatagtcct ttttatatcg gacgaggacg ttttgtaaaa aaatgaaaga tcgcttatat
     7981 aataaattca aggattttga tagggaattt tgtcaaaagt atattaaaac ataccaatct
     8041 aatgcctatc aagaaatgaa agcctctgtt aatttgatga tgaggaacac ttttgtattt
     8101 aatgataatt gggatatgga accttgttct aaagcatatt gtcttgatcc tttggagtgg
     8161 gataagccag taacagatga tccagaatgg ttgtatatgc taaatcgtca aacttatctt
     8221 tttaaattcc tggtggtcta cattgttgaa ggcgataagt cttaccttag acaaatgaaa
     8281 tattttatgt accattggat tgattgtcaa tttacactaa agccagaagg ggcggtaagt
     8341 cgtactattg acactgggat acgttgcatg tcatggctca aagttttgat ttttttagat
     8401 tattttggat taataacaga aactaaaaaa attaaactat taactagttt acgggagcag
     8461 ataacttata tgagggacta ttatcgtgaa aaagatagtc taagtaactg gggaatttta
     8521 caaacaacag caatattggc gtgtttatac tattatgaag atgaattaaa tctacctgaa
     8581 attcagagtt ttgctgaaga agaattatta cttcaaatca agcttcaaat tttagatgac
     8641 gggagccagt atgaacaatc aattatgtat catgtagaag tcttgaagtc cttaatggaa
     8701 ctagttatcc ttgcgcctaa atattatcta ccattagaag aaactattga aaaaatggtt
     8761 acctatctaa ttgctatgac tggtccggat tattgtcaac tagctatagg ggacagtgat
     8821 gtgaccgata ctcgtgatat tctaactttg gcaacacttg tgttgaaatc ttctaaaaca
     8881 aaatcatttt cttttgataa tgttaattta gaaactttac ttttgtttgg gaagccatca
     8941 atttatcttt ttgaagaaat accgcgtgcg acaataggag agtctgctta tctttttcca
     9001 gattctggtc atgtgtgttt acgtgatgat aggcgttata tattttttaa gaacggtcca
     9061 tttggtagcg ctcatactca tagtgataat aatagtgttt gtctctatga taaaaagaaa
     9121 cctattttca ttgatgcagg aagatatact tacaaagaag aacaactaag gtatgatttt
     9181 aaacgttcga ctagtcattc aacatgtacc cttgatgggc aacccttaga aatgatcaag
     9241 gactcttgga catacaattc ttatccaaaa tgtgactatt gtcagttgac ttcaaaggat
     9301 aggtaccatt tagtcgaagg acaactacat gtccaaagag cttctgatat ctattaccat
     9361 aagcgatggt tgttaacttt accgcaggcc attaccttag ttattgataa ggtgagttgt
     9421 ccaggagagc atgtcttaac aaatcaatat attttagatg atcaggtcat ttatgaaaat
     9481 gggtttgtta atgacttgaa attagtaagt cctacgacct ttaatctaga agattgcctt
     9541 atttctaagc ggtataatca attgacagaa agtcataaat tagttaagaa aataaaattt
     9601 gttgatgagg tgatggatta taccttgata gttgatcgga actgtcaggt gaaatgtgtt
     9661 cctttagtgc aaacgaacag tcataaggaa ctaagtaata gcattgcatt tgatattagg
     9721 tctcaagact ttcattattt aattggagtg cttatggatg atattatctt tggggataaa
     9781 ctctatttga tgcaagggat aaaatgcaaa ggaaaagtca ttgtatatga taaaaataat
     9841 ggcaaaatga gtcgtttaaa aaattaagag ggctattatt atgctatact agtactatat
     9901 taaccataaa aaaataaaat tagtaaaatt agaaaggagg tttgttatgt ctaaaaaaat
     9961 gaccattaat gacatcgctc aattatcaaa aacttccaaa acaacggttt cttttttctt
    10021 gaatcaaaaa tttgaaaaaa tgtcggatga gacgaggcaa cgtattcaag aggtgattga
    10081 tgaaacaggt tatagaccaa gtacgatagc aagaagtcta aattctaaaa aaacaaaact
    10141 actaggtgtc ttaattggag atataacaaa cacattttca aatcaaattg taaaaggaat
    10201 tgagcacatc accaaacaaa aaggctatca aattattgtg ggtaatagta attacgatgc
    10261 caaaagtgaa gaagattata ttgaaaatat gttaaattta ggtgttgatg gtttcattat
    10321 tcagcctaca tcaaattttc gaaagtattc tcgtatttta aaagaaaaaa agaaaccaat
    10381 ggtatttttt gatagtcaac tttatgaaca taagacgagt tgggttaagg ctaataacta
    10441 tgatgctgtt tatgatatga cacaggagtg tctaaatcga gggtataaaa agtttattat
    10501 gattactggt gatacgagct tgcttagtac acgtattgag agagcttcgg gattcatgga
    10561 tgctttaaag gacaatggtt tcggttatga cactttggtg attgaagatg atgatcattc
    10621 aaaaagtgat atagaagact ttctaaaagc ggtagttcca gataaggagg agacattagt
    10681 atttgcacca aattgttggg ctttaccaat ggtttttact gccatgaaga atctcaattt
    10741 tgatatgcca cgagttggtt tagttgggtt tgacaatatt gaatggacag acttttcatc
    10801 tcctaaagtg tcaacaattg tccagccggc ttatgaagaa ggtgaacaag tagcacagat
    10861 tttgattaat cgtattgaag gagatgactc cgtcgataac caacagattg tagattgcca
    10921 gatgttttgg aaagagtcga ctttttaaaa aagatttctt attttatttg ctattaaaaa
    10981 ataaaaccta gagaaaatta tctctaggtt atttgtcctt attctaaaat aagtaagcct
    11041 tctttgacag cgaaaggatt tttctcattg atacggtcgt aaaacataat accatcaatg
    11101 tggtcaattt catgttgaac aacaatagag ttgtaacctt ttaatttgag tcgatgtttt
    11161 tcaccagttt tgtcgaaata ttcaatagtt acacgcgcgt ggcggacgac atagccaggt
    11221 acttcacgat ctactgatag gcatccctcc ccgtcagata aagcagcatc ttgaacagaa
    11281 tgcgatacaa ctttagggtt gtacattact tcttggaggc tataagcttc tttaggtgga
    11341 tttccttgag catcctcaac gtttggaaca aggacggcaa tgatgcgttt tgagatgtct
    11401 aattgtggtg cggctaaacc aacaccacca cgtaagccta gcttctcagc cataattgga
    11461 tcttgagagt gttttaaaaa ttgcatcatt ttttctccga gaatttcttc tttctcagaa
    11521 agtgggaatg tgacttcttc agccactttg cgaagggttg gattgccttc acggataatg
    11581 tcattcatgt caattaaatg actagctttt actaatttat caatagcaga catattttct
    11641 cctaactgta ttctctgatt tagtttagca taaaataatt aaatgataaa gctacgcttc
    11701 atggctgaat atttaatgat tctagtgcga aacgattttt gataaagtat tttggttttt
    11761 ctttttggat aaggccttct ttgaccatat cgtggataac tcttaataaa tgacgataac
    11821 tgacgccaaa cgaatcagct aaactactta actcaagttg aaagtaacct tgttcttcaa
    11881 ttgctaaaat atgacttgca agtaactctt tgacggtata ccctagatta gtagacgctt
    11941 tgatgttttg atcatgaaaa gcctgagcta gctcttgact aagttttagg aggaaagttg
    12001 cgtctgttaa cagtttctct ttccgtcctt taagtgataa ttgaatgact gtaagatcct
    12061 ctaaggcaat aacagatgat acaatttgtc tattagtcaa aagttcaata tcaccaatca
    12121 gtgttggttt tgttttaata tctaaaatat gttctttgcc gttaaagagt cgacgtacaa
    12181 ttttaaaacg tcccttgacg atgtagtgca agtattctaa ttgttctccc tgtttacaga
    12241 cggattggcc ggcagttagg gtgattactt gtaaatcctt gaaataatgt tttggaaaga
    12301 tagtctccaa acagtagtct tggcagtagt tttctatatc catgtattga ctccctattt
    12361 gagatagttt taggacatat gtcatatatt tctagtttaa tattgattat actttattta
    12421 tctaaaaaaa gacatcagaa agaggtattg tcacttgaaa atctttatgg agaagctaag
    12481 tttactctct ttatcactta tcttgctatc aactttttcg acatcaccag ccctaccaca
    12541 aatgatatct tattatagag ataagggctt accatcaccc caagttgaat tattgttttc
    12601 aattccttcg atggctatta tcttcatctt gcttatcaca ccttggttaa gtaaaaaact
    12661 ttctgagaaa catatgatta tctttgggct attgctaaca gcattagggg gaggattgcc
    12721 ggttgtcagt cagaactacc ttttagtatt tgtatcgcgt cttttattgg ggagtggtat
    12781 tggttttatt aatgctaggg ccattagtgt gattagtgaa tattatcaag gcaaagaaag
    12841 aagaaaactc ttaggattaa gggggtcctt tgaagtttta ggaaatgctg gtttgacagc
    12901 tttagttggg ctgttgttga cttttggttg gtccaaatca tttatgattt attttttagc
    12961 tttaccaatc ttagttctat atcttgtttt tgcgcctaag aaagtggtga aagatacaaa
    13021 tgacaagatt aaaactaaag gtcaaaaaat ccctaaagct gatttaactt atattgtcgc
    13081 cttagctatt ttagcaggat ttgtcataac tataaatact gggattaatc ttaggatacc
    13141 tctgctagta gtagaatttg gtttgggtac gccagctcaa gctagtcttg ttcttagtgc
    13201 gatgatgctt atgggaataa tagcaggtat gagttttggt cagttgatag ctatgtttca
    13261 taaacaatta attccaatct gcctcgtctt attttcttta acattgcttg gagttggatt
    13321 gccttctaat ctaatggtac taacaattag tgcgatggca tcaggtttcc tttacagcct
    13381 gatggtcact gctgtgttta gcctagtagc agaccgtgtt gaatattcgt tggttggttc
    13441 tgcaacgaca ttagttttag tattttgtaa tattggtgga gctagtgcgg ctattcttct
    13501 gtcttgtttt gatcatttac taggacaaat aaatgcagtt ttctatgttt atgctatttt
    13561 aagtttagca gttggtatga tttatttctt taaacgccta ttttttaaga aaatgtagtg
    13621 tatattaact aaaaagggct tgacaatcat tgtcaagctc tcttcatttt tcttcttctg
    13681 ggtaggtaaa tccccattct tgacgtagct gtgtcatgat atccataacg tctttggtat
    13741 agtttaagta catgtggtta gtttttcctg aaacggcttc ttccatatca gctacctcat
    13801 attggagggc attttcagtt ttgccagctt cgagaatatc ttgatgccca tcctcagtat
    13861 aagtaataac tgccttttgt cctcgcggat attcaaaaag ttcaatgtag cctttatcgt
    13921 aagcgatagt tgctcgttta ggttgttttg catgtaaact aaggctgaca gtcgccatct
    13981 catttgcagg attggttagt aggataccaa cttgttcatc aactcctgtt ggtgcaaatg
    14041 taacttgaga ggtaatgttg tgaggtgctt ctgacataaa ccagcgaata caagaaagtg
    14101 cataaacacc aatgtccagc aaagcaccgc ctgctaggtc acgactgaaa aaacggttag
    14161 tcatatcata ttctttataa cttccgaaat tcatttgaat catttttaac ggtcctaatt
    14221 ttccactatc aactaatgtt tttaattggc gataaattgg catatgaaaa atagtcatgg
    14281 cttcagctaa gacaacatgg ttagtttcgg ctaaatctat ggcttcttta agttcagtgc
    14341 tgtttaaagt aatagatttt tcgcaaagaa cgtgcttacc atttgctaaa gcctttcgta
    14401 aaaatgagat gtgagtattg tggggagtag agatataaat gatatccact tcagggtctt
    14461 caaatacttg atctatgtga tcataaactt tttggatacc atatttggta gcaaattcaa
    14521 gtcctttgtc gtaagttcta ttagctacag aatataattt ttgtccacgt gcttctagtg
    14581 cttgggctaa ttcgttggca ataacgccgg ttccaagcgt tgcccaatta taatattttt
    14641 caatcataac aacctccttg ttggtttata gtatagcaaa aataacaaaa tgatatttag
    14701 agaaagcacc aatatgagag tatttttaat agtcaaactc tgaattttct gttagactgt
    14761 aagagattac atatgagata ttaaggagaa aataatgata aaatatcaag atgattttta
    14821 ccaagcggtt aatggggagt gggctaagac agctgttatt cctgatgaca aaccaaggac
    14881 aggtggtttt tctgatcttg ctgatgacat tgaagcatta atgctgtcaa cgacagataa
    14941 gtggctggct gatgaaaata aaccaagtga tacgatttta aatcatttta ttgcttttca
    15001 taaaatgaca gctgattatc aaaaacgtga agaggtagga gtatcaccag tattaccatt
    15061 aattgaagaa tataaaggcc tacaatcttt ctctgagttt gcatcaaaag ttgcagaata
    15121 tgaactagag ggtaaaccaa atgaatttcc atttggtgtg gctcctgact ttatgaatgc
    15181 tcaattgaat gtactttggg ctgaagcgcc tgggattatt ttaccagata caacttatta
    15241 tagtgaagat aatgaaaaag gaaaggaact ccttgctttt tggcgtaaaa gtcaagaaga
    15301 cctactgcct ttgtttggcc tttcagaaca ggaaattaaa gatatcttgg ataaggtttt
    15361 agcattggat gcaaaattgg cacaatatgt cttatctcgc gaagagagtt cggaatatgt
    15421 taaactttac catccttata actgggaaga tttcacaaaa ttagcacctg aacttccatt
    15481 agatgctatt ttccaaaaga tattaggaca aaaaccagat aaagttattg ttcctgaaga
    15541 acgtttctgg acagaatttg catctgacta ttactcagaa tctaattggg aattattaaa
    15601 ggcagatctt attttatcag ctgcgaatgc ctataatgct tacttaactg atgatattcg
    15661 tatcaaatcg ggagtttaca gtcgagcttt atcaggcaca ccacaagcaa tggataagaa
    15721 aaaagcagct tactaccttg cttcaggacc ttacaatcaa gctctaggtt tgtggtatgc
    15781 aggagaaaaa ttctctccag aagctaaagc tgatgttgag cataagattg caacgatgat
    15841 tgatgtctat aagtcacgct tagaaaaggc ggattggctt gcacagtcaa cgcgagaaaa
    15901 agctattatg aaacttaatg ttatcacacc acatattgga taccctgaga aactaccaga
    15961 gacttatact aaaaaaatca ttgatcctaa actatcgctt gtcgaaaatg ctactaattt
    16021 agacaaaatt tcaatagcct atggttggag taaatggaat aaaccagttg atagaagtga
    16081 atggcatatg cctgcccaca tggtcaatgc gtattatgat ccacaacaaa accaaattgt
    16141 cttcccagca gccattttac aagaaccatt ttatgcttta gagcaaagtt catcagcaaa
    16201 ttatggggga attggtgcag tgattgcaca tgaaatatca cacgcttttg acactaatgg
    16261 agcttcattt gatgaacatg gaagtttgaa taattggtgg actgatgaag attttgaagc
    16321 tttcaaaaaa cttacagata aagtagttga gcagtttgat ggactggaat cttatggcgc
    16381 aaaagtcaat ggtaaactaa cagtatcaga aaatgttgca gatttaggtg gagttgcttg
    16441 tgctttagaa gctgcgcagc gtgagtcaga tttttcagcg agggatttct ttattaactt
    16501 tgcgacaatt tggcgtatga aggcacgtga tgagtatatg caaatgttag ctagtgtaga
    16561 tgttcacgcc ccagcacaat ggcgaactaa tattacagta acgaattttg aagaattcca
    16621 taaagagttt gatgttaaag atggtgataa catgtggcgt ccagttgaaa aacgtgttat
    16681 tatttggtaa ctctaaatga taaactcccg ttactcagat gagtagcagg agtatttttt
    16741 tattctttta tagatagaga taatgtagta gtgtcaacgc ttagctcagc tccaatagtc
    16801 actgtcaaaa gaggttgggt gtgcgcaaaa tccatatcat aaataacagg aatttccttg
    16861 aaaatagcat gttttgaaag gatatattca aagacttgtg gagtcatgcc gcattcttta
    16921 ggaaagcgcc ccataagaat agcttgtgga tgaggataag cttgtaaaat tgcagcaagg
    16981 ttgcggtcaa attcataaaa attactttcc tcagcctctt caatcaagag gacatagcgt
    17041 tcaattttcg gtgcataagg agtacctcga agtaggccaa acgtagagag attacctccg
    17101 ataatcgtac cggaagcttt tccgtgatta taaattttcc attctgttgg gaaaaattgg
    17161 cgtggtttgc tgggatcata ccagggatca ctagaccatt cttcactagg ccaaagttca
    17221 tagtggtttt ctgtcatggc agtaagccaa gcttgtgttt ggtaaggttg accttccttc
    17281 attttgaaag aagaataagc agggcccatg taagtttgga tttttgcttt ggcaaaaata
    17341 gcattaagga aggccgttga atctgagtaa ccacaaatta ttttgggatt cttagagatt
    17401 aaatcataat ctaaatatgg taaaagctcg ttggaattga atccgccaat ggttgctaat
    17461 atggcatcaa cagaagggtc ggcaaatgct tcatgtaaat cttctacacg gctagcaatc
    17521 gaagcagaat agaggatatc attttctaga tagtgcttag agaatgacac ttgaaatccc
    17581 agcttttcta gtacttcttt ggctaatagg ttagcctcaa aaccacccag acgctcaatg
    17641 gaagaagagg gactaagaac acgaatgtgg ctgccttttg ttaatttttt cattggttaa
    17701 cctcctattt aaattgttgt tagtctacca catatggaga taatgagcaa cgatattctt
    17761 aaaatctatc aatttaagaa atgcgtgtta tactagagat agccgatgaa agaaaaaata
    17821 cttattatta ataatttggt cattaaatag gaggtcatga tgtctcaatt atctttggaa
    17881 gaacaattga atcgactaaa agataaatac ctttcttcta aaaatcaaga attttcaaat
    17941 gttcacttaa gtaaagtaaa gaagaaaaag aagaaattaa taatacttga aaaagaaaga
    18001 tgtcgctgtc gattggagag agaagatact aaaaaaatcg atgataaaat tgagaaattg
    18061 aagaaagaat tgatagatgt tatgaaggag gaataatatg gaagaaacta ttctaatagt
    18121 cagctttctc ctgtttttga ttttatctaa tgttatcaat aggatatttc ctaaattacc
    18181 attaccattt atccagttgg tctttgggat tctctctggc ttagtttttc ataagtcaca
    18241 agtccatatc gatcctgagt tattccttgc ctttgttatt gctcctttga actttaggga
    18301 aggtcaagaa agtgatattg gtagttttat aaagtatcgg gctattattc tttacttaat
    18361 cttaccaacg gtatttttaa cagctattgt cgttggttat gtagcagggc accttttgcc
    18421 agttagttta ccacttgcgg catgctttgc gcttggagca gccttaggcc caacagatgc
    18481 cgttgccttt atatcgattg ctaagaggtt tcaatttcct aaacgggcag aaaatatcct
    18541 caaattggaa ggtcttttaa atgacgctag tggtctagtt tccttccagt ttgctttaac
    18601 agcccttgta acaggttatt tctccttagc aaaagctagt ctcaagttag cattggccat
    18661 aatgggtggt tttttaattg gcttgctttt tgcttttcta atgaggttat gtctcactgt
    18721 cctagagaaa tttgatgctg ctgatgttac aggggctcta cttttggaat taaccttacc
    18781 ttttgttgct tatttcgttg ctgatttgtt gggattttca gctattattg cggtggtagt
    18841 ggcaggagta atgcaagcaa atcgtttgaa aaaagtgact ctctttgatg ctcaagtaga
    18901 tcgcgttacc tctgttattt gggaaacctt aaattttatt ttaaatggtt tagttttctt
    18961 aatatttgga agagaactga cacgtatcat tggtcctctt ttaacaagta acgcttatag
    19021 taattttgat cttattagta ttgttgtttt agttacttgc acactttttc tagttcgctt
    19081 tctagcagtt tcttgctttt atgcttggag atctttcaaa taccataaat catttaaaaa
    19141 gtattggaga gagattcaac tgttgacttt ttcaggagtt aaaggaagtg tttccatagc
    19201 aacaatactc ttattaccca agcactcagt cataggagag ttggggtata gtttgattct
    19261 tttcactgtt ggtgcagtaa cgctaatgag ttttttaacg ggacttttag tattaccaaa
    19321 gttagcgcct cccttacagg tgaaagatga ttatttaata cgtctatcga ttttaacaaa
    19381 agtattatct gtcttagaag aagatggtaa gagttctgaa aatcaagctt cattttatgc
    19441 agttatagat aattataata gccgtattcg tcatctcatt ttagaacaag aatctagtga
    19501 cattaagaag gacttagctg aattgcaact gatgatgctt agtatagaaa gcgatggact
    19561 tgaagctgct tatcgttatg gcaatatttc tatcaaagaa tatcgtattt accaacgtta
    19621 tttaaaatac ttagaacgcc aagttaatcg tagctttgtg tcaaatatca cttatagttt
    19681 aacgattctt atgcgtgtta ttcgtggtat tgttcatgag tttttaacat ttggacaagg
    19741 aatacgaaat cggaaaaaac atttcaaaaa caggaaacgg ttgttagcag aagtcaatcg
    19801 tcagcactta acagatttgt acttatcaaa taatgacctc attttagaaa cgctagaaga
    19861 tatggaaggt tattataatg caactttgat agatttctta caaaatgaac gcattcgaga
    19921 cgcagaatta attaaatcag gtttatttgt agaacgtgtc attgcacgca taacgccaga
    19981 tattttaaat gaaaccctaa gaggatatta cttggaacgc agaacaatct ctgaatttga
    20041 aagggacggt ttaattagtt ctaaagagtc tcaaatttta agggataatg tcaatgaatt
    20101 agaaagcta
//
LOCUS       DATRTN010000025        20009 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.025, whole genome shotgun sequence.
ACCESSION   DATRTN010000025 DATRTN010000000
VERSION     DATRTN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20009
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.025"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            13..642
                     /locus_tag="VBE22_000953"
     CDS             13..642
                     /locus_tag="VBE22_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7202423.1"
                     /translation="MQLNQRQERIIDIIKQFQPITGELIAEKLGVTRAALRSDLAVLS
                     MLGIIDAKPKVGYFYLGQYHASIGTSHFEKMTVSEIMGIPLTVHQKDSVYDVIVHIFM
                     EDAGCAFILDDDDFLCGVVSRKDLLKTSIGGGDLSKMPIGMVMTRMPHVTTVLENESL
                     FAAADKLVSRKVDSLPVVRHDKQYPEKFKVIGKLSKTILASLFLEIRDY"
     gene            653..1483
                     /locus_tag="VBE22_000954"
     CDS             653..1483
                     /locus_tag="VBE22_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEO7202424.1"
                     /translation="MEDQLTIFIISDSLGETAKAIAKACLSQFPGHDDWHFQCFSYIN
                     SQERLEQVFEEASQKTVFMMFSLVDVALASYAQKRCESEHYAYVDLLTNVIQGISRIS
                     GIDPLGEPGILRRLDNDYFKRVESIEFAVKYDDGRDPRGILQADLVIIGISRTSKTPL
                     SMFLADKNIKVINIPLVPEVPVPKELRMIDSRRIIGLTNSVDHLNQVRKVRLKSLGLS
                     STANYASLERILEETRYAEEVMKNLGCPIINVSDKAIEETATIILEILKTNGQVAKNL
                     "
     gene            1496..4141
                     /locus_tag="VBE22_000955"
     CDS             1496..4141
                     /locus_tag="VBE22_000955"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEO7202425.1"
                     /translation="METKFVYHFDEGCKEMKELLGGKGANLAEMTSIGLPVPQGFTIT
                     TQACNDYYDNACHIRESILSQIDQAMAQLEVEQNKQLGSVDDPLLVSVRSGAVFSMPG
                     MMDTVLNLGLNDRSVQGLVKKTEDERFAYDSYRRFIQMFADVVTGIPKYKFDTILDRL
                     KTDKCYQDDTELTGSDLQRLVEFYKELYQKEAGEKFPQDPKRQLLLAIEAVFKSWNNP
                     RAKIYRKLNDISETLGTAVNIQAMVFGNMGNNSGTGVAFTRNPSTGAANLFGEYLINA
                     QGEDVVAGIRTPQSISKLAEQMPIIYQEFVSVTQKLEAHYRDMQDMEFTIENGNLYML
                     QTRSGKRTAKAAIKIAVDQVNEGLISKEEAILRIEPKQLDQLLHPSFDLESLKKAILL
                     TTGLPASPGAAYGKVYFHAEDVVQEMKKGNPVLLVRQETSPEDIEGMVSANGIITARG
                     GMTSHAAVVARGMGKPCVAGCSQLLVDEVRREISIGHQTIKEGEMLSIDGATGNVYIG
                     QVPMAETSVDCDFEIFMKWVDENRDMMVCSNADNPRDAQKALDFGAEGIGLCRTEHMF
                     FDDERIPVVREMILADDVLSRRKALERLLSFQRDDFYQIFKVLKGKTCTIRLLDPPLH
                     EFLPHDKESIESMARQMGISTLAIEKRIQTLEEFNPMLGHRGCRLAITYPEIYQMQMR
                     ALVQGAILAMKEGYEAKPEIMIPLVSAHEEISIIRDLIEETIIEESKSKKINLSFPIG
                     TMIETPRACMIADDIAKFADFFSFGTNDLTQMSFGFSRDDAGKFLGEYVDKGLLKKDP
                     FQALDQKGIGRFIEQAVRLGKEVKPNLKIGICGEHGGEPSSIEFCYQQGLHYVSCSPF
                     RIPIAKLAAAQAKIKQSRNDITIDK"
     gene            4279..4581
                     /gene="gatC"
                     /locus_tag="VBE22_000956"
     CDS             4279..4581
                     /gene="gatC"
                     /locus_tag="VBE22_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEO7202426.1"
                     /translation="MKISEEEVRHVANLSKLRFSDQETKEFASSLSKIVDMIELLNEV
                     DTEGVPVTTTMADRKTVMREDIAQPGNNRDDLFKNVPQHQDYYIKVPAILEDGGDA"
     gene            4581..6047
                     /gene="gatA"
                     /locus_tag="VBE22_000957"
     CDS             4581..6047
                     /gene="gatA"
                     /locus_tag="VBE22_000957"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEO7202427.1"
                     /translation="MSFNNQSIDQLHDLLVKKEISATELTKATLEDIHAREQAVGSFI
                     TISDDMAIAQAKEIDDKGIDADNVMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATAVEKLYAKDMIVIGKANMDEFAMGGSTETSYFKKTNNAWDHSKVPGGSSGGSAAAV
                     ASGQVRLSLGSDTGGSIRQPASFNGIVGMKPTYGRVSRFGLFAFGSSLDQIGPMSQTV
                     KENAQLLTVISGHDVRDSTSSERTVGDFTAKIGQDIQGMKIALPKEYLGEGIAPGVKE
                     TIIKAAKHLEKLGAVIEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRTENY
                     KNLDDIYVNTRSEGFGDEVKRRIMLGTFSLSSGYYDAYYKKAGQVRSLIIQDFEKVFA
                     DYDLILGPTAPTTAFDLDSLNHDPVAMYLADILTIPVNLAGLPGISIPAGFDKGLPVG
                     MQLIGPKFSEETIYQVAAAFEATTDYHKQQPKIFGGEN"
     gene            6047..7489
                     /gene="gatB"
                     /locus_tag="VBE22_000958"
     CDS             6047..7489
                     /gene="gatB"
                     /locus_tag="VBE22_000958"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEO7202428.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPSSAHFGQEQNANTNVIDWSF
                     PGVLPVMNKGVVDAGIKAALALNMDIHQNMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGTRKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEEFGTKAELKNLNS
                     FNNVRKGLIHEEKRQAQVLRSGGQIQQETRRFDETTGETILMRVKEGSSDYRYFPEPD
                     LPLFDISDEWIDQVRLELPEFPQERRAKYVSSFGLSSYDASQLTATKATSDFFEKAVA
                     IGGDAKQVSNWLQGEVAQFLNSESKSIEEIGLTPENLVEMISLIADGTISSKIAKKVF
                     VHLAKNGGSAEEFVKKAGLVQISDPEVLIPIIHQVFADNEAAVIDFKSGKRNADKAFT
                     GYLMKATKGQANPQVALKLLAQELAKLKEE"
     gene            7619..8533
                     /locus_tag="VBE22_000959"
     CDS             7619..8533
                     /locus_tag="VBE22_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEO7202429.1"
                     /translation="MTKKEKGTMMTLAAGLAWGISGISGQYLMSHGVHVNLLTSLRLL
                     ITGIFLLSLARSKQKEHLVAAWKQPKFLKQVLLFSIFGLVLNQYAFLRAIHLTNAGTA
                     TVLQYMAPILILSIVCILNRQRPTSFEIIAIAMAILGTYMIATHGKLGSLAITPKGLM
                     WGLGSAITYSIYILLPVKLIHEWGSTIVIGSGMFIGGILFSLVTKAWQYPLQINVMSI
                     LAYIGIIGIGTIFAYTFFLKGVSIVGAVKGSLLASVEPVSSVFLTVLVLGEIFYPIDL
                     LGMLFIFLAVTLISYKDLIALKKMKRVK"
     gene            8617..9177
                     /locus_tag="VBE22_000960"
     CDS             8617..9177
                     /locus_tag="VBE22_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEO7202430.1"
                     /translation="MNSKIYFLEMKLSIDDYRPTFVVEAVYDLRAEDLLRHGIRAVLV
                     DLDNTLIAWNNPDGTAEVRAWLDEMTTADISVVVVSNNNHARVERAVSRFGVDFVSRA
                     MKPFTRGINMAIERYGFDRDEVIMVGDQLMTDIRASHRAGIKSVLVKPIVKSDAWNTK
                     FNRLRERRVWKKIEENYGKIVYQKGI"
     gene            9177..10295
                     /gene="yqeH"
                     /locus_tag="VBE22_000961"
     CDS             9177..10295
                     /gene="yqeH"
                     /locus_tag="VBE22_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEO7202431.1"
                     /translation="MEELFCIGCGARIQTENKDAAGYTPRAALEKGLETGELYCQRCF
                     RLRHYNEITDVHITDDEFLKLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFVAGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPVDVILTSAQNHHAIKDLIDTIEKY
                     RHGRDVYVVGVTNVGKSTLINAIIREITGSRDVITTSRFPGTTLDKIEIPLDDGSYIF
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNSEQTLFLAGLARFDFISGQKQ
                     GFTAYFDNNLNLHRTKLVGADEFYTKHVGKLLTPPTGKEVSDFPKLVRHEFTIKNKMD
                     IVYSGLGWIRVKSEAENPVVVAAWAPEGVAVVLRKALI"
     gene            10388..10705
                     /gene="yhbY"
                     /locus_tag="VBE22_000962"
     CDS             10388..10705
                     /gene="yhbY"
                     /locus_tag="VBE22_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEO7202432.1"
                     /translation="MLTSKQRAFLKSEAHSMKPIIQIGKNGLNDQIKTSVRNALDARE
                     LIKVTLLQNTDEDIHDVAEVLEDEIGCDTVLKIGRILILYKESARKENRKISVKVKAV
                     KSN"
     gene            10835..11467
                     /locus_tag="VBE22_000963"
     CDS             10835..11467
                     /locus_tag="VBE22_000963"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEO7202433.1"
                     /translation="MALELLTPFTKVELEEKKRDTNRKQIGIMGGNFNPVHNAHLVVA
                     DQVRQQLCLDQVLLMPEFQPPHIDKKETIDEQHRLKMLELAIEGIDGLSIEPIEIERK
                     GISYTYDTMKLLIEKNPDVDYYFIIGADMVEYLPKWHRIDELVKMVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRQFIKSNRQPNYLLPKEVLDYIRKEGLYK"
     gene            11464..12051
                     /gene="yqeK"
                     /locus_tag="VBE22_000964"
     CDS             11464..12051
                     /gene="yqeK"
                     /locus_tag="VBE22_000964"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEO7202434.1"
                     /translation="MTYKDYTGLDRTELLSKVRHMMSDKRFNHVLGVERAAIELAERY
                     GYDKEKAGLAALLHDYAKELSDDEFLRLIDKYQLDPDLKKWGNNIWHGLVGIYKIQED
                     LAIKDQDILAAIAKHTVGSAQMSTLDKIVYVADYIEHNRDFPGVEEARELAKVDLNKA
                     VAYETARTVSFLASKAQPIYPKTIETYNAYIPYLD"
     gene            12115..12636
                     /locus_tag="VBE22_000965"
     CDS             12115..12636
                     /locus_tag="VBE22_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO7202435.1"
                     /translation="MTCALLLIDIQQGIMDKKPKHLTNFAELLDDLLLSAKGSNCEVI
                     WIRHHDEELPQGSPQWEIWERRHLVTHHKIIDKTYNSCFKDTHLHDYLQSKHISQLIM
                     MGLQTEYCFDTSVKVAFEYGYDIFIPQGGHLTFDTPTLSGDSIKKHYENIWHHRFATM
                     VAKDNLLKLINEE"
     gene            12638..12994
                     /gene="rsfS"
                     /locus_tag="VBE22_000966"
     CDS             12638..12994
                     /gene="rsfS"
                     /locus_tag="VBE22_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEO7202436.1"
                     /translation="MTEKDLLQLVVKAADEKRAEDIVILDLQPVTSVADYFVIMSASN
                     SRQLEAIADNIREQVKGNGGDASHLEGDSKAGWVLLDLNSVVVHIFSEDERQHYNLEK
                     LWHEAPLLDAEDFMTE"
     gene            13060..13794
                     /locus_tag="VBE22_000967"
     CDS             13060..13794
                     /locus_tag="VBE22_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7202437.1"
                     /translation="MATYETFAAVYDAVMDDTLYDKWTDFSLRHFPKGKKKLLELACG
                     TGIQSVRFSQAGYAVTGLDLSGDMLKLAKKRATSAHQSIQFIEGNMLDLSNVGKYDLI
                     TCYSDSICYMQDEVEVGDVFIEVYKALEENGVFIFDVHSTYQTDKVFPGYSYHENADD
                     FAMVWDTYEDDAPHSIVHELTFFVQEEDGRFTRHDEVHEERTYDILTYDILLEQAGFK
                     DVKVYADFEDKKPTATSARWFFVAHK"
     gene            13817..14068
                     /locus_tag="VBE22_000968"
     CDS             13817..14068
                     /locus_tag="VBE22_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202438.1"
                     /translation="MIIFLYACLMIFGILYIIERRVSQKKSKVILGLLMNPKLHLDLL
                     SALKPFEKETDRLIYLRKTYHLDIIDAVTLNNQLKEKEV"
     gene            14065..15174
                     /locus_tag="VBE22_000969"
     CDS             14065..15174
                     /locus_tag="VBE22_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEO7202439.1"
                     /translation="MTVTGIVAEFNPFHNGHKYLLEQAQGIKVIAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVIELPFLVSVQSADYFASGAVSILARLGVDNLCFGTEEMLD
                     YDRIGDIYVNKKEEMEAFLKKQSDSLSYPQKTQAMWQEFAGIAFSGQTPNHILGLAYT
                     KAASQNGIRLNPIQRQGAGYHSSEKTEIFASATSLRKHQSDRFFVEKGMPNSDLFLNS
                     PQVVWQDYFSLLKYQIMTHSDLTQIYQVNEEIANRIKSQIRYVETVDELVDKVATKRY
                     TKARIRRLLTYILINAVESPIPNAIHVLGFTQKGQQHLKSVKKSVDIVTRIGSQTWDS
                     LTQRADSVYQMGNANIAEQTWGRIPFHQSVSQSDL"
     gene            15267..15980
                     /locus_tag="VBE22_000970"
     CDS             15267..15980
                     /locus_tag="VBE22_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7202440.1"
                     /translation="MKTVKEISHISGISVRTLHYYDEIDLLPPSFVGENGYRYYDDES
                     LIKLQEILLFKELEFPLKKIKEIMDSPNYDRNQALLDQIRWLELKKQRLEEVIEHAKS
                     IQRGKNMSDFTAYNQEELEAFQEEARTRWGDTDSYKEFENSYSKNDFSMISQAMSQIF
                     KDFGQLKELSPTDEKVQKQVQILQDYITAQFYNCTNDLLASLGIMYIQDERFQRSIDN
                     WGGQGTALFVSKAIDSYCQ"
     gene            16119..16523
                     /locus_tag="VBE22_000971"
     CDS             16119..16523
                     /locus_tag="VBE22_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO7202441.1"
                     /translation="MKGFHGNIEKLTLGNTNFRQVLYTAEHCQLVLMTLPVGGEIGSE
                     IHAENDQFFRFEAGHGKVVIDGNEYEVADGDAIIVPAGAEHNVINTSETEMLKLYTIY
                     SPAHHKDGIIRATREEAEENEEDFDGVTSETK"
     gene            complement(16789..17502)
                     /locus_tag="VBE22_000972"
     CDS             complement(16789..17502)
                     /locus_tag="VBE22_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO7202442.1"
                     /translation="MTQFLGEFLGTFILVLLGDGVVAGNVLSKTKEEGTGWTAIVFGW
                     GIACTVAVYVSGLFSPAHLNPAVTLAMASIGAISWGQVIPFIIAQMLGAMVAATILWL
                     HYYPHWKETKDSGLILASFSTGPAIRHTPSNLLGEIIGTAILVITIMAIGPSKVAAGL
                     GPIIVGIVIFAVGFSLGPTTGYAINPARDLGPRLMHAILPIENKGDSDWSYAWIPVVG
                     PIIGGVLGAILYNIALTLI"
     gene            complement(17512..17886)
                     /locus_tag="VBE22_000973"
     CDS             complement(17512..17886)
                     /locus_tag="VBE22_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEO7202443.1"
                     /translation="MSDIGIIVVSHSKNIAQGVVDLISEVAKDVSITYVGGTEDGEIG
                     TSFDQVQQIVEQNDKKTLLAFFDLGSAKMNLELVADFSEKNIIINSVPVVEGAYTAAA
                     LLQAGADLDSIQSQLAELTINK"
     gene            complement(17886..18464)
                     /gene="dhaL"
                     /locus_tag="VBE22_000974"
     CDS             complement(17886..18464)
                     /gene="dhaL"
                     /locus_tag="VBE22_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEO7202444.1"
                     /translation="MEVKTAIEWMHTFNQKIQSNKDYLSELDTPIGDGDHGGNMARGM
                     TAVIENLDNNEFSSAADVFKTVSMQLLSKVGGASGPLYGSAFMGITKAEQSESTISEA
                     LGAGLEMIQKRGKAELNEKTMVDVWHGVIEAIEKNELTEDRIDSLVDATKGMKATKGR
                     ASYVGERSLGHIDPGSFSSGLLFKALLEVGGV"
     gene            complement(18502..19491)
                     /gene="dhaK"
                     /locus_tag="VBE22_000975"
     CDS             complement(18502..19491)
                     /gene="dhaK"
                     /locus_tag="VBE22_000975"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEO7202445.1"
                     /translation="MKKILNQPTDVVTEMLDGLAYVHNDLVHRVEGFDIIARNEEKSG
                     KVALISGGGSGHEPSHAGFVGEGMLSAAVCGAVFTSPTPDQVLEAIKEADEGAGVFMV
                     IKNYSGDIMNFEMAQDMAEMEGIDVASVVVDDDIAVEDSLYTQGKRGVAGTILVHKIL
                     GHAARHGKSLQEIKAIADELVPNIHTVGLALSGATVPEVGKPGFVLAEDEIEFGIGIH
                     GEPGYRKEKMQPSKALATELVDKLIESFDAKSGEKYGVLINGMGATPLMEQYVFANDV
                     AKLLEDKGIEVNYKKLGNYMTSIDMAGLSLTLIKLENQEWLEALNSDVTTIAW"
     gene            19665..>20009
                     /gene="dhaS"
                     /locus_tag="VBE22_000976"
     CDS             19665..>20009
                     /gene="dhaS"
                     /locus_tag="VBE22_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO7202446.1"
                     /translation="MASSLITKKKIAKSFKRLFISQAFDKISVSDIMEDAGIRRQTFY
                     NHFVDKYALLEWIFQTELSEQVTDNLDYISGFQLLSELLTFFKMNQEFYIKLFQIEDQ
                     NDFSSYFESYCEQ"
BASE COUNT         6586 a         3189 c         4032 g         6202 t
ORIGIN      
        1 gaaggtgatc ttattcaatt aaatcaaagg caagaaagaa ttattgatat tatcaaacag
       61 tttcagccca ttactggtga gctaatagct gagaaattag gagtaacaag agcagcacta
      121 aggtctgatt tggcggtttt aagtatgcta ggtatcatag atgcaaaacc taaggttggt
      181 tatttttatt taggacagta tcatgcttca atagggacaa gtcattttga aaagatgaca
      241 gtttcagaaa ttatggggat ccctctgaca gttcatcaaa aagattcagt ttatgatgtt
      301 attgtacata tttttatgga agatgctggt tgtgctttta tcttggatga tgatgatttt
      361 ctctgtggag tcgtgtcacg taaagattta ttaaagacca gtattggcgg aggagatctt
      421 tctaaaatgc caataggaat ggtgatgaca cgtatgccac acgtgacaac tgttttagaa
      481 aatgaaagtc tttttgcggc agctgataaa ttagtgagca gaaaagtgga tagtctccct
      541 gtcgttcgtc atgataagca atatcccgaa aaatttaaag tgatcgggaa attatctaaa
      601 acaatcctcg ctagtttgtt tttagaaata agagattatt aggaggaaac tgatggaaga
      661 tcaactcact atatttatta tttcagattc tttaggggaa acagccaaag ctattgcaaa
      721 agcgtgtttg tcacaatttc caggacatga tgattggcat tttcaatgtt tttcctatat
      781 taacagtcag gaacgcttag aacaagtttt tgaagaggct agccagaaga cagtatttat
      841 gatgtttagt ttagttgatg ttgctttggc ttcctatgca cagaaacgat gtgaaagtga
      901 acattatgct tatgttgact tattaacaaa tgttattcaa ggaatatcaa ggatatcggg
      961 tatagatcct ttgggagaac cagggatttt acgacgctta gacaatgatt atttcaaaag
     1021 agtagaatca atagaatttg cagtaaaata tgatgatggg agagatccgc gaggtatttt
     1081 acaagcagat ttagttatta taggaatttc aagaacttct aaaacgccat taagtatgtt
     1141 tttagcagat aaaaatatta aagttattaa tattcctttg gttccagagg ttcctgttcc
     1201 taaagaatta cgtatgattg attctaggag aattatcgga ctaactaatt cggttgatca
     1261 tcttaatcaa gtccgaaaag ttcgattaaa gtccttaggt ctttctagta cggcaaatta
     1321 tgccagttta gaacggattt tggaggagac gaggtatgct gaagaagtta tgaaaaattt
     1381 aggttgccca atcattaatg tatctgataa agcaatcgaa gaaacagcaa cgattatttt
     1441 agaaatctta aaaacaaatg gacaagtagc taaaaatctg taaaaggaga cgctgatgga
     1501 aactaagttt gtgtatcatt ttgatgaagg ttgtaaggaa atgaaggaac tgctaggtgg
     1561 taagggagct aatctagctg aaatgacgag tattgggtta ccggtaccac aaggttttac
     1621 tattacaaca caggcttgta atgattatta tgataacgct tgccatatcc gagagagtat
     1681 tcttagtcaa attgatcaag caatggcaca attggaggtt gaacagaaca agcaattagg
     1741 aagtgttgat gatcctttgt tggtgtcagt tcggtcagga gcagtttttt cgatgcctgg
     1801 tatgatggat acagttttaa atttagggtt aaatgatcgt agtgtccaag gtttagtgaa
     1861 aaagacagaa gacgagcgtt ttgcttatga cagttatcgt cgttttatcc aaatgtttgc
     1921 agatgtggtt acaggcattc caaaatataa atttgatacc attttagatc gacttaagac
     1981 agataaatgt tatcaagatg acacagaact aactggttca gatttacaga gacttgttga
     2041 attttacaaa gaactatatc aaaaagaagc aggagaaaaa tttccgcaag atcctaaaag
     2101 gcagcttttg ttagcaattg aggcagtttt taaatcatgg aataatccaa gagctaagat
     2161 ttatcgtaaa ttaaatgata tttcggagac gcttggaaca gccgttaata ttcaagctat
     2221 ggtttttggg aatatgggaa ataacagcgg aacgggagtt gcttttacac gaaacccatc
     2281 aactggagca gcaaatttat ttggtgagta tttgattaat gcacaaggag aagatgttgt
     2341 tgcaggtatc agaacaccac aatccatatc aaagttggca gaacaaatgc ctattattta
     2401 ccaagagttt gtaagcgtta cccaaaagtt agaagctcat tatcgcgata tgcaagatat
     2461 ggagtttaca attgaaaatg gtaatcttta tatgcttcag acgagaagcg gtaagcgtac
     2521 agcgaaggca gctatcaaaa tagcagtaga tcaagtaaat gaaggcctaa ttagtaaaga
     2581 agaagctata ctacgtatcg agcctaaaca attagatcag ttattacacc cttcttttga
     2641 cttggaaagt ttaaagaaag ctattctttt gacgacagga cttccagcct ctcctggagc
     2701 tgcttatggg aaagtttatt tccatgcaga agatgttgtt caagagatga aaaagggaaa
     2761 tccagtgcta ttggtcagac aagagacatc acctgaagat atcgagggga tggttagtgc
     2821 aaacgggatt ataacagcaa gaggcggaat gacctcccat gcggcagtag tagccagagg
     2881 aatggggaaa ccatgcgttg caggctgtag tcaattattg gttgatgaag taagacgaga
     2941 aatttctatt gggcatcaaa cgataaaaga aggtgagatg ttatcaatcg atggtgctac
     3001 aggaaatgtt tatataggac aagttccgat ggcagagact agcgtagatt gtgattttga
     3061 gatatttatg aaatgggtag atgaaaatcg agatatgatg gtgtgttcaa atgcagataa
     3121 tccgagagat gctcaaaagg ccttggactt tggagcagaa ggaataggtc tttgtcgcac
     3181 ggaacatatg ttttttgacg atgaacgtat tccagtcgtt agagaaatga ttttagcaga
     3241 tgatgtattg agtcgaagga aggctctaga aaggctttta tctttccaaa gagacgattt
     3301 ttatcaaatt tttaaggtat tgaaaggtaa aacttgtacg attcgtttgc ttgaccctcc
     3361 tttacatgaa ttcttacccc atgataaaga gtctatcgaa tctatggcaa gacaaatggg
     3421 gatatctact ttagctattg aaaaacggat tcaaacatta gaagaattca accctatgtt
     3481 aggtcatcgt ggttgtagac ttgcgattac ctatccggaa atttaccaaa tgcagatgag
     3541 agctcttgta caaggcgcta tacttgcaat gaaagaggga tatgaggcaa agccagaaat
     3601 tatgattcct ttagtgtcag cgcatgaaga aatctctata atacgtgatc tgattgaaga
     3661 gacaattatt gaggagagta aatctaaaaa aattaactta tcatttccta ttgggacaat
     3721 gattgaaaca ccaagagctt gtatgatagc ggatgatatt gcaaagtttg cggatttctt
     3781 tagttttggg accaatgatt taacacaaat gagctttgga ttttcacgag atgatgctgg
     3841 aaaattctta ggagaatacg ttgataaagg acttctaaaa aaagaccctt tccaagcttt
     3901 agatcaaaaa ggaataggaa ggtttattga gcaggcagtg aggttaggaa aagaagttaa
     3961 gccaaacctt aaaataggta tctgtggaga gcatggtgga gaaccatcat ctattgagtt
     4021 ctgttaccaa caggggttac attatgtatc gtgttcgccg tttagaattc ctattgctaa
     4081 attagcggct gcacaggcta aaatcaaaca aagtagaaat gatataacaa tagataaata
     4141 aacagtaaac gcttcttatc ataggtaaga agcgtttatt atgaaaagct gggataatgt
     4201 gataacattg agagtttaat atgctataat agtagagact atcttaaaac gaaaatgata
     4261 cagatataag gagaactcat gaaaatttct gaagaagaag tgcgtcatgt tgccaatctg
     4321 tcaaaattgc gcttttctga tcaagagact aaagaatttg cgtcaagttt gtcaaagatt
     4381 gttgatatga ttgaactctt aaatgaagtt gacacagaag gcgtaccagt aacaacgacc
     4441 atggcagatc gcaaaaccgt gatgcgtgaa gatattgctc aaccaggcaa taatcgtgat
     4501 gacttattta aaaatgtacc acagcatcaa gattattata ttaaagtacc tgcaattctt
     4561 gaggatggag gagatgccta atgtcgttta ataatcaatc aattgatcag ttgcatgatc
     4621 ttctcgttaa aaaagaaatt tcagcaactg aattaacaaa agctacattg gaagatattc
     4681 atgcgcgtga acaagcggtt ggatctttta ttactatttc tgatgatatg gcaatagctc
     4741 aagccaaaga aattgatgat aaaggaattg atgctgataa tgttatgtca ggtattccct
     4801 tagcagttaa agataatatt tcaacaaagg ggattcttac aacagctgct tctaaaatgc
     4861 tctataacta tgagccaatc tttgatgcta cggctgttga aaagttatat gctaaagata
     4921 tgattgtgat tggtaaagca aacatggatg aatttgccat gggaggctct acagaaacat
     4981 cttattttaa aaagacaaat aacgcttggg accatagtaa agttccggga ggatcatcag
     5041 gtggttctgc agcagcagta gcttcaggac aagtacgttt gtcattgggc tctgatacag
     5101 gtggttcaat tcgccagccg gcttcattta atggaatcgt tggtatgaag ccaacttacg
     5161 gacgtgtatc acgctttggt ttgtttgctt tcggtagttc gcttgaccag attggaccga
     5221 tgtctcaaac agtaaaagag aatgcacagc ttttgacagt tatttctggg catgatgtaa
     5281 gagattcaac ttcttctgag cgtactgtag gcgattttac tgctaaaatt ggtcaagata
     5341 ttcaaggaat gaaaattgct ttaccaaaag aatatttggg agaaggaata gcgccaggag
     5401 ttaaagaaac gattattaag gccgcaaaac atctggaaaa actaggtgca gttattgagg
     5461 aagttagttt accgcattct aaatacggtg ttgctgttta ttatattatt gcttcatcgg
     5521 aagcttcttc aaacctacaa cgttttgatg gtattcgtta tggataccgt acagaaaatt
     5581 ataaaaactt ggatgatatc tatgtcaata cacgatctga aggttttgga gacgaagtta
     5641 agcgtcgcat tatgctggga acgttcagtt tatcttctgg gtattacgat gcttattata
     5701 aaaaagcagg tcaagttcgt agtttgatta ttcaagattt tgaaaaagtt ttcgcagatt
     5761 atgacttgat tttaggtcca acagctccaa caacagcctt tgacttagat tctttaaatc
     5821 acgatccagt tgcaatgtac ctagctgata ttctaacaat accagttaat ttggctggtt
     5881 taccaggtat ttcaatccct gctggttttg ataaaggtct accggttggt atgcagttga
     5941 ttggtccgaa attcagtgaa gaaacaatat atcaggttgc tgctgctttc gaagcaacga
     6001 cagattatca taaacaacaa cctaaaattt ttggaggtga gaactaatga attttgaaac
     6061 agtcattgga cttgaagtcc atgttgaatt aaatacgaac tctaaaattt tctcaccttc
     6121 ttccgctcat tttggtcagg aacaaaatgc taatacaaat gtaattgact ggtctttccc
     6181 aggtgtcctt ccagtcatga ataaaggagt tgttgatgca ggaatcaagg cagcacttgc
     6241 cttgaatatg gatatccatc agaacatgca ttttgaccgt aaaaattatt tctatccaga
     6301 taatcctaaa gcttatcaaa tttcacagtt tgatgagcct attggttaca atggttggat
     6361 tgaaatcgaa ttagaagatg gtacacgaaa aaaaattcgt attgagcgtg cccacctcga
     6421 agaagatgca ggaaaaaata cacatgggac agatggttat tcctatgttg accttaatag
     6481 acaaggtgtt ccactgattg aaattgtatc agaagctgat atgcgttctc ctgaggaagc
     6541 ttatgcttat ttgacagctc tcaaagagat tatccaatat actggaattt ctgacgttaa
     6601 aatggaagaa ggttctatgc gtgtagatgc caatatttca cttcgtcctt atggtcaaga
     6661 agagtttggt actaaagcag aacttaagaa ccttaactca ttcaataatg tacgtaaagg
     6721 tttgatacat gaagaaaaac gtcaagcaca agttttacga tctggtggtc aaattcagca
     6781 agagactcgt cgttttgatg aaacaacagg tgagactatt ttgatgcgtg tcaaggaagg
     6841 ctcatcagat taccgctatt tccctgaacc agatctacca ctatttgata tctcggatga
     6901 atggattgat caagttcgtt tagaattacc agaatttcca caggaacgtc gtgcgaaata
     6961 tgtatcgtct tttggtctat catcttatga tgctagccaa ttaacagcta caaaagcaac
     7021 ttctgatttc tttgaaaaag ctgttgctat tgggggtgat gcaaaacaag tttccaattg
     7081 gttacaagga gaagtggcgc aatttcttaa cagtgaatca aaatcaatcg aagaaattgg
     7141 tctgactcct gagaatttgg ttgagatgat tagcttgatt gctgacggta caatttcatc
     7201 caaaattgct aaaaaagttt ttgtgcattt agctaaaaat ggtggttcag ctgaagaatt
     7261 cgttaaaaag gctggtttag ttcaaatttc agatcctgaa gtattaatcc caattattca
     7321 tcaagtcttt gcagataatg aagctgctgt gattgacttt aagtcaggaa aacgcaatgc
     7381 tgataaggcc ttcacaggtt atctaatgaa agcaacaaaa ggacaagcta atccacaagt
     7441 tgctctgaag ctgttagcgc aagaattagc aaaattaaaa gaagaataga aatcagcctt
     7501 ttggctggtt tttattcgct aatttagcac gaacgcctag ggataaataa ttaagaagct
     7561 aagatattta tcagatgact ctaactacag tgtttaagaa ataaaaatga ggggaaaaat
     7621 gactaaaaaa gaaaaaggaa caatgatgac cttagctgcc ggactagcat ggggcatttc
     7681 tggaattagt ggtcaatact tgatgtcgca cggagtacac gttaaccttt tgacttcttt
     7741 acgattgttg ataacaggta tctttctctt gtctttagct agatcaaaac aaaaggagca
     7801 tttagtagca gcgtggaaac agccaaaatt tttaaagcag gttttacttt tttctatatt
     7861 tggtttagtt ttaaatcaat atgcttttct cagagcgatt catttaacaa atgctggaac
     7921 tgcaacggtt ttgcaatata tggcaccaat acttattttg agtattgttt gtatcctaaa
     7981 cagacaaaga ccaacatctt ttgaaataat agcaattgct atggcaattt taggaaccta
     8041 tatgattgca acgcatggga aattaggaag tttagctatc acaccgaaag gattaatgtg
     8101 ggggcttggt tcagccatta cttattctat ttatatttta ttacctgtaa aacttattca
     8161 tgaatgggga agtacgattg ttattggttc aggaatgttt ataggtggga ttttatttag
     8221 tttggtgaca aaagcgtggc aatacccctt acaaattaat gtgatgagta tacttgctta
     8281 tattggcatt attggtattg ggaccatttt tgcttacact tttttcctta aaggggttag
     8341 tattgtaggt gctgtcaagg gtagcttatt agcatcggta gagccagttt cttcggtgtt
     8401 tttgacagta cttgttttag gagaaatttt ttatccaata gatttacttg ggatgttgtt
     8461 tattttcctt gctgtaactc taatctctta caaagattta attgcattga agaaaatgaa
     8521 acgagtaaaa tagtatcttt tggggttgaa accctaattt aaaggtcttt aaagaccttt
     8581 tttctttact cgctatctct tgagaattat ggtagaatga atagtaaaat atattttttg
     8641 gagatgaaat tgagtataga tgattatagg ccgaccttcg tggttgaggc agtttatgat
     8701 ttaagagcag aagatttatt acgtcatggc attcgtgcgg ttttagttga tttagataat
     8761 acactcattg cttggaataa tccagatggt actgctgagg tgcgtgcatg gttagatgag
     8821 atgacaacag cggatatttc agttgtagta gtgtctaaca acaatcatgc tcgcgtagaa
     8881 cgtgcagtat cacgttttgg tgtggatttt gtaagtcgtg cgatgaaacc atttactcgt
     8941 ggtataaaca tggcaattga gcgttatggc tttgatcgtg acgaggtgat tatggttgga
     9001 gatcaattga tgacagatat tcgtgctagt catcgcgcag gaataaagtc cgtattggta
     9061 aaaccaattg ttaaatctga tgcttggaat accaagttca atcgcttacg tgaacgtcgc
     9121 gtttggaaaa aaatagaaga gaattacggt aaaatcgtat atcaaaaagg aatttaatgg
     9181 aagagttatt ttgtattggc tgcggagcca ggattcaaac tgaaaataaa gatgctgctg
     9241 gctatacacc tcgtgcagca cttgaaaaag gtttagagac aggagaatta tactgtcagc
     9301 gttgtttcag attgcgccac tataatgaga ttactgacgt tcatatcact gatgatgaat
     9361 ttttgaaatt actacatgag gttggagata gtgatgccct tgtggtgaat gtcattgata
     9421 tttttgattt caatggttca attattcctg gtcttagtcg tttcgttgca ggaaacgatg
     9481 ttcttttagt aggaaataaa aaagatattc ttcccaaatc ggttaaagat ggaaaagtaa
     9541 cacaatggtt aactgagaga gctcatgaag aggggcttcg tcctgttgat gtcatcttga
     9601 caagtgccca aaatcatcat gctattaagg atttgattga tacgattgaa aagtatcgtc
     9661 atggtcgaga tgtttatgtt gttggggtta ctaatgttgg aaagtcaact cttattaacg
     9721 ctattattcg tgaaatcact ggtagtcgtg atgttattac gacctcacgt ttcccaggta
     9781 caacattaga taaaatagaa atacctcttg atgatggtag ctatattttt gataccccgg
     9841 gtattattca tcgtcatcaa atggctcatt atttgacagc aaaaaatctt aaatatgtta
     9901 gtcctaaaaa agaaataaaa cccaaaactt atcagcttaa ttcagaacaa acattattct
     9961 tggctggcct agcacgtttt gattttattt ctggtcaaaa acaaggtttt acggcatatt
    10021 ttgataataa cctgaattta caccgcacta aactagttgg agcagatgaa ttttacacta
    10081 aacatgttgg aaaattatta acaccaccaa caggtaagga agtttcagat tttcctaaat
    10141 tagttcgtca tgaatttaca atcaagaata aaatggatat tgtttattcg ggccttggtt
    10201 ggatacgtgt gaaatcagaa gcagaaaatc cagtagtagt agctgcttgg gctccagaag
    10261 gcgtggcagt tgttttacgc aaagctttaa tttaataact aaattaacca gcaacatatg
    10321 acgatttttc atctaattta actgatgata cagtcataat cctataatag ataaaaagga
    10381 aactaatatg ttaacatcta aacaacgtgc tttcttaaaa tcagaagcac attctatgaa
    10441 acccattatt caaattggga aaaatggttt aaatgatcaa atcaaaacaa gcgtacgtaa
    10501 tgcactcgat gcacgtgagc taatcaaagt aaccctttta caaaataccg atgaagatat
    10561 ccacgatgtc gctgaagttc tggaagatga aattggctgc gatactgtcc ttaaaatagg
    10621 acgtatcttg attctttata aagagtcagc gcgcaaagaa aatcgtaaaa tttcagttaa
    10681 agtaaaagca gtaaaatcaa actaaactac aatagattaa gaaatagagt atagaactaa
    10741 aaaacgattt acctaagtta cacttccgta tgatagctga tcatgataat cagtgtttgt
    10801 ttttttagag atactacttg ttcatggagg aattatggca ctcgaattgc taacaccatt
    10861 tacaaaagtt gaattagaag agaaaaagcg agatactaac agaaaacaaa ttggaattat
    10921 gggaggaaat tttaaccctg ttcataacgc ccaccttgtt gttgcagatc aagtacgtca
    10981 gcaattgtgt ttagaccaag ttctgttgat gcctgaattt cagccaccac atattgataa
    11041 aaaagagaca attgatgaac agcatcgtct caagatgctt gaattagcaa tagaaggcat
    11101 tgatggttta tctattgaac cgattgaaat tgaacgtaaa ggtatcagct atacatacga
    11161 tacgatgaag cttctaattg aaaaaaatcc agatgttgat tattatttca ttattggggc
    11221 agatatggtg gaatatctac ctaagtggca tcgtattgat gaacttgtta aaatggtgca
    11281 gtttgttggt gttcaacgtc caaaatataa agctggtaca tcatatcccg ttatttgggt
    11341 ggatctgcca ttgatggata tctcatcaag tatgattcgt caatttatta aaagtaatcg
    11401 tcagcccaac tatttactgc ctaaagaggt attagattat atcaggaaag aaggtcttta
    11461 caaatgacct acaaagatta cacaggttta gatcggactg aacttttgag taaagtgcgt
    11521 catatgatgt ccgacaagcg ctttaatcat gtgttaggag tagagcgtgc tgcaatagaa
    11581 ttagcagaac ggtatggtta tgataaggaa aaagcgggcc tagctgcttt attacatgat
    11641 tatgctaaag aactatctga tgatgaattt cttagattaa ttgataaata ccaactagat
    11701 cccgacctaa aaaaatgggg taataatatt tggcatggtc ttgttggcat ttataaaata
    11761 caagaggatc tagctatcaa agatcaagat attttagctg ctattgcaaa acatactgtt
    11821 ggttcagccc aaatgtccac attggataaa atcgtttatg tagcagatta cattgaacac
    11881 aatcgagatt ttccaggagt tgaagaagct cgtgagttag cgaaagttga ccttaataaa
    11941 gcagtagctt atgagactgc tagaacagtt tcgtttttag caagtaaggc tcagcctatc
    12001 tatcctaaaa cgattgaaac ctataatgct tatatacctt atctcgacta attcttttat
    12061 tgaaataatg gtcatgatag gtttggcgat gcagtggtca ttttggagga aagtatgact
    12121 tgtgcacttt tactaattga tatccaacaa ggtattatgg ataagaaacc aaagcattta
    12181 acaaattttg cagagctttt agatgattta cttttaagtg ccaaaggttc taattgtgaa
    12241 gtgatatgga taaggcatca tgatgaggaa ttgcctcaag gttctccaca gtgggaaatt
    12301 tgggaacggc gacatcttgt gacgcatcac aaaataattg ataaaactta caatagttgt
    12361 tttaaagata ctcatttaca tgattacttg caaagtaagc atattagtca attgatcatg
    12421 atgggacttc aaacagaata ctgttttgat acatcagtaa aagttgcttt tgaatatggt
    12481 tatgatatct ttattccaca aggtggacac ctaacatttg atacaccaac tttaagtggg
    12541 gattctatta aaaaacatta cgagaatatt tggcatcatc gttttgcgac aatggtagca
    12601 aaagataact tattgaaatt aataaacgag gaataaaatg acagaaaaag acttactaca
    12661 gctagtcgtt aaggctgcag atgaaaaacg agcagaagat attgtaattt tagatttaca
    12721 gcctgtaacg agtgttgcag attactttgt gattatgagt gcaagtaata gtaggcagtt
    12781 agaagcaatc gcagacaata tccgtgaaca agtaaaagga aatggtgggg atgctagcca
    12841 ccttgaaggc gatagtaaag ctggttgggt acttcttgac ctcaatagcg tcgtggttca
    12901 tatcttctct gaagatgaga gacagcacta taaccttgaa aaactttggc atgaagcacc
    12961 attgcttgat gctgaagact ttatgacaga ataaagctaa gcagtcttgg gactgctttt
    13021 tgagagatgt aatatcaaga aagatttgag aactattata tggcaacgta tgaaactttt
    13081 gcagctgttt atgatgctgt catggatgat actttatatg ataaatggac tgatttttca
    13141 cttcgtcatt ttccaaaagg taagaaaaaa cttttggaat tagcatgtgg tacgggaatt
    13201 cagtctgttc gattttcaca agctggttat gctgttacag gacttgatct tagtggcgat
    13261 atgctgaaac ttgccaaaaa acgtgcaact tctgctcacc aaagtatcca gtttattgaa
    13321 ggaaatatgt tagatttatc taatgttggt aaatatgatc tcatcacgtg ctattcagac
    13381 tctatttgtt atatgcaaga tgaagtcgag gttggtgatg tttttatcga agtctataag
    13441 gccttagaag aaaatggcgt ctttattttt gatgttcatt cgacttatca aacagataaa
    13501 gtatttcctg gttattctta tcatgaaaat gcagatgact ttgctatggt gtgggatact
    13561 tatgaagatg atgcacctca ttctattgtc catgaattaa cattttttgt tcaagaagaa
    13621 gatggtcgat ttacacgtca tgatgaagtt catgaagagc gtacttatga tattttgact
    13681 tatgatattt tacttgaaca ggcaggtttt aaagatgtta aggtgtatgc agattttgaa
    13741 gataaaaaac caactgcaac ttctgctcgc tggttttttg tagctcataa ataagtaata
    13801 atttggaggt aaaaagatga taatattctt atacgcttgt ctgatgatat ttgggatact
    13861 ttatattatt gaacgacggg taagtcagaa aaagtctaaa gttattttgg gattattgat
    13921 gaaccctaaa cttcatttgg atttattatc ggcattaaaa ccatttgaaa aagaaacaga
    13981 tcgtctcata taccttcgta aaacttatca cctagatatt attgatgctg ttactttaaa
    14041 taatcaattg aaagagaaag aagtatgaca gttacaggaa ttgttgctga atttaatcct
    14101 tttcataatg gccataagta ccttttagaa caagctcaag gaataaaagt aatagccatg
    14161 tcgggtaatt ttatgcaacg tggtgagccg gcgattgtag ataaatggac ccgtgcccag
    14221 atggcactag aaaatggtgc tgatttagtg attgaactcc cttttttggt ttcggtacag
    14281 tcagcggatt atttcgctag tggagctgtg tctatcctgg cacgattagg tgtggataac
    14341 ttatgcttcg gtaccgaaga aatgcttgat tatgaccgta ttggggatat ttatgtgaat
    14401 aaaaaagagg agatggaagc ttttttgaaa aaacaatcgg attctctttc ctatccacaa
    14461 aaaacgcaag ctatgtggca agaatttgca gggattgcat tttcgggaca aaccccaaat
    14521 cacattttag gcttagccta caccaaagca gctagccaaa atggtattcg attaaatccg
    14581 atacaacgtc aaggagctgg ttatcattca tcagaaaaaa cagagatatt tgcttccgca
    14641 acaagtcttc gtaaacatca atctgatcgt ttctttgtag aaaaaggtat gcctaacagt
    14701 gacttgtttt tgaatagtcc acaagtagtt tggcaagatt attttagttt actcaaatat
    14761 cagataatga cgcattctga tttgacacaa atttatcaag tgaatgaaga gatagccaat
    14821 cgtataaaaa gtcaaattcg ttatgtggag actgtggatg agttagttga taaagtcgca
    14881 actaaacggt ataccaaagc aagaattcga cgtttattaa cctatatctt gataaatgct
    14941 gtggaaagtc ctataccaaa tgctattcat gtcttaggtt ttacacaaaa aggtcaacag
    15001 catttaaagt ctgtgaagaa gtctgtggat atcgtgacga ggattggttc acaaacgtgg
    15061 gatagtttga cgcaacgagc agattctgtt tatcagatgg gaaatgctaa tatagcagag
    15121 caaacatggg gacgtatccc ttttcatcaa tctgttagtc aaagcgacct ttgatttaag
    15181 ctttataaat taatcatcac aaaataatat ttttcccttg acccttacgt aacgttatac
    15241 attatactct ttttagagag gttattatga aaacagttaa agaaataagt cacatttctg
    15301 gtattagtgt gcgcacactt cattattatg atgaaataga tttgttacct ccttcatttg
    15361 taggagagaa tggttatcgt tactatgatg atgaatcact gattaagtta caagaaattt
    15421 tgttatttaa ggaactggaa tttcctctta aaaaaataaa agagataatg gatagtccaa
    15481 attatgatcg taatcaagca cttcttgatc aaattaggtg gctggaacta aagaaacagc
    15541 gactagaaga agtaattgaa catgctaagt ctatccagag gggtaaaaat atgtcagatt
    15601 ttacagctta taatcaagaa gaattagaag catttcaaga ggaagctaga acacgctggg
    15661 gggatacaga tagttacaaa gaatttgaaa atagctatag taagaatgat ttttcaatga
    15721 tttcacaagc tatgagtcag atttttaaag actttggaca gttgaaagaa ctttctccga
    15781 cagatgagaa agttcaaaaa caagttcaga ttttacaaga ttatatcaca gctcagtttt
    15841 acaactgcac taacgattta ttagcaagtc ttggaataat gtacatccaa gatgagcgtt
    15901 tccaaaggtc tattgataat tggggaggcc agggaacagc attatttgtt tctaaagcta
    15961 tagatagcta ttgtcagtag gctatatttt aggtttgcaa tgaaatgata ggaatcaagg
    16021 gggagtagat attattaaaa atgtcagact cttatctaaa tttttgacag taagtggcaa
    16081 aaagagtaga ctgtaggcat agaaaggtag gtaccgttat gaaaggattt catgggaata
    16141 tcgaaaaatt gacactagga aatacaaatt tccgccaagt cctctatacc gcagaacatt
    16201 gtcaactcgt tttaatgaca ctgcctgttg gtggagaaat tggtagcgag attcatgcag
    16261 aaaatgatca gtttttccgt tttgaagctg gtcatggtaa ggttgtcatt gatggtaatg
    16321 aatatgaagt tgcagatgga gacgctataa tagttccggc aggtgctgaa cataacgtaa
    16381 ttaacacttc tgaaaccgag atgttaaaac tctatacaat ttacagtcca gctcatcaca
    16441 aagatggtat tatccgtgcc acaagagaag aagctgaaga aaatgaagaa gattttgatg
    16501 gagttacttc tgaaactaaa taatactaga ctaaaaagcg ctatgtcaat agcgcttttt
    16561 agcttaacag tagtcatttc ttggagaata taaatagtaa tttaggaagt aatgttaaat
    16621 agccgagctt ttaaggaaac gttaagtaag ggcggttata ctataaacat cctaaacttt
    16681 tttcataaaa tctccagaaa gaacactaag tattttggtg ttttttctat tttgaaagca
    16741 tactgcaact acgaaaaacc agaaagttaa tactttctgg ttttttagtt aaattaaagt
    16801 taaagcaata ttgtataaga tagcgccaag gacaccgccg ataattggac ctacaactgg
    16861 aatccaagcg tatgaccaat ctgagtcgcc cttattttca ataggtaaga tagcatgcat
    16921 aagacgaggc cctaggtcac gtgcagggtt aatagcataa ccagttgttg gaccaagaga
    16981 gaatcctact gcgaaaataa ctataccaac gataattgga ccaagacctg ctgcaacttt
    17041 actaggacca attgccataa ttgtaatcac aagaatagca gtaccgataa tttcaccaag
    17101 gagattcgat ggtgtatgac ggatagcagg tccggttgag aagctggcaa ggataagtcc
    17161 actatctttt gtttctttcc aatgaggata atagtgtaac cataaaatcg ttgccgcaac
    17221 cattgctcct aacatctgtg caatgataaa ggggataact tgtccccaag aaattgctcc
    17281 aatagaagcc attgctaatg tgactgctgg atttagatga gcaggactaa ataatccaga
    17341 aacataaaca gcaacggtac aagcaattcc ccaaccaaaa acaatagcag tccatccagt
    17401 tccttcttct ttggttttac tgagcacatt tcctgcaact acgccatccc caagaagaac
    17461 caaaataaat gttcctaaaa attccccaag aaattgagtc ataatatctc cttatttatt
    17521 gatagttaat tcagctaatt gtgattgaat actatccagg tcagcacctg cctgtagaag
    17581 agcagcagcg gtgtaagctc cctccactac tgggacagag ttaatgataa tatttttttc
    17641 tgaaaaatca gctactaact ctaaattcat ttttgctgaa cctaaatcaa agaaagctaa
    17701 taacgtcttt ttatcatttt gctctacaat ctgttgaact tgatcaaaag aagtaccaat
    17761 ctcaccatct tctgttccac ccacgtaagt tattgagaca tcctttgcta cctctgaaat
    17821 aagatcaaca actccttgag caatattttt agagtgagaa acaacaataa taccaatatc
    17881 agacattaga cacctccaac ttctagtaat gctttaaata ataatcctga agaaaatgac
    17941 cctggatcaa tgtgtcctaa tgagcgttct ccaacataag aagcgcgtcc ttttgttgct
    18001 ttcatccctt ttgtagcatc tactaaggaa tcaatacggt cttcagtcaa ttcatttttt
    18061 tcaatcgctt caataacacc gtgccaaaca tcaaccattg ttttttcatt aagttcagct
    18121 ttaccacgtt tttgaatcat ttcaagtcct gcgccaagtg cctctgaaat ggtactttca
    18181 ctctgttcag cttttgtaat tcccataaat gcagaaccat agagtggtcc agatgctccg
    18241 cctactttgc ttaaaagttg catggatacg gttttgaaaa catcggcagc actactaaat
    18301 tcattgttat caaggttttc aataacagca gtcataccac gcgccatatt gccaccatgg
    18361 tcgccatcac ctattggagt atctagttcg gataaatagt ccttgttgga ttgaattttt
    18421 tggttaaaag tgtgcatcca ttctattgca gtttttactt ccataagagt tcctttcttt
    18481 tgttctaatt aacaggaatg attaccaagc aatggttgta acgtcactat taagtgcttc
    18541 taaccactct tgattttcca gtttaatcaa ggttagtgat aaaccagcca tatcaattga
    18601 agtcatatag ttaccaagtt ttttgtaatt cacttcaatg cctttatctt caaggagttt
    18661 ggcaacatcg ttagcaaaga catattgttc cataagtggg gttgcaccca taccgttaat
    18721 aaggacacca tatttttcgc cagatttagc atcgaacgat tcaattaact tatcaactaa
    18781 ttctgtcgct aaagcctttg aaggttgcat tttttcttta cgataacctg gttcaccatg
    18841 aataccgata ccaaattcaa tttcatcttc tgcaagaaca aatcctggct tgcctacttc
    18901 aggaacagta gccccgctta gtgcaagtcc aacagtgtga atgtttggta ctaattcatc
    18961 agcaatagct ttgatttcct gaagtgattt gccatgacga gcagcatgtc ctagaatttt
    19021 atggacaagt atcgtaccag ctacaccacg ttttccttgt gtgtaaagac tgtcttcgac
    19081 tgcaatatca tcatcaacga ccacacttgc aacgtcaatg ccttccattt cagccatatc
    19141 ttgtgccatt tcaaagttca tgatatctcc tgaataattt ttgataacca tgaaaactcc
    19201 agctccctca tcagcttctt taattgcttc aagaacttgg tcaggagttg gagaagtaaa
    19261 tactgcacca cacacagctg cagataacat tccttcgcct acgaaaccag catgtgaagg
    19321 ctcatgtcca cttcctccgc cagaaatgag agccactttt cctgacttct cttcattgcg
    19381 agcaatgata tcaaaacctt ctacgcgatg tactaaatca ttatgaacat atgctaatcc
    19441 atctagcatt tcagtaacaa catcagttgg ttgattgaga atttttttca tttggaaact
    19501 ccttttatgt ttaattctct tttattataa aatttgtaag ccttttccgg aatggacaga
    19561 ttttcttgtt tgtccgcttt gttagggttt tcaataatca taaatcatgt tacactaaac
    19621 cataataaat catcagcaat tcaatataaa ataaaggaga aattatggct tcttcgctta
    19681 ttactaagaa aaaaatagcc aagtcattta aacgtttatt tatttcgcaa gcttttgaca
    19741 aaatatcggt gagtgatatt atggaagatg caggtattag acgtcaaaca ttttataatc
    19801 actttgttga taaatatgct ttattagaat ggattttcca aacagaatta agcgaacaag
    19861 ttacggataa tttagactat atctcaggtt ttcagttgtt atcagagttg ttgacttttt
    19921 ttaaaatgaa tcaagaattc tatattaaat tatttcaaat tgaagatcaa aatgattttt
    19981 cttcgtattt tgagtcttac tgtgagcag
//
LOCUS       DATRTN010000026        19535 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.026, whole genome shotgun sequence.
ACCESSION   DATRTN010000026 DATRTN010000000
VERSION     DATRTN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19535
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.026"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..153)
                     /locus_tag="VBE22_000977"
     CDS             complement(<1..153)
                     /locus_tag="VBE22_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEO7202447.1"
                     /translation="MIYKIIASLFLVLIPIFSQVLVKIFKLKKFNIMFPDVAFPIFVF
                     LIPLISS"
     gene            complement(214..1119)
                     /locus_tag="VBE22_000978"
     CDS             complement(214..1119)
                     /locus_tag="VBE22_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7202448.1"
                     /translation="MKNQQKLMYLESIELYSNITKAAAHLFISQPYLSKVIKQLENEL
                     KIKLIQSQGHQTFLTYAGQRYLFYLKEIDMIERQMAKELYLIRSDKKGEITLGINSGL
                     ASSILANVLPKFNLEHPEISVKLLENNQNISEQLVASGDIDLAVGMAPIVYKDGIAST
                     TIYRDELFLMIPTTSQLYNAEKRGQIIPFEYPISVLDNEPLILTPLEYGIGKTIAQFY
                     ELHHMSLNQMITTSTVPTAASLSLSGMGATFVPQTLIHRYLDKECNVYHFHKNKLFSE
                     YIMIYKKDVELSGIALLLYKAFLTK"
     gene            1378..2592
                     /locus_tag="VBE22_000979"
     CDS             1378..2592
                     /locus_tag="VBE22_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO7202449.1"
                     /translation="MANASLRHQLFEKLDQKCDQMVAIRRYLHENPELSFKETKTAAY
                     ISDFYKGKDCHVQTQFGGMNGVVVDIYGDKATDKPIKHIALRADFDALPIQEETGLSF
                     ASKTAGVMHACGHDAHTAYLLILAESLIELKSEFSGHIRILHQPTEEVPPGGAKAMIE
                     AGCLDGIDAVLGIHVMSTMEEGTVQYHAGPIQTGRATFKVILQGKGGHGSMPHRANDT
                     IVAASSFVIAAQTIVSRRVNPFDTAVVTIGSFDGKGSANVIKDSVTLEGDVRVMSEET
                     RGVVEEEFKRILDGIAQTYGVSYQLDYQNDYPVLVNNSEVTQKVANSLKSVAIKEILD
                     VIDCDPQTPSEDFAYYAQTIPACFFYVGAHEEGQPYYPHHHPKFQIAESSLMVSAKSM
                     ATAALAMLEEGE"
     gene            2600..3982
                     /locus_tag="VBE22_000980"
     CDS             2600..3982
                     /locus_tag="VBE22_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7202450.1"
                     /translation="MLTTKENFKGNNKLLVGIVLAVLSFWLFAQSILNMGPDVQSSLG
                     ISSGAMDIGVSSTALFSGLFIVVTGGLADKLGRVKFTFIGLCLNIIGSLLIVLANGAV
                     LFIMGRIFQGLAAAFIMPSTMALVKTYYDGKDRQRAVSFWSIGSWGGSGLCSYFGGAV
                     ASTLGWRYVFIFSIIASVVSFLLILGTPESKNVGQKTHFDYLGLIIFIISMLSLNIGI
                     SMAQEHGLMNVIPLSLFTVMLIGFVLFYYVETRKSNSFIDFHLFENRFYLGATISNFL
                     LNAVAGTLIVINTYMQQGRQLTPKVAGEMSLGYLVCVLIAIRVGEKILQRFGARKPML
                     LGAMSTFVGIFLMTLVNIQGPLYLVLVFVGYALFGTGLGIYATPSTDTAISSIPNEKV
                     GSASGIYKMASSLGGAIGVATSIAIYHAFSGNADFHKAALCGLILNLVFCSLSILSIL
                     FVIPKKIEDK"
     gene            4193..4618
                     /gene="rplK"
                     /locus_tag="VBE22_000981"
     CDS             4193..4618
                     /gene="rplK"
                     /locus_tag="VBE22_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEO7202451.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGEPNKTKVATVTR
                     AQVQEIAETKMPDLNAANLESAMRMIEGTARSMGFTVTD"
     gene            4722..5411
                     /gene="rplA"
                     /locus_tag="VBE22_000982"
     CDS             4722..5411
                     /gene="rplA"
                     /locus_tag="VBE22_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEO7202452.1"
                     /translation="MAKKSKNLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     SYNLNIDVKKADQQIRGAMVLPAGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KIQGGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDDAKLVDNFKAFNDVIVKAKPATAKGTYITNLSITTTQ
                     GVGIKVDPNSL"
     gene            5801..7417
                     /gene="nikA"
                     /locus_tag="VBE22_000983"
     CDS             5801..7417
                     /gene="nikA"
                     /locus_tag="VBE22_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HEO7202453.1"
                     /translation="MRRNILLSITCLLMVTLTACHSQDSKSHKLNSDKLTLAWGEDFG
                     DVNPHRYNPDQFVIQDMVYEGLVRYGDNGKIEPALAESWSISQDGKTYTFKLRNAKYS
                     DGSNFNAANVKRNFDSIFSKSNRGNHNWFNLTNQLENYRALNQSTFEIKLKQAYSATL
                     YDLSMIRPIRFLSDSAFPKGDDTTKKNVKKPIGTGQWVVKSKKQNEYITFKRNENYWG
                     KKPKLKEVTVKVIPDAQTRALAFESGDVDLIYGNGIIGLDTFAQYTKDKKYVTAISQP
                     MSTRLLLLNAKETIFQDKKVRQAMNHAIDKVSIAKNTFRGTEKPADTIFSKSTPHSDA
                     KLNPYSYNVDKANQLLDQAGWKMGKNKVREKDGKALTLRLPYIATKATDKDLVTYFQG
                     EWRKIGINVSLIAMEEDDYWANAKKGNFDMMLTYSWGAPWDPHAWMSALTAKADHGHP
                     ENIALENLATKTEMDRLIKSALVDPKEENVDRDYKKVLELLHDEAVYIPLTYQSVISV
                     YRKGDFKTMRFAPEENSFPLRYIEKNNVSK"
     gene            7404..8348
                     /locus_tag="VBE22_000984"
     CDS             7404..8348
                     /locus_tag="VBE22_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7202454.1"
                     /translation="MYLSSSIIKKILSAFLALFFISLLTFILIKLSTVNPAENYLRLS
                     KISVSPEALKEAEHYLGLDKPLWKQYWLWFQKALTGDFGYSYVLRLPVLDLVLQRFLA
                     TLFLGTSAFLLIVTISTPLGVWAGLHESSRSDYLIRFLSFSSVSMPSFWVAYLLMLLF
                     SAKLNLLPVSGGNDLQSLILPSITLSFSTVGQYIALIRKAISQENRSLNVENARLRGV
                     KERYIVTHHLLRNALPAIMTALSLTWVYLLTGSIIVEEIFSWNGIGRLFVTSLRTSDL
                     PVIQACMLIFGTLFLANNFMTQCLMNWVDPRLRKSREK"
     gene            8348..9154
                     /locus_tag="VBE22_000985"
     CDS             8348..9154
                     /locus_tag="VBE22_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7202455.1"
                     /translation="MSKRIYFSSSLLVLLVISAIFAPILSSFDPQYVDLSQKLLAPNN
                     VHLLGTDQLGRDVLSRLLYGARYSLFLAIIISLLELTIGMFVGLIVGWYQGKLENLFL
                     WIANIILAFPSFLLSLATVGILGHGLGNLIFAIVFVEWVYYAKLMTNLVKSAKKEPYV
                     INAQIMGLSVWHILRKHIFPFVYQPILVMVLMNIGNIILMISGFSFLGIGVQPNITEW
                     GMMLHDARGYFRTATWMMLSPGIAIFLTVFSFNTLGDAIDKKDWKRQWNS"
     gene            9142..9930
                     /locus_tag="VBE22_000986"
     CDS             9142..9930
                     /locus_tag="VBE22_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202456.1"
                     /translation="MEQLEIRKLSLQIGEVPVLRDFSCKIGMGESLTIIGESGSGKTL
                     LAKLLVGHIPQGMTVRGNIFFKGVDLGKLTVKQWQKLRGRDIAYLVQNPMSMFNPFQK
                     IEAHILETILSHEKCSKRVALSKALEWMKRLNLDDAISLLKKYPFELSGGMLQRIMLA
                     TILSLDPQVIILDEPTSAVDCHNCSTISAILQELQNNGKTLITVTHDYQLARDLGGQL
                     LVISEGEVVEQGQTQTILSNPQHNYTKALTVQMEYEGDILNVGL"
     gene            9917..10597
                     /locus_tag="VBE22_000987"
     CDS             9917..10597
                     /locus_tag="VBE22_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202457.1"
                     /translation="MLVCNHVGKTFGRQEVLKDCHFHLKRGEIIGIMGKSGSGKSSLA
                     RLIIGLDSPTCGSIHFQGKNYTPKDGRAQIILVFQDALSSVNPYFSIEEILNEAFYGK
                     KITFELCQILEAVGLDGTYLKYKARQLSGGQLQRVCIARALLLKPKIIIFDESLSGLD
                     PVTQIKMLHLLQKIKRRYELSFIMISHDPKICQAICNRVFLIKNGYLVEDNEFLKRAC
                     STNCLTNL"
     gene            10718..11446
                     /locus_tag="VBE22_000988"
     CDS             10718..11446
                     /locus_tag="VBE22_000988"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEO7202458.1"
                     /translation="MEPKYQRILIKLSGEALAGDKGVGIDIPTVQSIAKEIAEVHNSG
                     VQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQYGVDTRVQT
                     AIPMQTVAEPYVRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEAEAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHVEVIKRGLKIMDATASTISMDNDIDLVVFNMN
                     ETGNIKRVVLGEQIGTTVSNKASE"
     gene            11462..12019
                     /gene="frr"
                     /locus_tag="VBE22_000989"
     CDS             11462..12019
                     /gene="frr"
                     /locus_tag="VBE22_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEO7202459.1"
                     /translation="MTKEIVTKAQERFEQSHQSLSREFAGIRAGRANASLLDRIQVEY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERAINESDLGINPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKIAIRNIRRDAMDEAKKQEKNKEITEDDLKSLEKDI
                     QKATDDAVKHIDEMTANKEKELLEV"
     gene            12137..12991
                     /gene="cvfB"
                     /locus_tag="VBE22_000990"
     CDS             12137..12991
                     /gene="cvfB"
                     /locus_tag="VBE22_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEO7202460.1"
                     /translation="MNTLLATVITGLVTDENKDFYFIQKDGFTFALSKSEGEHHIGEM
                     VKGFAYTDMQQKARLTTKETFATRDHYGWGTVTEVRKDLGVFLDTGLPDKQVVVSLDV
                     LPELKELWPKKGDRLYVCLDVDKKDRLWALPADPEVFQRMATPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYSEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKKIKQ
                     DQLGTELL"
     gene            13129..13638
                     /gene="msrA"
                     /locus_tag="VBE22_000991"
     CDS             13129..13638
                     /gene="msrA"
                     /locus_tag="VBE22_000991"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO7202461.1"
                     /translation="MERAIFAGGCFWCMVQPFEELDGIESVLSGYTGGHVENPTYKEV
                     CSKTTGHTEAVEIIFNPEKISYANLVELYWAQTDPTDAFGQFEDRGDNYRPVIFYENE
                     EQRQIAQKSKDKLQASGRFDRPIVTSIEPADTFYPAEDYHQAFYRTNPARYALSSARR
                     HAFLEENWH"
     gene            13635..13850
                     /locus_tag="VBE22_000992"
     CDS             13635..13850
                     /locus_tag="VBE22_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEO7202462.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADYAFDETTFPKHSSDFETV
                     SRYLEDEASFSFNLTDFDDIWEDYLNH"
     gene            14008..15780
                     /locus_tag="VBE22_000993"
     CDS             14008..15780
                     /locus_tag="VBE22_000993"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEO7202463.1"
                     /translation="MRYTNGNFEAFARPRKPEGVDKKSAYIVGSGLAGLAAAVFLIRD
                     GQMDGQRIHIFEELPLSGGSLDGVKRPDIGFVTRGGREMENHFECMWDMYRSIPSLEV
                     PDASYLDEFYWLDKDDPNSSNCRLIHKQGNRLESDGDFTLGTHSKELVKLVMETEESL
                     GAKTIEEVFSKEFFESNFWTYWATMFAFEKWHSAIEMRRYAMRFIHHIGGLPDFTSLK
                     FNKYNQYDSMVKPIISYLESHNVDVQFDSKVTNISVDFKNGQKLAKAIHLTVGGEAKT
                     IDLTPDDFVFVTNGSITESTNYGSHDTVAKPNTDLGGSWNLWENLAAQSDEFGHPKVF
                     YKDIPKESWFVSATATIKDPAIEPYIERLTHRDLHDGKVNTGGIVTVTDSNWMMSFAI
                     HRQPHFKEQKENETIVWIYGLYSNVEGNYIKKPIEECTGREITEEWLYHLGVPEMKIH
                     DLSDKQYVSTVPVYMPYITSYFMPRVKGDRPDVIPQGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYTFLNIERGVPEVFNSAFDIRVLLQSLYYLNDKKSVEDMDLPIPAL
                     MRKVGMKKIRGTYLEELLREAHLL"
     gene            15904..16890
                     /locus_tag="VBE22_000994"
     CDS             15904..16890
                     /locus_tag="VBE22_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEO7202464.1"
                     /translation="MQEYSIEITLQHPDDMMSLFGSNERHLKLIEENLDVIIHARTER
                     VQVLGDSEEAVETARLTIEALLVLVNRGMTVNTSDVVTALSMAQNGSIDKFVALYEEE
                     IIKDSYGKPIRVKTLGQKIYVDSVKNHDVVFGIGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTSRLMEREIIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDVSQIDLPKNVKS
                     GLIDAVEKLRNIKKIDFIHLSAKDVVRHPVVAEIINAYSDSESSHKLQSDKD"
     gene            complement(16981..17670)
                     /locus_tag="VBE22_000995"
     CDS             complement(16981..17670)
                     /locus_tag="VBE22_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HEO7202465.1"
                     /translation="MYVYCAATPDITSRKRILNKHCHYIELNNHYWELEDNGTLNDGT
                     DIDILYRNIDNFLSDLEDVVEHHNSRIGYTTCFWHNLINCQILYDPENQLQSLKERFE
                     VSYPSQLQKQIIIQNRNLLTGKLPSYDKQIIKALKRQDFVSTHHRTTAFLDSYFDIIF
                     ALNKLTHPGEKRMISYAKKNATLLPKHFEENIIKLCHHNSNEHTVKETLNDIIMHLDV
                     MLKENFQHLIG"
     gene            17976..18452
                     /locus_tag="VBE22_000996"
     CDS             17976..18452
                     /locus_tag="VBE22_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO7202466.1"
                     /translation="MKQDYISYIRSKVGHETIFLTYSGGILTDGKGRVLLQLRADKNS
                     WGIIGGCMELGESSVDTLKREFFEETGLRVEPIRLLNVYTNFQDSYPNGDKAQTVGFI
                     YEVSCPKPVNIEGFHNEETLQLDYFSKEDVKNITIVNEQHQLILDEYFSQTFQMGR"
     gene            18774..>19535
                     /locus_tag="VBE22_000997"
     CDS             18774..>19535
                     /locus_tag="VBE22_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7202467.1"
                     /translation="MKITLHGVAETLLITLYIRAKDAMAKHPILNDQKSLAIVEQIEY
                     DFDKFDNSEASFYATLARIRVMDREIKKFIRENPNSQILSIGCGLDTRFERVDNGQIR
                     WYNLDLPEVMEIRKLFFEEHERVTNIAKSALDETWTREVNPQNAPFLIVSEGVLMFLK
                     EDDVETFLHILTNSFSQFMAQFDLCHKEMINKGKQHDTVKYMDTEFQFGITDGHEIVD
                     LDPKLKQINLINFTDEMSKFELGTLRSLLPTIRKFN"
BASE COUNT         6388 a         2914 c         3898 g         6335 t
ORIGIN      
        1 agatgatatt aatggaatta aaaaaacaaa aataggaaaa gcaacatctg gaaacataat
       61 gttaaatttt ttaagtttaa atattttgac aagaacctgt gaaaatatag ggattaaaac
      121 aagaaataaa gaagcaataa ttttgtatat cataggtcta ttttataccg attttggtaa
      181 gatgtaaact gattttcaat agaattagcg aaactacttg gttaaaaaag ctttataaag
      241 taggagagca attccagata attcaacatc ttttttataa atcataatgt actctgaaaa
      301 taacttattt ttatgaaaat ggtagacatt gcactcttta tccaaatagc gatgaatgag
      361 agtttgtgga acaaatgttg ctcccatccc tgacaaagat aggctagctg ctgtaggaac
      421 ggtactagtt gtaatcatct gatttaatga catgtgatga agctcataga attgtgcaat
      481 tgttttgcca attccgtact ctaatggtgt taggataaga ggttcattgt ctagaactga
      541 aataggatat tcaaaaggaa taatctgtcc acgtttttca gcgttgtaga gttggctggt
      601 ggtaggtatc attaaaaaca attcgtcgcg gtaaatagtg gttgatgcaa ttccatcttt
      661 ataaacgata ggagccatcc caacagctag gtcaatatca ccactcgcta ccaattgttc
      721 agaaatattt tgattgtttt cgagtaattt gacagaaatt tctgggtgct ccaagttaaa
      781 ttttggaagt acatttgcca gaatagaact tgctaagcca gagttaatcc caagtgtgat
      841 ttctcctttt ttatcagaac ggatgagata tagttctttg gccatttgac gctcaatcat
      901 atcaatttct tttaggtaga atagatatct ctgtccagca taagttaaaa aggtttggtg
      961 cccttggctc tggatgagtt ttatttttaa ctcattttct aattgcttga tcactttgct
     1021 taggtaaggt tgagaaataa aaaggtgtgc agcggcttta gtgatgtttg agtatagttc
     1081 gatactctct agatacatta atttttgttg atttttcaag actttataac cagtttgata
     1141 tccttagatt aataataagt tatgatacgt tatggctata agtttactat cattttgagc
     1201 taaaatcaat ctattttaat ttagtagtat aacatgatat attcataact atttagttat
     1261 agaaatatct aaaaaaattt atttgtaaaa aattattttc aaaagtagac tatataagag
     1321 cagattgtta gaataatagc cttattttaa ctgttcatat tttaaggagg aaagcaaatg
     1381 gccaatgcat ctttgcgaca tcaattattt gaaaaacttg accaaaaatg tgatcaaatg
     1441 gttgctattc gtcgctattt acatgaaaat cccgaattat catttaaaga gacaaaaaca
     1501 gcagcttata tttcagattt ttataaaggt aaggactgtc atgttcagac acaatttggt
     1561 ggtatgaatg gcgtcgtagt tgatatctat ggtgataaag cgacagataa gcccatcaaa
     1621 cacattgctt tgagagccga ctttgatgct cttccaattc aggaagagac agggctttct
     1681 tttgcttcaa aaacagcggg cgtcatgcat gcttgtggac atgatgccca tacagcttat
     1741 ttattgattt tggctgagag cttgattgaa ttaaaatcag aattttcagg tcatatccgt
     1801 atcttacatc aaccgactga agaggtgcca ccgggcggag caaaagcaat gattgaagca
     1861 ggatgtttag atggcattga tgctgttctt ggtatccatg ttatgtcaac gatggaagag
     1921 gggacggtac aatatcacgc aggtcccatt caaacagggc gtgccacttt taaagttatt
     1981 ttgcaaggta agggtggaca tggttccatg ccacatcgtg caaatgatac aattgttgca
     2041 gcttctagtt ttgttatagc agcgcaaacg attgtcagtc gtcgagttaa tccatttgac
     2101 acagcagtag ttactatcgg atcttttgat ggtaaaggtt ctgctaatgt gatcaaagat
     2161 agtgtaacct tagaaggtga tgtgcgcgtg atgtctgaag agacacgagg tgttgttgag
     2221 gaggaattta agcgtattct agatggaata gcacaaactt atggtgttag ttatcagtta
     2281 gactatcaga atgattatcc tgttttagtt aataacagtg aagtgacaca gaaagttgca
     2341 aatagtttaa aatctgttgc tataaaggaa attttagacg tcattgattg cgatcctcaa
     2401 acgccgtctg aagattttgc ctattatgct caaacaatcc cagcttgctt cttctatgtt
     2461 ggagcacacg aagaggggca accatattac ccacatcatc atccaaaatt ccaaattgca
     2521 gaatcatcat tgatggtatc tgctaaatca atggcaactg cagcgcttgc tatgcttgaa
     2581 gaaggagaat agataacata tgttaacgac aaaggaaaat ttcaaaggaa ataacaaatt
     2641 attggtggga attgtactcg ctgtactatc attttggtta tttgcacagt ctattttaaa
     2701 catgggacca gatgttcagt catcattagg tatttcatct ggtgcaatgg atattggtgt
     2761 ctcaagtacg gcattgtttt caggtttatt tattgttgta actggtggtt tagcagataa
     2821 acttggtcga gttaaattta cttttattgg tctttgctta aatattattg gttctctact
     2881 aattgtttta gctaatggag cagtgctatt tattatggga cgtatttttc aaggattggc
     2941 agcagctttt attatgccat caacaatggc cttggttaaa acgtactatg atggtaaaga
     3001 tagacaacgt gcagttagtt tctggtcaat cggatcatgg ggtggctcag gtctatgttc
     3061 ttattttggt ggagcggtgg caagtactct aggttggcgt tatgtcttta tcttttcaat
     3121 catcgcatca gtagttagtt ttctattaat tttaggaacc ccagaaagta aaaatgttgg
     3181 acaaaaaact cactttgatt accttggttt aattattttt attatttcaa tgttatcgtt
     3241 aaatattggg atatctatgg ctcaagaaca tggattgatg aacgttattc cattgagtct
     3301 ttttacagtg atgcttattg gatttgttct gttttattat gttgaaacac gtaaatctaa
     3361 tagttttatt gactttcatc tgtttgaaaa tcgattttac ttaggtgcga caatatcaaa
     3421 tttcttactg aatgcagttg caggaactct gattgttatt aatacttaca tgcaacaagg
     3481 tcgccagcta acaccaaagg ttgcaggtga aatgtcttta ggctatttgg tttgtgtact
     3541 aattgctatt cgagttggtg agaaaatttt acaacgcttt ggtgcccgta agccaatgct
     3601 tttgggagct atgagtacat ttgttgggat atttttaatg actttggtca atattcaagg
     3661 ccctttgtat cttgtgttag tctttgttgg ttatgcttta tttgggacag gcttggggat
     3721 ctatgcgaca ccatcaacgg atacagctat ttctagtatt cctaatgaaa aagtaggttc
     3781 tgcatctgga atttacaaaa tggcaagttc actaggtggt gcaattggtg tagcaacttc
     3841 aattgcgatt tatcatgctt tttctgggaa tgcagatttt cacaaagcag ctttgtgtgg
     3901 attaatttta aacctagttt tttgtagtct atcgatctta tcaattcttt ttgttatccc
     3961 taagaaaatt gaggataaat aaataacaat acttgagatt caaaatctca agtattgttg
     4021 taataatgta aaaggaattg ggaaagaaat ttaaaatata atgaaattaa tggcttcaaa
     4081 ctattgcttt tatttatgat tatagtataa tggtaaggta tgttataaac atacctgtgg
     4141 gaggtaaaaa tttctaatta ccgccaaaac cacaacagga ggatttttaa aaatggctaa
     4201 aaaagttgaa aaacttgtaa aacttcaaat tcctgctggt aaagcaactc cagctccacc
     4261 agttggacca gcacttggtc aagcaggaat caacatcatg ggattcacta aagagtttaa
     4321 cgctcgtaca gctgatcaag ctggtatgat tattccagtt gttatctcag tatatgaaga
     4381 caaatcattt gatttcatca ctaaaacacc accagctgct gttcttttga aaaaagctgc
     4441 aggtgttgaa aaaggatcag gtgaacctaa caaaactaaa gttgcaacag ttactcgtgc
     4501 gcaagtacaa gaaattgctg aaactaaaat gccagattta aacgctgcaa accttgagtc
     4561 tgcaatgcgt atgatcgaag gtactgctcg ttctatggga ttcactgtta ctgactaatc
     4621 agaaacaccc acataacccg caagacttca tcgtagtttg atgagtgacg tgggagattt
     4681 taaatcaaat cgattgacca cattacaagg agaattttaa aatggctaaa aaaagtaaaa
     4741 acttacgtgc tgctcttgag aaaattgaca gcactaaagc atacagcgta gaagaagctg
     4801 tagctcttgc aaaagaaact aactttgcta aatttgatgc aactgttgaa gtttcttata
     4861 accttaacat tgacgttaaa aaagctgacc aacaaatccg tggtgctatg gttttaccag
     4921 caggtactgg taaaacttca cgtgttcttg tttttgctcg tggtgctaaa gctgaagaag
     4981 ctaaagcagc aggtgcagac tttgtcggtg aagatgactt agttgctaaa atccaaggtg
     5041 gatggcttga ctttgatgtt gttatcgcaa caccagatat gatggcactt gttggacgtc
     5101 ttggacgtgt ccttggacct cgtaacttga tgccaaatcc taaaactggt acagtaacta
     5161 tggacgttgc taaagcagtt gaagagtcta aaggtggtaa aattacttac cgtgctgata
     5221 aagcaggtaa cgtccaagcc cttattggta aagtttcatt tgatgatgct aaattagttg
     5281 ataacttcaa agcatttaat gatgttattg ttaaagctaa accagctact gcaaaaggaa
     5341 cttacatcac aaacctttca attacaacaa cacaaggtgt tggtatcaaa gttgatccta
     5401 actcacttta atcaataaaa aaactaggaa ttccttccta gttttattta ttctatatac
     5461 ttaactggtt aagtatatat tttaataatc aaaggatatt atatgtgttt cttttgaaaa
     5521 atagcttttt atatgtatta accttcttaa agactaatga atgtgtgatg tgttttaagt
     5581 aatagatgag aaaaaggaaa aatgaaattg gcgatatggt atgtgactat tatgattatt
     5641 ctttagcaat aatctattaa aatgagttcg cagggaataa gtaaattttg tatcgttgta
     5701 gattttttat tttaagattc ttgacaagac attactaaaa tgatttaatt aagtttgctt
     5761 aaaaaaataa ttaattaaac ttaaaagaaa gttggtgaaa atgagacgta acatattatt
     5821 gagcattact tgtttattga tggtgactct gactgcatgc cattcacagg atagcaaaag
     5881 tcataaacta aacagtgata aacttacgct tgcttgggga gaagactttg gtgatgttaa
     5941 tccgcatcgc tataaccctg atcagtttgt tatccaggat atggtttatg aggggttggt
     6001 acgatatggt gacaatggga aaattgaacc agctttagcg gaaagctgga gcattagtca
     6061 agatggaaaa acctacactt ttaaattgag aaatgctaaa tattctgatg gcagtaactt
     6121 taatgctgcg aatgtcaaac gtaattttga tagtattttt tcgaagtcta atagaggtaa
     6181 tcataattgg tttaatttaa cgaatcaatt ggaaaattat cgtgccttga atcaaagtac
     6241 ttttgaaatt aaactgaaac aagcttatag tgctacttta tatgatctct ctatgattcg
     6301 accaattcgc tttttatctg atagcgcttt ccctaaaggt gatgatacaa ctaagaagaa
     6361 tgtaaaaaaa ccaattggaa cgggtcaatg ggttgtgaaa agtaaaaagc aaaatgaata
     6421 tattaccttc aaacgtaatg agaattactg gggaaagaaa ccaaaattaa aagaagtaac
     6481 cgtaaaagtt atacccgatg ctcagacgcg tgctttagca tttgaatcag gtgacgttga
     6541 tttgatctat gggaatggca ttattggttt agacactttt gcccaatata cgaaggacaa
     6601 gaaatatgtc acagctattt ctcaacctat gtcaacaaga cttttgttgc taaatgctaa
     6661 ggaaactatt ttccaagata aaaaagttcg tcaggcaatg aatcatgcta ttgacaaggt
     6721 ttctattgct aagaacactt ttagaggaac agaaaagcca gcagacacta ttttttcaaa
     6781 atcaacacct cactcagatg ctaagttaaa tccttatagc tataatgttg ataaagcaaa
     6841 tcagctattg gatcaagctg gttggaagat gggaaaaaat aaggttcgtg aaaaagatgg
     6901 taaagctcta accttacgtc ttccttatat cgccactaag gcaactgata aagacctggt
     6961 aacttatttc caaggagaat ggcgtaaaat cggtattaat gtttctctta ttgctatgga
     7021 agaagatgat tattgggcta atgctaaaaa gggtaatttt gatatgatgt taacctattc
     7081 ttggggagca ccatgggacc ctcatgcttg gatgtcggca ttaactgcca aggcagatca
     7141 tggacatcca gaaaatatag ctttagaaaa tctagcaact aaaactgaaa tggatagact
     7201 tattaagtca gctctagtag atcctaagga agaaaatgtc gatagagact ataagaaggt
     7261 tcttgaatta ttgcatgatg aggctgtata tatcccatta acttatcaat cggtgatttc
     7321 tgtttatagg aagggtgatt ttaaaaccat gcgttttgcg ccagaagaaa attcattccc
     7381 attacgctat atagaaaaaa acaatgtatc taagtagttc gatcatcaaa aaaatattgt
     7441 cagctttttt ggcattgttt ttcatatcac ttctaacatt tattcttatc aaactatcaa
     7501 cagttaatcc agcagaaaat taccttcgtt tatcaaaaat aagtgttagt ccagaagcac
     7561 taaaagaagc agagcactat ctaggtttag ataagccatt atggaagcag tactggctgt
     7621 ggttccaaaa agcattaaca ggagattttg gatattctta tgtgctcagg ttgccggtat
     7681 tagatttggt tttacaacgg tttttggcta ctttatttct aggcacgagt gccttcttgc
     7741 taattgttac catttctacc ccgttaggtg tgtgggctgg cttacatgaa tcttctcgga
     7801 gtgattactt gattcgattt ttgagttttt ctagtgtgtc tatgccaagt ttttgggttg
     7861 cttacttgtt gatgctgcta ttttcggcaa aattaaatct tctgcctgtt tcaggaggta
     7921 acgatttaca aagtttaatt ttgccaagta ttaccttaag tttttctacc gtaggtcaat
     7981 atatagccct tattcgaaaa gctattagtc aagaaaatag aagtttaaat gtggaaaatg
     8041 ctcgattaag aggagtaaaa gaacgttata ttgtcacaca tcacctcctt agaaatgcct
     8101 tacctgcgat aatgacggca cttagcttga cttgggttta cttattaaca ggatcaatta
     8161 ttgttgaaga aattttttca tggaatggaa taggacgttt atttgtgacc agtctaagga
     8221 cttcagatct tccagtaata caagcttgta tgctaatttt tggaacctta tttttagcta
     8281 ataattttat gacacagtgt cttatgaatt gggttgatcc tcggttacga aagtcaaggg
     8341 agaaataatg tcaaaaagaa tttatttttc gagtagctta ctggtattgt tagtgattag
     8401 tgccattttt gcccctattc tatctagctt tgatccacaa tacgtagatt tatcacaaaa
     8461 attattggct cccaataatg ttcatttgtt aggaactgac caattgggta gagatgtgtt
     8521 atctcgcttg ctttatggtg ctagatattc actgttttta gctattatta ttagcttatt
     8581 ggaattaacg attggtatgt ttgtgggtct tattgttggt tggtatcaag gaaagttaga
     8641 aaacctcttt ttatggatag ctaatattat tttagctttt ccgagctttt tgctctctct
     8701 tgcaaccgtg gggatcttag gtcatggtct agggaattta atttttgcaa ttgtctttgt
     8761 ggaatgggtt tactatgcta aattaatgac caatttagtt aagagtgcaa aaaaagagcc
     8821 ttatgtgata aatgcgcaaa ttatgggact ttcggtctgg catattttaa gaaaacatat
     8881 ttttcctttt gtatatcagc caatcctcgt tatggtcctt atgaatatag gaaatattat
     8941 tttaatgatt tctggctttt ctttcttagg gattggtgtg caacctaata tcacagaatg
     9001 gggaatgatg ttgcacgatg ctagaggata ttttaggaca gctacttgga tgatgttatc
     9061 tcctggaatt gcgatttttt taacagtatt ttcctttaat actttaggcg atgccataga
     9121 taaaaaagat tggaaacgac aatggaacag ttagaaataa ggaaattgtc attacagata
     9181 ggagaggtcc ctgtactaag agattttagt tgtaaaatag gtatgggaga atcgttaact
     9241 attattggtg agagtggttc agggaaaacc ctcttggcaa aattattggt tggtcatatt
     9301 ccacaaggta tgacagttag aggaaacata ttttttaagg gagttgattt aggtaaacta
     9361 actgtaaagc agtggcagaa attaagggga cgagatattg cctatttagt acaaaatccc
     9421 atgtctatgt ttaatccttt tcaaaaaatt gaagcgcata ttttggaaac aatccttagt
     9481 catgagaaat gttcaaagag agtagcctta tctaaagcgt tagaatggat gaaacgttta
     9541 aatttagatg atgcgatatc cctgttaaaa aaatacccct ttgagcttag tggaggaatg
     9601 ctacaaagaa ttatgttagc cactatatta tctcttgatc ctcaggtaat catattagat
     9661 gagccgacct cagcggttga ttgtcataat tgctctacta tatcagctat tttgcaggaa
     9721 ttgcaaaata atggaaaaac cttgattact gtaacgcatg attatcagtt agctagagac
     9781 ctgggggggc aattactagt tataagtgag ggagaagttg ttgaacaggg acaaacccaa
     9841 actattttaa gcaatcctca acataactat acgaaagcac tgacagtgca aatggagtat
     9901 gaaggagata ttcttaatgt tggtttgtaa tcatgtcgga aaaacctttg gacgacaaga
     9961 ggttttaaag gactgtcatt ttcatcttaa gagaggagaa ataattggta taatgggtaa
    10021 aagtggcagt ggtaaaagta gccttgcccg actcattata ggactagata gtcctacttg
    10081 tggttcgata cattttcaag gaaaaaatta cacacctaaa gatggtaggg cgcagattat
    10141 ccttgttttt caagatgcgc ttagttcggt caatccatat tttagtattg aggaaatttt
    10201 gaatgaagct ttttatggaa aaaaaataac ttttgaatta tgccaaatat tagaagcggt
    10261 gggtttagat ggaacttatc taaaatataa agctagacaa cttagtggtg gtcagctaca
    10321 gagagtttgt atcgctagag ctttactctt aaagcctaaa attattattt ttgatgaatc
    10381 cttaagtgga ttagacccag taactcaaat taaaatgcta catttattac aaaaaataaa
    10441 acgccgttat gaattaagtt ttataatgat ttctcatgac cctaaaattt gtcaagctat
    10501 ctgtaaccgg gtctttctga taaaaaatgg ttatttagta gaagataatg aattcttgaa
    10561 gagagcgtgt tctactaact gtttgaccaa tctctgaatt attatgtttt tcttatttta
    10621 ggagttgata aagaaatcaa cttactagtg tcattgctct attaaaattt tgccttttat
    10681 ggtaaaataa taaagacaaa ataaggagaa catagtagtg gaacctaaat atcaacgtat
    10741 attaattaag ttatctggtg aagcattggc gggagataag ggtgtcggta ttgacattcc
    10801 taccgttcaa tctattgcaa aagaaattgc cgaagtacac aattcaggtg ttcaaatagc
    10861 acttgtcatt ggtggaggta acctttggcg tggagaacct gcagcagaag cagggatgga
    10921 tcgtgttcaa gctgattata cagggatgtt agggacagta atgaatgctc ttgtaatggc
    10981 tgatagcctt caacaatatg gggtagatac tagagttcaa acagctattc caatgcaaac
    11041 tgttgctgag ccttacgtac gtggtcgtgc tttacgtcat ctagagaaga atcgtattgt
    11101 tgtctttgga gcgggtattg gttcgcctta tttctcaact gatacaacag cagcgcttcg
    11161 tgctgctgag attgaggcag aagcaatttt aatggctaaa aatggagtag atggtgttta
    11221 taatgctgat ccaaagaaag atgccaatgc tgtcaaattt gatgaattaa ctcacgttga
    11281 agtaataaaa cgagggttaa aaatcatgga tgcaaccgca tcaactattt caatggataa
    11341 tgatattgac cttgttgttt tcaatatgaa tgagactggt aatattaagc gtgttgttct
    11401 tggagaacaa atcggaacaa ctgtttcaaa caaagcatct gaataggagt gtataaaaaa
    11461 tatgactaag gaaatcgtta caaaagctca agagcgtttt gagcaatcac accaaagttt
    11521 atcaagagaa tttgcaggga ttcgtgcagg acgtgctaat gctagcttgc ttgatcgtat
    11581 tcaagttgag tattatggag cgccaacccc acttaaccaa ttagcctcta ttactgtacc
    11641 agaagctcgt gtccttttaa tttcaccatt tgataaatca tcaatcaaag atattgagcg
    11701 tgctattaac gaatcagatt taggtattaa tcctgcaaat gatggttcag ttattcgtct
    11761 tgttattcca gctttgacag aagaaacacg tcgtgattta gctaaagaag tgaaaaaagt
    11821 cggagaaaat gctaagattg ctatccgtaa tattcgccgt gatgctatgg atgaagctaa
    11881 gaagcaagaa aagaataaag aaattacaga agatgacttg aaatctcttg aaaaagatat
    11941 tcaaaaggct actgatgatg ctgtaaaaca tattgatgag atgactgcta ataaagaaaa
    12001 agaattgttg gaagtttaat agtaacgttt gctatcttta ttatgattgg caagtaataa
    12061 aatgattatg gaggtaactg atagaatcaa aaccagatgg atttggtttg tcggttacct
    12121 ttttaaaagg agaaaaatga atacattatt agcgacagtc ataacaggct tagtaactga
    12181 tgaaaacaaa gatttttatt ttatccaaaa agatggcttt acctttgcat tatcaaagtc
    12241 agaaggagag catcacattg gtgaaatggt taaaggattc gcctatacag atatgcaaca
    12301 aaaagcacgt cttacaacta aagaaacatt tgcgactcga gatcattatg gttgggggac
    12361 agttactgag gtacgtaaag acttgggagt atttcttgat actggtcttc cagataagca
    12421 agtggttgtt tctttggatg ttcttcctga attgaaagaa ctttggccta aaaaaggtga
    12481 tcgcctctac gtttgcttag atgttgataa aaaagatcgt ctctgggctc tccctgcaga
    12541 tcctgaagtg tttcaacgaa tggcaacccc tgcatataac aatatgcaaa atcaaaattg
    12601 gccagcaatc gtttatcgct taaaattgtc aggaactttt gtttatctac cagaaaataa
    12661 tatgctcggc tttattcacc ctagtgaacg ttacagtgaa cctcgtttag ggcaagtgct
    12721 ggatgcacga gttattggct tcagagaagt tgatagaacc ttaaatttat cattaaagcc
    12781 tcgttctttt gaaatgttag aaaatgacgc acaaatgatt ttaacgtatt tagaaagtaa
    12841 cggaggattc atgactttaa atgataaatc ttctccagag gaaattaaag ctacttttgg
    12901 aatttccaaa ggtcagttta aaaaagcact tggaggactc atgaaagcta aaaagatcaa
    12961 acaagatcag ctaggtacag aattattata aacttttgaa cttttaaagt tttctcacga
    13021 ggtcgtttta gttatagcta gaattaatgg ctaattatag aaaaaagctg agaggaaatt
    13081 ttggcttttt ttgttataat gtatctatta aaaagaatgg agaacgatat ggaacgtgcg
    13141 atttttgctg gaggttgttt ttggtgtatg gtacaaccat ttgaagaatt agatggtatt
    13201 gaatctgttt tatctggata cactggagga catgttgaga atccaactta taaagaagtt
    13261 tgtagtaaaa ctactggaca tacagaagct gttgaaatta tttttaatcc tgaaaaaatc
    13321 tcttatgcaa atttagttga gttatattgg gcacaaacag atccaacaga cgcttttggt
    13381 cagtttgaag atcgtgggga taattatcgt cctgtcatct tttatgagaa tgaagagcaa
    13441 cgtcagattg ctcaaaaatc aaaagataag ctacaagctt cgggtagatt tgatagacca
    13501 attgtaacca gcattgagcc tgctgatact ttttacccag cagaagacta tcatcaagca
    13561 ttctatcgga ctaaccctgc tcgttacgcc ttgagtagtg ccagaagaca tgcatttcta
    13621 gaggagaact ggcattgaga aaatcatttt acagttggtt gatgacacaa agaaatccaa
    13681 aatcaaatga accagtagca atcctagcag attacgcttt tgatgaaacc acttttccaa
    13741 aacatagttc ggattttgaa acagttagtc gttatttaga agatgaggcc agtttttcct
    13801 ttaatttaac agattttgat gatatttggg aagattatct caatcattaa caataatata
    13861 gagattgtat tttcaatctc tttttttagg ttatagaaat tggacacatt gtctaagact
    13921 gtctgaaaag caggtttttt aatggcaatc gttttcacaa aggagtagaa tatagtcatc
    13981 taaaatatag ccctagggag gttgtttatg agatatacaa atggaaattt tgaagccttt
    14041 gcaagacctc gaaaacctga aggtgtggat aaaaaatccg cttatattgt tggttctggt
    14101 ttagcaggat tagctgccgc tgtcttttta atacgtgatg gtcaaatgga tggtcaacgt
    14161 attcatattt ttgaagaact acctctttct ggaggatcac ttgacggtgt caaacgacct
    14221 gatatcggtt ttgtaacgcg tggtggtcgt gaaatggaaa atcacttcga atgtatgtgg
    14281 gatatgtacc gttccatccc ctctctcgaa gttccagatg cttcttatct agatgaattt
    14341 tattggcttg acaaggatga tcccaattca tctaactgtc gcctcattca taaacaggga
    14401 aatcgcttag aatctgatgg ggattttaca ctcggaacac attccaaaga gttagttaag
    14461 ctagtcatgg agactgaaga gtctttaggt gctaaaacga ttgaagaagt cttttcaaaa
    14521 gaattttttg aaagtaattt ttggacttat tgggctacta tgtttgcctt tgagaaatgg
    14581 cattcagcga ttgaaatgcg tcgatatgct atgcgcttta tccatcatat tggtggtctg
    14641 cctgatttca cttcattaaa atttaataaa tataatcaat atgattctat ggtgaaacca
    14701 atcattagtt atttggagtc tcataatgta gatgttcaat ttgatagcaa ggtaactaat
    14761 atctccgtag actttaagaa cggtcagaag cttgcaaaag ctattcatct cacagttggc
    14821 ggtgaagcaa aaacaattga tcttactcca gatgattttg tttttgtaac gaatggatct
    14881 atcactgaaa gtacgaacta tggtagtcat gatactgtag ctaaacctaa taccgatttg
    14941 ggaggttctt ggaatctttg ggaaaactta gctgctcaat ctgatgaatt tggacatcct
    15001 aaagtcttct acaaagatat tccaaaagag tcatggtttg tttctgctac tgcaacaata
    15061 aaagatccag ctatcgaacc ttatatagag cgtttaacac atcgtgacct ccatgatggt
    15121 aaagtaaata caggaggcat tgttacagtt acagattcta attggatgat gagctttgct
    15181 atccatcgcc aaccgcattt caaagagcaa aaggaaaatg aaacaattgt ttggatttat
    15241 ggactctatt caaatgtgga aggtaattat attaaaaaac caattgaaga atgtactggt
    15301 cgagaaatca cagaagagtg gttgtatcac ttaggtgttc ctgaaatgaa gattcatgat
    15361 ttatctgata aacaatatgt aagtacagtt cctgtttata tgccttacat tactagttat
    15421 ttcatgcctc gagttaaagg tgaccgacca gatgttatcc cgcaaggttc tgttaaccta
    15481 gccttcattg gtaattttgc agaatctcca tccagagata cagtatttac aacggaatat
    15541 tctattagaa ctgctatgga agctgtttat actttcttaa atattgaacg tggtgttcca
    15601 gaagtcttta attcagcatt tgatattcgt gtcttgttgc aatctttata ctatctcaat
    15661 gataagaaat ctgttgaaga tatggattta ccaatacctg ccttaatgcg taaagtaggc
    15721 atgaaaaaaa ttagaggaac ttatttagaa gaattacttc gagaagctca tcttttataa
    15781 tgtaaaataa aatcgagaaa acttgttttt ctcgattttt tagttttaca acgactaaaa
    15841 tcaccaaaga aaatgaaatt ttgttataat gtagtcactt actgtacgaa aaggaagaat
    15901 aatttgcaag aatactctat cgaaattact ttacaacatc cagatgatat gatgtcactc
    15961 tttggcagca atgagcgcca tttaaaatta attgaagaaa atttagatgt tatcattcat
    16021 gctagaactg aacgtgttca ggtattaggt gactctgaag aagcagtaga aaccgctcgg
    16081 ttaaccattg aagcattatt agtgcttgtt aaccgcggga tgactgtgaa tacttcagat
    16141 gttgtaacag ccttatccat ggcacaaaat ggttcaattg ataaattcgt tgccctttat
    16201 gaggaagaaa ttattaagga tagctatggt aagcctatcc gtgttaagac tttagggcaa
    16261 aaaatatacg ttgatagcgt taaaaatcat gatgttgttt ttggtattgg gcctgctgga
    16321 acgggaaaaa cttttttagc agtcactcta gcagtaacgg cactaaagcg tggtcaagtt
    16381 aaacgaataa tcttaacgag accagcggtt gaagctggtg aaagccttgg tttcttgcct
    16441 ggagatctca aagagaaagt tgatccttat ttgagaccag tttatgatgc cctatatcaa
    16501 attttaggta aagagcaaac aagtcgctta atggagcgtg aaatcatcga aattgctcca
    16561 ttggcttaca tgcgtggtcg tactttagat gatgcatttg tcatattaga tgaagcgcaa
    16621 aacaccacta taatgcaaat gaaaatgttc ctaacacgtc ttggcttcaa ttctaaaatg
    16681 attgtaaatg gcgatgttag ccaaattgac ttaccaaaga atgttaaatc tggtttgatt
    16741 gatgctgttg agaaattacg aaatatcaaa aagattgact ttattcattt atcagcaaag
    16801 gatgttgtaa gacaccctgt cgttgctgaa ataataaatg cttattcaga ttcagaaagt
    16861 agtcacaagc tacaatctga taaggactga tgtttgccac taggagacgt tgttagagag
    16921 tgtgtcaata tgtaaaaaac gcctaatgct ttaggcgttt ttatgatggt gtacttcaac
    16981 ttaacctatc aagtgttgaa aattttcttt aagcatgacg tcgagatgca ttattatatc
    17041 gtttaatgtt tctttaacag tgtgttcgtt ggagttgtga tgacatagtt taatgatatt
    17101 ttcttcgaaa tgtttaggaa gcaatgtagc attcttctta gcataggaaa tcattctttt
    17161 ttcgccagga tgtgtcaact tattaagtgc aaaaataata tcaaagtagg aatctaagaa
    17221 agcagtagtt ctatggtgag tactaacaaa gtcttggcgt ttaagggctt ttataatttg
    17281 tttatcgtaa gagggaagct tgccagttaa taagttacga ttttgaatga taatttgttt
    17341 ttgtaactga ctgggataag aaacttcgaa tctctctttg agtgattgta attgattttc
    17401 aggatcatag agtatttggc aattgatgag gttatgccaa aaacaagtag tgtatccaat
    17461 tcgagaattg tggtgttcaa cgacatcttc taagtctgat aaaaagttat ctatgttacg
    17521 atagagaata tcaatatcag ttccgtcgtt taaagtacca ttatcttcaa gctcccagta
    17581 atgattgtta agttcaatgt aatgacagtg cttgttaagg atacgtttac gactagtgat
    17641 atcaggagtt gctgcacaat aaacatacaa atcataatcg gaagattggt caaaactgtc
    17701 accagagcgt gagcctccga gtgctatagc aacaacattt ggaagtttag agaaatcttg
    17761 tcagagtttt gtgaaatggt ccataaatgc tctcctaaaa atgtaaaata ttgatgtgtc
    17821 tccattctac agggctatct ctttttagtc aataattaat agtaagttaa taaaagaggt
    17881 aacggttcta atattcaaga agtgtatatg agtgatttga aaaggagata ttttatgcta
    17941 caatacgtac atattagttt aaacaaggtg atagaatgaa acaagattat atttcctaca
    18001 ttaggtcaaa agttggacat gagactatct ttttaacata ttctggtggt attttgacag
    18061 atggtaaggg acgtgtctta ctccaattaa gagctgataa gaattcttgg ggaattattg
    18121 gtggatgtat ggagttaggt gagtcatcag tagatacatt gaaaagagaa tttttcgaag
    18181 aaactggatt gagagtggaa ccaatcagac tgttaaatgt ttatactaac tttcaagact
    18241 cttatcccaa tggcgataaa gcacaaaccg tcggttttat ttatgaagta agctgtccaa
    18301 aaccagttaa tatagaaggt tttcataatg aggaaacatt gcaattggac tatttttcta
    18361 aagaagacgt caaaaatatt acgatcgtaa atgaacaaca ccaattaatt ttagatgaat
    18421 atttttcaca aacattccaa atggggcgtt aaaaggatgg gaaatactag gtgtgacttt
    18481 aaagaacttc taacttttat tgatatgttg ttaggcattc ttgtagaaac aaatgttaag
    18541 aaaataagga gttctcattt gtatatttta taattattaa aaagaaaaac agtagcttgc
    18601 aataatttag tgactgtttt tttagaaagc ataagaagta cagaaaaaga aaattatgga
    18661 atattggatg gtggggatat gctttactta cggtattgga atgatatact taaagaaatc
    18721 cagttttaga aatctaaatt tctaagtaaa atagcataaa ggagagtaga tgaatgaaga
    18781 taacattaca cggcgttgct gaaacgttat taatcacctt atatattagg gctaaagatg
    18841 cgatggctaa acatccgata cttaatgatc aaaaatcctt agcaattgtt gaacagatag
    18901 aatatgattt tgataaattc gataattcag aagcttcttt ttatgcaaca ttagctagaa
    18961 ttcgcgttat ggatagagaa atcaaaaaat ttattagaga aaatccaaat agtcaaatcc
    19021 tttcaattgg ttgtggactt gatacaaggt ttgaaagagt cgataatgga caaattaggt
    19081 ggtataacct tgatttgcca gaggttatgg agataagaaa attatttttt gaagagcatg
    19141 aaagagttac taatatagca aaatcagccc tagatgaaac ttggacacgg gaggtaaatc
    19201 cccaaaatgc cccttttcta atcgtgtcag aaggtgtttt aatgtttcta aaagaagatg
    19261 acgtagagac ttttcttcat atcctgacaa attcatttag ccaatttatg gcacaatttg
    19321 atttgtgtca taaggaaatg attaataaag gaaagcaaca tgatacagta aagtatatgg
    19381 atacagaatt tcagtttggt atcacagatg gtcatgagat tgtggattta gaccctaaat
    19441 taaagcaaat aaatctgatt aactttacag atgagatgag caaatttgag ttaggcacac
    19501 ttcgctcttt acttccaaca attcgtaaat ttaat
//
LOCUS       DATRTN010000027        19289 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.027, whole genome shotgun sequence.
ACCESSION   DATRTN010000027 DATRTN010000000
VERSION     DATRTN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19289
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.027"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            221..1117
                     /locus_tag="VBE22_000998"
     CDS             221..1117
                     /locus_tag="VBE22_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7202468.1"
                     /translation="MFNKIGFRTWKSGKLWLYMGVLGSTIILGSSPVSAMDSVGNQSQ
                     GNVLERRQRDADNKSQGNVLERRQRDVENKSQGNVLERRQRDVENKSQGNVLERRQRD
                     ADNRSQGNVLERRQRDVENKSQGNVLERRQRDVENKSQGNVLERRQRDVENKSQGNVL
                     ERRQRDAENKSQGNVLERRQRDVENKSQGNVLERRQRDVENKSQVGQLIEKNPLLSKS
                     TVSRENNHSSQGDSNKQSFSKKVSQVTNVANRPMLTNNSRTIPVINKLPKTGDDQNVI
                     FKLVGFGLILLTSLCGLRRNEN"
     gene            1325..2401
                     /locus_tag="VBE22_000999"
     CDS             1325..2401
                     /locus_tag="VBE22_000999"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEO7202469.1"
                     /translation="MGYTVAIVGATGAVGTQMIRQLEQSNLPIEQVKLLSSSRSAGKI
                     LHFKDEAIRVEETTKKSFYDVDIALFSAGGSISAKFAPYAVKSGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHAMIGHNGIIACPNCSTIQMMIALEPIRQKWGIERVIVSTYQAVSGS
                     GARAVEETKEQLRQVLNDNLSPDQLIATVLPCSSDQKHYPIAFNALPQIDIFTDNDYT
                     YEEMKMTLETKKIMEDATIKVSATCVRIPVLSGHSESIYIETKELASISEIKKAIANF
                     PGAVLQDLPSQQIYPQAINAVGHRETFVGRIRKDLDQENGVHMWVVSDNLLKGAAWNS
                     VQIAETLHKNGLVKPAKELKFELI"
     gene            2571..2909
                     /locus_tag="VBE22_001000"
     CDS             2571..2909
                     /locus_tag="VBE22_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202470.1"
                     /translation="MQIIKRDGQIVEFDPEKIYQAILKAAQTVYVLDESLRQDLAQVT
                     KKVILDLEDAQVERPTINMIQSLVENRLLDAGYIHIAEHYISYRLQRDLERNGYDDQI
                     TVHLHFEKIK"
     gene            3040..4581
                     /locus_tag="VBE22_001001"
     CDS             3040..4581
                     /locus_tag="VBE22_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO7202471.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLIGANGEGKST
                     FMSIVTGHLQPDEGKIEWSKYVTAGYLDQHTVLEEGQTVRDVLRTAFDELFKTEARIN
                     DIYISMADDGADVDSLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDSDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQDLVRYSGDYTNFESVYAMKKAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPSFEFKESRTPGRFIFQAKDLQIGY
                     DRALTKPLNLTFERNQKIAIVGANGIGKTTLLKSLLGIIPPISGNVERGDFIDLGYFE
                     QEVPGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQSKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKAYKGSILMVCHEPDFYEGWMDDVWD
                     FNQLS"
     gene            4621..5853
                     /locus_tag="VBE22_001002"
     CDS             4621..5853
                     /locus_tag="VBE22_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEO7202472.1"
                     /translation="MQNDMSVVLRAGKLLIESGAEVYRVEDTMKHFAKALQIENFEAY
                     VVSSSIIASGINRYGKQEAKVCNTDGVTANLGRLEAVNNLSRQIAKQDLVSPEEIVKQ
                     LDLIEHQKDYSLLVTLISYFCGAGSFSLALGSSLLDSFSAAVTGLILGYFLNLMESRI
                     HTGFLLTILGSSVVALSANLLYFSGLGEHRSIIILGALMVMVPGAAFVNSVREFSQNN
                     FSTGLALIMSALLICISISAGVAITIEIIPFAEQMTGSFSGVPNTILEIIIRTLMAGL
                     GTIAFSILYHVPKRYFLDLGILGAISWMLYLILWQQFHMDAIAVFFPGLFITYFSRLL
                     AAKRKCPATVFLATSMFPLIPGLSFYRAVYFLLTGADALAMEYFRSCFVTAFTIAIAI
                     SIVQQIPLSFFIRRKMIK"
     gene            6066..6767
                     /gene="pyrF"
                     /locus_tag="VBE22_001003"
     CDS             6066..6767
                     /gene="pyrF"
                     /locus_tag="VBE22_001003"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEO7202473.1"
                     /translation="MLEKCPIIALDFSDLASVTTFLEHFPKEELLFVKIGMELYYSEG
                     PSIIRYIKSLGHRIFLDLKLHDIPNTVRSSMSVLAKLDIDMTNVHAAGGVEMMKAARE
                     GLGEGPILLAVTQLTSTSQEQMQVDQHINLSVVDSVCHYAQKAQEAGLDGVVASAQEV
                     KQIKKQTNEHFICLTPGIRPPQTNQLDDQKRTMTPEQARIVGADYIVVGRPITKAENP
                     YQAYLDIKEEWNRIK"
     gene            6780..7409
                     /locus_tag="VBE22_001004"
     CDS             6780..7409
                     /locus_tag="VBE22_001004"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEO7202474.1"
                     /translation="MDLARQIAMELLDIQAVYLRPQQPFTWASGVKSPIYTDNRVTLS
                     YPETRTLIENGFVKQIQKHFPNVDIIAGTATAGIPHGAIIADKMNLPFAYIRSKAKDH
                     GVGNQIEGRVYSGQKMVIIEDLISTGGSVLEAVTAAQSQGIEVLGVVVIFTYQLAKAE
                     QAFREADIPLVTLTDYNQLIKVAKVNGYITADQLVLLKKFKEDQMNWQS"
     gene            7421..8713
                     /locus_tag="VBE22_001005"
     CDS             7421..8713
                     /locus_tag="VBE22_001005"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEO7202475.1"
                     /translation="MYIIKNGLIIDPQSGFNQVSDMLIDQGKIKQISKEIDIKGIPII
                     DASNKIVAPGLVDIHVHFREPGQTHKENIHTGALSAAAGGFTTVLMMANTNPTISSLE
                     IVKQVKESAAKEAIKIETVATITKSLNGKDLVNFEELLEAGVAGFSDDGIPLTDTKVL
                     QEAMNLARKHDVVLSLHEEDPSLNGVLGINEHIAQKIYHVCGASGLAEYSMIARDAMI
                     AYQTQAKVHIQHLSSSESVEVVDFAQKLGANLTAEVTPQHFSKTEDLLLTKGANAKLN
                     PPLRLEKDRQALIDGLKSGVISIIASDHAPHHIMEKAADNISQVPSGMTGLETSLALG
                     ITYLVSTKELSMIDFLAKMTCNPAQLYGFDAGYLREGGPADIVIFDQAEERIIKAEFA
                     SKSSNSPFIGDKLKGVVHYTICNGEIVYQKDSHAATLV"
     gene            8878..9804
                     /locus_tag="VBE22_001006"
     CDS             8878..9804
                     /locus_tag="VBE22_001006"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEO7202476.1"
                     /translation="MNHQTQTLSLEHFVSLEELSNQEVMSLIKRSIEVKENPSNIGFD
                     KDYYVSNLFFENSTRTHKSFEMAELKLGLKTIEFNADTSSVNKGETLYDTILTMSALG
                     LDVCVIRHPDIDYYKELIASPNIHSAIVNGGDGSGQHPSQSLLDLVTIYEEFGYFKGL
                     KIAIVGDLTHSRVAKSNMQVLKRLGAEIFFSGPKEWYSSQFDEYGQYLPIDQLVDQID
                     VLMLLRVQHERHDGKGVFSKESYHQQFGLTKERYKHLRDTAIIMHPAPVNRDVEIASD
                     LVEADKARIVKQMSNGVYARIAILEAVLNSRQ"
     gene            9815..10891
                     /gene="carA"
                     /locus_tag="VBE22_001007"
     CDS             9815..10891
                     /gene="carA"
                     /locus_tag="VBE22_001007"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEO7202477.1"
                     /translation="MKRLLLLEDGSVFEGEAFGADVETSGEIVFSTGMTGYQESITDQ
                     SYNGQIITFTYPLIGNYGINRDDYESIRPTCKGVVVYEWAEYPSNWRQQMTLDEFLKL
                     KGIPGISGIDTRALTKIIRKHGTMKACLINEGNSIHEALENLQESVLLNNQIEQVSTK
                     LAYASPGVGKNIVLVDFGLKHSILRELSQRQCHITVIPHTTTAQEILNLNPDGVLLSN
                     GPGNPEQLPNALQMIQEIQSKIPIFGICMGHQLFAKANGAKTYKMTFGHRGFNHAVRH
                     LQTGQVDFTSQNHGYAVSREDFPEALFITHEEINDKTVEGVRHKYYPAFSVQFHPDAA
                     PGPHDTSYLFDEFINMIDDFQQKS"
     gene            10922..14104
                     /gene="carB"
                     /locus_tag="VBE22_001008"
     CDS             10922..14104
                     /gene="carB"
                     /locus_tag="VBE22_001008"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEO7202478.1"
                     /translation="MPKRTDIRKIMVIGSGPIVIGQAAEFDYSGTQACLSLKEEGYQV
                     VLVNSNPATIMTDKDIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMAL
                     SKNGILEELNVELLGTKLSAIDKAEDRDLFKQLMEELNQPIPESEIVNSVEEAIQFAE
                     QIGYPLIVRPAFTLGGTGGGMCDNQEQLVDITTKGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPAQTLSDVENQLLRDASLDIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPITKTTYAMFEPALDYVVAKMPRFPFDKFESGDRKLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVDHIKIADLDNVSDDVLLEKIRKAEDDRLFYLAEALRRHYSIEKLAS
                     LTSIDSFFLDKLRVIVELEDLLSKNRLDINILKKVKNKGFSDKAIASLWQINEDQVRN
                     MRKEAGILPVYKMVDTCASEFDSATPYFYSTYAVENESLISDKASILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIRESGFEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIDL
                     EKPEGVILQFGGQTAINLAKDLNKAGVKILGTQLEDLDRAENRKQFEATLQALNIPQP
                     PGFTATTEEEAVNAAQKIGYPVLVRPSYVLGGRAMKIVENEEDLRHYMTTAVKASPDH
                     PVLIDAYLIGKECEVDAISDGQNILIPGIMEHIERAGVHSGDSMAVYPPQTLSETIIE
                     TIVDYTKRLAIGLNCIGMMNIQFVIKDQKVYVIEVNPRASRTLPFLSKVTHIPMAQVA
                     TKVILGDKLCNFTYGYDLYPASDMVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     INLQKALYKAFEAAYLHMPDYGNIVFTVDDTDKEEALELAKVYQSIGYRIYATQGTAI
                     YFDANGLETVLVGKLGENDRNHIPDLIKNGKIQAVINTVGQNNIDNHDALIIRRSAIE
                     QGVPLFTSLDTAHAMFKVLESRAFTLKVLD"
     gene            14231..14554
                     /locus_tag="VBE22_001009"
     CDS             14231..14554
                     /locus_tag="VBE22_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202479.1"
                     /translation="MLKTRTHINELETKIALDLVHRYLGIQQLVFSKWRHSLETGSWS
                     SIITINGVNIINYIVDDISDSNSISGIICHPKSFHLRPREKKEPIQSIDELDITWSLN
                     REENE"
     gene            14551..15333
                     /locus_tag="VBE22_001010"
     CDS             14551..15333
                     /locus_tag="VBE22_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HEO7202480.1"
                     /translation="MIPDSSFKVLSDMAYNIEKSKAESNGHLQIIEGSQIIDFHQKRS
                     FMVLEVNDDINNGMQAMACQSLDESNHIVIAYSGTNAFNKKDILTDIDSVVLGKNNKL
                     LTCPSSITLREDGISLPCKNPSQITTSKLFAQKIKMNYPDATLWLTGHSLGGYLAMIV
                     ASEMRLSARVFNSPSPINNMSEAATSYVISHPEKYHSYRINKDYIGNFGINSSDDKLG
                     ISKRVNAKKQHGFYKKIFFHELTRFRLNQQGQIVDIDENKQD"
     gene            15450..15740
                     /locus_tag="VBE22_001011"
     CDS             15450..15740
                     /locus_tag="VBE22_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEO7202481.1"
                     /translation="MAQIKLTPEELRSSAQKYTAGSQQVTEVLNLLTQEQAVIDENWD
                     GSTFDSFEAQFNELSPKITEFAQLLEDINQQLLKVADIIEQTDADIASQISG"
     gene            15822..18832
                     /gene="esaA"
                     /locus_tag="VBE22_001012"
                     /pseudo
     CDS             15822..18832
                     /gene="esaA"
                     /locus_tag="VBE22_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
     gene            18841..>19289
                     /gene="essA"
                     /locus_tag="VBE22_001013"
     CDS             18841..>19289
                     /gene="essA"
                     /locus_tag="VBE22_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HEO7202482.1"
                     /translation="MKLKRFLACLLILSCMSQTSVFSDGLKNNDIHFDTKRLESDSQK
                     NSGFQTELTKDLFKKEDLKKLDKNRQSKVSLQKKQSKQLFQYSNKGQSDRKKRIKQLF
                     SEESNQFKFVTRTEEPTVSADIGHIIIYLVLISLVLILASIMTFKIK"
BASE COUNT         6611 a         3051 c         3687 g         5940 t
ORIGIN      
        1 gtatgactat ctatcatgtt ttataatatt gaaggaaaat ttaaaaatat catgttttag
       61 atatcaacta tttaatttta aacatacaaa ttaataataa attgcaatta aataacaaat
      121 taccttgaca taaattataa aatgttttaa tatatataat ctaaataaaa ataataataa
      181 aatgactttt aaaatttaaa aaaagtaagg agaaaattaa ttgttcaata aaataggttt
      241 tagaacttgg aaatcaggaa agctttggct ttatatggga gtgctaggat caactattat
      301 tttaggatca agtcctgtat ctgctatgga tagtgttgga aatcaaagtc agggtaatgt
      361 tttagagcgt cgtcaacgtg atgcggataa caagagccag ggcaatgttt tagagcgtcg
      421 ccaacgtgac gttgagaata agagccaagg caatgtttta gagcgtcgcc aacgtgacgt
      481 tgagaataag agccaaggca atgttttaga gcgtcgtcaa cgtgatgcgg ataacaggag
      541 ccagggcaat gttttagagc gtcgccaacg tgacgttgag aataagagcc aaggcaatgt
      601 tttagagcgc cgccaacgcg atgttgagaa caagagccaa ggcaatgttt tagagcgtcg
      661 tcaacgtgat gttgagaata agagccaagg caatgtttta gagcgtcgtc aacgtgatgc
      721 agaaaacaaa agtcagggca atgttctaga gcgtcgtcaa cgcgatgttg agaataagag
      781 ccaaggcaat gttttagagc gccgccaacg cgatgttgag aacaagagcc aagtaggtca
      841 acttatagag aaaaatccac ttctttcaaa gtcaactgta tctagagaaa ataatcactc
      901 tagtcaaggt gactctaaca aacagtcatt ctctaaaaaa gtatctcagg ttactaatgt
      961 agctaataga ccgatgttaa ctaataattc tagaacaatt ccagtgataa ataaattacc
     1021 taaaacaggt gatgatcaaa atgtcatttt taaacttgta gggtttggtt taattttgtt
     1081 aacaagtctc tgcggtttga gacgcaatga aaattaagta taatcaacca tttagtaact
     1141 attataatga tatatgcaat caataaaaag gaatcggata cgagattcct ttttataatt
     1201 aggttggtta gggtgacttt tttcatttgg ctattcttga aagtttataa aaatgtagta
     1261 taatagtcac attaaaatgt tttgaaaata ttgatgaaca acatcaacaa atagaggtca
     1321 ttatatggga tataccgttg ctatcgtagg tgctacaggt gccgtaggaa cacaaatgat
     1381 tcgtcaatta gaacaatcga atttaccaat agaacaagtg aaacttttat catcaagtcg
     1441 ctcagcaggt aagattttac attttaaaga tgaggctata cgtgttgaag agacaactaa
     1501 aaaatcattt tacgatgttg atattgcctt gttttcagct ggtggatcta tttcagcaaa
     1561 gttcgctcct tatgcagtaa agtctggagc agttgtagta gataacacgt catattttcg
     1621 tcagaatcct gatgttccac tagttgttcc tgaagtaaat gctcatgcca tgattggtca
     1681 taatggtatc atagcttgcc ccaattgttc tactattcaa atgatgattg ctttagagcc
     1741 cattcgtcaa aaatggggga tagagcgtgt tatagtttcc acctatcaag ctgtttcggg
     1801 ttcaggtgca cgtgctgttg aagaaactaa ggaacagttg agacaagttt taaatgataa
     1861 cctttcacct gatcaattaa ttgcaacggt cttgccatgt tctagcgatc aaaaacatta
     1921 tcctatcgca ttcaatgcat tacctcaaat tgatattttt actgataatg attatacgta
     1981 cgaagaaatg aaaatgactt tggagacaaa gaaaattatg gaagatgcta ctattaaggt
     2041 ttcagctacc tgcgtccgca ttcccgtttt atcgggtcat tctgagtcta tttatattga
     2101 gacgaaagaa ttagcctcaa tctctgaaat taaaaaggct attgcaaatt ttcctggtgc
     2161 cgtcctacaa gaccttccat ctcagcaaat ttaccctcaa gcgattaatg ctgtaggcca
     2221 tagggagacc tttgttggtc gtattcgaaa agaccttgat caagaaaatg gtgttcatat
     2281 gtgggtagtt tcagataatc tactgaaggg ggcagcttgg aattctgttc aaatcgcaga
     2341 aacattgcat aagaatgggc tggttaaacc agcgaaagag ttaaaatttg aattaatata
     2401 gaagaatatg acattgctta atacttatac tgacttctag tgatattaat ttttcatcaa
     2461 tatcaggtaa aacacttgac aaattatgaa aacatttgca taatgaaatt aacaaatgaa
     2521 aatgattgca aatcgtttta acattgtttc cacattaagg aggggctctt atgcaaatca
     2581 tcaaacgtga tggacagatt gttgaatttg atcctgaaaa aatctaccaa gctatcttga
     2641 aagctgctca aacagtttat gtgcttgatg agtcacttcg tcaagacctt gctcaagtga
     2701 ctaaaaaagt aatcttagat ttagaggatg ctcaggttga acgaccaacg atcaatatga
     2761 ttcagtcttt ggttgaaaat cgtttattgg atgcaggata cattcatatt gctgaacact
     2821 atatttcata ccgtttgcaa cgtgatttag agcgaaatgg ttatgatgat caaatcactg
     2881 ttcatttaca ctttgaaaaa attaagtaag gtttgacttt taagtttaaa ctctctatac
     2941 ttgtatatgg aacttactat gtaatgtgct aatttaaaaa cttgtagagt gtctctatga
     3001 gttttttaat aacctctatt atcaatcaaa ggattttaaa tgagtatttt agaagtaaaa
     3061 aacttaagtc acggttttgg tgatcgtgct atttttgaga atgtttcttt tcgcctttta
     3121 aaaggtgaac atataggact tattggtgct aatggtgaag gaaaatcaac attcatgtca
     3181 attgttacag gtcatcttca acctgatgaa ggaaaaattg aatggtcaaa atatgtaact
     3241 gctggttacc ttgatcaaca tactgtgtta gaagaaggtc aaacagttcg tgatgttttg
     3301 cgaacagcat ttgatgaact ttttaaaaca gaggcacgta tcaatgatat atatatatcc
     3361 atggcagatg atggcgcaga tgtagatagc cttatggaag aagttggaga actacaagat
     3421 cgtttagaat cacgtgattt ttatacgctt gatgctaaaa ttgatgaagt cgcacgggca
     3481 ttaggagtta tggattttgg tatggattct gatgttactg aattatctgg tggtcaaagg
     3541 acaaaggttc ttttagcaaa actgttatta gaaaagccag atatcctcct gcttgatgag
     3601 cctacaaact accttgatgc tgaacatatt gattggttaa aacgctattt acaaaattat
     3661 gaaaatgctt ttgttttaat ctcacacgat attccatttt tgaacgatgt tatcaatatt
     3721 gtctatcatg ttgaaaatca agatttagta agatattccg gtgattatac taattttgaa
     3781 tcagtctatg caatgaaaaa ggctcaatta gaagcagcct atgaacgtca acaaaaggaa
     3841 atagctgatt tacaagattt tgttaatcgt aataaagcac gcgttgcaac acgtaatatg
     3901 gcaatgtctc gtcaaaagaa actagacaaa atggatatta ttgagttaca agcagagaaa
     3961 ccaaagccaa gttttgaatt taaagaatct cgcacaccag gacgttttat tttccaagct
     4021 aaagatcttc aaattggata tgatcgtgct ttaacaaaac ctctaaacct aacatttgaa
     4081 cgtaatcaaa aaatagctat tgttggagca aatgggatag gaaaaacaac attacttaag
     4141 agtttactag gtattatccc ccctatttct ggtaatgttg aacgaggtga ttttattgat
     4201 ttaggttatt ttgaacaaga agtacccgga gggaatcgcc aaactccact tgaagcagta
     4261 tgggatgctt ttcctgcttt gaaccaagca gaggttaggg cagctcttgc tcgttgtggt
     4321 ttaacttcaa agcatattga aagtcagatt caggtgttat ctggtgggga gcaatcaaag
     4381 gtcagatttt gcttattaat gaatcgcgaa aataatgtcc ttgtacttga tgaaccaacc
     4441 aatcatttag atgtagatgc taaagatgag ttaaaacgtg ctttaaaagc ttataaggga
     4501 tcaatcttga tggtatgtca tgaaccggac ttttatgaag gatggatgga tgatgtttgg
     4561 gactttaacc aacttagtta agtggctagg gtaataaagt agaaggcagg gccaaataag
     4621 atgcaaaatg atatgagcgt tgttcttaga gctggtaaat tactgattga aagcggagca
     4681 gaagtctatc gtgtcgaaga cacaatgaag cattttgcta aggctcttca gatagaaaat
     4741 tttgaagctt atgtggttag tagcagtatt atcgcttccg ggattaaccg ctatggtaaa
     4801 caagaagcta aagtatgcaa tacagatggt gtaacagcaa atttaggtag acttgaagct
     4861 gttaataacc tttctaggca aattgctaaa caagacttag tatctccaga agaaattgta
     4921 aagcagttag acttaattga acatcaaaaa gattatagtt tgcttgttac tttaatttct
     4981 tatttttgtg gagcgggtag tttttcttta gccttgggta gttccttatt agattcattt
     5041 tccgctgctg taacaggctt aattttagga tattttttga atttgatgga aagtcgtata
     5101 catacaggat ttcttttgac cattctaggt agttctgtag tggctttaag tgctaattta
     5161 ttatattttt caggattagg tgagcatcgt agcattatca tattaggggc actaatggtt
     5221 atggtgccag gggctgcctt tgttaattcg gtgagagaat tttcacagaa taatttttca
     5281 acaggtttag ctttgattat gtctgcttta ctcatttgta tttctatatc agcaggcgtg
     5341 gctattacaa ttgaaattat tccgtttgca gaacaaatga caggttcttt ttctggtgtt
     5401 cctaatacca tccttgagat tatcatcaga acactaatgg ccggtttagg gacgatagcc
     5461 ttctctattc tctaccatgt tccaaaacgt tatttccttg acttaggtat actaggagca
     5521 atatcttgga tgctctacct gatcttatgg caacaatttc atatggatgc tatagccgta
     5581 ttttttccag ggcttttcat cacttatttt tcacggctat tagctgctaa acgtaaatgt
     5641 cctgccactg tctttttagc gacaagtatg tttcctctta ttccaggact tagcttctat
     5701 agagctgttt actttttatt gacgggagca gatgctttag caatggaata ttttagatcc
     5761 tgttttgtaa cagcttttac aattgctata gcaatcagta ttgtacagca gattcctcta
     5821 tcgttcttta ttagacgtaa aatgataaaa taatttagta tttatttgac aaaatgattg
     5881 taaacttgta gaatacctac aaatgtatct ttaagtgagt ccagagaggc gaacaaggtc
     5941 accatcataa gtcaaatagc gagtgctttt tcgctaactt tttatgaaac cttgccctca
     6001 ggcaaggttt tttgtgtcat actggtacat ttccaacaat tattatttct atagagagga
     6061 attatatgtt agaaaaatgc cctatcattg ccttagattt ttctgatttg gcttctgtaa
     6121 caactttttt agaacatttt ccaaaagaag aattactttt tgtcaaaata ggaatggagc
     6181 tttattattc cgaaggtccc tcaattatta gatatattaa atccttagga catcgcattt
     6241 ttttggattt aaaattacat gatatcccta atacagttcg atcatctatg tctgtcttag
     6301 ccaaattaga cattgatatg actaatgtcc atgctgcagg aggagtcgaa atgatgaaag
     6361 cagctagaga aggtttgggg gagggtccaa tactattagc agtgacacaa ttaacctcga
     6421 cttcacaaga acaaatgcag gtggatcaac atattaactt aagtgtagtt gattcggttt
     6481 gccattatgc tcaaaaagct caagaagcag gcctagatgg agtagtcgca tcggctcaag
     6541 aggtaaagca aatcaagaaa cagactaacg aacatttcat ttgcttaacg cctggtatca
     6601 gacctccaca aaccaatcag ttagatgatc aaaaaagaac gatgactcct gagcaagcac
     6661 gtatagttgg ggctgattat atcgttgtgg gaagacctat aacgaaagca gaaaatccat
     6721 atcaagcata tctagacata aaagaagaat ggaatcgtat taaataaagg agtactgaga
     6781 tggatttagc aagacagatt gccatggaac tattagatat tcaagctgtt tacttaaggc
     6841 cccaacaacc ctttacttgg gcttcgggag tcaaatcgcc aatttacact gataatcgtg
     6901 taaccttatc gtatcctgag acaaggacac tgattgaaaa tggttttgtt aaacaaatac
     6961 agaaacattt cccaaacgtt gatatcatcg cagggacagc gactgcaggc ataccacatg
     7021 gcgctattat agctgataag atgaatttgc catttgccta tatacgctcg aaggcaaagg
     7081 atcatggtgt ggggaatcaa atcgagggac gtgtttactc aggccaaaaa atggttatca
     7141 ttgaagattt gatttcaaca ggaggatctg ttttagaggc tgttacggct gcgcagagtc
     7201 aaggtattga ggttttaggg gtagtagtga tatttacata tcaattagct aaggctgaac
     7261 aagcatttcg tgaagctgat attcctttgg tgactttgac ggattacaat caattaatta
     7321 aagttgctaa agttaatgga tatattacag ctgaccaact ggtattactg aaaaaattta
     7381 aagaagatca aatgaattgg caaagttaga gaggacagat atgtatatca ttaaaaacgg
     7441 attgattatc gaccctcaaa gtggttttaa tcaagtatca gacatgctga ttgatcaagg
     7501 aaaaattaaa caaatatcga aagaaattga tattaaaggc attccaatca ttgatgcaag
     7561 caataaaatt gtagctccag gattagtcga tattcatgta cattttcgtg agcctggaca
     7621 gacacataaa gaaaatatcc atacaggggc cctatcagct gcagcaggtg gttttacgac
     7681 agttcttatg atggccaata ctaacccaac gatttctagc ctagaaattg taaaacaagt
     7741 taaggagagt gcagcaaaag aagcaattaa gattgaaaca gttgctacaa ttactaaatc
     7801 cctgaatggt aaagatttag ttaatttcga agaactatta gaagctggag ttgctggatt
     7861 ctcagacgat ggtattcctt tgacagatac taaggttttg caagaagcta tgaacttagc
     7921 tagaaaacat gatgttgtcc tatcgttaca tgaagaggat ccatcgttaa atggcgttct
     7981 tggtatcaac gaacatattg ctcagaaaat ttatcatgtc tgtggagcta gtggtcttgc
     8041 tgaatattca atgattgcgc gagatgctat gattgcttac caaacacagg cgaaagtcca
     8101 tatccagcat ttgtcaagtt cagaatcagt agaagtagtt gattttgcgc agaagcttgg
     8161 tgctaactta acagcagaag taacccccca acatttttca aagacagagg acttactttt
     8221 aactaagggt gctaatgcaa aattaaaccc accactgcga cttgaaaagg atagacaagc
     8281 cctcattgat ggtcttaaaa gtggtgttat ttcaattatt gcaagtgatc acgctcccca
     8341 tcatattatg gagaaagcag cagataatat tagtcaagtg ccctctggta tgactggctt
     8401 agagacatca ttagccttag ggattactta tttagtatct actaaagaac tctcaatgat
     8461 tgactttttg gcaaaaatga catgtaatcc tgcacagtta tatggatttg atgcaggtta
     8521 tctcagagaa ggtggacctg cagatattgt tatttttgat caagctgaag agagaataat
     8581 aaaggcagaa tttgcctcca aatccagcaa ctctcctttt atcggtgata aattgaaggg
     8641 agttgttcac tacacaattt gcaatggtga aatagtctat caaaaagata gccacgcagc
     8701 aacattggta taggtaaaac atttcttatt aatatccttt aaggtattgt ccagagaggc
     8761 aaacaaggaa ttgcgttaac aaatagtaag ttagttctct atctagctta ctattttgca
     8821 gagcctcctt gttttaggag gcttttttct agctaaaatt tatagaaaga aattatcatg
     8881 aatcatcaaa cacaaacact ttcattagaa cattttgtca gtttagaaga attatcaaat
     8941 caagaagtta tgtctttgat aaagagaagt atcgaagtca aagaaaatcc tagcaacatt
     9001 ggttttgata aagattacta tgtgtcaaat ctcttttttg agaattcaac aagaacacat
     9061 aagtcatttg aaatggcaga actcaaatta ggattaaaaa caattgaatt taatgctgat
     9121 acaagttctg ttaataaagg agaaaccctt tatgacacta tcttaacaat gagtgctcta
     9181 ggattggacg tttgtgtcat taggcatcct gatattgact attataaaga attgattgct
     9241 agtccgaaca ttcattcagc tattgtcaat gggggtgatg gctcaggcca acatccaagt
     9301 caatcacttt tggatttggt aactatttac gaagaatttg gatacttcaa agggctaaaa
     9361 attgcgattg taggagattt aactcactca cgggtagcta aatctaacat gcaagtgcta
     9421 aaacggttag gagctgaaat tttcttttct ggaccaaaag aatggtatag tagtcagttt
     9481 gatgaatatg ggcaatattt accaatagac cagttagttg atcaaattga tgtccttatg
     9541 cttttacgtg ttcaacacga acgtcatgat ggtaaagggg tgttttcaaa agaaagctat
     9601 catcaacaat ttggtcttac aaaagaacgc tataaacatt taagggatac agcaattatt
     9661 atgcacccag cgcccgtcaa tagggatgta gagattgcta gcgatttggt agaagcagat
     9721 aaggctcgaa tagtgaaaca gatgagtaat ggcgtctatg caaggatagc tatcttagaa
     9781 gcagtgctca atagcagaca ataaaggaga taaaatgaag cgtctattat tactggaaga
     9841 tggtagtgtg tttgagggtg aggctttcgg agcggatgta gaaacaagtg gtgaaatcgt
     9901 ttttagtaca ggaatgacag ggtatcaaga atctataaca gatcaatctt acaacggtca
     9961 aatcattact tttacttatc ctcttattgg aaattatggg ataaatcgag acgattacga
    10021 atctatcaga ccaacatgta agggagtagt tgtttatgaa tgggcagagt atccaagtaa
    10081 ctggcgtcaa cagatgacac ttgatgaatt tttaaaatta aaaggaatac ctggaatttc
    10141 aggtatagat actcgagcat taacaaaaat tattcgaaaa catggaacaa tgaaagcttg
    10201 tttaataaac gagggaaatt ctattcatga agcactagaa aaccttcaag aaagtgttct
    10261 tttaaataat caaattgaac aagtatcaac caaattagct tatgcttcgc ctggagttgg
    10321 caaaaatatt gtattagttg actttggttt aaaacattct attctaagag aactgtcaca
    10381 acgtcaatgt catatcacag tgatccctca tactacgaca gcgcaagaaa tcttaaatct
    10441 caatccagat ggagtactct tatctaacgg ccctgggaac ccagaacaat taccgaatgc
    10501 tttacaaatg atccaagaaa ttcaaagtaa aattccaatt tttggtattt gtatgggaca
    10561 tcaactattt gctaaagcca atggtgcaaa gacttataaa atgacttttg gtcatcgagg
    10621 ttttaatcat gctgttcgtc atttgcaaac aggacaggtt gattttacaa gtcaaaatca
    10681 tggttatgct gtttcgagag aagatttccc tgaggctctc ttcattacac atgaagagat
    10741 taatgataaa accgtcgaag gtgttcgaca taaatactat cctgcctttt cagtgcagtt
    10801 tcacccagat gcagcaccag ggccgcatga tacatcttat ctttttgatg aatttattaa
    10861 tatgattgat gatttccagc aaaaaagcta gctttaaaat atagaattag aggagtttga
    10921 tatgccaaag cgcacagaca tacgtaaaat tatggttatc ggttctggcc cgattgtgat
    10981 tggacaagca gcagagtttg actattctgg tacacaagcc tgtctctctt taaaagagga
    11041 gggataccaa gttgttttgg tcaattcgaa tccggctact atcatgacag ataaagatat
    11101 tgctgataaa gtttatattg aacctattac cttggagttt gtcacaagaa ttctcaggaa
    11161 ggaaagacct gatgctctcc ttccaactct tggaggtcaa actggattga atatggctat
    11221 ggctctatca aaaaatggta tcctagaaga attgaatgtt gaactattag ggaccaaatt
    11281 atcagccatt gataaagctg aagatcgtga tttatttaaa cagttgatgg aagaactcaa
    11341 tcaacctatt ccagaatccg agattgttaa ttcggtagaa gaagctattc aatttgcaga
    11401 gcaaatcggg tatccattaa ttgttcgtcc tgcctttact ttaggaggta caggtggagg
    11461 catgtgcgat aatcaagaac aattggttga catcacgacg aaggggttaa agttatcgcc
    11521 tgtgacacag tgccttattg aacgttcaat tgccggcttt aaagagattg aatacgaagt
    11581 tatgcgtgat gcagcagata acgctcttgt tgtttgtaat atggaaaatt ttgatcctgt
    11641 tggtatccat acaggggact ctatagtttt tgcacccgca caaacgttat ctgatgtgga
    11701 aaatcagtta ttacgagatg cgagtttaga tattataaga gctttgaaaa ttgaaggagg
    11761 atgcaatgtt caactagccc ttgatccgaa tagttttaaa tactatgtca ttgaagttaa
    11821 tccaagagta tcaagatctt ctgctttagc ttcaaaagct actggatatc caattgctaa
    11881 attagcagct aagatagctg ttggtttgac actagatgag gtaattaatc ctattacaaa
    11941 aacgacttac gctatgtttg aacctgcttt agattacgta gtcgcaaaaa tgccgagatt
    12001 tccttttgat aaatttgaaa gtggcgatag aaaattggga acacagatga aagctacagg
    12061 cgaagtcatg gcaataggac ggaatataga agaatctttg ttgaaagctt gtcgttcact
    12121 agaaataggt gtcgatcaca ttaaaatagc agatttagat aatgtatcag atgatgtttt
    12181 attagaaaaa ataagaaagg cagaagatga tcgcttattt tatctagcag aagctttacg
    12241 tagacattat agtattgaaa aattagcaag tttgactagc attgattctt tcttccttga
    12301 taagttaaga gtgattgtag agttagaaga tctattatct aaaaacagac tggatatcaa
    12361 tattctcaaa aaggttaaaa ataaaggttt ttcagataaa gctatagcga gtttatggca
    12421 aataaacgaa gatcaagttc gtaacatgcg aaaagaagca ggaattcttc cagtttataa
    12481 gatggttgat acatgtgcgt ctgagtttga ttcagcaacg ccctactttt attcgactta
    12541 tgccgtagaa aacgagtcat tgatatcaga taaagcctct attttggttt taggatcggg
    12601 cccaatccga attggacagg gagttgaatt tgattatgca acggttcatt ctgttaaagc
    12661 catcagagag tctggttttg aagcgattat catgaactct aatccagaga cggtctcaac
    12721 ggatttctct atttcagata agctttactt tgaacctttg acttttgaag atgttatgaa
    12781 tgttattgac ttagaaaaac ctgaaggggt catcttacaa ttcggtggtc agactgctat
    12841 taatctagca aaagatttaa acaaggcagg ggttaaaata ctaggcacac aattagaaga
    12901 tttggatcgt gctgaaaata gaaaacaatt tgaagccact ttacaagctc taaatatccc
    12961 tcaaccacca ggttttaccg ccactacgga ggaagaggca gttaatgcag cgcaaaagat
    13021 tggttatccc gtgttggtta gaccatcata tgttcttggt ggacgagcta tgaagatagt
    13081 tgaaaacgaa gaagatttac gtcattatat gacaaccgcg gtaaaggcaa gcccagatca
    13141 tccagtgctt atagatgcat accttatagg taaagagtgt gaggttgatg ctatatccga
    13201 tggtcagaat atactgattc ctggaataat ggagcatata gagcgagctg gtgttcattc
    13261 aggagattcc atggctgtat atcctcctca aacattgtct gagaccatta tcgaaactat
    13321 tgtagattat acaaaacgct tagcaattgg attaaactgt atcggtatga tgaatattca
    13381 gtttgtaatc aaggatcaga aagtatatgt tattgaagtt aatcctcgtg ctagtagaac
    13441 acttccattc ttatcaaaag taactcacat tcctatggcc caagtagcaa caaaagttat
    13501 cttaggagat aaactctgca attttacata tggttatgat ctctatccag cttcagatat
    13561 ggtccacatt aaagcccctg tctttagttt taccaaactt gctaaagtag atagtttatt
    13621 aggtcctgaa atgaaatcaa caggcgaagt gatgggatca gatattaatc ttcagaaagc
    13681 cttgtataaa gcctttgaag cagcatatct ccatatgcca gactatggta acattgtttt
    13741 tacagtagat gatacagata aagaagaagc tttagaactt gcaaaagtct atcaaagtat
    13801 tggttatcgt atttatgcta cccaagggac tgcaatttat tttgatgcta atggtttaga
    13861 gacagtttta gtaggtaagt taggagagaa cgatcgaaat catattcctg atttaattaa
    13921 aaatggtaag attcaagcag ttatcaacac agttggacaa aataacattg ataaccatga
    13981 tgctctcatc atcagacgtt ctgcaattga acaaggagtt cctctattta catctttaga
    14041 tactgcacat gcaatgttca aagttcttga aagcagagca tttacattaa aagtactaga
    14101 ttaatcaaac gatcaaggta aaattaactt ccagcaaaac agaatcggac tagattaaat
    14161 atagcttgtt ctgtttttat aaaaaaagcc taattgtgat aaaataggga acaaacttaa
    14221 ggagttaatt atgttaaaaa cacgaacaca tattaatgaa ttagaaacaa aaatcgccct
    14281 tgatttagta cacagatatc taggtattca acaacttgtt ttttcaaagt ggaggcattc
    14341 tttagaaact ggttcatggt cttctattat aacaataaat ggcgttaata ttatcaatta
    14401 cattgttgat gatattagtg acagtaatag tatttcaggt attatttgtc atcctaaaag
    14461 ttttcatctc agacctagag aaaaaaagga acctattcag tctattgatg agttagatat
    14521 cacctggtct ttgaatagag aggaaaatga atgattccag atagtagttt taaagtgcta
    14581 tcagatatgg catataatat tgaaaaatcc aaagcagaaa gtaatggaca ccttcaaatt
    14641 atagaaggta gtcaaattat tgatttccat caaaaacgct cttttatggt attagaggtt
    14701 aatgatgata tcaataatgg tatgcaggct atggcatgtc agtctttgga tgaaagtaat
    14761 catattgtta tagcatattc aggaacgaac gcttttaata agaaagatat cttaacagat
    14821 atcgattcag ttgtattggg gaagaacaat aaattgctaa cttgtccttc aagtattacc
    14881 ctgagagagg atggcatttc tctgccttgt aaaaatccaa gtcaaattac aacatctaaa
    14941 ctatttgcac aaaaaattaa gatgaactat cctgatgcaa ccctctggtt aacaggccat
    15001 tctctaggtg gttatttggc catgattgtg gcttcagaaa tgagattatc agcgcgtgtt
    15061 tttaattccc ctagtcccat taacaatatg tctgaagcag ccacatccta tgtaatttca
    15121 catccagaga agtatcatag ttaccgtatt aataaagatt acattggtaa ttttggtatt
    15181 aatagttcag atgataaatt aggtatttct aaaagagtca atgctaagaa gcaacatggt
    15241 ttttataaaa agattttctt tcacgaattg acacgatttc gattaaatca acaaggacag
    15301 attgttgata tagatgaaaa taaacaggat taaacaataa tattaaaaat aattttaaat
    15361 aatgcagtaa atttgaatat attttaaaaa atatgttgta ataattatgc agttgttgca
    15421 aaagtgttta tagaaaaagg agaaaattta tggcacaaat taaattaaca ccagaagaac
    15481 ttcgctcatc agcacaaaag tacacagctg gttctcaaca agtaacagaa gttcttaatt
    15541 tgcttactca agagcaagca gttattgacg aaaattggga tggatctact tttgattctt
    15601 ttgaagcaca attcaatgaa ttatctccaa aaattacaga atttgctcaa ttactcgaag
    15661 atattaacca gcaattatta aaagttgcag atatcattga gcagacggat gcagatattg
    15721 cttctcaaat tagtggatag tatcgtatca tagtctataa gtgaatagga aggcaaagcc
    15781 ttcctatttt gttgaaaata aaatatattt taggaagaat tatgaaaaga aataacttta
    15841 gaatactttg gtatattata gctgttgctc tctttttagt agctattgct ggccttaatc
    15901 ttaagcttca aggcgatcat gcaaaagaga ataaaacaac acagtcagct actaacacta
    15961 aactaaatat agcattagtc aacgaagatc aaaatgtatc taatggtaaa gaaagttata
    16021 atttaggagc tagttatatt aagtctatcg aacgtgataa tagtcaaaat tggtcagttg
    16081 ttagtcgagg tactgctcaa aatggattag acaaaggtga ttatcaatta atggttatca
    16141 tccctaataa cttttctcaa aaacttcttg atgtcaataa agcaaatgca gagcaaacaa
    16201 ctatctctta taaggttaat gccaagggga atttagcatt agagaaaaag gcaactgaaa
    16261 aagagaaaga tattgtttca gagttaaata gccacttagt aaatatgtac atggcaagta
    16321 ttttaagtaa cttatataca gctcaagaaa atgtacaggc catggtaaat gttcagtcag
    16381 gtaatatctc aaattaccaa aaaaatcttt tagattctgc aactaatttc caaaatatct
    16441 ttccagccct cgttaaccag tctagtagtt ccattactgc taacgaatca ctgaaaaaat
    16501 ctttagaagc ttctgataac atgtttaatg atttggtgac aacccagaca aatactggaa
    16561 aagatttatc aagcttaata gaacagcgcc atcaagatag catttcgtat gaagcgtttt
    16621 cgacctcatt actagaaatg aataacgagt tattagagaa gcaattatct gatattatca
    16681 cacaagcaca aaaagaccaa gaaacattat catcacaact caatagtatt atgggtgatg
    16741 ataacaatca taatcataaa gaaaactcat cagcttatct aaatgttgca aggcaaaaaa
    16801 tccaagaact atctgaagca ctcaagtcac aagataacat tgctaaagat caaagtgaac
    16861 aactagataa aattgttaga gagggactag caagttactt tgctaagaat aataaagata
    16921 atattacttt attagaatta ttgaagagtc attctactaa tgagaagact ttgaaagatt
    16981 ttaaagctaa ggtagcagat ttcacaaatt ctctcatttc aagcattcct tcactaaacc
    17041 tttcggagtt gcacttaacc caagaagaag aaaaagccat tcagtttaca tcttctgatt
    17101 cggaaatcat taaaaaagta agcaacgaaa gaactctgta tttaaatact aatttattga
    17161 atcgtctttt tgaagctagg aaaaatagag atgaagctaa aataaagtta atcaattaaa
    17221 attgtcatct agttctactc gaactggtga gcagatagtt tctgttgaat caaataatcc
    17281 agattacagg gtagatacct ggacggttaa tggtaagcaa acaagaacct tggaccccag
    17341 ccaaaacaat aatattatta tcaatagcag ttatcaaagg gaatcatcta actctagtat
    17401 tacgaaacca agctatacaa tcactatcgg gaatcagaaa caaattgtac aaaatgatga
    17461 cggtaaaatc caacggtctt actttgaggc agaggcaacg tatcaacgaa ctctacaaga
    17521 agtaaatgat gcatataaca ccactcacgg attagtagct aaatactaca ttatttctga
    17581 tggcgaagaa ccgcaaaatt tatttgatca atttttaaat caaagtgtta acgatacgat
    17641 ggttgatctg gtcaaaaatg gtataactaa gtatttgatg gatgaaaaca ctgctgatgc
    17701 gcaacataaa gttaaagatg tcatggagga agttgaaaat agtcaagatg aattggcgga
    17761 tcaaatggca aaagtaactg agacaaatgt acgactaaca gatgcaatca aaaaacagct
    17821 tgaaaccctt caatcaatta acatgaaggt ccaaaacatc acacaggatc aatctaaagt
    17881 aaacgactca cagaaaacaa ctgatcaaca attatctgat ttgaagaacc atctagatgg
    17941 gctaatgacc tctgcagcgg gcgtaaaaga tatgtccaaa tctaatagtc aagaggcgga
    18001 ccaagttaat caaatattca catcattcaa taaagatgtt caagatgcta aaaattcggg
    18061 caacaaactt tcgacggatg caaccgattt aatggctaac ttccaaaaag aattggccaa
    18121 taatggtgat ttcgtagctt ctttctctaa agtttttaat actgcttata aaaatggagt
    18181 gccaaatgat attctcctta atttcttatc acgacctgtc gctgaatcag cgtctgcagt
    18241 tagggcaact gaaaatactt atcgcccatt cacttggata ctattattag aagttgttag
    18301 cctatttaca gcatatatct ttgcgactca aaaccttatt aagaaattga cagataggta
    18361 taatgtcaac cgctggcttc aaacagactt tttaaatgtc attgtcattt caggcctctc
    18421 tcttgttatt ggtttagcac tgggagttat ctcaagcaga agtcttcatg tcatgcctga
    18481 atatgtacca tcttggttcc tagttatgac tctgtttagt tttttattaa ttcatagtca
    18541 gtatttcttt attaaaaatt ttaaagcagt tggtatgggt ttggctttgt ttatgattat
    18601 tagttttgtg tatctatcaa atgccgtagg cacagtagcg actgttagtg gacttccaaa
    18661 attactaaaa gctattaatc cactatctat ccttgaaaat caattatcat cttattttga
    18721 taatgtgaca actggattta ttttccttat cttagtactt cttgtggatg ttgcttttat
    18781 tatcatgaat atttttatca ccttaaattt tgaagctaaa gtaaaagagt gaggttgttg
    18841 atgaagttga aacgattttt agcctgtcta ctaatattat cctgtatgag tcaaacatca
    18901 gtattttctg atgggttaaa gaataatgat attcattttg atacaaaacg tttagaatca
    18961 gattcacaaa aaaattctgg ttttcaaact gaattaacta aagatttatt taaaaaagaa
    19021 gaccttaaaa aattggataa aaaccgtcaa tctaaagtct cactccagaa aaagcagtct
    19081 aagcaactgt ttcaatattc aaataaagga caatctgata ggaaaaaacg tatcaaacaa
    19141 ttattttctg aagaaagtaa tcaatttaaa tttgttacaa gaacagaaga accgactgtt
    19201 tcggctgata ttggtcatat aattatatac ctggttctca ttagtttggt actaatatta
    19261 gcttctataa tgacttttaa aataaagga
//
LOCUS       DATRTN010000028        18620 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.028, whole genome shotgun sequence.
ACCESSION   DATRTN010000028 DATRTN010000000
VERSION     DATRTN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18620
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.028"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            186..4319
                     /gene="cas9"
                     /locus_tag="VBE22_001014"
     CDS             186..4319
                     /gene="cas9"
                     /locus_tag="VBE22_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040090.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEO7202483.1"
                     /translation="MNKPYSIGLDIGTNSVGWSIITDDYKVPAKKMRVLGNTDKEYIK
                     KNLIGALLFDGGNTAADRRLKRTARRRYTRRRNRILYLQEIFAEEMSKVDDSFFHRLE
                     DSFLVEEDKRGSKYPIFATMQEEKYYHEKFPTIYHLRKELADKKEKADLRLVYLALAH
                     IIKFRGHFLIEDDRFDVRNTDIQKQYQAFLEIFDTTFENNHLLSQNVDVEAILTDKIS
                     KSAKKDRILAQYPNQKSTGIFAEFLKLIVGNQADFKKHFNLEDKTPLQFAKDSYDEDL
                     ENLLGQIGDEFADLFSVAKKLYDSVLLSGILTVTDLSTKAPLSASMIQRYDEHHEDLK
                     HLKQFVKASLPENYREVFADSSKDGYAGYIEGKTNQEAFYKYLLKLLTKQEGSEYFLE
                     KIKNEDFLRKQRTFDNGSIPHQVHLTELRAIIRRQSEYYPFLKENQDRIEKILTFRIP
                     YYVGPLAREKSDFAWMTRKTDDSIRPWNFEDLVDKEKSAEAFIHRMTNNDLYLPEEKV
                     LPKHSLIYEKFTVYNELTKVRFLAEGFKDFQFLNRKQKETIFNSLFKEKRKVTEKDII
                     SFLNKVDGYEGIAIKGIEKQFNASLSTYHDLKKILGKDFLDNTDNELILEDIVQTLTL
                     FEDREMIKKCLDIYKDFFTESQLKKLYRRHYTGWGRLSAKLINGIRNKENQKTILDYL
                     IDDGSANRNFMQLINDDDLSFKPIIDKARTGSHSDNLKEVVGELAGSPAIKKGILQSL
                     KIVDELVKVMGYEPEQIVVEMARENQTTAKGLSRSRQRLTTLRESLANLKSNILEEKK
                     PKYVKDQVENHHLSDDRLFLYYLQNGRDMYTKKALDIDNLSQYDIDHIIPQAFIKDDS
                     IDNRVLVSSAKNRGKSDDVPSIEIVKARKMFWKNLLDAKLMSQRKYDNLTKAERGGLT
                     SDDKARFIQRQLVETRQITKHVARILDERFNNEVDNGKKICKVKIVTLKSNLVSNFRK
                     EFGFYKIREVNDYHHAHDAYLNAVVAKAILTKYPQLEPEFVYGMYRQKKLSKIVHEDK
                     EEKYSEATRKMFFYSNLMNMFKRVVRLADGSIVVRPVIETGRYMGKTAWDKKKHFATV
                     RKVLSYPQNNIVKKTEIQTGGFSKESILAHGNSDKLIPRKTKDIYLDPKKYGGFDSPI
                     VAYSVLVVADIKKGKAQKLKTVTELLGITIMERSRFEKNPSAFLESKGYLNIRDDKLM
                     ILPKYSLFELENGRRRLLASAGELQKGNELALPTQFMKFLYLASRYNESKGKPEEIEK
                     KQEFVNQHVSYFDDILQLINDFSKRVILADANLEKINKLYQDNKENIPVDELANNIIN
                     LFTFTSLGAPAAFKFFDKIVDRKRYTSTKEVLNSTLIHQSITGLYETRIDLGKLGED"
     gene            4321..5190
                     /gene="cas1"
                     /locus_tag="VBE22_001015"
     CDS             4321..5190
                     /gene="cas1"
                     /locus_tag="VBE22_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO7202484.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLIFKDSYQTEMIHLSEIDILIME
                     TTDIVLSTMLIKRLVDENILVIFCDDKRLPTAMLMPYYARHDSSLQLSRQMSWIEDVK
                     ADVWTSIIAQKILNQSFYLGECSFFEKSQSIMNLYHDLESFDPSNREGHAARLYFNTL
                     FGNDFSREQDNPINAGLDYGYSLILSMFAREVVKCGCMTQFGLKHANQFNQFNLASDI
                     MEPFRPIVDRIIYENRQSDFVKMKRELFSMFSETYSYNGKEMYLSNIVSDYTKKVIKS
                     LNSDGNGIPEFRI"
     gene            5187..5528
                     /gene="cas2"
                     /locus_tag="VBE22_001016"
     CDS             5187..5528
                     /gene="cas2"
                     /locus_tag="VBE22_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEO7202485.1"
                     /translation="MSYRYMRMILMFDMPTETVEERKAYRKFRKFLLSEGFIMHQFSV
                     YSKLLLNNTANNAMIGRLKVNNPKKGSITLLTVTEKQFARMVYLHGEHNMSVANSDSR
                     LVFLGDSYDQD"
     gene            5515..6180
                     /gene="csn2"
                     /locus_tag="VBE22_001017"
     CDS             5515..6180
                     /gene="csn2"
                     /locus_tag="VBE22_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEO7202486.1"
                     /translation="MIKINFPILDEPLVLSNATILTIEDVSVYSSLVKHFYQYDVDEH
                     LKLFDDKQKSLKATELMLVTDILGYDVNSAPILKLIHGDLENQFNEKPEVKSMVEKLA
                     ATITELIAFECLENELDLEYDEITILELIKALGVKIETQSDTIFEKCFEIIQVYHYLT
                     KKNLLVFVNSGAYLTKDEVIKLCEYINLMQKSVLFLEPRRLYDLPQYVIDKDYFLIGE
                     NMV"
     repeat_region   6281..7042
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6281..6316
                     /rpt_unit_seq="gttttagagctgtgctgtttcgaatggttccaaaac"
     gene            7095..7355
                     /locus_tag="VBE22_001018"
     CDS             7095..7355
                     /locus_tag="VBE22_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202487.1"
                     /translation="MLGHHCGVLVFCYTEINFQRMWGKAVIRLIQDVIQNLVGQANEI
                     TPIYQFDWETYILATKKYERHLEVCLLVENSNCFSDSKRMCR"
     gene            7363..7506
                     /locus_tag="VBE22_001019"
     CDS             7363..7506
                     /locus_tag="VBE22_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202488.1"
                     /translation="MKGYNSNLMVKGQDCSTLYLIISLTALVLLVIAGVSLSLILASL
                     QGK"
     gene            7665..8081
                     /gene="ndk"
                     /locus_tag="VBE22_001020"
     CDS             7665..8081
                     /gene="ndk"
                     /locus_tag="VBE22_001020"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEO7202489.1"
                     /translation="MEQTFFMIKPDGVKRGFIGEVISRIERRGFSIDRLEVRHADADI
                     LKRHYAELTDRPFFPTLVDYMTSGPVIIGVISGEEVISTWRTMMGSTNPKDALPGTIR
                     GDFAQAPSPNQATCNIVHGSDSPESATREIAIWFNN"
     gene            8217..10049
                     /gene="lepA"
                     /locus_tag="VBE22_001021"
     CDS             8217..10049
                     /gene="lepA"
                     /locus_tag="VBE22_001021"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEO7202490.1"
                     /translation="MNIEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRAEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGEVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGMVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVAD
                     TRVGDTITLANNPAIEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVNTTDGEMLE
                     VSNPSEFPDPTRVDSIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDDKK"
     gene            10295..12928
                     /locus_tag="VBE22_001022"
     CDS             10295..12928
                     /locus_tag="VBE22_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO7202491.1"
                     /translation="MKGQKIIALAGLVLSCHFALTACHTQEHKNSHHIKTKQVAKKKA
                     NKKKVSVKESHKKRKGVAGVDFPTDDGFLLTKDSKILSHTDSGIVVAHGNHSHFIFYS
                     DLKGSKFSYLIPNANAKTNKKQAVRNFKAGAVAVNTLNDGYVFNPADIVSEDANGYVV
                     RHGDHFHYIPKASLSQQKQVQASRAVSRLGNQNNSHYRVNSSKIAGLHHPTSDGFLFN
                     GQGIKGTTPTGILVEHHNHLHFISFADLRKGGWGSIADRYQPQKKVDSKKQSPSSKKP
                     RTENTLPKDIKDKLAYLARELHLDISRIRVLKTLNGEIGFEYPHDDHTHVIMAKDIDL
                     SKPIPNPHHDDEDHHKGHHHDESDHKHEEHEHTKSNKLSDEDQKKLIYLAEKLGLNPN
                     QIEVLTSEDGSIIFKYPHDDHSHTIASKDIEIGKPIPDGHHDHSHAKDKVGMATLKQI
                     GFDDEIIQDILHADAPTPFPSNETNPEKMRQWLATVTKINIGQRTNPFQRFGLSLMPN
                     IEVLGIGFTPINDMTPVLQFKKLKQLWMTNTGITDYSFLDKMPLLEGLDISQNGIKDL
                     SFLTKYKQLSLIAAANNGITSLKPLAELPNLQFLVLSHNNISDLTPLSNLTKLQELHL
                     DHNNVKNLSALSGKKDLKVLDLSNNKSADLSTLKTTSLETLLLNETNTSNLSFLKQNP
                     KVSNLTINNAKLASLDGVEESDEIVKVEAEGNQIKSLVLKNKQGSLKFLNVTNNQLTS
                     LEGVNNYTSLETLSVSKNKLESLDIKTPNKTVTNLDFSHNNVPTSQLKLNEKNIPEAV
                     AKNFPAVVEGSMVGNGSLAEKAAMASKEDKQVSDNTNHQKNTEKSAQANADSKKENPK
                     THDEHHDHEETDHAHVGHHHH"
     gene            13028..13687
                     /locus_tag="VBE22_001023"
     CDS             13028..13687
                     /locus_tag="VBE22_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO7202492.1"
                     /translation="MTMVKREDVILNATENMVYHKLKNDPSGHDWFHIVRVRNLAVEL
                     AHKEGANTFICQMAALLHDIIDDKICQDSKQASYELTQWLYSQDLAIAEVEHILDILE
                     NISFKAGTGLTMKTLEGQIVQDADRLDAMGAIGIARTMAYSGSKGRLIHDPNLKPREN
                     LTLEEYRNGQDTAIMHFYEKLLKLKDLMNTKQGKMLAQKRHDFLELYLAEFYAEWNGK
                     G"
     gene            13696..14160
                     /locus_tag="VBE22_001024"
     CDS             13696..14160
                     /locus_tag="VBE22_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7202493.1"
                     /translation="MIRRAKEKDLPAIAELLKQILVLHHGVRPDIFHTRGSKFSKEQL
                     KEMLMDESRPIFVYESDEGKVVAHLFLQLQEKRDLPRKSFKTLYIDDLCIDEEVRGQQ
                     IGQKLMDFARQYAKKHGCYNITLNVWNDNQRAVSFYEKLGFKPQQTQMEEIL"
     gene            14160..14594
                     /gene="msrB"
                     /locus_tag="VBE22_001025"
     CDS             14160..14594
                     /gene="msrB"
                     /locus_tag="VBE22_001025"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEO7202494.1"
                     /translation="MKETQEELRQRIGHTAYQVTQNSATEHAFTGKYDDFFEEGIYVD
                     IVSGEVLFSSLDKFQSGCGWPAFSKPIENRMVTNHQDHSHGMHRIEVRSRQADSHLGH
                     VFNDGPVDAGGLRYCINSAALDFIPYDQMAKRGYGDYLSLFD"
     gene            14746..17538
                     /locus_tag="VBE22_001026"
     CDS             14746..17538
                     /locus_tag="VBE22_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEO7202495.1"
                     /translation="MASTNNVSDTNTCFAKEELEEIFEELGTTQGGLSDEEVAVRQKK
                     YGLNLLSEVEQESIILLFLKNFTSLMAILLWVGGFVAIVSNSLELGLAIWMVNVINGI
                     FSFIQEYRASQATQALKKMLPSYSRVLRKSSEEKILSEQLVPGDIVLIEEGDRISADG
                     RLIKTTDLQVNQSALTGESNPIYKDSNVENDQSKTLIECDNMVFAGTTVSSGSATMVV
                     TAIGMQTQFGQIADLTQGMKSEKSPLQRELDRLTKQISIISITVGIIFFLAATFFVKE
                     PVSKSFIFALGMIVAFIPEGLLPTVTLSLAMAVQRMAKEHALVKKLSSVETLGATSVI
                     CSDKTGTLTQNEMTVNHLWQNGKSYQVTGLGYAPEGQILFEGDNICFGNSDRGDLEKL
                     IRFAHLCSNAQVLPPNDERSTYTVLGDPTEACLNVLLEKSGINRQENRKFAPRLKELP
                     FDSVRKRMTTIHSLGGDEKDKKISITKGAPKEILDLSDYVLSDGKVIPLNKEERNKIQ
                     LANDTFAKDGLRVLAVSYCDIEGFSKEQWTQENLEQHMVFIGLIAMSDPPREGVREAI
                     DKCHAASIRIIMVTGDYGLTALSIAKNIGIIRNDGAKVISGLELSEMTDSQLKKELSG
                     EAVFARVAPEQKYRVVTILQEMGEVVAVTGDGVNDAPALKKSDIGVAMGVTGTDVAKE
                     SADMILTDDHFASIVHAVEEGRAVYQNIKKFLTYIFNSNTPEAVPSAFFLFSKGFIPL
                     PLTVMQILAVDLGTDMLPALGLGVEPPETDVMNRPPRRLTDRLLDKGLLIKSFLWYGT
                     IESVLAMGGFFWAHYLRYGNFTFFVANGIPYREATTMTLGAIIFSQIGMVMNSRTSYQ
                     SIKTLSIFGNKLINFGIIMEILAFLVLVYVPLFHNLFNTASLGLSHWLYLIGCPFIMI
                     GLDEVRKLFSSRKNKR"
     gene            17538..>18620
                     /locus_tag="VBE22_001027"
     CDS             17538..>18620
                     /locus_tag="VBE22_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO7202496.1"
                     /translation="MSNIITYLKNNSNLTFDELALNDVDILCLNEFGYISFEKLINTT
                     EMKSVLVCELYHEYLQTMAKSYSFMFTSQRHDLCQLMMTSKRFKNLTLSYYRAEISLE
                     FEKQFAAMVFTIPNINYHQVVFRGTDANLIGWKEDFKLTYMREISAHRSAIKYLNTIL
                     PYFDKVVLSGHSKGGNLALYAAMFTKPDLKAKIDLIWLIDSPGLQKTLLPTTEYKTTK
                     QKCIRLLPEESIVGMMLYSDIEPLIISSNARGILQHDVTTWEIQEPAILKTGTGLSLK
                     SICFEKTFQQWMAELKSQERKLFFDLLFDSFLSSGVSSLDDFNLASRAKMMKAFHSFR
                     ELDDDKKRLFNKSLKLLVTIFLGAYHD"
BASE COUNT         6335 a         2842 c         3630 g         5813 t
ORIGIN      
        1 ataatcaagt aaaaaataaa aatcagcaaa gctttaaaac ttaaaacata ctttaggata
       61 ttttaacaaa taacagatga aagataaaga taatgctttt aaaactcaaa aaaataatat
      121 ataattaaag tatgttttaa aggtgttgtt tttagaatgg tgttttaaat aaaaggagaa
      181 aagttatgaa taagccatat tcaataggcc ttgacatcgg tactaattcc gtcggatgga
      241 gcattattac agatgattat aaagtacctg ctaagaagat gagagtttta gggaacactg
      301 ataaagaata tattaagaag aatctcatag gtgctctgct ttttgatggc gggaatactg
      361 ctgcagatag acgcttgaag cgaactgctc gtcgtcgtta tacacgtcgt agaaatcgta
      421 ttctatattt acaagaaatt tttgcagagg aaatgagtaa agttgatgat agtttctttc
      481 atcgattaga ggattctttt ctagttgagg aagataagag aggtagcaag tatcctatct
      541 ttgcaacaat gcaggaggag aaatattatc atgaaaaatt tccgacaatc tatcatttga
      601 gaaaagaatt ggctgacaag aaagaaaaag cagaccttcg tcttgtttat ctggctctag
      661 ctcatatcat taaattcaga gggcatttcc taattgagga tgatagattt gatgtgagga
      721 ataccgatat tcaaaaacaa tatcaagcct ttttagaaat ttttgatact acctttgaaa
      781 ataatcattt gttatctcaa aatgtagatg tagaagcaat tctaacagat aagattagca
      841 agtctgcgaa gaaggatcgc atcttagcgc agtatcctaa ccaaaaatct actggtattt
      901 ttgcagaatt tttgaaattg attgtcggaa atcaagctga cttcaagaaa catttcaatt
      961 tggaggataa aacaccgctt caattcgcta aggatagcta cgatgaagat ttagaaaatc
     1021 ttcttggaca gattggtgat gaatttgcag acttattctc agtagcgaaa aagctatatg
     1081 atagtgttct tttatctggc attcttacag taactgatct cagtaccaag gcgccacttt
     1141 ctgcctctat gattcagcgt tatgatgaac atcatgagga cttaaagcat ctaaaacaat
     1201 tcgtaaaagc ttcattacct gaaaattatc gggaagtatt tgctgattca tcaaaagatg
     1261 gctacgctgg ctatattgaa ggcaaaacta atcaagaagc tttttataaa tatctgttaa
     1321 aattgttgac caaacaagaa ggtagcgagt attttcttga gaaaattaag aatgaagatt
     1381 ttttgagaaa acagagaacc tttgataatg gctcaatccc gcatcaagtc catttgacag
     1441 aattgagggc tattattcga cgtcaatcag aatactatcc attcttgaaa gagaatcaag
     1501 ataggattga aaaaatcctt acctttagaa ttccttatta tgtcgggcca ctagcacgtg
     1561 agaagagtga ttttgcatgg atgactcgca aaacagatga cagtattcga ccttggaatt
     1621 ttgaagactt ggttgataaa gaaaaatctg cggaagcttt tatccatcgc atgaccaaca
     1681 atgacctcta tcttccagaa gaaaaagttt taccaaagca tagtcttatt tatgaaaaat
     1741 ttactgttta caatgaatta acgaaggtta gatttttggc agaaggcttt aaagattttc
     1801 aatttttaaa taggaagcaa aaagaaacta tctttaacag cttgtttaag gaaaaacgta
     1861 aagtaactga aaaggatatt attagttttt tgaataaagt tgatggatat gaaggaattg
     1921 caatcaaagg aattgagaaa cagtttaacg ctagcctttc aacctatcat gatcttaaaa
     1981 aaatacttgg caaggatttc cttgataata cagataacga gcttattttg gaagatatcg
     2041 tccaaactct aaccttattt gaagatagag aaatgattaa gaagtgtctt gacatctata
     2101 aagatttttt tacagagtca cagcttaaaa agctctatcg ccgtcactat actggctggg
     2161 gacgattgtc tgctaagcta ataaatggca tccgaaataa agagaatcaa aaaacaatct
     2221 tggactatct tattgatgat ggaagtgcaa accgaaactt catgcagttg ataaatgatg
     2281 atgatctatc atttaaacca attattgaca aggcacgaac tggtagtcat tcggataatc
     2341 tgaaagaagt tgtaggtgaa cttgctggta gccctgctat taaaaaaggg attctacaaa
     2401 gtttgaaaat agttgatgag ctggttaaag tcatgggcta tgaacctgaa caaatcgtgg
     2461 ttgaaatggc acgtgagaac caaacgacag caaaaggatt aagtcgttca cgacaacgct
     2521 tgacaacctt gagagaatct cttgctaatt tgaagagtaa tattttggaa gagaaaaagc
     2581 ctaagtatgt gaaagatcaa gttgaaaatc atcatttatc tgatgaccgt cttttccttt
     2641 actacttaca aaacggaaga gatatgtata caaaaaaggc tctggatatt gataatttaa
     2701 gtcaatatga tattgaccac attattcctc aagctttcat aaaagatgat tctattgata
     2761 atcgtgtttt ggtatcatct gctaaaaatc gtggaaaatc agatgatgtt cctagcattg
     2821 aaattgtaaa agctcgcaaa atgttctgga aaaatttact ggatgctaag ttaatgagtc
     2881 agcgtaagta tgataatttg actaaggcag agcgcggagg cctaacttcc gatgataagg
     2941 caagatttat ccaacgtcag ttggttgaga ctcgacaaat taccaagcat gtagctcgta
     3001 tcttggatga acgcttcaat aatgaagttg ataatggtaa aaagatttgc aaggttaaaa
     3061 ttgtaacctt gaagtcaaat ttggtttcaa atttccgaaa agaatttgga ttctataaaa
     3121 ttcgtgaagt taatgattat caccatgcac acgatgctta tcttaatgca gtagttgcca
     3181 aagctattct aaccaaatat ccacagttag agccagagtt tgtctacgga atgtatagac
     3241 agaaaaaact ttcgaaaatc gttcatgagg ataaggaaga aaaatatagt gaagcaacca
     3301 ggaaaatgtt tttctactcc aacttgatga atatgttcaa aagagttgtg aggttagcag
     3361 atggttctat tgttgtaaga ccagtaatag aaactggtag atatatggga aaaactgcat
     3421 gggataaaaa gaaacacttt gcgacagtta gaaaagtctt gtcataccct cagaacaata
     3481 tcgtgaagaa gacagagatt cagacaggtg gtttctctaa ggaatcaatc ttggcgcatg
     3541 gtaactcaga taagttgatt ccaagaaaaa cgaaggatat ttatttagat cctaagaaat
     3601 atggaggttt tgatagtccg atagtagctt actctgtttt agttgtagct gatatcaaaa
     3661 aaggtaaagc acaaaaacta aaaacagtta cggaactttt aggaattacc atcatggaga
     3721 ggtccagatt tgagaaaaat ccatcagctt tccttgaatc aaaaggttat ttaaatatta
     3781 gggacgataa attaatgatt ttaccgaagt atagtctgtt cgaattagaa aatgggcgtc
     3841 gtcgattact tgctagtgct ggtgaattac aaaaaggtaa cgagctagcc ttaccaacac
     3901 aatttatgaa gttcttatac cttgcaagtc gttataatga gtcaaaaggt aaaccagagg
     3961 agattgagaa gaaacaagaa tttgtaaatc aacatgtctc ttattttgat gacatccttc
     4021 aattaattaa tgatttttca aaacgagtta ttctagcaga tgctaattta gagaaaatca
     4081 ataagcttta ccaggataat aaggaaaata taccagtaga tgaacttgct aataatatta
     4141 tcaatctatt tacttttacc agtctaggag ctccagcagc ttttaaattt tttgataaaa
     4201 tagttgatag aaaacgctat acatcaacta aagaagtact taattctact ctaatccatc
     4261 aatctattac tggactttat gaaacacgta ttgatttggg taaattagga gaagattgat
     4321 atggcaggtt ggcgaactgt tgttgtaaat acacattcta agctctctta taaaaataat
     4381 catctgattt ttaaagattc ttatcagacg gaaatgattc atctttcaga gattgatatt
     4441 ctaatcatgg aaacgacaga tattgttttg tcgactatgc tgattaaacg tttggttgat
     4501 gaaaatattt tagtcatatt ttgtgatgat aaacgcttgc caacagctat gttaatgccg
     4561 tactatgcta gacatgattc gagtttacaa ttatctaggc agatgtcatg gattgaggat
     4621 gtcaaagcgg atgtttggac atcaattatt gcacaaaaaa ttttgaatca gtccttttat
     4681 ctcggtgagt gttctttctt tgaaaaatcc cagtctatta tgaatctcta tcatgattta
     4741 gaatcttttg acccttccaa tcgtgaaggt catgcagcta ggctttattt caatacactt
     4801 tttggaaatg atttttcaag agagcaggat aatccaataa atgctggttt agactatgga
     4861 tattctctga ttttgagtat gtttgcgcgt gaagttgtta agtgtggttg catgacacaa
     4921 tttggcttaa agcatgctaa tcaatttaat cagttcaacc tagcaagcga tattatggaa
     4981 ccatttcgcc caatcgttga taggattatt tatgaaaata ggcagagtga ttttgtcaaa
     5041 atgaaaagag aactcttttc tatgttttca gagacataca gctacaacgg taaagaaatg
     5101 tatctttcaa atattgtcag cgattacacc aaaaaagtta ttaagtcgct aaatagtgat
     5161 gggaatggaa ttccggagtt taggatatga gttatcggta tatgagaatg attttaatgt
     5221 ttgatatgcc tactgaaaca gtagaagaac gtaaggcgta tcgtaagttt agaaagtttc
     5281 tgttgagcga aggttttatt atgcatcagt tctctgttta tagtaaatta ttgctcaata
     5341 atacagctaa taatgccatg ataggtcggc ttaaagtgaa taatcctaag aaagggagta
     5401 taactctttt gacagttacc gagaagcagt ttgcaaggat ggtttatcta catggtgaac
     5461 ataatatgag tgttgccaac tctgatagtc gcttggtttt cctaggagat tcttatgatc
     5521 aagattaatt ttccaatttt agatgaacca ttagtgttaa gtaatgctac gattttaacg
     5581 atagaagatg tttcagttta ttcttcattg gtgaaacatt tttatcaata tgacgtagat
     5641 gaacatttga aattatttga tgataagcag aaaagtctga aggcaacgga gttaatgtta
     5701 gttacagata tcttaggata cgatgtcaac tcagcaccta ttctaaagtt gatacatggt
     5761 gacttagaaa atcaattcaa cgaaaagcca gaagtgaaat caatggtaga aaaattagca
     5821 gctactatta cagaacttat cgcatttgag tgtctagaga atgagcttga tttagaatac
     5881 gatgaaatta cgattttaga actcattaag gcactgggag tcaaaattga gacacagagc
     5941 gacactatct ttgaaaaatg ttttgaaatt atacaagttt accattattt aacgaaaaag
     6001 aatctcttag tttttgttaa tagcggagct tatcttacca aagatgaagt tataaaatta
     6061 tgtgaataca tcaatttaat gcaaaagtca gtactctttc tagaacctag aagactctat
     6121 gatttaccgc aatatgttat tgataaggat tatttcttga taggcgaaaa tatggtataa
     6181 tattagtaaa agcacagtaa taacaaggaa tcatcgaaac tgaagtcctg ctgagacgaa
     6241 tggcgcgatt acgaaagctc aaaagaaaat tttctacgag gttttagagc tgtgctgttt
     6301 cgaatggttc caaaacaaaa atactgtaaa atcgtaatct acataagttt tagagctgtg
     6361 ctgtttcgaa tggttccaaa acagacattt acctatcact ctagaaagtc atgttttaga
     6421 gctgtgctgt ttcgaatggt tccaaaacca tacaaggact accatcaaca gtcacattgt
     6481 tttagagctg tgctgtttcg aatggttcca aaacacgtac ccagcgatat actacgcaga
     6541 atgggtttta gagctgtgct gtttcgaatg gttccaaaac aaattgtttt tgttgtaata
     6601 taaagtcgac gttttagagc tgtgctgttt cgaatggttc caaaacaacc ttgataggcg
     6661 tactgtatgg attccagttt tagagctgtg ctgtttcgaa tggttccaaa acctactgta
     6721 ttatctgaaa tagtatcgtt ttgttttaga gctgtgctgt ttcgaatggt tccaaaactc
     6781 tcgggaaatg caactgatgc tgcaatcagt tttagagctg tgctgtttcg aatggttcca
     6841 aaacaaattg tttttgttgt aatataaagt cgacgtttta gagctgtgct gtttcgaatg
     6901 gttccaaaac ttaataactg tccgttttgc catttcttgc gttttagagc tgtgctgttt
     6961 cgaatggttc caaaacagag gggaaaatat caatgccgaa tgctgagttt tagagctgtg
     7021 ctgtttcgaa tggttccaaa acgatggtac aaaatcattt gttggtactg atgttttaaa
     7081 gctgtgctgt tattatgcta gggcaccatt gtggtgttct agttttttgt tatactgaaa
     7141 taaattttca gagaatgtgg gggaaggcgg taattagatt aattcaagac gtaattcaga
     7201 acttagttgg ccaagctaac gaaatcaccc caatttatca gtttgattgg gaaacttata
     7261 tattggcgac taaaaaatat gaacgtcatt tagaggtgtg tctattagta gaaaattcga
     7321 attgtttttc ggattcaaag agaatgtgtc gataaaagag atatgaaagg ctataattcc
     7381 aaccttatgg ttaaagggca agattgttct acgctttact taattattag tttgacagcg
     7441 ttggttcttt tagtgattgc tggtgtttct ttgtcactaa tacttgcaag cttacaagga
     7501 aagtagtgga gaacctatcc ataatcaact aacagctatg atggatctca cctattaata
     7561 tagttaagga gtgtccgata ctcctttttt gaaatacagt tggaagttac agaaaacata
     7621 ttttcatgtt ataatagtgg agaaagaaat gaggtgctaa tattatggaa caaacctttt
     7681 ttatgataaa acctgatggt gtaaaacgtg gttttattgg agaagttatt agtcgtattg
     7741 agcgtcgagg cttctctatt gatcgcttag aggttagaca tgcagatgca gatattttga
     7801 aaagacatta tgcagaactg acggatagac cattctttcc aacgttagta gattatatga
     7861 ctagtggtcc agtaatcatt ggagttattt cgggagaaga ggtcatttca acttggcgca
     7921 ccatgatggg gagtactaac cccaaagatg cccttccagg aactattcga ggagattttg
     7981 cacaagctcc ctctccaaat caagcgacat gtaatattgt tcatgggtca gactcacctg
     8041 aatctgcaac tcgcgagata gccatttggt tcaataacta atagacttaa tgaagattga
     8101 tgttcaagtg ttaattctct tttacatcgg tcttcttttt gatataatat tctagagtct
     8161 ataggactgt tttaatattt tagaagttaa acatatcaaa ttgtatagga aatagaatga
     8221 atattgaaga tttgaagaaa cgtcaagaaa agattagaaa cttttcgatt atcgctcata
     8281 ttgaccatgg aaaatcaacc ttagcagacc gtattttaga gaaaacagaa acggtttcta
     8341 gtcgtgaaat gcaggcgcag ctgttggact ctatggatct agaacgtgag cgtgggatta
     8401 ctataaagtt aaatgctatt gagcttaatt atactgctaa agatggtgaa acttacattt
     8461 ttcacttgat tgacacgcca gggcatgttg acttcacata tgaagtatca cgttcattag
     8521 cagcttgtga gggtgctata ctggttgttg acgcagctca aggaattgaa gctcaaactc
     8581 tggcaaatgt ttatttggct ctagacaatg atttagagat actaccagtt attaataaaa
     8641 ttgatttacc agcagcagat ccagaacgtg tacgtgcaga agtagaggat gttattggtc
     8701 tagatgcttc agaagctgtg cttgcctcag ctaaggcagg gattggtatt gaagaaattt
     8761 tagagcagat cgttgaaaaa gttcctgctc cgactgggga agtagatgct cctctgcaag
     8821 ctttaatttt tgattctgtt tacgatgctt atcgtggggt aattctgcaa gtaaggattg
     8881 taaatggtat ggttaagcca ggagataaaa ttcaaatgat gtctaatggt aaaaccttcg
     8941 acgttactga ggttggtatt ttcactccca aagcagttgg acgtgatttt ttagcaactg
     9001 gagatgtagg gtatattgct gcctcaatta aaacggttgc tgatacacgt gttggtgata
     9061 ccattaccct tgctaataac ccagcaatag aacctttgca tggctataag caaatgaacc
     9121 cgatggtttt tgccggtctt tatccaattg aatcaaataa gtataacgat ctgcgtgaag
     9181 cccttgaaaa attacaattg aatgatgcta gtctacagtt tgaaccggaa acatcacaag
     9241 cattagggtt tggtttccgt tgtggtttct taggactttt acatatggac gttatccaag
     9301 agcgcttgga acgagagttc aatattgatc ttattatgac tgcaccatca gtagtttatc
     9361 atgttaatac aactgacggt gaaatgcttg aagtttcgaa tccatctgaa tttcctgatc
     9421 ctactcgtgt tgacagcatt gaagaaccat atgttaaggc gcaaattatg gttcctcaag
     9481 aatttgtcgg tgctgtaatg gagttagcac aacgtaaacg tggtgacttt gtaacaatgg
     9541 attatattga tgataatcgt gttaatgtta tttaccaaat tcctttagct gaaattgtgt
     9601 ttgacttctt tgataagctt aaatcatcaa cacgaggcta tgctagtttt gattatgaaa
     9661 tttccgaata tcgtcgctca cagttagtta aaatggatat tcttttaaat ggtgataaag
     9721 ttgatgcttt aagctttatt gttcacaaag aatttgctta tgaacgtggt aaattgatcg
     9781 ttgataagtt gaaaaaaatc attcctcgtc agcagtttga agttcctatc caagcagcta
     9841 ttggacaaaa aattgtggct cgttctgata taaaagcact acgcaaaaat gtcttagcaa
     9901 aatgttatgg tggtgacgtt tcacgtaaac gtaagttgct tgaaaaacaa aaagctggta
     9961 aaaagcgcat gaaagcaatt ggttccgtcg aagttcctca ggaagctttc ttaagtgttt
    10021 tatcaatgga cgatgatgac aaaaaataat tcagaaagat gatatcattt atctatttta
    10081 aagtaactta gcttcgtgct taagttactt tttctttgtc tgcaattatc aaataatatg
    10141 gctttataat atctttgttt aaatataaat gagctataaa tgagtttttg ttagtaacta
    10201 aataacaaaa agatggcaaa tagtattgca aaaagaaaaa catctgatat aattaactaa
    10261 ttaactaaaa cgaaaaaaca tttatggagg actcatgaaa ggtcaaaaaa ttattgctct
    10321 agctggtctt gtcctgtcat gccattttgc tcttacggca tgtcatactc aagaacacaa
    10381 aaattctcat catattaaaa caaagcaggt tgctaagaaa aaagctaata aaaagaaagt
    10441 ctctgttaag gaaagtcata aaaaacgcaa gggtgttgca ggggttgact ttccaactga
    10501 tgatggcttt ttactgacta aagattcgaa gattttatct cataccgatt ctggaatcgt
    10561 tgtagcacat ggaaatcatt ctcattttat tttttactct gatttaaaag gttcaaaatt
    10621 tagctattta attcctaatg caaatgcaaa aactaataaa aagcaagcgg taagaaactt
    10681 taaagcaggg gcggtagctg ttaatacatt aaatgatggc tatgttttta atcccgcaga
    10741 tattgtttca gaagatgcta atggctacgt ggtcagacat ggtgatcatt tccactatat
    10801 tccaaaagca agtttatctc agcaaaagca agtacaagca agtagagctg tttcacgttt
    10861 aggaaatcaa aataatagcc attacagagt taatagttct aaaattgcag ggcttcacca
    10921 cccaacaagt gatggtttct tattcaatgg acaaggaata aaaggcacta ccccaacagg
    10981 tattttagta gaacatcata atcatctgca ttttattagt tttgctgatt taagaaaagg
    11041 gggatgggga tcaattgctg accgttacca accacaaaag aaagttgatt ctaaaaaaca
    11101 gtcaccatca agtaaaaagc caagaactga aaatacttta cctaaggaca tcaaagataa
    11161 gctagcttac cttgctcgtg aattacattt agatatttca cgtatcagag tccttaaaac
    11221 actaaatggt gaaattggat ttgaataccc acatgatgat catacacatg ttattatggc
    11281 caaagatatt gatctaagta agccaattcc aaatccacat catgatgacg aagatcatca
    11341 caaaggtcat caccatgatg agtccgacca taagcatgaa gaacacgagc acactaagtc
    11401 aaataaatta tctgatgaag atcaaaagaa attaatttat ttagcagaga agcttggttt
    11461 aaatcctaat caaattgaag tattaacgtc agaggatggg agtatcatct ttaaatatcc
    11521 acatgatgac cactcacaca ctatagcaag taaggatatt gaaattggga agcctatccc
    11581 agatggacac cacgatcact ctcatgcaaa agataaagtt ggtatggcaa ctctaaaaca
    11641 aattggtttt gatgatgaga ttattcagga tattctgcat gcagatgctc caacaccatt
    11701 cccatccaat gaaactaatc ctgaaaaaat gcgtcagtgg cttgcaactg ttactaagat
    11761 taacattgga caaagaacca atccattcca gcgttttggt ctatcattga tgcctaatat
    11821 tgaagttcta ggaattgggt ttactccgat taatgatatg acgccagttt tgcaattcaa
    11881 aaaacttaaa cagctttgga tgacaaatac tggtattaca gattattctt tccttgataa
    11941 gatgccacta ttagaaggat tggatatttc acaaaacggt attaaggatt tatctttcct
    12001 tacaaaatat aaacaattaa gtcttattgc tgcagctaat aatggcatta cttcgctaaa
    12061 accattggct gaattaccaa accttcaatt cttagttttg agtcataaca atatctctga
    12121 cttgacaccg ttatcaaact tgacaaaact ccaagaattg caccttgatc ataataatgt
    12181 gaagaattta agtgcacttt ctggtaaaaa agatttgaaa gttctagatt tatcaaacaa
    12241 taaatccgca gacttatcga ctctaaaaac aactagtctt gagacgcttc ttttaaatga
    12301 gacgaataca agcaatctaa gtttcttaaa gcaaaatcct aaggtatcta atttaacaat
    12361 taataatgcg aaattagcat cattagatgg tgttgaagag agcgatgaaa ttgttaaagt
    12421 agaagctgaa gggaaccaaa ttaagtcact agtattgaaa aataaacaag gttcgttaaa
    12481 attcttgaat gtgactaata atcagttaac atcacttgaa ggtgttaata attatacttc
    12541 acttgagacc ttaagtgttt ctaaaaataa acttgaatct ttagatatta aaacacctaa
    12601 caaaacagtt acaaatcttg attttagcca taacaatgtt ccaacatcac aattgaaatt
    12661 gaatgaaaaa aatattcctg aagcagtagc taagaatttt ccagcagttg tagaaggttc
    12721 aatggttgga aatggtagtc ttgctgaaaa agcagctatg gcttctaaag aggacaaaca
    12781 agtttcagat aatactaatc accaaaaaaa cactgaaaaa tctgctcaag cgaatgctga
    12841 tagtaaaaaa gaaaacccta aaacacatga tgaacatcat gaccatgaag aaacagatca
    12901 tgcacatgta ggtcatcatc accattaatc ttttacggag ctaatatata aaatccttca
    12961 agaatttctt ggaggatttt gcatttatat gctaaatcat ttattttttt agaaaattag
    13021 ctataatatg actatggtta aaagagaaga tgtgatttta aatgcaactg aaaatatggt
    13081 ttaccataaa ttaaaaaatg atccaagtgg ccatgactgg tttcatattg ttagagtaag
    13141 aaatcttgca gtagaattag cacacaaaga aggtgctaat acctttattt gtcaaatggc
    13201 tgcacttttg catgatatta ttgatgacaa aatctgccaa gatagtaagc aagcgtcgta
    13261 tgaattgact caatggttat atagtcaaga tcttgccata gcagaagtag aacatatcct
    13321 tgatattctt gaaaatattt cattcaaagc aggaactgga ttgactatga aaacattgga
    13381 aggtcaaatt gttcaagatg cagatcgcct agatgctatg ggggctattg gtattgcaag
    13441 aacgatggct tattcaggga gtaaaggacg tttaattcat gaccctaact taaaaccacg
    13501 agaaaacctc actttagaag agtatcgtaa tggtcaggat acagctatca tgcattttta
    13561 cgaaaaatta ttaaagttaa aagaccttat gaatacaaaa caagggaaaa tgcttgctca
    13621 aaaacgtcat gattttttag aactatattt agccgaattt tatgctgaat ggaatggtaa
    13681 aggttaggag tggatatgat tcgtagagcg aaagaaaaag atttgcctgc tattgcagag
    13741 ctattgaaac aaattttagt gttacatcat ggggttcgcc cagatatttt tcatacaaga
    13801 ggaagtaagt tttctaaaga acaattgaaa gaaatgctga tggacgaatc aaggcctatc
    13861 tttgtttatg aatcagacga gggtaaagtt gttgcccact tgtttcttca attacaagaa
    13921 aaacgtgact tacctcgaaa gtcctttaaa actctctata tagatgattt gtgcattgat
    13981 gaggaagtaa gaggtcagca aattggacaa aagttgatgg attttgcaag acaatatgct
    14041 aagaaacatg ggtgttataa tataacttta aatgtttgga atgataatca gagagcggtc
    14101 tctttttatg aaaaattagg ttttaaacct caacaaactc aaatggagga gatactataa
    14161 tgaaagaaac acaagaagaa ttgcgtcagc gtattggaca tacagcttat caagtaaccc
    14221 aaaattcagc tacggaacat gcttttactg ggaagtatga tgattttttt gaagagggta
    14281 tatatgtaga tatcgtcagt ggagaggttt tattctcttc tttagataaa tttcaatctg
    14341 gttgtggttg gccagcattt tcaaaaccaa ttgagaatcg aatggtgaca aatcatcaag
    14401 atcactctca tggaatgcat cgtattgagg ttaggagtcg tcaagctgac tctcatttag
    14461 ggcatgtatt taatgatgga ccggttgatg caggtggttt acgctattgt atcaattcag
    14521 ctgccttaga ttttattcct tatgaccaga tggcaaagag aggctatggg gactacttat
    14581 cactttttga ttaataagag acgacttaag ggtatatctt aagtcgtttt ttatgtatgg
    14641 taaaaagaat tggtggcagg tgtattcttc atcagtttat tttctgaaaa aagagtataa
    14701 tataggtatc aaaaatgaaa acgttttaga gaaggagact agagtatggc ttctacaaat
    14761 aatgtttcag atacaaacac atgttttgct aaagaggagt tagaagagat tttcgaagaa
    14821 ttagggacaa ctcaaggtgg tttatcagat gaagaggttg ctgtacgtca gaaaaaatat
    14881 ggtttaaacc tgttatctga agtagagcaa gaatcaatta ttttattgtt tctgaaaaat
    14941 tttactagtc ttatggcaat actattatgg gttggaggat ttgtggctat tgtttctaat
    15001 agcttagagc taggtcttgc catttggatg gttaatgtga ttaatggtat ttttagtttc
    15061 attcaggaat accgtgcaag ccaagcaaca caagctctca agaaaatgtt accatcttat
    15121 tcacgtgttc ttagaaaaag ctcagaagaa aagatattat cagagcaact agtcccaggc
    15181 gatattgttc ttattgaaga aggagatcgt atttcagcag atggtcgctt gattaaaaca
    15241 acagatttgc aagtaaacca atcagctttg acaggggaat caaatcctat ttataaagat
    15301 agcaatgttg aaaacgacca gtctaaaaca ttgattgaat gtgataatat ggttttcgca
    15361 ggaacgacgg tctccagtgg tagcgcaaca atggttgtta cggcaattgg aatgcaaact
    15421 caatttggtc aaatcgctga tttaacacaa gggatgaaat cagaaaaaag ccctcttcaa
    15481 agagaacttg atcggttgac caaacagatt tctatcattt caattacggt aggaattatc
    15541 tttttcttag cagcgacttt ttttgttaag gagccagtgt caaaatcatt tatctttgcc
    15601 ttggggatga tcgttgcatt tatcccagaa ggtttactac ctaccgttac actttctcta
    15661 gctatggcag tccaacgaat ggctaaagaa cacgcacttg tcaaaaaact ttcttcagtt
    15721 gaaactcttg gagcaacctc agttatctgt tctgataaga ctggtacatt aacgcaaaat
    15781 gaaatgaccg tcaatcattt gtggcaaaat ggaaagagtt accaagtgac aggactgggt
    15841 tatgctccag aaggacagat tctttttgaa ggtgacaata tttgctttgg taatagtgat
    15901 agaggagact tagaaaaact tattcgtttt gctcatctct gtagcaacgc tcaagtatta
    15961 ccaccaaatg atgaacgttc aacttatact gtacttggag atccgacaga agcttgtttg
    16021 aatgttttat tggaaaaatc agggataaac agacaagaaa atcgtaagtt tgcaccacgt
    16081 ttgaaagaat taccttttga ctcggttaga aaacgcatga caactattca tagcttagga
    16141 ggcgatgaaa aggataaaaa aatttcaatc acaaaaggtg cgccgaaaga aatacttgat
    16201 ttatcagatt atgtcttatc tgatggcaaa gttatcccct taaataagga agaacgtaat
    16261 aaaatacagt tggctaatga tacatttgct aaagatggac ttcgcgtctt agctgtttca
    16321 tactgcgata ttgaaggctt ttcaaaagaa caatggacgc aagaaaattt ggaacaacac
    16381 atggtgttca tagggttgat agcaatgagt gatccacctc gtgaaggcgt tcgcgaagct
    16441 attgataaat gtcatgcagc aagcattcgt attattatgg tgactggaga ttacggatta
    16501 acggcactga gtatcgctaa aaatattggt attattcgta acgatggtgc taaagtaatt
    16561 tcaggtttag agttatcaga aatgacggat agccaattga aaaaagaatt gtcaggtgaa
    16621 gccgtttttg ctcgtgtagc accagaacaa aaatatcgag tagtaacgat attacaggag
    16681 atgggtgaag tagtagcagt tacaggagac ggggtcaatg atgcgcctgc gctaaaaaaa
    16741 tccgatattg gtgtagccat gggtgttacc ggtactgatg ttgctaaaga atcagctgat
    16801 atgattctga cagatgatca ttttgcttct attgtacatg ccgtggaaga agggcgggct
    16861 gtttatcaaa acattaagaa attcttgacg tatattttta atagtaatac tcctgaggcg
    16921 gttccgtcgg cattcttcct tttttctaaa ggttttattc ctttaccatt gactgtaatg
    16981 caaatacttg ctgttgattt gggaaccgat atgcttcccg ctttaggtct aggcgtggaa
    17041 ccaccagaaa cagatgttat gaatcgtcct ccaagacggc taacagatcg cttattggac
    17101 aaagggctac ttataaaatc tttcctttgg tacggcacta ttgaatccgt attagctatg
    17161 ggtggttttt tctgggctca ttacttacgt tatggtaatt ttactttttt cgtcgcaaac
    17221 ggcataccgt acagggaagc gacaactatg acacttggag ctattatctt tagccagata
    17281 ggtatggtta tgaatagcag aacgagctat caatctatta aaacactatc tatttttggt
    17341 aataaattaa ttaattttgg tataataatg gagattttag catttttagt cttagtatac
    17401 gtgccactat tccataactt attcaacaca gcaagcctag gtttatcaca ttggctctat
    17461 ttaataggct gcccgtttat catgataggt ttagatgaag ttcggaaatt attcagcagt
    17521 agaaagaata aacggtgatg tcaaatataa taacttattt aaaaaataat tcaaacttaa
    17581 cttttgatga attagcatta aacgatgttg acattctttg tttaaatgaa tttggctata
    17641 tttcttttga aaaacttata aacactacag aaatgaagtc agttttagtc tgtgagttat
    17701 atcatgagta ccttcaaact atggctaaat catatagctt tatgttcacg tcacaacgac
    17761 atgatttatg tcagttaatg atgacctcga aacgttttaa aaacttaact ttatcttatt
    17821 atcgagctga gattagttta gaatttgaaa aacaatttgc agctatggtt tttactattc
    17881 ctaacattaa ttatcatcag gttgtatttc gtggtactga tgccaacctt attggttgga
    17941 aagaagattt taaattgacg tatatgagag aaatatcggc tcatcgttca gcgattaaat
    18001 acttaaatac aatattgcct tattttgata aagtagttct ctcaggtcat tctaagggtg
    18061 gtaatttagc cttgtacgct gcaatgttta caaagccaga tttaaaagca aaaattgatt
    18121 tgatttggct tattgactcc ccaggactac aaaaaacttt gttacctact acagagtata
    18181 aaactactaa acaaaagtgc attcgcttac taccagaaga atctattgtt ggtatgatgc
    18241 tttatagtga tatagagccg ctaattatta gtagcaatgc gcgtggaatt ctgcaacacg
    18301 atgttacaac ttgggaaatt caagaaccag ctattttaaa gactggaaca ggactatcgc
    18361 taaaaagtat ttgttttgaa aagacatttc aacagtggat ggctgaatta aaaagtcaag
    18421 aaagaaaatt attttttgat ttattatttg atagcttcct atcaagtggc gtatctagtt
    18481 tggatgactt caacttggct tccagagcaa aaatgatgaa agcttttcat agctttagag
    18541 aactagatga tgataaaaaa aggttattta acaaatcttt aaaattatta gtaaccatat
    18601 ttttgggagc ttatcatgat
//
LOCUS       DATRTN010000029        18482 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.029, whole genome shotgun sequence.
ACCESSION   DATRTN010000029 DATRTN010000000
VERSION     DATRTN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18482
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.029"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..728
                     /gene="rarD"
                     /locus_tag="VBE22_001028"
     CDS             <1..728
                     /gene="rarD"
                     /locus_tag="VBE22_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEO7202497.1"
                     /translation="SGRKTVYLKDLKGLVNNKKSFWTMFVASILISINWLVYIFAVTH
                     GHATEASLGYYMMPIISILLSVLVLREHLARVVSLAILIAIMGVGILVYQTGHFPLIS
                     LTLALSFGFYGLLKKSISLSSDFSMLVESSFIAPFALIYIVFFAKDFLTDYNILQLVL
                     LSLSGIITAVPLLLFAEAIKRAPLNIIGFIQYINPTIQLLLALFIFKETIVSGEVIGF
                     IFIWLAILVFSIGQVHTMLKKGK"
     gene            730..1992
                     /locus_tag="VBE22_001029"
     CDS             730..1992
                     /locus_tag="VBE22_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEO7202498.1"
                     /translation="MTYQEALEWIHSKLAFGIKPGLERMRWMLEQLGNPQNNLSAIHV
                     VGTNGKGSTTSYLQHIFTNSGYQVGTFTSPYIVDFRERISIDGQMIPESDFIKLVETV
                     RPVVERLHLETNLEPATEFEVITVLMFYYFGNSCPVDIVIIEAGMGGYYDSTNMFKAL
                     AVTCPSIGLDHQEVLGRTYVDIAEQKVGVLKKGVPFVYANDRQDVEEVFQIKAKETHS
                     QTYRLHNDFYIKEEENYFNYIGPQANIDHIQLQMPGHHQVSNASIAITTSLLLRDKYP
                     KLTLQTIKDGLEMTKWVGRTELIFPNVMIDGAHNNESVDALVQVIKKYQQKNVHILFA
                     AINTKPIESMLESLSSIAPVSVTSFDYPKSINLDKYPKAYTRVSDWKKWLHDINLTSD
                     KDFYVITGSLYFISQVRQELLLIKTTTT"
     gene            2011..2574
                     /gene="folE"
                     /locus_tag="VBE22_001030"
     CDS             2011..2574
                     /gene="folE"
                     /locus_tag="VBE22_001030"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEO7202499.1"
                     /translation="MPNQEKMEKAIYQFLEALGENPDREGLKDTPKRVAKMYIEMFSG
                     LNQDPKEQFTAVFSENHEEVVIVKDIPFYSMCEHHLVPFYGKAHIAYLPNDGRVTGLS
                     KLARAVEVASKRPQLQERLTAQVAQALEDALAPKGIFVMIEAEHMCMTMRGIKKPGSK
                     TITTVARGLYKDDRYERQEILSLIQKG"
     gene            2578..3381
                     /gene="folP"
                     /locus_tag="VBE22_001031"
     CDS             2578..3381
                     /gene="folP"
                     /locus_tag="VBE22_001031"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEO7202500.1"
                     /translation="MKIGQYDITGKACIMGILNVTPDSFSDGGSYTTIDSALNQVGEM
                     LEQGVAIVDIGGESTRPGAVFVTSEEEIKRVVPMIKAIREVYPDLLLSIDTYKTEVAQ
                     AALDAGVHILNDVWSGLYDGKMLSLAAERNVPIILMHNQEEAVYQDIKKEVCEFLLER
                     ADHALEAGVSKDNIWIDPGFGFAKTEEQNLELLKGLEQVCDLGYPVLFGISRKRTVNY
                     LLGGNREVIERDMGTAALSAWAIAKGCQIVRVHNVEVNKDIVTVISQLV"
     gene            3383..3745
                     /gene="folB"
                     /locus_tag="VBE22_001032"
     CDS             3383..3745
                     /gene="folB"
                     /locus_tag="VBE22_001032"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEO7202501.1"
                     /translation="MDKIYLNKCRFYGYHGAFSEEQTLGQVFQVDAVLSLDLAKASQT
                     DDLIDTVHYGEVFDCIKNHVENEQYQLIEKLAGVIVEDIFLQFHPVQAITLKITKDNP
                     PINGHYESVGIELERKRA"
     gene            3742..4230
                     /gene="folK"
                     /locus_tag="VBE22_001033"
     CDS             3742..4230
                     /gene="folK"
                     /locus_tag="VBE22_001033"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEO7202502.1"
                     /translation="MTTVYLSLGSNIGDRETFLKQALFSIDHLQKTKVAQISAIYETA
                     AWGNTNQEDFFNICCQVETDLAPFELLDYCQEIEKRLKRVRHEHWGPRTIDIDILLFG
                     NQVINQEDLVVPHPYMTKRAFVLVPLLEIAPQLSLPNGSKLEDYLEKLNLGEVHYLKP
                     VE"
     gene            4374..5276
                     /gene="murB"
                     /locus_tag="VBE22_001034"
     CDS             4374..5276
                     /gene="murB"
                     /locus_tag="VBE22_001034"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEO7202503.1"
                     /translation="MIKTIQKELEGLDIRFDEPLKKYTYTKVGGPADYLAFPRNRLEL
                     SRIVKFANSQNIPWMVLGNASNIIVRDGGIRGFVIMFDKLSTVTVNGYVIEAEAGANL
                     IETTRIARYHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILLSAQVLTPQGELKT
                     IEARNMQFGYRHSVIQESGDIVISAKFALKPGDHLMITQEMDRLTYLRELKQPLEYPS
                     CGSVFKRPPGHFAGQLISEAHLKGQRIGGVEVSQKHAGFMVNIAEGSAQDYENLIEHV
                     INTVESTSGVHLEPEVRIIGESLL"
     gene            5325..6479
                     /locus_tag="VBE22_001035"
     CDS             5325..6479
                     /locus_tag="VBE22_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202504.1"
                     /translation="MTNPIIAFKNVSKVFEDSNTVVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLEASTGDIYLDGKRINDVPTNKRDVHTVFQNYALFPHMTVFENVAF
                     PLKLKKMDKKEIQKRVQETLKMVRLEGFEKRAIQKLSGGQRQRVAIARAIINQPKVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILSGKMIEDYLVEFNGKRFEAVDGGMRPNESVQV
                     VIRPEDLQITLPDEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIEGEVIG
                     LDFTPEDIHIMRLNETEEEFDARIEEYVDTDDHEDGLINAIEEERNEENL"
     gene            6463..7257
                     /locus_tag="VBE22_001036"
     CDS             6463..7257
                     /locus_tag="VBE22_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7202505.1"
                     /translation="MKKTSSLFSIPYMAWLFLFVLAPVALIAWNSFFDINGHFTLANY
                     QTFFSSGTYLKMSFNSVLYAGIVSFITLLISYPAAYLLTKLKHKQLWLMLVILPTWIN
                     LLLKAYAFMGIFGQQGGINAFLTFIGIGPKQILFTDFSFLFVAAYIELPFMLLPIFNA
                     LDDIDQNLIYASDDLGANAWQTFQKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLITQNKGMGSTIGVILILVMVAIMWLTKERKA"
     gene            7254..8030
                     /locus_tag="VBE22_001037"
     CDS             7254..8030
                     /locus_tag="VBE22_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7202506.1"
                     /translation="MKKFANIYLALVFIILYIPIIYLIFYSFNKGGDMNSFTGFTFSH
                     YGELFQDSRLMLILVQTFFLAFLSALLATIIGTFGAIWIYQVRRRHQTSILSLNNILL
                     VAPDVMIGASFLLVFTVIGLQLGFTSVLLSHVAFSIPIVVLMVLPRLKEMNDDMINAS
                     YDLGASTWQMLKEVMLPYLSSGIISGFFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARRGISLEINALSTIVFLFSILLVIGYYYISKEKGEKNA"
     gene            8023..9096
                     /locus_tag="VBE22_001038"
     CDS             8023..9096
                     /locus_tag="VBE22_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7202507.1"
                     /translation="MRRVYSFLGGIVLVILILFGLTTYLEKKSSSTPNSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMHTKIKQGGTTYDIAVPSDYMIDKMIKEN
                     LLVKLDHSKIANWDAIGARFKNLSFDPKNKYSIPYFWGTVGIVYNDQLVKTPPKHWDD
                     LWRPEFRNKIMLVDSAREVIGVGLNSLGYGLNTKNISELKAASKKLDALTPNVKAIVA
                     DEMKGYMIQGDAAIGVTFSGEAREMLDGNKHLHYVVPSEGSNLWFDNIVIPKTVKHRK
                     EAYAFINFMMEPKNAAQNAEYIGYATPNLKAKALLPADIKNDKAFYPPDKTIDHLEVY
                     NNLGQKWLGIYNDLYLQFKMYRK"
     gene            9205..10737
                     /locus_tag="VBE22_001039"
     CDS             9205..10737
                     /locus_tag="VBE22_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEO7202508.1"
                     /translation="MENHKNEFQFSLESILGFVWRGIVVGLIAGFVVSIFRLAIEKIF
                     LVVMELYKSAHYQPIILLSITVTSIIAAVIIGFFIKSDPDIKGSGIPHVEGELKGMLS
                     PDWFSIVWKKFIAGILAISSGLMLGREGPSIQLGAMTGKGIAQYLNASRMEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLVWITALVASLVANFVSLNIFGLTPVLA
                     LPSELPSLNLNFYWIFLLMGLFLGILGFIYEWVILRFHVIYDYLGKLFHLPSHLYGIL
                     AVIFILPIGYYFPQLLGGGNGLIVSLPRSNLSLMMLGLFFLIRFLWSMLSYSSGLPGG
                     IFLPILALGSLAGAFFAVGMQYFGIISHQQISLFVVLGMAGYFGAISKAPLTAMILVT
                     EMVGDLKQLMAIGIVTMVSYIVMDLLKGEPIYEAMLAKMTFNPKDKVMTPTLIELTVS
                     DKISGKYVRDLELPENVLITTQIHHKTSAVVSGNTILNAGDTIFLVVNESEIKEVREQ
                     LM"
     gene            complement(10794..11723)
                     /locus_tag="VBE22_001040"
     CDS             complement(10794..11723)
                     /locus_tag="VBE22_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO7202509.1"
                     /translation="MNSLDNLLSHNLEDNRHLLPYEHMHTEVRNGYPDILFHWHPELE
                     ISYVHEGTARYHIDYDFFNSQSGDIILIRPNGMHSIHPIENKEHITDSIKFHLDLIGY
                     SIVDQVSLRYLQPLQTSSFKFIQCIKPSMTGYNDIKNCLFDIFNISKEENRHFELLLK
                     AKLNELLYLLYYHQYVIKKHTDDTYRKNERIRDLIDYINNNYQQNLTIEFLADYMGYS
                     KTHFMTVFKQHTGTSCTEFIIQVRLNKASEHLINSTTAIIDIANSVGFNNLSNFNRQF
                     KRYYHTTPRQYRKQFTNKKDGSTLVYRNINTKK"
     gene            12347..13168
                     /locus_tag="VBE22_001041"
     CDS             12347..13168
                     /locus_tag="VBE22_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO7202510.1"
                     /translation="MIKLIATDMDGTFLRSDKTYDKARFSSLLTLMEKYDIKFVAASG
                     NLYDQLLLNFLEYPNRIAYVAENGGRVIDQDGTLLKETYLSNDTVAAVLSYLYQNYPE
                     TLISLSGEKRSYLERRTPINRRTELEYYMPNFIYKDHLLPLDDDRYFQMTLWVNENLV
                     SEMLLDISEHFKNHHIRLTSSGFGCIDVLPADVNKADGIAILLEKWGLKQDQVMVFGD
                     GGNDVEMLRAANISYAMSNAPEEIKAIAKYQTVSNDQDGVLETIENFLHKMSDNE"
     gene            13269..13883
                     /locus_tag="VBE22_001042"
     CDS             13269..13883
                     /locus_tag="VBE22_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEO7202511.1"
                     /translation="MLEVIDKALRDYQMKREQFFEINNQTVQEGAIVFTGDSIVEFFP
                     LKKHLGRDYPLVNRGVAGSDTYWLLENLRTQVWELLPSKVFILIGTNDIGLGHSQSEI
                     IANITDIIAEIRAESYMTEINILSVLPVSEEDDYIERVKVRNNQAIKALNKTLSVISG
                     INYIELYDLLVDEKGQLASSFTKDGLHLTDQAYAKISETIKLHL"
     gene            14043..15482
                     /locus_tag="VBE22_001043"
     CDS             14043..15482
                     /locus_tag="VBE22_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEO7202512.1"
                     /translation="MMVKQVFPKGFLWGGATAANQCEGAYNVDGRGLANVDVVPTGED
                     RFAIISGQKKMFDFEEGYFYPAKESIDFYHHYKEDLALLAEMGFKTYRMSIAWTRIFP
                     KGDELYPNKAGLRFYENIFKECRKYGIEPLVTITHFDCPIYLIKHYGGWRSRKMIGFY
                     ERLVRALFTRFKGLVKYWLTFNEINMILHAPFMGAGLYFEDGENQEQIKYQAAHHELV
                     ASAIAVKIAHEVDPNNQIGCMLAAGQYYPNTCHPQDYWASMQKNRENYFFIDVQARGK
                     YPNYAKKYFEHLGISIQMTAEDLALLRDYTVDFISFSYYSSRVASGNPTVSEQVQENI
                     FASLKNPYLKSSEWGWQIDPLGLRITLNAIWDRYQKPMFIVENGLGAVDIPDENGYVE
                     DDYRIDYLRQHIAAMRDAIYVDGVNLIGYTTWGCIDLVSAGTGEMEKRYGFIYVDRNN
                     KGEGTLKRYKKKSFYWYKKVIASNGSQIE"
     gene            15573..16706
                     /locus_tag="VBE22_001044"
     CDS             15573..16706
                     /locus_tag="VBE22_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO7202513.1"
                     /translation="MKFEKRQVYYVVITFAICYAIQAYWGAVSNILTTLHKAIFPFLM
                     GAGIAYIINIVMSVYERLYIKLFKGSRLLMAIKRSVSMILSYATFIGLIVWLFSIVIP
                     DLISSLSSLLVIDTGALAKLVNNLNENKQISEALNYMGTDKDLVSTLSGYSQQILKQV
                     LSVLTNLLTSVSSIAATLLNVFVSFIFSIYVLANKEQLGRQFNLLIDTYLGSTGKTFH
                     YVRHILHQRFHGFFVSQTLEAMILGSLTVIGMLIFQFPYALTVGVLVAFTALIPVVGA
                     YIGVTIGFILIATESLTEAFLFVLFLILLQQFEGNVIYPKVVGGSIGLPSMWVLMAIT
                     IGGALWGILGMLLAVPVAATIYQIVKDHIIKRQTLRNRARTYR"
     gene            16802..17482
                     /gene="radC"
                     /locus_tag="VBE22_001045"
     CDS             16802..17482
                     /gene="radC"
                     /locus_tag="VBE22_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEO7202514.1"
                     /translation="MYHIELKKEALLPRERLVDLGADRLSNQELLAILLRTGIKEKPV
                     LEISTQILENISSLADFGQLSLQELQSIKGIGQVKSVEIKAMLELAKRIHKAEYDRKE
                     QILSSEQLARKMMLELGDKKQEHLVAIYMDTQNRIIEQRTIFIGTVRRSVAEPREILH
                     YACKNMATSLIIIHNHPSGSPKPSESDLSFTKKIKRSCDHLGIVCLDHIIVGKNKYYS
                     FREEADIL"
     gene            complement(17495..18133)
                     /locus_tag="VBE22_001046"
     CDS             complement(17495..18133)
                     /locus_tag="VBE22_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEO7202515.1"
                     /translation="MIMDKSIPKATAKRLSLYYRIFKRFNTDGIEKASSKQIADALGI
                     DSATVRRDFSYFGELGRRGFGYDVKKLMNFFAEILNDHSTTNVMLVGCGNIGRALLHY
                     RFHDRNKMQISMAFDLDSNDLVGKTTEDGIPVYGISTINDHLIDSDIETAILTVPSTE
                     AQEVADILVKAGIKGILSFSPVHLTLPKDIIVQYVDLTSELQTLLYFMNQQQ"
     gene            complement(18288..>18482)
                     /locus_tag="VBE22_001047"
     CDS             complement(18288..>18482)
                     /locus_tag="VBE22_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7202516.1"
                     /translation="SGDGFPLKITINKELSGFKLLITDKSGLRIVNIFKSTDHKILQD
                     KFYFLMDSLVERDIFTKTAK"
BASE COUNT         5944 a         2739 c         3558 g         6241 t
ORIGIN      
        1 tttcgggtag aaaaacagtc tatttaaaag acttaaaagg cttagttaat aataaaaagt
       61 cattttggac aatgtttgta gccagtattt taatttcaat taattggttg gtctatattt
      121 ttgcagtcac gcatggtcac gctactgagg caagtcttgg ttattatatg atgccgatta
      181 tttcaatttt gttgtcggtc cttgttttac gtgaacacct ggcaagagta gttagccttg
      241 caattttgat tgcaatcatg ggagttggaa ttttagttta tcaaactggt cattttcctt
      301 taatttcact aactctagcg ctttcttttg gtttttatgg gttattaaaa aaatcaattt
      361 ctttatctag cgatttttcg atgctggtag aaagtagttt tattgctccg tttgcactca
      421 tttatatagt gtttttcgcc aaagattttt taactgatta taatatatta caattagttt
      481 tactctcact ttcaggaatc attacagcag ttcctctttt attatttgca gaagccatta
      541 agagggctcc tctaaatatt atcggtttta tccaatacat caatccaacc atccaattat
      601 tgctagcatt gtttattttt aaagaaacta tcgttagtgg ggaggtaata ggttttattt
      661 ttatctggct tgctatttta gtttttagta ttggacaagt acatacgatg ttaaagaaag
      721 gtaaataaga tgacttatca agaagcgcta gaatggattc atagcaaatt agcttttggt
      781 attaaaccag gtttggaacg tatgagatgg atgctagagc agttaggaaa ccctcaaaac
      841 aacctatctg ccattcatgt tgttggaacg aatggtaaag gatcaacaac tagttattta
      901 caacatattt ttacaaattc tggttatcaa gttggaacat ttacatcacc atatattgtt
      961 gactttagag agcgtattag cattgatggt caaatgattc cagagtctga ttttatcaaa
     1021 cttgttgaaa ctgttcgtcc agttgttgaa cggttgcacc ttgagaccaa cctagaacca
     1081 gctacagaat ttgaagttat aacagttttg atgttctatt attttggcaa tagctgtcca
     1141 gttgatatcg tcattattga ggctggaatg ggcggttatt atgattctac caacatgttc
     1201 aaggctttag cggttacttg tccgtcaatt ggattagatc atcaggaggt tttaggacga
     1261 acttatgtag atatagctga acaaaaagta ggtgttttaa aaaaaggagt tccattcgta
     1321 tatgctaatg atcgtcagga tgttgaagaa gtctttcaaa taaaagccaa ggaaacccac
     1381 agccagactt atcgtttaca caatgacttt tatataaaag aagaggaaaa ttattttaac
     1441 tatataggtc ctcaagcaaa tattgatcat attcagttgc aaatgccggg acatcaccaa
     1501 gtttccaatg ctagcatagc tataactacc agtttattat taagagacaa atatccaaag
     1561 ttaaccctac agaccattaa agatgggctt gaaatgacaa aatgggtagg ccgtacagaa
     1621 ttaatctttc caaatgtaat gattgatggc gctcataata atgaaagtgt cgatgctctc
     1681 gttcaagtta taaaaaaata tcagcagaaa aacgtgcata ttctatttgc tgcaattaat
     1741 actaaaccga tagagagtat gttagaatca ttgtctagta tcgctccggt ttcggttact
     1801 agctttgatt accctaaatc aataaactta gacaaatatc ctaaagcgta cactagagtt
     1861 agtgattgga agaaatggct acatgatata aatctgacat cagataaaga tttctatgtg
     1921 attactggct ccctttattt tatttctcaa gttcgtcagg aactactatt aatcaaaact
     1981 acaacaactt aaaaaggata ggataaatta atgcccaatc aagaaaaaat ggaaaaagct
     2041 atttatcaat ttttagaggc tttaggagaa aatcctgatc gagaaggatt gaaagataca
     2101 ccgaagcgtg tggcaaaaat gtatatagaa atgttctcag gacttaatca agatccaaag
     2161 gagcaattca ctgcagtctt ttctgaaaat catgaagagg ttgtgattgt caaggatatt
     2221 ccattctatt ccatgtgtga acatcatttg gttccttttt atggtaaagc tcatattgct
     2281 tatttaccaa acgatggacg tgttactgga cttagtaagc ttgcacgtgc agttgaagtg
     2341 gcgagtaagc gtcctcaact tcaggaacgt ttaacagcac aagttgcaca agctttagag
     2401 gatgctttgg cgcctaaagg tatttttgtt atgatagaag cagagcatat gtgtatgaca
     2461 atgcgtggca ttaaaaaacc aggaagtaag acgattacaa cggtagctcg tggtttatac
     2521 aaggacgatc gatatgaaag acaagaaatt ctatcgctaa tacaaaaagg ataaaagatg
     2581 aaaataggac aatatgatat tacaggtaaa gcttgtatca tggggatttt aaatgtaacg
     2641 cctgattcct tctcagatgg tggttcttat accacaattg attcagcttt aaatcaagtt
     2701 ggtgagatgc ttgaacaagg ggtggcaatt gtagatattg ggggagaatc aacgcggcca
     2761 ggtgcagtat ttgtgacttc tgaagaagaa attaaacgtg tagttccaat gattaaagct
     2821 attagagagg tctaccctga tcttttgtta tcaatagata cttataaaac agaggtagct
     2881 caagcagccc ttgatgcagg tgtacatatt ttaaatgacg tttggtcagg tttatatgat
     2941 ggtaaaatgc tatctttagc agcagaacga aatgttccaa ttattttgat gcataaccag
     3001 gaagaagctg tttatcaaga tatcaaaaaa gaagtttgtg aattcttact tgaaagagca
     3061 gatcatgctt tagaagcagg tgtttcaaag gataatattt ggatagatcc tggctttggt
     3121 tttgctaaaa cagaggagca aaatttagaa ctattaaaag gtttggaaca agtgtgtgat
     3181 ttaggttatc ctgtgttatt tgggatctct cgaaaacgta ctgttaatta tcttctaggt
     3241 ggtaataggg aagtaattga acgtgatatg gggactgctg ctttatcggc atgggcaata
     3301 gcaaaggggt gtcagattgt acgtgttcac aatgttgaag taaataagga cattgtgaca
     3361 gtaattagtc agttggtgta atatggataa gatttattta aataaatgtc ggttttatgg
     3421 ttatcatggt gctttttcgg aagaacaaac attggggcaa gtttttcaag ttgatgctgt
     3481 tctatcactt gatttggcaa aagcatcaca aactgatgat ttaattgata cggttcatta
     3541 tggtgaagtt tttgactgta ttaaaaatca tgttgaaaac gaacagtatc aattaataga
     3601 gaaattagca ggtgttattg ttgaagatat tttcttacag tttcatccag tacaagctat
     3661 tacattaaag attacaaaag acaatccacc aattaatggg cactatgaat cagttggaat
     3721 tgaattggag cgcaagcgag catgacaacc gtttacttga gtttaggtag taatattggt
     3781 gatagagaaa cttttctgaa gcaggctctc ttttcaattg atcatttgca aaagacaaaa
     3841 gtcgcgcaaa tttccgctat ttatgaaaca gctgcttggg ggaatactaa ccaagaagat
     3901 ttttttaata tatgttgtca ggtagagaca gacttagcac cttttgaatt attggattat
     3961 tgtcaagaaa ttgaaaaacg cttaaagcgc gtgagacatg agcactgggg tccaaggaca
     4021 atagatattg atatcttact ttttggtaat caagtgatta accaagaaga tttagtagta
     4081 ccccatccgt atatgacgaa gcgagcattt gttctagttc cgttactcga aatagcacct
     4141 caactaagtt tacctaatgg ttctaagcta gaagactatc tcgaaaaatt gaatttagga
     4201 gaagttcatt atttaaaacc agtcgaatag tcagttagga ttttaaaatt aaaatacaca
     4261 gaaattgtga taatgtatcg tcaacaacct atatttttgg tataatagaa atcggtttta
     4321 tgccgatttt ttgtgtctat aaaacacaat aaaacagaat tgagataaag gttatgatta
     4381 aaacaataca aaaagaatta gaaggtctcg atattcgctt tgatgagcct ttaaagaaat
     4441 atacttatac taaagtagga ggacctgctg attatcttgc attcccaagg aatcgccttg
     4501 aactatcgcg tattgttaag tttgctaaca gtcaaaatat tccttggatg gttttaggaa
     4561 atgcgagtaa tattatcgtt agagatggtg gtatccgtgg ttttgttatc atgtttgata
     4621 aactgtcaac tgttacagtc aacggttatg ttattgaagc agaagcagga gctaatctca
     4681 ttgaaacaac tcgtattgca cgttatcata gcttaactgg ctttgaattt gcttgcggaa
     4741 ttcctggaag tgtaggtgga gcagtcttta tgaatgctgg cgcttacggt ggtgaaattg
     4801 ctcatatttt attgtctgca caagttttga caccacaggg tgaacttaaa acgattgaag
     4861 ctaggaatat gcaatttggt tatcgtcatt ctgtcattca agagagtgga gatattgtta
     4921 tatcagctaa gtttgcctta aaaccaggag atcatctcat gattactcag gaaatggatc
     4981 gtttaaccta tctccgtgag ctgaaacaac ctttagaata cccttcttgt ggttctgtgt
     5041 tcaaacgtcc cccaggacat tttgcagggc aactcattag cgaagcacat cttaaaggtc
     5101 aacgtattgg tggagtagag gttagccaaa aacatgctgg ttttatggtt aacattgcag
     5161 aaggttcagc tcaagattat gaaaatctta ttgaacatgt tattaacacc gttgaatcaa
     5221 cttcaggtgt ccatttggaa ccagaagttc gtattatcgg tgagtcgcta ttataaagta
     5281 gtttagtact tactttagtc agaaaaatgg aggatttatg agaattgact aacccaatca
     5341 ttgcatttaa aaatgtatca aaggtatttg aagacagcaa caccgttgtc ttaaaagata
     5401 ttaattttga acttgaagaa ggtaaatttt acaccttatt aggggcctca ggaagtggta
     5461 aatcaactat cttaaatatt attgcaggtt tgctagaggc gagtacaggc gatatttact
     5521 tagatgggaa acgcatcaat gatgttccca ctaataaacg ggatgttcat accgttttcc
     5581 aaaactatgc tttgtttcca catatgacgg tttttgaaaa cgttgctttt ccattaaagt
     5641 tgaaaaaaat ggataagaaa gaaattcaaa aacgtgttca agaaactcta aaaatggttc
     5701 gtttagaagg gtttgaaaaa cgagccattc aaaaattatc aggaggacaa cgccagcgtg
     5761 ttgcgatagc gcgtgctatc atcaatcaac ctaaagtagt cttactagat gaacccttat
     5821 cagcattgga tttgaaattg cgtacagaga tgcaatatga attgcgcgaa ctgcaacaac
     5881 gtttaggcat tacctttgtt tttgtcacac atgatcaaga agaagcgttg gcaatgagtg
     5941 attggatttt cgtcatgaat gaaggtgaaa ttgttcaatc aggaacacca gtagatatct
     6001 acgatgaacc tatcaatcat tttgtagcaa cttttatagg tgaatcaaat atcttatctg
     6061 gcaagatgat tgaggattat ttggttgaat ttaacggtaa gcgctttgag gctgttgatg
     6121 gcggtatgcg ccctaatgaa tcagtccaag tagttattcg tccagaagat cttcaaatta
     6181 ccctacctga tgaaggaaaa cttcaagtta aagtggacac acaacttttt cgaggtgtcc
     6241 actatgaaat tattgcttat gatgatttag gtaatgaatg gatgattcat tcaactcgta
     6301 aagctattga gggggaagtt attggtttag atttcactcc agaagatata catattatgc
     6361 gtcttaatga aacagaagaa gaatttgatg cgcgcattga agaatatgta gacactgacg
     6421 atcatgagga tggtttaatc aatgctatcg aggaggagag aaatgaagaa aacctctagt
     6481 ctattttcaa ttccttatat ggcttggtta ttcttatttg tattagcacc tgttgctttg
     6541 attgcttgga actcgttctt tgatattaat gggcacttta ctttagctaa ttaccagacg
     6601 ttttttagct caggaaccta tcttaaaatg agctttaact cggttctata tgcaggcatc
     6661 gttagtttca taacgctttt gattagctat ccagctgctt atttgcttac aaaattaaaa
     6721 cacaagcaat tatggctaat gctagttatt ttgccaacat ggattaatct tttgttaaaa
     6781 gcctatgctt ttatggggat atttggtcaa caagggggaa ttaacgcctt cttaaccttt
     6841 ataggtatcg gcccaaaaca gattctattt acagattttt catttctttt tgtagcagct
     6901 tacattgaat taccgtttat gttattaccc atttttaatg ccttggatga tattgatcaa
     6961 aatctcattt atgcaagtga tgacttgggt gcaaatgcat ggcaaacttt tcagaaggtt
     7021 atatttcctc tatctctgaa tggtgtaaga gcaggtgtac aatctgtctt tattcctagt
     7081 ttaagtctct ttatgcttac gcgtcttatt ggtggtaatc gagttattac tttagggaca
     7141 gccatagaac aacatttttt aatcacacaa aataaaggta tgggctcaac cataggggtt
     7201 attttaattt tagtgatggt tgctattatg tggttaacaa aggagaggaa agcatgaaaa
     7261 aattcgcaaa tatctattta gcacttgttt ttattatcct ttatattcct attatttatt
     7321 tgatttttta ttctttcaat aaaggtgggg atatgaattc ttttactgga ttcaccttta
     7381 gtcactatgg agaactgttt caggatagcc gcttaatgct aattttagtg caaacattct
     7441 ttttggcttt tcttagcgcc ttattagcta caattattgg tacctttgga gctatttgga
     7501 tttatcaggt aagacgtaga catcaaacct ctattttatc gcttaataat atccttttag
     7561 tagcaccaga tgttatgatt ggtgcaagtt ttctcttagt atttactgtt attggtttac
     7621 agttaggttt cacttctgta ttattgagcc atgttgcatt ctctattcct attgttgttt
     7681 taatggtctt acctcgttta aaagaaatga atgatgatat gattaatgct tcctatgatt
     7741 taggagcttc aacttggcaa atgcttaaag aagttatgtt accctatttg tcgtcgggta
     7801 ttatttcagg tttctttatg gctttcactt actcgctaga tgattttgcg gttactttct
     7861 ttgttacagg taatggcttt tcaacgcttt cagtagaaat ctattcacgc gcacgtcgcg
     7921 gtatatcatt ggaaattaat gccttgtcaa ctattgtctt tttatttagt attttattgg
     7981 taattggtta ttactatatt tcgaaagaga agggagaaaa gaatgcgtag agtttattcc
     8041 tttttaggcg gtattgttct tgttatcctg atcctctttg gtttaaccac ttacttagaa
     8101 aaaaagagta gttcaacacc taactcagat aagttagtta tttataactg gggagactat
     8161 attgatccag ccttattaaa aaaatttaca aaagaaactg gaattgaagt acaatacgaa
     8221 acgtttgact ctaatgaggc tatgcacacc aaaattaagc agggtgggac gacatatgat
     8281 attgctgtac ctagtgatta tatgattgac aaaatgataa aagaaaacct tctggttaaa
     8341 cttgatcatt caaagatagc taattgggat gctattggag ctaggtttaa aaatttgagt
     8401 ttcgatccta aaaataaata ttcgattcct tatttctggg gaactgttgg gattgtttat
     8461 aatgatcaat tagttaaaac tcctcctaaa cattgggatg atttatggcg tccagagttt
     8521 cgaaataaaa tcatgctagt tgatagtgca cgtgaggtta ttggagttgg tttaaattcc
     8581 ttgggatatg gattgaatac aaagaacata tcagaattaa aagccgcttc taaaaagtta
     8641 gatgctctaa caccaaacgt taaggctatt gttgctgatg agatgaaagg ctatatgatt
     8701 cagggagatg cagcgattgg tgttacattc tctggagaag cacgagaaat gcttgatggg
     8761 aacaagcatt tgcattacgt tgttcctagt gaaggatcta acctttggtt tgataatatt
     8821 gtaatcccca aaacagtaaa gcatcgcaaa gaagcttatg cctttattaa tttcatgatg
     8881 gaacctaaaa atgcggcaca aaatgcagaa tatattggtt atgcgacccc taatctcaaa
     8941 gcaaaagcgc tgttaccagc agatatcaaa aatgataaag cattttatcc tcctgataaa
     9001 acaattgatc atttagaagt ttacaataac ttgggtcaaa aatggcttgg tatttacaat
     9061 gatttgtact tacaatttaa aatgtatcgt aagtaatcta ttaacaaaaa gtacttctct
     9121 gatgagaggt atttttagtt accaagagtt cctagaaagt ttgtgatatt tttgttaaaa
     9181 taattgtaag aaaaggagga aaatatggaa aatcataaaa atgaatttca gttttcactt
     9241 gaatcaattc tgggatttgt ttggagagga atagtagtag gcttaatagc aggatttgtt
     9301 gtcagcatat ttcgattagc gattgaaaaa atttttttag ttgttatgga actttataaa
     9361 agtgcccatt atcaaccaat tattttatta agcattacgg ttactagtat tatcgctgca
     9421 gtgataatag gtttttttat caaatctgat cctgatatta aaggttcagg tatcccgcat
     9481 gttgaaggtg aacttaaagg aatgctatcg ccagactggt ttagtattgt atggaagaaa
     9541 ttcattgcgg gtattttagc tatttcttcg ggacttatgc ttggtcgcga aggtcccagt
     9601 attcagttag gagctatgac aggtaaggga attgcacagt atctcaatgc tagtcgaatg
     9661 gaaaaacgtg ttttaattgc tagtggtgcg gcagcaggtc tttcagcagc cttcaacgct
     9721 cctattgcag gccttttatt tgtagttgaa gaaatttacc atcatttttc tcgcttagtt
     9781 tggataacag ctttggtagc aagtttagtg gcaaactttg tctcacttaa tatctttggt
     9841 ttaaccccag ttttagcttt accatcagaa ttaccatctt taaatctcaa cttttattgg
     9901 atttttttac tcatgggtct ctttttgggt atcttaggtt ttatctatga gtgggtaatt
     9961 ttaagatttc atgtgatcta tgattatttg ggcaaactat ttcatctacc atctcaccta
    10021 tatggtatat tagcggtaat ttttattctt cctattggtt actatttccc acaattattg
    10081 gggggtggaa atggcttaat tgtatcactg cctaggagta atcttagtct gatgatgtta
    10141 ggattatttt tcctcattcg ttttttatgg agtatgttga gttacagtag tggcttgcca
    10201 ggaggaattt ttttaccaat tctagcttta ggatctttag caggtgcttt ttttgcagta
    10261 ggtatgcagt actttggtat aatatctcat cagcagatat ctttatttgt tgttctagga
    10321 atggcgggct attttggtgc aatctcaaaa gcaccactaa cagctatgat tttggttaca
    10381 gaaatggttg gcgatttaaa acaattaatg gctattggaa ttgtaacgat ggtatcttat
    10441 attgtcatgg atttattaaa gggagaacct atttatgaag cgatgttggc taaaatgacc
    10501 ttcaatccaa aagataaagt aatgacacct acattgattg aattaactgt ttcagataaa
    10561 atatcaggaa agtatgtccg agatttagaa ttaccagaaa atgtgttaat aacgactcaa
    10621 attcatcata agacatcggc tgtagtatca ggaaatacta tcttgaatgc tggcgacacc
    10681 atttttttag ttgtgaatga atcggaaatt aaagaagtac gtgaacagct gatgtaaact
    10741 aattaacgct taaataataa aaaatagaaa ctagtcatgg atttaactag tttctatttt
    10801 tttgtattta tatttctata gacaagcgta ctaccgtctt ttttgttggt aaattgtttg
    10861 cggtactggc gaggagttgt atgataatag cgtttaaatt ggcgattaaa atttgataga
    10921 ttattaaaac caacgctgtt ggcaatatca ataatggccg tagtggagtt aataagatgc
    10981 tcactagctt tattaagacg aacttgtatg ataaattctg tacaggatgt tccagtatgc
    11041 tgtttaaata cggtcataaa atgtgtctta ctatacccca tataatcagc taaaaattca
    11101 atggtaagat tttgctggta attattatta atataatcaa tcagatcacg aattctctca
    11161 ttttttctgt aggtatcatc agtatgtttt ttaatgacat attgatgata atagagtaaa
    11221 tacaagagtt cgtttaattt agcttttagg agtagctcaa aatgtcgatt ttcttctttt
    11281 gaaatattaa aaatatcaaa caaacaattt ttgatatcat tatatcctgt catactaggt
    11341 ttgatacatt ggataaactt aaaagagcta gtctgcaacg gttgtaaata acgaaggcta
    11401 acttggtcta caatagaata tcctattaaa tctaagtgaa atttaatgct atctgtgata
    11461 tgctctttat tttcaatcgg atgaatagaa tgcataccat ttggacggat caagataata
    11521 tcaccagatt ggctattaaa aaaatcataa tcgatgtgat aacgtgcggt cccctcgtgg
    11581 acatatgaaa tttcaagttc cggatgccag tgaaataaaa tgtcagggta accatttcta
    11641 acctcagtat gcatgtgttc gtagggtaat aaatgccggt tatcttctaa attatgtgat
    11701 agtaaattat caagtgagtt catcgttttt ctttctaaaa tcgtaggata gtattattat
    11761 aacagaaaac tatcaaaaaa tgggtttcgt aggatagtat tattttttga aaggatagtg
    11821 taagttttgt ataggaacat gacatataat ataagtcata gagaaaacgc tttaaaattt
    11881 ttgcttagag tgtttctaat aacttcaaaa tccttacagg gtcgtttaat ctccttatct
    11941 catgtaagga ttttttgttg tattaatatg accaaattta ttaatatact caattattta
    12001 ttttttcaaa acagaataac tatgtgaaaa aaataatttt aggtctctca aaaacacgta
    12061 tagcaatttc gaatctcaaa tattttatta tgactattgc aatagttacg aatctattat
    12121 tcaactcaac aataagaaaa ctcttcagta aatcattatg ttaaacaagc tctgatagta
    12181 tggcgataag tatgggtaaa agtcaattta atctctgaaa caaaatttaa cttaattaat
    12241 agaaccgttg acgtatcagg tatcaaatct ttttggttta tatgtgatat aatgaaagaa
    12301 catattgaga tatttggcac cctcttgaaa ggcaaaggtt acttgcatga ttaaactaat
    12361 agcaacagat atggatggaa cttttctacg ctcagataaa acttatgata aagcacggtt
    12421 ttcttcttta ttaacgttga tggagaagta tgatattaaa tttgtagctg ctagtggtaa
    12481 tttatacgat caattgttgt taaacttttt ggaataccct aatcgtatcg cctacgtcgc
    12541 tgaaaatggt ggtcgtgtca ttgaccaaga tggcacatta ctaaaagaaa cttatttatc
    12601 taatgataca gttgcagctg ttttatccta tttatatcag aactatcctg agacactcat
    12661 cagcttatct ggtgaaaaac gttcatacct tgagcgaaga actcctatta ataggcggac
    12721 tgaattagaa tactatatgc ctaattttat ctataaagac catttgctgc ccttagatga
    12781 tgaccgatat tttcaaatga cactttgggt aaatgaaaat cttgtttctg agatgttgtt
    12841 agatattagt gaacacttta aaaatcatca tatcagattg acctctagtg gttttggttg
    12901 tatcgatgtc ttaccagctg atgtcaataa agcggatggt atcgctattc tccttgaaaa
    12961 gtggggattg aaacaggatc aagtgatggt ttttggtgat gggggcaatg atgtggaaat
    13021 gttgagagca gcaaatattt cttacgctat gtcaaatgcg ccagaagaaa ttaaagctat
    13081 tgctaaatat caaacagtta gtaacgatca agatggtgtg ttagaaacga tagagaactt
    13141 tcttcacaag atgagtgata atgaatagaa ccacgtagct tttaacgaag catactattc
    13201 aattctcagg tgaaattttg ttataataca agaaagccta gagaaatatt taagaatgaa
    13261 ggagaacgat gttagaagtt attgacaaag ctttaagaga ttatcaaatg aaacgagaac
    13321 agttttttga gattaacaat cagacagtac aagaaggtgc cattgttttc acaggagatt
    13381 ctatcgttga gttttttcct ttaaaaaaac atttaggacg tgactatcct ttagttaata
    13441 gaggagtagc aggatcagat acttattggc ttttagaaaa cttgagaacc caagtatggg
    13501 aactattacc atccaaagta tttattttaa ttggaacaaa tgatattggt ttagggcatt
    13561 cacagtcaga aatcatagct aatatcacgg atataattgc agaaattcgt gcggaatctt
    13621 atatgacaga gattaatata ctgagtgttt tacctgttag tgaagaagat gactatatag
    13681 agcgtgtcaa ggtaagaaat aatcaagcaa taaaagcttt aaacaaaacc ttatcagtaa
    13741 tttctggcat taattatatt gaattgtatg atttattagt tgacgaaaaa ggtcagcttg
    13801 ctagcagttt tacaaaagat ggtttgcacc taacagatca agcctatgct aaaatttctg
    13861 aaacgataaa actccacctt taaaagtgtt tcgatactat ttttaagtat ttaaatacac
    13921 agaattagtg aaaagacctt ctaagtagaa agttcatgtc acgggtagta ccagtatttg
    13981 gcgaatcaat gtcagtaact ggtctttttt aatattatga taagaaaaaa caagaggggt
    14041 aaatgatggt aaaacaagta tttccaaaag gttttttatg gggcggggca actgctgcca
    14101 atcaatgtga aggagcttac aatgttgatg gacgtggcct agcaaatgta gatgttgttc
    14161 ctactggaga agatcgattt gcaattattt caggacaaaa gaaaatgttt gattttgagg
    14221 aaggatactt ttatccagca aaagaatcaa tagattttta tcaccattat aaagaagacc
    14281 ttgcattact tgcagaaatg ggtttcaaaa cctatcgtat gtcaatagca tggacgcgta
    14341 tttttccaaa gggtgatgag ctatatccaa ataaagctgg tcttcggttt tatgaaaata
    14401 tttttaaaga gtgtcgtaag tatggtattg aacctttggt aaccatcaca cattttgact
    14461 gtcctatcta ccttattaaa cattacggtg ggtggcggag ccgtaaaatg attggttttt
    14521 atgagcgcct tgtacgagct ttatttactc gttttaaggg gttagttaaa tattggttga
    14581 cctttaacga aatcaatatg attttacacg ctccttttat gggcgctggc ttatattttg
    14641 aagatggtga aaatcaagag caaattaaat atcaagcagc tcaccatgag ttagtggctt
    14701 cggctattgc agtaaaaatt gctcatgaag ttgatccaaa taatcaaatc ggatgtatgc
    14761 tagctgcagg tcaatactat ccaaatacgt gtcatccaca agattattgg gcttcgatgc
    14821 aaaaaaatag agaaaattat ttttttattg atgtgcaagc tcgtggcaaa taccctaatt
    14881 atgctaaaaa atattttgag catttaggta tttcaattca aatgacagca gaagatcttg
    14941 ctttgttaag agactatacg gttgatttta tttcattttc ttactattca agccgagtag
    15001 cttcaggtaa tccaactgtc agtgaacagg ttcaagaaaa tattttcgca tcacttaaga
    15061 atccttactt gaaatcttct gaatggggat ggcaaattga tccgcttgga ttacgtatta
    15121 ccttaaatgc tatctgggat cgttatcaaa aaccgatgtt tattgttgaa aatggacttg
    15181 gggcagtaga tattcctgat gagaatggtt acgtagaaga tgattatcgt attgattacc
    15241 tccgtcagca cattgctgca atgagagatg ctatatatgt agatggtgtt aatttaattg
    15301 gctatactac atggggatgt attgatttag tatctgcagg aactggtgaa atggaaaaac
    15361 gctatggttt catttatgtt gatcgcaata ataaaggaga gggaacactc aagcgttaca
    15421 agaaaaaatc gttttattgg tacaagaagg ttattgcaag taatggcagt caaatagaat
    15481 aaagagcaga ctaataactg ttaactctct ttttctcatt cataaaatag tgtataataa
    15541 ttgaaagaaa ttaagattaa taaggagatt tcatgaaatt tgaaaaacgg caggtctatt
    15601 atgttgtcat aacatttgct atttgctatg ctatacaggc ttattgggga gctgtttcta
    15661 atattttaac tacgcttcat aaggcaatat ttcctttttt gatgggagct ggaattgcct
    15721 atattattaa tattgtaatg tcagtctatg agcgattata tataaagctt tttaaaggat
    15781 ctagactatt aatggcaatc aagcgtagtg tttctatgat tttatcctat gcaactttta
    15841 ttggtttaat tgtctggcta ttttcaattg tcattccaga tttgatttct agtttgagtt
    15901 ctttattggt tattgatacc ggagcacttg ctaaattggt taataatctc aatgaaaata
    15961 aacaaatttc tgaggcttta aattatatgg gaacagataa agacttagtt tctactttaa
    16021 gtggttatag ccagcagatt ttgaagcaag ttttatctgt tttaacaaat ttactaacct
    16081 cagtttcctc tattgcggca acactcctga atgtttttgt tagttttatt ttttcaattt
    16141 acgttttggc aaacaaggag cagttgggac gtcagtttaa tttgttaatt gatacctatt
    16201 taggttcaac aggcaaaaca ttccattacg ttcgtcatat ccttcatcaa cgtttccatg
    16261 gtttttttgt aagccaaact ttagaagcta tgattttagg aagtttgacg gttattggta
    16321 tgttgatctt ccaatttcct tatgctttaa cagttggggt tttagttgct tttacagctc
    16381 taataccggt tgtgggagcc tacattggtg ttacaatcgg tttcatctta attgctactg
    16441 aatcgcttac tgaagcattc ttgtttgttc ttttcttgat ccttttacaa caatttgagg
    16501 gaaatgtcat ttatccgaaa gttgtcggtg gatcgattgg actgccttct atgtgggttt
    16561 taatggctat tactatcgga ggtgctttat gggggatctt aggcatgtta cttgctgttc
    16621 ctgttgcagc tactatctat cagattgtaa aagatcatat tatcaagcga caaacgctta
    16681 gaaatcgtgc acgaacctat cgttaaataa aaaatactta gatttttcat ctaagtattt
    16741 tttattgatt taaaacatga gtttttgatt ggtttacgaa tattatgact ggaggtgttt
    16801 tatgtaccat attgaattaa aaaaggaagc tttactacca agagaacgcc tagttgattt
    16861 aggcgcagat agattgagta atcaggagtt attagccatt ctcttacgta caggtattaa
    16921 agaaaaacct gttctggaaa tttcaacgca aattttagaa aacataagca gtttagcaga
    16981 ttttggtcaa ttatccttac aggagttgca atccattaaa ggaatcggtc aggttaaatc
    17041 cgtcgaaata aaagctatgc tagaactagc aaaacggatt cacaaagctg aatatgatcg
    17101 taaagagcaa attttaagta gtgaacaatt agcgaggaaa atgatgctcg aattagggga
    17161 taaaaaacaa gaacatttag tagctattta tatggataca caaaatcgta ttatcgaaca
    17221 gagaacaatt tttattggta ctgtacgtcg ttcagtagca gagccaagag aaattctaca
    17281 ttatgcttgt aaaaacatgg caacttcttt gattatcata cataatcatc cctcaggttc
    17341 tccaaagccc agtgaaagtg atttaagttt cactaaaaaa ataaaacgat catgtgatca
    17401 tctgggaatt gtttgcctag atcacatcat cgttggaaaa aataaatatt atagttttcg
    17461 agaagaagca gatattttat aaataatgaa ctatttattg ctgctggttc atgaaataaa
    17521 gtaaagtttg taattcgctt gttaaatcta catactgaac aatgatatct tttggtaatg
    17581 ttaaatgaac tggagaaaaa ctcaagatgc cttttatacc tgctttgact aagatgtcag
    17641 caacttcttg ggcttctgta ctaggtactg ttaggatagc agtttcaata tcactatcta
    17701 ttaaatggtc attgatagtc gaaataccgt agacaggaat gccatcctcg gttgttttac
    17761 caactaaatc attgctatct aaatcaaaag ccattgaaat ttgcatttta ttgcgatcgt
    17821 ggaaacgata atgcaagaga gctctaccga tatttccaca tcccaccaac ataacatttg
    17881 ttgtagaatg atcgttcaat atttctgcaa agaagttcat aagttttttg acatcataac
    17941 caaaaccacg gcgtcctagt tcaccaaaat aagaaaaatc acgtcgaaca gtagcagaat
    18001 cgatacctag ggcatctgca atttgtttgg aactagcttt ttcgatgcca tcagtattaa
    18061 aacgtttaaa aatacggtag tacagtgata aacgtttagc agttgcttta ggaatagact
    18121 tatccataat cacaaaatta tctccttgtt aaaaatttta ttattcgatt tagaattagt
    18181 tatataactt aattcttcac atctctattg tacaaaaaga ttgtgaaagt gacaacaaaa
    18241 tggttcgcaa aaagaccact aaaatgttaa aaatagtggt cgataaatta ttttgcagtt
    18301 ttcgtaaaga tatcacgctc aacgaggctg tccattaaga aataaaattt gtcttgaaga
    18361 attttatgat cggtagattt aaagatattt actattctaa ggccagattt atcagtaatt
    18421 aagagcttaa aaccagaaag ttctttattg attgtaatct ttagaggaaa gccatcgcca
    18481 ga
//
LOCUS       DATRTN010000030        18274 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.030, whole genome shotgun sequence.
ACCESSION   DATRTN010000030 DATRTN010000000
VERSION     DATRTN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18274
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.030"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            1..1231
                     /locus_tag="VBE22_001048"
                     /pseudo
     CDS             1..1231
                     /locus_tag="VBE22_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710501.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            1277..2116
                     /locus_tag="VBE22_001049"
     CDS             1277..2116
                     /locus_tag="VBE22_001049"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEO7202517.1"
                     /translation="MLIIELLKALFLGVVEGVTEWLPVSSTGHLILVQEFMKLNQSKS
                     FVEMFNIVIQLGAIMAVIVIYFKRLNPFQPGKSAREIRLTWQLWLKVVIACIPSILIA
                     LPFDNWFEAHFNFMIPIAIALIFYGFVFIWVEKRNAHLKPQVTELASMSYKTAFLIGC
                     FQVLSIVPGTSRSGATILGAIIIGTSRSVAADFTFFLAIPTMFGYSGLKAVKYFLDGN
                     VLSLDQSLILLVASLTAFVVSLYVIRFLTDYVKRHDFTIFGKYRIVLGSLLILYWLVV
                     HLF"
     gene            2236..2991
                     /gene="mecA"
                     /locus_tag="VBE22_001050"
     CDS             2236..2991
                     /gene="mecA"
                     /locus_tag="VBE22_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEO7202518.1"
                     /translation="MEMKQISETTLKITISMEDLEDRGMELKDFLIPQEKTEEFFYSV
                     MDELDLPENFKNSGMLSFRVTPKKDRIDVFVTKSELSKDLNLEELADLGDISKMSPED
                     FFKTLEQSMLEKGDTDAHAKLAEIENMMDKATQEVVEENVSEEQPEKEVETIGYVHYV
                     FDFDNIEAVVRFSQTIDFPIEASELYKNGKGYHMTILLDLENQPSYFANLMYARMLEH
                     ANVGTKTRAYLKEHSIQLIHDDAISKLQMIEMG"
     gene            2993..4153
                     /locus_tag="VBE22_001051"
     CDS             2993..4153
                     /locus_tag="VBE22_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEO7202519.1"
                     /translation="MIPFTIEYIFVLIGAFLLSIILTPIIRVISLKVGAVDKPNARRI
                     NKVPMPSSGGLAIFLSFVVTTLFFMPMAASRHFIEVSYFHYILPVIIGGLVVTTTGFI
                     DDIFELRPRYKMLGIIIAAVIIWKFTHFRFDSFKIPIGGPLLEFGPILTFFLTVLWII
                     SITNAINLIDGLDGLVSGVSIISLATMAVVSYFFLPKIDFFLTLTIVILIASIVGFFP
                     YNYHPAIIYLGDAGALFIGFMIGVLSLQGLKNSTAVAVITPVIILGVPILDTAVAIVR
                     RKLSGKKISEADKMHLHHRLLSMGFTHRGAVLVVYGIAIIFSLIALLLNVSSRIGGIF
                     LLLALLLAMEIFIEGLNIWGENRTPLFNLLKFIGNSDYRQSVIAKYSDKHQK"
     gene            4318..5088
                     /gene="sufC"
                     /locus_tag="VBE22_001052"
     CDS             4318..5088
                     /gene="sufC"
                     /locus_tag="VBE22_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEO7202520.1"
                     /translation="MSVLEIKNLHVSIEDKEILKGLNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTAGEILFDGEDILELEVDERARLGLFLAMQYPSEVPGITNAEFIR
                     AAMNAGKADDDKISIRQFITKLDEKMELLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGEGFGAMIITHYQRLLNYITPDKVHV
                     MMDGKVVLSGGPELAVRLEKEGYAQIAEELGLEYKEEV"
     gene            5125..6387
                     /gene="sufD"
                     /locus_tag="VBE22_001053"
     CDS             5125..6387
                     /gene="sufD"
                     /locus_tag="VBE22_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEO7202521.1"
                     /translation="MSKEAILNFLQAKGEPTWLQELRLKAFEKIEELELPVIERVKFH
                     RWNLGDGTILENDYTANIPDFTELGNNPKLVQIGTQTVLEQVPMELIEKGVVFTDFYS
                     ALEEIPEVIERYFGKARPFEEDRLAAYHTAYFNSGAVLYIPDNVEITQPIEGLFYQDN
                     QSKVPFNKHILLIVGKNAKVSYLERFESIGDGTERTSANISVEVIAQAGSQIKFASID
                     RLGENVTTFISRRGRHSSDATIDWALGVMNEGNVVADFDSDLIGDGSHANLKVVAASS
                     GRQVQGIDTRVTNYGCNSVGHILQHGVILERGTLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENDVTAGHAASIGQVDPEDLYYLMSRGLNQKTAEQLVIRGF
                     LGTVIAEIPVKEVRDEMIAVIDTKLEKR"
     gene            6389..7621
                     /locus_tag="VBE22_001054"
     CDS             6389..7621
                     /locus_tag="VBE22_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO7202522.1"
                     /translation="MTLLDSYKLKQDFPILNQLVNDEPLIYLDNAATTQKPNQVLETL
                     RAYYQNDNANVHRGVHTLAERATAQYETAREKARQFLNAKSSKEILFTRGTTTGLNWV
                     AKFAESILERGDEVLISIMEHHSNIIPWQQACERTGAKLVYAYLKDGSLDLEDFYNKL
                     SSKTKFVSLAHISNVLGCITPVKAIAERVHQVGAYMVVDGAQSAPHMAIDVQDLDCDF
                     FALSGHKMLGPTGIGVLYGKESILDKMPPVEFGGEMIDFVYEQSATWKELPWKFEAGT
                     PNIAGAIAFGEALDYLTDVGMDEIHQYEQSLVSYVLPKLQAIDGLTIYGPSDAESHVG
                     VIAFNLEGLHPHDVATAMDYEGVAVRAGHHCAQPLINHLGIHSAVRASFYFYNTKEDC
                     DKLVDAIQKTKEFFNGTL"
     gene            7608..8051
                     /locus_tag="VBE22_001055"
     CDS             7608..8051
                     /locus_tag="VBE22_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEO7202523.1"
                     /translation="MALSKLDNLYMAVVADHSKHPHHHGFLEGVEQVQLNNPTCGDVI
                     SLSVKFDGNIISDIAFAGNGCTISTASSSMMTDAVIGKTKEEALQLADVFSKMVQGDQ
                     NPKQEKLGDAEFLAGVSKFPQRIKCATLSWNALRKAIERDNQAEL"
     gene            8151..9569
                     /gene="sufB"
                     /locus_tag="VBE22_001056"
     CDS             8151..9569
                     /gene="sufB"
                     /locus_tag="VBE22_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEO7202524.1"
                     /translation="MSEINEKVEPQPIDLGEYKFGFHDDVKPIYSTGKGLNEAVIREL
                     SAAKGEPEWMLDFRLKSLETFNKMPMQTWGADLSDIDFDDIIYYQKASDKPARDWDDV
                     PEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKLGIVFTDTDSALKEY
                     PELFKKYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRATAKKDATVEWIDGNLGAKTTMKYPSVYLDGEGACGTMLSIAFANKGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKDSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSEAEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(9641..10828)
                     /locus_tag="VBE22_001057"
     CDS             complement(9641..10828)
                     /locus_tag="VBE22_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO7202525.1"
                     /translation="MPKLIVSFLCILLSLTCVNSVQAEEHKDIMQITREAGYDVKDIN
                     KPKASIVIDNKGHILWEDNADLERDPASMSKMFTLYLLFEDLAKGKTSLNTTVTATET
                     DQAISKIYEISNNNIHAGVAYPIRELITMTAVPSSNVATIMIANHLSQNNPDAFIKRI
                     NETAKKLDMTKTHFYNPSGAVASAFNGLYSPKEYDNNATNVTTARDLSILTYHFLKKY
                     PDILNYTKYPEVKAMVGTPYEETFTTYNYSTPGAKFGLEGVDGLKTGSSPSAAFNALV
                     TAKRQNTRLITVILGVGDWSDQDGEYYRHPFVNALVEKGFKDAKNISSKTPVLKAVKP
                     KKEVTKTKTKSIQEQPQTKEQWWTKTDQFIQSHFVSILIVLGTIAILCLLAGIVLLIK
                     RSR"
     gene            complement(10981..12216)
                     /locus_tag="VBE22_001058"
     CDS             complement(10981..12216)
                     /locus_tag="VBE22_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO7202526.1"
                     /translation="MKKIITSILLLSCIFFMPTISAESFNASAKYALAVDLDSGKILY
                     EKDANRPAAIASLTKIMTVYMVYKEIDNGNLKWNTKVNISDYPYQLTRESDASNVPLE
                     KRRYTVKQLVDAAMISSANSAAIALAEHISGTESKFVDKMTAQLEKWGIHDSHLVNAS
                     GLNNSMLGNHIYPKSSQNDENKMSARDIAIVAYHLVNEYPSILKITSKSVAKFDKDIM
                     HSYNYMLPDMPVFRPGITGLKTGTTELAGQSFIATSTESGMRLLTVIMHADKADKDKY
                     ARFTATNSLLNYITNTYEPNLVLAKGATYKGKEASVRDGKEQSVIAVAKNDLKVAQKK
                     NITKQNQLKINFKKELTAPITKKENLGKAYYVDLNKVGKGYLIKEPSVHLVAKDSIER
                     SFFLKVWWNHFVRYVNEKL"
     gene            12458..14113
                     /locus_tag="VBE22_001059"
     CDS             12458..14113
                     /locus_tag="VBE22_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7202527.1"
                     /translation="MAKQKNNWRRVGVGVLTLASVATLAACGSKSASQDSNGAINWAI
                     PTEINTLDLSKVTDTYSNLAIGNSSSNFLRLDKDGKTRPDLATKVDVSKDGLTYTATL
                     RKGLKWSDGSKLTAKDFVYSWQRLVDPKTASQYAYLAVEGHVLNADKINEGQEKDLNK
                     LGVKAEGDDKVVITLSSPSPQFIYYLAFTNFMPQKQEVVEKYGKDYATTSKNTVYSGP
                     YTVEGWNGSNGTFTLKKNKNYWDAKNVKTKEVRIQTVKKPDTAVQMYKRGELDAANIS
                     NTSAIYQANKNNKDVTDVLEATTAYMQYNTTGSVKGLDNVKIRRALNLATNRKGVVQA
                     AVDTGSKPAIAFAPTGLAKTPDGTDLAKYVAPGYEYNKTEAAKLFKEGLAESGLTKLK
                     LTITADADVPAAKNSVDYIKSTWEAALPGLTVEEKFVTFKQRLEDSRKQNFDIVVSVW
                     GGDYPEGSTFYGLFKSDSQNNDGKFANKDYDAAYNKAISEDALKPEESAKDYKEAEKI
                     LFEQGAYNPLYFRSGKGLQNPKLKGVIRNTTGLSIDFTHAYKK"
     gene            14232..15146
                     /locus_tag="VBE22_001060"
     CDS             14232..15146
                     /locus_tag="VBE22_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7202528.1"
                     /translation="MIKYILKRVAILLVTLWVVITLSFFLMQILPGTPYNNPKLTEEM
                     IALLNKQYGLDKPVWQQYLTYLWNVLHGDFGTSYQSVNQPVSRMISLRLGVSVHLGVQ
                     ALVFGVLGGILVGAISARHKNDKVDGILSVIATLGISMPSFIIGILLLDYFGFKWNLL
                     PLSGWGTFSQTILPSLALGLPTLASVSRFFRSEMIETLNSDYVQLARSKGMTIRQVTR
                     KHAYRNSMIPILTLIGPLAAGLLTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTT
                     IVYAVMLMVAILITDVVISIVDPRVRLQ"
     gene            15156..16187
                     /locus_tag="VBE22_001061"
     CDS             15156..16187
                     /locus_tag="VBE22_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7202529.1"
                     /translation="MADKNRTFKLVGAGSSSTQEKIEKPALSFMQDAWRRLKKNKLAV
                     VSLYLLALLLTFSLASNLFVTQKDANGFDSKKVTTYRNLPPKLSSNLPFWNGSIKYAG
                     NTESTDAYKSQNVPEKVKYALGTDSLGRSVAKRIIVGIRISLLVAIAATFIDLIIGVT
                     YGLVSGFAGGRLDTLMQRIVEVISSIPNLVIVTMLGLVLGNGITAIIISIAFTGWTSM
                     SRQVRNLTLSYREREFVLAARSLGESPIKIAFKHILPNISGIIIVQIMMTIPSAIMYE
                     AVLSAINLGVKPPTASLGSLISDAQENLQYYPYQVILPALALVMISLAFILLGDGLRD
                     AFDPKSSDE"
     gene            16200..17246
                     /locus_tag="VBE22_001062"
     CDS             16200..17246
                     /locus_tag="VBE22_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202530.1"
                     /translation="MEKETILSVNNLHVDFHTYAGEVKAIRDVNFELKKGETLAIVGE
                     SGSGKSVTTRTLIGLNAKNSEISGNVQFKGRNLVELSEEEWTKVRGNEISMIFQDPMT
                     SLDPTMKIGMQIAEPMMIHQKISKKDALKLALELMKDVGIPNAEEHINDYPHQWSGGM
                     RQRAVIAIALAADPEILIADEPTTALDVTIQAQILNLMKKIQAERDSSIVFITHDLGV
                     VAGMADRVAVMYAGKIVEFGTVDEVFYNPQHPYTWGLLNSMPTTDTESGSLESIPGTP
                     PDLLNPPKGDAFAARNEFALDIDHEEEPPYFKVSETHFAATWLLDERSPKVLPPLPIQ
                     KRWEKWNEIEGRKA"
     gene            17246..18178
                     /locus_tag="VBE22_001063"
     CDS             17246..18178
                     /locus_tag="VBE22_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7202531.1"
                     /translation="MTENRKKLVEVKNVSLTFNKGKANEVRAIDNVSFDIYEGEVFGL
                     VGESGSGKTTVGRSILKLYDISDGEITFNGEVISHLKGKALHSFRKDAQMIFQDPQAS
                     LNGRMKIRDIVAEGLDIHKLAKSKSDRDSKVQALLDLVGLNKDHLTRYPHEFSGGQRQ
                     RIGIARALAVEPKFIIADEPISALDVSIQAQVVNLMQKLQREQGLTYLFIAHDLSMVK
                     YISDRIGVMHWGKLLEVGTSDDVYNNPIHPYTKSLLSAIPEPDPESERQRVHQPYNPA
                     IEQDGQERQMHEITPCHFVLSTPQEAEEYKKQIL"
BASE COUNT         5840 a         2951 c         3607 g         5876 t
ORIGIN      
        1 aaacagtaaa aataattata agaaaaaaat tgaacattaa gagaagatga tagagcgtca
       61 tggttttatc ctaagtttct tgttaaggat attattacct tcattcttta ttattgtgac
      121 actattcatc tcaattaggg tgttcctgtt tagaaaaaaa gttaataaat acgggcaatt
      181 ccctaaggat catcatttat atgaagcacc tgaggacctt tcaccactag agttaactca
      241 aagcatttat agtatgagtt ttaaaaattt tcaagatgag gagaagaaaa ctcaccttat
      301 cagtcaagaa caactcattc agtcaattct attagacttg attgatagaa aagtattgaa
      361 ttatgatgat aacttgttat ctctagctaa cttagataga gcttctgatg cagaaataga
      421 ttttatagag tttgcttttg cggattctac gagtttgaag ccagaccaac tcttttctaa
      481 ttaccaattt agttataaag aaacactacg tgaactgaaa aagcagcaca aggcttcaga
      541 tctgcaaact caaatgagac gccgaggaag taatgcctta tcaagaatta cgcgtctcac
      601 gaggttgatt tctaaagata atataaactc tcttagaagc aagggaattt catcacctta
      661 tcgtaaaatg tcttcagaag agtctaaaga attatctagg ttgaaaagat tcagttacct
      721 atcacctctt atttcttttg ttgttataat ttatacgctt tttttaaatt attttaccta
      781 tttctgtatc tatctcttat tgtttgttgt tatcctgttg ttgaataaaa tcatttttat
      841 gatgacaaga aaaataagta acggttatat tgtaactgaa gatggagcaa gtcgtatcta
      901 ccaatggact agttttagga acatgctaag ggatatcaaa tcgtttgatc gttcagagtt
      961 agaaagtatc gtactatgga atcgaatatt ggtttacgct actttattcg gctacgctga
     1021 ccgtgttgag aaagtactca gagtgaacca aatagatatt ccagaaagat ttgcaaacat
     1081 tgatagtcat cgatttgcga tttcagtcaa tcaatctagt aatcattttt caacgataac
     1141 tgaagatgtt agtcacgctt ctaattttag tgttaattca ggcggttctt caggtggttt
     1201 ctcaggcggc ggaggtggcg gtgccttcta attttccaaa agtatggtat aataaagaaa
     1261 aatttatgga gttattatgt taataattga attactcaaa gccctatttt taggagtcgt
     1321 tgagggagtg acagaatggt tacctgtttc tagtactggc catttaatct tggttcaaga
     1381 gtttatgaag ttgaaccaaa gcaaatcctt tgttgaaatg tttaatattg ttatacaact
     1441 tggggcaatt atggcagtta ttgtcattta ttttaaacgc cttaacccat ttcagccagg
     1501 taaatctgct agagaaattc gtttaacttg gcaattatgg ttaaaagtag tgatagcttg
     1561 tattccatct attttaattg ctttaccatt tgataattgg tttgaagctc attttaattt
     1621 catgatcccg attgcaatag ccctaatctt ttatggtttt gtcttcatat gggttgaaaa
     1681 acgtaatgca cacctcaaac cacaggtaac cgaattggca agtatgtctt ataagacagc
     1741 tttcttgatt ggatgcttcc aggttctcag tattgttccg ggaaccagtc gttctggagc
     1801 tactatttta ggagcaatta ttattggaac tagtcgttcg gtcgctgctg actttacttt
     1861 cttccttgcc atcccaacta tgtttggtta tagtggactt aaggcggtta aatacttttt
     1921 agatggtaac gtcttgagtt tagaccaatc tttaatactt ttagtagcaa gtctgacagc
     1981 tttcgtagtt agtttatatg ttattcgttt cttgacagac tatgtcaaac gacacgattt
     2041 caccatcttt ggtaagtatc gtatagtctt aggaagttta ctcatcctct actggttagt
     2101 tgttcattta ttctaaaata taacataaaa agctgagtat tcctcagctt tttagcgtgt
     2161 taggctgcga aaaattgcta aaaaatagct ttttcggtat aataagaaaa ctgaatactc
     2221 gtatgaggtg atgatatgga aatgaaacaa atcagtgaaa caacactgaa aattacaatt
     2281 agtatggaag atttagaaga tcgtggtatg gagctgaaag atttcctaat ccctcaggag
     2341 aagactgagg aatttttcta ttctgtcatg gatgaattag acttgccaga aaactttaaa
     2401 aatagtggta tgttaagttt tcgagtaaca cctaaaaaag atcgcattga tgtttttgtt
     2461 acaaagtctg aattaagtaa agatttaaat ttagaagaat tagcagattt gggtgacatt
     2521 tcaaaaatgt ctccagaaga cttttttaaa acattggaac aatcgatgtt ggaaaaaggg
     2581 gatacggatg cccatgccaa attagcagaa attgaaaata tgatggataa agcaactcaa
     2641 gaagtagttg aggaaaatgt ttctgaagaa caacctgaaa aggaagtaga aacgattgga
     2701 tatgttcact atgtctttga ttttgataat attgaagctg tagttcgatt ttcacaaacg
     2761 attgattttc caatagaagc ttctgaactc tataaaaatg gtaaaggcta tcatatgaca
     2821 attttgctag acttggaaaa ccaaccaagc tactttgcta atttgatgta cgctcgaatg
     2881 cttgaacatg ctaatgtggg cacaaaaaca cgagcttact tgaaagagca tagtattcaa
     2941 ttgattcatg atgatgctat ttcaaaatta caaatgattg agatgggata aaatgatacc
     3001 ttttacaatt gagtatattt ttgtattgat tggagcattt ttattatcaa tcattctaac
     3061 tcctattatt cgagttatat ctttgaaggt aggagcggtt gataaaccga acgctcgaag
     3121 aatcaacaaa gtaccaatgc caagtagtgg tggtttagca atatttctct cctttgttgt
     3181 aacaacatta tttttcatgc caatggcggc cagtagacac tttatagaag tctcttattt
     3241 tcactacatt ttgcctgtaa ttattggcgg tctcgtagtt acaacaacag gatttataga
     3301 tgacatcttt gaattaagac ctcgttataa aatgttggga attattattg cagctgttat
     3361 catatggaaa tttacacatt ttagatttga tagttttaag attccaatag gtggtccact
     3421 cttggagttt ggtcctattt taactttctt tttgacagtt ttatggatta tttcaatcac
     3481 taatgctatc aatctgattg atggtctaga tggtctagtt agtggtgttt caatcatcag
     3541 tctagcaacg atggcagtgg tttcttattt ctttttacct aaaatagatt tttttctcac
     3601 gctaactatt gttattttaa tcgcctcaat agttggtttt ttcccttata attatcatcc
     3661 agccattatc tacctaggtg atgctggtgc cttgtttata ggatttatga ttggtgtatt
     3721 atcgcttcaa ggtttaaaaa actccacagc tgttgcagtt ataacacctg tcattatttt
     3781 aggagttcct attttggata cagctgttgc tattgtgagg cgtaaattat caggaaaaaa
     3841 gatttctgaa gctgataaga tgcacctgca tcaccgattg ctatctatgg gatttacaca
     3901 tagaggagca gttttagttg tttatggtat tgcaattatc ttttcgctta tcgctttgct
     3961 tttaaatgtt tctagtcgta ttgggggaat ttttcttcta ttggcgctac tattagcaat
     4021 ggaaattttt atagaaggtt taaatatttg gggtgagaat cggacaccac tatttaatct
     4081 cttaaagttc attggtaaca gtgattacag acaaagtgtt attgctaaat atagcgataa
     4141 gcatcaaaaa taaaagcctg aaaaggcttt tttagtttta atcatttaga tagcagtcac
     4201 acctcatttt tgctataatt atttagaata taatatcggg tgagtgccct ataatatctt
     4261 ctttaaacca tagagaaata tcacatattc tctgttaacc aagaaaagga gtgtacaatg
     4321 tctgtacttg aaatcaaaaa tcttcatgtt tctattgaag ataaagaaat tttaaaaggt
     4381 cttaatttaa cgttgaaaac aggagaaatt gctgctatca tgggtccaaa tggtactggg
     4441 aagtcgacac tttcagcagc tattatggga aatcctaatt atgaagttac agcaggtgaa
     4501 attctttttg atggtgaaga tattttagag ctagaagtgg atgaacgtgc tcgtttgggt
     4561 ctatttcttg ccatgcaata cccttcagag gttccaggga ttactaatgc tgaatttatc
     4621 cgagcagcta tgaatgcggg aaaggctgat gatgataaaa tttccatccg ccaatttatc
     4681 actaagttag atgaaaagat ggaactactt ggcatgaaag aagaaatggc ggaacgttat
     4741 cttaacgaag gtttttcagg tggtgagaag aagcgcaatg aaattttaca actcctaatg
     4801 ttagagccta agtttgccct cttggatgag attgactctg gccttgatat tgatgctctt
     4861 aaagtggtat caaagggagt caatgaaatg cgtggtgaag gttttggtgc tatgattatt
     4921 acacactatc aacgcctttt gaattacatt acaccagata aagtacacgt tatgatggac
     4981 gggaaggtag tattgtcagg aggaccagaa ttagcggttc gccttgagaa agaaggttat
     5041 gctcaaatcg ctgaagagct tggtttagag tataaagaag aagtctaatt tgtcacttca
     5101 actagaagag aaaaggagtt acaaatgtct aaagaagcta tattaaattt tttacaagcc
     5161 aaaggtgagc ctacttggtt acaagaatta cgactaaagg cttttgaaaa aatagaagaa
     5221 ctagaattac cagtaattga acgtgtaaaa tttcatagat ggaatttagg tgatggaact
     5281 attttagaga atgactatac agctaacatt ccagatttca cagagttagg aaacaatccc
     5341 aagttggttc aaattgggac ccaaactgtc cttgaacaag ttcctatgga attaatagag
     5401 aaaggcgttg tctttacaga tttctattcg gctttggaag aaattccaga agtcatagag
     5461 cgctactttg ggaaagcacg tccttttgaa gaagatagac ttgctgccta tcacacagct
     5521 tacttcaaca gtggtgcagt gctttatatt ccagataatg ttgaaattac tcaaccaatc
     5581 gaaggtcttt tttatcaaga taatcaatct aaagtaccat ttaataagca tatccttctt
     5641 attgttggaa agaatgcgaa agttagttac ttagagcgat ttgagtcaat tggtgatggg
     5701 acagaacgaa caagtgctaa tatttctgta gaagtaattg cccaagcagg tagccaaatt
     5761 aaatttgctt caattgatcg cctcggagaa aatgtgacaa cgtttataag tcgtcgtggt
     5821 cgtcacagta gcgatgcaac aatagattgg gcccttgggg tgatgaatga gggcaatgtt
     5881 gttgctgact ttgattctga tttaattggt gatggaagcc acgctaattt aaaagtagtt
     5941 gctgcctcgt cagggcgtca agttcaagga attgatacgc gtgttacaaa ctacggctgt
     6001 aattcagtag gacatatttt acagcatggg gttattttag agcgtgggac tcttactttt
     6061 aacggtatcg gacatattat aaaaggtgca aagggagcag acgcccaaca agaaagccga
     6121 gttctcatgt tatcagacaa agcacgttca gatgctaatc ctatcctttt gattgatgaa
     6181 aatgatgtta cagctggtca cgcagcctca attggtcagg ttgacccaga ggatttatat
     6241 tatttgatga gccgtggctt gaaccaaaaa acagcagagc aattagttat tcgtggattt
     6301 ttaggaacag tcattgctga aataccagta aaagaagttc gcgatgaaat gattgccgtt
     6361 attgatacaa aactagaaaa acgttaatat gacattacta gatagttata aactaaagca
     6421 agatttccca attttaaatc aattagttaa tgatgagccg cttatctatt tagataatgc
     6481 agcaacgaca caaaaaccga atcaagtttt agaaactttg cgagcttact accaaaacga
     6541 caatgcaaat gtccatcgtg gtgttcatac tttagcagaa cgagcgactg ctcagtacga
     6601 gactgctcgt gaaaaagcaa ggcagttttt aaatgctaaa tcaagtaaag aaattttatt
     6661 cacacgtggc accacaacag gattaaattg ggtagctaag tttgccgaaa gtattttaga
     6721 acgaggtgat gaggtactta tttccattat ggagcatcat tctaatatta tcccatggca
     6781 acaagcttgt gagagaactg gtgctaaatt ggtttatgcc tatctaaaag atggttcttt
     6841 agatttagaa gatttctata ataaattatc ctctaaaact aaatttgtta gtttggcaca
     6901 tatctcgaat gtacttggct gtattacacc agtaaaagca atagcagaac gtgtgcatca
     6961 agttggggca tacatggttg ttgatggagc tcaatcagcg ccacatatgg cgattgatgt
     7021 tcaagacctt gattgtgatt tcttcgcctt atctggccat aagatgcttg gtcctactgg
     7081 tattggtgtt ctttatggga aagaaagcat tttggataag atgccacctg tcgaatttgg
     7141 tggtgaaatg attgattttg tctatgagca aagtgcaaca tggaaggaat tgccctggaa
     7201 gtttgaagct ggaacgccta atattgctgg agcgatcgct tttggagaag ctttagatta
     7261 tttaacggat gttggtatgg atgagattca tcaatatgaa caaagtttag tttcttatgt
     7321 gttgcctaaa ttacaggcta tcgatggttt aactatttac ggacctagtg atgctgaatc
     7381 tcatgtaggc gttattgcct tcaatttaga aggtttacac ccacatgatg tggcaactgc
     7441 aatggattac gaaggagtgg ctgttcgtgc aggtcatcat tgtgcacaac cccttattaa
     7501 tcatcttggt atccattcgg cggttcgagc aagtttctat ttctataata ccaaagagga
     7561 ttgcgacaaa ttggtagacg ctatccaaaa aactaaggag tttttcaatg gcactctcta
     7621 aattagataa cctctacatg gcagtggtag ctgatcattc aaaacaccct catcatcatg
     7681 gatttttaga aggcgttgaa caagtgcagt taaataatcc tacatgtgga gatgttattt
     7741 ccttatctgt taaatttgat ggaaatatta tttcagatat tgcatttgct ggtaatggtt
     7801 gtacaatttc tacggcttca tcaagcatga tgacagatgc agttattggg aaaacaaaag
     7861 aagaagcatt gcagcttgct gatgtattct ccaaaatggt tcaaggcgat caaaatccaa
     7921 aacaagagaa acttggggat gcagagtttc tagctggggt atcaaaattt ccacaacgta
     7981 tcaaatgtgc tacgctttcg tggaatgctt taagaaaggc tattgagaga gataatcagg
     8041 cagaactctg acaaccatag ttgaaggaat tacatttaaa caacttttac tgacataagg
     8101 tagtctagta gaagaatgtc ttaaatttaa atattaaaga aaggataagt atgtccgaaa
     8161 taaatgaaaa agtagaacca cagccaatag atcttgggga atataaattt ggatttcatg
     8221 acgatgtaaa gccaatttat tctacgggaa aaggtctaaa tgaggctgtt attcgtgagt
     8281 tatctgcagc taagggtgaa cctgagtgga tgttggactt tcgtctaaaa tccttggaaa
     8341 cgtttaataa aatgccaatg cagacctggg gagcagattt atcagatatt gattttgatg
     8401 atattattta ttatcaaaaa gcatctgata aacctgcgcg tgattgggat gatgttccag
     8461 aaaaaatcaa agaaactttt gaaagaattg ggattccaga agctgaaaga gcttatcttg
     8521 caggagcatc agcacaatat gaatcagaag tggtttatca taatatgaaa gaagaatatg
     8581 ataagctggg tattgttttt acggatactg actctgcact taaagagtac ccagagctat
     8641 tcaaaaaata ttttgctaaa cttgtccctc caacagataa taaattagct gctctgaact
     8701 ctgctgtatg gtcaggtgga acatttattt atgtccctaa aggtgttaag gtggatattc
     8761 cacttcaaac ttacttccgt attaataatg aaaatactgg acaatttgaa cgtactctca
     8821 ttattgttga cgagggagca agtgttcact acgttgaagg ttgtaccgcc ccaacttatt
     8881 cttcaaatag tttacatgca gctatagttg aaatttttgc acttgatgga gcttatatgc
     8941 gctatacgac tattcaaaat tggtccgata atgtctataa tttagtgaca aaacgtgcta
     9001 ccgctaaaaa agatgcaaca gttgagtgga tagatggaaa tctaggtgct aaaacaacaa
     9061 tgaaataccc atcggtttac cttgatggtg aaggagcatg tggcacgatg ttgtctattg
     9121 cttttgcaaa caaaggacaa caccaagata cgggtgcaaa gatgattcat aatgctcccc
     9181 atactagttc atccattgtc tctaaatcaa ttgctaaggg tgggggaaaa gttgattatc
     9241 gaggtcaagt gacatttaat aaagattcca aaaaatcagt gtcacatata gaatgtgaca
     9301 ccatattgat ggatgatatt tcaaaatcag ataccatacc gtttaatgag attcataatt
     9361 cacaggttgc tttagagcat gaagcaaagg tgtctaagat ttctgaagag caactgtact
     9421 acttgatgag tcgaggttta tctgaagctg aagcaacaga aatgattgtt atggggtttg
     9481 ttgagccctt tacgaaagaa ttaccaatgg aatatgcggt agagttaaat cgtttaattt
     9541 cctatgaaat ggaaggttca gttggttaat tgaagatttc aagagataat aaaaagcccc
     9601 tatgttattg atgaaggggg cttttatatt agcacagtta ttatctagag cgctttataa
     9661 gtaatactat cccagctaaa agacaaagga tagcgatggt tcccagaaca attaaaatag
     9721 atacaaaatg tgattgaata aattgatctg tttttgtcca ccactgttct tttgtttgag
     9781 gctgttcttg aatagatttg gttttggttt tagtaacttc ttttttaggt ttaacggctt
     9841 ttaatacagg agttttagaa gaaatatttt tagcgtcttt aaaacctttt tctacaagag
     9901 cgttgacaaa cggatgacga tagtactctc cgtcttggtc tgaccaatcg ccaacgccta
     9961 aaatcacagt tatcaaacga gtattctggc gtttagctgt aactaaggca ttaaaagcag
    10021 cgctagggct agaaccagtt tttaagccat ctactccttc taatccaaat ttagcgccgg
    10081 gggtagagta gttataagtt gtaaatgttt cttcataagg agttccgacc atggccttga
    10141 cttcaggata ttttgtatag ttcagtatat cagggtattt tttaaggaaa tgataggtta
    10201 aaattgatag atcacgtgca gtcgtaacgt tagtagcatt gttatcgtat tcttttgggg
    10261 agtaaagtcc attaaaagca cttgctaccg ccccactagg gttataaaag tgagtttttg
    10321 tcatatcgag tttcttggcg gtttcattga ttcgtttaat gaaggcgtca ggattgtttt
    10381 gtgataagtg gttagcaatc ataatagttg ctacattaga tgacgggaca gccgtcatag
    10441 taatcagttc acgaatagga taagcaaccc cagcatgaat attgttatta ctaatttcat
    10501 aaatcttact tatggcttgg tctgtttctg ttgcagtcac tgtggtgttg aggcttgttt
    10561 ttcctttagc taagtcttca aatagtaaat ataaagtaaa cattttagac atgctagcgg
    10621 gatcacgttc taaatcagcg ttatcttccc acaaaatatg acctttattg tcaataacga
    10681 tagacgcttt aggtttatta atatctttaa catcatatcc ggcttctcgg gtaatttgca
    10741 taatatcttt atgttcttca gcttgcacag agtttacaca agtcagggat aataaaatgc
    10801 agaggaaaga tacgattaat ttaggcatag gtaactcctg aaaacttttt attaatatta
    10861 taacaaaaaa tcactaataa aaaagacatt tcattagtga tttttaattt tatatattct
    10921 cctaaaccta gctaaagaaa ctgctgtgca gtaactcctt gtgtcaaagg gagattacta
    10981 ttaaagtttt tcgttaacgt agcgcacaaa atgattccac cacactttga ggaagaaact
    11041 gcgctcaata ctatcttttg ccactaaatg aacgctaggt tcctttatga gatagccttt
    11101 tccaacctta ttaaggtcaa cgtaataagc tttccctagg ttctcttttt ttgtaatagg
    11161 agcagtaagc tcttttttaa agttaatttt taactgattt tgtttagtga tatttttctt
    11221 ctgtgctact ttcaaatcgt ttttagcaac agcgataacc gattgttctt ttccgtctct
    11281 cacacttgct tctttacctt tatatgtagc tcctttagct aatacaaggt taggttcgta
    11341 ggtgtttgtg atatagttta agagagagtt agttgctgta aagcgagcat atttgtcttt
    11401 atcggcctta tcagcatgca taataacagt gagtagtctc attccacttt cagtagatgt
    11461 agcaataaaa gattggccag ctaattccgt tgtcccagtt ttcaaacctg taatacctgg
    11521 tctaaagaca ggcatatctg gtagcatgta gttataagaa tgcataatat ctttatcaaa
    11581 tttagcaacg gacttactag taatcttaag aatggaagga tattcgttga ccaaatggta
    11641 ggcaacaata gcaatatcac gtgcactcat tttattttcg tcgttttgtg acgattttgg
    11701 ataaatgtga ttgcctaaca tactattatt taagccagaa gcattgacta ggtggctatc
    11761 atgaattccc cacttttcca attgagcagt cattttatca acaaatttac tttcagttcc
    11821 tgaaatatgt tcagccaaag caatggctgc actgttagca ctagaaatca tggcagcgtc
    11881 cacgagctgt ttaacagtat agcgcctttt ttctaaagga acattactag catcagattc
    11941 acgtgttagt tgataagggt agtcagatat atttactttg gtattccact tgaggttacc
    12001 gttatcaatt tctttataga ccatataaac ggtcattatt ttagtcaagg aagcaatagc
    12061 agcgggtctg ttagcatctt tttcatacaa gatttttcct gaatctaaat caactgccaa
    12121 ggcatattta gcggaagcat taaaagattc agcagagatg gttggcataa aaaaaatgca
    12181 actaagtaat agaatagaag taataatttt tttcaaaata acacctttag aaatttttta
    12241 aactttgtcc ttcctattat accatatcac tataccatac taggaattat tattttcaga
    12301 taattgaatg aagaaaacgt ttgttataaa aatgtaaaaa aatgtaaaaa aatgaaagtt
    12361 aaaaaattat ctgaaaattt tgcgtttttg tcttttctta agaatttttt gtggtataat
    12421 gttagtcact agataaatga attaggagaa caaagctatg gcaaaacaga aaaataactg
    12481 gcgccgtgtt ggagttggtg tccttacact tgcttcagtt gcgactcttg ctgcatgtgg
    12541 aagtaaatca gcttcccagg attctaatgg agcgattaat tgggctattc caacagaaat
    12601 caatacacta gatttatcta aagttacaga cacttactca aatctagcta ttggtaactc
    12661 tagtagtaat ttccttcgct tagataagga tggaaagacg agaccagatt tggctactaa
    12721 agttgatgtt tcaaaagatg gtttaactta tacagctaca ttacgtaaag gcttgaagtg
    12781 gtcagatggc agtaaactta ctgcaaagga ttttgtttat tcatggcaac gtttagttga
    12841 tcctaagaca gcttcacaat atgcttacct tgctgttgaa gggcatgtgc ttaatgccga
    12901 taaaatcaac gaaggacaag agaaagactt gaataagcta ggtgttaagg cagaaggcga
    12961 tgacaaagtt gttattactt tatctagtcc gtctccacaa ttcatctact accttgcatt
    13021 cactaatttc atgccacaaa aacaagaagt tgttgaaaaa tatggaaaag attacgcaac
    13081 tacttcaaaa aatacagttt actcaggacc atatactgtt gaaggttgga atggttcaaa
    13141 tggtactttc accctgaaga aaaacaaaaa ttattgggac gctaaaaatg taaaaacaaa
    13201 agaagttcgc atccagactg ttaaaaaacc agataccgcc gttcaaatgt ataaacgtgg
    13261 tgaattagat gcagctaata tctcaaatac ttctgctatt tatcaagcta ataaaaataa
    13321 taaagatgtc acagatgttc tagaagcgac cactgcctat atgcaatata atactactgg
    13381 ttctgtgaaa gggcttgata atgttaagat tcgtcgcgcc ttaaacttag caactaaccg
    13441 taaaggagtt gttcaagcag ccgttgatac aggctcaaaa ccggcaattg cttttgcacc
    13501 gactggttta gccaaaacac cagatggaac tgatttggca aaatatgttg ccccaggtta
    13561 tgaatataat aaaactgaag cagcaaaact ctttaaagaa ggtttggctg aatcaggctt
    13621 gactaagcta aaattaacaa ttacagcaga tgctgatgtt cctgctgcca aaaactctgt
    13681 tgactatatc aagtctactt gggaagctgc tcttccagga cttactgttg aagaaaaatt
    13741 tgtaaccttt aaacaacgct tagaagacag tagaaaacaa aactttgata tcgtagtttc
    13801 tgtttggggt ggggattatc cagaagggtc aactttctac ggcctcttta agtcagattc
    13861 acaaaataac gatggaaaat ttgctaacaa ggactatgac gctgcttata acaaggcaat
    13921 ttccgaagat gccttaaaac cagaagaatc agcaaaggac tataaagaag cagagaaaat
    13981 tctatttgag caaggcgctt ataacccact ctacttccgt agtggtaaag gtttacaaaa
    14041 tccaaaatta aaaggtgtta ttcgtaatac tacaggtttg tcaatagact ttacacatgc
    14101 ttataaaaaa taattaaatt actttacact aaacctttgc aagaattggt aattgccagt
    14161 tcttgctttt ggttaagtaa agaaagtgaa agagtttgct atttttagca attctaagaa
    14221 aaggaaatga tatgattaaa tatattttaa agcgtgttgc tattttgtta gtaacgctat
    14281 gggttgtcat aaccctatcg ttctttctga tgcagatcct tccaggaaca ccctacaata
    14341 atccaaaact cactgaagaa atgatcgcat tacttaataa acagtatggt ttagacaagc
    14401 ctgtatggca acaatatttg acttaccttt ggaatgtact tcatggtgac tttggaacga
    14461 gttatcaatc agtgaatcag ccagtatcac gtatgattag cttgagatta ggagtttcag
    14521 tacatctagg tgttcaggct ctcgtatttg gtgttttagg cggtattttg gtaggagcta
    14581 tttcggcacg tcataagaat gataaagtcg atggtatctt aagtgttatt gcaacgctag
    14641 ggatttctat gccttcattt atcattggta ttttattatt agactatttt ggatttaagt
    14701 ggaacttgtt gcctttatca ggttggggga ctttctctca aacaatttta ccttctttag
    14761 ctctaggact ccctacatta gcatctgttt cacgcttctt ccgtagtgaa atgattgaaa
    14821 ctttaaattc tgattatgtg caactagctc gttcaaaagg tatgacaatt cgtcaagtaa
    14881 ctcgaaaaca tgcttaccgt aattctatga ttccaatttt aactctaatc ggccctttgg
    14941 cggcgggtct gttaactggt tctgcactaa ttgaacagat cttttcgatt ccaggaattg
    15001 ggcagcagtt tgttacttcg attccaacaa aagactatcc tgttattatg gggacaacca
    15061 ttgtttatgc agtaatgttg atggttgcta tcttgataac agacgttgtt attagtatcg
    15121 tagacccacg cgttcgcttg cagtaaggag atgttatggc agataaaaac agaacattta
    15181 aacttgtagg tgcaggatct tctagcacac aagaaaaaat tgaaaagcct gctctttcgt
    15241 ttatgcaaga tgcgtggcgt cgcttgaaaa aaaacaagtt agcagtggtt tcactctatt
    15301 tattagctct tttacttact ttttcgttag cctcaaattt atttgtaact cagaaggatg
    15361 ctaatgggtt tgattcaaaa aaagtaacga catatcgcaa cttaccacct aaattgagtt
    15421 caaaccttcc tttttggaat ggtagcatta agtacgctgg aaatacagaa tctacagacg
    15481 cttataagtc tcaaaatgtc cctgaaaaag ttaagtatgc tttaggtaca gattctctag
    15541 ggagaagtgt tgctaagcgc atcatcgtag gtattcgaat ttccttactt gtagctattg
    15601 cagctacatt cattgattta attatcggtg ttacatacgg tcttgtttca ggttttgctg
    15661 gaggacgcct tgatacatta atgcaacgta tcgtagaggt aatttcatca attccaaact
    15721 tagttatcgt tacaatgtta ggtttggtcc ttggtaacgg tattacggct attattatct
    15781 ctattgcttt taccggatgg acttctatgt ctcgtcaggt aaggaattta accctatcat
    15841 atcgagaaag agagtttgtg cttgctgcac gttcacttgg ggaaagtccg attaaaatag
    15901 ctttcaaaca tattttacct aacatttctg gtattatcat tgtacaaatt atgatgacaa
    15961 tcccaagtgc tattatgtat gaagcagtac tttcagcgat caatttaggt gttaaaccac
    16021 cgacagcttc attaggatca ttgatttcag atgcacaaga aaatttacaa tattatccat
    16081 atcaagttat cttgccagct ttagcattgg taatgatttc attggcattt attttgcttg
    16141 gtgatgggtt gcgagatgct tttgatccaa aatctagcga tgaataagga ggtataatca
    16201 tggaaaaaga aactatttta agtgttaata atcttcatgt tgacttccac acatatgctg
    16261 gagaagtaaa agcaattcgt gatgtcaact ttgaattaaa aaaaggtgag actcttgcaa
    16321 tcgttggtga atctggttca ggaaaatctg taactaccag aactttaatt ggtttaaatg
    16381 ctaaaaattc agagatatcg gggaatgttc aatttaaggg gcgtaacctt gttgaactat
    16441 cagaagaaga gtggactaag gtacgcggga atgagatttc tatgattttc caagacccta
    16501 tgactagttt ggatccaacg atgaaaattg gtatgcaaat agcggagcca atgatgattc
    16561 atcaaaaaat ttcaaaaaag gatgctttaa aattagctct tgagctaatg aaagatgtag
    16621 gtatcccaaa tgctgaagag catattaatg attacccaca tcagtggtct ggaggaatgc
    16681 gccaacgtgc ggttatagcg attgctttag ctgctgatcc tgaaattctt attgctgatg
    16741 aaccaacgac tgctttagat gtaacaattc aagcacaaat tttgaactta atgaaaaaaa
    16801 ttcaagcaga acgagactcc tccattgttt tcataactca tgacttaggg gtcgttgcag
    16861 ggatggcaga ccgtgtagca gttatgtatg cagggaaaat tgttgaattt gggactgttg
    16921 atgaagtctt ttataatcca caacatccat atacttgggg attgttgaat tcaatgccga
    16981 caaccgacac agaatcaggt agtttagagt caattccggg aacaccacca gatttgttga
    17041 atccacctaa aggagatgca tttgcagctc gtaatgaatt tgcattagat attgatcatg
    17101 aagaagaacc accgtacttc aaagtttcag aaacacattt cgctgctaca tggcttttag
    17161 atgaaagatc acctaaggtg cttcctccat taccgatcca aaaacgttgg gaaaaatgga
    17221 acgagattga agggaggaaa gcctaatgac tgaaaatcga aaaaaattag ttgaagtcaa
    17281 aaatgtttct ttgaccttca ataaaggaaa agctaatgaa gtgagagcaa ttgataatgt
    17341 tagttttgac atttatgaag gtgaagtgtt tggattagtt ggggaatctg gatcaggaaa
    17401 gacaaccgtt ggacgttcaa ttttaaaact ttatgatatt tctgatggag aaattacctt
    17461 taatggagaa gtgatctcac atctgaaagg taaagcatta catagttttc gtaaagacgc
    17521 tcagatgatt tttcaggatc ctcaggctag cttaaacggc cgtatgaaga ttcgtgatat
    17581 cgtagcagaa ggtttagata tacataaatt agctaaatca aaatcagatc gtgatagtaa
    17641 agttcaggcc ctattagatc ttgtagggtt aaataaagat catttaacac gttatccgca
    17701 tgaattttca ggtggacaac gacaacgtat cgggattgca cgtgctttag cagtagagcc
    17761 taaatttatc attgctgatg aaccaatatc tgccttagat gtttcaatcc aagcacaagt
    17821 tgttaattta atgcaaaaat tgcaaagaga acaaggattg acttatttgt ttatcgcaca
    17881 tgatttgtct atggtaaaat acatttcaga tcgtatcggt gttatgcatt ggggaaaact
    17941 gttagaagtt gggacatctg atgatgttta taacaatcca atccacccct atacgaagag
    18001 tctattatca gctattccag aaccagatcc ggagagtgaa cgtcaacgtg ttcaccagcc
    18061 gtataatcca gctattgagc aagacggaca agaacgtcaa atgcacgaga ttaccccttg
    18121 tcattttgtt ttatctacac cccaagaagc tgaagaatat aaaaaacaaa tattataaaa
    18181 gatggctagc ccatctttta tttttttact aataaaataa atttaggtat ggaattttaa
    18241 actctatgtt attggtgtta gccaacagga aact
//
LOCUS       DATRTN010000031        17898 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.031, whole genome shotgun sequence.
ACCESSION   DATRTN010000031 DATRTN010000000
VERSION     DATRTN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17898
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.031"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            143..985
                     /locus_tag="VBE22_001064"
     CDS             143..985
                     /locus_tag="VBE22_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO7202532.1"
                     /translation="MEQDMELMNAYRLYYDYAHLSLKEVVLAYMSEYGIEKNSVAFSY
                     KNLKTGQRDAMNDTQAMTAGSTYKLPLNMLVVDAVVKGKLSMEERFDITETDYEYIGE
                     HNNYVAAFSGAMSIDDMQKYSLVYSENTPAYALAERIGGMDSAYSKFGRYGQSKGDIK
                     NIQKNGNKVTTDYYIQVLDYLWKHRKKYDSLITYLEEAFPTDYYRALIPSDVVVAQKP
                     GYVREALNVGAIVKEEVPYIVAIYTAGLGGSTQEDSEINGVGLYQLEQLCFVINQWHR
                     VNMN"
     gene            complement(1040..1375)
                     /locus_tag="VBE22_001065"
     CDS             complement(1040..1375)
                     /locus_tag="VBE22_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7202533.1"
                     /translation="MTYLQYVILLVLWEEDHRHMKELSYALSLNSNTLTPVLKRMENR
                     GWIVREKPRDDKRQLIIHLSNKGIESQDAVVNAVQSCGYFFSEISPESYQKTVSALSS
                     LNQVIKSHL"
     gene            complement(1564..2592)
                     /gene="queA"
                     /locus_tag="VBE22_001066"
     CDS             complement(1564..2592)
                     /gene="queA"
                     /locus_tag="VBE22_001066"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEO7202534.1"
                     /translation="MNTNDFDFYLPEELIAQTPLEKRDASKLLVIDHKNKTMTDSHFD
                     HILDELKPGDALVMNNTRVLPARLYGEKPDTHGHIELLLLKNTEGDQWEVLAKPAKRL
                     RVGTKVSFGDGRLIATVTKELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     RDRYQTVYAKENGSAAAPTAGLHFTKELLEKIETKGVKLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSKEAADTLNAVKESGGRIVAVGTTSIRTLETIGSKFNGELKADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYNHAVEERYRFFS
                     FGDAMFVK"
     gene            2745..3425
                     /locus_tag="VBE22_001067"
     CDS             2745..3425
                     /locus_tag="VBE22_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEO7202535.1"
                     /translation="MKEAKNFSQQLNILRAGVLGANDGIISVAGVVIGVASATHNLWI
                     IFLSAASAILAGAFSMAGGEYVSVSTQKDTEQAAVAREEKLLENNPELAKKSLVDIYL
                     AKGESHEHAQWLVDKAFSKNAIEHLVEEKYGIEFGEYTSPWHAAISSFIAFAIGSIFP
                     TITILLLPFSVRIVGTVIIVIVSLLSTGYVSAKLGQAPTVPAMRRNVMIGCLTMLATY
                     VIGQLFSI"
     gene            3576..4277
                     /locus_tag="VBE22_001068"
     CDS             3576..4277
                     /locus_tag="VBE22_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEO7202536.1"
                     /translation="MRVITVKNDIEGGKIAFTLLEEKMKAGAQTLGLATGSSPITFYE
                     EIVKSNLDFSNMVSINLDEYVGIAASNDQSYSYFMHKHLFDAKPFKENNLPNGLAKDL
                     KEEIKRYDAVINANPIDFQILGIGRNGHIGFNEPGTPFDITTHVVDLAPSTIEANSRF
                     FNSIDDVPKQALSMGIGSIMKSKTIVLVAYGIEKAEAIASMIKGPITEDMPASILQKH
                     DDVVIIVDEAAASKL"
     gene            complement(4349..5305)
                     /locus_tag="VBE22_001069"
     CDS             complement(4349..5305)
                     /locus_tag="VBE22_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO7202537.1"
                     /translation="MGLLVDGKWVDQWYDTASTGGKFVRTVTQFRHWVTKDGSAGPSG
                     DAGFKAESGRYHLYVSLACPWASRVLIMRKLKNLESHISISIVNPLMLENGWTFQEYK
                     GVIPDMINQSQYLYQIYQASQSDYTGRVTVPVLWDKKCHTIVNNESSEIMRMLNTAFN
                     HITGNTDDYYPDSLQGQIDEMNNFIYPKINNGVYKAGFATSQNVYQKEVETLFTALDQ
                     LEKHLSDNHYLVGEQFTEADIRLFTTLVRFDTVYYGHFKCNLKALHDYPHLWHYTKRI
                     YNLPGIAETVNFDHIKKHYYGSHKTINPTGIIPAGPNLDWTI"
     gene            5401..6120
                     /locus_tag="VBE22_001070"
     CDS             5401..6120
                     /locus_tag="VBE22_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO7202538.1"
                     /translation="MRLDKLLGQAGFGSRNQVKKLIRSRQVSVDGQIVTKDNVIVDSG
                     LQSIFVGKERVCLKESSYYLLYKPSGVVSAVRDSEHKTVIDLISEKDRVEGLYPIGRL
                     DRDTEGLLIITNNGPLGYRMLHPKHHVAKTYYVEVNGFLERDAITFFEEGVVFDDGTK
                     CKPAELTIDTANNDKSTARITITEGKFHQVKKMFLAYGVKVIYLRRISFGDLRLDMNL
                     KPGQYRRLRDSEAAILKRYLD"
     gene            6193..7344
                     /locus_tag="VBE22_001071"
     CDS             6193..7344
                     /locus_tag="VBE22_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7202539.1"
                     /translation="MNSKLSLKLTILSISIFLMSHLAIAPAIPKLYAFYHTQNPHIGL
                     ASVESLVTIPAMMITIFVILSNFVVTKLGKKNTVLLGLCLILISGFISFFTSNFSLAM
                     ASRLLLGIGIGLYNSLSISIITDLYEADERASMIGLRTASLNIGKALTTFIVGLVLAI
                     GVNYIYLVYLLVIPVFFFFWKNVPEVENQTHTLKASTTFDTKAALLMFITFLVGIAYI
                     GATVKIPTLLVTKYHYATSFSSNMLTLLAFSGILVGSIFGKLVKVFQEKTLLIMILAM
                     GIGNVLFALANNQIIFIVASILIGASFVGTMSSVFFYISKNYAKEHNNFITSLALTAG
                     NIGVILTPLILTKLPSQLHLEPFMTPFLITSGLMVINVFVYLVLMSKNK"
     gene            7396..8343
                     /locus_tag="VBE22_001072"
     CDS             7396..8343
                     /locus_tag="VBE22_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEO7202540.1"
                     /translation="MLIAKDRQGNLINLLESHPGKGQYFCPTCCSAVRLKAGRIMRRH
                     FAHISLKNCQFYHENESNEHLQLKAKLYMSLSRENETMLEHHLPEINQIADLFVNETL
                     ALEVQCSRLSEQRLRERTKAYLQADFQVRWLLGEKLWLKDRLTNLHKQFLQFSQSIGF
                     HIWELDLRLEVLRLKYLIYEDLRGHVYYLSKTCPLSGDVLAFLKWPYQSKNLNFYKVK
                     QDRNIRDYVRQQLRYGNQFWLRKQEKAYLSGQNLLTQELMMFFPQIQPPRVDTDFCQI
                     TNSLTSFYQNFTNYYQKNKNNLDQTLYPPVFYDKIVTKN"
     gene            8359..10164
                     /gene="pepF"
                     /locus_tag="VBE22_001073"
     CDS             8359..10164
                     /gene="pepF"
                     /locus_tag="VBE22_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO7202541.1"
                     /translation="MSDNRSHIEEKYQWDLTTVFATDELWETEVVELTQAIDNAKGFS
                     GHLLDSSQSLLEITEVELDLSRRLEKVYVYASMKNDQDTTVAKYQEFQAKATALYAKF
                     SETFSFYEPELLQLSESDYQSFLLEMPDLQKYDHFFEKIFANKPHVLSQNEEELLAGA
                     SEIFGAAGETFEILDNADMVFPVVKNAKGEEVELTHGNFISLMESSDRTVRKEAYQAM
                     YSTYEQFQHTYAKTLQTNVKSQNFKARVHHYQSARQSALSANFIPEEVYETLIKTVNH
                     HLPLLHRYMKLRQKVLGLDDLKMYDVYTPLSQMDMSFTYDEALKKSEEVLAIFGEVYS
                     ERVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRENQPYVYGDYSIFLAEIASTTNENILTETLLKEVKDDKNRFAILNHYLDGFK
                     GTIFRQTQFAEFEHAIHVADQEGQVLTSEYLNNLYAELNEKYYGLTKEDNHFIQYEWA
                     RIPHFYYNYYVFQYATGFAAANYLAERIVNGNPEDKEAYLNYLKAGNSDYPLNVIAKA
                     GVDMTSADYLDAAFRVFEERLVELENLVAKGVHND"
     gene            10359..10985
                     /locus_tag="VBE22_001074"
     CDS             10359..10985
                     /locus_tag="VBE22_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEO7202542.1"
                     /translation="MKKLTFIWDLDGTLIDSYVPIMEALEETYRHFGLIFDKELIHEY
                     ILQESVGQLLVNLSEEEQIPHEKLKAYFTKEQESRDSKIHLMPYAKEILEWTKEQDIP
                     NFMYTHKGASTHSVLETLQISHYFDEILTGVSGFERKPHPQGINYLVKRYSLDKSMTY
                     YIGDRPLDLEVAQNAGIKSINLRLENSKENYNISSLKDIISLDFTRLD"
     gene            11059..11766
                     /locus_tag="VBE22_001075"
     CDS             11059..11766
                     /locus_tag="VBE22_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEO7202543.1"
                     /translation="MVQSYSKNANHNMRRPVVKEEIVQYMRQHQKQNNGCLAELEAFA
                     KQENIPIIPHETATYFRFLMQTLQPKHILEIGTAIGFSALLMAENAPEAKITTIDRNE
                     EMIVLAKENFAKYDNHNQITLLEGDAVDVLQTLDKSYDFVFMDSAKSKYIVFLPQVLK
                     HLDVGGVVVLDDIFQGGDIAKPIDEVRRGQRTIYRGLQRLFDSTLRHPDLTATLVPLG
                     DGLLMIRKNAEHIVLED"
     gene            11827..12756
                     /gene="prsA"
                     /locus_tag="VBE22_001076"
     CDS             11827..12756
                     /gene="prsA"
                     /locus_tag="VBE22_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEO7202544.1"
                     /translation="MKTRSKLAAGFLTLMSVATLAACSGKTSNGTNVVTMKGDTITVS
                     DFYDQVKTSKAAQQSMLTLILSRVFDTQYGDKVSDKKVSEAYNKTAKGYGNSFSSALS
                     QAGLTPEGYKQQIRTTMLVEYAVKEAAKKELTEANYKEAYKNYTPETSVQVIKLDAED
                     KAKSVLKDVKADGADFAKIAKEKTTATDKKVEYKFDSAGTSLPKEVMSAAFKLDKNGV
                     SDVVSTVDSTTYKTSYYIIKVTDKTEKKSDWKSYKNRLKEVILKDKTSDRSFQNKVIS
                     KALEKANVKIKDKAFAGILSQYATTSGSSSLKK"
     gene            12998..13483
                     /locus_tag="VBE22_001077"
     CDS             12998..13483
                     /locus_tag="VBE22_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEO7202545.1"
                     /translation="MATFQIKEKMFSLGGKFTITDQTGLPCYHVEGSLFPLPKTFKVF
                     DEEGHLISQIEKKVLSFLPKFNVTLANGNHFTIKKDFSFLKPHYTIEDLDMEVKGNFW
                     DMDFQLLKDNQVIANISQQWFRMTSTYQVEVYNETYNDLTISLVIAIDYVKELEKNAS
                     N"
     gene            13499..16117
                     /gene="alaS"
                     /locus_tag="VBE22_001078"
     CDS             13499..16117
                     /gene="alaS"
                     /locus_tag="VBE22_001078"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEO7202546.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPDNIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDLQKENVELKEKAAAAAAGDVFKDIQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKGLAPIVSGRGGGKPDMAMAGGS
                     DASKIAELLAAVAENL"
     gene            16199..16915
                     /locus_tag="VBE22_001079"
     CDS             16199..16915
                     /locus_tag="VBE22_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEO7202547.1"
                     /translation="MVGLGTVINVILIIVGGFVGLFLKNFLKESLQKSLMQAMGVAVL
                     FISISGVLEKMMLVEKSHLISNHTNMMIITLALGTVLGELLSLDSYIDKFGNYLKQKT
                     GSGNDIKFVEAFVTSTCTVCIGAMAVVGSIQDGIAADHSILFAKGMLDMIIIAIMTVS
                     LGKGALFSALPVALLQGSLTIVAFFMGSLLNPSSLDYLNLVGNMLIFCVGVNLLFNLN
                     IKVINMLPAIILAILWGSFI"
     gene            17003..17821
                     /locus_tag="VBE22_001080"
     CDS             17003..17821
                     /locus_tag="VBE22_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEO7202548.1"
                     /translation="MNLLFSIDDMYVDHFKVMLYSLVRQTKNRKLEIYVLQKTLLKRH
                     TELIQYTQNLEVGYHPIIVGTEVFAQAPTTDRYPDTIYYRLLAHKFLPETLDRILYLD
                     ADMLCLNDFSSLYDMELGDQLYAAASHNTDGKFLDYVNKLRLKNVELESSYFNTGVLL
                     MNLPAIRKVVHQQTILDYMMQNRGRLILPDQDILNGLYANLVKPIPDEIYNYDARYSL
                     IYQLKSRNEWDLEWVINHTVFLHFAGRDKPWKKDYRGRYSGLYKFMAKEALTKN"
BASE COUNT         5935 a         2895 c         3447 g         5621 t
ORIGIN      
        1 agtaatgttt aataagtcca gtaactctaa gttgaagcag acaaaagata gaagagtatt
       61 atctacttca accacaagga catccagtag ctcacatgat caaaaaattg tagaaaagtc
      121 tcttgaaaaa aagaaaatgg tcatggaaca ggatatggaa ttgatgaatg cctataggct
      181 ctattatgat tatgcccatt tgtccttgaa agaggttgtt ctggcctata tgtctgaata
      241 tggcattgaa aaaaatagtg ttgctttttc ttataaaaat ttaaaaacag gacaaaggga
      301 cgctatgaat gatactcagg ctatgacagc agggagcacc tataagttac ctcttaacat
      361 gttggtagta gatgcagtgg tcaagggcaa gttgtccatg gaagaacgct ttgatattac
      421 cgagactgac tatgaatata ttggtgaaca taataattat gttgctgctt tctcgggggc
      481 aatgtctatt gatgatatgc aaaaatactc tttagtttat tcagaaaata cacccgccta
      541 tgctttagct gagcgtattg gtggcatgga tagtgcttat tcaaagtttg gtcgttatgg
      601 ccaatctaaa ggagacatca aaaatataca aaaaaacggt aataaagtga caacagatta
      661 ctatattcaa gtgctagact atctctggaa acatcgtaaa aagtatgata gtttaattac
      721 ttatttagaa gaagcttttc caacagatta ttatcgtgct ttgataccat ctgatgttgt
      781 tgttgctcaa aagccagggt atgtccgtga ggcgttgaat gttggagcaa ttgtcaaaga
      841 agaagtgcct tatattgttg ctatttatac tgcaggttta ggtggctcta ctcaggaaga
      901 ttcagaaatt aatggagtgg gactatatca gctagaacaa ttatgttttg ttattaatca
      961 atggcatcgt gttaatatga attagaaata aatctatcaa aataataaaa acctcttaga
     1021 ttaactatct aagaggtttt tataaatgtg attttataac ttgattaagt gaagaaaggg
     1081 cagaaacagt tttttgataa gattcaggac taatttctga gaaaaaatac ccgcacgatt
     1141 gcaccgcatt gacaacggca tcttgtgact caataccttt gttacttaga tgaataatga
     1201 gttgcctttt atcatctctt ggtttttctc gtacaatcca cccacgattt tccatacgct
     1261 ttaaaactgg agttagggta ttcgaattaa gagatagtgc ataactaagt tccttcatat
     1321 gccgatgatc ctcttcccaa aggactaaca agataacgta ttggagataa gtcaattgaa
     1381 attcatccaa agctttttgg taaaatttat tgaaaagtcg atgtgtttca ctaaccgtaa
     1441 aacataatcg ctcgtctaaa atacttcgat tactcattat catattgtag cattttggag
     1501 gcgttatagt atgtcaaccg tgttaaaaaa tgataaaact tttttctaat tgaaagccta
     1561 ttcttactta acaaacatag catcaccaaa gctgaagaaa cgatatcgtt cttcaactgc
     1621 atggttgtaa gcttctagta caaaatcacg tccagcaaat gctgatacta acatgacaag
     1681 agtagacttt ggtaagtgaa agttggttga aaaagcgtct acaaccttga actgataacc
     1741 aggcttgata aaaatatttg tccaccctga gtcggctttt aattcaccat tgaatttaga
     1801 accaattgtc tctaatgtgc gaatagaagt tgtaccaaca gctacaatac gtccaccaga
     1861 ttctttaact gcattgagag tatcagcagc ttctttagat aattggtaaa actcagaatg
     1921 catttcgtgt tcatcaagat tatcaactga aacaggacgg aaagttccaa gtccaacgtg
     1981 aagcgtcaaa tagacaagct tcacaccttt tgtttcaatt ttttccaata gttcttttgt
     2041 aaaatgaaga ccggctgttg gtgcagcagc agaaccattt tcttttgcat acacagtctg
     2101 atagcggtca cgatcttcta gtttttcatg aatatatggc ggaagcggca tttcgcccaa
     2161 actttcaagg acttctagaa aaataccatc atatgagaat tctacaatac gcccaccatg
     2221 ttctaattcc tttgtgactg ttgctattaa ccgaccatca ccaaatgaaa ctttggtccc
     2281 tacgcgtaag cgtttagcgg gtttagcaag cacttcccat tgatcgccct ctgtattttt
     2341 gagaagtaat aactctatat ggccgtgtgt atctggcttt tcaccataaa ggcgagcggg
     2401 aagaacacgt gtgttattca ttactaaagc gtcaccaggc ttgagttcat ctaagatatg
     2461 atcgaaatgg ctatctgtca tcgtcttatt cttatgatcg atgactaata gtttagaagc
     2521 gtctcttttt tcaagtggtg tttgggcaat gagttcttct ggtaaataaa aatcaaaatc
     2581 gtttgtattc atgatttctc ctatctataa gtaagctttt ctattatatc acgttcggta
     2641 aaaatgaccc aactaatctt tttatttaga atgattataa aaaataattg taatcattct
     2701 aaataagtga tataataatt gtcagaaagt gagaagtaga gaagatgaaa gaagcaaaaa
     2761 atttttccca acaattgaat attttaagag ctggtgttct tggtgctaat gatggtatca
     2821 tctctgtagc tggtgttgtt attggtgttg cgagtgcaac tcataacctt tggattattt
     2881 ttttatcagc agcttccgcg attttagctg gtgcattttc tatggctggc ggggagtatg
     2941 ttagtgtttc tactcaaaaa gatactgaac aagcagcagt tgcgcgtgaa gagaaactac
     3001 tagaaaataa tccagaactt gccaaaaaat ccttagttga tatctactta gcaaaaggag
     3061 aaagtcacga acatgctcaa tggctagttg acaaggcttt ttcaaagaat gctattgagc
     3121 atttagttga agaaaaatat ggtattgagt tcggtgaata tacaagcccc tggcatgctg
     3181 ctatatctag ttttatagct tttgccatcg gatcaatttt tccaactata actattctgt
     3241 tattgccatt ttcggtacga attgtaggaa cggtaataat tgttattgtt tcactattat
     3301 caactggtta tgtaagtgct aagttaggtc aagcccctac agtacctgcc atgcgtcgaa
     3361 atgtgatgat tggttgtttg acaatgttgg caacctatgt cattggacaa ttattttcaa
     3421 tataaaatat taatcgtgga gttattgtct aaactagaat ggacagtaat tctttttagt
     3481 tttataacga aattaagaag tttttattga caaaaattgg tatataccat ataatataat
     3541 cataaaggtc tataccaaat aaaaggagag taaacatgag agttattaca gttaagaacg
     3601 acattgaggg gggaaaaatt gcatttactc ttttagaaga gaaaatgaaa gctggtgctc
     3661 aaactttagg attggcaaca ggaagtagcc caatcacctt ttacgaagag attgtaaaaa
     3721 gtaatcttga tttttcaaat atggtttcta ttaaccttga tgaatatgtt ggaattgccg
     3781 caagtaatga tcaaagttac tcttatttta tgcataaaca tctttttgat gctaaacctt
     3841 tcaaggaaaa taaccttcca aacggcttgg ctaaagattt gaaagaagaa ataaaacgat
     3901 acgatgctgt cataaatgct aatccaattg attttcaaat ccttggaatt ggtcgtaacg
     3961 gtcatattgg ttttaatgaa ccaggtacac cttttgatat tacaacacat gtggttgatt
     4021 tagctccaag tacaattgaa gctaatagtc gtttctttaa tagtattgat gatgtaccaa
     4081 agcaagcctt atcaatgggt ataggctcta ttatgaaatc aaaaacaatc gtattagtgg
     4141 cgtatggtat tgaaaaagca gaagcgattg caagtatgat taaaggacct atcacagaag
     4201 atatgccagc aagtatcctc caaaaacatg atgatgttgt gattattgtg gatgaagcag
     4261 cggcatcaaa gttgtaaagt aaataataaa aaagtaagga tgaatctcaa tgagaaaaaa
     4321 ttcattctta cttttttatt atgctttatt aaattgtcca gtctaagtta ggtccagctg
     4381 gaataatacc agtagggtta atggttttat ggctaccgta atagtgtttt ttgatatgat
     4441 caaaattaac tgtctcagca attccaggta ggttgtaaat acgttttgta taatgccata
     4501 aatgtgggta atcatgtagc gcttttaaat tacatttaaa atgaccataa taaacggtgt
     4561 caaagcgaac taaagttgtg aaaaggcgaa tatcagcttc ggtaaattgt tctccaacaa
     4621 gataatggtt atcagataag tgtttttcta actgatctaa agctgtgaat aaggtttcaa
     4681 cttccttttg ataaacgttc tgagaagtag caaaaccagc tttatagaca ccattattta
     4741 tttttggata aataaaatta ttcatttcgt caatttgtcc ttgtaaggaa tcgggatagt
     4801 aatcgtcagt atttccagta atatgattaa atgcagtatt taacatacgc ataatttcag
     4861 atgattcatt gttaacgatt gtatggcatt ttttatccca aagaacaggg actgtcacgc
     4921 gtcctgtata atcactttga cttgcttgat agatttggta aaggtattgg ctttggttaa
     4981 tcatatcagg aataacgcct ttatattctt gaaaagtcca accattctcc aacattaaag
     5041 ggttaacaat ggagatagag atatgagatt ctagattttt aagctttctc atgattaata
     5101 cacgtgatgc ccaagggcag gctagtgaaa cgtagagatg atatcgtcca gattctgctt
     5161 taaaacctgc atccccacta ggccctgctg aaccatcttt agtaacccaa tgacggaatt
     5221 gtgtaacggt tcggacgaat ttaccaccag ttgatgcagt atcataccat tgatcaaccc
     5281 atttgccatc tactaaaagt cccatgataa tctcctttgt ttttctatta ctaattagta
     5341 gtataaagat tttaaaagga attagcaaat gtttaactta cagtatggta aactaagggt
     5401 atgcgtttag ataaattatt aggtcaggca ggatttgggt ctagaaatca ggttaagaaa
     5461 ttaattcgtt ctaggcaagt ttctgtagat ggtcaaattg ttacaaagga taatgttatt
     5521 gtagatagtg gtctacaatc gatttttgta ggaaaagaga gagtctgcct aaaagaaagt
     5581 tcttattacc ttctttataa gccatcagga gttgtttcag cagttagaga ttcagaacat
     5641 aaaacagtca tagacttaat tagcgaaaaa gatagggtgg agggattgta tccgattggt
     5701 cgtctagatc gtgatacgga agggctttta ataatcacga ataatggtcc actaggttac
     5761 cgaatgttac atccaaaaca ccacgttgca aaaacgtatt atgttgaagt aaatggtttc
     5821 ttggaaagag atgctattac cttttttgaa gaaggagttg tatttgacga tggaacaaag
     5881 tgtaaaccag ctgaattaac aattgatacg gctaataatg acaaaagtac cgcaagaata
     5941 actatcacag aagggaaatt tcaccaagta aaaaagatgt ttcttgcata tggcgttaaa
     6001 gttatatact tgaggcgtat ttcttttggt gacttgaggc ttgatatgaa tttgaaacca
     6061 ggtcagtatc gtagattgag agattcagag gcagctatct taaaaaggta tttagattag
     6121 tgaaacgact cacttgacct tgcttagtat tcttcatata cttataagtg attattttaa
     6181 aggagaaaac cattgaattc aaaactttca ttgaaattga ctattttatc gatttctatc
     6241 tttttgatgt ctcacttagc aattgctcct gcaattccta aattgtatgc cttttaccat
     6301 actcaaaatc ctcatattgg actagcttca gtagagagtt tagttactat tccagctatg
     6361 atgattacta tttttgttat tttgagtaat tttgtagtca caaaacttgg caaaaaaaac
     6421 acggtcttac ttggtttatg tttaatctta atatctgggt ttatctcatt ttttacaagt
     6481 aatttttcac ttgcgatggc tagtcgacta cttttgggta ttggtattgg tctctataat
     6541 tcgctatcaa ttagtatcat aacggattta tatgaggctg atgagagagc cagtatgatt
     6601 ggtcttagaa cagcatcact taatatcggt aaagccttaa ctacatttat tgttggattg
     6661 gttttagcta ttggtgtgaa ttatatttat ttggtttacc ttctggttat tcctgttttc
     6721 tttttctttt ggaaaaatgt tccagaggtt gaaaatcaaa cacataccct taaagctagt
     6781 acaacatttg atactaaagc ggctttgttg atgttcatca cgttcttagt aggtattgct
     6841 tatataggag caactgtcaa aattccaacc ttactagtga caaaatatca ctacgcgacg
     6901 agcttctcta gtaatatgtt aactctcctt gcttttagtg gtattttagt tggatctata
     6961 tttggaaaat tagttaaagt attccaagag aagacgctat taatcatgat tctagctatg
     7021 ggaataggta atgtgctatt tgctttagct aataatcaga tcatttttat cgtagctagt
     7081 attttaatag gagctagttt tgttggcacc atgtcttctg tattctttta tatttcaaaa
     7141 aactatgcaa aagaacataa taactttatc acaagccttg ccttaacagc tggtaatata
     7201 ggagttattt tgacaccgct tattttaacg aaacttccaa gccagctaca tctggaacct
     7261 tttatgacac catttttaat aacaagtggg ctgatggtaa ttaatgtctt tgtttaccta
     7321 gttttaatgt caaaaaataa ataaaaagtt atccgaaagg ataacttttt tcgaatagta
     7381 aaagtaggag aaaaaatgtt aattgctaaa gacagacaag gaaatttaat taatctactc
     7441 gagtctcatc cgggaaaggg acagtatttt tgtccaactt gttgttcagc tgtaaggtta
     7501 aaagcaggtc gaatcatgag aagacacttt gcacatatta gtctaaaaaa ttgtcagttt
     7561 tatcatgaaa atgagtctaa cgagcacttg caattaaaag caaagttata tatgagttta
     7621 agtagagaga atgaaactat gctcgagcat catttgccag aaattaatca gattgcagat
     7681 ttatttgtga atgagacatt ggctttagaa gttcagtgta gccgactatc agagcagcga
     7741 ctcagggagc gaacaaaagc atatctacag gcagactttc aagtgagatg gttactaggg
     7801 gaaaaactct ggttaaagga tcgtttaaca aatctacata aacaattttt acaattttct
     7861 caaagtatcg gatttcatat ttgggagtta gatttgaggt tagaagtact gaggctaaaa
     7921 tatttgattt acgaagattt acgcggacat gtctattact taagtaaaac ttgccctctt
     7981 agtggagacg ttctagcatt tttgaagtgg ccataccaat caaaaaatct aaatttttat
     8041 aaggttaaac aagacagaaa tatcagagat tatgtgcgtc agcagttacg ctatggcaat
     8101 caattttggt tacgaaaaca agaaaaggcc tacctttctg gtcagaatct attaacccaa
     8161 gagctaatga tgttcttccc tcaaatacag ccaccacgtg tagatactga tttttgtcaa
     8221 ataacaaatt ctctaacatc gttttatcag aattttacaa attattatca aaaaaataag
     8281 aataacttgg accaaacact ttatccccct gtattttatg ataaaatagt cactaagaat
     8341 taaactggag gatacaaaat gtcagacaat cgctcacata ttgaagaaaa ataccaatgg
     8401 gatttaacta ctgtctttgc gactgatgag ttatgggaaa ctgaagtggt agagcttact
     8461 caagccatcg ataacgcaaa gggtttttca ggtcatctgt tggactcaag ccaatcttta
     8521 ttggagatca cggaggttga actagattta tctcgccgtt tagaaaaagt ttatgtctat
     8581 gcttcaatga aaaatgacca agacaccact gttgcgaaat atcaagaatt tcaagcgaaa
     8641 gcgacagcgc tctatgcaaa atttagtgaa acattttcgt tttacgagcc agaactattg
     8701 caactttcag agtctgatta ccagtcattc cttttagaaa tgcctgactt gcaaaaatat
     8761 gatcatttct ttgagaaaat atttgcaaat aaacctcatg tcttatctca aaacgaagaa
     8821 gaattgttag ctggtgctag tgagattttt ggagcagctg gtgagacatt tgaaatcctt
     8881 gataatgcag atatggtctt cccagttgta aaaaatgcaa aaggtgagga agttgagctt
     8941 acacatggta atttcatttc ccttatggaa tctagcgata ggacggttcg aaaagaagca
     9001 tatcaagcta tgtatagcac atatgaacaa ttccagcata cttacgctaa aacattacaa
     9061 acaaatgtga aatctcaaaa ttttaaagct cgtgtgcatc attatcaatc agcacgccaa
     9121 tcagctctat ctgccaattt tattccagaa gaggtctacg aaactctaat taagacagtt
     9181 aatcatcatt taccgctctt acatcgttat atgaagttgc gtcaaaaagt tcttgggcta
     9241 gatgatttga agatgtatga cgtatataca cccctttctc aaatggatat gagttttact
     9301 tatgatgaag ctctaaaaaa atcagaggag gttctggcta tttttggcga agtatattct
     9361 gaacgtgttc atcgtgcttt tactgaacgt tggattgatg tccatgttaa caaaggaaag
     9421 aggtcaggcg cttattcagg tggatcttat gacactaatg ctttcatgct tcttaactgg
     9481 caagatactt tagacaatct ttatacgctg gtgcatgaaa ctggacatag tctacactcg
     9541 acgtttactc gtgaaaatca accctatgtt tatggtgatt attctatttt cctagctgaa
     9601 attgcatcaa caacaaatga aaatatatta acagaaaccc ttttgaaaga agttaaagat
     9661 gataaaaatc gctttgcaat tctcaatcat tatttggacg gtttcaaagg aacaattttt
     9721 agacaaacac agttcgctga attcgaacat gccattcatg tagctgatca ggaggggcaa
     9781 gtactaacta gcgaatactt aaacaacctt tacgctgagc ttaatgaaaa atattatggt
     9841 ttgactaaag aagataatca ttttattcaa tatgaatggg caagaattcc acacttttac
     9901 tacaattatt atgttttcca atatgcaacc ggcttcgcag cagcaaatta tttagcagag
     9961 cgtattgtaa acggtaatcc ggaggataaa gaggcctacc taaattacct taaagctggg
    10021 aattcagatt accctctaaa cgtcattgct aaagccggtg ttgatatgac tagtgcagac
    10081 tatctggatg cagcttttag agtatttgaa gagcgtttag tggaactgga aaatcttgta
    10141 gcaaaaggcg tccataatga ttaagttgaa aatatagcta ttgaagtaaa accagtcaac
    10201 tagactggtt tttgatagtg atcagttatt ttatagtttg ttttgagagt ggttttggta
    10261 aagttagtta gtggaaaact caataggtgg aaatacctac ccaagctatg atttttatga
    10321 gaccaaaact gctattgtca aaaaagaagg gaaataggat gaaaaagctt acttttattt
    10381 gggatttaga tgggacatta atagattcgt atgtaccaat tatggaagct cttgaagaaa
    10441 cctatcgtca ttttggctta atatttgata aagaattaat ccatgaatat attttacagg
    10501 aatcagtggg gcaattattg gtaaaccttt cagaggaaga gcaaatacct catgaaaaac
    10561 tgaaagcata ttttacaaaa gaacaagaaa gtcgagattc taaaatacat ttaatgccat
    10621 atgcaaaaga gattttagaa tggaccaaag aacaagatat ccccaatttt atgtatacac
    10681 ataaaggagc aagtacgcat tcagtgttgg aaaccttgca gatctctcat tattttgatg
    10741 aaattttaac tggtgtttct ggattcgagc gaaaaccaca tccacaaggg attaattatt
    10801 tagttaaacg atattcttta gataaatcaa tgacttatta cataggagat cgtcccctag
    10861 atttggaggt tgctcaaaat gctggtataa aatccataaa cttaaggtta gagaattcca
    10921 aagaaaacta taatatttca agtctcaagg atataatatc acttgatttc actcgtttgg
    10981 attaaatcat tctcagagct agacattttt tgctataata aaaaagttat tatttaaaat
    11041 agaaaagagg agccaaaagt ggttcaatct tacagtaaaa atgctaatca taatatgcgc
    11101 cgtccagtag ttaaagaaga aattgttcaa tatatgcgtc agcaccaaaa acaaaataat
    11161 ggctgtttag cagagttaga agcatttgct aaacaggaaa atatccctat tatacctcat
    11221 gaaacagcga cttattttcg ttttttgatg caaacattac aacctaagca cattttagaa
    11281 attggaacag ccattggttt ttcggccctt ttgatggcag aaaatgctcc tgaagcaaag
    11341 ataacaacga tcgatcgtaa tgaagagatg attgtcttgg ctaaggaaaa ctttgcaaaa
    11401 tatgataatc acaatcagat aacccttttg gaaggcgatg ctgtggatgt gttacaaaca
    11461 ttggataaat cttacgattt tgtatttatg gattctgcta agtctaaata tatcgttttt
    11521 ttacctcaag tattaaaaca tttagacgtt ggaggagtag ttgttttaga tgatattttc
    11581 caaggtggag atattgcaaa acctatcgat gaagtgagaa gaggtcagcg aactatctac
    11641 cgaggtctgc aacgtttatt tgactcgact ttgcgacatc ctgatttaac tgctactttg
    11701 gtacctttgg gagatggttt attgatgatt cgtaaaaatg cagagcatat tgtactagaa
    11761 gattaaggca tttttaagaa attatgatat aataatggag ttaatgacaa aaaggagtta
    11821 taaatcatga aaacaagatc aaaacttgca gcaggctttt tgacacttat gtcagttgcc
    11881 acattagctg cctgctcagg taaaacatca aatggaacaa atgttgttac aatgaagggc
    11941 gacactatca cagtctctga tttttatgat caagtaaaaa catcaaaagc tgcacaacaa
    12001 tcaatgctta cattgatcct ctcacgtgtt tttgatacac agtatggtga taaagtttca
    12061 gataaaaaag tatcagaagc ttataataag acagctaaag gctatggtaa ttcattttca
    12121 agcgcacttt cacaagcagg tttgactcca gaaggttaca aacaacaaat tcgcacaact
    12181 atgcttgtgg aatatgctgt aaaagaagca gctaagaaag aattaacaga agcaaactat
    12241 aaagaagcat ataaaaacta tactcctgaa acttctgtac aagtaatcaa attggatgca
    12301 gaagataaag ctaaatctgt ccttaaagat gtaaaggctg atggagctga ttttgcaaag
    12361 attgcaaaag aaaaaacaac agctactgat aaaaaagttg agtataaatt tgattctgca
    12421 gggacaagcc tccctaaaga agttatgtca gcagccttta agctagataa aaatggtgtt
    12481 tcagatgtgg tttcaacggt tgattcaaca acttataaaa caagttacta catcattaaa
    12541 gtaactgata agacagagaa aaaatctgat tggaaatctt acaaaaatcg cttaaaagaa
    12601 gttattctta aggataaaac aagcgataga tccttccaaa ataaagtgat ttcaaaagcc
    12661 ttagaaaaag ctaacgttaa gattaaagat aaagcatttg caggcatctt atcacaatat
    12721 gctacaacaa gtggttcatc atcacttaaa aaatagttga ccaagtctta aaaagactgt
    12781 ataattaata ctattgagaa tgaaatttca acgattagat acagaaaagt gacggttgct
    12841 gcgagtcatg gataatcaga aaaagatgct actcaatagg gtaaaactaa aaataaatga
    12901 gaatgacaag ttcattgaat gaaggtggta ccgcggtttt cgcccttcgt aatgggcttg
    12961 tcttttgcta tttgtcaact ttcgaggagg aagagatatg gctacttttc aaattaaaga
    13021 aaaaatgttt tctctaggcg gtaaatttac tattacagac cagactgggc ttccctgtta
    13081 tcacgttgaa ggaagtttgt tccctctacc taagactttt aaagtctttg acgaagaggg
    13141 gcatctgatt agtcagattg agaagaaggt tttaagtttt ttaccaaagt ttaatgtgac
    13201 attagccaat gggaatcatt tcacgattaa gaaagatttc tcatttttga aacctcatta
    13261 caccattgaa gaccttgata tggaagttaa gggaaatttt tgggatatgg atttccaact
    13321 cttaaaagac aatcaagtta tagcaaacat atctcaacag tggtttcgta tgacttcaac
    13381 ttatcaggta gaagtttaca atgaaactta caacgatttg acaatttcgc ttgtcatagc
    13441 gatcgactat gtcaaagagt tagaaaaaaa tgcatcaaac taattttagg agagaataat
    13501 gaaagaatta tcgtctgcac aaatccgcca aatgtggttg gatttctgga aatccaaagg
    13561 acattccgtt gagccttcag ctaacttggt tcctgttaat gacccaacgc ttctttggat
    13621 caactcaggt gttgcaacct tgaaaaaata ttttgatggt tcagtgattc cagaaaatcc
    13681 acgtattacc aatgcacaaa aatcaattcg tactaatgat attgaaaatg ttggtaaaac
    13741 agcacgtcac catactatgt ttgaaatgct tggtaacttc tcaattggag actatttccg
    13801 tgatgaagct attgagtggg gatttgaact cttgacaagt cctgaatggt ttgacttccc
    13861 taaagacaag ctctacatga cttattaccc agatgacaag gattcatata accgttggat
    13921 tgcttgtggc gttgaaccaa gtcacttggt gccaatcgag gataacttct gggaaatcgg
    13981 tgctggtcct tcaggtccag atactgagat tttcttcgac cgtggtgaag attttgatcc
    14041 agacaatatc ggacttcgtc ttttggctga agatatcgaa aacgatcgtt acatcgaaat
    14101 ctggaacatc gttctctcac aattcaatgc tgacccagcc gtaccacgtt cagaatacaa
    14161 agaattgcca aacaaaaaca ttgatacggg tgctggtctt gaacgtcttg cagctgttat
    14221 gcaaggggca aaaacaaact ttgaaactga ccttttcatg cctatcatcc gtgaagtaga
    14281 gaaattgtca ggtaaaactt acgatccaga tggcgacaac atgagtttca aggttatcgc
    14341 tgaccacatc cgtgcgcttt catttgctat cggtgatggt gcgcttcctg gaaatgaagg
    14401 tcgtggttac gttcttcgtc gtcttcttcg tcgtgcggtt atgcacggtc gccgtcttgg
    14461 catcaacgaa actttccttt acaaattggt tccgactgtt ggacaaatca tggaaagcta
    14521 ctacccagaa gtgcttgaaa aacgtgattt tatcgagaaa atcgttaaac gtgaggaaga
    14581 aacatttgct cgtactatcg atgcaggtag cggtcacttg gattcattgc ttgcgcagct
    14641 taaagctgaa ggtaaagata cacttgaagg taaagatatc ttcaaacttt atgatactta
    14701 tggattccca gtcgaattga cagaggaatt ggcagaagat gcaggctaca agattgacca
    14761 cgaaggcttt aagtcagcca tgaaagaaca acaagaccgt gcgcgtgcag ctgttgttaa
    14821 gggtggctca atgggtatgc aaaatgaaac tctagctggt atcgttgaag aatcacgctt
    14881 cgaatacgac acatatagtc ttgaatcaag tctttcagtt atcattgctg ataatgaacg
    14941 tactgaagct gtttcagaag gccaagccct tcttgtcttt gctcaaacac cattctatgc
    15001 tgaaatgggt ggacaggttg ctgacacagg tagaatcaaa aatgataagg gtgacacagt
    15061 tgctgaggtt gttgatgtcc aaaaagcacc aaatggtcaa cctctacaca ctgtaaacgt
    15121 tttggcatca ctttcagttg gaacaaacta cacacttgaa atcaacaaag agcgtcgttt
    15181 ggctgttgag aaaaaccaca cagcaactca cttgctccat gcagcccttc acaatgttat
    15241 cggtgaacac gcaactcagg ctggttcatt gaacgaagaa gaattcttgc gctttgattt
    15301 tactcacttt gaagcagtaa gcaatgagga acttcgtcac attgaacaag aagttaatga
    15361 gcaaatttgg aacgctctta caatcacaac gactgaaact gacgttgaaa ccgcaaaaga
    15421 gatgggagca atggcacttt ttggtgagaa atatggtaaa gtggttcgtg tggttcaaat
    15481 tggtaattat tctgttgaac tttgcggtgg aactcactta aataattctt cagaaatcgg
    15541 tctctttaag attgtcaaag aagaaggtat tggttcaggc actcgtcgta ttattgcagt
    15601 tactggtaga caagcttttg aagcttatcg taaccaagag gatgcactaa aagagatcgc
    15661 tgctactgta aaagctccgc aattgaaaga tgcagcagct aaagtacaag ctcttagcga
    15721 ctcacttcgt gatcttcaaa aagaaaatgt agagcttaaa gaaaaagcag cagctgcagc
    15781 agctggtgat gtttttaaag atattcaaga agctaagggc gtgcgcttca ttgctagtca
    15841 agttgatgtt gcagacgcag gtgcacttcg tacatttgct gataactgga aacaaaaaga
    15901 ctactctgat gtgcttgttc tcgtagcagc tattggtgag aaggttaatg tccttgttgc
    15961 aagcaaaact aaagatgtcc acgctggtaa catgatcaaa ggattggcac caattgtatc
    16021 aggtcgtggt ggtggtaaac cagacatggc tatggcaggt ggtagcgatg caagtaaaat
    16081 tgcagagttg ctagcagcag ttgcagaaaa tttataagaa acacctctca tttagttagt
    16141 aaataaaaca gacaaagggc agaacatcgt tctgttcctt tgtgtatata ggagaaatat
    16201 ggttggttta ggaacagtta tcaatgttat tcttattata gttggaggct ttgtaggttt
    16261 atttttgaaa aattttttga aagaatcact tcaaaagagc cttatgcaag ccatgggagt
    16321 tgcagtttta tttatttcga tatcaggtgt tttggagaaa atgatgttag tcgaaaagtc
    16381 acatctcatc tctaatcata caaatatgat gattattact cttgctctag gtacggtact
    16441 tggagagttg ctatcactag attcgtatat tgataaattt gggaactatc ttaagcaaaa
    16501 aacaggaagt ggtaacgata ttaaatttgt ggaggcattc gtcacctcaa cttgtaccgt
    16561 ttgtattgga gcaatggccg ttgttggtag cattcaagat ggtatagcag cagatcattc
    16621 gattttattt gctaagggaa tgttagacat gattattatt gcaattatga cagtgtcttt
    16681 agggaaagga gctctatttt ctgcacttcc tgtcgcttta ctacaaggta gccttacgat
    16741 tgttgctttt ttcatgggga gcctacttaa tccatcatct ttagattacc ttaacttagt
    16801 aggaaatatg cttatattct gtgtcggtgt taatttactc tttaacctaa atattaaagt
    16861 tatcaatatg ttgccagcaa ttattttagc tatattatgg ggaagtttta tataaaagat
    16921 aaccaagttt taactcaaac ttggtttttc tttagacata tgttaggatg aaagaagata
    16981 tactacggag aggatccaac tcatgaatct attattttct attgatgata tgtacgtaga
    17041 tcattttaaa gtgatgcttt attcgcttgt ccgacaaacc aagaatcgta agttagagat
    17101 atatgtctta caaaaaacat tactcaagcg tcatacagaa cttattcaat atactcaaaa
    17161 tttagaagtt ggctatcatc caataattgt aggtaccgaa gtatttgctc aggcacctac
    17221 aactgatcgc tatccagata caatatatta tcgacttttg gctcataaat ttttacctga
    17281 gaccttagat aggatacttt atttagatgc agatatgctt tgcttaaatg atttctcaag
    17341 tttatatgat atggagttag gtgatcaact atatgcagcg gcaagtcata atactgatgg
    17401 taagtttcta gattatgtca ataaattacg gttaaaaaat gtagagctag aatcctctta
    17461 tttcaataca ggggtactct tgatgaactt gcctgctata cgaaaagttg ttcatcaaca
    17521 aactatctta gattacatga tgcaaaaccg tggtcgtcta atcttaccag atcaggatat
    17581 cttaaatggc ttatacgcta atttggttaa accaatacct gatgaaattt ataattatga
    17641 tgctaggtat agtcttattt atcaattaaa aagtcgcaat gaatgggatt tagagtgggt
    17701 tattaaccat actgttttct tgcattttgc aggacgagat aaaccatgga aaaaagatta
    17761 tagaggacgt tactcgggcc tttataagtt tatggccaaa gaggctttaa ctaaaaattg
    17821 aagattcaaa aggttactaa atagaaataa tttagtaacc ttttttgggg tgatatttta
    17881 atgatatcaa taatcttt
//
LOCUS       DATRTN010000032        17805 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.032, whole genome shotgun sequence.
ACCESSION   DATRTN010000032 DATRTN010000000
VERSION     DATRTN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17805
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.032"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..239
                     /locus_tag="VBE22_001081"
     CDS             <1..239
                     /locus_tag="VBE22_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO7202549.1"
                     /translation="AINKSLRNVLHLLNIDSKMTAIGARHTYGSYLLAKGVDIWVVAR
                     LMGHKDITQLLETYGHVLTEVINKEYETVRSLVS"
     gene            complement(340..412)
                     /locus_tag="VBE22_001082"
     tRNA            complement(340..412)
                     /locus_tag="VBE22_001082"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(377..379),aa:Lys,seq:ctt)
     gene            complement(694..1962)
                     /locus_tag="VBE22_001083"
     CDS             complement(694..1962)
                     /locus_tag="VBE22_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEO7202550.1"
                     /translation="MADNLALRMRPRNINEVIGQQHLVGNGKIIDRMVAANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGNIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKKAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQRSYITMDKNGDGHYDILSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPEAQIHTVTALEAAQRIGFPEARILIANIVVDLALSPKSNSAYL
                     AMDAALADLRRSGNLPIPRHLRDGHYSGSKTLGNARDYKYPHAYPEKWVKQQYLPDKL
                     VGHNYFEANETGKYERALGSNKERIDKLSD"
     gene            complement(1955..2446)
                     /locus_tag="VBE22_001084"
     CDS             complement(1955..2446)
                     /locus_tag="VBE22_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001879696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7202551.1"
                     /translation="MIIRPMQDCDREAVLTLQNTGWTALTSPVYDRKWTESDLEKNLA
                     NGMSFFVAEVDDKIAGVLDFGPYYPFPAGKHVATFGILIAEPYQGQGLGKALLKALLT
                     EAKAQGYIKIAMHVMGNNSRAISLYQKYGFTEEARITKAFFIENHYVDALIFAKDLEN
                     NHG"
     gene            2773..3243
                     /locus_tag="VBE22_001085"
     CDS             2773..3243
                     /locus_tag="VBE22_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEO7202552.1"
                     /translation="MAKFGFLSVLEEELDKHLQYDFAMDWDKKNHTVEVTFILEAQNS
                     SAIETVDDQGETSSEDIVFEDYVLFYNPVKSRFDAEDYLVTIPYEPKKGLSREFLAYF
                     AETLNEVATEGLSDLMDFLTDDSIEEFGLSWDTDAFENGRAELKETEFYPYPRY"
     gene            3379..3837
                     /locus_tag="VBE22_001086"
     CDS             3379..3837
                     /locus_tag="VBE22_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7202553.1"
                     /translation="MDIFDELFDFSGAKIALFCEGKILTSLRDDFPDLPYAGFWDLPG
                     GGREDNETPLECLFREVDEELSLTLTRNHIDWVKTYRGMLKPDKLSVFMVGHISQKEY
                     DSIVLGDEGQDYKLMSIDEFLSHKKVIPQLQERLRDYLEVEDDYFTKGGS"
     gene            3809..4279
                     /locus_tag="VBE22_001087"
     CDS             3809..4279
                     /locus_tag="VBE22_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7202554.1"
                     /translation="MITLQKAEASDLEKIIAIQRASFKAVYEKYHDQYDPYVEEVEQI
                     RWKLVERPDCFYHFVLVDETIVGFLRLVIKDEEKRAWLGTAAILPQYQGQGYGSAVMA
                     LLEKTYPKLTKWDLCTIAQEKLMVSFYEKCGYHSTHTEPEQEGMDMVYMTKKIE"
     gene            complement(4318..5034)
                     /locus_tag="VBE22_001088"
     CDS             complement(4318..5034)
                     /locus_tag="VBE22_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7202555.1"
                     /translation="MKTVKEVSILSGVSVRTLHHYDKIGLFPPTALSEAGYRLYDDEA
                     LIRLQEILLFRELEFPLKDIKYLLEQAKEERQDLLAQQIKLLEWKRSHLEQVITHAKR
                     LQEKGDDYMNFDVYNKTELEQLQAEAKEKWGQTAAYKEFAQKHASDDFAQISQEMAKI
                     MVQFGQLKTQNVSDESVQMCVKRLQDYISQHFYTCTNEILAGLGQMYQSDDRFSQSID
                     KAGGAGTSEFVSQAIAYYCQ"
     gene            5158..5616
                     /locus_tag="VBE22_001089"
     CDS             5158..5616
                     /locus_tag="VBE22_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HEO7202556.1"
                     /translation="MEIVELLREWYSSFEVKEEKWNEKYEGILIRIEGDGNYKRCRLA
                     HKTASKMGYFTVFWQKDGNGKNIPFYEQDEVEDLIIVIKDGRWKGLFIIPKEVAVSKG
                     ILSSANSQGKMAMRFYPPWCSDLNRTALVTQRWQLNYFIDLSRNNEGVTT"
     gene            5613..6566
                     /locus_tag="VBE22_001090"
     CDS             5613..6566
                     /locus_tag="VBE22_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEO7202557.1"
                     /translation="MNTWNELTVHVNREAEEAVSNLLIETGSQGVAISDSADYLGQED
                     RFGELYPEVEQSDMIAITAYYPDTLDIEAVKADLADRLANFEGFGLATGSVNLDSQEL
                     VEEDWADNWKKYYEPARITHDLTIVPSWTDYEAKAGEKIIKMDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKDIYAYDLDDVAVRVAQENIDMNP
                     GTENIHVAAGDLLKGVQQEVDVIVANILADILIHLTDDAYRLVKDEGYLIMSGIISEK
                     WDMVRESAEKAGFFLETHMVQGEWNACVFKKTDDISGVIGG"
     gene            6566..7306
                     /locus_tag="VBE22_001091"
     CDS             6566..7306
                     /locus_tag="VBE22_001091"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEO7202558.1"
                     /translation="MQQYFVNGEAGAYVTIEDKDTIKHMFNVMRLTEDDQVVLVFDDA
                     IKRLAKVVDSSAHRFQILEELDNNIEMPVQVTIASGFPKGDKLDFVTQKATELGAAAI
                     WGFPADWSVVKWDGKKLAKKEDKLAKIALGAAEQSKRNRLPQVRLFEKKADFQAELAG
                     FDKIFIAYEESAKEGELSALAQNLQTVKAGDKLLFIFGPEGGISPKEIAAFEEVGAIK
                     VGLGPRIMRTETAPLYALSVISYSAELL"
     gene            7303..7890
                     /locus_tag="VBE22_001092"
     CDS             7303..7890
                     /locus_tag="VBE22_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO7202559.1"
                     /translation="MIIAIQGGMAVGKTTALKIIENVYPKIAISFEDISPVVDKINKH
                     QLDNNKLDDYCEIQQLFIQHERDRYELVRDNEIVLMDYGAEEIAFYTQYFPKVAGYSW
                     TLPSNLLLEVNRLKECFADYIIFLDADETVLYQRQKNDFNRQRHFFKWYTHHLLPAKK
                     HYLDTFSNVIHINTNHLNAEEVAEKIIDIIRKLTL"
     gene            8091..8972
                     /locus_tag="VBE22_001093"
     CDS             8091..8972
                     /locus_tag="VBE22_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEO7202560.1"
                     /translation="MKKHKMLSLLAVSGLMGIGILAGCSNDSSSSSKGTINIVSREEG
                     SGTRGAFIELFGIESKNKKGEKVDHTSDAATVTNSTSVMLTTVSKDPSAIGYSSLGSL
                     NSSVKVLKIDGKNATVKDIKSGSYKISRPFNIVTKEGKEKEATKDFIDYILSKDGQAV
                     VEKNGYIPLDNAKAYQAKVSSGKVVIAGSSSVTPVMEKIKEAYHKVNAKVDVEIQQSD
                     SSTGITSAIDGSADIGMASRELDKTESSKGVKATVIATDGIAVVVNKKNKVNDLSTKQ
                     VKDIFTGKTTSWSDLSK"
     gene            8987..9832
                     /gene="pstC"
                     /locus_tag="VBE22_001094"
     CDS             8987..9832
                     /gene="pstC"
                     /locus_tag="VBE22_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO7202561.1"
                     /translation="MKQTKEGLMQAVFFITACVSVISAILICLFLFSSGLPAITKIGW
                     GNFIFGKVWHPSNNIFGIFPMIVGSLYVTAGALLLGGPIGILTAVFMAYFCPENIYKP
                     LKSAINLMAGIPSVVYGFFGLVVIVPMIRQYIGGFGMGVLAASILLGIMILPTIVSIS
                     ESSLRAVPESYYEGGIALGASHERSVFFAVLPAAKRGILASVVLGIGRAIGETMAVIM
                     VAGNQAVLPQSLTSGVRTLTTNIVMEMGYSSGLHRQALIGTAVVLFIFILMINISFSA
                     LQKEE"
     gene            9834..10712
                     /gene="pstA"
                     /locus_tag="VBE22_001095"
     CDS             9834..10712
                     /gene="pstA"
                     /locus_tag="VBE22_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO7202562.1"
                     /translation="MENINQRTSKQYLASLKKDPLSFFLFAIVYLGAILSFATIAFVV
                     IYILVKGLPHVNTGLFAWTYNTQNVSLLPAFINTIFIIALTLLFAVPLGIGGSIYLTE
                     YARRDNPYLKIIRVATETLAGIPSIIYGLFGALFFVKYTHLGLSLISGSLTLSIMILP
                     LIMRTTEEALLSVPDSYREGAFALGAGKLRTIFKIVLPSAMSGIFAGIILAVGRIIGE
                     SAALIFTAGTVAKVAHSVFSSSRTLAVHMYAISGEGLYVDQTYATAVILLLLVIIVNF
                     VSGLVAKRLGSESNSE"
     gene            10705..11454
                     /gene="pstB"
                     /locus_tag="VBE22_001096"
     CDS             10705..11454
                     /gene="pstB"
                     /locus_tag="VBE22_001096"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO7202563.1"
                     /translation="MSKLVINNLDLYYGEFHALKDVNLDIEEKEITAFIGPSGCGKST
                     LLKSINRMNDLVKNCKITGDITLEGEDVYRQLDINQLRKKVGMVFQKPNPFPMSIYDN
                     VAFGPRTHGIHSKAELDDIVERSLKQAALWDEVKDRLHKSALGMSGGQQQRLCIARAL
                     AIEPDVLLMDEPTSALDPISTAKIEELVIQLKKNYTIVIVTHNMQQAVRISDKTAFFL
                     MGEVVEYNKTSQLFSLPQDERTENYITGRFG"
     gene            11451..12107
                     /gene="phoU"
                     /locus_tag="VBE22_001097"
     CDS             11451..12107
                     /gene="phoU"
                     /locus_tag="VBE22_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO7202564.1"
                     /translation="MIRSRFASQLNDLNKEIIFMGALCEDIIGKSLGALTNSNDVYLD
                     DISETYHKIEQMERDIEERCLKLLLRQQPVAKDLRRISSALKMVYDMKRIGAQAYEIA
                     EIVSLGHIIQGAGSERDQLNSMSNNVISMLTKSIDAFIYDNEEQAHQVIEQDRTVNQE
                     FDTIKKQLVLYFSVQDVDGEYPIDVLMIAKYLERIGDHTVNIAKWVLFSITGNLDGEA
                     "
     gene            12107..12784
                     /locus_tag="VBE22_001098"
     CDS             12107..12784
                     /locus_tag="VBE22_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7202565.1"
                     /translation="MIYCVEDDADIREMMLYTLQMAGFKAQGFSSSELFWEAIQEKVP
                     DLILLDIMLPGDDGLTILERLRRKHQTEMIPVIMTTAKGSEYDKVKGLDLGADDYLVK
                     PFGMMEMISRIKAVLRRSRQVDSKAHIIIGNLEIDPTNYWVKRGTEKIHLTLKEFELL
                     VLFFRNPNRVFTRQELLDKVWGEQFLGETRTVDVHIGTLRTKLGEDGYLIATVRGVGY
                     RLEERHD"
     gene            12777..14432
                     /locus_tag="VBE22_001099"
     CDS             12777..14432
                     /locus_tag="VBE22_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO7202566.1"
                     /translation="MTKKIFRTTLSASLGIVLVTILMIMGFLYNYFNHIQREQLRTQT
                     ALASQGISFEGKDYFENLKTSNVRITWVDNKGQVLYDTQSDAKHMKNHANRQEIKEAI
                     KSGYGESTRWSATLTEKSIYAAQRLNNGTIVRLSVAQQTIFYLLLGMISPLAIIILLA
                     IILSVLIARYIAKKVSEPLNNIDLDHPLSNDSYEEITPLLRRLDSHQAKIQHQKLLLQ
                     KRQKEFDTIISKIKEGMILLDDQARIVSINAEALKLFQINDDWHGRFMMEVSRDLTLK
                     DLIDQGLKGKKKEANIGIENNHYRVLVRPTTDNNRVTGLVVLLFDVTDQLQMEQLQRE
                     FTANVSHELKTPLHVISGYSELLANQMVPNEEVPQFAAKIHKESERLVKLVEDIINLS
                     HLDEQEKLPQETVNLYDLTQKVLEGLQAKADKKHIQINFNGEEAILRGNPVLLNSLVY
                     NLCDNAITYNHEKGQVNVTLKNSPDTITLEVSDMGLGIAEKDKKRIFERFYRVDKSRS
                     KIVGGTGLGLSIVKSALDFHNGSIKVDSHLGQGTTMTVLLHKQ"
     gene            14590..16773
                     /locus_tag="VBE22_001100"
     CDS             14590..16773
                     /locus_tag="VBE22_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO7202567.1"
                     /translation="MKNNVKQLFSFEFWQKFGKALMVVIAVMPAAGLMVSIGNSISLL
                     DPSNVLLGRIANVIAQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLSFILINLI
                     TGNFFGVKTDMLADSKATVQTVFGATIRVSDYFVNVLGQPALNMGVFVGIISGFVGAT
                     AFNKYYNYRKLPDALTFFNGKRFVPFVVIYRSVIVALILSVFWPVVQSGINGFGKWIA
                     SSQDSAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTQLGGTYTVLTGATKGAQVLGQ
                     DPLWLAWVGDLINLKGSNSSQYHHLLTSVTPARFKVGQMIGASGILMGLSYAMYRNVD
                     KDKKLKYKSMFISAAAATFLTGVTEPIEYMFMFAAMPLYLVYAVVQGCAFAMADIVNL
                     RVHSFGNIEFLTRVPMGIKAGLGGDIFNFVWVTLLFAVLMYFIANFMIKKFNLATAGR
                     NGNYDNEEVDNAPSTASGSADANSQVVQVINLLGGRDNIEDVDACMTRLRVTVKDGNS
                     VGSEAAWKKAGAMGLVLKGNGVQAIYGPKADVLKSDIQDLLDSGTVIPIVDLETGQPV
                     AAAPVTTYKGITEEIVSVANGQVEALDVVKDPVFSQKMMGDGFAVEPTDGNIYVPVSG
                     TVTSVFPTKHAFGLLTESGLEVLVHIGLDTVALDGQPFEVKISSGQKVVAGDLAVVAD
                     LEAIKAAGKETSVIIVFTNVSDIKTVKLEKSGPQIAKTVVAKVEL"
     gene            16812..17642
                     /locus_tag="VBE22_001101"
     CDS             16812..17642
                     /locus_tag="VBE22_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEO7202568.1"
                     /translation="MKGYSMAKCLTLNTHSWMEVNALKKLFDLAEHIFREKYDIICLQ
                     EVNQSISSPLAKSSPNYHPIEGTPALHQDNFALQLVHYLNLQGLHYHWTWAYNHIGYS
                     KYHEGVAILSLKPLKPEDILVSAVDDETDYHTRRALVAETTLNDKVVTVVSLHLSWFE
                     KGFAEEWKRLETTLLEVETPLLLMGDFNNPTGNQGYELVLNSPLALKDSHQIANHVFG
                     DHTIMADIDGWEGNKKALKVDHIFTSEDLSISSSQVVFEGGEAPVVSDHYGLEITMDF
                     "
BASE COUNT         5491 a         2937 c         3807 g         5570 t
ORIGIN      
        1 gtgccattaa taaaagtttg agaaatgtat tacatttatt aaatattgat tcaaaaatga
       61 ctgcaatagg tgcgagacat acctatggaa gttatttatt ggctaagggt gttgatattt
      121 gggtagttgc aagattaatg ggtcataagg atattacaca gttacttgaa acttatggtc
      181 acgtcttgac agaagtaata aataaggaat atgaaacggt cagaagttta gtatcttaaa
      241 gctaattttt aaagaatatc ttctagaaca aatttagaac aaatctgctg aaacaaagaa
      301 aaaaaccttt aaaatcaagg ttttcaacta tattattagt gccccctaca gggctcgaac
      361 ctgtgaccca tagattaaga gtctactgct ctaccaactg agctaaggag gctaggaagc
      421 tgtattggca ccgatgatcc actttttgta ttgatcccgc acaaaataag caggtgggta
      481 actcgcacct ctttaagtgg cttccgcgtg ttaacggctt gcgcactaga gatgacttcg
      541 tttcccgaaa tacaaagaaa tagtcggtca acacttagat gtggattcgt atgccacagc
      601 aatgtttcta tgtctttatg ataccacttt ttataaaatt ttcaagcaaa attatcgatt
      661 tttgaaatcg attgcgattt ttaaaactag caatcagtct gagagcttat caatgcgctc
      721 tttatttgaa ccaagtgcgc gttcgtattt accagtttca ttggcttcaa agtaattatg
      781 tcctactagt ttatctggta agtattgttg tttaacccat ttttcaggat aggcgtgagg
      841 gtacttgtaa tcccttgcat tgcccaaggt tttactaccg ctgtaatgtc catctcttag
      901 gtgacgtgga ataggtaaat ttcctgaacg acgtaaatca gctaaagctg catccatagc
      961 caaataggct gaatttgatt taggtgatag agctaaatcc acaacaatgt tagcaataag
     1021 aatacgagct tctggaaaac caatgcgttg ggcagcttcc aatgcagtca cagtatgtat
     1081 ttgtgcttcg gggttagcta agccgatatc ttcataagca ataatggtca aacgtcttgc
     1141 taaactaggt aaatctccgg cttctactaa acgagcggca tagtgcaggc tggcattgac
     1201 atcagaacct ctaattgatt tttgtaaggc tgataggata tcataatgcc catctccgtt
     1261 tttatccatt gtaatataac tacgctgcag actattttcc attgtctcta gactaatatg
     1321 acggctacca tcttcattag gagaagtaga catgactgct agatccaagg aattataagc
     1381 agatcgtagg tctccatttg tggcggtaac aataaaatct agcgcctcat catcaattgt
     1441 cactaaaaaa ggaaatcccc gttctttatc ggaaattgct agctgaatgg cttttttaat
     1501 atcctcgtta gaaagaggtt ctaactcaaa aatttgtacc ctacttctaa tggcaggagt
     1561 tactgaaaag aaaggatttt cagttgttgc tccaatcata ataatattgc cattctcaag
     1621 tagaggtaaa agaaaatctt gttttgtctt gtcaagtcta tgaatctcgt caagtaataa
     1681 aactaagcca ccagaaaatt tagcttcttc tgcaatttct tgaagacgtt ttttactatc
     1741 aacagtggca ttaaaagtac gaaaggcata tttagtagta ccagcaatag cagaagcaat
     1801 agaagtcttc ccaatacctg gtgggccata taggatcatg gaagataaca tattagctgc
     1861 aaccattcga tcaataattt tgccattacc aacgagatgt tgctgcccga taacttcatt
     1921 aatattgcga ggacgcatac gtagagcaag attgtcagcc atgattattc tccaaatctt
     1981 ttgcgaaaat taaagcatca acataatgat tttcaatgaa aaaggcttta gtgattcgag
     2041 cctcttcggt aaacccgtat ttttgataga gggaaatggc tctgctgtta ttgcccatga
     2101 catgcatggc aatcttgatg taaccttgag ctttagcctc agttagcaga gctttcaaga
     2161 gagctttacc cagtccctga ccttggtagg gttcagcgat gagaatgccg aaagtggcaa
     2221 catgcttgcc agctgggaaa ggatagtagg gaccaaagtc caagacgcca gcgatcttat
     2281 catcgacctc agcgacaaag aaggacatgc catttgccag attcttctcc aaatcactct
     2341 cagtccactt gcggtcatag acaggagagg taagagcagt ccagccagta ttttgtaagg
     2401 tcagaactgc ctcacggtcg cagtcctgca taggtctgat aatcataatt gatgatacaa
     2461 agggacggtt cggtttaccg aaaattttcg taaagaaata gggaacgacg aagtgtcgtc
     2521 agacattagg aagttcatct tttcttctag aaaattagtc ccgtattcaa atcctttcta
     2581 ttttctatat agagtttttc tcttggctga cgcttttaac atagtatgaa gtcagtcaaa
     2641 aacggctagt ctttgagact attgtaacat aaaaataagt aggaactacc ttttgattaa
     2701 cctgagtctg taacgttttt cttctatttc atgttataat aagaaagaat aaatagaaaa
     2761 taaggacata agatggctaa atttggattt ttatcggtac tagaagaaga attggataag
     2821 catttacagt atgactttgc catggattgg gataagaaaa atcatactgt agaagtcact
     2881 tttatattag aagcacaaaa cagctctgct attgaaaccg tggacgacca gggggagacg
     2941 agtagcgagg acatcgtttt tgaggactat gtactctttt acaaccctgt caagtctcgc
     3001 tttgatgctg aggactatct ggtgactatc ccctatgagc ctaagaaggg cttgtcgcgt
     3061 gagtttctgg cttattttgc ggagactctc aacgaggttg ccactgaagg gttgagcgac
     3121 ctcatggact tcctcaccga tgacagtatc gaagagttcg gtctttcttg ggatacggat
     3181 gcttttgaaa atggcagagc agaactgaaa gagacagagt tttaccctta tccgagatat
     3241 tagacttgat ggatttgcta aaatgtggat atacattgga tgatatagaa acagccagac
     3301 cagaggatat ggaacgttat tatgcccctg aacaaatcag aaaatatgga gctttaggaa
     3361 tagaattgag gttattgcat ggatattttt gatgagttat ttgatttctc aggtgcaaag
     3421 atcgctcttt tctgcgaggg aaagattttg accagcctaa gagatgattt tccagatttg
     3481 ccctatgcag gcttttggga cttaccaggc ggtggtcgtg aggacaatga aacacctttg
     3541 gaatgtcttt ttcgtgaagt tgatgaagaa ttaagtctga ccttaactcg aaaccatatt
     3601 gattgggtca agacttatag gggtatgttg aagcctgata aattatcagt ttttatggtt
     3661 ggacatatca gtcagaaaga gtacgatagc attgttctag gcgatgaagg acaagactac
     3721 aaactcatgt ccattgacga atttttaagc cataaaaagg tcattcctca gttgcaggag
     3781 aggttgagag attatttgga ggtggaagat gattacttta caaaaggcgg aagctagtga
     3841 tttagagaag attattgcca ttcagagagc tagctttaag gcagtctatg agaaatacca
     3901 cgatcaatac gatccctatg tggaagaagt tgagcagatt cgatggaaac tagtggagcg
     3961 accagattgt ttctatcatt ttgtccttgt tgatgagaca attgttggtt ttcttagatt
     4021 ggttatcaaa gacgaggaga agcgtgcttg gcttggtacg gcggctattc ttccccaata
     4081 tcaagggcag ggctacggct cagcagtgat ggctttatta gagaaaacct atcctaagct
     4141 tacaaagtgg gatttatgta cgattgcgca agaaaaacta atggtcagtt tttatgaaaa
     4201 atgtggttat cattcaaccc ataccgaacc tgagcaagaa ggaatggata tggtctatat
     4261 gacaaagaag atagaataaa aaaagaaaac tagatgattg tctagttttc tgatagttta
     4321 ttggcaatag taagcaatag cttggctgac aaattcactt gttcctgctc caccagcttt
     4381 gtcaattgat tgactaaacc ggtcatctga ttgatacatt tgtcctagtc cagcaaggat
     4441 ttcattggta caggtgtaaa aatgctgaga gatatagtct tgtagccgtt tcacacacat
     4501 ttgaacagat tcatcagata cattttgtgt ctttaattgc ccaaattgta ccattatttt
     4561 tgccatttct tggctaattt gtgcaaagtc atctgaagca tgtttttgag caaattcttt
     4621 gtaggctgct gtttgtcccc acttttcttt tgcttcagct tgtaattgtt ctaattctgt
     4681 tttattataa acatcgaaat tcatataatc atctcctttt tcttgtaatc gtttcgcatg
     4741 ggtgataacc tgctcaaggt gcgatctttt ccattcaagc aatttgattt gttgggctaa
     4801 taagtcttgc ctttcctctt ttgcttgctc aagcaggtat ttgatatctt tgagagggaa
     4861 ttccaactca cgaaatagca aaatttcttg caggcgaatc aatgcttcat cgtcataaag
     4921 tcgatagcct gcttcggata gggcggtagg tgggaataaa ccgattttat catagtgatg
     4981 caaggtgcgc acacttacac cgcttaagat actgacttct ttaactgttt tcatggtaat
     5041 aacctcctct cacttacata gtataaagga taacctaagg tcagggtcaa gcaaaaaata
     5101 aattattttg aaaagtatgg ttaatcatat tgatttgaag ataaaggagg gatagttata
     5161 gaaatagttg agctacttag ggaatggtat tcttcctttg aagttaaaga agaaaagtgg
     5221 aatgaaaaat atgaaggtat cctcataaga attgaaggcg atggaaacta taaaaggtgt
     5281 cgtttagctc ataagacagc tagcaagatg gggtatttta cagtattttg gcagaaagat
     5341 ggaaatggta aaaatattcc tttttatgaa caagatgaag tggaagattt gattattgtc
     5401 attaaagatg gtaggtggaa aggtttgttc atcattccca aagaggttgc agtgagtaag
     5461 ggtattcttt catctgctaa ctctcaagga aagatggcta tgcgatttta ccctccttgg
     5521 tgtagcgatt taaatagaac agctttggtg acacagcgat ggcagttgaa ctattttatt
     5581 gatttaagta gaaataatga aggagttact acatgaacac atggaatgaa ctaacggttc
     5641 acgtgaatcg tgaggcagag gaagctgtct cgaatttatt gattgaaact ggcagtcagg
     5701 gggttgccat tagcgattcg gctgactacc ttggacagga ggatcgtttc ggtgagcttt
     5761 atcctgaggt ggagcagtct gatatgattg ctattactgc ttattatcct gatacgcttg
     5821 atattgaggc ggttaaggct gatttggctg atcgtttagc taattttgaa ggctttggtc
     5881 ttgcgacggg tagcgtcaac cttgacagtc aggaattggt cgaagaggac tgggctgata
     5941 actggaagaa gtactatgag ccagctcgca ttacccatga tttgaccatc gtgccgtcat
     6001 ggacggacta cgaggctaag gctggtgaga agattatcaa gatggatcct ggtatggctt
     6061 ttgggactgg gactcaccca acgactaaga tgagtctttt tgccttggag caagtgcttc
     6121 gtggtggtga gacggttatc gatgtgggga ctggttctgg cgttttgtct attgctagct
     6181 ctctcttggg tgccaaggac atctatgcct atgatttgga tgatgttgca gtgcgcgtgg
     6241 ctcaggaaaa tatcgatatg aaccctggca ctgaaaatat tcatgtggct gctggtgatc
     6301 tgctcaaagg tgtccagcaa gaggtagatg ttattgttgc caatattttg gcggatattt
     6361 tgatccatct gacagacgac gcctaccgtt tggtcaagga tgagggctat ctcatcatgt
     6421 ctggtatcat ttctgagaag tgggacatgg tgcgtgagag tgctgaaaaa gctggtttct
     6481 tccttgagac tcacatggtt caaggtgagt ggaatgcctg cgttttcaaa aagacagatg
     6541 atatttcagg agtgattggc ggctaatgca acagtatttt gtaaatggcg aagcaggtgc
     6601 ctatgtgacc atcgaggaca aggataccat caagcacatg ttcaatgtca tgcgattgac
     6661 agaagatgac caagtggtct tggtttttga tgatgccatc aagcgtctgg caaaggtggt
     6721 ggacagctct gctcatcgct tccaaattct agaagagcta gacaacaata ttgaaatgcc
     6781 agttcaagtg accattgcct caggttttcc caagggagac aagttggatt ttgtgacgca
     6841 aaaggcaaca gaattgggtg ctgcggctat ctggggattc ccagctgact ggtcagtcgt
     6901 caagtgggac ggcaagaaac tagctaaaaa agaagataag ctggcaaaaa ttgctctggg
     6961 cgcagcggag cagagcaagc gtaatcgtct tcctcaggtt cgtttatttg agaagaaggc
     7021 tgattttcaa gcggagcttg caggctttga taagattttt atcgcctatg aagagtctgc
     7081 caaggagggt gaattgtcag cgctagccca gaacttgcag actgtaaaag caggtgacaa
     7141 gctcctcttc atctttggac cagaaggggg catttctcca aaagaaatcg cagcttttga
     7201 agaggttgga gctatcaagg tgggcttggg gccacgcatc atgcgcacag aaacagcgcc
     7261 gctctatgct ctaagtgtta ttagttactc agcggagtta ttatgattat tgctattcaa
     7321 ggtggtatgg ctgtagggaa aaccacagct ttgaaaatca ttgagaatgt ttaccccaaa
     7381 atcgctatta gttttgagga tatttctcca gtagttgata agattaataa acatcagctt
     7441 gataacaata aattagacga ttattgtgaa attcaacaac tatttattca gcatgagcga
     7501 gatagatacg aacttgttag agataatgaa atagtgttaa tggattatgg agctgaggaa
     7561 attgcttttt atacacaata ctttccaaag gtggcgggtt atagttggac tttaccttct
     7621 aatttgttac tagaggtaaa taggctaaag gaatgttttg ctgattatat tatttttcta
     7681 gatgcagatg agacggtcct ataccaacgt caaaaaaatg attttaatag acagcgtcat
     7741 tttttcaaat ggtatactca tcacctgctg cctgctaaaa aacattattt agacacattt
     7801 tccaatgtta ttcatattaa tacaaatcat ttaaatgctg aggaagtggc tgaaaaaatc
     7861 attgacatca ttcgtaagtt aactctataa tgggatagtt atactccaat tatgtgatgt
     7921 aacaactgtt tcaatccgac ttactggctg aattaccata atagaaaaaa ggagtttgga
     7981 taattttcca actcttttta attttacatt gattttacaa aaatcctcaa agagatttga
     8041 tatttcttaa ctataataaa atcaaatcaa aaaatattta ggagttcatc atgaaaaaac
     8101 acaaaatgct atctcttttg gcggtatctg gtcttatggg aattggcatc ctagcaggat
     8161 gctcaaatga ttcatcatct tcatctaaag ggacaattaa cattgtttct agagaggaag
     8221 ggtctggaac tcgtggagct ttcatagagt tatttgggat cgagtcaaaa aataaaaagg
     8281 gcgaaaaagt agaccatact tctgatgcag caactgttac caattcaact tcagttatgc
     8341 taaccactgt ctctaaagac ccgtcagcta ttggttattc ttcactaggt tcattaaata
     8401 gtagcgttaa agtgcttaaa attgatggca aaaatgcgac tgtaaaagat attaaatcag
     8461 gaagctataa gatttctcgt cctttcaata ttgtaacaaa agaaggtaag gaaaaagaag
     8521 caacaaaaga ttttattgat tatatcttaa gcaaagatgg gcaagcagtc gttgagaaaa
     8581 atggctatat tccacttgac aatgctaaag catatcaagc aaaagtgtca tctggtaaag
     8641 ttgttatagc aggttcaagt tcagtaacgc cggttatgga aaagattaaa gaagcctatc
     8701 ataaagtaaa tgcaaaagta gatgttgaaa ttcaacaaag tgattcttca actgggatta
     8761 caagcgcaat tgatggatca gcagatatag gtatggcatc acgtgaattg gataagacag
     8821 agtcatccaa aggggtcaaa gctactgtta ttgcaacaga tggtattgca gtagttgtta
     8881 ataagaaaaa taaagttaat gatctatcaa caaaacaagt aaaagacatt tttactggaa
     8941 agacaacgtc ttggtctgac ttatcaaagt aaggtaagag taaacaatga aacaaacaaa
     9001 agaaggtctt atgcaagctg tgtttttcat cacggcttgc gtcagtgtta tatcagctat
     9061 tttaatttgc ctctttctct tctctagtgg tcttccagct attacgaaaa tcggctgggg
     9121 aaattttata tttggtaaag tctggcatcc tagtaataat atttttggca tctttccaat
     9181 gattgtaggt tccttatatg ttacagcggg agctttactt ttaggaggac cgataggtat
     9241 ccttactgct gtttttatgg cttatttctg cccagaaaat atttataagc ctttaaagtc
     9301 agctatcaat ttaatggcgg gaatcccttc agttgtttat ggtttctttg gactcgttgt
     9361 tattgtccct atgataaggc aatatattgg tggctttggt atgggagtct tagcagcttc
     9421 aattttgcta ggaattatga ttttaccaac aattgttagc atttcagaat cttctttaag
     9481 agctgttcca gagagctatt atgaaggcgg tattgccctt ggtgcctctc atgaacgaag
     9541 tgtcttcttt gctgttttac cagctgctaa acgtggcatt ttagcatcgg ttgttttggg
     9601 aattggacgt gctattggtg aaacaatggc tgtcatcatg gtagcgggta atcaagctgt
     9661 cttgcctcaa tcgctgacat caggcgttag aactctaacc actaacattg ttatggaaat
     9721 gggctattct tctggtttgc atcgtcaagc cttaattggt acagctgtgg tactctttat
     9781 ctttatatta atgattaata tcagtttttc agcattacag aaagaggaat aagatggaaa
     9841 atattaacca aagaacgtca aaacaatatt tagcatcttt aaaaaaagat cccctttcct
     9901 tctttctttt tgctattgtc tatttgggag caattctaag ttttgctaca attgcatttg
     9961 ttgttattta tattttagtt aaggggcttc ctcatgtgaa tactggcttg tttgcatgga
    10021 cttataatac tcagaacgtt tcactattac cagctttcat taacactatc tttatcatcg
    10081 ccttaacttt attatttgca gttccacttg gtattggtgg atcaatttat ctaacggaat
    10141 atgctagacg tgataatcct tatctcaaaa ttatccgtgt tgcaaccgag acattggcag
    10201 ggataccttc aattatttat ggtttgtttg gtgcattatt ttttgtaaaa tacacacact
    10261 taggcttatc tttgatatcg ggttctctga ctttgagcat catgatttta cctttgatta
    10321 tgaggacaac tgaagaagct ttactatctg ttccggattc ttatcgagaa ggagcctttg
    10381 cgcttggtgc tggtaaattg agaaccatat ttaaaatcgt attacctagt gctatgtctg
    10441 gtatttttgc tggtattatt ttagcggtag gacgtatcat tggtgagtca gcagcactta
    10501 ttttcacagc tggaacagtt gcaaaagtag cacattcggt atttagttca tctcgtactt
    10561 tagcagtaca tatgtatgct atttcgggtg agggcttata tgttgaccaa acttatgcga
    10621 ctgcggtcat cttattgctt ttagttatta ttgtcaattt tgtatctgga ctagtagcca
    10681 aacgattagg aagtgagtcg aacagtgagt aaattagtaa ttaataattt agatttgtat
    10741 tatggagaat ttcacgctct gaaggatgtt aatttagaca ttgaagaaaa agaaatcaca
    10801 gcctttattg gaccttctgg gtgcggaaag tctactctct tgaaaagtat taaccgtatg
    10861 aatgatttgg tcaaaaattg caaaatcaca ggggatatta ctttagaagg cgaggatgtc
    10921 tatagacaat tggatattaa ccaattgcgt aaaaaggttg gaatggtgtt tcaaaaacct
    10981 aatccgtttc caatgagcat ttatgataat gttgcttttg gtccaagaac gcacggtatt
    11041 cattccaaag cagagttaga tgatatcgtt gaacgttccc ttaaacaggc ggctctatgg
    11101 gatgaggtta aagatcgtct tcacaaatct gctctgggta tgtctggcgg acagcagcaa
    11161 cgtttgtgta ttgcgcgtgc tcttgctatt gagcctgatg ttttacttat ggatgaaccc
    11221 acaagtgcct tggatcctat ttcaacagca aaaattgaag aattggttat ccaactgaag
    11281 aagaactaca ctattgttat agtgacacat aatatgcaac aagctgttcg tatttctgat
    11341 aaaacagcat tctttctgat gggagaagtg gttgaatata acaaaacgag ccaactcttt
    11401 tcattgccac aagatgagcg tacagaaaac tacattacag ggagattcgg atgattcgtt
    11461 cacgatttgc aagtcaactt aatgacctta ataaagaaat tatttttatg ggggcattgt
    11521 gtgaggacat cattgggaag tccttaggtg ctctaacaaa tagtaatgac gtttaccttg
    11581 acgatatttc tgaaacttat cacaagattg aacaaatgga acgggatatt gaggagagat
    11641 gtttaaaact tttactccgt cagcaaccag ttgccaagga tttacgccgt atttcttctg
    11701 cattaaaaat ggtttatgat atgaaacgta ttggtgctca agcttatgaa attgcggaaa
    11761 ttgttagttt aggtcatatt atccaaggtg caggttctga aagggatcaa cttaacagca
    11821 tgtctaataa tgtcattagt atgctgacta agagtataga tgcttttatt tatgacaatg
    11881 aagaacaagc gcatcaagta attgaacaag ataggacagt taaccaggaa tttgatacaa
    11941 taaagaaaca gttagtgtta tatttttcgg tacaggatgt tgatggtgaa tatcccatag
    12001 atgttttaat gatcgccaag tacctagagc gtattggtga tcatacagta aatattgcta
    12061 agtgggtgct tttctcaata acaggtaacc tagatggaga agcgtaatga tatattgtgt
    12121 tgaagatgat gctgatattc gcgaaatgat gctctatact ttgcaaatgg caggttttaa
    12181 agctcaagga ttttcgagtt cagagctttt ttgggaagct attcaggaga aagttccaga
    12241 cttgattttg cttgatatta tgttgccggg agatgatggt ttgactattt tagagcgttt
    12301 aaggagaaaa catcaaacgg aaatgatacc agtaatcatg acaactgcta aaggtagtga
    12361 gtatgataaa gttaaagggt tagatcttgg agcagatgat tatctcgtca aaccttttgg
    12421 gatgatggaa atgatttcac gaataaaagc agtcttgaga cgtagccgcc aagtagattc
    12481 aaaagctcat attatcattg gaaatttaga gattgatccg actaattatt gggtcaaaag
    12541 gggaacggaa aaaatccact taaccctaaa ggaatttgag ttattagtat tattcttccg
    12601 taatcccaat agagtcttta caaggcaaga acttctggat aaggtttggg gagaacaatt
    12661 tttaggagaa actagaactg tggatgttca cattgggaca ctgcgaacaa aacttggtga
    12721 ggatggctat ttgattgcta cagtaagggg agttggatac cgtttggagg aaagacatga
    12781 ctaaaaaaat tttccgtaca actttatctg ctagtttggg aattgtttta gttacaattt
    12841 tgatgattat gggatttctc tataattact tcaatcacat tcagcgagaa cagttgagaa
    12901 cacaaacggc actggcgtca caaggaattt cctttgaagg taaagattat tttgaaaatc
    12961 ttaaaacttc aaatgtgcgt attacttggg tagataataa gggacaagtg ctttatgata
    13021 cccaatcaga tgcaaaacat atgaaaaatc acgccaatcg tcaagaaatt aaagaagcta
    13081 tcaagagtgg ttatggtgag agcacgaggt ggtcagcgac acttactgag aagtctatct
    13141 atgctgcgca acgtttaaat aatggaacga tagttcgttt atcagttgcg caacaaacta
    13201 ttttttatct tctccttggt atgataagtc ccttagcaat tatcatctta cttgctatta
    13261 ttttatctgt attaattgca cgatatatag caaaaaaagt atcagagcct ttaaacaata
    13321 ttgacttaga tcatccttta tcaaatgata gttacgagga gataacacca ttattaaggc
    13381 gtcttgatag ccatcaggct aagattcaac atcaaaaact gttattacaa aagcgtcaaa
    13441 aagaatttga taccattatt tctaaaatta aggaaggaat gattctttta gatgatcaag
    13501 caagaatcgt tagtatcaac gcagaagctt taaagttatt ccaaattaat gatgattggc
    13561 atgggcgctt tatgatggaa gttagccgag atttaactct caaagactta atcgatcaag
    13621 gactaaaagg gaaaaagaaa gaggctaata ttggcattga aaataatcat taccgcgtct
    13681 tggtaagacc aactacagat aacaaccgag taacaggatt ggttgttcta ctgtttgatg
    13741 tgactgatca actccaaatg gaacaacttc aacgtgaatt tactgccaat gtctctcatg
    13801 aattgaaaac cccgcttcat gttatttctg gttactcaga actgttagct aatcaaatgg
    13861 ttccaaacga agaagtccca caatttgcag ctaaaattca taaagaatct gaacgattag
    13921 ttaaattggt tgaggatatt atcaaccttt ctcaccttga tgaacaagaa aaattaccgc
    13981 aagagacagt aaatctgtat gacttaacac aaaaagtttt agaaggttta caagctaaag
    14041 cagataagaa gcacatacaa atcaatttta atggagaaga agctattttg cgagggaatc
    14101 cagttttatt gaattccttg gtgtacaact tgtgtgacaa tgctatcacc tataaccatg
    14161 agaaaggtca agtaaatgtg actttgaaaa attcacctga tacaattacc ttagaagtta
    14221 gtgatatggg actaggcatt gcagagaagg acaaaaaacg tattttcgaa cgtttctatc
    14281 gcgtcgataa aagtcgctca aaaatagttg gggggactgg tttaggtttg tctattgtaa
    14341 agagtgcttt agattttcac aatggtagta taaaagtgga cagccatttg gggcagggaa
    14401 caacaatgac cgttttactt cacaaacaat aaaaactcac aaataaaagt ctagatgata
    14461 ttatctagac ttttttagta attcagaaaa aatatataag taagggttta caaaaaacaa
    14521 ataagtgcta taataaaacc ataataacgc aaacgattgc actattaagt gcataaaagg
    14581 agattgaaga tgaaaaataa tgtcaaacag ctctttagtt ttgaattttg gcagaagttt
    14641 gggaaggctt tgatggttgt tattgctgtt atgccagcag caggattgat ggttagtatt
    14701 ggtaactcca ttagtctatt ggatccatca aatgttttat tagggcgcat agctaatgtt
    14761 attgctcaaa ttggttgggg agttattggt aacttacaca tcttatttgc attggctatt
    14821 ggtggtagct gggctaaaga acgtgccggt ggtgcttttg cggctggctt atcctttatt
    14881 cttattaacc ttattacagg gaactttttt ggtgttaaaa cagatatgtt agcagacagt
    14941 aaagcaactg ttcaaacggt atttggtgca accatcagag tttcagatta ctttgttaac
    15001 gttcttgggc aacctgctct taatatgggt gtatttgtcg gtattatttc aggtttcgtt
    15061 ggtgcaactg cctttaataa atattataac tatcgtaaat taccagatgc tttaactttt
    15121 ttcaatggaa aacgttttgt tccatttgtt gttatttatc gttcagtcat tgttgcatta
    15181 attttatcag tattttggcc ggttgttcaa tcaggtatca atggctttgg taaatggatt
    15241 gcaagttcac aagacagtgc tccaatatta gcaccatttg tctacggtac gttagagcgt
    15301 ttattgctcc catttggttt acaccatatg ttaactattc caatgaacta cacgcaacta
    15361 ggtggcacat acactgtcct tacaggtgca actaaaggag cacaggtgtt gggacaagat
    15421 ccactgtggt tagcatgggt tggtgattta attaacttaa aaggctcaaa ttcaagtcaa
    15481 taccatcact tattgacaag tgtcactcca gcacgtttta aagtggggca aatgattggt
    15541 gcttcaggta ttttgatggg attatcttat gctatgtatc gtaacgttga taaagataaa
    15601 aaattgaaat acaagtcaat gtttatttca gcagcagcgg ctactttctt gacaggtgtt
    15661 actgagccaa ttgagtacat gttcatgttt gcagcaatgc cactctacct tgtatatgct
    15721 gtcgttcaag gatgtgcgtt tgctatggca gatattgtta acctccgcgt tcattcattt
    15781 ggtaatatag aatttttaac acgtgttcct atgggcataa aagcgggcct tggtggtgat
    15841 attttcaact ttgtttgggt gacacttctt tttgcagtat tgatgtactt tattgcaaac
    15901 tttatgatta aaaaattcaa tttagcaaca gcaggacgta atggtaatta tgataacgaa
    15961 gaggttgata atgctccttc aacagcttct ggttctgctg atgcaaattc tcaagttgta
    16021 caagttatta atttacttgg tggtcgtgat aacattgaag atgtagatgc atgtatgact
    16081 cgtcttcgtg tcacagttaa agatggaaat agcgtgggtt ctgaagcagc ttggaaaaaa
    16141 gctggtgcaa tgggattggt gttaaaaggt aatggcgttc aagctattta cggacctaaa
    16201 gcagatgtcc ttaaatcaga cattcaagat ttgctagatt caggtacagt tattccaata
    16261 gttgatttag aaacaggtca acctgtagca gcagccccag ttacaactta taaaggaata
    16321 acagaagaaa ttgtttcggt ggctaatggt caagtagaag cattggatgt tgttaaagac
    16381 cctgtctttt ctcaaaaaat gatgggtgat ggctttgctg tggaaccaac tgatggcaat
    16441 atttatgttc cagtttcagg tacagttact agtgtctttc caacaaaaca tgcttttggt
    16501 ttattaacag aaagtggcct agaagtttta gtacatatcg gtctagatac agtagctctt
    16561 gatggtcaac catttgaggt taaaatttct agtggtcaaa aagtggtagc aggcgatctt
    16621 gctgtagtag cagatttgga agctatcaaa gcagctggta aagagacaag tgtgattatc
    16681 gtctttacaa atgtttcaga tataaaaact gttaaacttg aaaaatcagg tccacaaata
    16741 gctaaaacag ttgtggcaaa agtcgaactg taacaaacga taggagctgg gcaagtctca
    16801 gcttctttct gatgaaagga tactctatgg caaaatgctt aacgttaaat actcattcat
    16861 ggatggaagt aaatgctcta aagaaattat ttgatttagc agagcatatc ttccgtgaaa
    16921 aatatgatat catctgccta caagaggtga accagagcat atcttcccct cttgctaaga
    16981 gtagtccaaa ttatcatccg attgaaggta caccagctct tcatcaagat aactttgcct
    17041 tgcaattagt gcattaccta aatcttcaag gtctacacta tcattggaca tgggcttata
    17101 atcatattgg ttatagtaag taccatgaag gggtagcgat actttctctg aaaccattaa
    17161 agccagagga tattttagtt tcagctgtag acgatgagac tgattatcat actcgtcgtg
    17221 ctctggtagc tgagaccact ctgaatgaca aagtcgttac agtagttagc ctccatttat
    17281 catggtttga gaaaggattt gcggaggagt ggaaacgact agaaactacc ctacttgagg
    17341 tagaaactcc acttttactc atgggtgact ttaataatcc tactggaaat cagggttatg
    17401 aacttgttct taatagtcca ttagcattaa aggatagcca ccaaatagcc aatcatgtat
    17461 ttggggacca tactattatg gctgatattg atggttggga agggaataaa aaggctctta
    17521 aagttgacca tatttttact agcgaagacc tttcaatcag ttcaagtcaa gtagtttttg
    17581 aaggtggtga agcacctgtc gttagtgatc attacggctt ggaaattacc atggatttct
    17641 aatatcttat aataaaagat acccttaggt tcagataaag tctgtatcta agggtatttt
    17701 aatagaaata atttattttt aatacttaac tacttaatta ggtgttcaaa tagttatata
    17761 tatgttaaaa ttagcatata tttattatgc ttttaaaatt agcct
//
LOCUS       DATRTN010000033        17728 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.033, whole genome shotgun sequence.
ACCESSION   DATRTN010000033 DATRTN010000000
VERSION     DATRTN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17728
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.033"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..116)
                     /locus_tag="VBE22_001102"
     CDS             complement(<1..116)
                     /locus_tag="VBE22_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202569.1"
                     /translation="MLLQSPISNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFR"
     gene            complement(196..1734)
                     /locus_tag="VBE22_001103"
     CDS             complement(196..1734)
                     /locus_tag="VBE22_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7202570.1"
                     /translation="MSKQKVMATLLLSTLVLSLSSPLVTLAETINPETSLTMATASTE
                     SSSEAEKQEKTQPTDSETASPSAEGSISTEKTEIGTTETSSSNESSSSSSHQSSSNED
                     AKTSDSASTASTPSTNTTNSSQADSKPGQSTKTELKPEPTLPLVEPKITPAPSQIESV
                     QTNQNASVPALSFDDNLLSTPISPVTATPFYVEHWSGQDAYSHYLLSHRYGIKAEQLD
                     GYLKSLGIQYDSNRINGAKLLQWEKDSGLDVRAIVAIAVLESSLGTQGVAKMPGANMF
                     GYGAFDHDSSHASAYNDEEAIMLLTKNTIIKNNNSSFEIQDLKAQKLSSGQLNTVTEG
                     GVYYTDNSGTGKRRAQIMEDLDRWIDQHGGTPEIPAALKALSTASLADLPSGFSLSTA
                     VNTASYIASTYPWGECTWYVFNRAKELGYTFDPFMGNGGDWQHKAGFETTHSPKVGYA
                     VSFSPGQAGADGTYGHVAIVEEVKKDGSVLISESNAMGRGIVSYRTFSSAQAAQLTYV
                     IGHK"
     gene            complement(1827..2927)
                     /locus_tag="VBE22_001104"
     CDS             complement(1827..2927)
                     /locus_tag="VBE22_001104"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO7202571.1"
                     /translation="MISSYHRPTRALIDLEAIANNVKSVQEHIPSDKKTFAVVKANAY
                     GHGAVEVSKYIESIVDGFCVSNLDEAIELRQAGIVKMILVLGVVMPEQVILAKNENIT
                     LTVASLEWLRLCQTSAVDLSGLEVHIKVDSGMGRIGVRQLDEGNKLISELGESGASVK
                     GIFTHFATADEADNCKFNQQLTFFKDFISGLDNCPDLVHASNSATSLWHSETIFNAVR
                     LGVVMYGLNPSGTDLDLPYPINPALSLESELVHVKQLHDGSQVGYGATYQVTGDEFVG
                     TVPIGYADGWTRDMQGFSVIVNGELCEIIGRVSMDQMTIRLPQKYTIGTKVTLIGQQG
                     SCNITTTDVAQKRQTINYEVLCLLSDRIPRYY"
     gene            complement(2924..3283)
                     /locus_tag="VBE22_001105"
     CDS             complement(2924..3283)
                     /locus_tag="VBE22_001105"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEO7202572.1"
                     /translation="MIVGHGIDLQEIEAITKAYERNQRFAERVLTEQELLLFKGISNP
                     KRQMSFLTGRWAAKEAYSKALGTGIGKVNFHDIEILSDDKGAPLITKEPFNGKSFVSI
                     SHSGNYAQASVILEEEK"
     gene            complement(3308..4315)
                     /locus_tag="VBE22_001106"
     CDS             complement(3308..4315)
                     /locus_tag="VBE22_001106"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO7202573.1"
                     /translation="MGFHQLSDKINIEILKQKTSLDLEVSQKKLAKEEELKNIIKGED
                     QRFLVIVGPCSADNPKAVLTYAKRLAKLEAAFKDKMFLVMRVYTAKPRTNGDGYKGLV
                     HHSDKLGVFFQARKMHYDIIRETGLLTADELLYPEMLSVMDDLVSYYAIGARSVEDQG
                     HRFISSGIDAPVGMKNPTSGNLRVMLNAVYAAQNQQELFYQNKQVRTDGNLLSHVILR
                     GYHNADYRSIPNYHYENLLETITHYEETDLQNPFIVVDTNHDNSGKQFLEQIRIVKSV
                     LADRQWHTKIRNYVRGFLIESYLEDGRQDKPDVFGKSITDPCLGWDKTEMLIRDIYNS
                     L"
     gene            complement(4441..6969)
                     /gene="secA"
                     /locus_tag="VBE22_001107"
     CDS             complement(4441..6969)
                     /gene="secA"
                     /locus_tag="VBE22_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEO7202574.1"
                     /translation="MANILRTVIENDKGELKKLDKIAKKVDSYADHMAALSDEALQAK
                     TPEFKERYQNGETLDQLLPEAFAVVREASKRVLGLYPYHVQIMGGIVLHHGDIPEMRT
                     GEGKTLTATMPVYLNAISGLGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PFEKREAYNCDITYSTNAEVGFDYLRDNMVVRQEDMVQRPLNYALVDEVDSVLIDEAR
                     TPLIVSGPVSSEMNQLYTRADMFVKTLNSDDYIIDVPTKTIGLSDTGIDKAENYFHLN
                     NLYDLENVALTHYIDNALRANYIMLLNIDYVVSEEQEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKESVPIQEESKTSASITYQNMFRMYHKLAGMTGTGKTEEEEFREIYNMRVIP
                     IPTNRPVQRIDHSDLLYPTLDSKFRAVVADVKERYEQGQPVLVGTVAVETSDLISRKL
                     VAAGVPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGTDRIKVVLERMNLAE
                     DDTVIKSKMLTRQVESAQRRVEGNNYDTRKQVLQYDDVMREQREIIYANRREVITAER
                     DLGPELKGMIKRTIKRAVDAHSRSDKNTAAEAIVNFARSALLDEEAITVSELRGLKEA
                     EIKELLYERALAVYEQQIAKLKDPEAIIEFQKVLILMVVDNQWTEHIDALDQLRNSVG
                     LRGYAQNNPIVEYQSEGFRMFQDMIGSIEFDVTRTLMKAQIHEQERERASQHATTTAE
                     QNISAQHVPMNNESPEYQGIKRNDKCPCGSGMKFKNCHGLRCLQ"
     gene            complement(7078..8025)
                     /gene="manA"
                     /locus_tag="VBE22_001108"
     CDS             complement(7078..8025)
                     /gene="manA"
                     /locus_tag="VBE22_001108"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEO7202575.1"
                     /translation="MSEPLFLEASMHDKIWGGTKLRDEFGYDIPSETTGEYWAISAHP
                     NGVSRVKNGRFKGCFLDKLYQGEKSLFGNPDDTVFPLLTKILDANDWLSVQVHPDDAY
                     ALKHEGELGKTECWYIISADEGSEIIYGHNAKTKEELRQMIESGDWEHLLTRIPVKSG
                     DFYYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRPDASGKLRDLHIEQSIDVLTI
                     GKPANTVPANMKLKHLSSTLLVSNDFFTVYKWEISGVTNFKQFAPYLLVSVLDGAGHI
                     TVDNKVYTLKKGDHFILPNDVVKWDIDGQLEIIASHVKK"
     gene            complement(8143..9024)
                     /locus_tag="VBE22_001109"
     CDS             complement(8143..9024)
                     /locus_tag="VBE22_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO7202576.1"
                     /translation="MTKLYGSIEAGGTKFVCAVGDEELKVVEKIQFPTTTPQETIKKT
                     VDFFKRFEKKLEAVAIGSFGPIDIDKKSKTYGYITTTPKLHWANVNLLGLISKDFNVP
                     FYFTTDVNSSAYGEVIARNNIDSLVYYTIGTGIGAGAIQKGEFIGGTGHTEAGHTYMA
                     MHPQDQANDFKGICPFHNSCLEGLASGPTLEARTGIRGELIEENSMVWDVQAYYIAQA
                     AIQATVLYRPQVIVFGGGVMAQKHMLRRVRQTFATLLNGYLPVPNLSDYIVTPAVEEN
                     GSATLGNFALAKKISKG"
     gene            complement(9092..11011)
                     /locus_tag="VBE22_001110"
     CDS             complement(9092..11011)
                     /locus_tag="VBE22_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO7202577.1"
                     /translation="MASNTEIAKQVINAIGGASNVRSVAHCATRLRVMVKDETVIDKN
                     TVENIEKVQGAFFNSGQYQIIFGTGTVNKIYDEVVAQGLPTSSTSDQKAEAAKQGNAF
                     QRAIRTFGDVFVPLLPAIVATGLFMGIRGAINNDTVLALFGTTSKAFSSSNFYTYTVV
                     LTDTAFAFFPALISWSAFRVFGGNPVIGLVLGLMMVNSALPNAWAVASGDAHPIKFFG
                     FIPVVGYQNSVLPAFFVGLLGAKLEKWLHKKIPDVLDLLLVPFLTFTVMSILALFVIG
                     PIFHSVENYVLAGTKFVLNLPLGLSGLILGGVHQIIVVTGVHHIFNLLEAQLIAADGK
                     DPFNAIITAAMTAQAGATLAVGVKTKNKKLKALAFPAALSAGLGITEPAIFGVNLRFG
                     KPFIMGLIAGAAGGWLASILKLAGTGFGITIIPGTLLYLNGQIVKYLIMVIGTTALAF
                     VLTYMFGYEDKDEKVVAEVSPLVEETDDNPTITQTSQLRAETIVSPLDGQVIALDTVS
                     DPVFSSGIMGDGLAIKPSGNTIYSPVDGFVQIVFETGHAYGIKSDKGAEILIHIGIDT
                     VTMNGTGFTSKVKADQKVKKGDILGTFDSAKIAEAGLDNTAMIIVTNTADFADVIPLV
                     KEGVVTTGDMIIDVK"
     gene            11196..12635
                     /locus_tag="VBE22_001111"
     CDS             11196..12635
                     /locus_tag="VBE22_001111"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEO7202578.1"
                     /translation="MNLPTEIRYRPYDEWTEEDKENIVKNVSKSPWRATYHLEAKTGL
                     LNDPNGFSYFNGKFHLFYQNWPFGAAHGLKQWVHTESDDLVHFKETGIKLKPDHVNDS
                     HGAYSGSALAIDDKLFLFYTGNVRDMKWNRDPRQIGAWMTSDGKITKFDKVLISQPND
                     VTEHFRDPQIFNYDNQFYAVIGAQNSKKCGFIKLYKALNNDIHHWEFVGDLDFGGTGS
                     EYMIECPNIIFVKGKPVLLYSPQGLDKNELDYQNIYPNTYKIGQYFDANSSKIVEPSP
                     IYNLDYGFEAYATQGFNTSDGRAFIVSWIGLPDIDYPSDQFDYQGAMSLVKELSIKNG
                     NLYQYPVPAMKNLRQHQAEFKTQLQTNNTYELELLVPRNDLSSFVLFANPKGQGLSIT
                     IDTAKGKVIVDRSQAGQQYATEFGTSRQCNIPKDATSVNVFIDKSIFEIFINKGEKVF
                     TGRVFPDAEQSGIQLKEGHVHGKYFELKY"
     gene            12637..13599
                     /locus_tag="VBE22_001112"
     CDS             12637..13599
                     /locus_tag="VBE22_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7202579.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVTKVNEAMRTL
                     GYKPNNLARSLQGKSAKLIGLIFPNIRNIFYAELIEHLEIELFKHGYKTILCNSEKDP
                     IKEKEYLEMLGANQVDGIISSSHNLGIDDYEKVEAPIVAFDRNLAPHIPIVSSDNFFG
                     GKMAAQTLKKHGCQKMIMITGNDNSDSPTGLRRLGFSYESKESKVITVTNGLSNMRRE
                     MELKSIISTHKPDGIFTSDDLTALLVIKLISQLGLSIPEDIKVIGYDGTSFIQDYVPH
                     LTTIKQPIREIAQLMVEILLAKIEGQKTNKDYILPVSLIPGSSV"
     gene            complement(13686..14120)
                     /gene="nusB"
                     /locus_tag="VBE22_001113"
     CDS             complement(13686..14120)
                     /gene="nusB"
                     /locus_tag="VBE22_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEO7202580.1"
                     /translation="MTSVFKDSRRDLRERAFQTLFSLETGGEFIDAAHFAYGYDKTVS
                     EDKVLEVPIFLLNLVNGVVDHKDELDTLISSHLKSGWSLERLTLVDKSLLRLGLYEIK
                     YFDETPDRVALNEIIEIAKKYSDETSAKFVNGLLSQFITNEN"
     gene            complement(14113..14502)
                     /locus_tag="VBE22_001114"
     CDS             complement(14113..14502)
                     /locus_tag="VBE22_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7202581.1"
                     /translation="MTTENLGEIVISPRVLEVITGIAATKVDGVHSLRNKAVTDSLSK
                     KSLGRGVYLKNEEDDTVAADIYVYLQYGVNVPAVSIAIQQAVKTAVYDMAEVKISSVN
                     IHVEGIVPEKTPKPDLKSLFDEDFLDD"
     gene            complement(14591..15151)
                     /gene="efp"
                     /locus_tag="VBE22_001115"
     CDS             complement(14591..15151)
                     /gene="efp"
                     /locus_tag="VBE22_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEO7202582.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNNETYDQYEIPTVNIENE
                     LLYILENSEVKIQFYGTEVIGVQIPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(16330..>17728)
                     /locus_tag="VBE22_001116"
     CDS             complement(16330..>17728)
                     /locus_tag="VBE22_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202583.1"
                     /translation="THDLTDLSQTVMADVSKIEQALSHAICAYYGWIGYFVFLSLSLL
                     IGSLQLGLCIIVPVITALLILYLSKNIQYRINRKYYKKLRENSQAFQDSFEMQQDIKS
                     YNMQHTVRKDVQDKLKDSEKIQIKTELFTTIPSSLLSILPQFSIALVIIFGFQLYLTG
                     QIQLIFYIGYIICATKVASGFTGMLDSLLMIINFQSSFKQVTAIRKTPLQEGVNADIQ
                     TFNVEFRDVSFAYEKGRNVVSNLSFNANQGEVTAIVGPSGCGKSTILKLLSRLYDYNA
                     GQILIGGKDISEISTNSLFENISTVFQDVELFDDTIFENIKCGNPKATIEEVLEAAKI
                     ANVDEIADKFVDKYDTKIGENGSKLSGGERQRISIARAILKDAPIILLDEISASLDIE
                     NEIKIQAGLDKLIKNKTVIIVSHRMKSIENANKIIVIENGKVENFGTHSYLLENSTRY
                     RKLVECSQLASEYEY"
BASE COUNT         5584 a         3465 c         2790 g         5889 t
ORIGIN      
        1 cgaaacggat aataaagaag taaatcttct actgtataaa tatctaattt ctgaaatttt
       61 tctgctgatt ttggtccaaa tcccttaaga ttagaaattg gtgattgtaa tagcataaga
      121 ttctccttct aatatctttt attatactaa aagatcacca aaaaggcgcc tcaagagcgc
      181 ctttattatg tctaattatt tatggccaat aacataagtt aattgtgcag cttgtgctga
      241 actaaaagta cggtaagaga caataccacg tcccattgca ttagattctg aaataagaac
      301 tgaaccatct tttttaactt cttcaacaat agctacgtga ccgtaagtgc catcagcacc
      361 agcttgtcct ggtgaaaatg atacagcata gcctactttt ggtgaatgtg ttgtttcaaa
      421 accagcctta tgttgccaat ctccaccatt acccataaat ggatcaaatg tataacctaa
      481 ctctttagcg cggttaaaga cataccatgt acattcaccc catggataag ttgatgcaat
      541 atagctagct gtgttaactg ctgttgataa gctaaaacca cttggtaaat ctgctaaact
      601 tgctgtcgat aaagctttca aggcagcagg aatttctggt gtccctccat gttgatcaat
      661 ccagcggtct aaatcttcca taatctgggc acgacgttta ccagttccag agttatctgt
      721 ataataaaca ccaccctcag taactgtatt aagttgtcca gaagataatt tctgtgcttt
      781 caaatcttgg atttcaaagc tagagttgtt atttttaata attgtatttt ttgtcaacaa
      841 cataattgct tcttcatcat tataagcact agcatggcta gagtcatgat caaaggcacc
      901 ataaccaaac atattagcac ctggcatctt agccactcct tgagttccca atgaactttc
      961 aaggacagca atagctacaa tagcacggac atctaaacca ctatcttttt cccattgtaa
     1021 taacttagca ccattgatac gattagaatc atattgaatc cctaaagatt ttaagtaccc
     1081 atctaattgt tcagctttga taccgtaacg atgtgacaat aaatagtgag agtaggcatc
     1141 ctgaccagac cagtgttcta cgtagaatgg cgttgctgtc actggtgaaa tcggtgttga
     1201 taataaatta tcatcaaagg ataaagcagg aacagaagca ttctgatttg tctgaacact
     1261 ttctatctga gacggagcgg gagttatttt aggctctact aatggtaagg taggctcagg
     1321 ttttaattca gtctttgttg attgacctgg cttactgtct gcttgactac tgtttgtagt
     1381 attagtgcta ggagtagatg ctgttgaagc agaatcagat gttttagcat cttcgttgga
     1441 agaagattga tgtgatgaac ttgatgatga ttcattgctt gatgatgtct ctgtcgtacc
     1501 aatctctgtt ttttctgttg agatacttcc ttcggctgaa ggtgaagcag tttctgaatc
     1561 tgtaggttgt gttttttcct gtttctctgc ttcagaagaa ctttctgttg atgctgttgc
     1621 cattgtcagg cttgtttctg gattaatagt ttctgctaag gtcactaaag gtgatgatag
     1681 cgataagact aaagtggata acaacaaagt tgccattact ttttgttttg acatatacta
     1741 atgatttcct tctataaaaa ttgtcactga ttagtttaac atatttttaa attttttaaa
     1801 acagttaact actgtgtcat ttgtttttaa taatatctcg gaatacgatc gcttaaaaga
     1861 caaagtactt cataattaat cgtctgtctt ttttgagcga cgtccgtagt tgtaatgtta
     1921 caactacctt gttgaccaat caaggttact ttagttccga tagtatattt ttgaggtaaa
     1981 cggatagtca tttgatccat cgagacacgc ccaataattt cacaaagttc tccatttact
     2041 ataactgaaa atccttgcat atctcgtgtc catccatcag cgtagccaat cggtactgtt
     2101 ccaacaaatt cgtctccagt aacttgatag gttgcgccat agccaacttg agaaccatcg
     2161 tgtagttgtt taacatgtac tagctcagac tctaaactta aagcagggtt tatagggtaa
     2221 ggtaagtcta aatcagttcc acttggattt aagccgtaca taacaacacc caatctaacg
     2281 gcattaaaaa ttgtctcact atgccaaagg cttgtagcag aattactagc atgaacaagg
     2341 tccggacaat tatctaatcc tgaaataaag tctttaaaaa aagtaagttg ttgattgaat
     2401 ttacagttat cggcttcatc agctgttgca aaatgggtga aaatcccctt gacagaggca
     2461 ccactttccc ccaattcaga aattagctta tttccttcat ctaactggcg aacacctata
     2521 cgtcccattc cactatcaac tttgatatgt acttctagac cagataaatc tactgctgat
     2581 gtctgacata aacgtaacca ctccaagcta gcaactgtta gagtaatatt ttcatttttg
     2641 gctagtataa cctgctcagg cataacaaca cctaatacca atatcatttt tacaattcct
     2701 gcttgacgta gctcaatagc ctcatctaaa ttggatacgc aaaaaccatc tacaatagac
     2761 tcaatgtatt ttgaaacttc aacagcacca tgcccataag cattagcctt aactacagca
     2821 aatgtttttt tatcacttgg aatgtgctct tgtacagatt taacgttatt tgcaatcgcc
     2881 tctaaatcta tcaaggctct ggttggtcta tgataacttg aaatcatttt tcttcctcca
     2941 aaataacact agcttgtgca taattaccac tatgagatat tgaaacaaaa gattttccat
     3001 taaacggttc ttttgtaatc aaaggcgctc ctttatcatc cgataaaatt tcgatatcat
     3061 gaaaattaac tttcccaatt cctgttccaa gtgctttgct ataagcctct tttgctgccc
     3121 atcgccctgt taaaaaagac atctgacgct tgggattgga aattccttta aaaagaagca
     3181 attcttgttc ggtcaaaacg cgttctgcaa aacgttgatt acgctcatat gctttagtaa
     3241 tcgcctctat ctcttgtaaa tcaattccgt gtccaacaat catgtgtaac tcctataagt
     3301 tcgatactta caaactattg taaatatccc taattagcat ttctgtttta tcccaaccta
     3361 gacatggatc tgtgatagac ttaccaaaaa catctggttt atcttgtctt ccatcttcta
     3421 aataagattc tatcaaaaaa ccacgaacat agtttctaat tttagtatgc cactgtcggt
     3481 ctgcaagtac tgatttcaca atgcgaattt gttctaaaaa ttgcttacct gaattgtcat
     3541 gatttgtatc aacaacaata aagggatttt gcaaatcagt ctcttcataa tgagtaatag
     3601 tttctaaaag gttttcataa tgataattag ggatacttcg atagtcggca ttatgatatc
     3661 ctctcaaaat aacatgtgac agcaaattac catctgttct aacttgctta ttttgataga
     3721 ataattcctg ttgattttgt gctgcataga ccgcattaag catgactctt aaattaccag
     3781 acgttggatt tttcatacct actggtgcat ctatacctga agatataaaa cggtgtccct
     3841 gatcttccac tgaacgcgca ccaatagcat agtaagaaac taaatcatcc atcactgata
     3901 acatctctgg ataaagtaat tcatcagctg tcaggagtcc agtctctcga atgatgtcat
     3961 agtgcatctt acgtgcttga aaaaaaactc ctagtttgtc agaatggtga actaagcctt
     4021 tataaccatc accattcgtt cttggcttag cggtataaac tctcataacc aaaaacattt
     4081 tatctttaaa ggcagcttcc aatttagcta aacgctttgc ataagttagc accgcctttg
     4141 gattatccgc agaacaagga cctactatta ctagaaaccg ctgatcctca ccttttatga
     4201 tattttttaa ctcttcttct ttggctaatt ttttttggct aacttctaga tctaagctag
     4261 ttttctgctt caatatttca atattaattt tatcacttaa ctgatgaaat cccatgtttt
     4321 ttcctaatac aacctaagtc tattaataag ctctgctata caatatagtc gtcatactat
     4381 tagacaagta atgataaaac agtcttctca gactgtttta tttttatttt aaaatcattg
     4441 ttattgtaaa catctcagac catgacaatt tttaaacttc atgccagagc cacaaggaca
     4501 tttatcatta cgtttaatcc cttggtattc tggtgattca ttattcatag gtacatgttg
     4561 tgccgagata ttttgctctg ctgttgtcgt agcatgttga gaagcacgtt cacgctcttg
     4621 ttcatgaatt tgagctttca tcaatgtacg tgtcacatca aactcaatcg aaccaatcat
     4681 atcttggaac atacggaaac cttctgattg atactcaacg attgggttat tttgagcata
     4741 accacgaagt ccaacggagt tacgtaattg atctaaggca tcaatatgct ctgtccattg
     4801 attatccact accatcaaga ttaagacttt ttggaattcg ataatagctt ctggatcttt
     4861 taatttagca atttgttgct cataaacagc taaggcacgt tcataaagaa gttctttaat
     4921 ttcagcctct tttagaccac gaagttctga aactgtgata gcttcttcat ctaataacgc
     4981 cgatcttgca aaattaacaa tagcttctgc tgcagtattt ttatcgcttc tgctatgtgc
     5041 atcaactgct cttttaatcg tacgtttaat catccctttg agctctggtc ctaaatcacg
     5101 ctcagcagta atcacttctc tacggttagc ataaataatc tcacgttgtt cacgcataac
     5161 atcatcgtat tgcaagactt gtttacgtgt gtcatagtta ttaccttcaa cgcggcgttg
     5221 agctgattca acttgtcgtg tcaacatttt tgatttaata actgtatcat cttcagctaa
     5281 attcattcgc tcaagaacca ctttaatacg atctgtacca aagcgacgca tcaaatcatc
     5341 ttctaacgaa agatagaatt gtgattcacc tggatctcct tgacgacccg aacgaccacg
     5401 aagctggtta tcaatacgac ggctttcatg acgctctgta ccaataacac ataaaccacc
     5461 aagttcacga accccttcac caagcttgat atcagtacca cgaccagcca tgttggttgc
     5521 gattgtaaca gcaccacgtt gcccagcatt cataatgatt tgagcttctt taaagtgatt
     5581 tttagcattc aaaacttcat gtggtactcc agcagcaact aattttctag aaattaagtc
     5641 tgacgtttca acagctactg taccaaccag tacaggttgt ccttgttcat aacgttcttt
     5701 gacatctgct accaccgctc taaatttaga gtcaagagtt ggataaagta gatctgaatg
     5761 atctatacgt tgaacaggac gatttgttgg gataggaata acgcgcatat tataaatttc
     5821 acgaaactct tcttcctcag tcttaccagt accagtcatt cctgctaatt tatggtacat
     5881 acggaacata ttttggtaag taattgaagc gctcgttttt gattcttctt ggattggcac
     5941 tgactctttt gcttcaatag cttggtgtaa gccatctgag aaacgacgtc cttccatcgt
     6001 acgaccggta aattggtcca caattaaaat ctcttgttct tcactaacca cataatcaat
     6061 atttaacaac ataatatagt tagctcgaag agcgttatca atgtagtgag ttaaggcaac
     6121 attttcgaga tcatacaagt tatttagatg gaaatagttt tcagccttgt caatacctgt
     6181 atctgataaa ccaattgttt ttgttggaac atcaatgata taatcatcgc tattaagcgt
     6241 ttttacaaac atgtccgcac gtgtatacag ttggttcatc tcagagctta caggacctga
     6301 aacaatcaat ggcgtcctag cttcatcgat taatacagag tcaacctcat caaccaaagc
     6361 atagttgagt gggcgttgca ccatatcttc ttggcgaaca accatattgt ctcgaaggta
     6421 gtcaaatccg acttctgcgt tagttgagta tgtgatatca caattgtaag cttcgcgttt
     6481 ttcaaatggt gacttagctg ctaagttaat ccctacagat aatccaagcc aactataaac
     6541 ctcacccatt tcggtcgcat cacgtgttga taagtactca ttaacagtaa taacatgaac
     6601 acctaatcct gagatagcat ttagataaac aggcattgtc gctgttaaag tttttccctc
     6661 accagtacgc atttctggaa tatcaccatg gtgaagaacg ataccaccca taatttggac
     6721 gtggtatggg tataaaccta atacacgttt agaagcttca cgaactaccg caaacgcttc
     6781 cggtaacaat tgatctaaag tttcaccatt ttgataacgt tctttaaatt ctggagtttt
     6841 tgcttgtaaa gcttcatctg ataacgcagc catatgatct gcgtaagagt caactttctt
     6901 agctatttta tctaattttt ttaattcacc cttgtcattt tcaataacag tgcgtagaat
     6961 atttgccatt ctaattcctt tcgatactct tatcaccaca ttttaccata aataatgttg
     7021 tctttcaata aatggtcgtt ttaatcgaga ttaaaaagag tggtatcccc actctcatta
     7081 ttttttaaca tgacttgcta taatttctaa ctgtccatca atatcccatt tgacaacatc
     7141 atttggcaag ataaagtggt cacctttttt aagtgtataa actttgttat ctactgtaat
     7201 gtgacccgct ccgtctaaga cactaaccaa aaggtagggt gcaaattgtt taaagttggt
     7261 aacaccagaa atttcccatt tataaactgt aaagaaatca tttgaaacta ataaagttga
     7321 agatagatgt tttaacttca tattggcagg aacagtattg gcaggtttac caatcgtgag
     7381 aacatctatt gattgttcaa tgtgtaaatc acgtaatttt cctgaagcat ctggacgatc
     7441 aaaatcataa acacgataag tagtatcact agattgctgt gtttctaaaa ttaatatccc
     7501 tttacctata gcatgcattg tcccacttgg aacgtagtaa aaatcccctg atttaacagg
     7561 aattcgggtt aaaagatgtt cccaatctcc agattcaatc atttggcgaa gttcttcttt
     7621 agttttagca ttatgaccat agataatttc agatccttca tcagcagaaa taatatacca
     7681 acattctgtt ttaccaagtt caccttcatg tttaagagca taagcatcat ccggatgaac
     7741 ctgtacacta agccagtcat tagcatctaa gattttagtt agtaaaggaa agacagtatc
     7801 atcagggttg ccaaataagc ttttttcacc ttgatacagc ttatctaaaa aacaaccttt
     7861 aaagcgacca ttcttaactc ttgagacacc gttaggatga gctgaaatag cccaatattc
     7921 tcctgttgtt tcacttggaa tatcatagcc aaattcatcc cttaactttg ttccacccca
     7981 gattttatcg tgcatgcttg cttctaaaaa taatggttcc gacataagaa tcccctatct
     8041 tttgtaaaat tcaagcctat tatagcattt tatccatcat atcgcttact ttttttaatc
     8101 ttttatttgc taactaaact aagtagtggc ttaagggttt tattaacctt ttgatatttt
     8161 ctttgctaaa gcaaaatttc ctaacgttgc agaaccattt tcttctacag ctggtgtcac
     8221 aatataatca gacaaattgg gaactggtaa atacccattt aataaagttg caaatgtctg
     8281 gcgaacacgt cttaacatgt gtttttgtgc catgacgcct ccaccaaata caatgacttg
     8341 tggacgatat aatacagttg cttgtattgc agcctgagca atataatagg cttgaacatc
     8401 ccaaaccatt gaattttctt caatcaattc accacgaata ccagtcctag cttctagagt
     8461 tggaccagat gccaaacctt ctaaacagct attatgaaat ggacagatac ctttaaaatc
     8521 atttgcttgg tcctgcggat gcatcgccat ataagtatga cctgcttcag tatgaccagt
     8581 tcctcctata aattcaccct tttgtatagc tcctgcacca attcctgtac caatggtgta
     8641 ataaacaagt gaatcaatgt tgttacgagc tataacttca ccataggcag agctattaac
     8701 atctgttgtg aaataaaatg gaacattgaa atctttagaa attaagccga gtaaattaac
     8761 attagcccaa tgtaactttg gggtcgttgt gatataaccg tatgtctttg attttttatc
     8821 aatatcaatg ggaccaaaag aaccaatagc aactgcctct aacttctttt caaatctttt
     8881 aaagaaatca acagtctttt taatcgtttc ttgaggcgtc gttgttggaa attgtatttt
     8941 ttctacaact tttaattcct catcaccaac tgcacaaaca aactttgtcc cacctgcttc
     9001 aatactacca tataactttg tcatcttttt tcctatctat aatctctatc tcaaagacaa
     9061 aggttgggat aattatccca acctttaatc ctcacttaac atcaataatc atgtcacctg
     9121 tagtaacaac accctctttt accaatggta taacatcagc aaaatcagct gtattagtca
     9181 caataatcat agctgtatta tccagacctg cttctgcaat cttggctgaa tcaaaggtgc
     9241 caagaatatc accttttttc actttttggt ctgcctttac tttagaagta aaccctgtgc
     9301 cattcatggt gacagtatca atacctatat gaatcaaaat ttctgcacct ttatctgatt
     9361 tgataccgta agcatgacct gtttcaaaaa caatttggac aaaaccatca acaggtgagt
     9421 aaattgtatt accgcttggt ttaatagcaa ggccatctcc cataatacct gatgaaaaaa
     9481 ctgggtcact tactgtatca agagcaataa cctgtccatc aagagggcta acaatcgttt
     9541 ctgcccttaa ttggctagtt tgagtaattg ttggattgtc atctgtttct tcaaccaaag
     9601 gactaacttc agctactact ttttcatctt tatcttcata tccaaacata taagttaata
     9661 caaaagccaa agccgtagtt ccaatcacca taataaggta cttaacaatt tgaccattta
     9721 aataaagtaa tgttcctggg ataatggtaa taccaaaacc agttcctgct aatttcaata
     9781 ttgaagctaa ccagccacct gctgctcctg caattaatcc cataataaat ggcttaccaa
     9841 aacgtaagtt tactccaaat atagccggtt ctgtaatacc aagtccagct gaaagcgctg
     9901 ccggaaaagc aagggctttt aattttttat tttttgtttt aacaccaact gccaaagtgg
     9961 cacctgcttg tgctgtcata gcagctgtaa taatagcatt aaatggatct ttcccatcag
    10021 cagcaattaa ctgagcttca agtaaattaa agatatgatg aacacctgta acaacgatta
    10081 tctgatgaac accacctaaa ataagacctg ataggccaag aggtaaatta aggacaaatt
    10141 ttgtccctgc taaaacatag ttttcaactg aatggaaaat aggaccaatg acaaaaagtg
    10201 ctaaaattga cataactgta aaagttagga acggcactaa tagtaaatca agaacatctg
    10261 ggattttctt atgcaaccat ttttcaagtt tagcaccaag taaacctaca aagaatgccg
    10321 gcaatactga attttgataa ccaactactg gaataaagcc gaaaaatttt atgggatggg
    10381 catcacctga ggccactgcc caagcgtttg gaagggcaga gttaaccatc atcaatccta
    10441 atacgagacc gataacagga tttccaccaa aaactctaaa agctgaccaa gaaatcaagg
    10501 caggaaaaaa agcaaaggcc gtgtcagtca aaacgacagt ataggtataa aaattagaac
    10561 ttgaaaatgc ttttgaagtc gtcccaaata aagctaaaac tgtatcatta ttaatcgcac
    10621 cgcgaatccc cataaataaa ccagtagcaa caatggctgg caacaatgga acaaaaacat
    10681 caccgaaagt acgaatagct cgttggaaag cattcccttg tttagctgct tctgcctttt
    10741 gatcagaggt tgacgaagtt ggcaaacctt gagccactac ttcatcataa atcttattaa
    10801 cagtaccagt tccaaaaata atttgatatt gacctgaatt gaaaaatgct ccctgaactt
    10861 tttcgatatt ttcaacagta tttttatcaa tgactgtctc atcttttacc ataacgcgaa
    10921 gacgagtggc acagtgtgct acgctacgca cattactagc accaccaatc gcattaatga
    10981 cttgtttagc aatttctgta tttgaagcca tctataaatc tcctttgata ttttgaaagc
    11041 gtttcatttt atttactcta atattctagc acgtctcata aatatatgtc aagcgtttga
    11101 cataaaataa tattttttga tattaagagt tgatttcaaa agagaaaacg tttactatat
    11161 agttattaga tattaagaca gtcaaggagt atcaaatgaa tttacccact gaaatccgct
    11221 atcgtcctta tgatgaatgg actgaagaag ataaggaaaa tattgtaaag aatgtctcaa
    11281 aatccccttg gagagcaact tatcacttag aagctaaaac agggctttta aatgacccaa
    11341 atggcttctc ttactttaat ggaaaattcc atctttttta ccaaaattgg ccctttggag
    11401 ctgcacatgg tttaaaacaa tgggtacata cagaatcaga tgatttagtt catttcaaag
    11461 aaacaggaat caagcttaaa cctgaccatg tcaatgatag ccatggtgca tactccggtt
    11521 ctgcattagc cattgacgat aagctttttc ttttttatac tggtaatgtc cgtgacatga
    11581 aatggaaccg tgacccccgc caaattggag catggatgac aagtgatggc aagattacaa
    11641 aatttgataa ggtccttatt tcacaaccaa atgatgtcac tgagcatttt cgtgaccctc
    11701 agattttcaa ttatgataat caattctatg ctgttattgg ggcgcaaaat agtaaaaaat
    11761 gcggattcat taaattatat aaagcactta ataatgacat ccatcattgg gagtttgtgg
    11821 gagatttaga ttttggtggt actggatctg agtatatgat tgaatgtcca aatattattt
    11881 ttgtcaaagg aaaacctgta ttactttata gtcctcaagg actagataaa aacgaattgg
    11941 actaccaaaa catttatcca aatacctaca aaatcggaca atatttcgat gctaactcat
    12001 ctaagatagt tgaaccatcc cctatctata atttagatta tggttttgaa gcctatgcta
    12061 ctcaaggatt taacacttct gatggtcgtg cctttatcgt gagttggata ggcttgccag
    12121 atattgatta tcctagcgat caatttgatt atcaaggagc tatgagttta gttaaagagt
    12181 taagtataaa aaatgggaac ctttatcaat atcccgttcc tgctatgaaa aatcttcgtc
    12241 aacatcaagc agaatttaaa acacaacttc aaactaacaa cacctatgaa ttagaattac
    12301 tggttcctag aaatgatctt tcttcatttg ttctctttgc taatccgaaa ggacaaggac
    12361 tatccattac tattgatact gcaaagggga aagttatcgt tgaccgctca caagccggac
    12421 agcagtacgc cacagaattt ggtactagtc gccagtgtaa cattccaaaa gatgctacta
    12481 gtgtcaatgt ttttatcgat aagtccatct ttgaaatctt tataaataaa ggagaaaaag
    12541 tatttacggg acgagtcttt ccagatgcag aacaatcagg gattcaacta aaagaaggac
    12601 acgttcatgg taaatatttt gaattaaaat attaatatgg ttgcaaaatt aacagatgtt
    12661 gctgcacttg ccggtgttag cccaacgact gtatcacgcg ttattaacaa aaaaggctat
    12721 ttatctcaaa aaacagtcac caaagtcaat gaagccatga gaacccttgg ttataaacct
    12781 aataatttag cacgtagctt acagggaaag tccgctaaat taattggact cattttcccc
    12841 aacatccgta acatttttta cgctgaatta attgaacacc ttgaaattga gttgtttaag
    12901 catggttata aaactatctt atgtaatagt gaaaaggatc ctatcaaaga aaaagaatac
    12961 ctcgaaatgc taggagcaaa tcaagtagac ggtatcattt caagtagtca caacctagga
    13021 attgatgatt acgagaaagt tgaggctccc atagtagcat tcgatagaaa tttagctcca
    13081 cacatcccta tcgtctcatc tgataatttc tttggaggaa aaatggctgc tcaaaccctt
    13141 aaaaaacatg gctgtcaaaa gatgattatg attacaggga atgataattc agattctcct
    13201 actggactta ggcggctagg tttttcctat gaatcaaagg aatccaaagt tatcacagtc
    13261 acaaatgggc tatctaatat gagacgtgag atggaattaa aatcaattat ctcgactcac
    13321 aaaccagatg gtatttttac atcagacgac ttaactgctt tattagtaat caaacttatc
    13381 agccaactag ggttgtccat tccagaagat atcaaagtga ttggatatga tggtacaagt
    13441 ttcattcaag attacgtccc acatctaacc actattaaac agcctattcg agaaattgcg
    13501 caattgatgg ttgaaatact cctagctaag attgaggggc aaaagacaaa taaggattat
    13561 attttaccag tcagtctcat ccctggtagt agcgtttagg aaatcatatt tatgaaataa
    13621 tttttagtac cgatagttat ttttcaaagc aaattaaaaa tgtggaagaa gattcttcca
    13681 catttttaat tttcatttgt tataaattga cttaataaac cattgacaaa ttttgctgac
    13741 gtctcatctg aatatttttt agcaatctcg ataatctcat ttagagctac acgatcaggc
    13801 gtttcatcaa aatatttaat ttcatagagg cctaagcgta agagagattt atcaaccaat
    13861 gttagacgtt ctaagctcca tcctgatttt aagtgactag aaatcaaagt atctaattca
    13921 tctttgtgat ctacaacacc attaacaaga ttaagtaaaa agattggaac ctctaaaact
    13981 ttgtcttcag atactgtttt atcataacca taagcaaagt gagccgcatc aataaactct
    14041 ccaccagttt ctaagctaaa tagtgtttga aaagcacgtt cacgtaaatc acgtcttgaa
    14101 tctttaaata cactagtcat ctaagaaatc ctcatcaaac aatgacttca agtctggttt
    14161 tggtgttttc tctggaacaa taccttcaac atggatattg accgaagaaa tttttacttc
    14221 tgccatgtca tatacagctg ttttgacagc ttgttgaata gcaattgaaa ctgctggtac
    14281 atttacacca tactgcaagt aaacataaat atctgcagct actgtatcat cttcttcatt
    14341 tttaaggtag acacctctac ctagagattt cttactaagg ctatccgtta ccgctttgtt
    14401 acgtaacgaa tgaacaccat caactttagt tgcagcaata cctgtaatta cctcaagtac
    14461 acgtggtgaa atgacaattt ctccaagatt ttcagttgtc atagcttatc ctttctttta
    14521 gtgtttacta aggtttttcc ttaatagagt aaattcatca tgaatctact taacactttt
    14581 ttgagagtca ttaggcgcgt gaaacgtaag ttccttctgc tgtattgatt actaactttt
    14641 gtccagcttc aataaagtct ggaacgttaa caacaagtcc tgtttccata gttgcaggtt
    14701 ttcctgaacc agttacagta gcacctttga ttgatggttg agtttcagca actgtcaatt
    14761 caacagttgt tggaatttga acaccaataa cttctgttcc atagaattga atttttactt
    14821 ctgagttttc aagaatgtag agtaattcat tttcaatgtt aacagttgga atttcatatt
    14881 gatcgtaagt ttcattattc atgaagtaag ctgtatcatc cattttataa aggtattgag
    14941 caggtacagt ttcaataata gcttgctcaa atttttcttc aggacggtaa cttgtatcaa
    15001 aagttgaacc agtacgaaca tcacgcaatt tcatacgcat gattgtgtta cctttacctg
    15061 gtttgtggtg gctagcttca agaacacgga ttaatttccc atcagccgtt tcaaaagtca
    15121 tgcctgcttt aagcttactt gcttcaatca tttattaata cctctttgta aaaattattt
    15181 atcaactcat tttatcacaa ataacctgct ttctcaaata gtttattgat ttggtatgtg
    15241 tctaatttct tgataaataa ataaaatcac caagagggcg taaataagac tgaagcactt
    15301 gacactttcc tcttacttca gttttgataa tattgccttt tgacgataca atatttttaa
    15361 actgattaag caagtagcat ttgcttcaat cggttggcga gaggtgtttg cacacctttc
    15421 gccttttctt cgtgtttctt taataagatt atgtcacaat agaatattat taaagaaaaa
    15481 atcgtaaatt tattgcctga catcacttat ttttcaaggt ttcagcaagt tttataataa
    15541 ttagtcataa aatgatcttc tagatttggg agatttttca tttctccgta gtgaaggtaa
    15601 attatattct tttaatgtat atttaaaatg atttcctagg tttcttaatc cttcattttt
    15661 gattgcttca tattgtgctc tccattttcc gaaaaataaa tctcttaaat tgccttcatg
    15721 acttaaaagg attgtttagc tcataacttc cgaaccattt ctactccaat acattcctct
    15781 atttttcagt agctgccttc taaatctctg aaaagaagac agtttacttt ctgattcaat
    15841 taatgattct tctgtatcta ataccgtttt aagcgatttt ttacttcttt tcttgattac
    15901 tttaatggct agattcttta actctgatgg taagttagaa aaattcactt tgataacccg
    15961 attaatatga aacgtatccc aaaaatgata gtgttcattg gtttaaaccc actgactaat
    16021 tctttaaaat attgtgatga atacccaagt ccacccgtct gaattagtaa taaaagtagt
    16081 ctcaggggtg ttctcataat gattatacaa ctgatcaata agttgttctt tttttaaatg
    16141 tatttaattg tttactagag tatagagaaa gatataggtc ttttagttaa taattgttcc
    16201 ttcatgtctt gctgcattta acacctcctc ctttaagtcc acacaatact aatactcttt
    16261 ataaaaacta ttccatatac tttttataac ttcattttct cttaatctaa aacatatgca
    16321 aaattatttt taatattcat attcacttgc caattgagag cattcaacta attttctgta
    16381 tctcgtcgaa ttttccaata aatagctatg agttccaaaa ttctctacct ttccgttttc
    16441 aataactata atcttatttg cgttttctat agacttcatt ctatgagaaa cgataataac
    16501 tgttttattt tttataagct tatctaaacc ggcttgtatc tttatttcat tttctatatc
    16561 taaactggca gagatttcat caagcagtat tattggtgca tctttaagaa tggctcttgc
    16621 aatagaaatt ctttgtcttt cgcctcctga taatttactc ccgttttcac ctatttttgt
    16681 gtcatattta tctacgaatt tatcagcaat ctcatctaca tttgcaattt tcgcagcttc
    16741 taacacttct tcgatagtag ctttgggatt tccacattta atgttttcaa aaatagtatc
    16801 atcaaataac tcaacatctt ggaagactgt tgaaatattt tcaaataaac tatttgttga
    16861 tatttcagaa atatctttcc cgcctatcaa tatttgtccg gcattatagt cgtataatct
    16921 tgatagtagt tttaaaatag ttgactttcc acaaccggag ggccctacta tcgcagtaac
    16981 ttctccttga tttgcattga aagataaatt acttacaaca tttcttcctt tctcataagc
    17041 aaagctaaca tccctaaatt caacattgaa tgtctgaata tctgcattta ccccttcttg
    17101 taaaggagtc tttcttattg ccgtaacttg tttaaaactg ctttgaaagt ttattatcat
    17161 taaaagactg tctaacatgc ccgtaaaacc actggctacc tttgtggcac aaattatata
    17221 tcctatatag aagattaact gtatttgacc agtcaaatac agttgaaacc cgaaaataat
    17281 aaccagagct atcgagaact gtggcaaaat tgataataag cttgatggta tcgttgtaaa
    17341 cagctctgtt tttatctgta ttttttcact gtctttcaat ttgtcttgta catcttttct
    17401 cactgtatgt tgcatgttat aagattttat atcttgttgc atctcaaaag aatcttgaaa
    17461 tgcctgagaa ttctctctca gttttttata atactttcta tttattctat attgaatatt
    17521 tttcgacaga tacaatatca gcaatgcagt tattacaggt acaataatac atagtcctaa
    17581 ttgtaaagat ccaattaaaa ggctcaaact tagaaaaaca aaataaccaa tccatccata
    17641 atacgcacat atagcatgag ataaggcttg ttcgattttt gacacatcag ccattactgt
    17701 ttgcgataaa tctgtcaaat catgtgtt
//
LOCUS       DATRTN010000034        16614 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.034, whole genome shotgun sequence.
ACCESSION   DATRTN010000034 DATRTN010000000
VERSION     DATRTN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16614
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.034"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(34..282)
                     /locus_tag="VBE22_001117"
     CDS             complement(34..282)
                     /locus_tag="VBE22_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEO7202584.1"
                     /translation="MEVKTLANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANP
                     SEELYRAASSSIDKAASKGLIHTNKASRDKARLATKLG"
     gene            complement(336..1256)
                     /locus_tag="VBE22_001118"
     CDS             complement(336..1256)
                     /locus_tag="VBE22_001118"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEO7202585.1"
                     /translation="MNNEFINFDRISRENWKDLHQQSQALLTEKELESIKSLNDNINI
                     QDVIDIYLPLINLIQIYKRSQENLSFSKAIFLKKENYQRPFIIGISGSVAVGKSTTSR
                     LLQLLISRTFKDSHVELVTTDGFLYPNEKLIQNGILNRKGFPESYDMESLLNFLDTIK
                     NGITAKIPIYSHEIYDIVPNQLQTIETPDFLILEGINVFQNQQNHRLYMNDYFDFSIY
                     IDAENKQIEEWYLQRFNSLLQLAEADPSNYYHKFTQIPPHKAMELAKDIWKTINLVNL
                     EKYIEPTRNRADFIIHKGKHHKIDEIYLKK"
     gene            1362..1952
                     /locus_tag="VBE22_001119"
     CDS             1362..1952
                     /locus_tag="VBE22_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7202586.1"
                     /translation="MANMYYTENPNVEHDIHELNVKLLGESFSFLTDAGVFSKRMIDY
                     GSQVLLNSLHFEKNKSLLDLGCGYGPLGISLAKVQGVKATMVDINTRALELAKKNATR
                     NGVVVEVFQSNIYENISKTFDYIISNPPIRAGKQVVHSIIEESICYLNTGGSLTIVIQ
                     KKQGAPSAKAKMLDTFGNCDILKKDKGYYILRSEKV"
     gene            2278..2667
                     /locus_tag="VBE22_001120"
     CDS             2278..2667
                     /locus_tag="VBE22_001120"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEO7202587.1"
                     /translation="MGNIELKKLAVKASENAYVPYSKFPVGAALRTAEGKIFTGCNVE
                     NISYGLANCAERTAIFKAVSEGYKDFSEIAIYGNTEEPISPCGACRQVMVEFFNKNAK
                     VTLIAKNGKTVETTVGELLPYSFVDLK"
     gene            2732..3781
                     /locus_tag="VBE22_001121"
     CDS             2732..3781
                     /locus_tag="VBE22_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO7202588.1"
                     /translation="MNKKIAGIGLASIAVLSLAACGHRGASKSGGKSDSLKVAMVTDT
                     GGVDDKSFNQSGWEGMQAWGKKNGLKKGAGFDYFQSASESDYATNLDTAVSSGYKLIF
                     GIGFSLHDAIDKAADNNKDVNYVIVDDVIKGKDNVASVVFADNESAYLAGIAAAKTTK
                     TKTVGFVGGMESEVITRFEKGFEAGVKSVDKSIKIKVDYAGSFGDAAKGKTIAAAQYA
                     SGADIVYQVAGGTGAGVFSEAKSRNESLKEADKVWVLGVDRDQAAEGKYTSKDGKASN
                     FVLASSIKEVGKSVELIATKTSKGKFPGGNVTTYGLKDGGVDIATTNLSDDAVKAIKE
                     AKAKIISGDIKVPSK"
     gene            3926..5461
                     /locus_tag="VBE22_001122"
     CDS             3926..5461
                     /locus_tag="VBE22_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202589.1"
                     /translation="MTQNVIEMKEITKKFGDFVANDHINLTVEKGEIHALLGENGAGK
                     STLMNMLAGLLEPTDGQIFINGQPVTIDSPSKSSQLGIGMVHQHFMLVEAFTVAENIV
                     LGNETTQNGVLDIKTAAKEIKELSEKYGLSVNPNAKISDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPSEIKELMTIMKSLVKEGKSIILITHKLDEIRAVADKVTVIRRGK
                     SIETVPVAGASSQQLAEMMVGRSVSFRTEKKEAKPTDIILSVKDLVVEENRGGVLAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVTSGQIVIKGKDVTKFSSRQITEL
                     SVGHVPEDRHRDGLVLDMTMAENLALQTYYKEPLSHKGILNFAKIKEYARQLMTEFDV
                     RGAGEHVLARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DKGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVKPDQTNKQELGILMAGGKIEKEE
                     RDV"
     gene            5454..6515
                     /locus_tag="VBE22_001123"
     CDS             5454..6515
                     /locus_tag="VBE22_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7202590.1"
                     /translation="MSKKAQKIAVPLISVVLGIILGAIIMLIFGYDPLWGYEGLFQTA
                     FGSIKNIGEIFRAMGPLILIALGFSVASCAGFFNIGLPGQALSGWIAAGWFALSHPDM
                     PRPAMILCTIIIGIVAGGITGAIPGILRAYLGTSEVIVTIMMNYIVLYSGNSIVQRVF
                     PKSIMRTSDSSVYVSANASYQTDWLSSLTNNSRINIGIFIAIIAVVLVWFLLNKTTLG
                     FEIRSVGLNPNASEYAGMSAKRTIILSMIISGAFAGLGGVVEGLGTFENVFVQPSSLA
                     IGFDGMAVSLLAANSPIGILFAAFLFGVLSVGAPGMNIAGIPPELIKVVTASIIFFVG
                     VHYIIEYVIKPKKQMKGGK"
     gene            6517..7473
                     /locus_tag="VBE22_001124"
     CDS             6517..7473
                     /locus_tag="VBE22_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7202591.1"
                     /translation="MSLTTIFALLFSSMLIYATPLIFTSIGGTFSERGGIVNVGLEGI
                     MVIGAFSGVVFNLEFASVFGDATPWISVLVGGLVGLIFSVIHAVATVNFRADHIISGT
                     VLNLMAPSLAVFLIKVLYNKGQTDNIQESFGKFNFPILSDIPFVGDIFFKGTSLVGYI
                     AILFSFLAWFILYKTRFGLRLRSVGEHPQAADTLGINVYLMRYSGVLISGFLGGIGGA
                     VYAQSISVNFAATTILGPGFISLAAMIFGKWNPIGAMLASLFFGLSQSLAVIGSHLPL
                     LSNIPTVYLQIAPYVLTIIVLAAFFGQAVAPKADGINYIKTK"
     gene            7679..9049
                     /locus_tag="VBE22_001125"
     CDS             7679..9049
                     /locus_tag="VBE22_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO7202592.1"
                     /translation="MSKIVVVGTNHAGTAAIKTMLSNYGEANEIVTFDQNSNISFLGC
                     GMALWIGEQIDGPEGLFYSDKEQLESMGAKVYMNSPVLNIDYDKKEVTALVDGKEHVE
                     SYEKLILATGSQPIIPPIKGVEIQEGSREFKATLENLQFVKLYQNSEEVIEKLAKPGI
                     NRVAVVGAGYIGVELAEAFQRIGKEVTLVDVADTCMGGYYDRDFTDMMSKNLEDHGIR
                     LAFGQAVQAVEGDGKVERLVTDKETFDVDMVILAVGFRPNTELGAGKLDTFRNGAWVV
                     DKKQETSVKDVYAIGDCATIWDNSRDDINYIALASNAVRTGIVAAHNACGTELEGAGV
                     QGSNGISIYGLNMVSTGLTLEKAKQAGYNAVETGFNDLQKPEFIKHNNHEVAIKIVYD
                     KDSRVILGCQMVSHEDVSMGIHMFSLAIQEKVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(9169..10158)
                     /locus_tag="VBE22_001126"
     CDS             complement(9169..10158)
                     /locus_tag="VBE22_001126"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO7202593.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPALF
                     DKAVGDAEDLSHALAFTSPKKIYAATYADCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALADKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVQLEQWLQENRDIDEQGLVDLF
                     ISVRDAAYSIINKKGATYYGIAVALARITKAILDDENAVLPLSVYQEGQYGDVKDVFI
                     GQPAIVGAHGIVRPVNIPLNDAELQKMQASAEQLKDIIDEAWKNPEFQEASKN"
     gene            10397..12856
                     /gene="gyrA"
                     /locus_tag="VBE22_001127"
     CDS             10397..12856
                     /gene="gyrA"
                     /locus_tag="VBE22_001127"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEO7202594.1"
                     /translation="MQDKNLVDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVDFQDNYDGSEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGESIDAVKLVMDNPDVTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYETGKGSIVLRSRTEIETTSNGKERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRIEGITAVRDESSREGVRFVIEVRRDASANVILNNLFKLTSLQTNFSFNMLAI
                     EKGVPKILSLRQIIDNYIEHQKEVIVRRTQFDKAKAEARAHILEGLLVALDHLDEVIT
                     IIRNSETDTIAQAELMSRFELSERQSQAILDMRLRRLTGLERDKIQSEYNDLLALIAD
                     LADILAKPERVVTIIKEEMDEVKRKYADARRTELMIGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGIQGTGVNNDDFVRELVSTSTHDTVLFFTNLGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLDEGETIQTIINARKEDVANKYFFFTTQQGIVKRTS
                     VSEFSNIRQNGLRAINLKENDELINVLLIDENEDVIIGTRTGYSVRFKVNAVRNMGRT
                     ATGVRGVNLREGDKVVGASRIVNGQEVLIITEKGYGKRTEASEYPTKGRGGKGIKTAN
                     ITAKNGPLARLVTINGNEDIMVITDTGVIIRTNVANISQTGRSTMGVKVMRLDQEAKI
                     VTVALVEQEIEDKSNIEDTKE"
     gene            12863..13606
                     /locus_tag="VBE22_001128"
     CDS             12863..13606
                     /locus_tag="VBE22_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEO7202595.1"
                     /translation="MRNKKKLHGFFNFVRWLLVVLLIIVGLALVFNKPIRNAFIAHQS
                     NHYQISRVSKKTIEKNKKSKTSYDFSSVKSISTESILSAQTKSHNLPVIGGIAIPDVE
                     INLPIFKGLGNTELSYGAGTMKENQIMGGQNNYALASHHVFGLTGSSKMLFSPLEHAK
                     KGMKVYLTDKSKVYTYTITEISKVTPEHVEVIDDTPGKSQLTLVTCTDPEATERIIVH
                     AELEKTGEFSTADESILKAFSKKYNQINL"
     gene            13622..14035
                     /locus_tag="VBE22_001129"
     CDS             13622..14035
                     /locus_tag="VBE22_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7202596.1"
                     /translation="MKLKAVHHIAIIVSDYEKSKDFYVNKLGFEIIRENHRPERHDYK
                     LDLRCGDIELEIFGNRLDDPEYETPPQRIGRPNWPREACGLRHLAFYVPDVEAYKIEL
                     ENLGIFVEPIRYDDYTGKKMTFFFDPDGLPLELHE"
     gene            14189..15151
                     /locus_tag="VBE22_001130"
     CDS             14189..15151
                     /locus_tag="VBE22_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEO7202597.1"
                     /translation="MSIKKLLFAGLAFILFTLASPAYAASDVQKVIDETYVQPDYVLG
                     YSLNQEQRAQTLQLLNYDESRDTKVKTLNTSSYAKIMNIADDASIQLYSSVKIKKLGS
                     NDTLAVNIVTPENITKVTEDMYRNAAVTLGIEHATISVAAPIKVTGESALAGIYYSLE
                     KNGASVSSENKQLAQEELSTLSGINAENKGKEGYDADKLNVALTDIKSAVAKGGSDLS
                     KDDIRKIVEETLKNYHLDNAVTENQINLIVNFAVNLSQSNVIKNSDFTNTLNNLKDNI
                     VSKAGSKFKNINVNFNANKAVESGKGFLANIWQQIVNFFQQFGS"
     gene            complement(15215..16342)
                     /locus_tag="VBE22_001131"
     CDS             complement(15215..16342)
                     /locus_tag="VBE22_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO7202598.1"
                     /translation="MHIIIQITIILLASVLATLISKRIGIPAVVGQLLVGIIIGPAML
                     GLVHQNQVLHVLSEIGVILLMFLAGLEANFDLLKKYLKPSLLVAITGVIVPMALFYFL
                     TRLFGFQINTAIFYGLVFAATSISITVEVLQEYNRVKTDTGAIILGAAVADDVLAVLL
                     LSVFIATNGSSSNIGLQIIIQLLFFVFLFICMKYLVPALFKLIEKVHFFEKYTILAIL
                     ICFSLSILADKVGMSSIIGSFFAGLAIGQTSFVDKVEHKISLLSYTFFIPIFFASIAL
                     PLKFDGMMSHLHTILIFTALAVLSKLIPGYFVGRGFNFSKLESLTIGGGMVSRGEMAL
                     IIVQVGLAAKIISSTTYSELVIVVILSTIIAPFILKYSFKD"
BASE COUNT         5499 a         2511 c         3300 g         5304 t
ORIGIN      
        1 aaacatcgat atatccgatg ttttctaggt taattaacca agtttagttg caagacgtgc
       61 tttatcgcgg cttgctttgt ttgtgtgaat taaaccttta gaagcagctt tgtcgatgct
      121 tgaagaagca gcgcggtaaa gttcttctga agggttagct tcaaatgctt taatagcagt
      181 acgcatagct gatttttgtg ctgagttttt ttcgttttgt ttaacgttaa gttcagcgcg
      241 tttgatagct gatttaatat ttgccaatgt tttcacctcc atattaatct aactatataa
      301 gtatatctaa ttaagtgatt tttggcaacc ctttatcact ttttgagata aatttcatca
      361 attttatgat gtttcccttt atgaataata aaatcagccc tatttctggt tggttctata
      421 tatttttcaa ggttgactaa attaatagtt ttccaaatat ctttagctag ttccatagct
      481 ttatgtggcg gtatttgtgt gaatttatga tagtaattac tagggtctgc ttcggctaat
      541 tgtagaagac tgttaaatcg ttgtaaatac cattcttcaa tttgtttatt ttcagcatcg
      601 atgtatattg aaaaatcaaa ataatcattc atgtaaagtc gatggttttg ttggttttga
      661 aagacattta ttccttctaa aattaaaaaa tctggtgttt caatagtttg caattgattt
      721 ggaacgatat cgtagatttc atgagaataa attgggattt tcgcggttat accgttcttt
      781 atagtatcta agaaatttag aagtgactcc atatcatagg attccgggaa acctttacgg
      841 tttaaaattc cattttgtat gagtttctcg ttaggatata aaaagccatc agtagtgact
      901 aattcgacgt gactatcttt aaaagttcgt gaaattagta attgtagtaa gcgactggta
      961 gttgattttc caacagcaac tgaccctgat atgccgataa taaaaggacg ttgataattt
     1021 tcctttttaa gaaatattgc tttagaaaaa gataaatttt cttgactacg tttatagatt
     1081 tgaataaggt taattaaagg aaggtagatg tcaataacat cttgaatatt aatattatcg
     1141 tttaagcttt tgatagattc aagctctttt tcagttagta aagcttggga ttgctgatgt
     1201 aagtccttcc aattttctct ggaaatacgg tcgaaattaa taaattcatt attcatatca
     1261 aactcctata tgaaagctat tataacattt tattgagtga ttgaaaaccg tttcaaaata
     1321 tggtaaaata atatggcgaa attggagggt tagaattatt tatggctaat atgtattata
     1381 cagagaatcc taatgttgaa catgatattc atgagctaaa tgtcaaatta ttaggagaat
     1441 cattcagttt tttaactgat gcaggagttt tctctaaaag aatgatagat tatggtagtc
     1501 aagtccttct aaatagttta cattttgaaa aaaataaatc cttgttagat cttgggtgtg
     1561 gatacggtcc gctgggaatt tctctagcca aagtgcaagg agttaaagcg actatggttg
     1621 acattaatac gagagcattg gaattagcta agaaaaatgc aacacgcaat ggagtagtag
     1681 ttgaagtctt tcagtcaaac atttatgaaa atatttctaa aactttcgac tatattataa
     1741 gcaacccccc tattcgtgct ggaaagcaag ttgttcattc tattatcgaa gaaagtatct
     1801 gttaccttaa tacaggcggt agccttacca ttgtcattca aaagaaacaa ggcgctccaa
     1861 gtgcaaaagc taaaatgcta gatacttttg gtaattgcga tattttaaaa aaagataagg
     1921 gatattatat attgagaagt gaaaaagtct aaatgatatc cctaaagcgt aaaaataata
     1981 aatagttcgg ataataacta tcgtattatt ttttggaaag gttttctcta aaaatgtttt
     2041 ctttaaattc cgtataagat aaaaagaatt ctgcttttta ttctgaaaat tataaaaaac
     2101 tacttacatt atctaaaaaa aatggtaaaa tagattcagc taaaatacca agactaatga
     2161 aaccacaaga cttggttact caataataaa tacttctgtg tttgataaga agataataaa
     2221 cgtgttctaa aaattttgcc agtaaacatt atgaagcgaa aggtgcagct actaaaaatg
     2281 ggaaatatag agttgaaaaa attggctgta aaagcgagtg aaaatgcata cgttccttac
     2341 tcgaaatttc cagtaggcgc agcattgcgt acagcagaag gaaaaatatt tacaggatgt
     2401 aacgttgaaa atatcagtta tggattagca aactgtgctg agcgtactgc aatttttaaa
     2461 gctgtttcag aaggttataa agatttttcg gaaattgcta tttatggaaa tactgaagaa
     2521 ccaatttctc cttgcggtgc atgccgtcaa gtgatggtgg aattttttaa taagaatgct
     2581 aaagttacat tgattgctaa aaatggtaag acggtcgaga cgacggtcgg tgagttgtta
     2641 ccatactcgt ttgttgattt aaaataattt aaatcagttg ctcttttaag catttagctt
     2701 aaatattaag ttttttagga gggttcattt catgaacaaa aaaattgccg ggatcggctt
     2761 ggcttcgatt gcagtactta gtttagctgc atgtggacat cgtggtgctt ctaaatctgg
     2821 tggtaaatca gatagcttga aggttgcaat ggtaacagat accggtggtg ttgatgataa
     2881 atcatttaac caatctggtt gggaaggtat gcaagcttgg ggcaagaaga atggccttaa
     2941 aaaaggagct ggttttgact atttccaatc ggcaagtgaa tctgattatg caactaactt
     3001 agatacagct gtgtctagtg gttataaatt gattttcggt attggatttt ctcttcatga
     3061 tgctattgat aaagcagcag acaataacaa agatgttaat tacgtcatcg ttgatgatgt
     3121 tattaaaggg aaagataatg ttgcaagtgt tgtctttgcg gataatgaat cagcttactt
     3181 agcaggtatt gcagccgcta aaactaccaa aacaaaaaca gttggctttg taggtggtat
     3241 ggaatctgag gttattaccc gttttgaaaa aggttttgaa gcaggtgtca aatcagttga
     3301 taaatcaatt aaaattaaag ttgactatgc tggttcattc ggtgatgctg ctaagggtaa
     3361 gacaattgca gccgcacaat atgcttctgg cgcagatatt gtttatcaag tagctggtgg
     3421 aactggtgca ggtgtattca gtgaagcaaa atcacgcaat gaatctctaa aagaagctga
     3481 caaagtttgg gtattaggtg tagaccgtga ccaagcagca gaaggtaagt atacttctaa
     3541 agatggtaag gcttctaact ttgttttggc ttcatcaatt aaagaagttg gaaaatcagt
     3601 tgaattgatt gctactaaaa caagtaaagg taaattccca ggtggtaatg taacaactta
     3661 tggtttgaag gatggtggag ttgatatagc aactacaaac ctttcagatg acgccgttaa
     3721 agcgattaaa gaggctaaag ctaaaattat ttcaggagat atcaaagttc ctagcaaata
     3781 atatctttaa gaactttcaa aaaagcgatt agaaatcgct tttttgaagc tgagataaac
     3841 ttatatctca gtaaaatcca tcaatcgtta aaggttggtg gattttactg aactataaat
     3901 gaatttttct gaaagggaga accacatgac acagaatgtc attgaaatga aagaaatcac
     3961 taagaaattc ggtgattttg tggcaaatga tcatatcaat ttgacggttg aaaaaggtga
     4021 gatacatgct ctgcttggcg aaaacggtgc aggaaaatca actttaatga acatgttagc
     4081 aggtttacta gaaccaacag acggtcaaat ttttattaat ggtcagcctg taactattga
     4141 ttcaccttcc aaatcgtcac aattggggat tggtatggta catcagcatt tcatgcttgt
     4201 tgaagcattt actgtagctg aaaatattgt tctaggaaat gaaaccaccc aaaatggtgt
     4261 tttagacatt aagacagcag ctaaagaaat aaaggaacta tcagagaaat atggtttgtc
     4321 agtaaatcca aatgctaaaa tatctgatat ttctgtagga gcacagcagc gtgttgaaat
     4381 tttaaagaca ctttatcgcg gtgcagatat tttaattttt gatgagccga cggcggtact
     4441 tacaccatca gaaataaaag aattgatgac aattatgaag agccttgtta aagaaggcaa
     4501 atccattata ctaattaccc ataaattgga tgaaattcgg gcagttgctg ataaagttac
     4561 tgtgattcgc cgtggtaaaa gtattgaaac agtccccgtt gcaggcgcaa gttctcaaca
     4621 actggcagaa atgatggttg gacgatctgt ttccttcaga actgaaaaaa aggaagctaa
     4681 accgactgac ataatccttt ctgtaaagga tttagttgta gaagaaaata gaggcggcgt
     4741 tctagctgtt aaaaatctgt cgttagatgt tcgggcaggt gaaattgtag gtatagctgg
     4801 tattgatggt aatggacaaa gtgaattaat tcaagctatt acaggcttaa gaaaggtaac
     4861 ttctggtcaa attgtaatta aaggtaaaga tgtcacaaaa ttttcaagtc gacaaattac
     4921 agaattaagt gtaggtcatg ttccagaaga tcgtcataga gatggtttgg ttttagatat
     4981 gacgatggca gaaaatttag ctttacaaac ttattataaa gagccattga gtcataaagg
     5041 catccttaat tttgctaaaa ttaaagagta tgctcgtcaa ttaatgacag aatttgatgt
     5101 gcgtggtgct ggtgaacatg ttttagcaag aggattttct ggtggtaatc aacaaaaagc
     5161 tattattgca cgtgaagtgg atcgcgatcc agacctactc attgttagcc aaccgactcg
     5221 tggtctagat gttggtgcta tcgagtatat tcataaacgt ttgattgaag agcgcgataa
     5281 agggaaagcg gtcttagtag ttagctttga gttagatgaa atcttaaact tatcagatcg
     5341 tattgcagtt attcatgatg gtaaaattca aggaattgtt aaacccgacc aaaccaacaa
     5401 gcaagaattg ggtattttga tggctggtgg aaaaattgaa aaggaggaga gagatgtcta
     5461 agaaggcgca aaaaattgca gttcctctga tttcagttgt actaggaatc attctaggag
     5521 ctattattat gctgatattt ggttatgatc cactttgggg ttatgagggg ttgttccaaa
     5581 cagcattcgg tagtataaaa aatattggtg aaattttccg tgcaatggga ccattaattt
     5641 taattgctct aggtttctca gttgccagtt gtgcaggttt ctttaatatt ggacttccag
     5701 ggcaagcttt atcaggatgg attgccgcag gttggttcgc tctttcacac ccagatatgc
     5761 cacgtccagc aatgattctt tgtactatta ttattggtat cgttgctggg ggaatcactg
     5821 gagctattcc aggtattctc agagcatatc ttggaacaag tgaagttatt gtaactatta
     5881 tgatgaacta tattgttctt tattcaggaa attctattgt acaacgtgtt ttcccaaaaa
     5941 gcattatgcg tacaagtgac tcgagtgtat atgtttctgc caatgcatca taccagacag
     6001 attggttatc tagtttaacg aataattcac gtatcaatat tggtatcttc attgctatta
     6061 ttgcagttgt gttagtttgg ttcctattaa ataaaacaac gcttggattt gaaattcgtt
     6121 cagttggctt aaatccaaat gctagtgagt acgctggtat gtctgcaaag cgtacaatta
     6181 ttctatcaat gattatttct ggagcttttg caggacttgg tggtgttgtt gaaggattag
     6241 gtacatttga gaatgtattt gtacagccat cctcgttagc tattggcttt gacggtatgg
     6301 cagtaagtct actcgcagcg aatagtccaa taggtatttt atttgcagca tttctatttg
     6361 gtgttctaag tgttggtgcg cctggtatga acattgcagg tattccacct gagttaatta
     6421 aagttgtaac agcttcaatc atcttcttcg taggtgtcca ttacattatt gagtatgtta
     6481 ttaaacctaa aaaacaaatg aaaggtggta agtaaattga gtttaacaac gatttttgca
     6541 ttgctatttt catcaatgct aatttacgca acacctctta tctttacaag tattggggga
     6601 accttctctg aacgtggtgg tatcgtcaac gttggtttag aaggaattat ggtaattgga
     6661 gctttctcag gcgttgtatt taatttagag tttgcttctg tttttggtga tgcgactcca
     6721 tggatttctg tactagttgg tggcttagtt ggtttaattt tttctgttat ccatgccgtt
     6781 gcgacagtta atttccgtgc agatcatatt atcagcggaa ccgttcttaa tttaatggca
     6841 ccttcattag ctgttttctt gattaaggtt ctttataata aaggtcaaac tgataatatt
     6901 caagaatcat ttggaaaatt taatttccct atactatcag atattccttt tgttggtgat
     6961 atcttcttta aaggaacaag cttagtcggt tatattgcta ttcttttctc attccttgct
     7021 tggtttattc tatataaaac tcgctttgga ttaagattac gctcagttgg agaacatcct
     7081 caagcagcag acaccttagg tattaatgtt tatcttatgc gttactctgg tgtcttgatt
     7141 tcaggtttcc ttggtggaat aggaggagct gtatatgctc aatctatttc tgttaacttt
     7201 gcagcgacaa ctatattagg accgggcttt atctccttag cagcgatgat ttttggtaag
     7261 tggaatccaa ttggtgcaat gcttgctagt ctattctttg gtttatcgca aagtttagca
     7321 gttatcggta gccatttacc acttttgagc aatattccaa cagtttatct acaaattgct
     7381 ccatatgttc tcactatcat tgtattagca gctttctttg gacaagcagt agctcctaag
     7441 gcggatggta ttaattatat taaaacgaaa taaataaaaa gaaactagta taagctagtt
     7501 tctttttatt gtttgtgaat ttgtgaaaaa aatactttgt gaaaaaatca cataaattat
     7561 aatacaataa caataaaata taaaaaaata gaaaattgag tcattattct agtaagagtt
     7621 tgtgaataat gttataatgt gttttgttat taattattta taaaggagaa ctcttaatat
     7681 gagtaaaatc gttgtcgtag ggacaaacca cgctggtaca gcagcaatta aaacaatgct
     7741 tagtaattat ggtgaagcca atgaaattgt aacatttgat caaaattcca atatctcttt
     7801 cttaggatgt ggtatggctc tttggattgg tgaacagatt gatggtccag aaggattgtt
     7861 ctactctgat aaagagcagt tggaaagtat gggagctaag gtttacatga actctccagt
     7921 acttaatatt gactacgata aaaaagaagt tacagcttta gtagatggaa aagaacatgt
     7981 tgaaagttat gaaaaactta ttttagcaac tggttcacaa cctatcattc caccaattaa
     8041 aggtgttgaa attcaagaag gatctcgtga atttaaagct actcttgaaa accttcaatt
     8101 tgtgaaactt tatcaaaatt cagaagaagt tattgaaaag ttagctaaac ctggtatcaa
     8161 tcgtgttgct gttgttgggg ctgggtatat tggtgtagaa ttagcagaag ctttccaacg
     8221 tattggaaaa gaagtaacac ttgttgatgt tgctgatact tgtatgggtg gttactatga
     8281 tcgtgacttt actgatatga tgagtaaaaa ccttgaagac catggtattc gtcttgcttt
     8341 cggacaagct gttcaagcag ttgaaggtga tggtaaagta gaacgtcttg ttactgataa
     8401 agaaacattt gatgttgata tggtgatctt agctgttgga ttccgtccaa atacagaact
     8461 cggtgcaggt aaattagata cattccgtaa tggcgcttgg gtagttgata agaagcaaga
     8521 aacaagcgtg aaagatgttt atgctatagg tgattgtgcg acaatttggg ataattctcg
     8581 tgatgatatc aattatattg ctcttgcttc aaacgccgtt cgtactggta ttgtagcagc
     8641 tcacaatgca tgtggtacag agcttgaagg tgctggtgtc caagggtcaa atggtatctc
     8701 aatctatggc cttaacatgg tatcaactgg tttgacttta gaaaaagcaa aacaagctgg
     8761 gtacaatgca gttgaaacag gctttaatga ccttcaaaaa ccagaattta tcaaacacaa
     8821 taatcatgaa gtagctatta agattgtcta tgataaagat agccgagtta tccttggttg
     8881 ccaaatggtc tcacacgaag atgtatctat gggaattcat atgttctcac ttgctattca
     8941 agaaaaagtt actattgaaa aacttgcttt gaccgatatc ttcttcttac cacattttaa
     9001 taagccatat aactatatta caatggctgc attaggcgct aaagattaat ttaaaattaa
     9061 aaaggttaag cgaaatgctt agccttttat ttcatccgtt aacaaggtca aaaatgtaac
     9121 ggcaaagaaa aagctaagat attcctatcc tagctttttt caactatgtt agttttttga
     9181 tgcttcttgg aattctgggt ttttccaagc ttcgtcaata atatctttta attgttcagc
     9241 tgaggcttgc attttttgaa gttcagcatc atttaatggg atattaactg gacgaacgat
     9301 accatgtgca cctacgattg caggctgacc gataaagaca tctttaacat caccgtattg
     9361 gccttcttga tatacagata atggaagaac tgcattttca tcatcaagga tagctttagt
     9421 aatacgagca agtgcgacag caataccgta atatgtagca ccttttttgt tgatgattga
     9481 gtatgcagcg tcacgaactg aaataaacaa atcaacaagt ccttgttcat caatatcgcg
     9541 attttcttgt aaccattgtt caagttggac accagcaacg ttagcatgtg accaaacggc
     9601 aaattctgag tcaccgtgtt cacccatgat gtatgcgtga actgaacgag catcaacacc
     9661 aattttatct gctaaagctt gacggaaacg tgcagagtca agtgaagtac ctgaaccaat
     9721 aacgcgttcc ttagggaaac ctgagaattt ccaagttgaa tatgtaagta catctacagg
     9781 gttagccgct actaagaaga taccgttgaa acctgattca acaacttgag ttacgattga
     9841 tttgttaatt gctaagtttt taccaacaag gtcaagtcga gtttcacctg gtttttgagg
     9901 tgcgccagca gtaatgacaa caaggtcagc atctgcgcag tctgcataag tagctgcgta
     9961 gattttttta ggtgatgtaa atgcaagggc atgtgaaaga tcttcagcat caccaacagc
    10021 tttatcaaat aaagctggga tttcaatgat acctaactct tgcgcaatac cttggttaac
    10081 aagtgcaaaa gcgtaagaag aacctacggc accatcacca acgaggataa cttttttatg
    10141 ttgtttagtt gcagtcattt ctaaacatct ccttaaatat ttttgggaag tttcccatac
    10201 acctatattc tatcattttc aatgaaagaa gtcactaata aaatgtgaaa aaatttgcaa
    10261 acaaattgaa atatcataga aaactattga aaacgctgag tgtcttgata tttttgatac
    10321 cgatttatca tcgcaatttt acctaaatta tggtacaatg taagagactt acagtgaaga
    10381 aaaggggcat tttttaatgc aagataaaaa tttagtagat gttaatctaa ctagtgaaat
    10441 gaaaacgagt tttatcgatt acgccatgag tgtcattgtt gctcgtgcac ttccagatgt
    10501 tagagatggt ttaaaacctg ttcatcgtcg tattttatat ggtatgaatg aactaggtgt
    10561 gacacctgat aaacctcata agaaatcagc acgtattact ggtgatgtta tgggtaaata
    10621 ccatccacat ggtgattcat ctatttacga agcaatggtg cgtatggcac aatggtggtc
    10681 atatcgtcat atgctcgttg atggtcatgg aaactttggt tcaatggatg gggatggtgc
    10741 tgccgcacag cgttatacag aagcacgtat gagtaaaatt gctcttgaga tgcttcgtga
    10801 tatcaataaa aatacagttg attttcaaga taactatgat ggcagtgaac gtgaacctct
    10861 tgttttacca gcgcgtttcc ctaatttatt agtcaatggt gcaactggta ttgctgtagg
    10921 tatggcaaca aatattccac cacataatct aggtgagtct atagatgctg tcaagttggt
    10981 tatggataac cctgatgtta ctacacggga gttaatggaa gttattcctg gtccagattt
    11041 tcctactggt gccttggtga tgggacgttc aggtattcac cgtgcctatg aaactggaaa
    11101 gggatcaatt gtcttacgtt cacggactga aattgaaaca acttcaaatg ggaaagagcg
    11161 tattgttgtc acagagtttc catacggtgt taataaaaca aaagtacacg agcatattgt
    11221 acgtttagca caagaaaagc gtattgaagg tattactgct gttcgtgatg aatcaagtcg
    11281 tgaaggcgtt cgttttgtta ttgaagttcg tcgtgatgct tcagcaaatg tgattttaaa
    11341 taatcttttc aaattaacta gcttgcagac aaattttagc ttcaatatgc ttgctattga
    11401 aaaaggtgtt ccgaaaattt tgtcattacg acaaatcatt gataactata ttgaacacca
    11461 aaaggaagtt attgtccgtc gtactcagtt tgacaaagct aaagcagaag ctcgtgctca
    11521 tatcttagaa ggtttacttg tggcgcttga tcacttggat gaagttatca cgattattcg
    11581 taatagtgag acagacacca ttgctcaagc agagttgatg tcacgttttg aattatcgga
    11641 gcgtcagagt caagcaattt tagatatgcg tcttcgtcgt ttaactggat tagaacgcga
    11701 taaaatccaa tcagaataca atgacttgct agctttaatt gctgatttag cagatatctt
    11761 agctaagcct gaacgtgttg ttaccattat aaaagaagag atggatgagg ttaagcgtaa
    11821 gtatgctgat gcccgtcgta cagaattaat gattggtgaa gttttatcac ttgaagatga
    11881 agaccttatt gaggaagaag atgttcttat tactctctca aacaagggat atatcaagcg
    11941 tctcgctcaa gatgagttta gagcacaaaa acgtggagga cgcggtattc aagggactgg
    12001 agttaataac gatgattttg ttcgtgaatt ggtttcaaca agtactcacg atactgtgct
    12061 tttctttaca aatttagggc gtgtgtatcg tttgaaagca tatgaaattc cagaatatgg
    12121 acgtacagct aaaggcctcc ctatcgttaa ccttttaaaa cttgacgaag gtgaaacaat
    12181 tcaaacaatt attaatgcta gaaaagaaga tgtcgctaat aaatatttct ttttcacaac
    12241 tcagcaaggt atagtgaaac gtacaagtgt ttctgaattt agtaacattc gtcaaaatgg
    12301 acttcgtgct attaatttga aagagaatga cgagttgatt aacgtcctct tgattgacga
    12361 aaatgaagat gttattatcg ggacacgtac aggctactct gttcgcttca aagtgaacgc
    12421 tgtacgtaat atgggacgca cagcgactgg ggtacgaggc gttaatcttc gtgaaggtga
    12481 taaagttgtg ggagcttctc gtatcgttaa tggacaagaa gtactgatca tcactgaaaa
    12541 aggttatggc aagagaacag aagcttcgga atatcctaca aagggtcgtg gtggtaaagg
    12601 aataaaaacc gctaatatta ctgctaaaaa tgggccatta gcacgcttag tgacgattaa
    12661 tggaaatgag gacatcatgg tcattacaga tacaggcgtt attattcgaa ccaatgttgc
    12721 taatatttct caaactggtc gttctactat gggtgttaaa gtgatgagac ttgatcaaga
    12781 agctaaaatc gttactgttg cgctagtgga acaagaaatt gaagataagt ctaatataga
    12841 ggatacaaaa gaataggaag ttatgcgtaa taaaaaaaaa ttacatggtt tctttaactt
    12901 tgttagatgg ttattggttg tattgctaat tattgttggg ttggctttgg tgttcaataa
    12961 accaattcgc aatgctttca tagctcatca atcaaatcat tatcaaattt cacgagttag
    13021 taaaaaaaca attgaaaaaa ataagaaatc taaaacaagt tatgactttt catcagtcaa
    13081 atctattagt acagaatcga ttttatctgc tcaaacgaaa tcacataatt tgcctgtaat
    13141 aggagggatt gctataccag atgtcgaaat caatctccca attttcaaag gactaggcaa
    13201 tacagagttg agttatggtg caggaacgat gaaggaaaac cagattatgg gaggccaaaa
    13261 taactatgct ttagctagtc accatgtctt tggtttgaca ggttcttcta agatgctctt
    13321 ttcgccttta gaacatgcta agaaaggtat gaaagtttac ctcactgata aaagcaaagt
    13381 atacacttac actattactg aaatttcaaa ggtaacacca gaacatgttg aggttattga
    13441 tgatacacct ggcaagagcc aactcacttt agtgacctgt acggatccag aagccacaga
    13501 acgtattatt gtgcatgctg aattagaaaa aacaggtgaa ttttcaacag cagatgagtc
    13561 gatattaaaa gcattttcga aaaaatataa tcaaattaat ctctagaagg tagttagaat
    13621 tatgaaatta aaggctgttc atcatattgc gataattgtt tctgactatg aaaagtcaaa
    13681 agacttctat gttaataagc taggttttga aattattcgt gaaaaccatc gtccggagcg
    13741 tcatgattat aaattagatt tacggtgcgg tgatattgag cttgaaatat ttggaaatcg
    13801 tcttgatgat ccagaatatg aaacccctcc tcagagaata ggtaggccga attggccacg
    13861 tgaagcatgt ggtttacgac accttgcctt ttacgttcca gatgtggaag cgtacaagat
    13921 agaactggag aatttaggaa tttttgtaga gcctattcgt tacgatgact atactggcaa
    13981 aaagatgacc tttttctttg atccagatgg tttaccttta gaattacatg aataataaac
    14041 aaagttgcgt ggatacgcaa ctttgtttta atttactgaa taattttata ttcaagaagt
    14101 tttttaaaga aataagtaag ttaatcatgg tataatgtaa ggtatgaaaa gaattgaaaa
    14161 ggaattattt tgaaatgagg atactaaaat gagtattaaa aaactattgt ttgcaggcct
    14221 tgcttttatt ttatttaccc tagcgagtcc agcatatgct gcatcagatg ttcagaaagt
    14281 tattgatgaa acttatgttc aacctgatta tgtcttaggt tattcgctaa atcaagaaca
    14341 gcgtgcacag acattacaac ttctgaatta tgatgaatct agagacacga aagttaaaac
    14401 gctaaatact agctcatatg ctaaaataat gaatatagct gatgatgcaa gcatccaatt
    14461 atattcatct gttaaaataa aaaaattagg gtctaacgat accttagcag taaacattgt
    14521 tacacctgag aatatcacta aagttacaga agatatgtat cgcaatgctg cagttacatt
    14581 aggtattgag catgcaacga tatcagtggc tgctccaata aaagtaactg gggagagtgc
    14641 tcttgcaggt atctattatt cattagagaa aaatggagca agtgtatcat cagaaaataa
    14701 acaactagca caagaagagt taagtacttt atctggtatt aatgctgaga ataagggtaa
    14761 ggaaggttat gacgccgaca agcttaatgt agctttaacg gatattaagt cagccgtagc
    14821 taaaggagga tctgatcttt caaaagatga tattcgaaaa attgttgagg aaacattgaa
    14881 aaattatcat cttgataacg ctgtaacgga aaatcaaata aatttaattg ttaattttgc
    14941 agttaatctt tcacaaagta atgtcatcaa aaatagtgat ttcacgaaca ccttgaataa
    15001 tttgaaagac aacattgttt cgaaagcagg atctaaattt aaaaatatca atgtcaattt
    15061 taatgctaat aaagctgttg aatcaggtaa gggattttta gctaatattt ggcaacaaat
    15121 tgttaatttt ttccagcagt ttggtagtta ataaaaaagt ctagcttata agctagactt
    15181 tttttaccct aaataaatgt ttgtgttttt ttaactaatc tttaaatgaa tatttaagaa
    15241 taaatggcgc aataatagtt gatagtataa caacaataac aagctcggaa taagtagtgc
    15301 ttgaaataat tttagcagct aatccaactt gaactataat gagagccatc tcgccacgac
    15361 tgaccattcc accaccaatt gttaaagatt ctaattttga aaaattaaag cctcgaccga
    15421 caaagtaccc tggaattaat tttgataaaa cagccaaagc agtaaagatt aatatcgtgt
    15481 gaaggtgaga catcattcca tcgaatttaa gaggtagtgc aattgaagcg aagaaaattg
    15541 ggataaagaa ggtgtaacta agaagtgaaa ttttatgttc taccttatca acaaaagaag
    15601 tttgtccaat agctaatcct gcaaaaaacg aaccaatgat agaactcatt ccaactttat
    15661 cagctaaaat tgatagagaa aaacagataa ggatagcaag aatagtgtat ttctcaaaga
    15721 aatgaacctt ttcgattaat ttgaaaagag cgggtacaag gtatttcata caaataaata
    15781 agaaaacaaa gaataacaat tgaatgataa tttgtaatcc gatattagaa gagcttccat
    15841 ttgttgcaat aaagacactt agaagcaaaa cagctaagac atcatcagca acagcagcac
    15901 ctaaaataat agcaccagta tctgtcttta cacgattgta ttcttgtaaa acttcaactg
    15961 tgatagaaat actagttgca gcaaatacga gcccgtaaaa gatagcagta ttgatttgga
    16021 acccaaacaa gcgtgtcaag aagtagaaaa gagccatagg aacaataaca ccagtaattg
    16081 cgactagtag actgggctta agatacttct ttaaaagatc aaaattagct tcaagacccg
    16141 ctaaaaacat gagtaagata acaccaattt ctgacaggac gtgtaatacc tgattttgat
    16201 gaacaagtcc tagcattgca ggtccgatga tgatacctac aagcagttgt ccaacaacag
    16261 ccggtattcc aattcttttt gaaataagag ttgcaagaac gcttgccaac aaaatgattg
    16321 taatttgaat gataatatgc attttttcct cctaatatta ataaaatgat atcaatcata
    16381 ttgtactgaa tatttagaat accatttttt aagcctaaaa tctaggaata tttcttttct
    16441 gcctttttca tattattatc aagaaaatgg aaaaatatac tgaaaaatat tcattttaag
    16501 aataattaac ttgtaactat cgaactatat aaaaatgata ttttaagtgt aagtatccat
    16561 tattcaagaa ataagagtaa aaaaacttag ctacttaaag tagctaagtt ttta
//
LOCUS       DATRTN010000035        16385 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.035, whole genome shotgun sequence.
ACCESSION   DATRTN010000035 DATRTN010000000
VERSION     DATRTN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16385
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.035"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            45..1322
                     /locus_tag="VBE22_001132"
     CDS             45..1322
                     /locus_tag="VBE22_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEO7202599.1"
                     /translation="MKKMGYYMGLAAACLALLAGCSNNSNKESKASDNKSGTTEISYA
                     IWDSAQEPGIRKIADKFEEKNPKIKVKIQVVSWDAYWTMLEAGATGGSLPDTFWMHSN
                     EIYRYGANKMLLSLDDYIKKSKEVKLSNYPKGLTKIYNIKGEQYAIPKDYDTIGLWYN
                     KKMFDAAGVSYPDETWDWNKLKEAAKKLTKPDGSQYGFLAPLHNQEGYYNFVYQNGGT
                     IITKDKKSGYDDPKTVEALKYYLSFVKENLSPKVTEDKKRAEVMQNGQAAMGYFGSWN
                     LNSFASNDYMKENFDVAVLPKANDGKRSTIFNGLGNAISAKTKHPQAAWKWVEYLSSK
                     EAQDMQAKLGVAISAYKGSDKVWVDSNKTFAIKHFVEMIDYAQIRPYSNATTKWEDKV
                     YEELKPAYLGKESLEKAVKKITKTMNRALEREK"
     gene            1407..2297
                     /locus_tag="VBE22_001133"
     CDS             1407..2297
                     /locus_tag="VBE22_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7202600.1"
                     /translation="MKNKKKWNEYLWGWALVAPTIIGLMVLNIIPIFQTLKMSFFKSG
                     DFGQNDIFVGLDNYKRLIEDEQVWQATWNTFKYTLLVVPLTVILALVLATLLNSKIKG
                     KHVYRTIFFLPMVAAPAAVTMVWKWLYNTDFGLINFLLRKIGLNGLNWIEDPNIALYS
                     IALIGIWSTVGYSMILILAGLQEIPKDFYESATIDGASAVKQFFSITLPLISPTLFFV
                     VVTSIIQAMQVFDAIYLMESVTSPAYNKTVSLVYLFYNNSFKYADQGYGSTIVMLLLV
                     IILIITLIQMKVQKKWVYYS"
     gene            2311..3144
                     /locus_tag="VBE22_001134"
     CDS             2311..3144
                     /locus_tag="VBE22_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO7202601.1"
                     /translation="MYKTKKKFPYLWAHVFLIFVALGMIIPFIWMVLTSFKTITESTQ
                     LNPFHFFPSKWQVSNYTEVIRTNNFPILYFNTIMMMLWRIVSSVLFSAMAAYAFARLK
                     FPGRNLLFGLVLFQMMVPPQLFVIPQYLMVNQLNMRNTIFALVFPGLVSAFGTFLLRQ
                     FFIGLPDELEEAAKLDGCNIGQTFFNIMLPLAKPGLIALGIFTALFAFKDLLWPLIIN
                     SESDAATLSSALSKIQGAYAVNYPQLMAASVLAIWPMVVLYIIFQKQFIQGISTSGGK
                     L"
     gene            3154..5355
                     /locus_tag="VBE22_001135"
     CDS             3154..5355
                     /locus_tag="VBE22_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEO7202602.1"
                     /translation="MPINFREKEKEFHLYNDSLSYVICVLPNGHIGNLYYGKRLSENM
                     SYAYLLENKYRPLTAYVYEDDDCFSLQHTRQEYASYGSGDMILPAFEISHEDGSHLSH
                     FVFYDYAISKGKPQLSGLPNTYVESDEEATSLELYLKDHNSETELCLTYTIYESRAVI
                     TRNAKFTQRGEKELCLKRALSLCLDLPDKDYEWLHLDGAWGRERYLQSSLLHQGSQSI
                     YSLKGASSAEHNPFIALKRPDANEHEGEVMGFSLVYSGNFLAQVDVTPFHQTRVSMGI
                     HPLGFEWYLKSGESFQTPEVVMVYSHLGLNGMSQIYHDLFSQRLARGEWRDRERPILL
                     NNWEAMTFNFNENSILSLAEVAAKLGVELFVMDDGWFGQRNNEKAGLGDWRVNQKKLP
                     NGLLGISQRIHQLGMLFGLWIEPEMVNKDSDLYREHPDWILHHPKHSQSQGRHQYTLD
                     LSREEVYQNIYQQLHDLLANHGIDYIKWDMNRYMTEAYSYHATKEHQGAIFHRYILNL
                     YRLYETLTSEFPHILFESCSSGGARFDPGMLYYAPQTWTSDDTDAYERLKIQYGTSLV
                     YPLITMGNHVSEVPNQQLLRTTPLSTRANVAYFGSFGYEIDLMLLEEEELELIKKEID
                     FYKNYRRVFQFGNFTRLINPFETDDAAWQVISEDGHHVFVGLYLGLLKLNGPSYHRLH
                     LKGLEAEASYRLNNQVYLGSALMNAGLVINEKDFTEGYKDFASAIYHLEKM"
     gene            5445..6617
                     /locus_tag="VBE22_001136"
     CDS             5445..6617
                     /locus_tag="VBE22_001136"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEO7202603.1"
                     /translation="MTEKEIQTAFANVFHNEAESFFFSPGRINLIGEHTDYNGGHVFP
                     AAITLGTYGAARKRNDQLLRFYSSNFEDIGVIEVNLNNLVYNKADNWANYAKGVLKFL
                     QEAGHTIDTGMDIYVSGNIPNGSGLSSSASLELLIGIMAEEIFNLKLDRLDLVKIGKQ
                     TENKFIGVNSGIMDQFAIGMGADKQAIYLDTNSLAYDLVPLELGDNIVVIMNTNKRRE
                     LADSKYNERRAECEKAVEELQIALDIKTLGDLDEATFDEYSYLIKDANRLKRARHAVS
                     ENQRTLKAKEALISGDLLRFGRLINASHVSLEHDYEVTGIELDTLVHTAWEQDGVLGA
                     RMTGAGFGGCAIAIVAKDNVKQFTANVGKIYSEIIGYSPSFYIAEIAGGSHILTCK"
     gene            6627..8111
                     /gene="galT"
                     /locus_tag="VBE22_001137"
     CDS             6627..8111
                     /gene="galT"
                     /locus_tag="VBE22_001137"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEO7202604.1"
                     /translation="MMKTLLDKFVDQLIEIGTFEELDRIYLNNRIMALVGEEGLDQQT
                     DAESLIDIKDKLVDIAVKNGACQELLSKKDMLGAQLMDFITPLPSQVNAAFWKTYKEN
                     PKQAIADFYQLSKANDYIKVKANAKNIYYQVTTDYGNLEITINLSKPEKDPKAIAEAK
                     LSKECHYPKCQLCLENEGYQGRIDYPARTNHRVIRYEINKEIWGFQYSPYAYFNEHCI
                     FFYGKHQPMKISSKTFERLLDIVDRFPGYFAGSNADLPIVGGSILTHEHYQGGRHTFP
                     MEVAEIEEDLTFKGFKKVRAGIVKWPISVIRLRSENQQQLIALATKILENWQNYSDES
                     VHILSESSGIPHHTITPIARKKGNSFELDLALRDNQTSTEFPDGMYHPHPDVQHIKKE
                     NIGLIEVMGLAILPPRLKEELGLVERYLLGEVNEVASYHQKWADNLKLVSEITSGNVE
                     QIVKDAVGKVFSRVLEDAGVFKRTPEGKAAFRRFVESVGVIKGE"
     gene            8113..9108
                     /gene="galE"
                     /locus_tag="VBE22_001138"
     CDS             8113..9108
                     /gene="galE"
                     /locus_tag="VBE22_001138"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEO7202605.1"
                     /translation="MAILVLGGAGYIGSHMVDYLLTKTDETVVVVDNLVTGHREAIHL
                     EAIFYKGDLADKTFMRQVFVENPSIDTVIHFAAYSLVAESMEQPLKYFNNNTAGMINL
                     LEVMTEFAIKFIVFSSTAATYGIPNDIPIKEITPQRPINPYGESKLMMETIMKWADAA
                     YGVKFVPLRYFNVAGAKPDGSIGEDHKPETHLLPIILQVAQGKREKVMIYGDDYQTSD
                     GTNIRDYVHPFDIAAAHLLAVDYLRKGNPSTAFNLGSSIGFSNFQILEVARRVTGKDI
                     PVEVTKRRIGDPDILIADSSKARDILGWQPEFDDIEKIISSAWAWHSSHPDGYKS"
     gene            9234..10235
                     /locus_tag="VBE22_001139"
     CDS             9234..10235
                     /locus_tag="VBE22_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEO7202606.1"
                     /translation="MTIIKKNFGEKLAFLYELKNKNGMKVTLTNFGARIVDIFIPIDE
                     HLRNISLSATSVEDYYQKDIYVGATVAPVAGRISGAKTHIKGKCYHFTENEPNRTLHS
                     GVDTSVEQYWSTEIEDKENKIIFSLFLPDGKNGFPGNVTVIATYQLTEENELKVNYQA
                     QSDCDTIFNPTNHVYFNLTGDFQKSVASHQLKISSQEFVPLGKDHLPTGEILTVEGTD
                     FDFREFALFSKGLNRFSFQTRLVNGFDHPWLLEVGNNAVEVVSPDNKISLIMKTNQPA
                     VVIYTYNHGPTALAACHGAFSLECQSLPNACNLENLGSILLEKGKVFDSHFSYRFVF"
     gene            10426..10680
                     /locus_tag="VBE22_001140"
     CDS             10426..10680
                     /locus_tag="VBE22_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEO7202607.1"
                     /translation="MSYEQEFLKDFEEWLQSQISINQMAMDSAKKVLEEDRDERAADA
                     YIRYESKLDAYRFLQGKFNNYHNQKSFHDLPDGLFGQRHY"
     gene            10789..11100
                     /locus_tag="VBE22_001141"
     CDS             10789..11100
                     /locus_tag="VBE22_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202608.1"
                     /translation="MAKRIKPNKKRELLKAKGINWKDVRLSQTLQEVASPKKKAPEAP
                     KAVDLLEGLTANKQDVLKEAGLVSLEAFAKVSEADVLALKGIGPAAIKQLVDNGVVFA
                     K"
     gene            11368..11946
                     /locus_tag="VBE22_001142"
     CDS             11368..11946
                     /locus_tag="VBE22_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7202609.1"
                     /translation="MQILEDFDGKALPKLETDRLILRQRTVGDVPAMFDYVCLEEVAY
                     PAGLSPIASLEDEYDYFENRYYQNLEKAKLPSGYGITVKGSDRIIGSCDFNHRHEDDV
                     FEIGYMLHPDYWGHGYMTEAVAALIEVGFTLLNLHKIEIRCYDYNKQSQRVAEKLGFT
                     LEATIRDRKDNQGNRCVNLIYGLLRSEWEGRK"
     gene            11943..12635
                     /locus_tag="VBE22_001143"
     CDS             11943..12635
                     /locus_tag="VBE22_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202610.1"
                     /translation="MNDFFNWLITLYNDDKVAFVSLCLTLVFGFFSWLAQLKRNKQDD
                     IRQAEQDEFNRLAQDELKEFQSFQKKFAEYQQKVSELQFNFESQENFKPYFEFSKTKS
                     STYVEEGKLIARICLTNVGRGTATKITTNPIDYDQDGNPMYFRTIPTKQTHAIYDYFS
                     ETFAIPNDSIFLSTTALEQTENQNHCIEFKIKFCDSSGRTYEQKFEFGYDNFLVRGIN
                     LKSVTYEPTLIK"
     gene            12657..15308
                     /locus_tag="VBE22_001144"
     CDS             12657..15308
                     /locus_tag="VBE22_001144"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEO7202611.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     ISRYDLGREKFLDKVWEWKDEYAATIKSQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYNRGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGQNVILPIINKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFAVGQRHNLPQVNVMNDDGTMNELADEFNGMDRFEARKAVVAKLESLGNLV
                     KIEKMTHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FMSWMENVHDWVISRQLWWGHQIPAWYNVNGEMYVGEDAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPDTEAADFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFSNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDQARENVEKVVNSQVGNVTDRWILHNLNETVGKI
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDEKVVTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFGQYAEGSIVLASYPQVNATFENQTAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILVKTSDSELESFFKDNSNYIKRFTNPETLEISSAIATPELAMSS
                     VITGAEIFLPLADLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDATQERIVEMQKLVK"
     gene            complement(15526..16305)
                     /locus_tag="VBE22_001145"
     CDS             complement(15526..16305)
                     /locus_tag="VBE22_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202612.1"
                     /translation="MLNSDFIISKSLANYIHHRRLEVGVSSTDLAEISNMSKSDWESF
                     EKNGGAIPLKSKDIILDLLFLERFPKEKECDFIDKLFEEAKENKLWPEKIYQTMGLTP
                     ALSFIAGCEILSDDINNDLEELSKLPKESHLGQLDTSLLLSLLPQQFITKYDYEFVYK
                     LSKVLAQYTSRNKVGSSYTAHNVIEEICLYLIAKESVLYFESLDENSHLQLKELLDYN
                     DEWPFDIFDDMDSYTFLYTDIYIEEDSPYHFKNWFVPQFYL"
BASE COUNT         5376 a         2674 c         3284 g         5051 t
ORIGIN      
        1 ataagctttt ctaaccaact atattaaata ataggagtgt agctatgaaa aaaatgggtt
       61 actatatggg attagcagcg gcttgccttg cactattagc tggatgttct aataacagta
      121 ataaagaatc caaagcgtct gataataaat caggaacaac agagatatca tatgctattt
      181 gggattctgc tcaagagcct ggaattagaa agatagctga taaatttgaa gaaaaaaatc
      241 ctaaaattaa ggtgaaaatt caagtggttt cttgggatgc ttattggaca atgcttgagg
      301 caggagcaac aggtggatca ttgccagata ctttctggat gcactctaat gaaatttatc
      361 gttatggtgc aaataagatg cttttgtcac ttgatgatta cattaaaaag agtaaagaag
      421 ttaagctcag caattatcca aaagggttaa ctaaaatcta taatatcaaa ggtgagcaat
      481 acgcaatacc aaaagattac gataccattg gtttgtggta taacaaaaaa atgtttgatg
      541 cagcgggggt ttcatatcca gatgagacat gggattggaa caaattaaaa gaagctgcta
      601 aaaaactaac taagcctgat ggtagtcaat acggcttttt agcaccgctt cacaatcaag
      661 aaggctacta taattttgtt tatcaaaatg gtggtaccat tattacaaag gacaaaaaat
      721 caggatacga tgaccctaaa acagtcgaag ccttgaaata ctatttaagt tttgttaaag
      781 agaatttatc accaaaggtg acagaggata aaaaacgtgc tgaagtgatg caaaatggac
      841 aagcagccat gggctacttc ggctcatgga acctcaattc ttttgcttca aatgattata
      901 tgaaagaaaa cttcgatgtt gcagttctac caaaagcaaa tgatggtaaa cgttcaacga
      961 ttttcaatgg tttgggtaat gctatttctg ccaaaacaaa acatccacaa gccgcttgga
     1021 aatgggttga atacttaagt tcaaaagaag cccaagatat gcaagccaaa cttggtgtgg
     1081 caatctctgc ttataagggt tcagacaagg tttgggtgga ttctaataag acttttgcta
     1141 taaaacattt cgttgaaatg attgattatg cacaaattcg accatattca aatgctacaa
     1201 ctaagtggga agataaagtc tatgaagaat taaaacctgc atacttaggc aaagaatcac
     1261 tagaaaaagc cgttaagaaa attactaaaa caatgaatcg agcgcttgag cgggaaaaat
     1321 aagatactga aaaaaactct ggagatggga tttaagggaa aatatttcca atctcgtctc
     1381 cagtttttaa ctaatgaggt acaaagatga aaaataagaa aaaatggaat gagtatttgt
     1441 gggggtgggc attggttgct cctacgatta ttggtttaat ggtcttaaac attattccta
     1501 tttttcaaac gttaaagatg agttttttta agagtggtga ttttggacag aatgatattt
     1561 ttgtaggact tgataattat aagaggttaa ttgaggatga acaagtttgg caagccacat
     1621 ggaatacttt taaatatact ctcttagtag ttcccttaac tgttattttg gcgctagttt
     1681 tagcaacgct tctcaactca aagattaaag gtaaacatgt ttatcgtaca atctttttcc
     1741 tacctatggt tgctgctcca gcagcggtca ctatggtttg gaaatggctt tataatacag
     1801 attttggctt aataaacttt cttttacgta aaataggatt aaatggtttg aattggattg
     1861 aggaccctaa tattgcccta tactccatcg ctttaattgg tatttggagc actgtaggtt
     1921 atagtatgat tttgatctta gctggcttac aagaaattcc aaaagatttt tacgaatccg
     1981 ctacgattga tggtgccagc gctgttaaac aatttttctc tataacgcta cctttaatct
     2041 cccctactct attctttgtg gtagttacta gtattattca agcaatgcaa gtttttgatg
     2101 ctatttacct aatggagtct gttacaagtc cggcctataa taagactgtt tcgttggtct
     2161 acctctttta caacaattct tttaaatatg ccgaccaagg ttacggatcc actatcgtaa
     2221 tgttattact tgttattatt ttgattatta ccttaatcca aatgaaggta cagaaaaaat
     2281 gggtatatta tagttagaaa gggtatcaat atgtacaaaa caaaaaagaa gtttccttat
     2341 ttgtgggcac atgtgttttt gatttttgta gctctaggaa tgatcattcc ttttatttgg
     2401 atggtcctaa cttcctttaa gaccattaca gaatcaacac aactaaatcc atttcatttt
     2461 tttccaagta aatggcaagt gtccaattac actgaagtaa tcagaacaaa caattttccc
     2521 attctttatt tcaatactat tatgatgatg ttatggcgta tcgttagttc agtattattt
     2581 agtgctatgg cagcatatgc atttgctcgt cttaaatttc ccggaagaaa ccttttattt
     2641 ggattagtcc tatttcaaat gatggttcct ccacaattat ttgttatccc acaatatcta
     2701 atggttaatc aactaaatat gcgaaatacg attttcgcgt tggtgtttcc ggggttggtt
     2761 agtgcctttg gtaccttctt acttcgtcag ttctttatcg ggctaccaga tgagttagaa
     2821 gaggctgcta aactagatgg ttgtaatatc gggcagactt tctttaacat tatgcttcct
     2881 ctagcaaaac caggtttaat cgctttagga attttcacag ctctatttgc ctttaaagac
     2941 cttttgtggc ctttaattat caattctgaa tcagatgcag ctactctttc aagtgcatta
     3001 tcaaaaatac aaggagctta tgctgttaat tatccacaac taatggcagc aagtgtactt
     3061 gctatatggc caatggtagt tctttatatt atatttcaga aacagtttat ccaaggaatt
     3121 tcaacttccg gtgggaagtt ataggagaga agtatgccaa ttaattttcg tgaaaaagaa
     3181 aaagaatttc atctctataa tgacagcctc agttatgtta tttgtgtctt gccaaatggg
     3241 catattggaa atctttatta tgggaaacga ttatcagaaa atatgtccta tgcttattta
     3301 ttagaaaata aatatcgacc acttacagcc tatgtctatg aagatgatga ttgtttttcg
     3361 ttacaacata cgcgtcaaga atatgcttct tatggcagtg gagatatgat tttaccagct
     3421 tttgaaatta gtcatgaaga tggtagtcat ttatcacatt ttgtttttta tgattatgct
     3481 atttcaaagg ggaagcctca attatctggt ttacctaata cctatgtaga atctgatgag
     3541 gaagcaacta gtctggagct ttatttaaag gatcataata gtgaaacaga actttgttta
     3601 acttatacca tctatgagag tagagctgtt atcacaagaa atgctaaatt tacccaaaga
     3661 ggcgaaaaag agctttgttt aaaaagagcc cttagccttt gcctagactt acctgataag
     3721 gattatgaat ggcttcattt agatggtgct tggggaaggg aacgttacct acaaagttcg
     3781 ctattacatc aagggagcca atcaatttac agtttaaagg gtgctagtag tgccgaacat
     3841 aatcctttta tagctttaaa aagacccgat gcaaatgagc atgagggaga agtaatgggc
     3901 ttttccttag tatatagtgg aaatttttta gcacaggttg atgtgacacc atttcatcaa
     3961 actagagtta gtatggggat tcatccgtta ggatttgaat ggtatttaaa gtcaggagaa
     4021 agtttccaaa cccctgaggt tgttatggtt tacagtcatt taggattgaa tggcatgagt
     4081 caaatctatc acgacttatt tagccaacgt ttggctagag gagaatggcg tgatagagaa
     4141 cggcctattc ttcttaataa ttgggaagct atgacattta attttaatga aaattccatt
     4201 ttaagcttag ctgaagtagc tgctaagctt ggtgtagaac tttttgttat ggatgatggc
     4261 tggtttggtc aaaggaataa tgagaaggct ggccttggag attggcgtgt gaatcaaaaa
     4321 aaacttccca atggtttact tggtattagt cagagaatcc atcagttagg gatgttattt
     4381 ggtttgtgga ttgaaccaga gatggtcaac aaagatagcg atctctaccg agaacatcct
     4441 gattggattt tacatcatcc taagcattca caatctcaag gacgccacca gtatacacta
     4501 gatttgtctc gggaagaggt atatcaaaat atttatcaac agttacatga tctgttagct
     4561 aatcatggta ttgactatat aaaatgggac atgaatcgtt atatgacgga ggcttacagc
     4621 tatcatgcga ctaaagagca tcaaggagca atctttcatc ggtatatcct aaacctttac
     4681 cgactatatg agacgttaac cagtgagttt ccacatattc tatttgagtc atgctctagt
     4741 ggtggagcaa ggtttgatcc aggaatgctt tattatgctc cgcaaacatg gactagcgat
     4801 gatacggatg cttatgaacg tctaaaaatt cagtatggta caagtctggt ttatcctctt
     4861 ataactatgg gaaatcatgt ttcggaggtt ccaaatcaac aactcttacg taccacaccg
     4921 ctttctacaa gagctaatgt agcttatttt ggaagttttg gatatgagat tgacttaatg
     4981 cttcttgaag aagaggaatt agagctaatc aaaaaagaga ttgattttta caaaaattat
     5041 cggagagtct tccaatttgg taattttacg cgtctaatta atccgtttga aactgatgat
     5101 gctgcttggc aagtcatctc agaagatgga catcatgttt ttgtaggtct ctatttaggt
     5161 ttgctaaaac taaatggtcc ttcttatcat cgtttacact taaaaggttt agaagcagaa
     5221 gcaagttatc gtttaaataa tcaagtctat ctaggaagtg ccttgatgaa tgctggctta
     5281 gttatcaacg aaaaagactt cacagagggc tataaagatt ttgcatcagc tatataccat
     5341 ttagaaaaga tgtaaaaagc tcgaactaac tttcacaaca cacttgttag tgctttgtta
     5401 gtagctaaca atagtaaatg taatagaagg agtgaaaaat aattatgact gaaaaagaaa
     5461 tacagacagc ttttgcaaat gtttttcata atgaggcaga gagttttttc ttctcaccag
     5521 gacgtatcaa tttaattgga gaacatactg attataatgg agggcatgtc tttccagctg
     5581 ctataacatt aggaacatac ggagctgcca ggaaaagaaa tgatcaatta ttacgctttt
     5641 actcaagtaa ctttgaagac attggtgtga ttgaagtcaa tttgaataat cttgtttaca
     5701 ataaagctga taattgggct aattatgcta aaggtgttct taagttttta caagaagcag
     5761 gacatactat cgataccggg atggatattt acgtttctgg caatatccct aacggttcag
     5821 gtttatcttc atctgcttcg cttgaattat tgattggcat tatggcagaa gagattttta
     5881 atcttaaatt ggatcgtctt gatttagtaa aaatcggcaa gcagacagaa aataaattta
     5941 ttggtgttaa ttctggtatc atggatcagt ttgctattgg aatgggagct gataaacagg
     6001 ccatctatct tgataccaac agtttagcat acgatttagt tccgcttgaa ctgggagaca
     6061 atattgtagt tatcatgaat accaataaac gtagagaatt agcagattct aagtataatg
     6121 agcgccgagc agaatgtgaa aaagcagtgg aagaattaca gattgctctt gacattaaaa
     6181 ctttgggaga cttagatgaa gcgacatttg atgagtattc ttatttgata aaagatgcta
     6241 atcgtctcaa acgagcccgg catgcggtat ctgaaaatca acgcacttta aaggcaaaag
     6301 aagctcttat atcaggagat ttattacgat ttggacgact aatcaatgct tctcatgttt
     6361 cgttagaaca tgactatgaa gtgactggaa tagagttgga cacactagtt catactgcgt
     6421 gggagcaaga tggcgtgctt ggagctcgaa tgactggagc aggttttggc ggttgtgcta
     6481 ttgctatcgt agctaaagat aatgtaaaac aatttacagc aaatgtcggt aaaatctata
     6541 gtgaaattat tggttattca cctagttttt atattgcaga aattgccgga ggtagccata
     6601 ttcttacttg taaataagga aataatatga tgaaaacatt attggataaa tttgtagatc
     6661 agcttattga aatagggacc tttgaagaat tggataggat ttacctgaac aatcgtatta
     6721 tggctttagt aggtgaagaa ggcttggatc agcaaacaga tgcagagagt ttgattgaca
     6781 tcaaagataa acttgtagat atagccgtga aaaatggtgc ttgtcaggaa ctattatcta
     6841 agaaagacat gctcggtgca cagttgatgg attttataac tccattacca agtcaagtta
     6901 atgctgcttt ttggaagacc tataaagaaa accctaagca ggctattgct gatttttatc
     6961 agttgagtaa agcaaatgat tatatcaaag ttaaagctaa tgctaaaaac atctattatc
     7021 aagtgactac agattatggg aatttagaaa ttaccatcaa cctctcgaag cctgaaaaag
     7081 accctaaagc tattgcagaa gctaagcttt ctaaagaatg tcattaccca aagtgtcaac
     7141 tttgtttaga gaatgaaggt tatcaaggtc gtattgatta tcctgcacgt actaaccatc
     7201 gtgttattcg atatgagata aataaagaaa tctggggatt ccaatattca ccttacgctt
     7261 actttaatga gcactgtatt ttcttttatg gaaaacacca gcctatgaag attagttcta
     7321 agacatttga gcgtctctta gatattgtcg ataggtttcc tggatatttt gcaggatcaa
     7381 atgctgacct tcctattgtc ggtggctcta ttttaaccca tgagcattat caaggaggtc
     7441 gccacacatt tccaatggaa gtagcagaga tagaggaaga ccttactttc aaaggtttta
     7501 aaaaagtaag agcaggtatt gtaaagtggc caatatcagt tatacgcttg cgaagtgaaa
     7561 atcagcaaca attaatcgct ttagcaacaa agattctaga gaattggcag aattattcag
     7621 atgagtctgt tcatatttta tctgagagtt caggtattcc acaccataca attacaccta
     7681 ttgctcgaaa aaaaggtaat agtttcgagt tagatttggc cttacgtgat aatcaaacat
     7741 caacagaatt tccagatggt atgtatcatc cacaccctga tgttcaacat attaagaaag
     7801 aaaatattgg cttaatagaa gtcatgggat tagcgatttt gccgccgcgt ctcaaggaag
     7861 aacttggttt agtcgaaagg tatcttttag gggaagttaa cgaggttgcc tcctatcacc
     7921 aaaagtgggc agataattta aaattagtaa gtgagattac ctcaggaaat gttgaacaga
     7981 ttgtcaaaga tgctgtaggt aaagtgtttt cccgtgtact tgaggatgca ggagtattca
     8041 aacgaactcc agaaggtaaa gcggctttta ggagatttgt ggagagcgta ggagtcataa
     8101 agggagaata aaatggcaat tttagtctta ggtggtgcag gttatatcgg gtctcatatg
     8161 gtagattact tactgacaaa gacggatgag acagttgttg ttgtagataa tttagtgacc
     8221 ggacatagag aagcaataca cttagaagcg attttctaca agggagattt agcggataag
     8281 acttttatga gacaggtatt tgtagaaaat cctagtattg acactgtaat tcactttgca
     8341 gcctattctc tggtagcaga atctatggag cagcctttaa aatattttaa taataataca
     8401 gcaggcatga ttaatctctt agaagttatg actgaatttg ctataaaatt tatcgtattt
     8461 tcttcaactg cagcaactta tggtattcca aacgacatac caattaaaga aataacgcct
     8521 caacgtccca ttaacccata tggtgaaagt aagttgatga tggaaactat tatgaagtgg
     8581 gctgatgctg cttatggggt taaatttgtt cctttgcgct attttaatgt tgcaggagca
     8641 aaaccggatg gttcaattgg tgaagatcat aaacctgaga ctcacctttt accaatcatt
     8701 cttcaagtag cacaaggaaa acgcgagaag gttatgattt acggtgatga ttatcaaaca
     8761 tcagatggaa ccaatattcg tgactacgtt catccatttg atatagccgc tgctcactta
     8821 ctagcggttg attatcttcg taaaggcaat ccttcaactg cgtttaattt aggttcctct
     8881 ataggattct ctaatttcca aattttagaa gtagctcgca gagtgactgg taaagacatt
     8941 cctgttgaag tgacaaaacg tcgtattggg gatccagata tccttatagc cgattctagc
     9001 aaagcccgtg acattttggg ttggcagcca gagtttgatg atattgaaaa gataatctct
     9061 tcagcatggg cttggcattc gagtcaccca gatggctata aatcctaaag aataaagctt
     9121 taaccatatg ataagaaatt tacttcaagc agattagtat ttttggatta gaaagagtct
     9181 tattagcata atgaatttat caggtatcat tagtttgaaa gaagaaaaaa tatatgacta
     9241 ttattaagaa aaacttcgga gagaagctgg cattcctcta cgaattaaaa aataaaaatg
     9301 gaatgaaggt cactttgacg aattttggtg cacgtatagt tgatatcttc attccgattg
     9361 atgaacactt gcgtaatatt agtttatcgg ctacttctgt tgaagattat tatcaaaaag
     9421 atatttatgt gggggcaacg gtggcccctg tggcaggtag aatttctgga gctaaaaccc
     9481 atatcaaagg aaaatgttat cattttactg aaaatgaacc taataggaca ttacatagtg
     9541 gtgttgatac atctgtagaa cagtattggt ctacagaaat agaagataaa gagaataaaa
     9601 ttatcttttc tctctttctt ccggatggta aaaatggctt tcctgggaat gtgactgtga
     9661 tagctactta tcagttgaca gaagaaaatg aactcaaggt caactatcaa gcacaatctg
     9721 attgtgatac catttttaac ccaacaaatc atgtatattt taatttaaca ggggattttc
     9781 aaaaatccgt tgctagccat cagttaaaga tttcaagcca agagtttgtg ccactaggga
     9841 aagaccattt acctactggt gaaatactga ctgtggaagg tacagacttt gattttagag
     9901 aatttgccct tttttcaaaa ggtctaaatc gcttttcttt ccaaacgaga ttagtaaacg
     9961 gatttgatca tccgtggctt ttagaagtag gcaataatgc cgttgaggta gttagtcctg
    10021 ataataaaat aagcttaata atgaaaacca atcaaccagc tgttgttatt tacacctata
    10081 atcatggtcc aacagcacta gcagcttgtc atggagcttt tagtttagaa tgccaatctc
    10141 ttcctaatgc ttgtaatcta gaaaacttag gatcaatttt gctcgaaaaa gggaaggtat
    10201 ttgatagcca cttttcttat cgttttgtgt tttaatatga catctaaaag atgtgatgcc
    10261 aaaactagaa tggcatagag aaccatacat ttcaataggt cttccttaga taaaactagt
    10321 tgtttttata tctgtcagaa aaatcataat ttatttttga tataatgatt ttttgagtat
    10381 ttaatttata tgttataatg aaaaaaacta aaaaagagga atatcatgtc atacgaacaa
    10441 gaatttttaa aagactttga agagtggtta caatcgcaaa taagtatcaa ccaaatggca
    10501 atggacagtg caaagaaagt tctagaggaa gatagggatg agcgagcagc agatgcgtat
    10561 attcgttatg agagtaagtt agacgcttat cgctttttgc aagggaaatt taataactat
    10621 cataaccaaa aatctttcca tgatttgcct gatggtcttt ttggacaacg tcattattaa
    10681 aaggtttatc gctagaaagc ttgtcaaata ggcatttcta tgatacattg ttatagtagt
    10741 ttttagatac tatgatagat ttcataaatg aggagaaagc attatacaat ggcaaagcgt
    10801 attaaaccga ataaaaaaag agagcttttg aaagctaaag ggatcaattg gaaggatgtt
    10861 cgactatctc aaactttaca agaagtcgca tcaccgaaaa aaaaggctcc tgaagcgcca
    10921 aaggcagtcg atcttttaga aggtttaact gctaataaac aagatgtttt aaaagaagct
    10981 ggccttgtca gccttgaagc gtttgcgaaa gtaagtgaag cagatgtttt agcactaaaa
    11041 ggtattggtc cagcagctat taaacagtta gttgataatg gtgttgtttt tgcaaaataa
    11101 tttgatttac cttgcgaaag tagccttatt ttgctagaat aaattaactt agaggtgttt
    11161 tgaaatccca tttgtcacag gaagcggtca tactgagaaa tccgcacaaa tgtcaaagct
    11221 tgttgctaag tccaataatt gaaacaaaac gagttgcgga tgttatcatc cgaatttggg
    11281 tggtaccgcg gaaatcgtaa tacgaaattc gtccctgtca gaattgacag ggattttttg
    11341 tatttaggag tgaggagatt gaagtatatg cagattctag aagatttcga tggcaaagca
    11401 ttaccaaaac tagaaacgga tcggcttatc ttgcgtcagc gaacggtggg ggatgtccca
    11461 gccatgtttg attacgtttg cttggaagag gttgcctatc cagcaggact cagcccaata
    11521 gcttctttag aagatgagta cgattatttt gaaaatcgct actatcaaaa tctggaaaag
    11581 gcaaaattac catctggtta tggtattacc gttaaaggaa gtgaccggat tatcggctct
    11641 tgcgacttta accaccgcca tgaagacgat gttttcgaga ttggctacat gcttcatcct
    11701 gactactggg gtcatggcta tatgacagaa gctgtcgcag ccttgattga agtcggtttt
    11761 acccttctca atctccacaa aatcgaaatc cgctgctatg actacaataa gcaaagccag
    11821 cgcgtggcag aaaaactagg attcaccctt gaagcaacca tccgtgatcg taaggataat
    11881 caaggtaatc gctgtgtcaa tttgatatat ggtttgctga ggagtgagtg ggaggggaga
    11941 aaatgaatga tttttttaat tggttaatta cactatataa tgatgataaa gttgcttttg
    12001 tatcactttg tctaacttta gtgtttggat ttttttcatg gttagcccaa ttaaaaagaa
    12061 acaaacaaga tgatattcga caagctgaac aggatgaatt taacagacta gctcaagatg
    12121 agttaaaaga atttcagagt tttcaaaaaa agtttgcaga gtatcaacaa aaagtgagtg
    12181 aattgcagtt taattttgaa agtcaagaaa atttcaaacc ctattttgaa tttagcaaaa
    12241 ctaaatcaag tacttacgtt gaagaaggaa aattgattgc aagaatatgt ctgacaaatg
    12301 tagggagagg cacagctaca aagattacga ccaatcctat tgactacgac caagatggaa
    12361 atccaatgta ttttagaaca atacctacta aacagacaca tgctatatat gattatttta
    12421 gtgagacttt tgctattcca aatgattcta tatttttgag tacaactgca ctagaacaga
    12481 cagagaatca gaatcattgt atagagttta aaattaaatt ttgtgattca tcaggcagaa
    12541 cttatgaaca aaaatttgaa ttcggttatg ataatttttt agtaagggga ataaatctga
    12601 aatcagtaac ttatgagcca accttgatta aataagagaa ttagaaagga cactatatgt
    12661 caaaagaact atcaccaaaa tacaatcctg ccgaggttga ggcaggccgt tatcaaactt
    12721 ggcttgacca agatgttttc aagccatcag gggatgctga ggctaagcct tattctattg
    12781 tgattccacc accaaacgtt acgggtaaat tgcacttggg tcacgcttgg gatacaactc
    12841 tacaagatat cattattcgt caaaagcgta tgcagggctt tgatacactc tggcttccag
    12901 gtatggacca cgctggtata gccactcaag ctaaagttga ggagcgcctt cgtgagcaag
    12961 gcatttcacg ttatgatctt ggtcgtgaaa aattcctaga taaagtttgg gaatggaaag
    13021 acgagtatgc agcaactatt aaatctcagt gggggaaaat gggtctttcg gttgattata
    13081 gccgtgagcg ttttaccctt gatgagggac tttcaaaagc ggtaagaaaa gtctttgttg
    13141 atttgtacaa tagaggttgg atctatcgtg gggagttcat cattaattgg gatccagcag
    13201 ctcgtacagc cctttcagat attgaagtta ttcacaagga tgttgagggt gctttctatc
    13261 atatgaatta tatgctggaa gatggctcac gcgctctaga agtagcaaca actcgtcctg
    13321 agactatgtt tggagatgtg gctgtagcgg taaatccaga agatccacgt tacaaggatt
    13381 tgattggtca aaatgttatc cttcctatca tcaataaacc tattccaatc gtcgctgatg
    13441 aacacgctga tccagaattt ggaacaggtg tggttaagat tacgccggct cacgacccta
    13501 atgacttcgc tgtaggtcaa cgccacaact taccacaagt taacgttatg aacgatgatg
    13561 gtactatgaa tgaacttgcg gatgaattta atggtatgga tcgtttcgaa gctcgtaagg
    13621 cagtagtcgc taaattagaa tcgttaggaa acttagtaaa aattgaaaaa atgactcatt
    13681 ctgttggtca ttcagaacga acaggcgttg tggttgaacc acgtttatct actcaatggt
    13741 tcgtcaagat ggatcaattg gctaagaatg ccattgccaa ccaagataca gaagacaagg
    13801 tagagtttta tccaccacga tttaacgata cttttatgag ctggatggaa aatgtccatg
    13861 actgggttat ctctcgtcaa ttatggtggg gacaccaaat tcctgcatgg tacaacgtca
    13921 atggtgagat gtatgttgga gaagatgccc cagagggtga cggatggaca caggatgaag
    13981 acgttctaga cacatggttt agttctgctt tatggccgtt ttcaacaatg ggatggccag
    14041 acaccgaagc agctgacttc aaacgctact tcccaacgtc aaccttggta actggatacg
    14101 atatcatctt cttctgggta tcgcgcatga ttttccaatc ccttgaattt acaggacgcc
    14161 aaccattctc aaatgttctt attcatggac tcatccgtga tgaagaagga cgtaaaatgt
    14221 ctaaatcact tggcaatggg attgatccga tggatgtcat tgagaaatat ggtgcggatg
    14281 cccttcgttg gttcctctca aatggttcag cccctggtca agatgtgcgt ttttcatacg
    14341 aaaaaatgga tgcttcgtgg aatttcatta ataagatttg gaacatttca cgctatatcc
    14401 ttatgaataa cgaaggttta acgttggatc aagccagaga aaatgtggaa aaagttgtta
    14461 atagtcaagt tggaaatgta acagaccgtt ggattctcca caatttaaat gagactgtgg
    14521 gtaaaattac tgaaaacttt gacaagtttg aatttggtgt tgctggtcac atcctttaca
    14581 actttatctg ggaagagttt gctaattggt acgttgagtt aacaaaagag gttctttata
    14641 gtgacaatga agatgagaaa gttgttacac gttctgttct tctttatacc ttagatcaaa
    14701 tcttgcgtct tcttcatcca atcatgccat ttgtgacaga agaaattttt ggacaatatg
    14761 cagaaggatc aattgtcctt gcaagttatc cacaagttaa cgcgacattt gaaaatcaaa
    14821 cagctcacaa aggcgttgaa agcttgaaag atttgattcg ttccgtgcgt aatagcagag
    14881 cagaggtcaa tgttgctcca tctaaaccaa tcactatctt ggttaagaca agtgacagcg
    14941 aactcgaaag cttcttcaaa gacaatagca actacatcaa acgcttcaca aacccagaga
    15001 cgcttgaaat cagctcagca attgccacac cagaacttgc catgtcatca gtcatcacag
    15061 gagctgaaat cttcctccca cttgctgacc tcctcaacgt agaagaagag ttggcacgac
    15121 ttgaaaaaga acttgctaaa tggcaaaaag aactagatat ggttggcaaa aaactcagca
    15181 acgagcgctt tgtagccaac gctaaaccag aagttgtcca aaaagaaaaa gacaaacaag
    15241 ccgactacca agctaaatac gacgcaaccc aagaacggat tgtagagatg caaaaacttg
    15301 ttaaatagtt aaaatcatcc attacttccg cagagtcctg ccgctagacc atcagtctaa
    15361 gcgacagggc tttttgctat attcaaagat tgtgaaaaat taatatatat gatgcctact
    15421 tcttgcgtct gaaatacaag aagtgaaaat gatttggcca taaagaaaaa caggcagaaa
    15481 cccaaatgat taatgcctat ttcttatatt ttttcgtcaa ttattttata aataaaattg
    15541 aggtacaaac cagtttttaa aatgataagg tgaatcttct tcaatataga tgtctgtata
    15601 taaaaaggta tagctatcca tatcatcaaa aatatcaaaa ggccactcat cgttataatc
    15661 taataattcc ttaagttgta gatgggagtt ttcgtccaga gattcaaaat aaagaacgga
    15721 ttcttttgca attaaataga gacaaatttc ctctattaca ttatgcgctg tataagatga
    15781 accaacttta tttcttgatg tatattgagc caaaactttg cttaatttat aaacaaactc
    15841 ataatcatat tttgtgataa attgttgagg caatagtgat aaaagtaggg aagtatctaa
    15901 ttgtcccaga tgactttctt taggtagttt agataattcc tccaaatcat tattaatatc
    15961 atcagaaagt atttcacaac cagcaataaa tgaaagagca ggagtaagtc ccatagtctg
    16021 gtaaatcttt tcaggccaaa gtttattttc ctttgcttcc tcaaataatt tatctataaa
    16081 atcacattct ttctctttag ggaatctctc caaaaataat aagtcaagta tgatatcctt
    16141 agatttcaaa ggaattgccc caccattttt ttcaaatgat tcccaatctg atttagacat
    16201 attggatatt tctgccaaat cagttgacga cacgccaact tctaacctac gatggtgaat
    16261 gtagttagca agtgacttac taataataaa atcagaattt agcattccgt tctccttttg
    16321 attcctattc attatgatta tcacatataa aaaatttttt tcaagtaaaa acacatgcat
    16381 ctttt
//
LOCUS       DATRTN010000036        16373 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.036, whole genome shotgun sequence.
ACCESSION   DATRTN010000036 DATRTN010000000
VERSION     DATRTN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16373
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.036"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            153..1358
                     /locus_tag="VBE22_001146"
     CDS             153..1358
                     /locus_tag="VBE22_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEO7202613.1"
                     /translation="MSQKSNYFKGQIDYAVVIPVFFLLMIGLASIYVATMNDYPSNIY
                     IAMFQQVSWIIMGCIIAFVVMLFSTEFLWKATPYLYALGLTLMVLPLIFYSPQLFAAT
                     GAKNWVTIGSVTLFQPSEFMKISYILMLSRITVSFHQKNRKTFQDDWKLLGLFGLVTL
                     PVMILLMLQKDLGTALVFLAILSGLILLSGISWWIILPILSTIVLFIASFLMIFISPN
                     GKEWFYNLGMDTYQINRLSAWIDPFSFADSIAYQQTQGMVSIGSGGLTGKGFNILELS
                     VPVRESDMIFTVIAENFGFIGSAIVLGLYLIIIYRMLRITIESNNQFYTFISTGFIMM
                     IVFHVFENIGAAVGILPLTGIPLPFISQGGSSLLSNLIGIGLVLSMSYQNTVRQAKAN
                     GSHLKYRSI"
     gene            1477..2037
                     /locus_tag="VBE22_001147"
     CDS             1477..2037
                     /locus_tag="VBE22_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO7202614.1"
                     /translation="MNYHDYIWDLGGTLLDNYESSTRAFVETLKEFGYQADHDSVYQK
                     LKESTADAIAYFIPEEADFLKEYKANEAKVLETPILFQGAKELLAKIQRQGSRNFLVS
                     HRDNQVIVILEKTEIIDYFTEVVTADNGFSRKPSPESMLYLKEKYQIDNCLVIGDRDI
                     DKQAGESAGFDTLLVDGSKSLMEIIE"
     gene            2038..3990
                     /gene="gyrB"
                     /locus_tag="VBE22_001148"
     CDS             2038..3990
                     /gene="gyrB"
                     /locus_tag="VBE22_001148"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEO7202615.1"
                     /translation="MTEETKNMEQRAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFAGHIKVYIEPDNSITVVDDGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKNGKIHYQEYQRGVVV
                     NDLEIIGDTDLSGTTVHFTPDPEIFTETTVFDFDKLAKRIQELAFLNRGLRISISDKR
                     EGQEVEKEYHYEGGIGSYVEFINENKEVIFENPIYTDGELDGISVEVAMQYTTGYQET
                     VMSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFNRFLLENPQVAKKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNNAEMNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKVRYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKAYIQPGVNQEEE
                     LRQALDTYSSGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVSVDDAAEADKIF
                     DMLMGDRVEPRREFIEANAVYSNLDI"
     gene            4084..5808
                     /gene="ezrA"
                     /locus_tag="VBE22_001149"
     CDS             4084..5808
                     /gene="ezrA"
                     /locus_tag="VBE22_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEO7202616.1"
                     /translation="MSSGIILLLVAIVLLVIIAYVVGVVIRKRNDTLIANLETRKQEL
                     VDLPVQEEIEQVKLLHLIGQSQSTFREWNQKWTDLSTNSFKDIDFHLVEAENLNDSFN
                     FVRAKHEIDNVDSQLTIIEEDIVSIREALEVLKEQEEKNSARVTHALDLYETLQKSIS
                     EKEDNYGTTMPEIEKQLKNIEAEFSHFVTLNSTGDPIEASEVLNKAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKDIEQRFQEVREAIRSNSDGLV
                     SLDLDRARDENEHIQEKIDKLYDIFEREIAAYKVAHKDSKIIPQFLAHAKSNNEQLGH
                     EIKRLSAKYILNENESLSLRSFTNDLEEIETKVLPSVENFGQEASPYTHLQILFERTL
                     KTLTTVEENQMEVFEAVKTIESVETRARQNMDKYVNKLHMIKRFMEKRNLPGIPQDFL
                     STFFTTSSQIEALINELSRGRIDIEAVSRLNDVTTNAIANLEQATYLVVQDATLTEQL
                     LQYSNRYRSFEQNVQKSFEQALYLFEVEHNYKASFDEISYALETVEPGVTDRFVTSYE
                     KTQERIRF"
     gene            5902..6543
                     /gene="serB"
                     /locus_tag="VBE22_001150"
     CDS             5902..6543
                     /gene="serB"
                     /locus_tag="VBE22_001150"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEO7202617.1"
                     /translation="MIKGLLVMDVDSTLIMEEAIDLLAIEAGVGKQVAALTDAAMRGE
                     LDFEEALKKRVALLKGLPVTILTDILSSIHFTPGAYELIKECHKRQMKVGLVSGGFHE
                     TIDILAKQLQVDYVKANRLGVKGGFLTGEVEGEIVTKEVKKIKLKEWASENHLDLSQT
                     IAMGDGANDLPMIKSAGVGIAFCAKPIVREEAAYQINEKNLLKVFDIIDANEN"
     gene            complement(6564..7049)
                     /locus_tag="VBE22_001151"
     CDS             complement(6564..7049)
                     /locus_tag="VBE22_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO7202618.1"
                     /translation="MEIWDAYDQYGFITGLTLNRDQNIPQGLFHLVVDVILFHEDGDV
                     LMMKRHPKKKAFPAYFEATAGGSALKGENAKQAILRELKEETGIVPQCLTFLNREWFS
                     ERSYFVDHFIAKYNGAKDIITLQEGETVDYIWLKPEYIDLFLSKNKLIPSQIKLLKSL
                     I"
     gene            complement(7062..7517)
                     /locus_tag="VBE22_001152"
     CDS             complement(7062..7517)
                     /locus_tag="VBE22_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEO7202619.1"
                     /translation="MTELDNKILQKASGENRLDPDQQRYYLGTYKERVVLHLSLKEAQ
                     EEKFQLAITSALNNLILEYPNLSLKLSPNLPTDVQMFYMKLANRLSLAATIVNEANAN
                     SPFGIIIHSDKAENVEWNDFETQFPDLFNSPKKEESPKKSLWQHFFSQK"
     gene            7715..9022
                     /gene="eno"
                     /locus_tag="VBE22_001153"
     CDS             7715..9022
                     /gene="eno"
                     /locus_tag="VBE22_001153"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEO7202620.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYSGLGTQKAVDNVNNVIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEALRWGAEVFHALKKILKERGLETAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDAERKVYDYSKFEGEGGAVRTAAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTDYLARGIK
                     EEAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(9130..10194)
                     /locus_tag="VBE22_001154"
     CDS             complement(9130..10194)
                     /locus_tag="VBE22_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7202621.1"
                     /translation="MKRKQFIKLGIATLLTVISLYTPINLATNHTTENIVTAQEYKTK
                     ENGTLPFKHKRQLVLGELDDKGRATFAHIQLKVKDEPKKKRVKRLKTTPVGWHNFKFY
                     YNDGTQKAWLMSRGRLICHQFSGLNNERKNLVPMTNWLNTGNYNSTNSSNPESMLFYE
                     KQLKTWLSTHKNYYLDYKVTPIYQNNELIPRKIELKYVGIDKTGKLLPIFIGNKSTQD
                     QFGISTVTLENTSPNATIDYLSGKAQNTVLSAKEQRKLIAKHEEEKRLAEKKVEEEKA
                     AAETQKKLEEEQARLAAEAQRKQKEEQARLAAETQKKQETLVQEQTSQGYKRDYRGRW
                     HRPNGQYASKAEIAAAGLQW"
     gene            10423..11706
                     /gene="aroA"
                     /locus_tag="VBE22_001155"
     CDS             10423..11706
                     /gene="aroA"
                     /locus_tag="VBE22_001155"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEO7202622.1"
                     /translation="MKLLTNANTLKGTIRVPGDKSISHRAIIFGSISQGVTRIVDVLR
                     GEDVLSTIEAFKQMGVLIEDDGEIITIYGKGFAGLTQPNNLLDMGNSGTSMRLIAGVL
                     AGQEFEVTMVGDNSLSKRPMDRIALPLSKMGARISGVTNRDLPPLKLQGTKKLKPIFY
                     HLPVASAQVKSALIFAALQTKGESLIVEKEQTRNHTEDMIRQFGGHLDIKDKEIRLNG
                     GQSLVGQDIRVPGDISSAAFWIVAGLIIPNSHIILENVGINETRTGILDVVSKMGGKI
                     KLSSVDNQVKSATLTVDYSHLQATHISGAMIPRLIDELPIIALLATQAQGTTVIADAQ
                     ELKVKETDRIQVVVESLKQMGADITATADGMIIRGNTPLHAASLDCHGDHRIGMMIAI
                     AALLVKEGEVDLSGEEAINTSYPNFLEHLEGLVNA"
     gene            11699..12211
                     /locus_tag="VBE22_001156"
     CDS             11699..12211
                     /locus_tag="VBE22_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEO7202623.1"
                     /translation="MPKVLLGFMGVGKTSVANCLENEVIDMDSLIEKHIGMSISRFFT
                     EEGEASFRALESQFLNELLKKKNEGLVIASGGGIVLLEENRRLLTLNRHNNILLTGSF
                     EILYHRIKKDEKNRRPLFLNHSKEEFYDIYQKRMLLYSGLSDMIIDTDYLTPQKIATV
                     IGESLVNLSN"
     gene            12268..13641
                     /locus_tag="VBE22_001157"
     CDS             12268..13641
                     /locus_tag="VBE22_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO7202624.1"
                     /translation="MSRNNYGQLNHHEELRYNYLLKNIHYLNEREKMEFQYLHYKKTA
                     VRPQRRTESPPTNSYYEEPYSDSYYQDDDFYSEPQLTSQGLPIYQEERAPKKKKQRAR
                     KEKQRVKVMAPFPPKAITPPRKKKKFKGFLKFIGIILLIVLSGMVFMFVKGMRDVNNG
                     KSHYSPAIIEDFKGKDAVDGTNILILGSDKRISERSTDARTDTIMVANVGNKDNKVKM
                     VSFMRDLLVNIPNYSTEGYYDMKLNASFNLGEQDNHKGAEYVRQTLKNHFDIDIKYYV
                     MVDFETFADAIDTLFPNGVKINAKFGLVGGQSADSVKVPDDLRMKNGVVPSQKIKVGI
                     QYMDGRTLLNYARFRKDDDGDFGRTQRQQQVMRAIVSQIKDPRRLFTGSAAIGKAYAL
                     TSSNLSYSFVLTDGIPILSDAKNGIKQMTIPREGDWVDDYDQYGGQGLTIDFAKYKKI
                     LKKMGLR"
     gene            13742..15103
                     /gene="rlmD"
                     /locus_tag="VBE22_001158"
     CDS             13742..15103
                     /gene="rlmD"
                     /locus_tag="VBE22_001158"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO7202625.1"
                     /translation="MLHKNDIIETEISDISHEGMGIAKVDGFVFFVENALPGEIIQMR
                     VLKLRKRIGYGKVEEYLTTSPHRNEGLDYTYLRTGIADLGHLTYEQQLLFKQKQVADN
                     LYKIAHISDVLVEPTLGMTIPLAYRNKAQVPVRRVDGQLETGFFRKNSHTLVSIEDYL
                     IQEKEIDALINFTRDLLRKFDVKPYDEEQQSGLIRNLVVRRGHYTGQLMLVLVTTRPK
                     IFRIDQMIEKLVSAFPSVVSIMQNINDRNSNVIFGKEFRTLYGSDTIEDQMLGNTYAI
                     SAQSFYQVNTEMAEKLYQKAIDFSDLNSEDIVIDAYSGIGTIGLSVAKQVKHVYGVEV
                     VEKAVSDAKENATRNGITNSTYVADSAENAMAKWLKEGIKPTVIMVDPPRKGLTESFV
                     YSAAQTKADKITYISCNSATMARDIKLFEELGYHLVKIQPVDLFPMTHHVECVALLQR
                     SKG"
     gene            16201..>16373
                     /locus_tag="VBE22_001159"
     CDS             16201..>16373
                     /locus_tag="VBE22_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202626.1"
                     /translation="MHVGKSYFDYQENALIPQLGFLYSKLTKKIELDNRLSPTEQKLL
                     ITLLLHTKGLIID"
BASE COUNT         5343 a         2525 c         3250 g         5255 t
ORIGIN      
        1 tttcttttca tgttctttgt tttactgatg aagaacaatc tcctaatatt tgcaactcat
       61 aatgatttga gttgcatttt ttattgcaaa aaatataagt cagattctga aaaaatgtta
      121 taataattac catgaataaa aaaggaagtc ctatgtcaca aaaatcaaac tattttaaag
      181 ggcaaattga ttatgctgtt gtgataccag tatttttttt attaatgatt ggtttagcct
      241 caatttatgt tgcaacgatg aatgattatc cttctaatat ctatatcgcg atgtttcagc
      301 aggtgtcttg gattattatg gggtgtatta ttgcatttgt cgttatgctt ttcagtactg
      361 aatttttatg gaaagccacg ccctatctgt atgctttagg actaacccta atggttcttc
      421 ccttaatttt ttatagtcca cagctttttg cggcaacagg tgctaaaaat tgggtgacca
      481 ttggctcggt aactttgttt caaccgtcag agtttatgaa gatttcctat attttaatgt
      541 tatctcgaat aactgttagt tttcatcaga aaaatcgcaa aacttttcaa gatgattgga
      601 agttgctagg attgtttggt ttggttacat tgccagtgat gattcttcta atgttacaaa
      661 aagatttagg aacagcatta gttttccttg ccattttgtc aggtttaatc cttttatcgg
      721 gcatatcttg gtggataatc ttgccaattt tatctactat cgttcttttt atagctagct
      781 ttttgatgat ttttatctca ccaaacggca aggaatggtt ttataatttg gggatggata
      841 catatcaaat caaccgttta tctgcttgga ttgatccatt tagttttgca gactcaattg
      901 cttatcaaca aacacagggt atggtatcaa ttggaagtgg cgggttaaca ggtaaaggtt
      961 ttaatatttt agagctctcg gttcctgttc gtgaaagtga catgattttt actgtcattg
     1021 ctgagaattt tggcttcatt ggttcggcga ttgttttagg gttatatctc attatcattt
     1081 accgtatgtt aaggatcacc attgaatcta ataatcaatt ttatactttt atatcaactg
     1141 gttttatcat gatgattgtt ttccatgttt ttgaaaatat tggtgcagca gttggtatat
     1201 tacctctaac aggaatccca cttccgttta tttcgcaagg tggaagttca ttactaagta
     1261 atcttattgg aattggattg gtcttatcca tgtcttatca aaatacagtt cgtcaggcca
     1321 aggctaatgg atcacatttg aagtatcgta gtatataaca aaaaaaggtt ggaaagataa
     1381 tccagccttt taataatcga aatattttag aggagtgagt taattaagta aggtggtagc
     1441 tataaaaaat cgtaataata tgatataata aagggtatga attatcatga ttatatttgg
     1501 gatttgggtg gtacactact tgataattac gaaagttcca cacgtgcttt tgtagagact
     1561 ttgaaagaat ttggatatca agcagaccac gatagtgtgt atcaaaaatt aaaagagtca
     1621 acagcagatg caattgcata ttttatccct gaagaagctg attttttaaa agagtataag
     1681 gctaatgaag ctaaggtttt ggaaacacca attctattcc aaggggcaaa agaactttta
     1741 gcgaaaattc aaagacaagg tagtcgtaat tttttggtgt ctcatcgtga taatcaagtt
     1801 atagttattc tggaaaaaac agaaattatt gactatttta cagaagtagt gactgctgat
     1861 aacggctttt caaggaaacc tagtcccgag agtatgcttt atttgaaaga aaaataccaa
     1921 attgataatt gtttagtaat aggcgatcgt gatatagata agcaagcagg agaatcggct
     1981 ggttttgata ccttattagt cgatgggtca aaatcattga tggagataat agagtaaatg
     2041 acagaagaaa caaagaatat ggaacaacgt gctcaagaat atgacgcaag tcagattcaa
     2101 gtattagaag gtttagaagc tgttcgcatg cgtccaggca tgtatatcgg ttcgacttcg
     2161 aaagaagggc ttcatcacct tgtatgggaa attgttgata actcaattga tgaagcctta
     2221 gctggatttg caggacatat taaagtttac attgaaccag ataattctat tacagttgtt
     2281 gatgatggtc gcggtattcc ggtagatatc caagaaaaaa ctggaagacc agctgtagag
     2341 acagttttta cagttctcca tgccggtggt aaatttggtg gtggcggata caaggtttct
     2401 ggtggcttac atggtgtagg gtcatctgtt gttaatgcat tgtcaacaca attagatgtc
     2461 aaggtttata aaaatggcaa gattcattat caagaatacc aacgcggtgt tgttgttaat
     2521 gatcttgaaa ttatcggtga tactgactta tcaggaacaa cagttcactt tacgcctgac
     2581 ccagaaattt tcactgaaac cactgtattt gattttgata aattagccaa acgtattcag
     2641 gaattagctt ttttaaatcg tggactaaga atctccattt cagacaagcg agaaggtcaa
     2701 gaagttgaaa aagaatatca ctatgaaggt ggtataggtt catacgttga gtttatcaat
     2761 gagaacaaag aagtgatttt tgaaaatcct atttatactg acggtgaatt agatggtatt
     2821 tcagttgaag tagcaatgca gtatactact ggatatcaag agactgttat gagttttgca
     2881 aataacatcc acactcatga aggaggaacg cacgaacaag gatttcgtac tgccttgaca
     2941 cgtgtgatta atgattacgc taaaaagaat aagattctca aagaaaatga agataattta
     3001 actggagaag atgttcgtga aggtttaaca gcagttatat ctgttaaaca tccaaatcca
     3061 caatttgaag gacaaactaa aacaaaactt ggaaattctg aagttgttaa aattacaaat
     3121 cgtctcttta gcgaagcatt caatcgattc ctattagaaa atccacaagt agctaagaag
     3181 attgttgaaa aaggaatttt ggcatctaaa gcccgtattg cagcaaaacg ggcgcgtgaa
     3241 gtaacacgta aaaagtcagg cttagagatt tcaaacttac ctgggaaact ggctgattgc
     3301 tcatctaata atgctgagat gaatgaactt tttatagtgg agggagattc tgcgggaggg
     3361 tctgcaaaat caggacgtaa tcgtgagttt caagcaattt taccgattag gggtaagatt
     3421 ctaaacgttg aaaaagctac aatggataaa attttagcaa acgaagaaat ccgaagtctc
     3481 ttcacagcta tgggaactgg atttggtgca gattttgatg tatcaaaagt cagatatcaa
     3541 aaattagtca ttatgactga tgccgatgtt gatggagctc acatcagaac tttactttta
     3601 actctgattt atcgttttat gcgacctgtt ttggaagcag gatatgttta tatcgctcaa
     3661 ccaccaatct atggtgtcaa agtaggaagt gagattaaag cttatattca gccaggggtc
     3721 aatcaagaag aggaattacg tcaagctcta gatacctata gttcaggacg ttcaaaacct
     3781 actgtacaac gttacaaagg tctaggtgag atggatgacc atcaactttg ggaaacaaca
     3841 atggatcctg aaaaccgctt gatggcacgt gtctcagttg atgatgcagc agaagcagat
     3901 aagattttcg atatgctaat gggagatcga gtagaaccac gacgtgaatt cattgaagct
     3961 aatgctgttt atagtaactt agatatctag tccaaagata gctttgaaaa acagttcaag
     4021 tatgctataa tcttaacgtt atagtagatt tttagaaaat tagaccatta taaggagagt
     4081 tgaatgtcga gcggaatcat attgctgctt gtggctatag tcctgcttgt gattattgcc
     4141 tacgtagtcg gcgtcgtaat aagaaaacgt aatgatacat taattgcaaa cttagaaact
     4201 agaaaacaag agttagttga ccttcctgtg caagaggaga ttgagcaagt caaattgctt
     4261 catttgattg gccaaagtca atcaactttt agagaatgga accaaaaatg gactgattta
     4321 tcaactaatt catttaaaga tattgatttt catttagttg aagctgaaaa tcttaatgat
     4381 tcatttaatt ttgtgcgtgc taagcacgag attgataatg ttgatagtca gctaactatt
     4441 atagaagaag atattgtttc gattcgtgaa gctttagaag ttttaaaaga gcaagaagag
     4501 aaaaatagtg cccgtgtaac acatgctttg gacctatacg aaacacttca aaaatcaatt
     4561 tctgaaaaag aagataatta cgggacaacc atgccagaaa ttgaaaagca gttgaaaaat
     4621 atcgaggctg aattttcaca ttttgtgacc ttaaactcaa ctggtgaccc gattgaagct
     4681 tctgaagttt tgaataaagc agaggagcat acgattgctt tagggcaaat tacagaacaa
     4741 attccagcta ttgttgctaa attagaagac gattttccag atcagttaga tgatttggag
     4801 acaggatatc gtcgtctttt agaggaaaac taccatttcc ctgaaaagga tattgaacaa
     4861 cgttttcaag aagttcgtga ggctattcgt tcgaattcag atggtttagt tagtttggat
     4921 ctagatcgtg ctcgcgatga aaatgaacat attcaagaaa aaattgataa actttatgat
     4981 atttttgaac gtgagattgc tgcttataaa gtagctcata aggatagtaa aattattcct
     5041 caattccttg ctcatgcaaa atctaacaat gaacaactgg ggcatgaaat caaacgatta
     5101 tctgctaaat atatcttaaa tgaaaatgaa agcttaagtt tacgttcctt cacaaatgat
     5161 ttggaagaaa ttgaaacaaa ggttttacct tctgtagaaa attttggtca agaagcttca
     5221 ccttatactc atctacaaat tttatttgag cgtacgttga aaacattgac tactgttgaa
     5281 gaaaatcaaa tggaagtttt tgaagccgtt aaaactattg aaagcgttga gacacgagca
     5341 cgccaaaata tggataagta tgttaacaag ctccatatga ttaaacgttt catggaaaaa
     5401 cgcaatcttc caggaatccc gcaagacttc ttaagcactt tctttacaac aagttctcaa
     5461 attgaggcac ttattaatga actcagtcgc ggtcgtattg atattgaggc tgtttcacgt
     5521 ttaaatgatg tgactacaaa tgctatcgct aatttagagc aagctacata tctagttgtt
     5581 caagatgcga ctctcacaga gcagttacta cagtattcaa accgttatcg tagttttgaa
     5641 caaaatgtgc aaaagagctt tgaacaagct ttgtacttat ttgaagttga acataactat
     5701 aaagcatctt tcgatgaaat ttcatacgca cttgagactg tggaaccagg tgtaactgac
     5761 cgttttgtga cgtcttatga aaagacacaa gaacgtattc gtttctaaaa aagaagagtt
     5821 agtactcttc tttttttaat tgctatctat gaaaggtgct aattttaagg tacaatagta
     5881 aaaaagtttt ggaggtttta aatgattaaa ggtttgttag tcatggacgt tgattcaacc
     5941 ttaataatgg aagaagctat tgatttatta gctattgaag caggggtggg gaaacaggtt
     6001 gctgcactga ctgacgctgc aatgcgtggc gagttagatt ttgaagaagc tttgaagaaa
     6061 agagtagcgc tacttaaagg tttgcctgtt actattttga cagatatatt atcctcaata
     6121 cattttactc caggggctta tgaattgatt aaagagtgtc acaagcgtca gatgaaagtt
     6181 ggtcttgtat caggaggttt tcacgagacg attgatatct tagctaagca attacaagtt
     6241 gattatgtca aagcgaatcg tcttggtgtc aaaggtggtt ttctcactgg cgaagttgag
     6301 ggagagattg tcactaaaga agtgaaaaaa ataaagttaa aagagtgggc ttctgaaaat
     6361 cacttagacc tttcacaaac catcgcaatg ggagatggtg ccaatgattt accaatgatc
     6421 aaatccgctg gggtaggaat tgctttttgt gcaaaaccaa ttgtcagaga agaagctgcg
     6481 tatcagatta atgaaaaaaa tttgcttaag gtatttgata ttattgatgc aaatgaaaac
     6541 taggccttta ttaagcctag tttttagatt aaggatttta agagttttat ttgagagggt
     6601 attaatttgt ttttagataa aaataaatca atatattctg gttttaacca gatatagtca
     6661 acggtctctc cttcttgcaa tgtaattata tcttttgcac cattgtattt tgcgataaaa
     6721 tggtctacaa agtaagaacg ctcagagaac cactctctat taagaaaggt tagacattga
     6781 ggaacgatac cagtttcttc tttgagttcc cttagaatag cttgcttagc attttctcct
     6841 ttaagggcag atccgccagc ggtggcttcg aaataagcag gaaaagcttt ctttttaggg
     6901 tgccgtttca tcatcaatac gtcaccatcc tcatgaaaaa ggataacatc aacaactaag
     6961 tggaataagc cttgtgggat attttgatcc ctattaagtg taagacctgt aatgaaccca
     7021 tattggtcat aagcatccca aatttccatt agacctcctt gttatttttg actaaagaaa
     7081 tgttgccata gacttttttt aggagactcc tctttttttg gtgaattgaa taaatcaggg
     7141 aattgtgtct caaaatcgtt ccattcaacg ttttcagcct tatcactatg tataataata
     7201 ccaaatggag aattggcatt tgcttcatta acaatcgtag cagctagtga tagacggtta
     7261 gctaacttca tgtagaacat ttgaacatca gtagggagat ttggtgataa ttttaaacta
     7321 agattaggat attcaagtat cagattatta agagcagatg ttatagcaag ttgaaacttt
     7381 tcttcttgtg cctcttttaa acttaagtgg agcacaactc tttctttata agtaccaagg
     7441 tagtaacgtt gttgatcagg atcaagacga ttttcaccgc ttgctttttg taggatttta
     7501 ttgtcaagtt cagtcataat ttctccttga atttgctaat tttattataa cctaaatggg
     7561 ttgcattagc acaggtaaac ttgaaaagaa ttagtgattt ttttatcaaa aaccacgtaa
     7621 taaagcggtt tcactgggtt tttccttgtt attaactaaa gattatgata ttatgaatat
     7681 gtaaaaaatt aaaactcata aggagagtat tgtaatgtca attattactg atgtttacgc
     7741 acgcgaagtc cttgactcac gcggtaaccc aacacttgaa gtagaagttt atacagaatc
     7801 aggtgctttc ggacgtggta tggttccttc aggagcttct actggtgaac acgaagcagt
     7861 tgaacttcgc gacggcgaca agtctcgtta cagtggtctt ggaacacaaa aagctgttga
     7921 caatgtaaat aacgttattg cagaagcaat cattggttat gatgtacgcg accaacaagc
     7981 tatcgaccgt gctatgatcg ctcttgacgg aactcctaat aaaggtaaat taggtgcaaa
     8041 tgctattctt ggtgtttcta ttgccgtagc acgtgctgca gctgattacc ttgaagtacc
     8101 actttacagc taccttggtg gattcaacac taaagttctt ccaactccaa tgatgaacat
     8161 catcaacggt ggttctcact cagacgctcc aatcgcattc caagaattca tgattatgcc
     8221 agttggtgca ccaactttca aagaagcact tcgttggggt gctgaagtat tccatgctct
     8281 taagaaaatc cttaaagaac gtggtcttga aacagctgtt ggtgatgaag gtggatttgc
     8341 acctaaattt gaaggtactg aagatggcgt agaaactatt cttaaagcta ttgaagctgc
     8401 tggttacgaa gctggcgaaa acggaatcat gattggtttt gactgtgctt catcagagtt
     8461 ctacgatgca gaacgtaaag tatacgacta cagtaaattc gaaggtgaag gtggagcagt
     8521 tcgtactgct gcagaacaaa tcgattacct tgaagagttg gttaacaaat acccaatcat
     8581 cactattgaa gatggtatgg atgaaaacga ctgggatggt tggaaagctc ttacagaacg
     8641 ccttggtgga cgtgtacaat tagttggtga cgatttcttc gttacaaata ctgattactt
     8701 agcacgtggt atcaaagaag aagctgctaa ctcaatcctt atcaaagtta accaaatcgg
     8761 tactttgaca gaaactttcg aagctattga aatggctaaa gaagctggtt acactgcagt
     8821 tgtatcacac cgttcaggtg aaactgaaga ttcaacaatc gctgatatcg cagttgcaac
     8881 taacgctggt caaattaaaa caggttcatt atcacgtact gaccgtattg ctaaatacaa
     8941 ccaattactt cgtatcgaag accaacttgg tgaagttgct caatacaaag gtatcaaatc
     9001 attctacaac ttaaaaaaat agtctcagat tattaaacag ttgaaaatat aataaaaacc
     9061 atcttgcata tgcaagatgg tttttatgta cacattataa taatagaatt gatttgataa
     9121 taaacagttc taccattgca atccagcagc agcgatttct gcttttgaag cgtattgtcc
     9181 atttggacga tgccaccttc ctctatagtc acgtttgtag ccttgactag tttgttcttg
     9241 tactagagtt tcttgttttt tctgagtttc agctgcgagc cttgcttgct cctctttttg
     9301 cttcctttga gcttcagcag caagccttgc ttgttcttct tccagttttt tctgagtttc
     9361 agcagcagct ttttcctctt caactttctt ttctgcaagt cgtttttctt cttcgtgctt
     9421 agcgataagt tttctttgtt ctttagctga taagacagta ttttgagcct ttccgctgag
     9481 gtagtcaatg gtagcatttg gtgaggtatt ctctaaggta acagtgctga tgccgaattg
     9541 atcttgagtg gatttgtttc cgataaaaat aggtaataat ttcccagtct tatcaatacc
     9601 aacatatttt aattcaattt ttcttggaat tagttcatta ttttgataaa ttggtgttac
     9661 tttataatct aagtagtagt ttttatgagt cgaaagccaa gtctttagtt gtttttcata
     9721 gaatagcatg ctctcagggt tagatgaatt cgtgctattg tagtttccag tatttagcca
     9781 attcgtcatt ggaactaaat tctttctttc attgtttagt ccactaaatt ggtggcaaat
     9841 aagacggcct cggctcatta gccaagcttt ttgagtacca tcattataat agaatttaaa
     9901 attgtgccat ccaacagggg ttgtttttaa acgttttact ctctttttct tgggttcatc
     9961 tttaactttt agctgaatat gtgcgaatgt ggcacgacct ttgtcatcca actctcctaa
    10021 aaccaattgt cgtttgtgtt taaaaggtaa agtaccattc tcttttgttt tatactcttg
    10081 agcagtaaca atattttctg tggtatgatt tgtagctagg tttattggtg tgtaaagcga
    10141 aataaccgtt agtaaggttg caattcctaa ttttataaac tgttttcttt tcatctgtta
    10201 acctctaaaa taacataaaa tagtagctgt ttatttttaa atgcattctg aaaattaaaa
    10261 tgtgattttt gtaacaatat tataacataa tgatgtatga catgtgaagt taatttataa
    10321 aagatgttac agctatatag aggagctata gtataaataa aaaattcaaa atatagcata
    10381 tattatgata caataatagc aaaatagatt ttgggagtta tcatgaaatt actgactaat
    10441 gcaaatactc tcaagggaac catccgtgtt cctggcgata aatcaataag tcacagggct
    10501 attatttttg ggagtatatc tcagggagtt acccgaattg ttgatgtctt aagaggcgag
    10561 gatgttttat caactataga ggcttttaaa caaatggggg ttctaattga agacgatggc
    10621 gagataatca ctatctatgg caaaggattt gcaggtttaa cacaaccgaa taatctctta
    10681 gacatgggaa actctggtac ttcaatgcgt ctgatagcag gtgtgttagc tggtcaagag
    10741 tttgaagtga cgatggttgg agacaatagt ttgtcaaaga gacctatgga tcgtatagca
    10801 ttgcctttaa gcaaaatggg agctagaatt tcgggagtaa ctaaccgtga tttaccacca
    10861 ttaaaacttc aaggtacaaa aaaattaaaa cctatttttt accatttacc cgtagcttct
    10921 gcacaagtaa aatcagcttt aatttttgcg gctttacaaa ctaagggaga atctctcatt
    10981 gtagaaaaag agcaaacaag gaatcatact gaggacatga ttcggcaatt tggtggacat
    11041 ttggatatta aagataaaga aattcgactc aatggcggtc agagcttagt aggacaagat
    11101 ataagagttc ctggtgatat ttcgagtgct gccttttgga tagtagcggg tcttattatt
    11161 cctaacagtc atattatttt agaaaacgta ggtataaacg agacacgaac tggaatcctt
    11221 gatgtcgtca gtaaaatggg tggaaaaatt aagttatctt cggttgataa tcaagtaaaa
    11281 tcagcaaccc ttacagttga ttactctcat ctacaagcta ctcatatttc tggggctatg
    11341 attccacgtc tgattgatga attacctatt attgctcttt tagcaacaca agctcaaggc
    11401 accacagtca tagcagatgc tcaggaatta aaggtgaaag agacagatcg gattcaggtc
    11461 gttgtcgaat cgctaaaaca gatgggtgct gatattactg ctacagcaga tggtatgatt
    11521 atacgtggta atacccctct tcacgctgct tcacttgatt gtcatggaga tcatagaata
    11581 ggaatgatga tagctattgc tgctctcttg gtcaaagaag gcgaagtaga tttatctggt
    11641 gaagaagcta ttaatacaag ctatcctaac tttttagaac atttggaggg attagttaat
    11701 gcctaaggta ttattgggat tcatgggagt tggcaagact agtgttgcca actgcttaga
    11761 aaatgaagta attgatatgg atagtctgat tgaaaaacat attggtatgt caatttcccg
    11821 attttttaca gaagaaggag aagcctcctt tcgtgcttta gaatctcaat ttttaaacga
    11881 acttttaaag aaaaaaaatg aaggacttgt gatagctagt ggaggaggta ttgtactcct
    11941 tgaagaaaat cgtcgtcttt taactttaaa tcgtcataat aatattcttt taacaggaag
    12001 ttttgaaatc ttatatcatc gtataaaaaa agatgagaag aatagaagac cattattttt
    12061 aaaccattca aaagaagaat tttatgatat ttatcaaaaa cgcatgttac tctatagtgg
    12121 tttgtctgat atgattattg ataccgatta tcttacacca caaaaaatag ctactgtaat
    12181 tggagagtca ctggttaatt tatctaacta agttatcaca gtttggtagt atgaactatt
    12241 attattgaaa gtaaatcacc tgaaaatatg tcaagaaata attacggtca gctaaatcat
    12301 catgaagagc tacgatataa ctatctttta aaaaacatcc attatcttaa tgaacgtgaa
    12361 aagatggaat ttcagtacct tcattataaa aaaacagcag tacgaccaca gagaaggaca
    12421 gaaagtccac caactaattc ttattatgag gaaccttatt ctgattctta ttatcaagat
    12481 gacgattttt actcagagcc tcagctaacc tcacaaggtt taccaattta tcaagaagag
    12541 agagctccga agaagaaaaa acaaagggcc agaaaagaaa aacagagggt gaaggttatg
    12601 gcgcctttcc ctccaaaggc tataaccccc cctagaaaga aaaaaaaatt taagggtttc
    12661 ctaaagttca taggtattat attactaatt gttttatcag gtatggtttt catgtttgtt
    12721 aaaggaatgc gtgatgtgaa taatgggaag tcacattata gccctgcaat tattgaagat
    12781 tttaagggaa aagatgctgt tgatgggact aatatcttaa ttttaggtag tgataaacgt
    12841 atcagcgaga gatctactga tgcgagaaca gataccataa tggttgctaa tgttggtaac
    12901 aaggataata aggttaagat ggtgagtttt atgcgagatc tcttagtcaa tattcctaat
    12961 tatagtacgg aaggttacta tgatatgaaa ttaaatgcgt cttttaattt gggagaacaa
    13021 gataatcata aaggagcaga gtatgttcga caaacgttga aaaatcattt tgatattgat
    13081 ataaaatatt atgttatggt tgactttgag acatttgcag atgctattga tacactgttt
    13141 cctaatggtg tcaagattaa tgctaaattt ggacttgtcg gtggacaatc tgctgattca
    13201 gttaaagttc ctgatgattt aaggatgaaa aatggcgttg tacctagcca aaaaattaag
    13261 gttgggattc agtatatgga tggaaggacc cttctaaatt atgctcgttt cagaaaagac
    13321 gatgatgggg actttggtcg aacacaacgt caacaacaag ttatgagagc tattgtctct
    13381 caaataaaag acccacgtcg tctctttaca ggttctgctg ctatcggtaa agcctatgct
    13441 ttaacttcaa gtaatttatc ttatagcttt gtcctaacag atggcattcc aattttatca
    13501 gatgctaaaa atggtattaa gcaaatgaca attccaagag aaggagattg ggtagatgat
    13561 tatgaccagt atggtggtca agggttaaca attgattttg ctaagtacaa aaagatccta
    13621 aagaaaatgg gactgcgtta acatttttaa ttatgatata atagagtgat ttattgtcac
    13681 tctattttta tctttagtgt ataaactaaa gacttcaaat gatagatata aaaggaaaat
    13741 tatgttacat aaaaatgata ttattgaaac tgaaattagc gacattagcc atgagggaat
    13801 gggtattgcc aaagtagatg gctttgtttt ctttgttgaa aatgctcttc caggtgagat
    13861 tattcagatg cgcgtcttga aattacgtaa gcgtatagga tatggaaaag ttgaggaata
    13921 tttaacgact tctcctcacc gaaatgaagg attagactat acctatttac gtacagggat
    13981 tgctgatttg ggacatctta cttatgaaca acagctgtta tttaaacaaa aacaagtagc
    14041 tgataacctt tataaaattg ctcatatatc tgatgtttta gtagaaccta ctcttggtat
    14101 gacaatccct ttggcatatc gaaataaagc tcaggtacca gttcgtcgtg ttgatggaca
    14161 gctagagaca ggttttttca gaaaaaattc acatactctg gtatccattg aagattattt
    14221 aattcaggaa aaagagattg atgctctgat taattttaca cgagatttat taagaaaatt
    14281 tgatgttaaa ccatatgacg aagagcagca atctggttta attagaaatc tcgttgttcg
    14341 tcgaggacat tatacagggc aattaatgtt ggttttggtg acaacacgtc ctaaaatatt
    14401 ccgcattgac caaatgattg aaaagttggt gtctgccttt ccttctgttg tttcaattat
    14461 gcaaaatatt aatgatcgta atagcaacgt tatttttggg aaagaatttc gtactctata
    14521 tggttctgat actattgagg accaaatgtt aggaaatact tacgctatat cagcccaatc
    14581 tttttaccaa gttaatactg aaatggctga aaaactgtac caaaaagcaa tcgatttttc
    14641 agatttaaac tcagaggata tcgttattga tgcctattca ggtataggaa caattggctt
    14701 atctgttgct aaacaagtaa aacatgttta tggtgttgaa gttgttgaaa aagcagtaag
    14761 tgatgccaaa gaaaatgcta ctcgaaatgg gataactaac agtacctatg ttgctgattc
    14821 ggctgaaaat gcaatggcaa agtggcttaa agaaggtata aaaccaactg ttataatggt
    14881 tgatccacca cgaaaaggat taacggagag ttttgtatat tctgctgccc aaacaaaagc
    14941 agacaaaatc acttatattt cttgtaattc agcgacaatg gcgcgtgata tcaaactatt
    15001 tgaagaatta gggtatcatt tagtaaaaat tcaaccagta gacttattcc caatgacaca
    15061 tcatgtggag tgcgtagcgt tgctacaacg aagcaaaggg taaaaatcct ttattttaag
    15121 cactttttca agcattttgt ctttattgaa aagagtgatt ttaacataaa aaaggtatta
    15181 aaaaacatat tgacgtgacc gtttgttttg aagtggcttg cgtagacaaa aaaatagata
    15241 cgtcagataa atttctggca ttacgagaac atttttagag tgttctcttt ttttagttta
    15301 cggaggaaaa atatatatgg aaaaacagga ttcacgagtt ctcatccatt gggagggaaa
    15361 ttctggggac aagcttattg aacaccaaac cagcgcaacg gggtggtact accaagtcga
    15421 tcgtagcttt agtcaaccaa aaggggaacc gcccagaatg atccaaaggc actagaaagt
    15481 gtccgtaatg attcgatttc gggcggtgat gatgtcatgg gttatgctta tagcaaatgt
    15541 acttggggag ttgcggcacg aattaatcag tgggactgaa actcaaaggt tgaaatggtg
    15601 agaagattac cattaccagt tcaatgggaa atggtcagga ttgggttgga acagccgaaa
    15661 gactagatgg tgaaactgat acagttccaa aagaaggtac tattctctct ttttaggaaa
    15721 gtagttatgg ttcgtatata ggctacggaa ctatatcttt cgtcacatta catctacaga
    15781 tagtaccatg aattttgctt atatgaccaa gtaaagtgag gatatactaa caaatgaaat
    15841 atttattatc gtatttgtcc attttatcga aaagtttgca tattatcatt atgtttgata
    15901 agatgcaaat ataatgatag taggagctaa atatggatat ttaaaaaatc aagagtatcc
    15961 taagtgcttt ccattttgaa attcaaatat agctaatagt tctagaactt ctaattgttt
    16021 ttcgtcgacg atatgaattt tcaatcttaa ctgttaggat tccacctccc tttggttaaa
    16081 gaaaaaaggt caggtcgttt agataacttt gtcaaacaag ctcaagctat ctaaaaatag
    16141 tttgaaatgg gcattactct agtttttaat aagctatctg atgagcagaa ggagaagtta
    16201 atgcatgttg ggaagtctta ttttgactat caagaaaatg ctcttatccc acaattaggt
    16261 tttctatatt ctaaattaac taaaaaaatt gaacttgata atcggttgtc tccgactgaa
    16321 caaaagttat tgattacctt attattacat actaaaggtt taatcattga tat
//
LOCUS       DATRTN010000037        15894 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.037, whole genome shotgun sequence.
ACCESSION   DATRTN010000037 DATRTN010000000
VERSION     DATRTN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15894
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.037"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..915)
                     /locus_tag="VBE22_001160"
     CDS             complement(<1..915)
                     /locus_tag="VBE22_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7202627.1"
                     /translation="MNWSRIWELVKINILYSNPQTLSALRKKQEKHPKKEFSAYKSMF
                     RNQLFQILLFSIIYVFLFVSLDFKEYPGYFTFYIGIFTLVSIIYSFIAMYSVFYESDD
                     VKQYAYLPIKSEELYVAKIFATFGMSVTFLMPILTLMIVAYWRIIGGPLAVLLAIINF
                     AILFLSVTVISLYINSLIGRAIIRSANRKLISTILISLATFGAIVPLLFVNMTSQKMV
                     QGKLQDIAPIPYVRGYYDIVTAPFSMESLLNYYLPLLIILFLIGAIYKWVMPRYYQEL
                     LYGQVKQRKVHRQIDFSKRESINKTLVKH"
     gene            complement(919..1653)
                     /locus_tag="VBE22_001161"
     CDS             complement(919..1653)
                     /locus_tag="VBE22_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202628.1"
                     /translation="MIKFEHVSKVYGEKEALSDLTLSIKDGEIFGLIGHNGAGKTTTI
                     SILTSIIDATYGQVYIDDLLLTEHRDQIKKKIGYVPDSPDIFLNLTAEEYWYFLAKIY
                     DVAPEDIEARITKLVDIFELEEQRYNPIESFSHGMRQKVIVIGALLPNPDIWILDEPL
                     TGLDPQASFDLKEMMKEHAKNGKTVIFSTHVLAVAEQLCDRIGILKQGKLIFVGSLGE
                     LKMQYPDKDLETIYLELAGRQANREG"
     gene            complement(1673..1978)
                     /locus_tag="VBE22_001162"
     CDS             complement(1673..1978)
                     /locus_tag="VBE22_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO7202629.1"
                     /translation="MKKECRDFYRQIQHTYNDIAVREDAVLSSILLSASNGLIKTSDV
                     PRVAYELTQQLENNEIEKSFESLATVKELKKSAKKLSERYEQNKVTTGLLSKVFGNH"
     gene            complement(2191..4176)
                     /gene="tkt"
                     /locus_tag="VBE22_001163"
     CDS             complement(2191..4176)
                     /gene="tkt"
                     /locus_tag="VBE22_001163"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO7202630.1"
                     /translation="MTFDTIDQLAVNTVRTLSIDAIQAANSGHPGLPMGAAPMAYVLW
                     NKFLNVNPKTSRNWTNRDRFVLSAGHGSALLYSLLHLAGYDLSIDDLKQFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDLVDHYTYTLHGDG
                     CLMEGVSQEAASLAGHLKLGKLVLLYDSNDISLDGPTSQSFTEDVKGRFESYGWQHIL
                     VKDGNDLEAIAAAIEAAKAETDKPTIIEVKTIIGFGAEKQGTSSVHGAPLGAEGITFA
                     KKAYGWEYPDFTVPAEVVARFASDLQARGAKAEEAWNDLFAKYEVEYPELAAEYKEAF
                     AGQAETVELKAHDLGSSVASRVSSQQAIQQLSTQLPNLWGGSADLSASNNTMVAAETD
                     FQASNYAGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWQRAVSET
                     DRPTMLVLTRQNLPVLEGTSELAQEGVNKGAYILSEAKGELDGIIIATGSEVKLALDT
                     QDKLESEGIHVRVVSMPAQNIFDEQEASYQEQVLPSAVTKRLAIEAGSSFGWGKYVGL
                     NGLTLTIDTWGASAPGNRIFEEYGFTVENAVSLYKEL"
     gene            complement(4301..5731)
                     /locus_tag="VBE22_001164"
     CDS             complement(4301..5731)
                     /locus_tag="VBE22_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7202631.1"
                     /translation="MRIEALMEKERRVQYRLLSFLRGSPQAIALKLALLETGLSRATL
                     LKYINNLNSYFEQEKVNCRIVYYKDKLFLEEDYNLSNQEVLKALMKDSIKYTILISLF
                     NQRQFTIVGLSQELMVSEATLNRHLAHLNELLAEFDIAISQGKQIGDELQWRYFYYEL
                     FKQLWSYDKCQNMIKKLDLDSLILLIERLAQHTLTREAQQNLGLWFSICHHRLLAMEK
                     ISDNLKPIVEHYQCNAFYKRLDAALVLYMSRFALEYREGEVLATFAFLHSQNILPINT
                     MEYIMGFGGPIIDCVTETIIYFKKESILADETSDQVIYQLGQLYSHYYFFKGHILVEQ
                     PDLEQTYRLIDHNMRDKLHHISKKIIANVNRIRPLTEGGCSLLTLHLLELLIFSKNSQ
                     KMPFRIGLDMTGNAVEQSLLEYRIRQHFSGNNSIQVEPYDEGKGFDMVIYQSRSRPYK
                     AKLTYCLNKGASERELQEIDSLIYDN"
     gene            complement(5722..7059)
                     /locus_tag="VBE22_001165"
     CDS             complement(5722..7059)
                     /locus_tag="VBE22_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7202632.1"
                     /translation="MTEKYVNKVIVILGASFAGMTCAQKLRQLNPNWDIVLIDKEIHP
                     DYVPNGLNWYYRHEISGLNQAMWQTEEEQRLQNIRCLFGLKVEKINKEDRELMLSDGS
                     SVYYDQLICAMGSQAESTYIDGADAQGVLTTKTYATSQNAKQVLDKSHKVAVVGAGII
                     GLDIAYSLHESGKAVTLLEAQERPDFRHTDPDMSLPLLDAMAESKLHFFQNQKVEKIT
                     VTREEKLCLRTLTGDTFTVDAVILAVNFRPDSRLLTGLVDLSVDNSVVVNDYFQTSDP
                     NIYAIGDLIWSYFKGLNSAYYMPLINQAIRSAQMLAYHLSGHAVPKLKITRATGSKHF
                     GYYRANIGLTELEAGLYEDTVSVTYFPKEQYDLRIKLIANQKTGHLLGAQLISKENCL
                     ATANQLVQAISCDMTDFDLAFQDFIYTARESEMAYMLHQAAINLYEKRIGLCE"
     gene            complement(7147..7845)
                     /locus_tag="VBE22_001166"
     CDS             complement(7147..7845)
                     /locus_tag="VBE22_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO7202633.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKNHNSGWIVITFGWG
                     LAVAIAVLVSGNISPAHLNPAVSLAFAIKGDLAWGTAILYMIAQIIGAMLGSLLVYLQ
                     FRPHYEAAENKADILGTFATGPALKDNFSNFLSEVLGTLVLVLTIFAIGKYNMPPGVG
                     TMSVGMLVVGIGLSLGGTTGYAINPARDFGPRLLHALLPMKNKGDSDWTYSWIPIVGP
                     MVGAILAALIFAMM"
     gene            complement(7857..9686)
                     /gene="glpO"
                     /locus_tag="VBE22_001167"
     CDS             complement(7857..9686)
                     /gene="glpO"
                     /locus_tag="VBE22_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEO7202634.1"
                     /translation="MEFSRETRRLALQRMQDRTLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVTNTPAANKVLSAEDVLKREPDLQKEGL
                     LGGGIYLDFRNNDARLVIENIKRANRDGAYIASHVKAEDFLFDDNNQIIGVRARDLLT
                     DQVIDIKARLVINTTGPWSDTVRNFSNEGKQIHQLRPTKGVHLVVDRQKLNVSQPVYV
                     DTGLNDGRMIFVLPREDKTYFGTTDTDYHGDLEHPTVTKEDVDYLLNIVNKRFPEAEL
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKLSDESFEELIDSVKDYIAHKNHREDVE
                     KAISHVESSTSEKELDPSAVSRGSSFERDDNGLLTLAGGKITDYRKMAEGAMETIINI
                     LDKEYNRKFKLINSKTYPVSGGEINPSNVDSEIEAYAQLGTLSGLSIEDARYIANLYG
                     SNAPKLFALTRQITEAEGLSLVETLSLHYAMDYEMALSPTDFFLRRTNHMLFMRDNLD
                     SLIQPVIDEMAKHYQWSDQDKTFYEEELHETLKDNDLAALKDN"
     gene            complement(9699..11207)
                     /gene="glpK"
                     /locus_tag="VBE22_001168"
     CDS             complement(9699..11207)
                     /gene="glpK"
                     /locus_tag="VBE22_001168"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEO7202635.1"
                     /translation="MSSEEKYIMAIDQGTTSSRAIIFNKKGEKIASSQKEFPQIFPQA
                     GWVEHNANQIWNSVQSVIAGAFIESSIKPGQIEAIGITNQRETTVVWDKKTGLPIYNA
                     IVWQSRQTAPIADQLKQEGHTNMIHEKTGLVIDAYFSATKVRWILDHVPGAQERAEKG
                     ELLFGTIDTWLVWKLTDGLVHVTDYSNAARTMLYNIKELKWDDEILELLNIPKAMLPE
                     VKSNSEVYGKTTPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMN
                     TGEEMQLSQNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLRMIETSSESEGL
                     AQSSTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRD
                     VIDTMQVDSGIDIQQLRVDGGAAMNNLLMQFQADILGIDIARAKNLETTALGAAFLAG
                     LSVGYWESMDELKELNATGQLFQATMNESRKEKLYKGWRKAVKATQVFAQED"
     gene            complement(11321..11584)
                     /locus_tag="VBE22_001169"
     CDS             complement(11321..11584)
                     /locus_tag="VBE22_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEO7202636.1"
                     /translation="MIELLLALLLWNILVFATYAIDKRRAIRGAWRISEKTLLIESLL
                     LGGFGAFLGGEVFRHKTTKWYFRLVWYIGMIIDLAIIWLIWCY"
     gene            complement(11673..11930)
                     /locus_tag="VBE22_001170"
     CDS             complement(11673..11930)
                     /locus_tag="VBE22_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEO7202637.1"
                     /translation="MDPKKIARINELSKKKKTVGLTGEEKVEQAKLREEYIEGFRRSV
                     RHHVEGIKLVDDEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(11942..13981)
                     /locus_tag="VBE22_001171"
     CDS             complement(11942..13981)
                     /locus_tag="VBE22_001171"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEO7202638.1"
                     /translation="MTKDLLLELGLEELPAYVVTPSEKQLGQKMVKFLEDHRLSFETV
                     QTFSTPRRLAVRVKGLADQQTDLTEDFKGPSKKIALDAEGNFSKAAQGFVRGKGLSVD
                     DIEFREVKGEEYVYVTKHETGKSAIDVLASVTEVLTELTFPVNMHWANNSFEYIRPVH
                     TLVVLLDDQALELDFLDIHSGRISRGHRFLGSDTEILSASSYEDDLRQQFVIADAKER
                     QQMIVDQIHAIEEKENISVEIDEDLLNEVLNLVEYPTAFLGSFDEKYLDVPEEVLVTS
                     MKNHQRYFVVRDRDGKLLPNFISVRNGNAEHIENVIKGNEKVLVARLEDGEFFWQEDQ
                     KLNIADLVEKLKQVTFHEKIGSLYEHMDRVKVISQYLAEKADLSDEEKLAVLRAASIY
                     KFDLLTGMVDEFDELQGIMGEKYALLAGEQPAVAAAIREHYMPTSADGELPETRVGAI
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWDIPLDELVTNLYG
                     LSFASLDYANQKEVMAFISARIEKMIGSKVPKDIREAVLESDTYIVSLILEASQALVQ
                     KSKDAQYKVSVESLSRAFNLAEKVTHSVSVDYSLFENNQEKALYQAILSLELTEDMHD
                     NLDKLFALSPIINDFFDNTMVMTDDEKMKQNRLALLNSLVAKARTVAAFNLLNTK"
     gene            complement(13985..14626)
                     /locus_tag="VBE22_001172"
     CDS             complement(13985..14626)
                     /locus_tag="VBE22_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7202639.1"
                     /translation="MNTLIVNSHPDFSNPYSFTTILQEKFIELYNEHFPNHQLSILNL
                     YDCVLPEITKEVLLSIWSKQRKDLELTADEIVQAKISKDLLEQFKSHHRIVFVSPMHN
                     YNVTARAKTYIDNIFIAGETFKYTENGSVGLMTDDYRLLMLESAGSIYSKGQYSPYEF
                     PVHYLKAIFKDFMAFDDFCVVRAEGTDILDRQVVLDKMNQDLREAFEAFYSKE"
     gene            complement(14626..15540)
                     /gene="glyQ"
                     /locus_tag="VBE22_001173"
     CDS             complement(14626..15540)
                     /gene="glyQ"
                     /locus_tag="VBE22_001173"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEO7202640.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNVQELYLKSLEL
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLQTGPVTSE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFTQPEYEHSKYSFEISDQVMLLENFE
                     KFEREAKRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEETRIKLLAEED"
BASE COUNT         5059 a         3321 c         2512 g         5002 t
ORIGIN      
        1 gtgtttaaca agagttttat taattgactc acgtttacta aaatcgattt gacgatggac
       61 ttttctctgc ttgacctgtc catacaacaa ctcctgatag tagcgtggca taacccattt
      121 ataaatagca cctatcaaga ataaaatgat taagagcgga agataatagt tgaggagact
      181 ctccatacta aacggcgctg taacaatatc ataataacct ctgacataag gaattggagc
      241 gatatcttgt aatttccctt gtaccatctt ttgactagtc atattcacaa ataataaggg
      301 aactattgct ccaaatgtag ctagtgaaat taatatagta gaaataagtt tccgattagc
      361 acttcgaata atggcacggc ctattaaact attaatatac aatgaaatta ccgttaccga
      421 taaaaataaa atagcaaaat ttatgattgc taggaggacc gcaagtgggc cacctattat
      481 acgccaataa gccacaatca ttaaggttag gattggcatt agaaaagtta cagacatccc
      541 aaatgttgca aaaattttag caacatagag ctcttcagat ttaataggca aataagcata
      601 ttgtttaaca tcgtcactct catagaaaac actgtacatc gcaataaaag agtagataat
      661 ggatactagt gtaaagatac caatgtagaa cgtgaaatag cccggatatt ctttaaaatc
      721 aagtgataca aagagaaata cataaattat tgaaaagagc aaaatctgaa ataactgatt
      781 tctaaacatg gatttataag ctgaaaattc ttttttagga tgcttttctt gcttttttct
      841 tagtgccgat agagtctgag ggtttgaata aaggatatta atttttacga gttcccagat
      901 acgtgaccaa ttcatacttt atccctccct attagcttga cgtcctgcta attccaaata
      961 gatagtttcc aaatctttat ctggatattg catttttaac tcacctaagc taccgacaaa
     1021 gattaatttc ccttgtttca aaatacctat tctgtcgcat aactgttctg caactgctaa
     1081 aacatgtgtt gaaaaaataa ctgtttttcc atttttagca tgttctttca tcatttcttt
     1141 caaatcaaaa gaagcttgag ggtctaaacc agttaacggc tcatctagaa tccaaatgtc
     1201 aggatttgga agaagggcac ctataacaat gactttttga cgcataccat gtgaaaaact
     1261 ttcaatagga ttatagcgtt gctcctctag ttcaaatatg tctaccaatt ttgtgatacg
     1321 tgcttcaata tcttctgggg caacgtcata aattttagct aaaaagtacc agtattcctc
     1381 tgcggttaaa tttaagaaaa tatctggcga gtccggtaca taacctattt ttttcttaat
     1441 ttggtcacga tgttctgtca aaagtaggtc atcaatatag acttggccat aagttgcatc
     1501 aataatagat gttagaatac taattgtcgt tgtttttcct gctccattgt gacctattaa
     1561 tccaaaaatc tcaccatctt taatagagag ggttaaatca ctgagagctt ctttttcccc
     1621 gtatactttc gaaacatgtt caaacttaat cataaaaatc tccttttatc tcctaatgat
     1681 ttccaaatac tttagaaagc aatcccgttg ttaccttatt ttgctcatat ctctcagaca
     1741 attttttagc agattttttc aattccttaa ctgtagctaa tgactcgaaa gatttttcta
     1801 tctcattatt ttccaattgc tgagttaact cataagcaac acgaggtaca tcgctagttt
     1861 ttatcaaacc attcgatgct gataatagta ttgatgatag cactgcatct tcacggacag
     1921 ctatatcatt gtaagtatgc tgtatctgcc gataaaaatc acgacactcc ttcttcataa
     1981 acaaaccttt ctaaaaagat aattaataat agttgtatta ttttatatat acaatagttt
     2041 tatcaattat actgcgctat ggaatgtttg tcaaacgttt tcaaaaaaga gtgctttcca
     2101 tcacctatca gtctatcaaa actattctca aatccatcct atatcttaca aaaaagaagt
     2161 tgaggtattt ttcccaactt cttcatgctc ttaaagttct ttatatagcg atacagcatt
     2221 ctcaactgta aagccatact cttcgaaaat tctgttacct ggtgctgatg ctccccaagt
     2281 atcaatagta agtgttagcc catttaaacc aacatattta ccccaaccga agcttgatcc
     2341 tgcctcaata gcaagacgtt ttgtaacagc tgatggaagc acttgctctt gatatgaagc
     2401 ctcttgttca tcaaaaatgt tttgtgctgg catagatacg acgcgaacat ggatgccctc
     2461 tgactcaagt ttgtcttgag tatctaaagc taatttaact tctgaacctg ttgcaatgat
     2521 gataccatca agttcacctt tagcttctga taaaatataa gcacctttat taaccccttc
     2581 ttgggctaat tctgatgttc cttcaagaac tggtaagttt tgacgtgtta aaacaagcat
     2641 cgttggtcga tctgtctctg atacagctcg ttgccatgca gcgtttgttt cattaccgtc
     2701 tgcgggacgg ataacgttca aattaggcat tgagcgcact gatgctaatt gttctattgg
     2761 ctcatgagtt ggcccatctt ctccaacagc aatagaatca tgtgtcataa catagacagt
     2821 tggcaagttt tgaagtgcag ccatacgtac tgctggtaac aagtagtttg agaagacaaa
     2881 gaatgtaccc ccataaacac gagtcccacc gtgaagcgca attccattca ttgcagcagc
     2941 cattgcaaat tcacgtacac caaaccaaat gttacgccca gcgtaattac ttgcttggaa
     3001 atctgtttct gctgctacca ttgtgttatt agaagctgag aggtctgctg accctcccca
     3061 gaggttcggt aattgagtcg ataattgttg aatagcttgt tggcttgaaa cacgactagc
     3121 cactgatgaa cctaggtcat gagctttcaa ttcaactgtc tcagcttggc cagcaaaagc
     3181 ttctttgtat tcagctgcta attctggata ctcaacttca tatttagcaa aaagatcatt
     3241 ccatgcctct tctgccttcg caccacgcgc ttgaagatct gaggcaaatc gagctacaac
     3301 ttcagcagga acagtaaagt ctggatattc ccatccataa gccttcttgg caaaagtaat
     3361 accttctgca ccaagtggcg ctccatgaac tgaagatgtt ccctgtttct ctgcgccaaa
     3421 tccaataata gttttaactt caataatggt tggtttatca gtttcagctt tagcagcttc
     3481 aattgctgca gcaatcgctt ctaaatcatt accatcttta actaagatat gttgccaacc
     3541 atatgattca aaacgccctt taacatcttc tgtaaatgat tgagacgttg gaccatcaag
     3601 tgagatatca tttgaatcat aaagaaggac caatttacca agttttaagt gtccagcaag
     3661 actggcagct tcttggctaa caccctccat caaacaacca tcaccatgaa gtgtataagt
     3721 gtaatgatca actaaatcaa agcctggttt attaaattta gcagccaaat gagcttcagc
     3781 cattgccata ccaacagcat tagcaatacc ttgccctaaa ggtccagttg ttgcttctac
     3841 accatcagta tggttaactt ctggatgtcc aggtgttttt gatccccatt ggcggaattg
     3901 ttttaaatca tcaattgata aatcatagcc agctaaatga agtaggctat aaagaagagc
     3961 tgaaccatgc ccagctgata gcacaaaacg gtcacggttt gtccaattgc gacttgtttt
     4021 tgggtttaca tttaagaatt tattccaaag cacataagcc ataggcgcag ctcccatagg
     4081 aagacctggg tgcccagaat ttgctgcttg gatagcatca atagaaagcg tgcggactgt
     4141 attaaccgct aattgatcaa tggtgtcaaa tgtcatgata aaacctttct gtttttaaca
     4201 actaaattta aaattgttcg tctttgatat tataacataa aatacgtaaa cgttttaaca
     4261 atttctggag ggattaaaac cattcaatta agtaatggga ttaattgtca taaataagtg
     4321 aatctatttc ctgtagttct cgctctgaag cacctttatt aaggcaatag gttagtttgg
     4381 ctttataagg tcttgagcga ctctgataaa taaccatatc aaatcccttt ccttcgtcat
     4441 aaggttcgac ttgaatagaa ttattaccag agaaatgttg tcgaatgcga tattccaaca
     4501 atgactgttc cacagcatta cctgtcatat ctaggccaat cctaaagggc attttttgac
     4561 tatttttcga gaaaataagc aattccaaca aatgtaaagt cagcaaagaa caaccacctt
     4621 ctgttaaagg tctaattcga ttaacatttg caataatctt ctttgaaata tgatgcaact
     4681 tatccctcat attatgatca ataagtcgat acgtttgttc taaatcaggt tgttcaacta
     4741 aaatatgacc tttaaagaaa taataatggc tatataactg accaagttgg taaataacct
     4801 gatctgatgt ctcgtcagct aaaatactct cctttttaaa gtagatgatt gtttcggtta
     4861 cacaatcaat aatcggacct ccaaacccca tgatgtattc cattgtattg attggtaaaa
     4921 tattttgtga atgtagaaaa gcaaaagttg ccagaacttc tccttcccta tactcaagag
     4981 caaaacgact catatacaaa actaaggctg cgtctaaccg tttataaaaa gcattacatt
     5041 gataatgttc cacaatgggc ttgagattat cagatatttt ttccatagca aggagacgat
     5101 gatgacaaat actaaaccaa agccccagat tttgctgagc ttctcgagtt aaagtatgtt
     5161 gagccaagcg ctctattaat agaattaatg agtctaagtc taatttcttt atcatatttt
     5221 ggcacttgtc ataagaccat agttgcttga ataattcata ataaaaataa cgccattgca
     5281 attcatcacc aatctgtttt ccttgtgaaa tagcaatatc aaattccgct aacaattcat
     5341 tgagatgagc aagatgacga tttaaagtag cctcactaac catcaattct tgagaaagac
     5401 caacgattgt gaactgacgc tgattaaata aagatattaa gatagtgtat ttaatactat
     5461 ctttcatcag agctttcaac acttcttgat tggataagtt ataatcctct tctaaaaata
     5521 acttatcctt ataataaacg attcgacaat taactttttc ttgttcaaag tagctattaa
     5581 gattattgat atatttaagc aaagttgctc gacttaaacc agtttctaat aaagctaact
     5641 tcagtgctat tgcctgagga gagcctctta aaaaagaaag tagccgatat tgaacacgcc
     5701 tttctttttc cattagagct tctattcgca tagcccaatc ctcttttcat aaaggttgat
     5761 agctgcttga tgtaacatat aagccatctc agattctcta gctgtataaa tgaaatcttg
     5821 aaaagccaaa tcaaaatctg tcatgtcaca cgatatagct tgaacaagct gattggcagt
     5881 agccaaacaa ttttcctttg agattaattg agcccccaat aaatggcccg ttttttgatt
     5941 agcaatcaat tttatcctta aatcatactg ttctttaggg aaataagtaa cactaacggt
     6001 atcctcgtaa agaccagctt ccagctcagt taatccaatg ttagctctat aatagccaaa
     6061 gtgtttagat cctgtagcgc gtgttatttt taattttgga acagcatgtc ccgataaatg
     6121 gtatgccaac atttgtgccg aacggattgc ttggttaatt aagggcatat aataagcact
     6181 attaagacct ttaaaatacg accaaatcaa atcgccaata gcatagatat tagggtcact
     6241 cgtttggaaa taatcattta caacaacact attatcaaca cttaaatcaa ccaacccagt
     6301 taaaagacga ctatccggac gaaaattaac agctaatatg acagcatcaa ctgtaaaggt
     6361 gtctcctgtt agcgttcgca aacaaagttt ttcttctcgg gttacagtaa ttttctcaac
     6421 cttttgattt tggaaaaaat gtagttttga ttcagccatc gcatcaagta aaggaaggga
     6481 catatcagga tctgtatgcc tgaagtctgg tctttcttga gcttctaaca aagtgaccgc
     6541 cttccctgat tcatgtaaac tataagcaat atctaaaccg atgatacctg ccccaacaac
     6601 tgctacctta tgacttttat ctaatacctg cttagcattt tgactagttg cataagtttt
     6661 tgttgttaaa acaccttgcg catcagcccc atcaatataa gttgattctg cttgggagcc
     6721 catggcacaa atcaattgat cataatagac agaagaacca tctgataaca ttaattccct
     6781 atcttcttta ttaattttct caacttttag accaaataga cagcgtatat tctgcaaccg
     6841 ttgctcttct tctgtctgcc acatggcctg atttaaacca ctaatttcgt gacgataata
     6901 ccaatttaag ccatttggaa cataatctgg atgaatttct ttatcaatca aaacgatatc
     6961 ccaattagga tttaattggc gtaatttctg cgcacaagtc ataccagcaa aagaagctcc
     7021 taaaatgaca attactttat ttacatattt ttctgtcata attacttatt ataacaaaag
     7081 aagttacttt tgaaaacggt aacaaaaaac tgagaccata aagtcggtct cagcatcaaa
     7141 aatctcttac atcatagcaa atattaaggc tgctaaaata gctcctacca taggtcctac
     7201 tatcggaatc catgaataag tccaatcaga atcacctttg ttcttcatcg gtaacaaggc
     7261 atgcaataaa cgaggaccaa aatcacgtgc aggattaata gcataacctg ttgttccacc
     7321 gagcgataga ccaatcccaa ccactaacat accaacactc atcgtaccaa ctcctggagg
     7381 catattgtat tttccgatgg caaaaatagt aagtacaaga acaagtgttc ccaatacttc
     7441 acttaaaaaa ttggaaaaat tatccttcaa agccggacca gttgcaaaag ttccaaggat
     7501 gtctgcttta ttttctgcag cctcataatg gggtctaaat tgcaaataaa caagaagtga
     7561 acctagcatt gcaccaataa tctgtgcaat catataaaga atagctgttc cccaagctaa
     7621 atcacctttg attgcaaaag ctaatgacac agctggattc aagtgggcag ggctaatatt
     7681 gccagatacc aacactgcaa tagctacagc aagcccccat ccaaatgtga taacaatcca
     7741 tccagaatta tgattcttcg ttttaggcaa aacaacacct gctacaacac cattaccaag
     7801 taagactaag agagctgttc ctaagaattc tccaaaaata tccattatta tctccttcaa
     7861 ttatccttca aagctgccaa gtcattgtct tttagagtct catgtaattc ttcttcatag
     7921 aaggttttgt cttgatcaga ccattgataa tgtttagcca tctcatcaat aacaggctga
     7981 atcaaagaat ctaaattatc tctcatgaaa agcatatgat tcgtacgtct taagaagaaa
     8041 tccgttgggc taagcgccat ttcataatcc atagcatagt gaagtgacaa cgtttctacc
     8101 aagctcagac cttctgcttc tgtaatttgg cgtgtcaatg caaataattt tggtgcattt
     8161 gacccataaa ggttagcaat ataacgtgca tcctcaatac ttaaaccact tagtgtgccc
     8221 aattgagcat aagcttcaat ttcagaatca acattagatg ggttaatctc tcctcctgaa
     8281 acaggatagg ttttagaatt gattagttta aacttgcgat tatattcttt atccaaaatg
     8341 ttaataatag tttccatggc accttctgcc atttttcgat aatcagtaat tttaccacca
     8401 gcaagtgtta atagaccatt atcatcacgc tcaaaacttg atcctcgaga aacagctgat
     8461 gggtccaatt ctttttcaga agtactagat tcaacatgag aaatagcttt ttcaacatcc
     8521 tcacgatgat ttttatgtgc aatataatct ttaacggaat caattaattc ttcaaaactt
     8581 tcatcgctta gttttccact attgcctcca ttatagtcag aggcactatt ccctgatagt
     8641 agtggacgca gacctgccca tgaactctca atatcatcaa tagtaagctc tgcttctggg
     8701 aaacgtttgt ttacaatatt aaggaggtag tcaacatctt ctttagttac cgtaggatgt
     8761 tctaagtctc catggtaatc ggtatctgta gttccaaaat aagtcttatc ttcacgtggc
     8821 aaaacaaaaa tcatgcgacc atcatttaga cctgtatcaa cataaacagg ttgagaaaca
     8881 tttaattttt ggcgatctac aactaagtga actcccttag ttggacgtaa ttgatgaatt
     8941 tgcttacctt cattggagaa gttgcgaacg gtgtcactcc aaggtccagt tgtattaata
     9001 acaagtctag cttttatatc gataacttgg tcagtcaaca aatcacgcgc acgtactcct
     9061 ataatttgat tgttgtcatc aaataagaag tcctcagctt tgacatgact agcaatataa
     9121 gctccatccc tattagcacg tttaatattt tcaatcacta gacgtgcatc gttattacgg
     9181 aaatctaaat agatccctcc gcctagcaaa ccttcttttt gtaaatcagg ctcacgctta
     9241 agaacatctt ctgcgctcaa aactttattg gctgcgggtg tatttgtcac acctgctaat
     9301 aaatcatata aatccatagc cactttaagg cgaaacatac taaaagtaga acccggctca
     9361 tcgtaaaccg gtaaaagcat tggatctggc ttaggaatgt gtggtgcaat ttgctgcacg
     9421 actgcacgct ctgatactgt atcagaaaca acttctacat caaattgctt caagtaacga
     9481 agaccaccat ggaccaattt tgttgagcga ctacttgtcc cttctgcaaa atcttgcatt
     9541 tcaataagtc cagtatctaa tccactagct gctgcttgta aagcgacacc agcgcctgtg
     9601 atacccccac ctatgataag taaatccaga gtacgatctt gcattctttg taaggctaaa
     9661 cgtcgtgttt ctcgtgaaaa ttccatctct acaccccctt aatcctcttg agcaaatact
     9721 tgtgtcgctt tgacagcttt tctccaccct ttatacagtt tttcttttcg agactcattc
     9781 attgttgctt ggaaaagttg tcctgttgcg tttagttctt ttagttcatc catactttcc
     9841 caatagccaa ctgaaagtcc ggctaagaaa gctgcaccca aggcagtagt ttcaaggttc
     9901 ttcgcacgag caatatcaat ccctaaaata tccgcttgga attgcattaa taaattattc
     9961 attgcagctc caccatctac acgaagttgt tggatatcaa ttcctgaatc aacttgcata
    10021 gtgtcaataa cgtctcgtac ttgataagca attgactgaa gtgtagcttt aacaaaatct
    10081 tctttacttg tcccacgagt taatccaaat actgaccctc tagcatttga atcccaatac
    10141 ggtgcaccca aacctgtaaa agctggaacc acataaacct catcgtcact tgttgatgat
    10201 tgagcaaggc cttctgactc acttgacgtt tcaatcattc taagaccatc acgtaaccat
    10261 tgaatagcgc ttcctgcaat gaagattgat ccttcaagtg cataatgaac cttaccatta
    10321 atgccgtaac caatcgttgt taatagatta ttttgagaca gttgcatctc ttcgcccgta
    10381 ttcatgataa tgaaggaacc tgtaccataa gtgtttttta ccataccagg ttcaaaggcc
    10441 aactgtccaa ataatgctgc ctgttggtca ccagccatac cagaaattgg cacctctcca
    10501 ccgtaaaaat ggaatggagt agttttccca taaacttcag aattagattt tacttctggt
    10561 aacattgctt taggaatatt cagtaactct aaaatttcat catcccattt aagttcttta
    10621 atattataaa gcatcgtacg agcagcattt gaatagtctg taacatggac gagaccatca
    10681 gttaacttcc aaactaacca agtatcaatt gttccaaaaa gcaactctcc tttttcagca
    10741 cgttcctgtg ctccaggaac atggtctaaa atccaacgaa cttttgttgc cgagaagtaa
    10801 gcatcaatga caagacctgt tttctcatga atcatattag tatgaccttc ttgcttcaat
    10861 tgatcagcaa tcggggctgt ttgtcgcgat tgccatacaa tagcattata aattggaaga
    10921 cctgtttttt tatcccaaac aacagttgtt tcacgttgat tggtaatccc aatagcctct
    10981 atctgtccag gtttgatgct tgattcaata aaagctccag caatgacaga ttgaacagaa
    11041 ttccaaatct gattagcatt atgctctacc cagcctgctt gaggaaaaat ttggggaaat
    11101 tctttttggc tactcgcaat tttttcacct tttttattaa aaataattgc tcgagaactt
    11161 gttgttccct ggtcaatcgc cataatgtat ttttcttctg aagacataaa cttcctcctt
    11221 aaaagtaagc gttttctttt gtaattctat tatacctaaa tcagtctatc ctgcactatc
    11281 aaaaatttaa tatgccccta aaatttttga taagatatca tcaataacac caaattagcc
    11341 aaataatagc taaatcaata atcattccga tataccaaac tagtctgaaa taccatttag
    11401 tcgttttatg acgaaaaact tctccaccta ggaatgcacc aaatcctcct aataataaac
    11461 tctctattaa caatgttttc tcactaatac gccatgcccc tctaattgca cgccttttat
    11521 caatagcata ggttgcaaat actaaaatat tccataacag taatgctaat agtaattcaa
    11581 tcattatttt ttccttccta ataaaaaagg cgagacaaag attatctttt gccccaccca
    11641 agtgttgact ttaatctaaa attgtctccc cattaagaat ttggatcatc caaactacga
    11701 ccatgtagtc ctttttctct ctggacttga cgaagttttt ccggggtaac atcattgccc
    11761 tcatcatcca ctaatttaat tccttcaacg tggtgacgga ctgatcggcg gaaaccttca
    11821 atatattctt cacggagctt tgcttgttca accttctctt ctccggtcaa tccaactgtt
    11881 tttttctttt ttgaaagctc gttaatacga gcaatttttt taggatccat aaaatgcctc
    11941 cttattttgt gtttaataag ttaaaagcag cgactgttct tgcttttgca acaaggctat
    12001 tgagtaaagc cagacgattt tgtttcattt tttcatcatc tgtcattacc attgtattat
    12061 caaagaaatc attaataatt ggactaagtg cgaaaagctt atctaagttg tcatgcatat
    12121 cctcagtcag ttcaagtgac aaaatagctt gatatagcgc cttctcctga ttattttcaa
    12181 acaatgaata gtccaccgat acagaatgag taactttttc agctaaatta aatgcacgtg
    12241 acaaactttc aacggaaact ttatattgag catccttgct cttttgcacc aatgcttggc
    12301 tagcttctag aataaggcta acaatataag tgtctgattc taaaacggct tctcgaatat
    12361 ctttagggac cttagagcca atcatctttt caatacgagc tgaaataaag gccatgacct
    12421 ctttttgatt agcataatct aaactagcaa acgatagacc ataaaggtta gttactaatt
    12481 catcaagtgg gatatcccaa ccaaaagctt ccaaaatacg gacaatcccc tgagtagctc
    12541 gacgaagagc atatggatca tttgaaccgc ttggaatcaa accaactgag aagaatgaaa
    12601 gtaaggtgtc aaatttatca gctaacgcta gaattgcacc aactctggtt tctggaagct
    12661 ctccatcggc cgatgtcggc atataatgtt cacgtatcgc tgctgctact gcgggctgtt
    12721 cccctgcaag taaggcatat ttctcaccca taataccttg taattcatca aactcatcaa
    12781 ccattccagt taagaggtca aatttataaa tacttgcagc tcgaagcaca gctaattttt
    12841 cttcatcaga aagatctgcc ttctcagcca aatattgtga aataactttt acacgatcca
    12901 tatgttcata aagtgaacca attttttcat ggaaagttac ttgttttaat ttctcaacta
    12961 aatctgcaat attaagtttc tgatcttctt gccagaagaa ttcaccatct tcgagacgag
    13021 ccactaaaac tttttcatta ccttttataa cattttcgat atgctcagca tttccattac
    13081 gcacagaaat aaagtttggt aacaattttc catccctgtc gcgaacaaca aaatagcgtt
    13141 gatgattttt cattgaagtg actaaaactt cttcaggtac atcaaggtat ttctcgtcaa
    13201 aagaacccaa gaatgctgtc ggatactcaa ctaaattaag cacttcattt agtaaatctt
    13261 cgtcaatttc aactgaaata ttttcctttt cttcgatagc atgaatttga tcaacgatca
    13321 tttgctggcg ttcttttgca tcagcaataa caaattgttg tcgcaagtca tcttcatacg
    13381 aactagcaga taaaatttca gtgtcagaac ccaagaaacg gtgccctcga ctaatacgcc
    13441 ctgaatgaat atcaaggaag tctagctcta aggcttgatc atctaataat accactaaag
    13501 tgtgaactgg acgaatatat tcaaaactat tatttgccca atgcatgttc actgggaaag
    13561 tcaattctgt taatacctct gtcacacttg ctaaaacatc aatcgcactt tttccagttt
    13621 catgcttagt gacataaaca tattcttctc ccttaacttc acgaaattcg atatcatcaa
    13681 cacttaaacc ttttccgcga acaaaacctt gggctgcttt agaaaaattc ccttctgcat
    13741 ccaaagcaat tttcttagaa ggacccttga aatcttctgt taaatctgtc tgctggtctg
    13801 ccaatccttt aacacgaaca gccaaacgac gtggagttga aaaagtttga acagtttcaa
    13861 atgataaacg atgatcctct aaaaacttga ccattttttg gcccaattgc ttctcactcg
    13921 gtgtcacaac ataagctggc aactcttcaa ggccaagctc caataataaa tctttagtca
    13981 tttcctactc cttactgtaa aacgcttcaa atgcttctct taaatcctga ttcatcttat
    14041 caagaacaac ctgcctatct aaaatatcgg taccttctgc acgaacaaca cagaaatcat
    14101 caaaagccat aaaatcttta aagatagctt ttaaataatg aacaggaaat tcatatggac
    14161 tatattgtcc tttagaataa atagaacccg cagattctaa cataagcaga cgatagtcat
    14221 ctgtcattaa tccgaccgaa ccattttcag tgtacttgaa agtctcacct gcaataaaaa
    14281 tattatcaat ataagttttt gctctagcag tcacattgta attgtgcatt ggagaaacaa
    14341 agacaatacg gtgatgtgac ttaaactgtt caagtaaatc tttagagatt ttagcttgaa
    14401 caatctcatc ggctgttaat tccaaatcct ttctttgttt agaccaaatc gaaagtagta
    14461 cttctttcgt aatttctggc aaaacacaat catataaatt taaaatagat aactgatggt
    14521 taggaaaatg ctcattataa agttcaataa acttttcttg tagaatagtt gtaaatgagt
    14581 acggattcga aaaatcagga tgactattaa caatgagtgt gttcattagt cttcctcagc
    14641 aagtaatttg atacgagttt cctcatccaa aagtgggaaa ccaagttttt tacgttcggc
    14701 aacaaaggtt ttagcaacaa cacgtgctaa attccggata cgtgcaatat agcctgcacg
    14761 ctcagtaaca gatactgctc cacgtgcatc taataaatta aaggtgtgtg aacatttaag
    14821 gacgtagtca taagctggat gaacaagtcc ttcttctaaa gcacgttttg cttctctttc
    14881 aaacttctca aagttttcaa gtaacattac ttggtctgat atctcaaatg aatattttga
    14941 atgctcatac tctggctgag taaagatttc accatattta acacctggtg cccactcaat
    15001 atcataaaca gaatctacct cttgaatata agaggctaaa cgctctagtc cataagtaac
    15061 ttcagaagtt acaggacctg tttgaagtcc acctacttgt tggaaataag taaattgtgt
    15121 tatttccatt ccatcaagcc aaacttccca acctaatcca gctgaacctg tagatggatt
    15181 ttcccagtta tcttcaacaa aacggatatc atgctcaagt ggattgatac ctagtaattc
    15241 caaagacttc aaataaagtt cttgaacatt tgacggcgaa ggcttcatta ccacttgaaa
    15301 ttgatgatgt tggtaaagac ggtttggatt ttcaccatag cggccgtcag ctggacgacg
    15361 tgatggttca acgtatgcag cattccaagg ctcagggcct atagcacgta agaaagtata
    15421 agggctcatt gtccctgctc ctttctcatt atcataagct tgcattaaca tacagccttg
    15481 gtcattccaa aactgttgta aagttagaat aatttcttga aaagttaatt tcttagacat
    15541 cattttcctt ctttcatact ttattacagc tgttgggaag acgacaacaa ttgactcaaa
    15601 acggataaat gataaaaacc acgtcctaca ccacctatgc ccaacagcac aggggcgtca
    15661 aagacgcggt tccaccctaa tttattactt catttttgat tgtactttta ctcactaaaa
    15721 agtgccaata ttcatcctat tctacccaac tttcactacc ttgagctcgc taaaaaaaca
    15781 gctgatgaac acctcttctt actgagacag tataacaaaa aatgactttt atgtctacta
    15841 tttatcttat tcttaagata ccctagactt tttgattcca tttggaataa tgat
//
LOCUS       DATRTN010000038        14881 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.038, whole genome shotgun sequence.
ACCESSION   DATRTN010000038 DATRTN010000000
VERSION     DATRTN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14881
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.038"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..383)
                     /locus_tag="VBE22_001174"
     CDS             complement(<1..383)
                     /locus_tag="VBE22_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202641.1"
                     /translation="MLIKGLLSPNSKVSPKDYKVYMRVLSFVFSVCSLIMIVSSFLHT
                     FESNFTKGFSFGSGIALFAGSFYFLVITFSEKRFQHYYTKVFDERNQFIANMSCRLLF
                     LLSVLVLVVLCLINVWFGITFSYAK"
     gene            complement(424..561)
                     /locus_tag="VBE22_001175"
     CDS             complement(424..561)
                     /locus_tag="VBE22_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202642.1"
                     /translation="MSRMWTIDFCPPNVYQQYVFIITISISIAMSIFAIYRYASLKKT
                     I"
     gene            complement(620..826)
                     /locus_tag="VBE22_001176"
     CDS             complement(620..826)
                     /locus_tag="VBE22_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7202643.1"
                     /translation="MKNSLQKLRKSRKLSQAELAVALGVTRQTIISLEKEKYTASLEL
                     AFKIARYFDKQIEEVFIYTESERG"
     gene            complement(977..2170)
                     /locus_tag="VBE22_001177"
     CDS             complement(977..2170)
                     /locus_tag="VBE22_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEO7202644.1"
                     /translation="MSKTIAINAGSSSLKWQLYEMPEEKVVAKGIIERIGLKDSISTV
                     KFDDKKDEQILDIVDHTQAVKILLEDLTKHGIIKDFNEITGVGHRVVAGGEYFKESAL
                     VDDKVVEQVEELSALAPLHNPAAAAGIRAFREILPDITSVCVFDTAFHTTMQPHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHQYVAQEAAKQLGRPLEELKLITAHVGNGVSITANYH
                     GQSIDTSMGFTPLAGPMMGTRSGDIDPAIIPYLVANDSELEDAAAVVNMLNKQSGLLG
                     VSGTSSDMRDIEAGLQSKDPNAVLAYNVFIDRIKKFIGQYLAVLNGADAIIFTAGMGE
                     NAPLMRQDVIAGLSWFGIELDPEKNVFGYFGDITKPDSKVKVLVIPTDEELMIARDVE
                     RLKAK"
     gene            complement(2202..3176)
                     /locus_tag="VBE22_001178"
     CDS             complement(2202..3176)
                     /locus_tag="VBE22_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7202645.1"
                     /translation="MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSC
                     DLDIVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQTEQLQANHQFTPDSIGFILLFLL
                     EELTSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
                     AQFIQEDAVRPQILKESDVIISDLPIGYYPNDDIAKRYAVSSSKEHTYAHHLLMEQSL
                     KYLKKDGIAIFLAPENLLTSPQSDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFV
                     LKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSKNMIE"
     gene            complement(3291..3662)
                     /locus_tag="VBE22_001179"
     CDS             complement(3291..3662)
                     /locus_tag="VBE22_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGG"
                     /protein_id="HEO7202646.1"
                     /translation="MILKKKLKAGILLQAIVLAAVFTLVLQFYLARILATERQYHSQI
                     EASKAYLTAQLAYKTIEGDSISGKCYFTGGYASYLQEGNYLQVKVTLDKGGNYNHKFY
                     RKTDTLQVPLKSNDNKASHKH"
     gene            complement(3640..4101)
                     /locus_tag="VBE22_001180"
     CDS             complement(3640..4101)
                     /locus_tag="VBE22_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001880689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEO7202647.1"
                     /translation="MKVIRKFCTYQKLRVKAFTLLECLVALVTITGALLVYQGLTKLL
                     AQQIVVMSSSSQSEWVLLTQQLNAEFEGAHLEYLRQNKLYLRKQDKIVTFGKSNKDDF
                     RKTGYDGRGYQPMVYGLDNCQMSQTKSMVKLVFYFKDGLKRTFYYDFKEET"
     gene            complement(4055..4354)
                     /locus_tag="VBE22_001181"
     CDS             complement(4055..4354)
                     /locus_tag="VBE22_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type II secretory pathway, pseudopilin PulG"
                     /protein_id="HEO7202648.1"
                     /translation="MVIIVRKKIRAYILLESLVGLAILVTITSLVISEINRQQSHIAR
                     RQHEQEVLILARMAVQTDQKILEANGVNIRIEESKNKIVLFEGNKEILHVSKVKG"
     gene            complement(4326..4754)
                     /locus_tag="VBE22_001182"
     CDS             complement(4326..4754)
                     /locus_tag="VBE22_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEO7202649.1"
                     /translation="MKNLLLKCKDKKVKAFTLLESLIVLSVVAFMTLVFSTSFNNIFR
                     QVEETIFFISFEHLYRDTQKLSAFGQKKQTLTISHNYLENTYERLYLPKTVKVVKSDT
                     LAFDANGGNSSLAKIQFECYRKTVTYQLYIGSGNYRKKEN"
     gene            complement(4751..5842)
                     /locus_tag="VBE22_001183"
     CDS             complement(4751..5842)
                     /locus_tag="VBE22_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEO7202650.1"
                     /translation="MTLRQVTLKNTHQTSGIDKWISWLKKDISVRNRHKSKKLSLKKQ
                     RKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDYYTDRMNKALLEGKDLSKMLGELGFS
                     DTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLTFLVLIMIGL
                     RHYLMPQLGENNFATSLITNVPNIFLLLLAVVLIFSLIFYIVQKRLSRIKVACFLTTI
                     PLVGSYVKLYLTAYYAREWGNLLSQGVELDQIVKVMQNQKSKLFREIGYDMEEGFLSG
                     KAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQP
                     VIFIFVALIIVMIYAAMLLPMYQNMEILS"
     gene            complement(5727..6659)
                     /gene="tadA"
                     /locus_tag="VBE22_001184"
                     /pseudo
     CDS             complement(5727..6659)
                     /gene="tadA"
                     /locus_tag="VBE22_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            complement(6832..7197)
                     /locus_tag="VBE22_001185"
     CDS             complement(6832..7197)
                     /locus_tag="VBE22_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEO7202651.1"
                     /translation="MYQVVKMFGDWEPWWFIEGWEEDITEIAEYDTLSEALLYFQEEW
                     DRGQEKWPYFQSKSSLLATFWSIKEKRWCEECDEYLQQYHSLMLLKEWQEIPKEESIE
                     RFEVFNKIAELPSACSLNL"
     gene            complement(7311..10961)
                     /gene="rpoC"
                     /locus_tag="VBE22_001186"
     CDS             complement(7311..10961)
                     /gene="rpoC"
                     /locus_tag="VBE22_001186"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEO7202652.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIICDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPMDTPLEPKSLLTEREYREKLQ
                     EYGYGSFVAKMGAEAIQDLLKRVDLDAEIAVLKEELKSATGQKRVKAVRRLDVLDAFK
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDLAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDHDEAVMAYQNGYVHLHTRVGIAVDSMPNKPWTEEQKHKIMVTTVGKI
                     LFNDIMPEDLPYLIEPNNANLTEKTPDKYFLEPGQDIQAVIDNLEINIPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     DINKAFRRGLMTEEDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLTITAITDGKEVTETLEERLIGRYTKKSIKHP
                     ETGEILVGADTLITEDMAAKVVKAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGEVVAIEEDSSTRTKKVFVKGQTGEGEYVVPFTARMKVEVGDEVARGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPLAVNEVEIIEGTPVDA
                     EVTEVSTPTTED"
     gene            complement(11078..14653)
                     /gene="rpoB"
                     /locus_tag="VBE22_001187"
     CDS             complement(11078..14653)
                     /gene="rpoB"
                     /locus_tag="VBE22_001187"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO7202653.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDAG
                     LKEVFEDVLPISNFTDTMDLEFVGYELKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDAKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGDSELVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNLKTRLLNQTIAENLVDGETGEILVEAGTVMTRDVIDSIAEHIDGDLNKF
                     VYTPNDYAVVTEPVILQKFKVVAPTDPDRVVTIVGNSNPEDKVRALTPADILAEMSYF
                     LNLAEGIGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRSTGAVTNEIV
                     WLTADEEDEFTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFPSSIVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAKH
                     DGRVIFSDAEKVEVRREDGSLDVYHVQKFRRSNSGTAYNQRTLVKVGDLVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEDSLRDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGVVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWETVQEAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDVMHVDDLEKARVKQETEEKQAEQVSEVVQED"
BASE COUNT         4639 a         3075 c         2409 g         4758 t
ORIGIN      
        1 aactttgcat aggaaaaagt tataccaaac cagacattaa tcagacacaa taccactaaa
       61 acaaggacag ataaaaggaa aaggagtcga caagacatat tagcaataaa ttgattacgc
      121 tcatcaaaaa ctttggtata gtaatgttga aatcgctttt cactaaatgt tatcaccaaa
      181 aaataaaaac ttcctgcaaa taaggcaata cctgaaccaa aggaaaaacc tttagtaaaa
      241 ttagattcaa aggtatgtag aaatgatgat actatcataa tcaaactaca tacactaaaa
      301 acaaaagaca aaactctcat atatactttg taatctttcg gagatacttt actattgggt
      361 gacaataatc cttttattaa catagaatac tactttctaa aacttcaatt taatcatcag
      421 ctattatatc gttttcttca atgaagcata gcgataaata gcaaaaatag acatcgcaat
      481 actaatacta atagtaatga taaaaacata ttgctggtaa acgtttggtg gacaaaaatc
      541 aatagtccac atccttgaca tattacagat taacttttaa ccttttgtct tttgtggact
      601 gtatttttcc attaaaatcc tatcctcttt cactttctgt ataaataaaa acttcctcaa
      661 tttgtttatc aaaataacgc gctatcttaa atgctagctc caaagatgcc gtgtatttct
      721 ctttttctag agaaataata gtttgcctag taactccaag tgcaactgca agttctgctt
      781 gacttaattt cctagatttt cgtaactttt gtagagaatt tttcatatct tacacctttt
      841 tgtatagtta actttacatt tatagtatag ttaactatac attttttgtc aagtacattt
      901 tacatacctg ttcaaaaaac aaaaaagttg atagttaaac tatcaacttt tctggtctta
      961 tcaatttttt gataacttat ttagctttaa ggcgctcaac gtcacgcgca atcattaatt
     1021 cttcatcagt aggaataact aaaaccttca cttttgaatc tggttttgtg atatctccaa
     1081 aataaccaaa aacatttttt tctggatcaa gttcaatgcc aaaccatgat aatcctgcga
     1141 taacatcttg acgcattaat ggcgcatttt ctcccatacc tgcagtaaaa ataatagcat
     1201 ccgcgccatt taatactgcc aagtattgtc caataaattt cttaatacgg tcaataaaga
     1261 cattataggc taacactgca tttggatctt tactttgaag acctgcttca atatctcgca
     1321 tatcacttga agttcctgat actccaagca atcctgattg tttgttaagc atattaacaa
     1381 cagctgctgc atcttccaat tctgaatcat tagctacaag ataagggata atcgcaggat
     1441 caatgtctcc tgaacgtgtt cccatcattg gtcctgcaag tggtgtgaat cccattgatg
     1501 tatcaataga ctgtccatga taatttgctg taatagaaac accattaccc acatgtgctg
     1561 tgattaattt taattcttct aaaggacgtc ctaattgctt agctgcttct tgtgcaacgt
     1621 attggtggct tgtcccgtgt gcaccgtatt tacgtacttt ataatcagta tagtactttt
     1681 gtgggattgg atataagtag gtatgaggtt gcattgttgt atggaaggct gtatcaaata
     1741 cacatacact cgtgatatct ggtaagattt ctctaaacgc acgaatccct gctgcagctg
     1801 ctgggttgtg gagtggagct aaagctgata attcttcaac ctgttctaca actttatcat
     1861 ctactaaagc agactcttta aaatattcac caccagcaac gacacgatga ccaacacctg
     1921 taatttcatt aaagtctttg ataataccat gttttgttag atcttctaaa agaattttaa
     1981 cagcttgggt atgatctacg atatctaaaa tttgttcatc ttttttatcg tcaaatttta
     2041 cagtcgaaat agaatctttc aaaccaatac gttcgataat ccctttggca actactttct
     2101 cctctggcat ttcatataat tgccatttca aacttgaact tccagcatta attgctattg
     2161 tttttgacat aatttacctc tctaaagcgt tttcacaatc tctattctat catatttttt
     2221 gagtaatgac tattttctct gctccatttt tgaaaatttt caatgaagtt tgccatattc
     2281 tcacgatttt gcaaatctgt cagcggatat acaaaggttt ctggtttttg ttctgcttgc
     2341 ttcttgagaa caaatataga tttcgcattt tgacgacttc caaaaatagt ttctggtaga
     2401 gttaaaacgg caatgacatc tgcatatcct tttaaccact ccttcagcaa atcactttgt
     2461 ggacttgtta aaaggttttc gggtgctaaa aatatagcga ttccatcttt tttcaaatat
     2521 ttaagagatt gctccatcaa tagatggtga gcataggtgt gctctttaga acttgataca
     2581 gcatatcgtt tagcaatatc atcattagga taatagccaa ttggtaaatc actaatgatt
     2641 acatcgcttt ctttcaaaat ttgtggtcta acggcatcct cttggataaa ttgggcacta
     2701 gaacctataa tttcagcaat gcttgctgat agatcaatca aaagatcatc aacttcaatg
     2761 cccatataat ttaactcttt cgagctgtta ttgaggagag tctgagctaa attcccagtt
     2821 ccacttccaa tttccaagac atccactgtc tcttgactcg ttaattcttc caaaagaaat
     2881 aacaagataa aaccaatact atctggcgta aactgatgat tagcttgtaa ttgctctgtt
     2941 tgcgcagatt tgataaaaat gaactggaaa gtgcgacgcc attcttcttg acttaagtca
     3001 agttgacgta atttttggtt attcaccaca acaatatcta aatcacaact tgaaccaagg
     3061 taataagagt tctgttcaat taaggcatca taaatatgag tttttaattg gttctcaatc
     3121 gtttggatat tttctaaaat cagctcatag gctgtctcaa ttttttcaaa attcatgatt
     3181 tcctccagaa tttatcataa caaaaaagac agcatctgaa aagagaagca aacaaaaatc
     3241 ctgtttttaa gcaaaagctt gtcttactaa tatttcatca ctgtatattt ttaatgttta
     3301 tggcttgctt tattatcatt agacttgaga ggtacttgga gagtgtctgt tttacgataa
     3361 aacttatggt tatagtttcc tcccttatcc aatgtaacct taacttgtaa atagttccct
     3421 tcttggaggt atgaagcata tcctcctgta aaataacatt ttcctgatat ggagtcacct
     3481 tctattgttt tataagctaa ctgcgctgtt aaataagctt tacttgcttc aatttggcta
     3541 tgatactgtc tttctgttgc caatattctt gctagataaa actgtaagac gagtgtaaat
     3601 acagcagcca atacaatagc ttgcaaaagg atgccagctt taagtttctt ctttaaaatc
     3661 atagtaaaat gtccttttta acccgtcctt aaaataaaaa acaagtttta ccatactttt
     3721 ggtttgactc atttgacaat tgtctaaccc ataaaccatt ggttgataac ctcgaccatc
     3781 ataacctgtc ttacggaaat catctttatt agatttgcca aaggttacaa tcttatcttg
     3841 cttacgtaaa taaagtttgt tctgtcttaa atattccaag tgagcgcctt cgaattctgc
     3901 atttagttgc tgagttaata acacccattc agactgactg gaagaagaca tcactactat
     3961 ctgttgagcc aacaattttg tcagtccttg ataaactagt aaagctcctg tgattgtaac
     4021 caatgctacc aaacattcta aaagcgtaaa tgctttaacc cttaactttt gatacgtgca
     4081 aaatttcctt attaccttca aagagcacaa ttttattttt actttcttca atgcgaatat
     4141 taacaccatt tgcttctaat attttttgat cagtttgcac agccattcta gctaatatca
     4201 atacttcttg ctcatgttgc cttcttgcaa tatgagattg ttgacgattg atttcactta
     4261 ttactaaact tgtaatagta actaaaatag ctaatcccac taggctttcc agtaaaatat
     4321 aggctctaat tttctttctt acgataatta ccacttccta tatataattg atacgtaaca
     4381 gtttttctat aacattcaaa ttgaattttt gccaagcttg aattccctcc attagcgtca
     4441 aatgcaagtg tgtcactttt gactactttt acagttttag gtaaataaag tctctcataa
     4501 gtattttcga gataattatg agagattgta agggtttgtt tcttctgacc aaatgcactc
     4561 aatttctgag tatctctata aagatgttca aaggatatga agaaaattgt ttcttcaacc
     4621 tgcctaaaaa tattattaaa tgatgttgaa aatactaacg tcataaatgc cactactgat
     4681 aatacaataa ggctctctaa aagtgtaaat gctttaacct tcttatcctt acattttaac
     4741 aataaatttt tcatgataat atctccatat tttgatacat tggtaacagc attgctgcat
     4801 aaatcataac aatgataaga gctacaaaaa taaaaataac aggttggatt aactgggtcg
     4861 ctctagctaa ttttgtaaaa aaatcctccc acttctcatc agcatatata tctaactctg
     4921 ttcctaattt cgccttaact tggccatatt caatcattaa actaagctca gttaagaaaa
     4981 acggatagtc taatactttt tggtgaaatg ctttacctga tagaaaacct tcttccatgt
     5041 catatcctat ttccctaaaa agtttggatt tctgattttg cattacttta acaatttggt
     5101 ccaattcaac accttgactt aataaatttc cccactcacg ggcatagtaa gcagttaaat
     5161 aaagcttaac atatgatcca actaagggaa ttgttgttaa aaaacaagct actttaatac
     5221 gcgacaatcg cttttgaaca atataaaata ttaaactaaa aataagtaca actgctaaaa
     5281 gtaataagaa aatattcggc acatttgtaa tcaggctagt tgcaaaatta ttttctccta
     5341 attggggcat taaataatgc ctaagaccaa tcataattag caccagaaaa gtcaataata
     5401 tcaatgggta agtagctact tctattactt tttttctaac taacaaaaga ttagctaaat
     5461 aagactcaat ctttagtagg ctccttgaaa tgttaccatg caaatcagct aatgcaacct
     5521 gtgtgataac agtgtcagaa aaacctaact ctcctaacat ttttgataaa tcttttccct
     5581 ctaataatgc cttattcatc ctatctgtat aataatcaga caataactta ctcctcttta
     5641 agaaggtgac catatcagtt aaagaaaaac cactagcaaa aagattatta aaaagttgga
     5701 ctactttccg ttgtttcttg agggataatt ttttcgactt gtgcctgttt cttactgata
     5761 tgtccttctt cagccaagat atccacttgt ctattccact tgtctgatga gtgtttttta
     5821 aagttacctg tctcaaagtc aattaggctt cctcctccaa ttaaacgttg atatgctatt
     5881 aattttagac tattttctaa ctcttgatag ttaaccccta attctataag cctatcatag
     5941 actccggaaa tacttttagc atgaatagta gaaaaaacca tcactcccgt taaacttgca
     6001 cgaataacag cacgggccgt cgcttgatct ctaatctctc cgataattaa aatatctgga
     6061 cgatgccgta aagacagttt gattaaagca tcataagtca ttccaatatc ctcattcaat
     6121 tggagttgta acatcttgtc attcttgatt tctaccggat cttcaatcgt gataatttgc
     6181 ttatttttaa atacttctga agctaattga tacatgagag ttgttttacc actccccaca
     6241 gggccggaaa aaagatatag ccctcttgta cccagtactt ccttcatttg ctttatatta
     6301 tcaaaccaat attttaagtc ctgatgacct gaatacaaaa tacgaataac taaagattct
     6361 tgaccacgat aatctcccac actcgatagt cgtaatgaaa ccagtcttcc ctctgacagt
     6421 tcatagtcac aagaacctaa ttgacttcgt cttttttctc caacgttcat gcctgccaca
     6481 aatttaaagt gactaataag actagccatc ctattaaact caaaaacatc aataaaccgc
     6541 ctttcatcat caatacgcat atagagttca taacaatcac ctttgggaat gatataaata
     6601 tcttgagcat ttacttctac tgcctgatga atgacttgct ttgctaatga ttgaaccatc
     6661 ccgtacctcc ttaaccatat attcgaaaat aaaataaaaa aatctcacca attggtgaga
     6721 ttttttcaat gatttcttga gaggtagtaa cataaatatt atgaagcaga ggctgaacaa
     6781 acctattata accccataaa aactatttaa ctttttttaa actactttta attacaaatt
     6841 taaagaacat gccgatggca actcagctat cttattaaat acttcaaatc gctcaataga
     6901 ttcttcctta ggaatttctt gccattcttt caataacatt aaggaatgat actgttggag
     6961 gtactcatca cattcttcac accaacgttt ttcttttata ctccaaaacg ttgccaacaa
     7021 actagactta ctttgaaaat aaggccactt ttcctgtccc ctatcccact cctcttgaaa
     7081 atagagcaaa gcttctgata aggtatcata ctcagcaatt tcagtaatat cttcctccca
     7141 accttcaata aaccaccatg gttcccaatc accaaacatt tttacaactt gatacataat
     7201 acacttcctt tagagagtat tatataaaaa ataagaataa aataaaaaat atctgctcat
     7261 tccaaattct tgtaaaaaaa gaacctgagt taaactcaag ttctttttga ttaatcttct
     7321 gttgtaggtg ttgatacttc cgtaacttct gcatcaacag gagttccttc aataatctca
     7381 acttcattaa cagcaagtgg ttcaatatta cggtaacgag ccataccagt acccgctgga
     7441 atgattttac cgataataac attttcttta agaccaagta agtgatcttt cttaccacgg
     7501 atagcagcat ctgtaaggac acgtgttgtt tcttggaagg atgcagctga taagaatgaa
     7561 ttagtttcaa gggaagcctt agtaataccc ataaggacag gacggcttgt cgcagggatt
     7621 ccaccagaaa taacaatatc tttgttagca tctgtaaagt ctgaaatatc cataagtgta
     7681 cctggaagaa gatctgtatc tcctggatcc atgacacgaa ctttacggag catttgacga
     7741 accattacct caacgtgttt atctccgatt tctacccctt ggctacggta aactttttgt
     7801 acttcagcaa gtaggtacgt ttcaacagat aaagtatcac gaacttcaag gagacgtttt
     7861 ggttggatag atccttcagt taaggccgca ccacgagcta cttcatcacc aacttcaact
     7921 ttcatacgtg ctgtaaatgg aacaacgtat tctccctcac ctgtctgacc tttaacgaat
     7981 actttcttag tacgtgttga agagtcttct tcaattgcaa cgacttcacc cttaacttca
     8041 gtaataactg cctcaccttt agggttacgt gcttcaaaga tttcttggat acgtgggaga
     8101 ccttgtgtaa tatcagtatt tgaagcaaca ccaccggtat ggaaggtacg cattgtaagc
     8161 tgtgtccctg gttcaccgat agattgagct gcaatagtac caactgcttc accaacctca
     8221 acagcatcac ctgttgccaa gttaataccg taacagtgac gacaaacacc atggcgagtg
     8281 ttacatgtaa atactgatcg gatagttact tcctcaacgc ccgctttaac aactttagca
     8341 gccatatctt cagtaattaa ggtatctgca ccaacgagaa tttcacctgt ctcaggatgt
     8401 ttgattgact tcttagtata acgtcctatc aaacgctctt caagtgtctc agtaacctct
     8461 ttaccatctg taattgctgt gattgtcaaa ccgcggtctg ttccacagtc atcttcacga
     8521 ataataacat cttgggcaac gtcaaccaaa cgacgagtaa ggtaacctga gtcggctgtc
     8581 ttaagggccg tatcagtcat acctttacgc gcaccgtgag ttgagaagaa catttccaaa
     8641 acactaagac cttcacggaa gtttgataag attggcaatt ccatgatacg tccattagga
     8701 gcagccatca aaccacgcat accggcaagt tgtgagaagt ttgagatgtt accacgagct
     8761 cctgagtcca tcatcataac gatagggttc tttggatctt gtgtctcaat aagacgtttt
     8821 tcaagtgctt ctttagcttc acgccatgtt gttgtaacag caacataacg atcttcttca
     8881 gtcatcaaac ctcgacggaa ggctttattg atatcttcaa ctctgtggtg agctgcatca
     8941 ataatctcag ctttattgtc aataactggg atatcagcaa tacccacagt caaaccagca
     9001 agtgttgaat gataatatcc aaggtccttc aaacggtcaa ggaaagcaga agtttctgtt
     9061 gtacggaaac gtttaaaagt ttctgcgata atattaccaa gatttttctt cttaaaggga
     9121 atattaatct ctaagttatc aataactgct tgaatatcct gacctggctc taagaagtat
     9181 ttatctggag ttttttctgt caagtttgca ttattaggtt caattaagta cggtaaatct
     9241 tcaggcatga tatcgttgaa gagaatctta ccaactgttg taaccatgat tttatgtttt
     9301 tgctcttctg tccaaggctt attcggcatt gaatcaacag cgataccaac acgtgtatgt
     9361 aaatgaacat aaccattttg atatgccata actgcttcgt catgatcttt gaaaatcatg
     9421 ccttcacctt cacgaccagc atcttccata gtcaggtagt aatttcctaa aaccatgtcc
     9481 tgagatggtg ttacgactgg cttaccatct ttagggttaa ggatgtgctc tgcagcaagc
     9541 atcaaaagac gagcttctgc ttgggcttct tcagacaatg gaacgtggat ggccatttgg
     9601 tctccatcaa agtcagcatt gtaggcctca catacaagtg ggtgaagacg aagcgcctta
     9661 ccatcaataa ggacaggttc aaacgcttgg atacctaatc tgtgaagagt aggtgcacgg
     9721 tttagaagta ctgggtgctc tttaataact tcttctaaga tgtcccaaat acgctcgtcc
     9781 ccacgttcaa ccatacgttt agctgcttta acgttccctg cgaggtcacg agcaacgatt
     9841 tcacgcatta caaaaggttt gaagagctca atagccattt cacgtggtac accacattgg
     9901 tacattttaa gtgttggacc aacagcaata acggaacgtc cagagaagtc aacacgttta
     9961 ccaagtaagt tttggcggaa acgaccttgt ttacctttaa gcatatggct taatgatttc
    10021 aaaggacgac tacctggtcc ggtaattgga cgaccacgac gaccattgtc gataagcgca
    10081 tcaacagctt cttgtaacat acgtttttca ttttgaacga tgatgccagg agcatttaat
    10141 tccaacaaac gtgccaaacg attgttacgg ttaataacac ggcggtaaag gtcattcaag
    10201 tctgatgccg caaaacgacc accatctaat tgaaccatcg gacgaagatc cggtgggata
    10261 accggtaaaa tgttaagaac catccattct ggtttatttc cagatttttt aaaggcatct
    10321 aacacatcca gacgacgaac tgctttcaca cgtttttgac ctgtcgctga tttcaactcc
    10381 tctttaagaa ctgcgatttc tgcatctaaa tcaacgcgtt taagaagatc ttgaatggct
    10441 tcagcaccca ttttagcaac aaacgaaccg taaccgtact cttgaagttt ttcacggtat
    10501 tcacgctcag taaggagtga cttcggttct aatggtgtgt ccattggatc gataacaaca
    10561 taagccgcaa aataaataac ttcttcaagg gcacggggac tcatatccaa cgtgagtccc
    10621 atacgcgatg ggataccttt gaaataccag atatgagata ctggtgcttt taactcgata
    10681 tgtcccatac gttcgcgacg aacttttgca cgagttactt caacgccaca acggtcacaa
    10741 atgattcctt tgtaacgaat acgtttgtat tttccacacg cacattccca atcttttgtt
    10801 ggaccaaaaa taacttcatc aaaaagtcct tcacgttctg gtttcaatgt gcggtagttg
    10861 attgtttcag gttttttaac ttcaccataa gaccatgaac ggaccttact tggtgatgct
    10921 aatgtgattt gcatactttt aaaacgattt acgtcaacca ctagttttac ctttcttcga
    10981 tttatcatca tactagccta actaggattt caataaatcc taggactagc attgcatatc
    11041 aattctatat tcgctcctag ttataaacta gataaattta atcttcttga acgacttcag
    11101 atacttgttc agcttgtttt tcttcagttt cttgtttcac tcgcgctttt tcaaggtcat
    11161 caacgtgcat cacatcatca tcttcacctt catcaagatc acggagttca acttcgttgt
    11221 catcttcatc aaggacacgc atatcaagac caagtgattg caattctttt acaagaacac
    11281 ggaatgattc tggaacacct ggttttggaa ttggcttacc ttttgtgata gcttcataag
    11341 ctttaagacg tcctgtaaca tcatcagatt tgtatgttaa aatttcttga agaacatttg
    11401 aagcaccata agcttcaagc gcccaaacct ccatctcacc aaaacgttgt ccaccaaatt
    11461 gagctttacc tccaagtggt tgttgagtaa caagtgagta tggtcctact gaacgggcat
    11521 gaagtttatc atcaaccatg tggtgaagtt tgatcatgta catgacacca actgacacac
    11581 ggttatcgaa tggttcacca gtacgaccat cataaagaac agtcttagca tctgaatcca
    11641 tacctgcttc ttgaacagtt tcccaaaggt cttctgaaga agccccatca aagactggcg
    11701 ttgcaatatg aatacctaaa ttacgagcag ccatacctaa atgaagctcc ataacttgtc
    11761 cgatattcat acgtgatggc accccaagtg ggttcaacat gatatcaact ggcgttccat
    11821 ctggtaaata aggcatatct tcaacaggaa caatacgtga gacgacacct ttgtttccgt
    11881 gacgaccggc catcttatca ccgactttga ttttacgttt ttgagcgata taaacgcgga
    11941 caagcatatt aacacctgat tgcaattcat caccatttgc acgagtaaag attttaacgt
    12001 cacgaactac tccatcgcca ccgtgaggta cacgtagcga agtatcacga acttcacgtg
    12061 atttatcacc aaaaatggca tgcaagagac gttcttcagc agataagtcc ttttcaccct
    12121 taggcgttac cttaccaaca agaatgtcac cttcttttac ctcagcacca atgcggataa
    12181 ttcccatttc atcgagatca cgtagtgaat cttcaccaac atttggaatt tcacgagtaa
    12241 tttcttcagg cccaagcttt gtatcacgcg tttcagattc gaattcttcc aagtgaacag
    12301 atgtatagac atcttctttc acaaggcgct cactcatgat aacggcatcc tcgaagttat
    12361 aaccttccca agtcatataa gcaacaactg ggttttgtcc aagagccatt tcaccatttt
    12421 ccatagatgg tccgtcggcg ataaaatcac ctttttcaac gagatcacca actttaacta
    12481 aggtacgttg gttataagca gtacctgagt ttgaacggcg gaatttttga acatgataaa
    12541 catcaagaga accatcttcg cgacgcactt caactttttc agcatctgaa aaaataacac
    12601 gaccgtcatg tttagcaatc acagctgcac ctgaatcgtg ggctgcttga tactccatac
    12661 cagtaccaac atatggtgct tttggatcaa tcaatggcac agcctgacgt tgcatgttgg
    12721 cacccatcaa ggcacggtta gagtcatcat tttccaagaa aggaatacat gctgttgcga
    12781 cagcaactac ttgcttaggt gacacgtcaa caaagtcaac aatgcttgaa ggaaactctt
    12841 ggttattacc ttgatgacga cccataacga tttcttctgc aaatgtaccg tcttcgttaa
    12901 gcttagagtt ggcttgtgca actgtaaatt catcttcttc atcagcagtc aaccaaacaa
    12961 tttcgttcgt taccgcacca gtagaacgat ctactttacg atatggtgtt tgaataaatc
    13021 catatttatt gaggtgtcca aatgaagata agttattaat caaaccaatg tttggacctt
    13081 caggtgtttc aatcggacac atgcgcccat agtgggtata gtgcacatca cgaacttcat
    13141 atccagcacg gtcacgtgtc aaaccaccag gtcctaaggc agagagacgg cgtttgtgcg
    13201 acaattctga tagagggttg tgttggtcca tgaattgtga cagctgtgat gaaccaaaga
    13261 attctttaac agcggcagtc actggacgaa tgttaatgat ttgttgaggt gtcaagactt
    13321 cattgtcttg tacagacata cgttcgcgga cattgcgttc catacgagca agtccaatac
    13381 ggaattggtt cgcaagcaat tcaccaactg cacgaatacg acggttacct aaatggtcaa
    13441 tgtcatctac cttaccgata ccctctgcaa ggtttaagaa gtatgacatc tctgctaaaa
    13501 tatctgcagg agttaaggca cgaactttat cttcaggatt tgagtttcca acaatagtaa
    13561 caacacggtc tggatcagtt ggagcaacaa ctttgaattt ttgaagaata actggttcag
    13621 taactacagc ataatcattt ggtgtgtaga caaacttgtt taagtctcca tcgatatgct
    13681 ctgcaattga atcaatgaca tctcgtgtca taacagtgcc agcttcaaca agaatctctc
    13741 ctgtttcacc atcaactaaa ttttcagcga ttgtttggtt caataggcgt gttttaagat
    13801 tcagtttttt attaatctta taacgaccaa cagccgctaa gtcataacga cgtggatcaa
    13861 agaaacgtgc aactaaaaga ctacgtgagc tgtctgcagt ttttggttca cctggacgaa
    13921 gacgttcata gatttcttta agtgcttcat ctgtacgaga atcacttgga tttttatgaa
    13981 tgtctttttc aatagtattg cgaacaagtt cactgtcacc aaagatgtca acaatttcat
    14041 cgtctccaga gaaaccaagt gcacggacta gtgttgtgaa tggaatttta cgtgtgcggt
    14101 cgatacgtgt ataagcaata tcttttgcgt ctgtctcaag ttccaaccaa gctccacggt
    14161 tagggataac agttgaccca taaccaactt ttccattttt atctacctta tcattaaagt
    14221 aaacaccagg agaacggact aattgtgaaa caatgatacg ttcaccacca ttgatgatga
    14281 aagtacccat ttcagtcata attggaaaat caccaaagaa aacttcttga gttttaattt
    14341 caccagtttc tttattaact aaacggaaag taacaaaaat aggtgctgag tagctagcat
    14401 catggatacg agcttcttcc aaagtatatt taggttcttt taattcataa ccaacaaatt
    14461 caagatccat agtatctgta aagtttgaaa ttggaagtac atcctcaaat acttctttca
    14521 agccagcatc aagaaaatct tggaatgaat cagtttgaat ttcaattaag tttggtaaat
    14581 caagaacttc cttaattctt gaaaagctac gacgtgtacg gtgttttccg tactgaactt
    14641 catgtcctgc caagttttta gctccttttt aaagatagag tgactaagtt tttatctcat
    14701 aaaacttagt gagtttattt catttgctcg cctaacaagg acaagcttat agatgtcgtt
    14761 tgcggtaatt ttcagaaagt ttaaaaatgt attacaataa ccttttttct taaaattagg
    14821 cacaaaaaga gcagctaaat ctcactttat caagttgatt taactgctgt atatctaggt
    14881 c
//
LOCUS       DATRTN010000039        14832 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.039, whole genome shotgun sequence.
ACCESSION   DATRTN010000039 DATRTN010000000
VERSION     DATRTN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14832
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.039"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..289
                     /locus_tag="VBE22_001188"
     CDS             <1..289
                     /locus_tag="VBE22_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202654.1"
                     /translation="NTNEIDKLLIPYLDKKYGEVSSEVAKLLRQMRNKIGHGDFKGFN
                     EKAEKFAQKFMKHFHFDYTEYSRLNWVLLHTCCLLDDLLRITIFQQLKVTK"
     gene            360..755
                     /locus_tag="VBE22_001189"
     CDS             360..755
                     /locus_tag="VBE22_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7202655.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     IIFGSLPPLSLIDGALLKYLGIPVVLTWFMCQKTFDGKKPYGFLKSIITYALRPKITY
                     AGKQIRLNKEKMNESITIVRSEQLNGTPH"
     gene            739..3192
                     /locus_tag="VBE22_001190"
     CDS             739..3192
                     /locus_tag="VBE22_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7202656.1"
                     /translation="MAHPIKYIEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKVQVHD
                     NFRQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALVSM
                     IGDNQVDYRFFIGFKLLLNEQEVSVKNIGKDIQKAFAEFFHGVNHKLMGDFVSMPMSE
                     IERFSKMEQLLESKLSRRFHIRPLGKDDFGYIIAHLYGQTGIPFDDYDYHLPKRYEDK
                     KVLVKKYDIIKPTRCLIEEKQRYLRMEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPIDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELETNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMLGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQAIIVDPKAERGKWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSEQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGN
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEGEK"
     gene            3189..5216
                     /locus_tag="VBE22_001191"
     CDS             3189..5216
                     /locus_tag="VBE22_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202657.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNHYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKAIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLDIGTK
                     IVLPDTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDIETLGEDRVEKLLATSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFSLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            5213..6142
                     /locus_tag="VBE22_001192"
     CDS             5213..6142
                     /locus_tag="VBE22_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEO7202658.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSISQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDPIGYGDLSSRYWQDHLIGARRVIHN"
     gene            6159..7088
                     /locus_tag="VBE22_001193"
     CDS             6159..7088
                     /locus_tag="VBE22_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7202659.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            7333..7449
                     /locus_tag="VBE22_001194"
     CDS             7333..7449
                     /locus_tag="VBE22_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO7202660.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            7465..9384
                     /gene="tet(M)"
                     /locus_tag="VBE22_001195"
     CDS             7465..9384
                     /gene="tet(M)"
                     /locus_tag="VBE22_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO7202661.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            9482..9670
                     /locus_tag="VBE22_001196"
     CDS             9482..9670
                     /locus_tag="VBE22_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO7202662.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(9728..10081)
                     /locus_tag="VBE22_001197"
     CDS             complement(9728..10081)
                     /locus_tag="VBE22_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7202663.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARIKRGLTREQVGTIIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQIEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            10613..11035
                     /locus_tag="VBE22_001198"
     CDS             10613..11035
                     /locus_tag="VBE22_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7202664.1"
                     /translation="MKPSSFQETIENQFDYICKQAIEDERKDYFKHLSRLSKKEVSFS
                     EIDNYLVTSFSTVDTYVTDFQLFTLYGLRINVESDLLSEALRHLPENKRNIILLHYFM
                     DMSDVEIAELLNVNRSTVYRHRISGLAMIKEFMKECKE"
     gene            11032..11262
                     /locus_tag="VBE22_001199"
     CDS             11032..11262
                     /locus_tag="VBE22_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7202665.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLITKILAFEIK"
     gene            11758..11958
                     /locus_tag="VBE22_001200"
     CDS             11758..11958
                     /locus_tag="VBE22_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEO7202666.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHTYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            11985..13178
                     /locus_tag="VBE22_001201"
     CDS             11985..13178
                     /locus_tag="VBE22_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7202667.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFNHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSDYRFVEPKTKASVRHIVLDECTLNYLSEWKKAQQNLIQT
                     DFVMSYNGIPTQKHTLAHAIERYAKLASIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(13246..14808)
                     /gene="guaA"
                     /locus_tag="VBE22_001202"
     CDS             complement(13246..14808)
                     /gene="guaA"
                     /locus_tag="VBE22_001202"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEO7202668.1"
                     /translation="MTDISILNDIQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TADEIRDINPIGIVLSGGPNSVYADGAFGIDEEIFELGIPILGICYGMQLITHKLGGK
                     VLPAGEAGHREYGQSALRLRSESALFAGTPQEQLVLMSHGDAVTEIPEGFHLVGDSVD
                     CPFAAMENTEKQFYGIQFHPEVRHSVYGNDILKNFAVNICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMDMLG
                     GKFGLNIIRVDASKRFLDLLSGVEDPERKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGTALGMPDEV
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
BASE COUNT         4989 a         2618 c         2843 g         4382 t
ORIGIN      
        1 gaatacaaat gaaattgaca aattactaat accttatctt gataagaaat atggagaggt
       61 ttcgagtgaa gtagcaaaat tattaagaca aatgagaaat aaaatcggac atggtgattt
      121 caaaggtttt aacgaaaaag cagaaaaatt cgctcaaaaa tttatgaaac actttcattt
      181 tgactataca gaatacagta gattaaattg ggtattactt catacctgct gtcttttaga
      241 tgatttactc agaataacta tttttcaaca attaaaagtc acaaaataat tattatttta
      301 tagagcaatc acctattttg attgctcttt ttctattttt acggaaagga cgtgaagaca
      361 tgaaaaaaat aagaagttac accagtattt ggtcagtaga aaaagtgatt tatgcaatta
      421 atgatttaca actccctttt cccataacct ttacacaaat gacatggttt atcgtttctt
      481 tgtttgtcgt tataatcttt ggcagtttac caccactttc cttgattgac ggagcactac
      541 tcaaatactt agggattccc gttgtcctca cttggttcat gtgtcaaaaa acctttgacg
      601 ggaagaaacc ctatggattt ttaaaatcta ttattaccta tgctctacgc cctaaaataa
      661 cctatgctgg caaacaaata cgattaaata aagaaaaaat gaatgaatca atcacgattg
      721 taaggagtga acaattaaat ggcacacccc attaaatata tcgaagataa tctcgtattt
      781 aatcatgatg gcgaagtatt tgcttattat gaattaattc cctataatta cagtttttta
      841 agttccgatg aaaaagttca agttcatgat aatttccgtc aattgattgc ccaaaatcgt
      901 gatggtaaga ttcatgccct gcaactagca accgaatcaa gtattcgagc gacacaggaa
      961 caatcgaaaa aagaaatcac tggacgatta aaagagatag cttacgaaaa aatagatgaa
     1021 cagaccgaag cgttagtttc tatgattggt gacaatcaag tggactatcg cttttttatt
     1081 ggttttaaac tcttgctgaa tgaacaggaa gtatcggtca aaaatattgg caaagacatt
     1141 caaaaagcat ttgcggaatt ctttcatggc gtgaatcaca agttgatggg cgattttgtt
     1201 tctatgccta tgtcagaaat cgaacgcttt tctaaaatgg aacagttatt agaaagtaaa
     1261 ttatccagac gctttcacat tcgcccattg gggaaagatg attttgggta tatcattgcc
     1321 cacctttacg gtcaaacagg gattcctttt gatgactacg actaccattt acccaaacga
     1381 tatgaagata aaaaagtgtt ggtaaagaaa tacgatatta ttaaacccac tcgctgttta
     1441 atcgaagaaa agcaacgcta cttacggatg gaacaggaag atactaccac ttatgtggcg
     1501 tattttacga ttaacagtat cgtcggcgaa ttggatttcc ccaatagtga gattttttat
     1561 taccagcaac aacagttcac tttcccgatt gataccagca tgaatgttga aattgttgct
     1621 aataaaaaag ctctgtctac cgttcgcaac aaaaagaaag aattgaaaga cttggatagt
     1681 cacgcatggg aaagcgacaa tgaaaccagt accaatgtgg tggacgcttt agaatctgtc
     1741 gacgaattag aaaccaacct agaccaaacc aaagaatcca tgtacaaact aagctatgtg
     1801 gtacgggtat cggcaaaaaa tctggacgag ttgaaacaac gttgtgatga agtaaaagat
     1861 ttttatgatg atttgtctat caaattagta cgaccttttg gcgatatgct aggcttgcat
     1921 aatgaattta ttccctcaag caagcgatat atcaatgact atattcaata tgtcacgtcg
     1981 gattttttgg ctggtcttgg tttcggagct acacaaatgc ttggtgaaaa ttctggtatt
     2041 tacgtcggtt acaatctcga tacaggcaga aatatttatc tccaacccag ccttgcaagt
     2101 caaggaatta aaggctctgt gacgaatgcg ttagcgtctg cttttattgg ttcattaggt
     2161 ggcggaaaat cattctctaa caatatgctg gtctattatt cggtactctt tggcggacag
     2221 gcaatcattg tagacccaaa agctgaacgt ggcaaatgga aagaaacctt acctgatatt
     2281 gcccatgaaa tcaatatcgt taatctcacg agtgagaacg acaataaagg gttacttgac
     2341 ccttatgtga tcttaaaact cccgaaagat tcagaaagcc tagccattga tattctcacg
     2401 ttcttaacag gcatttccag ccgagatagc gagaagtttc ctgtcctgcg taaagcgatt
     2461 cggagtgtca ctcaaagcga acaacgaggg ttgttattgg tgattgatga attgcgaaaa
     2521 gaagatacac ccatcagcaa tagtattgct gaccatatcg aaagctttgt cgattatgat
     2581 tttgcccatc tgttattcag tgatggcaat gtcacccaat ccattagctt ggaaaaacaa
     2641 ctcaatatta ttcaagtggc agatttggtc ttacccgatt ccaatagctc ttttgaagaa
     2701 tacaccacca tggaattatt aagtgtggca atgctgattg tcattagtac ctttgcttta
     2761 gactttatcc actctgaccg ttccattttt aaaattgttg accttgatga agcttggagt
     2821 tttttacaag tggcacaagg aaaagctcta tctatgcgtt tagttcgggc agggcgtgcc
     2881 atgaatgcag gggtctattt cgttactcaa aacgccgacg atttactaga tgaaaagatg
     2941 aagaataata ttggcttgaa attcgccttt cgttccactg acctagtgga aatcaaaaag
     3001 accttagaat tttttggcgt ggacaaggaa gatgaaaaca atcagaaacg cttacgtgaa
     3061 ttagaaaatg gacaatgttt agtttcagac atctatggtc gtgtaggggt tatgcagttt
     3121 cacccgattt ttgaagagtt gcttcatgcc tttgatacca gaccgcctgt gcgaaatgag
     3181 ggggaaaaat gacacaaaaa caaaagaaat ttcttcacta tagttggata acgctccttt
     3241 gtgtgggcgt tattttactt ttattaggca cgctgggcaa tgcagtacaa gcaacaggct
     3301 tagttgatga aaccattgat acaagcaacg agtattccaa gtatggttta aatcattatc
     3361 agcttgatta ttatgtggac aatagctggg gctggctgcc gtggaattgg agtgacggca
     3421 tagggcaatc agtgatgtat ggactgtatg ccattaccaa tttcatttgg acgatttctc
     3481 tttatctttc caacgccaca ggctatttgg tcaaagaagc ctacgccttg gatttcatta
     3541 gtcaaacgtc tcaagcgatt ggtgagaaca tgcagaccat tgcagggatt acaccgaatg
     3601 gcttttccag ttcgggtttt tatgtcggct tccttcttct ttttattctg attgttggaa
     3661 tctacgttgc ctatacagga ctggtgaaac gagaaacgac caaagctata cgagctgtgt
     3721 taaattttgt ggtggtgttt attctatcag cttcctttat tgcgtatgct cccgattata
     3781 ttgcgaaaat caatgatttt tccaaagatg tgagcactgc cagtttagat attgggacaa
     3841 agattgtcct tcccgatacg gatagtcaag gcaaagacag tgtggatatg atacgtgata
     3901 gcctattttc tgttcaagtc aagcaaccgt ggttattact tcaatatggc tcaaccgata
     3961 tagaaacctt aggtgaagac cgtgtggaaa agttattggc taccagtcca gatacaaaca
     4021 atggtgaaga tagagaaaat gtagtgattg aagaaattga agatcatgac aatacctatt
     4081 taacactccc gaagaccatt tcccgattag gaacggtctt ctttttgttt gtattcaata
     4141 ttggtatctc gatttttgtt ttccttctaa caggaatcat gattttttcg caagtgctat
     4201 ttattatttt cgctatgttt ttgccaatca gtttcctatt aagtatgttg ccgagttttg
     4261 atggcatggg caggcatgcc attaccaagt tatttaatgt gattatgaca agggctggta
     4321 ttaccttgat tattaccgtt gcctttagta tttccactat gctttattcg ctgtcagcaa
     4381 gttctccctt tttcatgatt atgtttttac aaattgtgac ctttgcaggg atttatttta
     4441 aactgggcga tttaatgagc ctattctctt tacaaagcaa cgactctcaa aacatgggca
     4501 gacaagtgtt tcgcagacct cgtcaaatga tgaatcgaca aaccagaaga tttcaacgca
     4561 gtatgagtaa gatttttcgt ggaaaagcag ggtcaacgaa gcaaaagaca atgacaacaa
     4621 agcagaatca aaccaataaa gctaaccata ctcgtcaaaa tgaacggact acaccaaaga
     4681 aagaaacagc actttccaat gtgaaaaagc aacaaggcgg tatcgggcaa cgattaggtg
     4741 aaaagacagc ccatgtattg gacacgaaaa atcgtctagt caataaaaga aagcaggcta
     4801 aacaacagat acaggcgaca ccaacccatg tcaaatatgc ccttcatcaa ggaaaagaga
     4861 atgtcactca taatgtaaca gactttaaac aatctatggc agataccaaa gctcaacgca
     4921 aacaacagcg tatgaaccag caggagcaaa aacgaaagaa cattgctcaa aagcgtttgg
     4981 agctggataa agcaaaagaa aaaagacgaa aagaaaaccc atcatctcct gttcagaata
     5041 accagcagca tgttcgcaag cgtcagcaaa ccgtcacgcc tgttttgtca caaaacacta
     5101 aacgtgaaaa tcatcagcga ccattgaaaa aagagcaaaa acaaaagaca ccaccgaagc
     5161 aattcaatca gaggaagcta aataaaagaa cggataagcg aggacaacaa acatgaagct
     5221 aaaaataaca gtgatttgta gtgtggtctt ctctctcttc ttttttctct tattattcct
     5281 agtcattttc tttgcagatg atacggacag tggcgaaaac aacaaggatt cttctatttc
     5341 acaaggcggt gtgaccgttt caccagaagt gttggcacac cgtccgttga ttgaaaaata
     5401 tggtaaggaa tatgggattg aagactatgt ttcttatatc ttagccatta tgcaagtgga
     5461 atcgggcggt acagcagaag atgttatgca aagttccgaa tctttgggtt taccgcccaa
     5521 cagtttgagt accgaagagt ccatcaaaca aggggttaaa tatttcagtg aattattaac
     5581 cagtgcagaa caacaaggag tggatataga ttctgttatc caatcttata attatggcgg
     5641 tggcttttta aattatgtga ggagtcatgg gaaaaaatat acctatgaat tagccgaaca
     5701 gttttctaaa gagaagtcgg gtggtcaaaa agcagactat cccaatccca tagccatacc
     5761 tgtgaatggt ggttggcgat ataactatgg aaatcagttt tatgttcagt tggtttcaca
     5821 atacctaacc gacacttccc caacagaatt tgatgatgaa accgtgcaag tcattatgga
     5881 cgaagccttg aaatacgaag ggtttcccta tgtctttgga ggggcgtcgc ctaccacctc
     5941 ttttgattgt agtggcttaa tacaatgggt gtatgacaaa gctggcattt ctctccctcg
     6001 tgtcgcccaa gatcagtatg acgctacgca agaaatctct atggaagaag cccaagcagg
     6061 cgacccaatc ggttatggcg acttaagcag tcgttattgg caagaccatc tgattggggc
     6121 tagacgtgtt attcataact aagaaaggaa tgaatcaaat gattataaaa atcgagcgaa
     6181 aagaaaagaa ggagaaacca aagaaatcta aaaaaccgag caagcaaaga aaagttccga
     6241 tgattaaagt tggcacgcat aagaaattaa cctttgtttt atgggtttta ttgattggta
     6301 gtgtcggatt tggaatctat aaaaatttta ctgccattga cactcatact gtccgtgaaa
     6361 cagaaatcat taaacagcag attgtcgata ccaaccaagt agaaagtttt gtgaaatcct
     6421 ttgcgaaaga ctacttttcg tggcaacaat cccaagaagc cattgataaa cgaaatgaaa
     6481 aattgaccca ttatttgacg gaagaattgc aggtattaaa tgaagaaatg attcgtaagg
     6541 atattcctac tagttctagt gtgaacgata tacaggtgtg gcaggtttct caagtgaatg
     6601 agaacacctt tgaggtactg ttttctgttg aacaagttat taccgaagac aaagacaagg
     6661 aaactatatc gtctagcttt catgtggttg tccatataga tgaatcagat aatatggtta
     6721 ttatcaaaaa tccgacaatg agtaagaaac ctcaaaaatc ggactaccaa cccaaacagc
     6781 tagaaagtga ccatacagta gatacagaaa cgatggacga aatcactagc tttttagaaa
     6841 cgttcttcca gctctatcct accgccacag aaaaagaatt gacttactat gtaagtaatc
     6901 atgtgttacc tatgattaac aaggaatatg ttttcgagga attggtgaat cctatcttta
     6961 ccagaaaaga taaccaagta atcgtgaatg tcgctgtgaa atatttagat caagaaacaa
     7021 aggcaaccca aatctcgcaa tttgagttga tattagaaaa acaagataat tggaagattg
     7081 taaaataaca aatattggta catgattaca gatactttgt aatcatgtac tctttttgat
     7141 aaaaaattgg agattccttt acaaatatgc tcttacgtgc tattatttaa gtgactattt
     7201 aaaaggagtt aataaatatg cggcaaggta ttcttaaata aactgtcaat ttgatagcgg
     7261 gaacaaataa ttagatgtcc ttttttagga gggcttagtt ttttgtaccc agtttaagaa
     7321 tacctttatc atgtgattct aaagtatcca gagaatatct gtatgctttg tatacctatg
     7381 gttatgcata aaaatcccag tgataaaagt atttatcact gggattttta tgcccttttg
     7441 ggtttttgaa tggaggaaaa tcacatgaaa attattaata ttggagtttt agctcatgtt
     7501 gatgcaggaa aaactacctt aacagaaagc ttattatata acagtggagc gattacagaa
     7561 ttaggaagcg tggacaaagg tacaacgagg acggataata cgcttttaga acgtcagaga
     7621 ggaattacaa ttcagacagg aataacctct tttcagtggg aaaatacgaa ggtgaacatc
     7681 atagacacgc caggacatat ggatttctta gcagaagtat atcgttcatt atcagtttta
     7741 gatggggcaa ttctactgat ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta
     7801 tttcatgcac ttaggaaaat ggggattccc acaatctttt ttatcaataa gattgaccaa
     7861 aatggaattg atttatcaac ggtttatcag gatattaaag agaaactttc tgccgaaatt
     7921 gtaatcaaac agaaggtaga actgtatcct aatatgtgtg tgacgaactt taccgaatct
     7981 gaacaatggg atacggtaat agagggaaac gatgaccttt tagagaaata tatgtccggt
     8041 aaatcattag aagcattgga actcgaacaa gaggaaagca taagatttca gaattgttct
     8101 ctgttccctc tttatcatgg aagtgcaaaa agtaatatag ggattgataa ccttatagaa
     8161 gttattacta ataaatttta ttcatcaaca catcgaggtc cgtctgaact ttgcggaaat
     8221 gttttcaaaa ttgaatatac aaaaaaaaga caacgtcttg catatatacg cctttatagt
     8281 ggagtactac atttacgaga ttcggttaga gtatcagaaa aagaaaaaat aaaagttaca
     8341 gaaatgtata cttcaataaa tggtgaatta tgtaagattg atagagctta ttctggagaa
     8401 attgttattt tgcaaaatga gtttttgaag ttaaatagtg ttcttggaga tacaaaacta
     8461 ttgccacaga gaaaaaagat tgaaaatccg caccctctac tacaaacaac tgttgaaccg
     8521 agtaaacctg aacagagaga aatgttgctt gatgcccttt tggaaatctc agatagtgat
     8581 ccgcttctac gatattacgt ggattctacg acacatgaaa ttatactttc tttcttaggg
     8641 aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa
     8701 ctaaaagagc ctacagtcat ttatatggag agaccgttaa aaaatgcaga atataccatt
     8761 cacatcgaag tgccgccaaa tcctttctgg gcttccattg gtttatctgt atcaccgctt
     8821 ccgttgggaa gtggaatgca gtatgagagc tcggtttctc ttggatactt aaatcaatca
     8881 tttcaaaatg cagttatgga agggatacgc tatggttgcg aacaaggatt atatggttgg
     8941 aatgtgacgg attgtaaaat ctgttttaag tacggtttat actatagccc tgttagtact
     9001 ccagcagatt ttcggatgct tactcctatt gtactggagc aagcctttag aaaagctgga
     9061 acagaattgt tagagccata tcttagtttt aaagtttatg caccacagga atatctttca
     9121 cgggcatata acgatgctcc caaatattgt gcaaatatcg taaatactca actgaaaaat
     9181 aatgaggtca ttattattgg agaaattcct gctcgatgta ttcaagatta tcgcaatgat
     9241 ttaacttttt ttacaaatgg gcttagtgtt tgtttagcag agctaaaagg atatcaggtt
     9301 accactggcg aacctgtttg ccagacccgt cgtctaaata gtcggataga taaagtaaga
     9361 tatatgttca ataaaataac ttagtgcgtt ttatgttgtt atataaatat ggtttcttat
     9421 taaataagat gaaatattct ttaatataga tttgaattaa agtggaaagg aggagattgt
     9481 tattataaac tacaagtgga tattgtgtcc tatttgtgga aataaaacaa gactacgaat
     9541 acgagtggat actatactta aaaatttccc tttatactgc cccaaatgta agaacgaaac
     9601 tttaattaat gttcaaaaaa tgaatataat aacaatcaaa gagccagacg ccaagacgca
     9661 gagccgataa tttgagaaat gaaactctca tcttatcggc tctttttgtt tatctgaatt
     9721 ttactgacta gccttcaata tttcttgctt cattgatacc ttttgctagt gcttccatta
     9781 aggatagttc tttgtctgta aagctatcca tgtatttttc tatctgtagc cttcgagtgc
     9841 tttttatcaa attatctgta ggtaagaaaa attcatcaat agacacatgg agtaatgata
     9901 caagatcata aagcacctgt gtacttgggt gttgcccttt attttctata tttgttaaat
     9961 aacgtgggtc aatttcaatg attgttccta cttgttcacg ggtgagacca cgttttattc
    10021 gagcctcttt aatcgctaga ccaacggctc taaaatcgta tttatcttct tttttacgca
    10081 taataaacca cctctataca ttttactgtt cctatccaag tggaaacagg tatagaaaaa
    10141 cgtgttatgt agtttgtgag ttcttttaag agcgctatta tgcttatgaa tgtgataaat
    10201 ccaatctcaa atagaaaggt cgctgatttt atttacccat acaactatat aaaatttagt
    10261 tatgctgcta aaaagtagat aaaattggcg actgaatgga ggtcacagtc gccatgaaaa
    10321 agatacgtca ctatctaatt ctcttaatta ggttcttatc aaaaaaactt acccacctac
    10381 actagataaa tatgaccatc aatgtggttt tacaaatcgt atagacagaa caattgaagc
    10441 acatgaacag agaaaacaat ctgtttatgt gtttttttgg ctacttcgtg aatatctttt
    10501 aagatttttc ttcacttatg caacaaatcc ccctttcgtg tcgggactta gtatgaaggg
    10561 agataaagaa gtacatgcca ctccctttca cgacagaaag ggggtgaaaa acatgaaacc
    10621 atcttctttt caagaaacca tagaaaatca gtttgactat atctgcaaac aagccattga
    10681 agatgaacgg aaagattatt tcaaacatct aagcagactg tccaaaaaag aagtttcatt
    10741 ttctgaaata gataactacc ttgtcacctc tttttctacg gttgatacgt atgtcactga
    10801 ttttcaacta ttcacattgt atggattaag aatcaatgtt gaaagtgatt tgttaagcga
    10861 agccttacgc caccttccag agaacaagcg gaacatcatt ttacttcatt attttatgga
    10921 tatgagtgat gtggaaattg cagaattact aaatgtaaat cgttcaacag tttatcgtca
    10981 tcgaataagt ggactagcca tgattaaaga attcatgaag gagtgtaaag aatgaaaaca
    11041 gcctatccaa gggttccctt ccccctcatt gtaaaggcga cagatggcga cgtggaagca
    11101 attaatcaga ttgttaaaca ttatcgaggg tatacctcaa aacgttcttt gcgtcgcatg
    11161 acagacgaat atggcaattc tcatatggtt attgatgaaa ccctacgtgg cagaatggaa
    11221 acaaaactca tcaccaagat tttagcgttc gaaattaaat aatctcattt tttcgtggaa
    11281 gcgtgttgaa ccgttccacg cttcccgatt tgggaggttt gttattccat caaagcctac
    11341 tgtatgttca gtatgttttg ataggctaac aagccaaacg ttctttgaca attgaataaa
    11401 gccaatcgaa tacgtttcga taagaaaaga gccaacgaac taacacgcca tgatctatga
    11461 agatagcgag cgattcatgt taaaacttcg taaacaatct ttagcaggat tgttgccatg
    11521 actttcttat cgtgataatg atactcccgt atagccacag tccgagcgtt agtagcgtcg
    11581 cagacaatgg gtacggctac atgaaaatca tgcaggggtg aaatgcccgt gagctttgct
    11641 agagctgttc gattgctggg atattcttct cttgtcaatg tattatcata aaaatatttg
    11701 atataattat aggtgaatat tggagttttc ctagcctata atgggaaagg agtaataatg
    11761 ataacggtca caaaaaaaga tttagttgag ctaggatatg gaacttcttt ttcagcagac
    11821 atcataaaaa aagcaaaaga attgatgatt acaaaagggc atacctacta tcaatctcga
    11881 aagttagata gagtccctcg tgaagctgtt gaagaaattc taggaattcg gttttcaaat
    11941 gaaaacaggt ctgagtagtt tttgcacagt aaggagtaaa aactatggtt aaagacccaa
    12001 taaaaaaagc aaagaatgga acctattatt ttcgtgcaaa tctaggttat gattctaaag
    12061 gaaaaaaaat tcaaaaatat cgtagtggat tcactactaa aaaagaagca agagaagaat
    12121 attcaaaact actactgatg aaaccagaag aattaagtga gaataaggat aaaatgacgt
    12181 ttaatcatta tatctttgaa atttttcttc catggtataa gacacaggta aagctcagaa
    12241 catatgaaaa tcgcttacca actattaaga aacatttctc ttattttgat aaaatggctg
    12301 tttccgatat tgagcctatt gatgtgcaaa attggcaact aaaattatca aagaaatgta
    12361 agtcttctta tgtgagagct gttcaaggac tattttcagt tgctatggat cgagcaattg
    12421 tccttgggat aaccaccaca aatccatcta agattattgg caatgtgaaa aagcaaaagt
    12481 ctaaaattga attttggaca aaagaagaat ttgaaaaagt aatctctcta atttataaag
    12541 aagattacta tcaacacttt ttatttatct ctctgtggtt tttatttatg actggaatgc
    12601 gaattggtga agctactgcc attcaatggg aagatattga ttttgactct ggagtactaa
    12661 ccatcaacaa aacactttat tacaagaatc aatcggatta tagatttgtt gaaccaaaaa
    12721 caaaggcgag tgttcgtcat attgtattag atgaatgtac tctaaattat ctatccgaat
    12781 ggaagaaagc acaacaaaat ctaattcaga cagactttgt aatgagctat aacggtattc
    12841 ctacccaaaa acatacgcta gcacatgcta ttgaacgcta tgctaagctg gctagtattc
    12901 atcgaatcag aattcatggt ttacggcatt cgcatgcttc cttgttaatc agtatgggag
    12961 aaaacccact catcattaaa gaccgtttag gacatgaaga tattgagacg actttaggaa
    13021 cgtatggaca tctttatcct aacagtaatt ttgaggtagc taacaaatta aatggagcca
    13081 tttcctttaa agaagcaaaa caaaattatg atacttcacc aaagaatcaa tttactgtag
    13141 actacttgcg aaacaaagaa atgaaaaaag tgcaataaaa gtgcagtaaa aaaataagaa
    13201 gagccagaaa cccttgataa acaaggattt ctggctctcc tacaactact cccactcaac
    13261 agttgcaggt ggtttacttg tgatatcata aacaatacgg ttaacgtgat caacttcgtt
    13321 aacaatacgt gtcgaaattt tcttaagtac atcccaagga agttgcgcaa aatcagctgt
    13381 catgccatca attgaagtaa ttgcacggat agcaattgta tagtcataag tacgaccatc
    13441 tcccataaca ccgacagaac gtacgccagt attaactgtg aaatactgcc atacgtcacg
    13501 atctagacca gctttagcga tttcttcacg aagaatagca tctgattcgc ggactgtttc
    13561 aagtttttct tcggtaattt cacccataac gcgaatagca agaccaggtc cagggaatgg
    13621 ttgacgccat acaacttcat caggcattcc aagtgcagta ccaagagcgc gaacctcatc
    13681 tttgaataat gtgttaagtg gctcaatcaa ttcaaattgc atatcttctg gcaatccacc
    13741 aacattatgg tgtgacttga ttgtttgggc tgtttctgtc ccagattcaa taatatctgt
    13801 gtagagggta ccttgagcaa ggaaatcaac gcctttaagt ttacttgcct catcatcaaa
    13861 aacgtacacg aactcatttc cgatgatttt tcgttttctc tcgggatctt caacaccaga
    13921 aagaaggtct aggaagcgtt ttgaagcatc aacacggata atattaagtc caaatttacc
    13981 accaagcata tccataactt ggtcaccttc atttttacgt aataaaccat gatctacaaa
    14041 aatacatgtt aattgatcac cgatagcacg ttgtaggagt actccgacaa cagatgaatc
    14101 aacaccgcca gatagtccaa gaagtacttt acgatcgcct acagtttcac gtatttttgc
    14161 aatttccata tcaatgaagt tatccattga ccaatcacca cgtgcgccac agatattaac
    14221 agcaaaattt ttaaggatat cattgccata tactgagtga cgaacttcgg ggtggaattg
    14281 gataccataa aattgttttt cagtattttc cattgcagca aatggacagt caacagagtc
    14341 accaacgaga tggaaaccct caggaatttc agtgacagca tcaccatggc tcatcaaaac
    14401 aagttgttct tgaggagtgc cagcaaagag tgctgattct gagcgtaggc gaagtgctga
    14461 ttgaccatac tcacgatgac cagcttctcc cgcaggaaga accttaccac cgagtttatg
    14521 ggtaatcagt tgcataccat aacaaattcc taagattgga attcctaatt caaaaatttc
    14581 ttcatcgatt ccaaaagcgc catcagcgta tacggaattt ggaccacctg aaagtacgat
    14641 accaatagga ttaatatcgc gaatttcatc agccgtaatt ttatgtgatt ttaactcaga
    14701 aaatacacca aattcacgga tacgacgtgc gataagctgg ttatattgac taccgtaatc
    14761 tagaacgata attttttgga tatcattcaa tatagaaata tcagtcattg tttccctttc
    14821 tttgtagcta at
//
LOCUS       DATRTN010000040        14448 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.040, whole genome shotgun sequence.
ACCESSION   DATRTN010000040 DATRTN010000000
VERSION     DATRTN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14448
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.040"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            215..1060
                     /locus_tag="VBE22_001203"
     CDS             215..1060
                     /locus_tag="VBE22_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HEO7202669.1"
                     /translation="MLSVGLVLEGGGMRGLYTAGVLDAFLDAGIKIDGIVSVSAGALF
                     GVNFVSRQRERALRYNKKYLSHPKYMSLRSWFRTGNFVNKDFTYYEVPMKLDVFDDEA
                     FKKSSIDFYVVATEMTSGKPEYFKIDSVFEQMEILRASSALPVVSKMVDWQGKKYLDG
                     GLSDSIPVDFARGLGFDKLIVVMTRPLNYQKKPSSGRLYKTLYRKYPNFVKTASNRYQ
                     QYNNSLEKVMSLEKTGDLFAIRPSKSLVIGRLEKNPDKLDSIYQLGMKDAKSVMPELN
                     SYLMK"
     gene            complement(1103..2350)
                     /locus_tag="VBE22_001204"
     CDS             complement(1103..2350)
                     /locus_tag="VBE22_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7202670.1"
                     /translation="MSPLNINKNNWRALIAAIVASGTDDLNIMFLAFSMSTIITDLHL
                     SAAQAGWIGTITNLGMLVGGLIFGLLADRYNKFKVFKWTILIFSIATGLVFFTTNLSY
                     LYIMRFIAGIGVGGEYGIAIAIMAGIVPTNKMGRISSLNGIAGQVGSISSALLAGWLA
                     PALGWRGLFLFGLLPIVLVLWMQFAVDDKDILDQYNTDADDEPLDISIKALFDTPVLA
                     TQSLALMVMTTVQIAGYFGMMNWLPTIIQTNLNVSVKNSSLWMIATILGMCLGMLVFG
                     QLLDKFGPRLVYGCFLLSSAICVYLFQFATTMPSMIIGGAVVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNLILNVGRAIGGFSSVIIGMILDVSNVSMVMLFLASLYIVSFLSML
                     SIKQLKRQKYHTNLTQLDVKPTD"
     gene            2727..5228
                     /locus_tag="VBE22_001205"
     CDS             2727..5228
                     /locus_tag="VBE22_001205"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEO7202671.1"
                     /translation="MTFYNHKEIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFNLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKAAA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGQSISYVPWPSYDESKLVENEIEIVVQIKGKVKAKLVVAKDLSREEL
                     QDLALANEKVQAEIAGKDIIKVIAVPNKLVNIVVK"
     gene            complement(5315..5923)
                     /locus_tag="VBE22_001206"
     CDS             complement(5315..5923)
                     /locus_tag="VBE22_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome assembly protein"
                     /protein_id="HEO7202672.1"
                     /translation="MLHSKKIHSLSLIAVLSLATYTSLQPNHVTAEQSQKTSTVHMSQ
                     KTIEHKLKVADKEAAPLYAKIDHIQRHIEVKKAKDLKVIELYINKDINQLEKQNKRLL
                     TKFYTSIDNQTWDSTSEVKKLIDKTNLSTNEKDRLKLYFEQRAYLETRLNDRYQKFDN
                     SIENQNKELKILTSKIEKIYQKHGITKEVLKTYYAKKTVRAD"
     gene            complement(6016..7404)
                     /locus_tag="VBE22_001207"
     CDS             complement(6016..7404)
                     /locus_tag="VBE22_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7202673.1"
                     /translation="MKKKSLISNLILINSITLFLGFYLIYFSVNYVIKDYVHQMTRNS
                     MQANFTILDSIYNNKKIPDENMVNQDSIFVWSYYAIFDQHYRMKYGTDNHKKESHDII
                     RYLTQKRLWQISKEKDGMFVTIKKKTYYVMTKDYSGILVDGSIKKVPKAQSQLFHVIN
                     FSDITYTQHLITKINHFLIVILVLTYIPMLFIMRKTFTGIRESIQSVQTYISSLWKNQ
                     GNHQSSQKEIVFSDFDPLLLESQEMANRIYQAEESQRNFFQNASHELRTPLMSIQGYT
                     EGVQEGIIDAELAHSVILQESKKMKQLVDDIILLSKLDSNLSDQKDEFSLNELLNSII
                     AYFKPLANKQKISITYRPDKHEKLLKGNEELIQRAINNILSNALRYAVSHIEISYTNQ
                     KLTISNDGPAISKEDLPYIFDRFYKGHGGQTGIGLAMTKEIIKQHHGNIIAESDSTST
                     TFTIYFNCHKIA"
     gene            complement(7401..8087)
                     /locus_tag="VBE22_001208"
     CDS             complement(7401..8087)
                     /locus_tag="VBE22_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7202674.1"
                     /translation="MTKIIYFADDEKNIRDLVVPFLEHDGFTVRAFETGDLLLEAYKN
                     QKPDLVILDIMMPGTNGLDVMKSIRQYDNIPIIMLTARDSDVDFITAFNLGTDDYFTK
                     PFSPIKLSLHVKALFKRLDEKAIKNDTQYQFLDLTLDTEKRIALLSNEEMPLTKTEFD
                     FLLVLIEKPETAFSRETLLNRIWGFDDIESRAVDDTIKRLRKKFKQYHSQVSIKTVWG
                     YGFKLIKEDK"
     gene            complement(8320..13032)
                     /locus_tag="VBE22_001209"
     CDS             complement(8320..13032)
                     /locus_tag="VBE22_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO7202675.1"
                     /translation="MNTKQRFSIRKYKLGAVSVLLGTLFFLGGITNVAADSVINKPSD
                     IAVEQQVKDSPTSIANETPTNNTSSALASTAQDNLVTKANNSPTETQPVAESHSQATE
                     TFSPVANQPVESTQEVSKTPLTKQNLAVKSTPAISKETPQNIDSNKIITVPKVWNTGY
                     KGEGTVVAIIDSGLDINHDALQLNDSTKAKYQNEQQMNAAKAKAGINYGKWYNNKVIF
                     GHNYVDVNTELKEVKSTSHGMHVTSIATANPSKKDTNELIYGVAPEAQVMFMRVFSDE
                     KRGTGPALYVKAIEDAVKLGADSINLSLGGANGSLVNADDRLIKALEMARLAGVSVVI
                     AAGNDGTFGSGASKPSALYPDYGLVGSPSTAREAISVASYNNTTLVNKVFNIIGLENN
                     RNLNNGLAAYADPKVSDKTFEVGKQYDYVFVGKGNDNDYKDKTLNGKIALIERGDITF
                     TKKVVNAINHGAVGAIIFNNKAGEANLTMSLDPEASAIPAIFTQKEFGDVLAKNNYKI
                     VFNNIKNKQANPNAGVLSDFSSWGLTADGQLKPDLSAPGGSIYAAINDNEYDMMSGTS
                     MASPHVAGATALVKQYLLKEHPELKKGDIERTVKYLLMSTAKAHLNKDTGAYTSPRQQ
                     GAGIIDVAAAVQTGLYLTGGENNYGSVTLGNIKDKISFDVTVHNINKVAKDLHYTTYL
                     NTDQVKDGFVTLAPQQLGTFTGKTIRIEPGQTQTITIDIDVSKYHDMLKKVMPNGYFL
                     EGYVRFTDPVDGGEVLSIPYVGFKGEFQNLEVLEKSIYKLVANKEKGFYFQPKQTNEV
                     PGSEDYTALMTTSSEPIYSTDGTSPIQLKALGSYKSIDGKWILQLDQKGQPHLAISPN
                     DDQNQDAVAVKGVFLRNFNNLRAKVYRADDVNLQKPLWVSAPQAGDKNYYSGNTENPK
                     STFLYDTEWKGTTTDGIPLEDGKYKYVLTYYSDVPGSKPQQMVFDITLDRQDPTLTTA
                     TYDKDRRIFKARPAVEHGESGIFREQVFYLKKDKDGHYNSVLRQQGEDGILVEDNKVF
                     IKQEKDGSFILPKEVNDFSHVYYTVEDYAGNLVSAKLEDLINIGNKNGLVNVKVFSPE
                     LNSNVDIDFSYSVKDDKGNIIKKQHHGKDLNLLKLPFGTYTFDLFLYDEERANLISPK
                     SVTVTISEKDSLKDVLFKVNLLKKAALLVEFDKLLPKGATVQLVTKTNTVVDLPKATY
                     SPTDYGKNIPVGDYRLNVTLPSGYSTLENLDDLLVSVKEDQVNLTKLTLINKAPLINA
                     LAEQTDIITQPVFYNAGTHLKNNYLANLEKAQTLIKNRVEQTSIDNAIAALRESRQAL
                     NGKETDTSLLAKAILAETEIKGNYQFVNASPLSQSTYINQVQLAKNLLQKPNVTQSEV
                     DKALENLDIAKNQLNGHETDYSGLHHMIIKANVLKQTSSKYQNASQFAKENYNNLIKK
                     AELLLSNRQATQAQVEELLNQIKATEQELDGRDRVSSAENYSQSLNDNDSLNTTPINP
                     PNQPQALIFKKGMTKESEVAQKRVLGVTSQTDNQKVKTNKLPKTGESTPKITYTILLF
                     SLSMLGLATIKLKSIKRE"
     gene            13355..14047
                     /locus_tag="VBE22_001210"
     CDS             13355..14047
                     /locus_tag="VBE22_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEO7202676.1"
                     /translation="MEKGFKEGVKDALPTALGYISIGLAFGIVASASDLSAIEVGLMS
                     ALVYGGSAQFAMCALLLAKADLMTITMTVFLVNLRNMLMSLHATTIFKSAHLMNQLAI
                     GTLITDESYGVLLGEALHHKVVSPSWMHGNNVMSYLTWVISTIIGTLLGSTIPNPEMF
                     GLDFALVAMFIGLFVFQLFGMLSDGKRLVVYVLASVGLSYFLLATFLSGALSVLLATV
                     VGCSVGVVLDDK"
     gene            14037..14360
                     /locus_tag="VBE22_001211"
     CDS             14037..14360
                     /locus_tag="VBE22_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEO7202677.1"
                     /translation="MINSTVFLAILLGFVVSWVPRVAPFIFVKYKGLPNSLVKFLKFL
                     PLSILFALTFSSFFLVKLGQLPTLKWLDLLAGIPTLVVTIRYRNLFYTVLTGIVTMAL
                     LRLLF"
BASE COUNT         4288 a         2507 c         2785 g         4868 t
ORIGIN      
        1 gataagtcat aaaaaatcct ttatttatat tagttagata tagtaaatat aaatttaaaa
       61 actattagta ttattctatc ataggtgagt gtgattcaga aattattatt aaattaatta
      121 tggagtttat atagtacaac atattctttt tcagaaaaaa cagctatatt ttgctatact
      181 ttaatcaaat atcaatatta gaaagtgatt gcctatgttg tctgttggtt tagttttaga
      241 gggtggcgga atgagaggtc tttatactgc tggagtttta gatgcttttc tagatgcagg
      301 aataaaaata gatggtatcg tatctgtctc tgctggtgca ttgtttggtg ttaattttgt
      361 atctagacaa cgagagaggg ctttgcgata caataaaaag tatttatccc accctaaata
      421 tatgagtcta aggtcatggt ttcgaacagg gaattttgtt aataaagatt tcacctatta
      481 tgaagttcct atgaaattgg atgtatttga cgatgaagca tttaaaaaat caagtattga
      541 tttttacgta gttgctacag agatgacatc tggtaaacct gaatatttta aaattgatag
      601 tgtttttgaa caaatggaaa ttttacgtgc tagttcagca ttaccagtag tctcaaagat
      661 ggttgattgg caggggaaaa agtacttaga tggtggttta tctgatagta ttcccgttga
      721 ttttgcccgt ggtttaggat ttgacaagtt gattgttgtg atgactaggc cgctcaatta
      781 tcagaaaaag ccttcaagtg gacgattgta taaaactctg tataggaaat atcctaattt
      841 tgtaaagaca gcctcgaatc ggtaccaaca gtataataat agtcttgaaa aggtcatgag
      901 ccttgaaaaa acaggcgatc tatttgcaat tagaccgagt aagagcttgg ttattggccg
      961 cttagagaag aatccggata aacttgatag tatttatcag cttggtatga aagatgctaa
     1021 aagtgtgatg cctgagctga atagttatct aatgaaataa agaaaacagt atttctcaat
     1081 tgaggaatac tgttttgtaa tatcagtcag ttggtttgac atctaattgt gttaaattag
     1141 tgtgatattt ttgacgtttt aattgcttaa tgcttagcat tgataaaaaa ctaacgatat
     1201 agagacttgc taagaaaagc atgaccatag agacatttga aacgtctaga atcattccga
     1261 tgataacaga tgaaaagcca cctattgcac gaccaacatt taagataaga ttgttagctg
     1321 ttgaacgaat atgatgtgga tagagacgtg taatcatggc accatagcct gcaaacatac
     1381 cattaacaaa gaatccgaca actgccccgc ctataatcat agaaggcatt gttgttgcga
     1441 attgaaagag atagacacaa attgcagatg atagaaggaa acacccgtat actaaacgcg
     1501 gtccaaattt atctaacaat tgaccaaata ctagcatgcc aaggcacatt ccaagaatgg
     1561 ttgcaatcat ccataatgat gaattcttga cagaaacgtt taagttggtt tggataatag
     1621 ttggtaacca gttcatcatg ccaaagtatc cggcaatctg aacagttgtc ataaccatta
     1681 gtgctaggct ctgtgtggct aatacaggag tatcaaataa agcttttata ctgatatcta
     1741 gaggttcatc atctgcgtct gtattatatt gatctaagat atctttatca tcaacagcaa
     1801 attgcatcca taggacgaga actataggga gaaggccaaa gagaaaaagt ccccgccaac
     1861 cgagtgctgg agctagccat cctgctaaga gtgctgagct aatagatcca acttgaccag
     1921 cgataccatt tagtgaagaa atccggccca ttttattagt tgggacaatt cctgccataa
     1981 tggcaatagc gataccatat tcgccaccaa cgccaattcc tgcaatgaag cgcatgatat
     2041 agagatatga taaattagta gtgaagaaaa ctaaaccagt tgcgattgaa aagattagaa
     2101 tcgtccattt gaaaacttta aatttattgt agcgatcagc taataaacca aagattagtc
     2161 ctcccacgag cattcccaag ttggtaattg tcccaatcca accagcctgt gccgcagata
     2221 aatgaagatc agtaatgata gttgacatag aaaatgcaag gaacatgata tttaaatcat
     2281 cggttccgga tgcgacaatg gcagcgatta gggcgcgcca attattctta ttgatattta
     2341 atggtgacat aagtctcctc ctttaatgat ggctcacaga ccaccgttat taaatactgt
     2401 caatagaaaa aattctaaaa cagagatgtc atagctgctt caactcatta taatgtaaaa
     2461 gaaaaaactc aagctgctac ccttgagttc tataaacgct cgcaaaaata gtaggatttc
     2521 tgtatagaat cctattttgg tcgcttatat gtctcacagg acacggttaa agcattatgt
     2581 catttggaaa tagtatagag taagtttagt ttattgtcaa ggataagtgg ggagagaacc
     2641 tgctgttgag ggtattttca ccttttttat tgctattttt cgtgttataa taagaagact
     2701 atactttttt gacatggaga agtgcgatga cattctataa tcacaaagaa atcgaaccca
     2761 aatggcaagc tttttgggca gataatcata cctttaaaac aggaacagac gcatcaaaac
     2821 ctaagtttta tgccctagat atgttcccat acccatctgg agctggtctc cacgttggtc
     2881 acccagaagg ctatacagca acggatattc tcagccgttt caaacgtgcc caaggtcaca
     2941 atgtcctcca cccaatgggt tgggatgctt ttggacttcc tgctgagcag tacgctatgg
     3001 atactggtaa tgacccagct gagtttacag ctgaaaacat cgctaacttc aaacgccaga
     3061 ttaatgctct tggtttctca tacgactggg atcgtgaagt taacacaact gaccctaact
     3121 actacaagtg gacacagtgg atctttacca agctttatga aaaaggtcta gcttacgaag
     3181 ctgaagttcc agttaactgg gttgaagaat tgggaacagc tatcgccaac gaagaggttc
     3241 ttccagatgg tacatcagaa cgtggtggct acccagttgt cagaaaacca atgcgccaat
     3301 ggatgctcaa aatcactgcc tatgcagagc gcttgctgga agacttggaa gaagttgact
     3361 ggccagagtc aatcaaggac atgcaacgca actggattgg taaatcaact ggtgctaatg
     3421 taaccttcaa ggttaaggat acagataagg acttcactgt ctttacaaca cgtcctgaca
     3481 ccctttttgg tgcgacttac gcagttcttg ctcctgagca tgccttggtt gatgctatta
     3541 caacagctga ccaagcagaa gccgttgccg aatacaaacg tcaagcgagt ctcaaatcag
     3601 accttgctcg tacagacctt gccaaagaaa aaactggtgt ctggacaggt gcctatgcca
     3661 ttaacccagt taatggtaaa gaaattccag tttggattgc ggactatgtc cttgctagct
     3721 acggtacagg tgctatcatg gcagttccag ctcacgatga acgtgactgg gaattcgcaa
     3781 aacaattcaa cctagacatc attccagttc ttgaaggtgg gaatgttgaa gaggctgcct
     3841 ttacagaaga cggtcttcac atcaactcag acttcctaga tggtctcgac aaagcggcag
     3901 ctattgccaa aatggtcgag tggcttgaag cagaaggtgt tggtaatgag aaagttactt
     3961 accgtcttcg tgactggctc ttcagccgtc agcgttactg gggtgagcca atcccaatca
     4021 tccactggga agatggaaca tcaactgcgg ttcctgagag cgaattgcca ctagttcttc
     4081 cagttactaa ggatatccgt ccttcaggta ctggtgagag cccacttgct aacttgacag
     4141 actggttgga agtcactcgt gaagatggcg ttaaaggtcg tcgtgagacc aacaccatgc
     4201 cacaatgggc aggttcaagc tggtactatc tccgctacat cgatccacac aacactgaaa
     4261 aactagctga cgaggaactc ctcaaacaat ggttgccagt tgatatctat gtcggtggtg
     4321 cagagcatgc cgtgcttcac cttctctatg ctcgcttctg gcacaaggtt ctttatgatc
     4381 tcggtgtcgt acctaccaaa gagccattcc aaaaactctt taaccaaggg atgattttgg
     4441 gaacaagcta ccgcgacagc cgtggtgctc ttgtggcaac tgacaaggtt gaaaaacgtg
     4501 atggcagctt cttccatgtg gaaactggtg aagagcttga acaagcacct gctaagatgt
     4561 ctaaatcact taagaacgtt gtcaatccag atgatgttgt tgagcagtac ggtgctgaca
     4621 cccttcgtgt ttacgagatg ttcatgggac cactcgatgc ttcaatcgct tggtctgaag
     4681 aaggtcttga aggctcacgt aagttcttgg accgtgttta ccgtttgatc acgactaaag
     4741 agatcactga ggaaaatagc ggagctcttg acaaggttta caacgaaact gttaaggcag
     4801 ttactgagca agttgaccaa atgaaattca acacagctat cgctcaactc atggtctttg
     4861 tcaatgcagc caacaaggaa gacaaactct ttagcgacta tgccaaaggc tttgttcaat
     4921 tgattgcacc attcgcaccg cacttaggtg aagaactctg gcaagtcttg acagcctcag
     4981 gacagtcaat ctcttacgtg ccatggccaa gctatgacga aagcaaattg gttgaaaacg
     5041 aaatcgaaat cgttgtccaa atcaaaggta aggtcaaagc taaactcgtc gttgctaaag
     5101 acctttcacg cgaagaattg caagatcttg ctcttgctaa cgaaaaagtc caagccgaaa
     5161 tcgcaggcaa agacatcatc aaagtcatcg cagtaccaaa caaactcgtt aatattgtgg
     5221 tgaaataaga taaagatttt actagaaata gtgatattta cgcgtataaa atcccaaaag
     5281 tttaactttt gggatttttc tatttttagc taaatcagtc agctcgtact gtttttttag
     5341 catagtaagt ttttaatacc tcttttgtaa taccatgttt ttgatagatt ttttctattt
     5401 ttgacgttaa tatttttagt tctttatttt ggttttcaat agagttatca aatttttgat
     5461 agcggtcgtt caaccttgtc tcaaggtaag cacgttgttc aaaatataat tttaatctat
     5521 ctttttcgtt agtggatagg tttgtcttat caatcaattt tttgacttca cttgtgctat
     5581 cccatgtttg attatcaata gaagtataga atttagttag tagacgttta ttttgcttct
     5641 ctagttggtt gatatctttg ttaatataca attcaataac ttttaaatct tttgcttttt
     5701 tgacttcaat atgtcgttgg atatggtcga ttttagcgta gagaggagca gcttctttat
     5761 ctgcaacttt taacttatgt tcaatagttt tttgactcat atgaacagtt gatgtttttt
     5821 gtgattgttc agccgttaca tgatttggtt gtaaactcgt atatgttgct aaagagagaa
     5881 cggcaataag cgataaggaa tgtatttttt tagaatgtaa catataaaaa ctcactttct
     5941 tttagaataa ctttttgatc tataggtatt ataggttaaa aaagcaataa cttctttgaa
     6001 tttttgtagg aaaagttagg caattttgtg gcaattgaaa taaatcgtaa aagttgttga
     6061 ggtcgaatca ctctcagcga tgatattacc atgatgttgc ttgataattt ctttagtcat
     6121 tgcaagtcca atacctgttt ggccgccatg tcctttatag aagcgatcga aaatataagg
     6181 caagtcttct tttgaaatgg ctggtccgtc gtttgagata gttagttttt gatttgtgta
     6241 agagatttca atatgagaaa cagcatagcg tagtgcgtta ctaaggatat tattgatagc
     6301 tcgttgaatc aactcttcgt taccttttag taacttttca tgcttgtctg gtctataagt
     6361 aatactaatc ttttgtttat tagctagggg tttgaaataa gcaataatac tattaagtaa
     6421 ttcatttaaa gaaaattcat ctttttgatc acttaagtta ctatctaatt tagagagtaa
     6481 gataatatcg tctacgagct gtttcatttt tttgctctct tgcagaataa cagagtgtgc
     6541 gagctcggca tcaataatac cctcttgaac tccctcagta tatccttgga tggacattaa
     6601 aggtgtccgt aactcgtggg aggcattttg aaagaagttt ctttggctct cttctgcttg
     6661 gtagattcga tttgccattt cttggctctc aagtagaaga ggatcgaaat cagaaaagac
     6721 aatttctttt tgactagatt gatgattgcc ttgatttttc caaaggctgg aaatataagt
     6781 ttgtactgat tgaatagatt ctctaattcc agtgaaagtc ttcctcataa taaatagcat
     6841 gggaatatag gttagtacta agataactat aagaaaatga ttaattttag taattagatg
     6901 ctgggtgtaa gtaatgtctg aaaagttaat cacatgaaaa agttgcgact gtgctttagg
     6961 aacttttttt atgctaccat caacaaggat accagagtag tctttggtca ttacataata
     7021 ggtttttttc ttgatggtta caaacatacc atctttttcc ttagaaattt gccacaatct
     7081 tttttgggta agatagcgaa taatatcatg gctctctttt ttatgattat cagtgccata
     7141 tttcatacga taatgctggt caaaaatagc atagtaagac caaacaaaga tgctatcttg
     7201 gttaaccata ttttcatctg gtattttctt gttgttatag atactatcta aaatggtgaa
     7261 attcgcctgc atggaattcc ttgtcatttg atggacatag tctttaatga catagttgac
     7321 agaaaaatag atgaggtaaa aaccaagaaa tagagtgatg ctattaataa ggataagatt
     7381 gctaattaaa cttttctttt tcatttgtcc tctttgatta atttgaagcc gtaaccccag
     7441 acagttttga tactaacctg actgtggtat tgcttaaatt ttttacgtaa tcgtttaata
     7501 gtgtcatcaa ctgctctgct ttcaatatca tcaaatcccc aaatacgatt aagcagtgtt
     7561 tccctagaaa aagcagtttc aggtttttca attaatacta aaagaaaatc aaattctgtc
     7621 ttggttaagg gcatttcttc atttgataat agggctattc gcttttcggt atccagagtt
     7681 aaatctagga actgatactg agtgtcattt ttgattgctt tttcatcaag gcgtttgaac
     7741 aatgctttaa catgtagact aagttttata ggtgaaaaag gcttggtgaa gtaatcgtca
     7801 gttcctaaat taaaagccgt aatgaaatca acatcagaat ctctagctgt taacatgata
     7861 ataggaatat tatcatattg gcggatgctt ttcataacat ctaaaccatt agttccaggc
     7921 atcattatgt ctaatataac taaatcgggt ttttggtttt tataggcttc taagagaagg
     7981 tctccagttt caaatgctcg aacagtgaaa ccatcatgtt ctaaaaaagg aaccaccaag
     8041 tcacgaatat tcttttcatc atcagcgaaa taaattattt ttgtcatgta tacctcaata
     8101 cccatagtta taatttgact tttagctagc tgtttatgtc atatcaaata taacaaaaaa
     8161 agagtttttt tcccattctt tgaagtgtta tggtgttata aaccaagata ctgcagagaa
     8221 aaggtcttta ggaacatcat tttaagctcc tcaaaaaggc accagtcact aactattaat
     8281 agctagtaat tgatgccttg ctctattttt taaagtattt tattctcttt tgatagactt
     8341 tagtttgatt gttgccagac ctagcataga gagactaaat agcaatattg tataggttat
     8401 tttaggagtg ctttcgcctg ttttaggaag cttgtttgtc tttacctttt gattatcggt
     8461 ttggctagtc acccctaaga cacgcttctg agcaacctca ctttctttag tcatgccttt
     8521 tttgaatatc aacgcctggg gctgatttgg cggattgata ggtgtggtat tgagagagtc
     8581 attatcattg agtgattgac tataattctc tgcggaagaa actctatcac ggccatcaag
     8641 ctcttgttcg gttgctttta tttggtttaa taactcttca acttgagctt gtgtagcttg
     8701 tctattggaa agcagcaatt ctgctttctt gataaggtta ttataatttt ctttagcaaa
     8761 ttgactggcg ttctgatatt tagatgatgt ttgtttcaga acgtttgctt taattatcat
     8821 atggtgtaaa ccagagtaat cagtttcatg accatttaat tgatttttag caatatcaag
     8881 attttctaag gctttgtcta cttctgattg agtgacgtta ggtttttgta gaaggttttt
     8941 cgccaattgg acttgattga tataagttga ttggcttaat ggactagcat taacaaattg
     9001 atagtttccc ttgatttctg tttcagctaa aatagctttt gccagtaaag aagtatctgt
     9061 ttctttaccg ttaagagctt ggcgactttc tctcaaagca gcaatagcat tatcaatact
     9121 tgtttgttcc actctatttt taattaaagt ttgtgccttt tcaagattag ctaggtaatt
     9181 attttttaag tgagttccag cattataaaa cacaggctgg gtaataatat cagtttgttc
     9241 tgctagggca ttaatcagag gagctttatt aatcagcgtc aattttgtta ggtttacctg
     9301 atcttctttt acggatacaa gtaaatcatc taagttctct aaagtgctat acccactagg
     9361 cagcgttacg tttaaacgat agtctcctac aggtatgttt ttaccatagt cagtaggaga
     9421 ataagttgct tttggtagat caacaacagt atttgtctta gtaaccaact ggactgttgc
     9481 tccttttggt aaaagcttgt caaattcaac gagtaaggct gctttcttga gtaagttaac
     9541 tttaaataag acgtctttaa ggctatcttt ttcagaaata gttacagtga cacttttggg
     9601 actgattaga tttgctcgtt cctcatcgta taagaataga tcaaacgtat aggtaccaaa
     9661 aggcaatttc aataaattga ggtctttccc gtgatgttgc tttttgatga tattaccttt
     9721 gtcatctttg acagagtaag agaaatcaat atcgacatta ctgttaagct cagggctaaa
     9781 cactttgacg tttactaaac catttttatt gccaatattg atcaaatctt cgagttttgc
     9841 tgatactaga ttgcctgcat aatcttcaac agtatagtag acatgagaga aatcgttaac
     9901 ctctttaggt agaataaagc taccatcctt ttcttgtttg ataaatactt tgttatcttc
     9961 aacaaggata ccgtcttctc cttgttgacg taagacgcta ttataatgac catctttatc
    10021 tttttttaag taaaaaactt gttctctaaa gataccagat tccccgtgtt ctactgcagg
    10081 acgagctttg aagatacgtc tatctttgtc ataagttgct gttgttagtg taggatcttg
    10141 tctatccaaa gtgatatcaa acaccatttg ttgtggctta gagccaggga catcagagta
    10201 ataagttaaa acgtatttgt attttccatc ttctaaagga ataccatcag tagtggttcc
    10261 tttccattct gtgtcatata aaaatgtaga ttttggattt tcagtatttc cgctgtagta
    10321 atttttatct cctgcttggg gagcacttac ccatagtggt ttttgtaaat taacatcatc
    10381 tgcacgatag actttggctc ttaaattatt gaaattacgt aagaaaacac ctttcactgc
    10441 aacggcatct tgattttggt catcattagg tgaaatggct agatgaggct ggcctttttg
    10501 atctagttgt aagatccatt ttccatctat agacttatag cttcccaagg ctttcaattg
    10561 gataggacta gtaccgtctg ttgagtagat aggctctgaa cttgtagtca ttaaggcagt
    10621 ataatcttct gaaccaggaa cttcgtttgt ttgttttggt tggaaataaa atcccttttc
    10681 tttgttagca acaagcttat aaatggattt ttctaaaact tctaagtttt ggaattctcc
    10741 cttaaatcca acataaggaa tactaagaac ttccccacca tcaacagggt ctgtaaaacg
    10801 tacgtagcct tctaggaaat agccgtttgg cattactttt tttaacatgt catggtattt
    10861 cgaaacatct atatcaattg taatcgtttg ggtttgccct ggttcaatcc gtatcgtttt
    10921 ccctgtaaat gtaccaagtt gttgaggagc caatgtgaca aagccatctt taacttgatc
    10981 agtatttaaa taggttgtat agtgtaaatc ttttgcaact ttattgatat tatgaacagt
    11041 aacatcaaag gaaattttat ctttaatatt tcctaatgta acgctgccat agttgttttc
    11101 cccaccagtt aggtataatc ctgtctgaac tgctgctgcg acatcgataa tacctgctcc
    11161 ttgttggcgt ggtgaggtgt aagcgcctgt atctttgttt aggtgtgctt tagcagtact
    11221 cataagaagg tatttgacag ttctttcaat gtcacctttt ttaagttctg gatgttcttt
    11281 caataagtat tgtttaacta gagcagtagc accagcgaca tggggagaag ccatacttgt
    11341 cccactcatc atatcatatt cattatcatt gatagcggcg taaatagagc ctccaggagc
    11401 agataagtca ggttttaatt gtccgtctgc tgttaacccc cagcttgaaa agtcagatag
    11461 gacacctgca ttagggttgg cttgtttatt tttgatattg ttaaatacaa ttttatagtt
    11521 gtttttagct aaaacatctc caaactcttt ttgggtaaaa atagcaggaa tagcacttgc
    11581 ttcaggatct aaactcattg ttagattagc ttccccagct ttgttattaa agataatagc
    11641 tcccacagca ccgtgattaa tagcattgac gacttttttt gtaaaagtaa tatctccacg
    11701 ttcaattaag gcgattttac catttaaagt tttgtcctta taatcattgt cgtttccttt
    11761 tcctacgaaa acataatcat attgtttccc tacttcaaag gtcttatcac taactttagg
    11821 atctgcataa gcagctaatc cgttgttgag atttctgttg ttttctaatc cgataatgtt
    11881 gaagacttta ttaaccagtg ttgtattatt atatgatgct acagaaatgg cctcacgagc
    11941 tgttgatgga ctaccaacta aaccataatc aggataaaga gcagaaggct ttgatgctcc
    12001 actcccaaat gtaccgtcgt tacctgctgc tataacaaca gaaacgccag cgagtctagc
    12061 catctctaaa gcttttataa gtcggtcatc ggcattaact aaagacccat tagctccacc
    12121 taaacttaaa ttaatgctgt cagcaccgag tttaacggca tcttcaatag ctttaacata
    12181 aagggctggt ccagttcctc ttttttcatc agagaagact ctcataaaca ttacttgtgc
    12241 ttcaggagca acaccataga ttaattcatt tgtatctttc ttgctaggat tagctgttgc
    12301 gatactggtt acgtgcatac catgagaagt gcttttcacc tcttttagct ctgtattgac
    12361 atcaacatag ttgtgaccaa agattacttt gttgttatac cattttccat agtttatacc
    12421 agcttttgct ttagcagcat tcatttgctg ttcgttttgg tattttgctt ttgtcgaatc
    12481 atttaattgg agagcatcgt gattgatatc tagtcctgag tcaataattg ctacaacagt
    12541 tccctctcct ttgtagcctg tgttccatac tttggggaca gtgataattt tattactatc
    12601 aatgttttga ggggtttctt tagaaatagc tggtgtagat ttgactgcta aattttgttt
    12661 ggttaaagga gttttagaaa cttcttgagt gctttcaacc ggttgatttg cgactgggga
    12721 aaatgtctcg gtggcttgag agtgagactc agctactggt tgtgtttctg ttggactatt
    12781 attagccttt gtaacaagat tgtcttgagc tgtcgacgca agggctgatg acgtgttgtt
    12841 agtaggtgtc tcatttgcta tgctcgttgg actgtctttt acttgctgtt caactgcaat
    12901 atcagatggc ttatttatga cagaatcagc agctacattt gtgataccac ctaaaaaaaa
    12961 tagggttccc aaaagtacag atacggcacc taacttatat ttccggattg aaaaacgctg
    13021 ttttgtgttc ataaaaatct ccttttcttg taacataata attacaaaaa ctattaaaac
    13081 ataaaaaatc aaaattttct acaattttct gaattttcat tacaaattat tttgtggtaa
    13141 atttaagacc gtacctaaag cattttttaa aaatcattct atgaaaataa taatattgtc
    13201 caactatatg gctacaatat caaaggttta tagaataatt acaggatatt tattagtcta
    13261 attgctggca aagataaata atcattcctc ttctttctta gtaatcaccg ttgtgctata
    13321 ataactgaat atactgaaaa tttaggggga tatgatggaa aaaggattta aagagggagt
    13381 gaaagatgcc ttgcccacag ctttagggta tatctcaata ggcttggcgt ttgggatagt
    13441 tgcttcagca tcagatttat ctgcaataga agttggtctt atgagtgcct tggtatatgg
    13501 gggatctgct cagtttgcta tgtgtgcgct attattagca aaagcagact tgatgactat
    13561 tacgatgaca gtatttttag ttaatttgag aaatatgtta atgagtttac acgcgactac
    13621 tatcttcaag tctgctcacc taatgaatca attagccata ggaactctaa taacagatga
    13681 gagctacggc gttttacttg gagaagcatt acatcacaaa gttgtatcgc ctagttggat
    13741 gcatggtaat aatgtgatga gttatttgac atgggtaatc tcaacgataa taggaacttt
    13801 actggggagt actattccta atccagaaat gtttggttta gactttgcat tagtcgccat
    13861 gtttataggt ctctttgtat ttcagttgtt tgggatgtta tctgatggaa agcgacttgt
    13921 tgtatatgtt cttgcttctg taggtttaag ttacttctta ttagctactt ttttgtcagg
    13981 agccttatca gtattgcttg cgactgttgt tggatgtagt gtgggagttg ttttagatga
    14041 taaatagtac cgtttttctt gctattcttt taggttttgt tgtttcttgg gtgccaagag
    14101 tcgctccatt catatttgta aaatacaaag ggctaccaaa tagtcttgtg aaatttttaa
    14161 aatttcttcc tctttctatt ttatttgccc taacattctc tagctttttt ttagttaaat
    14221 taggacaatt gccaacacta aaatggttag atttattggc tggcatacca actctagttg
    14281 tcactattcg atatcgaaat ttattttaca ctgttcttac tggtatcgtt actatggctt
    14341 tattgagatt actattttaa cgtgtaggaa tggaagatta ataaaaacac aaaaaacgca
    14401 tctatttcta gatacgtttt ttgtgttttt gtctagacag gaactttt
//
LOCUS       DATRTN010000041        14157 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.041, whole genome shotgun sequence.
ACCESSION   DATRTN010000041 DATRTN010000000
VERSION     DATRTN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14157
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.041"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            150..2258
                     /locus_tag="VBE22_001212"
     CDS             150..2258
                     /locus_tag="VBE22_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO7202678.1"
                     /translation="MANNNFYNRDPFGNMDDIFNSLMGNMGGYNSENKRYLINGREVT
                     PEEFSQYRQTGKLPGQELNNQNTPTNQVSADSVLTKLGTNLTDQARQHLLDPVIGRNK
                     EIQETAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIINGDVPAAIKNKEIISIDIS
                     SLEAGTQYRGSFEENIQNIIKEVKETGNIILFFDEIHQILGAGSTGGDSGSKGLADIL
                     KPALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPTAQDTFNILMGIRNLY
                     EQHHNVVLPDSVLKAAVDLSIQYIPQRSLPDKAIDLIDMTAAHLAAQHPVTDLKSLEK
                     EIAAQRDKQEKAVNTEDFEEALKVKTRIEELQNQIDNHTEGQKVTATINDIAMSIERL
                     TGVPVSNMGASDIERLKELGNRLKGKVIGQNDAVEAVARAIRRNRAGFDDGNRPIGSF
                     LFVGPTGVGKTELAKQLAFDMFGSKDAIVRLDMSEYNDRTAVSKLIGATAGYVGYDDN
                     SNTLTERIRRNPYSIVLLDEIEKADPQVITLLLQVLDDGRLTDGQGNTINFKNTVIIA
                     TSNAGFGNEAFTGDSDKDLKIMERISPYFRPEFLNRFNGVIEFSHLSKDDLNEIVDLM
                     LDEVNQTIGKKGIDLVVDENVKSHLIDLGYDEAMGVRPLRRVIEQEIRDRITDYYLDH
                     TDVKHLKANLQDGQIVISER"
     gene            complement(2352..3296)
                     /gene="mmuM"
                     /locus_tag="VBE22_001213"
     CDS             complement(2352..3296)
                     /gene="mmuM"
                     /locus_tag="VBE22_001213"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEO7202679.1"
                     /translation="MGRFKELLESKKALILHGALGTELESRGCDVSGKLWSDKYLIED
                     PAAIQTIHEDYIRAGADIVTTSTYQATLQGLAQVGVSESQAEDLIRLTVQLAKAVREQ
                     VWKSLTKEEKSERIYPLISGDVGPYAAFLADGSEYTGLYDIYKEGLKNFHRHRIELLL
                     DEGVDLLALETIPNAQEAEALIELLVEDFPQVEAYMSFTSQDGKTISDGSAVAGLAKA
                     IDVSPQVVALGINCSSPSLVADFLQAIAEQTDKPLVTYPNSGEIYDGASQSWQSSRDH
                     SHTLLENTSDWQKLGAQVVGGCCRTRPADIADLSEHLT"
     gene            complement(3301..4677)
                     /locus_tag="VBE22_001214"
     CDS             complement(3301..4677)
                     /locus_tag="VBE22_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO7202680.1"
                     /translation="MENHRYEDEGKFQRKMTSRHLFMLSLGGVIGTGLFLSSGYTIAQ
                     AGPLGAVLSYLIGAVVVYLVMLSLGELAVAMPVTGSFHTYATKFISPGTGFTVAWLYW
                     ICWTVALGTEFLGAAMLMQRWFPDVPAWAFASFFALVIFGLNALSVRFFAEAESFFSS
                     IKVIAIIIFIILGLGAMFGLVSFEGQHKAILFTHLTANGDFPNGIVAVVSVMLAVNYA
                     FSGTELIGIAAGETDNPKEAVPRAIKTTIGRLVVFFVLTIVVLASLLPMKEAGVSTAP
                     FVDVFDKMGVPFAADIMNFVILTAILSAGNSGLYASSRMLWSLANEGMLSKSVVKINK
                     HGVPMRALLLSMAGAVLSLFSSIYAADTVYLALVSIAGFAVVVVWLAIPVAQINFRKE
                     FLKENRLEDLSYKTPFTPVLPYITIVLLLISIVGIAWDSSQRAGLYFGVPFIILCYIY
                     HKLRYKTW"
     gene            complement(4807..5457)
                     /locus_tag="VBE22_001215"
     CDS             complement(4807..5457)
                     /locus_tag="VBE22_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEO7202681.1"
                     /translation="MSYFRNYWYRFGAILFIILAVILLVFRPDWSMLHYLLYFNFMAL
                     LAHQFEEYQFPGGASPIINYVVYDEEELMDRFPGNTQSIMLVNTIAWLLYIASIAFPQ
                     AYWLGLGVMFFSLTQLLGHGFQMNIKLKTWYNPGLATTVFLLVPIACAYIYQASAEGM
                     LTWGDWLGGFIMLIVCVLTSIIAPVQLLKDKKTNYIISPWQMDRFHKVINFVRIKK"
     gene            complement(5469..6118)
                     /locus_tag="VBE22_001216"
                     /pseudo
     CDS             complement(5469..6118)
                     /locus_tag="VBE22_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645479.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     gene            6210..6839
                     /locus_tag="VBE22_001217"
     CDS             6210..6839
                     /locus_tag="VBE22_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO7202682.1"
                     /translation="MTESVTVNKKRIKQITQTQEDILTAFGQLLEEYPYEKIQISQIA
                     KKSGYARRTLYRHFDSRDDLLTLFIERLTLNLFKQLGQLEQATFSQVFQNSFSYWSDY
                     KSLLLILRKNDLLPQFQQSWFRHIDLIELGRGDLSSNTYAQRFAIGGIFSVLIEWIHQ
                     DCQTSIEELTQVSFDIINHLKNKKELCSSLNSYCKALFVINSQIITQIK"
     gene            complement(6820..7416)
                     /locus_tag="VBE22_001218"
     CDS             complement(6820..7416)
                     /locus_tag="VBE22_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEO7202683.1"
                     /translation="MAEEFLNTHNASILLSAANKSHYPQDDLPEVALAGRSNVGKSSF
                     INTLLGRKNLARTSSKPGKTQLLNFYNIDDKLRFVDVPGYGYAKVSKTERAKWGKMIE
                     EYLVTRDNLRVVVSLVDFRHDPSADDIQMYEFLKYYEIPVIIVATKADKIPRGKWNKH
                     ESSIKKKLNFDKKDHFIVFSSVDRTGLDESWDTILSEL"
     gene            complement(7427..8653)
                     /gene="clpX"
                     /locus_tag="VBE22_001219"
     CDS             complement(7427..8653)
                     /gene="clpX"
                     /locus_tag="VBE22_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEO7202684.1"
                     /translation="MAGNRNNDMNVYCSFCGKSQDEVKKIIAGNGVFICNECVALSQE
                     IIKEELAEEVLADLAEVPKPKELLEILNQYVVGQDRAKRALAVAVYNHYKRVSYTESS
                     DDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENIL
                     LKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIQINTKNILFIVGGAFDGIEDLVKQRLGEKIIGFGQTSRKIDD
                     NASYMQEIISEDIQKFGLIPEFIGRLPVVAALELLTAEDLVRILTEPRNALVKQYQTL
                     LSYDGVELEFDQDALLAIADKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVRI
                     TKAAVEGTDKPVLETA"
     gene            complement(8871..9365)
                     /locus_tag="VBE22_001220"
     CDS             complement(8871..9365)
                     /locus_tag="VBE22_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO7202685.1"
                     /translation="MTKQIIAIWAEDEDHLIGVNGGLPWRLPKELHHFKETTMGQALL
                     MGRKTFDGMNRRVLPGRETIILTKDEQFQADGVTVLNSVEQVIKWFQEHNKTLFIVGG
                     ASIYKAFLPYCEAIIKTKVHGKFKGDTYFPDVNLSEFKVISRDYFEKDEQNAHAFTVT
                     YFEK"
     gene            complement(9445..10284)
                     /locus_tag="VBE22_001221"
     CDS             complement(9445..10284)
                     /locus_tag="VBE22_001221"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEO7202686.1"
                     /translation="MTKADLLFKDNITKIMSEGVFSEQARPRYKNGEMANSKYITGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEIFWIYQDQTNDLVVLNDKYGVTYWNDWEVGHT
                     GTIGQRYGAVVKKHNIISKLLKQLEDNPWNRRNVISLWDYEAFEETEGLLPCAFQTMF
                     DVRRVNGELYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWRVGKFFYFINNL
                     HIYDNQFEQAQELLKRQPSECNPKLVLNVPDGTDFFDIKPDDFALVDYDPIKPQLRFD
                     LAI"
     gene            10333..11610
                     /locus_tag="VBE22_001222"
     CDS             10333..11610
                     /locus_tag="VBE22_001222"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEO7202687.1"
                     /translation="MRNKTLKVKAFFHIFSFFMVKWNLAKMTFIQKGLPMKIGIDKIG
                     FATSQYVLEMTDLAIARQVDPEKFSKGLLLDSLSITPVTEDIVTLAASAANDILSDED
                     KETIDMVIVATESSIDQSKAASVYVHQLLEIQPFARSFEMKEACYSATAALDYAKLHV
                     EKHPDSKVLVIASDIAKYGIKSTGESTQGAGSIAMLISQNPSILELKEDRLAQTRDIM
                     DFWRPNYSDVPYVNGMFSTKQYLDMLKTTWKEYQKRFNTSLSDYAAFCFHIPFPKLAL
                     KGFNKILDNNLDEQKKAELQENFEHSITYSKKIGNCYTGSLYLGLLSLLENSQNLKAG
                     DQIAFFSYGSGAVAEIFTGQLVDGYQNKLQSDRMDQLNKRQKITVTEYEKLFFEKTIL
                     DENGNANFNTYRTGTFSLDSICEHQRIYKKINN"
     gene            11612..12895
                     /locus_tag="VBE22_001223"
     CDS             11612..12895
                     /locus_tag="VBE22_001223"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEO7202688.1"
                     /translation="MTKISWTGFSKKSPEERIHYLEEQDFLADSSLEIVTNQDLLSLS
                     LANQMAENVIGRIALPFSLVPDVLVNGKVYQVPYVTEEPSVVAAASFAAKIIKRSGGF
                     LTTVHNRKMIGQVAIYDVQDSQHTKESILNQKQQLLEIANAAHPSIVKRGGGACDLTI
                     EIKEDFLIVYLMVDTKEAMGANMVNTMMEALSSPLEDISKGKSLMSILSNYATESLVT
                     ATCRVDLRFLSRQKEEAIKLAQKMTMASQLAQVDPYRASTHNKGIFNGIDAIVLATGN
                     DWRAIEAGAHTYAVKDGQYRGLSKWSYKVDDNFLEGTLTLPMPVATKGGSIGINPSVQ
                     LAHDLLGRPNAKELASIVLSIGLAQNFAALKALVSTGIQAGHMKLQAKSLALLAGAKE
                     EQISEVVKQLLDSKHMNLETAQKIVNKLTKSKKRI"
     gene            12987..13436
                     /locus_tag="VBE22_001224"
     CDS             12987..13436
                     /locus_tag="VBE22_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEO7202689.1"
                     /translation="MNKNQYPKWEELPELDLYLDQVLLYVNQLINPKTITNDKLLTAS
                     MINNYVKHNYISKPIKKKYNRRQVARLIVITAFKQVFAIQEISQTLEILTADNHSEEA
                     YNGFAACMNKEEVHDLPPVVISACQTLNLYQETQKLVLELGGKNKNA"
     gene            13429..14085
                     /locus_tag="VBE22_001225"
     CDS             13429..14085
                     /locus_tag="VBE22_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEO7202690.1"
                     /translation="MLKEMSIKLSPQLSFGEELANSITHAVGALLMLILLPITAVYSH
                     NHFGLQAALGTSIFVTSLFLMFLSSSIYHSMTYSSLQKYVLRMIDHSMIYIAIAGSYT
                     PVALSLIGGWLGYLIIFLQWGITLFGILYKIFAPKINDKFSLVLYLIMGWLVIFIFPA
                     IITKTGPAFWGLLLAGGICYTIGALFYARKRPYDHMIWHLFILLASILQYIGIVYFML
                     "
BASE COUNT         4728 a         2788 c         2471 g         4170 t
ORIGIN      
        1 gtatttattt attgaccttc gctgaccaaa ggtgtattat agtaccagaa agataattaa
       61 atatttaaat tagacctctc tatatgtatt aaagtatagg aatcaataat taactttcaa
      121 aaattaaaat caattgaggt atttaatata tggcaaataa taatttttat aatcgtgacc
      181 cttttggaaa tatggatgat atttttaatt ctctaatggg aaatatgggg ggctataata
      241 gtgagaataa acgatactta atcaatggac gggaagttac tcctgaagaa ttcagtcaat
      301 atcgtcaaac tggaaaactc cccggtcaag agctaaataa ccaaaatact cctacaaacc
      361 aagtctctgc agatagtgtt ttgactaaat taggaactaa tttaaccgat caagcacgtc
      421 agcatttgct agatccagtt attggacgaa acaaagaaat tcaggaaact gcagaaattc
      481 tagctcgtcg tactaaaaat aatcctgttc tagttggtga tgcaggtgtt ggtaaaacag
      541 ctgttattga aggtttggca caagcaatta taaatggtga tgtccctgct gctatcaaaa
      601 ataaagaaat catttcgata gatatctcta gccttgaagc tggcacccaa tataggggta
      661 gttttgaaga aaatattcaa aatatcataa aagaagttaa ggaaacagga aatattattc
      721 tcttctttga tgaaatacac caaatattag gagccggttc aactggtggt gatagtggct
      781 ctaaggggct cgcagatata cttaagcctg cactttcacg tggtgaatta acggttatag
      841 gtgcaactac tcaagatgaa tatcgtaaca ccattctcaa gaatgctgcc ttagcacgcc
      901 gctttaacga agttaaagtc aatgcaccta ctgcacaaga tactttcaat atccttatgg
      961 ggataagaaa cctctatgag caacatcata atgttgtttt acctgactct gttttgaaag
     1021 cagctgtaga tttatctatt caatacatac cacaaagaag tctccctgat aaagcaattg
     1081 accttattga tatgactgct gcacacctag ctgcgcaaca tcccgtaact gaccttaaat
     1141 ccttagaaaa agaaatcgct gcgcaaagag acaagcaaga aaaagctgtc aatactgaag
     1201 attttgaaga agctcttaag gtcaagacac gcattgaaga gttacaaaac cagattgata
     1261 atcataccga aggacaaaaa gtcactgcaa ctatcaatga tatcgctatg tcaattgagc
     1321 gtctaactgg agttcctgta tcaaatatgg gtgctagtga tattgaacgc ttaaaagaac
     1381 ttggcaatcg tctaaaaggt aaagtcattg gtcaaaatga tgctgttgag gcagttgcaa
     1441 gagctattcg ccgaaatcgt gctggtttcg atgatggtaa tcgtccgatt ggtagctttc
     1501 tatttgttgg accaactgga gttggtaaaa ctgagctagc aaagcaatta gcttttgata
     1561 tgttcggatc aaaggatgct atcgttagac ttgatatgtc tgaatataat gatcgtactg
     1621 cagtttctaa gctaattgga gcgactgctg gttacgttgg ttatgacgac aatagtaata
     1681 cactaacaga acgtatccgt cgaaatcctt attctattgt tcttcttgat gaaatcgaaa
     1741 aagctgaccc gcaagttatc acgcttctcc ttcaagttct agatgatggt cgtttaactg
     1801 atggtcaagg aaacactatt aactttaaaa atactgttat cattgcaacc tcaaatgcag
     1861 gttttggaaa cgaagcattt acaggtgaca gcgataaaga cttgaaaatt atggaacgaa
     1921 tttctccata tttccgtcca gaatttctaa atcgtttcaa tggtgttatt gaattctctc
     1981 acctaagcaa agatgactta aacgaaattg tagatttgat gcttgatgaa gttaaccaaa
     2041 caattggcaa aaaaggaatt gaccttgtgg tagatgaaaa tgttaaatca cacttaattg
     2101 acctgggtta tgacgaagca atgggagtac gtccattgcg ccgtgtcatc gagcaagaaa
     2161 ttcgagatcg catcacagac tactatctcg atcatacaga tgttaaacac ctaaaagcta
     2221 atttgcaaga tggccaaatc gtcatttctg aaagataaaa acaaaacccg tttggaagaa
     2281 tccaagcggg ttttctagtg gattgaaaaa gggaatcaga acttatcaaa atcttcttta
     2341 attcacttta cttatgtcaa atgttctgag aggtcagcaa tatcagctgg tctagttcgg
     2401 caacaacctc cgacaacttg ggcgccaagt ttttgccagt cacttgtatt ttcaagtagg
     2461 gtgtgtgaat gatctcggct agattgccaa gattggcttg ccccatcata aatctctcca
     2521 gagtttggat aggtcacaag aggcttgtcc gtctgctctg ctattgcttg caagaaatca
     2581 gcaactaacg atggacttga acagttaatg cccagcgcca cgacctgtgg gctaacatca
     2641 atagctttcg ccaagcctgc aacagcacta ccatctgaga tggtcttacc atcttgagag
     2701 gtgaatgaca tataagcctc aacctgaggg aaatcctcga caagtagctc aataagagcc
     2761 tcagcctcct gagcatttgg aatggtttca agtgccagaa ggtcaacacc ctcatctaaa
     2821 agaagctcaa tacgatggcg atggaaattt ttgagacctt ccttataaat atcgtagagc
     2881 cctgtgtact cagaaccgtc cgccaaaaag gcagcgtagg gaccaacatc cccagaaatc
     2941 aaaggataga ttctttctga tttttcttct tttgtcaaac ttttccaaac ttgctcacgc
     3001 acagcttttg ccaactgaac agtcaagcga atcaagtctt ctgcctgact ttcagagaca
     3061 ccaacttgag ctagcccttg taaagtcgcc tgataagttg atgtcgtcac aatatcagcg
     3121 ccagcacgaa tataatcttc atgaatggtc tgaatagctg ctggatcctc aatcagatat
     3181 ttgtctgacc aaagtttacc agaaacatca caacctctac tctcaagctc ggtccccaag
     3241 gcaccgtgta ggatgagagc ttttttactc tctaaaagtt ctttaaatct tcccataaga
     3301 tcaccatgtc ttgtaacgta gtttgtgata aatataacat aaaataataa atggaacacc
     3361 gaagtaaagc ccagcacgtt gtgagctatc ccaagcaatc cccacaatcg aaatcaaaag
     3421 caaaacgatt gtgatataag ggagaactgg tgtaaagggt gttttgtaag acaaatcttc
     3481 tagtcgattc tctttcaaga attccttgcg gaaattgatt tgtgcgaccg gaatggctag
     3541 ccatacgaca acaacagcaa agcccgcgat tgaaaccaag gctagataaa ctgtgtctgc
     3601 agcgtaaata cttgaaaaga gcgacagcac tgctcctgcc attgacaaga gaagagcacg
     3661 cattgggaca ccgtgtttat tgattttcac gacagatttt gacaacatac cttcattggc
     3721 aagggaccag agcatacggc ttgatgcgta gagacctgag ttaccagcag acaggatggc
     3781 tgtcaagata acaaagttca tgatatccgc cgcaaaaggg actcccatct tgtcaaagac
     3841 atcaacgaat ggtgctgtgg atacgcctgc ctctttcatt ggcaatagcg aagctaggac
     3901 aacaattgtc agtacaaaga aaacaaccaa gcgaccgatt gtcgttttaa tagcccttgg
     3961 cacagcttct tttggattat ccgtttcacc tgccgcaata ccaatcaact cagtaccaga
     4021 gaaggcatag ttaacagcca acatgactga gacaactgca acgataccat ttggaaagtc
     4081 accattggca gtcagatgag tgaaaagaat agctttgtgc tgaccttcaa aggaaactag
     4141 accaaacata gcacctaagc ccaagataat aaagataatg atagcaataa ccttaatact
     4201 tgagaagaaa gactctgctt ctgcaaaaaa gcgcacactg agagcattta agccaaaaat
     4261 cacaagggca aagaaagaag caaatgccca agccggcaca tctgggaacc agcgctgcat
     4321 gagcatggca gcacctaaaa attcagttcc caaggcgacc gtccaacaaa tccaatatag
     4381 ccaggcaaca gtaaaacctg tgccaggact gataaactta gtggcataag tgtggaatga
     4441 ccccgtcacc ggcatggcaa ctgccaattc accaagtgat agcatgacca aataaaccac
     4501 aacggcacca atcaaataag acagcacagc tccaagcgga ccagcctgtg caatggtata
     4561 acctgaactc aagaaaagcc cagtcccgat aacaccacct agagataaca taaagagatg
     4621 acgactggtc atcttacgct ggaatttacc ttcatcttca taacgatgat tttccataac
     4681 aacctccaag aaactaatac ttttatccta ccacaatcta gtcgtattgg ggaataggta
     4741 ttagctatca tatatgataa agaaaaacta ttacaaaaaa tcagcgaatc ataaatagcg
     4801 gtaaccttat ttttttatcc ttacaaaatt aatgacctta tgaaaacggt ccatttgcca
     4861 aggactaata atataattgg tcttcttatc cttcaatagc tgtacaggtg caataatact
     4921 agttagtaca cagacaatca acatgataaa accacctagc caatctcccc aagtgagcat
     4981 tccttctgca ctagcttgat agatgtatgc gcaagctatt ggtactagga gaaatactgt
     5041 cgttgctaga ccaggattat accaagtttt aagtttaata ttcatctgaa aaccatgacc
     5101 caagagctgc gttagactaa agaacatgac tcctaatcca agccaataag cttgaggaaa
     5161 agcaatacta gcaatgtaaa gcaaccaagc aatagtatta accaacataa tagactgagt
     5221 attgcctgga aaacgatcca tcagctcttc ttcatcataa acaacatagt taatgatagg
     5281 tgatgcccca ccgggaaact gatattcttc aaattgatgc gctagaagtg ccataaagtt
     5341 aaaatacaat agatagtgaa gcattgacca gtcaggtcta aaaacaagca atattactgc
     5401 taaaataata aataaaattg ctccaaaacg ataccagtaa tttctaaaat atgacatgat
     5461 aatacctcct atttaaactg tgtttcacga aaatgtgtta aaaatggttt tgagcgtgaa
     5521 agatcaacat cactaaatgc gtaatttgaa tgtcgaccta aacgcttata gattggggaa
     5581 cgaaaggcta taaagtagtt aagaacaatc catatcagcc taaaaaccaa tcaagtccag
     5641 aatacaactt ccaatcatga gctaaataat agacagaaac agggacaaga cctactaagg
     5701 tcgtaagaag tcctggatta taccacttct tcaaggaaag attaaaaaag aaaagatgca
     5761 tggcaagttc tgcaaatgca aaaatcatga ctgcaagtgt taaaaatggt atattgggac
     5821 aaaaaatcgg caaaagataa acaatcactg caaaccactg attcccgaaa aaggctgata
     5881 gatggtttaa gtcccactta ctagtatcag tttcaaccga tttaagttca actagcatag
     5941 cgataagcgg aaaacctcca gggaaaccaa attcttcgaa aaagtgtaat taaataaaaa
     6001 gtcccgatat aagtagtgac tgcactcgta aatcccaatt tccaaaaaat aatgctaagg
     6061 caaaaagaga ggcaagatag ggacaaaaga ggtaccattt tgttatcaat gttttcataa
     6121 gatgacctcc aagatttgta acatttgata ccatatagta taatcaagtg aacctataat
     6181 atcaatacct ttggaggttt tatgcacaaa tgactgaaag tgtgacagtt aataaaaaga
     6241 gaataaaaca aatcactcaa acacaagagg atatcttaac agcttttggc caacttttgg
     6301 aagagtatcc ctatgagaaa attcagatca gccaaattgc caagaaatct ggctatgcta
     6361 gaaggaccct ttaccgacac tttgacagtc gagatgacct acttaccctt ttcatcgaaa
     6421 gactaacatt aaatctcttt aagcaacttg gtcaattgga acaagctact tttagccaag
     6481 ttttccagaa ttcttttagt tattggtctg attataaatc ccttcttctc attcttagaa
     6541 agaatgacct actccctcaa tttcaacaat cctggttccg acatattgat ttgatagagt
     6601 taggtcgtgg tgatttatct tccaatacct atgctcagcg atttgcgatt ggtggtattt
     6661 tcagtgttct aattgagtgg attcatcaag actgccaaac aagtatcgaa gaattaactc
     6721 aagtttcctt cgatattatc aatcatttaa aaaataaaaa agagctttgc agtagtctca
     6781 acagctactg caaagctctt tttgtcataa actctcaaat tataactcag ataaaatagt
     6841 atcccatgat tcatcaagac ctgtgcggtc aacagatgaa aagacgatga aatggtcctt
     6901 tttatcaaag tttaatttct tcttaataga cgactcatgt ttattccact tgccacgagg
     6961 tattttatct gcctttgtcg ctacaataat tactggaatt tcatagtact taagaaattc
     7021 atacatttga atatcgtccg cacttggatc atgacgaaaa tcaactaaac taacaacaac
     7081 acgaaggtta tcacgtgtta caaggtactc ttcaatcatc ttgccccact tagcacgctc
     7141 agttttagac accttagcgt agccatatcc agggacatcg acaaaacgca gtttgtcatc
     7201 aatgttataa aagttgagta gttgtgtctt acctggctta ctcgacgtac gcgctaggtt
     7261 tttacgacca agaagcgtgt tgataaagct tgatttacca acatttgaac gaccagcgag
     7321 agcaacttct gggagatcat cctgaggata atgtgacttg ttagctgcac tcaataaaat
     7381 agacgcatta tgagtattta aaaattcttc tgccatctct atctccctaa gccgtctcta
     7441 aaacaggctt gtcagtaccc tcaacagcag cctttgtgat acgaactttt gttacatctt
     7501 cttggcttgg aatttcaaac atgatatcaa gcatcgtttc ttcaataata gaacgtaaac
     7561 cacgtgcacc agtcttgcgc tcgatagcct tatcagcgat agccaataga gcatcctgat
     7621 caaattccaa ttctacacca tcataagata ataaggtttg gtattgttta accaaagcat
     7681 tgcgtggttc tgtcagaata cgaaccagat cttctgcagt aagaagttct aacgctgcaa
     7741 ctactggtaa acggccaata aactctggaa tcagtccaaa cttttgaata tcctcagaaa
     7801 ttatctcttg catataagaa gcgttgtcat caattttgcg gcttgtctgt ccaaaaccaa
     7861 taattttttc gcctaaacgt tgcttcacaa ggtcttcaat accatcaaaa gcaccaccga
     7921 caataaaaag gatgttcttg gtattaattt gaatcatttc ttggttagga tgtttacgcc
     7981 caccctgtgg gggaacactt gctaccgtac cctcaataat tttaagaagg gcttgctgta
     8041 caccttcacc agatacatca cgtgtgatag aaacattttc gcctttctta gcaattttat
     8101 ctatttcatc aacgtagata ataccacgct cagcacgttc gacattataa tcagcagctt
     8161 gaatcaattt aagaagaata ttctcaacat cttctccaac gtatcctgct tcggtcaatg
     8221 aagtcgcatc tgcaatagca aacggtacat taaggctttt agctagtgtt tgtgctaaga
     8281 aggtttttcc tgagccagtt ggaccaatca tcaaaatgtt ggatttttgc aaatctacat
     8341 catcgtcact actctcggta taactaacac gcttgtaatg attgtagaca gcaactgcta
     8401 aagcacgttt agcacgatct tgccctacaa catattgatt taatatttct aatagttcct
     8461 taggttttgg tacttctgct aaatcagcaa gtacttcctc agctaattct tccttaataa
     8521 tttcttgtga taaggccaca cattcattac aaatgaaaac accattacct gcaataattt
     8581 tttttacttc atcttggctt ttgccacaaa atgaacaata gacattcata tcgttattac
     8641 gattcccagc cattttttcc tcaacttttc tattcattta atttaaaata aggttccata
     8701 aaaggcatgg attgtgttga caaggttagt gaaagcattc atcaaaaaaa ccgttcctaa
     8761 tccatatctt tttttcttta tagcaggaat tgcacataaa acacaaatca cagctaaaaa
     8821 tgcggtaaag accccaaaaa ggctacgttg cattgaatag tattccttcc ttatttttca
     8881 aaatatgtca cagtaaaagc atgagcattc tgctcatcct tttcaaaata atctctagag
     8941 ataactttaa actcagatag attaacatca ggaaaatagg tatcaccttt aaattttcca
     9001 tgaactttag tttttatgat tgcttcacaa taaggcagaa atgctttata aatacttgca
     9061 ccacctacaa taaataaggt cttattatgt tcctgaaacc attttataac ttgttcaaca
     9121 ctatttagga ctgtcactcc atctgcttgg aattgttcat cttttgttaa gatgattgtc
     9181 tctctaccag gtaaaacacg acggttcatt ccatcaaagg tctttcgtcc cataagcaaa
     9241 gcctgcccca tggtcgtttc tttgaagtga tgtaactctt taggaagcct ccatggtaaa
     9301 ccgccattaa ctccaatcaa atggtcttca tcttcagccc aaatggcaat tatttgcttt
     9361 gtcatgactt ttttcctcac taacttatct ctagatattc taacaaattt tacctcaaat
     9421 tgcttgttaa aaaacatgag atagttagat tgctaaatca aaacgtaatt gaggtttaat
     9481 tgggtcataa tcaactaacg caaaatcatc tggtttaata tcaaaaaagt ctgttccatc
     9541 tggaacattc aatactagtt tggggttaca ctcagacggt tggcgtttta agagttcctg
     9601 agcttgttca aactggttat cataaatatg aagattattg ataaaataga aaaacttccc
     9661 aactctccaa ccaaaatgct ttgcaatcat catctgaagt gctacatact gcatagcatt
     9721 aatatgatgt gcaactaaca tgtcattaga acgttgtgtt aatgtggcat ccaaataaag
     9781 ctcaccatta acgcgtctca catcaaacat cgtttgaaaa gcacaaggta gtaggccttc
     9841 tgtttcttca aatgcttcat agtcccataa tgaaataaca ttacgacgat tccaagggtt
     9901 atcttcaagc tgttttaaga gttttgatat aatattatgt tttttaacaa ctgcaccgta
     9961 gcgctgacct atcgtgccag tatggccaac ttcccaatca ttccagtatg tcacaccata
    10021 tttatcattc aaaactacta aatcatttgt ctgatcttga taaatccaga aaatttcttt
    10081 aatagcagat tttattggaa ttggacgtag agttgtaata ggaaattcac cctttgataa
    10141 atcatactct gcaaaggcac ctgtaatata cttagaatta gccatttccc catttttata
    10201 tctaggtctg gcttgctcgc taaatacacc ttcagacata attttcgtga tattgtcttt
    10261 aaataataaa tcggcttttg tcatgagata cctccaaagt cttattttat cagattttta
    10321 aaagatattc tagtgagaaa caagactttg aaggttaaag ctttttttca tatttttagc
    10381 ttctttatgg taaaatggaa tttagcaaaa atgactttta ttcagaaagg tttaccaatg
    10441 aaaataggaa ttgataagat tggttttgca accagtcaat atgtccttga aatgactgat
    10501 ttggctattg ctcgccaagt tgaccctgaa aaattcagca agggattatt gttagattca
    10561 cttagtatta cgccagttac agaagatatc gttacacttg cagcctctgc agctaatgat
    10621 attttatctg atgaggacaa agaaactatt gatatggtga ttgtcgctac agaatcaagt
    10681 atcgatcaga gtaaggcggc ttctgtctat gtacatcaac ttttagagat tcaacctttc
    10741 gcaaggtcat tcgagatgaa agaagcatgc tatagcgcaa ctgctgctct tgattacgct
    10801 aaattacatg tagaaaaaca tccagattct aaagtattag tgatagcttc agatattgct
    10861 aaatatggca ttaaatctac tggagaatcc actcaaggtg caggcagtat cgctatgctt
    10921 attagtcaaa acccatccat tctagagcta aaagaggacc gtctagcgca aacccgtgat
    10981 attatggact tttggcgacc aaattattct gatgttcctt atgttaatgg catgttttca
    11041 acaaaacaat atctagatat gctaaaaact acttggaaag aatatcaaaa acgttttaat
    11101 actagtctaa gtgactatgc tgctttttgt ttccatattc cttttccaaa attagcttta
    11161 aagggcttta ataaaattct agataataac ttagatgaac aaaagaaagc tgaactacaa
    11221 gaaaattttg agcactctat tacctatagt aagaaaattg gaaattgcta tactggctca
    11281 ttgtatctag gacttctatc gttactagaa aatagtcaaa atttaaaagc aggtgatcaa
    11341 attgccttct tttcatacgg tagcggagct gttgctgaaa tttttaccgg tcagttagta
    11401 gacggctacc aaaataaact tcagagcgat cgtatggatc aactcaataa acgtcaaaaa
    11461 attacagtaa ctgagtatga gaaactcttc tttgaaaaaa caatcctaga cgaaaatgga
    11521 aatgctaact tcaacaccta ccggactggc actttttctc tagactctat ttgcgaacat
    11581 caacgtatct acaagaaaat taataattaa tatgacaaaa atttcctgga caggattttc
    11641 aaaaaaatcc cctgaagaac gtattcatta ccttgaagaa caagattttt tagctgattc
    11701 ttccctagaa atagtgacga atcaggactt actctcttta tcactagcca atcaaatggc
    11761 tgaaaatgtc atcggtcgaa tagccttacc attttcactt gtcccagatg ttttggttaa
    11821 cggtaaagtc tatcaagtcc catatgtcac tgaagaaccg tcagttgttg ctgctgcaag
    11881 ttttgctgct aaaattataa aacgatctgg tggttttctg acaactgtcc ataatcgtaa
    11941 aatgattgga caagtagcca tttatgatgt acaagatagt caacacacta aagaatctat
    12001 attaaatcaa aaacagcaat tattagaaat agccaacgct gctcatccat caatagtcaa
    12061 gcgtggaggc ggtgcttgcg acctaactat tgagattaaa gaagactttt tgattgttta
    12121 ccttatggtt gatacaaaag aagccatggg tgctaatatg gttaacacca tgatggaagc
    12181 attatcttcg ccattagaag acatttcaaa aggcaaaagc ttaatgtcaa ttttatctaa
    12241 ctatgctacc gaatctttag tgactgctac atgccgagtt gatttaagat ttctaagtcg
    12301 ccaaaaagag gaggccatca agttagctca aaaaatgacg atggcaagtc aattagctca
    12361 agtagaccct tatcgtgcta gcacacataa taaaggtatt tttaatggca ttgatgctat
    12421 cgtcttagct actggaaatg attggcgagc tattgaagct ggagcgcata cctatgctgt
    12481 caaagatgga caatatcgag gattatctaa atggtcttat aaggttgatg ataattttct
    12541 agaaggtact ctgacgctac cgatgcctgt tgcaacaaaa ggtggatcca tcggtattaa
    12601 ccctagtgtc caactagctc acgatctact aggtcgtcct aatgctaaag agttggcaag
    12661 tattgttctt tcgattggac tagctcaaaa ttttgcggct cttaaggcat tggttagcac
    12721 aggaattcaa gctggtcaca tgaaacttca agcaaagtcg ttggcactac ttgctggagc
    12781 taaagaagaa caaatttcag aagtcgttaa acagcttcta gactctaaac acatgaatct
    12841 agagacagca cagaaaattg tcaataaact tactaaaagt aaaaaaagaa tttgagaaaa
    12901 atcatctcaa attctttttt tacttgactt gaatttccat aagtgtataa tctagttata
    12961 aaaactagat tatatggagt tttaaaatga ataaaaatca atacccaaaa tgggaagaat
    13021 tacctgaact agatctgtat cttgaccaag tacttctcta tgtgaatcaa ttaattaatc
    13081 ctaaaacaat aactaacgat aaactactca ccgcttctat gatcaacaat tatgtcaaac
    13141 ataattatat atccaaacct attaaaaaaa aatacaatcg ccgtcaagta gcacgattga
    13201 ttgttattac agcttttaaa caagtttttg ctattcaaga aatcagtcaa acattagaaa
    13261 ttctgacagc tgacaatcat tctgaggaag catataacgg atttgctgct tgtatgaaca
    13321 aagaagaggt tcacgattta ccaccagttg tcattagtgc ctgtcagacg ttgaaccttt
    13381 atcaagaaac acaaaaatta gttttagaac taggaggaaa aaataaaaat gcttaaagaa
    13441 atgtctatca aattaagtcc tcaactttcc tttggcgaag agcttgcaaa tagtatcact
    13501 cacgctgtag gagctttgct tatgctaatc ctattaccaa tcaccgctgt ctatagtcac
    13561 aaccacttcg gattacaagc agcacttggt acctcaatat ttgtcacttc attatttttg
    13621 atgtttttat catcaagtat ttaccactcc atgacttata gcagtctcca aaaatatgtt
    13681 ctacgcatga ttgatcactc tatgatttat attgctattg caggtagtta tactcctgtc
    13741 gcattgtcac ttattggcgg ctggttagga tatctcatca tttttttaca atgggggatt
    13801 acattatttg gtatccttta taaaatcttc gctcccaaaa tcaatgataa atttagcctt
    13861 gtactttacc tcattatggg atggctggtt atctttattt tccctgctat tatcacaaaa
    13921 actggaccag ctttttgggg attactcttg gctggtggta tttgctatac tatcggtgca
    13981 ctcttttatg ctcgcaaacg tccttatgat cacatgattt ggcacctttt cattctatta
    14041 gcttctattt tacaatacat tggaattgtc tacttcatgc tataaaattt aactacattt
    14101 tattgattat ttttttgcta aaacatgata taataataat catctaaatc ttagaga
//
LOCUS       DATRTN010000042        13925 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.042, whole genome shotgun sequence.
ACCESSION   DATRTN010000042 DATRTN010000000
VERSION     DATRTN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13925
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.042"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            16..1263
                     /locus_tag="VBE22_001226"
     CDS             16..1263
                     /locus_tag="VBE22_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO7202691.1"
                     /translation="MEKNTWKKLLVSTAALSVVAGGAIAATHSNSVDAASKTTIKLWV
                     PTDSKASYKAIVKKFEKENKGVTVKMIESNDSKAQENVKKDPSKAADVFSLPHDQLGQ
                     LVESGVIQEIPEQYSKEIAKNDTKQSLTGAQYKGKTYAFPFGIESQVLYYNKTKLTAD
                     DVKSYETITSKGKFGQQLKAANSYVTGPLFLSVGDTLFGKSGEDAKGTNWGNEAGVSV
                     LKWIADQKKNDGFVNLTAENTMSKFGDGSVHAFESGPWDYDAAKKAVGEDKIGVAVYP
                     TMKIGDKEVQQKAFLGVKLYAVNQAPAGSNTKRISASYKLAAYLTNAESQKIQFEKRH
                     IVPANSSIQSSDSVQKDELAKAVIEMGSSDKYTTVMPKLSQMSTFWTESAAILSDTYS
                     GKIKSSDYLKRLKQFDKDIAKTK"
     gene            1361..2731
                     /locus_tag="VBE22_001227"
     CDS             1361..2731
                     /locus_tag="VBE22_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7202692.1"
                     /translation="MNKKLISLDGMSLKEVFQKGDLATKLSFLIMGLANLKNKQIVKG
                     LLFLISEILFLITFVYQVIPAVKGLISLGTQEQGMTTKTVDGIKIQVATQGDNSMLML
                     IFGLASLIFCCVFAYIYWSNIKSAAHLLTLKEEGREIPSFKKDIKSLTDGRFHMTLMS
                     IPLIGVLLFTILPLVYMICLAFTNYDHNHLPPKSLFDWVGFANFGNIFSGRMASTFFP
                     VLSWTLIWAVFATVTNFFFGIILALLINTKGLKFKKMWRTIFVITMAVPQFISLLIMR
                     NLLSDAGPVNALLIKWGLISSAHPLPFLSDPVWAKFSIIFVNMWVGIPVTMLVATGII
                     MNLPAEQIEAAEIDGANKFQVFQSITFPQILLIMTPTLIQQFIGNINNFNVIYLLTQG
                     GPTNSTYYQAGSTDLLVTWLYNLTVTAADYNLASVVGILIFILSAVFSLLAYTRTNSY
                     KEGAAK"
     gene            2731..3567
                     /locus_tag="VBE22_001228"
     CDS             2731..3567
                     /locus_tag="VBE22_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO7202693.1"
                     /translation="MNKKKRLNLTFVYILLIVLSIMWLFPIVWVVLTSFRGEGSAFVN
                     YFIPKTWTLDNYAKLFTQNTFPFGQWFLNTLFVATCTCILSTLITVAMAYSLSRIKFK
                     HRNGFLKLALVLNMFPGFMSMIAVYYILKALNLDQTLTALIFVYSAGAALTFYIAKGF
                     FDTIPYSLDESAMIDGATRLDIFLKITLPLSKPIIVYTALIAFMGPWMDFIFAKVILG
                     DATSKYTVAIGLFSMLQQDTINQWFMSFTAGSVIIAIPITILFMFMQKYYVEGITGGS
                     VK"
     gene            3798..5270
                     /locus_tag="VBE22_001229"
     CDS             3798..5270
                     /locus_tag="VBE22_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7202694.1"
                     /translation="MEKTKSFFGHPRGLSTLFFTEMWERFSYYGMRAILLYYMYYSVS
                     QGGLGMDKTVAASIMAVYGSLVYLSSVIGGFVSDRILGSRKTVLYGGILIMLGHIALA
                     TPFGQTALFISIALIILGTGLLKPNVSEMVGNLYGENDSRRDAGFSIFVFGINLGAFI
                     SPIVVGYLGQEVNFHLGFSLAAIGMFFGLLQYTLDGKKYLTEESLRPNDPLSPEEKSS
                     LYKKVGLILIGIVIVLILLHLMHMLTIEVIIDIFSIIAIAIPIIYFIKILSSKKISSV
                     ERSRVWAYIPLFIASILFWSIEEQGSVVLALFADEQTKLYLNFFGHHINFPSSYFQSM
                     NPLFIMLYVPFFAWLWAKWGSKQPSSPKKFAYGLFFAGASFLWMMLPGLLFGVNAKVS
                     PLWLTMSWAIVIVGEMLISPVGLSATSKLAPKAFQAQMMSIWFLSNAAAQAINAQIVK
                     LYTPDTQILYYGVVGGITVVFGFILLFYVPRIEKLMSGVK"
     gene            complement(5315..5779)
                     /locus_tag="VBE22_001230"
     CDS             complement(5315..5779)
                     /locus_tag="VBE22_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO7202695.1"
                     /translation="MSRSQKVILTNMCLIEDNKGRIVMQIRDPKRYSWSGAALPGGHI
                     EEGESLYDALVREVHEETGLTISNPKLVGMKHWYTDEGIRYLVFLYKTYDFSGELQSS
                     DEGDVVWIERSQLDKIELAYDMLNLLRVFDEDDISEMFYSKRLEDGFIRKFY"
     gene            complement(5863..6051)
                     /locus_tag="VBE22_001231"
     CDS             complement(5863..6051)
                     /locus_tag="VBE22_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202696.1"
                     /translation="MKKKNLLQESINKRLSEERYQIPKEKREKKRFDMQLILIISILI
                     GLIMSIIGIIRFFLTYSS"
     gene            complement(6062..7387)
                     /gene="gtfB"
                     /locus_tag="VBE22_001232"
     CDS             complement(6062..7387)
                     /gene="gtfB"
                     /locus_tag="VBE22_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEO7202697.1"
                     /translation="MINLFDSYTQSSWDLHFSLIKSGYINPTIALNDDGFLPDDVTSP
                     YLYYTGFAKTGAGRPLYYNELRVTDTWEIIGFSSGADIVDLGVKKGRIIYANPNHKRL
                     IKEVDWFDEQGRVILKDRFNKFGFCFAQTFYNADGQAIQTSYYNKDRQEVISENHMTG
                     DYILNDNNQFKVFKSKVEFVINYLQEAKFNLDRIFYNSLSTPFLVSFYLNRLESKDVL
                     FWQEPLVDDIPGNMRLLLNNPSPNTKIVIQSYEAYANAMRLLTDEEQKQVSFLGFMYP
                     LKETEKLHNQALILTNSDQIEALESLVTSLPNLTFNIGALTEMSSDLMNFGKYDNVVL
                     YPNITTNQIQYLSNICAFYLDINHHNEILSAVRSAFEHQQLIFAFEETSHQIRFVSPK
                     NIFPKKDIFTFISHLQPLIGNKCNIEKALKQQLEDCHVSSSTQYQSVIN"
     gene            complement(7380..8888)
                     /gene="gtfA"
                     /locus_tag="VBE22_001233"
     CDS             complement(7380..8888)
                     /gene="gtfA"
                     /locus_tag="VBE22_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEO7202698.1"
                     /translation="MTVYNINHGIGWASSGVEYAQAYRGSVLRKLGIDAKFIFTDFFS
                     ADNIISLTRNIGFQDKEIIWLYNFFSDIELAPTTFSIEDLQKQYLGKLVRKEDKGRVI
                     KFFYEDENIYLTVYLDNFNKDKVHRVEIVSNNNLIRKDYYSYTRTFSEYYYPKDGVAH
                     LYQRRFYNEDESTAFLEMVENSSSRFIINGRLLPSKVAFFDYFLESMTFTSKDIILLD
                     RGTDTAQSLLRHGKPAKLGTVVHAEHFSENAVTADTILWNNYYDYQFTNANRFDFFIT
                     STDKQTELLEQQFKQFTNHNPRIITIPVGSIDNLKMPMDNRRPYSILTASRLASEKHV
                     DWLVRAVIRIREILPEVTFDIYGSGGEEEKIRNIINAANATEYIRLMGHKNLSNVYQN
                     YELYLTASKSEGFGLTLLEAIGAGLPLIGFDVRYGNQTFIKYGENGYLIPRFDMDDEE
                     AIVEAFKEKVLQLFQQDQKALREASYAIAEGFLTNEVEGKWYNLVKELVQDD"
     gene            complement(8902..11289)
                     /gene="secA2"
                     /locus_tag="VBE22_001234"
     CDS             complement(8902..11289)
                     /gene="secA2"
                     /locus_tag="VBE22_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEO7202699.1"
                     /translation="MTAFNSLFSLDKKRLKKLQRTLNTINSLKGQMATLSNEELQAKT
                     TEFRKRLVNGETLDDICAEAFAVVREADKRVLGLFPYDVQVIGGLVLHQGNTAEMKTG
                     EGKTLTATMPLYLNALEGKGAMLLTNNSYLAIRDAEEMGKVYRFLGLSVGVGVSDNEE
                     EDRDAATKRAVYSSDIVYSTSSALGFDYLIDNLASSKSQKYMPKLHYAIVDEADAVLL
                     DMAQTPLVISGSPRVQSNLYKIADELILSFEEQVDYYFDKERQEVWIKNQGVREAERY
                     FRIPHFYKQSNRELVRHLNLSLKAHKLFERGKDYVVDDGEIKLLDATNGRVLEGTKLQ
                     GGVHQAIEQKEHLNVTPESRAMASITYQNLFRMFTKLAGMTGTGKTAEKEFIEVYDME
                     VVRIPTNSPVRRIDYPDKIYTTLPEKIHATIEFVKQVHDTGQPILLVAGSVRMSELFS
                     ELLLLSGIPHSLLNAQSAVKEAQMIAEAGQKGAVTVATNMAGRGTDIKLGKGVSELGG
                     LAVIGTERMKSQRMDLQLRGRSGRQGDIGFSQFFVSFEDDLMIESGPKWAQDYFRKNR
                     DKVNPEKPKALGQRRFQKLFQQTQEASDGKGESARSQTIEFDSSVQLQREYVYRERNA
                     LINGESGHFSPRQIIDTVISSFIAYLDGEVEKEELIFEVNRFIFDNMSYNLQGISKEM
                     SLEEIKNYLFKIADEILREKHNLLGDSFGDFERTAALKAIDEAWIEEVDYLQQLRTVA
                     TARQTAQRNPVFEYHKEAYKSYNIMKKEIREQTFRNLLLSEVSFNENGDLQIYFI"
     gene            complement(11276..12268)
                     /gene="asp3"
                     /locus_tag="VBE22_001235"
     CDS             complement(11276..12268)
                     /gene="asp3"
                     /locus_tag="VBE22_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEO7202700.1"
                     /translation="MSTISYIYWDDFSRFSYNFGTKLQFLGKSVCFENPLAPSSTNLY
                     TWSSQTNYQSKRISPNLPLLRKGTRYSLSLNAELDLVSSLFVRIEFYNRFNESVGFEL
                     LKKDSIIFIYPKEAYTYTISLINAGCSDFTFHYLKLEEVTDSVIQEKNLSTEFTIEEH
                     QDVLNLLLVEKKDSVYINKIESISQLQQKVELVSNPSLNSDSLILPELEKGLEDALKV
                     FPNIKINVIAYGTQGNFAALYYAKKFPRITAYINDCFAPFGILLKSLPHLTAKQQIFL
                     REVWDTRETSPNVKHYGLVSENSSLNLVSMILSGNEHLPYLTLLKKQDDNYDSL"
     gene            complement(12265..13824)
                     /gene="asp2"
                     /locus_tag="VBE22_001236"
     CDS             complement(12265..13824)
                     /gene="asp2"
                     /locus_tag="VBE22_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEO7202701.1"
                     /translation="MSKIKLTILQVGEENWATKENIPNNMEWLFIKPDQISDFVTTEN
                     NYLTSSKLLQKLPRKISALLLTEQTYGPELSSLSSFFEVYEVFYPKDKHATGITEEFL
                     RSKMAQRYDSSSPDQLIRQFYKGLFIGQYGEKLQVSQIQIRNDFEGVVNYQGNNYLEL
                     EGQFGENYSFLLNFAYNIPFSSDFYNELFLEHIIEGDIDIRLVISLIVDGSVDDIAKE
                     WYFEKEDLNQLISLESDISGSLAVKLFAKGKGIVKLGPLHRRNGRGGLGTFLLGGERH
                     IDAIGHEFMTYFDPVDFKPPLTVYFSGFRSAEGFEGFWMMKSMKTPFMLICDPRLQGG
                     AFYIGSKEYEQKIVDAIQEKLAFLNFSSDQLILSGLSMGTYGATYHGAKLNPHAIIIG
                     KPIFNLGTVAQRERLERPDGFATSLDIQLLNQGDLTSSSSEKLNNYFWKSIEEGDFSN
                     TTFALAYMKNDDYDATAFSDLLQYFRGKKHKILGRGWDGRHGDCSAEVGAWFTSQYRR
                     MLSNDFGRKEE"
     gene            complement(13831..>13925)
                     /gene="asp1"
                     /locus_tag="VBE22_001237"
     CDS             complement(13831..>13925)
                     /gene="asp1"
                     /locus_tag="VBE22_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase Asp1"
                     /protein_id="HEO7202702.1"
                     /translation="TNWNRSLIYSIDKINDYTGGQLVERIINSY"
BASE COUNT         4430 a         2644 c         2175 g         4676 t
ORIGIN      
        1 aggaggagaa ccaaaatgga aaaaaatact tggaaaaaat tacttgttag tactgctgct
       61 ctttcagtag ttgcaggagg agcaattgct gctactcact ctaactcagt tgatgctgct
      121 tcaaaaacaa ctatcaaact ttgggtccca acagattcaa aagcgtctta taaagcaatt
      181 gttaaaaaat tcgagaagga aaacaaaggc gttactgtaa aaatgattga gtctaatgac
      241 tccaaagctc aagaaaacgt aaaaaaagac ccaagcaagg cagccgatgt attctcactt
      301 ccacatgacc aacttggtca attagtagaa tctggtgtta tccaagaaat tccagagcaa
      361 tactcaaaag aaattgctaa aaacgacact aaacaatcac ttactggtgc acaatataaa
      421 gggaaaactt atgcattccc atttggtatt gaatctcaag ttctttatta taataaaaca
      481 aagttaactg ctgacgacgt taaatcatac gaaacaatta caagcaaagg gaaattcggt
      541 caacagctta aagcagctaa ctcatatgta acaggtcctc ttttcctttc tgtaggcgac
      601 actttatttg gtaaatctgg tgaagatgct aaaggcacta actggggtaa tgaagcaggt
      661 gtttctgtcc ttaaatggat tgcagatcaa aagaaaaatg atggttttgt caacttgaca
      721 gctgaaaata caatgtctaa atttggcgat ggttctgttc atgcttttga aagtggacca
      781 tgggattacg acgctgctaa aaaagctgtc ggtgaagata aaatcggtgt tgctgtttac
      841 ccaacaatga aaatcggtga caaagaagtt caacaaaaag cattcttggg cgttaaactt
      901 tatgccgtta accaagcacc tgctggttca aacactaaac gaatctcagc tagctacaaa
      961 ctcgctgcat atctaactaa tgctgaaagt caaaaaattc aattcgaaaa acgtcatatc
     1021 gttcctgcta actcatcaat tcaatcttct gatagcgtcc aaaaagatga acttgcaaaa
     1081 gcagttatcg aaatgggtag ctcagataaa tatacaacgg ttatgcctaa gttgagtcaa
     1141 atgtcaacat tctggacaga aagtgctgct attcttagcg atacttacag tggtaaaatc
     1201 aaatctagcg attaccttaa acgtctaaaa caattcgata aagacatcgc taaaacaaaa
     1261 tagtacgtct agagtaatga gatgaggtga gtactcttcc tttcaattga gagtgcttcc
     1321 catctcttat tttgtaaaat aacaattaaa ggagaccatt atgaataaaa aactgatctc
     1381 acttgatggt atgtccctaa aagaggtctt tcaaaaaggt gatttagcaa ctaaactatc
     1441 attcttaatt atgggacttg ctaacctcaa aaataaacaa attgtcaagg gacttttatt
     1501 tttaatcagt gaaattctgt ttttaattac ctttgtttac caagttatcc cagctgtaaa
     1561 aggtcttatt tccttaggga cccaagaaca aggtatgaca actaaaactg tggatggtat
     1621 taaaatccaa gttgcgacac aaggtgataa ctctatgtta atgctaattt ttggattagc
     1681 ttcgcttatc ttctgttgcg tatttgccta tatttactgg tcaaacatta aaagcgcagc
     1741 acatttattg accttgaaag aagaaggaag agaaattcca tccttcaaaa aagatattaa
     1801 aagcttgaca gatggacgct tccatatgac tttaatgtct attcctttaa taggggtgct
     1861 tctatttaca atccttccgt tagtttatat gatttgtttg gcatttacta actatgatca
     1921 taaccactta ccgccaaaaa gtttatttga ctgggttggt tttgctaact tcggtaatat
     1981 ctttagcggg cgtatggcca gtactttctt ccctgttcta tcttggacat taatttgggc
     2041 tgttttcgct actgtaacga atttcttctt tggtattatt cttgccttac taattaatac
     2101 aaaaggtttg aaattcaaaa agatgtggcg tactattttt gttattacca tggctgtccc
     2161 tcaatttatc tctctcttaa tcatgagaaa cctcttaagt gacgctggac cggttaacgc
     2221 cttgttaata aaatggggac ttatttcttc agcacatccc cttccattcc tttctgatcc
     2281 ggtctgggcg aaattctcaa tcatctttgt taatatgtgg gttggtattc ctgtaacaat
     2341 gcttgttgct actggtatca tcatgaattt acctgcagaa caaattgaag ctgctgaaat
     2401 tgatggggcg aataagttcc aagtctttca atcaattact ttcccacaaa ttttgttgat
     2461 tatgactcct actttaattc aacaatttat cggaaatatt aacaacttca acgttatcta
     2521 cctactcact caaggtggac caacaaactc aacatactac caagcaggta gcaccgattt
     2581 acttgtcact tggctataca atttgacagt aactgctgca gattataact tagcatctgt
     2641 tgttggtatc ttaatcttta ttctttcagc agtatttagt ttactagctt acacaagaac
     2701 aaattcttac aaggaagggg cagctaaata atgaataaga aaaaacgtct taaccttact
     2761 tttgtttata ttttattgat agtactatca attatgtggc tctttcccat tgtttgggtc
     2821 gttctcacaa gcttccgtgg tgaaggaagt gcctttgtca actactttat tcctaaaact
     2881 tggacattag acaactacgc taaactcttc acccaaaaca cattcccatt tggtcaatgg
     2941 ttcttaaata cattatttgt cgctacatgt acttgtatac tatcaacttt aatcactgta
     3001 gcaatggctt attctctcag cagaatcaaa ttcaaacacc gtaatggttt cctaaagcta
     3061 gctttagtct tgaatatgtt tcctgggttc atgtcaatga ttgcagttta ctacatccta
     3121 aaagctctaa atctagacca aacattaacc gctttaatat ttgtctattc tgcaggagca
     3181 gcattaacct tctacatcgc aaaaggtttc tttgatacta ttccatactc tttggatgag
     3241 tctgctatga ttgatggggc gacccgtttg gatattttcc ttaaaatcac cctaccattg
     3301 tcaaaaccca ttatcgttta tactgcatta atcgctttta tgggcccttg gatggacttt
     3361 atctttgcaa aagttatttt aggtgatgct acgagcaagt acacagtcgc tattggtctc
     3421 ttttcaatgt tgcaacaaga taccatcaac cagtggttca tgtcatttac agcaggatct
     3481 gttattattg caattccaat cacaatactt ttcatgttca tgcaaaaata ctatgttgaa
     3541 ggtattacag gtggatctgt aaaataatct tcagataatt taaagacacg acatttttat
     3601 ttcactgtgg tttcttttct aaaaaaggct tgggttctat tgaattcaag cctttttcta
     3661 agctagatag ttaaaaaact tggtttacca agggtgttaa cctctacaaa aaaataactt
     3721 gatagtgcta agtaatttga cagttgcttc tatttactat attattaata gtgttatcta
     3781 caaaaggagc gtttttcatg gaaaaaacaa aatcattctt tggacatcct cgtggtttat
     3841 ccactctttt ctttactgaa atgtgggaaa gattctcata ctatggtatg cgtgctattt
     3901 tgctgtacta tatgtactat agtgtttctc aaggtggcct tggtatggac aagactgtcg
     3961 ctgcatcaat catggctgtc tatggttcac tggtttatct ctcatcagta attggtggtt
     4021 ttgtcagcga ccgtattcta ggtagtcgta aaactgttct gtatggtggt attctaatca
     4081 tgctaggtca tattgctcta gctacaccat ttggtcaaac agctctattc atttcgattg
     4141 ctctaattat ccttggtact ggactattaa aaccaaatgt atcagagatg gttggtaatt
     4201 tatatggaga aaatgattct cgtcgtgatg ctggttttag tatctttgtt tttggtatta
     4261 accttggtgc ttttatttca cccattgtag tggggtacct cggacaagaa gtaaatttcc
     4321 atcttggttt ctcacttgct gctattggta tgttctttgg tctcctccaa tataccttag
     4381 atggaaaaaa atatttgact gaagagagtc tcagaccaaa cgatccttta agtcctgaag
     4441 aaaagtcctc tctatataaa aaagttgggc ttatccttat tggtattgtt attgtactta
     4501 ttctacttca cttgatgcat atgctaacaa ttgaagtaat tatcgatatt tttagtatta
     4561 ttgcaatcgc catcccaatt atttatttta tcaagatttt aagtagtaaa aagatttctt
     4621 ctgttgagcg ttctcgagtg tgggcatata tccctctctt tatcgcctca attctatttt
     4681 ggtcaattga agaacaaggt tcagttgtct tagccttatt tgcagatgaa caaacaaaac
     4741 tttaccttaa cttctttggg catcatatta atttcccatc aagttatttc caaagtatga
     4801 accctctctt cattatgctt tatgtaccat tctttgcttg gttatgggct aaatggggaa
     4861 gtaagcaacc ttcatcacct aaaaaatttg cgtatggact tttctttgct ggagcttcat
     4921 tcttatggat gatgctacca ggtttactct ttggagttaa cgctaaagta agccctcttt
     4981 ggttaacaat gagttgggct attgtcatcg ttggggaaat gctaatctca ccagttggat
     5041 tatcagcaac tagtaagctc gcacctaaag cattccaagc tcaaatgatg agcatctggt
     5101 tcttaagtaa tgctgcagca caagctatta acgctcaaat cgttaaattg tacacacctg
     5161 atactcaaat tctttattat ggtgttgttg gtggtataac agttgtattt ggatttatcc
     5221 tcttatttta tgttccacgc attgaaaaac taatgtctgg agttaaataa agacatctaa
     5281 aaagaactag gttacattcc tagttctttt ttgtttaata aaattttctt ataaaaccat
     5341 cttcaagtcg cttactataa aacatctctg agatgtcatc ttcgtcaaat actctcaaaa
     5401 ggttaagcat atcatatgct agttcaatct tatcaagttg tgaacgttca atccatacaa
     5461 catcaccttc atctgacgat tgtaattcac ctgaaaaatc atatgtttta taaaggaata
     5521 ccaaatagcg gatgccttca tctgtgtacc aatgcttcat accaactaat ttgggattac
     5581 ttattgttaa acctgtctct tcgtgaactt ctcgaacgag agcatcatac aagctttctc
     5641 cttcttcaat atggccgcct ggtaaagcag ctcctgacca agaataacgt ttaggatcac
     5701 ggatttgcat cacaatacgt cctttattat cttcaataag acacatatta gttaaaataa
     5761 ctttttgact gcgtgacatt ttttcctcta gtatataaat ttagtgatag agttacaata
     5821 ccatttattt atcaattttt ctacaaaata aaggatagta acttaactac tgtaggttaa
     5881 gaaaaatcga ataattccga taatagacat gattaaccct attaaaatag aaataatcag
     5941 aataagctgc atgtcaaatc gttttttctc tcttttttct ttaggaattt gatatcgctc
     6001 ttcagataga cgtttattaa tactctcctg aagtaaattc ttttttttca aaattttccc
     6061 cttaattaat tacagattga tactgagtag agcttgaaac atgacaatct tcaagctgct
     6121 gttttaatgc tttttctata ttacatttat tgccaattag tggctgcaag tgcgaaataa
     6181 atgtaaaaat atcttttttt ggaaaaatat ttttaggtga tacaaaacga atttgatgac
     6241 tagtctcttc gaaagcaaaa attaactgtt gatgttcaaa agcagagcga actgctgata
     6301 atatctcatt atgatgatta atatcaagat aaaaagcaca aatatttgat aaatattgta
     6361 tttgatttgt tgtgatattt gggtagagta caacattatc atatttgcca aaattcatga
     6421 gatcactaga catctcggtc aaagcaccta tgttaaaagt caaattagga agagaagtca
     6481 ctaaactttc taatgcttcg atttgatcag aattcgtcaa aataagagct tggttatgta
     6541 atttttctgt ctctttaaga ggatacataa atcctaaaaa gctaacttgt ttttgttctt
     6601 cgtcagttaa taacctcata gcattagcat aagcttcata actttgaatt acaattttag
     6661 tgtttgggga aggattgttc aataataagc gcatatttcc tggaatatca tctacgagag
     6721 gctcctgcca aaagagaaca tctttactct ctagacgatt aaggtaaaag cttactaaaa
     6781 atggtgttga cagagaatta tagaagattc tatctaagtt aaattttgct tcttgaagat
     6841 aatttatgac aaattcaact ttagatttaa aaaccttaaa ctgattatta tcatttaaaa
     6901 tataatcccc tgtcatatgg ttctctgata taacctcttg cctatcctta ttataatatg
     6961 acgtctgaat cgcttgacca tccgcgttat aaaaggtttg tgcaaaacag aacccaaact
     7021 tattaaagcg atcttttaaa atgactctac cttgctcatc aaaccagtct acctctttta
     7081 ttagacgctt atgatttggg tttgcataaa taattctgcc ctttttgaca cctaagtcaa
     7141 caatatcagc accactacta aaaccaataa tttcccatgt atcagtaact ctaagttcat
     7201 tgtagtaaag tgggcggcca gcacctgtct ttgcaaaacc agtgtaatat aaatagggtg
     7261 atgttacgtc gtctggtaaa aaaccatcat cattcaaagc tatagtaggg ttaatataac
     7321 ctgattttat caaggaaaaa tgtaaatccc aacttgattg agtgtaacta tcaaataagt
     7381 taatcatctt gtaccaactc cttaactaag ttataccatt ttccttctac ttcatttgtt
     7441 aagaatcctt cggcaatggc gtaagaagct tctcgtaaag ccttttgatc ctgttggaat
     7501 aattgtaaca ctttctcttt aaaagcttct acaatagctt cctcatcatc catatcaaat
     7561 cgaggaatta gataaccatt ttctccgtat ttgataaaag tttgattacc ataacgaaca
     7621 tcaaacccaa tcaaaggaag tcctgcgcca atagcttcaa gtaaagttaa gccaaacccc
     7681 tcacttttag aagctgtcaa atataactca taattttgat aaacattcga gagattttta
     7741 tgtcccatca atcgaatgta ttccgttgca ttggctgcat ttataatatt tctaattttt
     7801 tcttcttctc cacctgatcc atagatatca aaggtcactt caggaagaat ttctcttatc
     7861 ctaataactg cacgtactaa ccaatctaca tgtttttcac tagctaggcg tgaagctgtc
     7921 aaaatagagt acggacggcg attgtccatt ggcattttaa gattgtcaat tgagcctacc
     7981 gggatagtta taattctagg attatgattt gtaaattgtt taaattgttg ttccaaaagt
     8041 tctgtttgtt tatcagtgga ggtaataaaa aaatcaaatc tattagcgtt agtaaactga
     8101 taatcgtaat agttattcca taaaatagtg tcagcagtca cagcattctc actaaaatgt
     8161 tccgcatgaa caacagtccc tagcttagcg ggtttaccat ggcgcaaaag actctgagcg
     8221 gtatcagttc ctctatccaa aagaataata tcttttgaag tgaaagtcat tgattccaaa
     8281 aaataatcaa aaaaagctac tttggatggt aaaagtctgc cattgataat aaaacgactt
     8341 gagctatttt ctaccatctc caaaaaggct gtactttcat cttcattgta gaatctacgt
     8401 tgataaaggt gagctacacc atctttagga tagtaatact ctgagaaagt acgtgtataa
     8461 ctataataat cttttctgat aaggttgtta tttgaaacga tttctacacg atgcacttta
     8521 tccttgttaa aattatcaag gtaaactgtt aaatagatat tttcatcttc gtagaaaaac
     8581 ttgattaccc gacctttatc ttcttttcga acaagttttc caagatattg tttttgcaaa
     8641 tcttctatac taaaagttgt cggggcaagc tcaatatctg aaaaaaaatt atataaccaa
     8701 ataatttctt tatcctgaaa acctatattt cgcgtgaggc taataatatt atcagctgaa
     8761 aaaaaatctg taaaaataaa cttagcatca attccaagtt ttctcaaaac agaacctcgg
     8821 tatgcttgag cgtactcaac accgctgctt gcccaaccaa ttccatggtt tatattataa
     8881 actgtcatta tctttcatct cttatataaa ataaatttga agatctccat tctcattgaa
     8941 ggatacttca ctcaaaagta gatttctaaa agtttgttca cgaatttctt ttttcatgat
     9001 attgtaggat ttatatgctt ctttatgata ttcgaaaacc gggttacgct gtgccgtttg
     9061 acgagctgtt gccacagtac gtaattgttg tagataatct acttcttcaa tccaagcttc
     9121 atcaatcgct tttaaagcag cagtacgttc aaagtcacca aaactatcac caagcaaatt
     9181 atgcttttct cgaagaatct catcggctat tttgaataaa tagtttttaa tttcttcaag
     9241 tgacatctct ttagaaatcc cttgaagatt gtaagacata ttatcaaata taaaacgatt
     9301 tacttcaaaa atgagttctt ctttttctac ttcaccatct aaataagcaa tgaaggaaga
     9361 aatcactgta tcaataatct gtcttggact aaaatgtcca ctttctccat tgatcaaagc
     9421 atttctctca cgataaacat actctcgctg aagctgtaca ctcgaatcaa actctatcgt
     9481 ttgactacgt gcagactctc ccttaccgtc actagcttcc tgagtttgct ggaataactt
     9541 ctgaaatctt ctctgaccta aggctttagg tttttctgga ttaactttgt cacgattttt
     9601 cctaaagtaa tcttgcgccc atttaggacc tgattctatc attagatcat cttcaaatga
     9661 tacaaaaaat tgactaaaac caatatcgcc ttgtcggccc gaacgtcccc ttaattgtaa
     9721 atccattctt tgactcttca ttctctccgt tccaataact gcaagaccac ctaattctga
     9781 tactccctta cctaacttaa tatctgtacc acgaccagcc atattcgttg caacagtgac
     9841 cgcacctttt tgccctgctt ctgcaatcat ctgtgcttcc ttaaccgcac tttgagcatt
     9901 taacaggcta tgagggatac cacttagtaa taataattct gagaatagct ctgacattct
     9961 cacagaaccg gcaactagta ggattggttg tcctgtatca tgaacctgtt taacaaattc
    10021 aatcgttgca tggattttct ctggtaaggt tgtataaatc ttatcaggat agtcaattcg
    10081 tctaacagga gaattagttg gaattctaac tacttccata tcatagacct caataaactc
    10141 tttttcagca gtttttccag ttcctgtcat tccagccaat ttagtaaaca ttctaaaaag
    10201 attttgatag gtaatggaag ccattgctcg agactctggt gtgacattaa gatgctcttt
    10261 ttgctcaata gcttgatgaa cacctccttg aagtttagta ccttctaaaa cacggccatt
    10321 agtcgcatct agcaacttga tttcaccatc gtcaaccaca taatcttttc ctcgttcaaa
    10381 taatttatgt gcttttaaag ataagttcaa atgtcgcaca agttctcgat tagactgttt
    10441 ataaaaatga gggattctaa aatatctctc agcttcacga acaccctggt tttttatcca
    10501 cacttcctga cgctctttgt caaaataata atcaacttgt tcttcaaaac taagaattag
    10561 ttcatctgct attttataga gattagattg tactcgaggt gaacctgaaa taactaaagg
    10621 agtctgggcc atatctagaa gaactgcatc agcttcatct acaatagcat aatgtaactt
    10681 gggcatatat ttttgagatt tagatgaagc taggttatca attaaataat caaagcctag
    10741 tgcactactt gtactataga caatatccga actataaaca gctcgcttag tcgcagcatc
    10801 tctatcctct tcctcattat cagaaactcc cacaccgaca ctcaatccta aaaaacgata
    10861 tacttttccc atttcttccg catctcgaat agcaaggtag gaattattag tcagcaacat
    10921 tgcccccttc ccttctaaag cattgagata caaaggcata gtggcagtta atgtttttcc
    10981 ttcaccagtt ttcatctcag cagtatttcc ttggtgcaga acaagaccac cgattacctg
    11041 tacatcatac ggaaatagac ctaatacgcg cttatcagct tccctaacaa cagcaaaagc
    11101 ttcagcacaa atatcatcta gtgtttcccc attaactaaa cgttttctaa attcagttgt
    11161 cttagcttgc aactcctcat ttgatagtgt tgccatttgg ccctttaaac tattgatagt
    11221 atttaaagta cgctgaagtt tttttaaacg tttcttatct aacgaaaata aactattaaa
    11281 ggctgtcata attgtcgtcc tgctttttta aaagtgtgag gtaaggtaga tgttcattac
    11341 cagataagat cattgatact aaatttaaag aggaattttc tgaaaccaat ccatagtgct
    11401 ttacatttgg agacgtctct ctcgtatccc agacttctct aagaaatatt tgttgtttgg
    11461 cagtcaagtg tggtaaggat ttaagtaaaa ttccgaaagg agcaaaacaa tcattaatat
    11521 aggcagttat tctgggaaat tttttagcat aatataaagc agcaaaatta ccttgtgttc
    11581 catacgcaat gacattaatc ttaatgttag gaaaaacttt taatgcatct tctaacccct
    11641 tttccaactc aggtaatatc aaactatcag aatttaatga gggattgctg accaactcga
    11701 ctttctgttg caattgagaa atgctttcta ttttattaat atatacacta tcttttttct
    11761 cgaccaaaag aagatttaaa acgtcttgat gttcttcaat agtaaactct gtcgatagat
    11821 ttttctcctg aataacgcta tctgtaactt cctcaagttt caaataatga aaagtaaaat
    11881 cgctacaacc tgcattaatc aaactaatgg tataggtata agcctcttta ggatagataa
    11941 aaataatact atcttttttt aacagttcaa aaccaacaga ctcattaaag cgattgtaaa
    12001 attcaatgcg aacaaaaaga gagctaacta aatcgagttc tgcatttaat gacaagctat
    12061 aacgagttcc ctttcttagt aaaggtaagt taggagaaat tctttttgac tgataattag
    12121 tctgggagct ccatgtatac aagtttgtgc tagaaggagc taatggattt tcaaagcata
    12181 ctgattttcc taaaaattgt aattttgttc caaaattata agaaaacctt gaaaaatcat
    12241 cccaataaat gtagcttatc gtactcattc ttctttcctt ccaaaatcat tagatagcat
    12301 tcgcctatac tgacttgtaa accatgcacc aacttctgca gaacaatcac catgtcggcc
    12361 atcccaacct ctgcctaata tcttatgctt ctttcctcta aaatattgca ataaatcaga
    12421 aaaggcagtc gcatcatagt catcgttttt catataggct aaggcaaaag tcgtatttga
    12481 aaaatctcct tcttctatag atttccaaaa atagttattt aacttctcag aagaggaact
    12541 ggtcaagtca ccttgattga gtagttgaat atctaatgaa gtagcaaatc catctggtct
    12601 ctctaatctt tcacgttgag caactgttcc taaattaaat attggtttac caataataat
    12661 agcatgagga tttagcttag caccatggta agtagcacca taagtcccca ttgaaagtcc
    12721 ggataaaatt aactgatcag aactaaaatt taaaaaggcc agtttttctt gaatagcatc
    12781 aactattttt tgttcatatt ctttgcttcc tatataaaag gcaccacctt gtaatcgtgg
    12841 atcacaaatt aacatgaatg gagtcttcat actcttcatc atccagaatc cctcaaagcc
    12901 ttcagctgaa cgaaatcccg aaaaataaac agttagaggt ggtttgaaat ctactggatc
    12961 aaaataagtc ataaattcat gacctatagc atctatgtga cgttctcctc ctagtagaaa
    13021 tgtgcccaat cctcctctac cattacgacg gtgcaatgga cctaacttaa cgataccctt
    13081 tcccttagca aaaagtttaa ctgcaaggct accactaata tcggactcta aagaaataag
    13141 ttgatttagg tcttccttct caaagtacca ctctttagca atatcatcaa cagaaccatc
    13201 aacaatcaac gagataacca atcgaatatc tatatcaccc tctataatgt gttctaaaaa
    13261 taactcatta taaaaatccg atgaaaatgg aatattgtaa gcaaaattaa gcaaaaaact
    13321 atagttctca ccaaattgcc cttctaattc aagatagtta tttccctgat agtttactac
    13381 accttcaaaa tcatttctaa tttgtatttg tgaaacttga agtttctctc cgtattgtcc
    13441 tataaaaagc cctttataaa attgacgaat tagctgatct ggagagctac tatcatatcg
    13501 ttgggccatt ttcgagcgta aaaactcttc tgttatcccc gtagcatgtt tatctttagg
    13561 ataaaatact tcataaacct caaagaatga cgataaacta gataactctg gtccataagt
    13621 ttgctctgtc aacaaaagag cagatatttt tcgaggtagt ttttgcaata actttgacga
    13681 tgtaagataa ttattttctg tagttacaaa gtcagaaatt tgatctggtt taataaagag
    13741 ccattccata ttattaggga tattctcttt agttgcccag ttctcttcac caacttgaag
    13801 aattgtaagt tttatttttg acatatttat ttaataacta tttataattc tttctaccaa
    13861 ttgaccacct gtatagtcat tgattttatc aatagaataa attaaagaac ggttccaatt
    13921 agtta
//
LOCUS       DATRTN010000043        13669 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.043, whole genome shotgun sequence.
ACCESSION   DATRTN010000043 DATRTN010000000
VERSION     DATRTN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13669
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.043"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            276..1280
                     /locus_tag="VBE22_001238"
     CDS             276..1280
                     /locus_tag="VBE22_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO7202703.1"
                     /translation="MKNKGLIATLILLTILVVGELFYNKSEKHLNLSEKQVVKIGILQ
                     YVTHDALDAIEKGVEDGLAQEGYKGKKVKLTVLNAEADQSKIQAMSKQLVNHHNDILI
                     GIATPSAQGLAASTKDTPIIMGAVSDPLGAKLVTNMKKPTTNVTGLSNVVPTKQTVQL
                     IKDITPNVKRIGILYASSEDNSVSQVTEFTKYAQKAGLEVLKYSVPSTNEIKTSMSVM
                     TKKVDAVFVPQDNTIASAFRTVIVAANQANIPVYSSVDTMVEQGSIASVAQSQYGLGL
                     ETAKQAIKVLRGKPVKDVPVKVIDTGKPSLNLKAAKHLGIKIPKKIMKQAEITVKVDD
                     "
     gene            1293..2156
                     /locus_tag="VBE22_001239"
     CDS             1293..2156
                     /locus_tag="VBE22_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7202704.1"
                     /translation="MIISSVSQGLLWGILGLGIYLTFRILKFPDMTTEGSFPLGGAVC
                     VTLMNQGVNPILATILGMLSGMLAGFVTGLLYTKGKIPTILAGILVMTSCHSIMLMVM
                     KRANLGLNEIQTLKDFLPFSNDLNLLVLGLIAILLVISALIYFLYTRLGQAYIATGDN
                     PDMAKSFGIDTDKMEMLGLIVSNGLIALSGALVSQQDGYADVSKGIGVIVIGLASIII
                     GEVLYSTGLTLFERLIAIVVGSILYQFLITAVIALGFNTNYLKLFSAIVLGICLMVPV
                     LKTKILKGVRL"
     gene            2156..2917
                     /locus_tag="VBE22_001240"
     CDS             2156..2917
                     /locus_tag="VBE22_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7202705.1"
                     /translation="MVMKIIELKEATVQVSNGLAEMKTILDHVNLSIYEYDFITILGG
                     NGAGKSTLFNVIAGTLMLSGGNIYIMGQDVTILPAEKRAKYLSRVFQDPKMGTAPRMT
                     VAENLLVAKFRGEKRPLVPRKITNYTEEFQKLIARTGNGLDRHLETPTGLLSGGQRQA
                     LSLLMATLKKPNLLLLDEHTAALDPRTSVSLMGLTDEFIKQDSLTALMITHHMEDALK
                     YGNRILVMKDGKIVRDLNQAQKNEMAIADYYQLFD"
     gene            3214..4875
                     /locus_tag="VBE22_001241"
     CDS             3214..4875
                     /locus_tag="VBE22_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO7202706.1"
                     /translation="MSDIKIMALGGVRENGKNLYVVEVNDSIFVLDAGLKYPENEQLG
                     VDVVIPNLDYLIENKKRVQGIFLTHGHADAIGALPYIIAEVKAPVFGSPLTIELAKLF
                     VKNSTAVKKFNNFHVIDSETEIEFQDAVISFFKTTHSIPESMGIVIGTKEGNIVYTGD
                     FKFDQAARKYYQTDLARLAEIGRDGVLALLSDSANATSNDQVASEYEVGDEIKSVIED
                     AEGRVIVAAVASNLIRIQQVFDAAAENGRRVVLTGFDIENIVRTAIRMKRIHIADENM
                     IIKPKDMTRYEDNELLILETGRMGEPINGLQKMAIGRHRYVQIKDGDLVFIVTTPSIA
                     KEAVVARVENLIYKAGGSVKLITQNLRVSGHANGRELQLLMNLLKPKYLFPIQGEYRD
                     LSAHAGLAQEVGMSADDIYIVKRGDIMVLEKDGFFHSGSVPAGDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVVITVSKKEKKIISKARVNTRGFVYVKKSRDILRESAELVNTT
                     VEDYLSKDTFDWGELKGKVRDEVSKFLFDQTKRRPAILPVVMEVR"
     gene            4932..5720
                     /locus_tag="VBE22_001242"
     CDS             4932..5720
                     /locus_tag="VBE22_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEO7202707.1"
                     /translation="MAFFNIEYHSKVLGTERQVNVIYPDAFEISDDKIDDCDIPVLYL
                     LHGMGGNENSWQKRTNIERLLRHTNLIVVMPSTDLAWYTNTKYGLDYFDAIAIELPKV
                     LKRFFPNMSDKREKNFIAGLSMGGYGAYKIALLTNRFSHAASLSGALSFDFDLLFNNG
                     NDNINYWSGIFGDLNNTDNIERHSLRRCVESFDMKTKFYAWCGYEDFLFEANEVAIDE
                     LRQLGLTIDYFNDHGKHEWYYWNQQLEKVLEWLPIDYVKEERLS"
     gene            5810..6520
                     /locus_tag="VBE22_001243"
     CDS             5810..6520
                     /locus_tag="VBE22_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEO7202708.1"
                     /translation="MRTLFRMIFAIPKFIFRLIWNIIWGIFKTVLVIAIILFGLYYYA
                     NHSQSEFANQLSDIIQTGKTFLNFADTNQLKNSFTNLATDSVHHSENTRWDKNQATIY
                     IATRDSELVSAYKQAISNWNATGAFVLQTTDNPNADIIAKDYSDAKTQAAGVAETEKN
                     ALTNRISKVTVKLNTFYLKNKQFGYDHTRIVNTVEHELGHALGLGHNDQQHSVMQSKG
                     SHYGIQEVDIQTLKNLYA"
     gene            6758..7429
                     /gene="rpiA"
                     /locus_tag="VBE22_001244"
     CDS             6758..7429
                     /gene="rpiA"
                     /locus_tag="VBE22_001244"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEO7202709.1"
                     /translation="MDELKKLAGVTAAKYVKNGMIVGLGTGSTAYFFVEEIGRRVKEE
                     GLQVVGVTTSNRTTEQARGLGIPLKSADDIDVIDVTVDGADEVDPDFNGIKGGGGALL
                     MEKIVATPTKEYIWVVDESKLVETLGAFKLPVEVVRYGSERLFRVFKSKGYCPSFRET
                     EGDRFITDMGNYIIDLDLKKIEDPKQLANELDHTVGVVEHGLFNSMVNKVIVAGKNGL
                     DILEK"
     gene            7486..8697
                     /locus_tag="VBE22_001245"
     CDS             7486..8697
                     /locus_tag="VBE22_001245"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO7202710.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIVSAMRDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
     gene            8748..9155
                     /gene="arsC"
                     /locus_tag="VBE22_001246"
     CDS             8748..9155
                     /gene="arsC"
                     /locus_tag="VBE22_001246"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416958.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEO7202711.1"
                     /translation="MEKVRIYHNPNCGTSRNVLAIIRHCGIEPEIIYYLKTPPSRMEL
                     VELLLEMKLSARELLRTDVPAYEKFNLESSSVADEEMIDAMIQDPILINRPIVVTSKG
                     AKLCRPCEEILTILPVKMEKDFVKEDGQIIQSL"
     gene            9194..10003
                     /locus_tag="VBE22_001247"
     CDS             9194..10003
                     /locus_tag="VBE22_001247"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO7202712.1"
                     /translation="MTLLEKINETRDFLQAKGVTAPEFGLILGSGLGELAEEIENPIV
                     VDYADIPNWGQSTVVGHAGKLVYGDLSGRKVLALQGRFHFYEGNTMEVVTFPVRIMRA
                     LACHSVLVTNAAGGIGYGPGTLMLIKDHINMIGTNPLIGENLEEFGPRFPDMSDAYTA
                     TYRQKAHQIAEKQNIKLEEGVYLGVSGPTYETPAEIRAFQTMGAQAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQRIKEDFKGLVKSLVAEL"
     gene            10005..11261
                     /locus_tag="VBE22_001248"
     CDS             10005..11261
                     /locus_tag="VBE22_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEO7202713.1"
                     /translation="MVSRLYYAVKFMIAVLFMTVMAGVGAILMHYVLMFTEWLAFGDS
                     RENTLSLLNSVTPIKRVLSLTLVSFLASLSWYYLQIKPKQITSIKQQVVFKDSSVKKS
                     PYWLHIGHAFLQLIYVGAGGPIGKEGAPREFGAINAGKISDLLALKVLDKRLLIISGA
                     AAGLSAVYQVPLASVFFAFETLALGISLKNIVTLLASNFGAASIAQLVISTAPLYHIS
                     KMSLNSQLLAFMFLIVLCVTPIAISFRYLNQKVTERRIKNIKILLSLPVVSLIVSVLS
                     IVYPQILGNGNALVQEVFKGTTVSLITILVVLKMIATLSTLYAGAYGGILTPSFSIGA
                     CLGFLLASISIPLLPHISIVTSMLVGAAIFLAITMRAPLTAVGLVISFTGQSVITIVP
                     LTIAVLFATAYDYFIRKMRSLYVNPY"
     gene            11245..11955
                     /gene="deoD"
                     /locus_tag="VBE22_001249"
     CDS             11245..11955
                     /gene="deoD"
                     /locus_tag="VBE22_001249"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO7202714.1"
                     /translation="MSIHIEAKQGEIADKILLPGDPLRAKFIAENFLEDAVCFNTVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAINPDIHVRE
                     LVLAQAAATNSNIIRNDWPEFDFPQIADFKLLDKAYHIAKEMDITTHVGSVLSSDVFY
                     SNQPDRNMALGKLGVHAIEMEAAALYYLAAQHNVNALAMMTISDNLNNPEEDTSAEER
                     QTTFTDMMKVGLETLISE"
     gene            11964..12731
                     /locus_tag="VBE22_001250"
     CDS             11964..12731
                     /locus_tag="VBE22_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO7202715.1"
                     /translation="MTDFSTALKVLVDQYSYHNAFLLLQKHGPLNSDLLFLLEMMKER
                     RELNIDFLFAHQEQVVILQEKYNIKLLHNSYDLELLANYIMDLEAKVKNGLIIDFVRS
                     VSPILYRLFMILLAQEVPHLHDYIHNARDDHYDTWKFKELKESNHPVLLAFSERWHDS
                     RLTSKSLAECLQLTDLDEEVKSTIIQLRQFEKSVRNPLAHLIKPFDEQELYRTTQFSS
                     QAFLDQIIFLAKVIGVEYDTVNFHYDTVNKLIIKILE"
     gene            complement(12756..13628)
                     /locus_tag="VBE22_001251"
     CDS             complement(12756..13628)
                     /locus_tag="VBE22_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO7202716.1"
                     /translation="MNEAAKQLYITQPSLSNAVRNLETEMGIQIFIRNPKGITLTKDG
                     MEFLSYARQILEQTALLEERYKGDNTSRELFSVSSQHYAFVVNAFVALFNGTDMTQYE
                     LFLRETRTWEIIDDVKNFRSEIGVLFLNSYNRDVLTKLFDDNSLIATTLFTTTPHIFV
                     SKSNPLANRKKLSMKDLEDYPYLSYDQGLHNSFYFSEEMMSQIPHPKSIVVSDRATLF
                     NLMIGLDGYTVATGILNSKLNGDEIVAIPLDVDDVIDIVYIRHDKANLSKMGQKFIDY
                     LLEEVSFNHKNKQS"
BASE COUNT         4432 a         2161 c         2759 g         4317 t
ORIGIN      
        1 ttgcaatttt cagaatatag tatataatta tagaaaaaat catgagaaag gatgactagt
       61 aaatgactaa aacattattt accaattggc aaaactggcg tagataaagt tgtgtttatg
      121 ggtgcataga tacgactttt tggagtactt caaaaaagta tctatgcgct ggtctagtct
      181 aacaatagag agcgcaaggt aagttattta tcaggcgctt tttcttttgc gctaaactcc
      241 agaaagtcag agtattatta aagtgaggag aaaatatgaa aaataaagga ctgatagcta
      301 cgctaattct attgaccata ttagtggttg gggaattatt ttataacaaa tcagaaaaac
      361 atcttaattt atcagaaaag caagttgtta aaattggtat tttacagtat gtaacccatg
      421 atgctttgga tgctattgaa aagggagttg aagatggtct agctcaagaa ggatataaag
      481 ggaaaaaagt taaattaacc gttttaaacg ccgaagctga tcaaagtaaa attcaagcga
      541 tgagtaaaca attagttaat catcacaatg atattttgat tggaattgcg acaccatcag
      601 cacaaggttt agcagcttcg actaaggata ctcctataat catgggagct gtttctgatc
      661 ctttaggggc caagttagta acgaatatga agaaaccaac aactaatgtt acaggattat
      721 caaacgttgt tccgaccaag caaactgtgc aattaatcaa ggatataact cccaatgtta
      781 aacgtatagg aattctttat gctagtagcg aagataattc tgtgtcacaa gtaactgagt
      841 ttacaaaata tgctcaaaaa gcaggactag aagttcttaa atattctgta ccatctacaa
      901 atgaaattaa gacaagtatg tcagtcatga ccaaaaaagt agacgctgtc tttgttccac
      961 aagataatac tattgcgagt gcttttagaa cagttatcgt agcagcaaac caagctaata
     1021 tacctgtgta ttccagtgtt gatactatgg ttgaacaagg aagtatagca tcagtagcgc
     1081 agagtcaata tggcttaggg ttagaaactg caaaacaggc cattaaagtc ctaagaggga
     1141 agccggttaa agatgtacca gtcaaagtta ttgatacagg gaagccaagc ctcaatttga
     1201 aagcagccaa acacttagga attaaaattc ctaagaagat tatgaaacag gcagaaatta
     1261 ccgttaaggt tgacgattag aaagagggtt ttatgattat ttcatcagtt tcacaaggtt
     1321 tattatgggg gatattagga ttagggatat acttaacctt tagaatctta aaatttccag
     1381 acatgacaac agagggctcc ttccctctag ggggagcggt ttgtgttacc ttaatgaatc
     1441 agggggttaa tcctattttg gcgacgattc taggcatgct atcagggatg ttagcgggat
     1501 ttgttactgg tttactttat actaaaggaa agataccgac gattttagca ggaattttag
     1561 taatgacctc atgccactcc attatgctaa tggttatgaa aagagcaaat ctaggtttaa
     1621 atgagattca aacattgaaa gattttcttc cgttttcaaa tgatttaaat cttctcgtat
     1681 taggtttgat agctattctc ttggtgattt ctgccttaat ttattttctc tatacacgct
     1741 tggggcaagc ttatattgca acaggagaca accctgatat ggcaaagagc ttcggcattg
     1801 ataccgacaa aatggaaatg cttggcttaa ttgtatcaaa tggcttaatt gctttatcag
     1861 gagctttagt aagtcaacaa gatggatatg ctgatgtatc aaaaggtata ggtgttattg
     1921 ttattgggtt agctagtatt attattggag aggtacttta ctctactggt ttaactctct
     1981 ttgaacgttt aattgctata gtggtgggtt ctattcttta tcaattttta ataacggcag
     2041 taatcgcctt aggatttaat acgaattacc taaaattatt tagtgcaata gtgcttggaa
     2101 tttgtttaat ggttcctgtc ctaaaaacga agattttgaa aggagtacgc ttataatggt
     2161 gatgaaaatc atagagttaa aagaagcaac cgtacaagtc agtaatggtt tagcagaaat
     2221 gaaaacgata ttagaccatg ttaatttgag tatttatgaa tatgacttca ttacaatatt
     2281 gggtggaaat ggagctggaa aatcaacgct ttttaatgta attgcaggta ccttgatgtt
     2341 aagcggtgga aatatttaca tcatgggaca agatgttact attttaccag cagaaaaacg
     2401 agctaaatat ttatcacggg tatttcaaga tccgaaaatg ggaacagcac ctaggatgac
     2461 agttgctgaa aatttattag ttgctaaatt tcgaggtgaa aagagaccgt tagttcctag
     2521 aaagattact aactataccg aagaatttca aaaattgatt gctagaactg gtaatggact
     2581 tgatcgccat ttagagacac ctacaggttt attatcaggt ggacaaagac aagcacttag
     2641 tttattaatg gcaactttga aaaaaccaaa tctgttatta ttggacgaac acacagcagc
     2701 gcttgatcct agaacaagtg tttcgcttat gggcttgact gatgagttta tcaaacaaga
     2761 ttcgttgact gccttaatga ttacacatca tatggaagat gctctcaaat atggaaatcg
     2821 tatattagta atgaaagatg gcaagattgt cagagattta aatcaggcac aaaaaaacga
     2881 gatggctata gctgactatt atcaattatt tgattaaaaa gaagtgaaat gggagtgttt
     2941 cacttctttt tctatacact ttgcaagaaa ttattttcaa aatatgatat actaataatg
     3001 ctgtaaatcg acttgtagag acaaggaccg taagtacaaa tggaatgaaa gtaaggacac
     3061 actctttaat tcatattcct attattgatt aataaggttt ttcttcaaac ccaagaaaag
     3121 ttaccgataa atcaattact aatgataata attggacaat ttcttgtctg ctttataaaa
     3181 aagaattaac aaaatttaag gagaaaactc attatgagcg acataaaaat catggctctt
     3241 ggaggtgttc gtgaaaacgg aaaaaaccta tatgtagtag aagtcaatga ttctatcttt
     3301 gttttggatg ctggactcaa atatcctgaa aatgaacaac tgggtgttga tgttgttatt
     3361 ccaaatcttg attacttaat tgaaaataaa aaacgtgttc aggggatttt cttaacccat
     3421 ggacatgcgg atgctattgg tgcattgcct tatattatcg ccgaagtcaa agcacctgtg
     3481 tttggttcac ctctcacaat tgaattagct aagttattcg ttaaaaatag tacagcagtc
     3541 aagaaattca ataatttcca tgtgattgat agtgaaacgg aaattgaatt tcaagatgcg
     3601 gtgatttcgt tctttaaaac gactcattca attccagagt caatgggaat cgttattggg
     3661 acaaaagagg gaaatatcgt ttacacaggt gactttaaat ttgatcaagc tgcaaggaaa
     3721 tattaccaaa cagatctggc acgtttagct gaaattggtc gtgatggtgt tttagcactt
     3781 ttatcagatt ctgcaaatgc tacaagcaat gaccaagttg ctagcgaata tgaggtcggt
     3841 gatgaaatta agagcgttat tgaagacgca gaaggtagag tcatcgtagc agcagttgca
     3901 agtaatctca ttcgtattca acaagtcttt gatgcagcgg cagaaaacgg tcgtcgagta
     3961 gttttgactg gttttgatat tgaaaatatt gttcgtacgg ccattcgtat gaaacgcatt
     4021 catattgctg atgaaaatat gattatcaaa cctaaagata tgacacgtta cgaagataac
     4081 gaattattaa tccttgaaac tggacgtatg ggagagccga taaatggtct ccaaaaaatg
     4141 gcaatcggac gtcatcgtta tgttcaaatt aaagatggtg atttagtatt tatcgtaaca
     4201 acaccaagta ttgcaaaaga ggcagttgta gcgcgtgttg aaaatctgat ttataaagct
     4261 ggtggctcag ttaaattgat aacacagaat ttgcgcgtat caggtcatgc aaatggtcgc
     4321 gaattacaat tattaatgaa tctattgaaa cctaaatacc ttttcccaat tcaaggagag
     4381 tatagggatt tgtcagcaca tgcgggctta gctcaagagg taggaatgtc agcagatgat
     4441 atttatattg tgaagcgtgg tgacatcatg gttcttgaaa aagatggctt cttccatagt
     4501 ggatctgttc ctgctggaga tgttatgatt gatggaaatg ctattggtga cgttggtaat
     4561 attgtacttc gtgaccgcaa ggtattatct gaagatggaa tttttattgt tgttataact
     4621 gtcagcaaaa aagaaaagaa aatcatttct aaagctcgtg ttaatacgcg tggttttgtg
     4681 tatgtgaaaa aaagcagaga tattttgcgt gagagcgcag aattagttaa tacaacggta
     4741 gaagattact taagcaaaga tacatttgac tggggtgaac ttaaagggaa agtgcgtgat
     4801 gaggtcagca agttcttatt tgaccaaacg aaacgtcgcc cagcaatcct tcctgtggtt
     4861 atggaagttc gttgatagtg ttaagaggtt accaaatttt ggtagccttt cttgctagaa
     4921 tggaggtttt tatggcattt tttaatattg aatatcactc taaggtacta ggaacggaac
     4981 gtcaagtcaa tgttatttac ccagatgctt ttgagatatc tgatgataaa attgatgatt
     5041 gtgacattcc tgttttgtat cttctccatg gaatgggggg aaatgagaat tcttggcaaa
     5101 aacgcacaaa tatagagcgt cttttacgtc atactaatct tatcgtcgtg atgccatcaa
     5161 cagacttagc atggtatacc aacacaaagt atgggttgga ttattttgat gcaatagcta
     5221 tcgagttacc aaaagttttg aaacgtttct ttcctaatat gtctgataag agagagaaaa
     5281 atttcattgc tggattatct atggggggat atggagcata taaaatagct ctactgacta
     5341 atcgtttttc tcatgctgcc tctttgtcag gtgcactttc ttttgacttt gatttattat
     5401 tcaataatgg caacgataac attaactatt ggtcgggaat ttttggtgat ttgaataaca
     5461 ctgataacat agagcgtcat tcactaaggc gttgcgttga gtcttttgat atgaagacta
     5521 aattttatgc atggtgtggt tatgaagatt ttctttttga agctaatgaa gtcgcaattg
     5581 atgaacttcg tcaattaggt ttaactattg actatttcaa tgatcatggt aaacatgaat
     5641 ggtattactg gaatcagcaa ttagaaaaag tcttagaatg gctccctatt gattatgtca
     5701 aagaagagcg cctttcgtag tattagctaa atcgttgaaa tccaagtaaa gtcttgttat
     5761 actaaatcat taagttttgg gaaattagac acagaaggtg agataaaaaa tgagaacact
     5821 atttagaatg atatttgcta ttccaaagtt tatctttaga ttgatttgga atatcatttg
     5881 gggaatattc aagacagttc ttgttattgc gattatttta tttggcttgt attactatgc
     5941 gaatcacagt caatcagaat ttgctaatca acttagtgac attattcaga caggaaaaac
     6001 atttttaaat tttgctgaca ctaatcagct caaaaattcc tttactaatc tagctacgga
     6061 tagtgttcat cattcagaaa ataccagatg ggataaaaat caggcaacta tctatatagc
     6121 aactagagat tctgagttgg tctctgctta caagcaagca atctccaatt ggaatgcaac
     6181 aggtgctttt gtgttacaaa caacagacaa tcctaatgct gatattattg ctaaggatta
     6241 ctcagatgct aaaacacaag ctgcgggtgt agctgaaacc gagaaaaatg ctttaactaa
     6301 tcgtatttca aaggtaacag ttaaattaaa cacattttac cttaagaata aacagtttgg
     6361 ttatgaccac actaggattg ttaatacagt cgaacatgaa ttaggacacg ctttaggtct
     6421 aggacataat gatcaacaac attccgttat gcagtcaaaa ggttcgcatt atggcattca
     6481 agaagttgat attcaaacgc taaaaaactt atatgcataa aaagatgagc taagaaactc
     6541 atcttttttg tttgaacact tattttaggt gatataagtg cgttagcaat agattattaa
     6601 aaataaagaa tttgactatc gctactaact aaaatgcata aagaacaaag aaaatgtttt
     6661 caaaaatcac ttaaaaaatg gtataatgtg tcttgagata gtgttttgaa tagacttatc
     6721 aaaaatatta gataacatta acttaaaagg ggaaaatatg gacgaactaa aaaaacttgc
     6781 tggagtgacc gctgcaaaat acgttaaaaa cggtatgatt gtaggtttag ggacaggttc
     6841 tacagcatat ttttttgttg aagaaattgg acgccgtgtc aaagaagagg gattgcaagt
     6901 cgtaggcgtt acaacgtcaa accgtacgac tgagcaggca agaggattag gtattccgtt
     6961 gaaatcagca gatgatattg atgtcattga tgtcactgtg gatggagcag atgaagtaga
     7021 ccctgatttt aatggtatca agggtggcgg tggtgcgctg ctcatggaaa aaatagtagc
     7081 tactccaact aaagaatata tctgggttgt cgatgagagc aaacttgttg aaacgttagg
     7141 tgcatttaaa ttgcctgttg aggttgttag atatggttct gagcgtcttt ttcgggtttt
     7201 taaatcaaaa ggttattgtc cttctttccg tgaaacagaa ggtgaccgct ttatcactga
     7261 tatggggaac tacattatcg acctagattt gaaaaaaata gaggatccaa agcagttagc
     7321 gaatgaactt gaccatacag ttggtgttgt tgaacatggt ttatttaata gtatggttaa
     7381 taaagttata gtagcaggca aaaacggtct tgacatactt gaaaaataat tatttaaata
     7441 atcggtttta ggagctaacc gtaacctcct tagaaagaag atataatgtc acaatttgat
     7501 cgcattcact tagttgtctt agattcagtg ggtattggag cggcaccaga tgccaatgat
     7561 tttgtcaatg caggagttcc tgatggtgct tcagacacgc ttggccacat ttcaaaaaca
     7621 gttggacttg cggtgccaaa catggctaaa attggacttg gtaatattcc tcgtccccaa
     7681 gcgcttaaaa ccgttcctgc agaagaaaat ccaagtggct acgcgacgaa gttacaagaa
     7741 gtatcacttg gtaaagatac aatgacagga cattgggaga tcatgggctt aaatattaca
     7801 gagccatttg atactttctg gaatggtttt ccagaagata ttatcacaaa gattgaggat
     7861 ttttcaggac gtaaagttat tcgtgaagcg aataaacctt attcagggac agctgttatt
     7921 gatgacttcg gaccacgcca aatggaaact ggtgagctca tcatctatac ctcagctgac
     7981 ccagtcctac aaattgcagc tcatgaggat atcattcctc tagaagaact ctaccgtatt
     8041 tgtgaatatg ctcgtagtat cacaatggag cgtccagctc tccttggtcg tattatcgct
     8101 cgtccatatg taggtgaacc aggcaacttt acgcgtacag ccaaccgtca cgactatgca
     8161 gtatcaccat ttgaagatac cgtattaaat aaactagatc aagctggcat cgatacttat
     8221 gctgttggta aaatcaatga tattttcaat ggttcaggca ttaatcatga tatgggacat
     8281 aacaaatcaa atagtcatgg tattgatact ttgataaaaa caatggggtt atcagagttt
     8341 gaaaaaggct tctcctttac taatttggtt gactttgatg ctctctatgg tcaccgtcgt
     8401 gatcctcatg gttatcgtga ttgtcttcat gagtttgatg agcgtcttcc agagattgtt
     8461 tcagcgatgc gtgacaaaga cttacttttg atcactgccg atcatggtaa tgacccaact
     8521 tatgcaggta cagaccatac aagagaatac attcctctct tagcctatag cccatctttc
     8581 acaggaaacg gccttatccc agtagggcat ttcgcagata tttcagcaac agttgcggat
     8641 aactttggtg ttgatactgc gatgattgga gagagcttct tgcaggactt ggtataaatt
     8701 tttatttcct taagacactt gtagcattaa aaattgagag gaagatgatg gaaaaagtta
     8761 ggatttacca taacccaaat tgtgggacat cgaggaacgt acttgccatt attcgtcatt
     8821 gtggtattga accagaaatt atttattatc tgaaaacacc acctagcagg atggaactgg
     8881 ttgaactgtt gttagaaatg aaactttctg cgagggaact tctaagaaca gatgttcctg
     8941 cttatgagaa attcaatttg gaatcttcta gcgttgcaga tgaagaaatg attgatgcga
     9001 tgatacaaga tcctattctt atcaatcgtc ccatagttgt tacgagtaaa ggtgcaaaac
     9061 tctgccgacc ctgtgaagaa attttaacca ttttacctgt aaagatggaa aaagattttg
     9121 ttaaagaaga tggacagatt attcaaagtt tatagcatat tgtatagtga ataatggttt
     9181 aggaggtaag taaatgacat tattagaaaa aattaatgag actagagact ttttgcaagc
     9241 aaaaggcgtc acagcaccag aatttggcct tattttaggc tctggtttag gagaattggc
     9301 tgaagaaatc gaaaatccta ttgttgtgga ttatgcagac atcccaaatt ggggacagtc
     9361 aacagtagtt ggtcatgctg gaaaattagt gtatggggat ttatcaggcc gtaaggtatt
     9421 agcgcttcaa ggtcgttttc acttttatga aggaaataca atggaagtcg ttactttccc
     9481 agtacgtatc atgagagcat tggcttgcca cagtgtgctt gtgactaatg cagcgggtgg
     9541 gattggatac ggaccaggaa ctttaatgct gatcaaagac cacatcaata tgattgggac
     9601 taaccctctc ataggtgaga accttgaaga atttggacca cgtttcccag acatgtcgga
     9661 tgcttataca gcaacatatc gacaaaaagc tcaccaaatt gctgaaaaac agaatatcaa
     9721 actcgaagaa ggtgtgtact tgggtgtatc aggacccact tatgaaacac ctgcagaaat
     9781 tcgtgcattc caaacaatgg gcgcacaagc ggtaggtatg tccacggttc cagaggtgat
     9841 cgttgcagct cactcagggc ttaaagtgtt aggaatttca gcaattacta actttgccgc
     9901 tggcttccaa tcagagctca atcatgagga ggtcgttgaa gttactcagc gtattaaaga
     9961 agatttcaag ggattagtta aatcattagt tgctgaactt taaaatggtt tctcgattat
    10021 actatgctgt caagtttatg atagccgttc tatttatgac agttatggca ggagttgggg
    10081 ctatcttaat gcactatgta ttaatgttta cagagtggtt agcatttggt gatagtagag
    10141 aaaatactct gtctttgtta aatagtgtca caccaattaa aagagtgtta tcacttactt
    10201 tagtctcctt tttagcatcc cttagttggt attacttaca aatcaaacca aagcaaataa
    10261 ctagtattaa acaacaggtt gtttttaagg attcttctgt aaaaaagtca ccttactggc
    10321 tacatatagg tcacgcattt ttgcaattga tttatgtagg agctggtggt cctattggaa
    10381 aagaaggagc accacgagag tttggagcca taaatgctgg gaaaatatcg gatttattag
    10441 cacttaaagt gctggataaa cgtttattaa tcattagtgg tgcagctgca ggtttgtcag
    10501 cagtttatca agtaccccta gctagtgtat tctttgcttt tgaaacttta gctttaggaa
    10561 tctcactgaa aaatattgtg acattgctag cctctaattt tggtgcagct agtattgctc
    10621 aattagttat ttcgacagcc cctctttatc atatttcaaa gatgagtctg aattcacagt
    10681 tattagcttt tatgttcttg atcgttttat gtgtaactcc gattgcgatt agctttagat
    10741 atttaaatca aaaagtgaca gagagacgta ttaaaaatat aaaaatattg ttaagtttgc
    10801 cagtagtgtc attgatagta tctgttttat caatagtcta tccccagatt ttaggaaatg
    10861 gtaatgccct agttcaagag gtctttaaag ggaccacagt tagcttaatc actattttag
    10921 ttgttctcaa gatgatagca acattatcca ctctttacgc tggtgcttat ggcggtatac
    10981 tgacaccatc tttttccatt ggtgcttgtt taggtttttt gttagctagt atttctattc
    11041 ctttacttcc ccacatatcg atagtaacca gtatgttggt tggtgcagct atttttttag
    11101 ctattacaat gagagcgcca ctgacagctg tcggtttagt catatctttt actggtcaat
    11161 ccgttataac tattgttccc ttaactatag cagtactttt tgctactgcc tatgattatt
    11221 ttataagaaa aatgaggagt ttatatgtca atccatattg aagcaaaaca aggtgaaatt
    11281 gcagataaaa ttttacttcc aggagatcca ctccgtgcga aatttattgc cgagaatttt
    11341 ttagaagatg cggtgtgttt taatacagtt cgcaatatgt ttggttatac aggtacctat
    11401 aaaggacatc gtgtttctgt tatgggaact ggtatgggta tgccttcaat ttcaatttat
    11461 gctcgcgaac taattgttga ttatggggtt aaaacattga ttcgcgtagg aacagcaggt
    11521 gctattaatc ctgatatcca tgttcgtgaa cttgttttag cacaagcagc agcaacaaat
    11581 tctaatatta tccgtaatga ttggcctgaa tttgattttc cgcaaattgc tgattttaaa
    11641 cttttggata aagcttatca catcgcaaag gaaatggata ttacaactca tgtaggcagt
    11701 gtattatctt cagacgtgtt ttattcaaac caaccagatc gtaatatggc cttgggtaaa
    11761 ctaggtgttc atgctattga gatggaagca gcagctctct actatcttgc agctcaacac
    11821 aatgttaatg ctcttgctat gatgacgatt tcagataact tgaataatcc agaagaagat
    11881 acaagtgcag aagagcgtca aacaacattt actgatatga tgaaagttgg gctcgaaaca
    11941 cttatttctg aatgaggata gaaatgacag atttttcaac tgctcttaaa gtattagtgg
    12001 atcagtattc ttatcataat gctttcttat tattacaaaa acatggtcct ttaaactcag
    12061 atttattatt tttattggaa atgatgaaag agcgtcgaga gttaaacatt gattttcttt
    12121 ttgctcatca agaacaagtt gttatattac aagaaaaata caatataaag cttttgcata
    12181 actcgtatga tttagagttg ctggcaaatt acatcatgga tttagaagca aaagtaaaaa
    12241 atggattaat catagatttt gtccgtagtg ttagtcctat tttatatcgt ctatttatga
    12301 ttttacttgc acaagaagta cctcacttgc atgattacat ccataatgca agagatgatc
    12361 actacgatac ttggaagttt aaagaattaa aggagtcaaa ccatccagtc cttttggcat
    12421 tctctgaaag gtggcacgat agtcgcttga cttctaaaag ccttgcagaa tgtttacaat
    12481 taaccgacct tgatgaagaa gtgaaatcga ccatcattca attaagacag ttcgaaaaat
    12541 cagtcagaaa tcctttggct cacctgatta aaccttttga tgagcaagaa ctatatcgca
    12601 caactcaatt ttcttctcaa gcatttttag accagattat cttcttggca aaggtaattg
    12661 gtgttgagta tgatactgtt aattttcact acgatacggt taacaagctt attataaaga
    12721 tacttgagta atttaaaaac agttctgaga caatctcagg actgtttatt tttatgatta
    12781 aacgaaactt cctctaataa atagtcaata aatttttgtc ccatttttga aaggtttgct
    12841 ttgtcgtgtc ggatatagac aatatcaata acatcatcca cgtcaagagg gattgcgaca
    12901 atttcgtcac cgttcaattt tgagttgagg ataccagttg ccactgtata accatctaat
    12961 ccaatcatta aattaaacag agttgctcta tcactgacaa caatggattt tgggtgagga
    13021 atttgagaca tcatttcctc agaaaagtag aaagaattat gcaggccttg atcataactc
    13081 agataaggat aatcttctaa atctttcata cttaattttt ttctattagc taagggattg
    13141 gatttgctca cgaaaatatg aggtgtagta gtaaaaagag ttgtggcaat taaggaatta
    13201 tcgtcgaata atttggttaa tacatctcta ttatagctat taagaaacag aacaccaatc
    13261 tctgaacgga aatttttaac atcatcaata atttcccaag tcctagtttc acgtaaaaaa
    13321 agttcgtatt gggtcatgtc tgtgccatta aagagagcta caaaagcgtt aaccacaaat
    13381 gcatagtgtt gtgaagaaac gctgaacagc tcacgacttg tattatcacc cttataacgc
    13441 tcttcaagaa gtgctgtttg ttctagaatt tgtcgcgcat atgacagaaa ttccatacca
    13501 tcttttgtta acgttattcc ttttggattt cgaataaaaa tttggatccc catctcagtc
    13561 tcaaggttcc taacagcgtt tgaaagtgaa ggttgggtaa tgtagagttg tttagcggct
    13621 tcattcatgc taccagtttc tacaatctta ataacatatt gtaattgtt
//
LOCUS       DATRTN010000044        13178 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.044, whole genome shotgun sequence.
ACCESSION   DATRTN010000044 DATRTN010000000
VERSION     DATRTN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13178
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.044"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..783
                     /locus_tag="VBE22_001252"
     CDS             <1..783
                     /locus_tag="VBE22_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO7202717.1"
                     /translation="IFYDEPTFVVIANKDDLPAIQLIEESQKPGIYILLGDNKRYVGQ
                     ASNSIFSRLQQHNTNKPWWNKLIFFGREDGHLSKAQLDLLERLIIEKMNTANIDLDNS
                     TQGNKSYIDKLSKFSALSLWNKVEKLLNDVANIDLFDSEHIQTEDNSDNTTLNSHISI
                     IFGESLYTGKSYRDVFTQLVSNLVLSAELDKLIPLMSPNEPNTVQILGNREHISARGT
                     KLTKPIELTKYHMYVNFSKIGLYNQIKKLAELTGKKVIFERW"
     gene            789..1379
                     /locus_tag="VBE22_001253"
     CDS             789..1379
                     /locus_tag="VBE22_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7202718.1"
                     /translation="MVQQIVLPIKDSNVLKMVQDTLLDSFRAGRRNYTIFQVGKATLL
                     RVSDVMKLKKTDVFNLDGTVKQTAFIHDQKTGKKNTLYLKPVQQDLMLYHAWLIQQNM
                     NSEWLFPSTSRPDRPITEKQFYKIMARVGDLLGINYLGTHTMRKTGAYRVYTQSNYNI
                     GLVMHLLNHSSEAMTLAYLGLDQASRENMLDQIDFG"
     gene            complement(1759..3663)
                     /locus_tag="VBE22_001254"
     CDS             complement(1759..3663)
                     /locus_tag="VBE22_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEO7202719.1"
                     /translation="MKSLITALKEKRALRLYFIGIIIFIIGLLLPISSTVRVLISIIA
                     TILSGYHVMLEGIDDTIKETVKKKRFTPNTHILMTLAAIGAIFLGEAVEAALLIFIFA
                     GAHFLEEYVEGKSKREITKLIELNPTEARLINNEGNIEVVSIDKLKIGDRLQVLNGAQ
                     VPTDGIILNGSTSIDESTINGESIPREKNVGDIVFGSTINGTGTFTMRVSKDSKDTVF
                     SKILTMVEESQNNLSPTADFIKRFEPHYVNGVLVIFLALIIFSPMFLGWTLTETISRS
                     LVFLVSASPCALAVAAIPATLAGISSLAKQGILFKGGNFLSNLINLKAVAFDKTGTLT
                     EGKPKVVDFYFNPELDGESFRNTLVAMEKQSNHPLANAIVNKFEIQKVDYDIKVENEL
                     GTGLSATYQGESYRIAKPSAFKIVSQEWIEKQENFENEGKTVVFIAVNDKVEGLIAIQ
                     DTPQKTAISAIQYLNKNQIKTIMITGDAVLTGKAIGKQLGVRDVQANVMPDEKAKIIN
                     ENKEKYGPTAMIGDGVNDAPALAVADVGIAMGDGTDVAIETADVVLMNNDLGNFVSAH
                     KTSKRLKNIVTQNIIFALLVVLFLVYISLWKNTSVVLSITLHEGSTLVVLLNSLRLLL
                     PNKKDIEGEL"
     gene            3754..4533
                     /locus_tag="VBE22_001255"
     CDS             3754..4533
                     /locus_tag="VBE22_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7202720.1"
                     /translation="MENKNYLTIGKLSSYSGIHIKALRYYESIGILHPALIDANNGYR
                     YYSHAHIPYVKIIKLCADYGIPLGTFNDFIKSETEINLIEIIRKASNILELKEKQLKE
                     DKQFLEYIQNEISLSQKLDSSINVDLEQTHEDFILIPFDDVFFSNKYYDTINKTISML
                     KDLEIEFFNRIGCYYSLQYGNYDKFLALKVRGHSSKLKNIKILHIDHVCVQAQHIEPD
                     KITDELIRLKKLKQNSILILETYENPYNFSTPHLELRILLN"
     gene            complement(4586..4966)
                     /locus_tag="VBE22_001256"
     CDS             complement(4586..4966)
                     /locus_tag="VBE22_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7202721.1"
                     /translation="MQQNHPYAGGGCTDFVWQYFAAKGIYIANIVNGNGGYWGTNGVS
                     QGVLRRTNLAPGVIASGFTDHFTGYGTSTTARTSPYGHVAVVTGVNPDGTFNVQEAGY
                     GGTFPWGNVRTNISPENVVFLLPN"
     gene            5027..5707
                     /locus_tag="VBE22_001257"
     CDS             5027..5707
                     /locus_tag="VBE22_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEO7202722.1"
                     /translation="MNHFKGKQFQKDVIIISVGYYLRYNLSYRDVQEMLYDRGINVSH
                     TKIYRWVQEYGKLIYQIWKKKNRQSFYSWKMDETYIKIKGKWHYLYRAIDSEGMTLDI
                     WLRRKRNTQSAYAFFKRLYKQFGEPKVIVTDKAPSIASAFRKLQKQGLYSETEHRTVK
                     YLNNLIEQDHRPVKRRNKLYQSLRTASTTIKGIEALRGIYKKNRRNGTLFGFSTSTEI
                     KVLMGIPA"
     gene            6249..6614
                     /gene="mobC"
                     /locus_tag="VBE22_001258"
     CDS             6249..6614
                     /gene="mobC"
                     /locus_tag="VBE22_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017369566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEO7202723.1"
                     /translation="MDQKEVSQNQTKYIQFRLSEEQYNKLKISGETYGLSPNLYAKKL
                     AQKSHLKKPYLEHDQAKALLLELSKQGTNLNQIAKKLNQFDRMDNQDKELIEALRYTY
                     GVLAQAQKGYQELWQQLQK"
     gene            6593..7825
                     /locus_tag="VBE22_001259"
     CDS             6593..7825
                     /locus_tag="VBE22_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEO7202724.1"
                     /translation="MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPV
                     ELTNCRAVAVGGTNGIDPFIAKEQFEVVRQLHNQTKESNQVLRITQSFALDELNPKVQ
                     KDWQKANDLGVELAENLYPNHQSAVYTHLDGKNHVLHNHIIVNKVNLKTGKKLREQKG
                     ESVQRAREMNDRLASRENWHILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQD
                     VSVSSYETFKARLSDNGVILSERGQTFSYAFLDANNKQRRAREARLGSDFGRETILHE
                     LENRARQNEFRAVEQGEPAITPLERDTQQRESEIVSLEQAIEPRKSEALKRESTINRF
                     IDTIKQFAGRVPELTQRVTRKLKQTKEKILDDFECRFSKDMKNDEQEQQKSLEKQANR
                     DVQSEKKPTKDHDRGMSR"
     gene            7822..8445
                     /locus_tag="VBE22_001260"
     CDS             7822..8445
                     /locus_tag="VBE22_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HEO7202725.1"
                     /translation="MNKDEQLIVQVLNAYKNGKIDFSHVPELDRLVRQEVNKEFRDYQ
                     EKIEAVANQKMESAIQEQLQRLEAENLKATILKDIQVEKQALLALKKELNEQKEQIKA
                     DRKREIVERYGILIANIVCLFCFLVVGILIGRWIYKGIWDGWGLHILYDTVIEIQPKH
                     RYGAVVLGLGGFGLIGAGIYGSFRLMYTASTWFDQRPKIFKRIFPKK"
     gene            8462..9064
                     /locus_tag="VBE22_001261"
     CDS             8462..9064
                     /locus_tag="VBE22_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEO7202726.1"
                     /translation="MVLENKLGLTNSAELAKQEELLTKKRAKELFESGKIKDLEIGTF
                     QGLSDIHQFLFQDIYDFAGKIREVNIAKGNFQFAPRIFLAQTLEYIDKLPQETFDEII
                     DKYSDMNVAHPFREGNGRATRIWLDLILKNKLHKIVDWNQIDKDEYLNAMIRSTVSTN
                     ELKYLIQKALTDDLGKEQFFKGIDASYYYEGYYEIKTEDL"
     gene            9471..10664
                     /locus_tag="VBE22_001262"
     CDS             9471..10664
                     /locus_tag="VBE22_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEO7202727.1"
                     /translation="MSSIPEKQENQKQVLTLNELSKRKVVEHNSLITSIAKMDKTPLK
                     MFELAVSCINTEEPPKDNIVYLSKRDLFAFFKVSDNDKHSRFKEAIEKMQKQAFFQIK
                     EEAGKGFKFKSIVPIPYVEWTDNHDEVKIEFHREIMPYLINLKKNFTQHALSDLAELN
                     SKYSIILYRWLSMNYNQYEHYSAKGGRRAEQVESYRNPSISIKELRIMTDTVNDYERF
                     QSLETWILKKPLEEINAHTSFNVTYDKIKKGRSIDSIVFHIEKKRCADDNSYKLNDKM
                     YQAEQAKKEETEDRLAIEAMKNKYTKLLLDNMLLNSYEMTDTAIMAGLQQHVYPLYDE
                     LKDLHGMKAVQDHLSYVASKQEAYSKRNIAKYLKKAIEQYLPTVRGSEIENMADLLKK
                     IEGKA"
     gene            10661..11203
                     /locus_tag="VBE22_001263"
     CDS             10661..11203
                     /locus_tag="VBE22_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202728.1"
                     /translation="MSEDLKTIKELAEELGFIKQTIQYHVKFLPTKNRLKNSSGAIVL
                     NLEEQRFIKNRLTKNRPNNDQKSTKEPTKNRPKKDKEISINNVLLEQLKTKDEQIKSL
                     VEAQKQTQNLLDQQQRLALQDKKLLDEYKSEINDLKALKMPTQDTKVEQVSSKQPQEV
                     ESVAEEPKKAPRKWWGLWRK"
     gene            11191..11802
                     /locus_tag="VBE22_001264"
     CDS             11191..11802
                     /locus_tag="VBE22_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO7202729.1"
                     /translation="MAKIDDSVKKKVPELRFPGFTDDWEERKLKDIAQFNPKTVLPDE
                     FEYVDLESVVGTEMISHRTESKDQAPSRAQRLAQKGDVFYQTVRPYQMNNYLYDLPYD
                     NYVFSTGYAQMRPNIDSYFLLNSVQNKQFVQHVLDRSTGTSYPAINSSDLSNIEIHVP
                     SKLSEQQKIGAFFQSIDDTITLHQRKLDLLKEQKKGFLQKMFV"
     gene            12087..13013
                     /locus_tag="VBE22_001265"
     CDS             12087..13013
                     /locus_tag="VBE22_001265"
                     /inference="COORDINATES: protein motif:HMM:NF025713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEO7202730.1"
                     /translation="MEDESYSILNQRAIIDLLIQGDIFPYMTGSEIQDIGIKFGVSYE
                     GGSRWQILQSILFSLHENGKTSDFFYYYLFKMNITSAVREVIDGNINAKPKYTKVEEI
                     VEESEQNNKAIASVLRTDLINKINLELSVSDKKLIVLNEKIAIIDSKATPKVLVDPAE
                     IMNIGYVKKILNNAKEDLLNNDLDSVVTKSRTIIETVFIVILRKHQIDFKENGNIGHY
                     RSLVNKTLGMKPDNRWDENVSSLISGLNKIVDSITTMRNINSDSHGSSNRVKINEAEA
                     ELILNSAVNISVYYLRVDGRKEKNSVNITKTS"
BASE COUNT         4785 a         2134 c         2298 g         3961 t
ORIGIN      
        1 attttttatg atgaacctac ttttgtagtt attgccaata aagacgattt accagcaatt
       61 caattaattg aggaatctca aaaaccaggt atatatatat tactaggaga taataaacgt
      121 tatgtaggtc aagcctctaa ttctattttt tctaggttac aacaacacaa tacaaataaa
      181 ccatggtgga acaaattaat attttttgga cgtgaagatg ggcatctatc taaagcacaa
      241 ctagatcttt tagaacgttt aatcattgaa aaaatgaata ctgctaatat tgatttagat
      301 aatagcactc aaggaaataa aagctatatt gataaattaa gcaaattttc agctctgtca
      361 ttatggaata aggttgagaa actattgaat gatgttgcca atatcgattt atttgattca
      421 gaacacattc aaactgaaga taatagtgac aacacaactc taaatagtca tatatcaatc
      481 atatttggag aatcattata tactgggaaa tcatatagag atgtatttac gcaattagtt
      541 tctaatttag tattatccgc agagttggat aaattgattc ctttaatgag tcctaatgaa
      601 cccaatacag tacaaatact tggcaataga gaacatatat cagctagagg gacgaagttg
      661 actaaaccaa tagaactaac taaataccat atgtatgtta atttttcaaa aataggctta
      721 tacaatcaaa tcaaaaaatt agctgaactg actggaaaaa aagttatatt tgaaaggtgg
      781 taattcatat ggttcaacaa atcgttcttc ccatcaaaga ttcgaacgtc ttaaaaatgg
      841 tgcaggatac actacttgat agttttcgtg ctggtcgtcg caactataca attttccaag
      901 ttgggaaagc gaccttgcta cgtgtgagtg acgtcatgaa attaaaaaaa acagatgtct
      961 ttaatcttga tggaacagtc aaacaaactg catttattca tgatcaaaaa acaggtaaga
     1021 aaaatacgtt atatttgaaa cctgtccaac aagatttaat gctttatcat gcatggctca
     1081 tccaacaaaa tatgaattca gagtggttat ttccttcaac ttctcgtcct gatcgtccta
     1141 ttaccgagaa acagttttat aagattatgg cacgagttgg tgatctttta ggaattaact
     1201 atctaggaac gcataccatg cgtaaaacag gcgcttatcg ggtctatacg cagtcaaact
     1261 ataatattgg tttagtgatg catttactaa accattcaag cgaagcaatg actttagctt
     1321 atctcggttt agatcaagcc agtcgagaga atatgttaga ccaaatagat tttggataat
     1381 attttttatt taaaaagagg gatgatcatt atgttaaaaa attacattag ttggtttaaa
     1441 aaaaatataa ataaacctgt tttcaggatg atatttattg ttttagtagt cacatttacc
     1501 actctaatta taaatatcat ccaaggagat cctatacttc aaaatatttg ttgtgttatt
     1561 taactttcat caatcgtaat ccgtttaatg ttactaaaag cgtttcatat tctcaaaaga
     1621 tgagaaaaat ttatattatc ccaaaaattt cgtaatacta tctgcaacat tttgaatttg
     1681 ttcaggaata ttttcggtta tattccccaa aatttcttgt gcttgttcag ttaagttttg
     1741 aaatatattt ttaaacattt atagctctcc ttctatatct tttttatttg gcaataataa
     1801 tctcaatgaa tttagcagaa caaccagcgt acttccctca tgcaatgtta tgcttagtac
     1861 caccgaggta tttttccata gactaatata cactaaaaat agaacaacta ataaagcaaa
     1921 tattatattt tgagttacaa tattttttaa tcttttagaa gttttatgtg cactcacaaa
     1981 attaccaagg tcattattca ttaatactac atctgcagtt tctatagcta catccgtgcc
     2041 atctcccatc gcaataccta catctgctac agcaagtgct ggtgcatcat tcacaccatc
     2101 tccgatcatc gcggttggac catatttttc tttattttca tttattattt ttgccttttc
     2161 atcaggcatt acatttgcct gcacatctct tacgcctaac tgtttcccaa ttgcctttcc
     2221 cgttaatact gcatcaccag ttatcataat agtctttatt tgattcttat ttaaatattg
     2281 aattgccgaa attgcagtct tttgaggagt atcttgaatt gctattaagc cttcgacttt
     2341 atcgttaaca gcaataaata ccaccgtttt tccttcattt tcaaagtttt cctgtttttc
     2401 aatccattct tgacttacga ttttaaaagc acttggttta gcaattcgat agctttctcc
     2461 ttggtaggtt gcgctcaacc ctgtcccaag ttcattttct actttgatat cgtagtcaac
     2521 tttttgaatt tcaaatttat taactatagc atttgctaat gggtgattac tttgcttttc
     2581 cattgctact aatgtatttc taaaggattc accatcaagt tcgggattaa aatagaaatc
     2641 tactactttt ggttttcctt ctgtcaatgt tcctgtttta tcaaaagcga cagcttttaa
     2701 attaattaaa tttgaaagaa aattaccacc tttaaatagt attccttgtt ttgctaaaga
     2761 tgatattcca gctaatgttg caggaattgc agcaacagct aatgcacatg gtgaagcaga
     2821 gactagaaag actaaacttc ttgaaattgt ttccgttaat gtccagccta aaaacattgg
     2881 agaaaatata attaaagcta aaaatattac taatactccg ttgacatagt gtggttcaaa
     2941 acgttttatg aaatcggcag ttggtgaaag gttattttgt gactcttcaa ccatagttaa
     3001 tattttagaa aatactgtat ccttgctatc cttggatact ctcatagtga atgtacctgt
     3061 accattaatg gttgaaccaa aaacaatatc accaacattt ttttctcgag gaatactttc
     3121 accatttatg gttgactcat caatgcttgt tgaaccatta agtatgatac catcagttgg
     3181 aacttgcgct ccattcaaaa cttgaagtct atcacctatt tttaatttat caatcgaaac
     3241 tacttctata tttccctcat tattaattaa acgagcttcc gtaggattta gttctattag
     3301 tttagtaatt tcccgtttac tttttccctc aacatattct tcaagaaagt gtgcccctgc
     3361 gaatataaag attagtagag cagcttcaac ggcttctcct aagaaaattg cacctattgc
     3421 ggcaagtgtc attaaaatat gcgtattagg agtaaatctt ttctttttga ctgtctcttt
     3481 tatggtatca tctatccctt caagcattac atgatatccc gataaaatag ttgctattat
     3541 gctaattaaa actctgactg tacttgatat tgggagtaat aatccgatga taaaaattat
     3601 tattccgata aaatataagc gtaatgctct cttttctttt aaagctgtaa ttaagctttt
     3661 catattagat acctcttcta aatgatataa atatttttat atgttatact gatattataa
     3721 actatacctt tacggtaagg tcaaggagaa agaatggaaa ataaaaatta tttgactata
     3781 ggaaaactta gcagctattc tggtatacat atcaaggctt taagatatta tgagtctatc
     3841 ggcattttac atccagcatt aattgatgct aataatggtt accgctatta ctctcatgca
     3901 catattccat atgtgaaaat tataaaatta tgtgctgatt atggaattcc tctagggact
     3961 tttaacgact ttattaagtc cgaaactgag atcaatttga tcgaaatcat tagaaaagct
     4021 agtaacatat tagaattaaa agaaaagcaa ttaaaagagg ataagcaatt tttagaatat
     4081 atacaaaatg aaataagctt atctcaaaaa ctagactcat caattaatgt tgatttagag
     4141 caaactcatg aggattttat cttgatacct ttcgatgatg tatttttttc aaataagtac
     4201 tatgatacaa ttaataaaac tatctctatg ttgaaggatt tagaaataga attttttaac
     4261 cgaattgggt gttattattc actccagtat ggtaactacg ataaatttct tgccttaaaa
     4321 gttcggggcc actcatcaaa gttaaaaaac ataaaaattt tgcatattga tcatgtctgt
     4381 gttcaagcac aacatattga acctgataag ataacggatg aattaattag acttaaaaaa
     4441 cttaagcaaa attctatatt aattctggaa acttacgaaa atccgtacaa cttttcaacg
     4501 ccacatctgg aattaaggat attattaaat taattggcag ttatctaaaa atacaatact
     4561 agatatgtat ctagtattgt attttttaat ttgggagtag gaatactaca ttttctgggc
     4621 taatatttgt tctaacattt ccccaaggaa atgtcccacc atatccagct tcttgaacgt
     4681 taaatgttcc atctgggttc accccagtta ctactgccac atgaccatat ggtgaggttc
     4741 gagctgtagt agacgttcca tatcctgtaa aatggtcagt aaatcctgaa gcaataactc
     4801 ctggagccaa atttgttcga cgcaagactc cttgactcac accatttgtt ccccaataac
     4861 ctccattacc atttactatg tttgctatat atattccttt agcggcaaag tactgccaaa
     4921 caaaatcggt acagcctccg cctgcataag gatggttctg ttgcaaagtt ccccataaca
     4981 cctgatttat tgtaaaatgt aataaaaaag aatgcgagga caatagatga atcattttaa
     5041 gggcaaacaa ttccaaaaag acgtgattat tatctctgtt gggtattatc ttcgctataa
     5101 tttgagttat cgtgatgttc aagagatgtt atatgatcgt ggcattaacg tttctcacac
     5161 aaaaatttac cgttgggttc aagaatatgg taagctgatc tatcaaatct ggaaaaagaa
     5221 aaatagacag tccttttatt cgtggaaaat ggacgaaact tatattaaaa ttaaagggaa
     5281 gtggcattat ctctatcgtg caatcgactc agaaggcatg acactggata tttggctaag
     5341 acgaaagaga aatactcagt ctgcttatgc tttctttaaa cgactataca agcagtttgg
     5401 ggaaccgaaa gttatcgtga cagataaagc accatcaatt gctagtgctt ttaggaagtt
     5461 acaaaagcag gggctataca gcgaaactga gcaccggaca gtcaagtatc tcaataactt
     5521 aattgagcaa gatcatagac ctgttaaacg tcgaaataaa ctttatcaaa gtcttcgtac
     5581 tgcatcaact acgattaagg gcatagaagc attgagaggc atatacaaaa agaaccgaag
     5641 aaatggaacg ctcttcggct tttcgacatc tactgaaatt aaagttttga tgggtatacc
     5701 agcatagtca aagtatcaga atgggacttt tgtactcagt attgaaactt tgcaacagaa
     5761 cccatttttg cgtttgactt gtaagaacag gattgaatga agccatactg ttcgcatatt
     5821 tttagtgaat gaacaaacaa aatacgagag attttttgtt cgttcatcca tggttttaga
     5881 aaaaagaggg acgatttcgg aagacgaaaa tcgtctcttt ttttcttctt tttgtatgac
     5941 aaaaagaaag atcttttgcc cattttattt ttataaaatg ggtaggtggc gtttgcgtaa
     6001 agcaaatcga cacaatccaa aggggataaa aggggaaagt gaaacttccc ccttttcaag
     6061 ccacattgta atacaagaac gaagtgcttt gtattacaat gtgatagctt gcagtattta
     6121 tggatttata ttttctattt tgttataatg attgtaaccg aatagggcgc aatgcttatt
     6181 acaaaatcaa tgacaaaggg cgattgagga atgagcgctg gggcatttta tctttgagga
     6241 ggctatttat ggatcagaaa gaagtatcac aaaatcaaac aaagtacatt caatttagat
     6301 tatctgaaga acaatacaat aagctgaaaa tttcaggaga aacatatgga ctctctccga
     6361 atctttatgc gaaaaaatta gcacaaaaat ctcatttaaa aaaaccttat ctcgaacatg
     6421 accaagcgaa agctttattg ctagaactct caaaacaagg aaccaattta aatcaaatcg
     6481 ccaagaaact caatcaattc gatcggatgg acaaccaaga taaagagctg atcgaggctt
     6541 tacgctatac atacggagta ctcgctcaag ctcaaaaggg gtatcaagag ttatggcaac
     6601 aattgcaaaa ataagcaatg gcgcaagtgc agcgagcgcc cttaattatg ctttaggtca
     6661 agacagaccc atgcatgaaa aaactgaaca gtggctacaa gaccatcaat tggaacgtcc
     6721 tgtggagctg acaaattgtc gggcagttgc cgtgggtgga accaacggga ttgatccctt
     6781 tatcgccaaa gaacaatttg aagtcgttcg acaacttcat aaccaaacga aagaatccaa
     6841 tcaagtcttg cgtattaccc aatctttcgc cttagatgaa ctgaatccga aagttcaaaa
     6901 agactggcaa aaagccaatg atttaggcgt tgaattagca gaaaatctct atcctaacca
     6961 tcaaagtgct gtgtatacgc atttagacgg aaaaaatcac gtcttacaca accacatcat
     7021 tgtcaataag gtcaatttaa agacaggaaa gaaattaaga gaacaaaaag gagaaagcgt
     7081 tcaacgagca cgagaaatga atgatcgact cgcttctcga gaaaactggc acattttaga
     7141 gccaccaaaa gagcgccaaa cagagacaga aaaagaacta atcgccaaaa atgagtattc
     7201 ttacatggat gatttgagag aaagaatcaa taaatcgctt caagatgtct ctgtgagctc
     7261 atatgagacg tttaaagcgc gcttatccga taatggtgta attctatcag aaagaggcca
     7321 aacgttctca tacgcctttt tagacgccaa taataagcaa agacgagcac gagaggcccg
     7381 attgggttct gattttggaa gggagaccat tttacatgag ttggaaaacc gagcaagaca
     7441 aaacgaattt agagctgttg aacaaggaga accagcgatt actccgcttg agcgagacac
     7501 tcaacaaaga gaatcagaaa ttgttagcct tgaacaagca attgaaccaa gaaaatcaga
     7561 agctctcaag cgagaatcaa caattaatcg atttattgac acaatcaaac agtttgcagg
     7621 acgagtacca gaacttactc aacgcgttac aagaaaatta aagcaaacaa aagagaaaat
     7681 cctcgatgat tttgaatgtc gcttttcaaa ggacatgaaa aatgacgagc aagaacaaca
     7741 gaaaagccta gaaaaacaag cgaatagaga cgtacagtcc gaaaagaaac caacaaaaga
     7801 tcatgatcgg gggatgagtc gatgaataaa gatgaacaac tcattgttca agtattaaat
     7861 gcttataaaa atggcaaaat tgatttcagt cacgttccag aactagaccg cttagtccgt
     7921 caagaggtca ataaagagtt tcgggactac caagaaaaaa tagaagctgt tgcgaatcaa
     7981 aaaatggagt ctgccattca agaacaactc caacgtttag aggcagaaaa cttaaaagcg
     8041 accatattga aagacattca agttgaaaaa caagcattac tcgctttgaa aaaagaactg
     8101 aatgaacaaa aagagcagat aaaagcagat cgcaaacgtg agattgtcga acgttacggt
     8161 attctgattg cgaacattgt ctgcctgttc tgtttcttag ttgtcggtat tctcatcgga
     8221 cgatggattt ataaagggat ctgggatggt tggggtttgc atattttgta tgatactgtg
     8281 attgagatac agcctaaaca tcgttatggg gcagtcgttc ttggattagg tggttttggt
     8341 ttaatcggtg ctggaattta tggcagtttt cgattaatgt ataccgcttc aacatggttt
     8401 gatcaacgtc caaaaatctt taaaagaatc tttccgaaaa aatagagagt gaggtaatcc
     8461 tgtggtttta gaaaataaat tagggttaac aaattcagca gaattagcta aacaagaaga
     8521 gttattaacg aaaaaaagag ccaaagaatt gtttgagtct ggcaaaataa aggatctgga
     8581 aattgggacg tttcaaggct tatctgatat tcatcagttt ttattccaag atatttacga
     8641 ctttgcagga aaaattcgag aagtgaatat tgcgaaagga aactttcaat ttgcgccacg
     8701 tattttttta gcgcaaacac ttgaatatat tgataaatta cctcaagaaa catttgatga
     8761 aattattgat aagtactcgg atatgaatgt ggcgcatcct tttagagaag gcaatggacg
     8821 agccactcgt atctggttgg atttaattct taaaaataaa ctacacaaaa tcgtcgattg
     8881 gaatcaaatt gataaggatg agtatttaaa tgccatgatt cgtagtacag tcagcaccaa
     8941 tgaattaaaa tatttgattc aaaaggcttt aaccgatgat ttaggaaaag aacaattctt
     9001 taaaggaatt gatgcgagtt attattacga gggctattat gaaattaaaa cagaagatct
     9061 ataaacacgt ataaacacgt ataaacacga tttaaagaaa cagactgctc aagttttata
     9121 ttgagcagtc tgtttctttt tttggatgat tttgaccttt gattttaaat ttttgaaaaa
     9181 aataaaaaaa ggcgaagcct attatatatt tatcttatat attttaatct tttattcttt
     9241 tgcgtgccca aaaagtcagc aatagtaagg ctatgcagat ttttatagca agaaaaaact
     9301 gtgtatatag caagaaaaaa ctgtgtatat agcaagaaaa aactgtgtat atagcaagaa
     9361 aaaactatat aaccttgcta tattatgata taataaaagc atagagaaaa ttcacgacga
     9421 aatgacttct ctatgcctac ttaaaatcac tcacaaagga gtaactttct atgtctagta
     9481 taccagaaaa acaagaaaat caaaagcagg tgctgacctt gaatgaactg tcaaaaagaa
     9541 aagtggtaga gcataacagc ttgattacgt ctattgctaa aatggataaa accccactga
     9601 aaatgtttga gttagcggtg tcttgtatca ataccgaaga gccaccaaaa gacaatattg
     9661 tttatctctc aaagagagac cttttcgctt tctttaaagt atccgataat gacaagcata
     9721 gtcgctttaa agaagcgatt gagaaaatgc aaaaacaagc cttttttcaa atcaaagaag
     9781 aagcaggcaa aggttttaaa tttaaaagca ttgttcctat accttacgtg gaatggacag
     9841 ataatcatga tgaagtaaaa attgaatttc atcgtgaaat tatgccttac ttaatcaatc
     9901 ttaaaaagaa ttttacacaa catgctttgt ctgacttagc agaattaaat agcaagtaca
     9961 gcattatctt atatcgttgg ttatccatga attataacca gtacgagcat tacagcgcta
    10021 aaggcggacg gcgagccgaa caagtagaaa gctaccgaaa tccgtctatt tcaattaaag
    10081 aattgcgtat catgactgat actgtaaatg attatgaacg gtttcagagt ttagaaacat
    10141 ggatattaaa aaaaccgttg gaagaaatca acgcccatac gtcttttaac gtgacttatg
    10201 acaaaatcaa aaaaggacgg agcattgact ctattgtctt tcatatcgag aagaaacggt
    10261 gtgcagacga taatagctac aaactcaatg ataaaatgta tcaagcagaa caagcaaaga
    10321 aagaagaaac agaagatagg ctagctattg aagcaatgaa aaataaatat actaagctat
    10381 tactcgataa tatgcttcta aattcttatg aaatgacaga tacagcaatt atggcaggct
    10441 tacagcaaca tgtttatcct ctctatgatg agttgaaaga cttacatgga atgaaagcag
    10501 ttcaagacca tctctcatat gttgctagta agcaagaagc ttattcaaaa cggaatattg
    10561 ctaaatacct taaaaaagcg attgaacaat acctcccaac agttagagga agtgagatag
    10621 aaaatatggc agacctattg aaaaaaatag agggtaaagc atgagtgaag atttaaaaac
    10681 cataaaagaa cttgctgagg agttagggtt tataaagcaa acaatacaat atcacgtaaa
    10741 gtttttgccg actaaaaatc gactaaaaaa ctctagtggt gctattgtct taaaccttga
    10801 agaacaaagg tttataaaaa ataggctaac taaaaatcga ccaaataacg accaaaaatc
    10861 gactaaagaa ccgactaaaa atcgaccaaa aaaagataaa gaaatatcta taaataatgt
    10921 tttactggag caactaaaaa caaaagacga acaaataaaa agtttagtgg aagcacaaaa
    10981 acaaactcaa aacctcttag accaacagca acgcctagca ttgcaggata aaaagttact
    11041 tgatgagtat aagtcagaaa tcaatgactt aaaagctctt aaaatgccta cacaagatac
    11101 aaaagtggag caagtgagta gcaaacaacc acaggaagta gaaagcgtgg cggaagaacc
    11161 aaagaaagca cctagaaagt ggtggggact atggcgaaaa tagatgattc agttaaaaag
    11221 aaagttccag aattaaggtt tccgggattt acggatgatt gggaagagcg taagttaaaa
    11281 gatatagctc aatttaatcc taaaacagtt cttccagatg aatttgagta tgtagactta
    11341 gaaagtgttg tagggactga aatgatctcg catagaactg aatctaaaga tcaagcacct
    11401 tcaagggcac agcgtcttgc acaaaaaggc gatgtgtttt atcagacggt tcgcccatat
    11461 caaatgaata actatttata tgatttgcct tatgataact atgttttctc aactggttat
    11521 gctcaaatgc gtcctaacat tgatagctat tttttattaa atagtgttca aaacaagcaa
    11581 ttcgttcaac atgttttaga tagaagtaca gggactagtt atccagcgat taattcaagt
    11641 gatttatcaa atattgaaat tcatgtaccg tccaaacttt cagaacaaca aaaaataggg
    11701 gcatttttcc aaagtataga cgacactatc actcttcatc agcgtaagtt agatttgttg
    11761 aaagaacaga aaaaaggctt tttacaaaag atgtttgttt aggggctata attaacaagg
    11821 tagcaaagcc tttggtagca aagagccaac gaacaggtgg caaaaaagcg acaaagacta
    11881 gcaaaggaaa taatgagcta attgctactc taagaagaga agtggaaaca agaactctta
    11941 gaaagtattc gtcctgaaaa tattgaactt gttggtgaga atgatcaagt taagctttat
    12001 ggtgttagat tctttaccta caacgatggc agaaatgttg agcaagaaat agaaaatttt
    12061 ccgtagaatt gtgaggtgat tgtatattgg aagatgaaag ttattcaatt ttaaatcaaa
    12121 gagctattat agatttactt atacaaggag acatattccc ctatatgact ggatctgaaa
    12181 ttcaagatat aggaataaaa tttggagtaa gttacgaggg aggtagtcgc tggcaaattt
    12241 tacaatcaat acttttttcg ttacatgaaa atgggaaaac tagtgatttc ttttactact
    12301 atctgtttaa aatgaacatt acttctgctg ttagagaagt gattgatgga aatatcaatg
    12361 ctaaaccgaa atacacaaaa gtagaagaaa tagtagaaga atcagaacaa aacaataaag
    12421 cgattgctag tgtattgagg acagatttaa ttaataagat aaacttagaa ctatcagtgt
    12481 ccgataaaaa attaattgtt ctcaatgaga aaatagccat tattgattct aaagcaaccc
    12541 ccaaagtact tgttgaccct gccgaaatca tgaatattgg ttatgtcaaa aaaattctca
    12601 acaatgctaa agaagattta ttaaataatg atttagacag cgtagtaact aaatctagaa
    12661 caataattga aacagtattc atagtaatat tacgtaagca tcagatcgat tttaaagaaa
    12721 atggaaatat tggtcattat agatctctag ttaataaaac attgggcatg aaacctgaca
    12781 ataggtggga tgaaaatgtt tcttctctaa ttagtggtct taataaaatc gttgattcta
    12841 taactaccat gcgaaatatt aatagtgatt cccatggtag ctctaatagg gtgaaaatca
    12901 atgaagctga agctgaacta attttaaatt ctgccgttaa tatttctgta tactatctaa
    12961 gagtggatgg aagaaaagaa aaaaatagtg ttaatattac gaaaacatcc taataatgaa
    13021 atttaaattt ttttgattcg attttttggg tctattttta gataataacc cctaagaaat
    13081 caaataaaaa cagcccctat aatccaaatt ctagattata ggggttgttt atttgtttta
    13141 atgatataat aaccctaaaa ggaggatgtt atgccata
//
LOCUS       DATRTN010000045        12853 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.045, whole genome shotgun sequence.
ACCESSION   DATRTN010000045 DATRTN010000000
VERSION     DATRTN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12853
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.045"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..512)
                     /locus_tag="VBE22_001266"
     CDS             complement(<1..512)
                     /locus_tag="VBE22_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEO7202731.1"
                     /translation="MSNFYKLLKEKFPRKEDIVTEMINLEAICQLPKGTEYFISDLHG
                     EYDAVDYLLRTGAGSIRAKLLDCFDWQKIVAVDLDDFCILLYYPKQKLAFDKMNLSAS
                     AYKTKLWEMIPLQIQVLKYFSSKYTKSKVRKQLPGKFAYIIEELLAEIDRNPEKKSYF
                     DTIIEKLFEL"
     gene            664..1524
                     /locus_tag="VBE22_001267"
     CDS             664..1524
                     /locus_tag="VBE22_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO7202732.1"
                     /translation="MTKLAIMSDLHIDLNHFSTFETKTLINLLKKEKIDHLHIAGDIS
                     NHFTKDTLPFINNLKKHIKLSYNLGNHDMLDLTETEIQRLDFQTYQFDKKMLLAFHGW
                     YDYSFSNNRDIKDVEKLKKTFWFDRRLKRPNNDVTIQASILKRLDEILAKVDSSNIII
                     AMHFVPHKQFTMTHPRFSPFNAFLGSQAYHDLFQKYHIKDVVFGHAHRSFGDVKIGET
                     TYHSRPLGYIREWNLTIDFVNQNPNHNPNLTWNLSKRYNAVKHLDSFENYRKKYFEDE
                     LRNSMTIFDC"
     gene            complement(1569..4253)
                     /locus_tag="VBE22_001268"
     CDS             complement(1569..4253)
                     /locus_tag="VBE22_001268"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEO7202733.1"
                     /translation="MLKEQKKSLFYTQGQEEVLTSLESSREGLSTTEAKNRLEMYGRN
                     ELEEGKKRSLIAKFFDQFKDLMIIILLVAAALSVITEGMHGLTDALIILVVVILNAAF
                     GVYQEGQAEAAIEALKDMSSPIARVRRDGHTIEVDSKELVPGDLVMLEAGDVVPADLR
                     LLEAASLKIEEAALTGESVPVEKDISQVVAEDAGIGDRVNMAYQNSNVTYGRGYGVVT
                     NTGMYTEVGKIADMLANADESETPLKQSLVQLSKLLTYLIVIIAVITFLVGIFVRKEG
                     WIEGLMTSVALAVAAIPEGLPAIVTIVLSMGTKTLAKRNSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGVLQSSSEEISVDNNTLRIMNFSNDTKIDPSGKLIGDP
                     TETALVQFGLDKNFDVREVLKNEPRVAELPFDSDRKLMSTIHKESDGRYFIAVKGAPD
                     QLLKRVTKIEDNGLVRDITAEDKEAILNTNKELAKQALRVLMMAYKYETQIPTLETDI
                     VESDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDA
                     NDTEDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKS
                     IQYLLSANMAEVFTIFFATLLGWDVLAPVHLLWINLVTDTLPAIALGVEPAEPGVMTH
                     KPRGRQSNFFDGGVMGAIIYQGILQTILVLGVYGWALMYPEHAGYRMIHADALTMAFA
                     TLGLIQLVHAFNVKSVYQSIFTVGAFKNRTFNWSIPVAFILLMVTIVVPGFNKLFHVT
                     HLSSTQWLTVVIGSLLMVVLTEIVKFIQRKLGQDEKAI"
     gene            complement(4597..4995)
                     /locus_tag="VBE22_001269"
     CDS             complement(4597..4995)
                     /locus_tag="VBE22_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEO7202734.1"
                     /translation="MREVMKLEINNNIQIDNETEMIHEIHDCQFIEKGSYVYLNYINA
                     EGERVVIKANHEELLMTRFSSPKSVMRFHRETPALVNIPTPLGVQHLITETSHYQFDL
                     SQQRLHIDYVLKQTETGDCFANYELRIQWY"
     gene            5059..6375
                     /locus_tag="VBE22_001270"
     CDS             5059..6375
                     /locus_tag="VBE22_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO7202735.1"
                     /translation="MNEKVFRDPVHTYIHVNNQVIYDLINTKEFQRLRRIKQTSTTSF
                     TFHGAEHSRFSHCLGVYELARKVTEIFDEHYPDLWNKNESLLTMAAALLHDIGHGAYS
                     HTFERLFNTDHEAYTQEIITNPTTEINAILRKVAPDFPDKVASVINHSYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYYTAASYGQFDLTRILRVIRPTDSGIAFARNGMHAVEDYIVS
                     RFQMYMQVYFHPASRAMELLLQNLLKRARFLFDTHRDFFEQTSPNLIPFFTDQYDLQD
                     YLALDDGVMNTYFQSWMQADDNILADLANRFINRKVFKSITFEEADKENLVKMKELVS
                     QVGFDPDYYTGVHANFDLPYDVYRPEHSNPRTEIQIIQKNGQLAELSSLSPIVKALTG
                     SNYGDQRFYFPKEMLTLDSLFSSTKEEFQSYITNEHLTLTKDDSHS"
     gene            6446..7258
                     /gene="yidA"
                     /locus_tag="VBE22_001271"
     CDS             6446..7258
                     /gene="yidA"
                     /locus_tag="VBE22_001271"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEO7202736.1"
                     /translation="MTSIKLVAVDIDGTLLNSKREITPEVAKAVQEAKSKGVKIVIAT
                     GRPIIGVQDLLEELKLNEEGDYVITFNGGLVQDTATGDDIIKETLTYEDYLDFELLAR
                     KLGVHMHAITKEGIYTANRDIGKYTIHEATLVNMPLFYRTPEEMGDKEIIKLMMIDQP
                     DILDAAIAKIPKKVLDNYTIVKSTPFYLEILPKNVNKGTALLHLAEKMGLTVDQTMAI
                     GDEENDRAMLEVVGNPVVMQNGNPELKKIAKYITKSNEESGVAYALREWVIN"
     gene            7243..8478
                     /locus_tag="VBE22_001272"
     CDS             7243..8478
                     /locus_tag="VBE22_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7202737.1"
                     /translation="MGNKLMYHVTVGISEKEYDAFAIASSQTNLLHSSKWAQVKSNWQ
                     NERLGFYKGDQLVAVASILIKSLPLGFTMLYIPRGPIMDYSNKELVGFVLKTLKNFGR
                     KKRAVFAKFDPALLLRQYHLKEENVAEEIDESRQAIDNLKSAGAQWIGPTKAISETIQ
                     PRFQANIYTKANIEENFPKHTKRLIKDAKHRGVQIYRANIDDLPKFATVVALTENRKG
                     VALRNENYFHQLMTIYGEDAYLYLAKVNLPKRLAQFKEQLLQIQKDLSETPSHQKSRL
                     TRLNQQEASVKQYILEFQEFSKKYPDEPVIAGILSIRFGNVLEMLYAGMDDSFRKFYP
                     QYLLNARVFEDAFKNGIASANLGGVEGSLNDGLTKFKSNFNPMFEEYIGEFNLAINPL
                     LYKLANLAYTIRKKQRHSH"
     gene            8491..9702
                     /locus_tag="VBE22_001273"
     CDS             8491..9702
                     /locus_tag="VBE22_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7202738.1"
                     /translation="MYREITAVEHDRFVSESNQTNLLQSSNWPKVKDNWGSQLLGFFD
                     GETQIASASILIKSLPLGFSMLYIPRGPIMDYSNLDIVTKVLKDLKAFGKKQRALFIK
                     CDPLIYLKMVNAKDFENSPDEKEGLIAIDHLQRAGADWTGRTTDLAHTIQPRFQANLY
                     ANQFGLDKMSKKTRQAIRTSKNKGVDIQFGSHELLEDFAELMKKTEDRKGINLRGIDY
                     YQKLLDTYPNNSYITMASLDVAKRLEKIEKECQIAQSERIKSLELNREKKVKQHQDTI
                     DRLNKEIDFLKEAQKAYDRDIVPLAATLTLEFGNTSENIYAGMDDYFKSYSAPIYTWF
                     ETAQRAFERGNIWQNMGGIENDLSGGLYHFKSKFEPIIEEFIGEFNIPVNRLLYKASN
                     YVYALRKKRNS"
     gene            9721..10956
                     /locus_tag="VBE22_001274"
     CDS             9721..10956
                     /locus_tag="VBE22_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7202739.1"
                     /translation="MALKELTAKEFESYSGNYDLQSFMQTPEMAKLLKKRGYDITYMG
                     YQIDGKMEIISIVYTIPMTGGLHMEVNSGPAHSNSKYLKHFYKELQNYAKSQGTLELL
                     IKPYDTYQEFTGEGEPKGAPNTYLIDDLTSIGYHHDGLHIGYPGGEPDWHYVKNLKGI
                     TPQNLLKSFSKKGRPLVKKAMSFGIKIRVLKREELHIFKDITSSTSDRRDYMDKSLDY
                     YQDFYDSFGDKAEFVIATLNFREYDHNLQLNAKKLEEQIAVLDNRHQNNTDSAKYHRQ
                     RTELVNQLASLDKRRKEVEPFIQKFGNQDVVLAGSLFIYSPKETVYLFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRQSTFYNFLGIQGNFDGSDGVLRFKQNFNGYIVRKMGTFRYY
                     PNPLKYKSIQLLKKILRRT"
     gene            11143..12075
                     /locus_tag="VBE22_001275"
     CDS             11143..12075
                     /locus_tag="VBE22_001275"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEO7202740.1"
                     /translation="MVSLKTEIAGFSFDNCLMNAAGIYCMTKEELLAIENSEAGSFVT
                     KTGTLEAREGNPQPRYADTDWGSINSMGLPNKGIDYYLDFVTELQDQDNSKNHVLSLV
                     GLSPEETHIILKKVENSSYNGLIELNLSCPNVPGKPQIAYDFEMTDLILSEIFSYYQK
                     PLGIKLPPYFDIVHFDQAATIFNKYPLAFINCVNSIGNGLVIDDETVVIKPKNGFGGI
                     GGDFIKPTALANVHAFYKRLNPSIKIIGTGGVKNGRDAFEHILCGASMVQIGTALQKE
                     GPEIFQRVSRELKEIMADKGYQSLEDFRGQLNYL"
     gene            complement(12119..12316)
                     /locus_tag="VBE22_001276"
     CDS             complement(12119..12316)
                     /locus_tag="VBE22_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202741.1"
                     /translation="MSEKAILLIILIAFVIIAIIKIALLKLYMNTIKPKVDNEGNIIT
                     KPVKDDLTPEEREEREFDKDW"
     gene            complement(12513..12788)
                     /locus_tag="VBE22_001277"
     CDS             complement(12513..12788)
                     /locus_tag="VBE22_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEO7202742.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFAAVADYLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
BASE COUNT         4280 a         2439 c         2093 g         4041 t
ORIGIN      
        1 tctaattcaa ataatttttc aataatagta tcaaaatagc ttttcttctc tgggttacgg
       61 tcaatctcag ctaaaagttc ttcgataatg taagcaaact tacctggcaa ttgcttacgt
      121 actttagatt ttgtatattt actagaaaaa tattttaaaa cttgtatttg taacggaatc
      181 atttcccata acttcgtttt ataagcactt gctgacaagt tcattttatc gaaggctaat
      241 ttttgctttg ggtaataaag cagtatacaa aaatcatcta aatcaacagc aactattttc
      301 tgccaatcaa agcagtctaa taatttagct ctaatagacc ctgcgcctgt ccgtaagaga
      361 taatcaacag catcgtattc cccgtgtaaa tcactaataa aatattccgt ccctttgggt
      421 aattgacaaa tagcttctaa attgatcatt tctgttacga tatcttcttt tcgaggaaat
      481 ttttctttca agagcttata aaaattagac atgagacctc cttaaggcac cttaaatata
      541 aagcttctaa atacacttat gaaagcgcat tctacaatga catatctatt atagaatgac
      601 tctaaaacta tttcaaaaat ttactgttta actctttgaa ctacaatatg gtacaatacc
      661 cttatgacca aacttgctat catgagtgat ttacatattg acctcaatca tttttctacc
      721 tttgaaacga aaacgttaat taatttgctt aagaaagaaa aaattgatca cctccatatc
      781 gctggagata tatctaatca ttttactaaa gatacacttc cctttatcaa taacttaaaa
      841 aagcatatta aacttagcta taatttagga aaccatgata tgcttgattt aacagaaaca
      901 gaaatccaac gccttgactt tcaaacctat caatttgaca aaaaaatgct actagctttt
      961 catggctggt atgattacag cttttcaaat aatagagaca tcaaggatgt tgaaaaatta
     1021 aaaaagactt tttggtttga tagacgttta aaacgtccaa ataatgatgt aactatccaa
     1081 gcatcaattt taaaacgctt agacgaaata ttagcaaaag tcgattcttc aaatatcatt
     1141 attgctatgc actttgttcc ccataaacaa ttcacgatga ctcatcctag attctctcct
     1201 tttaatgctt ttttggggag tcaggcttat catgatttat tccaaaaata tcatatcaaa
     1261 gatgttgttt ttggtcatgc acaccgctca tttggcgacg ttaaaattgg agagactact
     1321 tatcattcta gaccacttgg atatattaga gaatggaatt taactattga ctttgtcaat
     1381 cagaatccca atcataaccc taatttaacc tggaatctta gcaaaaggta taatgccgtt
     1441 aagcatttgg atagttttga aaactatcga aaaaaatatt ttgaagatga attacgcaac
     1501 tctatgacaa tatttgattg ctaaaaaact cacttagata ttctaagtga gtttttttat
     1561 cagctaaact aaatagcttt ttcgtcttgt cctaatttac gttggataaa tttaacaatt
     1621 tccgttaaca ctaccatcaa taaacttccg ataactacag ttaaccattg tgttgaactt
     1681 aaatgagtta catggaaaag tttattaaat ccaggaacaa caatagtcac catcaaaaga
     1741 atgaaagcta ctggaataga ccagttaaag gttctatttt taaaagctcc aactgtaaag
     1801 attgattggt aaactgattt aacgttaaat gcatgtacca attggattaa accaagcgtt
     1861 gcaaaggcca tcgtaagggc atctgcgtga atcatgcgat aaccagcatg ttctggatac
     1921 atcaatgccc aaccatagac accaagtact aagatagttt gtaaaatacc ttgatagata
     1981 atagctccca taacaccacc atcaaagaag tttgactggc gaccacgagg tttatgtgtc
     2041 ataactcctg gttcagctgg ctcaactcca agagcgatag ctggaagagt atctgttacc
     2101 aagttaatcc aaagaaggtg aaccggtgca agtacatccc atcctaataa cgttgcaaag
     2161 aaaattgtaa atacctctgc catgttggct gaaagaagat attgaataga tttttggata
     2221 tttgagaaga ctttacgtcc ttcctcaaca gccacaataa tagttgcaaa attatcatca
     2281 gcaagaacca tatcagaagc acctttggaa acttctgtac cagttattcc cataccaata
     2341 ccaatatctg ctgtttttag cgatggagca tcattaacac catcaccagt catggcaacg
     2401 actttgccat cgttttgcca agcttttacg atacgcactt tatgttcagg agaaacacgt
     2461 gcatagacag aatattgttt aaatactttt tgaaactctt cgtcacttaa ttcattaagt
     2521 tcagcacccg taaagacatg atcttctgtg tcatttgcat caataatacc tagacgcttt
     2581 gcaatagctt ctgcagtatc ttgatggtcc ccggtaatca taatcggacg aattcctgct
     2641 tctttagcaa ctcgaacagc ttctgccgct tctggacgct cagggtcaat cattcccact
     2701 aaacctgaga atactaaatc tgactcaaca atatcagtct ctaaagttgg aatttgtgtt
     2761 tcatatttat aagccatcat caagacacga agagcttgtt ttgctagttc tttattggta
     2821 tttaggatag cctctttatc ttcagccgtg atatcacgaa ccaagccatt atcttcaatt
     2881 ttagtaactc gttttaataa ttgatcagga gcacccttga ctgcgataaa gtagcgacca
     2941 tctgactctt tatgaatcgt agacattaac ttacgatctg aatcaaatgg taactcagct
     3001 acacgtggtt catttttcaa aacttcacgg acatcaaaat tcttatctaa accaaattgt
     3061 accagtgcag tctctgttgg gtcaccaatt aattttcctg aaggatcaat cttagtatcg
     3121 ttggagaaat tcataatacg taaagtgtta ttatcaactg aaatttcttc cgaagatgat
     3181 tggagaacgc cattagtata cactttttcg acagtcattt gattcattgt cagagtacca
     3241 gtcttatccg atgcaatgat ttctgtagaa cctaaagttt caactgcagg gagtttacga
     3301 acgatagaat tgcgtttagc aagagttttt gttcccattg ataagacaat cgtaacaatt
     3361 gcaggaagcc cttctggaat agctgcaact gctaaagcaa ctgaagtcat caacccttca
     3421 atccaacctt ctttacggac aaatatcccc actaagaaag taataaccgc aataatcacg
     3481 attaagtaag tcaatagttt agaaagttga actaaacttt gtttcaatgg tgtttcagat
     3541 tcatcagcat ttgccaacat gtccgcaatt ttaccaactt cagtatacat ccctgtgtta
     3601 gtaacaacac catatcctcg accatacgtg acatttgaat tttgatacgc catattcaca
     3661 cggtcaccaa tacctgcatc ctctgctact acttgagata tgtctttctc aacaggtact
     3721 gactcaccag ttagagctgc ttcttcaatt tttagagacg cggcttctaa aagtcgtaaa
     3781 tctgcgggaa caacatctcc tgcttctaac ataacgaggt caccaggaac tagttcttta
     3841 gaatccacct caatagtatg accatcacga cgaacacgcg caattgggct tgacatatct
     3901 ttgagggctt caatagctgc ttcggcttgc ccctcttgat aaacaccaaa agctgcattt
     3961 aagataacaa ctaccaagat aatcaatgca tccgttaagc catgcatacc ttccgtaata
     4021 acagagagag ctgcagcaac cagtaagata ataatcatta aatctttgaa ttggtcaaaa
     4081 aacttagcta ttagactacg ttttttccct tcttcaagct cattgcgacc atacatctct
     4141 aagcgatttt tcgcttctgt tgttgataat ccttcacgag aagattctaa agacgttaga
     4201 acctcttctt gaccttgtgt gtaaaacaag gattttttct gttcttttaa caaaaccttt
     4261 cctcctttgg tctctccaca aacaaaaaga gacctgtttt tatgaaaaca agtctcgctg
     4321 tttaagacaa cgccggaaat ttctttcgta atgacgacat tgccacggta aaccgtctgc
     4381 tactcccttg atatgttaat cattgtaaca gaaaaaaatc catttttcaa ttaattatcc
     4441 ttgctatcac taataatctc aagggaaaaa acgaaattca tctagtcgaa ataaaccata
     4501 ctgcagttta tcaaataaat ggctctcata aagagatccc agcgtcttca gtagacgaaa
     4561 ttcataacga ctactctttt gttaatggta gttatcttaa taccattgaa tccttaattc
     4621 ataatttgca aaacagtctc ctgtctccgt ttgcttaagg acataatcga tatgcaacct
     4681 ttgctgtgac aaatcaaact gatagtgact tgtctctgta atcaaatgtt gaacaccaag
     4741 tggagtgggg atattaacca aagcaggcgt ttcacgatga aaacgcatta cagacttagg
     4801 acttgaaaac ctcgtcataa gcaattcctc atgatttgcc ttaataacta ccctctcgcc
     4861 ctcagcatta atataattca aataaacgta acttcctttt tcaataaatt gacaatcgtg
     4921 aatctcatga atcatttcgg tttcgttgtc aatttgaatg ttattgttaa tttctagttt
     4981 catgacttct ctcacttctt ttatccttct attctaccaa tttctgactc aagtttcaat
     5041 ttttgttata atatatatat gaatgaaaaa gtatttcgtg accccgttca cacttatatc
     5101 catgtaaata atcaagtcat atatgacttg attaacacta aagaatttca acgtttacga
     5161 cgcattaaac aaacctcaac gacttctttt accttccatg gtgcagagca tagtcgtttt
     5221 tcacattgtc ttggagttta tgaactggct cgtaaggtta cagaaatctt tgatgaacat
     5281 taccctgacc tttggaataa aaatgagtcc ttgctaacga tggctgccgc cctactacat
     5341 gatatcggac atggcgcata ttctcataca tttgagcgtc tcttcaatac tgatcatgaa
     5401 gcttacactc aggaaatcat cacaaatcct accacagaga ttaatgctat tttacgaaaa
     5461 gtggctcctg atttccctga taaagtagct agtgttatta atcatagcta ccccaataaa
     5521 caagttgttc aattaatttc tagtcaaatt gattgtgatc gaatggacta tcttctcaga
     5581 gattcttact acactgctgc tagctatgga caatttgatt taacacgaat tttacgcgtt
     5641 attagaccaa ccgattctgg aattgctttt gctcgtaacg ggatgcacgc tgtggaagat
     5701 tatattgtta gtcgcttcca aatgtatatg caagtttatt tccatccagc cagtcgtgct
     5761 atggaattac tcttacaaaa tttactaaaa cgtgctcgct tcttatttga tacccatcgc
     5821 gatttttttg aacaaacttc tccaaatctt atccctttct tcacggatca atatgattta
     5881 caagattatt tagccctaga tgatggggta atgaatactt acttccaatc ttggatgcaa
     5941 gctgatgata acattttggc agatttagct aatcgtttta tcaatcgaaa agtctttaaa
     6001 tcaattacct ttgaagaggc tgacaaagaa aatctagtta aaatgaaaga actagtctca
     6061 caggttggtt ttgatcccga ttattacact ggtgtccatg ctaattttga cttaccttac
     6121 gatgtttatc ggcctgaaca ttcaaatcca cgaacagaaa ttcaaattat ccaaaagaat
     6181 ggacaacttg ctgaattatc aagcttatca ccgattgtaa aagcattaac tggatctaat
     6241 tatggtgatc aacgttttta ttttccaaaa gaaatgttga cattagatag cctattttca
     6301 agtacaaaag aagaatttca atcttacatc acaaatgagc atttgacact cacgaaagat
     6361 gatagccatt catgacaaac cctttatttt ttagtaagat ataaggtgag tattttagga
     6421 tattgaataa agagtgggag ataaaatgac ttcaattaaa cttgttgccg tagatattga
     6481 cggcacccta ttaaatagta agcgtgaaat tacacctgaa gtggctaaag ctgttcaaga
     6541 agcaaaatca aaaggcgtta aaattgttat tgctacggga cgtccaatca tcggagttca
     6601 agatctttta gaagaattga aactcaatga agagggggat tatgttatca cctttaacgg
     6661 cggtctagtt caagacacag ccacaggaga tgacatcatc aaagaaacct taacctatga
     6721 agactatctg gatttcgaac tgttagcgcg taaattaggc gtccacatgc acgccattac
     6781 aaaagaagga atttacacag caaatcgtga tattggtaaa tatacaatac acgaagcaac
     6841 tttagttaat atgccgctct tctatcgtac tcctgaagaa atgggagaca aagaaatcat
     6901 taaactaatg atgattgatc aacctgacat cttagatgca gcaattgcta aaattccaaa
     6961 aaaagttctc gataactaca ccattgtaaa atcaactcct ttttatttag aaattttacc
     7021 caaaaacgtt aataaaggca ctgcacttct ccaccttgca gagaaaatgg gattaactgt
     7081 agatcaaaca atggctattg gtgatgaaga aaatgaccgt gctatgcttg aagttgttgg
     7141 aaatcctgtt gttatgcaaa acggcaatcc tgaactaaaa aaaatagcaa aatatatcac
     7201 caaatcaaac gaggaaagtg gcgtagccta cgcactacgt gaatgggtaa taaactgatg
     7261 tatcatgtta ccgttggaat atccgaaaaa gaatatgatg cctttgctat tgcatcgtct
     7321 caaaccaatc tcctccactc ttcaaagtgg gcacaagtaa aatcaaattg gcaaaatgaa
     7381 cgtttaggtt tttataaagg tgaccaatta gttgctgtag ctagcatctt aattaaatca
     7441 ttaccacttg gttttacaat gctttatatt ccacgtggac ctattatgga ctacagcaat
     7501 aaagaattag ttggctttgt gttaaaaaca ctaaaaaact ttggacgaaa gaaacgtgct
     7561 gtttttgcaa aattcgatcc agcacttctt ctacgtcaat atcaccttaa agaagagaat
     7621 gtcgccgaag agatagatga aagtagacag gcaattgata atctcaagtc agcaggtgcc
     7681 caatggatag gtccaacgaa agccatttcc gaaaccatcc aacctcgttt tcaagcgaat
     7741 atctatacca aagcaaatat agaagaaaac tttccaaaac acactaaacg tctgataaaa
     7801 gatgcaaagc atcgcggtgt tcaaatttat cgtgccaaca ttgatgattt acctaaattc
     7861 gccacggttg tagcattaac cgagaatcgt aaaggtgtag ctttacgtaa tgaaaactat
     7921 ttccatcaat taatgacaat ttatggagaa gatgcctatc tctatttggc taaagtcaat
     7981 cttcctaaaa gattagcaca atttaaagaa caacttcttc aaatccaaaa agatttatca
     8041 gaaactccat cccatcaaaa aagtcgcctg acaaggctaa atcaacaaga agcttctgtt
     8101 aaacaatata tccttgaatt tcaggagttt agcaagaaat atccagatga gcctgtcatt
     8161 gcaggaattt tgtccattcg ttttggcaat gtcctggaga tgttgtatgc tggtatggat
     8221 gatagcttcc gtaaatttta tccacagtat ctcttgaatg ctagggtgtt cgaagatgct
     8281 ttcaaaaacg gtattgcatc tgctaatcta ggcggtgtcg agggaagtct taatgatggt
     8341 ttaacaaaat ttaaaagtaa cttcaatcca atgtttgaag aatatattgg cgagtttaat
     8401 ctagcaatca atccattatt atataaattg gccaacctag cctatactat tcgaaaaaaa
     8461 caaagacact ctcactaaga aaggaaactc atgtatagag aaattaccgc tgtcgaacac
     8521 gatcgctttg tgagcgaatc caaccaaaca aacctacttc aatcttctaa ttggcccaaa
     8581 gtaaaagaca actggggtag tcaattactt ggcttttttg acggtgaaac ccaaattgcc
     8641 agcgctagta ttctcatcaa atcacttcct cttggcttct ccatgctata tattccgcgt
     8701 ggaccaatca tggattactc caatctagat attgtaacta aggtccttaa ggaccttaaa
     8761 gcttttggca aaaaacaaag agctctcttt atcaagtgtg atcctctcat ctatttaaaa
     8821 atggtcaatg ccaaagattt tgaaaattcg cccgatgaaa aagaagggtt aatcgccatt
     8881 gatcatttac agcgtgcagg tgctgattgg actggtcgga caacagattt agctcatact
     8941 atccaaccac gttttcaggc aaacctatat gctaatcaat ttggacttga taaaatgtct
     9001 aaaaaaactc gtcaagctat tcgaacttcc aaaaataagg gagtagatat acaatttggt
     9061 agccatgaac tacttgaaga ttttgcagag ttaatgaaaa aaacggaaga ccgtaaaggg
     9121 ataaatctta gaggaattga ctactaccaa aaactccttg atacgtatcc taataactca
     9181 tacatcacaa tggcttcgct agatgttgca aaacgattag aaaaaataga aaaagaatgt
     9241 cagatagctc aatctgaaag aataaaatca cttgaactta atcgtgagaa aaaagttaaa
     9301 caacaccaag atacgattga tcgattaaat aaagaaattg attttctcaa agaagctcaa
     9361 aaagcctatg accgagacat tgttccattg gctgcaaccc tcactctaga atttggtaat
     9421 acatcagaaa atatttatgc tggtatggac gattacttca aatcttattc tgctcctatt
     9481 tacacttggt ttgaaacagc tcaaagggct tttgaacgtg gaaatatttg gcaaaatatg
     9541 ggggggattg agaatgactt atctggtggt ttatatcatt ttaagtctaa gttcgaacca
     9601 atcattgagg aatttattgg agaatttaac attccagtga acagattgct ttataaagcc
     9661 tctaattatg tctatgcctt acgtaaaaaa cgtaatagct agttaggaaa ggatactttt
     9721 atggcgctta aagaattaac agctaaagag ttcgaaagtt attcaggaaa ttacgattta
     9781 caatcgttta tgcaaacccc tgagatggca aagcttctaa aaaaacgtgg gtatgacatt
     9841 acctacatgg gctaccaaat cgatgggaaa atggaaatca tcagtattgt ttataccatc
     9901 ccaatgactg gtggacttca tatggaagtc aattctggtc cagcccattc caattctaaa
     9961 tatctgaaac atttttataa agaactacag aactatgcca aatctcaggg cactttagaa
    10021 ttattaatta aaccttatga tacctaccaa gagtttactg gtgaaggtga gcctaaaggt
    10081 gcaccgaaca cttatcttat tgatgacctg actagtatag gttatcacca cgacgggctt
    10141 catattggtt atcctggcgg tgaacctgat tggcattatg ttaaaaatct aaaaggtatc
    10201 actcctcaaa atcttcttaa atctttctct aaaaaaggac gccccttggt taagaaagcc
    10261 atgtcttttg gtataaaaat acgtgtcctc aagcgggaag aactacatat ctttaaggat
    10321 attacttctt ctacttctga tcgccgggac tatatggata aatctctaga ttattatcaa
    10381 gatttttatg atagtttcgg agataaggcg gaatttgtca ttgcaactct aaattttagg
    10441 gaatatgatc ataacctaca attaaatgca aagaaattgg aagaacagat tgcagtcctt
    10501 gataatcgcc atcaaaataa tacagattct gccaaatacc atcgtcaacg tactgagctt
    10561 gtcaatcaac tagctagttt agataaacgt cgcaaagaag tcgaaccttt tattcaaaaa
    10621 tttggaaatc aagatgttgt tctagctggt agcctcttta tttatagccc taaagaaact
    10681 gtctatcttt tctctggctc atacacagaa tttaataaat tttatgcgcc tgcagttctt
    10741 caagaatatg ttatgcaaga ggctttaaag cgtcaatcaa ctttctataa ttttttaggt
    10801 attcaaggaa attttgatgg ttcagatggc gtacttcgct ttaaacagaa ttttaatggt
    10861 tacattgttc gtaaaatggg tactttccgc tactatccaa atccattaaa atataagagt
    10921 attcaattac taaagaaaat cttacgccga acataaaaaa taagcttaca taagcttatt
    10981 ttttatgcat tatagaaagc aacatttatt tctttactat tattgtttat acaaataata
    11041 acgttcgtta tttagcgaaa ttttattacc aatatcatta ttttcttgcc gctctgttaa
    11101 tcaagtgata aaatagtaaa aatttatctt caggaggata ttatggtatc gctaaaaacg
    11161 gaaattgctg gttttagttt tgacaattgt ttgatgaatg cagcgggtat ttattgtatg
    11221 actaaggaag agttattagc tattgaaaat tctgaagctg gttcctttgt tacaaaaaca
    11281 ggtacccttg aagctagaga gggaaaccct caaccacgtt atgcagatac tgactgggga
    11341 tcaattaatt ctatgggact ccctaataaa ggtattgact attatcttga tttcgtgact
    11401 gaattacaag atcaagacaa tagcaaaaat cacgtcttat cattagtagg tttatcacca
    11461 gaagagacac atattattct taaaaaggtt gaaaacagta gttacaatgg cctaatagag
    11521 ttaaatttat cgtgcccaaa tgttcctgga aaacctcaaa ttgcttatga ttttgaaatg
    11581 actgacctca tcctttcaga aatctttagc tattatcaaa agcccttggg tatcaaatta
    11641 ccaccttact ttgatatcgt tcactttgat caagcagcta caatttttaa caaatatcct
    11701 ttagctttta ttaactgtgt taattctatc ggaaatggac ttgttattga tgatgaaact
    11761 gtcgttataa agcctaaaaa tggctttgga ggtatcggtg gtgattttat caagcctacc
    11821 gccttagcta acgtccatgc tttctacaaa cgtttaaatc cttcaattaa aattatcgga
    11881 acaggtggtg ttaaaaacgg ccgtgatgcc tttgaacaca ttctatgtgg agctagtatg
    11941 gtccaaatcg gaaccgcttt acaaaaagaa ggaccagaaa tttttcagcg tgtaagcaga
    12001 gaattaaaag aaataatggc tgataaaggc tatcaatcat tggaggattt tcgaggacaa
    12061 cttaattacc tttaaaaata ataaaagagc aaacaaggtt tgctctttta tattgatttt
    12121 accaatcttt atcaaattca cgttcttcac gttcctcggg tgttaaatca tcctttacag
    12181 gctttgttat gatgtttcct tcattatcta ctttaggttt tatcgtattc atatataact
    12241 ttaacagagc tatctttata atagcgataa taacaaaagc aataaggata attaatagta
    12301 tagctttttc tgacattctc tttcgcaacc tctcttttaa cttatctttt aggaattata
    12361 acatcacagt taagataatt caagggtatg ctctaacaca tttgaaactc taaattcctt
    12421 tgagctctct agtcgtttat tgagtattgg atataaaaaa gaaccttgtc gctatcaata
    12481 aacattgata tgacaaggtt tttctagacg aattatttaa cagcgtcttt aagagcttta
    12541 ccagctttaa atgctggaac ttttgatgct gcaatttcga tttctgcacc agtttgtggg
    12601 ttacgacctt tacgagctgc acgttcacga acttcaaagt taccaaaacc gattaattgt
    12661 actttttcac cttcagcaag gtaatctgct acagctgcaa aaactgcatc tactgctgct
    12721 gctgaatctt ttttagttaa ttctgtagct tctgctactt ttgcaattaa atcttgtttg
    12781 ttagccatgt taacaaatcc tccaaataaa ttctaaactt atacaacata agttttaaca
    12841 gattatatga tat
//
LOCUS       DATRTN010000046        12813 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.046, whole genome shotgun sequence.
ACCESSION   DATRTN010000046 DATRTN010000000
VERSION     DATRTN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12813
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.046"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            24..464
                     /locus_tag="VBE22_001278"
     CDS             24..464
                     /locus_tag="VBE22_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7202743.1"
                     /translation="MENPLQKARILVNQLEKYLDRYAKEYDVEHLAGPQGHLVMHLYK
                     HPDKDMSIKDAEEILHISKSVASNLVKRMEKNGFIAIVPSKTDKRVKYLYLTHLGKQK
                     ATQFEIFLEKLHSTMLAGITKEEIRTTKKVIRTLAKNMAMEDFD"
     gene            511..2328
                     /locus_tag="VBE22_001279"
     CDS             511..2328
                     /locus_tag="VBE22_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202744.1"
                     /translation="MFNIFKRLNGKEWLMIVASTILVCLAVWMDLKTPEYMSDITRML
                     QTKGTKLEALWDPGLKMLAFSFGSFLVAVMVGFLGARTAASFTSRLRSDIFNHVMDYS
                     DAEIKRFSIPSLLTRTTNDLTQLQILIVMGMQVVTRGPIMAIWALTKIWGKSDQWTGA
                     VGVAVIIVMILLSVLLLLAFPRQRQIQGFTDALNATTREALSGVRVIRAYNAEKYQTD
                     KFKRENDDLTKLNLFVYRLMSLMNPVMTVVSSGLTLAIYWIGSYLINDISVPKVTGTS
                     VSVLAAKSAITDRVDLFSNMVVFSSYAMQVVMGFMMMVAIFIIMPRALVSARRINEVL
                     TLAPTVTFKENSHIQSAQKGQIEFQDVSFHYSKDSEAVIEHVSFKADTGQTVAFIGST
                     GSGKSTLVNLVPRFYDATEGAIKLDGIDIKEYSHNDLNNKIGYIPQKAVLFSGTVRSN
                     MILGESGREELSDEEIWQALELAQAKEFIEAKEKGLDTEVAQGGSNFSGGQKQRLAIA
                     RALARHPEILIFDDSFSALDYKTDHILRHEVKEKTAAMTKLIVAQRISTIMDADLILV
                     LDQGKVVGQGTHKELLSNNEVYQEIAYSQLSKEELENEN"
     gene            2318..4072
                     /locus_tag="VBE22_001280"
     CDS             2318..4072
                     /locus_tag="VBE22_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202745.1"
                     /translation="MKTKSQKTSFFSNLSPYLKGYKLLFALAIVLATLSSLTTVIGPD
                     RLKEMTDLMVKGLAGNIDLAKIGHIAGQLAVLYVLGALVSYLASFIVTTIIQKFAQGM
                     RNAIADKINKIPLSYFDSHTQGDTLSRVTNDVDLMTQSFNQSLVTMVASTVLLIGSLI
                     MMFKTDWHLATTAIISVFSGFALSSIIMVKSQPLFKKQQMNLANISGYVEEIYSGHNI
                     VTSHNARQQAKSDFQLLNDKLHSSMWKSQFFSGIMMPLMQFIGNFGYVMVCVVGAMLA
                     IKGTITMGTIVAFMTYVRIFTQPIGQIAQGITQLQSASAAMGRVFEFLNETNMQEDKH
                     KVLNLPKFKGYVSFEHVKFGYSDNKTIIHDFTSEAQPGQKVAIVGPTGAGKTTIVNLL
                     MRFYDVNSGSISIDDINIQDMKRSQVHDAFSMVLQDIWLFEGTIKENLIFNQTTISDD
                     EVQEAAKAVGVHHFIMTLPKGYDIVLDDAVTLSVGQKQLLTIARALLKNSPLLILDEA
                     TSSVDTRTEELIQKAMDKLMEGRTSFVIAHRLSTIKNADLILVMKDGNIIEQGSHDVL
                     MSQSGFYADLYNSQFESA"
     gene            4180..4806
                     /locus_tag="VBE22_001281"
     CDS             4180..4806
                     /locus_tag="VBE22_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO7202746.1"
                     /translation="METNIYGDYGRYLPLILEDFSQRIQLENDKAKVETGYKLYEHII
                     GRIKTSDSMIEKCRRKQLPVTVDSALKAIRDSIGVRIICGFVNDIYQIVERIKSFDDC
                     RIVVEKDYIQHVKPNGYRSYHVILEIDTPYPDCLGNSEGKYYIEIQLRTIAQDSWASL
                     EHQMKYKHDIENPERIVRELKRCADEMASVDLTMQTIRQLIESGTKKE"
     gene            4808..5494
                     /locus_tag="VBE22_001282"
     CDS             4808..5494
                     /locus_tag="VBE22_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7202747.1"
                     /translation="MRVLIAEDEEQMSRVLSTAISHQGYVVDVAYDGQTAIDLANQNA
                     YDVMVMDVMMPVKTGVEAVKEIRQSGNKSHIIMLTAMAEIDDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMSRRLEDFTPNVLSLGRVTLSVGEQELQCENTIRLAGKEAKMLAFF
                     MLNHDKELSTQQLFEHVWGADKDQEDVDEGYVFIYVSYLRQKLQAISANLEIIGQEGS
                     SYKLSEINGG"
     gene            5494..6723
                     /locus_tag="VBE22_001283"
     CDS             5494..6723
                     /locus_tag="VBE22_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7202748.1"
                     /translation="MFRNLRLRFIGIAALAILVVLFSVVGVLNSANHYQTKNEIYRVL
                     TILADNNGRIPNKLEFSKELGDDLSTDAIFQFRYFSARTDAKGNVTSFDSRNIFEVSD
                     RQIKNYAKRIVSQNSDSGHITYNFSTYSYLLKKVGKNDYLVVFLDTTNQYLDNQRLLQ
                     LSIWMSLASFIVFMVIVSVLSGRVILPFVANYEKQRRFITNAGHELKTPLAIISANNE
                     LVEMMSGESEWTKSTNDQIQRLTGLINGMVSLARFEEQPDISMVDLDFSHITKDAAED
                     FKGPIIKDGKDFIMSIQPGIHVKAEEKSLFELVTLLVDNANKYCDPMGTVTVKLSRSS
                     RLRRAKLEVSNTYKNGKDIDYSKFFERFYREDESHNNKKSGYGIGLSIVTSLVHLFKG
                     SIDVNYKHDTITFVIYI"
     gene            6839..7717
                     /gene="mvk"
                     /locus_tag="VBE22_001284"
     CDS             6839..7717
                     /gene="mvk"
                     /locus_tag="VBE22_001284"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEO7202749.1"
                     /translation="MKEKFGIGKAHSKIILMGEHSVVYGYPAIAIPLKNIEVTCLIEE
                     APQLIALDMTDPLSTAIFAALDYLGKTSSKIAYHIESQVPERRGMGSSAAVAIAAIRA
                     VFDYFDEDLEADLLECLVNRAEMIAHSNPSGLDAKTCLSENTIKFIRNIGFSTVPMHL
                     NAYLVIADTGIHGHTKEAVDKVKSSGEAVLPFLKELGYLAEASEDAIHKSDSKQLGSL
                     MTKAHQSLKQLGVSSLEADHLVEVAISCGALGAKMSGGGLGGCIIALVKEKREAERLS
                     QQLEREGAVNTWTEKV"
     gene            7699..8643
                     /gene="mvaD"
                     /locus_tag="VBE22_001285"
     CDS             7699..8643
                     /gene="mvaD"
                     /locus_tag="VBE22_001285"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEO7202750.1"
                     /translation="MDGKSISVKSYANIAIIKYWGKADAEKMIPATSSISLTLENMYT
                     ETRLTTLGKDAKKDEFYISGVLQNDHEHDKMSAILDRFRQNKSGFVKIETTNNMPTAA
                     GLSSSSSGLSALVKACNDFFGTNLSQSQLAQEAKFASGSSSRSFFGPVAAWDKDSGDI
                     YKVHTDLDLAMIMLVLNDKRKPISSREGMKICTETSTTFNEWVRQSEQDYQDMLVYLK
                     NNDFQKVGQLTERNALAMHSTTKTATPAFSYLTEETYKAMDVVKKLREKGHECYYTMD
                     AGPNVKVLCLRQDLEALAAILEKDYRIIVSTTKELADG"
     gene            8636..9628
                     /locus_tag="VBE22_001286"
     CDS             8636..9628
                     /locus_tag="VBE22_001286"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEO7202751.1"
                     /translation="MVKVQTGGKLYIAGEYAILYPGQVAILKNIPIYMTALATFADNY
                     SLYSDMFNYTASLQPDKQYSLIQETILLMEEWLINFGKNIKPIHLEITGKLERYGLKF
                     GIGSSGSVVVLTIKAMAALYEIEMPSDLLFKLSAYVLLKRGDNGSMGDIACIAYEHLI
                     SYSAFDRRAVSKMIETKPLEQVLEAEWGYRITKIQASLEMDFLVGWTMQPSISKEMIN
                     IVKSTITQRFLDDTNYQVVQLLSAFKEGDKEAIKRCLEEISLLLFNLHPSIYTDKLQK
                     LKEASKGLDIVTKSSGSGGGDCGIAISFNKNDNQTLIKRWESAGIELLSKETLS"
     gene            9625..10620
                     /locus_tag="VBE22_001287"
     CDS             9625..10620
                     /locus_tag="VBE22_001287"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEO7202752.1"
                     /translation="MTNRKDDHIKYALKYQSPYNSFDDIELIHSSLPKYNVNDIDLST
                     HFAGQSFEFPFYINAMTGGSEKGKAVNHKLAQVAQATGIVMATGSYSAALKNDEDDSY
                     PTTDLYPDLKLATNIGLDKPLPAAESTVKAMNPIFLQVHVNVMQELLMPEGEREFHMW
                     RSHLKEYVDNIQCPLILKEVGFGMDLQSIKDAYDIGITTVDISGRGGTSFAYIENQRG
                     RDRSYLNTWGQTTAQSLINAQSMMDKMDILASGGIRHPLDMVKCLVLGAKAVGLSRAV
                     LELVERYPVDDVIAILNSWKEDLRMIMCALNCKKITDLRQVNYILYGQLKEANAK"
     gene            10688..10906
                     /locus_tag="VBE22_001288"
     CDS             10688..10906
                     /locus_tag="VBE22_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEO7202753.1"
                     /translation="MIKLYYEDAYDTERCQVFENPNDFIRVLMGCVTVPDNYPITKMT
                     YKDKNIAYSGTYGDLFKTFIEFDWSTLD"
     gene            complement(10952..11806)
                     /locus_tag="VBE22_001289"
     CDS             complement(10952..11806)
                     /locus_tag="VBE22_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEO7202754.1"
                     /translation="MTTFTAKFIDQEWEVPVESGRYHMIVGEFCPYAQRPQIARQLLG
                     LDKHISISFVDDVPSDIGLIFSQPEQVTGAKSLRDIYHLTDPTYKGPYTIPILIDKTD
                     NRIVCKESADMLRLFTTDFSDLHQEDAPVLFSQETASLIDNDIKDINNNFQSLMYKLA
                     FLDKQTDYDTYSKEFFTFLDQKEHLLGQRPFLLGDNLSEVDIHFFTPLVRWDIAGRDL
                     LLLNQKALEDYPNIFSWAKTLYNDFNLKTLTNPQSIKNNYYLGKFGRAVRHHTIVPTG
                     PNMVKWEK"
     gene            complement(11907..>12813)
                     /locus_tag="VBE22_001290"
     CDS             complement(11907..>12813)
                     /locus_tag="VBE22_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO7202755.1"
                     /translation="AYIFFNPKSGKDEQALAQEVKSYLIEHDFQDDYVRIITPSSVEE
                     AVALAKKASEDHIDLVIPLGGDGTINKICGGVYAGGAYPTIGLVPAGTVNNFSKALNI
                     PQERNLALENLLNGHVKSVDICKVNDDYMISSLTLGLLADIAANVTSEMKRKLGPFAF
                     VGDAYRILKRNRSYSITLAYDNNVRSLRTRLLLITMTNSIAGMPAFSPEATIDDGLFR
                     VYTMEHIHFFKLLLHLRQFRKGDFSQAKEIKHFHTNNLTISTFKRKKSAIPKVRIDGD
                     PGDQLPVKVEVIPKALKFIIPNSLP"
BASE COUNT         4179 a         1977 c         2576 g         4081 t
ORIGIN      
        1 ataactatta aaggagtatt attatggaga atcctcttca aaaagcacga atacttgtta
       61 atcaacttga aaagtattta gatcgttatg caaaagagta tgatgttgaa catttagcgg
      121 gtccacaagg acacttagtg atgcatctct ataaacatcc tgacaaggat atgtctataa
      181 aagatgctga agaaatttta cacatctcta agtctgtagc ttctaatttg gtaaaacgga
      241 tggaaaaaaa tggatttatt gcgattgttc catctaagac agataagcgt gtgaaatacc
      301 tttatttgac tcatctaggt aaacaaaagg ctacacaatt tgaaattttc ttagaaaaat
      361 tgcacagtac catgttagca ggtattacta aagaagagat acgtactacc aaaaaggtta
      421 ttagaacatt agctaaaaat atggcgatgg aagattttga ttaactattt taggataata
      481 ggataaatat gaaagtaaaa ggagtttgcg atgtttaata ttttcaagcg actcaatggt
      541 aaagaatggt tgatgattgt ggcgagtact atattagtgt gtctagcggt ttggatggac
      601 ctaaagactc cagaatatat gtctgatatc acacgtatgt tgcaaactaa aggtacaaag
      661 ttagaggctc tttgggatcc tggactaaaa atgcttgcct tttcatttgg tagtttttta
      721 gtagcagtaa tggtaggttt tttaggtgct aggacagcag ctagttttac tagtcgatta
      781 cgttcagata tttttaacca tgtgatggat tattctgatg cagaaattaa gcgattttct
      841 atcccctctc tattaacgcg cacaactaac gacttgactc agctacagat ccttattgta
      901 atgggaatgc aggttgttac ccgtggacca attatggcaa tatgggcttt gactaaaatt
      961 tggggtaaaa gcgatcaatg gactggggca gttggagtag cggttattat cgttatgata
     1021 ttacttagcg ttctactctt actcgctttt ccgagacaac gtcaaatcca aggttttaca
     1081 gatgcgctta atgcaactac tagagaggca ctctctggtg ttagagttat tcgtgcctac
     1141 aatgctgaaa agtatcaaac agataagttc aaacgtgaaa atgacgattt aacaaaattg
     1201 aacctttttg tttatagatt gatgtcgctt atgaatcctg tgatgacagt ggtttcatct
     1261 ggattgactt tagctattta ttggattggc tcttatttga ttaatgatat ctctgtgcct
     1321 aaagttacag gaacaagtgt atctgtattg gctgctaaat cagctatcac tgatcgagtg
     1381 gatctttttt caaacatggt tgttttttca tcttatgcta tgcaagttgt tatgggattt
     1441 atgatgatgg tagctatttt tatcatcatg cctcgtgctt tagtatcggc tagacgtatt
     1501 aatgaggtac taactttagc cccaacagta acttttaaag aaaatagtca tattcaaagt
     1561 gcacaaaaag gtcaaattga gtttcaagat gtgtcatttc attatagtaa agattctgaa
     1621 gcggttattg aacatgtatc tttcaaagca gatactggtc agacagtggc atttattggg
     1681 tctacaggtt ctggaaaatc cactttagtc aatttagttc cacgttttta tgacgccaca
     1741 gagggggcaa ttaaacttga tggcattgat ataaaggaat actctcacaa tgatttgaat
     1801 aataaaattg gctatattcc acagaaagct gttctcttta gtgggacagt acgttctaat
     1861 atgattttgg gagagagtgg cagggaggaa cttagtgatg aagaaatttg gcaagcactc
     1921 gaattagcgc aagctaaaga atttatcgag gctaaagaaa aaggattaga tacagaagtc
     1981 gctcaaggtg gtagtaactt ttcaggagga caaaaacaac gcttggcaat tgcacgtgct
     2041 ttagcacgtc accctgaaat tctcattttt gatgactcat tttcagcgtt agattataaa
     2101 acagatcata ttttacgcca tgaggtgaaa gaaaaaacag ctgctatgac gaaattaatt
     2161 gtcgctcaac gcatttcaac tatcatggat gctgatctca ttttagtgct agatcaaggt
     2221 aaagttgttg ggcaaggaac acataaggaa ttgttatcaa ataatgaagt gtatcaagag
     2281 atagcttatt ctcaattgtc taaggaggaa ttggaaaatg aaaactaaat cacaaaaaac
     2341 gtcttttttc tctaatttgt ctccgtacct caaaggttac aaacttcttt ttgcgttagc
     2401 aattgtgtta gcaactttat caagcttgac aacagttata ggaccagacc gtcttaaaga
     2461 gatgacggat cttatggtaa aaggcttggc tggtaatatt gacttagcca aaatcggtca
     2521 tattgcaggg caattggcag tattatatgt tttaggtgca ttagtatcct atctagcaag
     2581 ttttattgta acgactatta ttcaaaaatt tgctcaaggc atgcgcaatg ctattgctga
     2641 taaaatcaat aaaatacctt tgagttattt tgatagccat actcaaggtg atactttatc
     2701 tcgtgttaca aatgatgtgg atttgatgac acagtctttt aaccaaagtt tagttactat
     2761 ggttgcatct acagtattat tgattggttc attaattatg atgttcaaaa cagattggca
     2821 tttagcaaca acggcgatta tatcggtatt ttcaggattc gctctttctt caatcattat
     2881 ggtgaagagt cagcctttat ttaaaaagca acagatgaat ttggctaata tatctggata
     2941 tgtagaagaa atttactcag gacataacat tgttactagt cataacgcaa gacagcaggc
     3001 taagtcagat tttcaactat taaatgataa acttcacagt tccatgtgga aatcacaatt
     3061 tttctctgga attatgatgc ctctgatgca gtttattggt aattttggtt atgtgatggt
     3121 gtgtgtggtt ggtgccatgt tagctatcaa aggaacaatt actatgggta caattgttgc
     3181 ttttatgacc tatgttcgta tttttacaca gcctattggt cagattgctc aaggaattac
     3241 acaattacag tctgctagtg cagccatggg acgtgttttt gaatttttaa atgaaacaaa
     3301 tatgcaagag gataagcaca aagtgttaaa tcttcctaag tttaaggggt atgtgtcctt
     3361 tgaacatgtc aagtttggtt atagtgataa caagactatt attcatgact ttacttcgga
     3421 ggcacagcct ggtcagaaag ttgctattgt aggaccaact ggcgcaggta aaacgacaat
     3481 tgttaattta ttgatgcgtt tttatgatgt taattcaggt agtatttcca tagatgacat
     3541 caatatccaa gacatgaaac gtagtcaagt tcatgatgct ttctcgatgg ttcttcaaga
     3601 tatatggttg tttgagggaa ctattaaaga aaatttgatt tttaatcaga cgacaatcag
     3661 tgacgatgag gttcaagaag ctgctaaggc agtcggtgta catcatttta taatgacact
     3721 acctaaaggg tatgatattg tccttgatga cgctgtgaca ttgtcagttg gacaaaagca
     3781 attattaacc attgctcgag ctcttttgaa aaattctccc ttacttatct tagatgaagc
     3841 aacttcatct gttgatacac gtacagaaga gttaattcaa aaagctatgg acaaacttat
     3901 ggaaggaaga acatcatttg ttatcgcaca tcgtttatca acgattaaaa acgcagattt
     3961 gattttggtc atgaaagatg ggaatatcat tgagcaaggc agtcatgatg ttttgatgtc
     4021 tcaaagtggt ttttatgctg atctgtataa ttctcaattc gaatcagctt aacatgaaca
     4081 ttttagaagg aaatgggaag aaaaaattct cattcctttt tagtttgcct taagtttcta
     4141 tgatataatg gagcttaaga gagaatagga aggtttctta tggaaacaaa tatttatgga
     4201 gattatggtc gatatctacc attaatttta gaagatttca gccagcgtat acaattagaa
     4261 aatgataaag ctaaggttga aacaggctat aaattgtacg aacatatcat tggtcgtatt
     4321 aagacatcag atagtatgat agagaaatgt cgtcgtaaac agttacctgt aacagtagat
     4381 tctgcactaa aagctattag agatagtatt ggagtccgta ttatttgtgg ttttgttaac
     4441 gatatttatc aaattgtaga acgtattaag tcatttgatg attgtcgtat tgtagttgaa
     4501 aaagactata tccagcatgt taagccaaat gggtatcgct cttaccatgt gattttagaa
     4561 attgatactc cctatccaga ctgtttgggt aattcagaag gtaaatatta cattgagatt
     4621 cagttacgta caattgcgca agattcttgg gctagtttag aacatcagat gaaatacaag
     4681 catgatattg aaaatcccga acgaattgta agagaattaa aacgttgtgc tgatgaaatg
     4741 gcatctgttg atttaacgat gcaaacgatt cgtcaattga tagagagtgg aacaaagaag
     4801 gaataaaatg agagtattaa ttgcagaaga tgaagaacag atgtcacgtg tccttagtac
     4861 ggcaattagt caccaaggtt atgttgttga tgtcgcctac gatggtcaga ctgctattga
     4921 tttagctaat caaaatgcat atgatgtcat ggttatggat gtcatgatgc ctgttaaaac
     4981 aggtgttgag gctgtcaaag aaattagaca atcaggaaat aaatctcaca ttattatgct
     5041 aacagccatg gcagaaattg atgatagagt aacaggtcta gatgctggtg cagatgatta
     5101 tttaacaaaa cccttctctc taaaagaatt attagcaagg cttcgctcta tgtcccgaag
     5161 attggaagat ttcacaccta atgtattgtc attgggaaga gtaaccttat cagtcggtga
     5221 acaagagttg caatgtgaaa acactattcg tctagcgggt aaagaagcta aaatgttagc
     5281 atttttcatg ttgaaccatg ataaagaatt atctacacaa caacttttcg aacatgtctg
     5341 gggagcagat aaggaccaag aagatgtaga tgagggatac gtctttatct atgtttctta
     5401 tttgagacaa aaattgcagg caattagtgc taacttggag attattggtc aagaaggtag
     5461 tagctataaa ttaagtgaaa taaatggtgg ctaatgttta gaaatttacg acttcgtttt
     5521 atcggaatag ctgcgctagc tattcttgtt gttttatttt cagtagtcgg tgtcctcaac
     5581 tctgctaatc actatcaaac gaagaatgaa atttatcgtg tgttaactat ccttgcagac
     5641 aacaatggac gtataccgaa taaacttgaa tttagtaaag aattaggtga tgacctatca
     5701 acggacgcta ttttccaatt ccgttatttc agtgcgcgaa cagatgcaaa aggtaacgtg
     5761 actagttttg attctagaaa tatctttgaa gtaagcgata ggcaaataaa aaattatgcc
     5821 aagcgtatcg tatcacaaaa tagtgattca ggtcatatta cctataattt tagtacttat
     5881 tcttatctac taaaaaaggt tggtaaaaat gattatttag tggtattttt agatacaacc
     5941 aaccaatatc ttgataatca aagattattg caactttcaa tatggatgtc cttggcaagt
     6001 tttatcgtct tcatggtaat tgtttcagtt ttatcaggga gagttatctt accatttgtt
     6061 gctaactatg aaaagcaaag gcgttttatt accaatgcag gacatgaact aaaaactcca
     6121 cttgctatca tctccgctaa taatgaattg gtggaaatga tgagtggtga atcagagtgg
     6181 actaaatcta caaatgatca aatccaacgt ttaacgggtc taattaatgg catggtcagt
     6241 cttgcccgct ttgaagaaca gcctgatata agtatggttg acttagactt ttcacatatt
     6301 acaaaagatg ctgcagaaga ttttaaaggt ccaatcatta aagatggtaa agattttata
     6361 atgagtatcc aaccaggtat tcatgtcaaa gctgaagaaa aatcattatt tgaattggtt
     6421 acattattgg ttgataatgc taataagtat tgtgatccga tggggactgt gacagttaag
     6481 ttatcacgtt caagtcgttt gagacgagct aagcttgaag tttcaaatac ttataagaat
     6541 ggtaaagaca tagactatag caagtttttt gaacgatttt atcgagagga tgagtcacat
     6601 aataacaaaa aatcgggata tggtattggt ctttctatcg taactagttt ggtgcatcta
     6661 tttaaaggaa gtatcgatgt gaattataaa catgatacca ttacctttgt catttacatt
     6721 taagattaaa gggactgcaa ttgtagttcc tttaatttat gttattatca cgttaaagtc
     6781 gcttttttta aggatttata ttaaaatagt aaggtaatag aattgtaaag agaaaaagat
     6841 gaaagaaaag tttggtatag gaaaggctca tagcaagatt atcttgatgg gtgagcactc
     6901 agtagtatat ggttatccag ccattgctat tcctttaaaa aatattgaag taacttgcct
     6961 tattgaagag gctcctcaat taatagcatt agatatgact gatcctttgt cgacagccat
     7021 ttttgctgcc ttggactatt taggtaaaac ttcttctaaa attgcatatc atattgagtc
     7081 acaagtccca gaaagacgtg gtatgggatc atcagctgca gttgctattg cagctattcg
     7141 tgcagttttt gattattttg atgaagattt ggaggcagat ttgttagaat gccttgttaa
     7201 tcgagcagaa atgatagcac attcaaatcc gagtggatta gatgcaaaaa cttgtcttag
     7261 tgaaaatact attaaattta tccgcaatat tggtttttca acagtaccca tgcatttgaa
     7321 tgcttattta gttattgctg atacaggaat tcatgggcat actaaagaag cagtggataa
     7381 ggtgaaatca tctggcgagg cagtactacc ctttttaaaa gaattaggtt atttagcaga
     7441 ggcatcagag gatgctattc ataaaagtga ttccaaacaa cttggtagct tgatgactaa
     7501 agcgcatcaa agtttaaaac aattgggtgt ctcaagttta gaagctgatc atctggtaga
     7561 ggtagctatt agttgtggtg ctctaggcgc taaaatgagt ggcggcggat taggtggttg
     7621 tattatcgca cttgtaaaag aaaaaagaga agctgagcgc ttaagtcagc aattagaaag
     7681 agaaggagct gtcaacacat ggacggaaaa agtataagtg ttaagtctta tgctaacatt
     7741 gctattatca aatattgggg aaaagcagat gctgaaaaaa tgattccagc taccagtagt
     7801 atttctctaa cacttgaaaa catgtacact gaaacacgtt tgactaccct tgggaaggat
     7861 gctaagaaag atgaatttta catctctggt gttcttcaaa atgatcatga acatgacaaa
     7921 atgtcagcta tcttggatcg ctttcgacaa aataagtccg gctttgtaaa gattgaaaca
     7981 actaataata tgccaacagc tgcaggctta tcatcgtcat caagtggttt atcagcctta
     8041 gtaaaggctt gcaatgattt ttttggtact aaccttagcc aatcacagtt agctcaagaa
     8101 gccaagtttg cttcaggctc ttcttcacgc tctttttttg gacctgttgc tgcttgggat
     8161 aaagatagtg gtgatatcta taaagttcac actgatttag atttagctat gattatgctg
     8221 gtcttaaatg acaaaagaaa gccgatttca agccgtgaag gtatgaaaat ctgcaccgaa
     8281 acctcaacga cttttaatga atgggtaagg caatctgaac aagactatca agatatgtta
     8341 gtatacctaa aaaataatga ttttcaaaag gtaggtcaac tgacagagcg taatgcactt
     8401 gcaatgcact caacaactaa aacagcaact ccagcctttt catatttgac agaagagaca
     8461 tataaagcta tggatgttgt gaagaaactt cgcgaaaaag gtcatgaatg ttattatacg
     8521 atggatgcgg gtccaaatgt taaagtactt tgcttacgtc aagaccttga ggcattagct
     8581 gctatcttgg agaaagatta tcgtattatt gtttcaacga caaaggaatt agcagatggt
     8641 taaggtgcaa acaggtggca aattatatat tgcaggtgaa tacgcgatac tgtatcctgg
     8701 acaagtagct attttaaaga acattcctat ttatatgact gcattagcta cttttgcaga
     8761 taactacagc ctttattctg atatgtttaa ctatacggca agcttgcagc ctgataagca
     8821 atatagtctc attcaggaaa cgatattgct tatggaagaa tggttgatta attttggcaa
     8881 aaacataaaa ccgattcacc tcgaaattac gggaaaatta gagcgctatg gacttaaatt
     8941 tggtataggc tctagcggaa gtgttgtcgt tttgactatt aaagctatgg ctgcgcttta
     9001 tgagatagag atgccaagcg acttgctttt taaattatct gcctacgttc tcttaaaaag
     9061 aggtgataat ggctcaatgg gagatattgc ttgtattgct tatgagcatt taatttctta
     9121 ttcagctttt gatcgtaggg ctgtttcaaa aatgattgaa acaaaaccct tggaacaagt
     9181 attggaagct gaatggggtt atcgtattac caaaatacaa gcttcattgg agatggattt
     9241 tttagtggga tggacaatgc aaccgtccat ttcaaaagaa atgataaata tagttaaatc
     9301 aactattacg cagcgttttt tagatgatac aaattatcaa gttgtccagt tactttcagc
     9361 ctttaaagaa ggagataaag aagcgattaa gagatgttta gaagaaatta gcttgttatt
     9421 atttaatctt catccatcta tttacaccga taaacttcaa aagcttaaag aggcaagcaa
     9481 aggcctagat atagtcacaa aatcttcagg atctggagga ggcgattgtg gtatagccat
     9541 atcctttaat aaaaatgata atcaaactct aatcaagcgt tgggagagtg ctggtattga
     9601 actacttagt aaggagacat tgtcatgaca aatcgaaaag atgatcacat aaaatatgct
     9661 cttaaatatc agtcgcctta taatagtttt gacgatattg agttaataca ttcatcctta
     9721 ccaaaatata atgtcaacga tatcgattta tcaacacatt ttgctggtca aagctttgaa
     9781 tttccttttt atatcaatgc tatgacagga ggtagtgaaa aagggaaagc agtcaatcat
     9841 aaattggcac aggttgctca agctacaggg attgttatgg cgacaggttc atattcggca
     9901 gctctcaaaa acgatgaaga tgattcctac ccaacaacag acttgtatcc tgatttaaaa
     9961 ctggcgacaa atatagggtt agacaagcct ttaccagcag cagagtcaac cgttaaggct
    10021 atgaacccca tttttttaca agttcatgtt aatgtgatgc aagaactttt gatgcctgaa
    10081 ggagagcgtg aatttcacat gtggcgttct cacttaaaag aatacgtcga caatatccaa
    10141 tgcccactta tcttaaaaga ggttggtttt gggatggatt tacaatcaat aaaggatgct
    10201 tacgatatag gtatcacaac tgtagatatt tcggggcgtg gggggactag ttttgcttac
    10261 atcgaaaatc aacgtggacg cgaccgttcg tatctcaata cctgggggca aacaacagct
    10321 caatctttaa taaacgctca atctatgatg gacaaaatgg atatcctagc atctggtggc
    10381 attcgtcatc ctttagatat ggtaaaatgt ttagtgttgg gtgctaaagc tgttggactt
    10441 tctagagcag ttttagaatt agttgagcgt tatcctgtag atgatgttat tgcaattctc
    10501 aattcttgga aagaagattt aagaatgata atgtgtgcac tgaattgtaa aaaaataact
    10561 gatttacgac aagtcaatta tatattgtat ggtcaattga aggaagccaa tgctaaatga
    10621 tattattttt gtgaagtaga tatagcttcc tgtcaaagtt tccttgtaaa acgtcagagg
    10681 taataagatg attaaactgt attatgaaga tgcttacgac actgagcgat gccaagtttt
    10741 tgaaaatcca aatgatttta ttcgagtttt aatgggatgt gttactgttc ctgataatta
    10801 tcctattacg aaaatgactt ataaagataa aaatattgct tatagtggta cttatggaga
    10861 tctcttcaag acttttattg aatttgattg gtccactttg gactagtata gctaaaaagc
    10921 taaggctgtg aattgcctta gctttttagc tttatttttc ccattttacc atatttggac
    10981 ctgttggtac gatggtatga tgacgaacag ctcttccaaa tttgcctaag taatagttat
    11041 tttttatgga ttgtggattg gttaatgttt ttaaattaaa gtcattgtat aaagttttag
    11101 cccaagaaaa aatatttggg tagtcttcta gcgctttctg attaagtagt agaagatcac
    11161 gcccagcaat atcccagcgg actaatggtg taaagaaatg aatatcaacc tcacttagat
    11221 tatctccgag caagaaagga cgttgtccta gtaagtgttc tttttgatct agaaaagtga
    11281 agaattcctt actataagta tcataatctg tttgtttatc aagaaaagct aacttataca
    11341 ttaagctttg gaagttatta ttaatgtctt taatatcatt atcaataagt gaagcagttt
    11401 cttggctgaa aaggacagga gcatcctctt gatgcaaatc agagaaatct gtagtaaaaa
    11461 gtcgaagcat atctgcagat tccttacaga caatacgatt atcagtcttg tcaattaaaa
    11521 ttgggatagt ataaggacct ttgtaggtcg ggtcagtcaa atgataaata tcacgaagcg
    11581 acttagcacc agtaacctgt tctggttggc taaaaataag tcctatatca cttggaacat
    11641 catccacaaa actgatagaa atatgtttat ccaaaccaag cagttggcgt gcaatctgcg
    11701 gacgttgtgc ataaggacaa aattccccaa caatcatatg gtagcgacct gattctactg
    11761 ggacttccca ctcttggtca ataaatttag cagtaaatgt tgtcatcgta tacttccttt
    11821 caatgatatt tattgcttta agtataacaa aaaagaacct agacagatag tatttactct
    11881 aatacttatg tctaggtcct tttatattat ggtaatgagt taggaatgat aaactttaag
    11941 gctttaggga tgacttcaac ttttacaggc aattggtcac ctggatcgcc atcaatacga
    12001 actttaggga tagctgattt tttacgttta aaagttgaaa tagtcagatt attggtatga
    12061 aaatgtttta tctcctttgc ttgactaaaa tctcccttac gaaattgtct tagatgcaac
    12121 aataatttga agaagtgaat atgttccata gtgtagactc taaaaagtcc atcatcaatt
    12181 gttgcttctg gagagaaggc tggcataccg gcaatcgaat tggtcatggt aataagtagt
    12241 aaccgcgtgc gtaaagaccg tacattatta tcgtaagcaa gagtaataga atatgatcga
    12301 ttacgcttta atatacggta ggcatcacct acgaaagcaa aaggtcctaa tttacgcttc
    12361 atttcagaag tgacattagc agcaatatca gcaaggagcc caagtgttaa actgctaatc
    12421 atgtaatcgt cgttaacttt acagatatct acggacttta catgaccgtt tagaagattt
    12481 tcaagagcga gattcctctc ttgtgggatg ttcaaagctt ttgaaaagtt attgacggtc
    12541 ccggcaggta ctagtccgat agtaggataa gcaccaccag cgtagacacc accacagatt
    12601 ttattaatag tgccatctcc ccctaaagga ataaccaagt cgatatggtc ttctgatgct
    12661 ttttttgcta aggcgacagc ttcctcaaca gatgaaggtg taatgatacg aacataatcg
    12721 tcttgaaaat catgttctat taaataagat tttacttctt gtgccaatgc ttgttcatct
    12781 ttaccagatt ttggattaaa aaagatataa gca
//
LOCUS       DATRTN010000047        12714 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.047, whole genome shotgun sequence.
ACCESSION   DATRTN010000047 DATRTN010000000
VERSION     DATRTN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12714
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.047"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..974
                     /gene="fbsB"
                     /locus_tag="VBE22_001291"
     CDS             <1..974
                     /gene="fbsB"
                     /locus_tag="VBE22_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibrinogen-binding surface protein FbsB"
                     /protein_id="HEO7202756.1"
                     /translation="NTEPLTSYLENKEKFLVPNIPYKNKLILREEDKYSFEDDEEEFG
                     NELLSYNKLKNEVLPVNITTSTILKPFEQKKIVEDFNPYSNLDNLEIKKIRLNGSQKQ
                     KVEQEKTKSPTPQKETVKEQTEQKVSGNTQEVEKKSETVATSQQSSVAQTSVQQPAPV
                     QSVVQESKASQEEINAAHDAISAYKSTVNIANTAGVTTAEMTTLINTQTSNLSDVEKA
                     LGNNKVNNGAVNVLREDTARLENMIWNRAYQAIEEFNVARNTYNNQIKTETVPVDNDI
                     EAILAGSQAKISHLDNRIGARHMDQAFVASLLEVTEMSKSISSRIKE"
     gene            1041..1586
                     /locus_tag="VBE22_001292"
     CDS             1041..1586
                     /locus_tag="VBE22_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202757.1"
                     /translation="MKKITTLILASSLLLVATTSVKADDNFEMPTRYVKMSEKSKAFY
                     QRLQEKQRKAHTTVKTFNNSEIRHQLPLKQEKARNDIYNLGILISQESKGFIQRIDNA
                     YSLENVSDIVNEAQALYKRNYDLFEKIKSTRDKVQVLLASHQDNTDLKNFYAELDDMY
                     EHVYLNESRVEAINRNIQKYN"
     regulatory      1737..1826
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1982..2547
                     /gene="thiW"
                     /locus_tag="VBE22_001293"
                     /pseudo
     CDS             1982..2547
                     /gene="thiW"
                     /locus_tag="VBE22_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181415.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
     gene            2544..3098
                     /locus_tag="VBE22_001294"
     CDS             2544..3098
                     /locus_tag="VBE22_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7202758.1"
                     /translation="MKLGLKDVLLIALLAVVLGVVYFGAGYISNAFVPFVGPIAHEVI
                     YGIWFVAGPMALYILRKPGTAIVAELLAALIEVLIGSIYGPSVLVIGTLQGLGSELGF
                     TLFRYHNYKLPAFILSAILTSIFSFAWSFYANGLSAFSFSYNILMLIVRTVSSIIFFL
                     LTKNICDQLHRSGVLNAYGIHEGK"
     gene            3102..4388
                     /locus_tag="VBE22_001295"
     CDS             3102..4388
                     /locus_tag="VBE22_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7202759.1"
                     /translation="MLSVEKLAYTHGDSHYLFDEVTFSLNPGERILISGYSGCGKSTL
                     ALLLSGLKESGKGQVLLNGSLIEPSDVGFLFQNPDLQFCMDTVAHELYFILENLQIEP
                     EQMQDRSEFVLAQVGLKGFQNRLIYTLSQGEKQRLALATIFLKSPKLIILDEAFANLD
                     QESASQLLQLVLNYQANNQSMLIVIDHLITYYQDIMDHYFWLKKRLTRVDFDYMLNRL
                     NVFELEKKSHNTGDKLLSIKDFQVKLSKNKFISYLDFDLASGERLCLDGPSGVGKSSF
                     FMGLLGLYRTKGKKQFTHRKQIPISFLFQNPLDQFIFSTVYDEIFQVCKDSNKARDIL
                     ETINLWDKKQFSPFQLSQGQQRRLAIGSILASDSKLLLLDEPTYGQDAYHANMITTLL
                     LSYCHKNHCGVIFTSHDPHLKECFATRFLEVSSCSI"
     gene            4348..5040
                     /locus_tag="VBE22_001296"
     CDS             4348..5040
                     /locus_tag="VBE22_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEO7202760.1"
                     /translation="MQHVSWRCHLVQFEKLSSITFLCLLIPTFSFSFTLRLRTSLLFL
                     IIVVTLQCFVKVSLKTWAKVNLISFVMGLSLFLGTYFWGKLPHQFVLASLVACRPLIF
                     MNVGLLFHASHSNYDFIDSLYQTFKVPSHFAYGIFAVFNLLPLIKLQYQRNRLAFRLK
                     NQVTWALSPRLILSVLLKTIYWVEQLELAMLSKGFEVGKERTHALTYPVRFRDYSLLG
                     MSILLSIGMIFK"
     gene            5124..5780
                     /gene="tenA"
                     /locus_tag="VBE22_001297"
     CDS             5124..5780
                     /gene="tenA"
                     /locus_tag="VBE22_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEO7202761.1"
                     /translation="MLKDEMITKGAKYIQSIYQDPFIQGIIKGRLDHDVICHYLQADN
                     IYLGKFADIYALCLAKSDNLRDKQFFLEQIDFTLNRELADGEGPHQALAAYTNRSYQD
                     IIEKGVWYPSADHYIKHMYFHFYENGIAGALAAMSPCPWIYHQLAKKIIEENQFLNGN
                     PFNNWITFYANDTVEELMENYFRMMDYYAQNLSKEKQADLVDAFVKSCQMKDVFFKWQ
                     "
     gene            5805..6602
                     /gene="thiD"
                     /locus_tag="VBE22_001298"
     CDS             5805..6602
                     /gene="thiD"
                     /locus_tag="VBE22_001298"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO7202762.1"
                     /translation="MTYLSPVLTIAGTDPSGGAGIMADLKTFQARRTYGMAVVTSVVA
                     QNTCGVRGVQHIETAIIDQQLACVYDDIKPKAVKTGMLAERETISLVASYLKKYPQPY
                     VLDPVMVATSGHRLIDSDAVEALKEDLLPLATIITPNLPEAEILVGYDLSDEVSIIKA
                     GYDIQKQYSVRNVLIKGGHLDGLAKDYLFLEKEGLITLSNQRINTIHTHGTGCTFAAV
                     VAAELAKGQSILNAVSTAKSFITSAIETAPELGLGNGPVNHTSYQGD"
     gene            6604..7374
                     /gene="thiM"
                     /locus_tag="VBE22_001299"
     CDS             6604..7374
                     /gene="thiM"
                     /locus_tag="VBE22_001299"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEO7202763.1"
                     /translation="MYLEQLKEVNPLTICITNNVVKNFTANGLLALGASPAMSECIED
                     LEDLLKVANALLINIGTLTKESWQLYQEAIKIANKNQVPVVLDPVAAGASRFRLEVSL
                     DLLKNYSISLLRGNGSEIAALVGEKQASKGADGGKVADLESIAVKANQVFDVPVVVTG
                     ETDAIAVRGEVRLLQNGSPLMPLVTGTGCLLGAVLAAFIGSSDRSDDLACLTEAMTVY
                     NVSGEIAEKVAKGKGVGSFQVAFLDALSQMKSEMIMDK"
     gene            7388..8059
                     /locus_tag="VBE22_001300"
     CDS             7388..8059
                     /locus_tag="VBE22_001300"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001904425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEO7202764.1"
                     /translation="MKDTLKLYFVCGTVDCSRKNILTVVEEALQAGITLFQFREKGFT
                     ALQGKEKIAMAKQLQILCKQYQVPFIIDDDIDLVELIDADGLHIGQNDLPVDEARRRL
                     PDKIIGLSVSTMAEYQKSQLSVVDYIGIGPFNPTQSKADAKPAVGNRTTKAVREINQD
                     IPIVAIGGITSDFVHDIIESGADGIAVISAISKANHIVDATRQLRYEVEKALVNRQKR
                     SDVIK"
     gene            8186..9445
                     /locus_tag="VBE22_001301"
     CDS             8186..9445
                     /locus_tag="VBE22_001301"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO7202765.1"
                     /translation="MRKIIINGGKQLTGEVAVSGAKNSVVALIPATILADDIVVLDGV
                     PAISDVDSLVDIMETMGAKIKRYGETLEIDPRGVKDIPMPYGKINSLRASYYFYGSLL
                     GRYGQATVGLPGGCDLGPRPIDLHLKAFEAMGASVSYEGDSMRLATNGKPLQGANIYM
                     DTVSVGATINTMIAAAKANGRTVIENAAREPEIIDVATLLNNMGAHIRGAGTDVITIE
                     GVKSLHGTRHQVIPDRIEAGTYIAMAAAIGRGIKVTNVLYEHLESFIAKLDEMGVRMT
                     VEEDSIFVEEQEKLKAVSIKTSPYPGFATDLQQPLTPLLLTAEGNGSLLDTIYEKRVN
                     HVPELARMGANISTLGGKIVYSGPNQLSGAPVKATDLRAGAALVIAGLMAEGRTEITN
                     IEFILRGYSNIIEKLTSLGADIQLVEE"
     gene            9547..10101
                     /locus_tag="VBE22_001302"
     CDS             9547..10101
                     /locus_tag="VBE22_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7202766.1"
                     /translation="MDIWTNLGRFAFIETEHVNLRPVAYTDREAFWRIASKRTNLQFI
                     FPVQTSKKESDFLLVHSFMKEPLGVWAIEDKVSHKMFGVIRFENIDLSKKTAEIGYFL
                     KESSWGQGIMTECLKTLSFFAFQEFGMDKLIIVTHKENIASQKVALKAHFKQSRSFKG
                     SDRYTRRIRDYIEFQLTRGDYRYE"
     gene            10094..11377
                     /locus_tag="VBE22_001303"
     CDS             10094..11377
                     /locus_tag="VBE22_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO7202767.1"
                     /translation="MSKHQEILEYLENLAVGKRVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETRPRSGTVRVAQKAKVNIEKLTYAEIARISDSQVVAGTEGLSKEFSKFSIGA
                     MTHRNIEKYLVQGGLLIVGDRDEIQHLALQHQNAILVTGGFNVSPSVCRLADKLQIPV
                     MVTHYDTFTVSTMINHTLSNAKIRTDLKTVEQVYQSQMDYGFLAQDDTVKEFNLLVKQ
                     TKNVRFPIVNQANVVVGVVSVQDILGKDKEVKLATVMSKNIIVAKPRMSLANISQKMI
                     FEDLNMMPVVSDDFELLGVITRRQAVENLSMSQGTDLYTYSDQILSNLQIEDGHFSFL
                     VEPAMIDHTGSLTQGVLTEFLKEICIRVLTRKHQRSIVVKQMTLYFLQPVQIDEIIMV
                     TPTIISEKRREATLDLELKLENKIIAKAMIAVKIN"
     gene            11400..12260
                     /locus_tag="VBE22_001304"
     CDS             11400..12260
                     /locus_tag="VBE22_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEO7202768.1"
                     /translation="MITLKSAREIEAMDRAGDFLASIHIGLRDIIKPGVDMWEVEEYV
                     RRRCKEENVLPLQIGVDGAVMDYPYATCCGLNDEVAHAFPRHYTLKQGDLLKVDMVLS
                     EPLDKSIVDVSSLNFDNVAQMKKYTETYSGGLADSCWAYAVGEVSQEVKDLMSVTREA
                     MYIGIEKAVIGNRIGDIGAAIQDYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            12262..>12714
                     /locus_tag="VBE22_001305"
     CDS             12262..>12714
                     /locus_tag="VBE22_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEO7202769.1"
                     /translation="MKLKKFFEDLLAKLEYRPIQVFMRHFQSAEMDLSAIAVAYYLLV
                     TAFPLLVIAANIFPYFHINVSDLLSLMQKNLPKNIYEPASRLAVDAFSKPSTGILGFA
                     SLTAFWTMSKSLTSLQKAINKAYGVDQHRDFVISRLVGVGTGLIILFLL"
BASE COUNT         4107 a         1892 c         2632 g         4083 t
ORIGIN      
        1 tcaatacgga acctttaact agttacttag aaaataaaga aaaattttta gtccccaata
       61 ttccatataa aaataaatta attttaaggg aagaagataa atatagtttt gaagatgatg
      121 aagaagagtt tggaaatgaa cttctaagtt acaataagct taagaatgaa gttttacctg
      181 ttaatattac aacttctact atattaaaac cgtttgaaca gaagaaaatt gtggaagatt
      241 ttaatcctta ttctaattta gacaatttag aaataaaaaa aataaggttg aatggctccc
      301 aaaaacaaaa agtagaacag gaaaaaacta aatcgccaac tcctcaaaaa gagactgtga
      361 aagaacaaac tgagcaaaaa gtatctggaa atactcaaga ggtagaaaag aaatctgaaa
      421 ctgtggcaac ttcacaacaa agttcagttg cgcaaacttc tgtccaacag ccggctccgg
      481 ttcaatcagt tgttcaagaa tccaaagctt ctcaagagga gattaatgca gcacacgatg
      541 ctatttcggc gtataaatca acagtcaata ttgctaatac agccggtgta acaactgcgg
      601 aaatgaccac gctcattaat actcaaactt ctaatctttc tgatgttgag aaagctttag
      661 gaaataataa ggttaataat ggtgcagtca atgtattgag agaagataca gctcgtcttg
      721 agaatatgat ttggaatcgt gcttaccaag ctattgaaga attcaacgtc gctcgtaata
      781 cttataataa ccaaatcaag acagaaacag ttccagttga taatgatatt gaagctattt
      841 tagcaggttc tcaagctaaa attagccatt tggacaatcg tatcggagcg cgccacatgg
      901 atcaagcttt tgtagctagt ttattagaag ttactgagat gagtaaatca atctcatcgc
      961 gtataaaaga gtagacactg ctatcaaggc gatcttaaac ttttgtatta aactaaccta
     1021 aaagatagaa agagactaat atgaaaaaaa taacaacttt aatcttagct agtagcttat
     1081 tactagttgc aacgacatcg gttaaagctg atgataactt tgaaatgcca acgcgttatg
     1141 ttaaaatgag tgaaaaatca aaagcatttt atcaaagact acaagaaaaa caacgtaagg
     1201 cacatactac tgtgaagact tttaataatt cagaaataag gcatcaacta cctcttaaac
     1261 aagaaaaggc tagaaatgat atctacaatt taggcattct tatttctcag gagtctaaag
     1321 ggttcatcca acgtattgat aatgcctatt ctttggaaaa tgtctcagat attgttaatg
     1381 aagctcaggc tttgtataaa cgtaactatg atttatttga aaaaatcaaa tctacacgtg
     1441 ataaggttca agtcttactt gcatcgcatc aagataatac agacttaaaa aacttttatg
     1501 ctgagttaga tgatatgtat gaacatgttt atctcaatga aagtagagtg gaggcgataa
     1561 acagaaatat ccaaaaatat aattagtttc taaactaaca aacattccta aatataagat
     1621 attaaaccct acttattgat tagtgagtag ggttttactg ttttaaatag ctttctgctc
     1681 agaatgtaag ccttgtcatt tcaaaggaac tatgttatta ttcttaagta aattaaatag
     1741 gacatttggg gtgcgtaaca gctgagatta tacccattga acttgataca gttagtactg
     1801 tcgaagggaa atgtgtgcca ttacaagttt tttgtggtgg aaaagggcgt agtgtcttct
     1861 taggagacag ctagctctag ttaatcagat aataaactcc gtttgcttgc agatggagtt
     1921 ttttgttgtc aaaatttttt gtaggttgtt gccccttatc tgtcatagag aaggagttat
     1981 gatgactaat cataaaacaa aaaattaacg gttttagcaa ttatgattgc tttagatgtt
     2041 tttctaacgc ccatttttag aatagaaggc atggccttta tgtctagtgt ggttaatatt
     2101 atcgctggag ttataatggg accaatctat gcattagtta tggcacttgt aacggctttt
     2161 attcgtattt taactcaagc aggagtagca tctgttgcac ccttagctat tctaggagct
     2221 ggtccaggag ctttcttagc gggttggctt tatcagtttt atgcgaaaga ctggatgtct
     2281 tgggtgggtg agtttatagg aacagggata attggagcac ttttatcagc tccaattatg
     2341 gtctggtttt ggacttggac agcggatgga aatcacgaag tgttagctaa agcatctgca
     2401 gctcaatcca tttttctttt tatgccaagg tttatagggg cgactataat tggcggtgtt
     2461 attgctttgt tgctacttca gggattaaag cgggttcctg tatttatgac aatacaagca
     2521 ttatttaaag gagaaaaagt aaaatgaaat tgggtttaaa agatgtatta ttgattgctt
     2581 tattagcggt tgttctgggc gttgtttact ttggagcagg ctatatttca aatgcttttg
     2641 taccttttgt aggtcctata gcgcatgaag ttatttatgg tatttggttt gtggcaggtc
     2701 caatggctct ttatatattg aggaaaccag gaaccgctat tgtagcagaa ttgttagctg
     2761 ctttgataga ggttttaatt ggatcaattt atggaccaag tgtactcgtt attggtaccc
     2821 ttcaaggttt agggtcagaa ttaggcttta cgctgtttag gtatcataat tacaagttgc
     2881 cagcttttat attgtctgct attttgacat caatttttag ttttgcatgg agtttttatg
     2941 ccaatggact tagtgcattt tcattttcat ataacattct gatgcttata gtaagaactg
     3001 tttcatctat tattttcttc ttattaacaa agaatatatg tgaccaattg catagaagtg
     3061 gtgtactgaa tgcttatggg attcatgaag ggaagtagcc tatgctatct gttgaaaaat
     3121 tagcttacac ccatggcgat agtcattatt tatttgatga agtgactttt agtcttaacc
     3181 caggagaacg aattttaatt agtggctatt cagggtgtgg taagagtaca ttggctcttt
     3241 tactatcagg gttaaaagaa agtgggaaag gtcaagtttt attaaacggc tcattaatag
     3301 aaccaagcga tgtaggtttt ctttttcaaa atcctgattt acaattttgt atggatactg
     3361 tggcgcatga gttgtatttt attttagaaa atttacaaat agaaccggag caaatgcaag
     3421 atagaagtga gtttgtattg gcacaagttg gtttaaaagg atttcagaat cggttgattt
     3481 atactttatc tcaaggtgaa aaacagcgat tagcattggc gaccattttt ttaaaatctc
     3541 ctaaacttat tattttagat gaagcttttg cgaatttgga tcaagagagt gccagtcaac
     3601 tattacaatt agttcttaat tatcaagcaa acaatcaatc aatgctgatt gtaatcgatc
     3661 atttgattac ttattatcaa gatataatgg atcactattt ttggttgaaa aaacggttga
     3721 caagggtcga ttttgattat atgctcaatc gtttgaatgt cttcgaatta gaaaagaaaa
     3781 gtcataatac aggtgacaaa ctgttatcaa ttaaagattt ccaagttaaa ctttcaaaaa
     3841 acaaattcat ctcttacctt gattttgatt tagcaagtgg ggagaggttg tgcttagatg
     3901 gaccaagtgg cgtggggaag tctagtttct ttatgggatt gttagggctt tataggacta
     3961 aagggaaaaa acaatttact catagaaagc aaattcctat aagcttttta tttcaaaatc
     4021 ccttagacca atttattttt tcaacagttt atgacgaaat ttttcaggtt tgcaaggata
     4081 gtaacaaagc gagggatatt ttggaaacca taaatctttg ggataagaaa cagttttcac
     4141 catttcaact aagtcaaggg caacagaggc gattagctat tggcagtatt ttagctagtg
     4201 atagtaaact tctattatta gatgaaccaa cttatggaca agatgcctat cacgctaata
     4261 tgattacgac tcttcttttg agttattgtc acaaaaatca ttgtggggtt atttttacta
     4321 gccatgatcc tcacttaaaa gagtgttttg caacacgttt cttggaggtg tcatcttgtt
     4381 caatttgaaa aactatcatc tattaccttc ctatgtttat taataccaac cttttcgttt
     4441 agtttcacat tgaggttaag aacgtctttg ttatttctga ttatagtagt aacactgcaa
     4501 tgcttcgtaa aggtttcttt aaaaacttgg gcgaaggtca atctgatctc atttgtgatg
     4561 ggactctctt tatttctagg gacgtatttt tgggggaaat taccccatca attcgtctta
     4621 gctagtctag tagcctgtcg tccgcttatt ttcatgaacg tgggtttgtt atttcatgca
     4681 tcacattcta attatgattt tatagactct ctttatcaaa ctttcaaagt gcctagtcat
     4741 tttgcatatg gtatttttgc ggtctttaat cttctaccat taattaaatt acaataccaa
     4801 cgtaatcgac tcgcattccg actgaagaat caggtaactt gggcattatc gcctagacta
     4861 atattgtcag ttttattaaa gacgatatat tgggtagaac agttagagtt agcaatgctt
     4921 tctaaaggtt ttgaagtggg gaaagagcga acccatgctt taacttatcc cgttcgtttt
     4981 cgagattata gtttacttgg tatgagtatt ttattgtcta taggaatgat ttttaaataa
     5041 gtacaattag tttagtaata caattgtgca tttatataga gtgagcgtgt tctgctcatg
     5101 agatcataac aaaggagatt actatgttaa aagatgagat gattactaaa ggagcaaaat
     5161 atatccaatc catctatcaa gatccattta ttcaaggaat tataaaaggg cgtttagacc
     5221 atgacgtaat ctgccattat ttacaggcag ataatatcta ccttgggaaa tttgctgata
     5281 tttatgccct gtgcttggca aaatctgata atttacgaga taaacaattt ttccttgaac
     5341 aaattgactt tacattaaat cgggagttag cagatggtga aggcccacat caagccttag
     5401 ccgcttatac taatcgatct tatcaggata ttatcgaaaa gggtgtatgg tacccgtcag
     5461 cagatcatta cattaaacat atgtattttc atttttacga aaatggtatt gctggtgctt
     5521 tagctgcaat gagcccatgt ccatggattt accatcaatt ggctaaaaaa ataattgagg
     5581 agaaccagtt tttgaatggt aaccctttta ataattggat aactttttat gctaacgata
     5641 cagttgaaga gttaatggaa aactattttc gtatgatgga ttattatgcg cagaatttgt
     5701 caaaagagaa gcaagctgat ttggtagatg cttttgtgaa atcttgtcag atgaaagacg
     5761 tttttttcaa atggcaataa atcaagaaaa atgggaggat taatatgact tatttatcac
     5821 cagttttaac tattgcagga actgatccta gcggtggagc agggattatg gcagatttaa
     5881 agacatttca agctcgtcgg acttacggga tggctgttgt gactagtgtt gttgctcaga
     5941 acacttgtgg ggttagaggc gttcagcata tagaaacagc tatcattgat caacaactag
     6001 cttgtgttta tgatgatatt aagccaaagg cggtcaaaac aggaatgtta gcggagaggg
     6061 aaacgattag tttagtcgct tcatatttaa aaaaatatcc gcaaccttat gtattagatc
     6121 ctgttatggt ggcaactagc ggacatcgtt taattgattc agatgccgta gaagcattaa
     6181 aagaagactt attgccatta gcaactatta tcacaccaaa tttaccagaa gctgagattt
     6241 tagtgggata tgacttatca gatgaagtaa gtattattaa agcaggttac gatattcaaa
     6301 aacaatattc tgttagaaat gttcttataa agggaggaca tctagatggt ttggctaaag
     6361 attatttatt tttggaaaaa gaagggttga taactcttag taatcaacgt atcaatacta
     6421 ttcatactca tgggactggg tgtacatttg cagcagtcgt tgcagcggag ttagctaaag
     6481 ggcaatcaat tctaaacgcg gtaagtactg ctaaatcatt tattacttca gctatcgaaa
     6541 cagcgcctga gttgggtctt ggaaacggtc ctgtcaatca tactagctat caaggagact
     6601 aaaatgtatt tagaacaatt aaaagaggta aatcctttaa ccatttgtat cactaataat
     6661 gtggtcaaaa actttacagc taatggttta ttagctttag gagcttctcc ggctatgagc
     6721 gagtgcatag aggacttaga agacctgtta aaagttgcaa atgcattatt aattaatata
     6781 ggaacactaa caaaagaaag ttggcaactt taccaagaag caatcaaaat agcaaataaa
     6841 aaccaagttc cagttgtatt agatcctgta gcggcaggag caagtcgttt tcgattagaa
     6901 gtgtcacttg atttattgaa gaattattct attagtttac ttagaggaaa tggtagtgaa
     6961 atcgctgctt tggttggtga aaaacaagcg tctaaagggg cagatggggg aaaagtggct
     7021 gatttagaat caattgctgt aaaagccaat caagtttttg atgtgccagt ggttgtcaca
     7081 ggagaaactg atgctatcgc tgttagagga gaagttcgct tattacaaaa tggtagtcct
     7141 ttaatgccac ttgttacagg aactggatgt ctattagggg cagtattggc tgcttttatt
     7201 ggtagttctg acagaagcga tgatcttgct tgtctgacag aggccatgac tgtttataat
     7261 gtttctggag aaattgcaga gaaagtggca aaaggtaagg gagtgggaag tttccaagtt
     7321 gcatttttag atgccctttc ccagatgaaa tcagaaatga ttatggacaa ataggtggtg
     7381 tcagtagatg aaagatacat tgaaattata ttttgtttgt gggactgtgg attgctctag
     7441 gaagaatata ttgactgttg ttgaagaagc cttacaagca ggtataactc tatttcaatt
     7501 tagagaaaaa ggttttacag cattacaagg taaggaaaaa attgctatgg ctaagcaact
     7561 ccaaatactt tgtaagcaat atcaggttcc ttttattatt gatgatgaca ttgacttagt
     7621 tgagttaatt gatgctgatg gtcttcatat tggacaaaat gatttacctg ttgatgaggc
     7681 acggagacgt ttaccagata aaatcatcgg tttatctgtg agtacaatgg ctgaatatca
     7741 gaaatcacaa ttatctgttg ttgattacat cggaattggg ccttttaatc cgacacaatc
     7801 aaaagcagat gctaagcctg ctgttggcaa taggacaaca aaggcagtta gagaaattaa
     7861 tcaagatatt ccaatcgttg caataggcgg aatcactagt gattttgttc atgatattat
     7921 agaatcaggt gcagatggga ttgctgttat atcggctatt tcaaaagcaa atcatattgt
     7981 agatgcaacc agacaattgc gctatgaagt tgaaaaagct ctggttaata ggcaaaaacg
     8041 cagtgatgta atcaaataag gctatatcgt taattataag aatttttaag atattatatc
     8101 atcacacaat actggtattt gaaaaagaat ttaaactatg ataatatagt agagttgaat
     8161 tctagttaaa gaaagatttg aaagtatgcg taaaattatt attaacggtg gcaagcaact
     8221 gacgggagaa gttgctgtat cgggtgctaa aaatagtgta gttgccttga ttcctgctac
     8281 tatacttgct gatgatattg tggtcttaga tggagttcca gctatttctg atgttgacag
     8341 tttagttgac attatggaaa ctatgggagc taagattaag cgttatggtg agacgcttga
     8401 aattgatcca cgtggcgtta aagatattcc aatgccttat ggcaaaatca atagtcttcg
     8461 tgcatcctat tatttctatg gaagcttatt ggggcgttat ggtcaagcaa cagtaggttt
     8521 acctggtggt tgtgatttag gcccccgtcc gattgattta catttaaaag cttttgaagc
     8581 gatgggagct agtgtttctt atgaaggtga ttcaatgcgt ctagcaacaa acggaaagcc
     8641 tttgcaagga gcaaatattt atatggatac tgtcagtgtt ggtgcgacaa ttaacactat
     8701 gatcgcagca gctaaggcaa atggtcgtac ggttattgaa aatgctgcgc gtgaaccaga
     8761 aattattgac gtggctactc ttttaaataa tatgggagct catattcgtg gtgctgggac
     8821 tgatgttatt acaattgaag gtgtgaaatc gctccatggc acgcgtcatc aagtaattcc
     8881 agatcgtatt gaagcaggaa cttatattgc tatggcagca gctatcggac gaggaataaa
     8941 agtgaccaat gtgttatatg aacaccttga gagtttcatt gctaagttag atgagatggg
     9001 agttcgaatg acagttgaag aggattcaat ttttgtcgaa gaacaagaaa agttaaaagc
     9061 tgtatccata aaaacctcac cttacccagg atttgctaca gatttacaac aacctctaac
     9121 ccctttatta ctaacagcag aaggaaacgg gagtttatta gatactatct atgagaaacg
     9181 tgttaatcat gttcccgaat tagcacgtat gggagctaat atttctaccc tagggggcaa
     9241 aatagtttat agtggtccta atcaattatc tggtgcacct gtaaaagcga cagatttgcg
     9301 tgcaggcgca gctttagtta tcgctggctt aatggcagag ggacgaactg aaattacgaa
     9361 catcgagttt attttacgtg gatattctaa tatcatcgag aaactaacat cattgggtgc
     9421 ggatatccaa ttggttgagg aatagtatat ataacaaaga gttgagatta tttcttagct
     9481 cttttcttct tgttttagat taagtcactt ataggtattt gaaatctctt gtggtataat
     9541 attatcatgg atatatggac aaatttagga cggtttgcat tcattgaaac agaacatgtt
     9601 aacctacgtc ctgttgccta cactgatagg gaagcttttt ggcgaatagc ttcaaaaagg
     9661 acaaatttac aatttatttt tcctgttcaa accagtaaga aggaaagtga ttttctttta
     9721 gttcattctt ttatgaaaga gccgttggga gtttgggcga ttgaagataa agttagtcat
     9781 aaaatgtttg gagttatccg ttttgaaaac attgatttat ctaaaaagac agctgaaatt
     9841 ggctattttt tgaaagaaag ttcttggggt caaggcatta tgactgaatg tctaaagaca
     9901 ctttcttttt ttgcttttca agagtttggg atggacaaat tgatcatagt aacacataaa
     9961 gagaatattg ctagtcagaa ggttgctttg aaggcgcatt tcaaacaatc taggagtttt
    10021 aaaggaagcg accgttatac acgccgtata agagactata ttgagtttca attaactagg
    10081 ggtgattatc gttatgagta aacatcaaga aattttggag tacctagaaa atttagctgt
    10141 tggtaagagg gttagtgtac gcagtatttc aaatcattta aaggtgagtg atggcactgc
    10201 ataccgcgct ataaaagagg cagaaaatag ggggattgtt gagactagac cgcgtagtgg
    10261 gacagtacgt gttgctcaaa aagcaaaggt taatattgaa aaactgactt atgctgaaat
    10321 tgcaaggatt agtgactcac aagttgttgc aggaacagag ggactcagca aggagtttag
    10381 taaattttct ataggtgcaa tgactcacag aaatattgaa aaatatttgg tgcaaggagg
    10441 gttattgatt gttggcgacc gtgatgaaat acaacattta gccttacaac atcaaaacgc
    10501 tatcttagtt acaggtggct ttaatgtttc cccatctgtt tgtcgtttag cagataaact
    10561 tcaaattccc gtaatggtaa cacactatga tacattcaca gtatcaacga tgattaatca
    10621 cactttgtca aatgccaaaa ttcgtactga tttaaagacc gttgaacaag tctatcagtc
    10681 gcaaatggat tacggtttct tagcgcagga tgataccgta aaagaattta atcttcttgt
    10741 taaacaaact aaaaatgttc gttttccaat agtcaaccaa gctaatgttg tcgttggtgt
    10801 tgttagtgtt caagatattc ttggaaaaga taaagaagta aaattggcaa cagtcatgtc
    10861 aaaaaatatc attgttgcta aacctcgaat gagtctagct aatatcagtc aaaaaatgat
    10921 ttttgaagat ttgaatatga tgccagtagt atcggatgac tttgaattat taggcgttat
    10981 tacaagacga caagctgtag aaaatttatc gatgtcgcaa gggacagatt tatacaccta
    11041 tagtgatcaa atcttgtcta atttacaaat tgaagatggt cacttttcgt ttttagtaga
    11101 acccgctatg attgaccata caggtagctt aacacaagga gttttgacgg agttcttaaa
    11161 agaaatttgt attcgtgtct taactcgtaa gcatcaacgc agtattgttg ttaaacaaat
    11221 gacactttat ttcttgcaac ctgttcaaat tgatgaaata ataatggtaa cgccaacaat
    11281 tatttcagaa aaacggcgcg aagctacact cgatttagaa ttaaaattag aaaataaaat
    11341 tattgctaaa gctatgatag cggtaaaaat taattagata taaggagaat aaatagaaaa
    11401 tgattacatt aaagtcagca cgcgaaattg aagcaatgga tagagcaggt gattttttag
    11461 ccagcatcca cataggactt cgtgatatta tcaaaccagg agttgatatg tgggaagttg
    11521 aagaatacgt tcgtagacgt tgtaaagaag aaaacgtatt gccattgcaa atcggggttg
    11581 atggtgcagt tatggattac ccatatgcta cttgttgtgg gcttaatgat gaagtggctc
    11641 atgcctttcc tcgtcattat accctaaaac aaggtgacct tttaaaagta gatatggttt
    11701 taagcgaacc tttagataaa tcaattgttg atgtttcatc gttaaacttt gacaacgtag
    11761 ctcaaatgaa aaagtacacg gaaacatata gtggtggttt ggcagactcg tgttgggctt
    11821 atgctgtagg tgaagtttca caagaggtga aagacttaat gtctgtgaca agagaagcaa
    11881 tgtatattgg gattgaaaaa gcagttatcg gaaaccgtat tggtgatatt ggagctgcta
    11941 ttcaggatta tgcagagagt cgtggttatg gagttgttcg tgatttggtt ggtcacggtg
    12001 ttggtccaac gatgcatgaa gagcctatgg tgcctaacta tggtacagca ggtcgaggtc
    12061 tccgtcttcg tgaggggatg gttttaacca tcgaaccaat gattaatact ggtacttggg
    12121 aaattgatac agacatgaaa actggttggg cacataaaac cttagatggt ggtctttctt
    12181 gtcaatatga acatcaattt gttatcacta aagatggtcc tgttatttta acgagtcaag
    12241 gagaagaaag aacttactaa aatgaaatta aaaaaattct ttgaggattt gctggctaaa
    12301 ctagaatata gacccattca agtttttatg cgtcattttc aaagtgcaga gatggactta
    12361 tcagccatag cagttgctta ttatctatta gtgacagctt ttccattact agttattgct
    12421 gctaatattt ttccttattt tcatattaat gtgtcggatt tattgtcact tatgcaaaaa
    12481 aatcttccga aaaatattta tgaacctgcc tcacgcttag cagtagatgc tttttcgaaa
    12541 ccgtctaccg gcattttggg attcgcttca ttaacagctt tttggacaat gtctaaaagt
    12601 ttgacctctc ttcaaaaagc cattaataaa gcatacggtg ttgaccaaca cagagacttt
    12661 gtgattagtc gtttagtggg tgtggggact gggctgatca tcctattttt actg
//
LOCUS       DATRTN010000048        12552 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.048, whole genome shotgun sequence.
ACCESSION   DATRTN010000048 DATRTN010000000
VERSION     DATRTN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12552
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.048"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            245..316
                     /locus_tag="VBE22_001306"
     tRNA            245..316
                     /locus_tag="VBE22_001306"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:277..279,aa:Gln,seq:ttg)
     gene            325..396
                     /locus_tag="VBE22_001307"
     tRNA            325..396
                     /locus_tag="VBE22_001307"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:358..360,aa:Glu,seq:ttc)
     gene            459..977
                     /gene="rimM"
                     /locus_tag="VBE22_001308"
     CDS             459..977
                     /gene="rimM"
                     /locus_tag="VBE22_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEO7202770.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVTDFVEERFKKGQELALFDEK
                     NQFVMDIEIASHRKQKNFDIIKFKGMYHINDIEKYKGFTLKVAENQLSDLKDGEFYYH
                     EIIGLDVYEGEELIGKIKEILQPGANDVWVVERHGKRDLLLPYIPPVVLEVDLSNQRV
                     QVELMEGLDDED"
     gene            964..1719
                     /gene="trmD"
                     /locus_tag="VBE22_001309"
     CDS             964..1719
                     /gene="trmD"
                     /locus_tag="VBE22_001309"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEO7202771.1"
                     /translation="MMKIDILTLFPEMFAPLEHSIVGKAKERGLLEINYHNFRENAEK
                     SRHVDDEPYGGGQGMLLRAQPIFDTIGKIDAQKARVILLDPAGRTFDQNFAEELSKED
                     ELIFICGHYEGYDERIKSLVTDEVSLGDFVLTGGELAAMTMVDATVRLIPEVIGKETS
                     HQDDSFSSGLLEYPQYTRPYDYLGMTVPDVLMSGHHENIRKWRLEQSLRKTLERRPDL
                     LENYAMTDEERLILEKIKTEIERTDTVNEQNNL"
     gene            1700..2692
                     /locus_tag="VBE22_001310"
     CDS             1700..2692
                     /locus_tag="VBE22_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO7202772.1"
                     /translation="MNKTIYDITIVGGGPVGLFAAFYAGLRGVSVKIIESLSELGGQP
                     AILYPEKKIYDIPGYPVITGRELIDKHIEQLERFKDSIEICLKEEVLSFEKVDDVFTI
                     QTDKDQHLSRAIVFACGNGAFAPRLLGLENEENYADNNLFYNVTKLEQFAGKHVVICG
                     GGDSAVDWANELDKIAASVAIVHRRDAFRAHEHSVDILKASGVRILTPYVPIGLNGDS
                     QRVSSLVVQKVKGDEVIELPLDNLIVSFGFSTSNKNLRYWNLDYKRSSINVSSLFETT
                     QEGVYAIGDAANYPGKVELIATGYGEAPVAINQAINYIYPDRDNRVVHSTSLIK"
     gene            2829..3899
                     /locus_tag="VBE22_001311"
     CDS             2829..3899
                     /locus_tag="VBE22_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO7202773.1"
                     /translation="MTNTVTPKETAGSFINKVLGGTATAIVVALIPNAILATFLKPFL
                     SYGLAAEFLHIVQVFQFFTPIMAGFLIGQQFKFTPMQQLAVGGAAYIGSGAWAYTEVI
                     QKGVATGSFQLRGIGDLINMMLTAALAVLAVKWFGNKFGSLTIILLPIIIGTGVGYLG
                     WKLLPYVSYVTTLIGQGINSFTTLQPIAMSILIAMAFSMLIVSPISTVAIGLAIGLNG
                     MSASAASMGVASTTAVLVWATMKANKSGVPIAIALGAMKMMMPNFLKHPVMAIPMLMT
                     ATVSSLTVPLFKLVGTPASSGFGLVGAVGPIASFEAGASMLIVILSWLVIPFAVGFVS
                     HKICKDILKLYKDDIFVFEGQN"
     gene            3912..4835
                     /locus_tag="VBE22_001312"
     CDS             3912..4835
                     /locus_tag="VBE22_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEO7202774.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNHDVILLDNWADHIMAIKENGL
                     KVTGDTEDLVKLPIMKPTDATEEADLIILFTKAMQLPNMLQDIKKIIGKKTKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLKGPGHAHLEGVGSVNLQSIDPNNQEAGHRV
                     TELLNEAKLQATYDENVLPNIWRKACVNGTMNSTCALLDCTIGQLFASEDGVNMVHEI
                     IHEFVTVGKAEGVELDEEEITKYVMDTSVKAAHHYPSMHQDLVQNQRLTEIDFLNGAV
                     NKKGENLGIDTPYCRLITQLIHTKENVLSIK"
     gene            5047..6681
                     /locus_tag="VBE22_001313"
     CDS             5047..6681
                     /locus_tag="VBE22_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEO7202775.1"
                     /translation="MMKKGQVNDTKQSYSLRKYKFGLASVILGSFIMVTSPVFADQTT
                     SVQVNNQTGTSVDANNSSNETSASSVITSNNDSVQASDKVVNSQNTATKDITTPLVET
                     KPMVEKTLPEQGNYVYSKETEVKNTPSKSAPVAFYAKKGDKVFYDQVFNKDNVKWISY
                     KSFCGVRRYAAIESLDPSGGSETKAPTPVTNSGSNNQEKIATQGNYTFSHKVEVKNEA
                     KVASPTQFTLDKGDRIFYDQILTIEGNQWLSYKSFNGVRRFVLLGKASSVEKTEDKEK
                     VSPQPQARITKTGRLTISNETTTGFDILITNIKDDNGIAAVKVPVWTEQGGQDDIKWY
                     TAVTTGDGNYKVAVSFADHKNEKGLYNIHLYYQEASGTLVGVTGTKVTVAGTNSSQEP
                     IENGLAKTGVYNIIGSTEVKNEAKISSQTQFTLEKGDKINYDQVLTADGYQWISYKSY
                     SGVRRYIPVKKLTTSSEKAKDEATKPTSYPNLPKTGTYTFTKTVDVKSQPKVSSPVEF
                     NFQKGEKIHYDQVLVVDGHQWISYKSYSGIRRYIEI"
     gene            6825..8060
                     /locus_tag="VBE22_001314"
     CDS             6825..8060
                     /locus_tag="VBE22_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO7202776.1"
                     /translation="MTLRELTIEEFKEHSGNYDSQSFLQTPEMAKLLEKRGYDVRYLG
                     YRVENKLEIISLSYIMPVTGGFQMKIDSGPVHSNSKYLKQFYKALQGYAKSNGVLELI
                     VEPYDDYQLFTSSGVPSNQGNDNLIEDFTSSGYHHDGLTTGFTGKYLSWHYVKNLEGV
                     TSETLLSSFSKTGRALVKKAMSFGIKVRVLKRDELHLFKEITTSTSNRRDYMDKSLDY
                     YQDFYDSFEGKAEFVIATLNFREYDHNLQIKAEALENKLKLLDERFRENADSPKYHRQ
                     RSEIINQLASFETRRQEVQSFIQKYDNQDVVLAGSLFVYSLKETVYFFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRGSTFYNLLGIQGTFDGSDSILRFKQNFNGYIIRKMGTFNYY
                     PSPFKYKGIQLLKKVLKRN"
     gene            8125..8934
                     /locus_tag="VBE22_001315"
     CDS             8125..8934
                     /locus_tag="VBE22_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO7202777.1"
                     /translation="MLDIERKRDIIKLQTISIQGGTMLKSKRKEIILSRLEQNKSVTL
                     DELTSILETSESTVRRDLDELESEGFLKRVHGGAELPYSLGQELSNQEKAIKNVQKKL
                     DIARQTAKLIAKQDVIFIDAGTTTELLIDFLPHEQLTVVTNSIHHAAKLVDRGIKTII
                     IGGAVKHSTDASIGQVAINQIRQITVDKAFLGMNGIDEVYLTTPDLEEAAIKEAIINN
                     SQQTFILMDSSKIGQVTFAKVKEINDINLVTNKTDSELMTIIKEKMKVIQV"
     gene            8931..9842
                     /gene="pfkB"
                     /locus_tag="VBE22_001316"
     CDS             8931..9842
                     /gene="pfkB"
                     /locus_tag="VBE22_001316"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEO7202778.1"
                     /translation="MIYTVTLNPSIDFIVRLDTLLLGSVNRMTSDDKYVGGKGINVSR
                     ILKRLKIDNTATGFIGGFTGHFVEDGLVSEGIKTDFVSVNEDTRINVKVKAKIETEIN
                     GGGPRITNEQLHRLEKLLSRLTPEDTVVFAGSAPASLGNKVYNTLIPIAKKTGAEVVC
                     DFEGQTLLDALAYQPLLVKPNNHELADIFGVELEGLPDIEKYAHKILDKGAKNVIVSM
                     AGDGALLVTPEASYFAKPIKGEVKNSVGAGDSMVAGFTGEFVKSKNPVEALKWGVACG
                     TATTFSDDLATAEFIQDIYNKVEVEKL"
     gene            9839..11803
                     /locus_tag="VBE22_001317"
     CDS             9839..11803
                     /locus_tag="VBE22_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7202779.1"
                     /translation="MKIQDLLKKEVMIMDLKATSKEAAIDEMITKLVDTGVVTNFAIF
                     KDGIMKREAQTSTGLGDGIAMPHSKNAAVKEATVLFAKSASGVDYEALDGQPTDLFFM
                     IAAPDGANDTHLAALAELSKYLLKEGFADQLRQAKTPDDIIATFDSNSISQETVAPQT
                     VQSTSKGSDYIVAVTACTTGIAHTYMAEEALKKKAAEMGVGIKVETNGASGVGNKLTS
                     SDIARAKGVIIAADKAVEMDRFDGKPLVSRPVADGIKKSEDLINIILDNKAQTYHAKN
                     QNDKQSGESDGKSGLGSAFYKHLMGGVSQMLPFVIGGGIMIAIAFLFDNILGVPKDQL
                     SNLGSYHEIAALFKNIGGAAFAFMLPVLAGYIAYSIAEKPGLVAGFVAGSIASSGLAF
                     GKVPFAEGGKATLALAGVPSGFLGALVGGFLAGGVILLLRKLLSGLPKSLEGIKSILL
                     YPLLGVLITGFLMLLVNIPMAAINTALNTFLQGLSGSSAVLMGLLVGGMMAVDMGGPV
                     NKAAYVFGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFNNEERQSGL
                     TNIVMGLSFITEGAIPFGAADPARAIPSFIVGSALTGALVGLAGIKLMAPHGGIFVIA
                     LTSNPLLYILFILIGAVVSGVLFGLFRKTK"
     gene            11992..12486
                     /locus_tag="VBE22_001318"
     CDS             11992..12486
                     /locus_tag="VBE22_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEO7202780.1"
                     /translation="MIKWNEKETKLVDTSFSIMLYLKTLFAVGQVVAGVLLYYFKPTQ
                     IKHLLFLLTEGEVHGGIHGFISAHFLKISSYINDSASHLAAIYLLIHGGIKLVALILL
                     HKEKLFAYPLSILVFVGFIIFQLREYFSSHSILMIFLCVFDVIMIILTWLEYQKMKLN
                     FSKR"
BASE COUNT         4183 a         1855 c         2539 g         3975 t
ORIGIN      
        1 gtgtggctat acaagatata ttagaaattg ggatgaataa taagcagtac catgaactac
       61 ttttacaagc tattatcgaa aaaagggacc aaaaagtttt tgaatatatt tcaaaaatga
      121 aacataaaat actttgatag actctatatg agctttatta aaagggataa aatcgaaatt
      181 taaaatttaa ttcgaaaaga tattgacagt aatctagaat ttcgatatac tagattttgt
      241 cttttggggt atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg
      301 aatccagcta ccccagtttc acatggtccg ttggtcaagg ggttaagaca ccgccttttc
      361 acggcggtaa cacgggttcg aatcccgtac ggactatcgt ggccagttgc attttagcgg
      421 ctggtttttc ttttattttc aaaaatatcg gagttactat ggaatacttt aacgttggaa
      481 aaattgtcaa tacacaagga ttacaaggcg aaatgcgtgt attaagcgtg actgactttg
      541 tagaagaacg ctttaaaaaa ggtcaagagt tagctttatt cgatgaaaag aatcagtttg
      601 ttatggatat tgaaattgct agtcatcgta agcagaagaa ttttgacatt ataaaattca
      661 aaggaatgta tcatattaat gatattgaaa aatataaagg tttcacactg aaggtggcag
      721 agaatcaact gagcgacctt aaagatggtg aattctatta tcatgaaatt attggcttag
      781 atgtctatga aggggaagag ctgattggaa aaatcaaaga aattttacaa ccaggagcaa
      841 atgacgtttg ggttgtggaa cgtcatggaa aacgtgatct tttattaccc tatatcccgc
      901 cagtagtatt agaagtggac ctttctaatc agcgtgttca agttgaattg atggaaggtt
      961 tagatgatga agattgatat tttaacttta tttcctgaaa tgtttgcccc tctggaacat
     1021 tctatagttg gtaaggcaaa agaaagagga cttcttgaaa ttaattatca caatttcaga
     1081 gaaaatgctg agaagtctcg acatgttgat gatgagcctt acggtggggg gcaagggatg
     1141 cttttacgtg ctcaacctat ttttgataca attggtaaaa ttgatgctca aaaagcacgt
     1201 gttattttgc tagacccagc gggcagaaca tttgatcaga attttgcaga agaactatcc
     1261 aaagaagatg aattaatttt tatttgtggt cactatgaag gttatgatga acgtataaaa
     1321 tcattagtaa cagacgaagt atctttggga gattttgttc ttacgggagg agaattggcg
     1381 gctatgacaa tggttgatgc taccgttcgt ctgattccag aagttatagg taaagaaaca
     1441 agtcatcagg atgattcatt ttcatcgggt ctattagaat atcctcagta tacgcgccca
     1501 tatgattatt taggcatgac agttcctgat gttctcatga gtggtcatca tgaaaatatc
     1561 cgaaaatggc gtttagagca aagtttgcga aaaacactag aacgtcgacc agacttgtta
     1621 gaaaattatg caatgacgga tgaagagaga cttatcttag agaaaattaa aacagaaata
     1681 gaaagaacag atacagtaaa tgaacaaaac aatttatgat atcactattg taggaggcgg
     1741 accagtaggt ctctttgctg ctttttatgc tggactacgc ggagtatccg ttaaaattat
     1801 tgaaagcctt tcggaattag gaggccagcc agctatcctt tatcctgaga aaaaaatcta
     1861 tgatattcct ggttatccgg tgattactgg acgtgaatta attgataaac atattgaaca
     1921 attagagaga tttaaagatt ctattgaaat ctgtttaaaa gaagaagttt tatcatttga
     1981 aaaagtagat gatgttttca caattcaaac agataaagat caacatttgt cacgtgctat
     2041 tgtttttgct tgtggcaacg gtgcttttgc tccgcgttta ctaggtttgg aaaatgaaga
     2101 aaattatgca gataataatt tattctataa cgttacaaag ttagaacagt ttgcaggtaa
     2161 acatgttgtt atttgtggtg gaggcgactc agctgttgat tgggccaatg aattagataa
     2221 gattgcagct agtgtagcga ttgttcatcg tagagatgct tttagagcgc atgagcatag
     2281 tgtggatata ctaaaggcat caggtgttag aattttaacc ccttatgtcc caattggttt
     2341 aaatggagat agtcagagag ttagcagctt ggtcgttcaa aaagttaaag gtgatgaagt
     2401 aattgaatta cctttagaca acttgattgt tagttttggc ttttctactt ctaataaaaa
     2461 tttacgttat tggaatttgg attataaacg gtctagtatc aatgtttctt cactttttga
     2521 gacgactcaa gaaggcgtgt atgctattgg agatgctgct aattatccag gaaaggtaga
     2581 attgattgca acaggatatg gggaagcacc agtcgctata aaccaagcta tcaattatat
     2641 ttatcctgat cgagataacc gtgtagtcca ttcaacatca ttgataaaat aactataaat
     2701 aaaactgata agtttataaa aaaggattat atatattgtt aaaaaaataa caaaacttcc
     2761 tatttatttt gtgatataat taactttgtc gttatgaatg atattaaata aatgaagcga
     2821 ggtattttat gacaaatacg gtaacaccaa aagagacggc tggttctttt atcaataaag
     2881 tgttaggtgg gacagcaact gcaatcgttg tagcactcat cccaaatgct atcctagcca
     2941 cttttttgaa accattttta tcttacggac ttgctgcaga gttcttacac atcgtgcagg
     3001 tattccaatt tttcacacca attatggcag gatttttgat tggtcaacag tttaaattca
     3061 ccccaatgca acagcttgct gttggaggtg ctgcatatat tggttctggt gcgtgggctt
     3121 acacagaagt tattcaaaaa ggagttgcta ctggtagttt ccaattgcgt gggattggtg
     3181 atctcatcaa tatgatgcta acagcagctt tagcagtttt agcagttaag tggtttggta
     3241 ataaatttgg ttccctaacc attatcttgt tacctattat cataggtaca ggcgtaggct
     3301 atttaggatg gaaattactc ccttacgttt catatgtgac aacacttatt ggtcaaggaa
     3361 ttaattcttt tactacttta caaccaattg cgatgtcaat tctaattgcg atggcttttt
     3421 caatgcttat tgtaagtcct atttcaacag tagcgattgg tctagcgatt ggtcttaatg
     3481 gtatgtcagc aagtgcagct tcaatgggag ttgcttcaac aacagcagtg ctagtttggg
     3541 caacgatgaa ggctaataag tctggagttc caattgctat tgctttaggt gcaatgaaga
     3601 tgatgatgcc aaatttccta aagcatccag taatggcgat tccaatgctt atgactgcta
     3661 cagtaagttc tctaacagtc ccattgttta aattggtggg gacaccagct tcgtctggtt
     3721 ttgggcttgt tggtgcagta ggtccgattg cttcatttga agcaggagca agtatgttga
     3781 ttgttatctt atcttggctc gttattcctt ttgcagtagg ttttgtatca cacaaaattt
     3841 gtaaagacat tctgaaactt tataaagacg atattttcgt ttttgaaggt caaaattaat
     3901 ttggaggttg tatgttagtt tatattgctg gttcaggcgc tatgggatgt cgttttggat
     3961 atcaaatttc taaaacaaat catgatgtta ttttacttga taattgggca gaccatatta
     4021 tggctattaa agaaaatggc ttgaaggtaa caggtgatac agaggacctt gttaaacttc
     4081 ctatcatgaa gccaactgat gcgacagaag aagcagattt aattattttg tttactaaag
     4141 caatgcagtt gccaaatatg cttcaagata ttaaaaaaat tattggtaag aaaacaaagg
     4201 tcttatgtct tttaaatgga ttaggacatg aagatgtcat tcgtcaatat atccctgaac
     4261 acaatatttt gatgggagta acagtttgga ctgcagggtt gaaaggacca gggcatgctc
     4321 atctggaagg agttggttct gtaaatcttc aaagtatcga tcctaacaat caagaagcag
     4381 gtcatcgcgt tactgaattg cttaatgaag caaaattaca ggctacttat gacgaaaatg
     4441 ttttgccaaa tatttggaga aaagcttgtg ttaacggaac gatgaattca acttgcgcct
     4501 tgctagattg tacaattggt caactctttg ctagtgaaga cggcgtaaat atggtacatg
     4561 aaattatcca tgaatttgtt actgttggaa aagcagaagg tgtagagctt gatgaagaag
     4621 aaataacaaa gtacgttatg gatacatctg ttaaagcagc ccatcattac ccttcaatgc
     4681 atcaagactt agttcaaaat cagcgcttaa ctgaaattga tttcttaaat ggcgcagtga
     4741 ataaaaaagg tgaaaaccta ggcattgata caccttactg tcgattaatc acacaattaa
     4801 ttcacactaa agaaaatgta cttagcataa aataataatc aaccgggaga atagtgcccg
     4861 gttttttgat atgcttaatg ataatgattt tgttatacta agaataataa gagttgggta
     4921 tttttttcga gaagtattta cagcataaaa tattacattt gtattacaaa tatttctaaa
     4981 atatgttgac atgttacaaa aaggatatta taatagtagt gtaaataaat aaggaagtag
     5041 gtgtttatga tgaaaaaagg acaagtaaat gatactaagc aatcttactc tctacgtaaa
     5101 tataaatttg gtttagcatc agtaatttta gggtcattca taatggtcac aagtcctgtt
     5161 tttgcggatc aaactacatc ggttcaagtt aataatcaga caggcactag tgtggatgct
     5221 aataattctt ccaatgagac aagtgcgtca agtgtgatta cttccaataa tgatagtgtt
     5281 caagcgtctg ataaagttgt aaatagtcaa aatacggcaa caaaggacat tactactcct
     5341 ttagtagaga caaagccaat ggtggaaaaa acattacctg aacaagggaa ttatgtttat
     5401 agcaaagaaa ccgaggtgaa aaatacacct tcaaaatcag ccccagtagc tttctatgca
     5461 aagaaaggtg ataaagtttt ctatgaccaa gtatttaata aagataatgt gaaatggatt
     5521 tcatataagt ctttttgtgg cgtacgtcga tacgcagcta ttgagtcact agatccatca
     5581 ggaggttcag agactaaagc acctactcct gtaacaaatt caggaagcaa taatcaagag
     5641 aaaatagcaa cgcaaggaaa ttatacattt tcacataaag tagaagtaaa aaatgaagct
     5701 aaggtagcga gtccaactca atttacattg gacaaaggag acagaatttt ttacgaccaa
     5761 atactaacta ttgaaggaaa tcagtggtta tcttataaat cattcaatgg tgttcgtcgt
     5821 tttgttttgc taggtaaagc atcttcagta gaaaaaactg aagataaaga aaaagtgtct
     5881 cctcaaccac aagcccgtat tactaaaact ggtagactga ctatttctaa cgaaacaact
     5941 acaggttttg atattttaat tacgaatatt aaagatgata acggtatcgc tgctgttaag
     6001 gtaccggttt ggactgaaca aggagggcaa gatgatatta aatggtatac agctgtaact
     6061 actggggatg gcaactacaa agtagctgta tcatttgctg accataagaa tgagaagggt
     6121 ctttataata ttcatttata ctaccaagaa gctagtggga cacttgtagg tgtaacagga
     6181 actaaagtga cagtagctgg aactaattct tctcaagaac ctattgaaaa tggtttagca
     6241 aagactggtg tttataatat tatcggaagt actgaagtaa aaaatgaagc taaaatatca
     6301 agtcagaccc aatttacttt agaaaaaggt gacaaaataa attatgatca agtattgaca
     6361 gcagatggtt accagtggat ttcttacaaa tcttatagtg gtgttcgtcg ctatattcct
     6421 gtgaaaaagc taactacaag tagtgaaaaa gcgaaagatg aggcgactaa accgactagt
     6481 tatcccaact tacctaaaac aggtacctat acatttacta aaactgtaga tgtgaagagt
     6541 caacctaaag tatcaagtcc agtggaattt aattttcaaa agggtgaaaa aatacattat
     6601 gatcaagtgt tagtagtaga tggtcatcag tggatttcat acaagagtta ttccggtatt
     6661 cgtcgctata ttgaaattta aagctaaatc tttttagatt tagctttact aatattatgt
     6721 gtgttggaaa aaacagtgga gatttacgtc aaaattatat tatttggata gtatcgaata
     6781 gatagaatgt ggagaaaagt aggaaatagt taagaaaggt atttatgacg cttcgagaat
     6841 taacaataga agaatttaaa gaacattcag gaaattatga ttcacaatca tttttacaaa
     6901 cacctgagat ggctaaactt ttagaaaaac gcggctatga tgttaggtat ttgggatatc
     6961 gagtagaaaa taaactagag ataatcagtt tatcttatat tatgccagtc actggtggtt
     7021 ttcaaatgaa aattgattca ggaccagttc attcaaattc taagtatcta aaacaatttt
     7081 ataaagcatt gcaaggctat gccaaatcca acggtgttct agaattaata gttgagcctt
     7141 atgatgatta ccaattattc actagttcgg gagttcctag taatcaggga aatgataatc
     7201 tgattgaaga ttttaccagt tcaggttatc accatgatgg tttaacaact ggttttactg
     7261 gtaaatattt atcttggcac tatgttaaaa atttagaagg tgtcacttct gaaacgttac
     7321 tatcttcatt ctctaagaca ggacgagctt tggttaagaa agcaatgtct tttggaatca
     7381 aggttcgcgt tcttaaacgt gatgagctac atttatttaa agagataaca acttctacgt
     7441 caaatagacg tgattatatg gataagtcct tagattatta tcaagatttt tacgatagct
     7501 ttgaaggcaa ggctgaattt gtgattgcca ctttaaattt tagagaatac gaccataact
     7561 tgcaaataaa agctgaagca ttggaaaata agcttaaatt gcttgatgaa cgttttaggg
     7621 aaaatgcaga ctctcctaag tatcataggc aacgctctga aataattaat caactggcta
     7681 gttttgaaac acgacgacaa gaagttcagt catttattca aaagtatgat aaccaagacg
     7741 ttgttttagc tggtagtcta tttgtttata gtcttaagga gacagtttat ttcttttcag
     7801 gatcctacac agaatttaat aaattttatg caccagcagt tcttcaggaa tatgttatgc
     7861 aagaggctct caaaagaggt tctactttct ataacctcct aggtattcag ggaacttttg
     7921 atggttcaga tagtattctt cgctttaaac agaattttaa tggttatatc attcgtaaaa
     7981 tgggaacttt taactattat ccaagtcctt ttaaatacaa aggaattcag cttcttaaaa
     8041 aggttttgaa gagaaattaa agtatttgta aaagctaatc tttagattag gcttttttag
     8101 tttattgata gtttttggaa gaaaatgctt gacattgaaa gaaaaagaga tataattaaa
     8161 ttgcaaacga tttcaattca aggagggact atgctaaagt caaagagaaa agaaattatc
     8221 ttatcacgac ttgagcaaaa taagagtgtt actttagatg aattaacgtc tatcttagag
     8281 acttctgaat ctacagttcg cagggattta gatgaacttg aatctgaagg ttttttaaaa
     8341 agggtgcatg gtggtgctga attaccatac tcacttggcc aggaattaag taatcaagaa
     8401 aaagctatca aaaacgttca aaaaaaattg gatattgcta gacaaactgc aaagctgata
     8461 gcaaagcaag atgttatatt tattgatgcc ggaactacga cagaattatt aattgatttt
     8521 ctccctcacg agcaattaac ggtggtgacg aattctattc atcatgctgc aaagttagtt
     8581 gatagaggga taaaaacaat cattatcgga ggagcggtta aacattcaac agatgctagt
     8641 attggccagg tagccattaa tcagattagg cagataacgg ttgacaaagc ttttctgggg
     8701 atgaatggta tagatgaagt ttatttaact acaccagatt tagaagaagc tgctattaaa
     8761 gaagctatta ttaataatag tcaacaaacc tttattctaa tggattcatc taaaattgga
     8821 caagttacat ttgctaaagt gaaagagata aatgacatta atttagtaac taataagact
     8881 gattcagaac ttatgacaat aataaaagag aaaatgaagg tgattcaagt atgatttata
     8941 cagtaacatt aaatccatcg attgatttta ttgttcgtct agatacacta ttgttgggta
     9001 gtgttaaccg tatgactagc gatgataaat atgtaggtgg taaaggaatt aatgttagtc
     9061 gcatattaaa acgcttaaaa attgacaata ctgctacagg ttttatcggt ggatttacag
     9121 gacattttgt tgaagatgga cttgtatcag aaggtatcaa aactgacttt gtttctgtta
     9181 atgaagatac acgtatcaac gttaaagtaa aagcaaaaat tgaaactgaa atcaatggcg
     9241 ggggtcctag gataacaaat gaacaacttc atcgtctaga gaaacttctt tcgagactaa
     9301 cacctgagga cacagtagtt ttcgcaggtt cagcaccagc tagccttgga aataaggtct
     9361 ataatacatt gattccaatt gctaaaaaga caggagcgga agttgtttgc gattttgaag
     9421 gtcagacact tttagacgct cttgcttatc agcctctttt agtaaaaccg aataaccatg
     9481 aattagcaga tatttttggc gtagagctag aaggtctgcc tgatatcgaa aaatatgcac
     9541 ataagattct agataaagga gctaagaatg ttatcgtttc tatggcaggg gatggtgcat
     9601 tgttagtaac tccagaagct tcatattttg caaaaccgat taagggtgaa gtgaaaaatt
     9661 ccgttggggc tggtgattca atggtagctg gctttacagg agaatttgtt aagtcaaaaa
     9721 atccagtaga agcactaaaa tggggcgtag cttgtggtac agcaaccact ttctcagatg
     9781 atttagcaac agctgagttt atccaagata tttataataa agttgaggta gaaaaattat
     9841 gaaaatccaa gacctactaa aaaaagaagt tatgattatg gatttaaaag caaccagtaa
     9901 agaggctgca attgatgaaa tgattacaaa attagttgac acaggtgttg tgacaaactt
     9961 tgctattttt aaagatggca tcatgaagcg tgaggcacaa acatcaactg gattaggtga
    10021 tggtatcgct atgccacata gtaaaaatgc tgccgttaaa gaagctactg tcctttttgc
    10081 aaaatctgca tcaggtgttg attacgaagc acttgatgga cagccaactg atttattctt
    10141 tatgattgct gcgccagatg gtgctaatga tacgcattta gctgccttgg cagaactatc
    10201 taaatatctt ttaaaagaag ggttcgctga tcaattacgt caagcaaaaa cgccagatga
    10261 cattattgct acttttgata gtaatagcat ttctcaagaa acagtagctc cacaaacagt
    10321 acaatcaaca agtaaaggtt cagattatat tgttgcagta acagcttgca ctacgggaat
    10381 tgcccacacg tatatggcag aagaagcttt aaagaaaaaa gcagctgaaa tgggtgtagg
    10441 tattaaagtt gaaacaaatg gcgcttctgg tgtaggaaat aagttgactt caagtgatat
    10501 tgcaagggct aagggtgtta ttattgcagc agataaggct gtagagatgg atcgttttga
    10561 tggcaaacct ttggtatcac gtccggtagc tgatggtatt aaaaaaagtg aagacttaat
    10621 taacattatt ctcgataata aggcacaaac ctatcatgca aaaaatcaaa atgataaaca
    10681 atcaggtgaa tctgatggaa aatcaggctt aggctctgct ttttataaac accttatggg
    10741 tggtgtatca caaatgttac cattcgttat tggtggtggt attatgattg cgattgcttt
    10801 cctttttgat aacatacttg gtgtaccaaa agaccaatta agtaatttag gttcatacca
    10861 tgaaattgca gccttattca aaaatatcgg tggcgcagca tttgcattta tgttaccagt
    10921 attggcaggt tatatcgctt attcaatagc tgaaaaacca ggactagtag ctggcttcgt
    10981 agctggttca attgcatcaa gtggactggc attcggtaaa gtaccttttg cagaaggtgg
    11041 gaaagcaacc ttagcattag caggtgtgcc atcaggtttc ttaggggctt tagtaggtgg
    11101 tttcttagct ggtggagtta tcctactact tagaaaatta ctttcaggtc tcccaaaatc
    11161 gcttgaaggt attaaatcaa ttcttctata cccattgtta ggtgttttga ttacaggttt
    11221 cttgatgttg ctcgtaaaca ttccaatggc agctatcaac acagctctta acactttctt
    11281 acaaggttta tctggtagtt cagcagtctt gatgggctta ctagttggtg gtatgatggc
    11341 tgtagatatg ggtgggcctg ttaataaagc agcttatgtt tttggtacag gtactcttgc
    11401 agcaactgtt gcaaatggcg gctctgttgt tatggcagct gtaatggctg gaggaatggt
    11461 tccaccgtta gcagtatttg tggcaacttt attatttaaa gataaattta ataatgaaga
    11521 acgtcaatct ggtctaacaa atatcgttat ggggttatct tttatcactg aaggtgctat
    11581 tccgtttggt gctgctgacc cagctcgtgc aatccctagc tttattgtag gttcagcctt
    11641 aactggagca cttgttggtt tagctggtat caagttgatg gctcctcatg gagggatttt
    11701 tgtcatcgca ttaacaagta atcctttact ttatattcta tttattctaa ttggagcggt
    11761 agtctcaggt gtactctttg gcttgttccg taaaacaaaa tagttaatct caacttatcc
    11821 aaatttacat tgaataaaag gtgaataaac tatcgttatt gggtctattc gcctttttat
    11881 gttgtaaaag aatggtagat taatcattaa taattgctaa tcttttttca ataggatatg
    11941 atttatttaa gcaaaaaaat ggataatatg attgaggaaa ggaggtaagc aatgattaaa
    12001 tggaatgaaa aagaaacaaa actagttgat acaagttttt caattatgtt gtatctaaaa
    12061 acgctatttg cagtcggaca agtagtagct ggcgttttat tatattattt taaacctaca
    12121 caaataaaac atctcctatt tcttttaact gaaggtgaag ttcacggtgg gattcatggt
    12181 tttatttctg ctcatttctt gaaaataagc agttatatta atgattcggc tagtcattta
    12241 gccgctatct atctattaat tcatggcggt atcaaattag tagcacttat tttattacat
    12301 aaagagaaat tatttgctta ccctttatca attttagttt tcgttgggtt tatcattttc
    12361 caactaaggg aatatttttc tagtcatagc attttaatga tttttctatg tgtatttgat
    12421 gttatcatga taattttaac atggttagaa tatcagaaaa tgaaattgaa tttttcaaaa
    12481 aggtgacttc ccccatgcct tagcgcttgt ttttataagc gcttttttgc tacaatatga
    12541 ctatgaaaac ta
//
LOCUS       DATRTN010000049        12466 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.049, whole genome shotgun sequence.
ACCESSION   DATRTN010000049 DATRTN010000000
VERSION     DATRTN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12466
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.049"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            152..691
                     /locus_tag="VBE22_001319"
     CDS             152..691
                     /locus_tag="VBE22_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO7202781.1"
                     /translation="MSITSVKKSKPFKLGVAGLLVGASLALPLSVSAASYTVKSGDTL
                     SAIAKNHKTTVQELVSLNSISNADVISIGDVLKLDNSTASQAEAKSQPTIENSMNSSS
                     NLSSSDSAAKEEIARRESNGSYTAQNGQYYGRYQLSQSYLNGDLSPENQEKVADNYVV
                     SRYGSWSAALSFWNSNGWY"
     gene            797..1501
                     /locus_tag="VBE22_001320"
     CDS             797..1501
                     /locus_tag="VBE22_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEO7202782.1"
                     /translation="MNKRRKLSKLNVKKHHLAYGAITLVALFSCILAVMVIFKSSQVT
                     TESLSKADKVRVAKKSKMTKATSKSKVEDVKQAPKPSQASNEAPKSSSQSTEANSQQQ
                     VTASEEAAVEQAVVTENTPATSQAQQAYAVTETTYRPAQHQTSGQVLSNGNTAGAIGS
                     AAAAQMAAATGVPQSTWEHIIARESNGNPNVANASGASGLFQTMPGWGSTATVQDQVN
                     SAIKAYRAQGLSAWGY"
     gene            complement(1636..2508)
                     /gene="sdaAA"
                     /locus_tag="VBE22_001321"
     CDS             complement(1636..2508)
                     /gene="sdaAA"
                     /locus_tag="VBE22_001321"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEO7202783.1"
                     /translation="MFYTIEELVEQANSQHKGNIAELMIQTEIEMTGRSREEIRYIMS
                     RNLEVMKASVIDGLTPSKSISGLTGGDAVKMDQYLQSGKTISDTTILAAVRNAMAVNE
                     LNAKMGLVCATPTAGSAGCLPAVISTAIEKLNLTEEEQLDFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSASAMAAAALVMAAGGTPFQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNALGSSFALVAADMALAGIESQIPVDEVIDAMYQVGSSLPTAFRETAEGGLAA
                     TPTGRRYSKEIFGE"
     gene            complement(2523..3191)
                     /gene="sdaAB"
                     /locus_tag="VBE22_001322"
     CDS             complement(2523..3191)
                     /gene="sdaAB"
                     /locus_tag="VBE22_001322"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEO7202784.1"
                     /translation="MKHLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGEPSE
                     VTFHLYNSFAKTYQGHGTDKALVAGILGMDTDNPDIKNSLEIAHQKGIKIYWDILKDS
                     NSPHPNTAKITVKNGDRSMSITGVSIGGGNIQVTELNGFSVSLTMNTPTLIIVHQDIP
                     GMIAKVTDILSDFNINIAQMNVTRESAGEKAIMIIEVDSRDCQQAVKKIEAIPHLHNV
                     NFFD"
     gene            3437..4558
                     /gene="mnmA"
                     /locus_tag="VBE22_001323"
     CDS             3437..4558
                     /gene="mnmA"
                     /locus_tag="VBE22_001323"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEO7202785.1"
                     /translation="MTDNSNIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVTRDENGIVHMLRGADNNKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKPEVRRIAEEAGLATAKKKDSTGICFIGEKNFKDFLGQYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNKPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLTASEIHFTRDMPNEFKLECTAKFRYRQPDSKVTVYVKGNQARVVFD
                     DLQRAITPGQAVVFYNEQECLGGGMIDQAYRDDKICQYI"
     gene            4590..5201
                     /locus_tag="VBE22_001324"
     CDS             4590..5201
                     /locus_tag="VBE22_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEO7202786.1"
                     /translation="MISKFILAFMAFFAIMNPISNLPAFMALVADDDQKISRRIAAKG
                     VLLAFVIIVIFVLSGHLLFNLFGITLAALKISGGILVGIIGYKMINGIHSPTNKNLEE
                     HKDDPMNVAVSPLAMPLLAGPGTIATAMGLSSGGLSGKLITILAFAILCVIMYVILIS
                     ANEITKFLGKNAMTIITKMMGLILMTIGIEMLITGIKIGFHLT"
     gene            5371..7272
                     /gene="mnmG"
                     /locus_tag="VBE22_001325"
     CDS             5371..7272
                     /gene="mnmG"
                     /locus_tag="VBE22_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEO7202787.1"
                     /translation="MTHNFAENYDIIVVGAGHAGVEASLAASRMGCKTLLATINLEML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KALYAQTMKQTVEKQENLTLRQAMIDEILVEDGKVVGVRTATNQKFSAKSVVITTGTA
                     LRGEIILGDLKYSSGPNNSLASVTLADNLRDLGLEIGRFKTGTPPRVKASSINYEKTE
                     IQPGDEQPNHFSFMSRDEDYITDQVPCWLTYTNTLSHDIINQNLHRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRYTEEVYVQGLSTSLPEDVQVDLLRSIKGL
                     ENAEMMRTGYAIEYDIVLPHQLRATLETKVIAGLFTAGQTNGTSGYEEAAGQGLVAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGYEIGLVDEERYAIFKKRQMQFENELERLDSIKLKPVSETNKRIQELGFKPLTD
                     ALTAKEFMRRPQITYAVATDFVGCADEPLDSKVIELLETEIKYEGYIKKALDQVAKMK
                     RMEEKRIPPHIDWDDIDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEG
                     RQKGRKNIN"
     gene            7339..9321
                     /locus_tag="VBE22_001326"
     CDS             7339..9321
                     /locus_tag="VBE22_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEO7202788.1"
                     /translation="MKRFRFATVHLVLIGLILFGLLAICVRLFQSYTALLLAIFVALS
                     FVVALLYYQKITYELSEVEQIELLNDQTEVSLKSLLEQMPVGVIQFDLETNDIEWFNP
                     YAELIFTGDNGHFQSATVKDIITSRRNGTAGQSFEYGDNKYSAYLDTETGVFYFFDNF
                     MGNRRNYDSSMLRPVIGIISIDNYDDIMDTMLEADMSKINAFVTSFISDFTQSKNIFY
                     RRVNMDRYYIFTDYSVLNTLIKDKFDILNEFRKRAQENHLSLTLSMGISYGDGNHNQI
                     GQIALENLNTALVRGGDQIVVRENDSSKKALYFGGGAVSTIKRSRTRTRAMMTAISDR
                     LKVVDSVFIVGHRKLDMDALGASVGMQFFASNIVNASYVVYDPNDMNSDIERAIDYLQ
                     EDGETRLVSVERAFELITQNSLLVMVDHSKTALTLSKEFFNKFADVIVVDHHRRDEDF
                     PKNAVLSFIESGASSASELVTELIQFQQAKDKLSRSQASILMAGIMLDTRNFASNVTS
                     RTFDVASYLRGLGSNSMAIQKISATDFDEYRLINELILKGERIYDNIIVATGEEHKVY
                     SHVIASKAADTMLTMAGIEATFVITKNSSNIGISARSRNNINVQRIMEKLGGGGHFSF
                     AACQIQDKSVKQVRRMLLEIIDEDLRENSTVENRRD"
     gene            9327..9779
                     /gene="rplI"
                     /locus_tag="VBE22_001327"
     CDS             9327..9779
                     /gene="rplI"
                     /locus_tag="VBE22_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEO7202789.1"
                     /translation="MKVIFLQDVKGKGKKGEVKEVPTGYAQNFLLKKNLAKEATTQAI
                     GELKGKQKSEEKAQAEILAQAKELKTQLESETTRVQFIEKVGPDGRTFGSITAKKIAE
                     ELQKQYGIKIDKRHIDLDHTIRAIGKVEVPVKLHKQVSSQIKLDIKEA"
     gene            9822..11177
                     /gene="dnaB"
                     /locus_tag="VBE22_001328"
     CDS             9822..11177
                     /gene="dnaB"
                     /locus_tag="VBE22_001328"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO7202790.1"
                     /translation="MVEVSELRVQPQDLLAEQAVLGSIFISPEKLIMVREFISPDDFY
                     KYSHKVIFRAMITLADRNDAIDAATVRNILDDQGDLQNIGGLGYIVELVNSVPTSANA
                     EFYAKIVSEKAMLRDIISKLTDTVNMAYEGNDSDEIIATAEKALVDINEHSNRSGFRK
                     ISDVLKVNYENLELRSQQTSDVTGLPTGFRDLDRITTGLHPDQLIILAARPAVGKTAF
                     VLNIAQNVGTKQNRPVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVT
                     IAQGALADAPIYIDDTPGIKITEIRARSRKLSQEVDDGLGLIVIDYLQLISGTRPENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYRREGEEAEEIVEDNTVEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFE
                     E"
     gene            11189..11476
                     /locus_tag="VBE22_001329"
     CDS             11189..11476
                     /locus_tag="VBE22_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEO7202791.1"
                     /translation="MSDAFADVAKMKKIKEDIKSHEGQMVELTLENGRKREKNKIGRL
                     IEVYPSLFIVEYKDTAAVPGAIDNTYVESYTYSDILTEKTLIRYFDDESAE"
     gene            11806..12417
                     /gene="rpsD"
                     /locus_tag="VBE22_001330"
     CDS             11806..12417
                     /gene="rpsD"
                     /locus_tag="VBE22_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEO7202792.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKAKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
BASE COUNT         4103 a         2014 c         2457 g         3892 t
ORIGIN      
        1 tacttaaaga aaactaaaaa ttgttttttg gaggcctttt ctttattaat ttacaattta
       61 tttgtaataa atttcatcta aggcaaaaca attagtaata tttacaaggt atgatagtac
      121 gcgtatcaat taatgaaaga gagcatatat tatgtcaata acctcggtta aaaaatcaaa
      181 accatttaaa ttaggagtgg caggtctttt agtgggtgct tcattagctt taccactttc
      241 agtaagcgca gcatcttata ccgtgaaatc aggtgatacc ttatcagcta ttgctaaaaa
      301 tcataaaact acggtacaag agttagtgtc tctcaatagt atcagtaacg ctgatgtcat
      361 cagtataggt gatgttttaa aattggataa ttctacagct agtcaagcag aagcaaaatc
      421 tcaaccaaca attgaaaatt caatgaattc ttcatcaaat ttgagttcaa gtgattcagc
      481 cgcaaaagaa gaaatagctc gtcgtgaatc aaatggtagt tatactgcac agaatggaca
      541 atattatgga agatatcaac tgtctcaatc ttacctaaat ggcgacttat ctcctgaaaa
      601 tcaagaaaaa gtagcggaca attatgtggt ttctcgttac ggatcttggt cggcagcgct
      661 atcattttgg aatagtaacg gctggtatta ataaaagttg ataaaacaaa gagatttact
      721 taaaattaaa tctctttgta atatttttaa catctattca attgcaaggt aacaaataaa
      781 gtataatata ggtaacatga ataaaagaag aaaattatca aaattgaatg taaaaaaaca
      841 tcatttagct tatggagcta tcactttagt agcccttttt tcatgtattt tggctgtaat
      901 ggtcatcttt aaaagttcac aagttactac tgaatctttg tcaaaagcag ataaagttcg
      961 cgtagccaaa aaatcaaaaa tgactaaggc gacatctaaa tcaaaagtag aagatgtaaa
     1021 acaggctcca aaaccttctc aggcatctaa tgaagcccca aaatcaagtt ctcaatctac
     1081 agaagctaat tctcagcaac aagttactgc gagtgaagag gcagctgtag aacaagcagt
     1141 tgtaacagaa aacacccctg ctaccagtca ggcacaacaa gcttatgctg ttactgagac
     1201 aacttataga cctgctcaac accagacgag tggccaagta ttgagtaatg gaaatactgc
     1261 aggggctatt ggctcagcag ctgcagcaca aatggctgct gcaacaggag tccctcagtc
     1321 tacttgggaa catattattg cccgtgaatc aaatggtaat cctaatgttg ctaatgcctc
     1381 aggagcttca ggacttttcc aaacgatgcc aggttggggt tcaacagcta cagttcagga
     1441 tcaagttaat tcagctatta aagcttatcg tgctcaaggt ttatcagctt ggggttacta
     1501 gtgaatagct ataaattaac tttacactgt tgtcaacaat aattacagat tgctaaaaca
     1561 aataaataaa aacgctacag taaagctgta gcgtttttat ttattttcag ggaaagagta
     1621 gaaaattgat aaactttatt ccccaaaaat ttctttacta taacgtcttc ctgtcggcgt
     1681 ggcagcaagt cctccttctg cagtctcacg aaaagcagtc ggtaaacttg atccaacttg
     1741 atacattgca tcaataactt catctactgg aatttgcgat tcaataccag ccaaggccat
     1801 atcagcagca acaagtgcaa aacttgatcc aagagcattc cgcttcacac aagggacttc
     1861 aactaaacct gcaacagggt cacagataag tccaagcata tttttaataa caaatgctat
     1921 agcttggcta gcttggaaag gagtacctcc agcagccata actaaagcag ccgcagccat
     1981 agcactagct gacccaactt cagcttggca acctccttct gcacctgaga tagaggcatt
     2041 attaccaatg acgagaccaa atgcgccggc tgtaaataga aaatcaagtt gctcttcttc
     2101 tgttaaatta agcttttcaa tggctgtaga aatcacagct ggtaaacatc ctgcactacc
     2161 tgcagttggt gttgcacaga ccagtcccat cttagcattt aactcattaa cagccatagc
     2221 attcctaacg gcagctagga ttgtggtatc tgaaatagtt tttcctgatt gtaaatattg
     2281 atccatcttg acagcatcac cgcctgttaa accactgatt gatttactag gggttaatcc
     2341 atcaataaca gaagctttca tgacttcaag atttcgggac ataatataac gaatttcttc
     2401 acgacttcta ccagtcattt caatttccgt ttggatcatg agctctgcta tgttaccctt
     2461 atgttggcta ttagcttgct ctaccagctc ttcaattgta taaaacatag ttaccctttc
     2521 tcttaatcaa agaagttgac attgtgtaaa tgaggaatag cttctatctt tttgacagct
     2581 tgttgacaat ctcgtgaatc tacttctata atcatgatag ctttttctcc tgcactttcg
     2641 cgtgttacat tcatttgagc aatattaatg ttaaagtcag ataaaatatc agttacttta
     2701 gcaatcatgc caggtatatc ttgatgaacg ataattaaag taggagtatt cattgttaat
     2761 gaaactgaga atccattcaa ctcagtaact tggatgtttc cgccaccaat tgacactcct
     2821 gtaatactca tgctcctatc cccattttta acagttattt tcgcagtatt tggatgtggt
     2881 gaattactat cttttaggat atcccagtaa atcttaattc ctttttgatg agcaatttca
     2941 agagagtttt taatatctgg attatctgta tccattccta gaatccctgc aaccaatgct
     3001 ttatcagtac cgtgtccttg gtaagtttta gcaaaagaat tgtataaatg aaaggttact
     3061 tcactaggtt caccaaaaat agaatggaca actttaccaa tgcggacagc tcctgcagta
     3121 tgactacttg atggtccaat cataacagga ccaataatgt cgaagaccga ttgaaatttt
     3181 aaatgtttca tagtttttcc tcgtgatttt tgtacattct cattataaca gattatttct
     3241 aaaaaatatc ataaaaagat aaaaaaactg caatgtaata aatatttatc acaacaaaaa
     3301 catacaactt gacaacaaat agtgctataa tatataagca cttttatagt gtttcttagt
     3361 caatatgatt gatctaagaa caacttggcc cttcaagcct aagttaaaaa taaaactttt
     3421 tgatgggaga attcttatga ctgataattc aaacattcgt gtcgttgtag gcatgagtgg
     3481 aggtgttgat tcatcagtaa ctgccctgct tttaaaagag caaggttatg atgttattgg
     3541 tgtttttatg aaaaattggg atgacacaga tgaatttggt gtttgtacgg ctactgaaga
     3601 ttataaagat gttgctgctg ttgctgatca gatcggcata ccatactact ctgtcaactt
     3661 tgaaaaagag tattgggaca gggttttcga atatttttta gcagaatatc gagcaggtcg
     3721 tacgccaaat ccagatgtta tgtgtaataa ggaaattaaa tttaaagcat ttctagatta
     3781 tgcgatgaca ttaggtgctg actatgtagc aacaggtcat tatgcacaag taacacgtga
     3841 tgagaacgga atagttcata tgcttagagg agcagataat aataaggatc aaacttattt
     3901 tctaagtcaa ttatctcagg agcaattgca aaaaacgtta tttccacttg gtcatttaca
     3961 aaaaccagaa gttcgccgta tagctgaaga agcaggactt gcgaccgcta agaaaaaaga
     4021 ctctactggt atttgtttta ttggagaaaa aaatttcaag gatttcctgg gtcaatattt
     4081 accggctcaa ccaggtcgta tgatgactgt tgatggtcgt gacatgggtg aacatgctgg
     4141 tttaatgtat tatactattg gtcaaagagg gggccttggt attggtgggc aacatggtgg
     4201 agataataaa ccttggtttg tagttggaaa ggatttaagt aaaaacattc tttatgtagg
     4261 tcaaggtttc tatcatgact cattgatgtc aactagcttg acagcttctg aaatccactt
     4321 tacacgtgat atgcctaatg aatttaagct agaatgtaca gctaagttca gatatcgtca
     4381 acctgacagt aaagttacag tttacgtaaa aggtaatcaa gcaagggttg tgtttgatga
     4441 tttacaacga gcaatcacac caggtcaagc tgttgttttt tataatgaac aagaatgttt
     4501 aggcggtggg atgattgatc aggcttatcg tgatgataaa atctgtcaat acatttaatt
     4561 gacacttaat tattaagtga ggtaatcgta tgatttcaaa atttatttta gctttcatgg
     4621 cttttttcgc cattatgaat cccatttcta atttgccagc ttttatggct ttagtagctg
     4681 atgatgatca gaagatttca cgtcggattg cagcaaaagg agtattactt gcttttgtca
     4741 ttattgtaat ctttgtatta agtggccact tactttttaa cttatttggg attaccctag
     4801 ccgccctaaa aatatctggt ggaatattgg taggcattat aggttataaa atgattaatg
     4861 gtatacattc accaaccaat aaaaacctag aggagcataa ggatgatcca atgaatgtgg
     4921 cagtttcacc attagcaatg ccccttttgg caggtccggg tactattgca actgctatgg
     4981 ggttatcaag tggtggttta tctggcaaac tcattactat acttgctttt gccatattat
     5041 gtgtgataat gtatgttatt ttaataagtg caaacgaaat tactaaattt cttggaaaaa
     5101 atgcaatgac tataatcacc aaaatgatgg gacttatttt gatgactatc gggatagaga
     5161 tgctaataac aggcataaaa attggttttc atttaacata gcctatcccc tctggttgac
     5221 ttttaaaaga taaatgctaa aataaattta acaataacta tgatggtcaa agctcatgga
     5281 atttgtaagt ctttttgacg tacaattttc gagagttttg gctatttttg tgttttaatg
     5341 aattagactt tgaaattgga aaggaataaa atgacacata attttgcaga aaactatgat
     5401 attatcgttg ttggagcggg tcatgcaggt gtggaagcta gtctagctgc tagtcgtatg
     5461 ggatgtaaga cacttctagc cactatcaac ttagaaatgt tagcatttat gccttgtaat
     5521 ccttctatag gtggctctgc taaaggtatc gttgtcagag agattgacgc tttaggtggt
     5581 gaaatgggta aaaacattga taaaacctat atacaaatga aaatgcttaa tacaggtaag
     5641 gggcctgctg tccgtgcctt aagagcacaa gctgataaag ccctctatgc acaaacaatg
     5701 aagcaaactg tagaaaagca agaaaatctt accttaagac aagctatgat tgacgaaata
     5761 cttgttgaag atggaaaggt tgtgggggtt cgtacagcaa ctaatcagaa attttcagca
     5821 aaatctgttg ttataacaac aggtactgct cttcgaggag agattatatt aggtgatctt
     5881 aagtactcct cgggccctaa taatagttta gcttctgtga cattggcaga taatttaaga
     5941 gacctaggat tagaaattgg tcgtttcaaa actggaacac ctcctcgtgt aaaagcctcc
     6001 tctattaatt atgaaaagac agagatacaa cctggggatg agcaaccaaa tcatttttca
     6061 tttatgtcta gggatgaaga ttatattact gaccaggtac cgtgctggtt gacctatacg
     6121 aatactttga gtcatgacat catcaatcaa aatctacacc gtgctcctat gttttctgga
     6181 atcgttaagg gagttggtcc tagatattgt ccgtcaattg aagataaaat tgtgcgtttt
     6241 gctgataaag agcgccacca actcttctta gaaccagaag gtagatacac tgaagaagtt
     6301 tatgttcaag ggctatcaac gagtcttcct gaggatgttc aagttgattt attacgttct
     6361 ataaaagggc ttgaaaatgc tgaaatgatg cgtacaggct atgctattga gtatgatatt
     6421 gtattgcctc atcaattaag agcaacactc gaaacaaaag taattgctgg tttgtttact
     6481 gcaggacaga ctaatggtac ttcaggttat gaagaagctg ctggtcaagg gttggttgct
     6541 gggatcaacg ctgctcttaa agttcaaggg aagccagaac ttattttaaa acgaagtgat
     6601 gcttacattg gtgtcatgat tgatgattta gttaccaaag ggactctaga accatatcgt
     6661 ctgttgacaa gtcgcgcaga atatcgtctg attcttcgtc atgataatgc agatatgcgt
     6721 ttaacagaaa tcggttatga gattggccta gtagatgaag agcgttatgc gatatttaaa
     6781 aagcgtcaaa tgcaatttga aaatgaatta gaacgtttag actctataaa gttaaagcca
     6841 gtttctgaga caaataaacg gattcaagaa ttgggcttta aacctttgac agatgcttta
     6901 actgccaaag aatttatgcg ccgcccacaa atcacatatg cagtagcaac ggattttgtt
     6961 ggttgtgctg atgagccatt agacagtaag gttattgaac ttttggaaac tgaaattaaa
     7021 tacgaaggct atattaaaaa ggctttagat caagttgcaa aaatgaaacg aatggaagaa
     7081 aaacgtattc cccctcatat tgactgggac gacattgatt ctatcgcaac agaagctaga
     7141 caaaaattca aaaaaattaa cccagaaacg ctaggtcagg ctagtcgtat ttcaggagtt
     7201 aatccagccg atattagtat tttaatggtt taccttgaag gaagacaaaa aggtcgtaaa
     7261 aatatcaatt gataatagtg ttcgcccctg tttatcaggg gcgttttgtg ttataattag
     7321 gtcttaagag gttatttaat gaaaagattt cgttttgcaa ccgtccattt agttttaata
     7381 ggcctgattt tatttggact attagctatt tgtgttaggt tatttcaatc ctatacggct
     7441 ctattattgg ctatttttgt tgctttatct tttgttgttg ccttattata ttaccaaaag
     7501 ataacttatg aactatctga agttgaacaa attgaattac ttaatgatca aacagaagtt
     7561 tctcttaaga gtcttcttga acaaatgcca gttggtgtta tacaatttga tcttgaaaca
     7621 aatgatattg agtggtttaa cccttatgca gaattgattt ttactgggga taatggtcat
     7681 tttcagtctg ctactgttaa agatatcatt actagtagac gtaatggaac ggctgggcaa
     7741 tcatttgaat acggtgataa caaatattca gcttatttgg atacagagac gggtgttttc
     7801 tatttcttcg ataatttcat ggggaataga agaaactatg atagttcaat gctacgtcca
     7861 gttattggta ttatttccat tgataactac gatgatatta tggatactat gttagaggct
     7921 gatatgtcaa agattaacgc cttcgtgact agttttattt ctgattttac acagtctaaa
     7981 aatatttttt atcgtcgtgt caatatggat cgatactata tttttaccga ctatagcgtt
     8041 ttaaatacat tgataaaaga taaatttgat attttaaatg agtttcgaaa acgcgctcag
     8101 gaaaaccatt tatcattaac attaagtatg ggaatttctt atggtgatgg taaccataat
     8161 caaattgggc aaattgcttt agaaaatctt aatacagcac tcgttcgcgg aggagatcaa
     8221 atagtagtac gtgaaaatga tagtagtaaa aaagcacttt actttggtgg tggtgctgtt
     8281 tcaactatta aacgttcacg aacacgtacc agagctatga tgactgctat ttctgaccgt
     8341 ttgaaagtag ttgatagtgt ctttattgtg ggacacagga aattggacat ggatgcacta
     8401 ggtgcatctg tagggatgca attttttgct agtaatattg ttaatgccag ttatgtagtt
     8461 tatgacccaa atgacatgaa ttctgatatt gaaagagcaa ttgattactt gcaagaagac
     8521 ggcgaaacac gtctcgtaag tgttgagaga gcttttgaat taattactca aaattcttta
     8581 ttagtcatgg tagaccattc taaaacagct ttaaccttat ctaaggaatt ctttaacaag
     8641 tttgcagatg ttattgtggt agatcatcat agaagagatg aagattttcc aaaaaatgct
     8701 gttctttcat ttatagaaag tggcgcaagt agtgccagcg aattagttac agaattgatt
     8761 caatttcaac aagctaaaga taaattaagc cgcagtcaag caagtatttt aatggctggt
     8821 atcatgcttg atacaagaaa ttttgcatct aatgtaacga gtagaacttt tgatgtggca
     8881 agctatttac gcggtttagg tagtaatagt atggctattc aaaaaatatc agctacagat
     8941 tttgatgaat atcgtttaat taatgaattg attttaaaag gtgagcgtat ttatgataac
     9001 atcattgttg ctaccggaga agaacataaa gtttactcgc atgttatagc tagtaaagca
     9061 gctgacacca tgttgacaat ggcaggtatt gaggctactt ttgttattac taaaaacagt
     9121 tcaaatattg gtatttcagc gcgcagtaga aataatatca atgtccagcg tattatggaa
     9181 aaacttggtg gtggcggaca ttttagtttt gctgcttgtc aaatacaaga caagtctgta
     9241 aagcaagtta gacgtatgtt actggaaata attgacgaag atttaagaga aaatagtaca
     9301 gtcgaaaata gaagggatta attgagatga aagttatttt tttacaagat gttaagggga
     9361 aaggaaaaaa aggtgaggtt aaggaagtac ctactggtta tgctcaaaac ttcttattaa
     9421 aaaagaatct agctaaagaa gcaacgacac aagctatcgg tgaattaaaa ggaaaacaaa
     9481 agtccgaaga aaaagcacaa gcagaaattt tagcacaggc aaaggagctg aaaacacaat
     9541 tagaatccga aactacccga gtgcaattta ttgaaaaggt aggtccagat ggtcgtacgt
     9601 ttggttctat cacagccaaa aagattgctg aagagttaca aaaacaatac ggcattaaaa
     9661 ttgacaaacg tcatattgat ttagatcata ctattagagc gattggtaag gttgaagtac
     9721 cagtaaaatt acataaacag gtttcttcac agattaagct tgatatcaaa gaagcgtaaa
     9781 ctgagagatg ataaaggttg acaagtcagg aggaatgaag tttggtagaa gtatctgagc
     9841 taagagtaca acctcaagac ctgttggcag aacaggccgt tctaggatct atctttatct
     9901 caccagagaa actgattatg gtgagagaat ttataagtcc agatgatttt tataaatatt
     9961 cacacaaagt tatcttcaga gcgatgatta ccttagctga tagaaatgat gcaattgatg
    10021 ctgctactgt tagaaatatt ttagatgacc aaggtgatct tcaaaacatt ggtgggttgg
    10081 gctatattgt tgaattagtt aatagtgttc caacaagtgc taatgcggag ttttatgcca
    10141 aaatagtttc tgagaaagcc atgttgcgag atatcatttc taagttgaca gatactgtca
    10201 acatggccta tgaaggaaat gattctgatg aaattattgc caccgctgag aaggctttag
    10261 ttgatattaa cgaacacagt aatcgtagcg ggtttagaaa gatatctgat gttttaaaag
    10321 ttaattacga aaacttagaa ttacgttcac agcaaacctc agatgtcaca ggtttaccaa
    10381 ctggatttag agatttagac aggattacta caggtttaca tccagatcaa ttaatcatct
    10441 tagcggcccg tcctgcagta ggtaaaacag cctttgtttt aaatattgct caaaatgttg
    10501 gaactaaaca aaatagacct gtagctattt tttcacttga aatgggggct gagagtttag
    10561 ttgaccgtat gttggcagca gagggaatgg ttgattctca tagtttacga actggtcagt
    10621 tgacggatca agattggaat aatgtaacga ttgctcaagg agctttggcg gatgcgccaa
    10681 tttatattga tgatacgccc gggattaaaa ttacagaaat tagagcacgc tctaggaagt
    10741 tatctcaaga agttgatgat ggtttaggtt taatcgtcat tgactacctc caattaattt
    10801 caggaactag accagaaaat cgtcagcaag aagtttctga aatttcaagg cagttgaaga
    10861 ttttagctaa agaattaaag gttcctgtta tcgcattaag ccagctctct cgtggggttg
    10921 agcaaaggca ggataaacga ccggtcttat ctgatattcg tgaatctgga tcgattgaac
    10981 aagatgctga cattgttgct ttcttatatc gagatgatta ttatcgtaga gagggcgaag
    11041 aagcagagga aattgtagaa gataatactg ttgaggttat tttagaaaag aaccgtgctg
    11101 gtgcgcgtgg gacagtaaaa ttaatgtttc aaaaggaata taataaattt tctagtatag
    11161 ctcaatttga agaataggag atgttaatat gagtgatgca tttgcagatg ttgcaaaaat
    11221 gaaaaaaata aaagaagata tcaagtcaca tgagggacag atggtcgaac ttactctcga
    11281 aaatggtcgt aagagggaaa aaaataaaat tgggcgccta attgaagttt acccatcttt
    11341 gtttatcgta gagtataagg atactgcagc tgtaccagga gcaattgata atacttatgt
    11401 agagtcttac acttattcag atattttaac tgagaaaact ttaattcgtt attttgatga
    11461 cgagtcagca gaatagtatg aaaacctcga ctgaggtttt ttgtttctat aaaaaaattt
    11521 actaattctc tattggtggt tagaattaag aatctttctt attataattg gaaatactta
    11581 ttagatgtct atttgtattt aaggtttaag agacataaaa acttgattca atattatttt
    11641 catgatataa tatagcttgt gtgaaataac agcagaaaaa tatgaagctc gtcaacaggt
    11701 ggttaactaa tctagtaccc tttgctgttt aggcgaaaat cccctgcacg aatcaggatt
    11761 ttttaaatcg ttatttcctc aattatttta gaggaggaca tttagatgtc acgttatact
    11821 ggtccatcat ggaaacaatc acgtcgcctt ggtttatcac ttacaggcac aggtaaagaa
    11881 ttggcacgtc gtaactacgt acctggtcaa cacggtccta ataaccgtag caagctttca
    11941 gaatatggct tgcagttagc tgagaaacaa aaacttcgtt tttcatacgg acttggtgaa
    12001 aaacaattcc gtaacttgtt cgtacaagct actaaagcaa aagaaggaac tcttggtttc
    12061 aactttatgg ttcttttaga gcgtcgtctt gataacgttg tttatcgttt aggacttgca
    12121 acaactcgtc gccaagctcg tcaatttgtt aaccatggtc atatccttgt tgatggtaaa
    12181 cgtgttgata tcccatcata ccgcgttact ccaggacaag ttatttcagt tcgtgaaaaa
    12241 tcaatgaaag tacctgcaat ccttgaagct gttgaagcta ctttaggacg tccagctttc
    12301 gtatcatttg atgctgaaaa acttgaaggt tcattgactc gccttccaga acgcgatgaa
    12361 attaacccag aaatcaatga agcacttgtc gttgaattct acaacaaaat gctttaatta
    12421 ttacattaca tactttaaaa gcctatttta taggcttttt ttgcta
//
LOCUS       DATRTN010000050        12461 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.050, whole genome shotgun sequence.
ACCESSION   DATRTN010000050 DATRTN010000000
VERSION     DATRTN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12461
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.050"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            126..479
                     /gene="yaaA"
                     /locus_tag="VBE22_001331"
     CDS             126..479
                     /gene="yaaA"
                     /locus_tag="VBE22_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEO7202793.1"
                     /translation="MDYKLFDEYITLQSLLKEIGIIQSGGAIKKFLADNRVLFNGDLE
                     NRRGKKLRLGDIITIPDQNIEIIIRKPSDQEIEERNIEIAEKQRVSAIVKEMNKNTNK
                     GKSKTSKKPVRFPGT"
     gene            482..1591
                     /gene="recF"
                     /locus_tag="VBE22_001332"
     CDS             482..1591
                     /gene="recF"
                     /locus_tag="VBE22_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEO7202794.1"
                     /translation="MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIY
                     FLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGHLNLDIQLSEKGRITKVNHLKQA
                     KLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKPTYLAELSNYNHVLKQR
                     NTYLKTTNNVDKTFLTVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHL
                     SIHYKSSIEFTDKSSIREHFLNQLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFA
                     SQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQLKLLEGIKENVQTF
                     ITTTSLEHLSALPDQLKIFNVSDGTISINEKKATD"
     gene            complement(1603..2469)
                     /locus_tag="VBE22_001333"
     CDS             complement(1603..2469)
                     /locus_tag="VBE22_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEO7202795.1"
                     /translation="MEGLLIALIPMFAWGSIGFVSNKIGGRPNQQTFGMTLGALLFAI
                     IVWLFKQPEMTASLWIFGILGGILWSVGQNGQFQAMKYMGVSVANPLSSGAQLVGGSL
                     VGALVFHEWTKPIQFILGLTALTLLVIGFYFSSKRDVSEQALATHQEFSKGFATIAYS
                     TVGYISYAVLFNNIMKFDAMAVILPMAVGMCLGAICFMKFRVNFEAVVVKNMITGLMW
                     GVGNVFMLLAAAKAGLAIAFSFSQLGVIISIIGGILFLGETKTKKEQKWVVMGILCFV
                     MGAILLGIVKSY"
     gene            complement(2525..3187)
                     /locus_tag="VBE22_001334"
     CDS             complement(2525..3187)
                     /locus_tag="VBE22_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7202796.1"
                     /translation="MKNYGKILKELREDKGISLSSLAKSAQLSKSTLSRFENGETQIG
                     IDKFIKALQTLEVGVTINEVSILDSKVKAGTSNTDLEQLTLLESYRDNEDIMRIFSFQ
                     KQQSCDRIESNVLKILAKLFISNLGLNMRLPQDEINLVVTYLNGVTQYNDFYFKVICY
                     FQDILPEDVILNKISNMTKEQLPYSKSLVNLLIKQVIIALEKDSVDKAIVFADLANKV
                     SQ"
     gene            complement(3262..4743)
                     /gene="guaB"
                     /locus_tag="VBE22_001335"
     CDS             complement(3262..4743)
                     /gene="guaB"
                     /locus_tag="VBE22_001335"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEO7202797.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNEVDMKTKLADNLTL
                     NIPIITAAMDTVTDSKMAIAIARAGGLGIIHKNMSIVDQAEEVRKVKRSENGVIIDPF
                     FLTPDNTVSEAEELMQNYRISGVPIVETLENRKLVGIITNRDMRFISDYKQLISEHMT
                     SQNLVTAPIGTDLETAERILHEHRIEKLPLVDDEGRLSGLITIKDIEKVIEFPKAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQITAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIVFQMLGGIRSGMGY
                     VGAANIKELHDNAQFVEMSGAGLKESHPHDVQITNEAPNYSVH"
     gene            complement(4900..5385)
                     /locus_tag="VBE22_001336"
     CDS             complement(4900..5385)
                     /locus_tag="VBE22_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO7202798.1"
                     /translation="MNKKETRHQLIRSLVSETKVRTQHELRELLEKNGVSVTQATLSR
                     DMKELNLIKVNESSDNATETYYEIHSISQKRWEERLRFYMEDALVMLFPVQNQIVLKT
                     LPGLAQSFGSILDSILLPEILATVCGDDTCLIICNNSEEAQKCFEKLSQYTPPFFFSK
                     K"
     gene            complement(5395..6075)
                     /locus_tag="VBE22_001337"
     CDS             complement(5395..6075)
                     /locus_tag="VBE22_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO7202799.1"
                     /translation="MITKEQYFYFRQLDDFKHFTIEQFDHIVSHIKHRTALKNHTLFF
                     EGDYREKLFLIQSGHVKIEQSDASGSFIYTDYVRQGTVFPYGGLFLDDDYHFSAVAIT
                     DIEYFSLPMALYEEYSLQNINQMKHLCRKYSKLLRVHEIRLRNMVTSSASMRVIQSLA
                     TLLLQVPTERGHLPFPITTIEIANMSGTTRETVSHVLKELRQKDIVEMKGKKLLYNNK
                     NYFKKFIE"
     gene            6283..6987
                     /locus_tag="VBE22_001338"
     CDS             6283..6987
                     /locus_tag="VBE22_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEO7202800.1"
                     /translation="MTKFIVDSSYWNLFPTSKIGVILIKDYHMDRNLETELKQLLSDS
                     HSLAKKYLQEKEFSQNRVIQTYRKAYQTFKTKKGARSSIEALLKRVNSGNEITSINPL
                     VDIYNAASLRFGLPIGAEDSDTFRGDLKLTITNGGDEFYLIGEDFNRPTLSGELAYVD
                     DVGAVCRCFNWRDGKRTMITDNTQNAFLVIELIDNGREIIFKEALDFIATNTNRFLKA
                     KTQTIILDKEHSEITL"
     gene            7254..8486
                     /gene="arcA"
                     /locus_tag="VBE22_001339"
     CDS             7254..8486
                     /gene="arcA"
                     /locus_tag="VBE22_001339"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEO7202801.1"
                     /translation="MTQTHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRNEGVEVLYLENLAAESLTNQEIREQFIDEYIGEANVRGRA
                     TKKAIRELLLNIKDNKELIEKTMAGIQKSELPEIPSSEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGNGVSLNHMFSETRNRETLYGKYIFTHHPEYGGKVPMVYEREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVYSVTYENQDLHIEEEKGDLADLLAKNLGVEKVELI
                     RCGGDNLVAAGREQWNDGSNTLTIAPGVVIVYNRNTITNAILESKGLKLIKINGSELV
                     RGRGGPRCMSMPFEREDL"
     gene            8492..8923
                     /locus_tag="VBE22_001340"
     CDS             8492..8923
                     /locus_tag="VBE22_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7202802.1"
                     /translation="MKLLVNHLPYQRAASLYIRFSVFVIERNIKMEEEFDDNDEQDTI
                     YAVLYDGKQPVSTGRFLPETQTEARLTRIATLKGYRGNGYGTKIIIALENYAKENGYH
                     YLTIHAELTAKDFYQTLGYQATGNIYMEDGEACQTLEKYLI"
     gene            8939..9952
                     /gene="argF"
                     /locus_tag="VBE22_001341"
     CDS             8939..9952
                     /gene="argF"
                     /locus_tag="VBE22_001341"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO7202803.1"
                     /translation="MTQVFQGRSFLAEKDFSREEFEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTIKENFGKLEGITLVY
                     CGDGRNNVANSLLVAGTLMGVNVHIFSPKELFPAEEIVKLAEEYAKESGAHVLVTDNV
                     DEAVKGADVFYTDVWVSMGEEDKFKERVELLQPYQVNMELIKKANNDNLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            10015..11442
                     /gene="arcD"
                     /locus_tag="VBE22_001342"
     CDS             10015..11442
                     /gene="arcD"
                     /locus_tag="VBE22_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEO7202804.1"
                     /translation="MEKEKKLGLLPLTMLVIGSLIGGGIFDLMQNMSSRAGLVPMLIA
                     WVITAIGMGTFVLSFQNLSEKRPDLTAGIFSYAKEGFGNFMGFNSAWGYWLSAWLGNV
                     AYAALLFSSLGYFFKFFGNGNNIISIIGASIVIWVVHFLILRGVNTAAFINTIVTFAK
                     LVPVIIFLISALLAFKFNIFSLDIWGNGLHQSIFNQVNSTMKTAVWVFIGIEGAVVFS
                     GRAKKHSDIGKASILALFTMISLYVLISVLSLGIMSRPELANLKTPAMAYVLEKAVGH
                     WGAILVNLGVIISVFGAILAWTLFAAELPYQAAKEGAFPKFFAKENKNKAPINSLLVT
                     NLCVQAFLITFLFTQSAYRFGFALASSAILIPYAFTALYQLQFTLREDKSTPGHQKNL
                     IIGILATIYAVYLIYAGGFDYLLLTMIAYTLGMILYIKMRKDDKLPIFVGYEKISAIV
                     ILALCLLCIIEIMTGQIDIAAILAG"
     gene            11463..12419
                     /gene="arcC"
                     /locus_tag="VBE22_001343"
     CDS             11463..12419
                     /gene="arcC"
                     /locus_tag="VBE22_001343"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO7202805.1"
                     /translation="MVKEHQKIVVALGGNAILSTDASAKAQQEALINTSKSLVKLIKE
                     GHDVIVTHGNGPQVGNLLLQQAASDSEKNPAMPLDTCVAMTEGSIGFWLQNALNNELQ
                     EQGIDKEVATVVTQVIVDEKDQAFTNPTKPIGPFLSEEDAKKQAQETGSKFKEDAGRG
                     WRKVVPSPKPVGIKEASVIRRLVDSGVVVISAGGGGVPVIEDANTKALKGVEAVIDKD
                     FASQTLSELVDADLFIVLTGVDNVFVNFNKPNQEKLEEVTVSQMKQYITENQFAPGSM
                     LPKVEAAIAFVENKPESRAIITSLENIDNVLAQNAGTQIVAN"
BASE COUNT         4178 a         2258 c         2093 g         3932 t
ORIGIN      
        1 ttgacaaagt cgtcaaccaa tatttactcc cattttttat accttgtctc catatcataa
       61 gttcaactca aaaaataatt ttatgctata atgatgtgat atctaaaatt ggagattaaa
      121 aagtcatgga ttataaatta tttgatgaat atattacttt acaatcactt ctaaaagaga
      181 ttggtatcat tcaaagtggc ggagccatta aaaaattttt agcggataat cgagtattgt
      241 ttaacggtga ccttgaaaat aggcgtggaa agaaactccg cctgggggat attattacta
      301 ttcctgatca aaatatcgag atcattataa ggaagccatc agatcaagaa attgaagaac
      361 gtaatataga aatagctgaa aaacagcgtg tttcagcaat cgttaaagaa atgaataaaa
      421 acaccaacaa aggtaaatca aaaactagca aaaaaccagt tcgctttcca gggacataaa
      481 aatgtggata aaaaatattt ctttaaaaca ttatcgtaat tatgaagaag cacaagtaga
      541 tttttcaccc aacctcaata ttttcattgg ccgcaatgct caaggaaaga caaatttttt
      601 agaagcaatc tacttcttgg ctttgactcg aagtcaccgt acccgttctg ataaagaact
      661 tgtacatttt aaacaccatg atgtacaaat tacaggtgag gtaatccgca aaagtggcca
      721 tcttaactta gatattcaat tatctgaaaa aggaagaatc actaaagtta accatcttaa
      781 acaggcaaaa ttatctgatt atattggggc tatgactgtc gtactttttg cccctgaaga
      841 tcttcaatta gttaaaggtg cacctagtct aagacgaaaa tttttagata tagatattgg
      901 acaaattaag ccaacgtact tagctgaact ttcaaattac aatcatgtat taaaacaaag
      961 aaacacttat ctaaaaacta ctaataacgt cgacaaaact tttttaacag tccttgatga
     1021 acaattagct gattatggaa gtcgtgtcat tgaacaccgt tttgatttta tccaagccct
     1081 aaatgatgaa gcggacaaac atcactacat tatcagtact gagttagaac atttatctat
     1141 tcactacaaa tcgtcaatag agtttacaga taagtcaagt attagggaac attttttaaa
     1201 ccaattatct aaaagccact caagagacat ctttaagaaa aacacaagca tcggacctca
     1261 tcgtgatgat attactttct ttattaatga tatcaatgct acttttgcta gccaagggca
     1321 acaaagaagc cttattttat cattgaaatt agcagaaatt gagctaataa aaaccgttac
     1381 caacgattat ccgattctct tacttgacga cgtcatgagt gaacttgaca accatcgtca
     1441 actaaaactc ttagagggaa tcaaagagaa tgttcaaaca tttattacta caacaagttt
     1501 agaacattta agtgctttac ctgatcaact caaaatcttt aacgttagtg atggtactat
     1561 tagtattaat gaaaaaaaag caacagatta atctgttgct ttttaataag atttaacaat
     1621 accaagtaat atagcaccca taacaaaaca aaggataccc atgacaaccc acttctgctc
     1681 tttcttcgtt tttgtctcac ctaaaaataa aataccacca ataatagaga taattactcc
     1741 aagttgagaa aaactaaaag caattgctag ccctgcttta gctgctgcca ataacatgaa
     1801 gacattacca acgccccaca tgagacctgt aatcatattt ttaacaacaa cagcctcaaa
     1861 gttaacacga aacttcatga aacaaattgc acctagacac attccaacag ccatgggtaa
     1921 aataacggcc atagcgtcga acttcataat gttattaaat aaaactgcgt atgagatgta
     1981 acctacagtt gaataagcaa ttgtagcaaa tccttttgaa aactcttgat gtgttgccaa
     2041 agcttgttct gagacatcac gtttacttga gaaatagaag ccgataacta ataatgtcaa
     2101 cgctgtcaat cctaaaataa attggattgg cttagtccat tcatgaaaga ctaaagcacc
     2161 aactaggctt ccacctacta attgtgcacc acttgacagt ggattagcaa cagagactcc
     2221 catatatttc attgcttgga attgaccatt ttggccgact gaccatagga taccacctaa
     2281 gataccaaaa atccacaatg aggcagtcat ctctggctgt ttaaataacc atacgataat
     2341 cgcaaatagc aatgctccta aagtcattcc aaatgtttgt tgatttggac gtcctccaat
     2401 tttattacta acaaatccaa tacttcccca cgcaaacatg ggaatcaatg caataagtaa
     2461 accttccaat tctttctcct tacaatggaa tattaagtat tacctatttt caatcctaga
     2521 gaatctattg actaacttta ttagcaagat cagcaaatac tatagcttta tcaacactat
     2581 ctttttccaa agctataatg acttgtttta tcaataaatt gaccagtgat ttagaataag
     2641 gtaactgttc tttagtcata ttactaatct tatttaaaat aacatcttca ggtaaaatat
     2701 cttgaaaata gcagatgact ttgaaataaa aatcattgta ctgtgtcaca ccattcagat
     2761 aagtaactac caggttaatc tcatcttgag gcagcctcat atttaaccct aaatttgaaa
     2821 taaaaagttt agccaatatt ttgagaacat tgctttctat tctatcacag gattgttgtt
     2881 tttggaaact gaaaattctc atgatatctt cattatctcg ataagattcc agtaaagtta
     2941 attgctctaa atcagtatta gaagttccag ccttaacttt tgaatctaga atggatactt
     3001 cattgatagt taccccaacc tccaacgttt gtaaagcttt aataaattta tcaataccta
     3061 tttgtgtctc accattttca aaacgtgata gagttgattt tgataattga gcacttttgg
     3121 ctagagaact tagggatata cctttgtcct ctctcaactc ttttaaaatt tttccataat
     3181 ttttcataca aaaactttac tatatttgtt tcttttatgc aacaaaaagc atcctcatag
     3241 gggatgcttt ttgcccttat tttagtgtac tgaataattt ggtgcctcat ttgtaatttg
     3301 aacatcatgg ggatggcttt ctttcaagcc agctcctgac atttcaacaa attgagcatt
     3361 atcatgaagt tcttttatgt tcgctgcacc tacatatccc ataccagagc gaatgccacc
     3421 aagcatttgg aaaacaatat cggctactga acctttgtat gctacacgac cttcaatacc
     3481 ttctggaacc aatttgttag cctcgtttac agaaccttgg aaataacgat cgcttgaacc
     3541 ttttttcatt gctgcaattg agcccatacc acggtatgtc ttgaacttac gcccttggaa
     3601 aatttctgtt tctcctggcg cttcatctgt tcctgcaaac attgaaccta gcataactgc
     3661 atttccacct gctgcaagtg cttttacaat atcacctgaa tatttaatac caccatcagc
     3721 aatgatagtc ttaccgtact cacgcgcaac agccgctgca tcgtaaatag ctgtaatctg
     3781 aggaacacct accccagcaa cgacacgagt cgtacaaatt gaacctggac caataccaac
     3841 tttaacgaca tccacacctg catcataaag tgcacgcgct ccttctgctg tagcaatatt
     3901 tcccgcaatc aatgtgcgat tagggaagtg agcacgaatt tcagcaattt tacggagcac
     3961 accagctgaa tgtccatgtg ctgtatcaat gacaatagca tctgcacctg cttcaaacaa
     4021 agcttctgca cgttcaaaag tatcagaggt tacacctact gcacctgcaa ctaagagacg
     4081 tccaaattca tcttttgctg ccttaggaaa ttcaataact ttttcgatat ccttaatagt
     4141 aataagtcct gaaagacgtc cctcatcatc tactaaaggt agcttttcaa tacgatgctc
     4201 atgaagaata cgctctgcag tttcaagatc tgtaccaata ggagctgtta caagattttg
     4261 acttgtcatg tgctctgaga ttagttgttt ataatcagaa atgaaacgca tatcacggtt
     4321 agtaataatt ccaactaatt tacgattctc gagtgtctct acaataggaa ctccactaat
     4381 gcgatagttt tgcatcaatt cctcagcttc agagacagta ttatctggtg tcaagaagaa
     4441 tgggtcaata ataacaccat tttctgagcg tttaacttta cgaacttctt ctgcttgatc
     4501 aactattgac atatttttat gaatgatacc aagcccacca gcacgtgcaa tagcaatagc
     4561 catcttacta tctgtaactg tatccattgc agctgtaatg attggaatgt ttaatgtcaa
     4621 attatcagcc aactttgtct tcatatcaac ttcatttggt aaaacatggc tttcagcagg
     4681 aataagcaga acatcatcaa aagtaaaacc ttttttcaaa aacttagtat cccaatttga
     4741 catcaaaaaa tcctcttttc ttagatagta gatataaaaa atcccattaa aattatattg
     4801 ttactatcat atcacgctaa aatcatttgt caatataaaa agagcataga attaatagct
     4861 tctagctctt taaagtaatg aaaaaatctt cttaattttc tatttttttg agaagaagaa
     4921 aggcggcgta tactgactta atttctcaaa acacttttga gcttcttcac tattattaca
     4981 aataatcaaa caagtatcat caccacagac agttgctaag atctcaggta ataaaatcga
     5041 atctaatatg gagccaaaag attgagctaa gccaggtagt gtcttcaaaa cgatttggtt
     5101 ttgaactgga aacagcatga ctagggcatc ttccatgtaa aaacgtaacc gttcttccca
     5161 acgcttctga gatatactat gaatctcata ataagtttct gtagcattat cactgctttc
     5221 gttaacctta attaaattaa gttccttcat atctcgagat aatgttgctt gcgtcactga
     5281 aacgccattt ttttctagta attcccttaa ctcatgttgt gtacgcacct tagtctcaga
     5341 gactaaggaa cgaattaatt ggtgtcttgt ttctttttta ttcatataaa ctccttattc
     5401 aatgaatttc ttgaaataat ttttattatt atagagaagc ttctttcctt tcatttcaac
     5461 gatatctttt tggcgtaatt cctttaaaac atgactaact gtctcgcgag tagttccgct
     5521 catattggca atttcaatcg ttgtaatagg aaaaggtaaa tgtccccttt ctgtaggaac
     5581 ttgcaaaagt agagttgcaa gcgattggat aactctcata ctggcacttg aagtcaccat
     5641 attacgaagt cgtatttcat gaactcgtaa tagcttagaa tatttccgac aaagatgctt
     5701 catttgattg atattttgaa gagagtattc ttcgtataaa gccattggca gagaaaaata
     5761 ctcaatatcc gtaatagcca ctgctgaaaa atggtaatca tcatccaaaa agagacctcc
     5821 ataaggaaaa accgtacctt gtctaacata atctgtataa atgaaagaac cagaagcgtc
     5881 tgattgttca atttttacat gtcctgactg gattaaaaac agtttttcac ggtaatcacc
     5941 ttcaaaaaat aaggtatgat tttttagagc tgtacgatgt ttaatgtggc tcacaatatg
     6001 gtcaaattgt tcaatagtaa agtgtttaaa atcatctagt tgtctgaaat aaaaatattg
     6061 ttcttttgta atcacctgat tttctagacc tttctaatca ttagatgcaa acattttttg
     6121 tataatgaaa attttttaat gtataaaatt ttcttgtcac gataaatcct ccttcattat
     6181 agcaaaaaaa ttcgtagaaa gaacgttttt cgttgaattt tgaaaacgct tttgagaaaa
     6241 tgtaattgtt ctgatatctt ttaaaaaaat tgagaggcct tcatgactaa atttattgtt
     6301 gattcatcgt actggaattt gtttccaact tctaaaattg gagtaatctt aatcaaagat
     6361 tatcacatgg acagaaacct tgaaacagaa cttaaacagt tactgtctga tagtcattct
     6421 ttagcaaaaa aatatttgca agagaaagaa ttcagtcaaa acagagttat ccaaacttat
     6481 cgcaaagcat atcaaacctt taaaactaaa aaaggtgcac gttcaagtat cgaagcatta
     6541 ttgaagcgag ttaattcagg aaacgaaatt actagtataa atcctctagt tgatatctat
     6601 aatgccgcta gccttaggtt tggattacct attggtgctg aggatagtga tacatttaga
     6661 ggtgatttaa aactaactat taccaatggt ggtgatgaat tttacttgat tggcgaagat
     6721 tttaacagac caactctttc aggtgagctt gcctatgttg atgatgttgg agcagtttgc
     6781 cgctgtttta attggcgtga tggaaaacgt actatgatta ccgataatac tcagaatgct
     6841 tttttagtta tcgaattaat tgataatgga agagaaatta tttttaaaga agcgttggac
     6901 tttatcgcca ccaatacaaa tcgttttcta aaagctaaaa cacagaccat catattagat
     6961 aaagaacaca gtgaaataac gctataacat taaaacgaaa agtaaaaatt ttacttttcg
     7021 ttattttttt aggttaataa tttctcactt tccaacgata aaagaaagaa attgtataga
     7081 aatcgctttc atttctataa atttaaattt ttttggatat aatcgtgaca ttcttaacac
     7141 ttttaatgcg aataacttcc ctattttata ttcaaaagaa agtagaatgt aaatgtagat
     7201 atagagataa atcctgtttc aatacaaatc tctaactaag aaaggagtaa aatatgactc
     7261 aaacacatcc aattcatgtt ttctcagaaa ttggaaaact taaaaaagtt atgctacacc
     7321 gtccaggtaa agaaatagaa aatctgatgc ctgattattt agagagactc ttgtttgatg
     7381 atatcccatt tttggaagat gcacaaaaag aacacgatgc gtttgcacaa gcacttcgca
     7441 acgaaggagt cgaagtactt taccttgaaa atcttgctgc cgagtcgtta acaaaccaag
     7501 aaattcgtga acagtttatc gatgagtaca ttggcgaagc aaatgttcgc ggtcgtgcta
     7561 ctaaaaaagc tattcgagaa ttattactca atatcaaaga taataaagaa cttatcgaaa
     7621 aaactatggc aggtatccaa aagtctgagc tacctgaaat cccatcttct gaaaaaggtc
     7681 taactgatct tgtcgaatct aattatccat ttgccataga tccgatgcca aatctttact
     7741 ttacacgtga tccatttgca actattggga atggagtttc attaaatcat atgttctctg
     7801 aaacacgtaa tcgcgaaact ttatacggta aatatatctt tacacatcac ccagagtacg
     7861 gtggaaaagt ccctatggtc tatgaacgtg aagaaacaac tcgtattgaa ggtggagatg
     7921 agcttgtcct ttctaaagat gtccttgccg ttggtatctc tcaacgtact gatgctgcct
     7981 ctattgaaaa actcttagtc aatattttta aacaaaattt aggttttaaa aaggttttag
     8041 catttgaatt tgcaaataat cgtaaattta tgcaccttga cacagtcttt acaatggttg
     8101 actatgacaa gtttacaatt cacccagaaa ttgaaggcga tcttcgcgtt tactctgtaa
     8161 cttacgaaaa ccaagatctt catattgaag aagaaaaagg tgatttggca gatcttctag
     8221 cgaaaaacct aggagttgag aaagtggaat tgattcgttg tgggggtgac aacttagtcg
     8281 ctgctggacg tgaacaatgg aatgatggtt caaatacatt aactatagca ccaggtgtcg
     8341 ttatcgttta caacagaaat acaatcacaa atgcaatctt agagtccaaa ggattgaaat
     8401 taattaagat taatggaagt gaattagttc gtgggcgtgg tggaccacgc tgcatgtcaa
     8461 tgccatttga acgtgaagac ctttaaaatt tatgaagcta ttagtcaatc acttgcctta
     8521 ccaaagagct gcaagcctat atattcgctt ttctgttttc gttatcgaaa gaaatataaa
     8581 aatggaagaa gaattcgatg ataatgacga gcaagatact atttatgctg ttctttatga
     8641 tgggaaacag ccagtttcaa caggacgctt tcttcctgaa actcaaacag aagctcgttt
     8701 gactcgaatt gcgactttaa aaggttatcg tggtaacggc tatggtacca aaattattat
     8761 agcactagaa aattatgcaa aagaaaatgg ttatcactat ctaactatac atgctgaatt
     8821 gacagcaaaa gatttctacc agacacttgg gtatcaagct actgggaata tttatatgga
     8881 agacggggaa gcctgtcaaa cactcgaaaa atatttaatt taaaggagac attaacatat
     8941 gacacaagta tttcaaggac gtagtttctt agcagaaaaa gatttttctc gtgaggaatt
     9001 tgaatatctt attgattttt cagctcattt aaaagacctt aaaaaacgtg gtgttcctca
     9061 tcattatctt gaaggtaaaa atattgctct cttatttgaa aaaacatcta ctcgtactcg
     9121 cgcagccttt acaactgcag caattgacct aggcgctcat ccggaatacc ttggtgcaaa
     9181 tgatattcaa cttggtaaaa aagaatcaac agaagatact gctaaggttt taggacgtat
     9241 gtttgatggt attgaattcc gtggttttag ccaaagaatg gttgaagagc ttgctgaatt
     9301 ttctggagta cctgtctgga atggtttaac agatgaatgg catccaacac aaatgctagc
     9361 tgactacctt actatcaaag aaaacttcgg taaacttgaa ggtattactc ttgtttactg
     9421 tggtgacgga cgtaacaatg ttgccaactc gcttttagtg gctgggactt tgatgggggt
     9481 caatgtacac atcttttctc caaaagaact tttccctgct gaagagattg ttaaattggc
     9541 tgaagaatat gccaaagagt ccggggctca cgttctcgtt actgataatg tagacgaagc
     9601 tgtaaaggga gcagatgtct tttacactga tgtctgggta tcgatgggag aagaagataa
     9661 gttcaaagaa cgcgttgaac ttcttcaacc atatcaagta aacatggaac tgattaaaaa
     9721 agctaataat gataatctta tcttcttaca ctgcttacct gcattccatg atacaaatac
     9781 cgtttacggc aaagacgtcg ctgaaaaatt tggcgtcaag gaaatggaag ttactgatga
     9841 agtcttccgt agcaaatatg ctcgtcattt cgaccaagct gaaaatcgta tgcacactat
     9901 taaagctgta atggctgcaa cccttggaaa tcttttcatt ccaaaagttt aaattaaacg
     9961 actataggga gttgacatat agtcaattcc ctattttagg aaatgaggta taaaatggaa
    10021 aaagaaaaaa aattaggtct tttaccacta acaatgcttg tcattggctc tcttatcggt
    10081 ggcggaatct ttgatttaat gcaaaatatg agttccagag ccggtttggt accaatgctt
    10141 attgcttggg taattactgc tatcgggatg gggactttcg ttttaagttt tcaaaattta
    10201 tctgaaaaaa ggccggacct aacagctgga atctttagtt acgctaaaga ggggtttgga
    10261 aactttatgg gatttaactc tgcatggggt tattggttat cagcttggct tggaaatgtt
    10321 gcctacgctg cactcttatt cagttcactc ggttatttct ttaaattctt tggtaatgga
    10381 aataatatca tctcaattat tggagcaagt atagttattt gggttgtcca tttcttaatt
    10441 ttaagaggtg ttaatacagc tgcatttatt aataccatag ttacctttgc aaaattagta
    10501 cctgttatta ttttcttaat ttcagcgtta ttagctttca aatttaacat ttttagtctt
    10561 gatatctggg gaaatggatt acatcaatca attttcaacc aagtcaattc aactatgaaa
    10621 accgctgttt gggtatttat tggtattgag ggcgccgttg tcttctcagg tcgtgctaaa
    10681 aaacactctg atattggtaa agcaagtatc ctagcattat tcactatgat ttcactttat
    10741 gtattgattt ctgttttatc acttggtatc atgtcacgtc cagaacttgc aaacttaaaa
    10801 acaccagcta tggcttacgt tctagaaaag gctgttggtc actggggcgc tatcttagtt
    10861 aaccttggtg ttatcatttc agtatttggc gctattcttg cttggacttt atttgcagca
    10921 gaattaccat atcaagctgc taaagaaggt gcttttccta aattctttgc aaaagaaaat
    10981 aaaaacaaag ctccaatcaa ttcactctta gtcactaatc tttgtgtaca agcattctta
    11041 atcacgttct tattcacaca aagtgcttat cgttttggtt tcgcattagc atcatctgct
    11101 atcttaattc cttatgcttt tacagcacta tatcaattac aattcacact ccgtgaggat
    11161 aagtcaactc caggacatca aaagaattta attatcggta tcctcgctac aatctatgct
    11221 gtttacctta tctacgctgg tggttttgat tacttacttt tgacaatgat tgcttacact
    11281 ctaggtatga ttctctatat taaaatgaga aaagatgaca agcttcctat ctttgtggga
    11341 tatgaaaaaa ttagtgccat tgtcattcta gcgctttgct tattatgtat tattgaaatt
    11401 atgactggac aaattgacat tgctgcaatc ctagcaggtt agtcattaga aagagggata
    11461 aattggtaaa agaacatcaa aaaatcgtag ttgccttagg tggcaacgct attctatcaa
    11521 cagatgcttc agcaaaagca caacaagaag cattaatcaa cacatcaaaa tcactggtca
    11581 aactgattaa agaaggccat gatgtcattg ttactcatgg aaatggtccc caagttggta
    11641 acttattgct acagcaagca gcttcagatt ctgaaaagaa tccagcaatg cctttggata
    11701 cttgtgttgc catgacagaa ggaagtattg gtttttggtt acaaaatgct cttaacaatg
    11761 aattacaaga acaaggcatt gacaaagagg ttgcaacagt ggtaactcaa gttattgtag
    11821 atgaaaaaga tcaagctttc actaatccaa caaaacctat tggcccattc ttatctgaag
    11881 aagatgccaa gaaacaagcc caagaaacag gttcaaaatt taaagaagat gctggccgtg
    11941 gatggcgtaa agtcgttcct tcaccaaaac cggttggtat caaagaagct tctgttatta
    12001 gacggttagt ggactctggt gtggttgtta ttagcgcagg tggcggaggt gtaccagtta
    12061 ttgaagatgc taatacaaag gcattaaaag gggttgaagc tgttattgac aaagactttg
    12121 ccagtcaaac tttatcagaa cttgttgatg ctgacttatt catcgtctta actggcgttg
    12181 acaacgtttt tgttaatttt aataaaccta atcaagaaaa attagaagaa gttactgttt
    12241 cacaaatgaa acaatatatc actgaaaatc aatttgcccc tggaagtatg ctaccaaagg
    12301 tcgaagctgc gattgctttt gttgaaaata aacctgaatc acgagctatt attacttcac
    12361 ttgaaaatat tgataatgtt ttagcccaaa atgcagggac acaaattgtt gcaaattaaa
    12421 caatgtagat taccattaca tacactcctt attaatgtca c
//
LOCUS       DATRTN010000051        12337 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.051, whole genome shotgun sequence.
ACCESSION   DATRTN010000051 DATRTN010000000
VERSION     DATRTN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12337
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.051"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(6..1031)
                     /gene="trpS"
                     /locus_tag="VBE22_001344"
     CDS             complement(6..1031)
                     /gene="trpS"
                     /locus_tag="VBE22_001344"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEO7202806.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQNEGSYTLFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTLFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPAGFLVYPVAQAADITAFKANLVPVGTDQKPMIE
                     QTREIVRSFNHAYNCQVLVEPEGIYPENDAAGRLPGLDGNAKMSKSLNNGIFLADDMD
                     TVKKKVMSMYTDPNHIKVEEPGQIEGNMVFHYLDVFGRDEDQKEITAMKEHYQKGGLG
                     DVKTKRYLLDILERELSPIRERRLEYAKDMGQVYQMLQKGSEKAQAVAASTLDEVKSA
                     MGLNYFK"
     gene            complement(1139..1831)
                     /locus_tag="VBE22_001345"
     CDS             complement(1139..1831)
                     /locus_tag="VBE22_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO7202807.1"
                     /translation="MRRDDIIMPQDGRKMAVLLGFIGGFIDVYSHMQFHSLVATQTGN
                     IILMVANLGQNDFHFFITKLFSIFFFSIGFLVGIVIKERAVTAFWRLYALCPLLLSTM
                     FIPFVENVNHNIWVALLAFSTGILMLTFSGSRIESEPYTILMTSGNYRKMLQFWYEYI
                     VNRQRTAMQKRRAINYSLVVLAFIIGALVAAIVYDIFAYRAILGVTITLLIIMIHYTI
                     EIIKNDLTLHNV"
     gene            2075..2947
                     /locus_tag="VBE22_001346"
     CDS             2075..2947
                     /locus_tag="VBE22_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO7202808.1"
                     /translation="MSKKIKNFILLNIGSLIVAFGFNAFFLHNHIVTGGISGLAVSFH
                     EVFGWDSAFFIMIINIPLLLLCYFGLGKQTFLKTVYGSWIFPVFIKLTQSVPTLTHNP
                     LLAALFGGVIVGCGLGIVFWSDSSTGGTGIIIQFLGKYTPISLGQGVILIDGLVTIVG
                     FLAFDSDTVMFSIIGLITISYIINAIQTGFTTLSTVLIVSQEHQKIKTYINTVADRGV
                     TEIPVKGGYSGTNQIMLMTTIAGYEFAKLQEAIAEIDETAFITVTPTSQASGRGFSLQ
                     KNHGRLDEDILMPM"
     gene            3013..4632
                     /locus_tag="VBE22_001347"
     CDS             3013..4632
                     /locus_tag="VBE22_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7202809.1"
                     /translation="MLTVSDVSLRFSDRKLFDEVNINFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHIALGPDERLSVLRQNHFDYEDERVIDVVIMGNETLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEELHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKESSELAARLQADRNAKAEEKIKQLQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFVNFKAEREMGNDLLTVENLSVTIDGEKI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSRSYLPKDNSR
                     DFASGESILEWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKDFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENTEVQAKVAQLWK"
     gene            4755..7334
                     /locus_tag="VBE22_001348"
     CDS             4755..7334
                     /locus_tag="VBE22_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEO7202810.1"
                     /translation="MTINHYKLRIPYYTISFLLPFIIIVCILFTKNIYWGSPTTILAS
                     DGFHQYVIFNQALRNILHGSNSLFYTFTSGLGLNFYALSSYYLGSFLSPIVYFFNLKN
                     MPDAIYLLTICKIGLIGLSMFVTLCKRHCKVNRVLLLVISTCYSLMSFSISQIEINMW
                     LDVFILIPLVVLGVDQLLWERKPILYFLSLTALFIQNYYFGFMTAIFTSLYFIVQITR
                     NTDSKVAFKQFLHFTFLSLLAGMTSSIMILPTYFDLTTHGEKLTKVSKMFTENSWYMD
                     LFAKNMIGAYDTTKFGSIPMIYVGLLPLLLSLLYFTIKEVPRRTRLAYGFLIIFVIAS
                     FYITPLDLFWQGMHAPNMFLHRYSWVLSVLICLLAAECLEYLDNISWKKILGVNLILV
                     SGFIITFLFKKHYHYLNLELLLLTLTFLSAYIILTISFVSKQIPKLVFYPFLIGFVVL
                     EMTLNTFYQLNSLNDEWIFPSRQGYAKYNHSISKLVRKTERNNSTFFRTERWLGQTGN
                     DSMKYNYNGISQFSSIRNRSSSQVLDRLGFKSDGTNLNLRYQNNTLIADSLFGVKYNL
                     TEYPFDKFGFIKKAQDKQTILYKNQFASQLAILTNQVYQDKPFTVNTLDNQTTLLNQL
                     SGLKETYFEHLIPNSVSGQTTLNKQVFVKKNKQGNTEITYNITIPKNSQLYVSMPFIN
                     FNNEENKIVQISVNNGPFVPNTLDNAYSFFNIGSFAENSRIKVKFQFPHNDQVSFPIP
                     HFYGLKLEAYQKAMTVINKRKVKVRTDHNKVIANYTSPNRSSLFFTIPYDRGWKAYQN
                     NKEIKIFKAQKGFMKINIPKGKGKVTLIFIPYGFKFGVGLSITGIVLFTVYYFKFGKN
                     KIG"
     gene            complement(7402..7475)
                     /locus_tag="VBE22_001349"
     tRNA            complement(7402..7475)
                     /locus_tag="VBE22_001349"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7439..7441),aa:Asn,seq:gtt)
     gene            complement(7505..7576)
                     /locus_tag="VBE22_001350"
     tRNA            complement(7505..7576)
                     /locus_tag="VBE22_001350"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7541..7543),aa:Glu,seq:ttc)
     gene            7768..7841
                     /locus_tag="VBE22_001351"
     tRNA            7768..7841
                     /locus_tag="VBE22_001351"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7802..7804,aa:Arg,seq:tct)
     gene            complement(7913..8392)
                     /gene="rlmH"
                     /locus_tag="VBE22_001352"
     CDS             complement(7913..8392)
                     /gene="rlmH"
                     /locus_tag="VBE22_001352"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEO7202811.1"
                     /translation="MKLKIITVGKLKEKYLKEGVAEYQKRLNRFSKIETIELADEKTP
                     DKASISENQRILDIEGERILSKIGERDYVIGLAIEGKQLPSESFSHLIDQKMISGYST
                     ITFVIGGSLGLSQKVKKRADYLMSFGLLTLPHQLMKLVLMEQIYRAFMIRQGTPYHK"
     gene            8593..9822
                     /locus_tag="VBE22_001353"
     CDS             8593..9822
                     /locus_tag="VBE22_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEO7202812.1"
                     /translation="MKKKLVSSLLKCSLIIIVSFAGGAFASFVMNHNDNIPNGGVTKT
                     SKVNYNNITPTTKAVKKVQNSVVSVINYKQQESRSDLSDFYSHFFGNQGGNTDKGLQV
                     YGEGSGVIYKKDGKNAYVVTNNHVIDGAKQIEIQLADGSKAVGKLVGSDTYSDLAVVK
                     IPSDKVSNIAEFADSSKLNIGETAIAIGSPLGTEYANSVTQGIVSSLKRTVTMTNEEG
                     QTVSTNAIQTDAAINPGNSGGALINIEGQVIGINSSKISSTSNQTSGQSSGNSVEGMG
                     FAIPSNDVVKIINQLESNGQVERPALGISMAGLSNLPSDVISKLKIPSNVTNGIVVAS
                     IQSGMPAQGKLKKYDVITKVDDKEVVSPSDLQSLLYGHQVGDSITVTFYRGENKQTVT
                     IKLTKTSKDLAKQRANN"
     gene            9920..10693
                     /locus_tag="VBE22_001354"
     CDS             9920..10693
                     /locus_tag="VBE22_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEO7202813.1"
                     /translation="MEYLETININHIAPNPYQPRLEFNTKELEELANSIKINGLIQPI
                     IVRPSAVFGYELVAGERRLRAAKLAKLESIPAIIKSYNNDDSMQLAIVENLQRSNLSP
                     IEEAKAYSQLLQKKSMTHEELAKYMGKSRPYISNTIRLLNLPPLITSAIEEGKLSSGH
                     ARALLSLPDASQQKDWYQRILTEDISVRRLEKLLKQEKKTNHKSLQNKDVFLKHQENE
                     LAQFLGSKVKLTINKDGAGNIKIAFANQEELNRIINTLK"
     gene            10892..12253
                     /gene="dnaA"
                     /locus_tag="VBE22_001355"
     CDS             10892..12253
                     /gene="dnaA"
                     /locus_tag="VBE22_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEO7202814.1"
                     /translation="MTENEQLFWNRVLELSRSQIAPAAYEFFVLEARLLKIEHQTAVI
                     TLDNIEMKKLFWEQNLGPVILTAGFEIFNAEITANYVSNDLHLQETSFSNYQQSSNEV
                     NTLPIRKIDSNLKEKYTFANFVQGDENRWAVSASIAVADSPGTTYNPLFIWGGPGLGK
                     THLLNAIGNQVLRDNPNARVLYITAENFINEFVSHIRLDSMEELKEKFRNLDLLLIDD
                     IQSLAKKTLGGTQEEFFNTFNALHTNDKQIVLTSDRNPNQLNDLEERLVTRFSWGLPV
                     NITPPDFETRVAILTNKIQEYPYDFPQDTIEYLAGEFDSNVRELEGALKNISLVADFK
                     HAKTITVDIAAEAIRARKNDGPIVTVIPIEEIQIQVGKFYGVTVKEIKATKRTQDIVL
                     ARQVAMYLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMVAQDDNLRIEIETIKN
                     KIR"
BASE COUNT         4160 a         2108 c         2006 g         4063 t
ORIGIN      
        1 atatattatt taaaatagtt caaacccatg gctgatttaa cctcatccaa agtagaggct
       61 gcaacagctt gagctttctc actacctttt tgtaacattt gataaacttg tcccatatct
      121 ttagcatatt cgaggcgacg ttctctaata ggtgataatt cacgctctaa gatatcaagt
      181 agataacgtt ttgttttaac atccccaagt ccaccttttt gatagtgttc tttcatagca
      241 gtaatctctt tttggtcttc atctcttcca aaaacatcaa ggtaatgaaa taccatatta
      301 ccttcgattt gtcctggttc ttctacttta atgtggtttg gatcagtata catactcata
      361 acttttttct taactgtatc catatcatct gctaaaaaga ttccattatt taatgactta
      421 gacatttttg cattaccatc taaccctggc agacgacctg ctgcatcatt ttctggatag
      481 attccctctg gttcaactaa aacctgacaa ttataagcat gattgaaaga tctaactatt
      541 tcacgtgttt gttcaatcat tggtttttgg tcagtcccta caggaacaag gttagcttta
      601 aaagcggtta tatcagctgc ttgtgcaaca ggataaacta agaatcctgc tggaatagac
      661 tcaccaaaac ctttttgagc aatttctgtt tttacagttg gattacgttc taaacgtgca
      721 agtgaaacca agttcatata atacatactt aattctgcta actcaggaat ttgactttga
      781 atgaataaag tcgacttgtt tggatctaac ccaacagcta aataatcaag tgcaacatta
      841 ccaattgatt caacaatagt ttgcggatct ttagcatggt ctgtcagtgc ttgttggtct
      901 gctaaaaaga caaataaagt atagctaccc tcattttgaa gtagaactct attttttaag
      961 ctacctacat aatgtccaat atgcaacttt cctgtcggtc tatcacctgt taagataatt
     1021 ggttttgtca tgtttactcc ttaatatacc aaagatatca tttcatatag ttatcttatc
     1081 atgtcacaag ccctattgca attcatccct acaagaaaca ttcaccttat taagatgatt
     1141 aaacattatg taacgttaaa tcgttcttaa taatttcaat cgtataatga atcataataa
     1201 ttaaaagcgt tatcgtcaca cctaaaatag ctctatatgc aaaaatatcg taaactattg
     1261 cagctaccaa tgctccaata ataaatgcta gaactaccag tgaataattg atagctcgtc
     1321 ttttttgcat agccgttctc tgacgattaa caatatactc ataccaaaat tgtagcattt
     1381 tccgataatt accagaagtc attaagatag tatatggttc cgattcaatg cgacttcctg
     1441 aaaatgtcaa cattaaaata cctgtgctaa aggctaataa agctacccag atattgtggt
     1501 ttacattttc aacaaaagga ataaacatcg tagaaagtaa aaggggacat aatgcatata
     1561 gacgccaaaa agctgttact gcacgttctt taatcacaat accaaccaaa aaaccaattg
     1621 aaaaaaagaa aatagaaaat aatttagtga taaaaaagtg aaaatcattt tgtccgagat
     1681 ttgccaccat caagataata ttacctgtct gtgttgcaac aagggagtga aactgcatgt
     1741 gactataaac gtcaataaaa ccaccaataa aacccaataa tacagccatt tttcttccat
     1801 cttgtggcat tataatatca tctctgcgca ttatagcaaa ttctccttct tgtattatca
     1861 tgttttctat tatataccaa taaaataggc attcaactca aaaatatcga gaaatttctt
     1921 ttctaaattt acttgactac caagtctcgc tgttagataa taggtaataa taagttttga
     1981 taaaataaaa atgttatgac tgagttgtca atattattta ttgacagctt tctcttttta
     2041 tcatgttatt attactaaag ttaaggagct tatcatgtct aaaaaaatta agaattttat
     2101 actattaaat attggatctc taattgttgc atttggtttc aacgctttct ttcttcataa
     2161 tcatattgtc acggggggga ttagtggttt ggcagttagt tttcatgaag tatttggttg
     2221 ggattctgct ttttttatta tgattatcaa tattccattg ctccttcttt gctactttgg
     2281 cttaggtaaa caaacctttt taaaaactgt ctatggttct tggatttttc ctgtttttat
     2341 taagttaaca caaagtgtac caactttgac ccacaaccca ctcctcgcag cactttttgg
     2401 aggtgttatt gtaggatgtg gtttggggat tgttttttgg agcgactctt caactggtgg
     2461 aacggggatt atcattcaat tcttaggaaa atatactcct ataagccttg gacaaggggt
     2521 tatattgatt gatggacttg ttacaattgt tggtttccta gcttttgaca gtgatacggt
     2581 tatgttttct attattgggt tgataactat tagttatatt attaatgcta tccaaactgg
     2641 atttacaacc ttaagcactg tcttaatcgt ttctcaagag caccaaaaaa ttaagacata
     2701 tatcaatact gtcgcagata gaggagtaac agaaattccc gttaaagggg gatattctgg
     2761 aactaatcaa atcatgctta tgacaactat tgctggttat gagtttgcta aattacaaga
     2821 ggcaatagca gaaattgacg aaacagcctt cataacagta actccaacat cacaagcttc
     2881 tggacgtgga tttagtcttc aaaaaaatca tggacgtctt gatgaagaca ttcttatgcc
     2941 aatgtaatcg attgataatt aaagcttttt ctagtagaat agcagataga atatacacaa
     3001 gaggtaacaa aattgcttac agtttcagat gtttcactac gttttagtga tcgcaaatta
     3061 tttgatgaag ttaacattaa ctttacagca ggaaatacct atggtcttat cggagcaaat
     3121 ggcgctggta aatcaacttt ccttaaaatt ttagctggtg atattgaacc aacaacagga
     3181 catattgcac ttggcccaga tgaacgcctt tctgtacttc gccaaaatca ttttgattat
     3241 gaagatgagc gcgtcattga tgtcgttatc atgggtaatg aaacacttta tagtatcatg
     3301 aaagaaaagg atgctatcta catgaaagaa gatttttctg atgaagatgg tgttcgcgct
     3361 gcggaattag aaggagaatt tgctgaactc ggtggatggg aggctgaaag tgaggcttct
     3421 caattactcc aaaatctaaa tatttcagaa gagcttcatt atcaaaatat gagtgaacta
     3481 gctaatggag acaaagttaa agttctcctt gcaaaagctc tctttggtaa accagatgta
     3541 cttctccttg acgagcctac caatggactt gatatccagt ctattacttg gttagaagat
     3601 ttcttgattg actttgaaaa tacagttatt gtagtttccc acgaccgtca tttcttgaat
     3661 aaagtatgta cacatatggc tgaccttgat tttggaaaaa ttaaactgtt cgtcggtaac
     3721 tatgatttct ggaaagaatc atcagaatta gcagctcgtt tacaagctga tcgtaatgct
     3781 aaagcagaag aaaaaattaa acaacttcaa gaattcgttg cacgattttc agcgaatgct
     3841 tcaaaatcaa aacaagcaac ttctcgtaaa aaaatgcttg ataaaattga acttgaagag
     3901 attgtaccat caagccgtaa atacccattc gttaacttta aagccgaacg cgaaatggga
     3961 aatgatttat tgaccgttga aaatctctct gtcactattg atggggaaaa aatccttgat
     4021 aatatcagtt tcattcttcg tcctggtgat aaaactgcac ttattggaca aaatgatatc
     4081 caaactactg cacttattcg agcattgatg ggggatatcg aatatgaagg aacaatcaaa
     4141 tggggagtaa caactagtcg ttcttacctt ccaaaagaca attcacgaga ttttgcttct
     4201 ggcgagtcaa tcttagaatg gcttcgtcaa tttgctagta aagaagaaga cgataatacc
     4261 ttcttacgtg gtttcctagg tcgtatgctt ttctctggag atgaggttaa caaatctgtt
     4321 aatgtcttat ctgggggtga aaaagtacgc gttatgcttt cgaaattaat gctattaaaa
     4381 tctaatgttc ttgttcttga tgatccaaca aaccacttag atttggaatc tatttcaagt
     4441 ctcaacgatg gactaaaaga tttcaaagaa tctattattt ttgcaagtca tgaccacgag
     4501 tttattcaaa ctttagcgaa ccatattatt gttctttcta aaaacggagt tatcgatcgt
     4561 atcgatgaaa cttacgatga atttcttgaa aataccgaag tacaagctaa ggtcgctcaa
     4621 ctctggaaat aatcctttgg catatctcta acctaataaa cgtgtcaaga ttgaaaatct
     4681 taaaactata ttaatcattt aaataaaaag gttggaacaa atagtgtcca gcctttttat
     4741 cttaggagat atatatgaca attaaccact ataagcttcg tataccttac tatacaataa
     4801 gctttctact tcccttcata atcatagtct gtatcctctt tactaagaat atttattggg
     4861 gaagtccaac aaccatatta gcaagcgatg gctttcatca atatgttatc tttaatcaag
     4921 cattaagaaa tattcttcat ggttcaaaca gcttattcta tacctttaca agcggtttag
     4981 gtttaaattt ttatgctctg agtagttact atttgggaag ttttttatcc ccaatagttt
     5041 atttctttaa cctaaaaaat atgcctgatg ctatctacct tttaacaata tgtaaaatag
     5101 ggttgatagg tttgtcaatg tttgtcactc tttgtaaacg tcattgtaaa gttaatcgtg
     5161 tcttactttt agtaatttct acttgctata gcttaatgag tttctcaatt agccaaattg
     5221 aaattaatat gtggctagat gtatttattc taattcccct tgttgtttta ggagttgatc
     5281 agctactatg ggaaagaaaa cctatacttt atttcctctc tctgacagcc ttattcatcc
     5341 aaaactatta ttttggtttt atgactgcca tctttactag cctttacttc atcgtacaaa
     5401 taacccgtaa tactgatagt aaggttgcat ttaaacaatt tcttcatttt acctttttat
     5461 ctcttttagc tggtatgact agtagcatta tgattttgcc aacttatttt gatttaacta
     5521 ctcatggtga aaagttgaca aaggtatcga agatgtttac agaaaattct tggtacatgg
     5581 atttgtttgc taaaaatatg attggagcct atgatacaac aaaatttggc tctattccta
     5641 tgatttatgt aggtttactt cctttactcc tttctttact ttactttaca ataaaggaag
     5701 ttcctcgaag gactagatta gcatatggat ttttaataat atttgttatt gctagttttt
     5761 atataacacc tctagattta ttttggcaag ggatgcatgc tccaaatatg tttctccatc
     5821 gctactcttg ggtactttca gtacttatct gtctgctagc tgctgaatgc cttgagtatt
     5881 tagacaacat ttcttggaaa aagattttag gggtcaattt aattttagta agtggtttca
     5941 taatcacgtt tttatttaaa aagcattatc attaccttaa tcttgaatta cttcttttaa
     6001 ccttaacttt cctctcagct tatattattc taactattag ttttgtcagt aagcaaatcc
     6061 ctaaattagt tttttatccc tttcttattg gttttgttgt attagagatg acattaaaca
     6121 ccttttacca actaaatagt cttaacgatg aatggatatt tccatctagg caaggatatg
     6181 ctaaatataa tcacagcatt agcaaacttg tcagaaagac tgagagaaat aactctacat
     6241 tcttccgtac agagcgctgg ttaggacaaa ccggcaacga ctctatgaaa tataattata
     6301 atggtatttc acaattttca tctattagaa atcgttcttc aagtcaagtg cttgatagac
     6361 ttggctttaa atcagatggt acaaatttaa atttacgtta tcaaaataat actttgatcg
     6421 cagatagtct atttggtgtg aagtataacc ttacagaata tccttttgat aaatttggtt
     6481 ttatcaaaaa agctcaagat aaacaaacca ttctttacaa aaatcagttt gccagtcaat
     6541 tagctatact aacgaatcaa gtatatcaag ataaaccatt tactgtgaat actttggata
     6601 atcagacaac tcttttaaat caattaagtg gtttgaaaga gacgtatttt gagcatctta
     6661 ttccaaatag cgtttctggg caaaccactc ttaataaaca agtatttgtt aaaaagaata
     6721 aacaagggaa tactgaaatt acctataaca taactatccc taaaaacagt cagttatatg
     6781 ttagtatgcc gtttataaat tttaataatg aggaaaataa aattgttcaa atctccgtta
     6841 ataatggacc atttgtacct aatactcttg ataatgctta ttcatttttt aatattggtt
     6901 cctttgctga aaacagtcgt atcaaagtaa aatttcaatt tccacacaat gatcaagttt
     6961 ctttcccaat tcctcacttt tatgggctca agttagaagc ttatcaaaaa gctatgactg
     7021 ttattaataa acgaaaagta aaagtaagaa ctgatcacaa taaagttatt gctaattata
     7081 caagtcctaa tagaagttct cttttcttca ctattcccta tgataggggt tggaaagctt
     7141 atcaaaataa taaagaaata aaaattttca aagctcagaa aggttttatg aaaataaata
     7201 ttcctaaagg aaaaggcaaa gtaacattaa tttttatccc ttacggcttt aaatttgggg
     7261 taggcctatc tattactgga atagtattat ttactgtcta ttattttaaa tttggaaaaa
     7321 ataaaatagg atgagagtat tctcatccta ttttattaac aaaaaaacgc cgacaaatca
     7381 acgtttagat gtgtcttatc actccggcag caggactcga acctgcgaca tcatgattaa
     7441 cagtcatgcg ctactaccaa ctgagctatg ccggataaat aaaaaactgc aaagcagtta
     7501 tcaatagtcc gtacgggatt cgaacccgtg ttaccgccgt gaaaaggcgg tgtcttaacc
     7561 ccttgaccaa cggaccatag gttaggtgtt tccttagtac tcttaatatt atatcagaaa
     7621 aaaaaggttt gtctatactt ttgttcaata atttttacaa aaaaattgaa cctacttatg
     7681 agcaaaaaaa tatatttcca actcataaat agtacatatg aaaacttgac agcctgccta
     7741 atattttggt agaataacag agttgatggt tccatagctc agctggatag agcattcgcc
     7801 ttctaagcga acggtcgcag gttcgaatcc tgctggaatc agatattgaa agcctagata
     7861 gattatctag gctttttatt tgtttttagc agttgtttca taaatggaac gattatttgt
     7921 ggtaaggagt gccttgacga atcataaaag cacgataaat ttgttccatc aagactaatt
     7981 tcataagttg atgaggtaag gttaacagtc caaaactcat taaataatca gcacgttttt
     8041 tcactttttg agataaaccc aaactaccac caataacaaa agtaatagta gagtagccgg
     8101 atatcatttt ttgatcgatt agatggctaa atgattcaga tggcaattgt ttcccttcta
     8161 tagctaaacc aatgacataa tccctctcac ctattttact caaaatacgt tccccttcta
     8221 tatctaaaat tctttgattt tcagatatac tagctttatc aggtgtcttt tcatcagcta
     8281 gctcaattgt ctcaatttta gaaaagcgat tcagacgttt ctgatactct gcaacacctt
     8341 ctttaaggta tttctctttc aattttccaa ctgtaattat ttttaatttc atagctacat
     8401 tataacataa tccacaatat tttcacaatt tatccacatg tttatcctta catatcccct
     8461 tattttatag gtattctata actataatca aggttatcca caagttgtgg atatttcctt
     8521 tttttagctt ttaaggtata attaagaagc ttgtcataag attagtataa aaatttacgg
     8581 aggtagctat ttgtgaaaaa aaaattagtc tcatcacttc taaagtgttc tctaatcatt
     8641 attgttagct ttgctggtgg agcatttgct agttttgtca tgaatcataa tgacaatatt
     8701 ccaaatggtg gtgtcactaa aactagtaaa gtaaattata ataacataac gcctacaaca
     8761 aaagctgtta aaaaggtaca aaatagtgtt gtttctgtta tcaattataa acaacaagag
     8821 agtcgttctg acctatcaga cttctatagt catttttttg gtaatcaggg gggcaacact
     8881 gataagggct tacaagttta cggtgaaggc tctggagtca tctataaaaa agatggtaaa
     8941 aatgcctatg ttgtcactaa taaccacgtc attgatgggg ctaaacaaat tgaaattcaa
     9001 ctagctgatg gctcaaaagc agttgggaaa cttgttgggt cagataccta ctctgattta
     9061 gccgtcgtca aaattccatc agataaggtt tcaaatattg cagaatttgc tgattcatca
     9121 aaactcaaca ttggtgaaac tgctatagcg atcggaagcc ctcttggaac tgagtatgca
     9181 aattctgtaa ctcaaggtat tgtatctagt ttaaaaagaa ctgtaacaat gactaatgaa
     9241 gaaggacaaa cagtttctac aaatgctatc cagactgatg ctgctatcaa tcctggtaat
     9301 tcaggtggag cacttatcaa tattgaagga caggttattg gaattaattc tagtaaaatt
     9361 tcttctacat caaatcaaac ctcaggacaa tcgtcaggaa atagcgttga aggtatggga
     9421 tttgccattc cttcaaatga tgttgttaag attatcaatc aacttgagag taacggacaa
     9481 gtagagagac ctgctctagg tatttctatg gctggattaa gtaatttacc atccgatgtt
     9541 attagtaaac tgaaaatccc aagtaatgtt actaatggta ttgtagtagc atctatccaa
     9601 tctggcatgc cagctcaagg caaactaaag aaatacgatg tcattactaa agttgacgat
     9661 aaagaagtag tatctccaag tgatttacaa agtttactct atggccacca ggtaggggat
     9721 tccataacag taacctttta tcgtggtgaa aataaacaaa cagtcactat aaaacttact
     9781 aaaactagta aagatttagc taaacaacga gcaaataact aaatcaattc tagttatttt
     9841 aactaagata tagtaaaatt aaaggggttg gtcagataga ccaatccctt tagtaagtct
     9901 atagaatctg aggtctaaaa tggaatattt agaaacgatc aacatcaatc atattgctcc
     9961 aaatccgtat caacctcgcc tagaatttaa tacaaaagaa ctagaagaat tagccaactc
    10021 tattaaaatt aatggcctta tccaacccat tattgtaaga ccgtcagcag tctttggtta
    10081 cgaactggta gccggagaac gacggctaag agccgcaaag ttagctaaat tggaaagtat
    10141 acctgcaatt ataaaatcct ataataatga tgatagtatg caattagcta ttgtcgaaaa
    10201 tttacaacga tctaatttgt cacctattga agaggctaaa gcctactctc aacttctaca
    10261 aaagaaaagt atgacacatg aagagttagc aaaatatatg ggaaaatctc gtccctatat
    10321 atcaaatacg atacgactac taaatttacc gccactaatt acttcagcta ttgaagaagg
    10381 aaaactttcc tctggtcatg cgcgtgcact actcagtcta ccagatgcta gtcaacagaa
    10441 agattggtat cagagaatcc tcactgaaga tattagtgta agacgactag agaagttatt
    10501 aaaacaagaa aagaaaacaa atcataagtc acttcaaaat aaagatgtct ttctaaaaca
    10561 tcaagaaaat gagttagctc aatttctagg tagcaaagtg aaacttacta ttaataaaga
    10621 tggtgctggc aatataaaga ttgctttcgc taaccaagaa gaattaaaca gaattatcaa
    10681 cactctaaaa taagggtgtt gattattttt ttatttaatc aaacttatcc acaaggtatt
    10741 ttgctatttt tctgttgatt ctctaatttc cacagtctgt ggaaaacttt aattaacatt
    10801 gttgatttta ttcttcaaca tctgtggaaa actttatttt tttatggtac aatataacaa
    10861 taattatcca caagacaata aggaagaagc tatgacggaa aacgaacaac tattttggaa
    10921 tagagtacta gagctatctc gttctcaaat agcaccagca gcttatgaat tttttgttct
    10981 agaggctaga ctcctcaaaa ttgaacatca aactgcagtt attactttag ataacattga
    11041 aatgaaaaag ctattctggg aacaaaattt ggggcctgtt atcctaacag ctggttttga
    11101 aattttcaat gctgaaatta cagctaacta tgtctcaaac gatttacatt tacaagaaac
    11161 tagtttttct aactaccagc aatctagcaa tgaagtaaat actttaccaa ttagaaaaat
    11221 cgactctaac cttaaagaga aatatacttt tgctaatttt gttcaaggag atgaaaatag
    11281 atgggctgtt tcagcatcaa ttgctgtagc tgatagtcct ggcacgactt ataatcctct
    11341 atttatctgg gggggacctg gtctaggaaa gacgcatcta ctaaatgcta ttggaaatca
    11401 agtcttaaga gataatccaa acgcgagggt tttatacatc actgctgaga attttattaa
    11461 tgaatttgtc agtcatattc gtttagattc gatggaagaa ttaaaagaaa agtttcgcaa
    11521 cttagactta ctcctgattg atgatattca gtcgcttgct aagaaaacct taggggggac
    11581 ccaagaggag ttcttcaata ctttcaatgc tttacataca aacgataaac aaatcgtatt
    11641 gaccagtgac cgaaatccaa atcaattaaa tgatctagaa gaacgtctag tcacgcgctt
    11701 tagttgggga ctcccagtaa atatcacacc acctgatttt gaaacacgag ttgctatttt
    11761 aaccaataaa attcaagaat atccttatga ttttcctcaa gataccattg aatacttagc
    11821 aggagaattt gattccaacg tacgtgaatt agaaggagcc ttgaaaaata ttagtctagt
    11881 tgctgacttt aagcatgcta aaactattac agtagatata gctgcagaag ctatcagagc
    11941 acgtaaaaat gatggtccta ttgttactgt cattcctata gaagaaattc aaatacaagt
    12001 tggtaaattc tatggcgtaa ctgtaaaaga gataaaagca actaaaagaa cacaagatat
    12061 tgtccttgca agacaggtag ccatgtactt agctcgtgag atgacagata acagtctccc
    12121 aaaaataggt aaagaatttg ggggacgaga tcactcaact gttctccacg cttataataa
    12181 aataaaaaat atggttgctc aagatgacaa cttacgaatt gagatagaaa ctatcaaaaa
    12241 taaaatcaga taggctttgt ggataagtat cagaaaaata cgagactttt ccacaggttg
    12301 tgaacaagtt attttcctta tgaatattaa agttata
//
LOCUS       DATRTN010000052        12275 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.052, whole genome shotgun sequence.
ACCESSION   DATRTN010000052 DATRTN010000000
VERSION     DATRTN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12275
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.052"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..515
                     /locus_tag="VBE22_001356"
     CDS             <1..515
                     /locus_tag="VBE22_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7202815.1"
                     /translation="LTTFALDLIKNKVYKEKDFVTNYKAIVSPLNKEVRGSTFFIKNA
                     QNELEGMLCINLDISAYQNIALDILDLVNLNVNKILPKSPQKISLPQQEEPVEVLSGN
                     IQDIISEIVDPSLLNQNIHLSQEVKVEIVSKLHEKGVFQLKGAVSKVAEVLNISEPSV
                     YRYLKKIETH"
     gene            685..2937
                     /gene="gshAB"
                     /locus_tag="VBE22_001357"
     CDS             685..2937
                     /gene="gshAB"
                     /locus_tag="VBE22_001357"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEO7202816.1"
                     /translation="MIIDRLLQRSHSHLPILQATFGLERESLRIHQPTQRVAQTPHPK
                     TLGSRNYHPYIQTDYSEPQLELITPIAKDSQEAIRFLKAISDVAGRSINHDEYLWPLS
                     MPPKVREEDIQIAQLEDAFEYDYRKYLEKTYGKLIQSISGIHYNLGLGQELLTSLFEL
                     SQADNAIDFQNQLYMKLSQNFLRYRWLLTYLYGASPVAEEDFLDQKLNNPVRSLRNSH
                     LGYVNHKDIRISYTSLKDYVNDLENAVKSGQLIAEKEFYSPVRLRGSKACRNYLEKGI
                     TYLEFRTFDLNPFSPIGITQETVDTVHLFLLALLWIDSSSHIDQDIKEANRLNDLIAL
                     SHPLEKLPNQAPVSDLVDAMQSVIQHFNLSPYYQDLLESVKRQIQSPELTVAGQLLEM
                     IEGLSLETFGQRQGQIYHDYAWEAPYALKGYETMELSTQLLLFDVIQKGVNFEVLDEQ
                     DQFLKLWHNSHIEYVKNGNMTSKDNYIVPLAMANKVVTKKILDEKHFPTPFGDEFTDR
                     KEALNYFSQIQDKPIVVKPKSTNFGLGISIFKTSANLASYEKAIDIAFTEDSAILVEE
                     YIEGTEYRFFVLEGDCIAVLLRVAANVVGDGIHTISQLVKLKNQNPLRGYDHRSPLEV
                     IELGEVEQLMLEQQGYTVNSIPPEGTKIELRRNSNISTGGDSIDVTNTMDPTYKQLAA
                     EMAEAMGAWVCGVDLIIPNATQAYSKDKKNATCIELNFNPLMYMHTYCQEGPGQSITP
                     RILAKLFPEL"
     gene            3061..3879
                     /locus_tag="VBE22_001358"
     CDS             3061..3879
                     /locus_tag="VBE22_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202817.1"
                     /translation="MVKKRYSKNSHNLITLLGIVVLASLISDFWSEVIATLLIIGGGY
                     CAYYVYDKKRLKRFTSNQRIEALKSDIKETDQDIRHLEILKKDNRSKEYIKLAHQILP
                     QLDLIRNEANQLQKAIEPNIYKRITKKANTFSNEINEQLIKLHASPELEPISDQEDEM
                     IRIAPELKPFYHNIQDDHFAILKKIEEADNKAELAAIHQANMKRFTDVLAGYIRIKQS
                     PKNFNNAKERLEQALQAIKKFNLDLDETLRQLNESDMKDFDVSLRMMQDERNSK"
     gene            3876..5132
                     /locus_tag="VBE22_001359"
     CDS             3876..5132
                     /locus_tag="VBE22_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEO7202818.1"
                     /translation="MSDTFNFDIDQIADNAITKTDKTTEIISNQTTSQTGQIAFFEKL
                     TPAQKSAISEKTPALVDTFVGDQNALLDFGQSAVEGVNTTVNHILSEQKKIQIPQVDD
                     LLKNANRELNGFIAKYKDATPAELEKKPNLIQKLFKQSKTSLQEFYFDSQNIEQKMDM
                     MAANVVKQEDTLARNIVSAEMLIEDNTKSIENLVGVIAFIESSQAEAANRASHLQQEI
                     LALDSQTSEYQIKSNQLARMTEVINTLEQQHTEYVSRLYVAWATTPQMRNLVKVSSDM
                     RQKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGVTADAIVNANNAALQMLAETSKEAIP
                     MLEKTAQSPTVSIKSVTALAESLVAQNNGIIAAIDKGRKERAQLESAVIKSAETINDS
                     VKIRDKKIVEALLNEGKSTQEKVDES"
     gene            5272..6147
                     /gene="hslO"
                     /locus_tag="VBE22_001360"
     CDS             5272..6147
                     /gene="hslO"
                     /locus_tag="VBE22_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEO7202819.1"
                     /translation="MDKIIKSISTSGSFRAYVLDCTETVRTAQEKHQTLSSSTVALGR
                     TLIANQILAANQKGNSKVTVKVIGDSSFGHIISVADTKGNVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVVITDYATGQPYTSTTPLITGEIGEDFAYYLTESEQTPSAVGLNV
                     LLDDEDKVKVAGGFMLQVLPGASDEEISRYEKRIQEMPSISSLLESENHIESLLSAIY
                     GEDDYKRLSEDSLAFYCDCSKERFEAALLTLGTKELQAMKDEDKGVEITCQICNQTYY
                     FTEEDLEKIINDSIK"
     gene            6131..7108
                     /gene="dusB"
                     /locus_tag="VBE22_001361"
     CDS             6131..7108
                     /gene="dusB"
                     /locus_tag="VBE22_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEO7202820.1"
                     /translation="MTQLNSSFMIGKVEIPHRTVLAPMAGITNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQSNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPEKIYHIVKEVTSVLDIPLTVKMRTGWSDSSNAIE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLGKVAKAVTSIPFIANGDIRTVHDAK
                     FMIEEIGADAIMVGRGARSNPYIFTQINHFFETGEILPDLPFEKMLDVAEDHLTRLVN
                     LKGETIAVREFRGLAPHYLRGKSGAAKIRGAVSRAETLAEVQELFAGLR"
     gene            7229..7870
                     /locus_tag="VBE22_001362"
     CDS             7229..7870
                     /locus_tag="VBE22_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO7202821.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGQHLGTDVFYEAVDNNPVLDLYYQ
                     DPQKYAFLLQIFFLNKRFQSIKEAYKANNNVLDRSIFEDELFLTLNYKNGNVTKTELD
                     IYKELLANMLEELEGMPKKRPDLLVYIDVSFDKMLERIDKRGRSFEQVDSNPELYDYY
                     KQVHSEYPEWYENYDVSPKIRIDGNKLDFVKNPEDLQHVLDTIDSELQKLDLL"
     gene            7884..8375
                     /locus_tag="VBE22_001363"
     CDS             7884..8375
                     /locus_tag="VBE22_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEO7202822.1"
                     /translation="MIIRPVLPSDAKELLAIYAPYVADTTITFEYDIPSISEFTNRIK
                     NISKSFPYLVAEENGKILGYAYASTYYARAAYDWTCELSIYLDKDARSKKIGSQLYDT
                     LEKELVNQGYVNLLACISLPNDISIAFHKKRGFNQVAHFPKIGFKFEQWHDIVWLQKR
                     LKS"
     gene            complement(8595..11042)
                     /locus_tag="VBE22_001364"
     CDS             complement(8595..11042)
                     /locus_tag="VBE22_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO7202823.1"
                     /translation="MSHYSIKLQEVFRLAQFQAARYESHYLESWHLLLAMVLVHDSVA
                     GLTFAEYESEVAIEEYEAATILALGRAPKEEITNYQFLEQSPALKKILKLAENISIVV
                     GAEDVGTEHVLLAMLVNKDLLATRILELVGFRGQDDGESVRMVDLRKALERHAGFTKD
                     DIKAIYELRNPKKAKSGASFSDMMKPPSTAGDLADFTRDLSQMAVDGEIEPVIGRDKE
                     ISRMVQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGNIPYELRDMRVLELDMMS
                     VVAGTRFRGDFEERMNQIIADIEEDGHIILFIDELHTIMGSGSGIDSTLDAANILKPA
                     LARGTLRTVGATTQEEYQKHIEKDAALSRRFAKVLVEEPNLEDAYEILLGLKPAYEAF
                     HNVTISDEAVMTAVKVAHRYLTSKNLPDSAIDLLDEASATVQMMIKKNAPSLLTEVDQ
                     AILDDDMKSASKALKASYKGKKRKPIAVTEDHIMATLSRLSGIPVEKLTQADSKKYLN
                     LEKELHKRVIGQDDAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKALA
                     EVLFDDESALIRFDMSEYMEKFAASHLNGAPPGYVGYDEGGELTEKVRNKPYSVLLFD
                     EVEKAHPDIFNVLLQVLDDGVLTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFGA
                     KDISHDYTAMQKRIMEELKKAYRPEFINRIDEKVVFHSLSQDNMREVVKIMVKPLILA
                     LKDKGMDLKFQPSALKHLAEDGYDIEMGARPLRRTIQTQVEDHLSELLLANQVKEGQV
                     IKIGVSKGKLKFDIAKS"
     gene            complement(11039..11503)
                     /locus_tag="VBE22_001365"
     CDS             complement(11039..11503)
                     /locus_tag="VBE22_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEO7202824.1"
                     /translation="MAIKNTSDNIEEYIKSLLEQSGIAEIKRSNLADTFQVVPSQINY
                     VIKTRFTESRGYVVESKRGGGGYIRIAKVHFSDQHQLFGNMLSTIGERISEQVFDDLI
                     QLLFDEEIITEREGNLILATSGDDVLGEQASVIRARMLRKLLQRLDRKGFHL"
     gene            11676..12137
                     /locus_tag="VBE22_001366"
     CDS             11676..12137
                     /locus_tag="VBE22_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HEO7202825.1"
                     /translation="MNYIITFLISMIPLVELRGAVPFAIANGIPLWEALAIGVVGNML
                     PVPIIFFFARKVLEWGADKPYTGKFFTWCLKKGHSGGQKLEKVAGEKGLFIALLLFVG
                     IPLPGTGAWTGTLAASLLDWEFKHSVIAVMLGVILAGCIMGTLSIIGFNLF"
BASE COUNT         4077 a         2315 c         2104 g         3779 t
ORIGIN      
        1 ctctaacgac tttcgccctt gatttaatca agaataaagt ttataaggaa aaagattttg
       61 ttactaacta taaggcgatt gtcagccctc tcaataaaga agttcgtgga tctactttct
      121 tcatcaagaa tgctcaaaat gaacttgaag ggatgttatg tatcaacctt gatatttctg
      181 cataccaaaa tattgctttg gatattctag acttagttaa tttaaatgtt aacaaaatac
      241 tacctaagtc ccctcaaaaa atttctctcc ctcaacagga ggaacctgta gaagttctat
      301 caggcaatat tcaggatatt attagtgaga ttgttgatcc tagtctttta aatcagaata
      361 ttcatttaag tcaagaagtt aaggtagaaa ttgtttctaa acttcacgaa aaaggtgtat
      421 tccaattaaa aggtgccgtt tcaaaggtcg ctgaagttct caacatctcc gaaccaagcg
      481 tctatcgtta tcttaaaaaa attgaaactc attaactcta aaactctttt taacttatag
      541 attaataaga ttggactcaa aagttcaatc ttttattttt tatgacaccg gtataagatt
      601 attttagttt tctgtgccgt aaaacttagt ttttattatt tttttgataa actagtatca
      661 tctgttacta ggaggaatgt tgtcgtgatt atcgatcgac tgttacaaag aagccactct
      721 catctaccga ttctacaagc tacatttggc ttagagagag aaagccttcg tattcaccaa
      781 cccactcaaa gggttgctca aacaccccat cccaaaacat tagggagtcg taactatcat
      841 ccttatatcc aaacggatta tagtgaacct caattagaac tcattacacc tattgcaaag
      901 gatagccaag aagccattcg atttttgaaa gctataagtg atgttgctgg acgctctatc
      961 aaccacgatg aatacttatg gccgctttct atgccaccca aagtgaggga agaagacata
     1021 cagattgccc aattagagga tgcttttgaa tatgattatc gcaaatattt ggagaagact
     1081 tatggaaaac taatacaatc catctctgga attcactata atctcggttt aggtcaagag
     1141 ttattaactt ctctctttga attgagtcaa gcagataatg ctattgattt tcagaaccag
     1201 ctttacatga aattgtctca aaatttttta cgttaccgtt ggttactaac ttatctatac
     1261 ggagcgagcc cagtagctga agaagacttt ttagatcaga aactgaataa ccctgtccgg
     1321 tcacttagaa atagtcactt aggttatgtg aatcataaag atattcgtat ttcttatact
     1381 agcttgaagg attatgtcaa cgatctagaa aatgctgtaa aaagtgggca attgattgct
     1441 gagaaagaat tttattcacc tgttcgtctc cgtggtagta aggcctgtcg taattattta
     1501 gaaaaaggaa ttacatattt agagtttcgc acttttgatc tcaacccatt tagcccaatc
     1561 ggtataacgc aggaaactgt tgatactgtt caccttttct tattggcgct tttgtggata
     1621 gattctagta gtcatattga ccaagatatc aaggaagcca atagattaaa tgaccttata
     1681 gcacttagtc atccattaga aaaattacct aatcaagcac cagtttctga cttagtggat
     1741 gctatgcaat ctgttatcca acattttaac ttatcacctt actatcaaga cctattggaa
     1801 tctgtaaaaa ggcaaattca gtcacctgaa ttaacggtag ctggtcaact tttagaaatg
     1861 attgaaggac tttccttaga aacatttgga caaagacaag gacaaattta tcacgattat
     1921 gcttgggaag ctccctacgc cttaaaggga tatgaaacga tggaactttc tacccaattg
     1981 ctactatttg acgttatcca aaaaggagtc aacttcgaag tgctggatga acaagatcaa
     2041 ttcctaaaat tatggcacaa tagtcatatt gagtacgtta aaaatggtaa tatgacctca
     2101 aaagataact atattgtacc gctcgctatg gctaacaaag ttgttacaaa aaaaatccta
     2161 gatgaaaagc atttcccaac tccttttgga gatgaattta ctgaccgtaa agaggcactt
     2221 aactattttt cacaaatcca agataaacca atcgtcgtta agccaaaatc tacaaacttt
     2281 ggtttaggca tttctatttt taaaacatca gctaacttag cttcttatga aaaagcaatt
     2341 gacatcgctt ttaccgaaga tagtgctatt cttgtggaag aatatattga gggtaccgaa
     2401 taccgtttct tcgtccttga gggggactgt attgctgtat tgcttagggt agcagctaat
     2461 gtcgttggag atggtattca tactattagt cagctagtta aacttaaaaa ccaaaaccct
     2521 ctcagaggct atgatcatcg ctctcctcta gaagttattg agctcggaga agtagaacag
     2581 ttaatgttgg aacaacaagg atacactgtt aacagtatcc ctccagaagg aacaaaaata
     2641 gaacttcgcc gtaattctaa tatttccact ggcggtgact ctatagatgt aacaaataca
     2701 atggatccta catataaaca actagcagct gagatggcag aggctatggg ggcttgggtt
     2761 tgtggagttg atttaattat tcctaatgcc actcaagcat actcaaaaga taagaaaaat
     2821 gctacttgca ttgaactaaa ctttaaccca ttgatgtata tgcacaccta ctgtcaagag
     2881 gggcctggtc aatctattac acctcgaatt ttggctaaac tgttcccaga attataacta
     2941 aaaagagttc cttaggctaa gcttaaggaa tttttggtta aaataaagtt atcccttgtc
     3001 atcactagtt catttaagat aaaatattaa caactacaga aagttaggtt aaatattatt
     3061 atggttaaaa aacgctattc caaaaattct cataatctaa taacattact tggtattgtt
     3121 gttcttgctt ccctgatttc agacttttgg agtgaggtga ttgctactct gttaattatt
     3181 ggtggtggtt attgtgccta ttacgtttat gataaaaagc gtcttaaacg ttttacatca
     3241 aaccaacgta ttgaagcttt aaaatccgat attaaagaga ctgaccaaga cattagacac
     3301 ttggaaattt taaaaaaaga caatcgttct aaggaatata tcaaattagc acatcaaata
     3361 ttacctcaat tggacctaat tcgtaatgaa gcaaaccaac tacaaaaagc cattgagcct
     3421 aatatctata aacgtatcac caaaaaagca aatacattta gtaatgagat taacgaacaa
     3481 ctaataaagc tacatgcctc tccagaacta gaacctattt ctgatcagga agatgaaatg
     3541 atacgcattg ctccggaatt aaaaccgttc tatcacaata ttcaagatga ccactttgct
     3601 atcttaaaaa agattgaaga ggcagataac aaagctgaat tagcagcaat tcatcaagcc
     3661 aatatgaaac gttttacaga tgttctggct ggctacatca gaatcaaaca atctcctaaa
     3721 aattttaaca atgctaagga acgcctagaa caagcattac aagcaattaa aaaatttaat
     3781 ttagatttag atgaaacgct gcgccaattg aatgaaagtg atatgaaaga ttttgacgtt
     3841 agcctaagaa tgatgcaaga cgaaaggaac agtaaatgag cgataccttt aattttgata
     3901 ttgaccaaat tgcagacaat gctatcacta aaacagataa aacaacagaa attatttcca
     3961 accagacaac aagccaaact gggcaaattg ccttttttga aaaactaaca ccagcacaaa
     4021 agtctgctat ctctgaaaaa acaccagctt tggtagatac ttttgtcggc gatcaaaatg
     4081 cgctccttga ttttggacaa tccgcagtag aaggcgttaa taccactgtt aatcatatct
     4141 tgtctgagca gaaaaaaatt caaattcctc aagttgatga tttactaaaa aatgctaatc
     4201 gcgaactaaa tggatttatt gccaaatata aagatgctac tccggcagaa ttagagaaaa
     4261 aaccaaactt gattcaaaaa ttattcaaac aaagcaagac ctcgctacag gaattttatt
     4321 ttgactcaca aaacatcgag caaaaaatgg atatgatggc agcgaatgtt gtcaaacaag
     4381 aagatacttt ggcaagaaat atcgtctctg ctgaaatgct cattgaagat aatactaaat
     4441 ctattgaaaa tttggttgga gttattgctt ttattgaatc gagtcaagcc gaggctgcta
     4501 atcgtgcaag ccacttacaa caagaaattc tagcattaga tagccaaacg tccgagtatc
     4561 aaattaaaag taaccaatta gctcgaatga ctgaagttat caataccctc gaacagcaac
     4621 atactgaata tgtcagccgt ctctacgttg catgggcaac aacaccacag atgcgaaact
     4681 tggtcaaagt atcgtcagat atgcgtcaga aacttggcat gttacgtcga aataccattc
     4741 caacaatgaa actctcaatc gctcagttag gcatgatgca acaatctgtc aaatccggtg
     4801 tcactgctga tgctattgtc aacgctaata atgcagcatt gcagatgctg gctgaaacta
     4861 gtaaagaagc gattccgatg ttagagaaga ccgcacaaag ccccactgtt tctattaaat
     4921 ctgtcactgc attagctgaa agcttagtgg ctcaaaataa tggtattatc gctgccatag
     4981 acaaaggacg taaggaacgt gcccaattgg aatctgctgt tattaaatcg gctgaaacaa
     5041 tcaatgattc tgtcaaaatt cgtgataaaa aaatagttga agccttactc aacgaaggta
     5101 aatctaccca agaaaaagtt gatgagtctt aacttactat catatccact aaataaaaac
     5161 gatactgtaa aacacagtat cgtttttata tcattaattt ataccgtttt catctatatt
     5221 ttcctgacta tctccaatgc aaaataagcc ttttatgcta taatatgact tatggataaa
     5281 attataaaat caatttcaac ttccggttct ttccgggctt atgtccttga ttgtacagaa
     5341 actgtaagaa cagctcaaga aaaacatcag acactttcaa gttctactgt agctcttggg
     5401 agaacattaa ttgcaaatca aattttagca gcaaatcaaa aaggaaatag taaagtaact
     5461 gtcaaggtta ttggagatag ctcttttggt catattattt ctgtagctga tactaaagga
     5521 aatgttaaag gttacatcca aaatacgggt gttgatatca agaaaacagc tactggagag
     5581 gtcttagttg gacctttcat gggtaatgga cactttgtcg ttattaccga ttacgcaact
     5641 gggcaacctt acacttcaac aactccacta attacaggag aaattggaga agattttgct
     5701 tattatttaa ccgaaagtga gcaaacacct tctgccgtcg gtttaaatgt cctcttagat
     5761 gacgaggata aggttaaagt tgcaggtgga ttcatgcttc aggtgctccc aggtgcttct
     5821 gacgaagaaa tttctcgcta tgaaaaacgc attcaggaga tgccttctat ttcaagtctg
     5881 ctagagtctg aaaatcatat agaatctctc ctttcagcta tttacggaga agacgactac
     5941 aagcgcttat cggaagactc tttagccttc tactgtgact gttcaaaaga acgttttgaa
     6001 gccgctctat tgactttagg aactaaagaa ttgcaagcca tgaaggatga agataaaggt
     6061 gttgaaatta cttgtcaaat ttgcaaccag acttactatt tcacagaaga agatttggag
     6121 aagataatta atgactcaat taaatagttc atttatgatt gggaaggttg aaattcctca
     6181 tcgtaccgta ctagcaccga tggcaggtat taccaattct gcttttcgta ctatcgctaa
     6241 agaatttggt gctggtctcg ttgttatgga aatgatttct gagaaagggt tactttataa
     6301 caacgaaaag accttacata tgctccatat cgatgaaaat gaacatccta tgtcaattca
     6361 actatttggt ggcgatgcag aaggcttaaa acgtgcagct gactttatcc aaagtaatac
     6421 gaaagcagat atcgtagaca tcaatatggg atgtccagtt aacaaagttg ttaaaaacga
     6481 agctggagct aaatggttac gtgaccctga aaaaatttat catattgtta aagaggtaac
     6541 gtctgtactt gacattccat taacagtaaa aatgcgtaca ggatggtctg actcatctaa
     6601 tgctatagaa aatgcactcg ctgccgaatc tgctggtgta tctgcacttg ctatgcacgg
     6661 tcgaacgcgt gaacaaatgt atactggcac gtgtgaccat gaaacacttg gcaaggtcgc
     6721 taaagctgtt actagtattc cttttattgc taatggtgac attcgtactg ttcacgatgc
     6781 caaatttatg attgaagaga ttggagctga cgctataatg gttggacgtg gtgctcgtag
     6841 caatccttac atctttacac aaattaatca tttctttgaa acaggcgaaa tccttcctga
     6901 cctaccattt gagaagatgt tagatgttgc cgaggatcat ctcactcgac tagttaattt
     6961 aaaaggtgaa actattgccg ttcgtgaatt tcgagggtta gcacctcatt atctgcgtgg
     7021 aaaatcaggc gcagcaaaaa ttagaggagc tgtttcacgt gcagaaacac tagcagaagt
     7081 tcaagaactt tttgcaggtt tacgctaaat caaaaacagt cctttgactg ttttttccta
     7141 tctctgtaca aatttagcat aaatcattgt gtaatgaaaa ctcaaaagta taacaaatta
     7201 ttttagcata acagaattaa ggagaattat gttgattgtt ttagctggaa ccattggagc
     7261 aggtaaaagc tcccttgcag ctgctctagg acaacaccta gggacagatg ttttctatga
     7321 ggcggttgat aataacccag ttttagatct ttactatcaa gatccccaaa aatatgcttt
     7381 tttactacaa atattctttt taaataaacg attccaatct ataaaagaag cctataaggc
     7441 taacaacaat gttttggatc gttctatttt tgaagatgaa ctattcctca ctctaaatta
     7501 taagaatgga aatgtgacta aaacagagct agatatctat aaagaacttc ttgcaaatat
     7561 gttggaagaa ttagaaggta tgcctaagaa gagacctgat ttgctagtct acattgatgt
     7621 ttcatttgat aaaatgttag aacgtattga caaacgagga cgcagctttg agcaagttga
     7681 ttctaaccca gagttatacg actactacaa acaagtccat tctgaatatc cagaatggta
     7741 tgaaaattac gacgtttcac ctaaaattcg gatcgatggt aacaaacttg atttcgtcaa
     7801 aaatccagag gatcttcaac acgtccttga cactattgat tccgaattgc aaaaacttga
     7861 tttactataa ttaggtgata tctatgatta taagacctgt cctaccctca gatgctaaag
     7921 aattacttgc tatctatgct ccctacgttg ctgacactac aataacgttt gaatacgata
     7981 ttcctagtat atcggaattc actaatcgta tcaaaaatat cagcaaatct ttcccatatt
     8041 tagttgctga agaaaatggg aaaatattag gttatgcata tgcttcaact tactacgcta
     8101 gagctgccta tgactggact tgcgagcttt ctatttacct tgataaagat gcacgtagca
     8161 aaaaaattgg tagccaacta tatgatacat tggagaaaga attagttaac caagggtatg
     8221 ttaatctact cgcttgtatc agtttaccaa atgatataag cattgcattc cacaaaaaaa
     8281 gaggatttaa tcaagtcgca catttcccaa aaatcggatt taaatttgaa caatggcatg
     8341 acattgtgtg gttacaaaag cgattgaaat cttaaggtaa tctttagatt tattttaagt
     8401 tagcaaaaga ttaggatatt ataatatatt tatcctaaaa tttttcaacg actttcagtc
     8461 gtacctatat ctacaagtgc aattgttaga tatagatcct tttacttttc ataaatctat
     8521 aagaaaatgt ccaaaatatt ttggacgttt tctttttata ttaaaattcc agtacccatc
     8581 ggtactggaa tgttttatga ttttgcaata tcaaatttca atttgccttt ggatacgcca
     8641 atcttaatta cttgcccctc tttcacttga tttgccaata gtagttctga taaatgatcc
     8701 tctacttgcg tttgaatagt tctacgcagt ggacgagcac ccatttctat atcatagcca
     8761 tcctctgcca agtgtttcaa ggctgaaggc tggaatttca gatccatacc tttatcttta
     8821 agcgctaaaa ttaaaggttt aaccataatt ttcacaactt cacgcatatt atcttgactg
     8881 agactatgga atacaacttt ctcatcaata cgattgataa actctggtcg ataagctttt
     8941 ttaagctcct ccataatacg tttttgcata gcagtataat catggctgat atccttagct
     9001 ccaaaaccaa ctgttttatc gtcacgtaat gcagttgctc ctaaattaga agtcataatg
     9061 attatagtat ttgaaaaatc aacttttcgt cctcgactat ctgttaaaac gccatcatca
     9121 agcacttgta ataatacatt aaaaatatct ggatgagctt tttcaacttc atcaaaaagt
     9181 aaaactgaat atggtttatt gcgtactttt tcagtcaact ctcctccttc atcataacca
     9241 acataacctg gaggtgcccc attcaggtga gaagctgcga atttttccat atattccgac
     9301 atatcaaaac gaattagggc tgattcatca tcaaacaaca cttctgccaa ggcttttgct
     9361 aactctgtct taccaacacc agttggtccc aagaacataa aggaaccaat aggacgctta
     9421 ccagtacgaa tgccagattg attgcgacgg atagcacgac taatggcagt aactgcatca
     9481 tcttgaccga taacacgttt atgcaattct ttttctaaat tgaggtattt tttactatca
     9541 gcttgtgtca atttttcaac aggaatacca gatagacgac tgagagttgc cataatatga
     9601 tcttctgtta cggcaatagg cttacgcttt ttacccttat aacttgcttt tagagcttta
     9661 gaagcagatt tcatatcatc atctaaaata gcttggtcaa cttctgttaa aagcgaagga
     9721 gcatttttct taatcatcat ttgcacagtg gcagaagctt catcaagtaa gtctatagct
     9781 gagtctggta aattcttact tgtcaaataa cgatgagcca cttttacagc agtcataact
     9841 gcttcatctg aaatagtaac attatgaaag gcttcatacg ctggcttcaa tccaagtaga
     9901 atttcatatg cgtcttcaag atttggttct tctactaaaa cttttgcaaa gcgacgagat
     9961 aaagcagcat ctttttcaat atgcttttga tattcctctt gagttgttgc tccaacagtg
    10021 cgtaaagtgc ctcgggccaa agctggtttt aaaatattag cagcatcaag tgtgctatca
    10081 ataccagagc cagaacccat aatagtatgc agttcatcaa tgaaaagaat aatatgaccg
    10141 tcttcttcaa tatctgcaat gatttgattc atacgttcct caaaatcacc acgaaaacga
    10201 gttccagcaa caacgctcat catatctaac tctagaacac gcatatctct aagctcataa
    10261 ggaatatttc cattagcaat acgttgtgct agaccgtacg ccaaagctgt tttaccgaca
    10321 cccgcatctc caactaagac aggattattc tttgttttac gacttaagac ttggaccata
    10381 cgtgaaattt ctttatcacg cccaataaca ggttcaattt ccccatcaac cgccatctgt
    10441 gataaatcac gtgtgaaatc agctaaatca ccagcagtac tcggtggttt catcatatca
    10501 gaaaaagaag cacccgactt tgctttttta ggattacgta attcataaat tgctttgata
    10561 tcatcttttg taaaaccggc atgacgttct agagccttac gtaaatcaac catacgaact
    10621 gattcaccat cgtcttgacc tctaaagcca actaactcta agatacgtgt ggctaataaa
    10681 tctttattaa ccaacatagc aagcaagaca tgttcagtac ccacatcttc agctcctact
    10741 acgatactaa tattttctgc taactttaag atctttttca aagctggtga ttgctctaaa
    10801 aattgataat tggtaatctc ttctttcggt gctctaccta aagctaaaat tgttgctgct
    10861 tcgtactctt caattgcaac ttcagactca tattcagcaa atgtcaaacc agctactgaa
    10921 tcatgaacta aaaccattgc taaaagaaga tgccagcttt ctaaatagtg actttcatag
    10981 cgtgctgctt gaaattgcgc caatctgaat acttcttgta attttatgga ataatgactc
    11041 ataaatggaa tccttttcta tctaatcgtt gtaataattt acggagcata cgagctctaa
    11101 taacagaggc ttgctctcct aagacgtcat cacctgatgt cgccagaatc aaatttcctt
    11161 cacgttctgt aattatctct tcatcgaata ataattgaat caaatcatca aaaacttgtt
    11221 cactgatacg ctcgccaatc gttgataaca tatttccaaa taattggtgt tgatcagaaa
    11281 aatgaacctt agcaatgcgg atgtaacctc caccacctcg tttactctca accacataac
    11341 cacggctttc agtaaaccgt gtcttaatga cataattaat ctgactagga acaacttgaa
    11401 aagtatcagc taaattggaa cgctttattt cagcaattcc tgattgttct aataatgatt
    11461 ttatatattc ttcaatatta tcggaagtat tttttatggc cacaaattca ctccttcctc
    11521 tttgactata actgactatt attatatcaa atttttaaaa gatacggaaa tgaaatctat
    11581 ctaaggacag taaaactaga atgaccgcgg attttagttg tcattactag aaagataaga
    11641 taaaatggaa taataccata gaaaggtttt atttaatgaa ttacattatt acatttttga
    11701 tttcaatgat tcccttagtg gaactacgag gagctgtgcc atttgcaatc gctaatggaa
    11761 tacctttgtg ggaagcgcta gctatcggtg ttgttggaaa catgctacct gtacctatca
    11821 tcttcttctt tgctcgcaaa gttctagaat ggggagcaga taaaccttat actggtaagt
    11881 ttttcacatg gtgccttaaa aaaggacata gcggtggtca aaaacttgaa aaagtagctg
    11941 gagaaaaagg attgtttatt gccttgctat tgtttgtagg cattccctta cccggaacag
    12001 gagcctggac tggcacgctt gcagctagcc tcctagattg ggaatttaaa catagtgtta
    12061 ttgctgttat gctaggtgtt atactagctg gatgcatcat gggaacatta tccattatcg
    12121 gcttcaacct attctagaaa agttaaggtg agatgactgt ctctcctttt taatatttgc
    12181 caatccacca attcgatagc aaaataagac acttgactta agccaagtgt cttattttaa
    12241 aatatattta ctgacaacat tttgctgtca aatta
//
LOCUS       DATRTN010000053        12267 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.053, whole genome shotgun sequence.
ACCESSION   DATRTN010000053 DATRTN010000000
VERSION     DATRTN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12267
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.053"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..585)
                     /locus_tag="VBE22_001367"
     CDS             complement(<1..585)
                     /locus_tag="VBE22_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter (permease)"
                     /protein_id="HEO7202826.1"
                     /translation="MFGKLLKYELKSVGKWYLTLNAAVLLVSIILGLVLKALGGNVST
                     DTNSTSAQIFTIILVLLLAMVISGSLLSTLAIIIKRFYSNIFGRQGYLTLTLPVTTNQ
                     IICSKLLASLLWSIFNIFIVIIGIILVILPLVGIGQFVVAFPEIYKIISSSNAPLFIA
                     YFFLSYVAGTLLIYLSIAVGQLFTNKRVLMGIVSY"
     gene            complement(597..1295)
                     /locus_tag="VBE22_001368"
     CDS             complement(597..1295)
                     /locus_tag="VBE22_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202827.1"
                     /translation="MTQLLQLHHVTKKYHKHTAVNDVTVSIPTGKIIGLLGPNGSGKT
                     TIIKMINGLLQPDKGDIVIDGYRPSVETKKIISYLPDTSYLQENMKIKDVVTLFEDFY
                     NDFDSKVAYQLFEDLNLNPRERLKNLSKGNKEKVQLILVMSRKARLYILDEPIGGVDP
                     AARDYILKTIISNYSNDASVLISTHLISDIEPILDEVIFLKEGEIDLQGNADDLREEH
                     NCSIDALFRERFKK"
     gene            complement(1296..1664)
                     /locus_tag="VBE22_001369"
     CDS             complement(1296..1664)
                     /locus_tag="VBE22_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7202828.1"
                     /translation="MAWEFNEKSPIYSQIAEHIKMQIVSQEIKSGDQLPTVRELAQEA
                     GVNPNTMQRAFTELEREGMVFSQRTSGRFVTEDNLLIGKIRQQVAKAELATFVNNMKK
                     IGYKLDEITVALDHFIKEIN"
     gene            1809..4913
                     /locus_tag="VBE22_001370"
     CDS             1809..4913
                     /locus_tag="VBE22_001370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO7202829.1"
                     /translation="MFAQLDTRTVYSFMDSLVDLKTYVSKSKSLGYQTIGILDHSNLY
                     AAYHFIQEAQKANLRPIVGFSFDIVVENRSIEVYCIAINTVGYKNLLKLSTAQMSEKM
                     SLNLLTEHLEGVQLILPYQDVLDQLNLPFDYVIGVNLTSPQIPYTKPIIAIDTVRYFQ
                     KNDIETLQMLHAIRDNVLLKDAQYASKNQELKPCQEMTLAFKERFPEALANLESLVEN
                     VAYHFDSDFKLPIFNREIPAGEELRTLTQNNLKSKGLWSDAYQERLEKELTIIHKMGF
                     DDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVSYALNITGIDPVKHNLIFERFLNEE
                     RYSMPDIDIDLPDIYRGEFLRYVRNRYGSMHSAQIVTFSTFGAKQAIRDVFKRFGASE
                     YELTNITKKIHFRDNLTSVYNRNLAFRQIIDSKIEYQKAYDIAKRIEGNPRQTSIHAA
                     GVVMSDDLLTDHIPLKNGEDMMITQYDASSVEDNGLLKMDFLGLRNLTFVQKMKEKVD
                     KDYGISIQLETIDLEDKETLKLFAAGQTKGIFQFEQSGAINLLRRIRPECFEDVVATT
                     SLNRPGASDYTENFINRRFGKEKIDLVDPVIAPILQPTYGIMLYQEQVMQIAQTYAGF
                     TLGKSDLLRRAMSKKNSKEMQKMSQSFLEGAVSKGHRQEDARLIFERMAKFAGYGFNR
                     SHAFAYSALAFQLAYFKAHYSDVFYDIMINYSNSDYLIDAIDFGFVIEKPSINTISYR
                     DRIYKKKIYLGLKNIKGVPNDLAYWISKNQPFQSIEDFLMRLPQQFQKSGFISPLIAI
                     GAFDEFDNNRRKITSNLDSLFTFVNELGSLFADTSYHWLEVEDFSNSEKYEMEQDILG
                     VGISPHPLVGISQKASRPFIPISQVQENSEATILVQLKQVKVIRTKSSGQQMAFLTVM
                     DINSKMDITVFPETFNIVRDDLQEGKYYYLHGKIQKRDERLQMVLNGVQEATEERFWI
                     LLKNHDNDKKISEILSKYKGHIPVYLHYETTKETIQSKVHLVRKDSGLALDLSEFVVK
                     TVYQ"
     gene            4994..6016
                     /gene="pfkA"
                     /locus_tag="VBE22_001371"
     CDS             4994..6016
                     /gene="pfkA"
                     /locus_tag="VBE22_001371"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEO7202830.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINQGYYGM
                     VTGDIFPLDANSVGDTINRGGTFLRSARYPEFAELEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDYTIGFDTAVATAVENLDRLRDTSASH
                     NRTFVVEVMGRNAGDIALWSGIAAGADQIIVPEEEFNIDEVVSNVRAGYAAGKHHQII
                     VLAEGVMSGDEFAKTMKAAGDDSDLRVTNLGHLLRGGSPTARDRVLASRMGAYAVQLL
                     KEGRGGLAVGVHNEEMVESPILGLAEEGALFSLTDEGKIVVNNPHKADLRLAALNRDL
                     ANQSSK"
     gene            6065..7567
                     /gene="pyk"
                     /locus_tag="VBE22_001372"
     CDS             6065..7567
                     /gene="pyk"
                     /locus_tag="VBE22_001372"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEO7202831.1"
                     /translation="MNKRVKIVATLGPAVEFRGGKKFGESGYWGESLDVEASAEKIAQ
                     LIKEGANVFRFNFSHGDHAEQGARMATVRKAEEIAGQKVGFLLDTKGPEIRTELFEDG
                     SDFHSYTTGTKLRVATKQGIKSTPEVIALNVAGGLDIFDDVEVGKQILVDDGKLGLTV
                     FAKDKDTREFEVVVENDGLIGKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVNEVRAICEETGNGHVKLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTDKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDKNAQTLLNEYGRLDSSAFPRNNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRSLMINWGVIPVLAD
                     KPASTDDMFEVAERVALEAGLVESGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            7738..8295
                     /gene="lepB"
                     /locus_tag="VBE22_001373"
     CDS             7738..8295
                     /gene="lepB"
                     /locus_tag="VBE22_001373"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO7202832.1"
                     /translation="MVKRDFIRNIILALIAVLILILLRYFVFATFKVHKDATNSYFSN
                     GDVVVVNRNRTPKYKDFIVYKVGKIFYISRVIGEPNQKVRVMDDILYLNDVFKDEPYI
                     EKMKNAYSEKKDGQMPFTSDFSVETLTRNKESRVPKGSYLVLNDNRQNKNDSRKFGLI
                     KEKDIRGVITFKVYPLSEFGFTASE"
     gene            8553..10367
                     /gene="glmS"
                     /locus_tag="VBE22_001374"
     CDS             8553..10367
                     /gene="glmS"
                     /locus_tag="VBE22_001374"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEO7202833.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVVGDNKSQL
                     VKSVGRIAELQAKVGDSVSGTTGIGHTRWATHGKPTEGNAHPHTSGSGRFVLVHNGVI
                     ENYLQIKETYLTKHNLKGETDTEIAIHLVEHFVEEDNLSVLEAFKKALHIIEGSYAFA
                     LIDSQDADTIYVAKNKSPLLIGLGNGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKD
                     SVEVQDYDGNVIERGSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRKLISTYANESG
                     DMNVDSDIIKSVQEADRLYILAAGTSYHAGFAAKTMIEKLTDTPVELGVSSEWGYNMP
                     LLSKKPMFILLSQSGETADSRQVLVKANEMGIPSLTITNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQVATLAFLAKAVGEANGKAEAKDFDLVHELSIVAQSIEATLSEKD
                     VISEKVEQLLISTRNAFYIGRGNDYYVTMEAALKLKEISYIQTEGFAAGELKHGTISL
                     IEDNTPVIALISADSTIAAHTRGNIQEVVSRGANALIIVEEGLEREGDDIIVNKVHPF
                     LSAISMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            10529..10858
                     /locus_tag="VBE22_001375"
     CDS             10529..10858
                     /locus_tag="VBE22_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEO7202834.1"
                     /translation="MSLPNCPKCNSEYVYEDGILLVCPECAYEWNPEEVEEEVGLIVL
                     DSNGTRLSDGDTVTVIKDLKVKGAPKDIKQGTRVKNIRLVDGDHNIDCKIDGFGAMKL
                     KSEFVKK"
     gene            10993..11634
                     /locus_tag="VBE22_001376"
     CDS             10993..11634
                     /locus_tag="VBE22_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7202835.1"
                     /translation="MSYILEILPSLLVGAQVTLQVFFIVICLSIPLGIVVAFLMKVHF
                     KPLQWLLTTYIWIMRGTPLLLQLIFFYYFLPSVGISFDRMPAAIIAFTMNYAAYFAEI
                     FRGGIESIPKGQYEAAKVLKFSKFQTVWYIVLPQVFKIVLPSVFNETITLVKDSSLVY
                     ILGVGDLLLESKTAANRDATLAPMFIAGGIYLLLIGLLTILSKQVEKRFNYYK"
     gene            11645..>12267
                     /locus_tag="VBE22_001377"
     CDS             11645..>12267
                     /locus_tag="VBE22_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7202836.1"
                     /translation="MLELKNISKCYGQKEIFKDFNLTVEEGKILSLVGPSGGGKTTLL
                     RMLAGLEKIDSGTIVHDGKEVSVDHLETLNLLGFVFQDFQLFPHLTVLDNLILSPVKT
                     MGLSKELAKAKALVLLERLGLKDHALVYPFSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQEVEKLILQNRETGMTQIVVTHDLQFAESISDTILKINP"
BASE COUNT         3984 a         1899 c         2415 g         3969 t
ORIGIN      
        1 ataagaaaca attcccatca atacacgttt gtttgtaaat aattgcccta cagcgataga
       61 gagataaatc agtaatgtcc ctgcaacgta cgatagaaag aaataggcga taaaaagtgg
      121 agcgttcgaa gatgaaataa ttttatagat ttctggaaaa gcgacaacga actgaccaat
      181 acctactaaa ggtaatatga ccaatatgat tccaataatg acaataaaaa tattaaaaat
      241 actccaaaga agagaagcta gtagctttga acaaataatc tgattcgtag ttactgggag
      301 cgttaaggtt agatatcctt gacggccgaa gatattgcta taaaaacgtt tgataataat
      361 agcaagggtg gataacagag atcctgaaat aaccatcgct aacaataaaa cgagtattat
      421 agtgaatatt tgggcgctag tactattagt atcagtagaa acattgccgc caagtgcctt
      481 taataccagt cctaaaataa tagatactaa taaaaccgca gcgttgagtg ttagatacca
      541 cttaccaact gattttaatt catattttaa taattttcca aacatatcaa tctcctttat
      601 ttcttgaatc tttcacgaaa gagagcatca atcgaacagt tgtgctcttc tcttaagtca
      661 tcagcattgc cttgtaggtc aatttctcct tctttaagga agataacttc atcaaggata
      721 ggttcaatgt cagaaattaa gtgagtcgaa attaaaacag aagcatcatt agagtagtta
      781 ctaatgattg tttttaaaat gtagtcacgc gctgcagggt ctacaccacc aataggttca
      841 tcaaggatgt agagcctagc tttacgactc ataacaagta ttaattgaac tttttccttg
      901 ttcccttttg ataggttttt tagacgttcc ctaggattta ggttaagatc ctcaaatagt
      961 tgatatgcta cctttgaatc aaaatcgtta tagaaatctt caaataatgt aacgacgtct
     1021 ttgattttca tattttcttg taaatatgaa gtatcaggga gatatgaaat aatttttttg
     1081 gtttcaacag acggtctgta accatcaata acgatatctc ccttatctgg ctgaagtaat
     1141 ccatttatca ttttgatgat tgttgttttt ccgctaccat ttggacctaa taaacctata
     1201 atctttcctg ttggaatact tacagtcaca tcattaacag cagtatgttt gtgatatttc
     1261 tttgtaacat gatgaagttg taataactgt gtcatttaat taatctcctt tataaaatga
     1321 tcgagagcaa ccgtaatttc atctaattta tatcctattt ttttcatgtt gttgacaaaa
     1381 gttgctaact ctgcttttgc aacttgttga cgaattttac caattagtaa gttgtcttct
     1441 gtaacaaagc gtccggaagt acgttgtgag aataccattc cttcacgttc caattcagtg
     1501 aaggcacgtt gcatggtatt tgggttaaca ccagcttctt gagctaactc tctcacggta
     1561 ggcaattggt caccactttt tatttcttga ctaacaattt gcatctttat atgttcagca
     1621 atttgtgaat atataggtga tttttcattg aattcccaag ccatagataa ctcctttcat
     1681 aatcttgtac tttttgtact ataaaaatag tacaaaaaaa tatttgaaat gtcaaggaat
     1741 tatcattgag actttaatcg tttattgttt catagtataa tagtaaaaaa tcgtaaaaag
     1801 gggctactat gtttgcacaa cttgatacaa gaaccgttta ttcttttatg gatagcttgg
     1861 ttgatttaaa gacatatgtc tctaaatcga aatcattagg atatcaaact attggtatct
     1921 tggaccacag taatctttat gcagcatatc attttattca agaggcgcaa aaggcaaacc
     1981 taagaccaat agttggattt agctttgata tagtggttga aaatagatca atagaagttt
     2041 actgtattgc gataaataca gttggttata agaatctctt gaagttgtca acggctcaaa
     2101 tgtctgagaa gatgtctcta aatttattga cagaacatct tgaaggggtg caattgattc
     2161 ttccctatca agatgttctt gatcagctca atttaccctt tgattatgtt attggtgtta
     2221 atttgacctc tccgcagatt ccatatacca aacccattat agccatagat actgttcgat
     2281 attttcaaaa aaatgatatt gagacattac agatgcttca tgctataaga gataatgtcc
     2341 tgttaaaaga cgcacaatat gcgagcaaaa atcaagaatt aaaaccttgt caagaaatga
     2401 ctctagcatt caaggaacgt tttcctgaag ctttagctaa cctagaatct ttggtagaga
     2461 atgttgctta tcactttgat tctgacttta agttacctat ttttaataga gaaattcctg
     2521 ctggagaaga attaagaacc ttaactcaaa ataacttaaa aagtaaaggt ttatggagcg
     2581 atgcttatca agagcgtctc gaaaaagaat taacaatcat tcacaaaatg ggctttgatg
     2641 actatttcct tattgtgtgg gatttattaa gatttggtcg tagtaagggc tactatatgg
     2701 gaatgggacg tggttcagca gcgggaagtt tagtctcata tgctttaaat attaccggta
     2761 tcgatcctgt caaacataat ttaatttttg aacgtttttt aaatgaagag cgttattcga
     2821 tgccagatat tgatattgat cttccggata tttatcgtgg agaattcttg cgctatgtac
     2881 gtaatcgata tggttcaatg cattctgcac agattgtaac cttctcaact tttggtgcga
     2941 aacaggctat tcgagatgtt tttaaacgct ttggtgcatc agagtatgag ttaacaaaca
     3001 taactaaaaa aattcatttc agagataatt tgaccagtgt ttataaccgc aacttagctt
     3061 tcagacaaat aatagatagt aaaattgaat accaaaaagc ctatgatatt gctaagcgta
     3121 ttgaaggaaa tcctagacag acttctattc atgccgctgg ggtagtaatg agtgacgatt
     3181 tgttaacaga tcatattcct ttaaaaaacg gtgaggatat gatgataacg caatatgatg
     3241 ctagttctgt tgaggataat ggtcttctaa aaatggactt tctaggtctt cgaaatttga
     3301 catttgttca gaaaatgaag gagaaagtcg ataaggacta cggtatctct atacaattag
     3361 aaactattga tttagaagat aaagagactc taaaactatt tgcagctggt caaacaaagg
     3421 ggatttttca atttgagcaa agtggagcta ttaatttgtt gcgacgtatt aggccagagt
     3481 gttttgaaga tgtggtagct accaccagct tgaataggcc tggtgcaagt gattatactg
     3541 agaattttat aaaccgtcgt tttggtaaag aaaaaatcga tttagtagat cctgtcatag
     3601 ctccaatttt acaacctacc tatggcataa tgctctatca agaacaggtt atgcagattg
     3661 cccagaccta cgcaggtttt acgctaggca agtctgattt gctcagacgt gctatgtcta
     3721 agaaaaattc taaggagatg caaaagatgt ctcaatcctt cttagagggt gcagtatcta
     3781 agggacatcg tcaagaagat gctcgactta tatttgaacg catggctaaa tttgcaggtt
     3841 atgggtttaa tcgcagccat gcgtttgctt attcggcttt agcttttcaa ttagcctatt
     3901 tcaaagccca ttattccgat gtcttttatg acatcatgat aaattattca aatagtgatt
     3961 atctaatcga tgctattgat tttggttttg ttattgaaaa accttcaatt aatactattt
     4021 catacagaga ccgcatctat aaaaagaaaa tttatttagg tttaaaaaat attaagggtg
     4081 ttcctaacga tttggcttat tggatttcta aaaatcaacc gtttcagagt attgaagatt
     4141 ttctaatgag gctgccacag cagtttcaaa aaagtggttt tatttctccg ctaattgcta
     4201 ttggagcatt tgacgaattt gataataata gacggaaaat cacctctaat cttgattctt
     4261 tatttacatt tgtaaatgaa ctaggtagtt tatttgcaga tacctcatat cattggctag
     4321 aagtagaaga tttctctaat tctgagaaat atgaaatgga acaagatatc ttaggagtag
     4381 gaatcagccc gcacccttta gtggggattt cacaaaaagc tagccgtccg tttataccaa
     4441 ttagtcaagt tcaagaaaat agcgaagcta ctattttagt tcagctaaag caggtcaaag
     4501 ttattaggac aaaaagttct gggcaacaaa tggctttctt gactgttatg gatattaatt
     4561 caaaaatgga tattactgtt tttcctgaaa ctttcaatat tgtaagagat gatttacaag
     4621 aagggaaata ttattacttg catggaaaaa ttcagaagcg agatgagcga ttacagatgg
     4681 ttttaaacgg tgttcaagaa gcaacggagg aacgtttttg gatacttcta aaaaatcatg
     4741 ataacgacaa aaaaatatcg gaaatattgt ccaaatataa agggcatatt cctgtctatt
     4801 tgcattatga aacaactaaa gaaactatcc aaagcaaagt acatttggtt agaaaagata
     4861 gtggcttagc ccttgattta tctgaatttg ttgtgaaaac ggtttatcaa taaaattgca
     4921 tataaagtgc agaaatttac aaattaatat gctataatta aatcgttaaa ttaataaaag
     4981 gagtaccaat taaatgaaac gtattgctgt tttaactagt ggtggtgacg cccctggtat
     5041 gaacgctgct atccgtgcag ttgttcgtaa agcaatttct gaaggtatgg aagtttacgg
     5101 catcaaccaa ggttactatg gtatggtgac aggggatatt ttccctttgg atgctaattc
     5161 tgttggggat actatcaacc gtggaggaac gtttttacgt tcagcacgtt atcctgaatt
     5221 tgctgaactt gaaggtcagc ttaaagggat tgaacagctt aaaaaacacg gcattgaagg
     5281 tgtagtagtt atcggtggtg atggttctta tcatggtgct atgcgtctaa ctgagcacgg
     5341 tttcccagct gttggtttgc cgggtacaat tgataatgat atcgttggca ctgactatac
     5401 tattggtttt gacacagcag ttgcgacagc agttgagaat cttgaccgtc ttcgtgatac
     5461 atcagcaagt cataaccgta cttttgttgt tgaggttatg ggaagaaatg caggagatat
     5521 cgctctttgg tcaggtatcg ctgcaggtgc agatcaaatt attgttcctg aagaagagtt
     5581 caatattgat gaagttgtct caaatgttag agctggctat gcagctggta aacatcacca
     5641 aatcatcgtc cttgcagaag gtgttatgag tggtgatgag tttgcaaaaa caatgaaagc
     5701 agcaggagac gatagcgatc ttcgtgtgac gaatttagga catctgctcc gtggtggtag
     5761 tccgactgct cgtgatcgtg tcttagcatc tcgtatggga gcgtacgctg ttcaattgtt
     5821 gaaagaaggt cgtggcggtt tggccgttgg tgtccacaac gaagaaatgg ttgaaagtcc
     5881 aattttaggt ttagcagaag aaggtgcttt gttcagcttg actgatgaag gaaaaatcgt
     5941 tgttaataat ccgcataaag cggaccttcg cttggcagca cttaatcgtg accttgccaa
     6001 ccaaagtagt aaataagaat ttgtcaattt gacactttaa ataaaaagga gctcataaat
     6061 tattatgaat aaacgcgtaa aaatcgttgc aacacttggt cctgcggtag aattccgtgg
     6121 tggtaagaag tttggtgagt ctggatactg gggtgaaagc cttgacgtag aagcttcagc
     6181 agaaaaaatt gctcaattga ttaaagaagg tgctaacgtt ttccgtttca acttctcaca
     6241 tggagatcat gctgagcaag gagctcgtat ggctactgtt cgtaaagcag aagagattgc
     6301 aggacaaaaa gttggcttcc tccttgatac taaaggacct gaaattcgta cagaactttt
     6361 tgaagatggt tcagatttcc attcatatac aacaggtaca aaattacgtg ttgctactaa
     6421 gcaaggtatc aaatcaactc cagaagtgat tgcattgaat gttgctggtg gacttgacat
     6481 ctttgatgac gttgaagttg gtaagcaaat ccttgttgat gatggtaaac taggtcttac
     6541 tgtgtttgca aaagataaag acactcgtga atttgaagta gttgttgaga atgatggcct
     6601 tattggtaaa caaaaaggtg taaacatccc ttatactaaa attcctttcc cagcacttgc
     6661 agaacgcgat aatgctgata tccgttttgg acttgagcaa ggacttaact ttattgctat
     6721 ctcatttgta cgtactgcta aagatgttaa tgaagttcgt gctatttgtg aagaaactgg
     6781 caatggacat gttaagttgt ttgctaaaat tgaaaatcaa caaggtatcg ataatattga
     6841 tgagattatc gaagcagcag atggtattat gattgctcgt ggtgatatgg gtatcgaagt
     6901 tccatttgaa atggttccag tttaccaaaa aatgatcatt actaaagtta atgcagctgg
     6961 taaagcagtt attacagcaa caaatatgct tgaaacaatg actgataaac cacgtgcgac
     7021 tcgttcagaa gtatctgatg tcttcaatgc tgttattgat ggtactgatg ctacaatgct
     7081 ttcaggtgag tcagctaatg gtaaataccc agttgagtca gttcgtacaa tggctactat
     7141 tgataaaaat gctcaaacat tactcaatga gtatggtcgc ttagactcat ctgcattccc
     7201 acgtaataac aaaactgatg ttattgcatc tgcggttaaa gatgcaacac actcaatgga
     7261 tatcaaactt gttgtgacaa ttactgaaac aggtaataca gctcgtgcca tttctaaatt
     7321 ccgtccagat gcagacattt tggctgttac atttgatgaa aaagtacaac gttcattgat
     7381 gattaactgg ggtgttatcc ctgtccttgc agacaaacca gcatctacag atgatatgtt
     7441 tgaggttgca gaacgtgtag cgcttgaagc aggacttgtt gaatcaggcg ataatatcgt
     7501 tatcgttgca ggtgttcctg taggtacagg tggaactaac acaatgcgtg ttcgtactgt
     7561 taaataattt ttgaattcaa acctattatt taagaatacc ttaaataatg ggttttttat
     7621 atgttttgct ttttattatt cgattattgt gactagaata ggatatgata ttaataatta
     7681 ccctcctttt tttatctatg aaaacatgat ataatggaaa aaagattagg agtagttatg
     7741 gttaaacgcg attttattag aaatattatt ttagcattga ttgctgtatt gattttgatt
     7801 ctcttgagat attttgtttt tgcaaccttt aaagtacata aggatgctac aaattcttat
     7861 tttagcaacg gagatgttgt tgttgttaat cgtaatagga cgcctaagta caaagacttt
     7921 attgtctata aggttggaaa gattttctat attagtcgtg ttattggtga gccaaatcaa
     7981 aaagtgagag taatggatga tattttatat ttgaatgatg ttttcaagga tgagccttac
     8041 attgaaaaaa tgaaaaatgc ttattctgaa aagaaagacg gtcaaatgcc ctttacttcc
     8101 gacttctcag ttgaaacatt aacacgaaat aaagaaagca gagttcctaa gggttcttat
     8161 cttgtgttaa atgataatcg acaaaataaa aatgatagtc gaaaatttgg attaatcaaa
     8221 gagaaagata ttcgtggtgt tattaccttt aaagtatacc ctctaagtga atttggtttt
     8281 acagcatcag agtagtaaaa gaatggtatt atttgatacc attcttttta tgtaataaaa
     8341 tggctaagat taagatagat agaatttttg atagcgttta tctcgttatc acagaaaatc
     8401 aaaaagacta taccaatttt tttattgaca aacaaaaaca ttaattatat actgtttttg
     8461 ttacttttta acggtatata ttaatatata ccaatttaaa aagaaagttt agatagaaag
     8521 tataagagaa agctcttatt ggtaataata atatgtgtgg tatcgtaggt gtagttggaa
     8581 atacaaatgc aacggatatt ttaattcaag gcttagaaaa gctagagtac cgaggatatg
     8641 actcagcagg tatttttgta gtaggtgata acaaaagtca acttgtgaag tcagtcggtc
     8701 gaatcgcgga gcttcaggca aaagtaggag attctgttag tggtacgaca ggtatcggtc
     8761 atacacgttg ggcaacccat ggtaaaccta ctgaaggaaa tgcacatcct cacacttcag
     8821 gatctggtcg ttttgtattg gtacacaatg gtgttattga aaattacctt caaattaaag
     8881 aaacttactt aacaaaacat aacctcaagg gtgaaactga tacagaaata gctattcatt
     8941 tagttgaaca ctttgttgaa gaagataact tatcagtgct tgaagccttt aaaaaagcct
     9001 tacatattat agaaggatca tacgcatttg ctcttattga ttctcaagat gctgatacga
     9061 tttacgtagc taaaaataaa tcacctcttc taataggttt gggaaatggt tataatatgg
     9121 tgtgttcaga tgccatggct atgatacgtg agacaagtga atatatggag atccacgata
     9181 aagaactcgt tattgttaaa aaagatagcg ttgaagttca agattatgat ggtaatgtca
     9241 ttgaacgtgg tagctacaca gcagagcttg acttatcaga tattggtaag gggacttatc
     9301 cattctacat gcttaaagaa attgatgagc aaccaactgt gatgcgtaag ttgatttcaa
     9361 cttatgcgaa cgaatctgga gatatgaatg tggattctga tattataaaa tcagtccaag
     9421 aagcagatcg tctttacata ttggctgcag ggacgtcata tcatgctggt tttgctgcta
     9481 agaccatgat cgaaaaatta actgacacac ctgttgaact tggagtttct tcagaatggg
     9541 gttacaacat gccacttttg agtaagaagc caatgtttat tttacttagt caatcgggag
     9601 aaacagcaga tagtagacaa gttttagtta aagcaaacga aatgggaatc cctagtttaa
     9661 ctataacaaa tgtgcctggc tcaacacttt ctcgtgaagc aacctatacg atgctcattc
     9721 atgcaggacc tgaaatcgct gtcgcttcta caaaagcata tactgctcaa gttgcaacgt
     9781 tagctttctt agcaaaagcg gtaggagaag caaatggtaa ggcagaagca aaagattttg
     9841 atttggttca tgaattgtca attgtagcac aatcaattga agcgacctta tcagaaaaag
     9901 atgttatctc tgagaaagta gagcagttgt taatctcgac acgtaatgcc ttctacattg
     9961 gtcgtggtaa cgactattat gttacaatgg aggctgcact gaagctaaaa gagatttctt
    10021 atattcagac agaaggtttt gctgcaggtg aattaaaaca tggtacaatt tcgttgattg
    10081 aagacaatac tcctgttatt gctttaatat ctgctgattc aactattgca gcgcatactc
    10141 gtggaaatat ccaagaagtt gtttctcgcg gagcaaatgc tttgattatt gtagaagaag
    10201 ggttggaacg tgaaggtgat gatattattg ttaataaagt tcacccattc ttatcagcca
    10261 tttcaatggt tattccgact caattaattg cttactatgc ttcattacaa cgtggtttag
    10321 atgttgataa accacgtaac ttggcaaaag ctgtaactgt tgaatagact cgttatacta
    10381 aaatacgcta atattaacta caaaatatta aaaaatgtaa aaaagcctat ttttgaagga
    10441 aataggcttt ttcctattgt caagaagcct ataatcagtt agaatgttac ttgctgacta
    10501 ttttattaat taatttaagg agagaaaaat gtcattacca aattgcccta agtgtaactc
    10561 tgaatatgtc tatgaagacg gtattttact ggtttgtcca gaatgtgctt acgagtggaa
    10621 tccagaagaa gtcgaagaag aagtaggttt aattgtattg gatagcaatg gaactcgctt
    10681 atcagatgga gatactgtga cagttattaa ggatttaaaa gtaaaaggag cgcctaagga
    10741 tattaagcaa ggcactcgtg ttaaaaatat tcgtttagtt gatggcgatc ataatatcga
    10801 ttgtaaaatt gatggttttg gagctatgaa actaaaatct gagtttgtaa aaaaatagct
    10861 ttcaggcaat tggcaacagt caattgcttt ttgaatatga cttgatttca tgcattttta
    10921 ttaaaaaagc tctatgacat tggtttgaat tttgatataa tgagaacatc tattttttat
    10981 ttagggggga ccatgtctta tattttagaa atcttaccta gcttattagt aggtgcgcaa
    11041 gtaacattgc aagttttctt tattgttatt tgtttatcaa ttccacttgg tattgttgta
    11101 gccttcctaa tgaaagttca ttttaagccg cttcaatggc tactgacaac ttatatttgg
    11161 attatgcgag gcacgcctct gttattacag cttatctttt tttattactt tttaccaagt
    11221 gtagggattt cttttgatcg tatgccagct gctattattg cttttacgat gaattatgca
    11281 gcttattttg cagagatttt tcgaggaggt attgagtcta ttcctaaagg gcaatatgaa
    11341 gctgcaaagg tattaaagtt ttctaagttt caaacggttt ggtacatagt attacctcaa
    11401 gtcttcaaaa tagtcttacc gagtgttttc aatgaaacga ttactttagt gaaagattcc
    11461 tcattggttt acatacttgg tgtgggcgac ttacttctag aaagtaagac agccgcaaat
    11521 agggatgcaa cacttgcacc aatgtttata gcaggtggta tttacctatt attaattggt
    11581 cttttaacta ttttatctaa acaagttgaa aagcgtttta actactataa ataagggagg
    11641 gacgatgtta gaattaaaaa atatttctaa atgctatggg caaaaagaaa tcttcaaaga
    11701 tttcaatctg actgtagaag aaggtaaaat tctctcttta gtaggacctt ctggaggtgg
    11761 aaaaacaaca ctattacgta tgttagctgg actggaaaag attgactcag ggactattgt
    11821 tcacgatggt aaagaagtat ctgttgatca tttggaaaca ctaaatcttt taggattcgt
    11881 cttccaagac tttcaattat tcccacattt aacagtatta gataatttga ttctctctcc
    11941 agttaaaacg atgggattat caaaagaact tgctaaagca aaagccttag tattacttga
    12001 acgcttggga cttaaagacc atgctttagt ctatccattt tcattatctg gggggcaaaa
    12061 gcaacgtgtc gcactagctc gtgcgatgat gattgatcca cagattattg gttatgatga
    12121 gccaactagc gctcttgatc cagagttgcg tcaagaagta gaaaaactaa ttttacaaaa
    12181 tagagaaaca ggtatgacac aaattgtagt aacacatgat cttcaatttg ctgaaagtat
    12241 atctgatacg attctcaaaa ttaatcc
//
LOCUS       DATRTN010000054        12169 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.054, whole genome shotgun sequence.
ACCESSION   DATRTN010000054 DATRTN010000000
VERSION     DATRTN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12169
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.054"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            25..828
                     /locus_tag="VBE22_001378"
     CDS             25..828
                     /locus_tag="VBE22_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO7202837.1"
                     /translation="MKKIIYLGLACVSILTLSGCESIERSLKGDRYVDQKLAENSSKE
                     ATEQLNKETKQALKADKKAFPQLDKAVAKNEAQVLIKTSKGDINIKLFPKYAPLAVEN
                     FLTHAKEGYYNGLSFHRVIKDFMIQSGDPNGDGTGGKSIWNGKDKKKDSGNGFVNEIS
                     PYLYNIRGSLAMANAGADTNGSQFFINQSQQDHSKQLSDKKVPKVIIKAYSEGGNPSL
                     DGGYTVFGQVISGMETVDKIASVEVTKSDQPKEKITITSIKVIKGYKFK"
     gene            complement(880..1713)
                     /locus_tag="VBE22_001379"
     CDS             complement(880..1713)
                     /locus_tag="VBE22_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO7202838.1"
                     /translation="MFTKFFEGLLTYHFLQNAFITAIVIGIVAGAVGCFIILRSMSLM
                     GDAISHAVLPGVAISFILGINFFIGAIVFGLLSSIIITYIKENSVIKGDTAIGITFSS
                     FLALGIILIGLANSTTDLFHILFGNILAVQDSDKYMTIIVGLIVLTLITIFFKELLLT
                     SFDPVLAKSMGMRVSFYHYLLMILLTLVAVTAMQSVGTILIVALLITPAATAYLYVKS
                     LRTMLFLSSALGAVASVLGLYIGYTFNIAAGSSIVLTSTFMFLLAFLFSPKQSLFKKK
                     Q"
     gene            complement(1715..2431)
                     /locus_tag="VBE22_001380"
     CDS             complement(1715..2431)
                     /locus_tag="VBE22_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO7202839.1"
                     /translation="MIISKHLSVSYDNNLVLEDINLRLEGSGIIGILGPNGAGKSTLM
                     KALLGLVDSTGESGIGGDLLPLMGRVAYVEQKTNIDYQFPITVGECVSLGLYKERGLF
                     KRLSKTDWEKVSRVIDQVGLRGFENRPINALSGGQFQRMLMARCLVQEADYIFLDEPF
                     VGIDSISEQIIVNLLKKLSKAGKLILVVHHDLSKVDHYFDQVIILNRHLIACGPIDQA
                     FTRENLSAAYGDAILLGQGD"
     gene            complement(2602..3528)
                     /locus_tag="VBE22_001381"
     CDS             complement(2602..3528)
                     /locus_tag="VBE22_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEO7202840.1"
                     /translation="MKKWLVIVSCFVAFLGLGACANKQDQAKNEKLKVVVTNSILADI
                     TKNIAGNKIDLHSIVPVGKDPHEYEPLPADVEKTTQADLVFYNGINLETGGNAWFTKL
                     IKNAKKKENKDYFAVSDGVDVIYLNGQSGKGKEDPHAWLNLENGIIYSKNIAKQLIAK
                     DPKNKATYEKNRDAYVAKLEKLDKEAKSKFNAIPANKKLIVTSEGCFKYFSKAYGVPS
                     AYIWEINTEEEGTPDQITSLVKKLKQVRPSALFVESSVDKRPMKSVSRESGIPIYAEI
                     FTDSIAKKGQKGDSYYAMMKWNLDKIAEGLAK"
     gene            3695..4342
                     /locus_tag="VBE22_001382"
     CDS             3695..4342
                     /locus_tag="VBE22_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEO7202841.1"
                     /translation="MTPNKEDYLKCIHELGEKNNKISNKKIAEMMQVSAPAVSEMIKK
                     MISEQLIVKDKDLGYYLTKQGLLVVSDLYRKHRLVEVFLVNHLHYTADDIHKEAEVLE
                     HTVSTTFVDQLEKLLDFPQFCPHGGTIPKKGEFLVEINQMTLDQISQLGTYVISRVHD
                     DFQLLKYLEQHRLHINDTIELTQIDPYAKTYHIIYNDENLTIPERIASQIYVSAT"
     gene            complement(4382..5071)
                     /locus_tag="VBE22_001383"
     CDS             complement(4382..5071)
                     /locus_tag="VBE22_001383"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEO7202842.1"
                     /translation="MKIGIIAAMEEELKLLVENLEDKSQETVLSNVYYSGRYGEHELV
                     LVQSGVGKVMSAMSVAILVESFKVDAIINTGSAGAVATGLNVGDVVVADTLVYHDVDL
                     TAFGYDYGQMSMQPLYFHSDKTFVSTFEAVLSKEEMTSKIGLIATGDSFIAGQEKIDV
                     IKGHFPQVLAVEMEGAAIAQAAQATGKPFVVVRAMSDTAAHDANITFDEFIIEAGKRS
                     AQVLMAFLKAL"
     gene            complement(5081..5350)
                     /locus_tag="VBE22_001384"
     CDS             complement(5081..5350)
                     /locus_tag="VBE22_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202843.1"
                     /translation="MGKPLLTDDMIERSNRGEKVSGQTILDQETKIISTEDGMEQLTD
                     ENGKHIYKSRRIENAKRNEFQRKLNLVLFILLILLALLFYAIFKL"
     gene            complement(5350..5904)
                     /locus_tag="VBE22_001385"
     CDS             complement(5350..5904)
                     /locus_tag="VBE22_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO7202844.1"
                     /translation="MDFEEKTINRQTVFDGQIIKVAVDDVELPNGLGQSKRELVFHGG
                     AVATLAVTPEHKIVLVKQYRKAIEGISYEIPAGKLETGESGSKEEAALRELEEETGYT
                     GNLEILYSFYTAIGFCNEKIVLYLATDLQKVENPRPQDDDEVLELLELSYEDCMQMVE
                     KGMIQDAKTIIALQYYGLKMGGHS"
     gene            complement(5925..7304)
                     /gene="glmU"
                     /locus_tag="VBE22_001386"
     CDS             complement(5925..7304)
                     /gene="glmU"
                     /locus_tag="VBE22_001386"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEO7202845.1"
                     /translation="MSNYAIILAAGKGTRMKSDLPKVMHKVSGITMLEHVFRSVQAIE
                     PSKIVTVIGHKAELVRDVLGDKSEFVMQTEQLGTGHAVMMAEEELATSKGHTLVIAGD
                     TPLITGESLKNLIDFHVNHKNVATILTADAANPFGYGRIIRNSDDEVTKIVEQKDAND
                     FEQQVKEINTGTYVFDNQSLFEALKDINTNNAQGEYYLTDVIGIFKEAGKKVGAYKLR
                     DFDESLGVNDRVALATAEKVMRQRIARQHMVNGVTVVNPDSAYIDIDVEIGEESVIEP
                     NVTLKGQTKIGKGTLLTNGSYLVDAQVGNDVTITNSMVEESIISDGVTVGPYAHIRPG
                     TSLAKGVHIGNFVEVKGSQIGENTKAGHLTYIGNAEVGCDVNFGAGTITVNYDGQNKF
                     KTEIGSNVFIGSNSTLIAPLEIGDNALTAAGSTITDNVPIDSIAIGRGRQVNKEGYAN
                     KKPHHPSQK"
     gene            complement(7548..7961)
                     /locus_tag="VBE22_001387"
     CDS             complement(7548..7961)
                     /locus_tag="VBE22_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7202846.1"
                     /translation="MVKALETYIVTNGNGRQAVDFYKDVFQADLVNMMTWEEMDPNCL
                     EDRKDLIINAQLIFDGIRLQISDENPDFVYQAGKNVTAAIIVGSVEEAREIYEKLKKS
                     AQEVQLELQETFWSPAYANLVDQFGVMWQISTELN"
     gene            complement(7974..8351)
                     /locus_tag="VBE22_001388"
     CDS             complement(7974..8351)
                     /locus_tag="VBE22_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7202847.1"
                     /translation="MISSIGQVMLYVSNVEASADFWKNKVGFERVEKQTQGDYVTYIV
                     APKLDSEVSFVLHDKAIIAQMSPELDLATPSILFETTDIDSTYQELTANEVMTNPIVD
                     MGSMRVFNFSDNDNNYFAIREVK"
     gene            complement(8440..9396)
                     /locus_tag="VBE22_001389"
     CDS             complement(8440..9396)
                     /locus_tag="VBE22_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO7202848.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIEGIDLVAILSTVRSLETAKDLA
                     KEYNMSLATSEYKAVLDNEEIDTVYIGLPNHLHFDYAKEALLAGKHVICEKPFTLEAS
                     QLEELVSIANTRQLILLEAITNQYLPNFDLVKEHLSNLGDIKIVECNYSQYSSRYDAF
                     KRGEIAPAFNPEMGGGALRDLNIYNLHLVIGLFGEPITAQYLPNIERGIDTSGVLVLD
                     YGHFKTVCIGAKDCSAEVKSTIQGDKGSIAILGPTNTMPKISLTMNGQESHVYQLNGD
                     RHRMHDEFVIFEGIISNLDFKRAAQALEHSRTVMKVLDMVTK"
     gene            complement(9393..9844)
                     /locus_tag="VBE22_001390"
                     /pseudo
     CDS             complement(9393..9844)
                     /locus_tag="VBE22_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647214.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            complement(9841..10086)
                     /locus_tag="VBE22_001391"
                     /pseudo
     CDS             complement(9841..10086)
                     /locus_tag="VBE22_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3977 family protein"
     gene            complement(10083..10781)
                     /locus_tag="VBE22_001392"
     CDS             complement(10083..10781)
                     /locus_tag="VBE22_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEO7202849.1"
                     /translation="MTKVVLVTGCASGIGYAQAQYFLKQGYQVYGVDKSDKPNLNGNF
                     NFIKLDLSSDLSPLFTMVPTVDILCNTAGILDAYKPLLEVSDEELEHLFDINFFVTVR
                     LTRHYLRRMVEKKSGIIINMCSIASFIAGGGGAAYTSSKHALAGFTRQLALDYAKDCI
                     QIFGIAPGAVQTAMTASDFEPGGLAEWVASETPIGRWTKPSEVAELTGFLASGKARSM
                     QGEIVKIDGGWSLK"
     gene            complement(10774..11010)
                     /locus_tag="VBE22_001393"
     CDS             complement(10774..11010)
                     /locus_tag="VBE22_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEO7202850.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVNGKVLQAT
                     PYFLINVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            complement(11100..11348)
                     /locus_tag="VBE22_001394"
     CDS             complement(11100..11348)
                     /locus_tag="VBE22_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202851.1"
                     /translation="MEKITNDFEKVIAVLNQLSISYELVENGPVLLTEQADNFIVGIE
                     GVRTKIMFLTNKKKTKYYLLIMDDQKLFYPMKTIVAFR"
     gene            complement(11546..12046)
                     /locus_tag="VBE22_001395"
     CDS             complement(11546..12046)
                     /locus_tag="VBE22_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7202852.1"
                     /translation="MACIIRRARLGDEVNLAYIQTESWKAAFGKILPEDIIQKTTEIE
                     PAITMYQQLLHKEVGKGYILEVDSNPHCMAWWDKSREDGMLDYAELICIHSLKEGWGK
                     GYGSQMMNHVLSEIQQAGYNKVILWVFTENTRARKFYDRFGFSFKGKSKTYFEKEEMC
                     YEKILH"
BASE COUNT         3942 a         2483 c         1709 g         4035 t
ORIGIN      
        1 taataactta atcaaggagt atgtatgaaa aaaattattt atcttggttt agcctgtgtc
       61 agcatattaa ctttaagtgg ttgcgagtca attgagcgtt ctctaaaagg agaccgttat
      121 gttgaccaaa aactagctga aaactcttca aaggaagcta ctgaacaact aaacaaagaa
      181 actaagcaag ccttaaaagc tgataaaaaa gcttttcctc aattggacaa ggctgttgcc
      241 aaaaacgagg cacaagtgct tataaaaacc tctaaaggtg atataaatat caagttattt
      301 ccaaaatatg ctcctttagc tgttgaaaat tttctaacac atgcaaaaga aggatactat
      361 aatgggttaa gtttccacag agttatcaaa gattttatga tacaatctgg tgaccctaat
      421 ggagacggaa ccggtggaaa atctatatgg aatggcaaag ataaaaagaa agattctgga
      481 aatggttttg ttaatgaaat atccccttac ctctacaata tcaggggaag tttagcaatg
      541 gcaaatgctg gagctgacac taatggcagc caattcttta tcaatcaaag ccagcaagat
      601 cattccaaac aactgtcaga taaaaaagtt cctaaggtga ttattaaagc ctattcagag
      661 ggaggaaatc caagtttaga tggtggttat accgtctttg gacaagtaat ctctggcatg
      721 gaaactgtgg ataaaattgc ttcggtagaa gttacaaaat cagatcaacc aaaagaaaaa
      781 attactatta caagtatcaa agttattaaa ggctacaaat ttaaataatt ctatcaaaaa
      841 tgagactaga acattttcta gtctcatttt ttaacaacac tattgtttct ttttaaacaa
      901 actttgttta ggagaaaata ggaaagcaag taaaaacata aaagtagatg ttaaaacaat
      961 acttgatcct gcggcaatat taaatgtata accgatatat aaccccagaa cagatgctac
     1021 tgctcctaat gcagatgata aaaacaacat tgttctgaga cttttaacat aaagatatgc
     1081 tgttgcagct ggtgtaatta aaagtgctac gatcaatatt gttcccacac tctgcatagc
     1141 tgttactgca actaatgtta acaaaatcat caataaataa tgataaaaag aaactctcat
     1201 tcccattgat tttgctaaaa caggatcaaa agaagtcaat aataattctt taaagaagat
     1261 agtaatcaga gtcagtacaa ttaatcccac aataatagtc atatatttat cactatcttg
     1321 tactgccaaa atattcccaa aaagaatatg aaagaggtca gttgtactat tagcaagtcc
     1381 aattaaaatg atacctaagg ctaaaaagga cgaaaaagta attccaatag cagtatctcc
     1441 ttttatgaca ctattttctt taatataagt aatgataatc gaagacaaca aaccaaagac
     1501 aatagcacct ataaaaaagt taatccctaa aataaaagat atagcaacac ctggtagaac
     1561 agcgtgagaa atagcatcac ccatcaaaga cattgagcgc aagataataa aacagcctac
     1621 tgcacccgct acaataccaa tcacaattgc agttataaaa gcattttgca aaaaatgata
     1681 agtcaataag ccttcaaaaa atttcgtaaa cattctagtc tccttgtcct aacaaaatag
     1741 catcaccgta agcagcactt aaattctcac gggtaaatgc ctgatctatt ggtccacaag
     1801 caattagatg acggttcaaa ataattactt gatcaaaata atgatcaact ttactcaaat
     1861 cgtgatggac gactaaaatt aattttccgg ccttacttaa tttttttagt aaattaacta
     1921 tgatttgttc actaatggaa tcaatcccta caaacggctc atctaaaaaa atgtaatccg
     1981 cttcttgaac gagacatctt gccataagca tacgttgaaa ttgtccacct gataaggcat
     2041 taattggtct attttcaaac cctctcaaac caacttgatc aatcactcgg gatacttttt
     2101 cccagtcagt ttttgatagt cgcttgaaga ggcctcgttc cttatataat cctaaagaaa
     2161 cgcattctcc aacagttata gggaattggt aatcaatatt agttttttgt tcaacatatg
     2221 ctactctccc cattaaaggt agcaaatctc ccccaatccc tgattctcca gtagaatcaa
     2281 ccaaaccaag aagagctttc ataagcgtag atttccccgc cccattaggc cctaaaatac
     2341 caatgattcc ggagccttct aagcgtaaat taatatcttc tagcactaaa ttattatcat
     2401 aacttacgct taagtgttta ctaataatca tttttcctcc aaaagtagaa tggagctggt
     2461 tataaaacca gactccactt ctctgtattt attaaagcta agtcagtttt tagattagga
     2521 tttcgacatt gttacaactc agccttaagc gagaatacta catcattgat ttcattatga
     2581 tttcaatgtc gattctcact cttattttgc taaaccttct gcaatcttat caagattcca
     2641 tttcatcata gcgtagtagc tgtcaccctt ttgacctttt ttagcaattg agtctgtgaa
     2701 gatttctgcg taaattggga tacctgattc tcttgaaaca gatttcatag gacgcttgtc
     2761 aacacttgat tcaacgaata aagctgaagg tcttacttgt tttaatttct taactaagct
     2821 agtaatttga tctggtgtac cttcttcttc agtgttaatt tcccagatgt atgctgatgg
     2881 aactccataa gcttttgaga aatatttaaa gcaaccttca ctagtaacaa ttaatttttt
     2941 attagctgga atggcattaa atttagattt tgcttcttta tccaattttt ctaatttagc
     3001 tacataagca tctctgtttt tctcataagt agctttgttt tttggatctt tagcaattaa
     3061 ttgcttagca atattttttg agtaaataat accattttca aggttcaacc aagcatgtgg
     3121 gtcttcttta ccttttccgc tttgaccatt tagataaata acatcaactc catcgctaac
     3181 cgcaaagtaa tctttgtttt ctttcttctt agcgttcttt attaattttg taaaccaagc
     3241 attaccgcct gtttcaagat taattccgtt gtaaaaaaca aggtctgctt gagttgtttt
     3301 ttcaacgtct gctggtaatg gctcatattc atgaggatct ttaccaacag gtacaatact
     3361 atgtaagtcg attttgttac cggctatatt ttttgttata tctgccaaaa tagagttggt
     3421 tacaactact tttaattttt cgtttttggc ttgatcctgc ttgttagcac aggcacctaa
     3481 gcctaaaaat gctacaaaac atgatacaat aactaaccac tttttcatca aaatgctcct
     3541 tttaaaatta atataagtta tgtattaaat taagttaact taattttgat attagcataa
     3601 cctattataa agtatttgtc aaatactttt tcagtatttt ttgatataat ggtcttagaa
     3661 aaatagattt taagaagtat aagaaagact gaatatgact ccaaataaag aagattacct
     3721 aaaatgcatt catgaattag gtgaaaaaaa caacaaaatt tccaataaga aaatcgctga
     3781 aatgatgcaa gtctcggctc ctgccgtgtc agaaatgatt aaaaaaatga taagtgaaca
     3841 gctgattgtt aaagataaag atttaggata ttatcttact aaacaaggtt tattagttgt
     3901 ttctgacctt taccgtaaac atcgtctagt agaggtattt ttagttaacc atttgcacta
     3961 tactgctgat gatattcata aagaagcgga agttttagaa catactgtat caacaacttt
     4021 tgttgatcaa ttagagaagt tgttagactt tccacaattt tgtccacacg gtgggactat
     4081 tcctaaaaag ggagagtttt tagtagaaat taaccaaatg acattggacc aaattagtca
     4141 gcttggcacg tatgttatca gtcgtgttca tgatgatttt cagctactga aatacctcga
     4201 acaacaccgt ttgcatatca acgataccat tgaattgaca caaattgacc cttatgcaaa
     4261 aacctatcac atcatctata acgatgaaaa tctaacaatc cctgagcgta tcgcaagtca
     4321 aatatatgta tctgctacat aaataaaaaa gcccgtgtat ctaatgaata cacggacttt
     4381 tttataatgc tttcaaaaat gccattaata cttgggctga gcgtttacca gcttcaatga
     4441 taaattcatc aaatgtgata ttggcatcat gagctgcagt atcactcata gcacgtacca
     4501 ccacaaaggg ctttcctgta gcttgagcag cttgggcaat tgctgcacct tccatctcga
     4561 cagctaaaac ctgtggaaaa tgccccttaa taacatcaat cttttcctgg ccagcaataa
     4621 aactatcccc agttgcaatt aaaccaattt tactagtcat ttcttcttta gataaaactg
     4681 cttcaaaagt agaaacaaaa gtcttatccg aatgaaaata aagtggctgc attgacattt
     4741 gtccatagtc ataaccaaag gcggttaaat ctacatcgtg ataaactaat gtatctgcga
     4801 caacgacgtc tcctacattc aatcctgttg ctactgcacc agctgatcct gtattaataa
     4861 tagcatcaac tttaaagctt tctactaaaa tagcaacact catcgctgac atgactttcc
     4921 caacaccaga ctgtactaaa acaagctcat gttcaccata acgtccactg taatatacat
     4981 ttgataatac tgtttcttga gatttatcct caagattttc cacaaggagt tttaactctt
     5041 cctccatggc tgcaataatt ccaattttca tgatatttcc ttataattta aaaattgcat
     5101 aaaacaagag agccaacaaa ataagcaaaa tgaataaaac taaattcaac ttacgttgaa
     5161 attcatttcg ttttgcattc tcaatccggc gactcttgta gatatgttta ccgttctcgt
     5221 cagttagttg ctccatgcca tcttctgtag aaataatttt agtttcttga tctaagattg
     5281 tttgacctga aactttttca cctcgatttg aacgttcaat catatcatct gttaacaatg
     5341 gttttcccat taagaatgac ctcccatttt taaaccgtaa tattgtaaag caatgatcgt
     5401 tttcgcatct tgaatcatgc ctttttctac catttgcata caatcctcat aacttaactc
     5461 tagaagctca aggacctcat catcatcttg tggcctagga ttttcaactt tttgtaaatc
     5521 agtagctaga tagaggacga ttttctcatt acaaaaacca atagcagtgt agaaactata
     5581 aagaatttca agattccctg tgtagccagt ctcttcttct aattcccgaa gggcagcttc
     5641 ttccttagaa cctgactcac ccgtctcaag tttccctgct ggaatttcgt aagaaatacc
     5701 ttcaatagcc ttacgatact gtttcactaa aacaatttta tgctcaggag ttaccgccaa
     5761 agtagcaaca gctcctccat gaaaaacaag ttcacgtttg gattgtccca atccatttgg
     5821 caactctaca tcatcaacag ctactttgat aatttgtcca tcaaaaacag tttgacgatt
     5881 gattgttttt tcttcaaaat ccataagaac ctcccaaata agtgttactt ttgtgaagga
     5941 tgatgtggtt ttttattggc atatccttct ttattgactt gacgtcctct tccaatagct
     6001 atgctatcaa ttggaacatt atctgtgatg gtagatccag ctgctgttaa agcattatca
     6061 ccaatttcaa gtggtgcaat cagagtagaa ttacttccga taaagacatt acttccgatt
     6121 tctgttttaa atttattttg tccatcataa ttgacagtaa tcgtccctgc gccaaagtta
     6181 acatcacaac ctacctctgc attgccgata taagttaaat gacctgcctt cgtattctct
     6241 cctatttgag aaccctttac ttcaacgaaa ttaccaatat gaacaccttt tgcaagactt
     6301 gttcctggtc taatatgcgc ataagggcct acggttactc catcagaaat aatggattct
     6361 tcaaccatcg aattagtgat ggtaacatca tttccgactt gagcatctac taaataacta
     6421 ccattggtta ataaagtacc tttaccaatc ttcgtctgtc cttttaaagt cacattgggc
     6481 tcaattacag attcttctcc aatctcaaca tcaatatcaa tataagcgga atcaggatta
     6541 accactgtga ccccattaac catatgttga cgtgcaatgc gctgacgcat cactttttca
     6601 gcagttgcaa gtgctacacg atcattaact cctagacttt cgtcaaagtc acgaagttta
     6661 taggcaccta cctttttacc cgcttctttg aaaattccaa taacatcagt tagataatac
     6721 tctccttgag cgttatttgt attaatgtct ttaagtgctt caaatagact ttgattatca
     6781 aaaacataag ttccagtatt aatttcttta acctgctgct caaagtcatt agcatccttt
     6841 tgttctacaa ttttagtaac ttcatcatct gaattacgga taatacgacc atatccaaat
     6901 ggatttgcag catcggctgt taagattgtt gcaacgtttt tatgattgac atgaaaatca
     6961 attaagtttt ttaaactttc tccagtaatc aggggtgtat cacctgcaat aactagtgta
     7021 tgtccttttg atgtcgctaa ttcttcttcc gccatcatta cagcatggcc agttcctagc
     7081 tgttctgttt gcattacaaa ctctgatttg tcacctaaaa catcacgtac aagctccgcc
     7141 ttatgtccaa taacagtaac aattttactt ggctcaatag cttgcacact acggaaaaca
     7201 tgctcaagca tggtaatacc agatacttta tgcataactt ttggtaaatc agatttcatg
     7261 cgagttccct tacccgcagc taaaataatg gcataatttg acatattaat tcctctatct
     7321 ctgaatataa ttcaacctat tatatcataa tgccttcaac ttatcactaa taccccttta
     7381 aatccctatt acaatagact ttagctaaaa tagcttactt caattaggaa taaaatagct
     7441 accttttggt agctattcaa tacatgaaat ccgttaattt tttcttctct gtttgcaatc
     7501 agtattttta taaatctatc attgcaatgc tcagatggta aaaacgatta gtttaactct
     7561 gttgaaattt gccacatcac accaaattgg tctactaagt ttgcgtaagc aggactccag
     7621 aaagtttctt gtaactctaa ctgaacttct tgtgcactct tctttaattt ttcataaatt
     7681 tcacgcgcct cctctacgct acccacaata atagcggctg taacattttt accagcttga
     7741 tacacgaaat ctggattttc atccgagatt tgaagacgaa tgccatcaaa aataagctga
     7801 gcattaatga ttaaatcctt gcggtcttca agacagttgg ggtccatctc ttcccatgtc
     7861 atcatattca ctaaatccgc ttgaaagaca tctttataaa aatcaaccgc ttgtcgccca
     7921 tttccatttg ttacgatgta tgtttctagt gctttaacca taagttctcc tttttatttt
     7981 acttcacgga ttgcaaagta attattgtca ttatcggaaa aattaaaaac tctcatactc
     8041 cccatatcaa caataggatt tgtcataacc tcatttgctg ttaattcttg atatgtactg
     8101 tcaatatcag tagtttcaaa taaaatagag ggagttgcta aatctagctc aggactcatt
     8161 tgagctataa ttgctttgtc atgtaagacg aagctaactt cactatccaa cttcggtgca
     8221 actatatagg ttacataatc tccttgtgtc tgtttttcaa cacgctcaaa acccaccttg
     8281 tttttccaaa aatctgctga tgcttcaaca ttgctaacgt agagcattac ctgtccaatt
     8341 gatgaaatca ttttattatc tcactttcgg ttttattact aattagtata acatacagta
     8401 tttaaaataa gtaaaaaatg actcaaaaga gtcatttact tatttcgtta ccatatctag
     8461 aactttcatc accgttctac tatgttctaa agcttgagca gcacgcttaa aatctaaatt
     8521 gctaataata ccttcaaaaa taacaaattc atcgtgcatg cggtgcctat caccatttaa
     8581 ttgataaaca tgactttctt gtccattcat agttaatgaa attttaggca tagtattagt
     8641 tggccctaaa atcgctatag accccttatc accttgaata gtagatttta cttctgcact
     8701 acaatctttt gctccaatac agactgtttt aaagtgccca tagtctagga ctagaactcc
     8761 tgaagtatca ataccacgct ctatatttgg taaatactgt gctgttatag gttctccaaa
     8821 aagtccaatt actaaatgta aattataaat attaaggtct ctcaacgcac ctccacccat
     8881 ttcaggattg aaagctggtg ctatttcccc acgtttaaag gcatcataac gcgatgaata
     8941 ttgggaataa ttacattcta caattttaat atctccaagg ttgcttaaat gttcttttac
     9001 aaggtcaaaa ttaggtaaat attgatttgt aattgcttcg agtaatatta gttgcctagt
     9061 attggcaata gatacaagtt cttctaattg acttgcttct aatgtaaatg gcttctcaca
     9121 aatgacatgt tttcctgcca agagtgcctc cttagcatag tcaaaatgca agtgattcgg
     9181 cagtccaata tagactgtgt caatctcctc attgtccaag acagccttat attcactcgt
     9241 agctagagac atgttatatt ccttagctag atctttagct gtctccaaac ttctaactgt
     9301 tgataagata gcaactagat caataccttc aattttctgc aagacaggca aaacttcctt
     9361 aacaatcata cctgtcccca aaaccgctaa tttcattcgt taatctcctt tgggtaaacc
     9421 acttggaatt cctctaaaac tatcctagag tcctcactaa acattaaacc aaactcctca
     9481 aaataaggtt taaagaaatc aatgtgagct tttctccacc attctaaagt tttatctcct
     9541 tctccttctt taaaagcatg gtctgcagag acatctttaa aagggacaat atcaacttta
     9601 gttatttcaa taatacagat agcttcatca ttgctatcca aaataatatc atatgaacca
     9661 acttgtgtaa cccttcctca tctaattcat ataaatcata ggcagaggcc gttgcagttt
     9721 ttgtcccatc tacaactaac tttgccagaa gatctggttc tgctccaaat tgccatgcgt
     9781 ctatctcatc accgattgaa ggattcaaag atttaaactt attccataat tcttgtgcct
     9841 tcatattacc tccgaagcaa ttcccacaac aaacttaact gcgttactca tttttctttg
     9901 tcttttaaat ccttctttaa catctaaaat ataaacagtt tgaccaatcc aaacacgtaa
     9961 ataaacagat tttattaaaa tatcacctga tattccttta acttcccgct cagtaccatc
    10021 agatgcttca aattccgtac gtaataacca agtattacca caaccaaatt caatctattt
    10081 tctcatttca aactccagcc accatcaatt ttgacaattt caccttgcat tgatctcgct
    10141 tttcctgaag caagaaatcc agttaattca gcaacttcac ttggtttagt ccaacgacca
    10201 attggcgtct cactagctac ccattctgct aatccaccag gttcaaaatc actagcagtc
    10261 atagcagttt gtacagcacc tggcgctata ccaaaaattt gaatacaatc ttttgcgtaa
    10321 tccaaagcca attgacgtgt gaatcccgca agggcatgtt tagaactagt atatgcagct
    10381 ccaccgccac ccgctataaa acttgctatt gagcacatat tgataataat acctgacttc
    10441 ttttccacca tacgtctaag ataatgacgt gttagcctaa cagtcacgaa aaaattaata
    10501 tcaaataggt gttccagttc ttcatcactt acttctagaa gtggcttgta ggcatctaga
    10561 atacctgcag tattacacaa aatatcaaca gtcggtacca ttgtgaatag tggggataaa
    10621 tcagaggata aatccaactt tataaagtta aaattaccat ttaagttcgg tttatccgac
    10681 ttatccacac catatacttg atacccttgt ttcaaaaaat attgagcctg tgcataacca
    10741 attccactag cacagcccgt cactaaaaca actttagtca ttgacctcga tccaatcctc
    10801 tgctaaaaca tcacatggtg ttggtgccca catggaaaaa ccttcacctt caccagtaac
    10861 attaataaga aaataaggtg tagcctgtag tactttccca ttaacttcaa gggtatcaaa
    10921 taactgaaca taattttcag cgccccccca acctgtacgt acatattttt tctttgcttt
    10981 taaacctggt aaaatttctt caaatgtcat attgttctcc tttattgtct ttaaccattg
    11041 atatatcaag gtttattgct tatttaaaat cataatttaa agtttcttcg ccttttagtt
    11101 catctaaagg ctactattgt tttcatagga taaaatagtt tctggtcatc cattattagt
    11161 aaataatatt tcgtcttttt cttgttggtc aagaacatta tttttgttct tactccttca
    11221 attcccacaa taaagttatc tgcttgctcc gtcaacaaaa caggtccatt ttccactaat
    11281 tcataagaaa tacttagttg atttagcaca gctataactt tttcaaaatc attcgtaatt
    11341 ttctccattc ttccatagag ttagcataac tatttgtaga ctagagaacc taaaattgat
    11401 acactgtttt tcactcaaaa aattttacct tcataatctt cattatacct ataacaattt
    11461 tcttttacaa atttgttccc cttaaaggaa attgtttttc aaaataggat gtttatacta
    11521 ttgtattatc ggatgcttta tctcgctagt gcaaaatctt ctcataacac atttcttcct
    11581 tttcaaaata agtttttgat ttacccttaa aagaaaaacc aaacctatcg taaaatttcc
    11641 ttgcgcgtgt attttctgta aatacccaaa gtatcacttt attataacct gcttgttgta
    11701 tctctgatag tacatgattc atcatttgag acccataacc ttttccccaa ccttctttaa
    11761 gactatgtat acaaatgagt tctgcataat caagcatacc atcttctcta cttttatccc
    11821 accaagccat acaatgagga ttgctatcta cttccaatat atacccctta ccaacctcct
    11881 tgtgtagtag ctgttgatac attgttatgg caggttctat ttccgtagtt ttttgaatga
    11941 tatcctcagg tagtatctta ccaaaagcag ctttccagct ctccgtttga atataagcta
    12001 aattgacttc atctcctaat cttgctctcc tgattatgca agccataact aaaatccttt
    12061 caaaaattat tattaactgc ctgataacta ctccaattat accattttcg tgatattaat
    12121 ttttattttg aacagttctg gttctcattt atcattatat ttctaaaat
//
LOCUS       DATRTN010000055        11358 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.055, whole genome shotgun sequence.
ACCESSION   DATRTN010000055 DATRTN010000000
VERSION     DATRTN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11358
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.055"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(63..1283)
                     /locus_tag="VBE22_001396"
     CDS             complement(63..1283)
                     /locus_tag="VBE22_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEO7202853.1"
                     /translation="MKNLNRYVVAVSGVVLHLMLGSTYAWSVFRNPIISETGWDISSV
                     SFAFSLAIFCLGMSAAFMGHLVERFGPRIMGMISAILYGAGNVLTGLAIETQQLWLLY
                     VAYGILGGIGLGSGYITPVSTIIKWFPDRRGLATGFAIMGFGFASLVTSPLAQSLMIR
                     IGVGKTFYILGLVYFFVMMIASQFIKQPPQEKITILTHDGKKNAMNSQIITGLKANVA
                     IKSKTFYIIWLTLFINISCGLGLISAASPMAQDLAGYSAESAALLVGVLGIFNGFGRL
                     LWASLSDYIGRPLTFIILFIVNFIMTSSLFLSFNAIVFAIAMSILMTCYGAGFSLLPA
                     YLSDIFGTKELATLHGYSLTAWAIAGLFGPLLLSKTYSWGNSYQLTLMVFGFLFLFGL
                     LLSLYLRKLTTKVV"
     gene            complement(1470..2837)
                     /locus_tag="VBE22_001397"
     CDS             complement(1470..2837)
                     /locus_tag="VBE22_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEO7202854.1"
                     /translation="MKLSLHEVAKVVGAKNQVSEFEDVPLGNIEFDSRNISEGDLFLP
                     LKGARDGHEFIEMAFDNGAIATISEKEIEGHPYLLVSDALKAFQVLAQYYIEKMNVDV
                     IAVTGSNGKTTTKDMIAAILSTTYKTYKTQGNYNNEIGLPYTVLHMPEDTEKIVLEMG
                     QDHLGDIHVLSEIAKPRIAVVTLIGEAHLEFFGSREKIAEGKMQITDGMSSDGILIAP
                     GDPIIDPYLPANQMTIRFGHDQELQVTELKEEKHSLTFKTNALEHQLRIPVPGKYNAT
                     NAMVAAYVGKLLAVAEEDIVDALENLQLTRNRTEWKKSANGADILSDVYNANPTAMRL
                     ILETFSAIPNNDGGKKIALLADMKELGEQSVDLHNQMIMSIRPDSIDTLICYGQDIEE
                     LAQLASQMFPIGKVYFFKKNQEVDQFDQLLAKVKDTLKEKDQILLKGSNSMNLSKIVD
                     ILEVE"
     gene            complement(2984..4030)
                     /locus_tag="VBE22_001398"
     CDS             complement(2984..4030)
                     /locus_tag="VBE22_001398"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEO7202855.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIT
                     QVGQFIKTQEFDEMPSSDEKLMTNQTVDLDKMVRPSDIYDDNAIVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKQVLATVGVPQVAYQTYFEGDDLEHAIKL
                     SLETLSFPIFVKPANMGSSVGISKATDESSLRSAIDLALKYDSRILIEQGVTAREIEV
                     GILGNNDVKTTFPGEVVKDVDFYDYDAKYIDNKITMDIPAKVDEATMEAMRQYASKAF
                     KAIGACGLSRCDFFLTKDGQIFLNELNTMPGFTQWSMYPLLWENMGLTYSDLIEKLVM
                     LAKEMFEKRESHLI"
     gene            complement(4171..4767)
                     /gene="recR"
                     /locus_tag="VBE22_001399"
     CDS             complement(4171..4767)
                     /gene="recR"
                     /locus_tag="VBE22_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEO7202856.1"
                     /translation="MLYPTPIAKLIDSFSKLPGIGTKTATRLAFYTIGMSDEDVNEFA
                     KNLLAAKRELTYCSVCGNLTDDDPCLICTDKTRDQSVILVVEDSKDVSAMEKIQEYNG
                     LYHVLHGLISPMNGISPDDINLKSLITRLMDGQVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(4782..6827)
                     /locus_tag="VBE22_001400"
     CDS             complement(4782..6827)
                     /locus_tag="VBE22_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEO7202857.1"
                     /translation="MLNRKKRYRLTVKKQNASIPRRLNLLFFIIVLLFTVLILRLEQM
                     QIGQQSFYMKKLTALTSYTVKESKARGQIFDAKGVALVENDERPTVAFSRGNNISSQS
                     IKELANKLSHYITLTEVASSDRAKRDYYLADKANYKKVVESLPDSKRYDKFGNHLAES
                     TVYANAVAAVPVSAINYSEDELKVVALFNQMNATPTFGSVKLSTGELSDDQIKKLDAD
                     KKELLGISVTSNWHRRKKGTSLSDILGTISTEKAGLPREEVKKYLKKGYSLNDRVGTS
                     YLEKQYEDDLQGIRQIRKVVVNKKGKVVSDNITQEGKSGRNLKLTIDLNYQNKVESIL
                     KQYYGSELSSGRASFSEGMYAVAIEPSTGKVLAMAGLKNDHGNLVDDSLGTIAKNFTP
                     GSVVKGATLSSGWENKVLRGNEVLYDQEIANIRSWFTRGLTPISAAQALEYSSNTYMV
                     QVALRLMGQDYNTGDALTDRGYQEAMAKLRKTYGEYGLGVSTGLDLPESEGYVPGKYS
                     LGTTLMESFGQYDAYTPMQLGQYISTIANNGNRLAPHVVSDIYEGNDSNKFAQLVRSI
                     TPKTLNKIAISDQELAIIQEGFYNVVNSGSGYATGTSMRGNVTTISGKTGTAETFAKN
                     VNGQTVSTYNLNAIAYDTNRKIAVAVMYPHVTTDTTKSHQLVARDMIDQYISQFTGQ"
     gene            complement(6956..7648)
                     /locus_tag="VBE22_001401"
     CDS             complement(6956..7648)
                     /locus_tag="VBE22_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEO7202858.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIQ
                     AAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPDMAKDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFEFDE
                     KLNLVSEYYLGK"
     gene            complement(7825..8583)
                     /locus_tag="VBE22_001402"
     CDS             complement(7825..8583)
                     /locus_tag="VBE22_001402"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEO7202859.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFIEAVASKLPSSELVEAGIAAP
                     ALTLSTVLEAAKGSELKIAAQNSYFENSGAFTGENSPKVLAEMGTDYVVIGHSERRDY
                     FHETDQDINKKAKAIFANGLTPIICCGESLETYEAGKAVEFVGAQVSAALAGLSEEQV
                     SSLVIAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQAVADKVRVQYGGSVKPE
                     NVAEYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(8764..9960)
                     /gene="tuf"
                     /locus_tag="VBE22_001403"
     CDS             complement(8764..9960)
                     /gene="tuf"
                     /locus_tag="VBE22_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO7202860.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPTSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDEKYEDIIMELMSTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEVEIVGIKEDIQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVLAKPGSINPHTKFKGEVYILSKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIEVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            complement(10304..11335)
                     /locus_tag="VBE22_001404"
     CDS             complement(10304..11335)
                     /locus_tag="VBE22_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEO7202861.1"
                     /translation="MAVLVEVFLLLIARFFTQEVNGAHGWIVIGPISFQPAEYLKVII
                     VWYLAFTFARRQKKIEIYDYQALTKGRWLPRSLSDLKDWRFYSLFMIGLVIAQPDLGN
                     GSIIVLTVIIMYCISGIGYRWFSALLGLIVVGSTLFIGTIAVVGVETMAKVPVFGYVA
                     KRFNAFFDPFKDLTDSGHQLANSYYAMSNGGWFGRGLGNSIEKLGYLPEATTDFVFSI
                     VIEELGVIGAGFILALVFFLILRIMHVGIKAKDPFNSMIALGIGAMLLMQVFVNIGGI
                     SGLIPSTGVTFPFLSQGGNSLLVLSVAIGFVLNIDANEKKELIMKEAEEQYKPQEKNE
                     KIINLDAFK"
BASE COUNT         3596 a         2282 c         1792 g         3688 t
ORIGIN      
        1 aaaactattt ttcacagaaa aaactctttg gcaaaaccaa agaattttaa attagatata
       61 tactacacaa ctttagttgt taattttctt aaatatagag ataacaataa tccgaataag
      121 aataaaaaac caaaaaccat taatgtcaat tgataggaat ttccccatga atatgtcttt
      181 gataacaata ggggcccaaa cagacctgct attgcccatg ctgttaaact ataaccatgt
      241 aaagtagcta attcctttgt tccaaaaata tcacttagat aagcaggtaa taaggaaaaa
      301 cctgcaccat aacaagtcat taaaatagac atcgctattg caaatacaat agcattgaat
      361 gacaaaaata aactagaagt cataataaag ttcacaataa ataatattat aaaggtcaac
      421 gggcgtccaa tgtagtcaga gagacttgcc cataacagac gtccaaaacc gttaaatatc
      481 cctagtaccc ctactaataa ggctgcagat tctgcggaat agcctgctaa atcttgtgcc
      541 attggtgaag ctgctgatat taaacctaag ccacacgaaa tattaataaa caaggtcaac
      601 caaatgatgt aaaaggtttt tgattttata gcaacgtttg cttttaatcc agtgataatt
      661 tgtgaattca tagcattctt tttaccgtcg tgagtcaaaa tagttatttt ttcctgaggt
      721 ggttgtttaa taaattgtga ggcaatcatc atgacaaaaa aatatactaa tcccaaaata
      781 taaaatgttt tacctacacc aatcctaatc attaaggatt gtgcaagtgg acttgttact
      841 aaagaagcaa agccaaatcc cataatagcg aatcctgttg ctagtcccct cctatcagga
      901 aaccatttaa taatagtcga tactggagta atataacctg aaccaagtcc gattcctcct
      961 aaaataccgt atgcaacata cagtaaccat aactgctgag tttcaatggc taagcctgtt
     1021 aacacattcc ctgctccata taaaatagca gaaatcattc ccattatcct aggaccaaaa
     1081 cgctctacta agtgtcccat aaaagctgca gacattccta gacaaaaaat agccaaacta
     1141 aaagcgaatg aaactgatga aatatcccaa ccagtctctg agataattgg gttacgaaac
     1201 acactccaag cataagttga tcctagcatt aaatgtaaaa cgactccaga aaccgcaact
     1261 acataacgat ttaaattttt caaacaatcc tcctaaaaac gaacttaagt tttattttta
     1321 aaaatatagt tcgtaataaa cagattatca aaaaactgaa tatatcacca gttttctgct
     1381 tagcatatct attttcctca ataaaactga atattattcg tgattaaaaa acaaaaaagt
     1441 tagggtaact tgttacccta acccctctat tattctactt caagaatatc aactatttta
     1501 gaaagattca ttgagttaga accttttaat aatatttgat ctttctcctt aagagtatct
     1561 tttacttttg caagcaattg atcaaactga tccacttctt gattcttttt aaagaaatac
     1621 actttaccaa ttgggaacat ctggcttgct aattgagcca actcctcaat atcctggcca
     1681 taacaaatca aagtatcaat agagtcaggt ctgatactca taatcatttg attatgtaaa
     1741 tccacagatt gttctcccag ttctttcata tcagctaaaa gagcaatctt ctttccacca
     1801 tcattattcg gaatagctga aaaggtttct agtatcaatc gcatagccgt tgggttagca
     1861 ttgtaaacat ctgataaaat atctgctcca ttggctgatt tcttccattc ggtgcgatta
     1921 cgagttagtt gaagattttc tagagcatcc actatatctt cttctgctac tgccaataat
     1981 tttccaacat aggcagcgac catcgcatta gttgcattgt acttaccagg tactggtata
     2041 cgtaattggt gctccaaagc attagtttta aaagttaagg aatgtttttc ttctttgagt
     2101 tctgtcactt gcaattcttg gtcatgacca aaacgaatag tcatctggtt tgctggaaga
     2161 tatggatcaa taattggatc tcccggtgca attaatatgc catctgaact cataccatct
     2221 gtgatttgca ttttcccttc tgcaatcttt tctcgactac caaaaaattc taagtgtgct
     2281 tcaccaatca aagtaacaac agcaatacgt ggttttgcaa tttcagataa cacatggata
     2341 tctccaagat gatcctgtcc catctccaaa acaatttttt cagtatcttc tggcatgtgt
     2401 agcacagtat agggtaagcc aatctcgtta ttgtagtttc cttgtgtttt ataagtctta
     2461 tatgtggttg ataagattgc agctatcata tctttagttg ttgtcttacc atttgagcca
     2521 gttactgcaa taacatcaac gttcatcttt tcaatataat actgtgccaa gacttgaaat
     2581 gctttaagtg catcagacac taaaagataa ggatgaccct ctatttcctt ctctgaaatg
     2641 gttgctattg ctccattatc aaaagccatc tctataaact catgtccatc tcttgctcct
     2701 tttaagggaa gaaataagtc accttcactg atattacgac tatcaaattc aatatttccc
     2761 aggggaacgt cttcaaactc tgatacttga tttttagcgc caactacttt agcaacttca
     2821 tgcaaactaa gcttcatatc atttcctttt ctattattta gcaacaatgt tgtagatagt
     2881 atgctatccc ttataaacga atatactcct aaattataac acactttcat aacatatcat
     2941 agatttgaaa aagctcaact aagaaagact tagttgaact tttttaaatt aaatgactct
     3001 cacgtttttc aaacatttct ttagctaaca taactaactt ttcaatcaaa tcactataag
     3061 ttagccccat attttcccaa agcagaggat acattgacca ctgagtaaaa ccgggcattg
     3121 tattcagttc gtttaagaag atttgtccat ctttcgtcaa aaagaaatca cagcgtgata
     3181 aaccacaagc cccgattgct ttaaaagctt tacttgcata ttgacgcatt gcttccatag
     3241 ttgcttcatc aactttagct ggaatatcca tagtaatttt attatcaata tatttggcgt
     3301 catagtcata gaaatcgacg tctttaacga cttcgccagg aaaagttgtc ttaacatcat
     3361 tattgcctaa aatacctact tcaatttcac gagctgtcac gccttgttca atcaaaatac
     3421 ggctatcata cttgagagct aagtcaattg cagagcgaag tgaggattca tctgtcgctt
     3481 ttgaaatacc tactgatgac cccatattag ccggttttac aaaaattggg aaacttaaag
     3541 tttctaaaga gagtttaatc gcatgttcca aatcatcacc ctcaaaataa gtttgatatg
     3601 caacctgagg tacacctact gttgcaagga cttgttttgt tgtaatttta tccatagcca
     3661 cgcttgaaga tagaatatta gtcccaacat aaggcatcct taaaacttct aaaaatcctt
     3721 ggatagaacc atcttccccc attggtccat gtaaaacggg gaaaacaatt gcattatcat
     3781 catagatatc acttggacga accattttgt ctaaatcaac agtttggttt gtcattaact
     3841 tttcatctga agatggcatt tcatcaaatt cttgtgtttt aataaattga cctacttgcg
     3901 tgataaaata agttttaaca aaaaatttat cataattaat agcacgcatg acgctttctg
     3961 cagacagtac agatacttca cgttccgcag agcgaccacc gtaaagcaag atcaatgttt
     4021 cttttgacat atttcttctc ttttctatct aatctaagat tgacctttta ataataccat
     4081 attttaactt aactttcaaa aatcaatcca cttaaagtta gtattgtaaa agaaaaagtt
     4141 aggagattcc caaccttttc tttataacaa ttataactct gtacgatttt cgattgctcg
     4201 aagcaaggtt acttcatctg catattcgat atctgaacca acagctaaac ctctcgctaa
     4261 tcgtgtcact ttgataccag ctggttttaa gactcgtgaa atatacattg atgtagcttc
     4321 accatcagca gtcgcattgg tagcaacaat aacttctgtc acctgaccat ccatcaatcg
     4381 agtaattaaa cttttcaaat taatgtcatc agggctaata ccattcatag gtgaaattaa
     4441 gccatgtaag acatgataca aaccattata ctcttgaatt ttttccatag ctgacacatc
     4501 tttgctatct tcaacaacca agataacaga ctggtcacgc gtcttatctg tacaaattaa
     4561 gcaaggatca tcatcagtca aattaccaca aacagaacaa taggttaatt cccgcttagc
     4621 agctaataaa ttcttagcaa attcattaac atcttcatca ctcataccaa tggtgtaaaa
     4681 agctaaacgt gttgctgttt tagtacctat acccggtaat tttgaaaagc tgtcaatcaa
     4741 tttagcaatt ggtgttgggt aaagcattca aatgtcctct cttattgtcc tgtgaactgt
     4801 gaaatatatt gatcaatcat atctcgtgca actaattgat gggattttgt tgtatcagtt
     4861 gtaacatgcg gatacattac cgctactgct attttacgat tagtatcgta ggcaatagcg
     4921 tttaagttgt aggtagaaac tgtttgtcca tttacatttt tagcaaatgt ttcagcggta
     4981 ccagttttac cactaatggt tgtcacattc cccctcattg acgtaccagt tgcatagcca
     5041 cttccactat tgacaacgtt ataaaaacct tcttgaataa tggctaactc ttgatctgag
     5101 atagctatct tatttagtgt tttaggagtg attgaacgaa ccaattgagc gaacttatta
     5161 gaatcattcc cttcatagat atctgaaacc acgtgaggtg ctaaacgatt cccattattc
     5221 gcaatagttg agatatactg accaagttgc attggtgtat aggcgtcata ctgaccgaac
     5281 gattccatta gagttgttcc taagctgtat tttccaggta cataaccttc tgattcaggt
     5341 aaatctaatc ctgtagaaac ccctaaacca tattcgccgt aagttttcct tagtttagcc
     5401 attgcttctt gatagcctcg atcagttaaa gcatctcctg tattatagtc ttgtcccatt
     5461 aaacgaagtg ctacttgaac catataagta ttagatgaat actctagcgc ttgtgccgca
     5521 gatattggag tcaaacctcg tgtaaaccat gaccgtatat tagcaatttc ttggtcataa
     5581 agcacttcat ttcctctaag aactttattt tcccatcctg atgatagcgt cgcacctttc
     5641 acaacagatc ctggcgtgaa attcttagca atagtgccta ggctgtcatc aactaaattc
     5701 ccatgatcat ttttcagacc agccattgct aacactttac cagttgaagg ttctatagca
     5761 actgcataca tcccttcgga aaaactagcg cgcccactag ataattcact gccatagtat
     5821 tgtttcaaga tactttctac tttgttttgg taattaagat caatcgttaa tttaagattt
     5881 cttcctgact taccttcttg cgtaatatta tctgaaacaa ctttaccttt tttattaaca
     5941 actactttgc gaatttgtct gattccttgt aagtcatctt catattgttt ttcaaggtaa
     6001 gaagttccca ctcggtcatt cagtgaataa ccttttttca aatatttttt aacttcttct
     6061 cttggtaaac ctgccttttc tgtagaaata gtacctaata tatccgataa agaagttcct
     6121 tttttacgac gatgccaatt acttgttaca gaaattccta acagttcctt cttatcagca
     6181 tccagtttct tgatttgatc atcacttaat tcacctgtac ttaatttcac tgaaccaaac
     6241 gttggtgtcg cattcatttg gttaaataag gcaaccactt ttaactcatc ttcagaatag
     6301 ttaatagcgc tcactggaac tgcagcaact gcatttgcat atacggttga ttcggctaga
     6361 tggttaccaa atttatcgta acgtttagaa tctggtaaac tttcaacaac ttttttgtag
     6421 ttggctttat ctgctaaata ataatctctc tttgctctat cgcttgaagc aacttcggtt
     6481 aaggtaatgt aatgtgataa tttattcgct aactctttga tagactgaga tgaaatattg
     6541 tttcctctag aaaacgccac agtaggacgt tcatcatttt caactaaggc aactccctta
     6601 gcatcaaaaa tctgacctct cgcttttgat tctttcactg tataactcgt aagagctgtc
     6661 agttttttca tgtaaaatga ttgttgccca atctgcattt gctctaaacg aagaattaga
     6721 acagtaaata ataaaacaat aataaaaaat agaagattta atcgtctagg tatgctagca
     6781 ttttgctttt ttacagtgag acgatacctt tttttacgat tcaacattaa ctccttgcta
     6841 taaaaactct gactactatt gtatcataaa aatggttttt tacagacact caaaatgtta
     6901 taaataataa aaaattcaag ccaacttggc ctgaattttt tatataaaga ttagattatt
     6961 tacctaagta atattctgaa acaaggttta atttttcatc aaattcgaaa acaagtggtg
     7021 ggaagttagg aatttcaacg tccatgattt catcatctga caattgtttg atatgtttta
     7081 caagagcacg gattgagtta ccgtgtgcac caacaaacac atttttacca tctttaagag
     7141 caggagcaat tttatcttcc cagaaaggaa gagcacgctc taaagtaact tttaagtttt
     7201 ctgcatctgg aataacagaa tcatctagtg aagcatagcg acgatcagta tgtgctgaat
     7261 gttcatcatc tttagccata tctggaggca atacatcata tgaacgacgc caaatatgaa
     7321 cttgctcatc accaaattgt tcagctgctt ctgctttatt ttttcctgtc aatccaccgt
     7381 aatgacgttc gttcaagcgc catgattttt caactggtac ccaaagttga tcagctgctt
     7441 caagggcaag gttagttgtt ttgatggcac gtttaagaac tgatgtaaaa gcaaggtcga
     7501 actcaatacc tgctgcttgg attaatttcc cagcatcaat agcttgttgt gtaccttttt
     7561 ctgaaagatc tacgtcagcc catccagtga aaaggttagc tttattccac tcagattcac
     7621 cgtggcgtgc gaatactaat tttaccatta gaaaatttct cctttaattt tggctatacc
     7681 aatatttact ttaactattc tacaaaaaat agccataaaa atctagtctg attactagtt
     7741 caataatcaa aaatagctta ttattaacta tctataagaa aaggcatatc acctagagtg
     7801 ctatgccttt agatatcctg tgatttattt aacaaaatct aataaggcaa ggaagctttc
     7861 tgcttcaagt gaagctccac ctacaagggc accgtcaacg tctgggcaag ccatgtattc
     7921 tgcaacgttt tcaggtttaa ctgaaccacc gtattgaacg cgaactttgt cagcaactgc
     7981 ttgaccgaag tctgctgcaa caacgtcacg aactgcttta cacatgtttt gtgcatcgtc
     8041 ttgagttgct gatttaccag taccaatagc ccagattggt tcgtaagcga taacaagtga
     8101 tgatacttgc tcttctgaca aacctgcaag agctgctgaa acttgagcac ctacgaattc
     8161 aaccgcttta ccagcttcgt aagtttcaag agactcaccg caacaaatga ttggagtcaa
     8221 accattagca aagatagctt ttgctttttt gttgatatct tggtcagttt catggaaata
     8281 atcacgacgt tctgagtgac cgataacaac gtagtctgta cccatttcag ccaatacttt
     8341 agggctgttt tcaccagtga atgcacctga attctcaaaa tagctatttt gagcagcgat
     8401 tttcaattct gaacctttag ctgcttcaag aactgttgat aaagtaagcg ctggagcagc
     8461 aatccctgct tcaacaagtt ctgatgatgg taatttagaa gctactgctt cgatgaatgc
     8521 ttttgcttct tctggatttt tattcatttt ccagttacca gcgataaatg gtttacgtga
     8581 cattacacat acctcttctt tttttattta ctaatctatc ataccataaa ttatagttaa
     8641 ttaaaagttt cacgagtcta gtaaaacgct ttaattatag atactaaaaa agagtccctg
     8701 aggactcttc acaatttgag ctgtctgact ctaactgccg tttgggaact taattaagtt
     8761 aaattaagct tcgatttctg aaacgatacc tgaaccaaca gtacgtccac cttcacggat
     8821 tgagaaagta gtaccttgtt ctacggcgat tgggtgaatc aattcaactt cgatagtaac
     8881 gttatcacca ggcataacca tttctgttcc tgctggaagt tcgattgaac ctgttacgtc
     8941 agttgtacgg aagtagaatt gtggacggta gttgttgaag aatggagtat gacgtccacc
     9001 ttcttcttta gaaaggatgt aaacttcacc tttaaattta gtgtgtgggt tgattgaacc
     9061 tggtttagca agaacttgac cacgttcgat ttcatcacgt tgcacaccac gaagaagaac
     9121 accaacgttg tcccctgcaa gaccttcgtc aagttgttta cggaacattt caacaccagt
     9181 aacaactgct ttttggatat cttctttaat accaacgatt tcaacttcgt cgttgacacg
     9241 aacagtacca cggtcgatac gtcctgaagc aactgtacca cgtccagtga ttgagaatac
     9301 atcttcgact ggaagaagta aaggtttgtc agtatcacgt tctggttctg gaatgtactc
     9361 atcaacagtg ctcatcaatt ccatgatgat gtcttcgtat ttttcatcgc cttcaagtgc
     9421 tttaagagct gaaccttgga taactggaag gtcatcacct gggaagtcgt attctgaaag
     9481 aaggtcacga atttccattt caaccaattc aagcaattct tcatcatcaa caaggtcaac
     9541 tttgttcatg aatacgataa ggtgtttaac accaacttga cgtgaaagaa ggatgtgctc
     9601 acgagtttgt ggcattggtc catcagttga agcaactaca aggatagctc cgtccatttg
     9661 agcagcacca gtgatcatgt ttttaacgta gtccgcgtgt cctggagcgt cgatgtgggc
     9721 atagtgacgt ttttcagttt cgtactcaac gtgtgcagtg ttgattgtga ttccgcgttc
     9781 gcgctcttct ggagcagcat cgatagaagc gtaatctttt ggttggttaa ctgaagtagg
     9841 caagcgacgt gccaatacag ttgtgatagc tgctgttaaa gtagttttac cgtggtcaac
     9901 gtgtccgatt gtaccaatgt taacgtgtgg tttactacga tcgtattttt cttttgccat
     9961 tttaggaaaa gcctccaata aaatatattt tatagataga cggtaggcaa tacagtctaa
    10021 ctttacttat ctattttaac aaaatcctct aaaaaagcaa gtattttatg cttttttgct
    10081 tatttcttta tttccttaaa attcgggtaa gattgattga ggcatttgtt atctcttata
    10141 tgtatttttt agtagttatc atataatcat attgttaaat acatcctaat ttagaatatt
    10201 aatagacact tcttttcttc aaatatcttc tctctattaa ttagagttta aatttcaaaa
    10261 aatacaaaaa tcgagataaa aatctcgatt tttgtttgga tgattattta aaggcatcaa
    10321 ggttgattat tttttcattt ttttcttgtg gcttatattg ttcttctgct tctttcatta
    10381 tgagttcctt tttctcgtta gcatcaatat taagcacgaa tccgatagct acagaaagta
    10441 ccaagaggct gttccctcct tgggataaga aaggaaatgt cactccagtt gaggggatta
    10501 acccagaaat accaccaatg ttaacaaaaa cttgcatcaa tagcattgct ccaataccaa
    10561 gagcgatcat tgagttgaaa ggatctttag cctttatacc tacgtgcata atacgtaaaa
    10621 ttaagaagaa aaccaaagct aaaataaacc ctgcaccaat aactcctaat tcctcaataa
    10681 caattgaaaa gacaaagtct gttgttgctt ctggtaagta ccctaatttt tcaatagaat
    10741 tcccaagtcc tcgaccaaac cagccaccgt tgctcattgc ataatatgaa ttagccaact
    10801 ggtgtcctga atctgttaaa tctttaaatg ggtcaaaaaa agcattaaat cgcttagcta
    10861 cataaccaaa gacagggact ttagccattg tctcaactcc aacaactgca atagttccga
    10921 taaataaagt tgaccccacg acgattagac caagtaaagc tgaaaaccat cgataaccaa
    10981 taccactaat acagtacata ataatgaccg ttagaacaat tatcgaacca tttcctaaat
    11041 caggttgtgc aattaccaaa ccaatcataa ataaagaata aaatcgccaa tcttttaggt
    11101 cgcttagtga tctcggaagc catctccctt ttgtcaaagc ttgataatca tatatttcta
    11161 tctttttttg ccttcgagca aaagtaaacg ctaaatacca tacaatgata acttttaaat
    11221 attcagcagg ttgaaaacta ataggaccaa taacaatcca accatgtgct ccattaactt
    11281 cttgtgtgaa aaagcgcgct attagtagta aaaatacttc taccaatact gccatagtca
    11341 acactttaga atttttca
//
LOCUS       DATRTN010000056        11265 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.056, whole genome shotgun sequence.
ACCESSION   DATRTN010000056 DATRTN010000000
VERSION     DATRTN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11265
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.056"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..190
                     /locus_tag="VBE22_001405"
     CDS             <1..190
                     /locus_tag="VBE22_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7202862.1"
                     /translation="PYDNAVAESTYRAFKIEFVYQETFQLLEELALKTKDYVHWWNYH
                     RIHGSLNYQTPMTKRLIA"
     gene            414..1546
                     /locus_tag="VBE22_001406"
                     /pseudo
     CDS             414..1546
                     /locus_tag="VBE22_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1586..1825
                     /locus_tag="VBE22_001407"
                     /pseudo
     CDS             1586..1825
                     /locus_tag="VBE22_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2024..4492)
                     /locus_tag="VBE22_001408"
     CDS             complement(2024..4492)
                     /locus_tag="VBE22_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO7202863.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYRFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDNDSGSEHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YMMPKDGKDYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATVKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNTGDATDTDKPKEKQQAD
                     KSNENQQPSEASKEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQPE
                     GVQFYNKNGELVTYDIKTLQQINP"
     gene            complement(4505..5425)
                     /gene="lmb"
                     /locus_tag="VBE22_001409"
     CDS             complement(4505..5425)
                     /gene="lmb"
                     /locus_tag="VBE22_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEO7202864.1"
                     /translation="MKKGFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
     gene            complement(5661..9113)
                     /gene="scpA/B"
                     /locus_tag="VBE22_001410"
     CDS             complement(5661..9113)
                     /gene="scpA/B"
                     /locus_tag="VBE22_001410"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEO7202865.1"
                     /translation="MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQT
                     VETPQPTAVSEEAPSSKETKTPQTPSDAGETVADDANDLAPQAPAKTADTPATSKATI
                     RDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKAKARYQSKEDLEKAK
                     KEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEGA
                     MPEAQLLLMRVEIVNGLADYARNYAQAIRDAINLGAKVINMSFGNAALAYANLPDETK
                     KAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSPD
                     KQLTETATVKTADQQDKEMPVLSTNRFEPNKAYDYAYANRGTKEDDFKDVKGKIALIE
                     RGDIDFKDKIAKAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDNP
                     QKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKL
                     SGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYFS
                     PRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYY
                     QATVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMKN
                     GYFLEGFVRFKQDPKKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDA
                     KDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTF
                     AKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGNV
                     VWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISS
                     GAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPVYRERIAYTYMDED
                     LPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEG
                     HSNKPEQDGSDQAPDKKPEAKPEQDGSGQTPDKKTETKPEKDSSGQTPGKTPQKGQPS
                     RTLEKRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLVAHIFKTKRQK
                     ETKK"
     gene            complement(9650..10807)
                     /locus_tag="VBE22_001411"
     CDS             complement(9650..10807)
                     /locus_tag="VBE22_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISLre2 family transposase"
                     /protein_id="HEO7202866.1"
                     /translation="MTFSRSRWTNGFETRIPVDEWLGLEKYKRYSIEFLYHVAKLATM
                     MPYRQVCKVIDSTLQTIITKDCVLKAVKFVEKLLKEKERYRFYLEEPPERKKVKKLYV
                     EGDGVMIKSTDSREERRYLDLTHFVIHTGSKKVSTKRYELQDKHEILQLNYDKAKYNL
                     LDYIYNNYEVDDDTILITNSDMGKGYTSRVFKELGKALKVKKHEHFWDIYHVKEKLSS
                     YLRKYPIELTDFALDAVKKYNSDKLELVFDTVESLICDELEDQEFQKFKKKVLNNFKY
                     IKPAHLRNLSNRGIGIMESQHRKITYRMKRRGMYWSKWGISTMANMIIFERANGLREL
                     FFGSWRKVYSEYKEGSFSAGRLFKKTDELDKFSKPLLKNGRKWSITGIKTK"
     gene            10915..11265
                     /locus_tag="VBE22_001412"
                     /pseudo
     CDS             10915..11265
                     /locus_tag="VBE22_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3204 a         2213 c         2063 g         3785 t
ORIGIN      
        1 tccttatgac aatgccgtag ctgaaagtac gtatcgtgct ttcaaaattg aatttgttta
       61 tcaagaaacc tttcaattgc tggaagaact agctcttaag actaaagact atgttcattg
      121 gtggaattac caccgcattc atggtagtct caactaccaa acaccaatga ctaaaagatt
      181 aattgcctag aaaaacactt tataaatgtt gtacagaaaa gtgttgcctt ttcacttaat
      241 aaaagaaaga ttgaaggaac acttctcttt cattccgacc aagggtcaca atttaagtcc
      301 ggggaattta gaaaaataat tgatgacaac aataggcact gaatccctaa aatgagacaa
      361 aagaaaaaca ctcctgttga aatttagtaa actagaaaca ggaatgtttt attatggtta
      421 aaaaagcata ttcatgggaa actaagctgg cttgcatcga tatgaagaaa gcaggcaagt
      481 ctaacagagt tattatggag actttaggca tcaaaaataa tagtcaaatc tatacttgga
      541 tgaaatggta cgaaaacgag gagctttatc gttttcatca aggtgttggc aagcaatata
      601 cctacggtaa aggcttggaa catctgtctg aagtggaaca attacaatta caggttgacc
      661 tcttaaaaaa gtatcgaggc ttgataagga aatcgataaa gtaagtctta tcaagcttgt
      721 ggaagactat aaaaacaact acccgatagt tttcattcta gactgtttta aggtcaagcg
      781 gtcgacctat taccgttgga aaaaggagta tgagaagccg caaaaaaaga tgaccttatt
      841 gaattaattg aacagctctg tatggagaat cactttattt atggctatcg taccattaca
      901 cgattgctca aaaaaatcca cggattgact gttaatacaa agaaagtcta tcgtatcatg
      961 aaaaataatg gctggctttg tcggacacgt accaagaaag ttcccaattt gggtaaagct
     1021 tattatttaa cagataataa attgagtcgg gatttccatg ccgataagcc taaggaaaag
     1081 ctcgtaacag atattaccta cctatatttc ggtaactgta aattgtatct ctcatcaatt
     1141 atgaatctct ataaccgaga aattatagct tacactatct cagattgtca ggacacggat
     1201 tttgtactag acacccttaa tcagttaaaa ttgcctaaat aaaggagcaa tcttacacag
     1261 tgaccaaggc tcagtgtata cttctatggc ttactaccag gcttgcacgg aaaaagacat
     1321 tatccgctca atgtctcgaa aaggaacacc tgcagataat gcctgtattg aatggtttca
     1381 caccgtccta aagactgaaa ccttttattt ccataatagg agaaaataca acaaagatag
     1441 tataacaaat attgttaaaa attacattac attttataat gaaactagaa ttcaacagag
     1501 attaaacgac cagtcgcctg tacagtacag aaaactgatt gcctaaaagt gtttttcttg
     1561 cgtctcacta ttagggtgca gtgccaatat catgcattct ttttctaatc ctggatatcc
     1621 ttatgataat gccgtaactg aagcattttt caagtattta aagcatagac aaatcaaccg
     1681 aaaacattat caaaatatca aacaggttca attagactgc tttgaataca ttgagaattt
     1741 ttataacaat tacaacccac atacggctaa tctaggacta acccctaatc agaaagaaga
     1801 aaattatttt aacgcaataa aataacacag ttttctgtct acttatttga cattagtcca
     1861 agaatcctcc tcttttttta ctattctaac atggatacca tttctttgca aaagaaaacc
     1921 gctattatcc aacttttatc actactcgtt acatagaatg acttcaaaat caccgtaact
     1981 tgctttgaca aagcagtgct ttaacaatga gatcttcttt tggttaaggg tttatttgtt
     2041 gaagtgtctt gatatcataa gttaccaatt caccattttt attataaaat tggacacctt
     2101 ctggttggaa aatgagatac ttaggatcga tattagcttt ttgtgctaat tgattgatat
     2161 gatcttctag ggttgctcta tctagaccat agtctggtaa actgtctata aagtcatctg
     2221 attctttttc ttctttactg gcttcacttg gctgttggtt ttcattgctc ttatctgcct
     2281 gttgcttttc tttgggttta tccgtatcag tagcatcacc agtatttttc tttgccaata
     2341 actcttgagc ttgttgccac tcagcttggg atagatcaga tttattaatg gttctcaatg
     2401 aactgccatc tgctcttggg atgctaaagg agccatcttt ccatacaaaa gtttctttgg
     2461 ccaaaacatc tcgtggatcg aaaatatagc cgtctggtgt tgcaaaacga ccttctgcta
     2521 gggctttttg aacttcttca gcagaatgga taatttgcca gtttggcata ccagcacgtt
     2581 tatcaagagg cgtaacattt ggaatgaccg aacctgactc ttcatgccct ggcttagacc
     2641 atacatccgg acgaacttcg gggtgttgca tcacatactt gactgttgca atctgatcgc
     2701 gcgtcaacca tgaatacgga acgacatgga tatgatcaat atgtgggata acaaacgaac
     2761 ttccagtatc gtaagtagca ttaccagcat gcatcggcag acttgacaca tctacagcaa
     2821 gtcgtggctc aatacctgtg tcaacaatgt cataacggta atgcttctta tctttaagca
     2881 ttagttcttg ttcggcaaag gcaatctgag tcaaatcaag ttgatcacga gcatagaaat
     2941 agtccttacc atcttttggc atcatatagc ccactttacc atcttttgtt actttgcgac
     3001 tcactttttt agtgtcaaag agtggttttt ctttgccttg ttcctgatcc aaagcagcag
     3061 caagctcatc agcttgacct tttgctttca cccagttagc gacctcatcc aactcatatt
     3121 gttcaagttc tccaaatcct atatagtgga aatgatctcc gtgtttagct gtaactcctg
     3181 atttatccac tgaatgaatg gattctttac taaaaacata agcatcactc gtatcatatg
     3241 gtttaccatc taagcctttt ccgtaagctt tgatgcgatg accaagaaag gtatgtgtca
     3301 cttctttatc tgatggtttt gagtgctctg aacctgagtc attgtcctca gtttggccag
     3361 ctaagtatcg atctgctaat tccatttcaa gaggtgataa ctgacttctt gggataatat
     3421 gataatgatc tccatgaggc accacatacc caaaagcgtt tgatttgatc acttgagtcg
     3481 gttcaaaaat caacccatct tcttccacat gacggtattt caaatcaaga cggtgtagtt
     3541 gatctaaaag ttccttaaag gtttttcctt taaactcatc tctttggtgt ttgttttgtg
     3601 acgcatcatt tggcctagga ggcgctggat gatagccacc gttatctggc tgatgacctt
     3661 gtccagggtt aggcgtcaca tcaggaattg gggctttcct acgacctggg gctggtgtcg
     3721 ggcggtaatc agacggtcta gcacctcgac cttgtttttg actccagtag gcttgtgcag
     3781 cagctagctc acttggagac aaatcctttt taggaatata atgatagtga ttaccatgag
     3841 gtactaaata agcatctcct aaatcatcaa tgatatctgt cggactaaaa atatagccat
     3901 cgtctgtagt atagcgtcct tgtctttttg cttcattgac tgccgcaact tcttctttac
     3961 tgagatgggc cacttgagct aaaccttttt ctttagcttc tttagttcct ttggctactt
     4021 gctcagcaat ttgttgtttg gttcgaatgt ttttgcgctt actacctggc ttgaggtaaa
     4081 cataatagtt gccattgact ttaataacgt aaccgtctaa gatttcattg ataacgtctg
     4141 attgtttaaa acggtaatta ggatccgtca tcaacaactc ttcactaata atcgcatcat
     4201 aaggaacttt cccattgtaa aaatgataat ggtcaccgtg tgaggtcaca tagccttggt
     4261 cagtaatttt gactacgatc tgttcagcag agatgccttc ttcagcactg atttgatcca
     4321 tcgttttgtt tgttttaggg gcttttgcct tacctttgct gtcatcaata taggcaatct
     4381 gattgtcctt tgttgctaga cccatatgat gcttaccaag ttggtaactt ccaatatgag
     4441 tagctagtaa aatggcagca actgagccga tataaccata tgttttcttc actaataatc
     4501 tcctttactt caactgttga tagagcactt ccaaatttgc tctaagattt tctagatatg
     4561 tcttgtttcc gcttggagca gcttcaagtg gacttaatgt ctttacttta gctcctgttg
     4621 atttcgcaat agcatgagca attttgggat tgacgttgtc ttctgcaaaa atagtcttga
     4681 cgttgtattc tttgacaaag tcttgaattt ctttcaattg gcgaggagag ggctcttgct
     4741 ctggagaaat acccgagata ccaagttgtt tcaagccgaa tcgtttagcc agataagaaa
     4801 atgccgtgtg ttgcgtcacg aatgtttttg agcgcacctt tttaaatttt tgagtgtatt
     4861 cttcagttag ttgctctgct tcttttttga aagccttagc ctttttagtg taactgtctt
     4921 tgtgtttagg atccaaacgt cctagctctt tagcgatatt aacagcttcc tcaccagcta
     4981 aaacgggatc cgtccaggta tgtgggtcat aaagtgtcgc agggtcaatg ccttgtgtga
     5041 cttccatatc ttctagccct ttgactctat ctagtgtcaa aggttttgac gcttcaaaca
     5101 catcaacctt tgattttttt aaattagggt ctagatccct tgcccaagct tctaaggtat
     5161 gtgaatggta aacaaacaaa tccgcgtcat aaatagctgc cacatcattt acagacggtt
     5221 caaaggaatg aatgcctgca cctgattgga tcatcctcac atcattgagg tctccagata
     5281 cttcttttgt catcgcatac attgggtaaa agctggttac aactgacatg ccttgcgtag
     5341 gctgtttggg gtttgctgac ttatcacacc ctgctatcat tactaaactc acaaccatag
     5401 ccatgagaaa aaaacctttt ttcatagtac ctcctcaatt ataatttaac cagttattaa
     5461 ccagttaatt ttactccttt atcaatgctt tgtcaatggc aactaccatc ttttatcaca
     5521 tcaactgaag ggataagcaa aaggcaattg ttgacagcta tttgtcctaa cttctttttg
     5581 atgaggtagc acatataaaa aaatagagag aaccaaattg gttctctcta ttaaataagc
     5641 tcttatcagt ttaagtaata ctatttttta gtttcttttt ggcgttttgt tttaaatata
     5701 tgagctacta atcccaagaa gaaagtggtc ataactaact taaggagatg taaacgattt
     5761 gtatccttgt cattagtcgt tggtaactga tctcttgttg atgcttttgt agctaaagca
     5821 cgcttagaag atcgtttctc tagagtacga gaaggttgac ctttttgagg agttttacct
     5881 ggtgtttgac ctgaactatc tttttctggt ttagtttctg tttttttatc tggtgtttga
     5941 cctgaaccgt cttgttctgg tttagcttct ggttttttgt ctggtgcttg atctgaaccg
     6001 tcttgttctg gcttattaga gtggccctcc aatagcttag tcactggtgt ataagtgatg
     6061 ttaccagcca tatcttcaac aacataagta aagtctgaca ttttcaatgg aacagtagcg
     6121 ccttccattg tttcagcctc ttcaggaaga gtaaaggtac catcttcatt tggagaaata
     6181 tactctgttg ttggcagatc ctcatccatg taagtataag caatacgctc acggtaaacc
     6241 ggttggctgg tttttggttt agatgcaagt gtcaaacgac gatcttctgt tgagaatgtt
     6301 gccgatgttg cgacttcagg tgtcgtattg tctacaatca catcaaaatc agtgtgttgt
     6361 tcttttgcac ctgagctaat cggagtgtag cggacacgat aggtgtaggt tccgttagca
     6421 acaactttgc cgtctttatc tttaccgtcc caacgcgttt tttcaaaacg ggttgaacca
     6481 agtgtgcttg ccaagtcatt gttgtagttt ttaacaactt gctcggttac ctcacttgtc
     6541 caaacaacat ttccttcttt gtccaagact tcagccacaa ggtttttagc attacgcaag
     6601 aaagtacctt ggaattggac ataatctctg ttaccgtccc catttggaga gatcgcagca
     6661 tatggcttgc cattagcgtg acggtggata tagtagtggc tatcatcgtc ttgttttgca
     6721 aaagtacctg caaaaatggt ttctgtgatc tctgaagatt cgatatcctc tatgttttca
     6781 accccttctt tgacagcttt aataatcgtc cacgggttag actctgtggt aagtgctgta
     6841 aagttatttt tcagagcgta aaactgtaat ccatcaccat ctaattggtc tttggcatca
     6901 ctatttgctt catgatagta gctgctaccg tctttgctat catagattgg tttttctaag
     6961 gctgacagat tgccaaaatc acctcggaaa ccaatatatg gaatgctcat aagctcttct
     7021 tttttaggat cttgtttgaa acgaacaaaa ccttctaaga aatagccatt tttcatttgg
     7081 gcaagcaagt ccttgctaaa tcgactagca tcgattggaa cggtgacttg tttgctgcta
     7141 ttggctggaa ttgtgatttt ttgccatgat gtctcataca atgctttagg agccaaggca
     7201 aagtgttttc catctacttt atctgtttga acagttgctt ggtaatacaa ctcttgaggt
     7261 ttatcagatt tgttgtgaac tgttactgtt acttcaaatt tatcagaaac attgttcagg
     7321 tgaaccttgc ttgaggtatt atccttatct gtcacataca tcgttgctgc tgaagctttt
     7381 ttagcatcga ctgctcctgc tccttgttgg cgaggagaaa aataagcttt ttcatcttca
     7441 tcatataagg cagttgctga gctcatcaat acttttttag ctaaatcaag acgctctgat
     7501 ggtgtcatat caggatactg tgtctcatac tgcttttgca acagtcccat gatacccgct
     7561 actaatggcg cagacatact agttccagaa agtttggcat acttgttgtt agccactgat
     7621 gacaaaatat cttggccggg tgctgcaata tctggcttaa tattaccgtc agctgtcaga
     7681 ccccagcttg agaagcggct tagtttggtg ccacttgctg ttggcaatac cttaggtgtc
     7741 gcattgaagg tgatggtttt ttggggattg tcttttaata agagaccatc ttttcgactg
     7801 ataaaggccg caggcatctg atcaacattt ggcaattcaa tcgggaagcc cttgtcctga
     7861 ttgtcataga tcaagactcc tacagcacca gcttttttag cttttgcaat cttatctttg
     7921 aaatcaatat cgccacgttc aataagggca atcttacctt tgacatcctt aaaatcatcc
     7981 tctttcgtcc ctcgattagc ataagcatag tcgtaagcct tgtttggctc aaaacggttt
     8041 gttgaaagaa caggcatttc tttatcttgc tgatcggctg ttttgaccgt agcagtttca
     8101 gtgagctgtt tatctgggct gtaagaagca actgtcaatg ttgagtctgc cgctgcaggt
     8161 gtcccaacca ccccataatc aggatgatct gctagaggta gacgggtctt gcccccaaag
     8221 ctactatcat taccagctga ggtcacaatg ctaacacctt ttgatttggc atagtcaaag
     8281 gcttttttgg tttcgtctgg aaggttggcg taagctagtg cagcattacc aaagctcata
     8341 ttaatcacct tagctcccaa gttgatagca tctctgatag cttgagcgta gttacgagca
     8401 tagtctgcta gtccatttac aatttcgaca cgcatcaaaa gcaattgagc ctcaggcatc
     8461 gcaccttcta ggcggtaagg ttctttcgtt tcagatggag catttcctga caagattcct
     8521 gacacgtgtg tgccgtgctc ttgatcgaca gcggttttac catctttact atagtcatgg
     8581 tagtaagcaa ccttatcatt gacccactcg ccataggtaa taccgtgctc ttttttagct
     8641 ttttcaagat cttcttttga ttggtaacgt gctttggctt tgtctgttaa gcgccacgct
     8701 tcatgatttt tatcaaaacc agcatcaatc actgcaacaa cagtcccagc tcccttgcct
     8761 gctttttcct gcagggtttt gacctgagaa gggtcgttca aatccctaat agtcgctttt
     8821 gaggttgctg gtgtatcagc agttttagca ggagcttgag gggctagatc attagcgtca
     8881 tctgctactg tttctcctgc atcactagga gtttgtgggg ttttagtttc ctttgatgat
     8941 ggtgcttcct cagaaaccgc tgttggttgt ggagtttcta cggtttgttc ggtagcagga
     9001 gtgtcttctg tcacagtatt tgctttgatg tctgattgtg cattgagcaa gatgctcgta
     9061 gacataagcg caatggcaag tttatcaaat ggtagtttct gttttttacg caatgtgtcg
     9121 tcctttcatg atgagagact ttgtctttat gaccattaag aaaagagaga atcaaacgat
     9181 tctctcctag ttatccatct gtgcgtggag aaataaggat aacgtctttt ttaaagagca
     9241 aacaggaggt tatttgcata tgatacagct atttttcttg cttttgtcat aatgattaaa
     9301 tgtatctcat ttattaatta tattactaac atatttatta taaaacaaag attattatta
     9361 gtaaaggtca gctttttaag agttgcttat tttgtgacct ccttaatctc attattctat
     9421 cataaaagag agaaccgatc ggttctctct tgatgttcac tcaggtatgt gtctgaaatt
     9481 ttgagattag tcgatacctc ataaatttaa gaaaacttga attattctta aaaagaccaa
     9541 aacaagtatt ttttgctttt ctttaatata attatactac tttagaagat ttaatgcaag
     9601 ttaaaacatg caaaaaaacg ctaaaaataa aggtattttt agactttttc tattttgttt
     9661 tgattcctgt tatactccat tttctgccat tttttagaag gggcttagaa aatttatcta
     9721 attcatctgt ctttttaaaa agtcgccctg cactaaatga accttcttta tactcactgt
     9781 atacctttct ccaagaaccg aaaaataatt ctcgtaaacc gttagctctt tcaaatataa
     9841 tcatatttgc cattgtggag attccccact ttgaccaata catgccacgt cgcttcattc
     9901 tatacgttat ctttctgtgt tgtgattcca tgataccaat accacgattt gaaagatttc
     9961 taagatgagc tggttttata tatttgaaat tatttaatac ttttttctta aacttctgaa
    10021 attcttgatc ttcaagttca tcacaaatca gtgattcaac agtatcaaaa actaattcaa
    10081 gcttatcaga attatatttt tttaccgcat ctaaagcaaa atcggttaat tcaattggat
    10141 attttctaag gtatgaactt aacttttctt taacatgata gatatcccaa aaatgctcat
    10201 gtttctttac cttaagtgct tttcctaatt ccttaaaaac tctactagta tagcctttac
    10261 ccatatcaga gttagtgatt aaaatagtat cgtcatctac ttcatagtta ttataaatat
    10321 aatctaaaag attatattta gctttatcat aattaagctg taatatttcg tgcttgtcct
    10381 gcaattcata tcttttagta gaaacttttt ttgagcctgt atgaataaca aaatgtgtta
    10441 aatctaaata ccttctttcc tctctagaat ctgtgctttt aatcatgact ccatcaccct
    10501 caacatacag ttttttcact tttttacgtt cgggtggctc ttccaaataa aaacgatagc
    10561 gttctttttc ttttaacaat ttttctacaa attttactgc ttttaaaaca cagtcttttg
    10621 ttatgattgt ttgcaaagtg ctatctatta ctttgcaaac ttgacgataa ggcatcattg
    10681 tagccaattt tgcaacatga tataagaatt ctattgaata tctcttatat ttttcaagac
    10741 ctaaccactc atctactggt attctagttt caaagccatt tgtccagcga ctcctactaa
    10801 atgttatttc tccaaaagag aaaacaaccg tacgttcaga ctgtgaatag gttacactaa
    10861 actagacaga aattataaag tgttctacac ttaaaaaaaa actggagaaa aaccatgtct
    10921 agaaaagtac gtcgtcactt caccgatgat tttaagcaac aaattgttga cctttacaat
    10981 gttggtagaa agcgtagcag tctcatcaag gaatatgagc taaccccttc caccttcgat
    11041 aagtgggtta gacaagccaa aacaactggt tcattcaagt ctgttgataa tctgacagat
    11101 gaacagcggg agctaattga actcagaaaa cacaataaag aactcgaaat gcaattagat
    11161 atcctaaagc aagcagcggt gattatggca caaaaaggga aataatcact gctaataagg
    11221 ataaatacag catttcaaag atgtgtcgct ggctgaatat gccac
//
LOCUS       DATRTN010000057        11151 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.057, whole genome shotgun sequence.
ACCESSION   DATRTN010000057 DATRTN010000000
VERSION     DATRTN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11151
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.057"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            35..625
                     /locus_tag="VBE22_001413"
     CDS             35..625
                     /locus_tag="VBE22_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEO7202867.1"
                     /translation="MANTVGNWTYLLLFLVIFVETGAVIFPFLPGDSLLFAAGALAAN
                     PKMSFNIVTFLIIFFIAAFIGDSCNFLIGRTFGYRFIKHPFFRRFIKEKNIRDAELYF
                     EKKGTAAIILGRYIPIIRTFVPFVAGISQLPPKVFIKRAFIAALSWSVIATGSGFLFG
                     NIPFVKQHFSLIILGIVFVTLIPVLISGVKSYRNTK"
     gene            complement(703..1488)
                     /locus_tag="VBE22_001414"
     CDS             complement(703..1488)
                     /locus_tag="VBE22_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7202868.1"
                     /translation="MKRLHKLFITVIATLGMLGVMTFGLPTQPQNVTPIVHADVNSSV
                     DTSQEFQNNLKNAIGNLPFQYVNGIYELNNNQTNLNADVNVKAYVQNTIDNQQRPSTA
                     NAMLDRTIRQYQNRRDTTLPDANWKPLGWHQVATNDHYGHAVDKGHLIAYALAGNFKG
                     WDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDT
                     DLVPFAMHLEAKSQDGTLEFNVAIPNTQASYTMDYATGEITLN"
     gene            1749..2624
                     /locus_tag="VBE22_001415"
     CDS             1749..2624
                     /locus_tag="VBE22_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEO7202869.1"
                     /translation="MKKTIGIIGMGVSGLAVLLALSRKKEKELKQLAIFCFDDTVHFG
                     RGIPFQEDVDSALINSPINDISFDYRHMSDFMDWLEKNYYDTNLTYVSRTLYGQYLSE
                     RTKLLLDKLPVTVIKTRVDKLNYLSDKQQWQVYIQDKLFPVTFDELHLACGALPVIDP
                     YHLEGKTDYIKDPYPIKHLAQENWKGKTVAIIGTGLAAIDVIKWLLLNTQSSVKAFSR
                     TNYFPSVRIVNDLPLTWHFFTNDNLKAFLSHPSKTFTIADFNDLMSGELEHLGFKNWK
                     NTTETFLSPGIDGLK"
     gene            2709..3194
                     /locus_tag="VBE22_001416"
     CDS             2709..3194
                     /locus_tag="VBE22_001416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202870.1"
                     /translation="MSLSDKKEYKALYGKAIINLRNPMPDESAKVILKAAQKGTFSLI
                     NNVKTIAKNDEGFSIEKEKQNHISVDRVINATGYQLNTKNWTKANPLLKSLINQEFCQ
                     IDDQGGITILTENAQVLSPKYGPMPGLYAHGALVNGVVYQNNSTIKIQQMAERAFSDN
                     K"
     gene            complement(3251..3922)
                     /locus_tag="VBE22_001417"
     CDS             complement(3251..3922)
                     /locus_tag="VBE22_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202871.1"
                     /translation="MFELKNIAYRYKGNDNKTLENINYSFQSGVFYTILGNSDSGKTT
                     LLSLMAGLDSPTEGQVLFNKKDIKEAGYAQHRKKNIALVFQNYNLLDYLTPLENVQLV
                     KPTADKQLLLDLGLKEDMLTRNILRLSGGQQQRVAIARALVAGTPAILLDEPTGNLDF
                     DIARDITMRLKDFAHKEKRCVIMVTHSREIAHMADTALQLTGDNLKELSKESIAQSIL
                     QQRNL"
     gene            complement(4019..4328)
                     /locus_tag="VBE22_001418"
                     /pseudo
     CDS             complement(4019..4328)
                     /locus_tag="VBE22_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
     gene            complement(4571..4831)
                     /locus_tag="VBE22_001419"
                     /pseudo
     CDS             complement(4571..4831)
                     /locus_tag="VBE22_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4885..5359)
                     /locus_tag="VBE22_001420"
                     /pseudo
     CDS             complement(4885..5359)
                     /locus_tag="VBE22_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5369..6004)
                     /locus_tag="VBE22_001421"
     CDS             complement(5369..6004)
                     /locus_tag="VBE22_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7202872.1"
                     /translation="MVRKISKQKGYQTVAVRLSDKRLVDTVIQNIKQWPFDFGKLDVQ
                     TAKEFYGDSYRNIETLHRLVGRIILIVSLVAMAILVVMLTFWINNRIKETGILLAIGK
                     TKFEIIGHYLIEVLLVAGAAFTLSIIGGVFLGKTFAAGLLSQVNGGVSSQIVQNSSLI
                     IDRIDNLTVSVGVMDVFRLYAQGALICLFAVVLSSYSILKLQPKQILSRMS"
     gene            complement(6165..6740)
                     /locus_tag="VBE22_001422"
     CDS             complement(6165..6740)
                     /locus_tag="VBE22_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylK protein"
                     /protein_id="HEO7202873.1"
                     /translation="MNPLSEKDKVNYIMIYKNRQSTLENNLSGDRLMRYQQANRQVSK
                     DKLLTGSYFVYQCLKKLGFTNEICQESFSSVSSYLIGLPKGKISYSNSGDYHILTYAP
                     SGSTGVDIEKYKDRSEQTYQTYLGESVSSDMDSKLLFYKAWLQKEISYKCGKSIDITY
                     QTMIDGYIYGYAFDNTFKVELVYLKELLPDN"
     gene            complement(6709..7920)
                     /locus_tag="VBE22_001423"
     CDS             complement(6709..7920)
                     /locus_tag="VBE22_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylJ protein"
                     /protein_id="HEO7202874.1"
                     /translation="MSKFLFAPFMMNLGESQRLSKLANYLYEQGHDIHILGDNYYPFL
                     FNNDAYRYHHCVEDKSVYNSKRYDAFFSLSTDFNFLSEEEIERICSIERDLLRQEKFD
                     AVLTGYRLSIITSCRLESIPLIWIISGATHISEIVENSEGILPNGKISKASKPQTKDF
                     IKRVITTYSTNVKTWNNYIKKYGGKPFNNALELFTGDLNLVTDYSLFYEFDKDSSYKT
                     IGPILIDNVGFSKCSQIKQDNKTVLLSFGTSFKRDWVESFLKTLPRHYHYLLTSCGEA
                     INIPGSYIEVVDFIDFKTIDKEIYFAIIHGGQGTVYAMAAQGIPFIGIPFFNEQFWNI
                     KKFSKEKCAYLVKKPDVREIRKAISCLERGYDIYKSHMIRLSRGIKEEAEASLQKAEA
                     EIESFIRKRQS"
     gene            complement(7925..10120)
                     /locus_tag="VBE22_001424"
     CDS             complement(7925..10120)
                     /locus_tag="VBE22_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEO7202875.1"
                     /translation="MSVYVSGIGIISSLGKNYSEHKQHLFDLKEGISKHLYKNHDSIL
                     ESYTGSITSDPEVPEQYKDETRNFKFAFTAFEEALASSGVNLKAYHNIAVCLGTSLGG
                     KSAGQNALYQFEEGERQVDASLLEKASVYHIADELMAYHDIVGASYVISTACSASNNA
                     VILGTQLLQDGDCDLAICGGCDELSDISLAGFTSLGAINTEMACQPYSSGKGINLGEG
                     AGFVVLVKDQSLAKYGKIIGGLITSDGYHITAPKPTGEGAAQIAKQLVTQAGIDYSEI
                     DYINGHGTGTQANDKMEKNMYGKFFPTTTLISSTKGQTGHTLGAAGIIELINCLAAIE
                     EQTVPATKNEIGIEGFPENFVYHQKREYPIRNALNFSFAFGGNNSGILLSSLDSPLET
                     LPARENLKMAILSSVASISKNESLSITYEKVASNFNDFEALRFKGARPPKTVNPAQFR
                     KMDDFSKMVAVTTAQALIESNINLKKQDTSKVGIVFTTLSGPVEVVEGIEKQITTEGY
                     AHVSASRFPFTVMNAAAGMLSIIFKITGPLSVISTNSGALDGIQYAKEMMRNDNLDYV
                     ILVSANQWTDMSFMWWQQLNYDSQMFVGSDYCSAQVLSRQALENSPIILGSKQLKYSH
                     KTFTDVMTIFDAALQNLLSDLGLTIKDIKGFVWNERKKAVSSDYDFLANLSEYYNMPN
                     LAFGQFGFSSNGAGEELDYTVNESIEKGYYLVLSYSIFGGISFAIIEKR"
     gene            complement(10117..11070)
                     /locus_tag="VBE22_001425"
     CDS             complement(10117..11070)
                     /locus_tag="VBE22_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEO7202876.1"
                     /translation="MNTIYNTLRTDKGYKVYEGYLYEITGEECEEALDLVIPKNIVFA
                     DTDTCGYTFLLNEDGTVYDDVTFYKFDDKYWLASHKALDSYLDNINFDYTVTDISDEY
                     KMLQIEGRYSGEIAQSFYEYDISTLNFRTLIEMTYKGEKGYLARFGFSGEFGYQFFLP
                     SSIFATFVSDVCEGIAEYGDELDRYLRFEVGQPITDIYQQEEYSLYEIGYSWNLDFTK
                     EEFRGRDSLLEHIRSATVKSVGFSTKEKLASGTPVLFDDQIVGKIFWIADEKDSSENY
                     LGLMIVNQTYAHSGVTFVTEDGQILKTQSSPYCIPESWNKE"
BASE COUNT         3524 a         2124 c         1688 g         3815 t
ORIGIN      
        1 ttttatttta catattgata ctcatatcta tgctatggct aacactgttg gtaattggac
       61 ctatctattg ctcttcctcg ttattttcgt tgaaactggg gctgtcatct ttccattttt
      121 accaggtgat agtttattat ttgctgctgg agcccttgct gcaaatccaa aaatgtcctt
      181 caatattgta actttcctta ttattttctt tattgcagca tttattggtg atagttgcaa
      241 tttcttgatt ggtcgtacat ttggttaccg ttttatcaaa catcctttct ttcgtcgttt
      301 cattaaagaa aaaaatattc gagatgctga gctttatttt gagaaaaaag gtactgctgc
      361 catcatttta gggcgctata tccctattat cagaaccttt gttccttttg tagcaggtat
      421 tagtcagctc cctccaaaag tatttatcaa acgcgccttc attgcagcat tatcttggtc
      481 agttattgca acaggttctg gttttctctt tggtaatatt ccttttgtga agcagcactt
      541 ctcgcttatt atcttgggaa ttgtatttgt taccttaatt cctgtcctta tttctggtgt
      601 caaatcatat cggaacacta agtaatgtat ccaaaaattt ctatttaaca ctattaatca
      661 ctaagaatga caacaaaaaa agagcaatta gttgctcttt tattaattta gtgttatttc
      721 tcctgttgca taatccatag tgtatgatgc ttgtgtgttt ggaatagcaa cattaaattc
      781 taatgtgcca tcttgtgatt tagcttctag gtgcattgca aatggaacta aatcagtatc
      841 attacggtac aatggagtta cacggtaacg aacacgtttg ttttggtcaa ccgccttacg
      901 aactaagctt tcataataat tttgtccacg attgattttt tgatttgatt ggttggaatg
      961 agctgtttgt gtgacaacat tttgaggatt tgacacggaa gcatcccaac ctttgaaatt
     1021 tccagctaaa gcataggcaa ttaaatgccc cttgtcgact gcatgcccat aatggtcatt
     1081 agtagctact tgatgccaac ctaatggttt ccaatttgca tcgggaagag tggtatctct
     1141 gcgattttga tattgacgaa tggttctatc aagcattgca ttagcagttg atggtctttg
     1201 ttgattgtca attgtatttt gaacatacgc tttaacattg acatcagcat ttaaatttgt
     1261 ttgattatta tttaattcat aaataccatt aacatattga aatggtaggt taccaatagc
     1321 attttttaaa ttattttgaa attcctggct cgtatcaaca gatgaattga catcagcatg
     1381 tactatcggt gttacgtttt gcggctgcgt tggaagacca aaggtcatta cccccaacat
     1441 acctaatgta gcaattacag ttataaacag tttatgtaat cttttcataa gctttacctc
     1501 tttctaatta cattaaacaa gcgtctacca tattgatatc cgttttagct aattgctctc
     1561 cttttgtcac aaccatatca tatgcctatt atttaagcat gtcaatcatt tatttaatct
     1621 tctcttaatt taatattact ttaaatttaa tttactcgtt aaattagaaa ttctgcaaat
     1681 aatagctttg ttcatcccct aacttaaatc aaaatttatg ctatgatagt ggtaaggagg
     1741 cttgttctgt gaaaaaaaca attggtatta taggtatggg agttagtggt ttagctgttc
     1801 tactagcact ttctcgtaaa aaagagaaag agttaaaaca gttagctatt ttttgttttg
     1861 atgatactgt ccactttgga cgaggaattc cttttcaaga agacgttgac tcagctttaa
     1921 tcaattcccc aatcaacgat atttcatttg attatcgcca tatgtctgac ttcatggatt
     1981 ggttagaaaa aaattactat gacacaaatc taacctatgt atcaaggaca ctttacggac
     2041 aatacctttc agaaagaact aaattacttt tagataaact ccctgtcacg gttattaaaa
     2101 caagagtgga taaactaaac tatttatcag ataagcaaca atggcaagtc tatattcaag
     2161 ataagctttt tcctgtcaca tttgatgaat tacatttagc ttgtggtgct ctgcccgtta
     2221 tagaccctta tcatttggaa gggaagactg attatatcaa agacccttat cctatcaaac
     2281 acttggctca ggaaaattgg aagggaaaaa cagttgctat catcgggact ggtctagctg
     2341 caattgatgt tataaaatgg ttgctactca atacacaatc ttctgttaaa gcattttcaa
     2401 gaaccaacta cttccctagc gtccgtattg ttaatgattt accacttact tggcactttt
     2461 ttaccaatga taaccttaaa gcatttctat cacatccttc aaaaaccttc actattgccg
     2521 attttaatga tttgatgtct ggtgaattgg agcatcttgg ctttaaaaac tggaaaaata
     2581 caacagaaac ctttttatcc ccaggaattg atggtctcaa gtgagccttt aagtacccta
     2641 agcaactcta tctattacaa caactagctt cacgaatcac tgtctggtta actgaccttt
     2701 ggccactcat gtcattatca gataagaaag agtataaagc actatatgga aaagcgatta
     2761 ttaacttgcg aaatcctatg cctgatgaat ctgctaaagt aattttgaaa gctgcacaaa
     2821 aagggacttt ttccctgatc aataacgtca agacaattgc aaaaaatgac gaaggatttt
     2881 caatagaaaa agaaaaacaa aatcatatat ctgtcgatcg cgtcattaat gctactggtt
     2941 accagttaaa tactaaaaac tggacaaaag ccaatccact cctaaaatcg ctcatcaatc
     3001 aagaattttg tcaaattgac gatcaaggag ggattactat tttaactgaa aatgcacaag
     3061 ttttgtctcc aaaatatggt ccaatgccag gtttatatgc gcatggtgca ctggttaatg
     3121 gtgtcgttta tcagaataac tctacaatta aaattcaaca aatggcagag cgagcctttt
     3181 ctgataataa ataaccattt ctaaaaaata cctcctcttt tattatttaa gaaagggagg
     3241 tattttttta ttataaattc ctttgttgta atatagactg tgctatactt tcttttgata
     3301 attcttttaa gttatctcct gtcaattgta aagcagtatc agccatatga gcgatttctc
     3361 tactatgtgt caccataata acacatcgtt tttctttatg cgcaaaatct ttaagacgca
     3421 tcgtaatatc acgtgcaatg tcaaaatcta aattccccgt aggttcatca agtaaaatag
     3481 ctggtgttcc tgcaaccaaa gccctcgcaa tagctacacg ttgttgttgc cctcctgata
     3541 atctcaagat atttcgcgtt aacatatctt cttttaaacc taaatccaac aataattgtt
     3601 tatctgcagt aggttttacc aactgaacat tttccaatgg ggttagatag tctaaaaggt
     3661 tataattttg aaagaccaaa gctatattct ttttgcgatg ctgagcataa cctgcttctt
     3721 taatatcttt tttgttaaat aatacttgtc cctcagtggg cgaatcaaga cctgccatca
     3781 aggatagaag ggttgtttta cctgaatcag aattccctaa tatagtataa aatacccctg
     3841 actgaaaact ataattgata ttttctaatg ttttattgtc gttcccctta taacgataag
     3901 caatattttt taattcgaac atagatttcc ctttcttgag ctatgtattc aatagcagag
     3961 catattcatt ttaaccgtta atatagatgt cactattggt cggtatactt atggcacttt
     4021 aggacagttt tgataagatt tcccttggtt tgtagcgagc aataattaaa ctagtagtaa
     4081 taatagcaaa taagacgatt gctaaaccaa ctagaattac taaccagact tcctcaggct
     4141 tcacttgtac atttcgtttg cataaacata tctgtatcac tatcagaccc aagctgcata
     4201 ttcccaaacc ctttcataaa gtctgctctg acagttctag aggcctgata cactaaggag
     4261 tctgctaatt tttgaccaat aaattgaccg ctaagtactg ataaagcaaa ggctggaata
     4321 gcaatcatta gtacttctac tgcgtactga caatgataat tgctttagac ttaccaatag
     4381 ctaataaaat acctgtctca tgggttctac caccaatcca gaatagcaga gccatcatca
     4441 ctaggataat agatactact aacatgccgc cttgtagaga agtaattaat tgattcaaga
     4501 catctatact ttttgcataa cttgttagca aggttgctgt aattgtcagt tggtatttat
     4561 tccaatcaat agattgtttt ttgataaaac ttttaagagc ttttatatca gctttctttt
     4621 taggatatac agttgtttgg cgataaaagg ccttaccttt agaatagccg tataaatcct
     4681 taatcaattg cgtatctgaa aaggctgtat tttctagcat ctcataaggg taatttgatt
     4741 tttgtaccgt tttaccttta tatatgccaa caatagttgc ttcaactaat gttttatcct
     4801 tactgtttct aacggggtct tttgaaaaca ttaacttatc gccaacttta agatgatttg
     4861 tcttcgcaaa agcctgatga acaattataa ctcgctgatc tttcggcgtg atatgtcgcc
     4921 cttccttaag agaaatcatc cccgctgtgt atttaggatc tgtatcggag taactattac
     4981 catgaacttg taaaacttgt tctttatatt gttctttatc atttggtcgt gtaaccattt
     5041 tttccgaggt tagtccagca taagccatca aattattatt aacgcgctta acatttggat
     5101 tacttgctat gttaactatt gctttttcag ggatttctcc agatccgtaa ccatcaccaa
     5161 caaggtcagt actatccgat gaaatatgca aaattgatcc agtttgcctt gaaaatgtct
     5221 tcttagcagt aagaccagca ttttttaccg caataccgct aattaatgta gaagcaacta
     5281 gccataaaca tacgaataaa gtgatactct tacgcttttt acggataaca tataaaaagg
     5341 aacgtttaaa taaattcata ttaacctcct atgacattcg tgacaatatt tgctttggtt
     5401 gtagtttaag aatagaataa ctagataata caactgcaaa taagcaaatt aaagcaccct
     5461 gagcatagag tctaaagaca tccattactc caacagaaac agtgaggtta tcaattctat
     5521 caataattag actactattt tgtacaattt gtgaactcac accaccatta acttgtgata
     5581 aaaggccagc tgcaaatgtt tttccaagaa agacaccacc aataatagat agtgtgaagg
     5641 cagcacctgc tactaacagt acttctatca gataatgacc tatgatttca aacttagttt
     5701 ttccgatagc gagtaaaatt cccgtctctt taatgcggtt attaatccaa aatgttagca
     5761 tgacaactaa tattgccatc gccacgagac taacaataag aatgattcgt cccactagac
     5821 gatgcaatgt ctcgatatta cgataagaat ctccataaaa ctctttagct gtttgaacat
     5881 ctaatttccc gaaatcaaac ggccattgtt tgatgttttg gataactgta tccactaaac
     5941 gtttatcact taagcgcact gcaaccgtct gataaccttt ttgtttacta atcttcctaa
     6001 ccaatgtttc ctgagcaatc aatgtattat caatattggg attaaaagta tttttagact
     6061 tagcactatt cgcatcataa attcctgcaa aggtattata aaaagccctt gaaatacttc
     6121 aaggtgcttt ttatagatgc atatgtagga caacatgttg cgagttagtt atctggtaat
     6181 aattctttta agtaaaccaa ttctacttta aaagtattat caaaagcata accataaata
     6241 tagccatcta tcattgtttg ataggtaata tctatagatt tcccacattt ataggaaatt
     6301 tctttttgta accatgcctt ataaaataat aatttagaat ccatatcact actaactgat
     6361 tctcctaggt aggtttggta ggtttgttct gatctatctt tgtatttctc aatatcaacc
     6421 ccagttgaac cactgggagc ataggttaga atatgatagt caccagaatt agagtaacta
     6481 atttttccct ttggcaaacc aattaaataa ctactgacac tagaaaaaga ttcctgacaa
     6541 atctcattgg taaaacctaa cttttttaag cattggtaca caaaataact ccctgttaac
     6601 agtttatctt tgcttacctg tctatttgct tgttgatatc tcatcaaccg atcgccagat
     6661 aaattattct ccaatgtaga ctgcctattt ttataaatca taatatagtt aactttgtct
     6721 ttttctgata aaggattcaa tttctgcctc cgctttttgt aaagatgcct ctgcttcttc
     6781 tttaatccct cggcttagtc ttatcatatg actcttatag atatcatagc ctctttcaag
     6841 acagctaatt gctttacgta tctctcttac atcaggtttc tttaccaaat aagcgcactt
     6901 ttctttactg aacttcttaa tattccaaaa ttgttcgtta aagaaaggga tacctataaa
     6961 gggaatacct tgtgctgcca tcgcataaac tgttccttgc ccaccgtgta taatagcgaa
     7021 ataaatctct ttatctatgg ttttaaaatc aataaaatca actacctcaa tataacttcc
     7081 tggaatgttt atagcctcac cacagcttgt cagtaaataa tgataatgcc ttggtaatgt
     7141 ctttaaaaaa gattccaccc aatctcgttt aaatgaagta ccaaagctga gcagtacagt
     7201 cttattgtct tgcttaattt ggctgcattt tgaaaaacct acattatcta tcaaaatagg
     7261 tcctatcgtt ttataggacg aatctttatc aaattcataa aacaaagagt aatctgttac
     7321 caagtttaga tcaccagtga ataattctaa agcattatta aaaggtttgc caccatattt
     7381 tttaatatag ttgttccacg ttttaacatt tgttgaataa gtagtgatta ctcttttgat
     7441 aaaatccttt gtttgaggct tactagcttt acttatcttc ccattaggca atattccttc
     7501 tgagttttca acaatttcac tgatatgtgt tgcccctgaa ataatccaaa ttaatggtat
     7561 gctttctaaa cgacaacttg taataataga tagacggtaa cctgtcaata cagcatcaaa
     7621 tttttcttgg cgtaacaagt ctctttcaat agaacagatg cgttcaattt cttcttctga
     7681 taaaaaatta aagtctgttg aaagagaaaa gaaagcatca tatcgcttac tattataaac
     7741 agatttatct tctacacagt ggtgataacg ataagcatca ttattaaata aaaatggata
     7801 ataattgtct ccaaggatat gaatatcatg tccttgttca tataaataat tagccagttt
     7861 tgataatctt tggctctctc ctaaattcat catgaaagga gcaaataaaa atttactcat
     7921 cttatcacct tttttcaata atagcaaaag agataccacc aaagatcgaa taagatagga
     7981 ctaaataata gcccttttct atactttcat taacagtata gtccagttct tcaccagcac
     8041 cattagatga aaatccaaac tgaccaaaag caaggtttgg catattataa tactcagaca
     8101 agttcgctaa gaaatcataa tctgaactaa ctgccttctt ccgctcattc caaacgaaac
     8161 ctttgatatc ttttatggtt agtcctaagt ctgataataa attttgaagc gcagcatcaa
     8221 aaatagtcat cacatctgtg aatgttttat ggctatattt taattgttta ctacctaata
     8281 ttataggaga attctccaat gcttgacgag agaggacttg tgctgaacaa taatcagaac
     8341 caacaaacat ttgactatca tagtttaatt gttgccacca cataaaactc atgtctgtcc
     8401 actgattagc agaaacaaga atcacatagt ctagattatc gttacgcatc atttccttgg
     8461 catattgtat accatcaaga gctccactat ttgtcgaaat gacagataaa ggacctgtta
     8521 ttttaaaaat gatagaaagc ataccagctg ctgcattcat tactgtaaac gggaatcgtg
     8581 aagcagaaac atgtgcatat ccttctgttg tgatttgctt ttcaatacct tcaacaacct
     8641 caactggtcc agaaagtgtt gtaaatacaa ttcctacttt tgaagtatct tgttttttta
     8701 gattaatatt gctttctatt agtgcttgag ctgttgttac ggcaaccatt ttggaaaaat
     8761 catccatttt cctaaattgt gctgggttga cagttttggg tggtctagcc cctttaaagc
     8821 gtaatgcttc aaagtcgttg aaattactag caactttttc ataggttata gaaagtgatt
     8881 cattcttaga aatggaagca acagatgata agatagccat tttaagattt tctctagcag
     8941 gtaatgtttc tagaggtgaa tctaaagatg acaataagat accactatta tttccaccaa
     9001 aagcaaacga aaaatttaaa gcatttctta ttgggtattc tctcttttga tgatagacaa
     9061 aattttctgg aaaaccttct atcccaatct catttttagt tgctggtaca gtctgttcct
     9121 ctattgccgc taaacaatta atcaattcga taatacctgc agcccctaga gtatgacccg
     9181 tttgcccctt ggtactgctg atcaatgtcg ttgtcgggaa aaacttacca tacatatttt
     9241 tttccatttt atcattagct tgagtacctg taccgtgacc gttaatatag tcaatctcac
     9301 tgtagtcaat acctgcttga gtcactagct gctttgcaat ctgtgccgcc ccttcacctg
     9361 ttggcttagg tgctgttata tgataaccat ctgaagtaat aagaccaccg ataatttttc
     9421 catatttagc taaggactga tctttgacaa gaacaacaaa accagcgccc tcacccaaat
     9481 tgattccttt tccagaagaa tagggctgac atgccatttc tgtattaata gctcctagtg
     9541 atgtgaagcc tgctaaagaa atatcactta actcatcaca gccaccacaa atagctaaat
     9601 cacaatcgcc atcttgaagt aattgtgttc ctaatattac ggcattatta cttgcagaac
     9661 aggcggttga aataacatac gaagctccca caatatcatg ataagccatc aattcatcag
     9721 caatatggta aacagatgct ttttctaata aactagcatc tacttgacgc tctccttctt
     9781 caaattgata caaggcattt tgaccagcac tctttccccc aagtgaggtc cctaaacaca
     9841 cagcaatatt atgataagct tttaaattaa cacctgaaga agcaagagcc tcttcaaaag
     9901 cggtaaaagc aaatttaaaa ttacgtgtct catctttgta ttgctcagga acctctgggt
     9961 cactagttat gcttcctgta taagattcta aaatagagtc gtgattttta tataaatgtt
    10021 tagaaattcc ttcttttaag tcgaagagat gctgtttatg ctcgctataa ttctttccca
    10081 aagaagaaat aattcctatt ccactaacat atacgctcat tctttgttcc aactttctgg
    10141 gatacaataa gggcttgatt gtgttttcaa aatttggcca tcttctgtta caaaagtaac
    10201 tcctgaatga gcatatgttt ggttaacaat catcaaacct aggtaatttt ccgaagagtc
    10261 tttctcgtct gctatccaaa aaatctttcc aacaatttgg tcatcaaata gcactggtgt
    10321 tcctgaagcg agtttttcct tcgttgagaa tccaacactt ttaactgttg ctgatctgat
    10381 gtgctctaac aagctatcgc gacctctaaa ttcttccttt gtgaaatcta gattccaaga
    10441 ataacctatt tcatataaag aatattcttc ttgttgataa atatcagtaa tgggttgtcc
    10501 cacttcaaac cttaaatatc tatcaagttc atccccatac tctgctatac cttcacagac
    10561 atccgaaaca aaagtagcaa aaatagaaga tggtaggaaa aattgatagc caaattctcc
    10621 agaaaaacca aatctagcaa gataaccttt ctcaccttta taagtcatct ctatcaaagt
    10681 acgaaaatta agtgttgaaa tatcatattc ataaaatgac tgagcaattt ctcccgaata
    10741 tcttccttca atttgcagca ttttatactc gtcagaaata tctgttacgg tatagtcaaa
    10801 attgatgttg tctaaataag aatccaaagc tttatgacta gccaaccaat atttatcatc
    10861 aaatttgtag aaagtcacat catcataaac tgttccatct tcattgagta aaaaagtgta
    10921 gccacaagta tctgtatctg caaatacaat attcttagga atcacaaggt ctaaggcttc
    10981 ttcacattct tcaccagtaa tttcatataa atacccctca taaactttat aacctttatc
    11041 tgttctcaat gtattataaa tagtattcat ttttcttcac actttctata ttctgtaatg
    11101 ctcacatttg tagcataagg tgagttcctt ttaaacatgc accttatttt t
//
LOCUS       DATRTN010000058        11144 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.058, whole genome shotgun sequence.
ACCESSION   DATRTN010000058 DATRTN010000000
VERSION     DATRTN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11144
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.058"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..225
                     /locus_tag="VBE22_001426"
     CDS             <1..225
                     /locus_tag="VBE22_001426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202877.1"
                     /translation="LKRDGVLIEDFTEGVKIFEKPYKKSPEDNYLKSIEDYQKLLAYL
                     KRIANYSDSIIPFFFSQCYKLVLGLVKQWQ"
     gene            363..661
                     /locus_tag="VBE22_001427"
                     /pseudo
     CDS             363..661
                     /locus_tag="VBE22_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(797..867)
                     /locus_tag="VBE22_001428"
     tRNA            complement(797..867)
                     /locus_tag="VBE22_001428"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(833..835),aa:Thr,seq:ggt)
     gene            complement(938..1243)
                     /locus_tag="VBE22_001429"
     CDS             complement(938..1243)
                     /locus_tag="VBE22_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEO7202878.1"
                     /translation="MFPNLLSLISYHEQQIASRRLSHHLGLEQSIVLYAMAKEETISN
                     ERCPHPKLINVLEGKLEIILGDQKHILEKDSLISLSPQEIHHLRAIENSKFLQIELD"
     gene            complement(1245..1583)
                     /locus_tag="VBE22_001430"
     CDS             complement(1245..1583)
                     /locus_tag="VBE22_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO7202879.1"
                     /translation="MSYIEKIEANKVLDLKQIVPIEEEQMLSKTLVQRKDLGMTVFSL
                     DKGQEIGRHSSPGDAMVNILSGLAQITIDQDKFDVAAGECIVMPANIPHSLYAKEAFQ
                     MLLVVVKPEV"
     gene            complement(1604..2359)
                     /locus_tag="VBE22_001431"
     CDS             complement(1604..2359)
                     /locus_tag="VBE22_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7202880.1"
                     /translation="MSHKEIGHQFLARLGKKRLRPGGVQATNWLIKKGHFSADSRVLE
                     VACNQCTSSIDIAKAFSCHITALDLDSKVIEKAKENVKKAQLEEFIEVIQGNALKLPF
                     PDNSFDIVINEAMLTMLSNSAKEKAIKEYLRVLKPGGRLLTHDVSYQDEDTAKLIDQL
                     RHTINVNVAPLKIDDWQNLFLQNGFSSSEYQFGKMSLMSPLGMIKDEGLFNTIKIISR
                     GLKKENRSQFLKMKHFFTKTGKNLCYIAVASTK"
     gene            2577..4619
                     /locus_tag="VBE22_001432"
     CDS             2577..4619
                     /locus_tag="VBE22_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7202881.1"
                     /translation="MLLQQLAQSRLATLEQLATQFKTSTRTIRKDIDMINDCLIKAGA
                     DLIYFGNEGEVCSDKAKNHLNDIPKMISLTDYRLSREERLILESYFLLVSLTPITIKG
                     LSRKMYLSRNTVVNDLPSLRSFLTQNELILKSDKQGLIIEGSESQKRLLLMHHYISLS
                     DNDFLREYLFPSEDDPSLLISVVRKAEKQNQVVFRDYSFQYVQTYLNVLCSRSANNLM
                     IETEGMFHFSAFSLIISQSILEDLSQKVRFPLSLKEVEFLAFLIGNLTFVKKSSPIDD
                     SFKIQFLTRQFIKQISGDIAYDLTQDISLLENLSKHMETIFYNQPLQAPDSSLLTDII
                     ESNKSIVTAIKKHLSIFHILKSKVFEPNDIYYIAIHICGAVERQRLEIAHFKVLLACH
                     AGIGTSMLLKQRLKEHFNFDIIDVVTIHEAEEEQHRADFIITTVPLQKTNNETIVVSA
                     SLTETDIIRINTLMRKISKEGNVEENPETSQALFKDLLPILKKIGCEQKDLVMIRQVI
                     NRHCQKSMSPFEESMAHQVIQLSDLLTPNYIDLQGKASSWEESIRQSARLLREESLIE
                     ESYVEAMIANVLSYGPYILVAKGVAVAHAGIDDGVNGLGMSLYRLDKGVNFGNPELDP
                     IRYIICLCVTDYGKHVTAFNTLLNLFQDSSMREKLDRVDTAKDCYCLIKKYEEKEN"
     gene            4734..5183
                     /locus_tag="VBE22_001433"
     CDS             4734..5183
                     /locus_tag="VBE22_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7202882.1"
                     /translation="MIEKLIYNDLISLNHRYRDQTNLLEVLSQYLLQKGYVKTEFSKA
                     ILQREKDYPTGLQLENMAVAIPHTYSEYVLKPFIYINKLKEPISFIQMGTEDEIVMAR
                     YVIVLGISNPKDQAGLLAELMTLFSNPKIVQQLEMAQTKEALKNIFN"
     gene            5202..5480
                     /locus_tag="VBE22_001434"
     CDS             5202..5480
                     /locus_tag="VBE22_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7202883.1"
                     /translation="MKKLIVACGSGVATSQTVASKVDRLLKEAGISCDIEAVDMKSLE
                     QHLKTADAYISIVKAKKDYGVPVFNGVAFLTGMGQKQELEKIISFVKG"
     gene            5492..6829
                     /locus_tag="VBE22_001435"
     CDS             5492..6829
                     /locus_tag="VBE22_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO7202884.1"
                     /translation="MDVLQTVINYILDLGAAVFVPFLMLIIGLCMKMKFRDAFTSALI
                     LGIAFTGMGILVNYIMTSMGAAANDLTKHTGLSLPAVDIGWPGAASISWAWPYAFLVF
                     PLQLAINFVMLLLNQTKTLNVDLWNVWNKIFTAVIVTYFTNNVIFGFIAAAIIIILEL
                     KLGDVFAPEVERLTGVPGVTVPHFICLIAVILHPIDELLKKIPVLNKDFDADTLKEKI
                     GVFGENSVMGAIIGFVLGIASGNGIKFALTLAVQAATALTLFPMVSKLFSQALSPISE
                     AVSDFMREKFEDREVYIGLDWPILGGRNELWVAVIFTIPFLLIGSIILPGNIVLPFAG
                     IINLSFVVGALLLTNGNVIRMIIHGLISTPLFLYGATYFTPYMTRLAKETGTLKQKGL
                     ISWATFEGPDIRYLLATVFQGKVLSVILLIIWLALYVWLLKERKKYNTNLIVE"
     gene            6852..7682
                     /gene="rhaD"
                     /locus_tag="VBE22_001436"
     CDS             6852..7682
                     /gene="rhaD"
                     /locus_tag="VBE22_001436"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEO7202885.1"
                     /translation="MTYPDYINELLEVTYDMWQHGWDEYNGGNVSYRLTKEEIGTLRH
                     DLEDTLFTYNDSQEEVDVLSVPDNMQEEYLLITASGSHFRTLKNQPEIDTGIIRITST
                     GYQVIAGFNSGKKPTSEIFMHILAHSARVSQNPNHRVVIHNHATNIVIYSLLEEITSR
                     RLTIDLWSVLTESIVVFPDGIAVLPWEVPGTYQIGLDTAHELKKHRLVVWAKHGVLST
                     GVSYQDCFGLIETADKAANIALKLRSTSGKSIQDNNILTTDDLEKVCQKLQVKGRYLL
                     "
     gene            7817..8281
                     /locus_tag="VBE22_001437"
     CDS             7817..8281
                     /locus_tag="VBE22_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO7202886.1"
                     /translation="MLEDKVIVFQKSHLDSSEEALDYVSHLLLQSGAVKESYPNAIQE
                     REKAFPTGLMFSDYGVAIPHTDIEHVNYSQMAIVSLKNPVVFQQMGNGEDVEVNIIFM
                     LALKEAHQQVDLLSQLIELFQNKVLLKTLLALGNERSAIDQALGLLKAQGIE"
     gene            8294..9625
                     /locus_tag="VBE22_001438"
     CDS             8294..9625
                     /locus_tag="VBE22_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO7202887.1"
                     /translation="MLDILQKFVNLGAIVVLPILIFIFGILLGTKPRKAFISGVTVGI
                     GFVGLNMVVDLLGNSLGPAAQAMVKRFGLHLTTIDVGWPAASAISYGTLLGSLAIPIG
                     VGINLLLLFLGMTKTLMVDMWNFWHAAFVASLVYAVTHNFALGLYATVVYMVMIYLLG
                     DIIAPMIKTFYGFPNITFPHGTSAPGVIFAIPLNWLFDRIPGFNKIDAKPETIQKRFG
                     VFGDTTVMGLIIGIVMGLLANYNVTNTLQLGIKTAAVMVLMPRMVALLMEGLTPISES
                     ANTFVQKRFPGREVNIGMDSALSVGHPAVLSSSLILIPITILLSVILPGNTTIPFGDL
                     ATIPFAVCLMAAVFGGNIIRTVVGGTIYMAVIMYITSWVAPLVTLSAKAANFDLSGHS
                     RITALVEGGLWPTWLFVFAAKTLPWLILTLILIISLSVMYYLNKVKKEGEI"
     gene            9627..9911
                     /locus_tag="VBE22_001439"
     CDS             9627..9911
                     /locus_tag="VBE22_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO7202888.1"
                     /translation="MKKLLIMCGSGIATSTVVVGKVKTWLEENHYQDQVRIYQSKIAE
                     EINHIDDYDIVISTTVVPDNVQDKVIMGLPLLTGMGVDAVWSELEAQIKD"
     gene            complement(10196..11026)
                     /locus_tag="VBE22_001440"
     CDS             complement(10196..11026)
                     /locus_tag="VBE22_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO7202889.1"
                     /translation="MNHKELHLFNNLSYIDLYPIQFGKEACAPLHSFGPSLKEHYLFH
                     YIVSGRGTFYNTEEETAHKLEAGQGFLIPPHLVCSYEADKADPWTYIWIEFDGLKSDY
                     FLKQAGLDHKHLIFSQEKNSFDSPVYTEAINILASHNQRSAKVIAHLYLFIDALISQS
                     INHKVIPHNETKDFYIREAINFIERNFQFPITVEEIAKSCNLNRHYFCRLFREQTNSS
                     PQQFLIQYRLSKACALLKNRNLNLSDIAEQIGYSNQFNFSAAFKRQYGISPRQWRKTL
                     "
BASE COUNT         3550 a         1684 c         2129 g         3781 t
ORIGIN      
        1 ttaaaaaggg acggggtttt aattgaagat tttacagaag gagttaaaat ttttgaaaaa
       61 ccatataaga agtcgccaga agataattat ttaaaaagta ttgaagatta tcaaaaatta
      121 ttagcatatt taaaaagaat agctaattat tcagactcaa taatcccttt tttcttttca
      181 caatgttaca aactggtttt aggtttggtg aagcaatggc aataacttgg gatgatattg
      241 attttgaaag aaatagtatt tatacatatc gaagatattc ttcaataaaa aaggacttta
      301 caaagcctaa aacaaggact tcaataagga gagttccagt aagtcatgat ttaaaagagt
      361 tattgcttga actaaaattt caacaagaaa gaatgctgaa agctttgcat ataaaaaata
      421 cagatcaatt gattttttat gactataggt ttggtatgat atcaaataat gctgttaaca
      481 aattcttggc aagctcattg gaaaattaga aatagaatct aagatgtcat caacaggtgc
      541 tagacatact tatagaagtt atttgttggc taacggagta gatatttggg cagttgcaaa
      601 attaatgggg cataaagata tcaaacaatt aatagaaaca tatggtcatt tgcttctgta
      661 agtggaagaa aaggaaaatg atcagctttt aaaaattctt aataataaag gtagttttta
      721 aaatgactat caagaacaaa ttaagaacaa aaataaaaga aaccctatta taataaggtt
      781 tcactcatat attttctgcc accaccgaga attgaactcg gaactgagca ttaccattgc
      841 tctattatac catttaacta tagcggctac ttttctatga tactataaga ttttgacgaa
      901 atcaagtatt tctttactta gaaaaatagt cgttttttta gtctaattct atttggagaa
      961 atttactatt ttcaatagct cttaaatggt gtatttcttg tggggagagg ctaataaggc
     1021 tatctttttc aaggatatgt ttctgatcac caaggataat ttcaagtttt ccttccaaaa
     1081 catttattaa ttttggatga ggacatcgtt cattgctgat agtctcttct ttagccatag
     1141 cgtacaaaac gatactttgt tccagaccta ggtgatgact taatcgacga ctagcgattt
     1201 gttgctcgtg ataagaaata agtgatagta aatttggaaa catcttacac ctcaggcttc
     1261 acaacgacaa gaagcatttg aaaggcttct ttagcataga gactgtgagg aatgttagca
     1321 ggcattacaa tacattcacc agctgctacg tcaaatttgt cttgatcaat cgtgatctgc
     1381 gcaagtccac taaggatatt taccatagcg tctccgggag atgagtggcg tccaatttct
     1441 tgccccttgt ctaatgaaaa gaccgtcatt cctaaatctt tccgttgaac taaagtttta
     1501 ctaagcattt gctcttcttc aataggtacg atttgtttta aatcaagaac tttattggct
     1561 tcaatttttt cgatataaga cataataact cctttttaat agtctatttt gtactagcga
     1621 cagcaatata gcagagattt ttaccagttt tagtaaagaa atgtttcatt tttaaaaatt
     1681 gggaacggtt ttcctttttc agtcctcggc tgatgatttt aatggtattg aataaacctt
     1741 catctttaat cattcctaat ggggacataa gactcatttt tccaaattga tattctgaac
     1801 ttgaaaaacc attttgtaag aataaatttt gccaatcatc tatttttaga ggagcaacgt
     1861 taacatttat agtatgtcta agttgatcga tcagcttggc agtgtcttca tcttgataag
     1921 aaacatcatg tgtgagtaaa cgtccaccag gttttaggac acgtaagtac tcttttatgg
     1981 ctttctcctt agctgaatta ctaagcatcg ttagcattgc ttcgttgata acgatatcaa
     2041 aactattatc aggaaagggc agtttgagtg catttccctg aataacttca ataaattctt
     2101 ctaattgagc ctttttgaca ttttctttgg ctttttctat tactttacta tctagatcaa
     2161 gcgctgtaat gtgacaggaa aatgccttag cgatatcgat ggaactggta cattgattac
     2221 aagctacttc tagaacacga ctgtcagcac taaagtgtcc ttttttgata agccaattcg
     2281 tcgcttggac acctcctggt cttaagcgtt tttttcctag tcgtgctaag aattgatgtc
     2341 caatttcttt atgtgacaat aagatcacct ctttctttta atagtaatat cattttttct
     2401 taactataac acatagatgc taagataaca aggagtaata gtgattgaat ggtataaatg
     2461 atgaatttta gaaaacgttt tctttgtaat taggaatgtg gtatcatgaa aatgagtggc
     2521 gctacgctag acagcatttc tttaagcacg aggttactat gaataagaga acgtttttgt
     2581 tattacaaca actagcacaa tcaaggctag caactttaga gcaattagca actcaattta
     2641 aaacttcaac acgtacgatt cgaaaagata ttgatatgat taacgattgt ttgattaaag
     2701 caggagctga tttaatttat tttggtaatg agggtgaagt ttgctctgac aaagccaaaa
     2761 atcatttgaa cgatattcct aaaatgattt ctctcacaga ctataggtta tctagggaag
     2821 aaaggttaat tttagaatct tattttctat tagttagctt gactcctata accatcaagg
     2881 gcttaagtcg aaaaatgtat ttgagtcgta atactgtggt taatgattta ccttcattac
     2941 gctctttttt gacgcaaaac gaattaattt tgaaatcaga taaacaaggt ttaatcattg
     3001 aaggttctga atcgcagaag cgtttattgt tgatgcatca ttatatttca ctaagtgata
     3061 acgattttct cagagaatat ctatttccta gtgaagatga cccatcgtta ttaatttcag
     3121 tagttcgtaa agctgagaaa cagaatcagg ttgtttttcg cgattactca tttcaatatg
     3181 ttcaaactta tttgaatgtt ttatgttctc gttctgctaa caacttgatg attgaaacgg
     3241 aaggaatgtt tcatttttct gcattttcct tgattattag tcaatcaata ttagaggatt
     3301 tatctcaaaa agttagattt cccttatcgc ttaaggaggt tgagttttta gcttttctaa
     3361 ttggaaattt gacttttgtg aagaaatctt ctccgattga tgatagtttt aaaatccaat
     3421 ttttaactag acaatttatc aagcaaatat caggagatat tgcatatgat ttgactcagg
     3481 acattagttt gctagaaaat ttgtctaagc atatggagac gattttttat aaccaacctt
     3541 tacaagctcc tgattcgtca ttgctaaccg atatcattga atccaataaa agcattgtca
     3601 cagccattaa aaagcatttg tctatcttcc atattctcaa atctaaggta tttgaaccca
     3661 atgatatcta ttacattgct attcatattt gtggtgctgt ggagcgtcag cgtttggaaa
     3721 tagcacattt taaagtttta ctggcttgtc atgcgggtat agggacatca atgttattga
     3781 aacaacgttt aaaagagcac tttaactttg atattataga tgttgttacc attcatgaag
     3841 cagaggaaga gcagcatcga gctgatttta ttataacgac ggttccactc caaaagacaa
     3901 ataatgaaac gattgttgtc tctgcttctt taacagaaac agatattatc agaatcaata
     3961 cattaatgag aaaaatcagt aaggaaggca acgtagaaga aaatccggaa accagtcagg
     4021 ctttatttaa agatttgttg ccaatactaa aaaaaatagg ttgtgagcaa aaagatttgg
     4081 ttatgattcg gcaagttata aatcgtcact gccagaaatc tatgagccca tttgaggagt
     4141 caatggctca tcaggttatt caattatcag atttattaac acctaattat attgacttgc
     4201 aagggaaagc gtcctcgtgg gaggagtcta taaggcagtc ggctaggctc ctacgggaag
     4261 agtcattaat tgaggaatca tatgtagaag ctatgattgc aaatgtactt tcttatggtc
     4321 catatatatt ggttgcaaag ggagtagctg ttgctcatgc aggcattgat gacggtgtta
     4381 atggtttggg aatgtcgctt tatcgcttag ataagggagt aaattttggc aatccagaat
     4441 tggatcccat ccgttatatc atttgtttat gtgtaacaga ttacggtaag catgtgactg
     4501 cttttaatac tctgcttaat ttatttcagg atagtagtat gagagaaaag ttggataggg
     4561 ttgatactgc taaagattgt tattgtctga ttaaaaaata cgaagaaaag gaaaattagt
     4621 aagaccaaaa tacttcataa aaactgaagt attttggtct ttttgtgtga acattttaaa
     4681 tttataataa agacagtaaa agctatcttg cagaaggagg agaactattg tctatgattg
     4741 aaaagttaat ctacaatgat ttaatttctt taaatcaccg ttatcgagat caaacaaatt
     4801 tactagaagt tttgtctcaa tacttattgc aaaaaggcta tgttaaaaca gagttttcaa
     4861 aagctatttt acagcgagaa aaagattatc caacaggact acagttagaa aatatggctg
     4921 ttgctattcc tcacacctat tcagagtatg tccttaagcc ctttatttat atcaataaat
     4981 taaaagaacc tattagtttc atacaaatgg gaacagagga cgaaatagtg atggcacgtt
     5041 acgtcatcgt tttagggatt tcaaatccta aagatcaagc aggtttacta gctgaattaa
     5101 tgacactctt ttctaatcct aaaatagttc aacaactcga aatggcacaa accaaagaag
     5161 ccttaaaaaa tatttttaac taattgatgg aggtatttat tatgaaaaaa ttaattgtag
     5221 cttgtggttc gggagttgcg acctcacaaa ctgttgcatc taaagtagac cgtttgttaa
     5281 aagaagcagg aattagttgc gacattgagg cagtagatat gaaatcttta gagcaacatt
     5341 taaaaacagc tgatgcttat atttcgattg ttaaagccaa aaaagattat ggtgttcctg
     5401 tctttaacgg cgttgctttc ttaacaggaa tgggtcaaaa gcaagaattg gaaaaaatta
     5461 tttcattcgt taaaggttga ggagtagcgt tatggatgtt ctacaaactg ttattaatta
     5521 tattttagat ttaggtgctg ctgtttttgt tcctttttta atgcttatta tcggtttatg
     5581 catgaaaatg aaattccgtg atgctttcac atctgcactt attctcggaa tagcttttac
     5641 agggatggga atcctggtta attacattat gactagtatg ggtgccgcag cgaatgattt
     5701 aacgaaacat accggtttaa gtttaccagc agttgatatt ggatggcctg gagcagcctc
     5761 tatcagttgg gcttggcctt atgcttttct tgttttccca ttgcagctgg ctattaattt
     5821 tgtcatgtta ctcttgaatc agaccaaaac acttaatgtg gatttatgga acgtttggaa
     5881 taagattttt actgctgtaa ttgtaactta ttttaccaat aatgttatct ttggttttat
     5941 tgcagcagct attatcatta ttttagaatt aaaattagga gatgtctttg ctccagaagt
     6001 tgagcgctta acaggtgttc caggtgtcac agtcccacat tttatttgtt taattgctgt
     6061 aattttgcat cctattgatg aactattgaa aaaaattcct gttttaaata aagattttga
     6121 tgcagataca ctaaaggaaa aaataggagt gtttggagaa aattcagtta tgggagcgat
     6181 tatagggttt gtcttaggga ttgcttctgg taatgggata aaatttgcgt tgaccttagc
     6241 tgttcaagct gcaacagcct tgactttatt tcctatggtt tcaaaattat tttctcaagc
     6301 gctttcccca atttctgagg cagtgtcaga ttttatgaga gaaaaatttg aagatcggga
     6361 agtttatatt ggtttggatt ggccaatact aggtgggcga aatgaacttt gggtggctgt
     6421 tatttttacc attccattct tattaatagg ttcaattatt ctaccaggga atattgtttt
     6481 accatttgca gggattatca atctttcatt tgttgtagga gctctattgc tcacaaatgg
     6541 caatgtaatc cgaatgatta tccatggact tatttcgact cctttattct tatatggagc
     6601 aacttatttc acaccatata tgactagact tgctaaggaa actggtactc taaagcaaaa
     6661 gggtctaatt tcttgggcga cttttgaggg cccagacatt cgataccttt tggctactgt
     6721 ttttcaaggt aaagtattat ctgttatttt acttatcatt tggttggcat tgtatgtttg
     6781 gctattgaaa gaacgcaaaa aatataacac gaatttgatt gtggaataaa agagaagaca
     6841 gagggaaaaa tatgacatat ccagactata ttaatgaact tttggaagtc acgtatgata
     6901 tgtggcaaca tggttgggac gaatataatg gcggtaatgt gagttaccga ttaaccaaag
     6961 aagaaattgg tactttaaga catgatttag aagatacctt atttacttat aatgacagtc
     7021 aagaagaagt agatgttcta tctgtaccag ataatatgca agaggagtac cttcttatca
     7081 ctgcttctgg tagtcatttt agaacattga aaaatcaacc agagattgat acaggcatca
     7141 taaggataac ttctactggc tatcaagtta tagcaggctt taatagtgga aagaaaccaa
     7201 caagcgaaat ttttatgcat atactagcgc attctgcgag ggtaagtcaa aaccctaacc
     7261 accgtgttgt tattcataat cacgctacta atattgtcat ttattcccta ttagaagaaa
     7321 taactagtcg acgtttaacg atagatcttt ggtcagttct aacagaatca atagttgttt
     7381 ttcctgatgg catagcagtt ttaccatggg aagttccagg aacttatcaa atagggttag
     7441 atacagcaca cgaattaaag aaacatcggt tagtcgtttg ggcaaagcac ggtgtccttt
     7501 caacaggagt tagttatcaa gattgttttg ggttgattga aacagcagac aaggcagcta
     7561 atattgcact taaacttaga agtaccagcg gtaagtcaat tcaagataat aatatattga
     7621 ccacagatga tttggaaaaa gtatgccaaa agttgcaagt aaaagggcgt tacttattat
     7681 aattagctca tgaatgatgg ataatagtaa caggagacca aaatgcttca ctttgagtga
     7741 agtattttgg tctttttatg aaatcgattc catattataa taaaaccaag aaaacctaag
     7801 taaaggagag gcgctgatgc tcgaagataa agtcattgta tttcaaaaat cacatttaga
     7861 ttcttcagaa gaagcattgg attatgtatc ccatttattg ctacaatctg gtgctgttaa
     7921 agaaagttac cccaacgcta ttcaggagcg tgaaaaagca tttcccaccg gtctaatgtt
     7981 ctcagactat ggtgttgcga ttcctcatac tgatattgag catgttaatt attcacagat
     8041 ggcaatagtt agtcttaaaa atcctgtagt ttttcagcaa atgggtaatg gagaagatgt
     8101 tgaagttaat attatcttta tgttagcttt aaaggaggct catcaacagg ttgatttatt
     8161 gtcgcagtta attgaacttt ttcaaaataa agtattactt aagacattat tagctctagg
     8221 taatgaaaga agtgctattg accaggctct tggtctttta aaagctcaag gaattgagta
     8281 ggaggtcttt ttaatgttgg atattttgca gaaattcgtt aatttaggag caatagtcgt
     8341 tttgccaata ctgatattta tctttggtat tttactggga acgaaaccta gaaaagcatt
     8401 tatttctggg gtgactgttg gtattggttt tgtaggatta aatatggttg ttgatttact
     8461 aggtaatagt ttaggtcccg cagcacaagc tatggttaaa cgatttggcc tacatttaac
     8521 aacgattgat gtcggatggc cagcagcctc agcaatttct tatggaaccc ttttaggaag
     8581 cttagccatt ccgattggtg ttggtattaa ccttttactc ctatttttag gaatgactaa
     8641 aacattaatg gtagatatgt ggaatttttg gcatgcagct tttgtcgcct cgcttgttta
     8701 cgcagtaact cataattttg ctctaggttt atatgccaca gttgtttata tggttatgat
     8761 ttatcttcta ggtgatatta ttgcgcctat gatcaaaaca ttttatggct tccctaacat
     8821 tacttttccg catggaacct cggctccagg ggtaatattt gctattcctt tgaactggtt
     8881 gtttgaccgc atcccaggtt tcaataaaat agatgccaaa cctgagacta ttcagaagcg
     8941 gtttggagtt tttggtgata caacagtaat ggggttaatc attggtattg ttatgggact
     9001 tctggctaat tataatgtca ctaatactct tcagctaggt attaagacag cagcagtcat
     9061 ggttttaatg ccacgaatgg ttgccctttt aatggaagga ttaacgccta tttcagagtc
     9121 agcaaatact tttgttcaaa aacgttttcc tggtcgtgag gtcaatattg gaatggattc
     9181 agcgttatca gttggacatc ctgctgtttt atctagttct ttaattttga ttccgataac
     9241 tattttatta tcagtcattt tacctggtaa tacgaccatt ccttttggcg atttagcaac
     9301 catcccattt gctgtttgtt tgatggcagc agtatttggt ggaaatatta tacgtacggt
     9361 cgtaggaggt actatctaca tggcagtgat tatgtatatc acttcatggg ttgctccttt
     9421 agtgacatta tctgcaaaag cagctaattt tgatcttagt ggtcattcac gaatcacagc
     9481 tctagtagaa ggtggtttat ggccaacgtg gttatttgta tttgcagcca agacactacc
     9541 gtggctgata ctaactctta ttttgatcat tagtttatct gttatgtatt atctcaataa
     9601 ggttaaaaaa gaaggagaaa tttaagatga aaaaattatt gattatgtgt ggctcaggga
     9661 ttgcaacgtc aacagttgtg gttggaaaag taaaaacatg gctcgaagaa aatcactatc
     9721 aggatcaagt gcgtatttac caatcaaaaa tcgctgaaga aatcaaccat attgatgatt
     9781 atgatatcgt tatcagtacg acagttgttc cagataatgt ccaagataaa gttattatgg
     9841 gtttaccgct attaactggt atgggggtcg atgctgtttg gtcagaactt gaagcacaaa
     9901 ttaaggatta ataaaagaag agtaatgttt tttgtaagta aggctgatac atgattttag
     9961 ccttactttt ttcttttgag ataacaattg catcaattag ttaaaagagg ccatgtctga
    10021 gggaaataag aagactcttt tactggcaac ttggttgtgc gatgcaaaag agtcgattgt
    10081 tattaattac tttgattatt ctattttttg ataggttcct tgtttattaa ccattataat
    10141 atggtgatat tatagggtta gtgtagtaaa tcttagctga tatttaacgt ttagtctaaa
    10201 gagttttacg ccactgtcta ggagatattc catactgacg tttgaaagca gcagagaaat
    10261 taaattggtt agagtagccg atctgttcgg ctatatcact gaggttcaga ttacggtttt
    10321 ttagcaaagc gcatgcctta ctcaagcgat actgaattaa aaattgttga ggcgagctat
    10381 tggtttgttc tcggaagaga cgacaaaagt aatgcctatt taaattacag gatttggcta
    10441 tttcctcaac agtaatagga aattgaaaat tacgttcaat gaagttaatg gcttcacgga
    10501 tatagaaatc ctttgtttca ttatgaggaa taactttatg gttaatagac tgactaatta
    10561 gggcatctat aaaaagatag agatgcgcaa tgacttttgc acttcgctgg ttatgacttg
    10621 ctaagatatt aatagcttct gtgtagactg gactatcaaa agaattcttt tcttgcgaga
    10681 agatcaagtg tttatggtct aaaccagctt gctttagaaa ataatcactt tttagaccat
    10741 caaattcaat ccagatatag gtccaggggt cagccttgtc agcctcgtaa ctacaaacta
    10801 agtgaggagg aatgagaaaa ccttgccccg cctccaattt gtgagcggtt tcttcttcgg
    10861 tattataaaa ggtacctcga cctgatacga tatagtggaa aagataatgc tcttttaatg
    10921 agggtccaaa agaatggaga ggagcacatg cctctttccc aaattgaata ggatataaat
    10981 caatataaga gagattattg aataaatgga gctctttgtg attcattatt aatttcctca
    11041 aagtttaata caaattaaag cgtttactta agcgtattat aacatagttt atatgagaat
    11101 gtgattattt atatgattaa gtgctaatat gaccataaat tcat
//
LOCUS       DATRTN010000059        11079 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.059, whole genome shotgun sequence.
ACCESSION   DATRTN010000059 DATRTN010000000
VERSION     DATRTN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11079
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.059"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            58..1023
                     /locus_tag="VBE22_001441"
     CDS             58..1023
                     /locus_tag="VBE22_001441"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEO7202890.1"
                     /translation="MTIKNLTVAGSGVLGSQIAFQAAYKGMSVTIYDINDEALNKGKE
                     RIKKLAKVYQSEIETAKEAYSDKAKSIKYNKNLLPSLDHIFLSKVADSLDLIADLPNQ
                     ITFSKNLDQAVSDADLVIEAVPETVSIKEDFYKQLAKVAPSKTIFATNSSTLVPSQFA
                     DITGRPDKFLAMHFANNIWQNNIVEIMGHKGTDDEVVKEALAFSKDIGMVPLHIHKEQ
                     PGYILNSILVPFLESALALYYNKVSDSETIDKTWKLGTGAPMGPLEILDIIGIDTAYN
                     IMKNYSDTNSDPNSLHAHLAKMLKEEFIDKGRTGKAAGHGFYDYD"
     gene            complement(1070..1618)
                     /locus_tag="VBE22_001442"
     CDS             complement(1070..1618)
                     /locus_tag="VBE22_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO7202891.1"
                     /translation="MTKALISIDYTYDFVADDGKLTAGKPAQSIASAIADVTEKAYRS
                     GDYIFFAIDNHDIGDVFHPETNLFPEHNIKGTSGRNLYGPLGTLYETIKEDSRVFWID
                     KRHYSAFSGTDLDIRLRERRIDTLILTGVLTDICVLHTAIDAYNLGYKIEVPAAAVAS
                     LNDSNHQWALNHFKTVLGATIL"
     gene            complement(1685..2470)
                     /gene="codY"
                     /locus_tag="VBE22_001443"
     CDS             complement(1685..2470)
                     /gene="codY"
                     /locus_tag="VBE22_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEO7202892.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLDAELPYNTMAAQLADIIDCNAC
                     IINGGGNLLGYAMKYKTNTDRVEEFFETKQFPDYYVKSASRVYDTEANLSVDNDLSIF
                     PVETKENFQDGITTIAPIYGGGMRLGTFIIWRNDKEFSDDDLILVEIASTVVGIQLLN
                     LQTENLEENIRKQTAVTMAINTLSYSEMKAVAAILGELDGLEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFDKLKEYN"
     gene            complement(2596..3807)
                     /locus_tag="VBE22_001444"
     CDS             complement(2596..3807)
                     /locus_tag="VBE22_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7202893.1"
                     /translation="MKIFDKSMKLEHVAYDIRGPVLEEADRMRANGEKILRLNTGNPA
                     AFGFEAPDEVIRDLITNARESEGYSDSKGIFSARKAVMQYYQLQNIHVDMDDIYIVNG
                     VSEGISMSMQALLDNDDEVLVPMPDYPLWTACVSLAGGNAVHYICDEEANWYPDIDDI
                     KSKITSKTKAIVLINPNNPTGAVYPREILQEIVDIARQNDLIIFSDEVYDRLVMDGME
                     HIPIASIAEDIFTVTLSGLSKSHRICGFRVGWMVLSGPRQHVKGYIEGLNMLANMRLC
                     SNVLAQQVIQTSLGGQQSIDSMLLPGGRIYEQRNYIHKAINEIPGLSAVKPNAGLYLF
                     PKIDTDMYRIDNDEEFVLNFLKQEKVLLTHGRGFNMNTADHFRIVYLPRVDELTELQE
                     KMARFLSQYKR"
     gene            4075..4527
                     /locus_tag="VBE22_001445"
     CDS             4075..4527
                     /locus_tag="VBE22_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO7202894.1"
                     /translation="MSQKYERILVAIDGSTESELAFEKAVNVALRNDSELILTHVIDT
                     RALQSFATFDTYIYEKLEKEAKDVLEEYEKQAREKGADKVRQVIEFGNPKTLLAHDIP
                     EKEKVDLIMVGATGLNTFERFLIGSSSEYILRHAKVDLLIVRDPNKTM"
     gene            complement(4583..5965)
                     /locus_tag="VBE22_001446"
     CDS             complement(4583..5965)
                     /locus_tag="VBE22_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO7202895.1"
                     /translation="MAIKAVFFDIDGTLLNDRKNVQKSTIKAIRNLKDQGILVGLATG
                     RGPSFVQPFLENLGLDFAVTYNGQYIYSRSEIIYTNQLSKTTVYRLIRYAGARRREIS
                     LGTASGLLGSGIIGLGTSRLGQIVSSLVPRKWAKAIERSFKHFIRRIKPQNIDSLMII
                     LREPIYQVVLVATEGESERIQKQFPRVKLTRSSPYSMDVISEEQSKVKGIERVGQRYG
                     FDLSEVIAFGDSDNDIEMLSQVGIGVAMGNASQQVKENARYTTADNNDDGISKALAHY
                     GLIQFEIEKTFSSRDENFNKVKSFHLLMDGETIETPRLYDSKEAGFRSDFKVEEIVEF
                     LYAASQGNQKVFDQSIRNLHLAIDKARDKVISKDHPETPLVGEVDALTDLLYLTYGSF
                     VLMGVDPKPLFDTVHEANMGKIFPDGKAHFDPVTHKILKPDDWEEHFAPEPSIRRELD
                     SQIQKSLNRK"
     gene            6034..6996
                     /locus_tag="VBE22_001447"
     CDS             6034..6996
                     /locus_tag="VBE22_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEO7202896.1"
                     /translation="MKKILVLHTGGTISMNANEKGQVMSSADNPMKYVDLSLDDLDLT
                     VVDFLNLPSPQITPHHMLDIYHYLKQHASNFDGVVITHGTDTLEETAYFLDTMILPKI
                     PIIITGAMRSTNELGSDGVYNYLSALRVANSTKAADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGIIMKQDLLFFKATEERVRFDLDKITGTVPIVKAYAGMGDSGII
                     SFLNSQNISGLVIEALGAGNMPPKAAQEIEELIEQGVPVVLVSRCFNGIAEPVYGYEG
                     GGARLQESGVMFVKELNAPKARLKLLIALNAGLTGQNLKDYIEG"
     gene            complement(7031..7909)
                     /gene="aroE"
                     /locus_tag="VBE22_001448"
     CDS             complement(7031..7909)
                     /gene="aroE"
                     /locus_tag="VBE22_001448"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEO7202897.1"
                     /translation="MNSLNGETLLTGLIANPARHSLSPLMWNTSFQEKNMNYAYLTFE
                     VEEGKLTEAVRGVRALGIRGVNVSMPFKQSVIPLLDDLSPQAKLVGAVNTIVNQGGTG
                     RLVGHMTDGIGCFKALAAQGFSAKNKIVTIAGIGGSGKAVAVQAAMEGVAEIRLFNRN
                     SSNYDKVIDLSDKIKKQFQIKVVVDYLENKTAFKDAIRTSHFYIDATSLGMRPLDNYS
                     LINDPEILTPNLVVVDLVYKPKETALLRFVRQNGVKHAYNGLGMLIYQGAEAFQLITN
                     QEMPISSVERVLQTEN"
     gene            complement(8007..8921)
                     /locus_tag="VBE22_001449"
     CDS             complement(8007..8921)
                     /locus_tag="VBE22_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO7202898.1"
                     /translation="MLQNIGQTGIQATRIALGCMRMSDLEGKQAEEVVGTALDLGINF
                     FDHADIYGGGLSELRFRDAIKHLNVNRDKMIIQSKCGIREGYFDFSKEYILSSVDGIL
                     ERLGTEYLDFLILHRPDVLVEPEEVAEAFTKLRAEGKVKHFGVSNQNRFQMELLQSYL
                     DEPLAVNQLQLSPAHTPMFDAGLNVNMLNKASIEHDDGIVDYCRLKRVTIQAWSPFQI
                     DLSRGLFVNHPDYKELNETIAKLAKNYNVSSEAIVIAWILRHPAQIQAIVGSMNPSRL
                     KAIDKANDIALTREEWYDIYRSAGNILP"
     gene            complement(9212..>11079)
                     /gene="recG"
                     /locus_tag="VBE22_001450"
     CDS             complement(9212..>11079)
                     /gene="recG"
                     /locus_tag="VBE22_001450"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEO7202899.1"
                     /translation="LVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSF
                     FNQPYLADKIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLI
                     KAIKSAFEISAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFE
                     ELFYFQMNLQVLKSENKSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDEILSDM
                     SSGAHMNRLLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFP
                     DLSIAILTSGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRF
                     GVKQRRIFREKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIITRWVK
                     HEQLGTVLEWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVAL
                     VHGRMKNDEKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQ
                     LRGRVGRGYKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTR
                     QSGIPEFQVADIVEDYPILEEARRVASDIVKDNNWKENTEWALILDNLRQHSDFD"
BASE COUNT         3586 a         2184 c         1764 g         3545 t
ORIGIN      
        1 tcttttgtta aactttcttc gaaaacgttt tcgaatataa gtatcggagg ttgtaatatg
       61 acaattaaaa atctcactgt tgcaggaagt ggcgttctag gtagtcaaat cgcctttcaa
      121 gcagcttaca aaggaatgtc tgtcaccatc tatgacatta acgatgaagc tttaaataag
      181 ggaaaagaac gtatcaaaaa attagctaaa gtctatcaat cagaaattga aacagcaaaa
      241 gaagcatata gtgataaagc taaaagtatc aagtataata aaaacttatt gcctagctta
      301 gaccatattt ttctttcaaa agttgccgat tcattagact tgattgctga tttaccaaac
      361 caaattactt tttcaaaaaa cttagaccaa gctgtaagtg acgccgattt agtaatcgaa
      421 gctgtcccag aaacagttag tataaaagaa gatttttaca aacaattagc taaagtggca
      481 ccttcaaaga caatctttgc aacaaattct tcaacacttg ttccaagtca gtttgctgat
      541 ataacaggac ggcctgataa atttttagca atgcatttcg ctaacaatat ttggcaaaat
      601 aatattgttg aaattatggg acataaggga actgacgacg aggttgtcaa agaagctttg
      661 gcattctcaa aagatattgg tatggtacct ttacatattc ataaagaaca acctggctat
      721 attcttaatt caatactagt tccattctta gagagtgcat tagcattgta ctataataag
      781 gtttctgatt ctgaaactat tgataaaacc tggaagttag gaacaggagc accgatggga
      841 cctttagaaa ttttagatat tattggcatc gataccgctt ataatatcat gaagaattat
      901 tcagatacca acagcgatcc taatagtctt catgctcacc ttgcaaaaat gcttaaagaa
      961 gaattcattg ataaaggacg taccggtaaa gctgctggac acggctttta tgattacgac
     1021 taaactataa aagaggttag ataaaaatct aacctctttt ataattcaac taaagaatag
     1081 ttgcaccaag aacagtttta aaatgattta aagcccattg atgattacta tcatttaagc
     1141 tagctacagc tgctgctggg acctcaattt tataacctaa gttataagca tcaattgctg
     1201 tgtgtaatac acaaatatct gtcaatactc ctgtcaatat caaagtatct attcgtcttt
     1261 cacgtaggcg aatatctaaa tcagtgccag aaaaggcaga ataatggcgc ttatctatcc
     1321 aaaaaacacg actatcttcc ttaatagttt catatagagt tcccaaagga ccatataggt
     1381 ttctaccaga agtcccttta atattatgct caggaaaaag attggtctct ggatgaaaaa
     1441 catctccaat atcatgatta tcaatcgcaa aaaaaatata atcccctgac cggtatgctt
     1501 tctctgtaac gtcagctata gctgaagcga tagattgcgc tggtttacct gccgttaatt
     1561 tcccatcatc tgccacaaaa tcatatgtgt agtctattga gatgagtgct tttgtcattt
     1621 ttcttcttat ctcctcttga tttcaaaaaa ggaactgaaa gctactccca gtcccatgct
     1681 atgattaatt atattctttt aatttgtcaa aaataccttc gttaataact ttgaggtatg
     1741 ttcccttcat accgagtgag cggctctcaa tgattcccgc agactccaat ttacgaagag
     1801 catttacaat aacggaacgt gtaattccaa tacggtctgc aataacagat gccgttaggc
     1861 gtccttcaag tccatctaat tctcctaaga tagcagctac agctttcatt tctgaataag
     1921 atagagtgtt gatagccatt gtcaccgctg tttgtttacg aatattttct tctagatttt
     1981 ctgtttgaag gttaagtaat tgaataccaa caacggtgct tgcaatttca actaaaatca
     2041 aatcatcgtc gctaaattct ttatcattac gccagataat aaatgttccc aagcgcattc
     2101 caccaccata aattggagcg attgttgtaa taccatcttg gaagttttcc tttgtctcaa
     2161 ctgggaaaat tgacaaatca ttatctactg ataagttagc ttctgtatca taaacacgac
     2221 tagcagattt aacatagtaa tctgggaatt gttttgtttc aaaaaattct tccacacggt
     2281 cagtgtttgt tttatatttc atggcatagc caagtaagtt accaccacca ttaataatac
     2341 atgcgttaca gtcaataata tcagctagtt gagccgccat tgtattatat ggtaattcgg
     2401 catctaagct atctacggaa cgctgtaaga tggatgtaat ttttctagtt ttttctaata
     2461 aattcggcat ttccttcatc ctttgtatgt tttgcgttac aattatatca aagcatttta
     2521 gcttttgcaa atcgatatca gtacatttta agaacttcta ttattaattt aatgtttatc
     2581 taataaaaat tcattctagc gcttgtattg gcttaaaaag cgagccattt tttcttgtag
     2641 ttcagtcaat tcatcaacac gtggtaagta aacaattcta aaatggtcag cggtattcat
     2701 attaaatcct ctaccgtgtg ttagtaatac tttttcttgt tttaagaaat taagcacaaa
     2761 ctcttcatca ttatcaatac ggtacatatc tgtatcaatt ttagggaaaa gatacagtcc
     2821 agcatttggt tttacagcag ataaaccagg tatttcatta attgctttat ggatataatt
     2881 acgttgctcg tagatacgac ctcctggcaa caacatacta tcaatagatt gctgtccgcc
     2941 taacgatgtt tgaataacct gttgagcaag aacatttgaa cacagcctca tgttagctaa
     3001 catattcaaa ccttcaatat aacctttaac atgttgtcgc ggaccagaca ataccatcca
     3061 accaacgcgg aaaccacaga tgcgatgtga tttagataag ccagataaag taactgtaaa
     3121 aatatcctct gcaatagaag caattgggat atgctccatt ccatccataa ctaaacggtc
     3181 ataaacctcg tctgaaaaaa taatcaagtc attttgtcta gcaatatcca caatctcttg
     3241 caaaatttca cgcggataga ctgctccagt tggattattt ggattaatga ggacaattgc
     3301 ttttgttttg gatgtgattt ttgatttgat atcatcaata tcaggatacc aattagcttc
     3361 ctcatcacaa atataatgca cagcatttcc accagcaagg ctaacacaag ctgtccataa
     3421 cgggtaatct ggcattggaa ctagaacctc gtcatcatta tcaagcaaag cttgcattga
     3481 catagagatt ccctcactaa caccattaac aatgtatata tcatccatgt caacatggat
     3541 gttttgtaac tggtaatact gcataacagc cttacgagct gaaaaaatac ctttcgaatc
     3601 agagtaacct tctgattcac gcgcattcgt aattaaatca cgaatgactt catctggcgc
     3661 ttcgaagcca aaggcagctg gatttccagt gtttaaccgc aatatctttt caccatttgc
     3721 cctcatgcgg tccgcctctt ctaaaacagg gcctctaata tcataagcca catgttccag
     3781 cttcattgat ttatcaaata tcttcataaa aatacctcgt atcttccatt ataaaacaaa
     3841 tattctcaaa attgaaacat ttactgcggt ttatcaagcg attaagttaa tattaagcta
     3901 tttcagaaaa ctaacataaa acgctttcat ttttctagaa aaaacgttat aatatagcta
     3961 taaaatgaat aaccaacata tctaacttac tcatcactca ctccttctga taagatggct
     4021 agttgagtac aagaggttat ctctttttgg ttatatttag aggaggacag gcttatgtca
     4081 caaaaatatg aacgcatcct tgtcgctatt gatggatcaa cagaatctga acttgctttt
     4141 gaaaaggcag ttaacgttgc tcttcgcaat gattctgaat taatcttgac acatgttata
     4201 gatacacgtg ctttacaaag ctttgcgaca tttgatactt atatctatga aaaattagaa
     4261 aaagaagcaa aagatgtcct tgaggaatat gaaaaacaag ctcgtgaaaa aggagcagat
     4321 aaagttagac aagttattga atttggtaat cccaaaacat tacttgcaca tgatattcca
     4381 gaaaaagaaa aagtagactt gattatggtt ggtgcaacag gtcttaatac ctttgagcgt
     4441 ttccttatcg gatcttcctc agaatacatc cttcgtcacg ctaaagtaga tttactcatt
     4501 gtacgtgatc ctaataaaac aatgtaaatt taaatacgct cactttctaa aatatcctga
     4561 gttatattaa ctcaggatat ttttattttc gatttaagga tttctgaatt tggctatcta
     4621 attcacgtcg aattgatggc tcaggagcga aatgttcttc ccagtcgtct ggttttaaaa
     4681 ttttatgagt aacaggatca aaatgagctt tgccatctgg aaagatttta cccatattgg
     4741 cctcatgtac tgtatcaaaa agaggttttg ggtcgactcc cataagaaca aaggagccat
     4801 aagtcagata aagtaaatct gttaaggcat ccacttctcc cactaatggt gtttctggat
     4861 ggtctttaga aataacctta tctcttgctt tatcaatagc taagtgtaaa ttacggatag
     4921 attggtcaaa tactttttgg ttaccttgac tagcagcata caagaactca acgatttctt
     4981 ctactttaaa gtctgacctg aaaccagctt ccttactgtc atataagcgt ggcgtttcaa
     5041 tagtttcacc atccattaat agatggaagg attttacttt attgaaattc tcgtcacgac
     5101 tactgaatgt tttttcaatc tcaaattgaa taagtccata atgggctaat gccttagaga
     5161 taccatcatc attattgtca gcagttgtat aacgtgcatt ttctttcact tgctgactgg
     5221 cgttccccat ggcaacacca atgccaactt gagataacat ctcaatatca ttatcagaat
     5281 ctccaaatgc tatcacttcg gataaatcaa aaccatagcg ttgaccaaca cgttcaattc
     5341 ccttaacttt tgactgctct tcagaaatga catccattga gtaaggactg cttcttgtta
     5401 atttaacacg aggaaattgt ttttgaattc gctctgattc gccctctgtt gcaactaaaa
     5461 cgacctgata aataggttct cgtaagataa tcatgaggct atcaatattt tgaggtttaa
     5521 tccgacgaat aaaatgctta aagcttcgtt caatcgcttt tgcccatttt ctcggaacaa
     5581 ggctagatac aatctgcccc aaacggctag ttcctagacc aataatacct gaaccgagta
     5641 atcccgaggc tgttcctaat gaaatttctc ttcttcttgc tccagcatat cgaatcagac
     5701 gatagacagt tgtcttagat aattgattgg tataaataat ttcacttcta ctatagatat
     5761 attgaccatt ataggttaca gcaaaatcta aaccaaggtt ttctaaaaaa ggttgtacaa
     5821 aactaggacc tcgccccgtt gctaaaccga ctagtattcc ttggtctttc aaatttcgaa
     5881 ttgctttaat tgttgacttt tgtacattct tacgatcatt aagaagtgta ccatcaatat
     5941 caaaaaatac tgccttaatt gccactgggt caacatcccc tcttttttat gataaaataa
     6001 attatatcac aacttgaaag aagtataaaa tcaatgaaaa aaatccttgt tctacataca
     6061 ggtggaacga tttccatgaa tgctaacgag aagggacagg tcatgtccag cgccgataac
     6121 ccaatgaaat acgttgattt atcattagat gaccttgatt tgactgttgt agattttcta
     6181 aatcttccca gtcctcaaat cacccctcat catatgcttg atatctatca ctatctgaaa
     6241 caacatgcca gtaattttga tggtgttgtc attacgcatg ggactgacac tttagaagaa
     6301 acagcatatt ttttagacac aatgatactc cctaaaatcc caatcatcat tactggtgct
     6361 atgagaagca caaatgaact tggtagcgat ggcgtttata attacctatc agccctccgt
     6421 gtcgctaata gtactaaagc tgccgacaag ggggtgctag ttgtcatgaa tgatgagata
     6481 catgctgcta aatatgtcac aaaaacacat acaacaaacg tttcgacctt ccaaacccct
     6541 acacatgggc cccttgggat tattatgaaa caagacctgc ttttctttaa agcaactgaa
     6601 gagagagttc gttttgattt agataaaatt acaggaactg ttcctatcgt gaaagcatat
     6661 gctggcatgg gtgatagtgg tatcattagc tttttaaatt ctcaaaatat tagcggatta
     6721 gttattgaag cactcggtgc agggaatatg cccccaaaag ctgcacaaga aatcgaagaa
     6781 ttaattgagc aaggtgtccc tgttgttcta gtgtctcgct gttttaatgg tattgctgag
     6841 ccagtttacg gatatgaagg tggcggagca agattacaag aatctggtgt catgtttgtt
     6901 aaagaactaa acgctcccaa agccagactc aaattactca ttgcactcaa tgcaggtctc
     6961 acagggcaaa atctaaaaga ctatattgaa ggttgataaa aaactctaga aatatttcta
     7021 gagtttttta ttaattttca gtttgtaata ctctctcaac actgctaata ggcatttctt
     7081 gattcgttat taattgaaat gcttctgctc cttgataaat cagcatccct agaccattat
     7141 aagcatgttt cactccattt tgtctaacaa atcgtaacaa tgctgtttct ttaggcttgt
     7201 aaaccaagtc gacaactact aaattgggtg ttaaaatttc tggatcgtta attaaactat
     7261 aattatctaa tggcctcatt cctaaactag tagcatcaat ataaaaatga ctagttctaa
     7321 tagcgtcttt aaatgctgtc ttattttcta gataatcaac gactaccttt atttgaaact
     7381 gttttttaat tttatctgat aagtcaatga ccttatcgta atttgagcta ttacgattaa
     7441 ataatctaat ttccgcaact ccctccatag ctgcttgaac tgcaactgct ttacctgaac
     7501 caccaatacc agctattgta actattttat ttttagcact gaaaccttga gctgctaaag
     7561 ctttaaaaca accaatgcca tctgtcatat ggcctactaa acgtccggtt ccaccttgat
     7621 taacgatagt atttacagca cccactaatt tagcttgagg agataaatca tctagcaaag
     7681 ggataacact ctgtttaaat ggcattgaaa cattaacacc acgaataccc aatgccctga
     7741 cacctcgaac agcttctgtt aatttaccct cttctacttc aaatgtcaga taggcataat
     7801 tcatgttttt ttcttgaaaa gaggtattcc acattaacgg ggatagagag tggcgtgcag
     7861 gattggcaat tagaccagtt agtaaagttt ccccatttaa tgaattcatt gtataacctt
     7921 ctttcttaac aatttatttc agttcatttt accaaaaact gactcaatta aaaagagagt
     7981 atgtaattta tactctctcc cttagtttaa ggcaatatat tacctgcact acggtaaata
     8041 tcataccatt cttcacgggt taaagcgata tcattagcct tatcaattgc ctttaaacga
     8101 gatggattca ttgaaccaac aatcgcctgt atttgggcag gatgacgtaa aatccatgca
     8161 atcacaatag cttcgctgct aacattgtag ttttttgcca gtttagctat ggtttcattt
     8221 aattccttat aatccggatg attaacaaat aaaccacgag ataaatcaat ttgaaatgga
     8281 gaccaagctt gaatggtaac tcttttgaga cgacaataat caacaatgcc atcatcatgt
     8341 tcaattgaag ctttatttaa catgttaaca tttaaccctg catcaaacat tggtgtatgt
     8401 gctggcgata attgaagttg attcactgct aagggctcgt caaggtagga ttgtaacaat
     8461 tccatttgaa accggttttg attgctaaca ccaaagtgtt ttactttacc ttctgccctt
     8521 agtttagtaa atgcttcagc tacttcttca ggctcaacta atacatctgg acgatgaaga
     8581 atgagaaaat ctaaatattc tgtccctaat ctttctaaaa taccatctac tgatgataaa
     8641 atatactctt tagaaaaatc aaagtaacct tctcgtatcc cacatttaga ttgaataatc
     8701 attttgtcac gattgacatt taaatgtttg attgcatctc gaaagcgtaa ttcagatagc
     8761 cctcccccat aaatatcagc atgatcaaaa aaattaatac ccaaatctaa tgctgttcca
     8821 actacttctt cagcttgttt tccttccaag tcactcattc tcatacaacc taaagcaatt
     8881 cgagttgctt gaatgcctgt ttgtccaatg ttttgtaaca tatgcacccc attctaatct
     8941 gtttgtatta acagaaagat taatcaattc attataacaa aatttctatg aaaaggctta
     9001 ctattacaaa tgcaaaaagc tgagtaaatt taactcaact ttttttctga tactcattat
     9061 tgactaaata agtcggtaag aaaaatctat tctgaaaagt actattataa attacagtaa
     9121 tcattattgt cataacatac ctccacatct gtgataggaa tatcatatga cagcaagctt
     9181 aaactgagct tagcacagcc ttaatagaaa cttaatcaaa atctgaatgt tggcgtaaat
     9241 tatctagaat aagtgcccat tctgtatttt ctttccaatt attatctttg acaatatcac
     9301 tagcaacacg tctggcttct tctaaaatag gataatcctc aacgatatca gctacttgaa
     9361 attctggaat tccagactga cgagtaccaa agatttcacc agaaccacgc atttttaaat
     9421 ccgactcagc taaaacgaaa ccatctgtcg tttctgtcat gattgtcatt cgttttttcc
     9481 cagaatcagt tttgggatta gccactaaaa cagcgtatga ttgtttatat ccacgaccaa
     9541 cacgcccacg aagttgatgt agctggctta atccaaaacg atcggcatcc ataataatca
     9601 tgatcgttgc atttgggaca tttaccccta cttcaataac tgttgtagat actaaaatat
     9661 gacttttttt atccttgaaa tcttgcatta tagcatcttt ttcatcattt ttcatacgtc
     9721 catgtacaag agcaacctta gcaattcctt caaaataagt agataattca gcatgcaatg
     9781 ctactgcatt ctttaaatct aaagcttctg actcttcaat caacggtgaa atgacataca
     9841 cttgagcatc tttttgcaat tcacctttta cccattccaa cacagtacct agctgctcgt
     9901 gtttcaccca gcgtgtaata ataggtttac gaccggcagg taattcatca ataatagaaa
     9961 catccatttc tccaaaagct gtgattgcta gagttcgggg aattggggtg gctgtcatca
    10021 ttaaaacatc aggattttct cccttttcac ggaaaattct acgctgttta acaccaaaac
    10081 gatgttgctc gtctgtaatt acaagcccca gtttatggta ctgtaccgag tcctggataa
    10141 gagcatgagt tcctacaatc atatcaaccg agccatttgc aatagctgct aaaaccgtac
    10201 gcttaacagc tgctttcata cccgaagtta atatagcgat tgataaatct ggaaataact
    10261 cttgcagact aatgtagtgt tgttcagcta ggatttccgt tggaaccatc aaagccgatt
    10321 gaaaacctgc tgtataagct gcatacattg atagaccagc aatgactgtc tttcctgatc
    10381 ctacatctcc ttgcaataaa cgattcatat gagctcccga tgacatatca gataatattt
    10441 catctaaaga gcgcttttga gcattcgtta gaataaaagg taaagaggaa atttttgtct
    10501 ccatagcatg tttactataa agaataggca aaccatttgt ttcagattta ttttcggatt
    10561 tcaaaacttg aaggttcatt tgaaagtaaa ataattcttc aaatttaatc cgacggagcg
    10621 cttgcttata ctctgtgata tcttttggga aatgcatagc taaacaagcc tgactacgac
    10681 ccatcaatcg gtatttttcc aataaagtag ctggtaaatt ttccttcaat tccaaatgcg
    10741 cactgatttc aaaagctgac ttaatagctt taatcaaagt agactgtgaa attccctgag
    10801 ctacgcgata aacaggttgc atgtcatctt caacttgagc taaaaccttc atcccagtaa
    10861 tagctgattt agttgcatcc catttaccaa agacagctac ctcttgacca agttctattt
    10921 tatcagctaa atagggttga ttaaaaaaac taacatttaa gacagcttcc ccttgacgca
    10981 acttaaaact taaacggtta cgtttaaaac cataatattg tacattagct ggagtaacga
    11041 ctaggcctgt aatgactgct ttttcaccat ccactaaat
//
LOCUS       DATRTN010000060        10868 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.060, whole genome shotgun sequence.
ACCESSION   DATRTN010000060 DATRTN010000000
VERSION     DATRTN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10868
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.060"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            288..1298
                     /gene="nrdF"
                     /locus_tag="VBE22_001451"
     CDS             288..1298
                     /gene="nrdF"
                     /locus_tag="VBE22_001451"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO7202900.1"
                     /translation="MQSYYDRSQSPLDYALSEKAFPMRSVNWNKLNDDKDLEVWNRVT
                     QNFWLPEKIPVSNDLNSWRTLDADWQQLITRTFTGLTLLDSVQATVGDIAQIKHSQTD
                     HEQVIYANFAFMVAIHARSYGTIFSTLCTSQQIEEAHEWVVDTESLQARSRILIPFYT
                     GDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGY
                     KYQQKVAKLSVEKQAEMKTFVFDLLYQLIDLEKAYLYELYDGFDLAEDAIRFSIYNAG
                     KFLQNLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYIMGITEETLDEDW
                     EF"
     gene            1299..1712
                     /gene="nrdI"
                     /locus_tag="VBE22_001452"
     CDS             1299..1712
                     /gene="nrdI"
                     /locus_tag="VBE22_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO7202901.1"
                     /translation="MDSTVKVVYFSSKSNNTHRFVQKLGCSNQRIPSDGSSILVTEDY
                     ILIVPTYAGGGDDTKGAVPKQVVQFLNVRQNREHCQGVISSGNTNFGDTYAIAGPIIA
                     RKLNVPLLHQFELLGTQEDVTRVKELLCQFTRKDK"
     gene            1714..3882
                     /gene="nrdE"
                     /locus_tag="VBE22_001453"
     CDS             1714..3882
                     /gene="nrdE"
                     /locus_tag="VBE22_001453"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO7202902.1"
                     /translation="MTQSDAYLSLNAKTRFRDRTGNYHFASDKEAVEQYMIEHVEPNT
                     MAFTSLIEKLDYLVSNNYYESDLLKQYNLEFICQIFEHAYAKKFTFLNFMGALKFYNA
                     YALKTEDNRYYLEHYEDRVVMNALFLAAGDEKAAYDLVDDMLANRFQPATPTFLNAGK
                     KRRGEYISCYLLRIEDNMESISRAISTSLQLSKRGGGVALCLTNLREFGAPIKGIKNQ
                     ATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKS
                     LSLGLVIPDITFELAKANKDMALFSPYDIERVYGKPMSDISITEEYETLLANADIRKT
                     FISARKLFQTIAELHFESGYPYILFEDTVNAKNPHKKEGRIVMSNLCSEIAQVNTASQ
                     FSEDLTFTKVGHDVCCNLGSINIARAMDQAADFEKLIANSIRALDRVSRTSDLDSAPS
                     IKKGNAANHAVGLGAMNLHGFLATNHIYYDSQEAIDFTDCFFYAMAYYAFKASNHLAK
                     EKGTFEGFSESSYADGSYFYQYTEQNFEPKTQRVKNLLAEYGLTLPSQEDWRKLVQSI
                     KEIGLANAHLLAVAPTGSISYLSSCTPSLQPVVSPVEVRKEGALGRVYVPAYKIDADN
                     YVYYKKGAYEVGPEAIINIAAAAQKHVDQAISLTLFMTDQATTRDLNKAYIQAFKQKC
                     ASIYYVRVRQDILEGSESYDDDMLDDFTSSDLEDCQSCMI"
     gene            3945..7112
                     /locus_tag="VBE22_001454"
     CDS             3945..7112
                     /locus_tag="VBE22_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEO7202903.1"
                     /translation="MTKKHLKTLALALTTVSVVTYSQEVYGLEREESVKQEQTQSASE
                     DDWFEEDNERKTNVSKENSTVDETVSDLFSDGNNNSSSSKTESVVSDHKQVPKAKPGV
                     TQEASNSSNDASKVEVPKQDTASKKETIETSTWEAKDFVTRGDTLVGFSKSGINKLSQ
                     TSHLVLPSHAADGTQLTQVASFAFTPDKKTAIAEYTSRLGENGKPSRLDIDQKEIIDE
                     GEIFNAYQLTKLTIPNGYKSIGQDAFVDNKNIAEVNLPESLETISDYAFAHMSLKQVK
                     LPDNLKVIGELAFFDNQIGGKLYLPRHLIKLAERAFKSNRIQTVEFLGSKLKVIGEAS
                     FQDNNLSNVMLPDGLEKIESEAFTGNPGDEHYNNQVVLRTRTGQNPHQLATENTYVNP
                     DKSLWRATPDMDYTKWLEEDFTYQKNSVTGFSNKGLQKVRRNKNLEIPKQHNGITITE
                     IGDNAFRNVDFQSKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASEDLEEIKEG
                     AFMNNRIGTLDLKDKLIKIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGAHHLMFIG
                     NKVKTIGEMAFLSNKLESVNLSEQKQLKTIEVQAFSDNALSEVVLPPDLQTIREEAFK
                     RNHLKEVKGSSTLSQIAFNAFDQNDGDKRFGKKVVVRTHNNSHMLADGERFIIDPDKL
                     SSTMVDLEKVLKIIEGLDYSTLRQTTQTQFREMTTAGKALLSKSNLRQGEKQKFLQEA
                     QFFLGRVDLDKAIAKAEKALVTKKATKNGHLLERSINKAVLAYNNSAIKKANVKRLEK
                     ELDLLTDLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSD
                     TIGEGQKDAYGNPVLNVDEDNEGYHALAVATLADYEGLYIKDILNSSLDKIKAIRQIP
                     LAKYHRLGIFQAIRNAAAEADRLIPKTPKGYLNTVPNYRKKQVEKNSKPVDYKTPIFD
                     KVLPNEKVDGDRVSKGHNIKAETNNSVAVTPTRSEQQLHKSQSDVNLPQTSSKNNFIY
                     EILGYVSLCLLFLVTAGKKGKRARK"
     gene            7125..7514
                     /locus_tag="VBE22_001455"
     CDS             7125..7514
                     /locus_tag="VBE22_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO7202904.1"
                     /translation="MITQEMKEIINSQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGAYYEAAKKWAEGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEIT"
     gene            complement(7558..8049)
                     /locus_tag="VBE22_001456"
     CDS             complement(7558..8049)
                     /locus_tag="VBE22_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO7202905.1"
                     /translation="MLSFQIVEKPAMILAGVTLENVKSNQECPKLWEKLHKQYNPSKL
                     KEKGIQQAIGICKTQPDFRFDYSATYRVETSVQAPKGLEIIRIPSATYAVISVKGPMP
                     SSLQETWRKIIQGFFQENNLKPANSPNLEIYSSQHPQDTDYQMEIWLAIADITNKSKT
                     NLI"
     gene            complement(8288..8410)
                     /locus_tag="VBE22_001457"
     CDS             complement(8288..8410)
                     /locus_tag="VBE22_001457"
                     /inference="COORDINATES: protein motif:HMM:NF021585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEO7202906.1"
                     /translation="MLRDYLEDMLKKEGKEVYPFSRLASHSANRWYLYETPWRY"
     gene            complement(8410..8571)
                     /locus_tag="VBE22_001458"
     CDS             complement(8410..8571)
                     /locus_tag="VBE22_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEO7202907.1"
                     /translation="MLTIQEMKDTTFELQENFRRLNYPIKQVAKDLQLNISEVESLLS
                     LDVTYPGDV"
     gene            complement(8658..8975)
                     /locus_tag="VBE22_001459"
     CDS             complement(8658..8975)
                     /locus_tag="VBE22_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEO7202908.1"
                     /translation="MKEKQTAGRRQLEEFAPEFARYNDDILFGEVWAKEDHLTDKTRS
                     IITISALISGGNLEQLEHHLQFAKQNGVTKEEIADIITHLAFYVGWPKAWSAFNKAKE
                     IWI"
     gene            complement(8999..9394)
                     /locus_tag="VBE22_001460"
     CDS             complement(8999..9394)
                     /locus_tag="VBE22_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO7202909.1"
                     /translation="MQETTKSIFPKGEKNPYGEFFIGQSYLAALAKSPDGNVSVGNVT
                     FEAGCRNNWHVHLDGYQILLVTEGSGWYQEEGKEAVSLKPGDVIVTDKGVRHWHGAKK
                     DSEFAHIAITAGKSEFYEAVSDEEYSRLG"
     gene            complement(9404..9793)
                     /locus_tag="VBE22_001461"
     CDS             complement(9404..9793)
                     /locus_tag="VBE22_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7202910.1"
                     /translation="MHIKEVSKKTGITSDTIRYYERINLIPPIHRSESGIREFTQNDI
                     DILEFVRYFRGAGVSVESLIDYIHLVEKGDSTIPARLEILKDERDNLEDRLQGLQEAL
                     NRLNHKIDNYQNKVVPREQELFDPTTK"
     gene            complement(9809..10845)
                     /locus_tag="VBE22_001462"
                     /pseudo
     CDS             complement(9809..10845)
                     /locus_tag="VBE22_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
BASE COUNT         3536 a         1858 c         2027 g         3447 t
ORIGIN      
        1 cattatgatg aaaacacaag atatagtggt gatatatgtt gaaagacaca aaactttgtg
       61 ttagtatact gtttaagcag atctttaata aaggcaaccg acaaaaaagt ttaatgtgac
      121 ttaacaaaac agtggttttg aagcaagagt tagcttctta ccagaatgaa aaaacatcaa
      181 gaagaacatt ccaatagttg tgtgatttta acaaaaatcc agtcctcttg ggaggctttt
      241 ttggtgctta aaactcgctt aattttaata tttaggagac tagtttaatg caatcatact
      301 atgatcgttc tcaatcgcca cttgactatg ccttgtcaga aaaagctttt cctatgcgtt
      361 ctgttaattg gaataagcta aacgatgaca aagacttaga ggtatggaac agagtaactc
      421 aaaatttctg gttacctgaa aagatcccag tttctaatga ccttaattct tggagaacat
      481 tggatgctga ttggcagcaa ctaattactc ggacttttac tggtctaacc ctattagatt
      541 ctgtgcaggc tacagttggt gatattgctc agattaagca ttctcagaca gatcatgaac
      601 aagttattta tgctaacttt gcctttatgg tagctattca tgcgcgatcg tatggaacga
      661 ttttttcaac cttatgcact agccaacaaa ttgaggaagc acatgaatgg gtagtggata
      721 ctgaaagtct acaagctcga tcacgtatac ttattccttt ttatactggt gacgatcctt
      781 tgaaatctaa agttgctgca gccatgatgc caggattttt gttatatggt ggcttttacc
      841 ttccttttta cctttcagcc agaggaaaat taccaaatac gtctgatatt attcgtttaa
      901 ttttacgaga taaggtaatt cataactatt acagtggcta taagtatcaa cagaaagttg
      961 ctaaattatc tgtagaaaag caagccgaaa tgaagacatt tgtctttgac ctcctctatc
     1021 agttgattga tttagaaaaa gcctatcttt acgaactata tgatggtttt gatttggctg
     1081 aagatgcaat caggtttagc atttacaatg ctggtaaatt tttacaaaat ttaggatatg
     1141 attctccatt tacagaagaa gagactcgta tatctcctga ggtttttgca caactttcgg
     1201 cgcgtgcaga tgaaaatcat gatttcttct caggaaacgg ctcttcatat attatgggga
     1261 ttacagaaga aacattggat gaagattggg agttttaaat ggatagtaca gtgaaggtgg
     1321 tctatttttc atcgaaatct aacaataccc atcgttttgt tcaaaagttg ggttgttcta
     1381 accagcgtat tccatcagat ggtagcagta tattggttac tgaggattat attttgattg
     1441 tgcctactta cgctggagga ggagatgata ccaaaggcgc agttccaaaa caggttgtac
     1501 agtttttaaa tgttcgacaa aatcgggaac attgccaagg ggttatttct tctggaaata
     1561 ccaattttgg tgacacttat gccattgctg gacctattat tgctcgaaaa ttaaatgttc
     1621 ctttgcttca tcaatttgaa ttactgggaa cacaagaaga tgttacaagg gtaaaggagt
     1681 tactttgcca attcactaga aaggataaat aagatgacac aatcagatgc atatctctcg
     1741 ttgaacgcga agacacgctt tagagatcgc acaggtaatt atcattttgc ttcggataaa
     1801 gaggctgttg aacaatatat gatagaacat gttgaaccta atacgatggc gttcacatca
     1861 ctaattgaaa agctagatta tttggtttct aataactact atgaatcgga ccttctaaaa
     1921 caatataacc ttgagtttat ttgccaaatt tttgagcatg catacgctaa gaaatttact
     1981 tttctaaatt ttatgggggc tttaaaattt tataatgctt atgctcttaa aacagaagac
     2041 aatcgttatt atttggaaca ctatgaagat cgtgttgtaa tgaatgctct gtttttagct
     2101 gctggtgatg aaaaagctgc ctatgatcta gtagatgaca tgctagcaaa tcgctttcaa
     2161 ccagcaacac ctaccttttt aaatgcaggg aaaaaacgtc gtggtgaata tatatcttgc
     2221 tatttgttgc gcattgaaga caatatggaa tctatttctc gtgctattag tacaagcctt
     2281 cagttatcaa aacgaggtgg tggggtagca ctttgcttaa ccaacttacg ggaatttggt
     2341 gctccaataa aaggtattaa aaatcaagct actggtattg tacctgtcat gaaattacta
     2401 gaagattcgt tttcttacgc caatcaatta ggacaaagac aaggagctgg tgcagtttac
     2461 ttacatgctc atcaccctga agtgttaaca tttttagata ctaaacgtga gaatgccgat
     2521 gagaaaattc gaattaaatc tctctcccta ggacttgtta ttcctgatat tacttttgaa
     2581 ttggctaaag caaataaaga tatggccttg ttttcaccgt atgacattga gcgagtatat
     2641 ggtaaaccaa tgtctgatat ctctatcaca gaagaatatg aaactttatt ggccaatgcg
     2701 gatattcgta aaacctttat tagtgcacga aaactatttc aaacgatagc agagcttcat
     2761 tttgaatctg gttatcctta tatcttattt gaagatactg taaatgctaa aaaccctcac
     2821 aaaaaagagg gacgaattgt tatgtctaat ttatgttctg agattgcaca agttaataca
     2881 gccagtcaat tttcagaaga cctgactttc actaaggttg gacatgatgt ttgttgtaat
     2941 ttaggttcta ttaatattgc gcgtgctatg gatcaagcgg cggattttga aaaattaatt
     3001 gctaatagca ttagagccct tgatcgtgta tctcgaactt ctgatttaga tagtgctcca
     3061 tctattaaaa aaggtaatgc agctaatcat gctgttggtt taggtgctat gaatttgcat
     3121 ggatttttgg cgactaacca tatttattac gattctcaag aagctattga ttttacagat
     3181 tgcttctttt acgctatggc ttattatgcc tttaaagctt ctaaccattt agcaaaagaa
     3241 aaaggaacct ttgaaggctt ctcggaatca agttatgctg atggcagtta tttctatcaa
     3301 tataccgaac aaaactttga gccaaaaaca cagcgtgtta aaaatctact agctgagtat
     3361 ggcctgactc taccaagcca agaggattgg agaaagcttg tgcaaagtat taaagagata
     3421 ggattagcta atgcgcattt attagctgtt gctccgacag ggtcaatcag ttatctttct
     3481 tcttgtactc cgagcctcca accagttgta tcacctgtcg aagtacgcaa ggaaggagca
     3541 ctggggaggg tttatgtacc tgcttataag attgatgcag ataattatgt ctactacaaa
     3601 aaaggagctt atgaagtggg acctgaggcg attatcaata ttgcagctgc cgctcaaaaa
     3661 cacgttgatc aagctatttc gctaacgctt ttcatgacag atcaagcaac tacgcgagat
     3721 ttaaataaag cctatattca agcatttaaa caaaaatgtg cctctattta ttatgtacga
     3781 gtgagacagg acatcctaga aggtagcgag agttatgatg atgatatgct ggatgatttc
     3841 acttcatcag acttagaaga ctgtcagtcc tgcatgattt aactaaatat actcaatcag
     3901 aagattaaga gaaatatatt ggcttttaga aaggctttat tgtaatgaca aaaaaacatc
     3961 ttaaaacgct tgccttggca cttactacag tatcagtagt gacatacagc caggaggtat
     4021 atggattaga aagagaggaa tcggtcaaac aagaacaaac ccagtcagct tcagaagatg
     4081 attggttcga agaagataat gagaggaaaa caaatgtttc taaagagaat tctactgttg
     4141 atgaaacagt tagtgattta ttttctgatg gaaataataa tagctctagt tctaaaaccg
     4201 agtcagtggt aagtgaccat aaacaagtcc ccaaagcaaa accaggggtt acacaggaag
     4261 caagcaattc tagtaatgat gctagcaaag tagaagtacc aaaacaggat acagcttcaa
     4321 aaaaggaaac catagaaaca tcaacttggg aggcaaaaga tttcgtaact agaggggata
     4381 ctttagtagg tttttcaaaa tctggaatta ataagttatc tcaaacatca cacttagttt
     4441 taccaagtca tgcagcagat ggaactcaat tgacacaagt agctagcttt gcttttactc
     4501 cagataaaaa gacggccatt gcagaatata caagtaggct aggagaaaat gggaaaccga
     4561 gtcgtttaga tattgatcag aaggaaatta ttgatgaggg agaaatattt aatgcttacc
     4621 agttgactaa gcttactatt ccaaatggtt ataagtctat tggtcaagat gcttttgtgg
     4681 acaataagaa tattgctgag gttaaccttc ctgagagtct cgagactatt tcagactatg
     4741 cttttgctca catgtcttta aaacaagtaa aattaccaga taacctaaag gtcattggag
     4801 aattagcttt ttttgataac cagattggtg gtaagcttta cttgccacgt cacttgataa
     4861 aattagcaga acgtgctttc aaatctaatc gtattcaaac agttgaattt ttgggaagta
     4921 agcttaaggt tataggagaa gcaagttttc aagataataa tctgagtaat gttatgcttc
     4981 cggatggact tgaaaaaata gaatcagaag cttttacagg aaatccagga gatgaacatt
     5041 acaacaatca ggtcgtattg cgcacacgga caggccaaaa tccacatcaa cttgcgactg
     5101 agaatactta cgtcaatccg gacaaatcat tgtggcgtgc aacgcctgat atggattata
     5161 ccaaatggtt agaggaagat tttacctatc aaaaaaatag tgttacaggt ttttcaaata
     5221 aaggcttaca aaaggtaaga cgtaataaaa acttagaaat tccaaaacaa cacaatggta
     5281 ttactattac tgaaattggt gataacgctt ttcgcaatgt tgattttcaa agtaaaactt
     5341 tacgtaaata tgatttggaa gaagtaaagc tcccctcaac tattcggaaa ataggtgctt
     5401 ttgcttttca atctaataac ttgaaatcct tcgaagcaag tgaagattta gaagagatta
     5461 aagagggagc ctttatgaat aatcgtattg gaactctaga cttgaaagac aaacttatca
     5521 aaataggtga tgctgctttc catattaatc atatttatgc cattgttctt ccagaatctg
     5581 tacaagaaat aggacgttca gcttttcgac aaaatggtgc acatcacctt atgtttatcg
     5641 gaaataaggt taaaacaatt ggtgaaatgg cttttttatc caataaactg gaaagtgtaa
     5701 atctctctga gcaaaaacaa ttaaagacaa ttgaagtcca agctttttcg gataatgccc
     5761 ttagtgaagt agtcttaccg ccagatttac agactattcg tgaagaggct ttcaaaagaa
     5821 atcatttgaa agaagtgaag ggttcatcta cattatctca gatcgctttt aatgcttttg
     5881 atcaaaatga tggggacaaa cgctttggta agaaagtggt tgttaggaca cataataatt
     5941 ctcatatgtt agcagatggt gagcgtttta tcattgatcc agataagcta tcttctacaa
     6001 tggtagacct tgaaaaggtt ttaaaaataa tagaaggttt agattactct acattacgtc
     6061 agactactca aactcagttt agagaaatga ctactgcagg taaagcgttg ttatcaaaat
     6121 ctaacctccg acaaggagaa aaacaaaaat tccttcaaga agcacaattt ttccttggtc
     6181 gcgttgattt ggataaagcc atagctaaag ctgagaaggc tttagtgacc aagaaggcaa
     6241 caaagaatgg tcatttgctt gagaggagta ttaacaaagc ggtattagct tataataata
     6301 gcgctattaa aaaagctaat gttaagcgct tggaaaaaga gttagacttg ctgacagact
     6361 tagtcgaggg aaaaggacca ttagcgcaag ctacaatggt acaaggagtt tatttattaa
     6421 agacgccttt accattgcca gaatattata tcggattgaa cgtttatttt gacaagtctg
     6481 gaaaattgat ttatgcactt gatatgagtg atactattgg cgagggacaa aaagatgcat
     6541 atggtaatcc tgtattaaat gttgacgagg ataatgaagg ttatcatgcc ttggcagttg
     6601 ccactttagc tgattatgaa ggtctttata ttaaagatat tttaaatagt tcccttgata
     6661 agattaaagc aatacgtcag attcctttgg caaaatatca tagattagga atttttcaag
     6721 ctatccgaaa tgcagcggca gaagcagacc gattgattcc taagacacct aaggggtacc
     6781 taaatacagt cccaaattat cgtaaaaaac aagtggagaa aaattcaaaa ccagttgatt
     6841 ataaaacgcc gatttttgat aaggttttac ctaatgaaaa ggtagacggc gatagagtgt
     6901 ctaaaggtca taatataaag gcggagacta ataattctgt agctgtaaca ccaacaaggt
     6961 ccgagcagca attacataag tcacagtctg atgtaaattt acctcaaaca agttctaaaa
     7021 ataattttat atacgagatt ctaggatacg ttagtttatg tttgcttttc ctagtaactg
     7081 ctggaaaaaa aggaaaacga gcaagaaaat aaggagaaat taatatgatt acacaagaaa
     7141 tgaaagagat tattaatagt cagttagcaa tggtcgcgac ggttgatgct aaaggtcaac
     7201 ccaatattgg cccaaaacgt tctatgcgtc tctgggatga taaaacgttt atttataatg
     7261 aaaatacaga tggtcaaact cgtattaaca ttgaagataa tgggaaaatt gaaattgcct
     7321 ttgttgatcg tgaacgtctt ttaggttaca ggtttgtggg aactgctgaa attcaaacag
     7381 aaggtgctta ttacgaagca gctaaaaaat gggcagaagg acgcatgggg gttccgaaag
     7441 cagttggtat tattcatgtc gaacgtattt ttaatttaca atctggtgct aatgcaggta
     7501 aagaaatcac ttaatataaa aaagcctcaa aaaggggctt ttttatatta agcaaggcta
     7561 gattaagttt gttttcgatt tattagtaat atctgcgata gcaagccaaa tttccatttg
     7621 ataatcagta tcttgtggat gttgagatga ataaatttct aagtttggcg agttagctgg
     7681 ttttagatta ttctcttgaa aaaatccttg tattattttt cgccatgttt cttgaagact
     7741 cgatggcatt ggacctttaa cagaaatgac tgcataggta gcgcttggga tcctaataat
     7801 ttctaaccct tttggggctt ggacacttgt ctctactcga taagtggcgc tgtaatcgaa
     7861 acgaaaatca ggttgtgttt tacaaatgcc aatggcttgc tggatacctt tttcttttag
     7921 ttttgatgga ttatattgtt tatgtaattt ttcccaaagt ttaggacatt cttgatttga
     7981 ctttacattt tctaaagtta ctccagcaag tatcattgct ggcttttcaa caatttgaaa
     8041 tgatagcata aaaaccttta ttctttatga ttccttaagt aattatagag tcgttaaaat
     8101 agtttctttt tagaataatt gtcttgttag caatcataat tttcttttgg aatgctatgc
     8161 ttcaaagaag aaatcttatg atgtagagta ttctctaaga ggctcttgtt ttatagcttt
     8221 tttctaaaaa gaatctattg actatttttg tttatttctc taaagatgat ggattaatct
     8281 ttagtcttta ataacgccat ggtgtttcat aaaggtacca acgatttgct gaatgagatg
     8341 ctaatctaga aaatggataa acttctttac cctctttttt caacatatct tctagataat
     8401 ctctcagcat caaacatctc caggataagt aacatcaaga cttaaaagag attcaacttc
     8461 actgatatta agttgcaagt ctttagcaac ttgtttaata ggataattta accttctaaa
     8521 attttcttgt aattcaaagg ttgtatcttt catttcttga atagtaagca tatcattctc
     8581 ctaatagttg gtttaataat tggttgatct ttctttttgg cttttgggaa agtatctccc
     8641 ttgctatgtc tgttatttta tatccaaatt tccttagctt tattaaaggc tgaccaagct
     8701 tttggccatc caacataaaa agcaaggtga gtgataatgt cagcaatttc ttctttagta
     8761 actccgtttt gtttggcaaa ttgaagatga tgctctaact gttcaagatt tcctccgcta
     8821 attaaggcag atatagtaat aatagaacgt gttttatctg ttaaatgatc ctcttttgcc
     8881 caaacctcac caaataaaat atcatcatta tatctggcaa attcaggtgc aaactcttct
     8941 aattgacgac gtccggctgt ttgtttttct ttcataatgg attcctcctt acttgaattt
     9001 aacctagtct tgaatattct tcatcactaa cagcttcata aaactcactc ttaccagctg
     9061 taatcgcaat atgtgcaaat tcagaatctt ttttagcacc atgccaatgt ctaacacctt
     9121 tatctgtaac gataacgtcg cctggtttta atgacactgc ctcttttcct tcttcttggt
     9181 accaacctga accttctgta acgagaagga tttgataacc gtctaaatga acatgccaat
     9241 tattacgaca gcctgcttca aaggttacat ttccaactga aacattccca tctggagatt
     9301 ttgctaatgc tgcgagatag gattgcccaa taaagaattc accatatgga tttttttcac
     9361 ccttaggaaa gatagactta gttgtttctt gcataagatt tcctcatttc gttgttggat
     9421 caaaaagttc ttgttctcta ggaacaactt tattttgata attgtctatt ttgtgattta
     9481 gacgattaag tgcttcttgg aggccttgaa gcctatcttc aagattgtcg cgttcatctt
     9541 ttaaaatttc tagcctagct gggatagtgg aatcgccttt ttcaaccaaa tgaatataat
     9601 caatgagact ttcaacggaa acacctgcgc ctctaaaata gcggacaaat tctaaaatat
     9661 caatatcgtt ttgtgtgaac tcacgaattc ctgattcact ccgatgaata gggggaataa
     9721 gattaattct ctcataatac ctgatggtat ctgacgtaat gccagtcttt ttactaactt
     9781 ctttgatatg catttttaac ctcgatattc agtcaacaat cactaaggat ttgatagcgt
     9841 cacgtttatc cattgcttca taagcctttt gaatatcatc taatacaaag gattttgtaa
     9901 aaactaagcc tgggttaatt ttatgagtta gaacagcatc taaaaggaca gatttatcaa
     9961 atgttgttac agaagcaatg ccacctctaa ggccgatatt tttccagaat aaattattgg
    10021 tattcatgtc tggattttgt gggataccaa cacgtcctat gacagcacca ggcctagcaa
    10081 tttgggtagc tgtatcaaca gactgctctg taccaacaca ttctaaaata gcatcggcac
    10141 ctgcttgatt ggttaaatct aaaacacgtt taacggcttc gtcaccccgt tcttcaacaa
    10201 tatccgttgc accaaaagtc aatgctagtt cttgcctatc tttgtgacga ctcattgcaa
    10261 tgatacagtt agcacctaac attttagcag cgataacacc acagagtcca acagcaccat
    10321 ctcccatgac aactaccgta tcaccttctt taacttctgc agttactgca gcatgataac
    10381 cagttgccat tacatctgat aaggtgagca gagagttaag agtctcatta tcataatcag
    10441 aaggttgtcc tgggatctta accaatgccc agtttgcatt agtataacgt agatattgac
    10501 cttggtatct acattttttg cagcttgatg atttgtaaaa tttccatcaa atccagcctt
    10561 acaagacgga cattggccac agccatgtgt aaagggaaca ataacaaaat caccttttga
    10621 cacgtcagtt actttagtac caacttcctc aacgatacca atagcctcat gacctgcaaa
    10681 agagccactt tcacgttttg aaataccacg ataccaccac aagtctgaac cacaaacaca
    10741 ggctctaaca attttgataa cagcatctgt ctcttgttca atgactggtt ttggtgtatc
    10801 agttataacc attttgccag gttcaataaa tgtagcaact ttcataacct aacctctttt
    10861 ctataact
//
LOCUS       DATRTN010000061        10449 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.061, whole genome shotgun sequence.
ACCESSION   DATRTN010000061 DATRTN010000000
VERSION     DATRTN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10449
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.061"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            34..519
                     /locus_tag="VBE22_001463"
     CDS             34..519
                     /locus_tag="VBE22_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202911.1"
                     /translation="MRETETHDHQALIQKLLVSIHYLTLFRDEIILVEKTPSLLGKHF
                     SIAIVQNELGEILSKIEALSKQKKLIRSIYWYDESSFKVMNKALAIVEEWIKGLDNLL
                     EFCQLQTVFQAILGDERAHVFGILIDVYTSLNIINTSLKEENSRPVSLSDLALGLKDE
                     D"
     gene            618..1271
                     /locus_tag="VBE22_001464"
     CDS             618..1271
                     /locus_tag="VBE22_001464"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO7202912.1"
                     /translation="MKHSSWHDLIKRELPNHYYNKINTFMDAVYESGIVYPPRDKVFN
                     AIQITPLENVKVVIIGQDPYHGPQQAQGLSFSVPDNLPAPPSLQNILKELAEDIGSRS
                     HHDLTSWAQQGVLLLNACLTVPEHQANGHAGLIWEPFTDAVIKVVNQKETPVVFILWG
                     GYARKKKSLIDNPIHHIIESPHPSPLSAYRGFFGSRPFSRTNHFLEEEGINEIDWLN"
     gene            complement(1337..1975)
                     /gene="plsY"
                     /locus_tag="VBE22_001465"
     CDS             complement(1337..1975)
                     /gene="plsY"
                     /locus_tag="VBE22_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEO7202913.1"
                     /translation="MNIIIMIIIAYLLGSIQTGLWIGKYFYQVNLRQHGSGNTGTTNT
                     FRILGVKAGIVTLTIDILKGTLATLIPIILGITTVSPFFIGFFAIIGHTFPIFAQFKG
                     GKAVATSAGVLLGFAPSFFLYLLVIFLLTLYLFSMISLSSITVAVVGILSVLIFPLVG
                     FILTDYDWIFTTVVILMALTIIIRHQDNIKRIRKRQENLVPFGLNLSKQKNK"
     gene            2089..4038
                     /gene="parE"
                     /locus_tag="VBE22_001466"
     CDS             2089..4038
                     /gene="parE"
                     /locus_tag="VBE22_001466"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEO7202914.1"
                     /translation="MAKQDITVTNYGDDAIQVLEGLDAVRKRPGMYIGSTDGTGLHHL
                     VWEIVDNAVDEALSGFGNRIDVIINKDGSITVTDHGRGMPTGMHAMGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEIIRDGAIYRQRFENGGKPVTTLK
                     KIGTAPKSKSGTSVSFMPDQSVFSTIDFKFNTIAERLKESAFLLKNVTLTLTDNRSEE
                     AEHLEFHYENGVQDFVEYLNEDKETLTPIMFFEGEEQEFHIEVALQYNDGFSDNILSF
                     VNNVRTKDGGTHETGLKSAITKSMNDYARKTGLLKEKDKNLEGSDYREGLSAILSILV
                     PEEHLQFEGQTKDKLGSPLARPIVDGIVSEKLTYFLMENGDLASNLIRKAIKARDARE
                     AARKARDESRNGKKSKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTAKAKMADIIKNEEINTMIHTIGAGVGPDFNIDDINYDKVIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEEGHVYIALPPLYKMSKGKGKKEIVEYAWTDIEL
                     EELRQKFGKGSLLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLM
                     GDKVPPRRQWIEDNVKFTLEENTVF"
     gene            4172..6631
                     /gene="parC"
                     /locus_tag="VBE22_001467"
     CDS             4172..6631
                     /gene="parC"
                     /locus_tag="VBE22_001467"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEO7202915.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGFRKSAKSVGNVMGNFHPHGDSSIYDAMVRMSQDWKNRETLIEMH
                     GNNGSMDGDPAAAMRYTEARLSEIAGYLLQDIDKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPKAKLDKLMEFLPGPDFPTGAI
                     IQGKDEIRKAYETGKGRVAVRSRTAIETLKGGKKQIIVTEIPYEVNKSVLVKRIDDVR
                     VNNKVPGIAEVRDESDRDGLRIAIELKKEADETIVLNYLFKYTDLQVNYNFNMVAIDD
                     YTPKQVGLSRILTSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVLSILDEVIALI
                     RASENKADAKENLKVSYEFSEAQAEAIVTLQLYRLTNTDIVTLREEEEELRQQITMLK
                     AIISDERTMYNVMKRELREVKKKFANTRRSELQELAETIEIDTASLIIEEDTYVSVTR
                     GGYVKRTSPRSFNASTVDELGKREDDELIFVSNAKTTQHLLMFTNLGNLAYRPVHELA
                     DIRWKDVGEHLSQNLVNFASNEEIIYAELVDDFTKETYFAVTSLGQIKRFERQEISPW
                     RTYKSKTAKYAKLKNVDDYVVTVAPIQLEDVILVTYNGYALRFSINDVPVVGSKAAGV
                     KAMNLKDSDHIVSAFIANTTSLYLLTHRGSLKRMAIDVIPTTSRANRGLQVLRELKSK
                     PHRVFKAGPVYLEDSSFEFDLFSSVSNHEGDTFVLEIMSKTGKVYDVDLSQWSFSERT
                     SNGSFVSDKISDEEVFSVKIK"
     gene            6744..7766
                     /locus_tag="VBE22_001468"
     CDS             6744..7766
                     /locus_tag="VBE22_001468"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEO7202916.1"
                     /translation="MTVNLDWDNLGFAYRKLPFRYISHFKDGKWDDGKLTDDATLHIS
                     ESSPALHYGQQAFEGLKAYRTKDGSIQLFRPDQNAERLQRTADRLLMPHVPTDKFIAA
                     VKSVVRANEEFVPPYGTGATLYIRPLLIGVGDIIGVKPAEEYIFTVFAMPVGSYFKGG
                     LTPTNFIVSKEYDRAAPNGTGAAKVGGNYAASLLPGKYAHEKQFSDVIYLDPATHTKI
                     EEVGAANFFGITKDNQFITPLSPSILPSITKYSLLYLAKERFGMEAIEGDVFVDELDK
                     FTEAGACGTAAVISPIGGIQNGDDFHVFYSETEVGPATRKLYDELVGIQFGDVEAPEG
                     WIYKVD"
     gene            7849..8079
                     /locus_tag="VBE22_001469"
     CDS             7849..8079
                     /locus_tag="VBE22_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEO7202917.1"
                     /translation="MSKKDKKIEIQISDAQVTVNGTKVDGYQLVIGKKIVGQIAELDS
                     KFAVIKGEQVSGFYKNLDQAMGNIIEEYNLNR"
     gene            8140..8220
                     /locus_tag="VBE22_001470"
     tRNA            8140..8220
                     /locus_tag="VBE22_001470"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8174..8176,aa:Tyr,seq:gta)
     gene            8226..8297
                     /locus_tag="VBE22_001471"
     tRNA            8226..8297
                     /locus_tag="VBE22_001471"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8258..8260,aa:Gln,seq:ttg)
     gene            8423..9625
                     /gene="rpsA"
                     /locus_tag="VBE22_001472"
     CDS             8423..9625
                     /gene="rpsA"
                     /locus_tag="VBE22_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEO7202918.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIDGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVVEESAAAARKEVFSNIEVGSVVTGKVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVTVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSAGQEVTVKVL
                     EVNSDAERVSLSMKALEERPAQAEGEKEEKRQSRPRRPRRQEKRDYELPETQTGFSMA
                     DLFGDIEL"
     gene            9698..9769
                     /locus_tag="VBE22_001473"
     tRNA            9698..9769
                     /locus_tag="VBE22_001473"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9731..9733,aa:Arg,seq:ccg)
     gene            complement(10038..>10449)
                     /locus_tag="VBE22_001474"
     CDS             complement(10038..>10449)
                     /locus_tag="VBE22_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEO7202919.1"
                     /translation="SLAELKQLFGGEPNEKFDTPAGNVTLKGYRWNVDDISITIQLLN
                     DSSIVRSISNFKFIRDANITTKDYNSLKNGMSYNKVKELLGEPDDISQAVSSDKEELQ
                     AAWISGIQSSDSDPGINLTFENDKLTNKQQHGLK"
BASE COUNT         3468 a         1640 c         2115 g         3226 t
ORIGIN      
        1 tttaccagtc agaaatagta aggatggttg agtatgagag aaaccgaaac acacgatcac
       61 caagcgctaa ttcaaaaatt gttagtcagt attcattatt taaccttgtt tcgtgatgaa
      121 attatattag tagaaaaaac cccatcttta ctggggaaac atttttctat tgctattgtg
      181 cagaatgaac taggtgagat actttctaaa atagaagctt tgagtaagca aaaaaaactg
      241 attcgttcta tttattggta tgatgaatcg tcatttaaag tcatgaataa ggctctggct
      301 attgtagaag aatggattaa gggcttagat aatcttttag aattctgtca attacagacg
      361 gtttttcaag caattttagg tgatgaacgt gcacatgtat ttggaatcct tattgatgtt
      421 tatacttctt taaacattat aaatacttct ttgaaagaag aaaattctag accagtgtcc
      481 ttatctgatt tggctctagg tcttaaggac gaagattaat aaaaaaacgt tactgctttg
      541 aatgaataaa gcagtaacgt tttttataaa atattgctat actagtaata cagatatcta
      601 aaagtaagga gttataaatg aaacattcta gttggcatga tttaataaaa agagaactac
      661 ccaatcatta ctacaataaa attaatacat ttatggatgc agtttacgaa agcggaatcg
      721 tttatcctcc aagagataag gtgttcaatg ccatacagat aacaccacta gaaaacgtaa
      781 aagtagtgat tattggccaa gatccttacc atggccctca acaagctcaa ggactctcat
      841 tttctgttcc agacaattta ccagctcctc cttcacttca aaatattcta aaagaattag
      901 cagaagatat tgggtcacgt tcacatcatg acttaacatc atgggcgcaa caaggagtgc
      961 tgctattgaa tgcttgttta acagttccag aacaccaagc taatggtcat gctgggctta
     1021 tatgggaacc ttttactgat gctgtcatca aagtggttaa ccaaaaggaa acaccagtag
     1081 tctttattct gtggggtggc tatgctcgaa agaaaaagtc gctaattgat aatcctattc
     1141 atcatattat tgagagtcct catccgagtc ccttatctgc ttatcgtggc ttctttggta
     1201 gcagaccttt ttctagaaca aatcatttct tagaagaaga aggaataaat gagattgatt
     1261 ggttaaatta gaagggaaat ctactctcga aacattgtta gttttataat aaaaaaccgc
     1321 tactagcggt tttttattat ttattttttt gtttacttag gtttaagcca aacggaacca
     1381 aattttcttg cctttttcga atacgtttga tattatcctg atgtcggata ataatcgtga
     1441 gagccattaa gataaccaca gtggtaaata tccagtcgta gtccgttaat ataaagccta
     1501 ctaatggaaa aattaagaca ctaagaatac ctacgacagc tactgtaata ctggataggg
     1561 aaatcatgct aaaaagataa agcgttaata aaaagataac taataagtaa aggaaaaaag
     1621 aaggtgcaaa tcctagcaaa accccagcgc ttgtagcaac ggctttccca cctttgaatt
     1681 gtgcaaaaat agggaaagtg tgacctataa tagcaaaaaa accgataaaa aatggagata
     1741 ctgtcgttat acctaatatg ataggaataa gtgttgctag agtccctttt aaaatgtcaa
     1801 tagtcaaagt gacaatgcca gcttttacac ctaaaatacg gaaggtattc gtggtgccag
     1861 tattaccact accgtgttga cgaagattga cttggtaaaa atatttacca atccaaagtc
     1921 cagtttggat agagcctaac agataggcaa tgataatcat aattataata ttcataccct
     1981 tattataaca taagcataaa agtctttttg ctatttttaa aaaaataatt ttgcaaaaac
     2041 agcaaaaaac ttgtaatata aaaaagatga aaaaatttgg aggtcacttt ggctaaacaa
     2101 gatataacag tgacgaatta tggcgacgat gctattcaag tcttagaagg attagatgct
     2161 gttcgtaagc gtccagggat gtatataggt tccacagatg gaactggttt acatcattta
     2221 gtgtgggaaa ttgtagataa tgcagttgat gaagctctat caggttttgg aaatcgcatt
     2281 gatgttatta ttaacaaaga tgggagtata actgttacag accatggacg cggtatgcca
     2341 actggaatgc atgctatggg aaaacctact gttgaagtca tcttcacggt gcttcatgca
     2401 ggtggaaaat ttggacaagg tggttataaa acatcaggtg gtctccatgg tgttggatca
     2461 tctgtagtta atgctttgtc ttcttggtta gaagttgaaa tcattcgcga cggagctatt
     2521 tacagacaac gttttgaaaa tggtggtaag cccgttacaa ccctgaaaaa aatcggcacg
     2581 gcaccaaaat caaaatctgg gacatctgtc agctttatgc cagatcagtc agtattttca
     2641 acgatagatt ttaagtttaa tacaattgca gagcgcttaa aggaatctgc ctttctctta
     2701 aaaaatgtta ctttaacctt aacagataat cgttcagaag aagcagagca tctagaattt
     2761 cattatgaaa acggtgttca agattttgta gagtatttaa atgaagataa agaaacgttg
     2821 acaccaatca tgtttttcga aggtgaagaa caagaatttc atatcgaagt tgctttgcag
     2881 tataatgatg gtttttcaga taatattctt tcttttgtca ataatgttcg tactaaagat
     2941 ggtggaacac atgaaactgg cttgaaatca gctattacaa agtctatgaa tgactatgca
     3001 cgtaaaacag gacttcttaa agaaaaagat aaaaaccttg aaggttctga ttatcgtgag
     3061 gggctatcag ccattttatc tattcttgta cctgaggagc acttgcaatt tgaaggccaa
     3121 actaaagata aactaggaag tcccttagca cgaccaattg ttgatggtat tgtttctgaa
     3181 aagttaactt actttcttat ggaaaatgga gatttagcat ctaatttaat ccgtaaggca
     3241 ataaaagcac gtgatgcgcg tgaagctgca cgtaaagctc gtgatgaaag tagaaatggg
     3301 aaaaagtcga aaaaagataa aggtctactt tctggaaaat taacccctgc acaatcaaaa
     3361 aatgctaaga aaaatgaact ctatttagtc gaaggagact ctgcaggtgg ctctgctaag
     3421 caaggtcgtg atagaaagtt tcaagctatt ttaccgttgc gaggaaaagt attgaataca
     3481 gctaaggcaa agatggctga tattataaaa aacgaagaaa ttaatacaat gatccatacc
     3541 attggtgcag gtgttggtcc tgattttaat attgacgata ttaattatga taaagttatc
     3601 attatgaccg atgctgatac tgacggagct catattcaga cactattatt aactttcttt
     3661 tatcgctata tgagacctct tgttgaagag ggacatgtct atattgcttt gccacctttg
     3721 tataaaatgt caaaaggaaa aggtaagaaa gaaattgttg agtatgcttg gacggatata
     3781 gagttagaag aattacgaca aaaatttggt aaaggctcac tattacaacg atataaaggg
     3841 cttggggaaa tgaatgccga tcaattgtgg gaaaccacta tgaaccctga gacaaggaca
     3901 ttaattcgtg taacaattga agatttagca agagcagaac gtcgagttaa tgtcttgatg
     3961 ggtgataaag ttcctccaag acgccaatgg attgaagata atgtcaagtt tactttagaa
     4021 gagaatacag tattttaata aagagtcttg gttattcatg ttttatagtg tacaagtaat
     4081 attgtatgcg ttagacaaga gagccaatca ttcatttcaa ataagtggaa aaataatact
     4141 aagccactcc aactaggaga aaattaaaaa tatgtctaat attcaaaata tgtcccttga
     4201 ggacattatg ggagagcgtt ttgggcgcta ttctaagtat atcattcaag aacgggcgtt
     4261 gccggatatt cgtgatggct taaaacctgt tcaacgtcga atcctttatt caatgaataa
     4321 agatggtaat acttttgaaa agggatttcg caaatctgca aaatcagtcg gtaatgttat
     4381 ggggaatttc catcctcatg gggattcctc tatctatgac gcgatggttc gtatgtctca
     4441 agattggaaa aatcgtgaga cattgattga aatgcacgga aataatggtt ctatggatgg
     4501 agatcctgcg gcggctatgc gttatacgga agcccgtcta tcagaaattg ctggttatct
     4561 tcttcaagat attgataaga ataccgtacc ctttgcctgg aatttcgatg atacagagaa
     4621 agaaccaact gttttaccag ctgcctttcc caatctttta gtcaacggag caacaggaat
     4681 atctgcaggc tatgcaacag atattccacc acataattta gcagaagtca ttgatgctgt
     4741 cgtgtacatg attgatcacc ctaaagctaa attagataaa ttaatggaat ttctacctgg
     4801 tccagatttt ccaactggcg ctatcattca aggaaaagat gaaattcgta aggcatatga
     4861 gactggtaag gggagagtag cggttcgctc gcgaactgct attgaaacct taaaaggtgg
     4921 taagaaacaa attattgtta ctgaaattcc ttatgaagtt aataagtcag ttttagtaaa
     4981 acgaatcgat gatgttcgtg ttaataataa agttccagga atagcagagg tgcgtgatga
     5041 gtctgataga gatggtcttc gtatcgctat tgagctcaaa aaagaagccg atgaaacaat
     5101 tgtcttaaat tatcttttta aatatacaga cctacaagtg aattataatt tcaatatggt
     5161 tgctattgat gactacaccc ctaagcaggt gggtctatca agaatattaa ctagctatat
     5221 cgctcaccga agagaaatta ttattgctcg ttcaaaattt gataaagaaa aagccgaaaa
     5281 acgtctccat attgttgagg gactgattag ggttctttct attttggatg aagtgattgc
     5341 attaattcgt gcttctgaga ataaagctga tgcgaaagag aatttgaagg tcagctatga
     5401 gttttcagaa gcccaagcag aggctatcgt cacattgcaa ttatatcgtc tcacaaatac
     5461 tgatattgtg acgttgagag aagaagaaga agaacttcgt caacaaatca caatgcttaa
     5521 ggcaattatt agtgatgaac gtacgatgta taacgtaatg aaacgtgagt tacgtgaggt
     5581 caaaaagaaa tttgccaata ctagacgttc tgaacttcaa gaattagctg aaactattga
     5641 aatcgatact gcaagtttaa ttatcgaaga ggacacatac gtcagtgtta ctagaggcgg
     5701 atatgtaaaa cgcaccagtc ctcgctcttt caatgcatca actgttgatg aacttggtaa
     5761 gcgagaagat gacgaattga tatttgtgag caatgcaaag actacccaac atttattgat
     5821 gtttactaac cttggtaatc ttgcttatcg tccagttcat gaattagctg atattcgatg
     5881 gaaagacgtt ggtgaacatt tatcacaaaa tcttgttaac tttgcttcaa atgaagaaat
     5941 tatttatgct gaattagtag atgattttac taaagaaact tattttgctg tcactagctt
     6001 agggcaaata aagcgttttg aacgtcaaga aatcagtcca tggcggacat ataagtcaaa
     6061 aacagctaaa tatgctaagt taaaaaatgt agatgattac gttgtgactg ttgctcctat
     6121 ccaattagag gatgttatac ttgtcactta taatggttat gcgttgagat ttagtataaa
     6181 tgatgtacca gtcgttggta gtaaagccgc aggtgtaaaa gcgatgaatt taaaagatag
     6241 tgatcacatc gtatctgcat ttatagctaa caccacaagt ttatatttat taactcatag
     6301 aggaagtctt aaacgaatgg ccattgatgt tatccccaca actagtcgtg ctaatcgagg
     6361 acttcaagtt cttcgtgaat tgaaatccaa accgcatcgc gtttttaaag caggacctgt
     6421 atatttagag gattcatctt ttgaatttga tttatttagt tcagtatcga atcatgaagg
     6481 agatactttc gtacttgaga ttatgtctaa aacaggaaaa gtttacgatg ttgatttaag
     6541 tcaatggagt ttttctgaac gtacaagtaa tggctctttt gtatctgata agatttctga
     6601 cgaagaagta ttctcagtta aaattaagta aaacacattt gtgtttttac tagtttggta
     6661 atttatctga attttacaaa taatattgaa aaaataatga taatagcgta caatagttag
     6721 aaacccaaag aaagaggtac cttatgacag taaatttaga ttgggataat ttaggttttg
     6781 cttacagaaa attaccattc cgatacattt cgcattttaa agatgggaaa tgggatgatg
     6841 gcaagttgac agatgatgca acgctacata tttcagaaag ttctcctgcc ctccactatg
     6901 ggcaacaagc ttttgaagga ttgaaagctt atagaactaa agatggctct attcagttat
     6961 ttcgtccaga tcaaaatgca gaacgacttc aaagaacagc agatcgtctt ttaatgccac
     7021 atgttccgac agacaaattt attgcagcgg ttaaatctgt tgtacgcgct aatgaggagt
     7081 ttgttccacc gtatggtaca ggggcaacat tatacattcg tcccttatta attggtgtgg
     7141 gagatattat tggagttaaa cctgctgaag aatatatttt tacggtattt gcaatgccag
     7201 taggtagtta ttttaaaggt ggtttaacac caactaactt tattgtttcc aaagaatatg
     7261 atagagcagc accgaatggt acaggagctg caaaagttgg tggtaactat gctgctagtc
     7321 ttcttcctgg gaaatacgct catgagaaac aattttcaga tgttatctat cttgatccag
     7381 cgacacatac taaaattgaa gaggttggag cagctaattt ctttggaatt actaaagata
     7441 atcagtttat cacccctttg agtccttcaa ttttaccttc aattactaaa tattcattgc
     7501 tttaccttgc taaagaaagg tttggtatgg aagcgattga aggagatgtc tttgtcgacg
     7561 aattagataa atttacagaa gctggcgcat gtggtacagc cgcagttatt tcaccaattg
     7621 gaggaattca aaacggagat gacttccatg tcttctacag tgagacagaa gttggtccag
     7681 cgacacgtaa actatatgat gagttagtag ggatccagtt tggtgatgtt gaagcaccag
     7741 aagggtggat ttataaagta gattaagtct atactaaaag cttgctcttg caagctttta
     7801 gtattttgtg tacaataaaa aacaataaaa gagatattgg agttaatagt gagtaaaaaa
     7861 gataagaaaa ttgaaattca aatttcagac gctcaagtaa cagttaatgg aactaaggtt
     7921 gatggatacc aactcgttat tggaaaaaaa attgtaggtc aaattgcaga gctagattct
     7981 aaatttgctg tgattaaagg tgagcaagtg agtggattct ataaaaattt agatcaagct
     8041 atgggaaata ttattgaaga atataatctt aatcgctaat atttatttaa aaagcttgcg
     8101 tgctaacaac atctgtggta gaatagtcag tgttgttacg gaaagatagc gaagaggcta
     8161 aacgcggcgg actgtaaatc cgctccttcg ggttcggggg ttcgaatccc tctctttcca
     8221 ttatttgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
     8281 gaatccagct accccagtca aaggtatcaa acttttgttt gacaccgtca aagagatatt
     8341 tgttgtcctt gcgggttacc ttgagaaaat atattgttat caaaagctag ctcggctagc
     8401 ttttgttgga ggatttttta gaatgaatga atttgaagat ttgctaaaca gtgttagcga
     8461 agtgaatcct ggtgacgttg tcactgcgga agttttaaca gttgataacg gtcaagcaaa
     8521 cgttgttatc gatggtactg gtgttgaagg tgtcttgaca cttcgtgaat tgactaacga
     8581 tcgtgatgct gatatcaatg attttgttaa agctggcgac acagttgaag tacttgttct
     8641 tcgtcaagtt gtaggtaaag atactgatac agtaactttc cttgtatcta aaaaacgctt
     8701 ggaagctcgt aaagcatggg acaaacttgt tggtcgtgaa ggtgaagttg ttactgttaa
     8761 aggtacacgt gctgttaaag gcggactttc agttgaattt gaaggacttc gtggtttcat
     8821 cccagcttca atgattgata ctcgttttgt acgtaacact gaaaaatttg ttggacaaga
     8881 atttgatgct aaaatcaaag aagttgatgc agcagaaaat cgcttcatcc tttcacgtcg
     8941 tgaagttgtt gaagaatcag cagcagctgc acgtaaagaa gttttctcaa acattgaagt
     9001 aggttcagtt gtaactggta aagttgctcg tttaacaagc tttggtgctt tcattgacct
     9061 tggtggtgtt gatggacttg tacacgtaac tgaattatca catgagcgca atgtttcacc
     9121 taaatcagtt gtaactgttg gtgaagaagt tgaagttaaa gtactttcaa ttgatgaaga
     9181 agctggtcgt gtatcacttt cacttaaagc aactacacct ggcccatggg atggtgttga
     9241 acaaaaactt gctgctggtg atgttattga aggtaaagta aaacgcttga ctgactttgg
     9301 tgcttttgtt gaagtgttac ctggcattga tggacttgta cacatttcac aaatttcaca
     9361 caaacgtgtt gaaaatccta aagacgtatt atctgcagga caagaagtta ctgttaaagt
     9421 tcttgaagtt aattcagatg cagaacgtgt atcactttca atgaaagctc ttgaagagcg
     9481 tccagcgcaa gcagaaggtg aaaaagaaga aaaacgtcaa tcacgtccac gtcgtccacg
     9541 tcgtcaagaa aaacgcgact atgagcttcc agaaacacaa actggattct ctatggctga
     9601 cttattcggt gatattgaat tataataatt aaaagacttg ctaaagcaag tctttttgtt
     9661 tttgttgata aagtatgcta taatataaca tgtactacca tctttagtgt aatggatatc
     9721 acacaagatt ccggttcttg ggataggggt tcgattccct taagatggat aatagatgct
     9781 ccataaagct ttatgttata ggctttatat tagtttatct ccaaccttgc ctcacaaact
     9841 gattctcctc atttctgata agtaaaacta gggttgacaa ataagggaga atcagtttga
     9901 aatctgtgta agttagtatc ataattagtt agtatgacca ttaaggggct aagttaagat
     9961 actaagatta aactattgtg taataaaata tttctaataa gtaattaagg tattaataaa
    10021 aaggcgttaa tgccttttta ttttaaacca tgctgttgtt tgttagtgag tttatcattt
    10081 tcaaatgtaa ggttaattcc tggatcacta tctgacgact gaatgccact aatccatgca
    10141 gcctgtagtt cctctttatc tgaagaaact gcttgagaaa tatcatcagg ttctcctaga
    10201 agttctttta ctttattgta tgacatgcca ttttttaaac tattataatc ttttgttgtg
    10261 atgttggcat ctcgaataaa cttaaaatta ctaattgaac gaacaatgct actatcgttt
    10321 aataattgta tagtgatgga gatatcatcg acattccaac gatagccttt aagagtgaca
    10381 tttcccgctg gtgtatcaaa tttttcattt ggttcgccac caaataattg ttttaattca
    10441 gcgagacta
//
LOCUS       DATRTN010000062        10404 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.062, whole genome shotgun sequence.
ACCESSION   DATRTN010000062 DATRTN010000000
VERSION     DATRTN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10404
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.062"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            131..1093
                     /locus_tag="VBE22_001475"
     CDS             131..1093
                     /locus_tag="VBE22_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO7202920.1"
                     /translation="MKKLLTTPVMGCTLVILSLTSLFVGVKSIPLEQITHLDQSQVDI
                     FLTSRLPRTISILISGASLSVCGLLMQQLTQNKFVSPTTSGTMDWAKLGVVVTLIFFK
                     NTSIFIQLCIASGFAILGSLLFVTILKMITFKDNIFIPLIGLMLGQIVAAATVFLGTH
                     FQVLQSVNSWLQGNFSIMTSHRYEILYLALPCLFLVYFFAHQFTIVGLGESFAKNLGV
                     AYEKMIYFGLVLVSIMTSLVIIIVGALPFLGLIVPNLISITKGDHMSSTILETSLLGA
                     CIVMICDLFGRLVIFPYEVSIGVTLGVLGSAFFLISIIRNEGKS"
     gene            1090..2064
                     /locus_tag="VBE22_001476"
     CDS             1090..2064
                     /locus_tag="VBE22_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO7202921.1"
                     /translation="MIKDLEIPKLLILLILLIAAIILFLIYGIPTDANEFLIIYILKT
                     RYQKLIALILVGICIGSSSLIFQTLTNNRLLTPSIIGLDSLYILIQTGLMYLIGAQRV
                     IKFSSFSSFLLSLLLMVGFAYLLFTILFRNKKQSLYFVLLAGLIFNTLFSSISSFIQA
                     IMDPNDFMILQNQLFASFNAINTKILWISFIIIVVSFVINWPFIKELDVLLLGKENAI
                     SLGISYQKLTTRFFLWLALMVAIATALVGPITFLGLLVAHITYHSFHTFRHQILVPIA
                     IVICIFTLVLGQHLVQNLLHLTVQLSVLLNLIGGSYFIFTLIKGRKNL"
     gene            2061..2822
                     /locus_tag="VBE22_001477"
     CDS             2061..2822
                     /locus_tag="VBE22_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7202922.1"
                     /translation="MIQINNLHKFYGQKEILKDINISIPKGKVTAILGPNGSGKSTLL
                     SCISRLEPYDNGEIFLDKVPLAHYSSNDLAKTLAILRQSNHLTLKIKVRDLIGFGRFP
                     YSKGRLSQKDKAVIESVISYMDLNDIADEFINNLSGGQIQRAFIAMTMAQDTQYICLD
                     EPLNNLDMKYVVQMMDLIKRYAYEFNKTIVIIIHDINFATHYADNVVALKEGQVVTCG
                     TVEDVMQEKILSHLFDMPIRIETVDGKPIILFCTT"
     gene            2884..3912
                     /locus_tag="VBE22_001478"
     CDS             2884..3912
                     /locus_tag="VBE22_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7202923.1"
                     /translation="MTKKLIIAILALCTILTTSQAVLAKEKSQTVTIKNNYSVYIKKE
                     KRDKPDNKKQISETLKVPLKPKKVVVFDMGALDTITALGAEKSVIGIPKAKNALSLLP
                     NNVKSVYKAKRYQDVGSLFEPNFEAIARMQPDVVFLGARMASVDNIEKLKEAAPKAAL
                     VYAGVDSKKVFDKGVAERVTMLGKIFDQNKKAKTFNKDIAQAVLKLQKTIEKKGKPTA
                     LFVMANSGELLTQSPSGRFGWIFSVGGFKAVNENEKLSSHGTPVSYEYIAEKNPNYLF
                     VLDRGATIGQGASSKELFNNDVIKATDAVKNKRVHEVDGKDWYINSGGSRVTLRMIKD
                     VQNFVDNR"
     gene            4050..4892
                     /gene="dprA"
                     /locus_tag="VBE22_001479"
     CDS             4050..4892
                     /gene="dprA"
                     /locus_tag="VBE22_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEO7202924.1"
                     /translation="MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSK
                     CKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNYPLELKEIYNPPVLLFYQGNIEL
                     LSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARGIDTAAHVSALKNGGSS
                     IAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFTGEQPLKFHFPERNRIIAGLCQGI
                     VVAEAKMRSGSLITCERALEEGREVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDIL
                     SEYQ"
     gene            4987..7107
                     /gene="topA"
                     /locus_tag="VBE22_001480"
     CDS             4987..7107
                     /gene="topA"
                     /locus_tag="VBE22_001480"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEO7202925.1"
                     /translation="MATTTKTSTKKTSKKKSATAKKNLVIVESPAKAKTIEKYLGRNY
                     KVVASVGHIRDLKKSSMSIDFENNYEPQYINIRGKGPLINDLKKEAKKAKKVYLASDP
                     DREGEAISWHLAHILDLDKEDRNRVVFNEITKDAVKNAFVEPRQINMDLVDAQQARRV
                     LDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENEIKAFQPEEYWTIDGSFKK
                     GTRKFNATFYGLDGKKFKLSNNEDVKTVLKRIKTDEFLVEKVEKKERRRNAPLPYTTS
                     SLQQDAANKINFRTRKTMMIAQQLYEGLSLGTAGHQGLITYMRTDSTRISPLAQNEAT
                     EFITNRFGANYSKHGNKVKNASGAQDAHEAIRPSSVNHTPESIAKYLDKDQLKLYTLI
                     WNRFIASQMTAAVFDTMKVNLTQNGVTFIANGSQVKFDGYMAVYNDTDKNKMLPDMEE
                     GESVKKVNTNPEQHFTQPPARFSEASLIKTLEENGVGRPSTYAPTLETIQKRYYVKLA
                     AKRFEPTELGEIVNSLIVEFFPDIVDVTFTAEMEGKLDEVEIGKEQWQKIIDEFYKPF
                     EKELAKAETEMEKIQIKDEPAGFDCELCGSPMVIKLGRYGKFYACSNFPECHNTKAIT
                     KEIGVICPICQKGQVIERKTKRNRIFYGCDRYPECEFTSWDKPIGRTCPKSNDFLVEK
                     KVRGGGKQVVCSNEKCDYQEEKIK"
     gene            7244..7945
                     /locus_tag="VBE22_001481"
     CDS             7244..7945
                     /locus_tag="VBE22_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202926.1"
                     /translation="MSYIEMKHSYKRYQTGETEIVANNDISFSIERGELVVILGASGA
                     GKSTVLNILGGMDSNSEGEVLIDGKNIANYTIRELTRYRRYDVGFVFQFYNLVPNLTA
                     LENVELASEIVPKALDAQQALENVGLGHRINHFPAQLSGGEQQRVAIARAIAKKPKLL
                     LCDEPTGALDYQTGKQVLAILQKMAQSKETTVIIVTHNTALAPIANRVIHMHDSKISD
                     IVINENPSDIQNIEY"
     gene            7957..>10404
                     /locus_tag="VBE22_001482"
     CDS             7957..>10404
                     /locus_tag="VBE22_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7202927.1"
                     /translation="MGKTFWKDIYRSITTSKGRFSSILLLMMLGSFAFIGLKVSAPNM
                     QRTAQNYLAHHHVMDITVFNSWGLDKHDQTVLESLKGSQVEFSYFVDTTPQQNSKSYR
                     LYSNTKTISTFDLVKGRLPLNKSEIALSFQERKKYAIGDKINFKQDKNKLFSNTGPLT
                     IVGFVNSTEIWSKTNLGSSQTGDGDLDSYGVLDKTAFHSPVYTMARITFKDLRLINPF
                     SISYKEKVAKYQEKVSRKLNIHNKIRYTKTKKESLRKIDEEEKSLLKAQKQINRLDND
                     SLAMPLSQRQAIQMKIKQDRLSLLKRTKELLKLRHNTQIMESPQIIVYNRTTFPGGQG
                     YNTFDSSTNSTSKISNLFPIILYLVAALVTLTTMTRFVEEERTNAGILKALGYSDRQV
                     IFKFIIYGFIAGTLGTTLGIIGGHYLLPRIISDIISKDLTIPNTQYHLFLNYSLLAFV
                     FSLLSIVLPVFVITRRELKEKAAFLLLPKPPAKGSKIALEYINWIWKKLSFTQKVTAR
                     NIFRYKQRMIMTIFGVAGSVALLFSGLGIQSSLKQTVNEHFGRIMPYDILLTYNTNAS
                     PPKILELLSKDSKIDKYQPIHLENLDESIPGQINKQSISLFITDKKQLLPFIYLQEAT
                     TNKSLHLNNKGIIISKKLAQFYHVNTGDFIHLSHSQTLPSRKLKITGVVNANVGHYIF
                     MTKQYYRTIFKKEAKDNAFLVKLTKHKIANNLAEKLLEINGVESLTQNALQLASVEAV
                     VRSLDGSMTILVVVSLLLAIVILYNLTNINLAERKRELSTIKVLGFYNEEVTLYIYRE
                     TIILSTIGVILGTISGTY"
BASE COUNT         3558 a         1737 c         1788 g         3321 t
ORIGIN      
        1 aaaaaccttg atataataat gttttcttaa gtataaaatt ctttaaaaag tagatatttt
       61 tccaaaaaat tatgttatta tagatttgtg ctcatttttg aacactttat ctttcatttt
      121 aaggagtttt atgaaaaagc tattaaccac tcctgtaatg ggttgtactt tggttatact
      181 ttctctaacg tccctatttg ttggtgtgaa atctatccca ctggagcaaa taacacacct
      241 tgatcaatca caggtagata tttttttaac gagtcgctta ccaagaacca ttagtatcct
      301 aatatctggt gcaagtttgt cagtctgtgg cctgctgatg caacaactaa cccaaaacaa
      361 gttcgtttca ccgacaactt cagggactat ggattgggcg aaattaggag tagttgttac
      421 gctgattttc tttaaaaata cctctatttt tattcaacta tgtatagctt caggttttgc
      481 cattttagga tcgcttcttt ttgtaaccat tttaaaaatg attactttta aggataatat
      541 ttttatacct cttatcggac tgatgcttgg acaaattgta gcagcagcaa cagtattttt
      601 aggaacacat tttcaagtac tacaatctgt caacagttgg cttcaaggta atttttccat
      661 catgactagc catcgctacg aaattcttta cttagcattg ccatgtttgt ttttagtata
      721 tttttttgct catcagttta ccattgttgg gcttggagaa agcttcgcta agaatttagg
      781 ggttgcttat gaaaagatga tttactttgg tttagttttg gtttcaatta tgacatccct
      841 agtcattatt atcgtaggag ctttaccttt cttaggttta atcgtaccta atctaattag
      901 cataactaaa ggagatcata tgtcctcaac tatcttagag acatcgcttt taggggcttg
      961 tatcgtcatg atatgtgatc tttttggtcg cttagttatt tttccttatg aagtctcaat
     1021 aggagttacg ctaggagtat taggtagtgc ctttttcctg atttcaatta taagaaatga
     1081 gggaaaatca tgattaagga tttagagata ccaaaattac ttattttact gatactacta
     1141 attgctgcta ttattttgtt tttaatttat ggtattccaa ctgacgcaaa tgaatttttg
     1201 attatctata tcctaaaaac acgttatcaa aaactgattg cattaatttt ggttggtatc
     1261 tgcattggta gttctagtct tatttttcaa actctaacca ataaccgact tttaactccg
     1321 tccattattg gtttagatag cttatatatc cttatccaaa caggattaat gtatttaatc
     1381 ggagctcagc gtgtcataaa attttcatct tttagtagct tcctcctttc attattatta
     1441 atggttggat ttgcttactt attatttacg atactttttc gaaataaaaa acagtctttg
     1501 tattttgttc tattagctgg actcatattt aatacattat tttcttctat ctcatccttt
     1561 attcaagcca ttatggaccc caacgatttt atgattttac aaaatcagct ctttgctagt
     1621 ttcaatgcta ttaacaccaa gatcctttgg atttctttca ttatcattgt cgttagcttt
     1681 gtcattaatt ggccttttat aaaggaattg gatgtcttac tattgggtaa agaaaatgcg
     1741 atatcactcg gcatctcata tcaaaagctt actactagat tttttttatg gttagcattg
     1801 atggtagcta tagcaacagc attagttggg ccgattactt ttttaggctt acttgtagca
     1861 catattacct atcacagttt tcataccttt agacatcaaa tattagttcc tatagccatt
     1921 gttatctgta tctttacact tgtcttaggg cagcatctcg ttcaaaatct attacacctc
     1981 actgttcaac taagtgttct cctcaacctt atcgggggaa gttattttat attcacctta
     2041 atcaaaggaa ggaaaaactt atgattcaaa taaacaattt acataagttc tatggccaaa
     2101 aggaaatatt aaaagacatt aatatttcga ttcctaaagg aaaagtaaca gctattttag
     2161 gtccaaatgg atctggaaag agtacattgc tgagttgtat cagtcgactt gaaccttatg
     2221 ataatggtga aattttcctt gataaagtac cattagccca ctacagttca aatgacttag
     2281 ccaaaacatt agccatatta agacaatcta atcacttaac actcaagata aaggtgagag
     2341 acttgatagg attcggtaga tttccttata gtaaaggacg gttaagtcaa aaagataaag
     2401 ctgtcataga atcagttata tcttatatgg atttaaacga tatagctgac gagttcatta
     2461 ataacctcag cggaggtcaa attcaaagag cttttatcgc aatgacaatg gcacaagata
     2521 ctcaatatat ttgcttagat gagccactca acaatttaga tatgaaatat gttgttcaaa
     2581 tgatggatct tatcaaacga tatgcgtatg agtttaataa aactatagtg attattattc
     2641 acgacatcaa ttttgcaact cactacgctg acaatgttgt cgctttaaaa gaaggacaag
     2701 tggtgacttg tgggactgtc gaagatgtta tgcaagaaaa aatcctttct catttatttg
     2761 atatgcctat ccgtatcgaa actgttgatg gtaaaccaat tatccttttt tgtacaacgt
     2821 aaataaagag atttatgtag gataacattt ttagttttta tctattttag gagattttat
     2881 ttaatgacaa aaaaacttat tattgctata ttagcactat gcactatctt aaccacttct
     2941 caagctgttt tagctaaaga aaaatcacaa actgttacca taaaaaacaa ctattcggtc
     3001 tatattaaaa aagaaaaaag agacaagccg gataataaaa agcaaatcag cgagacactt
     3061 aaagttcctt taaaacccaa aaaagtagtt gtttttgata tgggagcttt ggatactatc
     3121 acagctttag gagctgaaaa atctgttatt ggtatcccga aggctaaaaa tgctctaagt
     3181 ttattgccca ataacgtcaa atctgtttat aaagctaaga gataccaaga cgtaggaagt
     3241 ctcttcgaac caaactttga agctattgct cgtatgcaac ctgatgtggt tttcctagga
     3301 gcacgtatgg cttctgttga taatattgaa aaattaaagg aggctgcacc taaagcagca
     3361 ttagtatatg ctggagtcga ctcaaaaaaa gtatttgaca aaggagttgc tgagcgtgtc
     3421 acaatgttag ggaaaatctt cgaccaaaat aaaaaggcaa aaacctttaa taaagatatc
     3481 gcacaagctg ttcttaaatt gcagaaaact attgagaaaa aaggtaaacc tacagctcta
     3541 tttgtaatgg caaacagcgg tgaactttta actcaatcac cttctggtcg ttttggttgg
     3601 attttctctg taggtggatt taaagcagtc aatgaaaatg aaaaactaag ttcacatggt
     3661 actcccgtat cttatgaata catcgctgaa aaaaatccta actatctctt tgttttagat
     3721 cgtggagcga ctattggaca aggagcttca tcaaaagaac tttttaataa cgatgttatt
     3781 aaagcaactg atgctgtcaa aaacaaacgt gttcatgagg tagatggaaa agattggtat
     3841 atcaattcag gcggaagccg agtaacactc cgtatgatta aagatgtaca gaactttgtt
     3901 gataatcgtt aattttttgt caaaggctag gaaaaatcct agtcttattt ttttgaaaaa
     3961 atagctatta tcaatgcttc agagtataga ttgatcttat cactttattt tgtctattat
     4021 tttcgaatat agtcaatgga ggtaacctta tgaatcattt tgaactattt aagcttaaaa
     4081 aagctggatt aacaaatctt aatatcaaca atattatcaa ctatctcaaa aagaatagtt
     4141 taacttctct atctgttcgc aatatggccg tagtatcaaa atgtaaaaat cctactttct
     4201 ttatagaaaa ttataaacag ctagacctta aaaaacttcg acaagaattc aaaaaatttc
     4261 cagttctatc gattttggat tctaactatc ctttagagtt aaaagaaata tataatccac
     4321 cagttctact tttttatcag ggtaatattg aacttctatc taaacctaaa ttagctgtag
     4381 tgggggcaag acaggcatct cagataggtt gtcagtctgt taaaaagatt atcaaggaga
     4441 ctaacaatca atttgttatc gtaagtggtt tagcgcgtgg cattgataca gcagcacatg
     4501 ttagtgcttt aaaaaatggc ggcagtagta tagctgttat cgggagtggt ttagatgttt
     4561 attatccaac ggagaataag aaacttcaag aatatatgtc atataatcat ctcgtattat
     4621 cagaatattt taccggagaa caacccttga aatttcattt ccccgaacgt aaccgtatta
     4681 ttgcagggct gtgtcaaggt attgtggttg ccgaagctaa gatgagatct ggaagtttaa
     4741 ttacctgtga aagagcatta gaagagggac gagaagtttt tgccattccc ggaaatatta
     4801 tcgatggcaa atcagatgga tgccaccatc ttatccaaga aggagctaaa tgcattattt
     4861 caggaaaaga tatcctttct gaatatcagt agtgttttgc tttctactat aggagaagtt
     4921 ttctattgac agtttaagaa aagtgagtta ttatgctata agtttacgat atagaaagtg
     4981 atacctatgg caactacgac aaaaacgtcg accaaaaaaa caagtaaaaa aaaatcagct
     5041 actgctaaaa aaaatctagt tattgtggag tctcctgcaa aagcaaagac tatcgaaaaa
     5101 tacttaggac gtaactataa agttgtagcc tcagtaggtc atatccgtga tttaaaaaag
     5161 tcaagtatgt ccattgattt tgagaataat tacgaaccac aatatattaa tattcgtggt
     5221 aaaggacctc tcattaatga tttaaaaaaa gaagctaaaa aggctaaaaa agtttacctc
     5281 gcgagtgacc cggaccgtga aggagaagct atttcctggc atttagcgca tattttagat
     5341 ttagacaaag aagacagaaa ccgtgttgtt ttcaatgaaa tcacaaaaga tgctgttaaa
     5401 aatgcttttg tggaacctcg tcagattaac atggatctcg ttgatgctca gcaagcacga
     5461 cgtgttttag atcgcattgt tggttattct atctcaccta ttttatggaa aaaagtaaaa
     5521 aaagggctat cagctggacg tgtacaatca gttgctctta aactaatcat tgatcgtgaa
     5581 aatgagatta aggctttcca accggaggag tattggacta ttgacggttc ctttaaaaaa
     5641 ggaacacgta aattcaatgc cactttctac ggtttagacg gaaagaaatt caaattatcg
     5701 aataatgaag acgtcaaaac agttctaaaa cgtattaaaa ctgatgaatt cttagttgaa
     5761 aaagttgaaa aaaaagagcg tcgtcgtaat gcaccattac cgtatacaac ttcttcattg
     5821 caacaagatg cagctaataa aatcaatttt cgaactcgta aaaccatgat gattgcgcaa
     5881 cagctgtacg aaggacttag cttaggcaca gcaggtcatc aaggtctgat tacctatatg
     5941 cgtactgatt ctacacgtat tagtccgtta gcacaaaatg aagcaacaga atttattact
     6001 aaccgttttg gtgcaaatta ttctaaacat gggaataaag ttaaaaatgc ttctggagca
     6061 caagacgctc acgaagctat tcgcccatct agtgttaatc atacacccga aagtattgct
     6121 aagtatttag acaaagatca actaaaactt tacactctta tctggaaccg ttttattgca
     6181 agccaaatga cagcagcagt ctttgacaca atgaaagtta atttaacgca aaatggtgtt
     6241 acttttattg ctaatggtag ccaagtcaag tttgatggtt acatggctgt ttataacgat
     6301 actgacaaaa ataaaatgtt accagatatg gaggaaggag aaagtgttaa aaaggttaat
     6361 acgaatcctg aacaacactt cactcaacct cctgcaaggt tttcagaagc aagtctcatt
     6421 aaaacacttg aagaaaacgg tgtaggtcgt ccttcaactt atgccccaac gcttgagact
     6481 attcaaaaac gttattatgt taagcttgca gctaaacgct tcgaaccaac tgaacttggt
     6541 gaaattgtta atagccttat tgtagaattt ttcccagata ttgttgatgt taccttcact
     6601 gccgaaatgg aagggaaact agacgaagtt gagattggta aagagcagtg gcaaaagatt
     6661 atcgatgaat tttataaacc atttgaaaaa gaacttgcaa aagcagaaac tgaaatggag
     6721 aaaattcaaa taaaggatga acctgctgga tttgattgcg agctatgtgg atcaccaatg
     6781 gtaataaaac taggacgtta tggaaagttt tatgcgtgta gcaatttccc tgaatgtcat
     6841 aacacaaaag ctatcactaa ggaaataggt gttatttgtc ctatctgtca aaaaggacaa
     6901 gttattgagc gaaaaacaaa acgcaatcgt atcttttatg gctgtgaccg ctacccagaa
     6961 tgtgagttta catcttggga caaacctatt ggacgaactt gccccaaatc aaatgatttc
     7021 ttagtggaga aaaaagtacg cggtggtggt aaacaagttg tttgctcaaa tgaaaaatgc
     7081 gactaccaag aagagaaaat taaataaagt ggaatctgag tgtttagctc agatttttaa
     7141 aaaaattatt attttacata acttaattta tgtatttatt tttatgggag aattaataaa
     7201 aaagtgctat aatataggtg tattcatagt gtgaggtgcc gttatgtcct atattgaaat
     7261 gaagcattcc tataagcgtt atcaaacagg tgaaaccgaa attgttgcta acaatgatat
     7321 tagtttcagt attgaaagag gtgagttggt ggttattctt ggtgcatccg gcgctggcaa
     7381 atctactgtt ctcaatatac taggagggat ggatagtaat agcgaaggtg aagttttaat
     7441 tgatggtaag aatattgcta attataccat cagggaattg actcgttacc gtagatatga
     7501 tgtcgggttt gtttttcaat tctataactt agtccctaat ctaacagcac tagagaatgt
     7561 agaattggct tcagaaattg ttccaaaggc attagatgcc caacaggcat tagaaaacgt
     7621 aggtttggga caccgtatca atcattttcc agcacagctt tccggtggcg aacagcaacg
     7681 cgttgcaata gcacgagcta ttgccaaaaa acctaaactg ttactttgtg atgaacctac
     7741 tggtgcttta gattaccaaa ctggaaaaca agtactagcc atcctgcaaa agatggcgca
     7801 atcaaaagaa acaactgtga tcattgttac tcataatact gccttagcgc caattgcaaa
     7861 tcgcgtgatc catatgcatg attcaaaaat cagtgatatt gtaataaacg agaatccaag
     7921 tgatattcag aacatagaat attagaggtt atacagatgg gaaagacatt ttggaaagat
     7981 atctatcgat caattacgac ttcaaaaggt agattttcat caatcctctt acttatgatg
     8041 ttaggctcat ttgcttttat aggactgaag gtctcagcac ctaatatgca aagaacagca
     8101 caaaattacc ttgctcatca tcatgttatg gatattaccg ttttcaattc ttggggactt
     8161 gacaaacatg atcaaactgt tttagaaagc ttaaaaggat cacaagttga attttcttac
     8221 tttgtagata caacccctca acagaatagt aaatcttatc gattgtattc caataccaaa
     8281 acgatatcaa cctttgatct tgttaaggga cgtctccctt taaataaatc agaaattgct
     8341 ctctcatttc aggaacgaaa aaaatatgca ataggtgata aaattaattt taaacaagat
     8401 aaaaacaaac tgttttcaaa tacaggtcct ctaactattg taggttttgt taattctacc
     8461 gagatttggt ctaagacaaa ccttggtagt tctcaaacag gggacggcga cttagatagt
     8521 tatggcgtat tagataagac agcatttcac tctccagtat atacaatggc aaggataact
     8581 tttaaagatc ttaggttgat taaccctttc tcaataagtt acaaagaaaa agtagcgaaa
     8641 taccaagaaa aggtatcgcg aaaattaaat attcacaata aaataagata taccaaaaca
     8701 aaaaaagaga gcctacgtaa aatcgatgag gaggaaaaaa gcctactaaa agctcaaaaa
     8761 caaatcaatc ggttagataa tgatagccta gcgatgccac tatctcaaag acaagctatt
     8821 caaatgaaaa ttaaacaaga ccgactatct cttttgaaac gaacaaaaga gcttctaaaa
     8881 ttgagacata acacccaaat aatggaatcg cctcaaatta ttgtctacaa ccgtactacc
     8941 tttcctggag gacaaggata taacactttt gactccagta caaatagcac ttctaaaatc
     9001 agtaatcttt tccccattat tttatattta gtcgcagcat tagtaacctt aacaacaatg
     9061 actaggttcg tcgaggaaga aagaaccaac gcaggcatat taaaagcact aggctatagt
     9121 gaccgccaag ttatctttaa atttattatt tatggtttta tagcaggaac attagggact
     9181 actttgggta tcattggagg gcattatctt ttacctcgta ttatttctga tatcatttcc
     9241 aaagacctga ctattccaaa cacacagtat catcttttct taaattacag tttactagcc
     9301 tttgtttttt cattattaag cattgttctt ccagtttttg ttatcactcg acgtgagttg
     9361 aaagaaaaag cagcattttt attattacca aaaccaccag ctaaaggatc taaaatagct
     9421 ttagagtata tcaattggat ttggaaaaaa ctgtccttca cacaaaaagt aactgctaga
     9481 aatatttttc gttacaaaca aaggatgatt atgaccatat ttggagttgc tggatcagta
     9541 gcgctcttat tttcaggtct aggaatacaa tcctccttaa agcaaaccgt caatgaacat
     9601 ttcggtcgta ttatgcccta tgatatattg ctaacatata acacaaatgc ttctccccca
     9661 aaaatacttg aactactttc aaaagattca aaaatagaca aataccaacc aatacacctt
     9721 gaaaatcttg acgaatccat tcctggacaa attaataaac agtcaatttc cctattcata
     9781 acagacaaaa aacaattact accttttatt tatttacaag aagcaacaac taataagtca
     9841 ttgcacttga ataataaagg gattattatt tctaaaaaat tagctcaatt ttatcatgtt
     9901 aataccggtg attttatcca tttatctcat tcacaaacac ttccttctag aaaattaaaa
     9961 ataacaggag ttgtcaatgc gaatgttggt cactatattt ttatgacaaa acaatactat
    10021 cgaactattt ttaagaaaga agctaaagat aacgcttttt tggttaagtt aaccaaacat
    10081 aaaatcgcaa ataacttagc agaaaaactt ttagaaatta atggagttga gtctcttacg
    10141 caaaatgctc ttcagctggc tagtgtagaa gccgttgtgc gttctttaga tggctctatg
    10201 actattttag ttgtcgtatc tttattgtta gccattgtaa ttctctataa tctcactaat
    10261 attaatttag cagaacgtaa gcgggagcta tcaactataa aggtattagg tttttataat
    10321 gaagaagtaa ctttatacat ttaccgcgaa accattattt tatcaaccat cggtgtgatt
    10381 ctaggcacca ttagcggtac ttat
//
LOCUS       DATRTN010000063         9998 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.063, whole genome shotgun sequence.
ACCESSION   DATRTN010000063 DATRTN010000000
VERSION     DATRTN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9998
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.063"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(27..806)
                     /locus_tag="VBE22_001483"
     CDS             complement(27..806)
                     /locus_tag="VBE22_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7202928.1"
                     /translation="MSKVKVLSLITVSGLFLMAGNLSASADVVTSGGDTIMLSGVDAG
                     VSDSITPPPSNINPVTDTIEPSAPTPSTDPITDTIEPSAPTPSTDPITDTTAPSAPTP
                     STDQTTGTTDSSTSAPSTDQTTDTTDSSTSAPSTEAEKPAPSKEPDAPKPITKPIVDV
                     PISTVTGDTVIGTENGKVIVQSASGTILKDAKEVGGEVQKDGTVAIKKSDGKIEVLPN
                     TGEGKTIFTIVGLLLIAGAVWIGFKDNIKKYLTFFKKKSEK"
     gene            complement(823..1371)
                     /locus_tag="VBE22_001484"
     CDS             complement(823..1371)
                     /locus_tag="VBE22_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202929.1"
                     /translation="MKRRKIVLFLMVLVMAFIVYKVIQKDNPVIKTSGNHTKVERVEP
                     KHKVKSYDSTKETKSTLEASDRQVSDKEKEKVKRSITVPIDYLVKIPTQSDIKGDYQN
                     KLSITNPTVAQTVKTMLMAGYHFDLESLQVYESDASNVYQFTINLVTDKRDKLSLAGN
                     YVLGTDQFEIASVHGTPVNVVF"
     gene            complement(1371..2744)
                     /locus_tag="VBE22_001485"
     CDS             complement(1371..2744)
                     /locus_tag="VBE22_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7202930.1"
                     /translation="MGILIVFPFLLVLLLTASSSSQQDCQITPDVSSQVETSSENASN
                     SDWTKKGTVANQTAEKVFNAWVSKGLSGASASGIVGWVNSEGGFAMIGRAEGHYGNDL
                     KTNSIAFNVRPTGLSYYTTEAGGGIYQITPFTKYAPLGDPKWEDADAMNAFVMKSIKS
                     GDWNKAMDLSGGNHSFEEMAQMTDPKQATLVWNAYERGNTAYINKAQKTADGQKAYEI
                     FNGSKYKFDAEKFSKSFGGGSKTSSSGKSDIISDSTVLSLCDSASNAVQGLFGKDKSG
                     KVKYTSYNAWKPDQLPSDLKKYAIDPKSVGLFYRTSSGWNAIASTGGQCTDLSASLMY
                     GLWLKGGMHPTQRMGNGNMVVSNWVSAFGGRSENTPSSGAVFSSSGTSSAGHTGVVSH
                     VFENGDMLIVEQNYSTYSGDNRGFGPYSWNYRYVSTLELKKEHYSFYNPSKAGYKLSD
                     KVKTVGS"
     gene            2907..3050
                     /locus_tag="VBE22_001486"
     CDS             2907..3050
                     /locus_tag="VBE22_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEO7202931.1"
                     /translation="MGNSSKSNRKEELDLSTFEVLTLMFIAGNFVIALVKLVLELVKS
                     TKK"
     gene            complement(3240..5240)
                     /locus_tag="VBE22_001487"
     CDS             complement(3240..5240)
                     /locus_tag="VBE22_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202932.1"
                     /translation="MDKIDDGLIASTFAKVKDVDIFALKAYMEITHGAETGAQSILLD
                     VFVNFPFFLLNLIVGLFSVILRFFENFSLYDTYKQTVYHSSQKLWENLSGNGSYTSSL
                     LYLLVAISAFSIFISYLFSKGDFSKRLIHLFVVIILGMGYFGTIQSTSGGIYILDTVH
                     QLAGSFSDAVTNLSLDNPSGGKTKITQKSSVADNYVMKTSYTAYLFVNTGQLNGKFHN
                     NQTGKEEKFDNEQVLGKYDKSGKFITPKQKDILNYTDNLGDKATEGEEKNRWLSAVND
                     YLWIKSGYVILKIFEAVILAVPLILIQLIAFMADVLVIILMFIFPLALLVSFLPRMQD
                     IIFNVLKVMFGAVSFPALAGFLTLIIFYTQTLIATFVKKKFTDGSLLSGSNFKGQAIL
                     FMLLITVFVQGCVFWGIWKYKETFLRLIIGSRASQVINQSADRINEKANDLGITPKSI
                     YERAHDMSSLAMMGAGYGVGTMMNAQDNWNAFKERQQANLDDGQSKTNDADKYDEANA
                     DDTVISKEAELTNEGEYQSELPKEASKRIEQLGKESSYELSFISEGNSTEEILKNVKS
                     DNHTFQEGDGDTSLTNQDMITNDIENHSNNYTSPLKQRKLNKLEGELSQFNSDVSMTK
                     NHGKNAFEKGFNASKTKEVRKQHNLERQSKVLEELEKLREGK"
     gene            complement(5256..7757)
                     /locus_tag="VBE22_001488"
     CDS             complement(5256..7757)
                     /locus_tag="VBE22_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7202933.1"
                     /translation="MVKKLEYPIDRIHQNLALRKDKVVVAYYRIPNTPITITDSDKKD
                     SHKAKVAQVIKKLAKYKNFDISLIPKDYLLGEKMSDFEADLADDVKELGLETLDFTVD
                     TLTHEMEIPYQFDWLIGVDLGKGQYNANIKEFIYNQFESIASNFASLAGYEVEVDEDW
                     YKEHSEEELLVYSLLSTLKAKRLTDVDLFYYQRMQFLRYVPHTKSEVIANRNMLNVTD
                     TLIKSLEGGFLKLESAYGSSFVSVLPVGRFSTIFNGFHLGELVQRMSFPVELRFQAEF
                     IDKTKLGGTMGRSNTRYDQIMKEAYNTNTVQQDDILMGAYSLKDLMKKVGNKEEIIEY
                     GCYLVVAGSSLNQLKQRRYAILSYFDDMKVNVYEASHDTPYLFQALLYGQDLQKTTRK
                     WNHLVTARGFSELMLFTNTQSGNRIGWYIGRVDNRLTAWDSIDEAIMGSKNLVLFNAT
                     VANKEDVAGKVTKNPHVIITGATGQGKSYLAQMIFLHTAQQNVRVLYIDPKRELRQHY
                     LKVVSDPEYARKFPLRKKQIEETNFVTLDSSVKENHGVLDPIVILDKEGASSTAKNML
                     LYLLKNATEIKLDQTTALTEAISQVIAKREAGEVVGFNQVIEALIDSESDEVQSVGRY
                     FKAIIQNSILELAFSDGDVAGLSYEERVTVLEVADLSLPKDGSDHISDHESNSIALMF
                     ALGAFCKHFGERSDDETVEIFDEAWVLMQSSEGKAVIKSMRRVGRSKYNVLMLVSQSV
                     HDAENDDDTTGFGTIFSFYEKSEREDILKHVGLEVTPKNLEWIDNMISGQCLYYDVYG
                     NLNMISIHNIHPDIDPLLKPMKKTVSSHLENKYAS"
     gene            complement(7778..8188)
                     /locus_tag="VBE22_001489"
     CDS             complement(7778..8188)
                     /locus_tag="VBE22_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7202934.1"
                     /translation="MEEEKYFDYSRGLNAPYWIQEVKTAKGKVIWYFSTPMQLSFFIV
                     FFIILVLMLTVCSPLMQVLNALTHSVSMLLYWFVPSKLAKFYVEYEPQGKKMHIFLWD
                     YLVYLKDFGLNKKGIYQGERVDIVEEIVFEKTKI"
     gene            complement(8192..8458)
                     /locus_tag="VBE22_001490"
     CDS             complement(8192..8458)
                     /locus_tag="VBE22_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202935.1"
                     /translation="MNLVKVNSVILAGGPNVSGLKQFFQGDGIYLISVIAVFIIIKNW
                     KEANWLKVFSTLAIYAIIVSMLKGQQILSFLGGMLRWFGVETGL"
     gene            complement(8479..9432)
                     /locus_tag="VBE22_001491"
     CDS             complement(8479..9432)
                     /locus_tag="VBE22_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEO7202936.1"
                     /translation="MIDIQKIKKFLLRYTKKEEGGKQPKTKEVKQRTANFIVYGILGL
                     LFIVGFFGSLRAIGLSNQVQHLKETVIAVEKKSKHKKTDDSLDISRIQYYMNNFVYYY
                     INYSQDTADQRKTELENYYSFSTASMTDDVRKSRTLQTQRLISVEKEKDYYIALMRIG
                     YEVDKKSYQMNLAVPFQMQRGLLAIVSQPYTVAEDLYLGKSKAFEKKTLDQVKELSKE
                     QVSSIQKFLPVFFNKYALSNKTDLKLLMKTPELMGKGFKVSELDLNNAIYYQEKKHQV
                     VQLSVTFEDLVTGGTRSENFTLYLSKADNGWYVEEMYHYFK"
     gene            complement(9485..9739)
                     /locus_tag="VBE22_001492"
     CDS             complement(9485..9739)
                     /locus_tag="VBE22_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002279547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEO7202937.1"
                     /translation="MFNFTEEAWKDYVSWQQEDKKILKRINRLIEDIKRDPFEGIGKP
                     EPLKYHYSGAWSRRITEEHRLIYMIEDGEIYFLSFRDHYK"
     gene            complement(9732..9998)
                     /locus_tag="VBE22_001493"
     CDS             complement(9732..9998)
                     /locus_tag="VBE22_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEO7202938.1"
                     /translation="MEAIVYSHFRNNLKDYMKKVNDEFEPLIVVNKNPDENIVVLSQD
                     SWESLQETIRLMENDYLSHKVINGISQVKEKQVTKHGLIEVEDV"
BASE COUNT         3197 a         2006 c         1342 g         3453 t
ORIGIN      
        1 gccatgaaat taatcatggc attatcttat ttttcacttt tctttttgaa aaaggttaaa
       61 tactttttta tattatcttt aaaaccaatc caaactgctc cagcaattaa caagagacct
      121 actattgtaa agatagtctt accctcacca gtgtttggca atacttctat ttttccatct
      181 gattttttaa ttgctacagt accgtctttt tgtacctcac caccaacctc tttggcatcc
      241 tttagtattg ttccagatgc actttgaaca atcacttttc cattttcagt gccaattact
      301 gtatctcctg taactgttga aattggaaca tcaacaatag gttttgtaat aggcttaggc
      361 gcatctggtt ctttactagg tgctggtttt tctgcttctg tacttggtgc tgacgtactt
      421 gaatctgtcg tatctgttgt ttgatctgta cttggtgctg acgtacttga atctgtcgta
      481 cctgttgttt gatctgtact tggtgttggt gcacttggtg ctgtggtatc tgttattgga
      541 tctgtacttg gtgttggcgc acttggttct atagtatctg ttattggatc tgtacttggt
      601 gttggcgcac ttggttctat agtatctgtt actggattta tatttgatgg tggtggcgtt
      661 atagaatcag atacacctgc atctacccct gatagcatta tagtatcacc tccactagtt
      721 acaacatctg cacttgctga taaattacca gccattaaaa atagtcctga aacagtaatt
      781 aaacttaaaa cttttacttt tgacataaat tttccttctt tcttaaaata ctacatttac
      841 aggtgtacca tgtacactcg caatctcaaa ttggtctgta cctaatacat aattaccagc
      901 taaacttaat ttgtctcttt tatctgttac taaattaata gtaaactgat aaacattact
      961 cgcatctgac tcatatactt gtaaactttc caaatcaaaa tgataaccag ccatcaacat
     1021 ggtttttaca gtttgggcaa ctgtaggatt tgtaatggat aatttatttt gataatcacc
     1081 cttaatatct gattgtgttg ggatttttac aaggtaatca ataggtacag ttatagatct
     1141 tttaaccttc tccttttcct tatcagatac ttgacgatca ctagcttcca aagtagattt
     1201 tgtttcttta gttgaatcat aagacttaac cttatgtttc ggttcaactc gttcaacttt
     1261 tgtatgatta ccacttgttt taatcactgg attatctttt tgaataactt tataaactat
     1321 aaaagccatc actaacacca ttaaaaataa aactattttt ctacgtttca ttaactacct
     1381 accgttttca ctttatcaga caatttataa ccagctttag agggattata aaaactataa
     1441 tgctcttttt ttaattctaa agtagaaaca taacgataat tccatgaata tggtccaaat
     1501 cctctattat ctccagaata agttgaataa ttttgctcta ctattaacat atcgccattt
     1561 tcaaatacat gactaaccac tccagtatgc ccagcactag aagtacctga acttgaaaaa
     1621 acagctccag aagatggagt attttcagag cgtccaccga atgcagatac ccaattagaa
     1681 acaaccatat taccatttcc cattctttgc gttggatgca ttccgccttt aagccataaa
     1741 ccatacatca aacttgctga aagatctgta cattgaccac cagtagaagc tatcgcattc
     1801 catccactag acgttcgata aaataaacca actgacttag gatctattgc atactttttc
     1861 aaatcacttg gtaattgatc tggtttccat gcattataag aagtgtattt aactttccct
     1921 gacttatctt taccaaataa gccctgtacc gcattactag ctgaatcaca aagagataaa
     1981 actgtagaat cagaaattat atcgctctta ccagacgagg acgtttttga accaccacca
     2041 aaacttttag aaaatttttc tgcatcaaat ttatattttg aaccattaaa tatttcatag
     2101 gctttttgcc catccgcagt cttttgagct ttattaatat atgcagtatt tcctcgttcg
     2161 taagcattcc aaactaaagt ggcctgcttg ggatcagtca tctgtgccat ctcttcaaaa
     2221 gaatgatttc cacctgataa atccattgct ttattccagt ctcctgattt aatggatttc
     2281 ataacaaagg cattcatggc atctgcatct tcccatttgg gatcgcctaa tggggcatac
     2341 tttgtgaaag gtgtgatttg ataaatacca cctccagctt cagtcgtata gtaagataac
     2401 ccagtaggtc tcacattaaa ggcaattgaa tttgttttta aatcattacc ataatgccct
     2461 tctgctcgac ctatcatagc aaatccacct tctgaattaa cccaaccaac aatccctgat
     2521 gcactcgcac cagataagcc tttacttacc caagcattga ataccttctc tgctgtttga
     2581 tttgcaactg ttcccttttt tgtccaatca ctattggaag cattctcact tgaggtttca
     2641 acttgtgaag atacatctgg tgtaatttga caatcttgtt gagaacttga cgaagcagtc
     2701 aacaataaaa ccaataaaaa aggaaataca atcagtattc ccaaacagcc taattttttc
     2761 ttatgtttta ctttagttcc ctcctttcaa acaaaataaa aaagcaccaa caaaaattga
     2821 tactttaaac cactatgaaa tatgatataa taatgaagca gaaaagttaa gacggtggct
     2881 cttatttctg aaaggtaggt ggtcccatgg gcaattcatc aaaatccaac agaaaggagg
     2941 agctagattt gtctaccttt gaggtcttaa cactcatgtt tatagcaggt aacttcgtta
     3001 tcgctctcgt gaagttagtc cttgaactgg taaaatcgac aaaaaaataa ccgtctcaac
     3061 tttggcgagt taacggttat tttttaacgg ttattttctg ccaccgtctt aaacggttct
     3121 gctaagcagt tgttagaagc aactgctttt tatttacaat tatattatac ataatctaaa
     3181 aaatagtgtc aattaatata tagtttacta aacctctcaa tgtcataaaa ctccttgtac
     3241 tacttacctt ctctcagctt ttctagttct tccaaaactt ttgactgtcg ttcaagatta
     3301 tgttgctttc taacttcctt agttttactg gcattaaatc ccttttcaaa agcattttta
     3361 ccatgatttt ttgtcatgga tacatcacta ttaaattgac ttaattcacc ctctagctta
     3421 ttaagctttc gctgttttaa tggtgaagta tagttatttg aatgattttc tatatcattt
     3481 gttatcatat cctgatttgt taatgaagta tctccatctc cttcttgaaa cgtgtgatta
     3541 tccgacttaa cattttttaa aatttcctct gttgaattac cttctgatat aaacgatagc
     3601 tcataagaag attcctttcc caattgttca atccttttac ttgcttcttt aggtaactct
     3661 gattgatatt caccttcatt agtcagctca gcctctttag aaataacagt atcatccgca
     3721 ttagcttcat catacttatc agcatcatta gttttagact gaccatcatc taaattagct
     3781 tgttgtcttt ctttgaaagc attccaatta tcttgtgcat tcatcattgt accaacacca
     3841 tagccagctc ccatcatggc aaggcttgac atatcgtgtg ccctctcata aatggatttt
     3901 ggagtaatac ccaaatcatt tgctttttca tttattctat ctgcagattg attaataact
     3961 tgagaagctc tagaccctat aatcagtctt aagaaagttt ctttatattt ccaaattccc
     4021 caaaacacac aaccttggac gaaaactgtt attagcaaca taaacagtat tgcctgacct
     4081 ttaaaattac taccactaag cagggaacca tctgtaaatt ttttcttaac aaaggtggct
     4141 attaaagttt gagtatagaa gattatcaaa gttaaaaaac cagctaaggc aggaaaggaa
     4201 acagccccaa acatgacttt caaaacattg aaaatgatat cttgcattct aggtaaaaat
     4261 gaaactaata aagctaatgg aaaaataaac attaatataa tcactaaaac atctgccata
     4321 aaagcaatta actgaatgag tattaatgga actgcgagta taaccgcttc aaaaatcttt
     4381 aagataacat agcctgattt tatccaaaga taatcattta ctgcagaaag ccatctattt
     4441 ttttcctccc cttcggttgc cttatctccc aaattatcag tataattcaa tatatccttt
     4501 tgttttggag tgataaattt acctgattta tcgtacttac ctaaaacttg ttcgttatca
     4561 aacttttctt ctttacctgt ttgattattg tgaaacttac catttaactg accagtattg
     4621 acaaacaaat aagccgtata agaagttttc atcacataat tatcagctac tgatgatttt
     4681 tgtgtgatct ttgttttacc acctgatgga ttatccagtg ataaattagt taccgcatct
     4741 gaaaacgaac cagctagttg atgaacggta tctaaaatat aaataccacc tgatgttgat
     4801 tgaatcgtac caaagtaacc catacctaaa atgataacca cgaacaagtg gatcaaacgt
     4861 ttagaaaaat ctccttttga aaaaagatat gaaataaata ttgaaaaagc tgaaatcgca
     4921 accaataaat aaagcaagga gcttgtataa gacccattac ctgatagatt ttcccataat
     4981 ttttgagacg aatgataaac tgtttgttta taggtatcat acaaactgaa attttcaaaa
     5041 aagcgtagaa taacagaaaa taaaccaaca atcaagttaa gtaggaaaaa gggaaaatta
     5101 acaaaaacat ctaacaaaat gctttgagct ccagtctcag ctccgtgagt tatctccata
     5161 taagccttta acgcaaatat atccacatct tttaccttcg caaaggtcga agcaatcaaa
     5221 ccatcatcta ttttgtccat ctgattacct cccttctacg aagcatattt attttcaagg
     5281 tggctagaaa ctgttttctt cattggtttt aacaatggat caatatctgg atgaatgtta
     5341 tgtatcgaaa tcatattcaa attgccataa acatcatagt agagacattg cccagaaatc
     5401 atattatcaa tccactctaa attcttaggt gtcacctcta accctacatg ttttaagata
     5461 tcttcacgtt ccgatttttc ataaaatgaa aaaattgtcc caaagccggt cgtatcatca
     5521 tcgttttctg catcatgaac agattgagac actagcatca acacattata cttagagcga
     5581 ccaacacggc gcatagattt aataacagct tttccttctg atgactgcat gagaacccaa
     5641 gcctcatcaa aaatttcaac tgtttcatca tcactacgct caccaaaatg cttacaaaac
     5701 gcacctaaag caaacattaa agcaatcgaa ttactttcat gatcagatat atgatctgaa
     5761 ccgtccttag gcagtgataa atccgcaact tctaaaactg tcacacgttc ttcgtaagaa
     5821 agaccagcaa catcaccatc tgaaaatgcc aactctaaaa tagaattctg aataatagct
     5881 ttaaaataac gaccaactga ctgaacttca tcactttcag aatcaataag tgcctctatc
     5941 acctgattaa aaccaacgac ttcaccagct tcacgttttg caatgacttg actaattgct
     6001 tctgttaaag ctgttgtttg atctaattta atttctgtcg catttttcaa caaataaagc
     6061 aacatatttt tagccgttga tgaagcacct tccttatcaa gaataacaat aggatctaat
     6121 acaccatgat tttctttgac agaactatca agagtgacaa aattcgtttc ctcaatttgc
     6181 ttcttacgta gaggaaactt acgtgcatac tctggatcag aaacaacttt taaatagtgt
     6241 tgccgtagtt ctcgtttagg gtcaatataa aggacacgaa cattttgttg agcagtatgt
     6301 aaaaagatca tctgtgctaa gtatgatttc ccttgacctg tcgccccagt tataatcacg
     6361 tgaggatttt tagtaacctt accagcaaca tcttctttat tcgcgactgt cgcattaaat
     6421 aagacaagat ttttagatcc cataatagct tcgtcaatag aatcccaagc agttaaacga
     6481 ttatctactc gtccaatata ccaaccaatc ctgttacctg attgggtatt tgtaaaaagc
     6541 atcaactccg aaaaacctcg agcagttact aaatgattcc acttacgagt tgttttttgt
     6601 aaatcttgac cataaagtaa cgcttgaaat aaatatggtg tatcatgact ggcttcataa
     6661 acattgactt tcatatcatc aaagtaactt aaaatggcat atctacgttg ttttagttga
     6721 tttaaactag aaccagcaac taccaaataa caaccatact ctatgatttc ttccttattt
     6781 ccaacttttt tcataagatc cttaagcgaa tatgctccca tcaagatatc atcttgttga
     6841 accgtatttg tgttataagc ttctttcatg atttgatcat agcgtgtatt tgagcgcccc
     6901 atagtgccac ccaattttgt cttatctata aattccgctt gaaatcttaa ttcaaccgga
     6961 aaactcattc tttgaactag ttcaccaaga tgaaagccat taaatatggt agagaaccga
     7021 cctactggaa gaacggatac aaatgaactc ccataagcac tctccaattt taaaaaacca
     7081 ccttctagtg atttaatcaa cgtatctgtc acatttaaca tattccgatt agctataacc
     7141 tcacttttag tatgaggaac atatcttaaa aattgcatcc gttgataata aaataaatca
     7201 acatccgtta atcttttggc tttcagggta gacaataaac tatagacaag taattcttct
     7261 tctgaatgtt ctttatacca atcttcatct acttctacct cataaccagc taaagaagcg
     7321 aagtttgatg cgatagattc aaactgatta taaataaatt cttttatatt agcattgtat
     7381 tgccctttac caagatcaac tccaattaac caatcaaact gatacggtat ctccatttca
     7441 tgtgttaacg tatcaactgt aaaatcgagt gtttctaacc ctaattcttt aacatcatct
     7501 gctagatccg cttcaaaatc cgacattttt tcaccaagaa gataatcttt tggtattaat
     7561 gaaatatcaa aatttttata tttagctaat tttttgataa cctgtgctac cttcgcttta
     7621 tgactatctt ttttatcact atctgtaata gtgattggtg tatttggaat gcgatagtat
     7681 gcgacaacaa ctttatcttt tcgtaaagct aaattttgat gtattctatc aatcggatat
     7741 tccaattttt ttaccatctg atctcctttc ttagtcttta tattttcgtc ttctcaaaaa
     7801 ctatttcctc tacaatgtca acacgttctc cttgataaat ccctttttta tttaacccaa
     7861 aatctttgag ataaaccaga taatcccaca aaaagatatg catctttttt ccttgcggtt
     7921 catattctac ataaaatttc gctaacttgc ttggaacaaa ccaatacaag agcattgaaa
     7981 cggaatgcgt taaagcattt aacacctgca ttaatggaga acataccgtc agcatcaaca
     8041 ctaagatgat aaagaaaaca ataaaaaatg ataactgcat aggtgtagaa aaataccaaa
     8101 tcactttgcc ttttgccgtt ttaacttcct gaatccagta aggagcattc aagcccctgc
     8161 tgtaatcaaa atatttttct tcttccataa cttataaccc cgtctcaaca ccaaaccagc
     8221 gaagcattcc acccaaaaaa gatagaatct gttgaccttt aagcattgat acaataatcg
     8281 cataaatagc taaggttgag aaaactttta accaattagc ttctttccaa ttcttaataa
     8341 ttataaagac agcaattaca ctaatgaggt aaatcccatc accttgaaaa aattgcttta
     8401 gtcctgaaac gttgggacca cctgctaaaa taacactatt cacttttact aaattcattt
     8461 tttccctctc ttacacctct atttaaaata atgatacatc tcttcaacat accaaccatt
     8521 gtctgcttta gataaatata atgtaaagtt ttctgatcgt gtgccacctg ttacaagatc
     8581 ttcaaaagtt actgataatt gcacgacttg atgctttttt tcttgataat aaatcgcatt
     8641 attaagatct agctcactaa ctttaaatcc tttccccatc agttctggag ttttcattaa
     8701 cagtttcaag tctgtcttgt tacttaaggc atatttatta aagaaaactg gtaaaaactt
     8761 ttgaatagaa cttacttgtt ctttcgatag ttccttgact tgatctaatg ttttcttctc
     8821 aaatgcttta gatttaccta aatacaaatc ttctgccaca gtataaggtt gactaacgat
     8881 tgcaagcaat cctctttgca tctgaaaagg aacagccaag ttcatctgat atgatttttt
     8941 atcaacttca taaccaatac gcatcaaagc tatatagtaa tctttttctt tttcaacact
     9001 tattaaacgt tgtgtctgca aagtcctaga ctttctaaca tcatctgtca tactggcagt
     9061 tgagaaagaa taataatttt ctaattctgt ttttctttga tctgcagtat cttgtgaata
     9121 gttgatatag tagtagacaa aattattcat gtaatactga attctagata tatctaatga
     9181 atcgtcagtt ttcttatgtt ttgacttttt ttctacagca ataactgttt cttttaaatg
     9241 ttgtacctga ttagataagc caattgccct caatgatcca aaaaatccta caataaataa
     9301 caaacctaag ataccataga caataaaatt ggctgtcctt tgctttactt cttttgtctt
     9361 aggctgttta cctccctctt ctttctttgt atatctcaat agaaactttt ttattttttg
     9421 tatatcaatc atttaacccc tttctataaa ataaaaaagc agtcactttt tttagcgact
     9481 acttctactt ataatgatct ctaaaagata aaaaatagat ttctccatct tctatcatat
     9541 aaatcaacct atgttcttct gtaattcttc gagaccaagc ccctgaataa tggtatttta
     9601 aaggttctgg ttttccaatg ccttcaaatg ggtctctttt aatatcttct atcaaacgat
     9661 taattcgttt caagattttt ttatcttcct gttgccaaga aacataatct ttccatgctt
     9721 cttcagtaaa attaaacatc ttctacctca attaaaccat gtttagtgac ttgcttctct
     9781 ttaacttgtg atataccatt aataacctta tgtgatagat aatcattttc catcaaacga
     9841 atcgtttctt gtaaactttc ccagctatct tgagataaaa ccactatatt ttcatcagga
     9901 tttttattaa ctacaatcaa aggctcaaat tcatcattga ccttcttcat ataatctttt
     9961 aaattatttc taaaatgtga ataaacaata gcctccat
//
LOCUS       DATRTN010000064         9964 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.064, whole genome shotgun sequence.
ACCESSION   DATRTN010000064 DATRTN010000000
VERSION     DATRTN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.064"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..197
                     /locus_tag="VBE22_001494"
     CDS             <1..197
                     /locus_tag="VBE22_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7202939.1"
                     /translation="LKSKSAMYNILTFIIYYMGYKLFTFTKSITMPILLVSLGIIVVI
                     LIIGYLAVVVFAPKTFRLKS"
     gene            393..1562
                     /locus_tag="VBE22_001495"
     CDS             393..1562
                     /locus_tag="VBE22_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO7202940.1"
                     /translation="MAWLGNFFTGASFSLVMPFMVLYVEQLGAPSNKVEWYAGLSVSL
                     SALSSALVAPLWGRLADKYGRKPMMVRAGLMMTFTMGGLAFIHSVTGLLILRILNGIF
                     AGYVPNSTALIASQAPQEESGYALGTLATGVTGGMLIGPLLGGLLAEWFGIREVFLLV
                     GTILLISTLMTIFMVKEDFKPISNEETMPTTEVFKSVKSLQILIGLFVTSMIIQISAQ
                     SIAPILTLYIRHLGQTENLMFVSGLIVSGMGFSSILSSPKLGRIGDRIGNHRLLLLAL
                     LYSFLMYVLCSLAQTSLQLGVIRFLYGFGTGALMPSINSILTKIAPRQGLSRIFSYNQ
                     MFSNLGQVLGPFVGSAVSIHLGFRWVFFVTSFIVLANFVWCFINFRKYIRVKEIV"
     gene            1559..1705
                     /gene="rpmG"
                     /locus_tag="VBE22_001496"
     CDS             1559..1705
                     /gene="rpmG"
                     /locus_tag="VBE22_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO7202941.1"
                     /translation="MRVKINLKCSSCGSKNYLTSKNKSNHPDKIEVPKYCPKERKVTL
                     HIES"
     gene            1752..1988
                     /gene="secG"
                     /locus_tag="VBE22_001497"
     CDS             1752..1988
                     /gene="secG"
                     /locus_tag="VBE22_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEO7202942.1"
                     /translation="MYNLLLTILLVLSVLLIISIFMQPQKNPSSNVFDSSGSEALFER
                     SKARGFEAFMQRFTGVLVFFWLLIGLVLSILSSH"
     gene            2101..4506
                     /gene="rnr"
                     /locus_tag="VBE22_001498"
     CDS             2101..4506
                     /gene="rnr"
                     /locus_tag="VBE22_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEO7202943.1"
                     /translation="MKEKIIKYLEEHGKTSVNDLAAALDMAGAKAFPKLIKTISNLES
                     HRQLRFDDNGSLSLRKKEAKKKEITVRGLFRANKAGFGFLSIDQDEDDMFIGKNDIAY
                     AIDGDTVEAVVKKPADRLKGTAAEARVVNIVERSLKTLVGKFVLDDERPKYAGYIKSK
                     NQKINQKIYIRKEPVVLDGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVLE
                     VLESMDIVSEFPEDVIAEANAIPDAPTEKDLIGRVDLRQEVTFTIDGADAKDLDDAVH
                     IKLLDNGHFELGVHIADVSYYVTEGSALNREALSRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNLDRLTQSCIMEIDQNGKVVNHQITQSVINTTYRMTYTAVNDIIAGDEEICSEY
                     ESIVPSVQHMVTLHHTLEAMRTRRGALNFDTSEAKIMVNDKGMPVDIVIRNRGIAERM
                     IESFMLAANETVAEHYARLKLPFIYRIHEEPKAEKLQKFIDYASIFGVHIQGTATKIT
                     QSALQDFMKKVQGQPGSEVLSMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRRY
                     PDLLVHRMIRDYGDKAMDKADHFANLIPEIATQTSSLERRAIDAERIVEAMKKAEYME
                     EYVGEEFEGVVASVVKFGMFVELPNTIEGLIHVTTLPEYYHFNERTLTLQGEKSGKVF
                     RVGQQIKVKLIRSDKETGDIDFDYLPSDFDIVEKVSKSSREGRPNRSSKRELQHRISE
                     RDNKNKNTSKKKASRKPKRNSDSKSHHHKDDRTTGSTKKKTKKTFYKGVAKKGQKRKS
                     KKG"
     gene            4509..4976
                     /gene="smpB"
                     /locus_tag="VBE22_001499"
     CDS             4509..4976
                     /gene="smpB"
                     /locus_tag="VBE22_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEO7202944.1"
                     /translation="MVKGQGNVVAQNKKAHHDYTIVETIEAGIVLTGTEIKSVRAARI
                     TLKDGYAQIKNGEAWLINVHITPYDQGNIWNQDPDRTRKLLLKKREIEKISNELKGTG
                     MTLVPLKVYLKDGFAKVLLGLAKGKHDYDKRESIKRREQNRDIARQLKNYNSR"
     gene            complement(5060..6448)
                     /locus_tag="VBE22_001500"
     CDS             complement(5060..6448)
                     /locus_tag="VBE22_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO7202945.1"
                     /translation="MSKNNNDHTQKSENGMVRGLENHHVQLIAIAGTIGTGLFLGAGR
                     SISLTGPSIVLVYAITGAFMFFMMRAIGEMLYQDPNQHTFINFITKYLGPGWGFFSTL
                     SYWISLIFIGMAEITAVGEYVQFWFPEWPSWIIQIVFLAILSSINLIAVKAFGETEFW
                     FAMIKVIAILGLIATGIFMVLTNFDTGHGYHASISNITNHFEWFPKGKLNFFMAFQMV
                     FFAYLAIEFVGVTTSETANPRKVLPKAIQEIPMRIILFYAGSLLAIMAIFPWQQLPVN
                     ESPFVTVFKLAGIKWAAALINFVVLTSAASALNSTLYSTGRHLFQLANESPNTLTKAL
                     KLDQLSRQSVPSRAIIASAVIVGASALISVLPGISDAFSLITASSSGVYISIYVLIMI
                     AHWKYRKSPDFMEDGYKMPAYKILSPITLLFFLFVFVSLFLQDSTYIGAIGATIWIIG
                     FGLYSHFKHKSH"
     gene            complement(6577..7503)
                     /locus_tag="VBE22_001501"
     CDS             complement(6577..7503)
                     /locus_tag="VBE22_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7202946.1"
                     /translation="MALLSVIVPCYNEQETVSTFLTEIKKVESEMARYTHFEYIFVND
                     GSTDRTLELLKKAARQFDNVHYLSFSRHFGKDAALLAGLEHTTGDFITVMDVDLQDPP
                     TLLPEMYLKLQEGYDIVATRRKDRKGEPLIRSLFAKLFYKLINQVSDTKMVDGARDFR
                     LMTKQVVDSILELNEVNRFSKGIFSWIGYDVAYISYENRERIAGKTSWSFFNLLKYSL
                     DGFINFSEIPLAIATWIGTLSSVLSLLAIIFIIIRKLLFGDPVSGWASTVTIVLFMGG
                     IQLLSLGIIGKYISKIFLETKKRPVYIVKEEG"
     gene            complement(7632..8024)
                     /gene="gloA"
                     /locus_tag="VBE22_001502"
     CDS             complement(7632..8024)
                     /gene="gloA"
                     /locus_tag="VBE22_001502"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEO7202947.1"
                     /translation="MPFLHTCIRVKDLDASIAFYQEALGFKEVRRNDFPENQFTLVYM
                     ALEDDPSYELELTYNYDHEAYDLGNGYGHIAVGVDDLETTYDAHQKAGYSVTKISGLP
                     GKPNMFYFIQDPDGYKIEVIRLSQFKAD"
     gene            complement(8112..8786)
                     /locus_tag="VBE22_001503"
     CDS             complement(8112..8786)
                     /locus_tag="VBE22_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO7202948.1"
                     /translation="MTKEDIKKQVRRAFDFRMAIRVYNNNDIPKEDMEYILDTAWLSP
                     SSVGLEGWRFLVLDRQTIAKFRDKLKEVAWGAQYQLDTASHFVLLLAEKGAYYNADSM
                     INSLVRRGLGDPAALESRIPLYKSFQENDMKIDSERSLWDWTAKQTYIALGNMMTAAA
                     MIGVDSCPIEGFDYEKVNNILSKEGLIDDKKEAISCMVSFGYRLREPKHSRARKERQE
                     VITWVE"
     gene            9053..9895
                     /locus_tag="VBE22_001504"
     CDS             9053..9895
                     /locus_tag="VBE22_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO7202949.1"
                     /translation="METYTLSNTLNIPKIGFGTWQLTEGEEAYKAVTHALKVGYTHID
                     TAQIYGNEHSVGRAIRDSGLVRESIFLTTKIWNDKHDYHLAKASIDESLQKLGVDYID
                     LLLIHWPNPKALRENDAWKAGNAGTWKAMEEAYKEGKVKAIGVSNFMKHHLEALFETA
                     EIKPMVNQIILAPGCAQEDLVRFCKGNDILLEAYSPFGTGAIFENESIKAIAEKYGKS
                     VAQVALRWSLDNGFLPLPKSATPKNIEANLDIFDFQLNEDDITTLIQLDSGIKPKDPD
                     NVSF"
BASE COUNT         3359 a         1613 c         1916 g         3076 t
ORIGIN      
        1 gattaaaatc aaagagtgct atgtataata ttttaacctt tattatatac tatatgggat
       61 ataagctatt tacctttact aaatctataa cgatgccaat attattagta agcctaggaa
      121 ttatagttgt tattttaatt attggctatt tagcagttgt agtatttgct ccaaaaactt
      181 tccgtttaaa aagttaattt taattgtggt ataataacag aggcatgtga taacataggt
      241 ttagttacat aaaggaaatt ataatggaaa taacatctca aatagatgtt aaatctcaat
      301 aaaactgcta agatagttag tggtagaaaa gtttagaaag aagatgatgt tttatctcag
      361 ttgaaacggt taactggaag caaaatttaa aagtggcttg gctcggtaat ttttttactg
      421 gggcaagctt ttcacttgtt atgcctttta tggtattata tgtagaacaa ttaggagcac
      481 ctagcaacaa agtagagtgg tatgcaggcc tatcagtatc cttatcagcc ttgtcttcag
      541 ctttagttgc cccattatgg gggcgcttag ctgataaata tggtagaaaa ccgatgatgg
      601 taagagcagg gttgatgatg acttttacta tgggaggcct tgctttcatt catagtgtta
      661 caggacttct gattttacgt atattaaacg gcatttttgc aggctatgta cctaactcta
      721 cagctttgat agcaagtcaa gctcctcaag aggaatcagg atatgcctta ggaaccttag
      781 cgacaggagt gactggaggt atgttaattg gtccactttt agggggactt ttagcagagt
      841 ggtttggtat tagggaagtt tttttattag tgggaaccat attgttaata tctactttaa
      901 tgaccatttt tatggtaaag gaagatttca aacccatttc taacgaagaa accatgccaa
      961 ctacagaagt gttcaaaagc gttaaaagct tacaaatttt aatagggctg tttgtaacaa
     1021 gcatgattat tcaaatttct gcacaatcaa ttgcacctat tttaacactt tacattcgtc
     1081 atttaggaca gactgaaaac ttgatgtttg tttccggatt aattgtttct ggtatgggat
     1141 tctctagtat tctgagtagt ccaaagctag gaagaatcgg ggatcgcata ggcaatcatc
     1201 gtttgctatt attagctcta ctgtatagtt tcctcatgta tgttttatgt tcattggcac
     1261 agacttcatt acagttaggg gttattagat tcctgtatgg ttttggaaca ggtgccttga
     1321 tgccgagtat taattcaatc ttgactaaaa ttgctccaag acaaggttta tcaaggattt
     1381 tcagttataa ccaaatgttt agcaatttag gacaagtatt aggtcctttt gtcggttcag
     1441 cagtttctat tcatttagga ttccgatggg ttttctttgt gactagcttc atcgttttag
     1501 ctaattttgt ttggtgtttt attaatttta gaaaatatat tagagttaag gaaattgtgt
     1561 gagagttaaa attaatttaa agtgtagctc ttgtggtagt aagaattatt taaccagtaa
     1621 gaataaaagt aatcatcccg ataaaatcga ggttccaaag tattgcccaa aggagagaaa
     1681 agtaacacta catattgaat cttaataagt tttatgttag aatgtacttt gaccatagtg
     1741 gaggaattaa catgtataac ctattactca ccattctact tgttttatca gtattattga
     1801 ttatctcaat tttcatgcaa ccacaaaaga atccaagtag taatgttttt gatagtagcg
     1861 gttctgaagc cctttttgaa cgtagtaaag ctcgaggatt tgaagctttt atgcaacgtt
     1921 ttacaggtgt tttagtcttt ttctggctac taatcggctt agtattatct attttatcaa
     1981 gtcattaatg atagctaaat acagataagt gaggatgggg gatagcccaa cctcactttt
     2041 tgttagtaga atggttgtag agtaataata aagtgactaa agttactaga aaaggaaata
     2101 atgaaagaaa aaattataaa atatttggaa gaacacggta aaaccagcgt taatgactta
     2161 gctgctgccc tagatatggc aggcgcaaaa gcctttccaa aattaatcaa aacaatctca
     2221 aatttggaga gccacaggca attaagattt gacgataatg gaagtctttc tcttcgaaag
     2281 aaagaagcta agaaaaagga aatcacagta agaggtcttt ttagagcaaa taaagcagga
     2341 tttggttttc ttagtattga ccaagacgaa gatgatatgt ttatcggaaa aaacgatatt
     2401 gcttatgcta ttgatggtga cacagtagaa gctgttgtaa agaaaccagc ggaccgtctt
     2461 aaagggactg cagcagaagc gcgtgttgtt aatattgttg aacgtagttt aaagacgctt
     2521 gttggtaagt ttgtcttaga cgatgagcgc cctaaatacg caggatatat caaatcaaaa
     2581 aatcaaaaaa ttaatcaaaa aatttatata cgcaaagaac cggtcgtttt agacggaaca
     2641 gaaattatca aagtagatat tgataaatat ccgacacgag gacatgatta cttcgtagca
     2701 agtgtccgtg atatcgttgg acatcaagga gatgttggta ttgatgtttt agaagtcttg
     2761 gaaagtatgg atatcgtctc agaatttcct gaggacgtta ttgcagaagc taatgctatc
     2821 ccagatgctc caacggagaa agatttaatt gggcgtgttg atttacgtca agaggtcact
     2881 ttcactattg atggagctga cgccaaagat ttagacgatg ctgtgcatat taaattactg
     2941 gataatggtc attttgaact tggagttcat atcgcagatg tatcatacta tgttactgaa
     3001 ggctctgcct taaatcgtga agcacttagt cgtggtacgt ccgtatatgt cacagatcgc
     3061 gttgtaccaa tgttaccaga gcgtctatct aacggtatat gttcattaaa tccaaatctt
     3121 gatcgtttaa cgcagtcttg tattatggaa attgaccaaa atgggaaagt tgttaatcat
     3181 caaattacac agtcagtgat taataccact tatcgtatga cttatactgc tgtaaatgat
     3241 attattgctg gcgatgagga aatctgctca gaatatgagt ctattgtgcc ctcagtacag
     3301 catatggtta ctttacacca tactttggaa gctatgcgta cgcgtcgtgg agcgctgaat
     3361 tttgacacat ctgaagcaaa aattatggtt aatgataagg gaatgccagt tgatattgtt
     3421 attcgcaaca gaggcattgc tgaacgcatg attgagtcat ttatgttggc ggcaaatgaa
     3481 acggttgctg aacattatgc tcgtctaaaa ttaccattta tttatcgtat acacgaagag
     3541 cctaaagctg aaaaattaca aaaattcatc gattatgctt ctattttcgg tgttcacatt
     3601 caaggaacag caactaaaat cacccaatct gctttacagg actttatgaa aaaagtacaa
     3661 gggcagccag gaagtgaagt gttatctatg atgttattgc gctcgatgca acaagctaga
     3721 tattctgaac acaaccatgg acattatgga cttgctgcgg aatattacac tcactttact
     3781 agccccattc gacgttatcc agacttgtta gttcatcgta tgattcgtga ttatggtgat
     3841 aaagcgatgg ataaagctga tcattttgct aaccttatcc cagaaattgc aactcaaacg
     3901 tctagtcttg agagacgtgc cattgatgct gaacgtattg tagaggcaat gaagaaagct
     3961 gaatatatgg aagaatatgt tggtgaggaa tttgaaggtg tggttgcaag cgttgttaaa
     4021 tttggtatgt ttgtagaatt accaaatact attgaaggat tgattcacgt aacgaccttg
     4081 cctgaatatt atcactttaa tgaacgtacg ctgacgcttc aaggtgagaa atctgggaaa
     4141 gttttccgtg ttggtcaaca aattaaggtt aagttgattc gttctgataa ggaaactggt
     4201 gatatcgatt ttgattattt accatcagat tttgatattg tcgaaaaggt aagtaaaagt
     4261 tcgcgtgagg gtcgtcctaa tcgaagttct aaacgagaac ttcagcatag aatttctgag
     4321 cgtgataata agaacaaaaa tacatctaag aaaaaagcat ctcgaaaacc caaaaggaac
     4381 tcggatagca aatcacatca tcacaaagac gatagaacaa caggttcaac caagaaaaaa
     4441 acgaaaaaaa ctttctataa aggcgttgct aagaaaggtc aaaaacggaa atctaagaaa
     4501 ggttaagtat ggttaaaggt caaggaaacg ttgtagcaca aaataaaaaa gcgcatcacg
     4561 actatactat cgttgaaaca atagaagcag gaattgtttt gacaggaaca gaaatcaaaa
     4621 gtgtccgcgc agcgcgtatt acattgaaag atggatatgc acaaataaaa aatggtgaag
     4681 cgtggttgat taatgttcat attacaccat acgatcaagg taatatatgg aatcaagatc
     4741 ctgatcgtac acgaaagtta ttactaaaaa aacgtgagat tgaaaaaata tcaaatgaac
     4801 tgaaagggac tggtatgact ctggtacctc taaaagttta cttaaaagat ggttttgcta
     4861 aagttctatt gggtttggca aaaggaaaac atgattatga taagcgagag tctatcaaac
     4921 gtcgtgagca aaaccgtgat attgcccgtc agttaaaaaa ctataattca cgatgattat
     4981 caaaaggtta ggcaattgcc taaccttttt tggacgaaaa aagtgaagtt gattgaccaa
     5041 cttcactgat aatatagact taatgtgatt tatgtttgaa gtgactatat aaaccaaatc
     5101 caataatcca gatggttgct ccaatagccc cgatgtatgt tgaatcttgc aagaagagag
     5161 aaacgaatac aaagaggaag aagagaagtg tgattggtga aaggattttg taagcaggca
     5221 ttttataacc atcttccata aaatcaggtg atttacgata tttccaatgt gcaatcataa
     5281 taagaacata gatagaaatg taaacaccag aagatgatgc agtaattaag gagaaggcat
     5341 ctgaaatacc aggtaaaaca ctgataagtg ctgaagctcc aacgattaca gctgatgcaa
     5401 taatagctcg acttggaacg ctttgacgag ataattggtc taattttaac gcttttgtaa
     5461 gagtatttgg tgattcatta gccaattgga ataaatgacg ccctgttgag tataaagtag
     5521 aattaagagc agatgctgca gacgttagga caacaaagtt aatcaaagcg gcagcccact
     5581 taatcccagc tagtttgaat actgtaacga aaggtgactc attaactggt aactgttgcc
     5641 atgggaagat ggccataatt gctaataatg aacctgcgta gaataaaata atacgcatcg
     5701 gaatttcttg gattgctttt ggtaaaactt tacgtggatt agctgtttca gaagttgtta
     5761 ctccgacaaa ttcaatagct aaataagcaa agaataccat ttggaaagcc atgaagaaat
     5821 tcaatttacc tttagggaac cattcaaaat gattggtgat atttgagatt gaagcgtggt
     5881 atccgtgacc ggtgtcaaag tttgttaata ccataaagat accggttgct attaaaccaa
     5941 gaattgcaat aacttttatc atagcaaacc aaaactcggt ttctccgaat gcttttacgg
     6001 caattaagtt gatagaactt aggatagcta aaaaaacaat ttggataatc caggatggcc
     6061 attcagggaa ccaaaattga acatactctc ctacggctgt aatctctgcc ataccgataa
     6121 atattaggga aatccaataa gaaagtgttg agaaaaatcc ccaaccgggt cctaaatatt
     6181 tggtaatgaa attaataaaa gtatgctgat ttggatcttg ataaagcatc tctccaatag
     6241 cacgcatcat gaagaacatg aaagcgccag tgatagcata gacaagaact attgaaggac
     6301 ccgtcaggct aattgaacga cctgcaccaa gaaaaagccc agttccgatt gtccctgcaa
     6361 tcgcaattaa ctgtacgtga tgattttcta ggccgcgtac cataccattc tcagattttt
     6421 gagtgtggtc attattattt tttgacatta taaatactcc atctattttt gaaaaactcc
     6481 tttattatat aatttttttg caaaattcgc aataattact atacaatatt tttctatgat
     6541 aaaatctcct agctaagtga gctaagagat ttcaattcat ccctcttcct tgacaatata
     6601 gactgggcgt tttttagttt ctaggaaaat cttagagatg tatttgccta taattcctag
     6661 cgacaataac tgaataccac ccataaaaag cacgatggta actgtgcttg cccaacctga
     6721 aacgggatcc ccaaatagta attttcgaat aataataaaa atgatagcta aaagtgatag
     6781 tactgaacta agagttccaa tccaggtagc tatagctaat ggaatttctg agaagttgat
     6841 gaaaccgtct agagagtatt ttaagagatt aaagaaactc catgaggtct tacccgcaat
     6901 tcgttctcga ttctcataac tgatataagc gacatcatac ccaatccagg aaaaaatccc
     6961 ttttgagaag cgattcactt catttagctc gagaatactg tcaacaactt gttttgtcat
     7021 gagacgaaaa tcacgcgcac catcaaccat tttagtatct gacacttgat tgattaattt
     7081 ataaaaaagc ttggcaaaaa gagagcgtat caacggctcc cctttacgat ctttccgcct
     7141 tgtagcgaca atatcataac cttcttgaag ttttaggtac atttctggta ataaagtggg
     7201 tggatcttgc aagtcaacat ccataacagt aataaaatca ccagtagtat gttctaaacc
     7261 agccaataaa gcagcatctt taccaaaatg gcgtgaaaat gaaaggtaat gaacattgtc
     7321 aaattgtcta gctgctttct taagaagttc gagtgtacga tcggtagaac catcgtttac
     7381 gaaaatatat tcaaaatggg tgtaacgagc catttcggat tcaacttttt tgatttctgt
     7441 taaaaaagtt gagacagtct cttgttcatt ataacaaggg acaataactg ataataaagc
     7501 catttgtaat tctccgtatc attcttgctt aaattttaac ataatatggg aaaaaataga
     7561 aagattataa ggtaatcata aaaaggacta ggtttttgcc tcagtcctta acttaatatg
     7621 atgagatgtt attagtcagc tttaaattga ctgagtcgaa taacttcaat tttatagcca
     7681 tctggatctt gaataaagta gaacatatta ggtttacctg gtaatccaga aatttttgtt
     7741 acagagtaac cagctttctg atgggcatca taagtagttt ccaaatcgtc tacaccaaca
     7801 gcaatatgac catacccatt tcctaaatca taagcttcgt gatcatagtt ataggttaac
     7861 tctaattcgt aactagggtc gtcctctagg gccatataga ctaaagtaaa ttgattttca
     7921 ggaaagtcat tgcgacgaac ttccttaaaa ccaagtgctt cttgataaaa agcaattgag
     7981 gcgtccaaat ctttaacgcg aatacaagta tgtaaaaatg gcatagtctc tctcctattt
     8041 ctttattaaa cgtattatgt caaaataaaa ttgtcttttc aaaaatattg attataaatt
     8101 aggctgtcta tttactcaac ccaagtaatg acctcttgac gttctttcct agcacgggaa
     8161 tgtttaggtt cacgcaaacg ataaccaaat gaaaccatac aagaaatagc ttctttctta
     8221 tcgtctatta acccttcttt actaaggata ttattgacct tttcatagtc aaatccttca
     8281 attgggcatg aatctacgcc gatcatagca gcagccgtca tcatattacc aagtgcgata
     8341 taagtttgtt ttgctgtcca atcccaaaga gaacgttcag aatcaatttt catatcgttt
     8401 tcttggaaag atttgtagag aggtatccta ctctcaaggg cggctggatc acctagacca
     8461 cgtcgaacga gactgttgat catgctatca gcgttataat aagcaccttt ttcagctagt
     8521 agtaaaacaa agtgactagc agtatctagt tgatattgtg ctccccaagc aacttcctta
     8581 agtttatctc gaaattttgc aattgtttgt ctatctagta ctaaaaatcg ccagccttct
     8641 aaaccgactg aagaaggact caaccaagca gtgtctaaaa tatactccat atcttcttta
     8701 ggaatatcat tattattata aacacgaatc gccatacgaa aatcaaaagc tcttcgaact
     8761 tgttttttga tatcttcttt tgtcatttta gatcctcctg ttattaatgc tatcatatca
     8821 caatttagaa aaaatctcag caggcggtat cgtggcaaag tagagctaca aaattctaat
     8881 gactgtcaga aaaataaagg gaatgaacaa aatgataggg ttttgatgaa gaaagttata
     8941 taattttaca taaggagggg ataataccat atatcaaact atcatttaga tgtttaaaat
     9001 agatgttttt cactatgaaa ttcatttttg tcacatagaa aaggagattg ttatggaaac
     9061 atatactcta tcgaatactc ttaacattcc taaaattggt tttgggacat ggcaactaac
     9121 tgaaggtgaa gaagcttata aggcagtgac tcatgcttta aaagttggtt atactcatat
     9181 tgatactgct cagatttatg gcaacgaaca cagtgttgga cgtgctataa gagacagtgg
     9241 tttggtgcga gaaagtatct ttttgacaac taagatttgg aatgacaaac atgactatca
     9301 tttagccaaa gcttctattg atgaatctct gcagaaatta ggtgttgatt atattgatct
     9361 tcttttgatt cattggccaa atccaaaagc tcttcgtgaa aatgatgcgt ggaaagctgg
     9421 gaatgcaggc acttggaaag cgatggaaga agcttataaa gaaggaaagg taaaagctat
     9481 tggtgtttcg aatttcatga aacatcactt agaggcttta tttgaaactg ccgaaataaa
     9541 acctatggtt aatcaaatta tcctagcacc aggttgtgct caagaggatt tggttcgctt
     9601 ctgtaaagga aatgatatct tattagaagc ttacagtcca tttggaacag gtgctatttt
     9661 tgaaaatgaa tctataaaag ctattgctga aaaatatggt aaatcagttg cacaagtagc
     9721 tcttcgctgg tcacttgata atggcttttt accattacct aagtcagcga ctcccaaaaa
     9781 tattgaagca aaccttgata tttttgattt tcaattaaac gaagatgata ttactacctt
     9841 aatacaacta gattcaggta ttaaacccaa agatccagat aatgtctcat tttaataaga
     9901 aaagatggaa atgtatttcc atctttttta tgtgggatag ttccttaaag ttaagagaac
     9961 catt
//
LOCUS       DATRTN010000065         9923 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.065, whole genome shotgun sequence.
ACCESSION   DATRTN010000065 DATRTN010000000
VERSION     DATRTN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.065"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            24..458
                     /locus_tag="VBE22_001505"
     CDS             24..458
                     /locus_tag="VBE22_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO7202950.1"
                     /translation="MEYDQINSYLVDIFNRIMIIEEMSLKTSQFSDVSLKEMHTIEII
                     GKHSEVTPSDVARELMLTLGTVTTSLNKLEKKGYIERKRSSIDRRVVHLSLTKRGRLL
                     DRLHSKFHKSMVSHIIEDLGEEDIKMLTSALGNLHKFLEDLV"
     gene            458..1429
                     /locus_tag="VBE22_001506"
     CDS             458..1429
                     /locus_tag="VBE22_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEO7202951.1"
                     /translation="MVFAKISQLAHYAPSQIIKNEDLSLIMDTSDDWISSRTGIKQRH
                     ISKNETTADLANKVAEQLIEKSGYSASQIDFIIVATMTPDSMMPSTAARVQAHIGASN
                     AFAFDLSAACSGFVFALSTAEKLISSGSYQKGLVIGAETVSKVLDWTDRGTAVLFGDG
                     AGGVLLEASKEKHFLAESLNTDGSRQGLQSSQVGLNSPFSDEVLDDKFLKMDGRAIFD
                     FAIKEVSKSINHLIETSHLEKEDIDYLFLHQANRRILDKMSRKIDIARDKFPENMMDY
                     GNTSAASIPILLSESYENGLLKLDGNQTILLSGFGGGLTWGSLIVKI"
     gene            1487..1711
                     /locus_tag="VBE22_001507"
     CDS             1487..1711
                     /locus_tag="VBE22_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO7202952.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLNTTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKVK"
     gene            1865..2824
                     /gene="fabK"
                     /locus_tag="VBE22_001508"
     CDS             1865..2824
                     /gene="fabK"
                     /locus_tag="VBE22_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEO7202953.1"
                     /translation="MKTRITELLNIKYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSMTDKPFGVNIMLLSPFVDDIVDLVIEEGVKVVTTGAGNPGKY
                     MERFHEAGITVIPVVPSVALAKRMEKLGADAIITEGMEAGGHIGKLTTMTLVRQVVDA
                     VTIPVIAAGGIADGRGAAAGFMLGADAVQIGTRFVVAKESNAHPNYKAKILKAKDIDT
                     AVSAQVVGHPVRALKNKLVTTYSQAEKDYLAGRISINEIEELGAGALRNAVVDGDVIN
                     GSVMAGQIAGLIKSEETCQEILEDIYSGARQVILSEANRWSDL"
     gene            2844..3770
                     /gene="fabD"
                     /locus_tag="VBE22_001509"
     CDS             2844..3770
                     /gene="fabD"
                     /locus_tag="VBE22_001509"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEO7202954.1"
                     /translation="MNKVAFLFAGQGAQKLGMARDLYETFPIVKETFDKASHVLGYDL
                     RELIDKDLDKLNQTKYTQPAILTTSTAIYRLILKEIGLRPDMVAGLSLGEYSALVASG
                     AIRFEDAVVLVARRGQLMEAAAPAGSGKMVAVLNADRQIIEDACKKASQFGIVSPANY
                     NTPKQIVIGGESIAVNAAVEELKQQGVKRLIPLNVSGPFHTALLKPASQKLSDVLDKV
                     HFSVSEIPVIGNTEAQIMKKDDIKSLLARQVMEPVRFDESIETMKKMGMTQVVEIGPG
                     KVLSGFLKKIDSSLSVYSVEDKIGFNNLKELN"
     gene            3779..4513
                     /gene="fabG"
                     /locus_tag="VBE22_001510"
     CDS             3779..4513
                     /gene="fabG"
                     /locus_tag="VBE22_001510"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEO7202955.1"
                     /translation="MQLKDKNIFITGSSRGIGLAIAHQFAQLGANIVLNGRSEISEDL
                     IAEFADYGVKVIAISGDVSSFEDANRMIKEAIASLGSVDVLVNNAGITNDKLMLKMTV
                     EDFESVLKINLTGAFNMTQSVLKPMTKARQGAIINISSVVGLTGNVGQANYAASKAGL
                     IGFTKSVAREVAARGIRVNAIAPGFIESDMTDVIPEKMQEAILAQIPMKRIGKGKEVA
                     QVASFLAEQEYLTGQVIAIDGGMTMQ"
     gene            4529..5761
                     /gene="fabF"
                     /locus_tag="VBE22_001511"
     CDS             4529..5761
                     /gene="fabF"
                     /locus_tag="VBE22_001511"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEO7202956.1"
                     /translation="MTLQRVVVTGYGVTSPIGNTPEEFWNSLKEGNVGIGPITKFDSS
                     DFMVKNAAEIHDFPFDKYFVKKDLNRFDMYSLYALYASSEAIQHANLNLDEIDADRFG
                     VIVASGIGGIQEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMRLGAHGVCKSI
                     NTACASSNDAIGDAFRNIKFGIQDIMVVGGAEAAITKFAIAGFQSLTALSTTEDPSRA
                     SIPFDKDRNGFIMGEGSGMLVLESLGHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     LGATKAIQLALAEANIKPEEVNYVNAHGTSTPANEKGESQAVVAALGTDVPVSSTKSF
                     TGHLLGAAGAVEAIATIEAIRHSYVPMTAGTTELSEDITANVIFGQGQDADIRYAISN
                     TFGFGGHNAVLAFKRWED"
     gene            5727..6263
                     /locus_tag="VBE22_001512"
     CDS             5727..6263
                     /locus_tag="VBE22_001512"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEO7202957.1"
                     /translation="MQFWPSNAGRIKLDIQEIKDLMTQFDESSLREFSFKTSDGELSF
                     SKNEGKAPLVPTMSHMSHQPEATPTIATPVSNEAGEQTKQATEVVSEIPESTVTVAEG
                     DVVESPLVGVAYLASGPDKPNFVSVGDSVKKGQTLMIIEAMKVMNEVPAPHDGVVTEI
                     LVANEEVIEFGKGLVRIK"
     gene            6260..6682
                     /gene="fabZ"
                     /locus_tag="VBE22_001513"
     CDS             6260..6682
                     /gene="fabZ"
                     /locus_tag="VBE22_001513"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO7202958.1"
                     /translation="MIDIKEIREALPHRYPMLLVDRVLEVSEDEIVAIKNVSINEPFF
                     NGHFPEYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTAKFVKRRGTIAVVEAVAEVDGKLAASGTLTFAIGN"
     gene            6720..8090
                     /gene="accC"
                     /locus_tag="VBE22_001514"
     CDS             6720..8090
                     /gene="accC"
                     /locus_tag="VBE22_001514"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEO7202959.1"
                     /translation="MFKKILIANRGEIAVRIIRAAREMGISTVAIYSEADKESLHTIL
                     ADEAICVGPAKSAESYLNVNAILSAAIVTGAEAVHPGFGFLSENSKFATMCEEMNLKF
                     IGPSGEVMDKMGDKINARAEMIKADVPVIPGSDGQVTSVEEAVSIAEEIGYPLMLKAS
                     AGGGGKGIRKVKSADELKPAFESASQEALAAFGNGAMYIEKVIYPARHIEVQILGDSF
                     GKIVHLGERDCSLQRNNQKVLEESPSVAIGNTLRQQIGEAAVRAAEAVSYENAGTIEF
                     LLDENSGQFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIRIAAGIPLSVSQNDIKLTGH
                     AIECRINAENPQFNFAPCPGTINGLHLPAGGMGLRVDSAVYTGYTIPPYYDSMIAKVI
                     VHGENRFDALMKMQRALYELEIDGIVTNTEFQMDLISDKKVLAGDYDTSFLMEDFLPR
                     YYGNNQ"
     gene            8099..8974
                     /locus_tag="VBE22_001515"
     CDS             8099..8974
                     /locus_tag="VBE22_001515"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEO7202960.1"
                     /translation="MALFSKKDKYIRISPNKALGSSDKRSLPEVPDELFAKCPSCKHM
                     IYQKDLGLAKICPACSYNFRISAQERLLLTVDEDSFEELFTGIETKDPLNFPNYREKL
                     AATRQKTNLDEAVVTGLAKIKGQTTALAIMDSHFIMASMGTVVGEKLTRLFELATEKK
                     LPIVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNQGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQALVGFAGRRVIETTVREDLPEDFQKAEFLLEHGFVDAIINRTELRDC
                     IAQLIAFHGNEVGHE"
     gene            8967..9740
                     /locus_tag="VBE22_001516"
     CDS             8967..9740
                     /locus_tag="VBE22_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEO7202961.1"
                     /translation="MNNDVTRILKDARDQGRLTALDYAELIFDNFMELHGDRQFADDK
                     SIIGGLGYLAGRPVTIVGIQKGKNLQDNLDRHFGQPHPEGYRKALRLMKQAEKFGRPV
                     ITFINTAGAYPGVGAEERGQGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     KVWMLEHTVYSILSPEGFASILWKDGTRTTEAAQLMKMTAGELYHMEVVDKVIPEHGY
                     FSSEIVDMIKTSLISELEMLSQLSLEDLLEQRYQRFRKY"
BASE COUNT         3190 a         1484 c         2202 g         3047 t
ORIGIN      
        1 gtttaatgtg aggtggtgat atcttggaat atgatcagat taatagttat ttagttgaca
       61 tttttaatag aatcatgatt attgaggaaa tgagtttaaa gactagccaa tttagcgatg
      121 tatctttaaa agaaatgcat actattgaaa ttattgggaa acattctgag gtaactccta
      181 gcgatgtcgc tcgagaatta atgttaactt tgggaactgt aacaaccagc cttaataagt
      241 tagaaaaaaa aggctacatt gagcgtaaac ggtcaagtat tgatcgtcga gttgttcact
      301 tgagtcttac taagagaggt aggttattag atcgattgca tagtaaattt cataaatcta
      361 tggtatctca tattattgaa gacctaggtg aagaagacat aaaaatgttg acgagcgctt
      421 tagggaatct tcataaattt ttggaggact tagtctagtg gtttttgcta aaattagtca
      481 acttgcacat tatgcacctt ctcaaattat taaaaatgag gatttatcat taatcatgga
      541 taccagtgat gactggattt cgtcacgtac tggtataaaa caacgtcata ttagtaaaaa
      601 tgaaacaaca gctgatttgg caaataaggt cgcagaacaa cttattgaaa aatccggtta
      661 ttccgctagt caaattgact ttattattgt tgcaacgatg acaccagact ctatgatgcc
      721 ttctactgcc gcaagagtgc aagctcatat tggagcgagt aatgcttttg cctttgactt
      781 gagtgcagca tgtagtggtt ttgtttttgc cttgtctacc gccgaaaaat tgatatcatc
      841 aggtagctat caaaaaggtt tagtaatagg agcagaaact gtttctaaag tattagactg
      901 gactgataga gggacagcag ttttatttgg agatggagct ggaggtgtct tgctagaagc
      961 ttctaaggaa aaacattttc ttgcagagtc tctcaataca gatggtagtc gtcagggact
     1021 tcaatctagt caagtcgggt taaattcacc tttctcagat gaagtacttg atgataaatt
     1081 tttgaaaatg gatggtagag caatctttga ttttgctatc aaagaagttt caaaaagtat
     1141 caatcatctt atagaaacat cccatttgga aaaagaagat attgattatc ttttcttaca
     1201 ccaagcgaat cgaaggattt tagataagat gtctcgaaaa attgatatag cacgtgataa
     1261 atttccagag aatatgatgg attatggtaa tactagtgca gctagtatcc ctattttact
     1321 ttcagaatct tatgagaacg gtttgttgaa actagatggt aaccaaacaa tcctcttatc
     1381 gggtttcggt ggaggtttga catggggaag tctaattgtt aaaatttagt tatatatagt
     1441 gcatagcaca tttataaaag attatttaaa atttaaggag ctaaaaatgg cagtatttga
     1501 aaaagtacaa gaaattattg ttgaagaact tggtaaagat gcagaagaag taacgttgaa
     1561 cactactttt gacgatcttg atgctgattc gcttgatgtt ttccaagtga tttcagaaat
     1621 tgaagatgca tttgatatcc aaattgagac agaagaagga ctaaatactg ttggtgattt
     1681 agttgcttat gttgaagaaa aagtaaaata attaagtcgt aagaaaaaag aagcaagtga
     1741 taataaaaga tattcttacc aaatcactta ttagagtgag tattcaatca atcgttgatc
     1801 ggtactcact cttcttctta tcaatatagt ttgattatca aattattaat tttaggaaag
     1861 aaaaatgaaa acacgtataa cagaattact aaatataaaa tatccaattt ttcaaggggg
     1921 tatggcgtgg gtcgctgatg gagaccttgc aggagcagtt tctaaggcag gtggtctagg
     1981 tattattggt ggaggaaatg cccctaaaga ggttgtcaaa gcaaatattg ataaaataaa
     2041 atctatgact gataaaccct ttggtgtgaa tattatgctt ttatcacctt ttgtagatga
     2101 tattgttgat ttggttattg aagaaggtgt aaaagttgta acgacgggtg caggtaatcc
     2161 tggtaagtac atggagcgct ttcatgaagc aggtataaca gtaatccctg ttgttcctag
     2221 tgttgcatta gcaaaacgta tggagaaatt aggggctgat gctatcatta cagaaggaat
     2281 ggaagcggga ggtcacatcg ggaaactgac cacgatgaca ttagtgcggc aggttgttga
     2341 tgctgttaca attccagtca ttgccgcagg tggtatagca gatggacgtg gggctgctgc
     2401 gggtttcatg ttaggagctg atgctgttca gataggaact cgttttgttg ttgctaaaga
     2461 atctaatgct caccctaatt acaaagcaaa aatcttaaaa gcaaaagata tcgacactgc
     2521 tgtttctgct caggtagtag gtcacccagt tcgtgcctta aaaaataaac tagtaacaac
     2581 ctatagccaa gcagaaaaag attatttagc tggccgtatt tcaatcaatg agattgaaga
     2641 attaggagca ggagctcttc gcaatgcagt tgttgatggg gatgttatta atggttcagt
     2701 tatggcagga caaattgccg gattaattaa atcagaagag acttgtcaag agattcttga
     2761 agatatttat tcaggagcgc gtcaagttat tctttctgag gcaaaccgct ggtcagattt
     2821 ataaatacaa ataaggagac actatgaata aagtcgcatt tctatttgct ggtcaaggtg
     2881 ctcaaaagtt gggaatggct agagatttat atgaaacttt cccgattgtt aaagaaactt
     2941 ttgataaggc aagtcatgtt ttaggatacg atttgagaga attgattgat aaagacttag
     3001 acaaattaaa tcagacgaaa tatactcaac cagctatttt aacgacttcg actgctattt
     3061 atcgtttaat tcttaaagaa attggactta gaccagatat ggttgcagga ctttctttgg
     3121 gagaatacag tgctttagtt gcttcaggtg ctataagatt tgaagatgct gtagtacttg
     3181 ttgctagacg aggtcaacta atggaagcag cagcacctgc tggtagtggc aaaatggtag
     3241 cggttttaaa tgctgatcgt caaataattg aagatgcttg taagaaggct tctcagtttg
     3301 gtattgttag tcctgcaaat tataatactc caaagcaaat tgttataggg ggagagtcaa
     3361 tcgctgtaaa tgcagctgta gaagaactaa aacaacaagg agtaaaacgt ttaattcctc
     3421 tgaatgtttc aggtcctttt cacactgctt tattgaaacc tgcaagtcag aaattgagcg
     3481 atgttttaga taaagttcat ttttcagttt ctgaaatccc tgtaattgga aatacagagg
     3541 cgcagataat gaaaaaagac gatatcaaat ctttattggc acgccaagtt atggaacctg
     3601 tacgctttga tgagtctatt gaaaccatga aaaaaatggg aatgacacag gttgttgaaa
     3661 ttggtccagg gaaggtactc tcaggtttct tgaaaaaaat agatagctca ttatcagtat
     3721 attctgttga agataaaatt ggatttaata atcttaagga attaaactag gggaaaatat
     3781 gcaattaaaa gataagaaca tttttattac gggttcaagt agaggaatcg gccttgcaat
     3841 tgcccatcaa tttgcacaat taggtgctaa tattgtttta aatggacgtt ctgaaatatc
     3901 agaagactta atagctgaat ttgctgatta tggtgtaaaa gttattgcta tttcaggaga
     3961 tgtttcttct tttgaagatg ctaatcgcat gattaaagaa gctattgcaa gtctaggtag
     4021 cgtggatgtg ctcgttaaca acgctggtat tactaatgat aaattgatgc ttaaaatgac
     4081 tgttgaagat tttgagagcg ttcttaaaat taatttaaca ggtgcattta atatgacaca
     4141 atcagtcttg aaaccgatga caaaagcgcg tcaaggtgct attattaata tctctagcgt
     4201 cgttggatta actggaaatg tagggcaagc taattacgca gcctctaaag caggtttaat
     4261 tggctttaca aaatcagttg ccagagaagt agcagcacga ggcattcgtg tcaatgccat
     4321 tgctccaggc tttatcgaat ctgatatgac agatgttatc ccagaaaaaa tgcaagaagc
     4381 tattttagct caaattccaa tgaaacgtat tggaaaaggt aaagaagtag cgcaggtagc
     4441 gtctttctta gcagaacaag aataccttac aggacaagtt attgccattg atggcggtat
     4501 gacgatgcaa taataaagag gaaataatat gacattacag cgagtagtag taacaggtta
     4561 cggtgtaacc tcacctattg gaaacacacc agaagaattt tggaatagcc ttaaagaagg
     4621 aaatgttggt attggaccta tcactaaatt tgattcaagt gatttcatgg tcaaaaatgc
     4681 tgctgaaatt catgattttc catttgataa atattttgtc aaaaaagatc tgaacagatt
     4741 tgatatgtat tccctttatg ccttatatgc ttcaagtgaa gctattcaac atgctaattt
     4801 aaatttagat gaaattgatg cagatcgatt tggtgtcatc gttgcatcag gtattggagg
     4861 gattcaagag attgaagaac aagtgattcg tcttcatgaa aaagggccaa aacgcgtaaa
     4921 accaatgacg cttccaaaag ctctaccaaa tatggcagct ggaaatgttg cgatgaggtt
     4981 aggcgctcat ggtgtatgta aatcaattaa tacagcttgt gcttcttcta atgatgctat
     5041 tggagatgct ttccgtaata taaaatttgg tattcaagat attatggtag tcggtggtgc
     5101 agaagctgct attacaaaat ttgccattgc tggattccaa agtcttacag cattatcaac
     5161 aactgaagat ccatcaagag catctattcc atttgataaa gatcgcaatg gatttatcat
     5221 gggagaaggt tctggaatgt tggtcttaga aagtttagga cacgctgaaa aacgaggagc
     5281 tactattctt gcagaagttg ttggttatgg taacacttgt gacgcatatc atatgacttc
     5341 accacatcca gaaggtttag gagccactaa agctattcaa cttgcactgg ctgaagctaa
     5401 tattaaacct gaagaagtta actatgtaaa tgctcatgga acttcaacac ctgctaacga
     5461 aaaaggagag agtcaagctg ttgttgctgc acttgggaca gacgttccag tttcttcgac
     5521 taaatctttt actggtcatc ttttgggtgc cgcaggagct gttgaggcaa ttgcaactat
     5581 tgaagcgata cgtcatagtt atgttccaat gactgctgga acaacagaat tatcagaaga
     5641 tattacagcg aatgttattt ttggtcaagg gcaagacgct gatattcgtt atgccatttc
     5701 aaatacattt ggttttggag gacacaatgc agttctggcc ttcaaacgct gggaggatta
     5761 aattggatat tcaagagatt aaggatttaa tgactcaatt tgatgagtca agtctgagag
     5821 aattttcatt caaaacaagt gacggtgagc tgtcttttag taaaaatgag ggaaaagctc
     5881 cacttgtccc aactatgtct catatgagtc atcaacctga agcaactcca actatagcaa
     5941 ctcctgtctc taatgaagca ggtgagcaaa cgaaacaagc tactgaagta gtttcagaaa
     6001 tcccagagtc gactgtgact gttgcagaag gtgacgttgt cgaaagtcct ttagttgggg
     6061 ttgcttattt agcttctgga ccagataaac ctaactttgt ttcagtaggt gatagcgtga
     6121 agaagggaca aactttgatg attattgagg cgatgaaggt gatgaatgaa gtgcctgctc
     6181 ctcatgatgg tgtagttact gaaattcttg tagccaatga agaagttatt gaatttggta
     6241 aaggattggt acgaatcaaa tgattgatat aaaagaaatt cgtgaggctt tacctcaccg
     6301 ctatccaatg ttattagtgg atcgtgtttt agaggtatca gaagatgaga tagtcgctat
     6361 caaaaatgtt agtatcaatg agcctttttt caatggtcat tttccagaat atcctgtaat
     6421 gccaggcgtt ttaattatgg aagctttggc tcaaactgca ggtgttttag aattatctaa
     6481 agaagaaaac aaaggaaagc ttgtttttta tgcaggtatg gataaagtga aatttaaaaa
     6541 gcaagttgtt ccaggagatc aattagttat gactgctaag tttgtcaaac gtagaggaac
     6601 tatagccgtt gttgaagcag tagcagaagt tgatgggaaa ttagctgcca gtggaacgtt
     6661 aacatttgca ataggcaatt gaagatagta ttgaatttag agggagagaa gcatcaaaaa
     6721 tgtttaagaa aatattgatt gctaatcgcg gagaaattgc agtacgtatc attcgtgcag
     6781 cccgtgaaat gggaatttcg acagttgcta tctattcgga ggctgacaag gaatcattgc
     6841 acacaatttt agcagatgaa gctatttgtg ttggaccagc caaatcagca gaatcttatt
     6901 taaacgttaa tgctatatta tctgctgcta ttgtgactgg agcggaagct gttcatccag
     6961 gttttgggtt tttaagtgaa aattctaaat ttgctactat gtgtgaagag atgaatttaa
     7021 aattcattgg tccatctgga gaagttatgg ataaaatggg tgataaaatc aatgctcgtg
     7081 cagagatgat aaaagctgat gttccggtta tcccaggatc agatggtcaa gttacaagtg
     7141 tagaagaagc tgtatcaatt gctgaggaaa tcggctatcc attgatgtta aaagcctcag
     7201 ctggtggcgg tggaaaagga attaggaagg tcaaatcagc agatgaattg aagcctgctt
     7261 ttgagtctgc ttcacaggag gctttggctg cgttcggtaa tggtgctatg tatattgaaa
     7321 aagttatata cccagctaga catattgaag tacaaatact tggagatagc tttggtaaga
     7381 tagttcacct aggagaacgt gattgttctc tgcagcgtaa caatcagaag gtattggaag
     7441 aaagtccgtc tgtggcaata ggtaacactc tgcgtcaaca aattggtgaa gcagctgttc
     7501 gtgcagctga ggctgtatcc tatgaaaatg caggaacaat tgagtttttg ttagatgaga
     7561 atagcggaca gttctatttc atggaaatga atactcgtgt tcaagtagaa caccctgtta
     7621 cagaatttgt aacgggtgtt gacatcgtta aagagcagat tcgtatcgct gctggaattc
     7681 ctttatcagt ttctcaaaat gatattaagt taactggtca tgcgattgaa tgtcgtatta
     7741 acgcagagaa tccacaattt aattttgcgc catgtccagg taccattaat gggttacatc
     7801 tacctgctgg cggtatgggc ttacgtgtcg attcagcggt ttacacaggc tacactattc
     7861 ctccttatta tgatagtatg attgcgaaag taattgttca cggagaaaat cgttttgatg
     7921 cacttatgaa aatgcaacgt gccttatatg agcttgaaat tgatggtatt gtaacaaata
     7981 cagaatttca aatggatctc atttctgata aaaaagtctt ggctggggat tatgatactt
     8041 cattcctgat ggaagacttt ttaccaaggt attatggtaa caatcaatag gagggaagat
     8101 ggctttattt agtaaaaagg ataaatatat ccgtattagt ccaaacaaag cattggggtc
     8161 ttctgataaa cgtagtcttc ctgaggttcc tgatgaactc tttgctaagt gtccttcttg
     8221 taaacatatg atttatcaga aagatttagg attagcaaag atttgtccag cttgttctta
     8281 caactttaga atttcagcgc aagaacgtct gttgctaaca gttgacgaag atagttttga
     8341 ggaattattt acagggattg aaactaagga tcctttgaat tttccaaact accgtgaaaa
     8401 actggctgct acacgtcaaa agactaactt agatgaagcg gtggtgacag gtttagctaa
     8461 aataaaagga caaactacgg ccttagctat tatggattct cattttataa tggcgtctat
     8521 gggaacggtc gtaggcgaaa agttgacaag attatttgag ctagcaactg aaaagaaatt
     8581 acctattgtt atttttacag cgtcaggagg cgccagaatg caagaaggta ttatgagttt
     8641 gatgcaaatg gcaaaagtat cggcagcagt taaacgccat tctaaccagg gattgtttta
     8701 tttaacaatt ttaacagacc caacaaccgg cggcgttact gcgagttttg ctatggaagg
     8761 tgatattatt ctggctgagc cgcaagcgtt ggttggtttt gctggtcgtc gtgttatcga
     8821 aacaacggtg agagaagatt tacctgaaga ctttcaaaag gcagaatttt tgttagaaca
     8881 tggttttgtc gatgctatta taaatcgaac agaattacgc gactgcattg ctcaactaat
     8941 tgctttccat ggaaatgagg ttggtcatga ataatgatgt gacaagaatt ttaaaagatg
     9001 cgcgtgatca agggaggcta actgctcttg actacgctga attgattttt gataatttta
     9061 tggaacttca tggtgatcgc cagtttgcag atgacaaatc aattatagga ggtttggggt
     9121 acttggcagg gcgtcctgtg acgattgtcg gtattcaaaa aggtaagaac cttcaagata
     9181 atttagaccg tcattttggt caaccacatc ctgaaggtta tcgtaaggct ttacgtttaa
     9241 tgaaacaagc tgagaagttt ggaagacctg ttataacctt catcaatact gctggagcat
     9301 atcctggggt aggtgctgag gagcgaggtc agggtgaagc aattgctaga aacctcttag
     9361 aaatgagtga tttaaaagtc cctattatag ctattattat tggtgaaggg ggttctggag
     9421 gagcattagc tttagctgtt gccgataagg tttggatgtt ggaacatact gtttattcta
     9481 ttttaagtcc tgagggcttt gcttctattt tatggaaaga tggcacaaga acaacagaag
     9541 cagcacaatt aatgaagatg actgctggag aactttacca tatggaagtt gtagacaaag
     9601 taatccctga acatggttat ttttcaagtg agattgtcga tatgattaag acaagtttaa
     9661 tttcagagtt agaaatgctc tcacaattat ctcttgagga tttattggaa caacgttacc
     9721 aacgttttag aaaatattaa tagaaaaagg ctgagttatt tactcagcct atatttagtt
     9781 tttcaaggta ttatcatagt tatagtttca tattttgcta taattgataa aaaggagata
     9841 tgtcaatgac actagcatta gccaattttt agattgtagt ctaatccaaa attttcagaa
     9901 agagtcattt cgaacacttt att
//
LOCUS       DATRTN010000066         9810 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.066, whole genome shotgun sequence.
ACCESSION   DATRTN010000066 DATRTN010000000
VERSION     DATRTN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.066"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            183..1007
                     /locus_tag="VBE22_001517"
     CDS             183..1007
                     /locus_tag="VBE22_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEO7202962.1"
                     /translation="MKEMTVSEIREVQLEMLAYIDKVARDNKIEYSLGGGSLLGAMRH
                     KGFIPWDDDIDLMLERSQYERLMKALADANNSDFKLLHHSVEKNLWPFAKLYHTKSMY
                     LSKTDRIHPWTGIFIDIFPLDRLPESAEERQRFFKKVHSAAANLMCTTYPNFASGSRK
                     LYANARLILGLPRFIAYHGQAKKRAEIVDQVMETYNNQEVPYMGYTDSRYRLKEYFPR
                     EIFSEYEDVMFENIKTRKIKNEHAYLNQLYGGSYMELPPESKRESHSYYTWYWKEG"
     gene            1007..1729
                     /locus_tag="VBE22_001518"
     CDS             1007..1729
                     /locus_tag="VBE22_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEO7202963.1"
                     /translation="MMNIGVIFAGGVGRRMNTKGKPKQFLEVHGKPIIVHTIDIFQNT
                     EAIDAVVVVCVSDWLDYMNNLVERFNLTKVKAVVAGGETGQMSIFKGLEAAEQLATDD
                     AVVLIHDGVRPLINEEVINANIQSVKETGSAVTSVRAKETVVLVNDSSKISEVVDRTR
                     SFIAKAPQSFYLSDILSVERDAISKGITDAIDSSTLMGMYNRELTIVEGPYENIKITT
                     PDDFYMFKALYDARENEQIYGM"
     gene            1729..2787
                     /locus_tag="VBE22_001519"
     CDS             1729..2787
                     /locus_tag="VBE22_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO7202964.1"
                     /translation="MMIKFSNKIVQEDLALISNQNYINWERIANKGVLISGSNSMLAS
                     YMVFLLAYLNETRNYQTQIIATARNIEKARDKFSDLVGKDYFTLIPYDVEERLEYDGK
                     VDYIIHAASNASPTAILSNPVSIIKANTIGTLNLLDFAKEKTIENFLFLSTREVYGTS
                     IKEVIDEEAYGGFDILATRACYPESKRMAETLLQSYYDQYKVPFTIARIAHSFGPGME
                     LGNDGRIMNDLLSNVIDGKDIVLKSSGTAERAFCYLADAVSGLFTILLNGEVGQAYNV
                     ANEDQPIMIKDLAQKLVDLFSDKNISVVFDIPKTMSAGYSKMGRTRLTMAKLEALGWK
                     REVSLESGILKTVQAFEE"
     gene            2789..3724
                     /locus_tag="VBE22_001520"
     CDS             2789..3724
                     /locus_tag="VBE22_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7202965.1"
                     /translation="MVKTAVLMATYNGEKFISEQLDSIRQQTLKPDYVLLRDDCSTDE
                     TVNVVNNYIAKHELEGWKIVKNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDIWYL
                     DKNERQFAIMSDKPQIEVLSADVDIKTMSTEASVPHFLTFSSSDRISQYPKVYDYQTF
                     RPGWTIAMKRDFAQAIADNWTFGANISHDNLIETTASIIGNGYNLNESVGVHIRYGGN
                     ASGNSKFVLNWKSPKTQHITGLKTFVDFYETMQKVVNKYQNDVALNQRLAFEKERLEA
                     SKLSLIHNSAFVIKNWRLYNSNSGRMRDLLFAFKK"
     gene            3733..4680
                     /locus_tag="VBE22_001521"
     CDS             3733..4680
                     /locus_tag="VBE22_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7202966.1"
                     /translation="MVKTAVLMATYNGQGFIHDQLDSIRNQTLRPDYVLMRDDGSTDD
                     TVKVVEDYIKEHRLDGWSITSNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDTWYH
                     HKNKMQVDIMEERQDINLLSADIDIKKLSEEATIPNNFKFNGEETISKYPLDFSYHNY
                     RQGWTFCLRKSFVDQVMTFYSDNLILSHDNLFAGVSGVIGSGYNLNRSVGEHKRHAEN
                     ASGNILNMSSSYERHLQELYFVLSYYIILVPFLEKSSSSQLREAKAYLDFNQQRYKNA
                     QNKDFVAILKQMMTKKRFYDSFSNRIRDLVFLLKDIKRS"
     gene            4682..6046
                     /locus_tag="VBE22_001522"
     CDS             4682..6046
                     /locus_tag="VBE22_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEO7202967.1"
                     /translation="MNLKKVRLFEKFFLDKNLHKTYFILGLTITIVLSLLMPFFNEPD
                     GQYHLAVSGRISNNIIDTSYYGEYNIDSGMRRQSTSYRDGTRFEKYYLNKANFVDGNH
                     LPREINYSKHNFVFWGHLVPAVGLFLGKFVYPSMGVMITVARLFSSFVSITLVALIIR
                     YVKKGKLIYFAIFLSPVVLNSFASLSYDSTGYIVFGAFLSLLINLLVDKSLTKNRSIA
                     LIGLILLVIVACKQNYWLFLLLVIPVLLQVNNPKLMGYNQKLSHLFQGLKSKKLFWLC
                     LTFLSLAIVYVLTARHGGVLAVLRRYVMTFGYNYAGINILSNDITSWLASPYPSQNFI
                     PTWVSSVWYLMVFLVWFSEEKFVKSKNMGFLFLASFFVGILGVFFIMLEYRGAPTSYI
                     EGVQGRYFTVTLLLVQVFASSIPARLNEAGRKVVPYALTGLVIVSNALLLFDTVISLI
                     MRRN"
     gene            6046..7470
                     /locus_tag="VBE22_001523"
     CDS             6046..7470
                     /locus_tag="VBE22_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEO7202968.1"
                     /translation="MKLLKNMFYNTSYQLLTLLLPLVTVPYVSRVLSPQGIGINAYTS
                     SIVMYFTLFGALGISLYGNREIAFVQSNKYKRSKIFWELVVLKLASVSIATLLFFGFV
                     LLTNEWQLFYLIQGINLLATATDISWYFIGVEDFKIIVIRNTIVKLITVVLTFLVVKT
                     PDDLALYMFLIAFASLLGNLTVWHHLKHEIIKIPFSRLDILIHLRPTLMLFLPQITMQ
                     IYLSLNKSMLGAMDSVVSAGYFDQSDKIIRILFTIVSAIGGVFLPRLSSLFSSGKEKQ
                     AKALLLKLVDLSNAISMLMIAGVVGVSSTFAVFFFGKGYEAVGPLMAVESLMIICISY
                     GNALGTQYLLASRRTKAYTMSAVIGLVANVVLNILLIPILGAMGAIISTVITEFIVSL
                     YQAISLRDVFTFKELTRGMLRYLIAATLSGAVLYYINTQMSVSLVNYVIQSLVAVTIY
                     VGIVFITKAPVIQLFRDFRKEHRT"
     gene            7467..8315
                     /locus_tag="VBE22_001524"
     CDS             7467..8315
                     /locus_tag="VBE22_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7202969.1"
                     /translation="MKYLAGIVTFNPNIERLDQNIRAIYPQVSHIYIVDNGSKNKEEI
                     SQLVADYNEEGHLTVDYLTENKGIAYALNCIGQFAVAQEFDWFLTLDQDSVVLGDLID
                     NYENYLHLPKVGMLSCLYQDMNRENLVMQEFDYKEIEECITSAALMKTSVFEETSGFA
                     EEMFIDFVDSEMNYRLSEMGYKTYQVNFIGLLHEIGHSSRVKKFGHVFHVLNHSPFRK
                     YYMIRNAIYIIKKYGKKKRYKYLVFMRNEFVRVLVAEEQKSKKIVAMIKGLKDGLLMK
                     VDKYGK"
     gene            8305..9444
                     /locus_tag="VBE22_001525"
     CDS             8305..9444
                     /locus_tag="VBE22_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO7202970.1"
                     /translation="MENKVKTVAVFSGYYLPFLGGIERYTDKMTADLVKRGYRVVIVT
                     TNHGDLPIIDEDKGRKIYRLPTKNIVKQRYPIINKNREYNTLMKYVSDENIDFVICNT
                     RFQLTTLEGLSFAKKHHLPSIVLDHGSSHFSVNNRFLDFFGAIYEHLLTARVKHYRPD
                     FYAVSKRSVEWLKHFNIEAKGVIYNSVSESLGSDFADTAYLEKSADDIFITYAGRIIK
                     EKGIELLLEAFLMSQYSENVYLQIAGDGPELAHLKEKYQSKQINFLGKLNFEQTMSLM
                     AQTDIFVYPSMYPEGLPTSILEAGLLSSAIIATDRGGTVEVIDSPELGIIMEENTQSL
                     HESLDLLVKDKALREKLQQNIAKRIKEHFTWEKTVEKLDYIIQKN"
BASE COUNT         3192 a         1424 c         1882 g         3312 t
ORIGIN      
        1 tgcagtacaa caaacaagaa tacaatgcac ttgcaaacac tccaaaaggt caaaaaacaa
       61 agaaaacttt caaacccatg accctagtga ttctttttgc agtagcttta tttattttct
      121 atattacagc tataagattt gtatttaaaa gaatgtgagt taactgttag acagaggtaa
      181 ttatgaaaga aatgacggtc tcagaaataa gagaagttca acttgaaatg ttagcttata
      241 ttgataaagt tgcgcgtgat aataaaattg aatattcttt aggcggaggc agcctactgg
      301 gagcaatgag acataaagga ttcattccat gggatgacga tattgatttg atgctagaaa
      361 gaagccagta tgagcgtttg atgaaagcat tagctgatgc taataatagc gattttaaat
      421 tgctacatca ttctgttgaa aaaaatctat ggccatttgc caagctgtat cacacaaagt
      481 caatgtactt aagtaaaaca gatagaattc atccttggac agggattttt attgatatct
      541 tcccacttga ccgattacca gagagtgcag aagaacgtca aagattcttt aaaaaagttc
      601 actctgctgc tgcaaatttg atgtgtacga cttaccccaa ttttgctagc ggttcccgaa
      661 agctttatgc aaatgcaagg ctaattttgg ggctaccccg attcatcgct tatcacggcc
      721 aagctaaaaa acgtgctgaa attgttgatc aagtgatgga aacatataat aatcaagagg
      781 ttccttacat gggttatacc gattcaagat atcgcttgaa agaatatttt ccaagagaaa
      841 ttttttctga gtatgaagat gttatgtttg aaaacattaa aacccgaaaa attaaaaatg
      901 agcatgctta tctgaatcaa ttatatgggg gtagttatat ggaattacct ccagaaagta
      961 agagagaaag tcatagttat tacacttggt actggaaaga aggttaatga tgaatattgg
     1021 agttattttt gcaggtggtg ttggtcgtcg catgaatacc aaaggtaaac caaagcaatt
     1081 cctagaagtg catggtaaac ccattattgt acacacaatt gatattttcc aaaatacaga
     1141 agctattgat gctgtagtag ttgtctgtgt atcagactgg ctagattata tgaataatct
     1201 ggtagagcgt ttcaacctta cgaaagtaaa agcagttgtt gcaggtggag aaactggcca
     1261 aatgtcaatt tttaaggggc ttgaagccgc tgaacagctg gctacagatg atgccgttgt
     1321 tttaattcat gacggagtac gtccattgat taatgaagaa gttattaatg ctaatataca
     1381 gtcagtaaaa gaaacaggaa gtgcggtaac ttcagttcgt gctaaagaga cagttgttct
     1441 agtcaatgat tcttctaaaa tatctgaagt tgttgatcgt actcgttcct tcattgctaa
     1501 agcgccacaa agtttttatc tatcagatat cctttctgtt gaacgtgatg ccattagcaa
     1561 agggattact gatgcaattg attctagtac tttgatgggt atgtacaatc gtgaactcac
     1621 tattgttgag ggaccatatg aaaatatcaa gataacaaca ccagatgatt tttatatgtt
     1681 taaagctttg tatgatgcta gagaaaatga acaaatttat gggatgtaat gatgattaaa
     1741 tttagtaata aaattgtaca agaagattta gcacttatta gtaaccaaaa ttatattaat
     1801 tgggaaagaa ttgctaataa aggcgtttta ataagtggtt ctaatagtat gttagcgagt
     1861 tatatggtat ttttactagc ctatctcaat gagacaagaa attaccaaac gcaaattatt
     1921 gcgacagcta gaaacattga aaaagcccgt gataaatttt cagatttagt tgggaaagat
     1981 tatttcacct taattcctta tgatgtagaa gaacgcctag agtatgatgg aaaagtggat
     2041 tacattatcc atgcagcaag caatgctagt ccaactgcta ttttgagtaa tcctgttagc
     2101 atcataaaag ctaatacaat tggtacattg aatttattag actttgcaaa ggaaaaaaca
     2161 attgaaaact tcttgttctt atcaacgcgt gaagtatatg gaacatctat taaagaagtg
     2221 attgatgaag aagcttatgg tggatttgat attttagcta ctagagcttg ttacccagaa
     2281 agcaaacgta tggctgaaac tctactgcaa agttactatg atcaatataa ggtacccttt
     2341 actattgctc gtattgcaca ttcatttggt cctggtatgg agcttggtaa cgatggtcgt
     2401 attatgaatg atcttttatc gaatgtcatt gatggcaaag atatcgtttt aaaaagtagt
     2461 ggtacagcag agcgtgcttt ctgttattta gcagatgctg tatcagggtt atttactatt
     2521 ttattaaatg gtgaagttgg tcaagcttac aatgtagcaa atgaagatca acccattatg
     2581 attaaggatt tggcacaaaa gttagttgat ctcttttctg ataaaaatat ttcagtagta
     2641 ttcgatattc ctaaaactat gagtgcaggt tatagcaaaa tggggagaac acgtctcact
     2701 atggccaaat tagaagcgtt gggatggaag cgagaagttt ctcttgaatc tggaatatta
     2761 aaaacagtac aagcatttga ggagtaatat ggtaaagaca gcagttttaa tggcgacata
     2821 caatggcgaa aaatttatat ctgaacaact tgattcaatt cgccaacaga cattaaaacc
     2881 agattatgta ttattgaggg atgattgttc aacggatgaa acagtcaatg tcgtcaataa
     2941 ctatatcgca aaacatgagt tagaaggctg gaaaattgtt aaaaacgaca aaaacttagg
     3001 ctggcgttta aattttcgtc aattacttat tgatgtgtta gcctatgagg ttgactatgt
     3061 cttttttagt gatcaagatg atatttggta tcttgataaa aacgaacgac agtttgccat
     3121 tatgtcagat aaacctcaaa ttgaggtttt gagtgcagac gttgatatca aaacgatgtc
     3181 tacagaagcc agtgttccac attttctaac tttttcttct agtgatagaa tcagtcagta
     3241 tcctaaagta tatgattatc aaacattccg tcccggatgg accattgcta tgaagagaga
     3301 ttttgcgcaa gctatcgctg ataattggac ttttggagct aatatttcgc acgataatct
     3361 tatcgagaca actgccagta ttattggaaa tggttataac cttaacgaaa gcgtaggagt
     3421 tcatattcga tatggaggaa acgcaagtgg taatagcaaa tttgttttga attggaagtc
     3481 gcctaaaaca caacatatca caggattaaa aacatttgtt gatttttacg aaaccatgca
     3541 gaaggttgtc aacaaatatc aaaatgatgt cgctttaaat caacgcttag cctttgaaaa
     3601 agaaagattg gaagcttcta aattgtcatt aattcataat agtgcttttg tgattaaaaa
     3661 ttggcgactc tataactcta atagtggacg tatgagagat ttattatttg cgtttaaaaa
     3721 gtaaggaata atatggtaaa aacagcagtt ttgatggcaa catataatgg tcaaggtttc
     3781 atccacgatc agctagattc aattagaaat caaacgttga gaccagacta tgtcttaatg
     3841 agggacgatg gctctactga tgatactgta aaggtggttg aagactatat aaaagagcac
     3901 cgtttagatg gttggtcaat aactagtaat gataaaaatt taggttggcg tttaaatttt
     3961 cgtcaattac ttattgatgt gttagcctat gaggttgact atgtcttttt tagtgatcaa
     4021 gatgacacat ggtatcatca taaaaataag atgcaagttg atattatgga agaacgccaa
     4081 gatataaacc ttcttagcgc cgatattgat atcaaaaagt tatcagaaga ggctactatt
     4141 cccaataatt ttaagttcaa tggtgaggaa acaatttcta agtatccatt agacttttca
     4201 tatcataact atcgtcaagg ttggactttt tgtttacgca agtcgtttgt tgatcaagta
     4261 atgacttttt acagtgataa tttaattttg tcacatgata atctttttgc aggggtatct
     4321 ggtgtcattg gttcaggtta caatttaaac agaagtgttg gtgaacataa gcgacatgct
     4381 gagaatgcaa gtgggaatat cttgaatatg agctcaagtt acgaaaggca tttacaagaa
     4441 ctgtattttg ttttgagtta ctatatcatc cttgtaccat ttttagaaaa aagtagctct
     4501 agtcaattaa gggaagctaa agcttatctg gattttaatc agcaacgtta taaaaatgct
     4561 caaaataaag attttgtagc aatacttaaa caaatgatga ctaaaaaacg gttctatgat
     4621 agcttttcaa atcgcattcg tgacttggtg tttttattaa aagatataaa aaggagttaa
     4681 tatgaacctc aaaaaagttc gtctatttga aaagtttttt ctagacaaga acttgcataa
     4741 aacatatttt atattaggct taacgattac tattgtattg agcctattaa tgccattttt
     4801 caatgaacct gatggacaat accatttagc tgtgtctggt cgtatttcaa ataacattat
     4861 tgatacttct tactatggag aatataatat tgattcaggt atgagacgac aatcgacctc
     4921 ctatagagat ggtactagat ttgaaaaata ctatttaaat aaagccaatt ttgttgatgg
     4981 taatcatttg cctcgtgaga taaactattc taaacataac tttgtttttt ggggtcatct
     5041 tgtgccagca gttggcttat ttttaggcaa atttgtgtat ccttctatgg gggtaatgat
     5101 tacagtagca aggttattta gttcctttgt ctcaatcacc ttggttgccc tcattatccg
     5161 gtatgtgaaa aaaggaaagt taatctactt tgctattttc ctcagtcccg tagttctaaa
     5221 ttcatttgct agtctatctt atgactcgac tggatatatt gtatttggag catttttatc
     5281 gcttttgata aatcttttag ttgataaatc gttgactaaa aatcgtagta ttgctcttat
     5341 aggattgatt cttttagtca ttgttgcatg taagcagaat tactggctgt ttttattgct
     5401 agtaataccg gtattattgc aggttaacaa tcctaaatta atgggctata atcagaagct
     5461 atcacattta tttcaaggtc ttaaatctaa aaagctattt tggctatgtc taaccttttt
     5521 gtcacttgct atagtttatg ttctgaccgc aagacatggt ggtgtccttg ctgttttaag
     5581 acgttatgta atgacatttg gctataatta tgctggtatc aatattttaa gtaatgacat
     5641 tacttcctgg cttgcctctc catatccaag tcagaatttt attcccactt gggtatcatc
     5701 agtatggtat ctcatggtct ttctagtatg gttttctgaa gaaaaatttg ttaagtcaaa
     5761 aaatatggga tttcttttct tagctagttt ctttgtggga attcttggtg tcttttttat
     5821 catgttagaa tatcgtggag cgccaacaag ctacattgaa ggagttcaag gacgttactt
     5881 tactgttaca ttgcttttgg tacaagtttt tgcatcaagt attccagcgc gtctaaatga
     5941 ggcaggaagg aaagtggtgc cgtatgcgtt aactggcttg gtaattgtat caaatgcctt
     6001 attgttattt gatactgtta ttagtttaat tatgaggaga aactaatgaa acttttgaaa
     6061 aatatgtttt ataatacctc ataccagtta ttgactttgt tgttaccttt agtaacagtt
     6121 ccttatgttt cccgtgtact atctcctcaa ggcatcggta ttaatgccta tacgagctcc
     6181 attgtcatgt actttacttt atttggtgct ttagggatat ctttgtacgg taatcgtgag
     6241 atagcttttg tgcagtcaaa taagtataag agaagtaaga tattttggga attagttgta
     6301 ttaaaacttg cctctgtatc tatagcaacg ttattattct ttggatttgt cttacttaca
     6361 aacgaatggc aattattcta tttaattcaa ggtattaatt tattagcgac tgcaactgat
     6421 atttcgtggt attttattgg agttgaagac tttaagataa ttgtcattcg taataccatt
     6481 gttaagctta ttacagttgt tttgaccttc ttagttgtga agacgccaga tgacctagca
     6541 ctttatatgt ttttaattgc atttgcatca ttactcggaa atttaacagt ctggcatcat
     6601 ttaaagcatg aaatcatcaa gataccattt agtagattag atatcttaat acatctaaga
     6661 ccaactttaa tgttattttt accccaaatc accatgcaga tttatctctc tcttaacaag
     6721 agtatgctgg gagcaatgga tagtgtggtc agtgctggat attttgatca atctgataaa
     6781 attattcgta ttttatttac cattgtttca gcaatcggag gagttttctt accaaggctc
     6841 tccagtctat tttcttcagg aaaagaaaag caagctaagg ctttactttt gaaacttgtt
     6901 gatttaagca atgctatctc aatgttaatg attgcgggag tggtaggagt ttcgtcgaca
     6961 tttgctgtct tcttttttgg gaaagggtat gaagctgtcg gtccgctaat ggctgttgag
     7021 tctttgatga tcatttgcat ttcttatggt aacgctttag ggacacagta tctactggct
     7081 tcaagacgta caaaagctta cacaatgtct gcagttattg gacttgtggc aaatgttgtg
     7141 cttaatatac ttttaatacc aattttaggt gctatgggag ctattatctc tacagttatt
     7201 actgaattta ttgtctccct atatcaagct atctctttaa gagatgtatt tacttttaaa
     7261 gaattaacaa gaggtatgct gcgctattta attgctgcta ctcttagtgg agctgtactc
     7321 tattatatca atactcaaat gtcagtatca ttggtaaatt atgtcattca aagtttagta
     7381 gcagtaacta tctatgtcgg tatcgttttt ataaccaaag caccagtcat tcagttattt
     7441 agagatttta gaaaagagca tagaacatga aatatttagc gggtattgtc acctttaacc
     7501 ccaatattga acgcttagat cagaacattc gagcaattta tcctcaagta agccacatct
     7561 atatcgtaga taatggctcg aaaaataagg aagaaatttc tcaattagta gccgattata
     7621 atgaagaggg gcacctaact gttgattatt taacagaaaa taaagggatt gcatatgctt
     7681 tgaactgtat cggtcaattt gcagtagcac aagaatttga ttggttcttg actttagatc
     7741 aagattcggt tgtcctaggt gatttgatag ataattatga aaattacctt catttaccta
     7801 aggtcggtat gttatcttgt ttgtatcaag atatgaaccg tgaaaattta gtgatgcaag
     7861 aatttgacta taaagaaatt gaagagtgta ttacttcggc tgctttaatg aaaacgagcg
     7921 ttttcgaaga aacatctggc tttgcagaag agatgtttat tgattttgtt gactctgaaa
     7981 tgaattatcg tctgtcagag atgggatata agacttatca ggttaatttt attggtctgt
     8041 tacacgaaat tggacactct tcacgagtta aaaaatttgg ccatgttttt catgtattga
     8101 atcattcacc atttcgaaaa tattacatga tacgcaatgc gatctatatt ataaaaaaat
     8161 atggtaagaa gaagcgatac aaatatttag tatttatgcg aaatgaattt gttcgagtgt
     8221 tggtagcaga agagcaaaag agcaaaaaaa tagttgctat gattaaaggc ttaaaagatg
     8281 gcttattgat gaaagtggat aaatatggaa aataaggtaa aaacagttgc tgttttctca
     8341 ggatattatc tacccttcct cggaggtatt gaacgttata cagataaaat gacagcagat
     8401 ttggttaagc gtggctaccg tgttgttatt gtaacaacta atcatggtga tttaccgatt
     8461 atagatgaag acaagggtag aaaaatttac cgtttaccga ccaaaaatat tgtcaagcag
     8521 cgctatccta ttattaacaa aaatcgagaa tacaacacgc tgatgaaata tgtttcagat
     8581 gaaaatattg attttgtgat ttgtaataca cgttttcagt taacaacgtt agaagggcta
     8641 tcttttgcaa agaagcacca tttacctagt attgttttag accatgggtc aagtcatttt
     8701 tctgtcaata atcgtttttt agatttcttt ggagctatct atgaacatct tctcacagcg
     8761 cgtgttaagc attatcgtcc agatttttat gcggtatcca agagaagcgt tgaatggtta
     8821 aaacatttta atatagaagc taaaggagtt atttataatt cggtatctga aagtctaggc
     8881 tctgattttg ctgacacggc ttaccttgaa aaatctgctg atgatatttt catcacttat
     8941 gctggtagaa ttattaaaga aaaaggcatt gaattgctct tagaagcatt tttgatgtca
     9001 cagtactctg aaaatgttta tttacagatt gcaggagacg gacctgaatt agcacatttg
     9061 aaagagaaat atcagtccaa acagattaac tttttgggga aattaaactt tgaacagact
     9121 atgtctttaa tggcacagac tgatatcttt gtttacccat caatgtatcc agagggatta
     9181 ccaacctcta ttttagaagc tggtctactt tcaagtgcta ttattgcaac agatcgcggt
     9241 ggaacagttg aagttatcga tagccctgag ttaggaatca tcatggaaga aaatactcag
     9301 tcactccatg aatctcttga tttattagta aaagataagg cattaagaga gaaactacaa
     9361 cagaatattg ctaaaagaat taaagaacat ttcacctggg aaaaaacagt tgaaaagttg
     9421 gattatatta ttcaaaaaaa ttaacattaa aaagctagag cattgtgtaa tgctctagct
     9481 ttttaatgtt aatttttaag agttatagga gctacgggta gttatttcat tgttctttgc
     9541 aaaaagttta caatattttt cttcttgtat tgatgaaata atttcttgga tttgattgat
     9601 ctggtctgga gtatgaatca aatcaatata tgtaattaaa ctctctgaag aaatctcatg
     9661 ttttataaag gaagaaagct ttgggttggt aataataata tctggattaa gattagtaat
     9721 attggaaaga tcatcagtta ataaatagta agggtggaaa tgaatctgtt tagaagaaaa
     9781 cctctgtgat aaacattctg ttaaaatttt
//
LOCUS       DATRTN010000067         9392 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.067, whole genome shotgun sequence.
ACCESSION   DATRTN010000067 DATRTN010000000
VERSION     DATRTN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.067"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            15..662
                     /locus_tag="VBE22_001526"
     CDS             15..662
                     /locus_tag="VBE22_001526"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEO7202971.1"
                     /translation="MVFANQELLDTAFTHTSYANEHRLLNISHNERLEFLGDAVLQLL
                     ISQYLFTKYPQKAEGDLSKLRSMIVREESLAGFSRLCGFDHYIKLGKGEEKSGGRNRD
                     TILGDLFEAFLGALLLDKGVEVVHAFVNKVMIPHVEKGTYERVKDYKTSLQELLQSHG
                     DVKIDYQVTNESGPAHAKEFEVTVSVNQENLSQGIGRSKKAAEQDAAKNALATLQ"
     gene            670..4209
                     /gene="smc"
                     /locus_tag="VBE22_001527"
     CDS             670..4209
                     /gene="smc"
                     /locus_tag="VBE22_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEO7202972.1"
                     /translation="MFLKEIEMQGFKSFADKTKVEFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFAGTENRKPLNYAQVSVTLDNSDHFIENIADEVRVER
                     RIFRNGDSEYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQSKLEQTQGNLDRLEDIIYELDMQVQPLEKQASIAKRFLVL
                     DEERQGLHLSILIEDILQHQSDLTTVEEKLLTVREELATYYQQRQSLEDENQSLKQKR
                     HHLSEEIEAKQLALLDVTKLKSDLERQIDLIRLESNQKAEKKEEAGQRLAELEIKAKD
                     CSDQITQKNIELTTLSEKIAQIRSEIVSTESSLERFSTNPDQIIEKLREDFVTLMQEE
                     ADTSNALTALLADIENQKQASQAKSQEIQEVSKNLEVLKSNAKVALERFEAAKKNVRR
                     LLSHYQDLGQTLQNLEGEYKNQQSILFDHLDEIKSKQARISSLESILKNHSNFYAGVK
                     SVLQAKDQLGGIIGAVSEHLSFDKHYQIALEIALGGSSQHIIVEDESAAKRSIAFLKK
                     NRQGRATFLPLTTIKPRELAQHYLSKLQSSQGFLGIASELVTYDQRLSNIFKNNLGLT
                     AIFDTVDNANVAARQLNYQVRLVTLDGTELRPGGSYSGGANRQNNTVFIKPELDNLKK
                     ELKQAQSKQLIQEKEVAALLEQLKEKQETLAQLKNDGEQARLEEQRADIEYQQLSEKL
                     ADLNKLYNGLQLSNGVSEQTTSQNEKNRLEKELEQFAIKKEELTTSIAQIKEDKDSIQ
                     EKVNNLTTLLSEAQLEERDLLNEQKFERANCTRLEITLSEIKRDISNLQTLLSHQDSQ
                     LDKEELPRIEKQLLQANKRRENDEEKLVSLRFELEDCEAALDDLAASLAKEGQKNESL
                     IRQQAQLESQCEQLSQQLMVFSRQLSEDYQMTLDEAKVKANVLEDILMAREQLKSLQA
                     KIKALGPVNIDAIAQFEEVHERLTFLNTQRDDLVHAKNLLLETITDMDDEVKTRFKST
                     FEAIRHSFKETFVQMFGGGSADLILTEGDLLSAGVDISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTISFVILDEVEAALDEANVKRFGDYLNRFDKSSQFIVVTHRKGT
                     MSAADSIYGVTMQESGVSKIVSVKLKEAQEMTN"
     gene            4300..5097
                     /locus_tag="VBE22_001528"
     CDS             4300..5097
                     /locus_tag="VBE22_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7202973.1"
                     /translation="MIKLVATDMDGTFLDENGTYDKKRLANVLKKFKEQGIVFTAASG
                     RSLLSLEQLFADFRDQMAFIAENGSAAVLFDRLAYEQHLSREQYLDIIDHLSKSPYME
                     NNEYVLSGKDGAYILSDANPGYIEFITHYYDNLQKVSHFEDVDDIIFKVTANFTEETV
                     RQAEEWVNQVIPYATAVTTGFKSIDIILSSVNKRNGLEHLCEQYGIRAEEVLSFGDNI
                     NDLEMLEWSGKAIATENARPEVKEIADCIIGHHNDQAVMAYLESMVD"
     gene            5097..5921
                     /locus_tag="VBE22_001529"
     CDS             5097..5921
                     /locus_tag="VBE22_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO7202974.1"
                     /translation="MSDIKILALDLDGTLFTTDKKVSEENKVALKAAREKGIKVVITT
                     GRPLKAIGNLLEDLELVSDEDYSITFNGGLVQQNTGKILAKTAMTRQEVEDIHEELYQ
                     VGLPTDILSEGMVYSIANKGHHSQYHLANPLLEFIEVDDLEQVPKDVVYNKIVSVIDA
                     TYLDQQIAKLPDRLKVDYEMFKSRDIILELMPKGVHKAVGLELLTKHLGLDSSQVMAM
                     GDEANDLSMLEWAGLGVAMANGIPEAKAIAKATTICNNDESGVAEAVGKYILSEEN"
     gene            5921..7531
                     /gene="ftsY"
                     /locus_tag="VBE22_001530"
     CDS             5921..7531
                     /gene="ftsY"
                     /locus_tag="VBE22_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEO7202975.1"
                     /translation="MGLFDRLFGHKKKDKEPEIEASESVVLEDEDSVIDKEEGSNFSK
                     ESTLNRTSEVPVAEDDSFLELERDTALSESHQPVTSEIHPLESEDTDEIPVKEDDSFL
                     ELEDRAKTKVADTSEVGNVVPDSTTLSDNVSAKSEASFSDKEQSSNSQASEQFSETPF
                     QEEMSSGKTEVQTESEDTSAADAFLADYYAKRKAIEKEISSNSLSTDESKLSEAQEVL
                     SQSQADTIKAESQEEKYNRSLKKTRTGFSARLNAFLSNFRRVDEEFFEELEEMLILSD
                     VGVNVATQLTEDLRYEAKLENAKKSEDLKRVIVEKLVEIYEKDGIYNEAINFQEGLTV
                     MLFVGVNGVGKTTSIGKLAHQYKSQGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVVT
                     GEEKADPASVVFDGMEKAIAQGVDVLLIDTAGRLQNKENLMAELEKIGRIIKRVVPDA
                     PHETLLALDASTGQNALSQAKEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVK
                     FIGFGEKIDDIGEFNSEDFMRGLLEGIL"
     gene            complement(7568..8380)
                     /locus_tag="VBE22_001531"
     CDS             complement(7568..8380)
                     /locus_tag="VBE22_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEO7202976.1"
                     /translation="MIRFLILAGYFELSMYLKLSGKLNQYINTHYTYLAYISMVLSFI
                     LAIVQLIIWVKNMKMHSHLHGKIAKSTSPMILVFPVLVGLLVPTVSLDSTTVSAKGYN
                     FPLAAGSTGTVSQDGTRVQYLKPDTSTYFTSSAYEKEMQKELKKYKGSGTLTITTENY
                     MEVMELIYLYPEQFMDRQIQYTGFVYNEPKHEGYQFIFRFGIIHCIADSGVYGLLTTG
                     NQKSYPDNTWVTVRGTIKSEYNQLLQQNLPVLHIEESRQVSKPNNPYVYRVF"
     gene            complement(8380..9282)
                     /locus_tag="VBE22_001532"
     CDS             complement(8380..9282)
                     /locus_tag="VBE22_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO7202977.1"
                     /translation="MDIFNQLPDSVLQWFAIFISIIIEALPFVLLGTILSGIIEVFIT
                     PDIVNKFLPKNKFLRVLFGTFVGFVFPSCECGIIPIINRFLEKQVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSIRFLILRFVGATIVAIALGVMLAFLVDDNILKEDAKPTHFH
                     DYSDKKWYQKIFLALAHAIDEFFDTGRYLVFGTLIASAMQIYLPTRVLTTIGHSPITA
                     ILVMMLLAFILSLCSEADAFIGASLLSTFGIAPVMAFLLIGPMIDIKNLMMMVNSFKT
                     RFIVQFISVSSIIIIIYCLFVGVI"
     gene            complement(9288..>9392)
                     /locus_tag="VBE22_001533"
     CDS             complement(9288..>9392)
                     /locus_tag="VBE22_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202978.1"
                     /translation="DDLESMFASFAKVPKPKKVDSDSKPEQKDDSQKD"
BASE COUNT         3332 a         1437 c         1897 g         2726 t
ORIGIN      
        1 aaaaagacta tggtattgtt tttgctaatc aagaattatt agatacagct tttacccata
       61 catcatacgc aaatgagcat cgcctcctaa acatttcaca taatgaacgt ttggaatttt
      121 taggagacgc tgttctacaa ttacttattt cacagtatct ttttacaaag taccctcaaa
      181 aggcagaagg tgatttatca aaattacgtt caatgattgt tcgtgaggaa agtcttgcag
      241 gtttttcacg tctatgtggc tttgatcact atattaaact tggaaaagga gaagaaaaat
      301 ctggtggacg taatcgtgac actattttag gagatttatt tgaggctttt ttaggagctt
      361 tattattgga taaaggtgtt gaagtggttc atgcctttgt taacaaggta atgatccctc
      421 acgttgaaaa agggacctat gagcgtgtta aagattacaa gacaagcttg caagaattac
      481 ttcaatctca tggggatgta aaaatagatt atcaagttac taatgaaagt ggtccagcac
      541 atgcaaagga atttgaggtg actgtatctg tcaaccaaga aaatttgagc caaggaattg
      601 gacgatcaaa gaaagctgct gaacaagacg ctgccaaaaa tgctttagca acgttacaat
      661 gaggaaatca tgtttttaaa agaaattgaa atgcaaggct ttaagtcctt tgcggataaa
      721 actaaggtag aatttgatca aggagttact gctgttgtag gtccaaatgg atcaggtaaa
      781 agtaatatca cagagagttt acgttgggca ttgggagaat caagcgcaaa aagtttacga
      841 ggaggtaaga tgccagacgt catatttgct ggtacagaaa atcgtaagcc ccttaattat
      901 gcgcaagtga gtgttacttt agataatagc gaccacttta tcgaaaatat agctgacgaa
      961 gttcgagttg aacgtcgtat ttttcgaaac ggagatagtg aatatttaat agatggacgc
     1021 aaagttcgct tacgtgatat tcatgattta tttatggata ctggattagg aagagattct
     1081 ttttctatta tttcccaagg acgcgttgaa gctattttta atagtaaacc agaagagcgc
     1141 cgtgccatat ttgaagaagc cgctggtgtt ctgaagtaca aaacacgtaa gaaggaaaca
     1201 caatccaaat tagaacaaac tcaagggaac ttagatcgct tagaggatat tatttatgaa
     1261 ttggatatgc aagtccaacc attagaaaaa caagcaagta ttgccaagcg ttttttagta
     1321 ttagatgagg aacgacaagg actccactta tctatcttga ttgaagatat tttacaacat
     1381 caatcagatt taactactgt tgaggaaaag ctattgacag taagagaaga gttagcaact
     1441 tattaccaac aacgtcagtc ccttgaagat gaaaatcaat cgttaaaaca gaaacgtcac
     1501 catttatctg aagaaattga ggctaaacag ttagctcttt tagacgttac aaagttaaag
     1561 tcagatttgg aacgccaaat tgatctgatt cgtttagagt caaaccaaaa ggctgaaaaa
     1621 aaagaagagg caggtcaacg tttagcagag ttagaaatta aagctaaaga ttgtagtgac
     1681 cagattaccc aaaaaaatat agagttaact actctgtcgg aaaaaatagc ccaaataaga
     1741 tcagaaattg ttagtacaga aagttccttg gagcgttttt caactaatcc ggatcagatt
     1801 attgaaaaac ttcgagaaga ctttgttacc ttgatgcaag aagaagccga cacttcaaac
     1861 gctttaacag ctttactagc tgatatagag aatcaaaaac aagcttctca agctaaatct
     1921 caagaaatac aagaagttag caaaaattta gaagtattaa aaagtaacgc aaaggtagcg
     1981 ttggaacgat ttgaggctgc aaagaagaat gttcgtcggt tattatctca ctaccaagat
     2041 ttaggtcaaa ccttgcaaaa tctagaaggt gagtataaga atcagcagtc aattttattt
     2101 gatcatttag atgaaatcaa atcaaagcaa gcacgtattt cgagtttaga atctattttg
     2161 aaaaatcata gtaatttcta tgctggagtg aagtcagttc tacaggctaa ggaccagctt
     2221 ggaggtatca taggtgctgt tagtgagcat cttagctttg acaaacatta ccaaatagca
     2281 ttggagatag ctttgggagg tagcagccaa cacatcattg ttgaagacga gagtgctgcc
     2341 aaacgttcaa ttgctttttt aaagaaaaac cgccaaggta gagcgacatt tctgccttta
     2401 actacaataa aaccaagaga attagcacaa cattatctct caaaattaca aagttctcaa
     2461 gggtttttag gaatagctag tgaattggta acctatgatc aacgcttgtc aaacattttt
     2521 aaaaataact tagggttaac tgctattttt gatacggttg ataatgctaa tgtagctgca
     2581 agacaattga attatcaagt acgtttagta accttagatg gaacagaatt acgccctgga
     2641 gggtcgtact cagggggagc aaatcgtcaa aataatactg tttttatcaa gcctgaattg
     2701 gataatctaa aaaaagaatt aaaacaggca caaagtaaac aattaattca agaaaaagaa
     2761 gttgccgcat tgttagagca actcaaagag aaacaagaaa ccttagcaca acttaagaat
     2821 gatggagaac aagctcgtct tgaggaacaa agagctgata tagagtatca acaactatca
     2881 gaaaagcttg ctgatttaaa taaactatat aatggcttac aattaagcaa cggggtgtcg
     2941 gaacaaacta cttctcaaaa tgaaaaaaat cgtttagaaa aagaacttga acaatttgct
     3001 attaaaaaag aggagttgac aacttcaatt gctcaaatta aggaagataa agattcaatt
     3061 caagaaaaag tcaataatct aacaacatta ttgtcagagg cacagctaga agaacgtgat
     3121 ttgctaaacg agcagaaatt tgaacgtgcc aactgtacac gtctagagat taccttgtct
     3181 gaaataaaac gtgacattag taatttacaa acattactaa gccatcagga cagtcaatta
     3241 gacaaagaag agcttccacg tatagaaaaa cagttgctac aagcaaataa gagaagagaa
     3301 aatgatgaag aaaagcttgt gagtctccgt tttgaattgg aggattgtga agctgcttta
     3361 gatgatttag ctgcttctct ggctaaagag ggacaaaaaa atgaaagcct tattcgtcag
     3421 caagctcaat tagagtcaca atgtgaacaa ttatctcaac aattgatggt attctcacgt
     3481 caattatcag aagactatca gatgacattg gatgaagcga aggtaaaagc aaatgtcttg
     3541 gaagatattt tgatggcgcg tgaacaattg aaatctctac aagctaaaat taaagcttta
     3601 ggacctgtta atattgatgc aattgctcaa tttgaagaag ttcatgaacg attgaccttc
     3661 cttaacacac agcgagacga cttagttcat gccaaaaatt tattactaga gacgattaca
     3721 gatatggatg atgaagttaa gactcgtttt aaatcgacat ttgaagccat tcgtcatagt
     3781 ttcaaagaaa cctttgtaca gatgtttggt ggaggttctg cggaccttat tctgacagaa
     3841 ggtgatttat tatcagctgg ggttgatatt tcggttcaac ctcctggtaa aaaaatccag
     3901 tctttaaact tgatgtcagg tggtgaaaaa gcattatcag cgttagcatt attatttgcc
     3961 attatccgtg ttaagacaat ttcatttgtc atcttggatg aagttgaggc cgctttagat
     4021 gaagcaaatg ttaagcgatt tggcgattat cttaatcgat ttgataaatc aagtcaattt
     4081 atcgttgtta ctcaccgaaa aggaactatg tctgctgctg atagtatcta tggggtaacg
     4141 atgcaggaat caggcgtttc caaaattgtt tctgtaaaat taaaagaagc tcaagaaatg
     4201 accaattaga aaacagaagg ggatcccttc tgttttttgg attttcagcc ttattttatg
     4261 ttacaatagt aaatgtaaac cctttagtta ggagattcca tgataaaatt agtagcaacc
     4321 gatatggatg gtacattctt agatgaaaac ggtacctatg ataaaaaacg tttagcaaac
     4381 gtgctaaaaa aatttaaaga acaaggaatt gtctttacgg cagcaagtgg gcgttcctta
     4441 ttatcacttg aacaattatt tgctgacttt cgtgatcaga tggcttttat agcagaaaac
     4501 ggaagtgcag cagtcttatt tgatcgcttg gcatatgaac agcacttgag tcgagaacaa
     4561 tacttagata ttattgacca tttgagtaag agcccttata tggagaataa tgaatatgtc
     4621 ttatctggaa aggatggtgc atatattcta agtgatgcca atccaggtta tattgaattc
     4681 atcactcatt attatgataa tttgcaaaaa gtttctcact ttgaagatgt tgatgatatt
     4741 attttcaaag taacggctaa ctttactgaa gagactgtta gacaagctga ggagtgggta
     4801 aatcaagtta taccgtatgc cacagcagtg acaacaggct ttaaatccat tgatattatt
     4861 ttaagctcag ttaataaacg aaatggttta gagcacctct gtgaacaata cggtattaga
     4921 gcagaagaag tcttatcttt tggtgacaat attaatgatt tagaaatgct agagtggtca
     4981 ggcaaagcta ttgcaacaga aaatgctaga ccagaagtta aagagatagc agattgtatt
     5041 ataggtcatc acaatgatca agctgtaatg gcttatttag aaagtatggt ggattaatgt
     5101 cggatattaa aatacttgct ttagatttag atggcacttt atttacaaca gataaaaaag
     5161 tttcagaaga aaataaagtg gccttaaaag cagctagaga aaaaggtata aaagttgtta
     5221 ttacaacagg gcgtccttta aaagcaattg gtaatttgtt agaggaccta gagttagtat
     5281 ctgatgaaga ttacagcatt acttttaacg gtggtttagt tcaacaaaat acagggaaaa
     5341 tcttagctaa aactgcaatg acacgacaag aagtggaaga tatccatgag gagttgtatc
     5401 aagtgggttt gccaacagat atcttaagtg agggaatggt atatagtatt gctaacaagg
     5461 gacaccattc tcaatatcat ttagccaacc cgctcttaga atttatagaa gttgatgatt
     5521 tagagcaagt gccaaaggat gtagtttata ataaaattgt tagtgttatt gatgctacat
     5581 atcttgatca acaaattgct aaacttcctg atagacttaa agttgactat gaaatgttta
     5641 aatcacgtga tatcatttta gaattgatgc ctaaaggtgt tcataaagca gtcggcttag
     5701 aactactgac aaaacacctt gggttagata gtagtcaagt tatggcaatg ggagatgaag
     5761 caaatgattt atcaatgtta gagtgggcag gcttaggagt tgcaatggct aatggtattc
     5821 cagaagctaa ggcaatagca aaagcgacaa ctatttgtaa taacgatgag tctggagttg
     5881 ccgaagcagt tgggaaatat attttaagtg aggaaaatta atgggattat ttgaccgcct
     5941 atttggacat aagaaaaaag ataaagaacc agaaattgaa gcatcagaat ctgttgtttt
     6001 agaagatgaa gattcagtta ttgataaaga ggagggcagc aacttttcga aggagtcaac
     6061 tttgaatcga acctctgaag tccctgttgc agaagatgat tctttcttag aattagaaag
     6121 agatacagca ttgtcagaat cacatcaacc agttacctct gaaattcatc ctttagagtc
     6181 agaagatacc gatgaaattc ctgtaaaaga agacgatagt tttttagaac tagaagatag
     6241 ggctaagaca aaggtagcag atacttctga ggtaggaaat gtagttcctg attccacgac
     6301 tctttctgac aatgtttctg ctaaatctga ggcatctttt agtgataagg agcaatcaag
     6361 taatagtcag gcttcagagc agttttcaga aactcccttt caagaagaga tgtctagtgg
     6421 aaaaactgag gtacaaacag agtcagagga tacatctgcg gctgatgctt ttttagcaga
     6481 ttactatgct aaacgtaaag caattgagaa agaaatctcg tctaattcgc tgtcaacaga
     6541 tgagtcaaaa ctttctgagg cacaggaagt tttatcgcaa tcacaggcag acactataaa
     6601 agctgagagt caagaggaaa aatataatcg tagcctgaaa aaaacacgca caggttttag
     6661 tgcacgcctt aatgctttcc tttctaactt ccgtcgtgtt gacgaagagt tctttgaaga
     6721 attagaagaa atgttaatct tatctgacgt tggggtaaat gttgcaacac agttaactga
     6781 agatcttcgt tatgaagcaa aattagagaa tgccaagaaa tcagaggatt taaaacgtgt
     6841 tatcgttgaa aaattagttg aaatctacga aaaagatggt atttataatg aggctatcaa
     6901 tttccaagaa ggtttaaccg taatgctttt tgtaggtgtt aatggtgttg gaaaaacgac
     6961 atctataggt aagttagctc atcaatacaa gtctcaaggg aaaaaagtta tgttagtagc
     7021 agctgatact ttccgtgcag gtgctgtggc tcaactagta gaatggggac gccgtgttga
     7081 tgtaccagtt gtcactggag aagaaaaagc agatccggct agcgttgtct ttgatggcat
     7141 ggaaaaagcc attgctcaag gcgttgatgt cttgttaatt gatacagcag gacgtttgca
     7201 aaataaagaa aaccttatgg cagagttgga aaaaattgga cgtattatta agcgtgtcgt
     7261 accagatgct ccccatgaaa ctttgcttgc tttagatgca tctacaggac aaaatgcttt
     7321 aagtcaagct aaagagttct cgaaaattac accattaact ggccttattc tgactaaaat
     7381 tgatggtact gctaagggtg gtgtggtact ggctattcgt caagaactag atattcctgt
     7441 taaattcatt ggtttcggcg aaaaaatcga tgatattggt gaatttaatt cagaagattt
     7501 catgcgtggc cttctggaag gaattttata agaaaaaaga actttcattt agaaagttct
     7561 ttttatatta aaagacacga taaacataag gattattagg tttagaaact tgacgagact
     7621 cttcaatgtg taagactggt aagttttgtt gtaacaattg attatactcg gatttaattg
     7681 tacctctaac agttacccaa gtattgtccg gataactttt ttgatttcca gttgttagta
     7741 gaccataaac tcctgaatcg gcaatacaat gaatgattcc aaagcggaaa ataaattgat
     7801 atccttcgtg tttgggttcg ttataaacaa acccagtata ttgtatttgg cgatccatga
     7861 attgctcggg atagagataa attagttcca taacttccat gtagttttca gttgtgatgg
     7921 ttaatgtacc agagccttta tattttttaa gctctttttg catttctttt tcataggctg
     7981 aagaagtaaa ataagtgctg gtatctggtt tcaaatattg aacgcgtgtc ccatcttgac
     8041 ttacagtacc agtagagccg gctgctagag ggaaattata tcccttggca gagactgtgg
     8101 ttgagtctaa actaactgta ggtactaaca aacctactaa aacgggaaat actaaaatca
     8161 ttgggcttgt tgatttagct attttaccat gtaggtgact atgcattttc atatttttaa
     8221 cccaaataat tagctgtaca attgctaaaa taaaagatag gaccattgaa atataggcca
     8281 aataggtata atgagtatta atatactgat ttagtttacc tgataatttt aaatacatgc
     8341 ttaattcaaa ataaccagca agaataagaa atcgaatcat tatattaccc ccacaaatag
     8401 acaatagatg ataataataa tagaagatac actaatgaat tggacaataa accttgtttt
     8461 aaaagaattt accatcatca ttaaattctt aatatcaatc atgggtccaa ttaaaaggaa
     8521 agccattact ggcgcgattc caaaggttga taataaagaa gcacctataa aagcgtcagc
     8581 ttcactacac aatgacaaaa tgaaagcaag caacatcata actaagatgg ctgtgatagg
     8641 gctgtgtccg atagtagtaa gaactcgagt tggtaaataa atttgcatag ctgaagcaat
     8701 taaagtacca aatactagat agcgtcctgt gtcaaaaaat tcatcgatgg catgagctaa
     8761 ggctagaaag attttttgat accacttctt atcagaataa tcatgaaaat gagtaggttt
     8821 agcgtcttct tttagaatat tatcatctac taaaaaggca agcatgactc ctaaagcaat
     8881 ggctacaatg gtagcaccta caaagcgtaa tatcaggaag cgtatagaat ttccaaatgc
     8941 tgagtaggtt gcaaataaaa caataggatt aataatagga gcagtagcaa gaaacggaac
     9001 tgccgtgtat gaaggcactt gcttttcaag gaatcgatta ataataggaa tgataccaca
     9061 ttcgcaagaa ggaaagacaa agccaacgaa ggttccaaat aagactctga gaaatttatt
     9121 tttcggtaag aacttattga ctatatcagg tgttataaag acttcaataa ttcctgataa
     9181 aatagttcca agcaaaacaa aaggaagtgc ctcaataata atggaaatga aaatggcaaa
     9241 ccattgaagg acactatccg gtaattgatt aaagatatcc atagaactta atctttctga
     9301 gaatcatctt tctgttcagg ttttgaatca gaatcaactt ttttaggttt tggtacttta
     9361 gcaaaactag cgaacatact ttctaagtca tc
//
LOCUS       DATRTN010000068         9372 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.068, whole genome shotgun sequence.
ACCESSION   DATRTN010000068 DATRTN010000000
VERSION     DATRTN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.068"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..1262
                     /locus_tag="VBE22_001534"
     CDS             <1..1262
                     /locus_tag="VBE22_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7202979.1"
                     /translation="RNGPVKEHGTPTKLYINSLKQKNYDIFNFGIDISGFRQVYNEEY
                     KKNQDGTFQKLKEEAFKLSDGEITELMRSFSSKPEYYTPIVTSADTSNNEILSKIQQQ
                     FETILTKENSIVNGTIEDPMGDKINLQLGNGQILQPSDYTLQGNDGSVMKDGIATGGP
                     NNDGGILKGVKLEYIGNKLYVRGLNLGEGQKVTLTYDVKLDDSFISNKFYDTNGRTTL
                     NPKSEDPNTLRDFPIPKIRDVREYPTITIKNEKKLGEIEFIKVDKDNNKLLLKGATFE
                     LQEFNEDYKLYLPIKNNNSKVVTGENGKISYKDLKDGKYQLIEAVSPEDYQKITNKPI
                     LTFEVVKGSIKNIIAVNKQISEYHEEGDKHLITNTHIPPKGIIPKTGGKGILSFILIG
                     GAMMSIAGGIYIWKRYKKSSDMSIKKD"
     gene            1392..3419
                     /locus_tag="VBE22_001535"
     CDS             1392..3419
                     /locus_tag="VBE22_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEO7202980.1"
                     /translation="MKKINKYFAVFSALLLTVTSLFSVAPVFAEEAKTTDTVTLHKIV
                     MPRTAFDGFTAGTKGKDNTDYVGKQIEDLKTYFGSGEAKEIAGAYFAFKNEAGTKYIT
                     ENGEEVDTLDTTDAKGCAVLKGLTTDNGFKFNTSKLTGTYQIVELKEKSTYNNDGSIL
                     ADSKAVPVKITLPLVNDNGVVKDAHVYPKNTETKPQVDKNFADKELDYANNKKDKGTV
                     SASVGDVKKYHVGTKILKGSDYKKLIWTDSMTKGLTFNNDIAVTLDGATLDATNYKLV
                     ADDQGFRLVLTDKGLEAVAKAAKTKDVEIKITYSATLNGSAVVEVLETNDVKLDYGNN
                     PTIENEPKEGIPVDKKITVNKTWAVDGNEVNKADETVDAVFTLQVKDGDKWVNVDSAK
                     ATAATSFKHTFENLDNAKTYRVIERVSGYAPEYVSFVNGVVTIKNNKDSNEPTPINPS
                     EPKVVTYGRKFVKTNKDGKERLAGATFLVKKDGKYLARKSGVATDAEKAAVDSTKSAL
                     DAAVKAYNDLTKEKQEGQDGKSALATVSEKQKAYNDAFVKANYSYEWVEDKNAKNVVK
                     LISNDKGQFEITGLTEGQYSLEETQAPTGYAKLSGDVSFNVNATSYSKGSAQDIEYTQ
                     GSKTKDAQQVINKKVTIPQTGGIGTIFFTIIGLSIMLGAVVIMKRRQSEEV"
     gene            3531..4409
                     /locus_tag="VBE22_001536"
     CDS             3531..4409
                     /locus_tag="VBE22_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO7202981.1"
                     /translation="MKTKKIIKKTKKKKSNLPFIILFLIGLSILLYPVVSRFYYTIES
                     NNQTQDFERAAKKLSQKEINRRMALAQAYNDSLNNVHLEDPYEKKRIQKGIAEYARML
                     EVSEKIGIISVPKIGQKLPIFAGSSQEVLSKGAGHLEGTSLPIGGNSTHTVITAHSGI
                     PDKELFSNLKKLKKGDKFYIQNIKETIAYQVDQIKVVTPDNFSDLLVVPGHDYATLLT
                     CTPIMVNTHRLLVRGHRIPYKGPIDEKLIKDGHLNTIYRYLFYISLVIIAWLLWLIKR
                     QRQKNRLSSVRKGIES"
     gene            4409..5293
                     /gene="srtC4"
                     /locus_tag="VBE22_001537"
     CDS             4409..5293
                     /gene="srtC4"
                     /locus_tag="VBE22_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2a pilus assembly sortase SrtC4"
                     /protein_id="HEO7202982.1"
                     /translation="MRGKFQKNLKKSVVLNRWMNIGLILLFLVGLLITSYPFISNWYY
                     NIKANNQVTNFDNQTQKLNAKEINRRFELAKAYNRTLDPSRLSDPYTEKEKKGIAEYA
                     HMLEITEMIGYIDIPSIKQKLPIYAGTTSSVLEKGAGHLEGTSLPIGGKSSHTVITAH
                     RGLPKAKLFTDLDKLKKGKIFYIHNIKEVLAYKVDQISVVKPDNFSKLLVVKGKDYAT
                     LLTCTPYSINSHRLLVRGHRIKYVPPVKEKNYLMKELQTHYKLYFLLSILVILILVAL
                     LLYLKRKFKERKRKGNQK"
     gene            5290..6216
                     /gene="pilC"
                     /locus_tag="VBE22_001538"
     CDS             5290..6216
                     /gene="pilC"
                     /locus_tag="VBE22_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2a pilus subunit PilC"
                     /protein_id="HEO7202983.1"
                     /translation="MKKIRKSLGLLLCCFLGLVQLAFFSVASVNADTPNQLTITQIGL
                     QPNTTEEGISYRLWTVTDNLKVDLLSQMTDSELNQKYKSILTSPTDTNGQTKIALPNG
                     SYFGRAYKADQSVSTIVPFYIELPDDKLSNQLQINPKRKVETGRLKLIKYTKEGKIKK
                     RLSGVIFVLYDNQNQPVRFKNGRFTTDQDGITSLVTDDKGEIEVEGLLPGKYIFREVK
                     ALTGYRISMKDAVVAVVANKTQEVEVENEKETPPPTNPKPSQPLFPQSFLPKTGMIIG
                     GGLTILGCIILGILFIFLRKTKNSKSERNDTV"
     regulatory      6303..6448
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6518..7102
                     /locus_tag="VBE22_001539"
     CDS             6518..7102
                     /locus_tag="VBE22_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO7202984.1"
                     /translation="MTNTRKMVTIAILSALSFVLMMVSFPLIPGAEFLKVDFSILPML
                     VTFILFDLKSSYGVLLLRSLLKVILANRGPETFIGLPMNMVALALFLASFAIFWKNRE
                     SAKDFIKASLFGTVSLTVSMVALNYVFAIPLYAIFANFDIRTFIGVGNYLLTMVIPFN
                     IVEGILISIVFYLTYVACLPILERYKKTNVISSK"
     gene            7083..7724
                     /locus_tag="VBE22_001540"
     CDS             7083..7724
                     /locus_tag="VBE22_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO7202985.1"
                     /translation="MLSRQNSKLIQAFIAIILFFSLGLVIKYWPDTVIAFDQTIQESV
                     RGQLPNLSTRFFKLITVIGNTVSQIAIAIMSVTFCYLKKWYPQARFIAVNAIISGICI
                     LSLKLIFQRVRPTLPHLVFAGGYSFPSGHSMGTFMIFGSIIILLQYYMPNSIWKLLCQ
                     GILGLLIFLIGLSRIYLGVHFPTDVLAGFILAYGILNLTAYIFLAKDWLKKVK"
     gene            7817..8752
                     /locus_tag="VBE22_001541"
     CDS             7817..8752
                     /locus_tag="VBE22_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747785.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEO7202986.1"
                     /translation="MKKRYRAINDYYRELFGEKIFKLPIDAGFDCPNRDGTVARGGCT
                     FCTVSGSGDAIVAPEAPIREQFYKEIDFMHRKWPEVNKYLVYFQNFTNTHAKLEIIKE
                     RYEQAINEPGVIGINIGTRPDCLPDETIYYLAELSERMHVTLELGLQTTYEATSALIN
                     RAHSYDLYKKTVKRVRELAPKVEIVSHLINGLPGETHDMMVENVRRCVMDNDIQGIKL
                     HLLHLMTNTRMQRDYHEGRLRLLSQEDYISIICDQLEIIPKHIVIHRITGDAPRHMLI
                     GPMWSLNKWEVLNAIDKEMEKRQSYQGCKVEVKHD"
     gene            8745..9311
                     /locus_tag="VBE22_001542"
     CDS             8745..9311
                     /locus_tag="VBE22_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000596040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO7202987.1"
                     /translation="MIKRPIHLSHDFLAEVIDKEAITLDATMGNGNDTVFLAKSSKKV
                     YAFDIQEEAIAKTKAKLTGQGISNVELILDGHENLEQYVHTPLRAAIFNLGYLPSADK
                     TVITKPHTTIKAIKNVLDILEVGGRLSLMVYYGHDGGKSEKDAVIAFVEQLPQNNFAT
                     MLYQPLNQVNTPPFLIMVEKLQSYENEV"
BASE COUNT         3321 a         1442 c         1739 g         2870 t
ORIGIN      
        1 atcgaaatgg accagtgaaa gaacatggaa caccaaccaa actttatata aatagtttaa
       61 aacagaaaaa ttatgacatt tttaattttg gtatcgatat atctggtttt agacaagttt
      121 ataatgagga gtataagaaa aatcaagatg gtacttttca aaaattgaaa gaggaagctt
      181 ttaaactttc agatggagaa atcacagaac taatgaggtc gttctcttcc aaacctgagt
      241 actacacccc tatcgtaact tcagccgata catctaacaa tgaaatttta tctaaaattc
      301 agcaacaatt tgaaacgatt ttaacaaaag aaaactcaat tgttaatgga actatcgaag
      361 atcctatggg tgataaaatc aatttacagc ttggtaatgg acaaatatta cagccaagtg
      421 attatacttt acagggaaat gatggaagtg taatgaagga tggtattgca actggtgggc
      481 ctaataatga tggtggaata cttaaggggg ttaaattaga atacatcgga aataaactct
      541 atgttagagg tttgaattta ggagaaggtc aaaaagtaac actcacatat gatgtgaaac
      601 tagatgacag ttttataagt aacaaattct atgacactaa tggtagaaca acattgaatc
      661 ctaagtcaga ggatcctaat acacttagag attttccaat ccctaaaatt cgtgatgtga
      721 gagaatatcc tacaataacg attaaaaacg agaagaagtt aggtgaaatt gaatttataa
      781 aagttgataa agataataat aagttgctcc tcaaaggagc tacgtttgaa cttcaagaat
      841 ttaatgaaga ttataaactt tatttaccaa taaaaaataa taattcaaaa gtagtgacgg
      901 gagaaaacgg caaaatttct tacaaagatt tgaaagatgg caaatatcag ttaatagaag
      961 cagtttcgcc ggaggattat caaaaaatta ctaataaacc aattttaact tttgaagtgg
     1021 ttaaaggatc gataaaaaat ataatagctg ttaataaaca gatttctgaa tatcatgagg
     1081 aaggtgacaa gcatttaatt accaacacgc atattccacc aaaaggaatt attcctaaga
     1141 caggtgggaa aggaattcta tctttcattt taataggtgg agctatgatg tctattgcag
     1201 gtggaattta tatttggaaa aggtataaga aatctagtga tatgtccatc aaaaaagatt
     1261 aaaaattatg cgctttagta ttcttaatta attaactaaa attccatgac ttgaagggag
     1321 tggtgctgcg gtgtattaaa atccgcatat tattaatcat gttgattaac taaaataaga
     1381 aggagataga aatgaaaaaa atcaacaaat attttgcagt cttctcggca ttgctactga
     1441 ccgtaacatc attgttctca gttgcaccag tgtttgcgga agaagcaaaa actactgaca
     1501 cagtgacctt gcacaagatt gtcatgcctc gaactgcatt tgacggtttt actgctggta
     1561 caaagggtaa ggataatact gactacgttg gtaaacaaat cgaagacctt aaaacttact
     1621 ttggctcagg cgaagcgaaa gaaatcgcag gtgcttactt tgctttcaaa aatgaagctg
     1681 gtactaaata catcactgaa aatggtgaag aagttgatac tttggataca acagatgcca
     1741 aaggttgtgc tgttcttaaa ggtttaacaa cagacaatgg tttcaaattt aacacttcta
     1801 aattaacagg aacttaccaa atcgttgaat tgaaagaaaa atctacatac aacaacgatg
     1861 gttctatctt ggctgattca aaagcagttc cagttaaaat cactcttcca ttggtaaacg
     1921 acaatggtgt tgttaaagac gctcacgttt atccaaagaa cactgaaaca aaaccacaag
     1981 tagataagaa cttcgcagat aaagaacttg attatgcgaa caacaaaaaa gacaaaggga
     2041 ctgtctcagc atctgttggt gatgttaaaa aataccatgt tggaacaaaa atccttaaag
     2101 gttcagacta taagaaatta atctggaccg atagcatgac caaaggtttg actttcaaca
     2161 acgatattgc tgtaacattg gatggtgcaa ctcttgatgc tacaaattac aaacttgtag
     2221 cagatgacca aggtttccgc cttgtcttga ctgacaaagg tcttgaagca gtggcaaaag
     2281 ccgcaaaaac aaaagatgtt gaaatcaaga tcacttactc agctactttg aacggttctg
     2341 ctgtcgttga agttctagaa accaatgatg ttaaattgga ctacggcaac aacccaacaa
     2401 ttgaaaatga accaaaagaa ggtattccag ttgataagaa aatcactgtt aacaaaacat
     2461 gggcagtaga tggcaatgaa gtgaataaag cagatgaaac agttgatgct gtcttcacct
     2521 tgcaagttaa agatggtgac aaatgggtga atgttgattc agctaaagca acagctgcaa
     2581 ctagcttcaa acacactttt gaaaacttgg ataatgctaa aacttaccgc gttatcgaac
     2641 gtgttagcgg ctacgctcca gaatacgtct catttgtaaa tggcgttgta accatcaaga
     2701 acaacaaaga ctcaaatgag ccaactccaa tcaacccatc agaaccaaaa gtggtgactt
     2761 atggacgtaa atttgtgaaa acaaataaag atggaaaaga acgcttggca ggagctacct
     2821 tccttgttaa gaaagatggc aagtacttgg cacgtaaatc aggtgttgca acagatgcag
     2881 aaaaagctgc tgtagattca actaaatcag cattggatgc tgctgttaaa gcttacaatg
     2941 atttgactaa agaaaaacaa gaaggtcaag atggtaaatc agcattggct accgttagtg
     3001 aaaaacaaaa agcttacaat gatgcctttg ttaaagctaa ctactcatac gaatgggttg
     3061 aagataaaaa tgctaagaat gttgttaaat tgatttctaa cgataaaggt caatttgaaa
     3121 ttactggctt gactgaaggt caatactcat tggaagaaac acaagcacca actggttatg
     3181 ctaaattatc aggtgatgtt tcgtttaatg ttaatgctac ttcatacagt aaaggttctg
     3241 ctcaagatat tgagtatacc caaggttcta aaactaaaga tgcacaacaa gttatcaata
     3301 agaaggttac tattccacaa acaggtggta ttggtacaat ttttttcaca attattggat
     3361 taagtattat gcttggagcg gtagttatca tgaaaagacg tcaatcagag gaagtttaag
     3421 attagctttt gatgatgtgt aagcacagtt aaagctgtgc ttatgatcta agggtatttc
     3481 agtagaagta ctcttagatc ataagcaaga gccattattt aggagatgac gtgaagacta
     3541 aaaaaattat caaaaaaaca aaaaaaaaga aatcaaatct gccttttatc attctttttc
     3601 taataggtct atctatctta ttgtatccag tggtatcacg attttactat acgatagaat
     3661 ctaataacca aacacaagat tttgagagag ctgctaaaaa acttagtcag aaagaaatca
     3721 atcggcgtat ggctctagca caagcatata atgattcttt aaataatgtc catcttgaag
     3781 atccttatga gaaaaaacga attcaaaagg ggatagcaga gtacgcccgt atgttagagg
     3841 taagtgaaaa aatcggcata atttcagttc ctaagatagg tcaaaaactc cctatatttg
     3901 caggttcaag tcaagaagtt ctatctaaag gagcaggtca tttagaaggt acctctcttc
     3961 caattggagg caatagcacc cataccgtta taacagcgca ctcaggaatt ccagataaag
     4021 agctcttttc taaccttaaa aagttaaaaa aaggagataa gttttatatt caaaacataa
     4081 aagaaacgat agcatatcaa gtagatcaga taaaagtcgt tacacccgat aacttttcag
     4141 atttgttggt tgttcctgga catgattatg caaccttatt gacttgcacc ccgattatgg
     4201 tcaatacaca cagactttta gtaaggggtc atcgtatccc ttataaaggt cctattgatg
     4261 aaaaattaat aaaagacggt catttaaaca cgatttatag atatcttttc tatatatctt
     4321 tagttattat tgcttggtta ctttggttaa taaaacgtca acgtcaaaaa aatcgtttat
     4381 caagtgttag aaaaggaatt gaatcataat gcgaggaaaa tttcagaaaa accttaagaa
     4441 atcggtcgtt ttaaatcgat ggatgaatat aggtttgata ttattattct tagtaggtct
     4501 tttgataacc tcatatcctt ttatttcaaa ttggtactat aatattaaag ctaataatca
     4561 agtaactaac tttgataatc aaacccaaaa attaaatgct aaagagatta atagacgatt
     4621 tgagttagca aaagcttata atagaacact ggacccaagc cgcctatcag atccctatac
     4681 tgaaaaagaa aaaaaaggta ttgctgaata cgcccatatg cttgagatta ctgaaatgat
     4741 tggatatatt gatataccgt ctatcaagca aaaattacct atctatgcgg ggactaccag
     4801 tagtgttctt gaaaaaggag caggacacct tgaaggaact tctttgccaa ttggtggaaa
     4861 aagttcacat actgttatca cagctcatcg cggcttacct aaagctaagt tatttacaga
     4921 tttagataaa cttaaaaaag gaaaaatttt ttatattcat aatatcaaag aagttttagc
     4981 ctataaggtt gatcaaataa gtgttgtaaa gccagataat ttttctaaat tattggttgt
     5041 taaaggtaag gattatgcga ctttgctaac atgtacacct tattcgatta attcacatcg
     5101 tttactagtt agagggcatc gaatcaagta tgtacctcct gttaaagaaa agaactattt
     5161 aatgaaagaa ttgcaaacac actataaact ttatttcctc ttatcaatcc tagttattct
     5221 tatattagtc gctttactat tatatttaaa acgaaaattt aaagagagaa agagaaaggg
     5281 aaatcaaaaa tgaaaaagat tagaaaaagt ttaggacttc tactatgttg ctttttagga
     5341 ttggtacaat tagcgttttt ttcggtagcc agtgtaaatg ctgatacccc taatcaacta
     5401 acaatcacac agataggact tcagccaaat actacagagg aggggatttc ttatcgttta
     5461 tggactgtga ctgacaactt aaaagttgat ttattgagcc aaatgacaga tagcgaattg
     5521 aaccagaagt ataagagtat cttgacttct cctactgata ctaatggtca gacaaagata
     5581 gcactcccaa atggttcgta ctttggtcgt gcttataaag ctgatcaaag cgtttcaaca
     5641 atagtacctt tttatattga attaccagat gataagttat caaatcaatt acagataaat
     5701 cctaagcgaa aagttgaaac aggccgatta aaacttatta aatatacaaa agaaggaaag
     5761 ataaagaaaa ggctatcagg agtaatattt gtattatacg ataaccagaa tcagccagtt
     5821 cgctttaaaa atggacgatt tacgaccgat caagatggga ttacttcatt agtaactgat
     5881 gataagggag aaattgaggt tgaaggttta ttacctggta agtatatttt tcgagaagta
     5941 aaagcactaa ctggttaccg tatatctatg aaggatgctg tagttgctgt agttgctaat
     6001 aaaacacagg aagtagaggt agaaaacgaa aaagaaactc ctccaccaac aaatcctaaa
     6061 ccatcacaac cgctttttcc acaatcattt cttcctaaaa caggaatgat tattggtgga
     6121 ggactgacaa ttcttggttg tattattttg ggaattttgt ttatcttttt aagaaaaact
     6181 aaaaatagca aatctgaaag aaacgataca gtatagtaat ttggtattaa tatgattttt
     6241 aatcactact actaattagt aattaaaaaa cttgcaattg caagtttttt ttgttatgct
     6301 ataggtgtct tcagggcagg gtgtaattcc cgaccggcgg taatcgcttc caacttattt
     6361 gtcagttgtg aagcatcagt ccgcgagcga aagctgatgt ggtgagattc cacaaccgac
     6421 agtaaagtct ggatgggaga agacaaaggt ttttttcgcg tacctacgct tacctttgga
     6481 gcttctttca atttatttaa attggaggaa tttttttatg acaaatacac gtaaaatggt
     6541 gacaatcgcc attctttctg ccttatcttt tgtattgatg atggtttcat ttccattaat
     6601 accaggagca gaatttttaa aggttgattt ttcaatcctt ccgatgttag ttacttttat
     6661 tttatttgat ttgaaatcaa gttatggtgt actcttatta agatcacttt taaaagttat
     6721 acttgctaat cgtggtccag aaacctttat cggtttaccg atgaatatgg ttgctctagc
     6781 tttattttta gcaagtttcg ctattttctg gaaaaataga gagagtgcaa aagattttat
     6841 taaagctagt ctatttggta cagttagctt gactgtatct atggtagctc tcaactatgt
     6901 ctttgcgatt cctttgtatg ctatatttgc taactttgat atcagaacat ttattggtgt
     6961 aggcaattac cttttaacaa tggttattcc atttaatatc gttgagggaa ttttaatttc
     7021 gattgttttc tatctaactt acgtggcttg tttgccaata ttagaaagat ataaaaagac
     7081 taatgttatc tcgtcaaaat agtaaactta ttcaagcatt tattgctatt atcttatttt
     7141 tcagtttagg attggtcatc aaatattggc ccgacactgt aattgcattt gaccaaacta
     7201 ttcaagaaag tgttagagga cagttgccaa atctttctac aagatttttc aagttaatca
     7261 ctgtgattgg taatactgta agccagatag ctattgcgat tatgagcgtc actttctgtt
     7321 accttaagaa gtggtatcct caagctagat ttatcgcagt aaatgctatt atctctggta
     7381 tatgtatact ttctttaaaa cttatttttc aaagagtaag accaacttta ccccatttag
     7441 tatttgcagg tggctattcg tttcccagtg ggcactcgat gggaacattt atgatatttg
     7501 ggagtattat tattttattg cagtactata tgcctaatag tatatggaaa ctgttgtgcc
     7561 aaggtatttt gggtttgctc atttttttaa ttgggttatc tcgtatttac ctaggggttc
     7621 attttccgac agatgtttta gctggtttta tacttgctta tggtattttg aatctaactg
     7681 cgtatatctt tttagccaag gattggttaa agaaagtgaa gtaaataaaa gattgggact
     7741 aactgatgtt ccaatctttt ttagttttat tgtagtagtg aggttatttt agttaaaatt
     7801 gtgatacaat tatcctatga aaaaacgtta tcgtgcgatt aatgattatt atcgagaact
     7861 ctttggagaa aaaatattta aattacctat agatgctggt tttgactgtc ctaacagaga
     7921 cggaacagtt gctagaggag ggtgcacttt ttgcacagtt tctggttcag gggatgctat
     7981 agttgctcca gaagctccta ttcgtgaaca attctataaa gaaattgatt tcatgcatag
     8041 aaagtggcct gaagttaata aatacttagt ctattttcaa aattttacca atacccatgc
     8101 taagttagag ataattaaag agcgttacga acaggctatt aatgagccag gagttatcgg
     8161 gatcaacatc ggaacaaggc cggattgctt acctgatgaa actatatact atctcgctga
     8221 actttctgaa agaatgcatg tgactttaga attaggcctt cagacaactt atgaagcaac
     8281 ttctgcatta ataaatagag cacattcata tgacctttac aaaaaaactg ttaagagagt
     8341 acgagaatta gctcctaaag tagaaatcgt ttctcaccta ataaatggtc tcccagggga
     8401 gacacatgat atgatggtgg aaaatgttcg tcgatgtgtt atggataatg atattcaggg
     8461 tataaaatta caccttctgc atcttatgac taatacaaga atgcagagag attaccatga
     8521 aggtcgttta cgtttgttaa gtcaagaaga ctatatttcg attatttgtg accaactgga
     8581 gattattcca aaacatattg ttattcaccg aattactggt gatgcgccac gtcatatgtt
     8641 aattggaccg atgtggagcc ttaataaatg ggaagtgctt aatgccattg acaaagaaat
     8701 ggagaaaaga cagtcatatc aaggatgtaa agttgaggta aaacatgatt aaaagaccaa
     8761 ttcatctttc tcatgatttt ttagcagaag ttattgataa agaagcaata acgcttgatg
     8821 caactatggg aaatgggaat gatactgttt ttttagcgaa aagttctaaa aaagtttatg
     8881 catttgatat tcaagaagaa gcaatagcaa aaacgaaagc aaagctcacg gggcaaggga
     8941 tctctaacgt tgaacttatc ttggatgggc atgaaaattt ggaacagtat gttcatacac
     9001 ctttaagagc agctatattt aatctgggat acttaccctc agcggataaa acagttatta
     9061 caaaacctca cacaactatc aaggcaatca aaaacgtttt agatatttta gaagtaggtg
     9121 gacgtctatc gcttatggtt tattatggtc atgatggtgg aaaaagtgag aaagatgcgg
     9181 tgatagcatt cgtggaacaa ttaccacaga ataatttcgc tacaatgctt tatcaaccac
     9241 tcaatcaagt taatacgccc ccctttttaa ttatggtaga aaagttacaa tcatatgaaa
     9301 atgaggtgta actatcttta ggcaattagc aaaaatgata attgctataa aatgccataa
     9361 acaatattat cg
//
LOCUS       DATRTN010000069         9293 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.069, whole genome shotgun sequence.
ACCESSION   DATRTN010000069 DATRTN010000000
VERSION     DATRTN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.069"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..502
                     /locus_tag="VBE22_001543"
     CDS             <1..502
                     /locus_tag="VBE22_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEO7202988.1"
                     /translation="SVVKRVTDTIEMQKKLNTIQNLLENTPSYQKPITTSNLTKIRSI
                     LSYLGITSETAYTDILNICELLLKQELNFAQFDFQKELSIDEHQQKIILQRIRRAVKK
                     AMINMAHLYIDDFENELTLQYANALFGFQNIHNEAQLIQGKSMYGGKISLKHFFDELI
                     LQSKTF"
     gene            724..1446
                     /locus_tag="VBE22_001544"
     CDS             724..1446
                     /locus_tag="VBE22_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7202989.1"
                     /translation="MISFENVSKSYGDHTIIDNISCHIQRGEFFVLVGASGSGKTTIL
                     KMINRLIEPSQGAITLDGENITSLDLRQLRLETGYVLQQIALFPNLTVGENIELIPEM
                     KGWSKGDQKKAASDLLDKVGLPAKDYFNRYPHELSGGEQQRIGILRAIVAKPKVLLMD
                     EPFSALDPISRRQLQDITKQLQSELGITLVFVTHDMKEAMRLADRICVIKEGKIVQLD
                     RPEIIQNNPSDQFVRTLFEEEN"
     gene            1449..2963
                     /locus_tag="VBE22_001545"
     CDS             1449..2963
                     /locus_tag="VBE22_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019319700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEO7202990.1"
                     /translation="MTTLITTFQERFGDWTQSLIEHLQLSLLTLILATLIAIPLGIII
                     SHYKKISHVVLQITGIFQTIPSLALLGLFIPFMGIGTVPAVVALIIYALFPILQNTVT
                     VLMQIDANLIEAATAFGMTRWERLKKFELALSMPVIISGIRTASVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNPSLILIGAISSAVLAIIFSGLIGLLEKARLRTIAVSGILLLA
                     GLGLSYAPKWMPGTNTATITVAGKLGTEPDILINMYKELIEDQTDIKVKLKPNFGKTT
                     FLYQALKSGDIDLYPEFTGTITSSLLKNPPKVSNNPKQVYNLAKNGILKQDKLSLLSP
                     MAYQNTYAVAVKKDYAEANQLKNISDLKKLDKLKAGFTLEFKDREDGSIGLQKHYGLN
                     LDISTLEPALRYQAINSKDVNIIDAYSTDSELIQYQLQILKDDKHLFPPYQGAPLLRQ
                     DTIKKYPQVKKALNKLAGHITEKEMQEMNYQVAVKHKSAATVAKQYLKAHHIIK"
     gene            complement(3042..5582)
                     /gene="mprF"
                     /locus_tag="VBE22_001546"
     CDS             complement(3042..5582)
                     /gene="mprF"
                     /locus_tag="VBE22_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEO7202991.1"
                     /translation="MKKLIEKVKSLTSVIKIVFFISVLVLIIVEMIHLKRTISVEQLK
                     SVFGQLSPMNLFLIILVGVIAVLPTTGYDFVLNGLLRTDKSKRYILQTSWCINTFNNL
                     SGFGGLIDIGLRMAFYGKKGQEKSDLREVTRFLPYLISGLSFISVIALIMSHIFHAKA
                     SVDYYYLVLIGASMYFPVIYWISGHKGSHYFGDMPSSTRIKLGVVSFFEWGCAAAAFI
                     IIGYLMGIHLPVYKILPLFCIGCAVGIVSLIPGGLGSFELVLFTGFAAEGLPKETVVA
                     WLLLYRLAYYIIPFFAGIYFFIHYLGSQINQRYENVPKELVSTVLQTMVSHLMRILGA
                     FLIFSTAFFENITYIMWLQKLGLDPLQEQMLWQFPGLLLGVCFILLARTIDQKVKNAF
                     PIAIIWITLTLFYLNLGHISWRLSFWFILLLLGLLVIKPTLYKKQFIYSWEERIKDGI
                     IIVSLMGVLFYIAGLLFPIRAHITGGSIERLHYIIAWEPIALATLILTLVYLCLVKIL
                     QGKSCQIGDVFNVDRYKKLLQAYGGSSDSGLAFLNDKRLYWYQKNGEDCVAFQFVIVN
                     NKCLIMGEPAGDDTYIREAIESFIDDADKLDYDLVFYSIGQKLTLLLHEYGFDFMKVG
                     EDALVNLETFTLKGNKYKPFRNALNRVEKDGFYFEVVQSPHSQELLNSLEEISNTWLE
                     GRPEKGFSLGYFNKDYFQQAPIALVKNAEHEVVAFANIMPNYEKSIISIDLMRHDKQK
                     IPNGVMDFLFLSLFSYYQEKGYHYFDLGMAPLSGVGRVETSFAKERMAYLVYHFGSHF
                     YSFNGLHKYKKKFTPLWSERYISCSRSSWLICAICALLMEDSKIKIVK"
     gene            complement(5566..6309)
                     /locus_tag="VBE22_001547"
     CDS             complement(5566..6309)
                     /locus_tag="VBE22_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7202992.1"
                     /translation="MTENWLHTKDGSDIYYRVVGQGQPIVFLHGNSLSSRYFDKQIAY
                     FSKYYQVIVMDSRGHGKSHAKLNTISFRQIAVDLKDILVHLEIDKVILVGHSDGANLA
                     LVFQTMFPGMVRGLLLNSGNLTIHGQRWWDILLVRIAYKFLHYLGKLFPYMRQKAQVI
                     SLMLEDLKISPADLQHVSTPVMVLVGNKDIIKLNHSKKLASYFPRGEFYSLVGFGHHI
                     IKQDSHVFNIIAKKFINDTLKGEIVEKAN"
     gene            complement(6443..6799)
                     /locus_tag="VBE22_001548"
     CDS             complement(6443..6799)
                     /locus_tag="VBE22_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEO7202993.1"
                     /translation="MSTLTIIIATLTALEHFYIMYLETLATQSNMTGKIFSMSKEELS
                     YLPVIKLFKNQGVYNGLIGLFLLYGLYISQNQEIVAVFLINVLLVAIYGALTVDKKIL
                     LKQGGLPILALLTFLF"
     gene            complement(6866..9184)
                     /locus_tag="VBE22_001549"
     CDS             complement(6866..9184)
                     /locus_tag="VBE22_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEO7202994.1"
                     /translation="MLDELKAIIKSPKLWITMAGVALIPTLYNVIFLSSMWDPYGNTK
                     NLPVAVVNQDKSAKLNGKTISIGKDMEDNLSKNDSLDFHFTTAKRAEKELEKGHYYMV
                     ITFPKDLSRKATTLMTEKPERLNITYKTTKGRSFVASKMSETAANKLKDEVAESITGT
                     YTESVFKNMGSMKTGINKAADGSQELLNGSNKLQDGSQTLTSNLDVLASSSQTFSGGA
                     NKLNSGINLYTDGVGTLSNGLETLSDGVTAYTTGVHKLSEGSQKLDDKSQALVEGSEK
                     LTDGLQQLSQATQLKPEQERTLQNLSDGLKNLNQIITNLQSTATTDSDTNSKLFNFLS
                     TIESSTKALMNTAAADKQKQMTAVQSTSAFKSLTPEQQSQITSAVTGTPTSAETIAAN
                     ISSNIENMKTVLSEASSSAPSNNGSQNLQTLSGTANNLVLKAISDLDKIQKLPTATKQ
                     LYQGSQTLTKGITDYTNAVGQLRKGAVTLDSKSNQLISGTQKASQGAQTLDSKSDQLR
                     DGAGQLASGSDRIADGSNKLAGGGHQLTDGLTELSGGVSQLSSSLGKAGDQLSMVSVN
                     KDNANAVSSPVTIKHEDYDSVDTNGVGMAPYMISVALMVVALSANVIFAKALSGKEPA
                     NRFSWAKNKLLINGFIATLAATILFFAVQFIGLKPDYPGKTYFIILLTAWTLMALVTA
                     LVGWDNRYGSFLSLLILLFQLGSSAGTYPIELSPKFFQTIQPFLPMTYSVSGLRETIS
                     LTGDVNHQWRMLVIFLVSSMILALLIYRKQED"
BASE COUNT         3172 a         1783 c         1423 g         2915 t
ORIGIN      
        1 aagcgtcgtg aaacgtgtca ctgatactat cgaaatgcaa aaaaaattaa atacaatcca
       61 aaatctccta gaaaatacgc catcatatca aaagcctata acgactagta acctcactaa
      121 aatccgttca atactcagtt atttaggtat tacttcagag acagcctaca cagatattct
      181 taacatctgt gaacttttat taaaacaaga attaaatttt gcccaatttg attttcaaaa
      241 agaactttct atcgatgaac accaacaaaa aatcatttta caacgtattc gtcgtgccgt
      301 taaaaaagct atgataaata tggctcatct gtacattgat gattttgaaa atgaattaac
      361 actgcaatat gctaatgcat tgtttggatt tcagaatatc cataatgagg cacaacttat
      421 tcaaggaaaa agcatgtatg gtggtaaaat ttcacttaaa cattttttcg atgaacttat
      481 tttacagagt aaaacgtttt aagacttgtt taagcatggc cttatatact ataaccatcc
      541 taaaactttt cttttcataa atctccaaca aacaccttgt ctaccgcaag gtgtttgttt
      601 ttgcttctag tttatcgttt ctgacaattt aaatgtcaga tagttgtttg aatgtctact
      661 ttatgctaat ataaccctaa tatcatacga cctacactcg ttacgcacta gaaagtaaat
      721 tacatgattt catttgaaaa tgttagtaaa tcatatggtg atcataccat cattgataat
      781 attagttgcc atattcaaag aggagaattc tttgtccttg tgggggccag tggcagtggt
      841 aaaactacca tcctcaagat gattaatcgt ctcatcgaac caagccaagg cgctattact
      901 ttagatgggg aaaacattac atcacttgat ttgcgacaat tacgcttaga aactggttat
      961 gtcttgcaac aaattgcact tttccctaat ttaacagttg gagaaaatat agaacttatc
     1021 cctgaaatga agggctggtc aaaaggtgac caaaaaaaag ccgctagcga ccttcttgat
     1081 aaggtgggtt tacctgctaa ggactacttt aatcgctatc ctcatgagct ctctggtggg
     1141 gaacaacaac gcataggtat cctacgagca atcgttgcta aacctaaggt actacttatg
     1201 gatgagccat tttctgcttt agaccctatc tcaagacgtc aattacaaga tatcaccaaa
     1261 caattacagt ctgaattggg tattacatta gtgtttgtca cccatgacat gaaggaagct
     1321 atgcgtcttg cagatagaat ttgtgttatt aaggaaggta agatagtgca attggataga
     1381 ccagaaataa ttcaaaataa tccctctgac caatttgtta gaaccctatt tgaagaggag
     1441 aactgagtat gacaacacta atcactacct ttcaagagcg ttttggagat tggactcaaa
     1501 gtcttattga acacctccag ctatcactac taacacttat tttagcaact ttaattgcta
     1561 ttccactcgg tatcatcatt agtcattata aaaaaatcag tcatgttgtc cttcagataa
     1621 ctgggatatt tcaaacaatt ccttctttag ccttgcttgg tttatttata ccttttatgg
     1681 gaattggaac tgtaccagct gtcgttgcac taattattta cgctctattt ccaattttac
     1741 aaaatactgt aacagtactg atgcaaattg atgctaatct cattgaggca gcaaccgctt
     1801 ttggaatgac acgttgggaa aggcttaaaa agtttgagtt agctttaagt atgcctgtta
     1861 tcatctcagg cattagaact gcctctgtta tgattattgg tacagctaca ttagctagtc
     1921 ttatcggagc tggtggcctt ggatcattta ttttgttagg gattgatcgc aacaatcctt
     1981 ctctaattct cataggggca atctcttctg ctgtcttagc tattatattt agtggtttaa
     2041 ttggattact agaaaaggct agattacgaa ccatagccgt ttctggtata cttttactag
     2101 caggcttagg actatcctat gctccaaaat ggatgcctgg aacaaatact gcaacgataa
     2161 ctgttgcagg gaagttagga actgaaccag atattttaat taatatgtac aaagaactta
     2221 ttgaggatca gacagatatc aaagttaagc ttaaacctaa tttcggaaaa acaactttct
     2281 tatatcaagc cctaaaatct ggtgatattg atctttaccc agagtttaca ggtactatta
     2341 catcaagttt acttaaaaat cctcctaaag taagtaataa ccctaaacag gtttacaact
     2401 tagccaaaaa cggtatttta aaacaagata agttgtcact tttgtcacct atggcttatc
     2461 aaaacaccta cgctgtagct gttaaaaaag attacgctga agctaatcaa ctcaaaaata
     2521 tcagcgacct aaaaaaatta gataaactaa aagctggttt cactttagaa tttaaggata
     2581 gagaggatgg gagtattggt ttacagaaac attatggttt aaaccttgat atctcaacct
     2641 tagaacctgc tttacgatac caagctatca atagtaaaga tgtcaatatt attgacgctt
     2701 actctaccga tagtgaatta atccagtatc aacttcaaat tctcaaagac gataagcatc
     2761 tatttccgcc atatcaagga gcaccgctac taaggcaaga tacgattaaa aaataccctc
     2821 aagtaaaaaa agctctcaat aaacttgctg gtcatatcac tgaaaaagaa atgcaagaaa
     2881 tgaactacca agtagctgta aagcataaat cagcagctac agtagctaaa caatatttaa
     2941 aagcacatca tattataaag tagattcaag caatcaatgg tattaaatga tttaaaaaga
     3001 gcatgtcgca tgacatgctc tttttaattg ccaaataaag cttatttaac aatcttaatt
     3061 ttactatctt ccattaatag ggcacaaata gcacaaatta accaggacga acgagaacaa
     3121 gaaatataac gttccgacca caatggtgta aacttcttct tatacttgtg taaaccatta
     3181 aatgagtaga aatgactacc gaaatgatag acaagatacg ccattctctc tttagcaaaa
     3241 cttgtttcaa cgcgaccaac tcctgataaa ggtgccatcc ccaaatcaaa atagtggtat
     3301 cccttctctt gataataaga gaataatgat aaaaagagga aatccataac gccattcgga
     3361 attttctgtt tatcgtgacg cattaaatca atagagataa tactcttttc atagtttggc
     3421 ataatattag caaaagcaac aacttcgtgt tcagcatttt ttaccaaagc tattggggct
     3481 tgttggaaat aatctttatt aaaatatcct agtgagaaac ctttttcagg acgtccttct
     3541 aaccaagtat tagaaatctc ttccaaacta tttagtagct cttgactatg tggcgattgt
     3601 acaacttcga aatagaaacc atccttttca actctattta gggcatttct gaaaggtttg
     3661 tacttattcc ctttaagagt aaacgtttct aaattaacta aagcatcctc accaactttc
     3721 ataaagtcaa aaccatactc atgtaaaagt agtgtcaact tctgtccaat actgtaaaaa
     3781 acaaggtcat agtctagctt atcagcatca tcaataaacg attcaatagc ttcacgaata
     3841 taagtgtcat caccggctgg ttcccccata ataagacatt tattattgac aattacaaat
     3901 tggaacgcaa cgcaatcttc tccatttttt tggtaccagt agagcctttt atcatttaaa
     3961 aaggctaaac cgctatccga agaaccaccg taagcttgaa gtagtttttt ataacgatcc
     4021 acattgaaca catcaccaat ctgacaagat tttccttgta aaatcttaac caaacataaa
     4081 taaacgagag taagaatcaa cgtagccaat gctatcggct cccatgctat gatataatgc
     4141 aggcgttcaa tactaccacc tgtaatatga gccctgatag ggaatagtag tcctgcaata
     4201 taaaatagaa ctcccattaa actaacgata atgattccat ccttaatacg ctcttcccag
     4261 ctataaataa attgtttttt atagagagtt ggcttaatga ctaataagcc taacaatagt
     4321 aaaataaacc agaaagatag tcgccaacta atatgaccta aattaagata aaacaatgtc
     4381 aaagtaatcc agataatagc aattggaaaa gcatttttca ctttttgatc aatagttcta
     4441 gctaagagaa taaaacaaac ccccagcaat aaacctggaa actgccataa catttgttct
     4501 tgtaatgggt ccaagcctag cttctgcaac cacataatat aagtaatatt ttcaaaaaat
     4561 gctgttgaaa atattaagaa tgcacctaaa atacgcatca aatggctcac catggtttgt
     4621 agaacagttg atactaactc tttcgggaca ttttcataac gttgatttat ttgactacct
     4681 aaataatgga taaagaaata gatacctgca aagaatggaa taatatagta ggctaaacga
     4741 taaagtaata accatgcaac cacagtttct ttaggtagtc cctcggcagc aaaccctgta
     4801 aatagaacta attcaaaact tcctaatcca ccgggaataa gggatacaat cccgacggca
     4861 caaccaatac aaaatagtgg taaaatttta taaactggta gatgaatgcc cattaaataa
     4921 ccgataatta taaatgctgc ggccgcacat ccccattcaa aaaaagaaac aacacctaat
     4981 tttatacgag tactagatgg catatctccg aaataatggc ttcctttatg accagaaatc
     5041 caataaataa caggaaaata catactagca ccaattaata ccaaataata gtaatcaaca
     5101 ctagctttgg catgaaaaat atggctcatg attaaggcaa tcacactaat aaatgacaga
     5161 ccagaaataa gatagggtaa aaaacgagtc acttctctta ggtcactctt ctcttgacct
     5221 tttttaccat aaaaagccat gcgcaaccca atatcgatta agccaccgaa tcctgacaag
     5281 ttattaaaag tgttgataca ccaactagtc tgtaaaatat accttttgct tttatctgta
     5341 cgtaaaagtc cattcagtac aaagtcatat ccggttgtcg gtaagacagc gataaccccc
     5401 acaaggataa ttaagaaaag attcattgga gataattgcc caaaaacact ctttagttgc
     5461 tcaacagaaa tagttcgttt caaatgaatc atttcaacaa taataagcac taaaacagat
     5521 ataaaaaata caattttaat cacagaagtc agtgatttga ctttttcaat tagctttttc
     5581 aacaatttct cctttcaacg tatcgttgat aaactttttt gcaataatat taaaaacatg
     5641 ggaatcttgc ttaataatgt gatgcccaaa gccaactaaa gaataaaact ccccccttgg
     5701 aaaataagaa gcaagtttct tagaatgatt taacttaatt atgtccttat ttccaaccaa
     5761 aaccattaca ggagttgaca catgctgtaa atcagctgga ctaatcttca aatcctccaa
     5821 cataagcgaa ataacttgag ctttttgcct catatacgga aagagtttcc ctaaatagtg
     5881 aaggaattta taggcaatcc ttactaaaag aatatcccac catcgctgac catgaatagt
     5941 caggttccct gaattaagca aaagccctct aaccatacct ggaaacatcg tttgaaaaac
     6001 taaagctaaa ttggcaccat cgctatggcc taccaatata actttatcaa tctctaaatg
     6061 aactaagata tcctttaagt caactgctat ttgcctgaaa ctaatggtat ttagctttgc
     6121 atgacttttg ccatgccctc tactatccat aacaataact tggtaatact tagaaaaata
     6181 tgctatttgc ttatcaaaat agcgactact taagctattg ccatgtaaaa aaacaatcgg
     6241 ttgaccttga ccaacgacac gataataaat atctgaacca tctttagtat gtaaccagtt
     6301 ctcagtcata atcaaagtca cctctctttg aattcgtagt tatattatac tatttttaaa
     6361 gacaaattac ttaaactacc tgtattatga gaaaaaaatg attactaaaa gccgttcaac
     6421 taaatcgaac ggctaagtag tattaaaata agaatgttaa aagagctaat ataggtaaac
     6481 caccctgttt taataagatt tttttatcaa ctgtcaaagc accataaata gcaactagca
     6541 atacattgat taaaaaaaca gctacaattt cttgattctg tgaaatatat aacccataaa
     6601 ggaggaatag gccaatcaag ccgttgtata caccttgatt cttaaaaagt ttaataacgg
     6661 gtaaatatga caactcttct ttagacatac taaaaatctt cccagtcata tttgactggg
     6721 tggctaacgt ctccaaatac ataatataaa aatgttccaa agcagttaat gttgcaataa
     6781 ttattgttaa agtagacata tcttctcctc ctaaatactt tgacatacca tatcatactg
     6841 ttagtctatc actagatact ttctattaat cttcttgttt acgataaata agaagagcaa
     6901 gtatcatcga tgatactaaa aagattacta gcattctcca ttgatggtta acgtctcccg
     6961 tcaacgagat ggtctctctt aatcctgaaa cagagtaagt catcggtaaa aatggttgaa
     7021 ttgtttgaaa gaacttagga ctcaattcta ttgggtaagt tcctgcgctt gaaccaagct
     7081 ggaataataa tattaataac gacaagaagg aaccatacct attatcccat cccactaaag
     7141 cagttactaa tgccatcaaa gtccatgctg tcaataggat aataaagtag gtttttccag
     7201 ggtaatctgg tttaagacct atgaattgaa ctgcaaaaaa taagatagtt gctgctagag
     7261 ttgcaatgaa tccattgatt aataatttat ttttagccca actaaaacga ttagctggct
     7321 ctttgccaga taaagctttc gcaaaaataa cattggctga taaagctacc accatcaacg
     7381 caactgaaat catgtatggt gccattccaa caccattcgt atcaacgcta tcataatctt
     7441 catgcttgat agtgactggg ctagatactg cattggcatt atctttattt acagaaacca
     7501 tggacaactg gtccccagct ttccccaaag aacttgataa ttgagaaaca ccacctgaca
     7561 actctgtcaa cccatctgtc aactgatgtc ctcccccagc aagcttatta gaaccatctg
     7621 caattctatc agaaccacta gctaattgcc ccgctccgtc tctcaattga tccgatttac
     7681 tatctaaagt ttgagctccc tgacttgctt tttgtgttcc tgaaatcaat tgattggatt
     7741 tactatctaa agttactgca cctttgcgta attgccctac agcattcgta taatccgtga
     7801 ttccttttgt caaggtttga ctaccttgat ataactgctt agtagccgtt ggcaattttt
     7861 gaattttatc taaatctgag atagctttta atactaaatt gttagctgtt cctgacaaag
     7921 tttgaagatt ttgtgatcca ttattagaag gggctgaact agatgcttca ctaagcacag
     7981 ttttcatatt ttctatattt gaagaaatat ttgctgcaat tgtctcagct gaagtaggtg
     8041 ttccagtaac cgctgaggtg atttgagatt gttgttcagg ggttaaactt ttaaaagctg
     8101 acgttgattg taccgcagtc atttgctttt gtttatcagc agcagccgta ttcatcaaag
     8161 cctttgtact tgattcaata gttgataaaa aattaaataa cttactatta gtatcgctat
     8221 cagtagttgc tgtcgattgt aaatttgtaa taatctgatt tagattcttt aatccatctg
     8281 aaaggttctg caaagtacgt tcttgctctg gttttagttg agttgcttgt gacaattgtt
     8341 gtaaaccatc cgtcaacttt tctgatcctt ccaccaatgc ttgagactta tcatctaatt
     8401 tttgactacc ttcactcaac ttatgcacac ctgttgtgta agcagtaaca ccatctgata
     8461 atgtttctaa cccgttagat agggtcccca caccatctgt ataaaggttt attccactat
     8521 ttagtttatt agctcctcca gaaaaagttt gactagagct tgctaaaaca tctaaattac
     8581 ttgtcaaggt ttggctccca tcctgtagct tattagaacc atttaataat tcttgactcc
     8641 catcagcagc tttattgata cctgttttca tagaacccat atttttaaaa actgattcag
     8701 tataagtccc tgtaatagat tctgctacct catcttttag tttattagct gctgtctcac
     8761 tcatttttga agctacaaaa ctacgcccct tagtagtttt ataagtaata tttaaccttt
     8821 ctggtttttc tgtcatgaga gttgtcgctt ttctagaaag atctttagga aaggtaataa
     8881 ccatataata gtgacccttt tctaattctt tttctgctct tttagctgtt gtaaaatgga
     8941 aatctaagct atcattttta gacaaattgt cctccatatc tttgccaatt gaaatagttt
     9001 ttccatttaa tttagccgat ttatcttggt taacgactgc cacaggtaaa tttttagtat
     9061 tcccataagg atcccacatt gagcttagaa agataacatt atatagcgtt ggaatcagtg
     9121 caaccccagc catcgtaatc cataattttg ggctttttat gattgctttt aattcgtcta
     9181 acattttttc tcctattttg gacatattgt tcattttttt gataaaatat attatacagt
     9241 gatgatagat aatttcaaga taaaaatctc gtttttggac actgtgttta aaa
//
LOCUS       DATRTN010000070         9081 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.070, whole genome shotgun sequence.
ACCESSION   DATRTN010000070 DATRTN010000000
VERSION     DATRTN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9081
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.070"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..843
                     /locus_tag="VBE22_001550"
     CDS             <1..843
                     /locus_tag="VBE22_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEO7202995.1"
                     /translation="KNASTIVGKIQELYHTEDISVFYTEQKDDEKKQVINILRSFKES
                     DHLMIIGGDGTLSKVMTYLPQHIPCTYYPVGSGNDFARALKIPNLKETLTAIQTERLK
                     EINCFIYDKGLILNSLDLGFAAYVVWKASNSKIKNILNRYRLGKITYIVIAIKSLLHS
                     SKVQVLVEGETGQQIKLNDLYFFALANNTYFGGGITIWPKASALTAELDMVYAKGHTF
                     LKRLSILLSLVFKRHTTSKSIKHQTFKAMTVYFPKNSLIEIDGEIVELDQISLKCQKR
                     YLYM"
     gene            853..1050
                     /locus_tag="VBE22_001551"
     CDS             853..1050
                     /locus_tag="VBE22_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEO7202996.1"
                     /translation="MYQVGSLVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            1216..1455
                     /locus_tag="VBE22_001552"
     CDS             1216..1455
                     /locus_tag="VBE22_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7202997.1"
                     /translation="MSKWENGDTTPDLDNLIRLAEIFDVSLDEHVLAKTNEVKVERIY
                     ENKPLDLRKYNKIYWFIFRNIIRSLLIILAILTFL"
     gene            1615..2730
                     /gene="ychF"
                     /locus_tag="VBE22_001553"
     CDS             1615..2730
                     /gene="ychF"
                     /locus_tag="VBE22_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEO7202998.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEEEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ADPDDIDYVNQIRAFAETENAEVVVISARAEEEISELDDEDKLEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAASIIHSDFERGFIRAVTMSYDD
                     LIQYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2814..3389
                     /locus_tag="VBE22_001554"
     CDS             2814..3389
                     /locus_tag="VBE22_001554"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO7202999.1"
                     /translation="MVKMIVGLGNPGSKYNDTKHNIGFMAVDRIVKDLDVNFTEDKNF
                     KAEIGSDFINGEKIYFIKPTTFMNNSGIAVKALLTYYNISIKDMIIIYDDLDMEVGKI
                     RFRQKGSAGGHNGIKSIIAHLGTQEFDRIKVGIGRPNGRMTVINHVLGKFDKNDEIMI
                     LNTLDKVDNAVNYYLQTNDFQKTMQKYNGLK"
     gene            3386..6883
                     /gene="mfd"
                     /locus_tag="VBE22_001555"
     CDS             3386..6883
                     /gene="mfd"
                     /locus_tag="VBE22_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEO7203000.1"
                     /translation="MNIIELFSQNKVVRTWHSGLVTNSRQLVMGFSGASKAIAIASAY
                     EKLSKKIMVVTATQTDSDKLSSDISSLIGEDNVYQFFADDVPAAEFIFSSLDKSISRL
                     SALRFLKDPEKNGVLITSISGLRLLLPNPEVFSKSQYKFEIGQECYLDKLCKNLVNLG
                     YQKVSQVFSPGEFSQRGDILDIFEMTQEYPYRLEFFGDEIDGIRQFDIDTQKSLKQLE
                     SVQISPADDIILQDADFERAKKKLEGYLVTASEVQRTYLSEVLSTTENHFKHSDIRRF
                     LSIFYEKEWGILDYIPEGTPLFVDDFQKIVDRNAKLDLEIASLLTEDLQQGKSHSSLN
                     YFSDPYKQLRQYQPATFFSNFHKGLGNLKFDKLHHFTQYGMQEFFNQFPLLVDEINRY
                     KKSGATVLLQVDSQKGLNLLQENLKEYGLDLIISDKNDIVQKESQLIVGHLSNGFYFA
                     DEKIVLITEREIYHRRVKRKIRRSNISNAERLKDYNELSVGDYVVHNVHGVGKFLGIE
                     TIEIQGIHRDYLTIQYQNADRISIPVEQIELLTKYVSADGKEPKINTLNDGRFKKAKQ
                     RVAKQVEDIADDLLKLYAERSQLQGFAFSPDDNMQNDFDNDFAYVETEDQLRSIKEIK
                     QDMEGNRPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVVLVPTTVLAQQHFENFKE
                     RFSNYPVTVDVLSRFRSKKEQTDTLKRLSKGQVDIIIGTHRLLSQDVVFSDLGLIVID
                     EEQRFGVKHKEKLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLETNPGLVREAIIREIDRGGQVFYVYNKVDTIDQKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFVENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEISEKRLDAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIATKQGKSLIRQKGNAELALQIDAYLPAEYISDERQK
                     IEIYKRIRELETRADYEALQDELIDRFGEYPDQVAYLLEIGLLKAYLDLAFTELVERK
                     GNEISILFEKASLKYFLTQDYFEALSKTQLKARISETNGKMEVVFNIKHKKNYEIIEE
                     LLKFAECFIEIKSRKPVEE"
     gene            7174..7446
                     /locus_tag="VBE22_001556"
     CDS             7174..7446
                     /locus_tag="VBE22_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEO7203001.1"
                     /translation="MRLDKYLKVSRIIKRRPVAKEVADKGRVKVNGVLAKSSTDLKLN
                     DQVEIRFGNKLLTVKVLEMKDSTKKEDAIKMYEIINETRIETDEQA"
     gene            7433..7804
                     /locus_tag="VBE22_001557"
     CDS             7433..7804
                     /locus_tag="VBE22_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEO7203002.1"
                     /translation="MSKPNVVQLNNQYINDENLKKRYEAEELRRKNRLMGWVLIFVML
                     LFILPTYNLVKSYRTLQERRQEVVKLTKDYQTLTNRTENQKLLAKQLKNPDYVQKYAR
                     AKYYFSKTGEMIYPLPDLLPK"
     gene            7807..7941
                     /locus_tag="VBE22_001558"
     CDS             7807..7941
                     /locus_tag="VBE22_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEO7203003.1"
                     /translation="MNNLIETIEHFLAYSDDKLEELADKNYQLRQTIVEKEKIESREK
                     "
     gene            7941..>9081
                     /locus_tag="VBE22_001559"
     CDS             7941..>9081
                     /locus_tag="VBE22_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO7203004.1"
                     /translation="MKKVLTFLLCSLYFVSIPAISTEEPLTLSQNRRYALTQTVVDKE
                     MYFDAIPERPTTKIEISSFQDEALTITGETLVPNTLLSIVSLTINSNGIPVFTLSNGQ
                     FIKASREAIFNDLVSKQQSVSLDYWLKPSFVTYEAPYTNGVSEVKNNLKPYSRVHLVE
                     QAETEHGIYYKTDSGFWISVEDLSVADNRMAKVQEVLLEKYNKDKYGIYIKQLNTQTV
                     AGINIDRSMYSASIAKLATLYASQEQVKLGKLSLDSKFEYKDNVNQFPNSYDPSGSGK
                     LEKKADHKLYTVKELLEATAKESDNVATNMLGYYVNNQYDSMFQTQVDTISGMHWDMK
                     KRQISPQAAGKMMEAIYYQNGDIVNYLSKTDFDNTRIPKNIPVKVA"
BASE COUNT         3245 a         1254 c         1712 g         2870 t
ORIGIN      
        1 aaaaatgcct ccactattgt tggtaaaatt caggagcttt atcatactga agatatttct
       61 gtgttctata cagaacagaa agatgatgaa aaaaaacaag tcattaatat actaaggtct
      121 tttaaagaaa gtgatcatct aatgattata ggaggagatg gtaccttatc aaaagtaatg
      181 acttatctcc cccaacatat tccgtgcact tattatcctg ttggttcggg aaatgatttt
      241 gccagagctt tgaaaattcc taacctcaaa gaaaccctta ccgctattca gacagaaaga
      301 ttaaaggaaa ttaattgctt tatttatgat aaagggctga ttttgaatag tttagattta
      361 ggttttgcag cctatgtcgt ttggaaagca tctaactcta aaataaaaaa tattttgaat
      421 agatatcgtc taggaaaaat aacttacatt gttatagcta tcaaatcatt attacattct
      481 tctaaagtac aagtattagt ggaaggagag acaggacagc agataaaact aaatgacctt
      541 tattttttcg cattagcaaa taacacttat tttggaggtg gcattactat ttggcctaaa
      601 gcaagtgctt taactgcaga gttagatatg gtttacgcta aagggcatac ttttctaaaa
      661 cgattaagta ttttattatc tttggttttt aaaaggcata ctacctctaa aagtataaaa
      721 caccagactt tcaaagctat gaccgtatat tttcctaaaa atagtttgat tgaaatagat
      781 ggcgaaatag ttgagttaga tcagattagt ttaaaatgtc aaaaaaggta tttatatatg
      841 taaggagaaa agatgtatca agttggatca cttgttgaaa tgaaaaaacc tcatgcttgt
      901 gtaattaaag aaactggtaa aaaggctaat caatggaagg tactgagagt aggagctgat
      961 attaaaattc agtgtactaa ctgtcagcat gtgattatga tgagtcgtta tgattttgag
     1021 cgaaaattaa aaaaagtact gcaaccatga aagtcctttt aaacaaataa ttatagctat
     1081 atttgcaacc aatactttcc taaataatgc tagtatgccg ttggaaaatt agctattcta
     1141 atgttatcga aagaagaaag gtggtcattg acaatgacga aagttgcaga acagttattt
     1201 atttttcggt aagtcatatc aaaatgggaa aatggggata ccacaccaga tttggataat
     1261 ttaatcaggt tagctgagat ttttgacgtg agcttagatg aacatgtttt agctaaaaca
     1321 aatgaagtta aagttgaacg tatttatgaa aataaacccc ttgatctacg aaaatacaat
     1381 aaaatttatt ggtttatttt tcgaaatatt attcgatctc tactaattat tttagctata
     1441 ttaactttct tataagctct aggtattccc tttgtttcgg agtggataat ttaaatgaag
     1501 gtgaaagagt ggcataatga taagcaaatc tgaaattttc ttcttatcat accgcttttt
     1561 cttctatttt ttgatataat agtcttgatt gaaatttgaa tggagactca ctaaatggct
     1621 ttaacagcag gtattgtagg cttaccaaat gttggtaaat caactttatt taacgcaatt
     1681 acaaaagcag gagcagaagc tgctaattat ccttttgcga ctattgatcc caatgttggt
     1741 atggtagaag taccagatga gcgtctacaa aaattgacag aattgattac gcctaaaaaa
     1801 acagttccga caacctttga atttactgat attgcaggta ttgttaaagg tgcttcgaag
     1861 ggggaaggtc taggtaataa atttttagct aatattcgtg aagtggatgc tattgttcat
     1921 gtggtacgtg cgtttgatga tgaaaatgtt atgcgtgaac aaggtcgtga ggatgctttc
     1981 gttgatccta tagcagatat tgatactatt aatcttgaat taatcttagc tgatttagag
     2041 tcaatcaata aacgttatgc gcgtgttgaa aaaatggcac gaactcaaaa agataaagaa
     2101 tcagtagcag agttcaatgt tttacaaaag attaaacctg ttttggaaga tgggaaatca
     2161 gctcgtacca ttgagtttac agaagaggaa gcaaaagttg ttaaaggtct ttttttatta
     2221 acaactaaac ctgttttata tgttgctaat gttgatgagg acaaagtagc tgatcctgat
     2281 gatatcgact atgttaatca aattcgtgca tttgcagaaa cagagaatgc agaagtagtc
     2341 gtgatttctg ctcgagctga agaagaaata tctgaacttg atgatgaaga taagttagaa
     2401 tttttagaag ctattggatt aacagaatct ggtgttgata aattaacacg agctgcttat
     2461 catcttttag gccttggaac ttactttact gctggtgaaa aagaggttcg cgcttggacg
     2521 tttaagcgtg gtataaaagc tccacaagct gctagtatta tccattcaga ttttgaaaga
     2581 ggttttattc gtgcagtaac gatgtcatat gatgacctta ttcaatatgg atctgaaaaa
     2641 gcagtaaaag aagctggccg gttacgtgaa gaaggaaaag agtatattgt tcaagatggc
     2701 gatatcatgg aattccgttt caatgtctag taataaataa tcgatttgtt aacttttagg
     2761 ttaaggatat tgttaatatc cttaaccatt tttgcgtttt aatggagaag ataatggtaa
     2821 aaatgattgt tggattagga aatccaggaa gtaaatataa tgatacaaaa cataatattg
     2881 gatttatggc tgttgacaga attgtaaaag accttgacgt caactttaca gaagataaaa
     2941 attttaaagc agagataggt agtgatttta tcaacggtga aaaaatttat tttatcaaac
     3001 cgactacttt tatgaataac agtggtattg ctgtaaaagc cctgcttact tactacaaca
     3061 tttctattaa ggatatgatt atcatatatg atgatttaga catggaagtc ggtaaaatac
     3121 gttttcgtca aaaaggatct gctgggggac ataatggtat caagtctatt attgctcatt
     3181 taggtactca agaatttgat cgtattaaag taggtatagg acgtccgaat ggacgtatga
     3241 cagttattaa tcatgttcta gggaaatttg ataaaaatga tgaaattatg atattaaata
     3301 cccttgataa ggttgacaat gctgtcaatt attatttaca gacgaatgat tttcaaaaaa
     3361 caatgcagaa atacaacggt ttaaaatgaa tattatagaa ttatttagcc aaaataaagt
     3421 ggtgcggaca tggcactctg gattagtaac taatagtcgc caattagtta tggggttttc
     3481 aggagcaagt aaagctattg ccatagcatc agcctacgag aagttatcta aaaaaattat
     3541 ggtagtcact gccactcaaa ccgattctga taaattatca agtgatatat caagtttaat
     3601 tggagaagat aacgtttatc aattttttgc tgatgacgtt cctgcagcag agtttatttt
     3661 ctcttcatta gataaatcaa tttcacgact cagtgccctg aggtttctaa aagatccaga
     3721 aaaaaacggt gttttgatta ctagtatcag tggtttaagg ttactattac caaatccgga
     3781 agttttctcg aaaagtcaat ataagtttga aatcggtcaa gaatgttatt tagataaatt
     3841 gtgtaagaac ctagtcaatt taggttatca gaaagtttct caagtattta gtcctggtga
     3901 atttagccaa agaggagata ttttagatat ttttgagatg acacaagaat atccttatcg
     3961 cttggaattc ttcggagatg aaatagatgg catcaggcag tttgatattg acactcaaaa
     4021 gtctcttaaa caattagagt cagtacagat ttcgccagca gatgatatta ttttacaaga
     4081 tgcagatttc gagcgtgcaa agaaaaaact tgagggctat ctagtaacag ctagtgaggt
     4141 acaacgcacc tatttgagcg aggttttatc tacaactgag aatcatttta agcactcaga
     4201 tattcgtcgt tttctaagta tcttttatga aaaagaatgg ggaatattag actatattcc
     4261 tgagggaact ccactttttg ttgatgattt tcaaaaaatt gtagatcgaa atgctaagtt
     4321 agatttagaa atagctagcc ttctgacgga agatttacaa caaggcaagt ctcattctag
     4381 tttgaactat ttttcagatc cttataagca attaagacaa taccaaccag caaccttctt
     4441 ttcaaatttc cacaaaggat taggaaatct gaaatttgat aaacttcatc attttactca
     4501 atatggaatg caagaatttt ttaatcaatt tccattgttg gtcgatgaaa tcaatcgtta
     4561 taagaaatct ggagcgacag tactgttgca agtagattct caaaaaggtt taaatctcct
     4621 tcaagaaaat ttaaaagaat atgggttaga tttaatcatt tcagataaaa acgatatcgt
     4681 tcaaaaggag tcacaactta ttgttgggca tctttcaaat ggtttttatt ttgcagatga
     4741 gaaaattgtt ctgattactg aacgtgagat ttatcatagg cgtgttaaaa ggaaaattag
     4801 acgcagtaat attagtaatg cagaacgtct aaaagattat aatgaacttt cagttggcga
     4861 ttatgttgtt cataatgttc atggcgtagg aaaattttta ggtattgaaa caattgagat
     4921 acaaggtatt catcgtgatt atctgactat tcaatatcaa aatgctgatc gtatctctat
     4981 ccccgtagaa caaattgaac ttctaacaaa atatgtctct gcagatggca aagagcctaa
     5041 aattaatacg ttaaatgatg gtcgcttcaa aaaagcaaaa cagagagttg caaagcaagt
     5101 agaagatatt gctgatgatt tgttgaaact ttatgcagag cgtagtcaat tacaaggatt
     5161 tgcattttct cctgacgata atatgcaaaa tgactttgat aatgattttg catatgtaga
     5221 gacagaggat caattgcggt ctataaaaga aattaaacag gatatggaag gtaatcgtcc
     5281 gatggatcga ttattggttg gagacgttgg ttttggtaag acagaggttg ctatgcgtgc
     5341 ggctttcaaa gctgtcaatg accataaaca agttgttgtt ttagtcccaa caacagttct
     5401 agcccagcag cattttgaaa attttaaaga acgttttagc aattatcctg ttacagttga
     5461 tgtacttagt cgttttcgta gcaaaaaaga acaaacagat acactaaaaa gattatctaa
     5521 gggccaagtt gatattatta ttggaacaca tcgacttctc tctcaagatg ttgtattttc
     5581 ggatttaggg ttaatagtta ttgatgagga acagcgattt ggtgtaaagc ataaagaaaa
     5641 gttaaaagaa ttaaaaacaa aagttgatgt attaactttg acagcgaccc caatacctag
     5701 aacattacat atgtcaatgc ttggcattag agacttatca gtcattgaaa cacctccaac
     5761 taatcgttat cctgtgcaga cttacgtttt ggagacaaat cctggtttag ttagagaagc
     5821 tattattcgt gagatagaca gaggaggaca ggttttttat gtttacaata aagttgacac
     5881 tattgatcag aaagtttcag aacttcagga gttagtacca gaagctagta ttggttttgt
     5941 tcatggtcaa atgtctgaaa ttcaattaga aaatacatta atagacttta tcaatggaga
     6001 ttacgatgtt ttagttgcaa cgacaataat tgagacagga gtcgatattt caaatgttaa
     6061 taccctcttt gttgaaaatg cagatcatat gggcttgtca accctttatc aattaagagg
     6121 ccgtgtgggt agaagtaatc gcattgctta tgcttacctt atgtacagac ctgataaagt
     6181 tttaacagaa atttctgaga aaagactaga tgcaatcaaa gggtttacag aattgggatc
     6241 tggttttaaa atagcgatgc gtgatttatc tatacgtggt gcaggaaata ttttaggagc
     6301 ttcacaaagt ggttttattg attcggttgg attcgagatg tattcacagc ttctagagca
     6361 agcgattgcc accaaacaag ggaaatcatt aattcgtcaa aaaggtaatg ctgaattagc
     6421 cttacaaatt gacgcctatt taccagcaga atatatatca gatgagcgcc aaaaaataga
     6481 aatatataag cgtattcgtg aattagaaac tagggctgat tatgaagcct tacaagatga
     6541 gttaatagat cgttttggag aatatcctga tcaagttgca taccttttag aaataggact
     6601 tttaaaagct tatctggacc ttgcctttac tgaattagta gaaagaaaag gtaatgaaat
     6661 aagcatccta tttgaaaaag catctttgaa gtatttttta actcaagatt attttgaagc
     6721 actatcaaaa actcaactta aggctagaat tagcgaaaca aatggtaaga tggaagttgt
     6781 ttttaatatc aaacataaga aaaattatga aattattgaa gaacttctaa aatttgccga
     6841 atgttttatc gagattaagt cacgtaaacc agtagaagaa taactaagtg aaaagttgca
     6901 aggaaatttg gatactcttt tagaaaatgt ttggttcaag aagaatagta cgagcaacat
     6961 tatgtttcac gtcaaacatc tcaacttttg aaagacataa atatttttag aggttattaa
     7021 aatagaagtt ttttacttga tatttgagag aattaagaga gctattagct ctctttttgt
     7081 tacattatat tgacaaatat tatcagttca gatatttttc aaaaaaaatg ctaaaatagt
     7141 agagttaatt tagatagaag gatgtgatca tcagtgagat tagacaagta tctaaaagtg
     7201 tcgcgtatca ttaagcgtcg tcccgttgct aaagaagttg cagacaaagg acgtgtcaag
     7261 gtgaatggcg tattagcaaa gtcatcaact gacctaaagc ttaatgacca agttgaaatt
     7321 cgttttggta ataaattgtt gaccgttaaa gtattagaaa tgaaagatag cactaagaaa
     7381 gaagatgcta tcaaaatgta tgaaattatt aatgaaacaa ggatagaaac agatgagcaa
     7441 gcctaatgtt gttcagttaa ataatcaata tattaacgat gagaatctaa aaaaacgtta
     7501 cgaagctgag gagttacgcc gaaaaaatcg tttaatgggt tgggttctta tttttgtcat
     7561 gcttttattt attttaccca cttataattt agttaagagt tacagaactt tacaagaacg
     7621 tcgtcaagaa gttgtaaaat taacgaaaga ctatcagaca ttaactaata gaactgagaa
     7681 ccagaagttg ctagcaaaac aactaaaaaa tccagattac gttcaaaaat atgctcgagc
     7741 taagtattat ttctctaaga ccggcgaaat gatttaccca ttaccagacc ttttaccaaa
     7801 ataattatga ataatttaat tgaaacaata gaacattttc tagcctactc ggatgacaaa
     7861 ctagaagaat tagctgataa gaactatcaa cttaggcaaa caattgtaga aaaagaaaag
     7921 attgagagca gagaaaagta atgaagaaag ttttaacctt cttattatgt agcttatatt
     7981 ttgtatcaat cccagctata agtaccgagg agccattaac gctttctcaa aatagacgat
     8041 acgccttaac tcagacagtt gtggataagg aaatgtattt tgatgctatt ccagagagac
     8101 caactacaaa aattgaaata agcagttttc aagatgaagc tttaaccata acaggagaga
     8161 ctttagttcc aaatacttta ttgtcgattg tttctcttac aattaattca aatggaattc
     8221 cagtatttac tttgtccaat ggacagttca taaaggctag tcgtgaagct atttttaatg
     8281 atttagtttc taagcagcaa tctgtctcat tggattattg gttaaaacct tcttttgtga
     8341 cttatgaagc accttacaca aatggtgtta gtgaagttaa aaataattta aaaccttatt
     8401 ccagagtgca tttagtagaa caagcagaaa cagaacatgg tatttattat aaaacggaca
     8461 gtggtttttg gatatcagta gaagatctat cggttgctga taatcgtatg gctaaggttc
     8521 aagaagtatt attggaaaaa tataataaag ataaatatgg tatatacatc aaacaattaa
     8581 atacacagac agtagctggt attaatattg ataggtcaat gtactctgca agtattgcaa
     8641 aattagcaac actttatgct agccaagagc aagtaaaatt aggaaaactg tcattggaca
     8701 gcaaatttga atacaaagat aatgttaatc aatttcctaa tagctatgat ccaagtggta
     8761 gtgggaaatt agagaaaaaa gctgatcata aattatatac cgttaaggag ttacttgaag
     8821 caactgccaa ggaatcagat aatgttgcaa caaatatgct aggttattat gtcaataatc
     8881 aatatgattc aatgtttcaa acacaagtag acacaatttc tggtatgcac tgggatatga
     8941 aaaaacgcca gatttctcca caagctgcgg gtaaaatgat ggaagcaatt tattatcaaa
     9001 atggagatat tgttaattac ctatcaaaga ctgattttga taatacaaga atccctaaaa
     9061 atataccagt taaagtagca c
//
LOCUS       DATRTN010000071         8505 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.071, whole genome shotgun sequence.
ACCESSION   DATRTN010000071 DATRTN010000000
VERSION     DATRTN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.071"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..91
                     /locus_tag="VBE22_001560"
     CDS             <1..91
                     /locus_tag="VBE22_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEO7203005.1"
                     /translation="FSTTYRNRRKRFGLRMNLIAGMINRELGF"
     gene            393..1199
                     /locus_tag="VBE22_001561"
     CDS             393..1199
                     /locus_tag="VBE22_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7203006.1"
                     /translation="MSKKIILGILSLLSVVTLVACGSSDKQLQDKVEKKGKLVLAVSP
                     DYAPFEFKALVNGKDKVVGADIELAQAIADELHVKLEVSPMSFDNVLSSLQTGKADMA
                     ISGLSYTKERAKVYDFSKPYYTTENAVLVRKSDLGKYTSIESLKGKKIAVQKGSIEEG
                     VSKKSFKNSHVVSLTAMGEAISELKSGKVEAVDLEKPVAEGYLAQNPDLVLAKFALKT
                     GEGDAKAVALPKDSGKMLKTVNKVIKRLEKEDKYKVYISDAAKLTGNQVE"
     gene            1282..1785
                     /locus_tag="VBE22_001562"
     CDS             1282..1785
                     /locus_tag="VBE22_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEO7203007.1"
                     /translation="MVQGSINIEKYKDLALQKQGEHRKFLASLKKKAPKNLDKIVQQI
                     HQEVFQEIDCTACANCCKTLGPLFTEADISRISKHFRMKLSAFEDLFLEVDEDGDKVF
                     KAMPCPFLGDDNLCSIYDVRPKACREFPHTDRKKIYQINNLTIKNTLTCPAAYLFVEK
                     LKSRIGK"
     gene            1889..2065
                     /locus_tag="VBE22_001563"
     CDS             1889..2065
                     /locus_tag="VBE22_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEO7203008.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            complement(2162..2539)
                     /gene="mscL"
                     /locus_tag="VBE22_001564"
     CDS             complement(2162..2539)
                     /gene="mscL"
                     /locus_tag="VBE22_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEO7203009.1"
                     /translation="MIKELKEFLFKGNVLDLAVAVILGAAFNAIITSLVKDVITPLIL
                     NPVLKAAGVSNIAQLSWNGVAYGNFLSAVINFLIVGTTLFFIVKAANKVMAKKPAEEE
                     IIEVVEPTQEQLLAEIRDLLANK"
     gene            2663..4537
                     /gene="dnaG"
                     /locus_tag="VBE22_001565"
     CDS             2663..4537
                     /gene="dnaG"
                     /locus_tag="VBE22_001565"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO7203010.1"
                     /translation="MKKLLKKFTQKRKNDIIKCKTIMGYFCGGHDLAIDKEKISEIKN
                     SVNIVDVIGEVVGLTKTGRNHLGLCPFHKEKTPSFNVIEDRQFFHCFGCGRSGDVFKF
                     VEDYQHISFLDSVQVLAERSGIPLDTNFKGQVPKKPKANQSLLDIHRVASGFYHAYLM
                     TTNDGERARQYLAERGVTEDLIKHFQIGLSPGGQDFLYRRLAKEFDEKTLMSSGLFNY
                     SENSNQFYDSFNNRIMFPLTNDIGEVIAFSGRVWTQEDIDRKQAKYKNSRATPIFNKS
                     YELYHLDKARAVINKAHEVYLMEGFMDVIAAYRAGIENVVASMGTALTNEHVRHLKRF
                     TKKVVLTYDGDRAGQNAIDKSLELLSDMTVDIVRIPNKMDPDEFLQANSAEDFKQLLE
                     NGRISNTEFYIHYLKPENTDNLQSEIAYVEKIAKLIAKSPSITAQNSYITKVAELLPD
                     FDYFQVEQSVNNERLHHRSQQQASSSVQTSATVQLPQTGKLSAITKTEMQLFHRLLNH
                     PYLLNEFRNRDNFYFDTTEIQVLYELLKESGEITSYDLSQESDKVNRTYYIILEEQLP
                     VEVSIGEIEAVEKARDRLLKERDLRKQSQLIRQSSNQGDEEGALAALENLIAQKRNME
                     "
     gene            4545..5654
                     /gene="rpoD"
                     /locus_tag="VBE22_001566"
     CDS             4545..5654
                     /gene="rpoD"
                     /locus_tag="VBE22_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019787874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEO7203011.1"
                     /translation="MAEKKGNTTFNVQVADFIRNHKKQGTAIDDEVTEKLVIPFVLDA
                     DQIDDLLERLTDGGISITDKEGNPSTKYVVEGPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELAVAVAEGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLKDFMED"
     gene            5763..6104
                     /locus_tag="VBE22_001567"
     CDS             5763..6104
                     /locus_tag="VBE22_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEO7203012.1"
                     /translation="MSEVKQYSEEEVGKIKDRILEALEMVIDPELGIDIVNLGLIYEI
                     RFEDNGRTEIDMTLTTMGCPLADLLTDQIHDVMKTVPEVTETEVKLVWYPAWSVDKMS
                     RYARIALGIRS"
     gene            6194..7048
                     /gene="rfbD"
                     /locus_tag="VBE22_001568"
     CDS             6194..7048
                     /gene="rfbD"
                     /locus_tag="VBE22_001568"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEO7203013.1"
                     /translation="MILITGANGQLGSELRHLLDERTQEYVAVDVAEMDITNAEMVDK
                     VFEEVKPSLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKAAAKHDATLVYISTD
                     YVFDGEKPVGQEWEVDDLPDPKTEYGRTKRMGEELVEKYTSKFYTIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQHGRPTWTRTLAEFMTYLAENQKDFGYYHLSNDAKEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLEKAKATGFVIPTWQDALKE
                     FYKQEVKK"
     gene            7165..8319
                     /locus_tag="VBE22_001569"
     CDS             7165..8319
                     /locus_tag="VBE22_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEO7203014.1"
                     /translation="MQDVFIIGSRGLPARYGGFETFVSELINHQKSFDIKYHVACLSD
                     KEHHTHFNFADADCFTINPPQLGPARVIAYDIMAINYALDLVKTHDLKEPIFYILGNT
                     IGAFIWHFANKIHKVGGLLYVNPDGLEWKRSKWSRPTQRYLKYAEKCMTKNADLIISD
                     NIGIENYIQSTYSNVKTRFIAYGTEINSRKLSSDDPRVKQLFKKWNIKSKGYYLIVGR
                     FVPENNYETAIREFMASDTKRDLVIICNHQNNPYFEKLSLKTNLQQDKRVKFVGTLYE
                     KDLLDYVRQQAFAYIHGHEVGGTNPGLLEALANTDLNLVLDVDFNKSVAGLSSFYWAK
                     KEGDLAKLINDSDQQQDLSTYGDRAKAIIQENYTWKKIVEEYEDLFLNES"
     gene            8309..>8505
                     /locus_tag="VBE22_001570"
     CDS             8309..>8505
                     /locus_tag="VBE22_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7203015.1"
                     /translation="MKVNILMATYNGEKFLAQQIESIQKQTFKEWNLLIRDDGSSDKT
                     CDIIRNFTAKDSRIRFINENE"
BASE COUNT         2940 a         1262 c         1697 g         2606 t
ORIGIN      
        1 attttcaaca acctatcgaa atcgacgcaa acggtttgga ttacgaatga atttgattgc
       61 tggaatgatc aaccgtgaac taggatttta gtttcgcagg aagtctattt tagcaaggag
      121 agtgactaaa tcgaattatt atcattttct tactagacag cgactgtaca aggttctgaa
      181 agttagttga ttattaatga tagttagcat tttttaagaa ttggaatact tttaaaaggt
      241 gattgtttta attagttata gttgttatta aaaatcgtct aacagcaagc atagttatcg
      301 gtgtaattta ctgaattttt attcaaaaaa acttgactta tgtataaaga tgatttatac
      361 ttgttcctat cataataaaa ggaagatagt ttatgtcaaa aaaaataata ttaggaattt
      421 tatctctttt atctgtcgtt actttggtgg cgtgtggttc atcagacaaa cagctacaag
      481 ataaagttga gaaaaaaggg aagttagttt tagcggtgag tccagattat gctccctttg
      541 agtttaaagc gcttgttaat gggaaagata aagttgtcgg cgcagatatt gagttagctc
      601 aagctattgc tgatgagctt catgtcaaac tagaggtatc tcctatgagt tttgacaatg
      661 ttttatcaag tttacaaaca ggtaaggctg atatggctat ttctggatta tcatatacta
      721 aagagcgtgc taaagtctat gatttttcaa aaccttatta cactacagaa aatgcggttc
      781 ttgttaggaa gtctgattta ggaaaatata cttcaatcga aagccttaaa ggtaaaaaaa
      841 tagcagtaca aaaaggaagt atcgaagagg gagtttctaa gaaatcattc aaaaattctc
      901 acgtcgtgtc actgacagcg atgggagaag cgattagtga gttaaaatca ggcaaggtag
      961 aagctgttga tttagaaaaa cccgtagctg aaggttactt ggcccagaat ccagaccttg
     1021 ttcttgctaa gtttgccctt aaaacaggag aaggggatgc taaggctgta gctttaccta
     1081 aagacagtgg aaaaatgttg aagactgtta acaaagtgat aaagagactt gaaaaagaag
     1141 ataaatataa agtttatatt tcagatgcgg ctaaattaac tggcaatcaa gttgaataat
     1201 attaaaaagc ctagtcatag gcttttttgg atgttataat atggcttgac taagaataat
     1261 agcataaagg cagaggaata tatggttcag ggttcaatta atattgaaaa atataaagat
     1321 ttggcgctcc aaaaacaagg cgagcatcgt aaatttttag caagtttaaa aaagaaagct
     1381 cctaaaaact tagataaaat agtccaacag atacatcaag aggtttttca agaaattgat
     1441 tgtacagcat gtgctaattg ttgtaaaact ttgggtcctc tatttacaga agctgatatt
     1501 tctaggattt ctaaacattt ccggatgaag ttatcggctt ttgaagattt atttttagaa
     1561 gtagatgaag atggagataa agtatttaaa gcgatgcctt gtccgttttt aggcgatgat
     1621 aacttatgta gtatatacga tgtcagacct aaagcttgtc gtgaatttcc acatactgat
     1681 cgtaagaaaa tctatcaaat taataactta acgataaaaa atactcttac ttgtccagcg
     1741 gcataccttt ttgtggaaaa attgaaatca cgtataggga aataataacc cagagaaagt
     1801 atcataaaaa tattgacaat ctgcttttat ttgggtatcc tagtaagtga acgtgaaaat
     1861 gttacaatat cttgaggagg tgaaacatat gtcaaaaaca gtagtacgta aaaacgaatc
     1921 acttgacgat gcacttcgtc gtttcaaacg ttctgtgact aaagctggta ctcttcaaga
     1981 atcacgtaaa cgtgaattct acgaaaaacc ttctgtaaaa cgtaaacgta aatcagaagc
     2041 agctcgcaaa cgtaaaaaat tctaattaga tagaatatga aaaaacagtt cagaattgag
     2101 ctgttttttc atatattata actataagtt aataaaaaaa ctcggtaatc cgagtctttt
     2161 attatttgtt agcgagtaag tcgcggattt cagcaagtaa ttgctcttgt gttggttcaa
     2221 caacttcaat aatttcttct tcagcaggtt tcttagccat aaccttgtta gcagctttaa
     2281 cgatgaagaa taaagtagta ccaacgatta agaaattaat aactgcactt aagaaattac
     2341 cgtaagcaac accattccat gaaagctgtg cgatatttga aacgccagca gcttttaata
     2401 caggattaag gataagcggt gtaataacgt ctttaacaag tgaagtgatg atagcattaa
     2461 aagctgcacc taaaataaca gcaacagcaa ggtctaaaac attaccctta aataaaaatt
     2521 cttttagttc tttaatcatg tgaacaagct ccttaatatt atttaattca cgttcattat
     2581 agcagatttc ttttgagaaa tccaagtaat aggtttcaaa ttatctgtaa atttttatat
     2641 taaatcagtg aagaagaaat ccttgaaaaa gcttttgaaa aaatttacac agaaaagaaa
     2701 aaatgatata atcaaatgta aaactataat gggttatttc tgtggaggtc atgacttggc
     2761 aattgataaa gaaaaaattt ctgaaatcaa gaacagtgtt aacattgttg atgtcatcgg
     2821 tgaagttgtc ggtttgacaa aaacaggacg caatcatctt ggtttatgcc catttcataa
     2881 agaaaaaacg ccatctttta atgttatcga agataggcag ttttttcatt gttttggttg
     2941 tggtcgctct ggtgatgtct ttaaatttgt ggaagattac caacatatta gttttttaga
     3001 tagtgtacaa gttctagctg aacgttcagg aatacctcta gatactaatt ttaaggggca
     3061 agttcctaaa aaacctaaag ctaatcagtc gcttttagat attcatagag ttgcttcggg
     3121 attttaccat gcctatttaa tgacaactaa tgatggtgaa cgcgctagac aataccttgc
     3181 cgagcgcgga gtgacagagg acttaattaa acattttcaa ataggattat cgccgggagg
     3241 gcaggatttt ctatatcgtc gtctggcaaa agagtttgat gaaaaaactt tgatgtcttc
     3301 tggtttgttc aattattcag aaaatagcaa tcagttttac gattcattta ataatagaat
     3361 tatgtttccg ttaactaatg atattggtga agtcattgcg ttttcaggac gagtttggac
     3421 tcaagaggac attgatagaa aacaagctaa atataaaaat tctcgtgcca cccctatttt
     3481 taataagtct tacgagcttt accatttaga taaagcgcgt gctgtaatta ataaagcaca
     3541 cgaagtttac ctaatggaag gctttatgga tgttattgca gcttatcgtg caggaattga
     3601 gaatgttgtt gcttctatgg ggaccgcttt aaccaatgaa cacgttagac accttaaaag
     3661 atttactaag aaagttgttc tgacttatga tggtgataga gctggtcaaa acgcaattga
     3721 caagtcttta gaactgctat cagatatgac tgttgatatc gttaggatac ctaataaaat
     3781 ggaccctgat gaatttctac aagcaaattc agcagaagat tttaaacaac tcttagaaaa
     3841 tgggcgcatc agtaatacag agttttatat ccactactta aagcctgaaa acaccgataa
     3901 tttacaatct gaaattgctt atgttgaaaa aatagcgaaa ttaattgcta aatctccatc
     3961 gataacagca caaaattcat atattacaaa agtggctgaa ctcttgccag attttgatta
     4021 ttttcaagta gaacaatcgg ttaataacga acgattgcat catagaagtc aacagcaagc
     4081 ttcatcttca gtacaaacaa gtgctacagt gcaattacca cagacaggta agttatcagc
     4141 tatcactaaa acagaaatgc aattgtttca tcgattattg aatcatccct acctgttaaa
     4201 tgaattccgt aatagggata atttttattt tgatacaaca gaaatacaag ttttatatga
     4261 gttgctaaaa gaaagtggtg agatcacgtc ttatgattta tcacaagaaa gtgacaaagt
     4321 taatcgtact tattatataa ttttagagga acaattacct gtagaagtat ctattggaga
     4381 gattgaagcc gtcgaaaagg cacgtgaccg tttattgaaa gaacgtgatt taagaaaaca
     4441 aagccagtta attagacaat ctagtaatca aggtgatgaa gaaggagcct tggctgcatt
     4501 agaaaattta attgctcaaa aaagaaatat ggaatgagga acctatggca gagaaaaaag
     4561 gaaatacaac ttttaatgtt caagttgcgg attttattcg caatcataaa aaacaaggta
     4621 ctgcaattga tgatgaggta acagaaaaac ttgttattcc ttttgtttta gatgcagatc
     4681 aaattgatga tttgttagaa cgattgacag acggtggcat ttcaatcaca gataaagaag
     4741 gcaatccttc aacaaagtat gtggtagaag ggccaaaacc tgaagaattg actgatgaag
     4801 agctcatagg aagtaattct gctaaagtca atgacccagt acgtatgtac ttgaaggaaa
     4861 ttggtgttgt tcctctttta acgaatgaag aagaaaaaga attggctgtt gcggttgctg
     4921 agggagattt aatggctaaa caacgtttag ctgaagcaaa ccttcgtctt gttgtctcaa
     4981 ttgcaaaacg ttatgttgga cgtggaatgc aatttcttga tttaattcaa gagggtaaca
     5041 tggggctcat gaaagctgtt gataaatttg attattcaaa gggattcaaa ttttcaactt
     5101 atgcaacatg gtggattcgt caagctataa cacgtgcaat tgcagatcaa gcacgtacga
     5161 ttcgtattcc tgttcatatg gttgaaacga ttaataaatt ggtccgtgaa caacgtaatc
     5221 ttcttcaaga acttggtcaa gatccaactc ctgagcaaat cgcagaacgc atggatatga
     5281 caccagataa agtacgtgaa atcctaaaaa ttgctcaaga gccggtatcg ctagaaacgc
     5341 caatcggtga ggaagatgat agccatttgg gtgatttcat tgaagacgaa gttattgaaa
     5401 atccagtaga ttatactaca cgtgttgttc ttcgtgagca attagatgag gttttagata
     5461 ctcttactga ccgtgaagag aatgttcttc gtttacgctt tggtcttgac gatggtaaaa
     5521 tgcgtacgtt agaggacgta ggtaaagtat ttaatgttac tcgtgagcgc attcgtcaga
     5581 ttgaagctaa agcccttaga aaacttcgcc atcctagtcg tagcaagcaa cttaaagatt
     5641 tcatggaaga ttgattgctt ctcgataatc gtttttagaa taggaagttt agtttctaat
     5701 aaaaatgatt tgcaaaggag ttatcttaaa gacggtatta atattttcag agaggtatta
     5761 taatgtcaga agtaaagcaa tatagcgaag aagaagttgg aaaaataaaa gatcgtatcc
     5821 tagaagcttt ggagatggtt atcgatcctg aattgggaat agatattgtt aaccttgggt
     5881 taatttatga gattcgcttt gaagataacg gtcgaactga aattgatatg actttaacga
     5941 ctatgggatg cccgttagca gatttattga cagatcaaat tcatgatgtt atgaaaacag
     6001 tcccagaggt aactgaaacc gaggtgaaat tggtatggta cccagcctgg tctgttgata
     6061 agatgagtcg ttatgctcgt atcgctttgg gaattcgttc ataagataaa gagtaaaggg
     6121 atgaaatatt ccctttttta gtgtacaata aacgaagaat taaaagttaa acttattttt
     6181 aaaggagcaa ggaatgattt taattacagg ggcaaacggg caattaggta gtgaattacg
     6241 tcacctttta gacgaacgaa ctcaagagta tgttgctgtt gatgtagctg aaatggatat
     6301 tacaaatgcc gagatggtgg acaaagtctt tgaagaggtg aagccatctt tagtttacca
     6361 ttgtgcagct tacacagctg ttgatgcggc agaagatgaa ggaaaagagc tagactttgc
     6421 aatcaatgtc actggtacag agaatgtcgc taaggctgct gctaaacatg atgcaacact
     6481 tgtttacatt tcgacggact acgtgtttga tggtgaaaaa ccagtggggc aagaatggga
     6541 agtagatgat ctgcctgatc ccaaaacaga gtacggtcgt acaaaacgta tgggtgaaga
     6601 gttagttgaa aaatatactt caaaattcta taccattcgc acagcgtggg tatttggtaa
     6661 ctatggtaaa aactttgtct ttacgatgca aaaccttgca aaaacacaca aaactctaac
     6721 tgttgttaac gaccaacatg gacgaccaac atggacacgt actttggctg agtttatgac
     6781 ttatttagct gaaaatcaaa aagattttgg ttactatcat ttatcaaatg atgctaaaga
     6841 agatacaaca tggtatgatt ttgcggttga aatcttaaaa gacacagatg tggaggttaa
     6901 gcctgttgat tctagtcaat tcccagcaaa agcaaaacgt cctttaaatt caactatgag
     6961 tttggaaaaa gcgaaagcaa caggatttgt gattcctact tggcaagatg ctttaaaaga
     7021 gttctacaag caggaagtta aaaaataaga tcaaaacaaa gagtagtaag gctagagaat
     7081 ccttttagct tactgctctt tttagctaac ttttctaatt tatggtataa ttgtatggat
     7141 tgtttagcta gaatggagaa gatgatgcaa gatgttttca ttataggaag tagagggttg
     7201 ccagctcgtt acggtggttt tgaaactttt gtttcagaat tgattaatca tcaaaaaagt
     7261 ttcgacataa aataccatgt tgcatgcctt agtgataaag aacatcatac tcattttaac
     7321 tttgctgacg ctgattgttt tactataaat cctccccaat tagggccagc acgtgtgatt
     7381 gcttatgata ttatggccat taattatgcc cttgacttgg ttaagacaca tgatttaaaa
     7441 gagcctattt tttatatttt aggaaataca attggtgcct ttatttggca ttttgccaat
     7501 aaaatacata aagtcggtgg cttattgtat gttaatccgg atggtttaga gtggaagcga
     7561 tcaaagtggt ctcgtcccac acagcgttat ttaaaatacg ccgaaaaatg tatgactaaa
     7621 aatgcagacc taattatttc tgataatatt ggtattgaaa attacattca atctacctac
     7681 tctaatgtga agacaaggtt cattgcttac ggtacagaga ttaattctag gaaattatcg
     7741 tcagatgatc cacgtgtcaa acagttgttt aaaaaatgga atattaagtc taagggttac
     7801 tatctaatcg ttggtcgatt tgtccctgaa aacaattatg aaacggctat tagggagttc
     7861 atggcttcag atactaagcg tgatttagtt attatctgta accatcaaaa taacccctac
     7921 tttgaaaagt tgtccttaaa gacaaacctt caacaagata aaagagttaa gtttgtaggt
     7981 acgctctatg aaaaagatct gctggattat gttcgtcaac aagcctttgc ttatattcat
     8041 gggcatgaag ttggcggtac taatccagga ctgcttgagg ctttagctaa tactgatttg
     8101 aatcttgttc tagatgttga tttcaacaaa tcagtagcag gtctctcaag tttttactgg
     8161 gctaaaaaag agggggattt agctaagctt attaacgata gtgatcaaca acaggattta
     8221 tctacttacg gtgatcgagc aaaagccatt attcaagaaa actatacttg gaaaaaaatt
     8281 gttgaagaat atgaggattt gtttttaaat gaaagttaat attttaatgg ccacctacaa
     8341 tggagagaag tttctggcgc agcaaatcga aagtatccaa aagcaaacct ttaaagagtg
     8401 gaacctccta atcagagatg atggttcaag tgacaagaca tgtgatatta ttagaaactt
     8461 tactgctaaa gattctcgaa ttcgtttcat taatgaaaat gagca
//
LOCUS       DATRTN010000072         8240 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.072, whole genome shotgun sequence.
ACCESSION   DATRTN010000072 DATRTN010000000
VERSION     DATRTN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8240
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.072"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            135..1418
                     /locus_tag="VBE22_001571"
                     /pseudo
     CDS             135..1418
                     /locus_tag="VBE22_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644904.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
     gene            1573..1812
                     /locus_tag="VBE22_001572"
     CDS             1573..1812
                     /locus_tag="VBE22_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7203016.1"
                     /translation="MTFFLNSLLKELIIVNRLREYRIKASISQLALAKSVGVARQTIN
                     LIENNKYNPSLDLCIRLAECLQTDLNTLFWDSRHL"
     gene            1912..2445
                     /locus_tag="VBE22_001573"
     CDS             1912..2445
                     /locus_tag="VBE22_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEO7203017.1"
                     /translation="MKEKKNLGIKLIKSLYGINGPFDEYREQKINYLGNLLFATTSLY
                     HILSSIIAWFTVSKYPTETAYTLLALTFFLYFCIFLIVIPSYLNSHDLITPLEVSKND
                     YPVAIKKNKQKFIKIGIFYTVIQWLILGLTTNNNTPLSVSLFSLKVILSSIMSGVAYN
                     GVMYLIFKNNVDIEKDE"
     gene            complement(2521..3015)
                     /gene="tpx"
                     /locus_tag="VBE22_001574"
     CDS             complement(2521..3015)
                     /gene="tpx"
                     /locus_tag="VBE22_001574"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEO7203018.1"
                     /translation="MTTFLGNPVTFTGKQLQVGDIAKDFLLIATDLSQKSLKDFEGKK
                     KVISVVPSIDTGICSKQTRTFNEELSELDNTVVITVSMDLPFAQKRWCSAEGLDNVIL
                     LSDFYDHSFGQEYALLMNEWHLLTRAVLILDEHNKVTYTEYVDNVNSDVDYEAAINAA
                     KILP"
     gene            complement(3088..3747)
                     /locus_tag="VBE22_001575"
     CDS             complement(3088..3747)
                     /locus_tag="VBE22_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEO7203019.1"
                     /translation="MDLFLSLERKFKAASDKEVSKQQEAYLRHHFKCYGIKSPERRML
                     YKELIKAAKRQAKIDWQLLDKCWQSDYREYHHFVLDYLLAMSQFITYNDCSRLEFYAR
                     HQQWWDSIDVLTKVFGNLSLKDDKVMNLLSEWSLDQDFWMRRLAIEHQLGFKEKTNTD
                     ILSLFILRNTGSQEFFINKAIGWALRDYSKYNKVWVKDFISNHYDELSTLSIREGSKY
                     L"
     gene            complement(3747..4509)
                     /locus_tag="VBE22_001576"
                     /pseudo
     CDS             complement(3747..4509)
                     /locus_tag="VBE22_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348145.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
     gene            complement(4514..5155)
                     /locus_tag="VBE22_001577"
     CDS             complement(4514..5155)
                     /locus_tag="VBE22_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7203020.1"
                     /translation="MVSEQSEIVTSKYQKIAVAVAQRIANGDYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLQILTLKHGSGAIILSKEKAIEFLNQYETSHSVAILKGKIR
                     DNIKAQQQEMEELATLVDDFLLQTRAVSKQYPLAPYEIIVSEDSEHLGKSIGELNVWH
                     QTGATIVAIEHEGKFIVSPGPFSVIEQGDHIFFVGDEDVYARMKTYFNLRMGL"
     gene            complement(5319..5870)
                     /locus_tag="VBE22_001578"
     CDS             complement(5319..5870)
                     /locus_tag="VBE22_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7203021.1"
                     /translation="MENQKQDIKTSVTYEEKVIAKIVGHALESVDGLLAVSGGFFSNL
                     KNSVVNSDSVTDGVNVEVGTKEVAVDLDIVVEYGKDIPAIVESIKAIVSQNVEVMTHL
                     KVVELNANVVDVKTKAEHEADSVTVQDRVSDAAQATGNFASEQAGKAKAAISSGAEKT
                     KEAVSNGTEAAKEKISEARTSES"
     gene            complement(5892..6089)
                     /locus_tag="VBE22_001579"
     CDS             complement(5892..6089)
                     /locus_tag="VBE22_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO7203022.1"
                     /translation="MSEEKFDAKVDKVSGSVKESVGKLTGDKEVESEGKVDKLKGHAK
                     EKLADIKDTIKGASESFKKKD"
     gene            complement(6136..6678)
                     /locus_tag="VBE22_001580"
     CDS             complement(6136..6678)
                     /locus_tag="VBE22_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO7203023.1"
                     /translation="MTETYIKNTTNNSGTTAVRGELTFEDKVIEKIVGIAIEHVDGLL
                     AVNGGFFSNLKNSVVNSDSVTDGVNVEVGKKQVAVDLDIVAEYQKHVPTIFADIKKVV
                     EAEVKRMTDLEVVEVNVNVVDIKTRAQHEEDSVTLQDRVTSAAQATGEFASNQVSNVK
                     SAVGNGVDKVEDMKSEPRVQ"
     gene            complement(6734..6928)
                     /locus_tag="VBE22_001581"
     CDS             complement(6734..6928)
                     /locus_tag="VBE22_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEO7203024.1"
                     /translation="MSEFVRKYRYPLGGAVIGLVLAAMIVTIGFFKTILALVIIVLGA
                     YAGLYVQRTGMLDQFFNKRK"
     gene            complement(6938..7519)
                     /gene="amaP"
                     /locus_tag="VBE22_001582"
     CDS             complement(6938..7519)
                     /gene="amaP"
                     /locus_tag="VBE22_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEO7203025.1"
                     /translation="MSKGLKSLYTLLGLISLTLLGFVAVISKQHIYLPSFNWLDWDFN
                     LPSPIDVGMYHYFFWGALVLFVIVLLAILVVLFYPRRYTEYKLADKTGKLMLKKSAIE
                     GFVKTEVLKTGLIKSPSVTAHLYKKKVKVDVKGLLTSRTNVPEQLEHIQSDVELGLKE
                     FFGLEKKMNTRVFVKQVEEENVGNAKTNKSRVE"
     gene            complement(7534..7782)
                     /locus_tag="VBE22_001583"
     CDS             complement(7534..7782)
                     /locus_tag="VBE22_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO7203026.1"
                     /translation="MSLIWSLIVGAIIGAIAGAVTNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLAGMALIPSIVGAIIVVIVTSFVLGKMNN"
     gene            complement(7830..8075)
                     /locus_tag="VBE22_001584"
     CDS             complement(7830..8075)
                     /locus_tag="VBE22_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO7203027.1"
                     /translation="MGLIWSLIVGAIIGAIAGAITNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLADMALIPSIVGAIIVIIVTSFVLGKKS"
BASE COUNT         2773 a         1626 c         1158 g         2683 t
ORIGIN      
        1 attaattcct atcattcacc gctttatttt aggataacta aacaatttat tgtttaaaac
       61 gaaaggattt atgaatgtct aggaaaacat ttaaacgtat actatcgatt ggagtttgca
      121 cgctcgtact atcgatgagt ctttattaca ctgaaaaagc ccacgctatt actggtccta
      181 gtgaccgcca atacgtagaa aacccaaatc ctcacattat tgtaaatgtt acaggtactg
      241 atcaaaacgg aaatagcatt ttaccgcatt atatcgaagt caatgtaaag atgggacaaa
      301 ctttaagtaa agaagaaatt ctagattata ttgctcgaaa tttaaactct agtgttggag
      361 gagaaagtaa aaacgttcaa tacagcaaca tcgagtttaa ggaaagtgct tatctgaagc
      421 gtcaattaga tgatggcaag acagaagaaa tagcaattga taacgacggt gttactgtac
      481 ctaaagacgg tccaaacaaa ttttggattg acgttccagt aacttgtacg gtaaattcaa
      541 taactccaaa gctccataaa gtagaatggg gtacacctat ttcagtttta cattatgtac
      601 agttcgttga taaaactaca ggtaaaactt tagaggattt taaaactatt gattttgctg
      661 aaatcgacct tggaattcga cataccggtg atagcattac cgataaggaa ctatataact
      721 cagcttacag tgccttttta aggagtaaat taactaagca aggctatcaa ttccaataca
      781 gaattagtac agatgttcaa caaaatcttc aagtcaccaa aaaactttac aactacgatg
      841 taaatgaaga attgattaac tatcaaatcg aaaacaatag acccacacta aacctatcta
      901 ataaagccga gactgatatc tttttcgaaa gatattatgt ttctagagat ggagatagtt
      961 tagcacgtac agaatcaacc atttcgatta aaatggttga tgccaaaact gaaaaagcgc
     1021 tatttaacca cacattaacg ggttatagac attggcaact gtttccaatg tctataacag
     1081 actctttgaa gaaaatctta tcccaactac aaaatcagga gaaagatatt ttattcaaaa
     1141 tatgaaaaaa aacagctgaa caagaatata ctgtttacct ttcagaaaca ccttattcta
     1201 aagagaacgc tcccgtaatt tcttatgatg caagacctgt tgattgggat tatcactcag
     1261 gcgcttcagg atcacttgaa aatcagccta acatctatac tgaagaagat tcaactgaat
     1321 ttttgggtaa taaaccacaa gcagcttgtt atccaaacaa acaatttgct tgcgaaaata
     1381 ctgactctaa atacaactat agctatttag aaaaatagtt ataaccgata gggttaatct
     1441 ctcattatct tatactgatt aaaatcatta aacttgtttc cattttgact ggaaacaagt
     1501 tttttatatt tatttgtcac atataattga cactcttcga aaaatgatat atacttaaaa
     1561 tgtcatctat atatgacatt cttcttaaat tctttactaa aggagctaat aatcgtgaat
     1621 cgtcttagag aatacagaat taaagctagc atatctcaat tagctttagc aaaatcagtt
     1681 ggtgtcgcca gacaaacaat aaaccttatt gaaaataata aatacaatcc ttcgctagat
     1741 ctttgtataa gactagcgga atgcctacaa acagacctca atactctttt ttgggatagt
     1801 agacatctat aacagattag actctctaaa tctatgaaaa ctcgggaggt taggtcgtgt
     1861 tattatttca atctcaaact ttattaaata gttcaaggat aggagaatat tatgaaagaa
     1921 aagaaaaatt tggggataaa attaataaaa tccctatatg gtattaatgg cccattcgat
     1981 gaatacagag aacaaaaaat caattacctt ggaaatctat tatttgcaac cactagtctc
     2041 tatcacattt tatcctctat catcgcctgg ttcacggtaa gtaaatatcc cacagagact
     2101 gcatatactc tcctagcttt aacattcttt ctatattttt gtatttttct aattgttatt
     2161 ccctcttatc tcaacagtca tgatttaata acgccactag aagtctcaaa aaatgactat
     2221 ccagtagcaa taaagaaaaa caaacaaaaa tttatcaaaa tcgggatatt ttatactgtc
     2281 atccagtggc ttatcttggg tttaacaact aacaataaca ctccgctgag tgtatcacta
     2341 ttttctttaa aagttatttt gtctagtatt atgtccggtg tagcttacaa tggagttatg
     2401 tatctaatat ttaaaaacaa tgttgatatt gaaaaagatg aataatgaca acatttcatc
     2461 tctaattgtt ctcactgtct atataaataa taaggctgga agattttcca gccttattat
     2521 ttatggtaat atctttgcag cattaatcgc cgcttcgtag tcaacatctg aattaacatt
     2581 gtcaacgtat tcggtgtaag tcaccttatt atgctcatcc aaaataagaa ctgcacgcgt
     2641 taacaaatgc cactcattca tcaaaagagc gtactcttga ccaaacgaat gatcataaaa
     2701 atctgataat aaaatcacgt tatcaaggcc ttcagcgctg caccaacgct tctgagcaaa
     2761 tggaaggtcc atagaaacag taataaccac tgtgttatct agctctgata attcctcatt
     2821 aaaagtacgt gtttgttttg aacaaatacc tgtatcaata gaaggaacaa cgctaataac
     2881 tttctttttc ccttcaaaat cttttaaaga tttttgagat aagtctgtag ctattagtaa
     2941 aaaatctttt gcaatatccc caacttgtag ctgttttcct gtaaaagtaa caggatttcc
     3001 aagaaatgtt gtcataagtt tacctcctta tcaattacct ttattttact atgaagcttc
     3061 ttctttttcg aataattgaa ctgataatta gaggtactta cttccttcgc gtatgctcaa
     3121 agtcgacaac tcatcataat gattactgat aaagtccttt acccatactt tattatattt
     3181 tgaataatcc ctaagcgccc agccaatcgc cttattgata aaaaattctt gacttcctgt
     3241 atttcttaaa ataaaaagtg acaaaatatc agtatttgtt ttttctttaa atcctagttg
     3301 atgctcaata gctagtcgcc tcatccaaaa atcttgatca agtgaccatt ctgacagtaa
     3361 attcataacc ttgtcatctt ttaaggataa gttcccaaaa accttagtca gaacatcaat
     3421 actatcccac cactgttgat gacgagcgta aaactctagt ctagagcaat cattataggt
     3481 aataaattga ctcatagcta atagataatc aagtacaaaa tgatgatatt cacgatagtc
     3541 actttgccaa catttatcaa gcaattgcca atcaattttt gcttgtcttt tagcggcctt
     3601 tataagctct ttgtagagca tacgtcgctc aggtgattta ataccatagc acttaaaatg
     3661 atggcgtaaa taagcctcct gttgcttaga aacttctttg tcacttgcag ctttaaattt
     3721 cctttctaaa cttaggaata aatccattac gtaatcccct tgtatttttc aaaaagcgtt
     3781 gtaattcctt catgtacaga ttctcttgat acacgtaaat tagcgtaagg agtcagttca
     3841 tcaaaatccc ctacttgaac actataatgc gcagcatcaa acatagaaat atcattttta
     3901 tcattgccaa aagctataaa acgattccca aataatttct tagtggcttt tcccttgtca
     3961 actgcaatgg gatttatgta caaacatttt tcaaattcgt ggaaaaagtg actactaagt
     4021 ccaaaacgat ttaatttcgc taacatttgg tctgcccttc ccagctgatc accaaaatac
     4081 aacaccattt ttatcggttt ctcaatttta gaaacttcta gaagctctcc ccttttttga
     4141 ggatcaacat aagcaataaa gggaatttta gaagcctgat aagtagcgta attaaagacc
     4201 tcatcaacaa aatatggaat ctgatagcga tgacaatatg aaataatagt actaaaaaag
     4261 tcactttgta aatagtaact atccactaag tgaccatttt catgtaaagt cgcaccattt
     4321 aaaccaataa tttttgataa agatagcttg tcgccaagga tacctatagt gtctctataa
     4381 gatcgcgccg tagcaaaagt gactctatga ccataatcaa tctgggctct ctagtattcc
     4441 ttgtatttct ttagatagac tcataccatc aaaacaaaga gttccatcta tatcaaagac
     4501 aaaatccatt ctctcaaagt cccatcctta agttaaaata ggttttcata cgtgcataaa
     4561 catcttcatc accaacaaaa aagatatgat ctccttgctc tataactgag aaaggccccg
     4621 gagaaacaat aaatttccct tcgtgttcaa tagccacaat agtcgcacca gtttgatgcc
     4681 atacatttaa ttctcctatt gacttaccaa ggtgttcaga atcttcactg acaataattt
     4741 catatggtgc taatgggtat tgtttactaa ccgcacgtgt ttgaagcaaa aaatcatcaa
     4801 ctaaagtagc taactcttcc atttcttgtt gctgagcctt aatattatca cggattttac
     4861 ctttcaaaat agcaactgaa tgagaggttt catattggtt taagaattca attgcttttt
     4921 ctttagaaag aataatggct ccactgccat gctttaaagt taaaatctgt aaatctgcta
     4981 aaatattaag acccttacgt gctgtttctg gtgatacgtt gaatgttgaa gcaatagttg
     5041 ttctagattt caacttctct ccaacttcat aatctccatt tgcgattctt tgtgcaaccg
     5101 ctactgctat tttttgatat ttagatgtta caatttcaga ttgttcagaa accatccacc
     5161 cctccttatc ctaataaaaa catacatatt aaaccacaat tagcaaaata ttgcaaatat
     5221 atcttttttg ttaatatcta atgtctttag taaatatcat ataaaaggag ctggtctccc
     5281 agctccgagt gtcttatcat aaaaagtagt ttactgaatt aagattcact agtacgagct
     5341 tctgagattt tttcttttgc agcctcagta ccatttgata ctgcttcttt tgttttctct
     5401 gctccagagg aaattgctgc tttagctttg ccagcttgtt cagaagcaaa gtttcctgtt
     5461 gcttgtgctg catcagatac acgatcttgt actgtcactg agtcagcttc atgttcagct
     5521 ttagtcttaa catctacgac atttgcgttc aattcaacaa ctttaaggtg ggtcatcact
     5581 tcaacgtttt gactgacaat cgctttaatg ctttcaacga tagctgggat atccttgccg
     5641 tattcaacaa cgatgtctaa atcaactgca acttctttag taccaacttc aacatttaca
     5701 ccatcagtta ctgagtcaga atttacaacg ctatttttta gattagagaa gaaacctcca
     5761 ctcacagcca agagtccgtc tactgattct aaagcatgtc caacaatttt agcaattacc
     5821 ttttcttcat aagttactga agttttaata tcttgttttt gattttccat aaataaatga
     5881 cctcaaacct tctagtcctt tttcttaaag ctctctgaag cacccttaat ggtatcttta
     5941 atgtcagcaa gtttttcttt tgcatgacct ttcaatttat cgactttacc ttcagattca
     6001 acttctttat cgccagtaag tttcccaaca gattctttta ctgaaccaga aaccttatca
     6061 acttttgcat caaatttttc ttcagacata atagcctcct actaataaca atactttaaa
     6121 ctatacttaa atcaattatt gtacacgtgg ctcagacttc atgtcttcca ctttatcaac
     6181 accgttacct acggcacttt taacgtttga aacttgattc gaagcaaatt cacctgttgc
     6241 ttgtgctgca cttgttacac gatcttgaag agttactgaa tcttcttcat gttgagcacg
     6301 agttttaata tcaacaacat taacattaac ttcaaccact tcaaggtctg tcatacgttt
     6361 cacttcagct tcaacaactt ttttgatatc ggcaaagata gttggaacat gtttttggta
     6421 ttcagcaaca atatccaaat caacagcaac ttgcttttta cccacttcaa catttacgcc
     6481 atctgtaact gaatcagaat ttacaacgct attttttaaa tttgagaaaa aaccaccatt
     6541 tacagccaaa agaccatcta catgctcaat cgcaattcca acaatttttt caatcacttt
     6601 atcttcgaaa gttaattctc cacgaacagc tgtcgtgcca gaattgttag ttgtgttttt
     6661 gatgtaagtt tcagtcataa tagttcctct ttcttttatt taaataatgt agaataaata
     6721 taaacgaaaa attttatttt ctcttattaa agaattggtc aagcatacct gttctttgca
     6781 cataaagccc agcataagca cccagcacaa tgataacaag agcaagaatt gttttaaaga
     6841 atccaatggt tacaatcatt gcagctaaaa cgagcccaat aactgcacct cctagtggat
     6901 atctgtattt cctcacgaat tctgacatat tagactccta ttctacacga cttttattcg
     6961 tcttagcatt cccaacattt tcttcttcaa cttgcttaac aaatactcta gtgttcattt
     7021 tcttttccaa cccaaagaat tcttttaagc caagttcaac atcactttga atatgttcaa
     7081 gttgttctgg tacatttgta cgagaagtta aaagcccttt aacatcaact tttacttttt
     7141 tcttatataa atgagctgtc acgctaggag attttatcaa accagttttt aatacttctg
     7201 ttttaacaaa tccctctatt gcagattttt ttaacatcaa tttaccagtt ttatcagcca
     7261 gtttatactc tgtgtatcgt cttggataaa acagaactac caatatagcc agtaaaacga
     7321 ttacaaacag cactaatgct ccccagaaga aataatgata cataccaacg tcaatcggtg
     7381 atggcaaatt aaaatcccaa tctaaccaat taaaagatgg tagataaatg tgttgtttac
     7441 tgataacagc tacaaatcct agtagggtaa gggagattag acctagtaaa gtatataaac
     7501 tttttagtcc ttttgacata ggtaactcct tttctagtta ttcattttac ctagtacaaa
     7561 tgaagtaaca atgacaacaa taatcgcacc tacgattgaa ggaatcaaag ccatacctgc
     7621 taatttaggc ccccatgttc ctagtaaact ttgaccaaca aatgaaccaa ctaaaccagc
     7681 taagatgtta gctatccaac ccattgagcc acctttgtta gtaacagccc ctgcaatagc
     7741 accgataatc gcacctacga ttaatgacca aattaaactc atgataaata ccttctcttt
     7801 cttgactaat cattccacta gttatgtagt tatgattttt tgcctaatac aaatgaagta
     7861 acaatgataa cgataatcgc acctacgatt gaaggaatca aagccatatc tgctaactta
     7921 ggcccccatg ttcctagtaa actttgacca acaaatgaac caactaaacc agctaagatg
     7981 ttagctatcc aacccattga accacccttg ttagtgattg ctcctgcaat agcaccaata
     8041 atagcaccaa cgattaatga ccaaattaaa cccatgataa atacctttct tcctaagtac
     8101 atctcagtgt acaaacttat tttgttctta actaaataac aagagtttct taagtgacca
     8161 ctcggcaatt aattaagttg ttaactatac taatattata caactttata gagtgactgc
     8221 caagtaaaaa gcttggtaaa
//
LOCUS       DATRTN010000073         8171 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.073, whole genome shotgun sequence.
ACCESSION   DATRTN010000073 DATRTN010000000
VERSION     DATRTN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.073"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            475..726
                     /locus_tag="VBE22_001585"
     CDS             475..726
                     /locus_tag="VBE22_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203028.1"
                     /translation="MMILLSYVLTKTEPISNYQESSSNLKKWLFAIGAFSVLFCWALL
                     VKTFFPIRQNQEGLIADMNQVNHYFRVVLLLTICLIAPI"
     gene            745..999
                     /locus_tag="VBE22_001586"
     CDS             745..999
                     /locus_tag="VBE22_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7203029.1"
                     /translation="MFVMRTFGNSLSGPLVVILSSILFSWSHLHGLSVVDFVVYFGMG
                     LIFASLHHYTKSIHYSIGEHIVWNSLSYIFYFLAFLLDLL"
     gene            complement(1336..1530)
                     /locus_tag="VBE22_001587"
     CDS             complement(1336..1530)
                     /locus_tag="VBE22_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203030.1"
                     /translation="MIMSGQRATSTIQSDINIFHHIEAICISKSNSIGLPAIQNHLGQ
                     LIQRERLGLFFSITYSKAPT"
     gene            1529..1723
                     /locus_tag="VBE22_001588"
     CDS             1529..1723
                     /locus_tag="VBE22_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203031.1"
                     /translation="MLFLMIKPDYIRYFKVIDDMHDTMYQQTVFHVQLQAWKYTMKSI
                     DQLVVDDLNHISNEKTTLVK"
     gene            complement(1774..1950)
                     /locus_tag="VBE22_001589"
     CDS             complement(1774..1950)
                     /locus_tag="VBE22_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203032.1"
                     /translation="MSKKTDSNYLHSNCSDKKIWSDEAPLQQETIPKDRDCVIKPVIT
                     MTVEHVEIPLDDQS"
     gene            complement(1986..3578)
                     /locus_tag="VBE22_001590"
     CDS             complement(1986..3578)
                     /locus_tag="VBE22_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEO7203033.1"
                     /translation="MKIKKIISGLAAALIISSLSTINYEVKADDTTSEYHYISKQNNE
                     EQLISYIKEQHRLLNQFVVDNVNSFTQLNANPTIEQLNRAITLFKQKDEQLFNQVKAG
                     HLSPSNYNAIVNQRNVINQTVQNLIDQNHNKLQTSQNKAAQLVGQRNQVVNKIQAILA
                     TVNYNSVNSIQEAENLFHSLRNQIEPLVAEVNNYKAAMAILQQEVDALSTAAIETETS
                     KLATLKVSENTSVPANKVEEKTTQSEASGNKQEVTKSEEKQATSDAKASQPESANIAD
                     YDSLKEVLRNNISNQVPHISVQMEFKTQEQVDEYQKNLGSIIREIGDTLGTATEFNAK
                     SNISTYTLGGQIQRIIVKSDITITYTLKGDMVGLHKEYKQFVDSFVKENITNKNITSD
                     YEKAKVIHDHLVNNYTYATEELATTRETASGISIHAPEALYKDKRGVCQAFAVMFKDM
                     AATAGLSVWYVTGQAGGGNHAWNIVTINGVKYYVDTTWDNNIKSNKYFLVGKTIMDAD
                     HLLDSQYNALAKDIPADRHQGA"
     gene            complement(3639..3707)
                     /gene="shp2"
                     /locus_tag="VBE22_001591"
     CDS             complement(3639..3707)
                     /gene="shp2"
                     /locus_tag="VBE22_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEO7203034.1"
                     /translation="MKKINKALLFTLIMDILIIVGG"
     gene            3795..4643
                     /gene="rgg2"
                     /locus_tag="VBE22_001592"
     CDS             3795..4643
                     /gene="rgg2"
                     /locus_tag="VBE22_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEO7203035.1"
                     /translation="MEKELGKTLRRLRKGKKVSISSLADEHLSKSQISRFERGESEIT
                     CSRLLNILDKLNITIDEFVSIHSKAHTHFFILLNRVRKYCAEKNVTKLVALLEDHNHK
                     DYEKIMIKALIFSIDQSIEPNQEELARLTDYLFTVEQWGYYEIILLGNCSRLINYNTL
                     FLLTKEMVNSFAYSEQNKTNKILVTQLAINCLIISIDHSYFEHSHYLIDKVRSLLQDE
                     VNFYEKTVFLYVTGYYHLKLGDTSSGKEDMRKALQIFKYLGEDSFYNTYNEHYCQKVL
                     GNNKEC"
     gene            4792..5613
                     /gene="mutM"
                     /locus_tag="VBE22_001593"
     CDS             4792..5613
                     /gene="mutM"
                     /locus_tag="VBE22_001593"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEO7203036.1"
                     /translation="MPELPEVETVRKGLERLVVNQEIASITIKVPKMVKTDLNDFMIS
                     LPGKTIQQVLRRGKYLLFDFGEMVMVSHLRMEGKYLLFPNKVPDNKHFHLYFKLTNGS
                     TLVYQDVRKFGTFELVRKSSLKDYFTQKKLGPEPTADTFQFEPFSKGLANSKKPIKPL
                     LLDQRLVAGLGNIYVDEVLWAAKIHPQRLANQLTESETSLLHKEIIRILTLGIEKGGS
                     TIRTYKNALGEDGTMQKYLQVYGKTGQPCPRCGCLIKKIKVGGRGTHYCPRCQCL"
     gene            5604..6197
                     /locus_tag="VBE22_001594"
     CDS             5604..6197
                     /locus_tag="VBE22_001594"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEO7203037.1"
                     /translation="MLMTKIIGLTGGIASGKSTVTKIIRESGFKVIDADQVVHKLQAK
                     GGKLYQALLEWLGPEILDADGELDRPKLSQMIFANPDNMKTSARLQNSIIRQELACQR
                     DQLKQTEEIFFVDIPLLIEEKYIKWFDEIWLVFVDKEKQLQRLMARNNYSREEAELRL
                     SHQMPLTDKKSFASLIINNNGDLITLKEQILDALQRL"
     gene            6321..7022
                     /locus_tag="VBE22_001595"
     CDS             6321..7022
                     /locus_tag="VBE22_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7203038.1"
                     /translation="MELVIRDIRKRFQETEVLRGASYRFYSGKITGVLGRNGAGKTTL
                     FNILYGDLAADNGTICLLKDNHEYPLTDKDIGIVYSENYLPEFLTGYEFVKFYMDLHP
                     SDDLMTIDDYLDFMEIGQTERHRIIKGYSDGMKSKLSLICLMISKPKVILLDEPLTAV
                     DVVSSIAIKRLLLELSEDHIIILSTHIMALAEDLCDIVAVLDKGKLQTLDIDRKHEQF
                     EERLLQVLKGDEYDK"
     gene            7012..>8171
                     /locus_tag="VBE22_001596"
     CDS             7012..>8171
                     /locus_tag="VBE22_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203039.1"
                     /translation="MTSKALIWSILQIDSARAYNSFLYYLQKIPWLGRKIPNRWFKTD
                     DIKGVVLFIRIIFFFLRGFGRSIGYVLYCYVLSFFVRSGMKQLNILPQEGFTLTLTLM
                     VITSLLIVINFYIKLIEPNDMQQTHMIRLFRIEPSRYFQSAELLEASSELFFRSLSFG
                     IFFHLNHFAFWHGLTFALFLAGSRLGIKVHCLPLYGRGKDQENPESLLNILFYLGIGI
                     GVFLSLFFLLLGKQFSPYPFFSWYTGIAGLIIWLVSYYRLKTYSKLNHLTYLTLTHET
                     MKEKIAKIDNIELLGIEMKDRDINVSELSPLLFENLSGISYLNAIFLKRMSRYLQRKI
                     LVRLAVLSVLFGIELLFLLFFKEKINIPMNEHSFSKFLFLSAVPGYLIYIGES"
BASE COUNT         2611 a         1222 c         1490 g         2848 t
ORIGIN      
        1 aaacttagaa agtgaaatga attatattta aaatttgttt tcaacagagc ggatttgacc
       61 atctgttgtt tgccatgcct ttcataattt ttcagtaatt atgatagttg ttctgttctc
      121 ccaatcataa aactttaaat aagattctat tagttcttgc ctcactctcc ttaagttttt
      181 ggtaaaaatc gaaaagttac cagactttaa aggggagttt tttatttgtc aacagttagt
      241 agaaaaacga tatattgctc catttagatt aattatggag ataaccaaat aatagcttgt
      301 gataagaaga tacctttgaa gtttcagaaa aaatctgttt tccatcctgc tatattcatc
      361 tttgccaaag ttgcaacaac acaataataa caattgcttg ggcattgggt gattagttta
      421 ctatcaatag ggttagagtg gagttggagt atatggattg gcattaatct tataatgatg
      481 attctgttga gttatgtgct aacaaaaact gagccaatca gtaactacca agagtcatca
      541 tctaacctca aaaaatggct atttgcaata ggtgctttta gcgttttatt ttgctgggca
      601 ctactagtaa aaacattttt cccaataagg caaaaccagg agggattaat agctgatatg
      661 aatcaggtca atcactactt tcgagttgtc ttattattga ctatctgtct tattgcccca
      721 atttaagaag agataacgtt caggttgttt gtcatgagaa catttgggaa ctccctgagt
      781 ggtcccttgg ttgtgatctt atcttctata ctatttagct ggtctcactt acatggtcta
      841 tctgtcgtcg actttgttgt ctattttggt atgggtttga ttttcgctag tttgcaccac
      901 tatacaaaat ccattcatta ttcgattggc gagcatattg tttggaatag tttgtcatat
      961 attttttatt ttctagcatt tttgcttgat ttactctaag taataagatt gagagactaa
     1021 tcattactaa gacatttttg tcagtttaaa agagcttttt ggctcttttg ttattcgttt
     1081 ttttaatgaa taaaatgtta ttattttatt gttaactaaa taaagttcgt aactttatat
     1141 gatttatata gaattacgag ttttttgtta gataaatatg agaaaatata gtatgatgaa
     1201 ttcctaaata aaaaataatg aaaggaacat gatgttaaaa gatgttaatg ttggaatgag
     1261 tcatttatta gttagaggtg ctattattct agtagtaggg attgtttttc tgttgcaaca
     1321 gattcagtaa gtacttcaag tgggagcttt ggaataggtt atcgagaaga aaagacccag
     1381 acgttctctt tggatcagct gtcccaagtg attttgtatt gcggggagcc caatactgtt
     1441 agatttactt atacaaatag cttctatatg atgaaagata tttatatcgc tctggatggt
     1501 gctggtagct ctttgaccac tcatgatcat gctgttccta atgattaaac cagattatat
     1561 aaggtatttc aaagtcattg acgatatgca tgataccatg taccaacaga ctgtttttca
     1621 tgttcaactg caagcttgga aatacaccat gaaatcgatt gatcaattag ttgtagatga
     1681 tttgaatcat atcagtaatg agaaaaccac tttggttaaa taaaaaggaa cactgtcaaa
     1741 aatggcagtg ttccttttta ttttaggaga gagctaagat tggtcatcta atgggatttc
     1801 cacatgttca actgtcatag taatgacagg tttgataaca caatctctgt ctttagggat
     1861 agtctcctgt tgcagaggcg cctcatcgct ccaaattttc ttgtcagaac aattggaatg
     1921 tagataattt gaatcagtct ttttagacat ttcatatcct ttccaggctc aatacaattt
     1981 ttgagttatg caccttgatg gcgatcagct ggaatatctt tagctaatgc attgtattga
     2041 ctatccaaaa gatgatcagc atccattatt gttttaccaa caaggaaata tttattgctt
     2101 tttatattat tatcccatgt tgtatcaaca taatatttaa cgccattaat agtaacaatg
     2161 ttccaagcgt gatttccacc tccagcttga ccagttacat accatactga taagccagca
     2221 gtagcagcca tatctttaaa cattactgca aaggcttgac aaacaccacg tttatctttg
     2281 tagagtgctt caggagcatg gatactgata ccactagcag tttcacgagt ggttgccagt
     2341 tcttcagtcg cgtaagtgta attattaacc aagtggtcat gaattacttt agctttttca
     2401 taatcacttg tgatattttt gttagtaata ttttctttga caaaagaatc tacaaactgt
     2461 ttatattctt tatgtaatcc taccatgtca cctttaagag tataggtgat tgtgatgtcg
     2521 ctttttacaa taatgcgttg gatttgtcca ccaagagtat aagtgctaat gttacttttg
     2581 gcattgaatt cagttgctgt tccaagtgta tctccaattt cccggatgat gcttccgaga
     2641 tttttttggt attcgtcaac ttgttcttga gttttaaact ccatttgaac actgatgtgt
     2701 ggtacttggt tgctaatatt atttcgtaaa acttctttta aactatcgta atcggcaata
     2761 ttagctgact caggctgtga tgcctttgca tcagaggtag cctgtttttc ctcactctta
     2821 gttacttctt gtttattgcc tgacgcttct gattgagtag ttttttcttc tactttgttt
     2881 gcaggaacag aagtattttc gctaactttg agagtagcaa gtttagaagt ctcagtttca
     2941 atagccgctg ttgatagggc atctacttct tgttgaagga ttgccatagc agctttgtaa
     3001 ttattaactt cagctacaag aggttcaatt tgatttctga gtgaatgaaa taaattttca
     3061 gcttcttgta tagaattcac agagttgtag tttacagttg ctaaaatagc ttgaattttg
     3121 ttaacaacct gattacgttg ccccacgagc tgagctgctt tattttgact tgtctgaagc
     3181 ttattatgat tttggtcaat cagattttga acagtttggt taatgacatt acgttgatta
     3241 acgatagcat tatagttact gggagagaga tgaccagctt tcacctggtt aaataattgc
     3301 tcatcttttt gtttaaataa tgttatagct ctatttaact gttcaatagt tggattagca
     3361 tttagttgag tgaatgagtt gacattatca acaacaaatt gattgagcaa acgatgttgt
     3421 tccttgatgt aactaataag ctgctcttca ttattttgct tactgatata gtgatactca
     3481 ctggtggtgt catcagcttt aacctcatag ttaatagttg atagtgaact gataattaaa
     3541 gctgcggcaa ggccactaat aattttttta atcttcatta gttctcctgt tataaactaa
     3601 ataaaatggg attccataac aaccatttgt ggggagtttt atccaccaac gataattagg
     3661 atatccatga ttaatgtgaa caacaaagct ttgttaattt ttttcatgac tatctccttt
     3721 ctgattttct attttgtact tgtattatat aatggattta aagggtcttt taaattgttg
     3781 tgaaagtggg aaaaatggaa aaagaattag gaaaaacact aagaagatta cgtaaaggaa
     3841 aaaaagttag tatttcttct ttagcagatg agcacctatc caagtcacaa atctcaaggt
     3901 ttgagagagg agagtcagaa attacttgta gtcgtttgct taatatttta gacaaactca
     3961 acatcactat tgatgaattt gtgtcgattc attctaaggc gcacactcat ttttttatcc
     4021 ttttaaatcg agtaagaaaa tattgtgctg aaaaaaatgt aactaaatta gtggctctct
     4081 tagaggatca taaccataaa gactatgaaa agattatgat taaggctctt attttttcaa
     4141 ttgatcaatc aatagagcct aatcaagaag aattagcacg cttaacagac tatcttttta
     4201 cggtggagca gtggggatat tatgaaatta ttctcttagg aaattgctca cgtctcatta
     4261 attacaatac tttattttta ttgaccaaag aaatggttaa ttcgtttgct tattcagaac
     4321 aaaataaaac caataaaatc ctagttacac aattagcaat taattgtcta atcatcagca
     4381 ttgatcactc ttactttgag catagccact acttaattga taaggttaga tcattgttac
     4441 aggatgaagt taatttctat gaaaaaactg ttttcttata tgtaactgga tactatcatt
     4501 taaaacttgg tgatacttct tcaggaaaag aagatatgag gaaagcttta cagattttta
     4561 aatatttagg tgaagattca ttttacaata cttataatga acattattgt caaaaagtac
     4621 ttggcaataa taaagaatgc taatgagtgc aagtttatga gtaattttta aagcttttgt
     4681 ctcaaaatga taataagaaa ggaaaataga aatcgaatgg tcaggtgaca tcaagaacgt
     4741 ttggaaatta ttgctctaag caatgactat tgatttttag actatatatt tatgccagaa
     4801 ttaccagaag tagaaaccgt tagaaaagga ctagaaagac ttgtagttaa tcaagaaata
     4861 gcttctataa cgattaaagt ccctaaaatg gtgaaaactg atttgaatga ctttatgata
     4921 agtctaccag gaaaaacaat tcaacaggta ttgagacgag ggaagtatct tctttttgat
     4981 tttggtgaaa tggttatggt atcacattta cgcatggaag gaaaatatct actcttccca
     5041 aataaagtac cagataacaa acattttcac ctttatttca aattgactaa tggcagcaca
     5101 ttggtttacc aagatgtccg taaatttggg acatttgagt tggttcgtaa atcatctctc
     5161 aaagattatt ttactcaaaa aaaattaggt cctgagccaa ctgctgacac atttcaattt
     5221 gagccttttt caaaaggctt agcaaactcc aaaaagccaa taaaaccttt actattagat
     5281 cagagattag tagcaggact tggaaatatt tacgtagatg aagtcttgtg ggcagcaaaa
     5341 attcacccac agcgtttggc taatcaattg accgaatctg aaacaagctt attacacaag
     5401 gaaattattc gcattttaac tttaggcatc gaaaaaggtg gctccacgat tagaacatat
     5461 aagaatgctc ttggagagga cggtacgatg caaaagtatt tacaggtcta tggtaaaact
     5521 gggcaaccgt gtccaaggtg tggttgttta attaagaaga taaaagtagg agggagaggg
     5581 acgcattatt gccctcggtg tcaatgctta tgacaaaaat aataggactg acaggaggga
     5641 tagcttctgg aaagtcaacg gtaacaaaaa taatacgaga atcaggtttt aaagtcatag
     5701 atgcggatca agtggttcat aaattgcaag ctaagggtgg gaaactttac caagctttat
     5761 tagaatggtt gggtcccgag atacttgatg ctgatggtga gttggataga ccaaagcttt
     5821 ctcaaatgat ttttgctaat ccagacaata tgaagacatc agctaggcta caaaatagta
     5881 tcattcgtca agagttagca tgtcagcgcg accaattaaa acaaacagaa gagatatttt
     5941 tcgtggatat tcctttattg attgaagaaa agtatataaa atggtttgat gagatttggt
     6001 tggtatttgt tgataaagaa aaacaattac aacgattaat ggcccgtaac aactacagtc
     6061 gagaagaagc agaattacga ctttcacacc aaatgccttt aacagataaa aaaagtttcg
     6121 ctagtcttat tattaataat aatggtgatt taataacttt aaaagagcaa atattggatg
     6181 ctcttcaacg tttatagaac tactaaatgg ataatgtatt tatccatttt ttgtctttat
     6241 tacactagtt ttaaaaaact tgcgattaaa ccacagtaat agtacaatga tataaaaggt
     6301 ttactttcaa aggagttttt atggagttag taattagaga tattcgtaag cggtttcagg
     6361 aaacagaggt cttgagagga gcaagttacc gattttattc aggtaaaata acaggggtct
     6421 taggtaggaa tggtgctggg aaaacaactt tatttaatat actttatggg gatcttgcag
     6481 ctgacaacgg gaccatttgt ttattgaagg ataatcatga gtatcctctt accgataagg
     6541 atattggtat tgtttattcc gaaaactacc ttccagaatt tttaacaggg tatgaatttg
     6601 taaaatttta catggattta catccttcag atgatttaat gacaatagat gattatttag
     6661 attttatgga aataggacaa acagagcgtc atagaattat caaaggatat tctgatggaa
     6721 tgaagagtaa gctctcatta atttgcctga tgatttctaa gccaaaagta attttactag
     6781 atgagccact gactgcagtt gatgttgtat caagtattgc aataaaacgc cttttgttgg
     6841 aattaagtga ggatcatatt attatattat caactcatat aatggcctta gcagaagatc
     6901 tatgtgatat tgtggctgta ttagacaaag gaaaactcca aacattagat attgatcgta
     6961 aacatgaaca attcgaagag cgtcttcttc aagtgttgaa gggagatgaa tatgacaagt
     7021 aaagcattaa tatggtctat attacagatt gatagtgcta gagcttataa tagtttttta
     7081 tattaccttc agaaaatccc ctggttggga agaaaaatcc ctaaccgatg gtttaaaacc
     7141 gatgatataa aaggtgtagt actatttatt cgtatcattt tctttttctt aagaggtttt
     7201 ggtcgttcca taggttacgt actatattgt tatgttttat cattttttgt tagaagtggc
     7261 atgaagcagt taaatatttt accgcaagaa ggttttaccc taactttaac attgatggtg
     7321 attacctctc ttttaattgt cataaatttc tatataaaac ttatagaacc taatgatatg
     7381 caacaaactc atatgattcg ccttttcaga attgagccat cgaggtattt ccaatccgct
     7441 gaacttttag aagccagtag tgaactattt tttagaagtt tgagctttgg catatttttc
     7501 catttaaatc actttgcttt ttggcatggt ttgacttttg ctttattttt agcaggtagt
     7561 cgccttggaa tcaaggtgca ctgtttacct ttatatggta gaggtaaaga ccaagagaat
     7621 ccagagtctt tactaaatat tttattttac ttagggattg gtataggagt cttcctttct
     7681 ctctttttcc ttcttcttgg caaacagttc tctccatatc catttttttc atggtacacc
     7741 ggtattgcag gacttatcat atggttagtt agttactatc gattaaaaac ttattctaaa
     7801 cttaaccact tgacctacct taccctaacg catgagacga tgaaggagaa aattgcaaag
     7861 attgataata tagagctgct tgggattgaa atgaaagata gggatataaa tgtcagtgaa
     7921 ctttctcccc tgctatttga aaatttatca ggtatttcat atttaaacgc tatcttttta
     7981 aaacggatga gtagatactt gcaaaggaaa attttggtac gtttagctgt tttaagtgtt
     8041 ctgtttggaa tagaactcct tttcctgcta ttttttaaag aaaagataaa tattccgatg
     8101 aatgagcata gtttttcaaa attcctattt ttgagtgcag ttccaggata tctaatatat
     8161 atcggtgaat c
//
LOCUS       DATRTN010000074         7956 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.074, whole genome shotgun sequence.
ACCESSION   DATRTN010000074 DATRTN010000000
VERSION     DATRTN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.074"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..241
                     /locus_tag="VBE22_001597"
     CDS             <1..241
                     /locus_tag="VBE22_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEO7203040.1"
                     /translation="FMSGSLIPLTFFPKIVADILGFLPFSSLIYTPVMIIIGKYDGSQ
                     IVQALLLQIFWLIVMVALSQLIWKKVQSHITIQGG"
     gene            243..1028
                     /locus_tag="VBE22_001598"
     CDS             243..1028
                     /locus_tag="VBE22_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7203041.1"
                     /translation="MTKYQRMHFIFIKQYMKQIMEYKIDFFVGVLGVLLTQGLNLLFL
                     NVLFQHIPSLEGWTFQQIAFIYGFSLLPKGIDHLFFDNLWALGQRLIRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGFILLSTTVSSISWTVPKVLLFIFIIPFATLIYTS
                     LKIATSSIAFWTKQSGAVIYIFYMFNDFAKYPVAIYNNLLRWIISFVIPFAFTAYYPA
                     AYFLQDRNVYFNIGGVILISLISFMVSLILWHKGVEVYESAGS"
     gene            1653..1958
                     /locus_tag="VBE22_001599"
     CDS             1653..1958
                     /locus_tag="VBE22_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylX protein"
                     /protein_id="HEO7203042.1"
                     /translation="MGRSNILSRCEQCSDNQCGLYKTRLKDKLLLVVQLVGNIRYSEF
                     LTEGGVYHKGETLLEVITLGISYEYKSYGDIKVIKKYCADGSVVDYNMPILLMERIE"
     gene            1958..2806
                     /locus_tag="VBE22_001600"
     CDS             1958..2806
                     /locus_tag="VBE22_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylD"
                     /protein_id="HEO7203043.1"
                     /translation="MKTVIIYGGQGNVQSKHLCQLVKTPKGEILFSKLQATDLESYQL
                     MMDVINHKVSLDNVHASMLANFLYNDWLTNDEKIVSGIGFSAHSAGIFNVLYASGSTS
                     FENIIHFIKRRAQLIENCHVSEELWLLMVENIDMFSKDVLKEQSEKVKLAIITDDRAG
                     VIAMDQVNRSFFDDVMTNKGYFYKLKQLGVKAPYHTDFLNESKEQYKQLIESLDISQN
                     DDYDYIFHCDQLVDEILYQWTNLFNWRAIKETIIEKDAEIIDISPNRFISSQLQKMKK
                     RGRKQL"
     gene            2803..3525
                     /gene="fabG"
                     /locus_tag="VBE22_001601"
     CDS             2803..3525
                     /gene="fabG"
                     /locus_tag="VBE22_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEO7203044.1"
                     /translation="MKVAIVTGGTRGIGRAITKELYKEGYKVIAIYNSNDAKARALQE
                     ELPKLDVYKCNISDAKAVQKLVTKIFREYGGIDCLVNNAGIVRDGFFLMMSEEKWMDV
                     ININIMGLVNMSKAVLKIMKAKRIQGKVINISSTSGIAGQIGQANYSATKGAIISITK
                     TLAKEFASDGITINCVSPGFIETDMTNELQNKEELKEHLIPLKRFGQPEEVAWLVSFL
                     ASEKANYITGKNIVIDGGMIND"
     gene            3518..3823
                     /locus_tag="VBE22_001602"
     CDS             3518..3823
                     /locus_tag="VBE22_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO7203045.1"
                     /translation="MINWAEERKIIKTRQEVVDEIKGILIDSLMLDIDKDLIMNDQPL
                     FGRGLELDSIDALELSIGISTTFGVELNDDDISILSSVNRLADFVIENSEDIDDQAK"
     gene            3807..4283
                     /gene="fabZ"
                     /locus_tag="VBE22_001603"
     CDS             3807..4283
                     /gene="fabZ"
                     /locus_tag="VBE22_001603"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO7203046.1"
                     /translation="MTKLSSEEIIKMLPHRYPFLMVDRVQSYKKGIIECRKNITINEP
                     YFQGHFPQRPIVPGVLMIEMAAQSAALLYILDALDGSLPSLDELTAEKIAEKVGYLAS
                     VKNFKFKKIVTPGDQLNITCKSQSKLGHLLEIQVVIRDENKKEVASGRMLVSENGN"
     gene            4273..5202
                     /locus_tag="VBE22_001604"
     CDS             4273..5202
                     /locus_tag="VBE22_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7203047.1"
                     /translation="MEIKLKNIGKKYGSFEALRDINLIFEEGKFYGLLGPNGAGKTTL
                     FNLLIQNFKQTSGDIKWEVDGKPLSIKDFYRHIGIVFQSNRLDDNLTVEENLISRGAL
                     YGLSKSQVRNRLKDLQTYLDITAIKKQKYGSLSGGQKRKVDIARALLPQPSLLLLDEP
                     TTGLDPQSRRDLWDAIAQLNQQSQMTVVLITHYLEEMSACDVLNVLIEGNIYYSGDIK
                     SFIEQHSTTNLNVVLKPEKSLDQLSIADFVNKCQVLSEREIVFKDISVEEMMQVISDN
                     QDKSIIETFNVEYSNLEAAYLNLLREKERKNNV"
     gene            5195..6073
                     /locus_tag="VBE22_001605"
     CDS             5195..6073
                     /locus_tag="VBE22_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7203048.1"
                     /translation="MFELISRNRKVYTRDRLAFFMSFLSVIILILVYQIFLGKIQLDA
                     IKAALRSNTVSSDTIHMVNYWLIAGLTTIISMTSTLGAFGVMVSDKEKKLNEDFKVSP
                     ISNIKIELSYAIFAVLFGIVMTMFSCIFAIGIFNGFNSLLEYSAMDYLTILGIVSLGT
                     VLSAAMILPILAFIHTSSAFTTLSTIVGTFIGFISGVYLSIGSVGKALQQVMTWFPLT
                     QINSLLKQVLMKGSIAKVFDKANEATVSNYKESYGVVLRNADGERLSNHFMLIYVIAL
                     ILILLAIHFIIKKVKK"
     gene            6070..>7956
                     /locus_tag="VBE22_001606"
     CDS             6070..>7956
                     /locus_tag="VBE22_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylE protein"
                     /protein_id="HEO7203049.1"
                     /translation="MKDDNKLKISEASLEDYSEVVHLFNRNHVYQFPDGRPLTVDDLD
                     LTLKVKEVTHLFLLKNHGVLIGTSAFFKFITYGCLDWNSSFSGFLLIDSKSRSGQAIT
                     YLYKTILKKITKLKFSNIYTEISNYNKPSLALSKLNGFKEYDKTYEDILHCRSLRSHL
                     PKILNTFRISNYYGKTYDISTFQIMEEIENPLEEETEIRTKVSDEEILFKAEDSASLP
                     YYLKMSLFQMEIARLDNRYVLQVDFLSEQVKRVRVKTGKYHLANLTRAHPSLTLSRFA
                     NYYYIQATVETLYGNIDVQLERRKKHYRDATICLKRTFQGYDLLISPNGSLIFEKQKR
                     KILEDSFLIFSQPLDKKLVVKEEENHITIKCFYQGALIEKIMTFTSDEEITCVYKCNQ
                     KAKEMFPKLLKQTFKLHCQEQLIRDSEGYLVNVPGSYPIEHDDFLRADKFEDRQFHYY
                     LPNEDKMISYSPPGKASNQMQFRPLCLIDTDSLSFPLTYHFRISQASSEEALINLKKQ
                     PIWDSNYQASATDLLKHISNLTLEEEKDYGIKRMIANRKHYPSHKLVLAYNQIVLPKN
                     EIPRDSELYSISFDYRIRGKFVQIRQGEHVKYDNKSYVLENSQQLVLYVASDDKYILI
                     SAKNG"
BASE COUNT         2663 a         1117 c         1500 g         2676 t
ORIGIN      
        1 atttatgtcg gggagcttga ttcctcttac tttttttccg aaaatcgttg ctgatatttt
       61 aggattttta ccattttcat ctttaattta cacaccagta atgattatta ttggtaaata
      121 tgatggcagt caaattgtgc aagccttact tttacaaata ttttggctaa tagtaatggt
      181 agctttatct caattaatat ggaaaaaagt acagtcgcat atcactatcc aaggaggttg
      241 agatgacaaa atatcagcga atgcacttta tttttattaa acaatacatg aagcaaatca
      301 tggagtataa aatagacttt tttgtaggcg ttttaggagt attgttaaca caagggctaa
      361 atcttttatt tctgaatgtt ctttttcaac atatcccttc tttagaagga tggacttttc
      421 agcaaatagc ttttatttat ggtttttctc tcctcccaaa aggaattgat caccttttct
      481 ttgataactt gtgggctctt ggtcaaaggc ttattcgaaa aggagaattt gataagtatt
      541 taaccaggcc tatcagtcct ttatttcatg tcctagttga aacctttcaa gtagatgcat
      601 tgggggagtt attggttggt tttattctgt tatctaccac agtatctagt ataagctgga
      661 ccgtgccaaa agtattatta tttatattta ttatcccctt tgcaactttg atttatacct
      721 ccttgaagat tgctacgtca agtatcgctt tttggaccaa acaatctggt gctgttatct
      781 atatttttta tatgtttaat gactttgcta agtatcctgt cgcaatatat aataatctat
      841 tgcgttggat cattagtttt gtcattccat ttgcttttac agcttattat cccgctgctt
      901 acttcttaca agatagaaat gtctatttta atattggtgg ggttatcttg atttccttga
      961 tttcttttat ggtatcacta atactttggc ataaaggagt agaagtatat gagagtgcgg
     1021 gttcttgatt agtaaggcaa gccattaatt tttttgagtg tttagaagtt ttaggcttac
     1081 taacttagct ttgttagtga tgatatactc cccttatagt gtctagtgaa tttttgtttt
     1141 tttactgtcc actataaggg gagtatatca ggtgaagagg gactattagt gttgagaata
     1201 atgtccctca tagaacaata attaccatat ctttaccaaa ataaagttaa aaacgctctt
     1261 agatgtgctt tctaagagcg ttttttggtc tataaaaggt aagcaaaaag cagatgtcat
     1321 ttatgaaagt gttgacaaag atgaaatact ttgttactat tttaacataa taatgatatt
     1381 ttaattagag tgtgaattta taattaattt ttataatgtt tattttaaat ttaaactaat
     1441 gtttatagca gtgttttact catgattttg tgaattcaac acttaagtca tgacctcatt
     1501 ttaaagagtt tcagacgaca cagaaaggaa gaaaacataa gaaaggcaat gcacggcttg
     1561 tccatagtag tgtttgaagt agatgtttag aaatatttta ttttccaaca gtactgatat
     1621 cagagcgaag atgattagtt gaaggtgata atattggacg ttctaatatt ttgagtaggt
     1681 gtgaacaatg ttctgataat caatgtggct tgtataaaac gcggcttaaa gacaagttac
     1741 tattagtagt ccaactggtt ggaaatatta ggtattctga gtttcttacg gaaggtggtg
     1801 tatatcataa gggagaaacc ttgttggaag ttataacctt aggaatttct tatgaatata
     1861 aaagttatgg ggatatcaaa gtaatcaaga agtactgtgc tgatggcagt gtcgttgatt
     1921 ataacatgcc aatactatta atggaaagga ttgaataatg aaaacagtaa taatatatgg
     1981 aggacagggt aatgttcagt caaaacacct ttgtcaacta gttaaaactc caaaaggtga
     2041 aattcttttt tcaaagttac aagcaactga tttagaatca tatcagttaa tgatggatgt
     2101 cattaaccac aaggtaagtt tagacaatgt tcatgcgtct atgttagcaa attttttata
     2161 taatgattgg ctaacaaacg atgaaaaaat agtatctggg ataggatttt cagctcatag
     2221 cgccggtatt ttcaatgtgt tatatgctag tggtagtact agttttgaaa acattattca
     2281 ctttattaaa cgtcgtgccc aacttataga aaattgtcat gtgtctgaag agctttggct
     2341 tttgatggtt gaaaacattg atatgtttag taaggatgtt ttaaaagaac agtctgagaa
     2401 agtaaaatta gctattatta ctgatgatag agctggtgta attgctatgg atcaagtcaa
     2461 ccgttctttc tttgatgacg tgatgactaa caaaggttat ttttataaat taaagcagtt
     2521 aggcgtaaaa gcaccatatc atactgattt tttaaatgaa tctaaagagc agtataaaca
     2581 attgattgaa agcctagata tttcgcaaaa tgatgactat gactatattt ttcattgtga
     2641 tcaattggta gatgaaattt tatatcagtg gaccaatctc tttaactggc gtgcgattaa
     2701 ggaaactatc atagaaaagg atgcggagat tattgatatt tctcctaata ggtttatttc
     2761 tagtcagttg caaaaaatga aaaagagggg gaggaaacag ttatgaaagt agcaatagtt
     2821 actggtggta ccagaggaat tggaagggct attacaaaag agttatacaa agaaggttac
     2881 aaagtcattg ctatttataa tagcaatgat gctaaggcaa gagctttaca agaggagtta
     2941 ccaaaattag acgtttataa atgcaatatt tcagatgcaa aagcagtaca aaaattggtg
     3001 actaaaattt ttagagaata tggcggtatt gattgtttag ttaataatgc tggtattgtt
     3061 agagatggct ttttcttgat gatgtctgag gaaaaatgga tggatgtcat taacataaat
     3121 attatggggc tagttaacat gagtaaagcc gtgttaaaaa tcatgaaggc taagcgtata
     3181 cagggaaagg ttatcaatat ttcttcaact agtggtatag cagggcagat agggcaagcg
     3241 aactattcag caacgaaagg cgctatcatt tccattacta aaactctagc gaaagaattt
     3301 gcatcagatg gtattacgat taattgtgta agtccaggat tcattgaaac agatatgaca
     3361 aatgaattgc aaaataaaga agagctaaaa gagcatctta ttcctctaaa aaggtttgga
     3421 caacctgaag aggtggcttg gctcgtaagc tttttagcaa gtgagaaggc aaactatatt
     3481 acaggaaaaa atattgtgat tgatggagga atgattaatg attaactggg ctgaagaacg
     3541 taaaataata aaaacacgtc aagaagtagt tgatgaaatt aaaggtattt tgattgactc
     3601 tttaatgttg gatattgata aagatctgat tatgaatgat caaccattat ttggtcgtgg
     3661 attagagcta gactctattg atgccctaga actgtcaata gggatttcaa caacgtttgg
     3721 tgttgaacta aatgatgatg atatttcaat attatcatca gtaaatagac tagcagattt
     3781 tgtgattgaa aatagtgagg atatagatga ccaagctaag tagtgaagaa attataaaaa
     3841 tgttacctca tcgctatccg tttctaatgg tggatagagt gcagagttat aaaaaaggta
     3901 tcattgaatg tcgtaagaat attactatca acgaacctta ctttcaagga cattttccac
     3961 aacgtcctat agttccgggt gttttgatga ttgagatggc agctcaatct gcagctttac
     4021 tttatatttt agatgcactt gatggtagcc tcccctctct tgatgaatta acagcagaga
     4081 aaatagcaga aaaagtaggt tatttagcca gtgtgaagaa tttcaaattc aagaaaattg
     4141 ttactccagg agaccagcta aacattacat gtaaatctca atcaaaattg gggcatcttt
     4201 tagaaattca agtcgttatt agagatgaga ataaaaaaga ggttgcctca ggaaggatgt
     4261 tggtatcaga aaatggaaat taaactcaaa aatattggaa aaaaatatgg ttcttttgaa
     4321 gcattgagag acattaatct catctttgaa gagggaaaat tttatggcct tcttggacct
     4381 aatggagctg gtaaaacaac tttatttaac cttttaattc aaaattttaa gcaaacgtca
     4441 ggcgatatta agtgggaggt ggatggaaaa cctctatcta ttaaagattt ttatcgtcac
     4501 attggtattg tcttccaaag taatcgctta gatgataatt taacggttga ggaaaatcta
     4561 atttctagag gtgctttgta cggtttatct aaatcacaag tacgtaatcg ccttaaagat
     4621 cttcaaacgt atctagatat cacagcaata aaaaaacaaa aatatggcag tttatcagga
     4681 ggtcaaaaac gaaaagttga tattgcacgc gccttgctgc cacaaccttc tctattatta
     4741 ttagatgaac caacgactgg tttagaccct caatctcgtc gcgatttatg ggatgctatc
     4801 gcacaactaa atcaacaatc gcagatgaca gttgtactaa ttactcatta tcttgaagaa
     4861 atgtctgctt gtgatgtttt aaatgttctt attgaaggaa acatttatta ttcgggagat
     4921 attaaaagct ttattgagca acattccaca acaaacttga atgtggtatt gaaaccagag
     4981 aagtcattag accaactttc gatagctgat tttgttaata aatgtcaagt cctttcagaa
     5041 agggagatag tatttaagga tatttctgtt gaggaaatga tgcaggttat ctcagacaat
     5101 caagataaat ctattattga gacatttaat gttgaatatt cgaacttaga ggcggcgtat
     5161 ctcaacttgc ttagagaaaa agaaaggaaa aacaatgttt gaactaattt ctagaaatcg
     5221 taaagtttac acgcgagatc ggttagcgtt tttcatgtct tttttatcag ttattatctt
     5281 aattttggtc tatcagattt tcttgggaaa aattcaatta gatgctataa aagcagcatt
     5341 gaggagtaat acagtatcca gtgatactat tcacatggtt aattattggc tcattgcagg
     5401 tttgacgact attatttcaa tgactagtac attaggtgcc tttggagtta tggtgtccga
     5461 caaagaaaaa aaattaaatg aagattttaa ggtcagtcct atatcaaata tcaaaattga
     5521 attgtcctat gctatatttg ccgttttatt tggtattgtc atgacgatgt tctcttgcat
     5581 ctttgctatc ggtattttta acggctttaa ttccttgcta gagtattcag ctatggatta
     5641 tttaactatt ttagggatcg tttccttagg gactgtttta tctgcggcga tgattttacc
     5701 tattttagcc tttattcata ctagttcagc ttttacgact cttagcacaa ttgtagggac
     5761 ttttattggt tttatttcag gagtatatct ctcaattggc tcggtaggaa aagcattaca
     5821 acaggttatg acatggtttc cattgactca aatcaattct cttttgaaac aggtcttaat
     5881 gaagggttct attgcgaagg tatttgacaa agccaacgaa gccactgtct ctaactataa
     5941 agaatcatat ggtgttgttt tgcgtaatgc tgatggagaa aggctgagta atcactttat
     6001 gttgatttat gtcattgccc tcattcttat tttattggca attcatttta ttattaaaaa
     6061 ggttaaaaaa tgaaagatga taataaatta aagatttctg aagcttcctt agaagattat
     6121 tctgaagtgg ttcatttatt taataggaat catgtttatc aatttccaga cggtaggcct
     6181 ttaactgttg atgaccttga tttaacgtta aaagttaaag aagtgacaca tttattttta
     6241 ctgaaaaatc atggtgtttt gattggaaca tcagctttct ttaaatttat tacctatggt
     6301 tgtttagatt ggaatagtag ctttagtggt tttcttttaa ttgattcgaa aagtcgtagt
     6361 ggacaggcaa tcacttatct atataaaact atcttaaaga agataacaaa actgaagttt
     6421 tctaatatct ataccgaaat cagtaactac aataaaccct ccttagcttt atcaaaattg
     6481 aatggcttca aagagtatga taagacatat gaagacatac tgcattgtcg atcattgcgt
     6541 agtcacctcc ctaagatatt gaatacgttt cgtatttcga attattatgg taaaacatac
     6601 gacatatcaa cttttcaaat tatggaggaa attgaaaatc ccttggaaga ggagacagaa
     6661 attaggacca aagtctctga tgaagaaata ttatttaaag cagaagatag tgcttcactt
     6721 ccttattatt taaaaatgag cttgtttcaa atggaaattg ctaggttaga taaccgttat
     6781 gttttacaag ttgacttttt atcggaacaa gtaaagaggg ttcgggttaa aactggaaaa
     6841 tatcacttgg caaacttaac gagggctcac cctagcctaa ctttaagtcg ttttgccaat
     6901 tattattaca ttcaagcaac tgtggaaacc ctttatggta atatcgatgt tcaactggaa
     6961 cgtcgaaaaa agcattatag agatgcaact atttgtctta agagaacttt tcaaggttat
     7021 gatttactta tctctccaaa tggcagcctt atttttgaaa agcagaaaag aaagatactt
     7081 gaagatagtt ttttgatttt tagccaacct ttggacaaga aattagttgt aaaagaagaa
     7141 gaaaatcaca ttacaatcaa atgtttctat caaggtgctt taattgagaa aatcatgaca
     7201 tttacaagtg acgaagaaat cacttgtgtt tataaatgta atcaaaaggc taaagagatg
     7261 ttcccaaaat tattgaaaca gactttcaag ttacactgcc aagagcagtt gattagagat
     7321 agtgaaggtt atcttgtgaa tgttccagga agttacccga ttgagcatga tgattttctt
     7381 cgagcagata agtttgaaga tagacaattc cattattacc tcccgaatga agataaaatg
     7441 atttcctatt ctcctcctgg caaagccagc aatcaaatgc aatttagacc cttatgtctt
     7501 attgatacag atagcctttc ttttccactg acttatcatt ttaggatttc acaagcatca
     7561 tcggaggagg cgttaatcaa tctaaaaaaa cagccaatat gggacagcaa ctaccaagcg
     7621 tcagcaactg accttttaaa acatatctct aacctaacat tagaagaaga gaaggattat
     7681 ggcatcaaga gaatgatagc taacagaaag cattatccta gtcacaaact ggttttagcc
     7741 tataatcaga ttgttttgcc taagaatgaa attccaagag acagtgaatt atattctatt
     7801 tcttttgatt atcgaattag aggaaagttt gtccaaatcc gccaagggga acatgtcaaa
     7861 tatgataata aatcctatgt tttggaaaat agtcaacaat tagtattgta tgtagctagc
     7921 gatgataaat atatactaat ttctgctaaa aatgga
//
LOCUS       DATRTN010000075         7938 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.075, whole genome shotgun sequence.
ACCESSION   DATRTN010000075 DATRTN010000000
VERSION     DATRTN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.075"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            7..219
                     /locus_tag="VBE22_001607"
     CDS             7..219
                     /locus_tag="VBE22_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEO7203050.1"
                     /translation="MGETSKDLRIPLKISVKQLVMELDSIFSRTERRTKYQLRVINKG
                     ILLGENDILAHHPVTTGDRIRIEEFY"
     gene            219..1489
                     /gene="essB"
                     /locus_tag="VBE22_001608"
                     /pseudo
     CDS             219..1489
                     /gene="essB"
                     /locus_tag="VBE22_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
     gene            1496..2017
                     /locus_tag="VBE22_001609"
     CDS             1496..2017
                     /locus_tag="VBE22_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO7203051.1"
                     /translation="MLVICYYQSLRYEFNIEEEKSFLISSNGKSPIPVLDLENDITLK
                     NIQGQLVYIIDQKEKELTNGVEISGIVFYLANNQKEIYTPLDYEDILIGDKEGYRVRF
                     KEGAPNLLLKKIESNWQLNLFEGDIYLNNHLQKVVQQLPLSLGDEISFQGTIVKLFPD
                     EIQIWGGLIMKHH"
     gene            2029..5904
                     /gene="essC"
                     /locus_tag="VBE22_001610"
     CDS             2029..5904
                     /gene="essC"
                     /locus_tag="VBE22_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HEO7203052.1"
                     /translation="MSAYQFYEGYPDFHRSPRIIYRSSEDKITVNAPGNEPNKSKDEL
                     LKLIVPPLVMIGVSILISIFRPRGIYIIATMSMALVTMIFSITGYFKNRKQYKQDLQE
                     RIDSYHDYLSDKSIELQKLAKEQKRGQHYHYPTIEGLQEMADTYHHRIYEKTPLHFDF
                     LYYRLGLGEVPTSYNIHYSQPERSGKKDPLENEGYNLYFNNRYIKNMPIVANLSHGPV
                     GYIGPRGLVLEQLQLMVNQLAFFHSYHDVQFITIVPEEEMDKWSWMRWLPHETLQDVN
                     VRGFVYNQRSRDQVLNSLNQILKLRRTQREDKSAKEGTLFSPHYVVIVTDEKLILDHV
                     IMEFFTEDPTELGCSLIFVQDVMSSLSENIKTIINIKDRNTGQLVIEEGELKETDFEL
                     DHFLEDYDKENISRRLAPLNHLQNLKSSIPEAVTFMEMYQAEEFEDLHVQERWISHAP
                     YKSSAVPLGLRGQDDIVYLNLHEKAHGPHGLVAGTTGSGKSEIIQSYILSLAVNFHPH
                     DVAFLLIDYKGGGMANLFKDLPHLLGTITNLDGAQSMRALVSINAELKRRQRLFAKAD
                     VNHINQYQKKYKLGEVSEPMPHLFLISDEFAELKSNQPEFMKELVSTARIGRSLGIHL
                     ILATQKPSGVVDDQIWSNSRFKLALKVADRGDSMEMLHTPDAAEITQAGRAYLQVGNN
                     EVYELFQSAWSGADYQPEKDDQGIEDHTIYSINDLGQYEILNDDLSGLDQAENIKEVP
                     TELDAIVENIQALTKEMGISDLPQPWLPPLSNQIAVTDLRKEGSVDLWSKAPSYKAVL
                     GFMDIPSQQAQEVAYHDFEDDGHLSIFAGPSMGKSTALQTVTMDLARHNSPEFLDLYL
                     FDFGTNGLLPLRRLPHVADFFTIDDDEKIAKFIARIKVEMSDRKKALSRYNVATAKLY
                     RQVSGETMPQILIVIDSYEGLREAQTPTNLEACFQNISRDGSSLGISLVISAGRTAAL
                     RSSLMANLKERIALKLTDDSESRTLVGRHQHIMEDIPGRGLIKRDDIEVLQVALSTEG
                     TETFDIINNIQNESDAMNSKWTGPRPKAIPIVPEELTFDDFMATDSVQADLSANRLPL
                     GLEMVDVESYSLALNRFKHMLYMSDSDESLEAVGSHIIKVLLNVPNYHTMIIDSLGEY
                     ESYQGQVRTYVGSDMIEDMADQLIYELQQRESQSEIAEWFIMIPDFESFVAKSNMKLE
                     QVQALLDNGPRYGLHLILGSSYNYVGTKIDAVPKYVRTTTQYVLAGMRLMDQTFLEKV
                     YNSKEIRPNKDEAYIHDRKTYQKLKLTID"
     gene            5918..6268
                     /locus_tag="VBE22_001611"
     CDS             5918..6268
                     /locus_tag="VBE22_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HEO7203053.1"
                     /translation="MAKIQSDASVASTCATAIQSGASGITAVGAPTKDSNSDYSGNSD
                     ASTYIDGEASSSSQIAEKINEFIALIHSTVNEFEAIDQKISQEIAANTKYTMPSNDSS
                     VISNPDFKPKYGLF"
     gene            6280..6642
                     /locus_tag="VBE22_001612"
     CDS             6280..6642
                     /locus_tag="VBE22_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203054.1"
                     /translation="MTERTFEDIELDLKLFQIKLDNAENSKRLLQKLKNDVMELQIEL
                     LESLKLGDAYLTESEELEENNDFILTVNSETLSLEESYDNRINLVSKEIMDYENALDK
                     LYYEKQSLMQKSNERKGG"
     gene            6645..7559
                     /locus_tag="VBE22_001613"
     CDS             6645..7559
                     /locus_tag="VBE22_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203055.1"
                     /translation="MVKVSVSSVGTQASTVAISMFSRVSALNDAITKLSSFAEAATLQ
                     GTAYSNAKSYATGTLTPMLQGMILFSETLSEKCTELQTLYVSICGDEDLDSVVLESKL
                     ASDRASLKIAEALLEHLNDDPEPSKSAISSTKSNIKKLKKRIKSNQKKLDNLNEFNAH
                     SATVFADISNAQSTVNQALAAVSTGFSGYNSKTGAFGKPTSGQMEWTKTVKKNWKERE
                     DAKAEELKSKKAEESKKASKIENTTKKSNVSVDKKKLIKAANEAYKLGEIKKDTYESI
                     ISGLSNASAALLKEVAKSKLTDTARLLM"
     gene            7688..>7938
                     /locus_tag="VBE22_001614"
     CDS             7688..>7938
                     /locus_tag="VBE22_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203056.1"
                     /translation="MKTGAPVIIGSAAKVVGTAAGTATAGLLISTGVLAPPAPVVGSA
                     VDFGATAVIGVGGSMVFDYVYDNKDEINHIIKRASKDTI"
BASE COUNT         2696 a         1400 c         1536 g         2306 t
ORIGIN      
        1 ctagatatgg gggaaacctc aaaagattta cgtatccctt tgaaaattag cgttaagcaa
       61 ttagtaatgg aattagatag tatttttagc cgtactgaaa ggcgtaccaa atatcaacta
      121 agagttataa ataaaggtat tttattggga gagaacgata tattagcgca ccatccagta
      181 acaactggtg atcgtataag gatagaggag ttttattaat ggaaacagat tcgtttgtat
      241 ttgaagaaca agactttcaa tttgaaaaag gaccagattt ttggcagtta tctcttaagc
      301 gttcaaaagt aggaactcaa aatctgcaac aactgcaatt gttagaaatt caccattctt
      361 tgttgatgcc tatgacaact gcaattgatg cagataccat tcaatttcaa ttccagacag
      421 aagcgcacgc tctatttttt gaaagcttta aaaaagaaac tttatctgaa aaattacgat
      481 tagccttgaa tgtcttggat ttagataagg ctttgtcttt gtcagtaaat ttcattttgc
      541 atccctctaa tctattttta acaaaaaatg caactgctaa aatagcttat cgtagccttc
      601 ctgggattat gagacctgag aaatttggtc cagaagagtt tttatatcaa ttcaaatgtt
      661 ttgtctttgc attattgacg caacatgact atatagagtt gtataatggt gctatttctg
      721 ttattgaagt atcagatttc ctaaaaagca tttatcatgc agaaactatt caggctgtta
      781 gagacatcat tactattgat tatgagcagc aagtagaagt agaaactcat acgttagcaa
      841 aagtatcaag ggcaaaatat aagctttata aatacataag tgtttggcta ggagctttat
      901 ctacaatact cttgattccg ctagtatatc tggtttttat tcacaatcca ttcaaagaaa
      961 aaatgttggc agcggatact tcatttatta aagttgatta taatcaagtg attaatcgac
     1021 tagaacatgt aaaagtaagt taccttatac acagaagtac gagttggcct attcctatat
     1081 taatggaatg tcattttctg aagaacagcg tgaagttatt ttaaacaatg ttacgctcaa
     1141 gactgatgag ctctatcttg attattggat taatattggc cgtggtttag atgatgatgc
     1201 tattgatgct gccaaacgtt tagacgattc tgaccttgtt atttatgcta ttgttcagaa
     1261 aatggatcag gttagaaaag acaatagttt atctggtaaa gatcgtgaac aaaaactttc
     1321 tgagctacaa acagactatg ataaatattg gaaagaccgt aagacagcct taacagatga
     1381 ggaatccaaa tcgaaaaata gcaataatca ttcgacaaat tccaacaaag agtcatctga
     1441 atcatcaagt actacagcaa gtacatcttc taaaactaaa agtaggtagg tggatatgtt
     1501 agttatttgt tattatcaat cactccgcta cgaatttaat attgaagagg agaaatcctt
     1561 tctaatatca tcaaacggca agtcgccaat accagtatta gatttagaaa atgacatcac
     1621 gctaaaaaat atacaaggtc aattggtata tattattgac caaaaagaaa aagagttaac
     1681 taatggtgta gagatttcag gaattgtctt ctatttagcg aataatcaaa aagaaattta
     1741 tactccctta gattacgaag atatattaat cggtgataaa gaagggtatc gtgtccgttt
     1801 taaagaagga gcccctaatt tattactgaa aaaaatagaa agtaattggc agcttaattt
     1861 atttgaaggt gatatttatc tgaataatca tcttcaaaaa gtagtgcagc agttaccttt
     1921 aagtttaggt gatgagatta gcttccaagg aacaattgtt aagcttttcc cagatgaaat
     1981 tcaaatctgg ggggggctga ttatgaaaca tcattaacta agaaagttat gtcagcttat
     2041 caattttacg aaggctatcc tgattttcac cgctcacctc gtattattta tcgttccagt
     2101 gaagataaaa ttacagttaa tgcgcctggt aatgaaccta ataagtctaa agatgaactg
     2161 ttaaaattaa ttgttcctcc tctagtgatg attggagtca gcattcttat ttctattttt
     2221 cgtcctcgtg gtatctatat cattgcaaca atgtcaatgg ctctagtgac gatgattttc
     2281 tctatcactg gttactttaa aaatcgtaaa cagtataagc aggacttaca agaacgcatt
     2341 gattcctatc atgactattt atctgataag tcaattgaac ttcagaagtt agctaaagag
     2401 caaaagcgtg ggcaacacta tcattaccca acgattgaag gtttacagga aatggctgat
     2461 acttatcacc atcgtattta tgaaaagaca ccacttcatt ttgacttctt gtattatcgc
     2521 ctaggtttgg gagaagtacc aacgagctat aatattcatt attctcaacc agaacgttct
     2581 gggaaaaaag atcctttaga gaacgaaggc tataacctct actttaacaa tcgttatatt
     2641 aaaaatatgc cgattgttgc gaatttatca catggaccag ttggctatat aggaccacga
     2701 ggtttagtat tggagcaact ccaattgatg gttaatcaat tagctttttt ccattcttac
     2761 catgatgttc aatttattac tattgttcca gaggaagaga tggataagtg gtcttggatg
     2821 cgttggttgc cacatgaaac cttacaagat gtcaatgttc gtggctttgt ttataatcaa
     2881 agaagccgtg atcaagtgct caacagtctt aaccaaattt taaaattacg tcgcacacaa
     2941 cgcgaagata aatctgccaa agaagggact ttattttctc cgcattatgt cgttattgta
     3001 actgatgaaa agttaatttt agatcacgtc atcatggaat tctttacgga agatcctaca
     3061 gaattgggat gttctctcat ctttgttcaa gatgttatga gttcactttc agaaaatatc
     3121 aaaaccatta ttaatattaa agaccgtaat actggtcagt tagttataga agaaggagag
     3181 cttaaagaaa cagattttga attagatcat ttcttagagg attatgacaa ggaaaatatt
     3241 tcacgtcgcc tagctccgtt aaatcaccta caaaatctta aatcttcaat accagaagca
     3301 gttactttta tggaaatgta tcaagcagaa gaatttgaag atttgcatgt tcaagagcgt
     3361 tggataagcc atgctccgta taaaagctca gctgttccac ttggcttacg tggtcaggat
     3421 gatattgttt atctaaacct acatgaaaaa gctcatggtc ctcatggatt agttgcagga
     3481 acaacgggat cagggaagtc agaaattata cagtcctata tcctttcttt agctgttaat
     3541 ttccatccac acgatgtagc tttccttctc atagactaca aaggtggggg gatggcaaac
     3601 ctctttaagg acctgccaca cttacttggc accatcacca acttagatgg tgcccaatcg
     3661 atgcgtgctt tggtttctat taatgctgaa ttgaaacgtc gtcaacgttt atttgctaaa
     3721 gctgatgtta atcatattaa ccagtatcag aagaaataca aacttggaga agtttcagaa
     3781 ccaatgcctc atctcttctt aatctccgat gagtttgccg aattgaaatc caaccaacct
     3841 gagtttatga aagagcttgt ctcaacagcg cgaattgggc gttccctagg gattcactta
     3901 atcctagcga cccaaaaacc atctggtgtg gtagatgacc aaatctggtc aaactcacgc
     3961 ttcaaactag ctcttaaagt agcagaccgc ggcgactcca tggaaatgct ccatactcca
     4021 gatgccgccg agattacaca agcaggacgt gcctacctcc aagttggtaa taatgaagtc
     4081 tatgaactct tccaatcagc atggtcagga gcagattacc aacctgaaaa agacgaccaa
     4141 gggattgaag accataccat ttattccatc aacgaccttg gccaatacga aatcctaaat
     4201 gatgatcttt caggcttaga ccaagccgaa aatattaaag aagtacctac ggaattagat
     4261 gctatcgttg aaaacattca agctttaacc aaagaaatgg gcatctcaga cttacctcaa
     4321 ccgtggctac cgcctctaag caatcagatt gcagtaaccg atctccgtaa ggagggatca
     4381 gtagacctct ggagcaaggc cccatcttac aaagctgttc ttggctttat ggatatacca
     4441 agccaacaag cacaagaagt ggcttaccac gattttgaag acgatgggca tttgtcaatt
     4501 tttgcaggac caagtatggg gaaatcaaca gcccttcaaa ctgtaacaat ggacttagca
     4561 cgtcataata gtccagaatt tttagacctt tacctctttg attttggtac caatggcctc
     4621 ttacctctaa gacgtttgcc acatgtagca gatttcttta ccattgatga tgacgaaaaa
     4681 atagccaagt ttattgcccg tatcaaagtt gaaatgtctg atcgtaaaaa agccctcagt
     4741 cgctacaatg tcgcgacagc aaaactttac cgtcaggtat ctggggaaac catgcctcaa
     4801 atcctgattg ttattgacag ttatgaaggg ctacgggaag ctcaaacacc gactaactta
     4861 gaagcttgtt tccaaaatat ttcgcgtgat gggtcgtcac ttggcatttc cttagttatc
     4921 tcagctggtc gtacggcagc tttgaggtcg tccttaatgg caaaccttaa agaacgtatc
     4981 gctcttaaat taacagatga ctctgaatca cggacacttg tcggtcgtca ccaacacatc
     5041 atggaagata ttccaggacg tggattaatc aaacgtgatg acattgaagt gttacaagtg
     5101 gctctctcaa cagaaggcac tgaaaccttt gacatcatca ataacatcca aaacgaatct
     5161 gacgccatga acagcaaatg gactggacca agaccaaaag caataccaat tgttcccgaa
     5221 gaattaacct ttgacgactt tatggctaca gatagtgttc aagctgacct atcagctaat
     5281 cgtttaccac ttgggcttga aatggttgac gttgaaagtt atagtttagc acttaatcgc
     5341 tttaagcata tgctttacat gtctgattct gacgagtcac tcgaagcagt tggttcccat
     5401 atcatcaagg tcttgcttaa cgtcccaaat taccatacca tgatcatcga ttcgcttggc
     5461 gaatatgaaa gctatcaagg tcaggtacgt acttatgtgg gttctgatat gattgaagat
     5521 atggcggatc agttgatcta tgagttgcag caacgtgaaa gccaatctga gatagctgaa
     5581 tggtttatca tgattccaga ttttgaaagc tttgtggcta agagcaatat gaagcttgaa
     5641 caggttcagg cattgcttga caatggacca cgctatggtt tacatctgat tcttggatca
     5701 agctataact atgttgggac taagatcgat gctgtgccta aatacgttcg cacaacgacc
     5761 cagtatgtgc tggctggcat gaggctcatg gatcaaacct tccttgaaaa agtgtataat
     5821 agtaaggaaa tccgtccaaa caaagacgaa gcctacattc acgatagaaa aacctatcaa
     5881 aaattaaaac tcaccataga ctaagaaagg agaccaaatg gcaaaaatac aaagtgatgc
     5941 ctccgtcgca tccacctgtg caactgcaat acaatcagga gccagtggaa tcacagcagt
     6001 aggagctcca actaaggatt caaacagtga ttatagtggt aatagcgatg cttcaactta
     6061 catcgatgga gaggcaagta gtagtagcca aattgctgaa aaaataaatg agttcatagc
     6121 tctcattcat tctacggtga atgagtttga agctattgac caaaaaatta gccaagagat
     6181 tgcggcaaat actaaatata caatgcctag taatgacagc tctgttatat caaatccgga
     6241 ttttaagcct aaatatggtt tgttttaata ggtgatgtta tgactgaacg aacattcgaa
     6301 gatattgaac ttgacttaaa gttattccaa ataaagcttg ataatgctga gaatagtaaa
     6361 aggctcttac aaaaattgaa aaacgacgtc atggagttac aaatagagtt actagaatca
     6421 ttaaaactcg gtgatgccta tttaacagaa tcagaagaat tagaagagaa taatgacttt
     6481 attctaacgg taaatagtga aacactaagt ttagaagaat cctatgacaa taggataaac
     6541 ttagtttcta aagagattat ggattatgaa aatgcattag ataagttgta ttatgaaaaa
     6601 cagagtttga tgcaaaaaag taatgaaaga aaaggaggtt aaaaatggta aaagttagtg
     6661 taagttctgt aggaactcaa gcatcaacag tagctatttc tatgtttagt cgtgtatcgg
     6721 ctttaaatga tgcaataaca aaactatcat cttttgcaga ggctgcaact cttcaaggga
     6781 ctgcttattc aaatgcaaaa agctatgcta ctggaacgtt aactccgatg cttcaaggaa
     6841 tgattctttt ctctgaaaca ttgagtgaga aatgtacaga attacaaacc ttatatgtct
     6901 caatttgtgg tgatgaggat ttagactctg tcgttttaga atcaaaatta gcaagtgata
     6961 gggcatcatt aaagattgct gaagcacttt tagagcatct taacgatgat ccagaacctt
     7021 ccaaatctgc cataagttct acaaaaagta atattaaaaa attaaaaaaa cgtataaaat
     7081 ctaatcaaaa gaaattagac aaccttaatg aatttaacgc ccattcagca acagtatttg
     7141 cggacatttc taatgcacag tcaactgtta accaagcact agcggctgtt tcaacaggat
     7201 tttctggata taatagtaaa accggagctt ttggaaaacc aacatccgga cagatggaat
     7261 ggacaaagac agttaagaag aattggaaag agcgagaaga cgccaaagct gaagaactga
     7321 aaagtaaaaa ggctgaagaa agtaagaaag cttcaaaaat tgaaaatact actaaaaaaa
     7381 gtaatgtttc agttgataaa aagaaattaa taaaagcggc taatgaagcg tataaattag
     7441 gagaaattaa aaaagatacc tatgaatcaa ttatcagtgg tttaagtaat gcatcggctg
     7501 ccttacttaa agaggtagct aaatcaaaat tgactgacac agctcggcta ttgatgtaaa
     7561 tccttcttac gctaacttca ttagaggctc gagccaattt gtaaaatatg gagcgcctgt
     7621 acttggggct gcaattgact ttggagcagc tcaagtaaaa ggtggagaag atgtagaaga
     7681 tgcaactatg aagacaggag cacccgtaat aattggtagt gctgcaaaag ttgtaggaac
     7741 ggctgcgggt accgcaacag ctggactatt aatttcaaca ggtgtacttg caccgcctgc
     7801 tcctgttgtt ggttctgctg tggactttgg tgctacagca gtaataggtg taggtggatc
     7861 gatggtgttc gattatgttt atgataataa agatgaaata aatcatatta taaaaagagc
     7921 atcgaaggat acaattaa
//
LOCUS       DATRTN010000076         7807 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.076, whole genome shotgun sequence.
ACCESSION   DATRTN010000076 DATRTN010000000
VERSION     DATRTN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.076"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..1292)
                     /locus_tag="VBE22_001615"
     CDS             complement(<1..1292)
                     /locus_tag="VBE22_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7203057.1"
                     /translation="MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVG
                     RDMGTAATVLFLGVIVLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVAS
                     LELFIIYIFLISIGSLFSAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIF
                     LTLEVPVIRHVHLSSPLSLFRKKQQGEKEPKGNLIFAILALVAIAIAYTMALTSGKAP
                     ALAVIYRFFFAVLLVIAGTYLFYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMK
                     QNAVGLASITLLAVMALVTIATTVSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFE
                     EKILKKLGKSSKEAITYNQTMISMPVSQSSELNITSKNVKHVDITKTGFMYLITQNDF
                     RRLGHRLPKLKDNQVAYFVQKGDSRLKKINLLGNKFDVV"
     gene            complement(1294..2046)
                     /locus_tag="VBE22_001616"
     CDS             complement(1294..2046)
                     /locus_tag="VBE22_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7203058.1"
                     /translation="MLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESG
                     SGKTTLLNILATLEKPTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLS
                     VRDNIYLPLVLDRKRYKEMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLI
                     TNPQILLADEPTAALDYRNSEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYRGNKNNSEFNKDISLTMSAILGGE"
     gene            complement(2072..3033)
                     /locus_tag="VBE22_001617"
                     /pseudo
     CDS             complement(2072..3033)
                     /locus_tag="VBE22_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253131.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
     gene            3199..3737
                     /locus_tag="VBE22_001618"
                     /pseudo
     CDS             3199..3737
                     /locus_tag="VBE22_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768524.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
     gene            complement(3717..4565)
                     /locus_tag="VBE22_001619"
     CDS             complement(3717..4565)
                     /locus_tag="VBE22_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO7203059.1"
                     /translation="MKIKKLLGLTTTVVISALILGACGQSKNEDAKVVRVGTMVKSKT
                     EKARWDKIEELVKKKGVKLKFTEFTDYTQPNKALESDEIDINAFQHYNYLNNWNKANK
                     TNLVSVAETYFTSFRLYSGTKNGKGKYQTVSEIPNKATITIPNDAVNESRSLYLLQSA
                     GLLKLKVSGDTLATMSDVVSNPKSLDLKEVDAAQTARSLDSTDAAVINNDFVTEAGIS
                     PKSAIFIEPKSKNAKQWYNLLVAQKGWQDKSKAKAIKEVVKAYHTDAVKKVIEKTSQG
                     LDQPVW"
     gene            complement(4698..5441)
                     /locus_tag="VBE22_001620"
     CDS             complement(4698..5441)
                     /locus_tag="VBE22_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7203060.1"
                     /translation="MHYKELLESSEKPYLETNRYKHFKNIITSERYFSNYISYKIMPT
                     VSEFLQDMIYLADEQKDYQSDFAFVFFEENAELSSELQRTLSQQGFESEKHIIFTNSL
                     NHLKLFEKEFDDITFTKLNENYLEKYLKFKYEQSIVYGETYAKQMYNDNRANLLNNNA
                     KVYLAIKDNQIIGDVTAWDYGEYVEIDDFYVQESYRGQGIGTRLQFDASRGFKKVILI
                     SEEENRKMYEHQGYEEVAYYWTALRSPRK"
     gene            complement(5519..6853)
                     /gene="trmFO"
                     /locus_tag="VBE22_001621"
     CDS             complement(5519..6853)
                     /gene="trmFO"
                     /locus_tag="VBE22_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEO7203061.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEAHRVPAGGAMAVDREGY
                     SEAVTEEIHKHPLIEVIRDEITDIPGDAITVIATGPLTSDSLAAKIHELNGGDGFYFY
                     DAAAPIVDKNTIDMNKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLDQT
                     FATRKNPNLFFAGQMTGVEGYVESAASGLVAGINAARRFNGESEVVFPQTTAIGALPH
                     YITHTDSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIATRALKDLEKFLNY"
     gene            complement(6967..7665)
                     /locus_tag="VBE22_001622"
     CDS             complement(6967..7665)
                     /locus_tag="VBE22_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO7203062.1"
                     /translation="MLPAYIKIHDAIKKEIDKGTWKIGQRLPSERDLADDYSVSRMTL
                     RQSITLLVEEGILERRVGSGTYVASHRVQEKMRGTTSFTEIVNSQGRKPSSKLISFQR
                     KLANETEIQKLNLSQSDYVVRMERVRYADKVPLVYEIASIPENLIKGFEQSEVTEHFF
                     KTLTENGYEIGKSQQTIYARNASERVASHLEVNAGHAILALTQVSYFTDGKPFEYVHG
                     QYVGDRFEFYLENN"
BASE COUNT         2496 a         1440 c         1187 g         2684 t
ORIGIN      
        1 acaacgtcaa atttatttcc caatagattt atttttttca aacgactatc acctttttga
       61 acaaagtaag ccacttggtt atcttttaat tttggtaatc ggtgacctaa acgacggaaa
      121 tcattctgag taattaggta cataaaaccc gtttttgtga tatcaacatg tttgacgttt
      181 ttagatgtga tattaagttc acttgattga ctaactggca ttgaaatcat tgtctgatta
      241 taagtgatag cttccttaga tgacttacct agtttcttca aaatcttttc ttcaaatata
      301 tttttcgcgt caccttttga attatctatt gataaactta ctgattttgg aaatagtccg
      361 gtaacaacat tttgtgtatt tgaatagagt gagactgttg tagcaatagt aactagagcc
      421 ataacagcta ataaagtgat acttgccaac cccactgcat tttgcttcat tcgaaaaatc
      481 atttgcgaag ttgatacaaa atgctcagat ttataataat aatgcttgtt ttgacgtaac
      541 ctttttaagt accatgtcat aaaactaata taaaaaagat aagtaccagc aataactaaa
      601 agtactgcaa agaagaaacg atagataaca gctaatgcag gtgctttacc tgaagtaaga
      661 gccattgtat aagcgatggc gatagctact aacgctaaaa ttgcaaatat aagattacct
      721 tttggttctt tttctccctg ttgtttcttc ctaaagagac ttaatgggga tgataaatga
      781 acatgtcgaa taactggaac ttctaaagtc agaaaaatac ctgtaaatat aacaatacaa
      841 ataataaatg gccataaact taaactaaga tttagtgcat gatagttaat aatgttgaca
      901 aaaattaaat aaataaattt agcaaaaaaa gcactaaaca aacttcctat agaaataaga
      961 aatatataaa taataaacag ctctagacta gctacacgcg caacttgacg tttattcatc
     1021 cccaaaatgt tatacagtcc aaattcacta ctacgctgtt tcatcaagat attgtagcta
     1081 taatgttcca tgactaccgc aaagattgac aaaacaatca ctccaagaaa aagaaccgtt
     1141 gccgctgtcc ccatatctct tccaacagca ctcattaaga ttagtagcgt agaacaagtc
     1201 aatgaataaa gtaataaact ggctaagagg aatggtatgt actggtctat agaatgtttt
     1261 aaattatgcc aggctatttt gagataaaac atattattca ccccctaata tggcagacat
     1321 tgttaaagaa atatctttat tgaattcgct attattctta ttaccacgat aaagttggtg
     1381 aaaaatacgt ccatctttga taaataaaac gcgtttggca tgactagcag catttgctga
     1441 atgggttacc atcaaaatag tttgtccatc caagttaata gtttcaaata aattcagcaa
     1501 gtcttctgaa ttacggtaat ctaacgctgc tgttggttca tctgctaata aaatttgagg
     1561 gttggttatc aaactacgag caatcgctac acgttgcttt tgaccaccag aaagctcgaa
     1621 aggtctctta tctaataagt catcaattct cagatgagaa gataattctg acaaacgatg
     1681 atccatttct ttgtaacgtt tacgatcaag aactaaagga agatagatat tatctcttac
     1741 agagagagta tccaaaaggt tgaagtcctg aaagacgaaa ccaagatttt tcaaacgaaa
     1801 actcgctaat tttgcctctt taattttcgt aatatcttcc ccatttaaaa tcacttgtcc
     1861 atttgtcggt ttttctaaag tagctaaaat atttagcaga gttgtttttc ccgaaccaga
     1921 ctctcccata atagcaatga attccccttg ttcaacttta aaatcgacgt cttgtagagc
     1981 ccttgtctct tcttttgaaa aacgtgtacg aaaaactttt tctaagtgat tgatttctaa
     2041 taacatataa ttctccttta tttattatac cctaattttg attgattcta ctctttaacc
     2101 aagactgtga ctcttgtaaa ggaaggctgg ttacttgatc tggtaagata ggggtgttct
     2161 cgtatttata tccttgtcga tcactaacta ttcctgtagt taaagctaat ctagcaccgt
     2221 cgtaaagcat gaaagtttca ttaacagtcg tatatcctgc tgtggcttga ccaaagcttt
     2281 taacattagg tatcctttaa atgataaaaa agtcatttct gccgaactag cagttttatg
     2341 attagttata atagcgattg gaacatgctt agtattgatt gttttcctag atatttttaa
     2401 tgcttctagc ggtatatttt tcatcgtgat tgttttctta tttccatact tatcagtata
     2461 atgaaatagg gtatcatttg gtaaaataga ggctaacccc ccaatcatag ggatcatatt
     2521 gccgccagta ttattcgata agtctaaaat gagtccctta taactttttt ccatcaatat
     2581 attttgtaat ttaccactat aatggctaat agatcgttta tctaacttac ttatagaagg
     2641 aatagtaatt aagagaatgt cttcgtttaa tcttcgatat tctggatatc gactatctag
     2701 ggtatctctt cgtacagtct ctttggataa tatttgacta tgtttacctc ctccataccg
     2761 tactgcttcc tgtacaatgt tgcgactctc ttggtaagat tttgacgttt tcaacttttt
     2821 ttctgatcgt tgtcttataa tttgccattg acgaccttgc gaatacaaac cacgctgttc
     2881 aacacgatct aaaataacac gtccataacg ttcgctacta ggaggtaata gataaatatt
     2941 taatgctgaa ccataataat gaactaccac tcctaaaatt cctaaaacta taagcattgg
     3001 aactacaaaa agtaaaacta tttttcgccg catctcttgc tccccctttc tctttaagtc
     3061 tattatatgg caaaatagat tactataacc ttacatctta agacctattt cttacaaatt
     3121 tgaaagtttt aaaagcttta taaactataa ttttgatata atatcttaca taacaaatgg
     3181 aaagtacggt gaaaatatat ggccaagaca agtaaaaaat tgacaaaatc tttagggcct
     3241 attggtaaat taatttcaat aatccctgac acaactgaac ttattggaaa agcaattgat
     3301 aattctcgtc caattattga aaaagaactt gatcgtcgtc atgaaaaaaa cagacttata
     3361 ctcagcttga taatgtagta caccttgata tcaatgatgc gcaagtttac ttagagcaac
     3421 gccattttaa tgtatcaagg attgttgctg aaccaaattc aaagtgggca accctacatc
     3481 ctaatcaagt tgtgaatatg tcgcctaaag ctggtagggt cagaatcggt tcattagtga
     3541 agttgtacta tattacagag gatatccttg aagaaagtaa gacactactt ttatctaaat
     3601 ccgaaaaaag taaagcacga caacaacggt taacagaagt attgagtacc cctagtcaat
     3661 tactcaaaaa aatcgaaata aataagcatg atcgaattga ccatgcttat ttatttttac
     3721 caaacaggtt gatctaaacc ttgagatgtt ttttcgataa cttttttaac cgcatctgta
     3781 tggtaagctt tcacaacttc tttaatggct ttagcttttg atttgtcttg ccaacctttt
     3841 tgagcaacta aaaggttgta ccattgtttt gcatttttac tttttggttc aataaaaata
     3901 gctgatttag gacttattcc tgcttctgtc acaaaatcat tattaataac tgctgcatct
     3961 gtggaatcca aagaacgtgc ggtctgtgct gcatctactt cttttaaatc taaagattta
     4021 ggattggaaa caacatctga cattgttgct aatgtatcac ctgatacttt caattttagc
     4081 aagcctgctg attgtaacaa gtagagagag cgactttcgt taactgcatc gtttgggata
     4141 gtaatagttg ctttatttgg aatttcagaa actgtttggt atttaccttt accgttctta
     4201 gtaccagagt ataatctaaa ggaagtaaag tatgtctcag caacggaaac aagattggtc
     4261 ttatttgctt tattccaatt gtttaagtaa ttataatgct ggaaagcatt aatatcaatc
     4321 tcatcacttt ctaaagcttt attaggttgt gtataatctg tgaattccgt aaattttagc
     4381 ttaacgcctt ttttcttaac taattcttcg attttatccc aacgagcttt ttcagtttta
     4441 ctctttacca ttgttccaac tctaacgact ttggcgtctt catttttaga ctgaccacag
     4501 gctcctagaa taagtgctga aattactact gttgtcgtta atcctaatag ctttttgatt
     4561 ttcataattt atttctcctt tgatatggtt gtatgattaa tcaactgata ttagtgtaac
     4621 aaaaagctaa tggataaaaa aatacttatt atcaatcaag agatatagct tttaactata
     4681 tcatttcttt tataatttta ctttcgaggg ctccgtaaag cggtccaata ataggcgact
     4741 tcctcataac cttggtgctc atacattttt ctgttttctt cttctgaaat taagataact
     4801 ttcttaaacc ccctacttgc atcaaattgt aggcgcgtac caattccttg tcctctgtag
     4861 gattcttgga cataaaaatc atctatttcc acgtattcac catagtccca agctgttaca
     4921 tctccaataa tttgattatc tttaatagct aggtaaactt tagcattatt atttaaaaga
     4981 ttagcacgat tatcattata catttgctta gcgtaagttt ctccgtaaac tatactttgt
     5041 tcatatttga actttaggta tttttctagg taattttcat taagtttagt aaatgtgatg
     5101 tcatcaaatt ctttttcaaa aagtttaaga tgatttaatg aatttgtaaa tatgatatgt
     5161 ttctctgatt caaagccttg ttgtgataaa gttctctgta attcactgct caattcagca
     5221 ttttcctcaa aaaacacaaa agcaaaatca gactgataat ctttttgttc atctgctaaa
     5281 tagatcatat cctgtaaaaa ctcacttact gtaggcatta ttttgtagct aatatagttt
     5341 gaaaaataac gctctgaagt gattatattc ttaaaatgtt tataccgatt agtttccaaa
     5401 taaggttttt cggatgactc aagtaattct ttatagtgca tatttactcc attcatacat
     5461 ttttaaaaat caatatagaa aatttgacct tgcttgttgg caaagtcaaa tgaatatatc
     5521 aatagttgag aaatttttct aaatctttca aagctcgtgt agcaatagct tcataacgct
     5581 ctttcttatc acggattcgt gggccttcta attctttgat gataccaaag ttaacattca
     5641 ttggttggaa atgtttgctg tctgtatgtg taatataatg tggcagtgca ccaatagcag
     5701 tagtttgtgg gaaaactact tcactctccc cattaaagcg acgggcagca ttgattcccg
     5761 ctacaagacc acttgctgcc gattctacat aaccttcaac accggtcatt tgtccagcaa
     5821 agaaaagatt tgggttctta cgtgtagcaa aggtttggtc taaaagattt ggtgaatcca
     5881 tataagaatt gcgatgcata acaccatacc ggacaaattc agcattttca agaccaggaa
     5941 tcatttgaaa tacacgtttt tgttctcccc acttaagatg agtttggaaa ccaactatgt
     6001 tataaagaga gcctgcagca ttatcttgac gtaattgaac aacagcatat ggtgttttaa
     6061 attcaccatc tcgcggtcct ttgtagtcct caggatactc caaaccaact ggtttcattg
     6121 gaccataaag catcgtttta ataccacgct tagccatcac ttcgataggc atacaacctt
     6181 cgaagtattt ttctttctca aaagaattta gtggtgcttc ctctgctgta gttaaagctt
     6241 cgtgaaatgc cataaattct tctttggtca ttggacaatt tagataagct gcttcacctt
     6301 tgtcatacct agacttaagg taaactttat tcatgtcaat cgtattttta tcgacaattg
     6361 gagcggcggc atcgtaaaaa taaaaaccgt caccaccatt aagttcatga attttagctg
     6421 ccagactatc acttgttaat ggtcctgtag cgataactgt aatagcatct ccagggatat
     6481 ctgtgatttc atcacgaatt acttcgatta atggatgttt atggatttcc tcagtaacag
     6541 cttcagaata accttctcta tcaacggcca tggctccacc tgctggaact cgatgtgcct
     6601 ccccatttct cataatgata gaatccaaac gacgcatttc ttcttttaaa agaccaactg
     6661 catttgttag agaatcaccg cggaatgaat tggaacatac taattctgca aaattatctg
     6721 ttttatgttg aggggttgat ttaaccccac gcatttcata aagcttaact ggtattccac
     6781 gtttagcaat ttgataagct gcttcagaac cagccaaacc agcgccgata acattaatgt
     6841 aagattgaga catgaactaa tacctcttga aagtagtcaa ctacttcctc aaatctttct
     6901 atataattta tttttccatt ataacataca tagctacttc ttcaaaaaac gttattcatt
     6961 aaactactag ttattctcta aataaaactc aaaacgatct ccaacatatt gcccatgaac
     7021 atattcaaaa ggcttaccat cagtaaagta tgaaacttgt gtcaatgcca aaatagcatg
     7081 gcctgcgtta acttctaaat gcgaagcaac acgttcacta gcatttctag catatatagt
     7141 ttgttggctt ttcccaattt cgtagccatt ctctgttaag gttttaaaga aatgttctgt
     7201 aacctctgat tgttcaaaac ctttaattaa attctctggt atagaggcta tctcataaac
     7261 caacggtacc ttatcagcat aacgtaccct ttccatacga accacataat ctgactgtga
     7321 taaatttaat ttttgaattt cggtttcatt tgctagtttt ctttgaaatg agattagttt
     7381 actggatggc ttacgccctt gagaattgac aatttctgta aaactagttg ttccccgcat
     7441 tttttcttga acacgatgac tagcaacata ggtgccacta ccaactcgtc tctccaaaat
     7501 accttcttca actaacaatg taattgattg gcgaagtgtc atacgactta cactataatc
     7561 atccgctaaa tctctttcgc taggtaaccg ctgcccaatc ttccaagtcc ctttatcgat
     7621 ttccttttta atagcgtcgt gaattttaat atatgctggt aacaatattc tctcctccta
     7681 gaatctacac ttcattttac catacttcta tatatttcta actctaaaat cgtgaaataa
     7741 ttcagaaaaa tcataagcaa tatttcttga taagaaagcg tttaatcaat tattttaatg
     7801 atgttaa
//
LOCUS       DATRTN010000077         7620 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.077, whole genome shotgun sequence.
ACCESSION   DATRTN010000077 DATRTN010000000
VERSION     DATRTN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.077"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..251)
                     /locus_tag="VBE22_001623"
     CDS             complement(<1..251)
                     /locus_tag="VBE22_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7203063.1"
                     /translation="MRKKSVFLRLWSYLTRYKATLFLAIFLKVLSSFMSVLEPFILGL
                     AITELTANLVDMAKGVSGAELNVPYIAGILIIYFFRGVF"
     gene            complement(256..1995)
                     /locus_tag="VBE22_001624"
     CDS             complement(256..1995)
                     /locus_tag="VBE22_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7203064.1"
                     /translation="MAYIWSYLKRYPNWLWLDLLGAMLFVTVILGMPTALAGMIDNGV
                     TKGDRTGVYLWTFIMFIFVVLGIIGRITMAYASSRLTTTMIRDMRNDMYAKLQEYSHH
                     EYEQIGVSSLVTRMTSDTFVLMQFAEMSLRLGLVTPMVMIFSVVMILITSPSLAWLVA
                     VAMPLLVGVVLYVAIKTKPLSERQQTMLDKINQYVRENLTGLRVVRAFARENFQSQKF
                     QVANQRYTDTSTGLFKLTGLTEPLFVQIIIAMIVAIVWFALDPLQRGAIKIGDLVAFI
                     EYSFHALFSFLLFANLFTMYPRMVVSSHRIREVMDMPISINPNAEGVTDTKLKGHLEF
                     DNVTFAYPGETESPVLHDISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGKIL
                     VDGVDVRDYNLKSLRQKIGFIPQKALLFTGTIGENLKYGKADATIDDLRQAVDISQAK
                     EFIESHQEAFETHLAEGGSNLSGGQKQRLSIARAVVKDPDLYIFDDSFSALDYKTDAT
                     LRARLKEVTGDSTVLIVAQRVGTIMDADQIIVLDEGEIVGRGTHAQLIENNAIYREIA
                     ESQLKNQNLSEGE"
     gene            complement(2062..2535)
                     /locus_tag="VBE22_001625"
     CDS             complement(2062..2535)
                     /locus_tag="VBE22_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7203065.1"
                     /translation="MIIRPIIKNDDQAVAQLIRQSLRAYDLDKPDTAYSDPHLDHLTS
                     YYEKIEKSGFFVIEERDEIIGCGGFGPLKNLIAEMQKVYIAERFRGKGLATDLVKMIE
                     VEARKIGYRQLYLETASTLSRATAVYKHMGYCALSQPIANDQGHTAMDIWMIKDL"
     gene            complement(2532..4400)
                     /locus_tag="VBE22_001626"
     CDS             complement(2532..4400)
                     /locus_tag="VBE22_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO7203066.1"
                     /translation="MSDFLVDGLTKSVGDKTVFSNVSFIIHSLDRIGIIGVNGTGKTT
                     LLDVISGELGFDGDRSPFSSANDYKIAYLKQEPDFDDSQTILDTVLSSDLREMALIKE
                     YELLLNHYEESKQSRLEKVMAEMDSLDAWSIESEVKTVLSKLGITDLQLSVGELSGGL
                     RRRVQLAQVLLNDADLLLLDEPTNHLDIDTIAWLTNFLKNSKKTVLFITHDRYFLDNV
                     ATRIFELDKAQITEYQGNYQDYVRLRAEQDERDAASLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFQNLKNDLHQTSDTSDLEMTFETSRIGKKVINFENVSFSYPDKSILKD
                     FNLLIQNKDRIGIVGDNGVGKSTLLNLIVQDLQPDSGNVSIGETIRVGYFSQQLHNMD
                     GSKRVINYLQEVADEVKTSVGTTSVTELLEQFLFPRSTHGTQIAKLSGGEKKRLYLLK
                     ILIEKPNVLLLDEPTNDLDIATLTVLENFLQGFGGPVITVSHDRYFLDKVANKIIAFE
                     DNDIREFFGNYTDYLDEKAFNEQNNEVISKKESTKTSREKQSRKRMSYFEKQEWATIE
                     DDIMILENTITRIENDMQTCGSDFTRLSDLQKELDAKNEALLEKYDRYEYLSELDT"
     gene            complement(4411..5619)
                     /locus_tag="VBE22_001627"
     CDS             complement(4411..5619)
                     /locus_tag="VBE22_001627"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEO7203067.1"
                     /translation="MRLNYLPSEFQKALPILKKIKKAGYEAYFVGGSVRDVLLDRPIH
                     DVDIATSSYPEETKQIFKRTVDVGIEHGTVLVLEKGGEYEITTFRTEEVYVDYRRPSQ
                     VNFVRSLEEDLKRRDFTVNAFALNEDGEVIDLFHGLDDLDNHLLRAVGLASERFNEDA
                     LRIMRGLRFSASLNFDIETTTFEAMKKHASLLEKISVERSFIEFDKLLLAPYWRKGML
                     ALIDSHAFNYLPCLKNRELQLSAFLSQLDKDFLFETSEQAWASLILSMEVEHTKTFLK
                     KWKTSTHFQKDVEHIVDVYRIREQMGLAKEHLYRYGKTIIKQAEGIRKARGLMVDFEK
                     IEQLDSELAIHDRHEIVVNGGTLIKKLGIKPGPQMGDIISQIELAIVLGQLINEEEAI
                     LHFVKQYLMD"
     gene            complement(5804..6652)
                     /locus_tag="VBE22_001628"
     CDS             complement(5804..6652)
                     /locus_tag="VBE22_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO7203068.1"
                     /translation="MKLALITDTSAYLPEAIENHEDVYVLDIPIIIDGKTYIEGQNLT
                     LDQYYDKLAASKELPKTSQPSLAELDDLLCQLEKEGYTHVLGLFIAAGISGFWQNIQF
                     LIEEHPNLTIAFPDTKITSAPQGNLVRNALMCSREGMDFDVIVNKIQSQIEKIEGFIV
                     VNDLNHLVKGGRLSNGSAIIGNLLSIKPVLHFNEEGKIVVYEKVRTEKKALKRLAEIV
                     KEMTADGEYDIAIIHSRAQDKAEQLYNLLAKAGLKDDLEIVSFGGVIATHLGEGAVAF
                     GITPKN"
     gene            complement(6652..7032)
                     /locus_tag="VBE22_001629"
     CDS             complement(6652..7032)
                     /locus_tag="VBE22_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEO7203069.1"
                     /translation="MEVIREQEFVNQYHYDARNLEWEEENGTPKTNFEVTFQLANRDE
                     AAKVTSIVAVLQFVIVRDEFVISGVISQMAHIQGRLINEPSEFSQDEVENLAAPLLEI
                     VKRLTYEVTEIALDRPGVTLEFNS"
     gene            complement(7104..7613)
                     /locus_tag="VBE22_001630"
     CDS             complement(7104..7613)
                     /locus_tag="VBE22_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014634934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203070.1"
                     /translation="MYKNYPNIPYISPERDLAEINFSKVVPRKNMEETSEGLLPGDII
                     LLWRIQFGTFTTETSFSKYFEYIYGINGKEHLEFLIKNGFVRMESPLDSLDHLSAPLL
                     KLFLKEKNVKGLSKMKRSDLDQAIAIAFTEEELGKLFVVRGLALTEKGLAALSNNQEV
                     IDRHPKKKF"
BASE COUNT         2451 a         1572 c         1103 g         2494 t
ORIGIN      
        1 tagaaaacac ctctgaaaaa ataaataatc aaaataccag caatataagg aacgttcaat
       61 tctgccccag aaactccctt agccatatca acaaggttag cagtcaactc tgttatcgct
      121 aaccctaaaa taaaaggctc cagaacactc ataaaactag ataaaacttt caaaaaaatc
      181 gctaagaaaa gagtagcttt gtagcgagtt aggtaagacc ataatctcaa aaaaacagat
      241 ttttttctca tacaatcact ctccttctga taagttttgg ttcttcagtt gtgactcagc
      301 gatttcacga taaatagcat tattttctat taattgagcg tgggtaccac gaccgacaat
      361 ttcgccttca tcaaggacaa taatctgatc agcatccata atcgtaccca ccctttgagc
      421 aactatcaaa actgtagaat caccggttac ttcttttaga cgcgctctta aagtagcgtc
      481 tgtcttataa tcgagagcag aaaatgaatc atcaaaaata tataaatctg gatctttaac
      541 aacagcccta gcaatagata accgttgttt ttgaccccca gaaagattgc tcccaccttc
      601 agctaaatgc gtttcaaagg cttcttggtg actctcaata aactctttag cttgagaaat
      661 atcaaccgct tgtctaagat catcaatagt agcatcagct tttccatatt ttaaattctc
      721 tcctattgtc cctgtaaata aaagagcttt ttgggggata aatccaatct tttggcgaag
      781 tgatttaagg ttataatctc ttacatcaac tccatctact aagatttttc caagtgtcac
      841 atcataaaaa cgtggaatca aattaacaag agaagatttt cctgaacctg ttgaaccaat
      901 aaaagcaatt gtttctccag gcttagcttt aaaagaaata tcatgcaaaa cgggactctc
      961 tgtttctcct ggataagcga atgttacatt atcaaattct aaatgccctt taagtttcgt
     1021 atccgtaaca ccttcggcat taggattgat agagattggc atatccatca cctctctaat
     1081 acgatggctt gataccacca tacgaggata catagtaaaa agattggcaa atagcaaaaa
     1141 tgaaaagaga gcatggaagc tatattcgat aaaagcaact aaatccccta ttttaatagc
     1201 acctctttgt aagggatcca aagcaaacca aacgatagcc acaatcattg caataataat
     1261 ttgaacgaaa agtggttctg ttagccctgt taatttaaaa agaccagttg aagtatctgt
     1321 gtaacgttgg ttagcgactt gaaatttttg tgattgaaaa ttctctcttg caaaggctct
     1381 aacaacgcgt aaccctgtta aattttcacg aacatattga ttgattttat caagcatagt
     1441 ctgttgtctt tcagataaag gttttgtttt tatagctaca tataaaacga ctcctaccaa
     1501 aagaggcatc gcaaccgcta caagccaagc caaagatgga ctcgtaatta gtatcataac
     1561 cacgctaaaa atcattacca taggagttac taggcctaaa cgtaaagaca tttcagcaaa
     1621 ttgcatcaaa acaaaagtat cgcttgtcat acgtgtcact agtgaagata cacctatctg
     1681 ttcatattca tgatgggagt attcttgaag cttagcatac atatcattac gcatatctct
     1741 aatcattgtt gtcgttaagc gactagatgc gtaagccatc gtaatacgcc caataatacc
     1801 tagtacaaca aatataaaca tgatgaacgt ccacagataa actccagtcc gatcaccttt
     1861 tgtaacgcca ttatcaatca tacccgctaa ggctgtgggc attcctagga taaccgtcac
     1921 aaaaagcata gctcctagta aatcaagcca taaccaattg gggtaccttt tcaaataaga
     1981 ccaaatatat gccatatatg tctccttttc tttcctctta tctcaaaata attaatccat
     2041 gttcactaat ccactttcaa cttataaatc tttaatcatc caaatatcca tagctgtatg
     2101 accttgatca tttgctattg gttgcgataa ggcacaatat cccatatgct tataaaccgc
     2161 agttgcccta ctcaaagtac tggctgtctc taaataaagt tgtctatacc caatttttcg
     2221 agcttctact tcaatcattt tcactaaatc agtagcaagc cccttaccac ggaaacgttc
     2281 tgcaatgtac accttctgca tctctgcaat tagatttttc agcggaccaa agccgccaca
     2341 gccaataatc tcatctctct cctcaatgac aaagaatcct gacttctcta ttttttcgta
     2401 gtatgaggtc aaatgatcta agtgagggtc tgaatatgct gtatcaggtt tatctaaatc
     2461 ataggcgcgt aaactttgtc gaattaattg tgcaactgct tggtcatcat ttttaataat
     2521 cggacggata atcatgtgtc taactcacta aggtactcat aacggtcata cttttctaga
     2581 agtgcttcat tttttgcatc taattccttt tgtaaatcag ataaccttgt aaaatcacta
     2641 ccacatgttt gcatatcatt ttctatacga gtgatagtat tttccaatat cataatatcg
     2701 tcttcaattg tcgcccattc ttgtttttca aagtaagaca ttcttttacg actttgcttt
     2761 tcacgacttg tcttggtact ctctttttta ctgataactt cattattttg ctcattaaat
     2821 gctttttcat ctaaataatc agtataatta ccaaaaaatt cacggatatc gttatcttca
     2881 aacgcaataa ttttattagc cactttatct aaaaagtaac gatcgtgact aactgtaatc
     2941 acaggaccac caaagccttg taaaaaattt tcaagaacag ttaatgtagc aatatctaag
     3001 tcatttgtcg gctcatcaag tagtaacaca ttaggctttt caatcaggat ttttaaaagg
     3061 taaagtcttt ttttctcacc acctgataat tttgcaattt gtgttccatg tgtcgaacgt
     3121 ggaaagagaa attgttccaa tagttctgtc acacttgttg taccgacact agttttaacc
     3181 tcatctgcaa cctcttgcaa ataattaata acacgttttg agccatccat attatgaagt
     3241 tgttgtgaaa agtaacctac acgtatcgtt tcaccaatag agacattacc cgaatccggc
     3301 tgtaaatctt gaacaattaa attaagtaag gttgactttc caacaccatt atctccaacg
     3361 atgccaatac ggtctttatt ttgaattaac aaattaaagt ctttcaagat agatttatct
     3421 gggtaagaaa aagagacatt ttcaaaatta ataacctttt tcccaattcg acttgtttca
     3481 aatgtcattt ccaaatcgct tgtatcgctt gtttggtgta aatcgttttt tagattttga
     3541 aaacgattaa tacgagcctg ttgtttcgtt gcacgagctt gtggctgagt acgcatccaa
     3601 gctagttcct gtttataaag ctgtttcttt ttatgtaaac tagcagcatc acgctcgtct
     3661 tgttctgcac gaagtcggac ataatcctga taattgcctt gatattctgt aatctgtgcc
     3721 ttatctaatt caaaaatacg tgttgcaaca ttgtctagaa aataacgatc atgagttata
     3781 aaaagcactg tctttttact atttttcaaa aaattcgtta accatgcaat agtgtcaata
     3841 tctaagtggt tagtaggttc gtctaagagc aataaatctg catcatttaa taatacttgc
     3901 gctaattgaa cacgtcttcg taatcctcct gataattcac caaccgacaa ctgcaaatca
     3961 gtaataccta atttggataa tactgttttg acttcgctct caatagacca agcatctaaa
     4021 gaatccattt ctgccattac tttctctaga cgtgattgct tactttcttc gtagtgatta
     4081 agcaataatt catattcttt aattaaagcc atctctctta agtcagaaga aagtacggtg
     4141 tccaaaattg tctgagaatc atcaaagtct ggttcttgtt ttaaataagc aatcttataa
     4201 tcattagctg atgaaaaagg ggaacgatca ccatcaaaac ctaattcacc cgaaataaca
     4261 tctaatagtg ttgtctttcc agttccattg acaccaataa tcccaatacg gtctaaacta
     4321 tggatgataa atgaaacatt actaaagacc gtcttatcac caaccgactt agtcaatcca
     4381 tctactaaaa aatcgctcat ataatcctct ctaatccatc aagtactgct taacaaaatg
     4441 taaaatagcc tcttcttcat taatcagttg tcctaaaaca atggctaatt caatttgaga
     4501 gataatatct cccatctgtg gaccaggttt tattcctaat ttcttgatta aggtgccacc
     4561 attgacaact atctcatgcc tatcatggat tgctaactca ctatctagtt gttctatttt
     4621 ttcgaaatca accatcaagc ctcttgcttt gcgaatacct tccgcttgtt ttattatagt
     4681 ttttccataa cgataaagat gttctttagc caatcccatt tgttcacgaa tacgataaac
     4741 atccactata tgctcaacat ctttttgaaa gtgagttgat gtcttccatt tttttaaaaa
     4801 agtctttgtg tgttcaactt ccatactcaa gatgagtgat gcccaagctt gttctgatgt
     4861 ctcaaataaa aaatctttat ctaactggga caaaaaggcg cttaattgta attccctatt
     4921 ttttaagcat ggtagataat taaaagcatg actatctatt agtgccagca ttcctttacg
     4981 ccaataaggt gctaatagaa gtttatcaaa ttcaataaaa gaacgctcaa cagatatttt
     5041 ctccaataat gacgcgtgct ttttcattgc ctcaaaagtt gttgtttcga tatcaaaatt
     5101 tagactagca gaaaaacgca aacctcgcat aattcgaaga gcatcttcat taaaacgctc
     5161 actggcgagt cctactgctc taagaaggtg attatctaaa tcatccaatc catgaaagag
     5221 atcaataact tctccatctt catttaaagc aaaggcatta actgtgaaat ctcgacgttt
     5281 tagatcttct tctagcgacc taacaaaatt gacctgactc ggacgcctat aatcaacata
     5341 aacctcctca gtcctaaaag tggtaatctc atattcacca cctttttcca atactaagac
     5401 tgttccatgt tcaataccaa catcaactgt ccttttaaaa atctgctttg tttcttctgg
     5461 ataagatgac gttgctatat caacatcatg aattggacgg tcaagtaaaa catcccgaac
     5521 actaccacct acaaagtatg cttcataacc ggcttttttt atctttttta atattggtaa
     5581 agccttctga aactcagaag gcaaataatt taatctcatc atactattcc aaatctaaac
     5641 gaactaaagt cgcattatta tttcttataa atggattcag aaacaaaaag aactgcctaa
     5701 gggacactcc ctacctactc aaattatcaa aaaagaatct cctaacttct tctttgatat
     5761 ctgcaataag gatcaacctc caagcaactc tttttatcac acatcaattt ttgggggtaa
     5821 taccaaaggc aaccgcacct tcaccaagat gagttgcaat aacgccacca aatgatacaa
     5881 tttctaaatc gtctttaaga ccagctttag ctaacaaatt atataattgt tctgccttat
     5941 cttgtgctct agagtgaata atagcaatat catattcacc atctgctgtc atttctttga
     6001 caatctcagc caagcgtttt aaggcttttt tctctgtacg cactttctca tagacaacga
     6061 ttttaccttc ttcattaaaa tgtaagaccg gcttgatact taaaaggtta ccaatgatag
     6121 ctgaaccgtt tgacaatcta ccacctttta ctagatgatt caaatcatta acaacaataa
     6181 atccttcaat tttttcaatt tgtgattgta tcttgttaac tatgacatca aaatccatcc
     6241 cttcacgtga acacatcaaa gcatttctaa ctaaattgcc ctgtggtgct gaagttattt
     6301 tagtatccgg aaaagcaatt gtcaaatttg gatgttcttc aataagaaac tgaatattct
     6361 gccagaaacc tgatatcccc gctgcaatga ataaaccaag gacgtgtgta tacccttctt
     6421 tctccaattg acataagaga tcatctagtt cagctaagct aggctgactt gttttaggta
     6481 attcttttga tgccgctagc ttatcataat attggtctaa tgtcagattt tgtccctcaa
     6541 tataagtttt accatcaatg ataattggaa tatctaaaac atagacatct tcatgatttt
     6601 cgatagcctc aggcaagtaa gctgaggtat ccgtaataag tgctaatttc attaactatt
     6661 aaactccaat gttactcctg gtctatctaa agcaatttct gtaacttcat aagtcagacg
     6721 tttaacgatt tctaaaagag gagctgctag attctcaact tcatcttgac taaactcact
     6781 aggctcattg ataaggcgcc cttggatatg agccatttgt gaaatgacac cagaaataac
     6841 aaactcatca cgtacaataa caaattgtaa cactgctaca atgctcgtta cttttgctgc
     6901 ttcatctcta ttagctaatt ggaaagtaac ttcaaaattt gtttttgggg taccattttc
     6961 ctcttcccat tctaaattgc gcgcatcata gtggtattga ttgacaaatt cttgttcacg
     7021 aatgacttcc atgtattttc tccttgatag acaatatgta ttattatagc ataaatttgt
     7081 agattcttaa agcaggtggg aatctagaac ttcttcttag gatgtctatc aatcacttct
     7141 tgattgttac tcaaggctgc taaacctttt tctgtaagtg ccaaccctct gacaacaaac
     7201 aacttcccta attcctcttc tgtaaaagca atagctattg cctgatctaa atcagagcgt
     7261 ttcatctttg acaagccttt tacgtttttt tcttttaaga atagttttaa aagtggagca
     7321 gataagtgat ctaaagaatc aagcggtgac tccattctta caaatccatt ttttattaaa
     7381 aattctaggt gttcttttcc atttatacca taaatgtatt caaaatattt tgaaaaactt
     7441 gtttcagttg tgaaagttcc aaactgtata cgccataata agataatatc tccaggcaag
     7501 agtccttcac tagtttcttc catatttttc ctaggaacaa ctttactaaa attaatttca
     7561 gccaagtctc tttcaggtga gatatagggg atattaggat aatttttgta aatttcttca
//
LOCUS       DATRTN010000078         7487 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.078, whole genome shotgun sequence.
ACCESSION   DATRTN010000078 DATRTN010000000
VERSION     DATRTN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.078"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..198)
                     /locus_tag="VBE22_001631"
     CDS             complement(<1..198)
                     /locus_tag="VBE22_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203071.1"
                     /translation="MKDYINRILHFIKEHMTYHVNFIDDFLDIKWEKVSNIHLRFWTT
                     IIAYLVIFILSISTVILNLVLL"
     gene            complement(347..1087)
                     /locus_tag="VBE22_001632"
     CDS             complement(347..1087)
                     /locus_tag="VBE22_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7203072.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENI
                     TFAPIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPD
                     ILLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRF
                     LEDGTPEQIFDTPQHPRLQDFLNKVLNV"
     gene            complement(1087..3270)
                     /locus_tag="VBE22_001633"
     CDS             complement(1087..3270)
                     /locus_tag="VBE22_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO7203073.1"
                     /translation="MKHKLKAFTLALLSIFFVFGGKVSAETVNIVSDTAYAPFEFKDS
                     DQTYKGIDVDIVNEVAKRAGWNVNMTYPGFDAAVNAVQSGQADALMAGTTVTEARKKV
                     FNFSDTYYDTSVILYTKNNNKVTNYKQLKGKVVGVKNGTAAQSFLEENKSKYGYKVKT
                     FDTSDLMNNSLDSGSIYAAMDDQPVVQFAINQGKAYAINMEGEAVGSFAFAVKKGSGH
                     DNLIKEFNTAFAQMKSDGTYNDIMDKWLGKDATKTSGKATGNANEKATPVKPSYKIVS
                     DSSFAPFEYQNGKGKYTGFDMELIKKIAKQQGFKLDISNPGFDAALNAVQSGQADGVI
                     AGATITEARQKIFDFSDPYYTSSVILAVKKGSNVKSYQDLKGKTVGAKNGTASYTWLS
                     DHADKYNYHVKAFDEASTMYDSMNSGSIDALMDDEAVLAYAINQGRKFETPIKGEKSG
                     DIGFAVKKGANPELIKMFNNGLASLKKSGEYDKLVKKYLSTASTSSNDKAAKPVDEST
                     ILGLISNNYKQLLSGIGTTLSLTLISFAIAMVIGIIFGMMSVSPSNTLRTISMIFVDI
                     VRGIPLMIVAAFIFWGIPNLIESITGHQSPINDFVAATIALSLNGGAYIAEIVRGGIE
                     AVPSGQMEASRSLGISYGKTMQKVILPQAVRLMLPNFINQFVISLKDTTIVSAIGLVE
                     LFQTGKIIIARNYQSFRMYAILAIMYLVIITLLTRLAKRLEKRLK"
     gene            3453..4373
                     /locus_tag="VBE22_001634"
     CDS             3453..4373
                     /locus_tag="VBE22_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7203074.1"
                     /translation="MNKRILNSQFAVYKNWPSWLLLLFACFMVYLFFFVGLTLGGVGI
                     VLVSSVLYKFSSYTFFEFINLFNNIYGELGTFSFSAFLLLIWVKFVEKRPFSELGFST
                     KFKRILWSLIKGWSIGFILFSISVITAYILGGLDFHSYDVSKATIFYVVTLLPFWLIQ
                     SGTEELLTRGWLLPLINHRFHLAVAIGVSSTLFGILHLVNAHVTFLSIVSIICSGVLM
                     SLYMIKSGNIWSVAALHGAWNFSQGNLYGIAVSGQKAGASLLHFTVKENAPDWISGGA
                     FGIEGSLISIFVLLAAIIYLLWLIKTEETD"
     gene            4434..6425
                     /gene="uvrB"
                     /locus_tag="VBE22_001635"
     CDS             4434..6425
                     /gene="uvrB"
                     /locus_tag="VBE22_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEO7203075.1"
                     /translation="MIDRKDTNRFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILKG
                     ATGTGKTYTMSQVIAQVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGQEISRDQLLNNLVDIQFERNDIDFQRGKFRVRGDVVEVFPASRDEHAFR
                     IEFFGDEIDRIREIESLTGRVLGEVEHLAIFPATHFMTNDEHMEEAISKIQAEMENQV
                     ELFEKEGKLIEAQRIRQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPFTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPSMGQMDDLLGEINLRTEKG
                     ERTFITTLTKRMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSNGHVIMYADKITDSMQ
                     RAMDETARRRRLQMDYNEKHGIVPQTIKKEIRDLIAITKSNDSDKPEKVVDYSSLSKK
                     ERQAEIKALQQQMQEAAELLDFELAAQIRDVILELKAID"
     gene            complement(6522..>7487)
                     /locus_tag="VBE22_001636"
     CDS             complement(6522..>7487)
                     /locus_tag="VBE22_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO7203076.1"
                     /translation="FLFESGTNIRPTTHLSDIFVFFDTLFILTWKRHHHKVKIPNSKL
                     FSSLKKIFIYEELNTCVKSIVQPYFNVIFNSEDMDYIFLIYLTSANSFAAQKWTETHV
                     DEVLAIFKSLPKFQMLLQPLKEALPYSDTYYDELVKVTIYFSRNFIVGLNQLIPETLS
                     FPSTFYPGNNPKLISIIEPIVTHWLTEIGESKFKKTHFFLLCAHLERVIKNSMPPINI
                     VLLTTDFINNEILKDYILQSLSTDKIRFHSYYLLTDNISQISTLKPDLIITNRKLFTY
                     VTKELATNSLVAHIDYNDTPSQISNIQTIISNIKEEKYRKIFDKI"
BASE COUNT         2373 a         1194 c         1415 g         2505 t
ORIGIN      
        1 aagaagtaca aggttaagaa ttacggtaga gatacttaag ataaaaataa ctaaataagc
       61 aataatagtt gtccaaaacc ttaaatggat gttactcacc ttttcccatt taatatctaa
      121 aaagtcatct ataaagttga catgataagt catatgttct ttaataaagt gcaatattct
      181 gttaatatag tctttcatac tgctatatta tcatactgaa agcccttgtg caacattatt
      241 caaacattct agctgatgtt ttgctaattt tagaagaaaa cttatcctat tataatttgt
      301 ttgtgattag gatataaaaa aagatgacac gaagtcatct tttttattaa acgtttaaaa
      361 ctttattaag gaagtcttgg agacgtgggt gttgaggtgt atcaaagatt tgctcaggtg
      421 tcccatcttc gaggaaacga ccaccatctg taaagataac acgattggca acttgacgag
      481 caaatcccat ttcgtgtgtc acaattaaca tagtcatacc ttgttcagct aaatctttca
      541 taacattgag aacatctcca accatttctg gatcaagtgc agaagttggt tcgtcaaaaa
      601 gtaagatatc tggattcatc gctaagcttc ttgcgatagc tacacgttgt ttttgtcctc
      661 cagataaact atctggtttg gcattagctt tatcggctaa accaactttt tcaagtaatt
      721 ccatcccatg cttttcagct gcttctttag attctttacc aagttctata ggagcaaaag
      781 tgatgttttc aagaactgac atatgtggga aaagattgaa gtgttgaaat accattccga
      841 tattttcacg tgctttgtct atgtctgttt ttgggtttga taactcaaag ccgtccacaa
      901 caacttttcc acttgtgatt gattcaagta agttcaatgt gcgaaggaaa gttgattttc
      961 ctgaaccaga aggtccaata atacaaacaa catcaccttc ataaaattta gcatcaatac
     1021 cttttaaaac ttcattttgc ccatacgact tgtgtaaatc ttggacatca atttttaatt
     1081 ctgccattat ttaagcctct tttctaaacg ttttgctaga cgggtgagaa gggtaatgat
     1141 tacaagatac ataattgcaa ggatagcata catacggaat gattgatagt tacgtgcaat
     1201 tatgatttta ccagtttgga agagttccac aagtccgatt gctgatacaa ttgttgtatc
     1261 ctttaatgag atgacaaatt ggttgataaa gtttggtaac ataaggcgta ctgcttgagg
     1321 caagataacc ttttgcatag ttttgccgta agaaatacct aaactacgac ttgcttccat
     1381 ttgaccagaa ggaacagctt caataccacc acgtacaatt tcagcaatgt acgcaccacc
     1441 atttaaagaa agagcgatag tagcagcaac gaagtcatta attggacttt ggtgacctgt
     1501 gatgctttcg attaaattag gaatacccca gaaaataaaa gcggccacaa tcatgagtgg
     1561 aataccacgg acaatatcaa caaaaatcat tgaaattgtg cggagagtat tacttggtga
     1621 tacgctcatc ataccaaaga taataccaat aaccatagca atcgcaaatg agataagagt
     1681 taaacttaaa gtagttccaa taccagatag caattgtttg tagttattag aaattaaccc
     1741 taaaatagtt gattcatcta caggtttagc agctttatcg tttgaagagg tgctggctgt
     1801 ggaaaggtat tttttaacaa gtttatcgta ctcacccgat tttttgagtg aagcaagacc
     1861 gttgttaaac attttaatta attctggatt tgcccctttt ttcactgcaa atccgatatc
     1921 gcctgatttt tcacctttga taggtgtttc aaatttacga ccttgattaa tagcgtaagc
     1981 aagaactgct tcgtcatcca ttagagcatc aattgaacct gagttcatac tatcatacat
     2041 tgtagatgct tcatcaaatg ctttaacatg atagttgtac ttatctgcgt ggtctgataa
     2101 ccaagtatat gaggcagtac catttttagc accaactgtt tttcctttta aatcttggta
     2161 tgatttgaca ttgcttcctt ttttaaccgc taagataacg ctagatgtgt aataaggatc
     2221 agaaaaatca aagatttttt ggcgtgcttc tgtgattgta gctcctgcaa taacaccgtc
     2281 agcttgtcca gattggacag catttaaagc ggcatcaaaa cctggatttg agatatcaag
     2341 tttaaaacct tgctgtttag caattttctt gattaattcc atatcaaaac cagtatattt
     2401 ccctttaccg ttttgatatt cgaatggtgc gaatgaagaa tcagaaacaa ttttataact
     2461 tggctttaca ggagttgctt tttcattggc attacctgtt gctttgccgc ttgtttttgt
     2521 agcgtctttc ccaagccatt tatccatgat gtcattataa gtgccatctg atttcatttg
     2581 tgcaaaagct gtgttaaatt ctttaattag gttatcgtgt ccactacctt ttttgacagc
     2641 aaatgcaaag ctaccaactg cctcgccttc catgttaatg gcgtaagctt ttccttgatt
     2701 tatcgcaaat tgcacaactg gttgatcgtc catagcggcg taaatagaac cagaatcaag
     2761 gctgttattc attaggtcac ttgtatcaaa tgttttaact ttatagccgt atttagattt
     2821 attttcctct aagaagcttt gagcagctgt tccatttttt acaccgacta cttttccttt
     2881 tagttgtttg tagtttgtga ctttattatt atttttagta taaagaataa cggaagtatc
     2941 gtaataagta tctgagaaat taaagacttt tttacgtgct tcagtaacag tagttccggc
     3001 cattagcgcg tctgcctgtc cagattgaac agcgttaact gcggcatcaa aacctgggta
     3061 cgtcatgtta acattccagc cagcacgctt agcgacttcg ttaacgatgt caacatcgat
     3121 tcctttataa gtttgatcag aatctttaaa ttcgaatgga gcgtatgctg tatcagaaac
     3181 aatattcaca gtctctgcac tgacctttcc accaaacaca aagaatattg agagtaaagc
     3241 aagcgtaaaa gcttttaact tgtgcttcat gtaaatctcc tttttataaa tatgtgtttt
     3301 attatatcaa aataaataag atagagcaac aaaatgtcat ttctatgtta gaaaacgtaa
     3361 catgtatgtt atgttaattt tatatatatc tttagttagt attatgatat aatcgttcta
     3421 gtgttatcac acaaaaagga tttaggatta ttatgaataa aaggatttta aatagtcagt
     3481 tcgctgtcta taaaaactgg ccgtcatggc tattattgtt atttgcttgt tttatggtat
     3541 acctattttt tttcgttggt ctaactctag gtggtgtggg aattgttcta gtgtcttctg
     3601 tactctataa gttttcttcc tatacttttt ttgagtttat taatttattt aataatatct
     3661 atggagaatt aggaaccttt agtttttcag cattcctttt gcttatctgg gtaaaattcg
     3721 tagaaaaaag acctttttca gaattggggt tttctacaaa gtttaaaaga attctgtggt
     3781 cacttataaa aggctggagt ataggtttta ttctattttc aatttctgtc atcactgctt
     3841 atatattggg aggacttgac tttcatagct atgatgtttc taaggcaacg attttctatg
     3901 ttgtaactct ccttcctttt tggcttattc aaagcggtac ggaagaattg ctaacacgag
     3961 ggtggcttct tcctctaatt aatcatcgtt ttcatttagc tgttgccata ggggtctcat
     4021 ccactttatt tggcattttg cacttggtaa atgcccatgt cacttttctt tctattgtaa
     4081 gtattatatg ttcgggagtt ttaatgtcct tgtatatgat taaaagtggt aatatttgga
     4141 gtgttgcagc cttacatgga gcttggaatt ttagccaagg taatctctat ggtattgccg
     4201 taagtggtca gaaagcagga gcatctcttt tgcattttac cgttaaagaa aacgcaccgg
     4261 actggatatc aggtggtgca ttcggaatag aaggaagttt gatttctatt tttgttctct
     4321 tagcagcaat tatatacctt ctatggctaa ttaagactga agaaacagat tgagtgttgc
     4381 agctcagtct ttttcttttt tctatgttaa aataaagtaa tcgaggtaaa gaaatgatag
     4441 atagaaaaga tactaaccga tttaaattag tttccaaata tagcccttcg ggagatcaac
     4501 ctcaagctat tgaaacgttg gttgacaata ttgaaggagg agaaaaagct cagattttaa
     4561 aaggtgctac tggtactggt aaaacctaca caatgagcca agttatcgcc caagttaaca
     4621 agccaacctt ggtgattgca cataataaaa ccttagcagg tcaactttat ggagagttta
     4681 aagaattttt tcctgacaat gccgtagagt actttgtttc atattatgat tattatcaac
     4741 cagaagctta tgtgccttct tcggatactt atattgaaaa agatagttca gtcaatgacg
     4801 aaattgacaa attacgtcac tcagcgactt cttcgttatt agaaagaaat gacgttattg
     4861 ttgtagcatc tgtttcttgt atttacggtt taggttctcc taaagagtat gcagacagtg
     4921 ttgttagcct taggccaggt caagaaattt cacgtgatca gttattgaac aatctcgttg
     4981 atattcaatt tgagcgtaac gatattgatt ttcaacgtgg aaaatttcgc gttcgtggcg
     5041 atgttgtaga agtttttcct gcgagtcgtg atgagcatgc ctttcgaatt gaattttttg
     5101 gtgatgaaat tgatcgtata agagaaatag aatcgttaac agggcgtgtg ctaggagaag
     5161 tggaacattt agctattttc ccagcaacac actttatgac aaatgatgaa catatggaag
     5221 aagctatttc aaaaatacaa gctgaaatgg aaaatcaagt agaacttttt gaaaaagaag
     5281 ggaaattgat tgaagcgcaa cgtattcgac aaaggacaga atatgatatt gaaatgcttc
     5341 gtgaaatggg atatacaaat ggtgttgaga actactcacg tcacatggat ggtagaagtg
     5401 aaggtgagcc accttttact ttgttagatt tcttcccaga agattttttg attatgattg
     5461 atgagagtca catgacaatg gggcaaatca aagggatgta taatggagac cgttcgcgta
     5521 aagaaatgtt ggttaattac ggtttccgtt taccatctgc cttagataac cgtccacttc
     5581 gtcgtgaaga attcgaaagc catgtccatc aaattgtcta tgtatctgca acgccaggag
     5641 actatgaaat ggagcaaaca gatacagttg ttgagcaaat tatccggcca acaggacttc
     5701 ttgatccaga agttgaagtt agaccaagta tggggcagat ggatgacttg cttggtgaaa
     5761 tcaatttgag gactgaaaaa ggtgagcgta cctttattac aacattgaca aagcgtatgg
     5821 cagaagactt aacagattat ctcaaagaaa tgggagttaa agttaagtat atgcacagcg
     5881 atattaaaac cttagaacgt acagagatta ttcgtgattt acgacttggt gtgtttgatg
     5941 ttttaattgg tattaatctc ttacgtgaag gaatagatgt tcctgaggtt tcccttgttg
     6001 caatcttaga tgctgataag gaaggtttcc ttcgtaatga gcgtggtctt atccaaacaa
     6061 tcggacgtgc agcacgtaac agtaatggtc atgttattat gtatgctgat aaaataacag
     6121 attctatgca aagagctatg gatgaaaccg ctcgccgtcg tcgtttacaa atggattata
     6181 atgaaaagca tggaattgtg ccacaaacaa ttaaaaaaga aatccgtgat ttaattgcta
     6241 ttactaaaag taatgacagc gataagcctg aaaaagttgt cgattacagt agtttatcta
     6301 aaaaagaaag gcaagctgaa attaaagctc ttcaacagca aatgcaagaa gcagctgaat
     6361 tattggactt tgagctagct gcacaaatcc gagatgttat cttagaacta aaagcaatcg
     6421 attaatacga tgaacagaat taataaaacg actagagtag ttaatgaagt gctctagtcg
     6481 ttttattaat aattgaaatc aaaagaatac agtaggtgat gttatatttt atcaaagatt
     6541 tttcgatatt tttcttcttt aatattagaa ataatagttt ggatattact gatttgagag
     6601 ggtgtatcgt tgtaatcaat atgagcgact aaactatttg tagctagttc ctttgtaaca
     6661 taggtaaaca gttttcgatt agtgataatt aaatcgggtt ttaaggtcga tatttgacta
     6721 atattatctg tcaataagta ataggaatga aatctaattt tatctgttga aagggattgc
     6781 aaaatatagt cttttaaaat ttcattatta ataaaatcag ttgtcagcaa cacaatattt
     6841 ataggaggca tgctattctt tattactctt tcaaggtgag cacagagcaa gaagaagtgc
     6901 gtttttttga actttgattc tccaatttca gttagccaat gagtgactat tggttcgata
     6961 atggatatta gtttaggatt atttccagga tagaatgttg aaggaaaact aagtgtttca
     7021 ggaattaatt gattcagacc aactatgaag tttcgagaaa agtaaatagt gactttcact
     7081 agctcatcgt agtaggtgtc agagtatgga agagcttctt tcaaaggttg tagtagcatt
     7141 tggaatttag gaagactttt aaaaatagct aacacttcat cgacatgtgt ttcagtccac
     7201 ttttgagcag caaaagagtt agcgcttgta agatagataa gaaaaatata atccatgtct
     7261 tctgaattaa aaataacatt gaagtaaggt tgaacgatac tttttacaca tgtattcagc
     7321 tcctcataaa taaagatttt tttaagagaa gagaaaagtt tggagtttgg aattttaact
     7381 ttatgatggt gacgtttcca ggttaaaatg aaaagcgtat caaaaaaaac aaagatatca
     7441 gaaaggtgcg tagtaggtct tatattagta ccactctcaa ataaaaa
//
LOCUS       DATRTN010000079         7374 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.079, whole genome shotgun sequence.
ACCESSION   DATRTN010000079 DATRTN010000000
VERSION     DATRTN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.079"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(172..633)
                     /locus_tag="VBE22_001637"
     CDS             complement(172..633)
                     /locus_tag="VBE22_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203077.1"
                     /translation="MAKIGLNFGEKLQIADKEYRTFNDGLDVNVLLGDLTWRRYEEPV
                     QFTEDDTTQPPDRFGRYPQKPTGEVKWNDIAVFSTAQNETIFVSITDMPSYAIEDLGL
                     KLGDPIELSDVVVTWSNVSGGMFKIFASSLKKKDVANNGNASKNEASKVNK"
     gene            complement(655..951)
                     /locus_tag="VBE22_001638"
     CDS             complement(655..951)
                     /locus_tag="VBE22_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203078.1"
                     /translation="MKQETLVLLPNFIGDAIYEGFEAGPAQLNGKKGSYTRHILFNEK
                     HGKFYVYTPASSRVIGNGKMVTVEKPLLFIDNTINGRNIEPAKNVWAEKLIEVK"
     gene            complement(1011..3602)
                     /locus_tag="VBE22_001639"
     CDS             complement(1011..3602)
                     /locus_tag="VBE22_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7203079.1"
                     /translation="MYKNQNTKGHGFFRKSKAYGLVCGIALAGAFTLATSQVSADQVT
                     TQATTQTVTQNQAETVTSTQLDKAVATAKKAAVAVTTTAAVNHATTTDAQADLANQTQ
                     TVKDVTAKAQANTQAIKDATAENAKIDAENKAESQRVSQLNAQTKAKIDAENKDAQAK
                     ADATNAQLQKDYQAKLAKIKSVEAYNAGVRQRNKDAQAKADATNAQLQKDYQAKLALY
                     NQALKAKAEADKQSINNVAFDIKAQAKGVDNAEYGNSIMTAKTKPDGSFEFNHDMIDG
                     VKTIGYGKLTGKVNHHYVANKDGSVTAFVDSITLYKYEYRNVAQNAAVNQNIAFRVLT
                     KDGQIIFEKKHNGNKTFAETLNKTLQLNLKYELKPHGSSGSVEVFKIHDDWVHDTHGS
                     ALVSYVNNNDVVPNVVIPKQPTPPSTEKVTPEAEKPVPEKPVEPKFVTPTLKTYTPAQ
                     PKVKPHVSIPEKINYSVSVHPVLVPAANPSKAVIDEAGQSVNGKTVLPNAELNYVAKQ
                     DFSQYKGMTASQGKIAKNFVFIDDYKDDALDGKSMKVNSIKASDGTDVSQLLEMRHVL
                     STDTLDEKLQTLIKEAGISPVGEFYMWTAKDPQAFYKAYVQKGLDVTYNLSFKVKKEF
                     TKGQIQNGVAQIDFGNGYTGNIVVNDLTTPEIHKDVLDKEDGKSINNGTVKLGDEVTY
                     KLEGWVVPTGRSYDLFEYKFVDQLQRTHDLYLRDKVVAKVDVTLKDGTVIKKGTNLGE
                     YTETVYNKKTGLYELVFKKDFLEKVARSSEFGADDFVVVKRIKAGDVYNTADFFINGN
                     KVKTETVVTHTPEKPKPVEPQKATPKAPAKGLPQTGEASVAPLTALGAIILSAIGLAG
                     FKKRKEN"
     gene            complement(3691..5952)
                     /locus_tag="VBE22_001640"
                     /pseudo
     CDS             complement(3691..5952)
                     /locus_tag="VBE22_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(5968..6231)
                     /locus_tag="VBE22_001641"
     CDS             complement(5968..6231)
                     /locus_tag="VBE22_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203080.1"
                     /translation="MGVKSNEELKREQYLAEFSRVVTNFKWNASRIYFGDIIAIKLCF
                     KEALKQIDILHERAILRRDEVSTFYYWAKRNINNVYQELIEKK"
     gene            complement(6251..6712)
                     /locus_tag="VBE22_001642"
     CDS             complement(6251..6712)
                     /locus_tag="VBE22_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203081.1"
                     /translation="MGYRLNRYVCVRKDVFLALVMLLVTLIVSLVFLLKSDGISFFSD
                     QYYRSQLTAKEAKNVINKNYNIVFYKKSCPYCKIAKKEILEKSKGSDITTFFADVETV
                     SGKELVRKYRISKAPSIVAVRGDNLKVNLYAKDDEDGRMIVEKKVIKDSFR"
     gene            complement(6697..7008)
                     /locus_tag="VBE22_001643"
     CDS             complement(6697..7008)
                     /locus_tag="VBE22_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203082.1"
                     /translation="MFDLLKKQFNSFRLKKVLMDKGIKNYVVLYFKDNEKALCIVRNG
                     KKYNRCYLLKLSFYDYSIVKSYVADGDFLIYKGICKTGMVAYLLDNRKKWKSVEVWDI
                     D"
     gene            complement(7001..>7374)
                     /locus_tag="VBE22_001644"
     CDS             complement(7001..>7374)
                     /locus_tag="VBE22_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7203083.1"
                     /translation="QLITYLAITSRTYDRYEDGSRKPDIETLVKIADYYCCSLDVLVS
                     RNYTEDFVDRSLVVSYRDLKELGLNDKIAHGLIKQVYDGYSAEKIPLVDSIKKLKFIY
                     KKDLVSCLNDIMKNLEEKKYV"
BASE COUNT         2123 a         1392 c         1050 g         2809 t
ORIGIN      
        1 tccatcattt ttaacaaatc taaatagtaa taactataca cgcctacagg tgtcaaaaac
       61 aaaataaata gaaaagaaaa aaggtaataa ctcatatacc tcatatatgg ttttacctta
      121 atccctctat attgtggtat taaacgagcc aaattacccc tcctttaccg tttatttatt
      181 aactttagat gcttcatttt ttgatgcgtt tccattattt gcaacatcct ttttcttaag
      241 tgatgatgca aaaattttaa acataccacc tgaaacgtta gaccatgtca caacaacatc
      301 acttaattca atagggtcac ccaatttaag acctaaatcc tcgattgcat aagaaggcat
      361 atcagtaatt gaaacaaaaa ttgtttcatt ttgagcagta ctaaatactg caatatcatt
      421 ccacttcact tcacctgttg gtttttgtgg ataacgacca aatcgatctg gtggttgtgt
      481 cgtatcatct tccgtaaatt gaactggttc ttcatatcgt ctccaagtta aatctcctaa
      541 caaaacattt acatccaatc catcattaaa ggtacgatat tccttatctg caatttgtaa
      601 tttttctcca aagtttaaac caatttttgc catatttcta atcctctact ttccttattt
      661 cacttcaata agcttttcag cccatacatt ttttgcaggt tcaatatttc taccattaat
      721 tgtattgtca ataaacaata acggtttttc aacagttacc atttttccat ttccaatcac
      781 acgtgaagat gctggtgtat aaacataaaa cttaccatgt ttttcattaa acaaaatgtg
      841 tcttgtataa gacccttttt taccatttaa ttgagcaggt ccagcttcaa atccttcata
      901 aattgcatct ccaataaaat ttggaagtaa cactaatgtt tcttgtttca taatttcctc
      961 ttttctaaaa ataagaagca ctttttcaag tgcttctgaa tattgacttt ttaattttct
     1021 ttgcgttttt taaatcctgc taaaccgata gctgacaaga tgattgctcc aagtgctgta
     1081 agaggggcaa cgcttgcttc accagtttga ggtaatcctt tagcaggagc ttttggagtt
     1141 gctttttgtg gttcaactgg ttttggtttc tcaggagtat gtgttaccac agtttcagtt
     1201 ttaaccttat tcccattgat aaaaaagtct gcggtattgt aaacatcacc tgctttgata
     1261 cgtttaacca ccacaaaatc atctgcccca aattcactag aacgtgcaac tttttcaagg
     1321 aagtctttct tgaagactaa ttcatagaga cctgtctttt tgttgtaaac tgtttctgtg
     1381 tactctccta aattagtccc ttttttaatg actgtaccat cttttaatgt cacatcaact
     1441 ttagcgacca ctttatcacg taagtaaaga tcatgtgtgc gttgtaattg atccacaaat
     1501 ttgtattcaa atagatcata agaacgacct gttggaacta cccaaccctc taatttgtaa
     1561 gtaacttcat cacctaattt gacagtacca ttgttgatag acttgccatc ttctttgtct
     1621 aacacatctt tatgtatttc tggagttgtc aaatcattga ctacaatatt acctgtatat
     1681 ccattgccaa agtcgatttg tgcaacacca ttttgaattt gacctttagt aaactctttt
     1741 ttaactttaa atgataagtt ataagtaaca tctagacctt tttgaacata agctttataa
     1801 aaagcttgtg gatcttttgc agtccacatg taaaactcac caacaggact gattcctgct
     1861 tctttaataa gcgtttgaag tttctcatct aaagtatccg tagaaagcac atgtcgcatt
     1921 tctaagagtt gtgacacatc tgtgccatca gatgctttga ttgagttcac tttcatagac
     1981 ttaccatcta gtgcgtcatc tttataatca tcaataaaga caaagttttt agctatttta
     2041 ccttgtgatg cagtcattcc tttatattgg ctaaagtctt gtttagcaac gtaatttaat
     2101 tcagcatttg gtaatacggt tttaccatta acagattgac ctgcttcatc aatgacagct
     2161 tttgaaggat tagcagctgg aactaaaaca ggatgaacac taactgagta gttaatcttt
     2221 tcaggaatag acacatgtgg tttcactttt ggttgagctg gagtgtaagt ttttaatgta
     2281 ggtgttacaa atttaggctc aactggtttt tcaggtactg gtttttctgc ttcaggagtc
     2341 actttttctg ttgacggtgg tgttggctgt tttggtataa caacgttagg aacaacatca
     2401 ttattattaa cataagacac taaagcagac ccatgtgtgt catgtaccca gtcatcatga
     2461 atcttaaaga cttcaacgct tccactagaa ccatgtggtt taagctcata tttaagattg
     2521 agttgtaaag ttttgtttaa agtttctgca aaagttttgt taccattatg tttcttctca
     2581 aaaatgattt gtccgtcttt agttaagact ctgaaagcaa tattttgatt aacagcagca
     2641 ttttgagcaa cattacgata ctcgtacttg taaagagtga tagagtcaac aaatgctgtc
     2701 acagagccat ctttgttagc aacataatga tgattaactt tacctgtcaa tttcccatag
     2761 ccgattgtct tcacaccatc gatcatatca tggttaaact cgaaacttcc gtcaggttta
     2821 gtttttgcag tcatgattga gtttccatat tcagcgttat caacaccttt agcttgagct
     2881 ttgatatcaa aagcaacatt attaatagac tgtttatctg cttctgcttt agcttttaga
     2941 gcttgattat aaagtgctaa tttagcttga tagtcttttt gtaactgtgc gttagtcgca
     3001 tctgctttag cttgtgcatc tttattacgt tgacgtacac ctgcattata agcttcaact
     3061 gatttgattt tcgctaattt agcttgataa tctttttgta attgtgcatt agtcgcatct
     3121 gctttagctt gtgcatcttt gttttcagca tcaatttttg ctttagtttg tgcatttaac
     3181 tgacttacac gttgagactc tgctttgttt tctgcgtcaa tttttgcatt ttcagcagta
     3241 gcatctttaa tagcttgtgt attagcttgt gcttttgcag taacatcttt aactgtttgt
     3301 gtttgattag ctaaatcagc ttgtgcatca gtagttgtcg catgattaac tgcagctgtg
     3361 gttgtaacag ctacagctgc ttttttagct gtagctactg ctttatcaag ttgagttgat
     3421 gttactgttt ctgcttgatt ttgcgttact gtttgagttg ttgcttgagt tgtaacttga
     3481 tcagcagaca cttgactagt agctaatgta aatgcacctg caagtgcaat cccacatact
     3541 aaaccatatg cttttgattt tctaaaaaat ccgtgacctt ttgtgttttg atttttatac
     3601 atattttctc ctttgatttt aattttttat ataaaaaaaa gaaggcgatc ttgccctcaa
     3661 aatatcacac ataaatcatc ctccttatta ttaaatatta ttcttattat ttttacgtac
     3721 acctaaaaac ccacttaaag ataacagcat acctaaaact gcaattaaac ctgtcttagc
     3781 atcaccagtt gatggcaaga cactagattt tacaactgat acttgttcaa catcaactgt
     3841 cttagttaca acgtctttct tatcgttagc aacaactatt tgttgtgctt taacagcttc
     3901 ttgcagtttt ggtttagaag taatataact agtaatttta cctgtttcat caactactgc
     3961 tactgcttct tgtcttactt tctcaatttg agctttttct tctgctaatt tcagtttttc
     4021 tttagcttct aaaactgact tataagctga taaaactttt tgatattgtg attgcgcatc
     4081 tactgcttta gtttttaact cttgtaaata agctacttct ctttgtaact tagcaatctt
     4141 atttgctaag ttatctttag cttgtgctac atcagactgt actttaagca aattagcttg
     4201 tgcattgcgt agatttgtaa gataagcagt tttttgtgtt agattatctt tagcttgctt
     4261 gtaatcttga tttgcttgtg ctacttgttg acttgcttct gctaacactt gttttaattg
     4321 attaagttta gttgtatttg ttgtcaattt aacctgtgca tctgctaatt gcttttgagc
     4381 atctgttaat ttagactctg cttttattaa gtcagcttgt tttttaacat tatcttgatt
     4441 agctaattta actgcttctt gtgctttagt gagtgcatct tgagcatctt ttaaattaga
     4501 ctatgcttta atcaaattag cttgtgcaat cggtgtctgc atagttactg cattaagacc
     4561 tattgcttta gcttgtgcat catttaattg tctttgagca acttgttgac ttgctaaagc
     4621 ttgtgactgc ttagttaagg cattagccat atctttagtc ataattgaga aaaacggtac
     4681 acctacacca gcagtatcaa tactatctcg tgactgttca ttccaaatgc ggtcaacact
     4741 gtaaaaacca ttgttatcac ggttatacgg atcataaaca tacgtattgc catttgaact
     4801 acctctcaat acaatttcgt gactgtattg aggtccccaa ggactgaatt tattattttg
     4861 tacagcagca acaacatgat gacctgcttt caaagcttct tttatagcat tttgacttgc
     4921 taaatgagtc acaacatagc caaacgcttt agtagcttca acaatacctc ttccacttgt
     4981 tccaccaaaa ttcttattaa attcatttgt ttggttaaaa agataatccg caacttcctt
     5041 tggtgtaact gtcttgcgtg ataaatcaga ataaaccatc gctaaagccg aaggtacaca
     5101 tccagttgaa gcaaaagtat gccctccata tacgctagcc catgcgccgt cacgttgatt
     5161 gtaataatga ctttgcccta gagcagtttt aaacttattc tggttaacag tagcattagc
     5221 ttgtgctaac tgtgcatctt taacagataa attttgtttt gcgttatcaa gattttgttg
     5281 tgcttttgct ttttgattag taagtgtttt atcagtcgct tgtgcttttg taagtgcatc
     5341 tgtcgcagtt ttaacatcag ctgtcttagt tgtgatgttt gactgtgctt tatttaaatc
     5401 actattaact gtagtattag tcagagcatc tttagctcta tccactgatg attgagcttg
     5461 tttaacatca gttgtcttag ttgcaacgtc ttgcttatcg tgtgcaacaa ctatttgttg
     5521 tgctttaaca gcttcttgcg ctttgacttg ttcagtttca gcagtttttt gtttagctcc
     5581 aatctgtgac aacatttgtt tagctatatc aattgtttgt tgtgctttaa ctttattttc
     5641 ttgagttgac tcttgagcta gtttttgtgt tgcattcaac tttttagttg cattgactag
     5701 agtttgttga gcttgatttt gttgattttg tgcagtatta actacatttt gttgtgtttc
     5761 aacatcactc gttgctctat cagactttgc ttttgcactg tcaacttgtt cttgtgttac
     5821 ttctgtaatg tgattagttt gattcgcagt tgtttctaac gataatgatt taacttcgtc
     5881 agcatttact gctacgctta ctgtacttaa tgttgcaatt gcaatacctg ataaaacttt
     5941 agtttgtttc ataaaaatct cctcaattta cttcttttca atcaattctt gataaacatt
     6001 atttatattt cttttagccc agtagtaaaa tgtactaact tcatctctac gtaaaatagc
     6061 tctttcatgt aatatatcta tttgttttaa agcttcttta aaacaaagtt ttattgcaat
     6121 tatatctccg aaatatattc tactagcatt ccacttaaaa ttagtaacca ccctactaaa
     6181 ctctgctaaa tactgctctc ttttcaattc ctcattactt tttaccccca tgataaatac
     6241 ctttctataa ctatctaaaa ctatctttta ttactttctt ttctacaatc atacgaccat
     6301 cctcatcatc ttttgcatac aaattaactt tcaagttgtc tcctcttaca gcaactatgc
     6361 ttggcgcttt ggatattcta tatttacgta ctagctcctt gccactaaca gtctccacgt
     6421 cagcaaaaaa agtagttata tcactacctt tacttttctc taaaatttct tttttcgcta
     6481 ttttgcagta agggcaactc tttttataaa aaacaatatt atagttttta ttaatcacat
     6541 ttttagcttc tttagcagtc aattgagacc tatagtactg atcactaaaa aatgagatgc
     6601 catcactctt taataaaaaa acaagtgata caattaacgt cactagtagc atcactaaag
     6661 ctaaaaagac atccttccta acacaaacat acctattcaa tctatatccc atacttcaac
     6721 cgacttccat ttttttctat tatctaaaag gtaagcaacc atacctgttt tacaaatacc
     6781 cttataaatt aaaaaatcac cgtcagccac atacgattta acaatagaat aatcgtaaaa
     6841 agataatttt aataaatagc aacgattata ttttttccca tttcttacaa tacataacgc
     6901 tttttcatta tctttaaagt acaatacaac ataattttta attcccttat ccatcaaaac
     6961 tttttttaac ctgaaagaat taaattgttt ttttaacaaa tcaaacatac tttttttcct
     7021 ccaaattttt cataatatcg tttaaacaac taaccaaatc ttttttataa ataaatttaa
     7081 gttttttaat agaatcgacc aaaggaattt tctctgctga ataaccatca tagacttgtt
     7141 ttattaaacc atgcgcaatt ttatcattta aacccaactc ctttaaatca cgataagaaa
     7201 caactagcga cctatcaaca aaatcctcag tataatttct acttactaaa acatcaagcg
     7261 aacaacaata ataatcagct atctttacta atgtttcaat atctggctta cgtgaaccat
     7321 cttcatatct atcataagtt cttgaagtga tagctaaata ggtaatcaac tgtg
//
LOCUS       DATRTN010000080         7285 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.080, whole genome shotgun sequence.
ACCESSION   DATRTN010000080 DATRTN010000000
VERSION     DATRTN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.080"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            58..387
                     /gene="rnpA"
                     /locus_tag="VBE22_001645"
     CDS             58..387
                     /gene="rnpA"
                     /locus_tag="VBE22_001645"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEO7203084.1"
                     /translation="MKKTYRVKSDKDFQMIFSRGKNVANRKFVIYYLEKEQKHFRVGI
                     SVSKKLGNAVVRNAIKRKIRHVLLSQKTALQDYDFVVIARKGVEELDYQALEKNLIHV
                     LKIAGLI"
     gene            400..1215
                     /locus_tag="VBE22_001646"
     CDS             400..1215
                     /locus_tag="VBE22_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO7203085.1"
                     /translation="MKKKLKTFSLILLTGSLLVACGRGEVSSHSATLWEQIVYAFAKS
                     IQWLSFNHSIGLGIILFTLIIRAIMMPLYNMQMKSSQKMQEIQPRLKELQKKYPGKDP
                     DSRLKLNDEMQSMYKAEGVNPYASVLPLLIQLPVLWALFQALTRVSFLKVGTFLSLEL
                     SQPDPYYILPVLAALFTFLSTWLTNKAAVEKNIALTLMTYVMPFIILVTSFNFASGVV
                     LYWTVSNAFQVFQILLLNNPYKIIKVREEAVRVAHEKEQRVKRAKRKASKKRK"
     gene            1223..2044
                     /locus_tag="VBE22_001647"
     CDS             1223..2044
                     /locus_tag="VBE22_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEO7203086.1"
                     /translation="MVLFTGATVEEAIEKGLQELNISRLRAHIKVVSREKKGFLGFGK
                     KPAKVEIEGITDEVTDINESVVLKNIKNVPSSVDVVEEYIEEVDETLEKEDVSQPELP
                     KIDDKNVVTTSEAIEKIDLLPNIEVAAAQVTKYVENIIYEMDLDATIETTTSKRQISL
                     QIETPEAGRIIGYHGKVLKSLQLLAQNYLHDRFSKSFSVSINVHDYVEHRTETLIDFS
                     KKIARRVLETNEPYHMDPMSNSERKTVHKTIATIEGVESYSEGNDPNRFVVVTKK"
     gene            2384..3932
                     /locus_tag="VBE22_001648"
     rRNA            2384..3932
                     /locus_tag="VBE22_001648"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            3987..4059
                     /locus_tag="VBE22_001649"
     tRNA            3987..4059
                     /locus_tag="VBE22_001649"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4020..4022,aa:Ala,seq:tgc)
     gene            4214..7116
                     /locus_tag="VBE22_001650"
     rRNA            4214..7116
                     /locus_tag="VBE22_001650"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            7191..>7285
                     /gene="rrf"
                     /locus_tag="VBE22_001651"
     rRNA            7191..>7285
                     /gene="rrf"
                     /locus_tag="VBE22_001651"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2253 a         1346 c         1893 g         1793 t
ORIGIN      
        1 agtcttttag gttatttatg gtataataaa accaatttag ataaatggag cactattttg
       61 aaaaaaacct atcgggtaaa aagtgataag gattttcaaa tgattttcag cagaggtaaa
      121 aatgttgcta atcgaaaatt tgttatctac tatttagaaa aagaacaaaa acactttcgt
      181 gtgggaatct ctgttagtaa gaaattagga aatgctgttg ttcgtaacgc tattaaaaga
      241 aaaattaggc atgttttact ctctcagaaa acagcgctgc aagattatga ttttgtcgtg
      301 attgcgcgta aaggtgtaga ggaattagac tatcaagcat tagaaaaaaa tcttatacac
      361 gttttaaaaa tagcaggact catctagaaa ggtataaaat tgaagaaaaa acttaaaaca
      421 tttagcttaa tattgctgac aggttcttta ttggttgctt gcggacgagg agaagtatct
      481 agtcattcag ccacattatg ggagcaaata gtttatgcat ttgctaaatc aatacaatgg
      541 ctctccttta accattcaat tggtttggga ataatcttat tcaccctaat aatcagagcg
      601 attatgatgc cgctttataa tatgcaaatg aaatccagcc aaaagatgca ggaaattcag
      661 ccaaggctca aggaattaca aaaaaaatat ccagggaaag atcctgatag ccgtttaaag
      721 ctaaatgatg aaatgcaatc tatgtataaa gctgagggag ttaacccata tgcaagtgta
      781 ttacctcttc taattcagtt acctgtccta tgggctcttt tccaagcttt gactagggtt
      841 tctttcttaa aagtaggaac atttttgagc ctagaattat cacagccgga cccatattat
      901 attttacctg tactggcggc cttatttacc tttttatcaa cttggttaac taacaaagca
      961 gctgttgaaa aaaatattgc tttaactctt atgacttatg ttatgccgtt tatcatattg
     1021 gtaacaagtt ttaactttgc tagtggtgtt gttttatact ggacggtgtc taatgctttt
     1081 caagtatttc aaattctatt attgaacaac ccatacaaga ttataaaggt tcgagaagaa
     1141 gcagtaagag tggcacacga aaaagaacaa agagttaaac gtgctaagag aaaagcaagt
     1201 aagaaaagaa agtaggaaaa acatggtatt atttacagga gcgactgtag aagaagctat
     1261 tgaaaaagga ctacaagagt taaacatttc acgattgagg gcacatataa aagttgtatc
     1321 acgtgagaaa aaaggttttt tgggctttgg taaaaagcca gcaaaagttg aaatagaagg
     1381 tatcaccgac gaagtaactg atattaatga atcggtagtg ttaaaaaata ttaagaatgt
     1441 accgtctagc gtagatgtgg tggaagaata tatagaggag gttgatgaaa cgcttgaaaa
     1501 agaagacgta tcacaaccag aacttccaaa aattgatgat aaaaacgttg taactacaag
     1561 tgaagctatt gaaaaaattg acctacttcc taatattgaa gtagcagctg ctcaggtgac
     1621 taaatacgtt gaaaatatca tctatgagat ggatttagat gcaacaatag aaactacgac
     1681 aagtaagcga caaataagtc tccaaataga aacgccagaa gctgggcgca tcattggtta
     1741 tcatgggaaa gtattaaaat cgttgcagtt gttagcacaa aattatcttc atgaccgctt
     1801 ttctaagagt ttttcagtat ccattaatgt ccatgactac gttgagcatc gtacagaaac
     1861 tttaattgat ttttctaaaa aaatagctag acgtgtttta gagacaaatg aaccttacca
     1921 tatggatcct atgagtaaca gtgagcgaaa aactgttcat aaaacaattg caactataga
     1981 gggtgttgaa agttattctg aaggtaacga tcctaataga tttgtagtag ttactaagaa
     2041 gtaaaaagag gtttcacctc tttttttctt gcgcccgatc tacattttca gaaaatgtag
     2101 aaaaagtatg aaaaaagagt tgacaaagta agagatgatg tagtaaacta aataagttgt
     2161 ctcgagagag cagcttctag taacgcgtag ttaacaactt aaaaaagtag ttgacaaagc
     2221 agaataagtt tgctagaata tagaagttgt ctcaagagag gcaaagacct ttgaaaactg
     2281 aacaagacga acgaatgtgc agggtggatt tacattgtaa atccgtcaat gacaaaaaaa
     2341 caataaatct gtcagcgaga cagaaaagag caaagctcaa acttttaatg agagtttgat
     2401 cctggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtagaac gctgaggttt
     2461 ggtgtttaca ctagactgat gagttgcgaa cgggtgagta acgcgtaggt aacctgcctc
     2521 atagcggggg ataactattg gaaacgatag ctaataccgc ataagagtaa ttaacacatg
     2581 ttggttattt aaaaggagca attgcttcac tgtgagatgg acctgcgttg tattagctag
     2641 ttggtgaggt aaaggctcac caaggcgacg atacatagcc gacctgagag ggtgatcggc
     2701 cacactggga ctgagacacg gcccagactc ctacgggagg cagcagtagg gaatcttcgg
     2761 caatggacgg aagtctgacc gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa
     2821 agctctgttg ttagagaaga acgttggtag gagtggaaaa tctaccaagt gacggtaact
     2881 aaccagaaag ggacggctaa ctacgtgcca gcagccgcgg taatacgtag gtcccgagcg
     2941 ttgtccggat ttattgggcg taaagcgagc gcaggcggtt ctttaagtct gaagttaaag
     3001 gcagtggctt aaccattgta cgctttggaa actggaggac ttgagtgcag aaggggagag
     3061 tggaattcca tgtgtagcgg tgaaatgcgt agatatatgg aggaacaccg gtggcgaaag
     3121 cggctctctg gtctgtaact gacgctgagg ctcgaaagcg tggggagcaa acaggattag
     3181 ataccctggt agtccacgcc gtaaacgatg agtgctaggt gttaggccct ttccggggct
     3241 tagtgccgca gctaacgcat taagcactcc gcctggggag tacgaccgca aggttgaaac
     3301 tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac
     3361 gcgaagaacc ttaccaggtc ttgacatcct tctgaccggc ctagagatag gctttctctt
     3421 cggagcagaa gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt
     3481 taagtcccgc aacgagcgca acccctattg ttagttgcca tcattaagtt gggcactcta
     3541 gcgagactgc cggtaataaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct
     3601 tatgacctgg gctacacacg tgctacaatg gttggtacaa cgagtcgcaa gccggtgacg
     3661 gcaagctaat ctcttaaagc caatctcagt tcggattgta ggctgcaact cgcctacatg
     3721 aagtcggaat cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt
     3781 gtacacaccg cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg aggtaacctt
     3841 ttaggagcca gccgcctaag gtgggataga tgattggggt gaagtcgtaa caaggtagcc
     3901 gtatcggaag gtgcggctgg atcacctcct ttctaaggat aaggaaacct gccatttgcg
     3961 tcttgtttag ttttgagagg tcttgtgggg ccttagctca gctgggagag cgcctgcttt
     4021 gcacgcagga ggtcagcggt tcgatcccgc taggctccat tgaatcgaaa ggttcaaatt
     4081 gttcattgaa aattgaatat ctatatcaaa ttccacgatc tagaaataga ttgtagaaag
     4141 taacaagaaa ataaaccgaa aacgctgtga atatttaatg agttttctag ttttaaagaa
     4201 actaggttaa taaggttaag ttaataaggg cgcacggtgg atgccttggc actagaagcc
     4261 gaagaaggac gtgactaacg acgaaatgct ttggggagct gtaagtaagc gctgatccag
     4321 agatgtccga atgggggaac ccactagtta atgactagta tccatatctg ttaaggatat
     4381 gagaaggaag acgcagtgaa ctgaaacatc taagtagctg caggaagaga aagcaaaagc
     4441 gattgcctta gtagcggcga gcgaaacggc aggagggcaa accgaagtgt ttacacttcg
     4501 gggttgtagg actgcgatat aggattaatc attatagaag aattacctgg gaaggtaagc
     4561 caaagagagt aacagcctcg tatttaaaat gatagattaa ccttagcagt atcctgagta
     4621 cggcgagaca cgcgaaatct cgtcggaatc tgggaggacc atctcccaac cctaaatact
     4681 ctctagtgac cgatagtgaa ccagtaccgt gagggaaagg tgaaaagcac cccgggaggg
     4741 gagtgaaata gaacctgaaa ccgtgtgcct acaacaagtt cgagcccgtt aatgggtgag
     4801 agcgtgcctt ttgtagaatg aaccggcgag ttacgatatg atgcgaggtt aagttgaaga
     4861 gacggagccg tagggaaacc gagtcttaat agggcgccat agtatcatgt tgtagacccg
     4921 aaaccatgtg acctacccat gagcaggttg aaggtgaggt aaaactcact ggaggaccga
     4981 accagggcac gttgaaaagt gcttggatga cttgtgggta gcggagaaat tccaaacgaa
     5041 cttggagata gctggttctc tccgaaatag ctttagggct agcgtcgatg ttaagtctct
     5101 tggaggtaga gcactgtttg ggtgaggggt ccatcccgga ttaccaatct cagataaact
     5161 ccgaatgcca acgagatata atcggcagtc agactgcgag tgctaagatc cgtagtcgaa
     5221 agggaaacag cccagaccac cagctaaggt cccaaaatat atgttaagtg gaaaaggatg
     5281 tggggttgca cagacaacta ggatgttagc ttagaagcag ctattcattc aaagagtgcg
     5341 taatagctca ctagtcgagt gaccctgcgc cgaaaatgta ccggggctaa aacatattac
     5401 cgaagctgtg gatgacacaa aagtgtcatg gtaggagagc gttctatgtg tgaagaaggt
     5461 gtaccgtgag gagcgctgga acgcatagaa gtgagaatgc cggtatgagt agcgcaagac
     5521 aggtgagaat cctgtccacc gtaagactaa ggtttccagg ggaaggctcg tccgccctgg
     5581 gttagtcggg acctaaggag agaccgaaag gtgtatccga tggacaacag gttgatattc
     5641 ctgtactaga gtatatagtg atggagggac gcagtaggct aactaaacca gacgattgga
     5701 agtgtctggt caaacagtga ggtgtgatat gagtcaaatg cttatatctt taacattgag
     5761 ctgtgacgag gagcgaagtt tagtagcgaa gttagtgatg tcacactgcc aagaaaagct
     5821 tctagcgata catatactct acccgtaccg caaaccgaca caggtagtcg aggcgagtag
     5881 cctcaggtga gcgagagaac tctcgttaag gaactcggca aaatggcccc gtaacttcgg
     5941 gagaaggggc gctgatttta gatcagccgc agtgaatagg cccaagcaac tgtttatcaa
     6001 aaacacagct ctctgctaaa tcgtaagatg atgtataggg ggtgacgcct gcccggtgct
     6061 ggaaggttaa gaggagtgct tagcgtaagc gaaggtatga attgaagccc cagtaaacgg
     6121 cggccgtaac tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacccg
     6181 cacgaaaggc gtaatgattt gggcactgtc tcaacgagag actcggtgaa attttagtac
     6241 ctgtgaagat gcaggttacc cgcgacagga cggaaagacc ccatggagct ttactgcagt
     6301 ttgatattga gtatctgtac cacatgtaca ggataggtag gagcctatga taacgggacg
     6361 ccagtttcgt ttgaggcgtt gttgggatac tacccttgtg ttatggctac tctaacccag
     6421 ataggcaatc cctatcggag acagtgtctg acgggcagtt tgactggggc ggtcgcctcc
     6481 taaaaggtaa cggaggcgcc caaaggttcc ctcagactgg ttggaaatca gtcgtagagt
     6541 gtaaaggtat aagggagctt gactgcgaga gctacaactc gagcagggac gaaagtcggg
     6601 cttagtgatc cggtggttcc gtatggaagg gccatcgctc aacggataaa agctaccctg
     6661 gggataacag gcttatctcc cccaagagtt cacatcgacg gggaggtttg gcacctcgat
     6721 gtcggctcgt cgcatcctgg ggctgtagtc ggtcccaagg gttgggctgt tcgcccatta
     6781 aagcggcacg cgagctgggt tcagaacgtc gtgagacagt tcggtcccta tccgtcgcgg
     6841 gcgtaggaaa tttgagagga tctgctccta gtacgagagg accagagtgg acttaccgct
     6901 ggtgtaccag ttgtcttgcc aaaggcatcg ctgggtagct atgtagggaa gggataagcg
     6961 ctgaaagcat ctaagtgcga agcccacctc aagatgagat ttcccatgat tttatatcag
     7021 taagagccct gagagatgat caggtagata ggttaggagt gtaattgtgg tgacacattt
     7081 agcggactaa tactaatagc tcgaggactt atccaaaaag agatattgac aacgttacgg
     7141 tttcttgtta gactatagat attcaatttt gagtgaataa gactcaatag ttaagtgata
     7201 attgcctagg agatacacct gttctcatgc cgaacacaga agttaagccc tagaacgccg
     7261 gaagtagttg ggggttgccc cctgt
//
LOCUS       DATRTN010000081         7256 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.081, whole genome shotgun sequence.
ACCESSION   DATRTN010000081 DATRTN010000000
VERSION     DATRTN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.081"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            98..2335
                     /gene="metE"
                     /locus_tag="VBE22_001652"
     CDS             98..2335
                     /gene="metE"
                     /locus_tag="VBE22_001652"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEO7203087.1"
                     /translation="MVKVSNLGYPRLGEQREWKQAIEAFWAGNLEQKDLEKQLKQLRI
                     NHLKKQKEAGIDLIPVGDFSCYDHVLDLSFQFNVIPKRFDEYERNLDLYFAIARGDKD
                     NVASSMKKWFNTNYHYIVPEWEVETKPHLQNNYLLDLYLEAREVVGDKAKPVITGPIT
                     YVSLSSGIVDFEATVQRLLPLYKQVFQDLIDAGATYIQIDEPIFVTDEGELLVDIAKS
                     VYDFFAREVPQAHFIFQTYFESAVCLDKLSKLPVTGFGLDFIHGRAENLAAVKQGLFR
                     EKELFAGIVNGRNIWAVNLEETLALLEEIGPFVKRLTLQPSSSLLHVPVTTKYETHLD
                     PVLKNGLSFADEKLKELELLASAFDGNKTKGYHEALSRFSALQAADFRHVALESLAEV
                     KLERSPYKLRQALQAEKLQLPILPTTTIGSFPQSPEIRKKRLAWKRGNLSDSDYKDFI
                     KTEIRRWIAIQEDLDLDVLVHGEFERVDMVEFFGQKLAGFTTTKLGWVQSYGSRAVKP
                     PIIYGDVKHIQPLSLEETVYAQSLTKKPVKGMLTGPITITNWSFERDDISRSDLFNQI
                     ALAIKDEIQLLEQSGIAIIQVDEAALREGLPLRQQKQQAYLDDAVAAFKIATSSVKDE
                     TQIHTHMCYSKFDEIIDSIRALDADVISIETSRSHGDIIESFETAVYPLGIGLGVYDI
                     HSPRIPTKEEIIVNIQRSLKCLSKEQFWVNPDCGLKTRREAETIAALEVLVSATKEVR
                     QQLDN"
     gene            2380..4224
                     /locus_tag="VBE22_001653"
     CDS             2380..4224
                     /locus_tag="VBE22_001653"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEO7203088.1"
                     /translation="MSKFLEKLKTDILVADGAMGTLLYTYGLDTCHESYNVTHPEKVL
                     AIHQAYIEAGADVIQTNTYGAQRHRLKNYGLEDQVVSINQAAVNIAHQATLGKETFIL
                     GTVGGFRSQRQCDLTLDNIVEETLEQVEALLATGQLDGLLFETYYDIEEITTVLKIVR
                     EMTDLPIITNISLHEAGVTSNGKPIVEALSQLVMLGADVIGLNCHLGPYHMIQSLKQV
                     PLFAQSYLSVYPNASQLSLDGENSQYQFSQNSEYFGKSAELLVAEGVRLIGGCCGTTP
                     DHIRAVKRSIRGLKPIERKVVTPIIPVKDFVRRIRRTDTLVDKVKKEVTIIAELDPPK
                     HLDIVQFQKAIRAIDQKGIAAITLADNSLSNTRICNLSIASLLKDEISTPFLLHIACR
                     DHNLIGLQSRLLGMELLGFNHILAITGDPTKLGDFPGATSVYDVTSFKLLSLIKQLNQ
                     GLSYSGASLRRPTDFTVAAAFNPNVKNLTRTVKLIEKKVASGADYFMTQPIFDHSVLK
                     ELADLTKTVEQPFFIGIMPITSYNNAVFLHNEVPGIKLSESFLSALEKVKDDKEACLT
                     LALNESKSLIDEALNYFNGIYLITPFLRYDLTLELIDYIQKKQVRKSS"
     gene            4571..5278
                     /locus_tag="VBE22_001654"
     CDS             4571..5278
                     /locus_tag="VBE22_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEO7203089.1"
                     /translation="MVCVDFIDQKVYSEAEGLFVSDLDLGIKGLIRARYPFALDSDFI
                     SYGNLSHYCMNYLDEIVQRANQKNEKIKYNVTTLMKEEEKPFNVEERLNKQATIGQRI
                     ADDVARFGGSWTFIIVFVSIMAIWMLVNIMKPFGIQFDPYPFILLNLALSTIAAIQAP
                     LIMMSQNRAADYDRLQARNDFNVNKTSELEIRLLHEKIDHMVQQDQFELLEIQKLQTE
                     MLVSLGNQLAQLKQLQK"
     gene            complement(5443..6528)
                     /locus_tag="VBE22_001655"
     CDS             complement(5443..6528)
                     /locus_tag="VBE22_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO7203090.1"
                     /translation="MTTNVMANYSYGEDCFKEIPSVLATYRIQSIAFVGGKRALDSSE
                     AEVRSILEEANIKVTGSFVYGTDSTQSNIDKLVANPQVQAADAILGFGGGKALDTAKM
                     VAKELGKNSFTIPTICSNCSAGTAIAVVYNDDHSFLRYGYPESPLHIFINTRIIAQAP
                     SKYFWAGIGDGISKAPEVERATLEAKTNKLPHTAVLGQAVALSSKEAFYQFGEQGLKD
                     VEANLASRAVEEIALDILISTGYASNLVNQPDFYYNSCHAHAFYYGTTAIQRQGEFLH
                     GVVVAFGVLVLHAYFNELEELEKVARFNKSLGLPTTLADVSLSEKDIPKIVEIAMTTN
                     EYKNTPFDPKMFAQAILAADAFGQTLA"
     gene            complement(6729..7256)
                     /locus_tag="VBE22_001656"
     CDS             complement(6729..7256)
                     /locus_tag="VBE22_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEO7203091.1"
                     /translation="MIGYSGSGKSTLARKLGNYYNCNVLHLDSIHFAPNWEERKYDDM
                     IDDVSNMLEKRTWIIEGNYKKLLYQERLADADEIIFFDFNRFNCLWRAFKRYCKFRGK
                     TRPDMANGCPEKLDFEFISWILKDGRSDKQKSNYKQVVEDYPQKIKILKHQRDLDQYL
                     KELPVASNTDPLKSL"
BASE COUNT         2323 a         1192 c         1403 g         2338 t
ORIGIN      
        1 aaatattaaa agctattgat attatagttt tcatggacta aaataattca taaaactaaa
       61 agggttagtt attagctata acttgatgga ggaaattatg gtaaaagttt caaatttagg
      121 gtatccacgt cttggtgaac agcgcgaatg gaagcaagcg atcgaagctt tctgggcagg
      181 gaatcttgaa caaaaagatt tagaaaaaca actaaaacaa ttacgtatca atcatttaaa
      241 gaaacaaaaa gaggcaggta ttgaccttat tccagtgggg gatttttctt gttatgatca
      301 tgttttggat ttgtcatttc aattcaatgt aatcccaaag cgtttcgatg agtatgagag
      361 gaatttagac ctttattttg ctattgcaag aggtgataaa gataatgtcg catcatctat
      421 gaaaaagtgg tttaatacca actaccacta catagtccca gaatgggagg ttgagactaa
      481 acctcacttg cagaataatt acttacttga tctttatcta gaagctaggg aagtagttgg
      541 tgataaagca aagccggtta tcactggtcc aataacctat gtttccttat catcaggaat
      601 tgtcgacttt gaagcgactg ttcagcggtt attaccactt tataagcagg tctttcaaga
      661 tctgatagat gcaggcgcca cctatattca gattgatgag ccgatatttg taactgatga
      721 aggtgaactt ttagtagata tagctaagtc tgtttatgat ttttttgcaa gagaagtacc
      781 acaagcccac ttcatctttc aaacctactt tgaatcagca gtctgtttag ataaactctc
      841 taagctgcca gtaacgggat ttggccttga ttttatacat ggtagggcgg aaaatttagc
      901 tgctgttaag caaggtctat tccgcgaaaa agaattattt gcaggaattg ttaatggtcg
      961 aaatatctgg gcagtaaatt tagaagaaac gttggcttta ttggaagaga taggtccctt
     1021 tgttaaacga ttgactcttc aaccttcttc aagtctttta catgtaccgg tgacgactaa
     1081 atacgaaaca catttagacc ctgtgttaaa gaatggctta tcatttgctg atgaaaaact
     1141 aaaggaatta gaactattag ctagtgcttt tgatggtaat aaaacaaagg gatatcacga
     1201 agctttatct cgtttttcag ctcttcaagc tgctgatttt cgtcatgtag cattggaatc
     1261 attagcagaa gtaaagcttg aacgaagtcc gtataaatta cgccaagctt tgcaagctga
     1321 aaaattacag ttaccgattt taccaacaac aactattgga tcctttcctc aatcacctga
     1381 aattaggaag aaacgccttg cttggaaaag aggaaatcta tctgactcag attataaaga
     1441 tttcataaaa actgaaatta gaagatggat tgctattcaa gaagatcttg atcttgatgt
     1501 gttagtacat ggcgaatttg agcgtgttga tatggttgaa ttttttggtc aaaagttagc
     1561 tggttttacg acaaccaaat taggctgggt acagtcttat ggttcaaggg cggtcaaacc
     1621 acctatcatt tatggtgatg tcaaacatat tcaaccctta agccttgaag aaacggttta
     1681 tgcccaaagt ttgactaaga aacctgttaa aggcatgttg acaggtccta ttactataac
     1741 gaactggtca tttgagcgag atgatattag ccgatctgat ctttttaatc aaattgcttt
     1801 ggctataaaa gatgagattc aacttttgga acaatcaggt attgctatta tacaagtgga
     1861 tgaagcagcc cttcgagaag gtttaccctt acgccagcaa aagcaacagg cttacttaga
     1921 tgatgctgtt gcggccttta aaattgcaac ttcatctgtg aaagatgaga cacaaattca
     1981 tacacatatg tgttattcaa aatttgacga aattattgat tctatccgtg cactagatgc
     2041 agatgttatt tctattgaaa cgagtagaag tcatggggac atcattgaaa gttttgaaac
     2101 agcagtttat cctctaggaa ttggcctggg tgtttatgat attcattccc ctcgcatacc
     2161 tactaaggaa gaaattattg tcaatattca acgatcacta aaatgtctat caaaagagca
     2221 attttgggta aaccctgatt gtggcttaaa aacacgccgt gaagcagaaa caattgctgc
     2281 cttggaggtt cttgtttcag ctaccaaaga ggttcgtcag caattagata attaataggt
     2341 taatcagtaa aggattgcca tgagggagga gtaaagaaga tgtccaagtt tttagagaaa
     2401 ttaaaaacag atattctagt agctgacggt gcaatgggaa ctctccttta tacttatggg
     2461 ctagatactt gtcatgaatc ctacaatgta acacatccag aaaaggttct agccattcat
     2521 caagcttata ttgaagcggg tgcagatgtt attcagacga atacctatgg ggctcaacgt
     2581 catcgattaa agaactacgg tttggaagat caggttgtct ctatcaatca agcagcagtt
     2641 aatatagctc atcaagcgac tttgggtaaa gaaacattta ttttaggtac tgttggaggt
     2701 tttcgttctc aaagacaatg cgatttaaca ttggataata ttgtagaaga aactttggaa
     2761 caagttgagg ctttattggc gacaggacag ctagacggat tattgtttga aacttattat
     2821 gatattgagg agataacgac agttttaaaa attgtgaggg agatgactga tctaccaatt
     2881 attactaata tttctcttca tgaagcaggg gtaacatcaa atggaaagcc tattgtagag
     2941 gcgctcagcc aattagttat gctaggtgca gatgtgattg gcttaaattg tcaccttgga
     3001 ccctatcata tgatccaatc attgaagcaa gttcctttat ttgcccagtc ttatttgtca
     3061 gtttatccta atgcaagtca gttatcttta gatggtgaga atagtcaata ccagtttagt
     3121 cagaattcag agtattttgg aaaatctgca gaacttctcg tagcagaagg agttaggctt
     3181 attggtggtt gttgtgggac aactccagat catattagag ctgtgaaacg atctattaga
     3241 ggattgaaac ctattgaacg aaaggttgtt actcctataa tacctgtaaa agattttgtt
     3301 aggcgtatca gacgaacaga tactctagtg gataaggtaa aaaaagaagt aacaattatc
     3361 gcagaattag atcctccaaa gcatttagat atcgttcagt ttcaaaaggc tatacgagcg
     3421 attgaccaaa aaggtattgc agcaattact ttagcagata attcactatc taatacacgt
     3481 atttgtaatt taagtatagc tagcttgctt aaagatgaaa ttagcacacc ctttttacta
     3541 catatagctt gtcgtgacca taatttaatt ggtttacagt ctagactgtt gggcatggaa
     3601 ttattaggtt ttaaccatat tttagccata acaggagatc cgactaaact tggtgatttc
     3661 ccaggagcga ctagtgttta tgatgtgact tcctttaaac tcctttctct gattaaacaa
     3721 cttaatcaag gtttatctta tagtggtgct agcttgagac gtccaacaga ttttacagtt
     3781 gctgctgcct ttaatcctaa tgtgaaaaac cttactcgaa ccgtaaaatt gattgagaaa
     3841 aaagtagctt ctggtgctga ttattttatg acacaaccaa tatttgatca ctctgtgtta
     3901 aaggaattag ctgatcttac taaaacagtt gaacaaccct tttttatcgg aattatgcca
     3961 ataacaagtt ataataacgc tgtttttcta cacaatgaag ttcctggtat taaactttct
     4021 gagtcatttt tatctgctct tgaaaaggta aaggacgaca aagaagcttg tttaactttg
     4081 gccttaaacg agagtaagtc cttgattgat gaagccttga actattttaa tggtatctat
     4141 ttgattactc cctttttacg ctatgatctg actcttgagt taattgacta tatacaaaaa
     4201 aagcaagtaa gaaagtcctc ttaactttct tactttttag ttggtcgttt attattataa
     4261 taaaagttac gtaccaaaac agaggttaaa ctagattttt aagccacctt ctatactaag
     4321 ataaattagt taatttaagt tttctttaag tttatagact tagaataaag cgttattttg
     4381 atgggtagca taaaattaag gcataatatg gatgtcagcg atttttttag actaacgaaa
     4441 taacgctgta aagcttaata ggggtgtatc aaatactcac ttatatagta ccaaaagcct
     4501 atagcctgtt ggctagcttg accgtattca aatattgaat aaatcttggt tgagaaaggg
     4561 aggtataaaa atggtatgtg tcgattttat tgatcaaaaa gtttatagtg aagcagaagg
     4621 cctatttgtt tccgatttag acttaggcat taaaggttta ataagagcac gttatccttt
     4681 tgctctggat agtgacttta tttcgtatgg aaatttaagt cattattgta tgaattatct
     4741 tgacgaaatt gttcagcgag cgaatcagaa aaatgagaag ataaaatata atgtgactac
     4801 cttgatgaaa gaagaagaga agcccttcaa cgttgaagag cgtttgaaca agcaagcaac
     4861 tatcggtcaa cgaattgctg atgatgttgc acgttttggc ggatcttgga cttttattat
     4921 cgtttttgta tccattatgg ctatttggat gttggttaat atcatgaagc cttttggtat
     4981 tcaatttgat ccttacccat ttatcctgtt aaacttagct ttatcaacga ttgctgcaat
     5041 ccaagcacca cttattatga tgagtcaaaa tcgtgctgct gattatgatc gtttgcaagc
     5101 tcgaaatgac tttaatgtca ataaaacctc tgagttagaa attagattat tgcacgaaaa
     5161 gattgatcat atggtacaac aagatcagtt tgaattgtta gagattcaaa agttacagac
     5221 agaaatgcta gtatcattgg gaaatcagtt agcacaattg aaacaactac aaaaataatc
     5281 ttttagcagt tatttaaaaa tactgataca atactaagaa tttaaattaa tactgcaact
     5341 tcctataatg gagagcagta tttttacttt attaataaca aaaacccctt agatgatgtg
     5401 tctaagggat ttttgtatcg gaataatcga gttggtgaat tattaggcta gtgtctgtcc
     5461 gaaagcgtct gctgctaaaa tagcttgagc aaacattttg ggatcgaaag gggtattttt
     5521 gtattcatta gtagtcattg caatttcaac aatttttgga atatcttttt ctgataaaga
     5581 gacgtcagct agagttgttg gcaaacctag tgacttatta aaacgagcta ctttttcaag
     5641 ttcttctagt tcgttgaaat aagcatgtaa aactaacaca ccaaacgcaa ctacaacgcc
     5701 atgtaagaat tcaccttgtc tttgaatagc ggttgtacca tagtaaaatg catgcgcgtg
     5761 acaagaattg tagtagaaat caggttgatt aactaaatta gaagcatagc cagttgagat
     5821 taagatatca agcgcaattt cttcaactgc acgcgaagct aaattagctt caacatcttt
     5881 tagaccttgt tcaccaaatt gataaaaagc ttcctttgaa gacagagcga ctgcttgtcc
     5941 taacactgca gtatgtggta gtttattggt cttagcctct aaggtagcac gttctacttc
     6001 aggggctttt gaaataccgt ccccaatacc agcccaaaaa tatttgcttg gtgcctgtgc
     6061 aataatccgt gtattgataa aaatatgaag tggagactcg ggatagccgt atcttaagaa
     6121 agaatggtca tcattatata caactgcaat ggcagtacct gcggagcaat tagaacaaat
     6181 agttgggata gtaaagctat ttttacctaa ctcttttgct accattttag ctgtgtctaa
     6241 agcttttcca ccgccaaaac ctaagatagc atcagcggct tgaacttgtg ggttggctac
     6301 taacttgtca atgttagatt gtgttgaatc cgttccatag acaaagcttc ctgtgacttt
     6361 aatatttgct tcctctaaaa tagagcgaac ctcggcttct gagctgtcta gggcacgttt
     6421 gccaccgacg aaagcaatgc tttgaatacg gtaagttgcc aaaacgctag gaatttcttt
     6481 gaagcaatct tctccgtagc tataatttgc cattacattt gttgtcattc agtgtcctcc
     6541 tctattccaa aggtcgatta gagacagcta ttttctaaaa gtgaatagtt ttttgtctct
     6601 tgaccaagtg attatcatat tatagcagaa tattcagaaa attggaacat gaaaaacaat
     6661 ttaattttca agaataagaa tagaaaaggt aaagcgagat atacgagtaa atactgtcag
     6721 tataataatt acaagctctt gagagggtct gtattggatg caacagggag ttcttttagg
     6781 tattgatcaa gatcgcgttg gtgttttaga atctttattt tttgagggta gtcctcaaca
     6841 acttgtttat agtttgattt ttgtttgtca gagcgaccgt ctttaagaat ccaactgata
     6901 aattcaaaat ctaatttttc cgggcatcca ttggccatat caggtctagt cttaccacga
     6961 aacttacagt aacgtttgaa tgctctccac aagcagttga agcgattaaa gtcaaaaaaa
     7021 ataatctcat ctgcatctgc tagacgttct tgatatagca gttttttgta gttaccttct
     7081 ataatccatg ttcttttttc taacatgttg ctgacatcgt caatcatgtc atcatatttt
     7141 ctctcttccc aatttggggc gaagtgaata ctatcaagat gtaatacatt gcaattatag
     7201 taatttccca attttcttgc taaagtggac ttacccgacc cactatatcc gattat
//
LOCUS       DATRTN010000082         7147 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.082, whole genome shotgun sequence.
ACCESSION   DATRTN010000082 DATRTN010000000
VERSION     DATRTN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.082"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            296..1411
                     /locus_tag="VBE22_001657"
     CDS             296..1411
                     /locus_tag="VBE22_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO7203092.1"
                     /translation="MSDDLISIQSSCLSIAKTFANFNIQRKRFMISTETEMDTFHKKD
                     DIDVWVGGKSDNPARSFRTRTINELTVIDFEEMFDILWLMLGDNLIKSFEVNVCGILF
                     ELGGNDIPSTFRQENIDPLINKWWYDNVSTEIIPNLIKKLKENPLFNIGFMVNDILER
                     MYKENIPKSYLTSVPLVISQKGRTTYSFSMTGGQQIDGVKFTQIYEDYMKLLSQGKDI
                     AELYQKYSKEELANLGINIYQSNDIERTEERTFDEIISWVSNPYATRPIQERHTIQLE
                     PTRFSLEDKKRIEEAAAQGLSEIDLIDLVDLYDINLDNTSVNRHIVGLLTNNTQVTYY
                     FQEQLNKELLSMAHALDNVQQAFIKLLSEEEIRKFAL"
     gene            1732..2181
                     /locus_tag="VBE22_001658"
     CDS             1732..2181
                     /locus_tag="VBE22_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7203093.1"
                     /translation="MSNKVQERRERKIKEAIKAKNWDEVTRLLQQEQSNAERRDRYHN
                     RRIKDETIASKNAKKSVRYDVIASSDLNPEEALILEELRQAIREAKASLSEIDSKIVE
                     MIAEQGSSYKETARYITEHYKKMSDVTVKSHYCKALKKLAPLLKAYR"
     gene            2383..2703
                     /locus_tag="VBE22_001659"
     CDS             2383..2703
                     /locus_tag="VBE22_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203094.1"
                     /translation="MAFKMKTTKGGYTTTLADKIISQKQPIYSLSTELEPQQRFEEGK
                     PTGEIVAYKAWFVQEGQDPFQVKFEETIELPAFQSMIQFDTLQACEVKYNIYFKANGI
                     KEVR"
     gene            2703..3803
                     /locus_tag="VBE22_001660"
     CDS             2703..3803
                     /locus_tag="VBE22_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO7203095.1"
                     /translation="MTSQSRDVLNQSYLQSYLLQSLNMALGALMQGETSYTNSFNIVI
                     QADGFIFVPRLPCAYILDDDLYKKIFLIANASLYPQYTLLKQNATYFVPLETDDLHTQ
                     RGLFFPWKKGISERLVIPDLDKFSASLPQGKIPIMKYFELNLDKVNHWAIAGNSGSGK
                     SYALTYFLSVLKHMSDLIIIDPKFDTPSRWARENHVAVIHPVENRSKSDFVSQVNEQL
                     SQCATLIQKRQAVLYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQISLLG
                     RATKIHLFLVSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPIG
                     HGTGIIQVIDNEHPYQVLPLLCPTYYTKRGIL"
     gene            3800..4405
                     /locus_tag="VBE22_001661"
     CDS             3800..4405
                     /locus_tag="VBE22_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203096.1"
                     /translation="MTNKTYFMRSFNLILYLFLFAPICSAFGWLFNYLSVQLTNITFV
                     NLDTVKSITDIISSVCHGFALISLLLALFLVGLEIIQRLKTDSLWNYIQSVYHTFLLR
                     HFLFQRERVQKISNLEHQTVTTINPIHNGFNRAVRKCIVDIRKDTITIFLKVPRDQQG
                     QKILKEMEAQLKEEIVSQHADYYFSSPIRVRNQLWFTGQKR"
     gene            4611..5216
                     /locus_tag="VBE22_001662"
     CDS             4611..5216
                     /locus_tag="VBE22_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEO7203097.1"
                     /translation="MAKEQRSTKWTFLFYEESAPENYLNILEELHIPFILSPWHDKDV
                     NRQTGEFKKSHKHGAFFFDSLKSYSQVSNIISDKLNGPAHVEVVMSPTGLFDYFTHAE
                     NPDKTPYNIEDIEVGCGFNLEKFLMEMNSSDFIHEVVDIIEENDFTEFEELVWYARAN
                     NTNLLGLIIERTYFFAKYLDSRRYNPNRLHNSNTEEKENNE"
     gene            5209..5457
                     /locus_tag="VBE22_001663"
     CDS             5209..5457
                     /locus_tag="VBE22_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO7203098.1"
                     /translation="MNNEKLEQIEQLLQTLIKLIQIKEQNIPLKIIQQRELLKQLNIS
                     PNTLKTWEQKGLKRLEPPIEGTRTVFYLLDDIINFLQS"
     gene            5547..6812
                     /locus_tag="VBE22_001664"
     CDS             5547..6812
                     /locus_tag="VBE22_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7203099.1"
                     /translation="METETIIHNGKKVIKKIKKDKSISYTLKGTFLGKDIKTGKQVTT
                     TITAKTLKQLDRAIIQARLEFEKNGSTREKVIVIDTLEDLAEEWFKSYKTWVNSHNTL
                     NRVRGYLDNYIIPKFGDYKPDKIESADIQLWLNDLAKKSKESIESGIKRADKGSAKDF
                     GAVIHKLKDIFDYGITNYGLITNPVSTVKIPPKPKSNKQRIMVLHDDGLVIWLNYLES
                     LDNNRANRRFKLICNTLLASALRINELLALTIDDLDFENSSINVSKTLMWKTANKKDG
                     TKGEVICKATPKTDAGNRSVPVPPPIIEKLRDFHHEMNKYLELHNRHRTKLIFPTIYG
                     NYMCDRNERTTLKKRLVGLGLPNYGFHLFRHTHASMLLNAGTNWKELQVRMGHKSIST
                     TMDTYAELAPQRKLEAVGIYLEKIAELTQ"
BASE COUNT         2542 a         1290 c         1204 g         2111 t
ORIGIN      
        1 attttttgaa gaaaattatt cacaagaaat acctactcgt atcaaaaatt taagaaaaaa
       61 atacaatatt acacaaagcg aactaggcaa tgcaggtcaa gttagtcaag ttgaaagtgg
      121 taagcgacca attacgagtt cgatgttagt ttatttaaat gctctaaccg cttcgagcta
      181 tacgtatatc gtttttggag agttagatga gtttattgag aatctgtttc attatttttt
      241 cagttctatt ttatatagag atttagaggc tgtagatgaa aaactatatt ctttcatgag
      301 tgatgattta atttcaatcc aatcaagttg tctaagtatt gccaagacat ttgcaaattt
      361 caatattcaa agaaaaagat ttatgatttc tactgaaact gaaatggata cttttcacaa
      421 gaaagatgac attgacgtat gggttggtgg aaaaagtgat aaccccgcta gaagttttag
      481 aacccgtacc atcaatgaat tgactgtcat tgattttgaa gaaatgtttg atattctttg
      541 gttgatgtta ggggataatt taattaaatc atttgaagtt aatgtctgtg gtatcctatt
      601 tgaattaggc ggaaatgaca taccctcaac atttaggcaa gaaaacattg atcctcttat
      661 caataaatgg tggtatgata atgtttcgac agaaattatc cctaatctaa taaaaaaact
      721 caaagaaaat cccttgttta atatcggatt tatggtaaat gatatattag aaagaatgta
      781 taaagagaat attccaaaat cttaccttac atccgtccca ttagttattt ctcaaaaagg
      841 aagaacaacc tattcgttta gtatgactgg tggtcaacaa atagatggag tgaaattcac
      901 acagatatat gaggactata tgaaattact cagtcaaggt aaggatatcg cagagttata
      961 tcaaaaatat tctaaagaag agttggcaaa tctaggcatt aatatttatc aatccaatga
     1021 tatagaaagg actgaggaaa gaacttttga tgaaattatc agttgggttt ccaaccctta
     1081 tgcaacaaga ccaattcaag aaaggcacac tattcaatta gagccaacaa gattttcact
     1141 agaggataag aaaagaattg aagaagctgc agctcaagga ctaagcgaaa tcgaccttat
     1201 tgatttagtt gacctatatg atattaattt agacaataca agcgtcaatc gccatattgt
     1261 ggggttattg actaataaca cccaagtaac atactatttc caagaacaat taaataagga
     1321 gttgctgtca atggctcacg ctttagataa cgtacaacag gcctttatta aattattaag
     1381 tgaagaggag atacgaaaat ttgctcttta aagactaggt gaatcgccta gtcttttttt
     1441 gcgcctcgtt ttacaaatta gtctattgaa gtaaaaaaag aagtaaaaaa taaaagaaca
     1501 ttacgcctta tactgaatat atcaagaaaa actcttgata taaattttaa tagtccaaat
     1561 tgatggccat caaaggacag attgaaacaa atatgatgac atgacgataa aggtcatgtc
     1621 ttgcatagaa ttgtttcgct gtttctttgt tgtgcctttt ggtatactca gcgttcaata
     1681 tttgctcatt ttgtttcctt ctgtccaaag gacagaaagg agctcatcat tatgagtaat
     1741 aaagtacaag aacgtcgtga gcgtaaaatc aaagaagcta ttaaagctaa aaattgggat
     1801 gaagtaactc gtttgcttca acaagaacaa agtaacgcag aacgtcgtga ccgataccac
     1861 aatagacgaa tcaaagacga aacaatagct agcaaaaatg ccaaaaagtc tgtacgttat
     1921 gatgtcattg caagttctga tttgaatccc gaagaagctt taatcctaga ggagttaaga
     1981 caagctattc gtgaagctaa agcatcccta tcagaaattg acagtaagat tgttgaaatg
     2041 atagcggaac aaggttcaag ctataaagaa acggctcgct atatcactga acattataag
     2101 aaaatgagtg atgtaactgt taaatcccat tattgtaaag cgctaaaaaa attagctcct
     2161 ttattaaaag cttaccgcta atccctcaaa tccagtgtct ctgtcactgg attttttgta
     2221 aaaaaaaatt aaaaattcaa caaataactt tgcgtttagc tgtctcattc tccttagtaa
     2281 tgaggaggtg gtcagataac ctaaaaaaca aagtccagct catgacccaa accgagcctg
     2341 tcgcattcac actatctctt attcataagg aggatttgcc ctatggcgtt taaaatgaaa
     2401 accacaaaag gtggttatac aactacattg gctgataaaa ttatcagtca aaaacaaccg
     2461 atttactcac ttagtaccga acttgaacca caacaacggt ttgaagaagg aaaaccaacc
     2521 ggagaaatcg tagcttataa agcatggttt gtacaggaag ggcaagaccc tttccaagta
     2581 aaatttgaag aaacgattga gcttccagct tttcaatcca tgattcaatt tgacacacta
     2641 caagcctgcg aagtaaaata taacatctac ttcaaagcaa atggtatcaa ggaggttcgg
     2701 taatgacttc acaatctcgt gatgtgctga accaatctta cctgcaatct tacctgctcc
     2761 agagcctaaa catggctctg ggggctttaa tgcagggaga aaccagctac accaattctt
     2821 ttaatattgt tattcaagca gacggcttta tctttgtccc tcgtctacca tgtgcttata
     2881 tccttgatga tgacttgtac aagaaaatct ttctgattgc caatgcttca ctttatccac
     2941 aatatacgct tctaaaacaa aacgctacct attttgtccc tctagaaaca gatgacttac
     3001 acactcagcg tggattattt ttcccttgga aaaagggaat ttcagaacgt ttagtcattc
     3061 cagatttgga taagttttca gctagcttgc cacaggggaa aatccctatc atgaagtact
     3121 ttgaactcaa tttagataag gttaatcact gggctattgc tggaaattca ggttctggga
     3181 aatcgtacgc tctcacttat ttcttgagtg tactaaagca tatgtctgac ttgattatca
     3241 ttgaccctaa atttgatacc ccaagtcgat gggcaagaga aaatcacgtt gctgttatac
     3301 accctgttga aaaccgttca aaatcagatt ttgtctcaca ggtcaatgaa caattaagtc
     3361 agtgtgcaac tctcattcag aaacgacaag ctgtcttgta tgataatccc aatcatcaat
     3421 ttacccatct aactattgtt attgatgaag tcctcgctct atcagaggga gtcaataaga
     3481 atatcaaaga agcctttttc tccctactct cacagatttc cttattagga cgtgctacca
     3541 aaatccatct gtttttggta agccagcgtt ttgaccacaa tactattcca atttcagtga
     3601 gggaacagct aaacgtattg ttgcaaattg gaaatatcaa tcagaaaact actcaatttt
     3661 tatttccaga cctagaccca gagggtattg ttattccaat aggacatggg acaggtatca
     3721 ttcaagttat cgataatgaa catccctacc aagttctgcc cttactctgt ccaacctact
     3781 acactaaacg aggtattcta tgaccaataa aacctacttt atgagaagtt ttaatctcat
     3841 actatattta tttctatttg cacctatctg ttctgcattt ggatggttat tcaattatct
     3901 ttccgttcaa ttaacaaata tcacgtttgt caatttagat acggtcaaaa gtattacgga
     3961 tatcattagt tccgtttgtc atggatttgc acttatttct cttctacttg ccttattttt
     4021 ggttggatta gaaataatac aacggttgaa aacagatagt ctttggaact atatccaatc
     4081 tgtctatcac acttttctgt tgcgacactt cctgtttcag cgtgaacgag tacagaagat
     4141 aagcaatctt gaacatcaga cggttactac tatcaaccca atccataacg gatttaaccg
     4201 agctgttcgt aagtgtatcg tagatatacg aaaagatacc atcactattt ttctaaaagt
     4261 ccctagagac caacaagggc aaaaaatcct aaaagagatg gaagcacaat taaaagaaga
     4321 aatcgtcagc caacacgcag actattattt ctcctctcct attcgagttc gtaaccaatt
     4381 gtggttcact ggacaaaaac gttaatccct tggggctgtg gcttgcgtta agcaaaagcc
     4441 agacagcccc tttttatatt cttttctatc atgacctatt ggggtgacta cgacccccaa
     4501 taggtcaata atcaataatc acaaaaaatt caattttttc aaaaaaactt tgcatacgac
     4561 ctgtcatttc tccttagtaa tagaacccaa aagaaatgag gtaactgcct atggcaaaag
     4621 aacagcgctc aaccaaatgg acttttctct tctacgagga aagcgcccca gagaactact
     4681 tgaatatatt agaggaactc catattccgt ttattcttag tccttggcac gataaggacg
     4741 ttaatagaca gacgggagag ttcaagaaat cgcacaaaca cggtgctttc ttctttgatt
     4801 cactgaaaag ctattcacaa gtatcaaata tcatcagtga caaactaaac ggtccagcac
     4861 atgtggaggt agttatgtct cccactggtt tatttgacta ctttacccat gcggaaaatc
     4921 cagataagac cccctacaac attgaggata ttgaagtcgg ttgcggcttt aatttagaaa
     4981 aattcttgat ggagatgaac tcctcggatt ttatacatga agtagttgat attatcgaag
     5041 agaacgactt taccgaattt gaagaattgg tctggtatgc ccgagcaaac aataccaatt
     5101 tattagggct tatcattgaa cgtacatatt tcttcgctaa atacctagat tcacgaagat
     5161 acaatcctaa tcgactccat aattcaaaca cagaggagaa agagaataat gaataacgaa
     5221 aaattagaac aaattgaaca attattgcaa acacttatca aactcattca aatcaaagaa
     5281 cagaacatac cactaaagat aatacaacag cgagaattgc taaaacagct aaatatctcc
     5341 ccaaacacac taaaaacttg ggaacaaaaa ggattaaaaa gattagaacc tcctatagag
     5401 ggaactcgaa ccgtcttcta tctactagat gatattatca actttttaca atcctaattc
     5461 tgaaaatttg tgctaaaata gttttatcta aaagaaagag tggtaactat tttagcctac
     5521 ttcaatatcc atgaagaaag gcaaatatgg aaactgaaac aattattcat aatggcaaaa
     5581 aagtcattaa aaaaatcaaa aaagataagt ccatctcata cactttgaag gggacatttc
     5641 tgggaaaaga tatcaaaact ggtaagcaag tcactactac cattacagcc aagacactaa
     5701 aacagttaga tagagcaatc attcaagcac gactagaatt tgaaaaaaat ggctcaacac
     5761 gagaaaaagt tatcgtcatt gatacactgg aagatttagc tgaagaatgg tttaaatcat
     5821 ataaaacctg ggttaactcg cataatactc tgaacagagt aagaggttat cttgataatt
     5881 atattatccc taaatttgga gattacaaac cagataaaat tgaatctgcg gatatccagc
     5941 tttggttaaa cgatttggca aagaaatcaa aagagtccat tgaatctggt ataaagcgtg
     6001 ctgataaagg tagtgctaaa gattttggtg ctgttatcca taagttgaaa gatatttttg
     6061 actatggaat tacaaactat ggactaatca ctaatcctgt tagcactgtt aagataccac
     6121 ctaagccaaa atcaaataag caacgcatca tggtattaca tgatgatggt cttgtaatat
     6181 ggcttaatta tcttgaaagt ttagataaca atagagccaa tcgtagattt aagttaattt
     6241 gtaatactct attagcctcc gctttgcgaa taaatgaatt acttgcattg acgattgatg
     6301 acctagattt tgaaaattct tctattaacg tcagtaaaac attgatgtgg aaaaccgcaa
     6361 ataaaaaaga tgggacaaaa ggggaagtta tctgtaaagc tacaccaaaa actgatgcag
     6421 gaaatcgctc tgtccctgtc cctcccccca taatagaaaa actcagagat ttccaccacg
     6481 aaatgaacaa gtatcttgaa ttgcacaaca gacatagaac aaaactgatt ttccctacaa
     6541 tctatgggaa ctatatgtgt gacagaaacg agagaacaac tcttaagaaa aggttagtag
     6601 gtcttggatt accaaactat ggtttccatt tattccgaca tactcatgct tcaatgctac
     6661 ttaatgctgg aacaaattgg aaagaactcc aagtcagaat gggacataaa tccatttcaa
     6721 ctacaatgga tacttacgct gaattagcac cacaacgaaa gttagaagct gtggggattt
     6781 atcttgaaaa gattgctgag ctaacgcaat aactactcta ccattcactc taccttttat
     6841 tatgtagcat tctagaaaca ttgatgtacc aacgtttcta acagctaaaa tagaaattat
     6901 ttaactctta ctgaagttaa ataattgatt ataaaaaagc ctatgaaatc aatatttctg
     6961 ggctttttat ttttaaaaat aaaatctgac aaaaaagtat aaatttacct cttaccttca
     7021 ggtagttagt cattctttta aaactcactt ttttataact gaaaatattg ttcttaattt
     7081 taaaaattca ttttacgact atatactcag aagtaacaaa cctaatccaa ggtttgaaaa
     7141 aagaaag
//
LOCUS       DATRTN010000083         7143 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.083, whole genome shotgun sequence.
ACCESSION   DATRTN010000083 DATRTN010000000
VERSION     DATRTN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.083"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..1075
                     /locus_tag="VBE22_001665"
     CDS             <1..1075
                     /locus_tag="VBE22_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7203100.1"
                     /translation="MDCTISNTKIFNEEIKHFKFQKPMPYPTYFRYFIPEVIHEDKVL
                     YLDCDMIITSDLTSIFTLDISKYGVAAVRDDLLEEYDGKEDYFNSGLLLINNIFWREQ
                     GISQRLLDYTRENQGALQYHDQDVLNDVLCDNWLELDETYNYHTGADMLYNLFQQSER
                     QLNRRKDLPKVIHYTATKPWKYLETSVRWRDIWWEYNRLEWRDIFTRWQVNDGVDVSL
                     KKVLQPIHRAFIFTYSDSIEGIERLAKDLPNWQFHIAAYTQISEKLKQLEMHSNIKLY
                     PLVYRFKLNELVENSDIYLDINHGDADTQVLEQVKKLGKDILSLSTTYHGQGKQFERV
                     TDIIDYLKNERFKEHIRKEYCEK"
     gene            1065..2249
                     /locus_tag="VBE22_001666"
     CDS             1065..2249
                     /locus_tag="VBE22_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEO7203101.1"
                     /translation="MKNNVICLAGDNKSLNQIQTVIKSILCHNDRVSIYILNQDIASE
                     WFRNIQRRLLNSHSCIFDIKLFDDTFKEFKTPRAHITYMAYARYYIPQLIDAEKVLYL
                     DIDTLVVDNLDKLFEIELGDYPIAAILDGDGIHFNSGVMLINSLYWMRYRVTEKLLEI
                     TERELDNGIFGDQGVLNLLFDNNWLKLEDKYNAQVGNDLGAFYENWQGYFDRNFESPT
                     IIHYCTHDKPWNTFSSSRFRETWWQYEQLDWNEVFNFETYLLPEPTFEKHFFTFTTSV
                     DLLYIEELVELFPNSCFHIAAWTSFGPRLLKLATNSNVRLYPSITSPLFEQLMTKANY
                     YLDISTSWKEIQFCQKAIEKSIPILSFNEAVTFEYRELSHCFINLEDMRQFLCKNGGN
                     SD"
     gene            2242..3117
                     /locus_tag="VBE22_001667"
     CDS             2242..3117
                     /locus_tag="VBE22_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7203102.1"
                     /translation="MTDKLVSVIIPVYNAAPYLEGCVNTILGQTYQVFEILLIDDGST
                     DTSASICDQLSLRDNRIRVFHIENGGASKARNFGLARISPESQFVTFVDSDDWVKENY
                     LEVLLAQQEKYNADIVISNYYIYRETEDIFGYYITDKDFVIEEISAQTAIDRQVHWHL
                     NSSVFIVIWGKLYRRELFDTITFPIDKVFEDELVSVLLFIKSKKTILVNGSYYGYRIR
                     PNSIMTSAFSSKRAEDLIYIFDSKLSEIILSGLDSTATVERYFWLMRDYKQRLEQENL
                     QKTIIYKVISNRLSR"
     gene            3178..4374
                     /locus_tag="VBE22_001668"
     CDS             3178..4374
                     /locus_tag="VBE22_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEO7203103.1"
                     /translation="MRVIKVKTISETLDYISKHTASVARFGDGEIDIIAGNSIPYQVY
                     DSELAKRLERLLNRQSNEKLVICVSDVFDNLGRYNDFSQQFWRDHLYQYNDLYQKNCQ
                     AEWYGSTFISRPYMDLLDKSLSVTYFEQLKALWKNKDILIVEGETSRSGVGNDLFNDA
                     NTISRIICPSKDAFSSFYEIKETILEHSRGKLILLMLGPTAKLLAEDLSQLGHQAIDL
                     GHIDSEYEWFKMGAVSKVKLSHKHTAEHNFDEDITLIEDNSYENQIVSKIGTSDRQYA
                     LTVTLTDDIWELEHLLQRCPNTDFHIAAPVYCSDRLKQLVGYPNYYLHEAITEEQFEV
                     LLLNSDIYLDINHGEEVWNVVDRCITEGKTIYGIRRARKDNQYISFERTMDDFEYLCD
                     TIKQNR"
     gene            4498..5726
                     /gene="secY2"
                     /locus_tag="VBE22_001669"
                     /pseudo
     CDS             4498..5726
                     /gene="secY2"
                     /locus_tag="VBE22_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
     gene            5726..>7143
                     /gene="asp1"
                     /locus_tag="VBE22_001670"
     CDS             5726..>7143
                     /gene="asp1"
                     /locus_tag="VBE22_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO7203104.1"
                     /translation="MFHFIPSWYNENRTWYDNNYLWYFKPTNVGFDDTINQMKMFDYA
                     GKESRLVVLNYMPNLRYYLHRYDLLESGYYSVFDDIQEIGNVRQQMIDFRQLNWPEGV
                     DFTYTPFIVLVKKSGDLIAKVQFGEEGNLTHIDYFANEQIAKKYLFDDRGFLSSILYY
                     DNGGEAYQDYLAPSGERIMREYLREGDHHVEINPKKAIHFLKLSYSDIEELIREKYLT
                     YLHKEVSKSDTIIVSFNQVHNAFIVGNTSKGNLILSVFSERNNAHNVLEDYSSLSRAD
                     AIICDRLDIAAQLKEKIDKPVVHVSPFDTRLALGKSNQVRDLEIYFVVDRLSHKELQK
                     SLTSLYKVMLKNNDIKVTFVSYEREFESRQLTYDYLKEATKVFDQKFFSLSDDDANQV
                     LMQSDEVEKEKTRLSFTHPLSETDIINRLEYVRLIIDISKIPDLYTQIAGISSGIPQI
                     NTILTEFVEHRKNGYVIEEIQE"
BASE COUNT         2393 a          998 c         1317 g         2435 t
ORIGIN      
        1 aatggattgc acaatatcta acactaaaat ttttaatgaa gaaattaaac attttaaatt
       61 tcaaaaaccg atgccctacc ctacttactt tcgatacttt atacctgaag ttattcatga
      121 agataaggta ctatatttag actgtgatat gattatcacc agtgatttaa catcgatatt
      181 tactcttgat atttcaaaat atggtgtagc ggctgttcga gatgatttgc tcgaagaata
      241 tgatggtaaa gaagattatt ttaattcagg tttgctatta attaataata ttttttggcg
      301 agaacaaggg attagtcagc gactgttaga ttatactaga gaaaaccaag gggctcttca
      361 atatcatgac caggatgttt tgaatgatgt actgtgcgat aattggctag aacttgatga
      421 aacttataac tatcatacag gagcagatat gttatataac ctattccaac agtctgaacg
      481 tcaattaaat cgcaggaaag atttacctaa agttattcat tatacagcga caaaaccatg
      541 gaaatacctt gaaacatctg ttcgttggcg cgatatatgg tgggaatata atagacttga
      601 atggcgcgat atttttacaa gatggcaagt caatgatggt gtagatgttt cattgaagaa
      661 agtgttacaa cctatccatc gagctttcat ttttacttac tcagattcaa ttgaaggaat
      721 tgagaggttg gctaaggatt taccaaactg gcaatttcat attgctgcct atactcaaat
      781 ttcagaaaaa ttgaaacaat tggaaatgca ttcaaatata aaactttatc cacttgttta
      841 tcgtttcaaa ttaaatgagc tagttgaaaa ttcagatatt taccttgata ttaatcatgg
      901 tgatgcagat actcaggttc tggagcaagt gaagaaatta ggtaaagata ttttatccct
      961 ttctacaact tatcatggtc aaggcaaaca atttgagaga gttacggata ttattgatta
     1021 tttaaagaat gaacgattta aagaacatat tagaaaggaa tattgtgaaa aataacgtta
     1081 tttgtttggc aggtgataat aaatctttaa atcagatcca aacagttata aaatcgattt
     1141 tatgtcacaa tgatagagta agtatttata ttttaaatca agatatagct tcagaatggt
     1201 ttagaaatat tcaaagacgt ttattaaata gtcatagttg tatttttgat ataaaactct
     1261 ttgatgatac ttttaaggaa tttaaaacgc cccgtgccca tattacctat atggcttatg
     1321 caaggtacta cattccgcaa ctgattgatg cagagaaagt tctttattta gatattgata
     1381 ctttggttgt tgataactta gataagttgt ttgagataga attaggggac tacccgatag
     1441 cagctatctt agatggagac ggtattcatt ttaactcagg tgttatgcta ataaactcgc
     1501 tttactggat gagatatcga gtaacagaaa aacttctgga aataacagaa cgagaattgg
     1561 ataatggaat ttttggagac caaggggttc tcaatcttct ttttgataat aattggctta
     1621 agttagaaga taagtataat gctcaagttg gaaatgattt aggtgccttt tatgaaaatt
     1681 ggcaagggta ttttgatcgt aactttgaat cgcctactat tatccattac tgtactcatg
     1741 ataaaccatg gaatactttt tcttcttcaa ggtttaggga gacatggtgg caatatgagc
     1801 aactagattg gaatgaggtg tttaattttg aaacgtatct gttacctgag ccaacatttg
     1861 aaaaacactt ctttactttc acaacgtccg tggatttgct ttatattgaa gaattggttg
     1921 aactattccc caatagttgc ttccatatag ctgcttggac ctcttttggc ccaagattac
     1981 ttaagttagc cacaaactct aacgttcgtc tgtatccttc aataaccagc ccactttttg
     2041 aacaactaat gactaaagca aattattacc ttgatatctc aacttcttgg aaggaaatac
     2101 aattttgtca aaaagctatt gagaaatcaa ttccgatatt gagttttaat gaagcagtga
     2161 catttgaata tagggagtta agtcattgct ttattaattt ggaggatatg cgacagtttt
     2221 tatgtaaaaa tggaggcaat agtgactgat aaattagttt ctgttattat tccagtctat
     2281 aatgcagcac catatttaga ggggtgtgta aataccattt taggacaaac ataccaagtt
     2341 tttgaaattc tactgataga tgacggttct acagatacat ctgctagtat ctgcgatcaa
     2401 ttatccctaa gagataaccg tattagagtt ttccatatcg aaaatggcgg tgcttctaaa
     2461 gctagaaact ttggattagc acgaattagt ccagagagcc aatttgttac gtttgttgat
     2521 tcggacgatt gggtaaagga aaattatttg gaagtactgc tagctcagca agaaaaatat
     2581 aatgctgata ttgttatcag taattactat atctacagag aaacagaaga tatttttggt
     2641 tactacatca ctgacaaaga ttttgttatc gaagaaatct ctgctcaaac agctattgat
     2701 agacaagtgc attggcattt aaatagttca gtattcatcg ttatttgggg gaaattatat
     2761 cgaagagaac tatttgatac catcaccttc ccaatagata aagtatttga agatgagttg
     2821 gtaagtgttt tactttttat aaaaagtaaa aaaacaattt tagttaatgg aagctattat
     2881 ggctatagaa ttcgtccaaa tagcattatg acatcggcgt tttcatccaa aagagcagag
     2941 gatcttattt atatttttga tagtaaattg tcggagatta tactatcagg attggattca
     3001 actgcaactg tagagagata tttttggtta atgcgtgact ataaacagcg cttagaacaa
     3061 gaaaatttac agaaaacaat aatatataaa gtgatatcaa atcggttatc aaggtaacag
     3121 taaataccat tttatttgtc gtaatcttgc aaagagagtt gaaaagaatt aatagttatg
     3181 agagttatta aagtaaaaac aatttcggaa actttagatt atatttctaa acataccgct
     3241 tcggtagcgc gttttggtga tggagaaatt gatattattg cggggaattc tattccttac
     3301 caggtttatg attctgaact tgctaagcga ttagaacgtt tattaaatcg acagagtaat
     3361 gagaagttag ttatctgtgt aagtgatgtt tttgataatt taggtagata caacgatttt
     3421 tcacaacaat tttggaggga tcatctatat cagtataatg atttgtacca aaaaaattgt
     3481 caggctgaat ggtatggttc aacttttatt tctagaccct atatggattt actggataag
     3541 agtttgtcag taacgtactt tgaacagcta aaagcattat ggaaaaataa agatatttta
     3601 attgtcgagg gtgagacttc tcgttcaggc gttggcaatg acttatttaa tgatgccaat
     3661 actatttcgc gtatcatttg tccttcgaaa gatgcatttt catcttttta tgaaatcaag
     3721 gaaactatac ttgaacatag tagaggtaag ctaattttac ttatgcttgg tccaactgca
     3781 aagctattag ctgaagattt atcgcagcta ggacatcaag ctattgattt aggtcatatc
     3841 gattcagaat atgagtggtt taagatggga gcagtttcta aggtgaaact ttctcataaa
     3901 catacggcag aacataattt tgacgaagat attactttaa tagaagacaa tagttacgaa
     3961 aatcaaattg tatcaaaaat tggtacatca gatagacagt acgctttgac agtgacgtta
     4021 acagatgata tatgggagct agaacattta ttacaaagat gtcctaatac tgattttcat
     4081 attgctgccc cagtttattg ttcggataga cttaagcagt tagtaggtta tccaaattat
     4141 tacctacatg aagctattac tgaagagcaa ttcgaagtac tcctattaaa tagcgatata
     4201 tatttagata ttaatcatgg tgaagaagtt tggaatgttg ttgatagatg tataactgag
     4261 ggtaaaacta tatatggaat cagacgggca agaaaagata atcaatatat ttcttttgag
     4321 cgaaccatgg atgattttga atatttgtgt gacactatta aacaaaatag atagtcaaga
     4381 agagtaatgg ttacttgaat attgaaatca attttcttga ttttaaaatt aacgaagttg
     4441 acgatataaa gttacttatc ttgaagagaa caaattgata gagaaagaga aattccattg
     4501 aaattactat atatttttga aaaaaatatt attcttcgta aaatattgat aacattttca
     4561 ttaataatca ttttttactt ggtcgctatg ttccaatacc tggtgtcttg atttctgctt
     4621 ataagggaca agacaataac tttgcgacgc tttattcaac agtaactggt ggtaatttgt
     4681 ctcaggttgg ggtcttttca ttgggtattg gtcctatgat gaccacaatg attttactaa
     4741 gactatttac gattggtaaa tactctagtg gagtatctca aaaagttcaa cagtttcgcc
     4801 aaaatgtggt gatgcttgtc atagcaatta ttcaagggct agccatagcc atatcttttc
     4861 aatatcataa tggtttttct ctgaccaaat tattactcgc gacaatgata cttgtgactg
     4921 gtgcatatat tatttcctgg attggtaatc taaatgcgga atacgggttt ggtggaatga
     4981 ctattttagt ggtggtaggt atgcttgttg ggcaatttaa taatattcct cttatttttg
     5041 aattattcca agatggttat caacttgcta tcatcttatt tcttttgtgg acactagtag
     5101 caatgtactt aatgataact tttgaacgtt cagaatatcg tataccagta atgcgtactt
     5161 caatccataa tcgtctcgtt gatgatgcct acatgccaat aaaagtaaat gcttcggggg
     5221 ggatggcatt tatgtatgtt tataccttac taatgttccc ccagtatatt attattttac
     5281 taagaagtat tttcccaact aatccagata tcacatcata caatgactat tttagtttat
     5341 cttctattca gggagtggtt atttatatga ttttaatgct tgttttatca gttgcattta
     5401 cttttgtgaa tatcgatccg actaaaattt ctgaggcaat gcgagaaagt ggtgatttta
     5461 ttcctaatta tagacctggt aaagaaacac aaagttattt atcgaaaatc tgttatttat
     5521 ttggaacttt tagtggtttt ttcatggctt tcttaggagg ggtaccatta ttatttgctc
     5581 ttggaaatga tgatcttagg acagtttcat ctatgacagg gatttttatg atgatcacag
     5641 ggatgtcatt tatgatattg gatgaattcc aagttattcg tattcgtaaa caatacactt
     5701 ctgtatttga aaatgaggag aattaatgtt tcattttatt ccatcttggt ataatgaaaa
     5761 tcgtacttgg tatgataata attacttgtg gtatttcaag ccgactaatg tcggttttga
     5821 tgatactatc aaccaaatga agatgtttga ttatgctgga aaagagagta gattagttgt
     5881 cttaaactat atgcccaatt tacgttatta tttacatcgc tatgacttgt tagaatcagg
     5941 ttattattca gtttttgatg atattcaaga aattgggaat gtaagacagc aaatgattga
     6001 cttccgacaa ctcaattggc ctgaaggggt ggactttacc tatactccgt ttatagtttt
     6061 ggtaaaaaag tcaggagatt taattgctaa ggttcaattt ggtgaagaag gaaatttaac
     6121 gcatattgat tattttgcaa atgaacaaat agctaagaaa tacctctttg atgatagagg
     6181 atttctatct agtattctct attatgataa tggtggtgaa gcctatcaag attatttagc
     6241 accatcaggg gaaaggatca tgagagaata cctaagagaa ggtgaccacc atgttgaaat
     6301 taatcctaag aaagctattc attttttaaa gttatcttac tccgatattg aagagttgat
     6361 tagagagaaa tatctgacct atctccataa agaagttagt aaatcagata ctatcattgt
     6421 tagctttaat caagttcata acgcatttat tgttgggaac actagtaaag gaaatttaat
     6481 attatctgtt ttctcggaga ggaataatgc tcacaatgta ttggaggatt actcttcctt
     6541 atctcgagca gacgctatta tttgtgatag actagatatt gctgcacaat tgaaagaaaa
     6601 aatagataaa ccagttgttc atgtttctcc ctttgatacg cgtttagctt tgggaaaaag
     6661 taatcaagtt agagatttag aaatatattt tgtcgtagat agactttccc ataaagaatt
     6721 gcaaaagtct ttaacatcct tatataaggt tatgctcaaa aataatgata taaaagtaac
     6781 atttgtcagc tacgaacgag aatttgaaag tcggcaattg acatatgact accttaaaga
     6841 agcaacaaaa gtttttgatc agaaattctt tagtttatca gacgatgatg ccaatcaagt
     6901 tcttatgcaa tctgatgaag tagaaaagga aaaaacacgc ctttctttta cccatccttt
     6961 gtcagaaaca gatatcataa accggttaga gtatgtccgt ttgattattg atatctcgaa
     7021 aataccagac ttatacacgc aaattgcagg gataagttca ggtatccctc agataaatac
     7081 aatattaaca gaatttgttg aacacaggaa aaacggttac gttattgaag agatccaaga
     7141 att
//
LOCUS       DATRTN010000084         7051 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.084, whole genome shotgun sequence.
ACCESSION   DATRTN010000084 DATRTN010000000
VERSION     DATRTN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.084"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            150..569
                     /locus_tag="VBE22_001671"
     CDS             150..569
                     /locus_tag="VBE22_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO7203105.1"
                     /translation="MDNCIFCKIISGEISSSKVYEDDEVLAFLDITQTTTGHTLLIPK
                     KHVRNVLEMDEKTAQITFERLPKVARAVQAATKAKGMNIINNNEEIAGQTVFHAHVHL
                     VPRFDESDGIKIHYTTHEPDFEALAKLAKEIRKEIQL"
     gene            566..862
                     /locus_tag="VBE22_001672"
     CDS             566..862
                     /locus_tag="VBE22_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203106.1"
                     /translation="MKIGRLIALGLVSLGALELYKNRKTIKDSYQNTKNETDSAKLKL
                     ERIKNDLAIISQEKEKIRLISQELNHKFQVFNKDIQPRLEEINQRMAKYQEKDE"
     gene            complement(926..2233)
                     /locus_tag="VBE22_001673"
     CDS             complement(926..2233)
                     /locus_tag="VBE22_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO7203107.1"
                     /translation="MKIWKKITLMFSAIILTTVIALGVYVASAYNFSTNELSKTFKDF
                     KLAKSKSHAIEETKPFSILLMGVDTGSEHRKSKWSGNSDSMILVTINPKTNKTTMTSL
                     ERDVLIKLSGPKNNGQTGVEAKLNAAYASGGAEMALMTVQDLLDINVDYFMQINMQGL
                     VDLVNAVGGITVTNKFDFPISIAANEPEYKAVVEPGTHKINGEQALVYSRMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALNSISSYKKILSAVSNNMQTNIEISSKTIPNLLAYKD
                     SLEHIKSYQLKGEDATLSDGGSYQILTKKHLLAVQNRIKKELDKKRSKTLKTSAILYE
                     DYYGTTASNDSSTYSSTQENNYNTTPYSEAPPSYSGNTTYSSETNQTTHQSYYNSSTP
                     ASNYSSNTNTGQADSSGSVNNYNGAATPNPNTGTQPVPSQTNP"
     gene            complement(2242..2802)
                     /locus_tag="VBE22_001674"
     CDS             complement(2242..2802)
                     /locus_tag="VBE22_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7203108.1"
                     /translation="MGIAQEKLLRRFKMSKQFAVDFIEASREEASALLEFLNTVTEET
                     DFILHTVSNQLSLPEMETFIENTLITKNCICLIAKLKNKVIGLITIISQSDIEIEHIG
                     DLFIAVQKDYWGYGIGHILMEEAIEWASDNDITRRLELSVQGRNERAIHLYQKFSFEI
                     DGLQTRGIKRENGEFLDIYRMSKLID"
     gene            complement(2756..3265)
                     /gene="tsaE"
                     /locus_tag="VBE22_001675"
     CDS             complement(2756..3265)
                     /gene="tsaE"
                     /locus_tag="VBE22_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEO7203109.1"
                     /translation="MLWGCQNQQTVGRQRYFWYNISMFYTQNEEELIALGQKLGTVLK
                     SGDIVLLTGNLGAGKTTLTKGIAKGLDIKQMIKSPTYTIVREYEGRVPLYHLDVYRIG
                     DDPDSIDLDDFLFGQGVTVIEWGELLSDNLINNYLEIVITRSNQGRQVQLEAYGHRAR
                     EIIEAIQDV"
     gene            complement(3348..4769)
                     /locus_tag="VBE22_001676"
     CDS             complement(3348..4769)
                     /locus_tag="VBE22_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEO7203110.1"
                     /translation="MEKFFKLKEHGTTIRTEITAGLTTFFAMSYILFVNPAILSQTGM
                     PAQGVFLATIIGAVVATSVMAFYANLPYAQAPGMGLNAFFTYTVVFALGYTWQEALAM
                     VFICGLISLIITLTKVRKMIIESIPTTLKSAITAGIGTFLAYVGIKNAGFLKFSIDPG
                     TYDVVGKGAAKGLATITANSSATPGLVSFDNPAILLSLIGLSITIFFIVKGIRGGIIL
                     SILTTTLLGILMGVVKLDAINWEATNLSASFRDLKQVFGVALGEKGLISLFSNPSRLP
                     SVLMAILAFSLTDIFDTIGTLIGTGEKVGILATTGDNHESKSLDKALYSDLIGTTFGA
                     ICGTSNVTTYVESAAGIGAGGRTGLTALVVAGLFAISSFFSPLVSIVPSQATAPILVI
                     VGIMMLSNLKDIKWDDMSEAIPAFFTSLFMGFTYSITYGIAAGFLTYTLAKVIKGQAK
                     DIHVVLWILDILFILNFISLAIL"
     gene            complement(4872..5483)
                     /locus_tag="VBE22_001677"
     CDS             complement(4872..5483)
                     /locus_tag="VBE22_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEO7203111.1"
                     /translation="MKVKLFAIHLFFIVISFGIKYYHLQGPNLIWNMTLALIALDFAY
                     LTSLFKKKILIGLFALAWFFFYPNTFYMLTDIIHMHFVGDVLYNKTNLILYILYVSSI
                     LFGFLSGIESFSVIMRKFRISNIFLRWGIIGIVSFVSSFGIHIGRYARLNSWDILTKP
                     QVVINELLAVPSRDSFHFILGFTFLQVLCLIFMDYSAGKKLEK"
     gene            complement(5573..6157)
                     /locus_tag="VBE22_001678"
     CDS             complement(5573..6157)
                     /locus_tag="VBE22_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203112.1"
                     /translation="MKKLIWSHLFAIVSILGIWYYHVKIDHLMVILLASVLSLEMGFI
                     AYYSRNIFIRLLASLGLLFFYPQSLGIFLSLVSLEWSTKDIWQSNHFMQYMAYLLAIL
                     FIISSALLSLKLLVNAYDISKRYQLATVIPVMLFSSFAFCLEKSGSLGLEGIILNPSA
                     FFNGIQTNMANLDFKALSCLFLVQLLLWGILMED"
     gene            6246..>7051
                     /locus_tag="VBE22_001679"
     CDS             6246..>7051
                     /locus_tag="VBE22_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO7203113.1"
                     /translation="MTKKIIAVDLDGTLLHNNNTISDYTADTLRKVQAQGHKVIITTG
                     RPYRMALAHYLRLDLKTPMINFNGALTHIPEKKWAFEHSATIDKKLLLETLNLSDAIQ
                     ADFIASEYRKNFYITMDNRDKINPQLFGVNEITDKMALDVTKITRNPNALLMQTRHKD
                     KYELAKELRQHFNHELEVDSWGGPLNILEFSPKGVNKAYALKHLLKSLNLSQENLIAF
                     GDEHNDTEMLAFAHTGYAMKNASPTLLPYADQQIQWTNEEDGVAKTLEKL"
BASE COUNT         2488 a         1330 c         1032 g         2201 t
ORIGIN      
        1 gctaagataa gtatgatggt aaatatagat atcacaatca atacaggcta cttttatcca
       61 cctgtacgac ttatgaccta tcaaacgata aaaatttacc attccttaat agctacccaa
      121 taaatttttc aacatgaaag gaattagtta tggataattg tattttttgt aaaatcatca
      181 gtggagaaat ctcttcatca aaagtttatg aagatgatga agtattagct tttctcgaca
      241 ttacacaaac tacgactggg cacacccttc ttattcctaa aaaacatgtt cgaaatgtgt
      301 tagagatgga tgaaaaaact gcacagataa cctttgaacg cttgcctaaa gtggcgcgtg
      361 ctgtccaagc tgctacaaaa gccaaaggaa tgaatattat taacaacaat gaggaaattg
      421 ctggacaaac tgtttttcat gcacacgtcc accttgttcc ccgctttgat gaatcagacg
      481 gtatcaaaat tcactacact actcatgaac ccgactttga ggcacttgca aaattagcca
      541 aagaaattag aaaagagatt caattatgaa aataggtcgt ttaattgcac taggtttagt
      601 tagtttagga gcgcttgaac tttacaaaaa tagaaaaact atcaaggatt cctatcaaaa
      661 tacaaaaaat gagacagatt cagctaaatt aaaattagaa cgtatcaaaa atgatcttgc
      721 tattattagt caggaaaaag aaaaaatcag attaatttct caagaactca atcataaatt
      781 ccaagtgttt aataaagata ttcaacctcg tttagaagaa attaatcaac gcatggctaa
      841 atatcaagag aaagacgaat aagccgacgg tataaaaata tctactctaa atataaaaag
      901 agcaaataat gctctttttt atattttatg gattagtttg acttggtact ggttgcgttc
      961 ctgtgtttgg attaggcgtt gcagccccgt tataattatt aacacttcca cttgaatcag
     1021 cctgacctgt gttagtgtta ctgctatagt tactagcagg agtgctacta ttatagtaac
     1081 tttgatgagt tgtttgatta gtctcagaac tataagtagt attaccactg taacttggtg
     1141 gtgcttctga ataaggtgtt gtattataat tattctcttg tgttgatgaa taagtagaag
     1201 aatcattact agcagtagta ccatagtaat cttcatatag aatcgcgctt gtcttcagag
     1261 ttttactacg ctttttatcc agctctttct taattctatt ttgaactgca agtagatgtt
     1321 tcttagttaa aatttgataa gagccaccat ctgatagagt agcgtcttca cccttcaact
     1381 gataagattt aatatgttcc aatgaatctt tataagctaa caaattagga atcgtttttg
     1441 atgatatctc aatattagtt tgcatgttat tacttactgc ggaaagaatt tttttgtatg
     1501 aactaatact atttaacgcc aatatttttt taaggacttt ttgaattact tcacgttgtc
     1561 ttttttgacg cccataatct ccctctggat catcatagcg catacgagaa taaacaagtg
     1621 cttgttctcc atttatttta tgtgtccctg gttcaacaac agccttgtac tctggttcat
     1681 tggcagcaat tgatattgga aagtcaaatt tattagttac tgttatacca ccaacagcat
     1741 tgaccaaatc aactaatcct tgcatattaa tttgcataaa gtaatcaaca ttaatatcta
     1801 ataagtcttg aacagtcatc aatgccattt ccgcaccacc agaagcatag gctgcattta
     1861 gctttgcttc tacgccagtc tgtccattat ttttgggacc actcaattta atcaatacgt
     1921 cacgttctaa gcttgtcatc gttgttttat tagttttagg atttatagtg actaagatca
     1981 tagaatcgct atttcctgac cacttagatt ttcgatgctc tgaacctgtg tccacaccca
     2041 ttaatagtat tgaaaacggc tttgtttctt caatagcatg actttttgat ttagctagct
     2101 taaaatcttt aaaagtctta gacaattcat tagtcgaaaa attataagct gaggcaacat
     2161 agactcccaa tgcaattact gttgttaaaa taattgcaga aaacattaag gttatttttt
     2221 tccaaatttt catgtatatt cctaatctat caacttactc attctgtaaa tgtctagaaa
     2281 ttctccattt tctcttttaa tacctcttgt ttgaagccca tctatttcaa aactgaattt
     2341 ttggtagagg tgaatagcac gttcattacg tccttggact gatagttcta agcggcgtgt
     2401 aatatcgtta tctgacgccc attctattgc ttcttccatg agaatatgac caataccata
     2461 cccccaataa tccttctgaa cagcaataaa aagatcccca atgtgttcaa tttcaatatc
     2521 agactgacta atgattgtta tcaagccgat cactttattt tttaatttag caatcaaaca
     2581 aatgcaattt ttcgttatta aggtattttc aataaacgtt tccatttctg gcaaagatag
     2641 ttgattgctc accgtatgca aaataaaatc tgtttcctca gtaacagtat ttaaaaattc
     2701 aagaagcgca gatgcttcct ctctcgaagc ttctataaaa tcaacagcaa attgtttaga
     2761 catcttgaat cgcctcaata atttctcttg cgcgatgccc ataagcttct aactgaactt
     2821 gacgtccttg attgctcctc gtaataacaa tttctaagta gttattgata aggttatcac
     2881 taagcaactc tccccattca ataactgtta ctccttgacc aaacaagaag tcatctaagt
     2941 caatagaatc aggatcatct ccaatacgat aaacatccaa atggtaaagt ggtacacgcc
     3001 cttcatattc tctaacaata gtgtaagttg ggctcttaat catttgtttt atgtctaatc
     3061 cctttgcaat accttttgta agagttgttt tgcctgcacc taaattacca gtcaataata
     3121 cgatatctcc tgactttaaa acagtaccca gtttttgccc tagggcaata agctcttctt
     3181 cattttgagt ataaaacata gagatattat accagaaata tctctgtctt ccaacagttt
     3241 gctgattctg acagccccat agcatatatt tcttcacttt tcttaagaaa aaaggaaacc
     3301 aggctacaaa tctgatttcc ttttatctta tttatcaaac tataatatta caaaatagcc
     3361 aagctgataa agtttagaat aaataatata tctaaaatcc acagtaccac atgaatatct
     3421 tttgcttgtc ctttgatgac tttagctagt gtataggtca agaaaccagc tgcaatacca
     3481 taagtaatgc tataagtaaa ccccataaaa agagaagtaa agaaagctgg gatagcttct
     3541 gacatatcat cccatttgat atcttttaaa tttgaaagca tcataatacc aacaataacc
     3601 aatattggtg cagtagcttg actcggaacg atagatacta gtggactaaa aaaactagat
     3661 atagcaaaca aaccagcaac tactaaggct gtcaatcctg tacgaccacc agctccgatt
     3721 cctgctgctg actcaacata ggttgtaacg ttagatgttc cacaaatagc accaaaagtt
     3781 gtcccaatta agtctgaata gagtgctttg tctaaactct tagattcatg attatcacca
     3841 gtcgttgcta agataccaac tttttctccg gtaccaatga gcgttccaat ggtatcaaaa
     3901 atatcagtca gtgaaaaagc aagaatggcc attaatacac ttggtagacg tgaaggatta
     3961 gaaaataaag aaattaatcc tttctctcct aatgcaactc caaatacttg cttcaaatct
     4021 ctaaaagagg ccgagaggtt agttgcttcc caattaatag catctaactt aaccactccc
     4081 attaaaatac ctaagagggt cgttgttaat atcgatagaa taatccctcc acggataccc
     4141 tttacaataa agaagatggt aatgcttaaa ccaatcaaag ataataaaat tgctggatta
     4201 tcaaacgaca ctagccctgg cgttgctgaa gaatttgctg taatagtagc caatcctttc
     4261 gcagcaccct ttccaacaac atcatacgtc ccaggatcaa ttgaaaattt caaaaaacca
     4321 gcatttttaa tcccaacgta tgctaaaaaa gttccgattc ctgctgtaat ggctgatttt
     4381 aaagttgttg ggatagactc aataatcatc tttctaactt tcgttaaagt aataatcaga
     4441 gaaatcaaac cacaaataaa taccatagct aaggcttctt gccatgtata tcccaatgca
     4501 aagacaactg tatatgtaaa gaaagcattt aatcccatcc caggagcttg tgcataggga
     4561 aggttagcat agaaagccat aacagatgta gctacaacgg caccaataat cgtcgctaag
     4621 aaaactcctt gtgctggcat cccagtctga gataaaatag ctggatttac aaacaaaatg
     4681 tacgacattg caaagaaagt cgttagacca gcagtgattt cagtccgaat tgtcgtcccg
     4741 tgttccttta gcttaaaaaa cttttccatt ttaaaatcgg attctccttt attttttgtt
     4801 attttacact tttaaaaaaa ttaatcgttc atatatttac gaacgattaa tatatattaa
     4861 caaaaaataa ttcacttttc aagctttttc cctgcagaat aatccataaa aataagacac
     4921 aatacctgta aaaatgtaaa tcctaaaata aagtggaatg aatccctact cggaaccgct
     4981 aaaagttcgt taattaccac ttgtggcttc gttaagatat cccatgaatt tagacgggca
     5041 taacgaccaa tatgaatacc aaaacttgaa acaaatgata caattccaat aattccccat
     5101 cttaaaaata tattgctaat tctaaattta cgcataatca cagaaaaact ttcgatacct
     5161 gataaaaaac caaacaagat actagataca tacaagatat aaagtattaa atttgtctta
     5221 ttataaagaa cgtctccgac aaaatgcata tgaatgatat ccgtcaacat ataaaaggta
     5281 ttaggataga aaaaaaacca agcaagtgca aaaagaccga taagaatttt tttcttaaat
     5341 aaactagtaa ggtatgcaaa atctaacgct attagggcta gagtcatatt ccaaatcaaa
     5401 tttggtccct gcaaatgata atatttaatc ccaaaagaaa taacaataaa aaataaatgt
     5461 atcgcaaata gttttacttt cattctaacc tccaattaat atctcattat acaacatatt
     5521 attaaattaa ggttaaataa acttcaaacc taaaagacta accctgttgt ggttagtctt
     5581 ccattaatat tccccaaagt aataattgta ctaaaaaaag gcaagacaag gctttaaaat
     5641 caaggttagc catattagtc tgtattccat taaaaaatgc tgacggattt aaaatgattc
     5701 cttctaaacc taatgatcct gatttttcta agcaaaaggc aaaactacta aaaagcatta
     5761 caggtataac agttgctaat tgataccttt tactaatatc atatgcattt accaatagct
     5821 tcaaacttag taaagctgaa gagataataa aaagaattgc aagaaggtat gccatatatt
     5881 gcataaagtg attagactgc caaatgtctt ttgttgacca ctctaagctc actaaactaa
     5941 gaaaaatccc taaactctgt gggtaaaaaa ataaaaggcc taaacttgct aaaagacgaa
     6001 taaaaatatt acgactataa taagcaatga atcccatctc taacgacaag actgatgcca
     6061 atagaattac catcaaatga tcaattttta catgataata ccatatccct aaaatactga
     6121 ctatggcaaa aagatgactc caaattaact ttttcataat ccaccaactt ccatatttcc
     6181 acagttatta tatcatattt ctactgtctc tttttattga aatctatttt tgatataatg
     6241 tcagcatgac aaagaaaatt atcgccgtag atttagacgg tactctatta cataataata
     6301 acactatttc tgactacacc gctgatacac ttcgaaaagt tcaagctcaa ggacacaaag
     6361 taattattac aaccggacgc ccataccgta tggcactcgc tcattattta cgcctagatt
     6421 taaaaacacc aatgataaat tttaacggtg ctttaactca tatccccgaa aaaaagtggg
     6481 cgtttgagca tagtgcaacg attgataaaa aattactcct tgaaacctta aatttatcag
     6541 atgctatcca agccgatttt attgctagcg agtaccgtaa aaatttttac attactatgg
     6601 ataatcgtga taaaattaac ccacaattat tcggtgtcaa tgaaattact gataaaatgg
     6661 cattagatgt tactaaaatc acacgcaatc caaatgctct attgatgcaa acacgtcata
     6721 aagataagta tgagttagct aaagagttac gacaacattt taatcacgaa ctagaagtag
     6781 attcttgggg tggtccattg aatatactcg aattttcacc taaaggtgtt aacaaagctt
     6841 atgctctcaa acatttatta aaatcactca atctttcaca agaaaatttg attgcctttg
     6901 gagatgaaca caatgacacc gagatgctgg cctttgcaca tacaggatat gctatgaaaa
     6961 atgctagtcc aactctactt ccatatgccg accaacaaat ccaatggaca aacgaagaag
     7021 atggtgttgc taaaactcta gaaaaattat t
//
LOCUS       DATRTN010000085         6942 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.085, whole genome shotgun sequence.
ACCESSION   DATRTN010000085 DATRTN010000000
VERSION     DATRTN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6942
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.085"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..600)
                     /locus_tag="VBE22_001680"
     CDS             complement(<1..600)
                     /locus_tag="VBE22_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO7203114.1"
                     /translation="MATSTENLQLAKRGPIISIIAYITLAVAKLATGYWFDATSLVAD
                     GFNNLSDILGNVALLIGLHLASQPADSNHRFGHWKIEDLASLITSFIMFVVGIQVFIQ
                     TVTKIINNTDTNIDPLGAIVGAISALVMLGVYFYNKQLSQRVKSSALVAASKDNLSDA
                     VTSIGTSIAIIAASLNFPIIDRLAAIIITYFILKTAYDIF"
     gene            complement(663..2009)
                     /locus_tag="VBE22_001681"
     CDS             complement(663..2009)
                     /locus_tag="VBE22_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEO7203115.1"
                     /translation="MLTLFTHINSFVWGPPLLALLVGTGIYLSFRLGFVQLRQLSRAF
                     KLIFREDNGQGDISSYAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGLLAIKYRTKDTNGEISGGPMYYIVNGMGQKWKPLAVFFSAAGILVALLGIG
                     TFTQVNAIASSLEHTFKISTRFTSLILAVIVLFIIFGGIKSISKVSEKIVPFMAISYI
                     LATLIIIAVNYNKIPHTFQLIFSGAFSGTAAIGGFSGAIVKEAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTKEPVEQGLISMTGTFIDTIVICTLTGIAILVTGKWLEFDLQGAPL
                     TQASFNTVFGSLGSFALTFCLVLFAFTTILGWSYYGERCFEYLFGTKFINAYRIIFVI
                     MVGLGGFLQLDLIWVIADIVNGLMALPNLIALLVLSPIIVKETQKYFSETKNITKP"
     regulatory      complement(2166..2298)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2605..3930
                     /locus_tag="VBE22_001682"
     CDS             2605..3930
                     /locus_tag="VBE22_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEO7203116.1"
                     /translation="MTLNAKGESNQILVDKEVHSTNLLLDIDEKPELFQGLLLSFQHV
                     FAMFGATILVPLILGMPVSVALFASGCGTLIYQVATKFKVPVYLGSSFAYITAMALAM
                     KQMHGDISAAQTGILFVGLIYVVVATVIKFVGNSWVDKILPPIIIGPMIIVIGLGLAN
                     SAVTNAGFVAKGDWRKMLVAVVTFLIAAFINTKGKGFIKIIPFLFAIIGGYILSIILG
                     LVDLSPVEKADWFELPKFYLPFKTGLFHSYKLYFGPEMLAILPISIVTIAENIGDHTV
                     LGQICGRNFLKKPGLNRLLIGDGLATAFSALIGGPAETTYGENTGVIGMTRIASVTVI
                     RNAAFIAIAFSFFGKFTALISTIPSAVLGGMAILLYGVIASNGLKVLIENRVNFAEVR
                     NLIIASSMLVLGLGGAVLDLGALTLSGTALSAIVGIILNLILPKESMKS"
     gene            4063..4449
                     /locus_tag="VBE22_001683"
     CDS             4063..4449
                     /locus_tag="VBE22_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEO7203117.1"
                     /translation="MKFNLEQVKVFENYEIENWEEGQVTLTTKVVDSSLNYYGNAHGG
                     YLFTLCDQVSGLVAISTGYEAVTLQSNINYLRAGRLDDLLTVIGTCVHNGRTTKVVDV
                     HIKNQKDEMVTTAIFTMFVTGERKEK"
     gene            4555..6834
                     /gene="pcrA"
                     /locus_tag="VBE22_001684"
     CDS             4555..6834
                     /gene="pcrA"
                     /locus_tag="VBE22_001684"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEO7203118.1"
                     /translation="MNPLIIGMNDKQAEAVQTTDGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKYVNPWNILAITFTNKAAREMRERAIALNPATQDTLIATFHSMCVRILRREADYI
                     GYNRNFTIVDPGEQRTLMKRIIKQLNLDTKKWNERSILGTISNAKNDLLDEIAYEKQA
                     GDMYTQVIAKCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNKDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPQAKVVL
                     LEENYRSTKKILQAANNVINHNKNRRPKKLWTQNDEGEQIVYHRANNEQEEAVFVAST
                     IDNIVREQGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIA
                     YLNILANTSDNISFERIVNEPKRGVGPGTLEKIRSFAYEQNMSLLDSSSNVMISPLKG
                     KAAQAVWDLANLILTLRSKLDSLTVTEITENLLDKTGYLEALQVQNTLESQARIENIE
                     EFLSVTKNFDDNPEITVEGETGLDRLSRFLNDLALIADTDDSATETAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRAIEDADELEEERRLAYVGITRAEQILFLTNANTRTLF
                     GKTSYNRPTRFIREIDDELIQYQGLARPVNSSFGVKYSKEQPTQFGQGMSLQQALQAR
                     KSNSQPQVTAQLQALNTNNSHETSWEIGDVATHKKWGDGTVLEVSGSGKTQELKINFP
                     GIGLKKLLASVAPISKKEN"
BASE COUNT         2298 a         1139 c         1323 g         2182 t
ORIGIN      
        1 aaaaatatca taagctgttt taagaataaa ataagtaata atgatagcag ctaaacgatc
       61 aataatagga aaatttagag aagccgcaat gatagcaata gatgtgccga tagatgtcac
      121 agcatcagaa aggttatctt tagaagctgc aaccagagcg ctagacttta ctctttgaga
      181 taattgttta ttatagaaat aaacgccaag cataacaaga gcagatatag cacctactat
      241 agcacctaga ggatcaatgt tagtatcagt attattaata atcttagtga cagtttgaat
      301 aaaaacttga atacctacaa caaacatgat aaaagaggtg atcaggcttg ctaaatcttc
      361 aattttccag tgaccaaaac gatggtttga gtcggctggt tgactagcca agtgtaaacc
      421 aattaagagt gctacatttc ctaagatatc agataagtta ttaaatccat ctgctacaag
      481 tgaagtagca tcaaaccaat aacctgtagc taatttagcg actgccagtg ttatataggc
      541 aatgatacta ataataggcc ctctttttgc aagttgtaag ttttcagtag aagttgccat
      601 ttcctaattt cctttcatct acacattata tcacttagga gcagactata aaactagtaa
      661 gttcaaggct tagtgatatt tttagtctct gaaaaatatt tctgtgtttc tttaacaata
      721 attggtgaca aaactaggag agctattaga tttggtaaag ccattaaacc attaacgata
      781 tcagcgataa cccaaataag atcgagctgt aagaaaccac ctaagccaac cataatgaca
      841 aaaataatac gataagcgtt aataaattta gtcccaaata gatattcaaa gcaacgctct
      901 ccgtagtagc tccatcctaa aatagtagta aaagcaaata agacaaggca gaaggttaga
      961 gcaaaactac ctagtgagcc aaatacggta ttaaaagatg cttgtgttaa tggagcaccc
     1021 tgtaaatcaa attctagcca ttttcctgta actaaaattg caatccctgt caaagtacaa
     1081 ataacaatag tgtcaatgaa ggttcctgtc attgaaataa gcccttgttc aacaggttct
     1141 tttgttttag ctgctgctgc agctattgga gcagaaccca atccagattc atttgagaaa
     1201 actcctctag caattccctt ttgaattgct tctttaacga tagctccaga aaaaccacca
     1261 atggctgctg tcccagaaaa agcaccgcta aagatgagtt ggaaagtatg tggaatctta
     1321 ttgtaattaa ctgcaataat aatcagtgtt gctaaaatat agcttatagc cataaaaggc
     1381 actatttttt ctgaaacttt tgaaatagat tttatacctc caaatataat aaataatact
     1441 ataacagcaa gaatgaggct ggtaaatcta gttgatattt tgaaagtatg ctcaagagaa
     1501 gaggcaattg cattgacttg agtgaatgtc cctattccta aaagagctac taagatacct
     1561 gctgcagaaa aaaagactgc taatggtttc catttttgtc ccattccatt gacaatgtag
     1621 tacattggtc ctccagaaat ttctccatta gtatcttttg tgcgatattt gatagctaat
     1681 aatccttctg cgtattttgt tgccattcca aaaaaggcgg ctacccacat ccaaaacaaa
     1741 gctcctggtc ctccagattt aatagccgta gccacaccaa cgatattacc tgtccctacc
     1801 gttgcagcaa gagcagttgc aagagcagca taacttgaaa tatccccttg tccgttatct
     1861 tctcggaaaa tcaatttgaa agctctagaa agttgtctca attgaacaaa acctaagcga
     1921 aatgataggt aaatacctgt tccgactaat aaagcaagta aaggtggacc ccaaacgaag
     1981 ctattgatat gagtaaaaag tgttagcata tgttctcctt tgatacatga catacctatg
     2041 gattctgtaa tgaaacgaaa aaagttatct ctaaaagaga taacttctga aagatgctag
     2101 ctaaaaagcg cacaaataaa ggtagtagta aacttaataa gcttactact cattattaat
     2161 attggctttt agcttctgtc cttttgcctg agagattgga ggagttgttt tactcttctt
     2221 gccccttcgg cgcctagagc acatcttggt gtcaatacaa agatgttgct acttataggt
     2281 ctctccagaa gtccgtctgt caatcagtcc tgtcttaaga cacctgagag tgtaattcct
     2341 tcggcgaaag tctaactttc ttctcctgaa tgacgtcatt cggtatattt tattatgtta
     2401 actatcataa tgaaaaagca tagttttgtc aatatagttt caaaaaagtc ttgttttcta
     2461 aaatgtctta tgctataatt ctttcagcgt aggtttttaa actcatcccg tgaggtgaga
     2521 aaaacatcag gaaaaaataa agcctatgac tatattgcta tagaaagtgg gcttttttgt
     2581 atgcccaaga ggactagttt agatatgact ttaaatgcta aaggtgaaag taatcaaatt
     2641 ctagtagata aggaggtaca ttctactaac ttattactag atatcgatga aaaaccagaa
     2701 ttatttcaag gtttgttgtt aagttttcaa catgtttttg ccatgtttgg agctacgata
     2761 ttagttccgc ttattttagg gatgcctgta tcagtggctt tatttgcttc tggttgcgga
     2821 acgctcattt atcaagttgc gacaaaattt aaagtccctg tttacctagg ctcatcattt
     2881 gcttatatta cagctatggc attagccatg aaacaaatgc atggcgatat ttcggctgct
     2941 caaacaggta ttctttttgt aggtctaatc tatgtcgttg tggcgacggt tattaagttc
     3001 gtaggaaatt catgggttga taagattttg ccaccaataa tcattggacc tatgattatt
     3061 gttattggtt taggtttggc aaactctgct gtaactaatg caggttttgt cgctaaaggt
     3121 gattggcgta aaatgctagt tgctgttgtt acattcttga ttgcagcttt tatcaatact
     3181 aaagggaaag gttttatcaa gattattcca tttttatttg caattattgg aggttatatt
     3241 ttatcaatca ttcttggctt ggtagattta tctccagttg aaaaagctga ctggtttgaa
     3301 ttaccaaaat tctatttacc gtttaagaca ggtttatttc acagttataa actttacttt
     3361 ggaccagaaa tgcttgcaat tcttccaatc tcaattgtaa cgattgctga aaatatcggt
     3421 gatcatacag ttttaggtca gatttgtggg cgtaactttc taaaaaaacc aggtttaaat
     3481 cgtcttttaa ttggagatgg tttagcaacc gcattttctg ctcttatagg agggccagct
     3541 gaaactactt atggtgaaaa tacaggtgtt attggcatga cgcgtatagc ttctgtaacg
     3601 gttattcgta atgcagcttt tattgcaatc gctttttcat tctttggtaa atttacagct
     3661 ttgatctcta caattcctag tgcagtactt ggtggaatgg ctatattact ttatggtgtt
     3721 attgcaagta atggattaaa agtgttgatt gaaaatcgtg tcaattttgc tgaggttcgt
     3781 aatctgatta ttgccagttc catgttagtt ttaggcctcg gtggagcagt ccttgatcta
     3841 ggtgctctaa ctttatcagg aacagcatta agtgctatag tgggtattat attaaattta
     3901 atattgccaa aagaaagcat gaaatcataa caaaagagct cagagtatct gagctctttt
     3961 gttatgatag atcagttaaa aactttaaaa taaaaacgtt tacagttatg gtttccttat
     4021 tttctgctat aatggtcata gaaaaagtga ggttatagta gaatgaaatt caatttagaa
     4081 caagtaaaag tatttgagaa ttacgaaatt gaaaactggg aagaaggtca agttacctta
     4141 acaacgaagg tcgttgactc ttcgctcaac tattatggta atgctcatgg tggttatttg
     4201 tttacactgt gtgatcaagt cagtggttta gttgctattt caacaggata tgaagcggta
     4261 actctacaat caaatatcaa ctatttgagg gcaggccgat tagatgatct gcttactgtc
     4321 atcggtactt gtgttcataa tggtcgtact acaaaagtcg ttgatgttca cattaaaaat
     4381 cagaaagacg aaatggttac aactgctatt tttacaatgt ttgtaactgg agagagaaaa
     4441 gagaagtaaa aaaggtagga gttagacagc gtctgtttaa ctcctttttc tattgggaaa
     4501 aagatgaaaa aatgatatac tagagaggca attttgacaa aattaggagt agaaatgaat
     4561 cctttaataa ttggaatgaa tgataaacaa gcagaagcgg tacaaacgac agacggaccg
     4621 cttttgatta tggcaggagc tggctctgga aaaacacgtg ttctgactca tcgtattgct
     4681 tatttaatag atgaaaaata tgttaatcct tggaatattt tagcgattac ttttactaat
     4741 aaagcagcgc gtgaaatgcg tgaacgtgct atcgccttga atccagctac ccaagatact
     4801 ttgattgcaa cttttcatag tatgtgtgtt cgtattttac gtcgtgaagc tgattatatt
     4861 ggatataatc gtaactttac aatcgttgat ccaggtgagc agcgtacttt aatgaagcga
     4921 atcattaagc aattaaattt ggacacaaaa aagtggaatg aacgttcaat tttaggtaca
     4981 atctctaatg ctaaaaatga ccttcttgat gaaattgcat atgagaagca agccggagat
     5041 atgtatacac aggtaattgc taaatgctat aaagcttatc aagaggaatt acgtagaagt
     5101 gaggccatgg atttcgatga cttgattatg atgacacttc gattatttga tcagaataaa
     5161 gatgttttgg cctattacca acagaggtat caatatatcc atgtagacga gtatcaagat
     5221 actaaccacg ctcaatacca attagttaag ttattagctt cgcgtttcaa aaatatttgt
     5281 gttgttggtg atgccgatca atccatttat ggatggcgtg gagctgatat gcaaaatatt
     5341 cttgattttg aaaaggacta tccgcaagcc aaagttgtat tattagaaga aaattatcga
     5401 tcaactaaga aaatacttca agctgctaat aatgtgatca atcataataa aaatcgccgt
     5461 cctaaaaaat tatggactca aaatgatgaa ggtgagcaaa ttgtatatca tagagctaac
     5521 aacgagcaag aagaagccgt ttttgtagcg tcaactattg ataacatcgt tcgagaacaa
     5581 ggaaaaaatt tcaaagattt tgccgttctt tatcgtacga atgcgcaatc tcgtactatt
     5641 gaggaagcac ttttaaaatc caatattcca tatacaatgg ttggtggaac aaaattctat
     5701 agtcgtaaag aaattcgaga tgttattgct tatctcaata tccttgcaaa tacttctgat
     5761 aatatttctt ttgagcggat tgtaaatgaa cctaaaagag gggtggggcc aggaacttta
     5821 gaaaaaatac ggtcatttgc ctatgaacag aacatgtctc ttcttgattc ctcttcaaat
     5881 gtcatgatat cgcccttaaa agggaaggct gctcaagctg tttgggattt agctaatctg
     5941 attttgactc tacgtagtaa gctagatagt ttaactgtaa cggagattac agaaaaccta
     6001 ctggataaga caggatatct agaagcactt caagttcaaa atacattaga aagtcaagcg
     6061 cgtattgaaa atattgaaga attcttatca gtgactaaaa attttgacga taacccagag
     6121 attacagtag agggtgaaac tggtttagat cgtctatcgc gttttttaaa tgatttagct
     6181 ttaatagcag atactgatga tagcgccact gaaacggctg aagtcacttt aatgacatta
     6241 catgctgcca agggtctaga atttccagtt gttttcttaa tcggtatgga agaaggagta
     6301 tttcctttat cgcgtgcaat agaggatgct gatgaattag aagaagagcg tcgtttagct
     6361 tatgtcggga ttacgagagc agaacaaata cttttcctta ccaatgctaa tacccgtact
     6421 ttatttggta aaactagtta taatagacca acccgtttta tacgtgagat tgatgatgaa
     6481 ctgatacaat accaagggtt agctcgacca gttaattctt catttggtgt gaaatattca
     6541 aaagaacagc ctacacaatt tggtcaagga atgagtcttc aacaagcgct tcaggcacgt
     6601 aaaagtaatt cacaacctca agtaactgcc caactccagg ctcttaacac gaacaatagc
     6661 catgagacat catgggagat tggtgatgtt gcaactcata aaaaatgggg ggacggtacg
     6721 gtgcttgaag tttcaggtag cggaaagact caagagttaa aaataaattt tccaggtatt
     6781 gggttaaaga aattattagc gagtgtcgct cctattagca aaaaggaaaa ctaactttct
     6841 tctattatat acttaaataa tcagagacta agaattgtat tcttagtctt tttgcataat
     6901 ttgaatagaa aaaataagtt atcatctggt ttttacttgc ta
//
LOCUS       DATRTN010000086         6827 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.086, whole genome shotgun sequence.
ACCESSION   DATRTN010000086 DATRTN010000000
VERSION     DATRTN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.086"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            99..731
                     /locus_tag="VBE22_001685"
     CDS             99..731
                     /locus_tag="VBE22_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEO7203119.1"
                     /translation="MYQELNDYLEFQSMKYIMPEKAGPLKDDMQTFKAKGQLARNSFT
                     ELSRALEQRMDGFKMQRVSNWANQAQVGRPHFWVYYRKDTDQLDDVAVALRVYGVKDS
                     FGVSLEVSFVERQKSDKTLEKQARVLSIPIASPLYFMVQRQGETHREEGNEENRQRLM
                     QEIKSGKVRKVLVKYDVLLTENQSLENILQRLLEGFEKVLPYYQVCQTIS"
     gene            complement(777..2054)
                     /gene="serS"
                     /locus_tag="VBE22_001686"
     CDS             complement(777..2054)
                     /gene="serS"
                     /locus_tag="VBE22_001686"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEO7203120.1"
                     /translation="MLDLKRIRTDFDAVAKKLAMRGVDQETLTTLKELDIKRRELLIK
                     AEEAKAQRNVASAAIAQAKRNKENADEQIAAMQTLSADIKAIDAELADVDANLQSMVT
                     VLPNTPADDVPLGADEDENVEVRRWGTPREFDFETKAHWDLGESLGILDWERGAKVTG
                     SRFLFYKGLGARLERAIYSFMLDEHAKEGYTEVIPPYMVNHDSMFGTGQYPKFKEDTF
                     ELADSPFVLIPTAEVPLTNYYRDEIIDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLNLPYRVITLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEVDGKVRLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGNIDIIKPN"
     gene            2353..3414
                     /locus_tag="VBE22_001687"
     CDS             2353..3414
                     /locus_tag="VBE22_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO7203121.1"
                     /translation="MEKYTSIKQSGRHYGLDLLRIISMFMIVITHILGKGGLRSSVEG
                     HADSYFIVTWIIQVLVYGAVNCYALISGYVGINSRYRYSKLLSIWAQVFFYTFTITAL
                     FEITGHEVTLLNWRDAFFPIVSGQYWYITAYFGLLVFMPVINNGLNALTDKQLKQLVL
                     LMFIIFSILPAVLNNRVPEFSLSKGFEMTWLLILYIIGAYLKRIDLNIFKTSYLLIVY
                     LLSLVATYVMKFSVGDIWYWYVSPTLTLGAVSLFILFARASIKPSGFLKKIIVVLAPS
                     TLGVYLCHLHPLIVKYFVRDFAETFVYESIYLYPFLILGAGILIYLLAFIVERIRLRI
                     FKAIHFSNFLRWFDDKFPY"
     gene            complement(3452..3814)
                     /locus_tag="VBE22_001688"
     CDS             complement(3452..3814)
                     /locus_tag="VBE22_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEO7203122.1"
                     /translation="MAQSLNKTVEFQTTGVSYLGMGNKVGKFLVGDQALEFYNDKNVN
                     DYIQIPWTSINQIGANVSRKKISRHFEVFTDQGKFLFASKDSGTILKHARRHIGDDKV
                     VKLPTLIQTILKIFKFKK"
     gene            complement(3933..4844)
                     /locus_tag="VBE22_001689"
     CDS             complement(3933..4844)
                     /locus_tag="VBE22_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO7203123.1"
                     /translation="MTEQIKLSKSDRQKVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTTKEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEEKASGTPVEDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASAGNILGPIIFFVGWNLIRMSFLWYTQE
                     LGYKSGKEITKDMSGGILQDITKGASILGMFILAVLVKRWVAINFTVDLPKKTLSEGA
                     YINFPKDHVSGQQLHDILGQVQSGLSLDKMQPQTLQGQLDSLIPGLAGLLLTFFCMWL
                     LKKKVSPITIIIGLFIVGILARLAGVM"
     gene            complement(4859..5671)
                     /locus_tag="VBE22_001690"
     CDS             complement(4859..5671)
                     /locus_tag="VBE22_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEO7203124.1"
                     /translation="MADISIISMILVVIVAFFAGLEGILDQFQIHQPLVACTLIGLVT
                     GHLEAGIILGGSLQMVALAWANIGAAVAPDAALASVAAAIILVKSGDFTQKGINFAFS
                     TAVPLAIAGLFLTMIVRTISTALVHAGDKAASEGNFAAIERFHFIALLLQGLRIAFPA
                     ALLLAIPSSSVQSILEAMPDWLNGGMQVGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAALNQLTLIAMGTIGVAIALIYISLSKMGGSKGTSNAGSNDPIGDILEDY"
     gene            complement(5704..6714)
                     /gene="manX"
                     /locus_tag="VBE22_001691"
     CDS             complement(5704..6714)
                     /gene="manX"
                     /locus_tag="VBE22_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEO7203125.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIAQFDADDEVLVLADLWSGSPFNQASRVMGENPERKMAIITGLNLPMLIQ
                     AYTERMMDANAGVEQVAANIIKESKEGIKALPEELNPVVEATPVAGVPADVPAEVKQS
                     GSIPEGTVIGDGKLKINLARIDTRLLHGQVATAWTPASKANRIIVASDEVSKDELRKQ
                     LIKQAAPGGVKANVVPISKLIEVAKDPRFGNTRALILFETVQDALRAVEGGVEIPELN
                     VGSMAHSTGKTMVNNVLSMDKDDVAAFEKLRDLGVSFDVRKVPNDAKKNLFDLINKAN
                     VK"
BASE COUNT         2093 a         1265 c         1315 g         2154 t
ORIGIN      
        1 caacgaggct tcgctagaaa tactttaaaa gaacttgaaa gtatgtatag atatatataa
       61 tcgtgaaaac ttaactattg tttacatgga gaaaaagaat gtatcaagaa ttaaatgatt
      121 atttagaatt ccaatcaatg aagtatatta tgcctgagaa ggcaggccct ttaaaggacg
      181 atatgcaaac ttttaaggca aagggccaac ttgctaggaa tagttttaca gagttatcaa
      241 gagccttaga acaacgtatg gatggcttta aaatgcaaag agtgagtaat tgggctaatc
      301 aagctcaagt agggcgtcca catttttggg tttattatcg aaaggataca gaccaattag
      361 atgatgtggc tgttgcgcta agggtttatg gtgtaaaaga tagttttggg gttagtcttg
      421 aggtaagctt tgtagagagg caaaaatctg ataagacact cgagaagcag gctagagtgt
      481 tatcgattcc gattgcctct ccattgtatt ttatggtcca aaggcaaggt gaaactcatc
      541 gagaagaagg caatgaggaa aatcgtcaac gtttaatgca agaaattaag tcagggaagg
      601 taaggaaagt attggtaaaa tatgacgttc ttttgactga aaaccagagc ttagaaaata
      661 ttctgcaacg attgttagaa ggctttgaga aagtgttacc ttactatcaa gtttgtcaaa
      721 ctattagcta agttcataga aaaaatgagt tggtatacca actcatttta gagtgcctaa
      781 tttggtttaa taatatcgat attacccata tatggacgaa gaacttctgg gatagtcaca
      841 gaaccatctt cattttgata attttcgagg attgctgcta cagtacgtcc aactgcaagt
      901 cctgaaccat ttaaagtatg aagcaaccta actttgccat caacttcgtc acggtaacga
      961 atttgagcac gacgtgcttg gaaatcttca gtattagaac atgaagaaat ttctcgataa
     1021 gtattttgag cgggaatcca tacctctaag tcatacgttt tagctgctga gaatcccata
     1081 tctcctgtac acaaggtaat gacacggtat gggagattaa gtttttgaag aatattttca
     1141 gcatttgctg tcattttttc taattcttgg tacgattcct caggtttggc aaatttcacc
     1201 atttctactt tatggaattg atgaaggcga attaatccac gtgtgtcacg tcctgcagag
     1261 cctgcttctg aacggaatga tggactcatg gcagtaaagt atataggtaa ttctttccca
     1321 tcgataattt catcacgata atagtttgtt aaaggaactt cagcagttgg gataagtacg
     1381 aatgggctat ctgctaattc gaaagtatct tccttgaact ttggatattg tcccgtaccg
     1441 aacattgaat cgtggttaac catataagga gggatcactt cagtgtaccc ttccttagcg
     1501 tgttcatcca gcatgaagct gtagatagca cgctctaaac gagcacctag acctttgtag
     1561 aagaggaagc gagaaccagt aacttttgca ccacgttccc aatcgagaat tccaaggctt
     1621 tcacctaaat cccagtgagc tttggtctca aaatcaaatt cacgaggagt tccccagcga
     1681 cgaacttcaa cattttcatc ctcgtctgct ccaagcggca catcatcagc tggtgtatta
     1741 ggaagtacgg tcaccattga ttgtaaatta gcatccacat ctgccaattc cgcatcaatt
     1801 gctttgatat cagcggaaag tgtttgcata gctgcgattt gctcgtctgc attctcttta
     1861 tttcgttttg cttgagcaat ggcagcagaa gcaacattac gttgcgcttt cgcctcttca
     1921 gctttgataa gtagttcacg gcgttttata tcaagttctt taagggttgt taatgtttct
     1981 tggtcaacac cacgcatggc caatttttta gcgacagcat caaaatctgt acgaatacgt
     2041 tttaagtcta acatatagga atctccttta tactaaaaaa aacaccctgt catgctggag
     2101 cgcaaaggcg cggttccatc cagcttcaca gaagtgcact tgattgattg attaaattat
     2161 ttcccataaa tggaacacgg tagaatttca taaaacaatc ttatctgctc gcaacgaccg
     2221 cagactctct gaaaagaact attatactac ttatccgtta aggttattat atccaaaaat
     2281 aagtaaaatg taaaccttaa ctttataagc ctgctctttg ttttagtgaa atagctatgc
     2341 taaaatatga ttatggaaaa gtatacaagt attaaacaat caggtcgtca ttacggctta
     2401 gaccttttaa ggattatttc catgttcatg attgttatta cgcatattct tggaaaaggt
     2461 ggtctgagat cttcggttga gggacacgca gactcctatt ttatagtaac gtggattatc
     2521 caagtcttag tttatggggc cgttaactgt tacgcactaa taagtggtta tgtgggaatt
     2581 aattcgcgtt atcgttactc aaaattactg tctatctggg cacaggtttt tttctacacc
     2641 tttacaataa cggcactttt tgaaatcaca ggtcatgaag tgaccttgct taattggaga
     2701 gatgcttttt tccctattgt cagtgggcag tattggtata ttactgctta ctttggtcta
     2761 ttggttttta tgcctgttat taataatgga ttaaatgcct tgactgataa acaattaaaa
     2821 caattggttt tgttaatgtt tattattttt tcaattttac cagctgtttt aaacaatcgt
     2881 gttccagaat tttcattgag caagggtttt gaaatgactt ggctactgat tttatatatt
     2941 attggtgcct atttgaaacg tattgattta aatatattta aaacgtcata cttacttata
     3001 gtttatctat tatcattggt tgctacttat gtcatgaagt ttagtgttgg tgatatttgg
     3061 tattggtatg tttcgcctac tttaacttta ggggcggtaa gtttgtttat attatttgct
     3121 agagcaagca ttaagccctc gggattctta aagaaaataa ttgttgtatt agccccttca
     3181 accttgggag tttatctctg tcatctccac ccactaatag taaaatattt tgtaagagat
     3241 tttgcagaga cgtttgttta tgagtccatt tatctttacc catttttaat tttaggagca
     3301 gggattttaa tttatttgtt ggcttttatt gtagaacgta ttagattacg aatatttaaa
     3361 gcaatccatt tttctaactt tttgagatgg tttgatgaca agttcccata ttaatattag
     3421 agttaaaaaa taagatagac tatagtctat cttatttttt aaacttgaat atttttaaaa
     3481 tggtctggat gagggtagga agcttaacaa ctttatcgtc tccaatatgt cgtctcgcat
     3541 gctttaatat cgttccagag tctttagaag cgaataaaaa ttttccttgg tctgtaaaga
     3601 cctcaaagtg acgactaatt ttttttcgac taacattagc tccaatttga ttgattgatg
     3661 tccatggaat ttgaatatag tcgttgacat ttttatcgtt atagaattct aatgcctggt
     3721 caccaactaa aaattttcca actttattcc ccatacctaa atacgaaact cctgttgttt
     3781 gaaattcaac agttttgttt aatgattgtg ccatgactaa tcccttttta atttatagta
     3841 gtttgattat atcataatag tgggactcta agtggatttt tggtaataaa atataagaga
     3901 tggaatacta ttgtaaccat ctcttagttt tattacatca cacctgcaag gcgagctaga
     3961 ataccaacga taaacaaacc gatgataatt gtaattggtg atactttctt cttaagtaac
     4021 cacatacaga agaatgtaag aagtaatcca gctaaaccag gaataagtga gtcaagttgt
     4081 ccttgaagtg tttgtggttg cattttatca aggcttaagc cactctgaac ttgtcctagg
     4141 atatcatgca attgttgtcc actaacatgg tctttaggga aattgatgta tgccccttct
     4201 gacaatgtct ttttaggaag gtctacagta aagttaattg ctacccaacg cttaacaagg
     4261 acagcgagga tgaacatacc aaggattgaa gcacctttag taatatcttg gaggataccg
     4321 ccagacatat ctttagtaat ttctttacct gatttgtaac ctaactcttg tgtgtaccat
     4381 aagaatgaca tacgaatgag gttccaacca acaaagaaga tgattggtcc gaggatatta
     4441 ccagcagagg caagtgaagc accaagagca cctaagatag gacgtacagt aaaccagaac
     4501 actggatcac caataccagc aagaggtccc atcataccga ttttaacccc ttgaattgct
     4561 ttgtcttcga caggtgtacc gcttgctttt tcttcctcaa gagcaagggt tacaccaata
     4621 attggagcag caacgtatgg atgtgtgtta aagaattcca tatggcgctc aagcgcagca
     4681 gcacgatctt ctttagtagt gtaaagtttt ttcaatgctg ggataagagc ataggcccac
     4741 cccatatttt gcatacgctc atagttccaa gaaccttgaa ggaattgtga acgccaccaa
     4801 actttttgac gatctgattt tgataattta atttgttcag tcattgttta gctcctttct
     4861 agtagtcctc taagatatcg ccgataggat cgttagaacc tgcgttagaa gtacctttgc
     4921 ttccacccat ttttgataaa ctgatgtaga tgagggcgat agcaacacca atagtaccca
     4981 ttgcaataag agttaactgg ttcaaagctg ctagagcaaa tccaagagca aagaaaggcc
     5041 aaacttcgcg agtagccatc atattgataa ccatagcgta accaacggca acaaccatgg
     5101 caccaccaac ttgcatacca ccatttaacc aatcaggcat tgcttcaaga atactttgta
     5161 ctgaagaaga tggaatagct aaaagaagcg cagctgggaa tgcaatacgt aatccttgga
     5221 gaaggagggc aataaagtga aaacgttcaa ttgctgcgaa gtttccttca gaagcagctt
     5281 tgtcaccagc gtgaacaagt gcagttgaga ttgtacgaac aatcattgta aggaaaagtc
     5341 cagcaatagc tagaggtaca gcagtagaga aggcaaagtt gatacctttt tgagtgaagt
     5401 cgccactttt aaccaaaata atagcagcag caacagaggc aagtgcagcg tctggtgcta
     5461 cggcagctcc gatatttgcc caagcaagag caaccatttg gagactacca ccaagaataa
     5521 tacccgcttc gaggtgacca gttacaagac cgattaaagt acaagcaaca agaggttgat
     5581 ggatttggaa ttggtcgagg ataccttcaa gaccagcaaa gaaggcaacg ataacgacca
     5641 aaatcattga gataattgaa atatccgcca ttttgatatc ctttcgtttt cagagataat
     5701 aagttatttt acgtttgctt tgttgattaa atcaaacaaa ttctttttag cgtcatttgg
     5761 tactttacgt acatcaaatg atacacctaa gtcacgtaat ttttcaaagg cagcaacatc
     5821 gtctttatcc attgaaagaa cgttgtttac cattgtttta ccagttgagt gagccattga
     5881 accaacgttc aattcaggga tttccacacc accttcaact gcacggagtg catcttgtac
     5941 agtttcaaat aagatcaagg cacgtgtgtt tccgaaacgt gggtctttag ctacttcaat
     6001 taatttacta attgggacaa cgtttgcttt gactcctcca ggagcagctt gcttaatcaa
     6061 ttgtttacga agttcatctt tagaaacttc atcagaagca acgataatac ggtttgcttt
     6121 tgatgctgga gtccatgcag ttgcaacttg accatgtaaa agacgtgtgt caatacgagc
     6181 aagattaatt ttaagcttgc catctccaat aacagttcct tcagggatag atccactttg
     6241 tttaacttcc gcaggaacat ctgcaggaac acctgctaca ggagtagctt ctacaacagg
     6301 attaagttct tctggaaggg ctttgatacc ttccttagac tctttaataa tatttgcggc
     6361 aacttgttca acacctgcat tagcgtccat catacgctca gtataggctt ggataagcat
     6421 tggcaaattg agaccagtga tgatagccat tttacgttca ggattttctc ccataacacg
     6481 gcttgcttgg ttaaatggtg acccactcca aaggtcagct aacacaagca cctcatcatc
     6541 agcatcaaat tgtgcgatag cattgttgaa gtgtccatac aaatcatctg gtccttcatt
     6601 tggcatgaaa gttacaactt gaactttttc ttgttcacca aagatcattg aacctgattg
     6661 atgaatacct tcagcaaatt taccatggct ggcaataata ataccgatac ccattcttgt
     6721 tcttcctcct taaaaaattt tttcgttgac ataaagaaga ataatctcct tccaatcgta
     6781 aaattggcca ttctttttca tttcaacgaa attatagaac agatata
//
LOCUS       DATRTN010000087         6798 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.087, whole genome shotgun sequence.
ACCESSION   DATRTN010000087 DATRTN010000000
VERSION     DATRTN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.087"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            26..748
                     /locus_tag="VBE22_001692"
     CDS             26..748
                     /locus_tag="VBE22_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO7203126.1"
                     /translation="MRLDKFLVECGLGSRTQVKLILKKKQISVNGNSETSPKVQVDEY
                     RDEIKYNGTLVSYEKFVYYMLHKPKGVISATDDPSHKTVLDLLDKTARDKAVFPVGRL
                     DIDTTGLLLITNNGELAHKMLSPKKHVDKCYEAKISGIMTEDDILAFDKGIILKDFTC
                     LPALLEIVEVNQVKKQSLVKITIKEGKFHQVKRMVAACGKEVLELKRLRMGNLQLDKQ
                     LESGQWRRLTIKEIEKLEKYMQ"
     gene            833..2875
                     /locus_tag="VBE22_001693"
     CDS             833..2875
                     /locus_tag="VBE22_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7203127.1"
                     /translation="MKRKYFILNTVTVLTLAAAMNTSSIYANSTETSASVVPTTNTIV
                     QTNDSNPTAKFVSESGQSVIGQVKPDNSAALTTVDTPHHISAPDALKTTQSSPVVEST
                     STKLTEETYKQKDGKDLANMVRSGQVTSEELVNMAYDIIAKENPSLNAVITTRRQEAI
                     EEARKLKDTNQPFLGVPLLVKGLGHSIKGGETNNGLIYADGKISTFDSSYVKKYKDLG
                     FIILGQTNFPEYGWRNITDSKLYGLTHNPWDLAHNAGGSSGGSAAAIASGMTPIASGS
                     DAGGSIRIPSSWTGLVGLKPTRGLVSNEKPDSYSTAVHFPLTKSSRDAETLLTYLKKS
                     DQTLVSVNDLKSLPIAYTLKSPMGTEVSQDAKNAIMDNVTFLRKQGFKVTEIDLPIDG
                     RALMRDYSTLAIGMGGAFSTIEKDLKKHGFTKEDVDPITWAVHVIYQNSDKAELKKSI
                     MEAQKHMDDYRKAMEKLHKQFPIFLSPTTASLAPLNTDPYVTEEDKRAIYNMENLSQE
                     ERIALFNRQWEPMLRRTPFTQIANMTGLPAISIPTYLSESGLPIGTMLMAGANYDMVL
                     IKFATFFEKHHGFNVKWQRIIDKEVKPSTGLIQPTNSLFKAHSSLVNLEENSQVTQVS
                     ISKKWMKSSVKNKPSVMAYQKALPKTGDTESSLSPVLVVTLLLACFSFVTKKNQKS"
     gene            2985..3563
                     /locus_tag="VBE22_001694"
     CDS             2985..3563
                     /locus_tag="VBE22_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7203128.1"
                     /translation="MKVKNKILTMVALTVLTCATYSSIGYADTSDKNTDTSVVTTTLS
                     EEKRSDELDQSSTGSSSENESSSSSEPETNPSTNPPTTEPSQPSPSEENKPDGRTKTE
                     IGNNKDISSGTKVLISEDSIKNFSKASSDQEEVDRDESSSSKANDGKKGHSKPKKELP
                     KTGDSHSDTVIASTGGIILLSLSFYNKKMKLY"
     gene            complement(3597..4838)
                     /locus_tag="VBE22_001695"
     CDS             complement(3597..4838)
                     /locus_tag="VBE22_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEO7203129.1"
                     /translation="MVLQDFDNLLKKYAQLIISKGLNVQKGHTLALTIDVEQVHLARL
                     LTEAAYEKGASEVIVDYTDDFITRQRLLHASDEVLTNVPQYTVDKSLALLNKKASRLV
                     VKSSNPNAFATVDPKRLSETTRATAIALEEQSRAIQANKVSWNVAAAAGREWAALVFP
                     ELKTSDQQVDALWDTIFKLNRIYEDDPIAAWDAHEAKLLEKATRLNQEQFDALHYTAP
                     GTDLTLGMPKNHIWEAAGSLNAQGETFIANMPTEEIFSAPDYRRADGYVTSTKPLSYA
                     GVIIENMTFTFKDGKIINVTAEKGQETVQRLIEENDGARSLGEVALVPHKTPISLSGL
                     IFFNTLFDENASNHLAIGSAYAFNVEGGTEMTSQELDEAGLNRSSTHVDFMIGSEQMD
                     IDGIRADGTAVPIFRNGEWAI"
     gene            5016..5144
                     /locus_tag="VBE22_001696"
     CDS             5016..5144
                     /locus_tag="VBE22_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEO7203130.1"
                     /translation="MAFGDNGQRKKTGFEKLTLFVVILMVLVTVGGLVFGAISAIM"
     gene            5205..6518
                     /gene="obgE"
                     /locus_tag="VBE22_001697"
     CDS             5205..6518
                     /gene="obgE"
                     /locus_tag="VBE22_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEO7203131.1"
                     /translation="MSMFLDTAKISVKAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFKVNEGLRTLMDFRYNRNFKAKAGEKGMTKGMHGRGAEDLIVSLPPGTTVRDATT
                     GKVITDLVEHDQEFVVARGGRGGRGNIRFATPRNPAPEIAENGEPGEERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVVSAAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYDDYVSINNELETYNLRLME
                     RPQIIVANKMDMPDSEENLAAFKEKLAANYDEFDDMPMIFPISSLAHQGLENLMDATA
                     ELLANTEEFLLYDETDMQEDEAYYGFNEDERPFEITRDDDATWVLYGDKLEKLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDIVRIGNFEFEFVD"
     gene            6544..6702
                     /locus_tag="VBE22_001698"
     CDS             6544..6702
                     /locus_tag="VBE22_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203132.1"
                     /translation="MGDKPISFRDKDGNFVSAADVWNAEKLEELFNTLNPNRKLRLER
                     EKLAKEKG"
BASE COUNT         2295 a         1089 c         1367 g         2047 t
ORIGIN      
        1 tatcgaaaga ggttataaca tctaaatgag acttgataaa tttttagtag aatgcggact
       61 tggttcacgt actcaagtca aattaatatt gaaaaaaaag cagattagtg ttaatggtaa
      121 tagcgagaca tctccaaaag tacaggtaga tgaatataga gatgagatta aatataatgg
      181 aactctagtg tcatatgaaa agtttgttta ttatatgctt cataaaccca aaggtgttat
      241 atcagcaacg gatgatcctt cgcataaaac cgtgttagat ttgctagaca aaactgctcg
      301 agataaagct gtttttccag tggggcgttt agatattgat acaacaggcc ttttgttaat
      361 aactaataat ggggaattag cgcataaaat gttatcacct aaaaaacacg ttgataaatg
      421 ctatgaggca aaaatctcag ggataatgac agaagacgat attttggcat ttgataaagg
      481 gattattctg aaagatttta cttgtttacc agcattatta gaaattgtgg aagtaaatca
      541 ggtgaagaag cagagtcttg ttaaaatcac tattaaggaa ggaaaattcc atcaagttaa
      601 acgtatggtt gctgcgtgtg gtaaggaagt cttagagcta aagcgtctaa ggatgggaaa
      661 tctacaatta gataaacagc tagaaagtgg acaatggaga cggctaacaa taaaagaaat
      721 agaaaaatta gaaaaatata tgcaataata taaaacaaca gttaatattt tagcaatttg
      781 ataacactta gattataatg taaacgtata cttttttaga ttggaagatc aaatgaaacg
      841 taaatacttt attcttaata cggtgacggt tttaacgtta gctgctgcaa tgaatactag
      901 cagtatctat gctaatagta ctgagacaag tgcttcagta gttcctacta caaatactat
      961 cgttcaaact aatgacagta atcctaccgc aaaatttgta tcagaatcag gacaatctgt
     1021 aataggtcaa gtaaaaccag ataattctgc ggcgcttaca acagttgaca cgcctcatca
     1081 tatttcagct ccagatgctt taaaaacaac tcaatcaagt cctgtcgttg agagtacttc
     1141 tactaagtta actgaagaga cttacaaaca aaaagatggt aaagatttag ccaacatggt
     1201 gagaagtggt caagttacta gtgaggaact cgttaatatg gcatacgata ttattgctaa
     1261 agaaaaccca tctttaaatg cagtcattac tactagacgc caagaagcta ttgaagaggc
     1321 tagaaaactt aaagatacca atcagccgtt tttaggtgtt cccttgttag tcaaggggtt
     1381 agggcacagt attaaaggtg gtgaaaccaa taatggcttg atctatgcag atggaaaaat
     1441 tagcacattt gacagtagct atgtcaaaaa atataaagat ttaggattta ttattttagg
     1501 acaaacgaac tttccagagt atgggtggcg taatataaca gattctaaat tatacggtct
     1561 aacgcataat ccttgggatc ttgctcataa tgctggtggc tcttctggtg gaagtgcagc
     1621 agccattgct agcggaatga cgccaattgc tagcggtagt gatgctggtg gttctatccg
     1681 tattccatct tcttggacgg gcttggtagg tttaaaacca acaagaggat tggtgagtaa
     1741 tgaaaagcca gattcgtata gtacagcagt tcattttcca ttaactaagt catctagaga
     1801 cgcagaaaca ttattaactt atctaaagaa aagcgatcaa acgctagtat cagttaatga
     1861 tttaaaatct ttaccaattg cttatacttt gaaatcacca atgggaacag aagttagtca
     1921 agatgctaaa aacgctatta tggacaacgt cacattctta agaaaacaag gattcaaagt
     1981 aacagagata gacttaccaa ttgatggtag agcattaatg cgtgattatt caaccttggc
     2041 tattggcatg ggaggagctt tttcaacaat tgaaaaagac ttaaaaaaac atggttttac
     2101 taaagaagac gttgatccta ttacttgggc agttcatgtt atttatcaaa attcagataa
     2161 ggctgaactt aagaaatcta ttatggaagc ccaaaaacat atggatgatt atcgtaaggc
     2221 aatggagaag cttcacaagc aatttcctat tttcttatcg ccaacgaccg caagtttagc
     2281 ccctctaaat acagatccat atgtaacaga ggaagataaa agagcgattt ataatatgga
     2341 aaacttgagc caagaagaaa gaattgctct ctttaatcgc cagtgggagc ctatgttgcg
     2401 tagaacacct tttacacaaa ttgctaatat gacaggactc ccagctatca gtatcccgac
     2461 ttacttatct gagtctggtt tacccatagg gacgatgtta atggcaggtg caaactatga
     2521 tatggtatta attaaatttg caactttctt tgaaaaacat catggtttta atgttaaatg
     2581 gcaaagaata atagataaag aagtgaaacc atctactggc ctaatacagc ctactaactc
     2641 cctctttaaa gctcattcat cattagtaaa tttagaagaa aattcacaag ttactcaagt
     2701 atctatctct aaaaaatgga tgaaatcgtc tgttaaaaat aaaccatccg taatggcata
     2761 tcaaaaagca cttcctaaaa caggtgatac agaatcaagc ctatctccag ttttagtagt
     2821 aaccctttta ttagcttgtt ttagctttgt aacaaaaaag aatcagaaaa gttgattgta
     2881 atgtaaaata attcctattt ttcacttttt agtatcatat tttcgatttt tatgttgtaa
     2941 taaattataa tgaagatgta aggaattata aaataggaaa taatatgaaa gtgaaaaata
     3001 agattttaac gatggtagca cttactgtct taacatgtgc tacttattca tcaatcggtt
     3061 atgctgatac aagtgataag aatactgaca cgagtgtcgt gactacgacc ttatctgagg
     3121 agaaaagatc agatgaacta gaccagtcta gtactggttc ttcttctgaa aatgaatcga
     3181 gttcatcaag tgaaccagaa acaaatccgt caactaatcc acctacaaca gaaccatcgc
     3241 aaccctcacc tagtgaagag aacaagcctg atggtagaac gaagacagaa attggcaata
     3301 ataaggatat ttctagtgga acaaaagtat taatttcaga agatagtatt aagaatttta
     3361 gtaaagcaag tagtgatcaa gaagaagtgg atcgcgatga atcatcatct tcaaaagcaa
     3421 atgatgggaa aaaaggccac agtaagccta aaaaggaact tcctaaaaca ggagatagcc
     3481 actcagatac tgtaatagca tctacgggag ggattattct gttatcatta agtttttaca
     3541 ataagaaaat gaaactttat taaatataaa aaggaggact aacctccttt ttatatttaa
     3601 atagcccatt cgccatttct aaagattggg acagcagttc catctgcacg aataccatca
     3661 atatccattt gttctgaacc aatcataaaa tcaacatgtg ttgaagaacg atttaaacca
     3721 gcttcatcca attcttgact tgtcatttct gttcctcctt ctacattgaa ggcatatgca
     3781 gatccaatag cgaggtgatt agaggcattt tcatcgaata aagtattaaa gaaaatcagt
     3841 ccagatagtg aaattggtgt tttatgtggg acaagtgcaa cttccccaag cgatcttgcc
     3901 ccatcatttt cctcgattaa gcgttggact gtttcttgcc ctttttctgc agtgacattg
     3961 ataattttac cgtctttaaa ggtaaatgtc atattttcga taataacgcc agcataactg
     4021 agaggttttg tacttgtcac atacccatct gcacgacggt aatcaggtgc tgaaaagatt
     4081 tcttcagtag gcatattagc gataaaagtc tctccctgag cgttgagact accggctgcc
     4141 tcccaaatat gatttttagg cataccaagc gttaaatctg tacctggtgc ggtataatga
     4201 agagcatcaa attgttcttg atttagtcta gtagcttttt ctaataattt agcttcatgt
     4261 gcgtcccaag cagcaatagg atcatcttca taaatacgat ttaatttgaa aatggtatcc
     4321 caaagagcat caacttgttg gtcgcttgtt tttaattctg ggaagacaag tgcagcccat
     4381 tctctaccag cagctgcagc cacgttccaa gatactttat tagcttgtat tgctctactt
     4441 tgttcctcta aggcaatagc ggttgctcta gttgtttcag ataaacgttt aggatcaaca
     4501 gtagcgaaag cgttagggtt agaagatttc acaactaatc gactagcctt cttatttaat
     4561 agtgctaaag atttatcaac ggtatactgt ggaacattcg tgagaacttc atctgaagca
     4621 tgaagtagtc gctggcgcgt gataaaatca tctgtataat caacaataac ttcacttgct
     4681 cccttttcat aagcggcttc agttaaaagc cttgctaagt ggacttgttc cacatcgatt
     4741 gttaaagcga gagtgtgccc tttttggaca tttaaacctt tagaaataat taattgggca
     4801 tattttttta aaaggttgtc gaaatcttgt aaaaccatac tagctccttt aaatttacta
     4861 ttttttataa tttatcataa actcttataa agcacaagga aatatggaag cgatttaata
     4921 aagatatatt taagctaacg gtttgtagtt tgcgaaataa gacgtttcgt gttaatatag
     4981 acttaactat atattcagta aatgaggtga catgagtggc ttttggagat aacggacaac
     5041 gaaaaaaaac tgggtttgaa aaactaactc tttttgttgt tattttgatg gtactagtaa
     5101 cagttggtgg tttggtattt ggtgctatta gtgcaatcat gtaatgagaa acgctgtaaa
     5161 gcgttttttt atactgaata gaataaagaa taggaatttt agaaatgagt atgtttttag
     5221 atactgccaa aattagtgtt aaggcaggtc gcggtggtga tggcatggtt gcttttcgcc
     5281 gtgagaaata tgtccccaat ggaggcccgt ggggcggtga tggcggtaaa ggcggatcgg
     5341 tcatttttaa agtaaacgaa ggtttacgaa ctttgatgga ttttcgatat aaccgtaatt
     5401 ttaaagcaaa agctggtgaa aaagggatga ctaaaggtat gcacggtcga ggtgcggaag
     5461 accttattgt gtcattacca cctggaacaa ctgttcgtga tgctactaca ggtaaggtta
     5521 ttaccgacct tgtggagcat gatcaggaat tcgttgtagc cagaggtggc cgcggtggtc
     5581 gtggaaatat tcgctttgca accccacgta atccagctcc tgaaattgct gaaaatgggg
     5641 agcctggtga agaacgtgaa ttacaattag aattaaaaat tttggctgat gttggtctcg
     5701 ttggtttccc atcagttggg aagtcgacgc tacttagtgt tgtttcagca gcaaagccta
     5761 aaattggtgc ctatcatttt acgacaattg tccctaattt agggatggtt cgtacaaaat
     5821 caggtgatag ctttgcaatg gctgatcttc ctggtttgat tgaaggggct agtcaaggtg
     5881 tcggattagg aactcagttt cttagacata ttgagcgtac acgtgttatt ctacatgtta
     5941 ttgatatgtc ggcaagtgaa ggacgagacc cttacgatga ctatgtttca attaataatg
     6001 agttggaaac ttacaatctt cgattgatgg aacgtccgca aattattgtt gccaataaaa
     6061 tggacatgcc tgattcagaa gagaatttgg cagcattcaa agaaaaatta gcagcaaact
     6121 atgatgagtt tgatgacatg cctatgattt ttccaatttc aagtttagca catcaaggac
     6181 tagaaaacct tatggatgcg acggctgaat tattagccaa tacagaagag tttcttcttt
     6241 atgacgaaac tgacatgcaa gaagatgaag catactatgg atttaacgaa gatgaacgtc
     6301 catttgagat tacacgtgat gatgatgcaa cgtgggtatt gtatggtgat aagctagaaa
     6361 aactctttgt tatgacaaat atggaacgcg atgaatcaat aatgaaattt gctcgtcagt
     6421 tacgtggtat gggcgttgat gaagcccttc gagagcgcgg agctaaggat ggagatattg
     6481 ttcgtatagg taacttcgaa tttgaatttg tagattaatg cagtacttga ttggaggttt
     6541 tttatgggag ataaaccgat atcatttcgt gataaagatg ggaattttgt ttcagcggcc
     6601 gatgtctgga atgctgaaaa attagaagaa ctctttaaca cattaaatcc taatcgtaaa
     6661 cttcgtttag aacgcgaaaa attagcaaaa gaaaaaggat agttaagtaa ctatcctttt
     6721 tagttatttt ggtatgaaaa aagactctaa aacataattg atagagtgtt aaggtacaga
     6781 ttactttatt attttaaa
//
LOCUS       DATRTN010000088         6734 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.088, whole genome shotgun sequence.
ACCESSION   DATRTN010000088 DATRTN010000000
VERSION     DATRTN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.088"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            176..4108
                     /gene="srr1"
                     /locus_tag="VBE22_001699"
     CDS             176..4108
                     /gene="srr1"
                     /locus_tag="VBE22_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin Srr1"
                     /protein_id="HEO7203133.1"
                     /translation="MSQKTFGKQLTVVDTKSRVKMHKSGKNWVRTVMSHFNLFKAIKG
                     RATVEADVCIQDVEKEDRLSSGNLTYLKGILAAGALVGGASLTSRVYADETPVVQEQS
                     SSVPTLAEQTEVTVKTTTVQNHQDGTVSKNIIDSNSVSMSESASTSTSESVSMSMSGS
                     TLTSVSESVSTSALTSASESISTSASESVSKSTSISEVSNILETQASLTDKGRESFSA
                     NQIVTESSLVTDAGKNASVSSLIEITKPKSELQTSKMSNESLITPEKSQVMIASDKTG
                     NESLTPTIRLKSVIQPRSMNLMTLSSEMDLIPLEEVSDTEMLGKDVSSELQKVNIALK
                     DNTLSEPGTVKLDSSENLVLNFAFSIASVNEGDVFTVKLSDNLDTQGIGTILKVQDIM
                     DETGQLLATGSYSPLTHNITYTWTRYASTLNNIKARVNMPVWPDQRIISKTTSDKQCF
                     TATLNNQVASIEERVQYNSPSVTEHTNVKTNVRSRIMKLDDERQTETYITQINPEGKE
                     MYFASGLGNLYTIIGSDGTSGSPVNLLNAEVKILKTNSKNLTDSMDQNYDSPEFEDVT
                     SQYSYTNDGSKITIDWKTNSISSTTSYVVLVKIPKQSGVLYSTVSDINQTYGSKYSYG
                     HTNISGDSDANAEIKLLSESASTSASTSASTSASMSASTSASTSASMSASTSASTSAS
                     MSASTSASTSASTSTSTSASTSASTSASTSASMSASTSASTSASTSASTSASTSASMS
                     ASTSASTSASTSASMSASTSASTSASTSASTSASTSTSTSASTSASTSASTSASMSAS
                     TSASTSASTSASMSASTSESISASTSASMSASTSASTSASTSASTSASMSASTSASTS
                     ASTSASTSASMSASTSASTSASTSASTSASMSASTSASTSASTSASTSASTSASTSAS
                     TSASTSSSTSASTSASTSASTSASTSASTSASTSASMSASTSASTSASTSASMSASTS
                     ASTSASTSASTSASTSTSTSASTSASTSASTSASMSASTSASMSASTSASISASTSAS
                     MSASTSASTSASTSASTSASMSASTSASTSASTSASTSASMSASTSASTSASTSASTS
                     ASTSASTSASTSASTSSSTSASTSASTSASTSASMSATTSASTSASMSASTSASTSAS
                     TSASMSASTSSSTSASMSASTSASMSASTSASTSASTSASMSASTSSSTSASMSASTS
                     ASMSASTLASTSASTSASMSASTSASMSATTSASTSVSTSASTSASTSASTSSSSSVT
                     SNSSKEKVYSALPSTGDQDYSVTATALGLGLMTGATLLGRKKSKKDKD"
     gene            4439..5446
                     /locus_tag="VBE22_001700"
     CDS             4439..5446
                     /locus_tag="VBE22_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO7203134.1"
                     /translation="MRVHITSIYGQSPRSIALISQKLVKDVGRQLGYDEMGIYFYNDH
                     AETHGERSTRMDGIIAGLGRGDIVVFQVPTWNSTEFDELFLDKLQAYGARIITFVHDI
                     VPLMFESNFYLLDRVIDMYNRSDVVILPTKAMHDYLIEKGMTTSKVLYQEVWDHPVNI
                     DLPRPECQKVLSFAGDIQRFPFVNDWKENIPLIYYGDGSRLNSEANVHAQGWKDDVEL
                     MLSLSKRGGFGLCWSEDREELVERRYSRMNASYKLSTFLAAGLPIIANHDISSRDFIK
                     QHGLGFTVETLEEAVEKINNMEKETYDSYVENVEKIATLLRNGYITKKLLIDAVHMLY
                     R"
     gene            5455..6660
                     /locus_tag="VBE22_001701"
     CDS             5455..6660
                     /locus_tag="VBE22_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO7203135.1"
                     /translation="MKKTIVLGADFQYRDQVMTTIKSIVSHNQHLTIYIINTDFPVEW
                     FNILNHSLEQFDCRVKNIPISSDVFEGIPTLSHISVAGFFRWFIPIHLEEEIVLYLDS
                     DVIVRGSLDPLFDINLEENLLGAVADHFSTLYYGDTAPVSFNSGVMLINNSLWKKEEI
                     YNSLMRIADKGSAVGVGDQEYLNILTQNRWIDIGKQYNVQIGQDVNINAYGRPDLYHF
                     YDDCEPVIVHYNSQDKPWNKYSQSRYRSEWWYYFGLEWSVIYAQQQKNLNRLTGKTLN
                     LFTLTASDTLEHIEVLAKALPDYQFHIAARSDISEKLANLETYSNIKVYKLVINPILE
                     ELIRNCDVYLDINHSYEVEGILDKIKEENKPILAFDSTAHTTKNIEVISETMPELMVK
                     RIREVGPLR"
     gene            6657..>6734
                     /locus_tag="VBE22_001702"
     CDS             6657..>6734
                     /locus_tag="VBE22_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEO7203136.1"
                     /translation="MKNRKAIALAADFGYQEQVKTIIKSI"
BASE COUNT         2215 a         1290 c         1359 g         1870 t
ORIGIN      
        1 aaatcatact aaaaatcaat tgtaaggttt atatctgtat aaaaatgatg tatctacgtg
       61 cttaacggta tcattatttt tgtgtttaac taagatagat ttctaatcac ttaattttac
      121 tgttatgttt ttaaaccaaa acaaaatata aaaaatattt atatatggag gaaacatgtc
      181 ccaaaagact tttggcaagc agttaacagt tgtagatact aagagtagag tcaagatgca
      241 taaatcagga aaaaactggg taagaacagt aatgtcgcat tttaatctat ttaaagcgat
      301 taaagggaga gcaactgttg aagcagatgt gtgtattcaa gatgttgaaa aagaagaccg
      361 actatcttca ggaaatttga cctatctcaa aggaatacta gctgctggag ctctggtagg
      421 tggagcgagt ttaaccagtc gtgtttatgc agatgagact ccagttgttc aagaacaatc
      481 aagttctgta ccaacactgg cagaacaaac ggaagtgact gttaaaacaa ctactgttca
      541 aaatcatcaa gatgggacag tatcgaaaaa cattattgat tctaatagtg tatctatgtc
      601 agagtcagcc tcaacaagta ctagtgaatc tgtaagtatg tctatgtcag ggtcaacttt
      661 aacaagtgta agtgaatctg taagtacatc tgctttaaca agtgcttcag aatcgataag
      721 cacgtcagcc tcagaaagtg tttcaaaatc tacaagtatt agtgaggttt caaatattct
      781 tgaaactcaa gcttctttaa ctgataaagg aagagagtcg ttttcggcaa accagatagt
      841 aacagaaagt agcttagtta ctgatgctgg taaaaatgct tcagtatcta gcctaattga
      901 aattacaaaa ccaaaatcgg agttacagac ttccaaaatg tcaaatgagt cgcttataac
      961 tccagagaaa tcccaagtaa tgattgcaag cgataaaact gggaatgaga gtctaactcc
     1021 gacaattaga ttaaaatcag ttattcagcc aaggagtatg aacttgatga ctttgagttc
     1081 ggagatggac ttgataccac tagaagaagt gtctgatact gaaatgttag gtaaagatgt
     1141 atcaagcgag ttgcagaaag ttaatattgc gttaaaagat aacactctta gtgagcctgg
     1201 aacagttaaa ttagatagtt cagaaaacct tgttttgaac tttgcctttt caatcgcttc
     1261 tgttaacgag ggagatgtct ttactgtaaa gctttctgat aaccttgaca cacaagggat
     1321 tggtactatt ctaaaagttc aagatataat ggatgaaacg gggcagttat tagcgactgg
     1381 gtcatatagt cctttaacac ataatattac atacacctgg acaaggtatg cttctacgtt
     1441 gaataatatt aaagctagag tcaatatgcc agtttggcct gaccagagaa taatttctaa
     1501 aacaacttca gataagcagt gctttactgc aacattgaac aatcaagttg cttcaattga
     1561 ggaacgtgtt cagtataata gtccttcagt gacagaacat actaatgtta agacaaatgt
     1621 aagatctcgg atcatgaagc ttgatgatga aagacagaca gaaacttata ttactcaaat
     1681 taatcctgaa ggtaaggaaa tgtatttcgc atcaggactt gggaatctat atactattat
     1741 cggttcagat ggaacatcag gttcaccagt taatttatta aatgcggaag taaagattct
     1801 aaaaactaat tcaaaaaatc ttacagatag tatggatcaa aattatgatt cgcctgagtt
     1861 tgaagatgtg acttcccagt atagttatac taacgatggt tctaaaatta ccatagattg
     1921 gaaaacaaat tctatttctt ccactacatc ttatgttgtt ttggtcaaaa tacctaaaca
     1981 aagtggtgta ttgtattcaa ctgtttctga tataaatcaa acatatggtt ctaaatattc
     2041 ttatgggcat acgaatataa gtggtgactc agatgcgaat gccgaaatta aacttttatc
     2101 agaaagtgct tctacgagtg cgtcgacgtc agcaagtacc agcgcttcca tgagtgcctc
     2161 gacatcagca agtaccagcg cttccatgag tgcctcgaca tcagcaagta ccagcgcttc
     2221 catgagtgcg tcgacgtcag ccagcaccag cgcttcaacc agcacctcaa cgagtgcctc
     2281 gacatcagcc agcacaagtg cttcaacaag tgcaagtatg agtgcttcaa caagtgcaag
     2341 taccagcgcc tcaaccagtg ccagtaccag tgcttccacc tcagcaagta tgagcgcctc
     2401 aaccagtgcc agtaccagtg cttccacctc agcaagtatg agcgcctcaa ccagtgcaag
     2461 tactagtgct tcaacaagtg ccagtaccag tgcctcgacg tcaacaagta ctagcgcatc
     2521 cacgtcagca agtaccagtg catccacatc agcaagtatg agcgcatcca cgtcagcaag
     2581 taccagtgca tcgacgtcag caagtatgag cgcctccaca agtgaaagta tcagtgcatc
     2641 gacgtcagca agtatgagcg cttccacaag tgccagtacc agtgcatcca cgtcagcaag
     2701 tactagcgcc tcaatgagtg cctcgacgtc agccagcacc agtgcttcca caagtgcaag
     2761 tactagcgcc tcaatgagtg cctcgacgtc agccagcacc agtgcttcca caagtgcaag
     2821 tactagcgcc tcaatgagtg cctcgacatc agcaagtacc agcgcttcaa caagtgcaag
     2881 tacgagtgct tccacatcag caagtactag cgcctcaaca tcggcaagta caagttcttc
     2941 cacaagtgcc agtaccagtg catcgacatc agccagcact agcgcctcaa ccagtgccag
     3001 taccagtgct tccacctcag caagtatgag cgcctcaaca agtgcaagta ctagcgcttc
     3061 aacaagtgca agtatgagcg cctcaaccag tgcaagtact agtgcttcaa caagtgccag
     3121 taccagtgcc tcgacgtcaa caagtactag cgcttccacg tcagcaagta ccagtgcatc
     3181 cacatcagca agtatgagcg catccacgtc agcaagtatg agcgcctcca caagtgcaag
     3241 tatcagtgca tccacgtcag caagtatgag cgcttccaca agtgcaagta ccagtgcatc
     3301 cacgtcagca agtactagcg cctcaatgag tgcctcgacg tcagccagca ccagtgcttc
     3361 cacaagtgca agtactagcg cctcaatgag tgcatcgaca tcagcaagta ccagcgcttc
     3421 aacaagtgca agtacgagtg cttccacaag tgcaagtact agcgcctcaa catcggcaag
     3481 tacaagttct tccacaagtg caagtaccag tgcatcgaca tcagccagca ctagcgcctc
     3541 aatgagtgcc acgacgtcag ccagcacaag tgcttccatg agtgcgtcga cgtcagccag
     3601 cactagcgcc tcaacgagtg cgtcaatgtc agccagcaca agttcttcaa caagtgcctc
     3661 gatgtcagcc agcactagcg cttcaatgag tgcctcgacg tcagctagca ctagcgcctc
     3721 aacgagtgcg tcaatgtcag ccagcacaag ttcttcaaca agtgcctcga tgtcagccag
     3781 cactagcgct tcaatgagtg cgtcgacgtt agccagcacc agcgcttcaa cgagtgcgtc
     3841 aatgtcagcc agcactagcg cttcaatgag tgccacgacg tcagccagca ccagcgtctc
     3901 aacgagtgca tcgacatcag caagtaccag cgcttccaca agttcttcaa gctcagtgac
     3961 ttctaattca tcaaaagaga aggtgtattc tgccttacct tctacgggtg accaagatta
     4021 ttctgtaact gctactgcct taggtttagg tttaatgact ggtgcaaccc ttttgggacg
     4081 aaaaaaatct aaaaaagata aagactaaac ctactttgaa ttcttaaagt actaaatgac
     4141 atggtaaaag gcatatctct taatattttt agagagatat gcctttttgt tgtagaagtt
     4201 tgtaaaaagc cttgtaaatc tatatttatc aagtgacaag caatgttttt ctgctaaaat
     4261 atgataaaat taagaagtgg aatttatttc tgaaaataag tttatcaatt acgataaact
     4321 caaaaataat gtaaaaaagc tttggatcat tttagtacaa tgagtgttaa taaaactatt
     4381 atttaactgg tatgatatgg tctggataag agagagtttt aaagagagat taactaaaat
     4441 gcgagtacat attacaagta tatatgggca gtcaccgcgt agcattgccc ttatatcaca
     4501 aaaactagtt aaggatgtcg gacgtcaact tggttatgat gaaatgggaa tctactttta
     4561 taatgatcat gctgaaacgc atggtgaaag aagtactagg atggatggta tcattgcggg
     4621 attaggcaga ggggatattg ttgtttttca agtacctaca tggaattcaa cagaatttga
     4681 tgaacttttt ctagataaat tacaggcata cggtgctaga attataactt ttgtacatga
     4741 tattgttccc ttaatgtttg agtctaattt ttatttatta gatagagtaa ttgacatgta
     4801 taatcgttcg gatgttgtta ttctacctac aaaagcgatg catgattatc taattgaaaa
     4861 agggatgact acctctaaag ttctttatca agaagtatgg gatcatccag ttaatataga
     4921 cctccctcgt ccagaatgtc aaaaagtcct tagttttgca ggcgatattc aacgctttcc
     4981 ctttgtcaat gactggaagg aaaatatccc gctcatctat tatggagacg gaagcagact
     5041 caattctgag gcaaatgttc atgctcaggg atggaaagat gatgtagaat tgatgctaag
     5101 tctttctaag cggggaggat ttggcctgtg ttggagtgaa gatagagagg agttagtcga
     5161 gcgtcgttat tcgaggatga atgcctcgta taaattatct actttcttgg ctgctggact
     5221 tccaattatt gctaaccatg atatttcaag tagagacttt atcaaacaac acggattagg
     5281 atttacagtt gaaacgctag aagaagctgt tgaaaaaatt aataatatgg aaaaagagac
     5341 ttatgacagc tatgtggaaa atgttgagaa gatagcaact ctcttgcgaa atggttatat
     5401 cactaaaaaa ttgttgatag atgctgtgca tatgctttat agataggggt agcgatgaag
     5461 aaaacaattg ttttaggtgc agatttccaa tatcgtgatc aagttatgac aactataaaa
     5521 tctattgtta gtcataatca acatttaact atttatatca ttaacacaga ttttcccgta
     5581 gaatggttta atatccttaa ccattcttta gaacagtttg actgtagagt aaaaaatatt
     5641 cccataagtt cagatgtctt tgaggggata cctaccctga gccatatcag tgtggcggga
     5701 tttttcagat ggtttattcc aattcacctt gaagaagaga tagttttata tttagatagt
     5761 gatgtaatag ttaggggttc tttggatccc ctatttgaca taaaccttga agagaattta
     5821 ttaggagctg tagcagatca tttttcaacg ctttactatg gagatacagc accagttagt
     5881 tttaattcag gtgttatgct aatcaataat tcattatgga aaaaagaaga aatttataat
     5941 agccttatga ggattgctga taagggttct gctgttgggg taggagatca agaatacctt
     6001 aatattttga ctcagaatcg ttggatagat atcggtaaac aatataatgt tcagattggt
     6061 caagatgtca atattaatgc atatgggcga ccagatttgt atcattttta tgatgattgc
     6121 gaacctgtta ttgttcatta taatagtcaa gataaaccgt ggaataaata tagtcaatcg
     6181 cgataccgtt cagaatggtg gtattatttt ggtttagaat ggtcagttat ttatgctcaa
     6241 caacaaaaaa atttgaacag gttaacaggt aaaacactga atttatttac attaacagcc
     6301 tcagatacat tagaacatat tgaagtactt gcaaaagctc taccagatta tcagtttcat
     6361 attgcagcac gtagtgatat ttcagagaaa ttagctaatc tagaaactta tagcaatatt
     6421 aaagtttata aattagttat caaccctatc ttggaagagc ttatacgtaa ctgtgatgtg
     6481 tacttggata ttaatcattc ttacgaagta gaaggtattc tagataaaat aaaagaagag
     6541 aataaaccga ttttagcctt tgattctaca gcacatacga caaaaaatat agaagttatc
     6601 tcagaaacaa tgcctgaatt gatggttaaa agaataaggg aagtaggtcc tcttagatga
     6661 aaaatagaaa agctatcgcg ttagcggcag attttggcta tcaagagcaa gttaagacta
     6721 taattaaatc aata
//
LOCUS       DATRTN010000089         6536 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.089, whole genome shotgun sequence.
ACCESSION   DATRTN010000089 DATRTN010000000
VERSION     DATRTN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.089"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..1639
                     /locus_tag="VBE22_001703"
     CDS             <1..1639
                     /locus_tag="VBE22_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEO7203137.1"
                     /translation="FLHHLTNELQEQIEKGRIQKVNQPFDHELVLTIRNNRRNYKLLL
                     SAHPVFGRIQTTEANFQNPQNPNTFTMIMRKYLQGAVIETIQQIENDRILEIIVSNKN
                     EIGDHIKATLVVEIMGKHSNIILIDKNEHKIIESIKHVGFSQNSYRTILPGSTYIAPP
                     KTKAINPFDISDQTLFELLQTNDLSPKNLQQLFQGLGRDTALELSHCLKDNKLNDFRQ
                     FFSREYYPSLTEKSFSAVQFSSSHETFQSLGQLLDYYYQEKAEKDRIAQQASDLIHRV
                     QSELEKNIKKLAKQQDELLATENAEEFRQKGELLTTYLSMVPNNQDVVVLDNYYTNQT
                     IEISLDRALTPNQNAQRYFKKYQKLKEAVKHLKGIISDTENTITYLESVETSLNHASM
                     EDINDIREELVETVFIKRRAHDKQHKRKKPEQYLASDGKTIILVGRNNLQNDELTFKI
                     ARKGELWFHAKDIPGSHVLIRDNLNPSDEVKTDAAELAAYYSKARLSNLVQVDMIEAK
                     KLNKPSGTKPGFVTYTGQKTLRVTPTQEKIDSLKLKK"
     gene            complement(1693..2412)
                     /gene="budA"
                     /locus_tag="VBE22_001704"
     CDS             complement(1693..2412)
                     /gene="budA"
                     /locus_tag="VBE22_001704"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEO7203138.1"
                     /translation="MLETVKLFQYSTLSSLMAGLYKGSLTIGELLTHGDLGIGTVHMI
                     DGELIVLDGKAYQAIGTDGKAEIIQLSDDVTVPYAAVLPHYIQKQFDINAETDNKDLE
                     EMILKNFEGQNLFKSLKIKGTFSRMHVRMIPKSPQHKRFADIASNQPEFTRENVSGTL
                     VGIWTPELFHGVGVKGFHVHFISDDLTFGGHVMDYSLTQGKVEIGKVDQLDQCFPTQD
                     QEFLEANFDLQKLREDIDLSE"
     gene            complement(2426..4108)
                     /gene="alsS"
                     /locus_tag="VBE22_001705"
     CDS             complement(2426..4108)
                     /gene="alsS"
                     /locus_tag="VBE22_001705"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEO7203139.1"
                     /translation="MTESHNQYGADLIVDSLINHDVKYVFGIPGAKIDRVFDTLEDKG
                     PELIVARHEQNATFMAQAVGRITGEPGVVIATSGPGISNLATGLVTATDEGDAVLAIG
                     GQVKRGDLLKRAHQSMNNVAMLEPITKYSAEVHDPNTLSETVANAYRLAKSGKPGASF
                     ISIPQDVTDSPVSVKAIKPLSAPKLGSASVLDINYLAQAINNAVLPVLLLGNGASSEG
                     VTAAVRRLLDAVKLPVVETFQGAGIVSRELEDETFFGRVGLFRNQPGDMLLKRADLVI
                     AIGYDPIEYEARNWNAEISARIIVIDVEQAEIDTYFQPERELIGDMAHTLDLLLPAIK
                     GYELPEGSKEYLKGLRNNIENVSDVKFDRDSAHGLVHPLDLIDVLQENTTDDMTVTVD
                     VGSHYIWMARYFKSYEARHLLFSNGMQTLGVALPWAISAALLRPNTKVISVSGDGGFL
                     FSAQELETAVRLHLPIVHIIWNDGKYNMVEFQEEMKYGRSSGVDFGPVDFVKYAESFG
                     AKGYRVDSKDSFEETLKQALIDAENGPVLIDVPIDYKDNVTLGETILPDEFY"
     gene            complement(4218..5444)
                     /locus_tag="VBE22_001706"
     CDS             complement(4218..5444)
                     /locus_tag="VBE22_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203140.1"
                     /translation="MLNSEKMIVSIQNQDLEHANKYFEKALKNDPEEVLLELGAYLES
                     IGFLPQAKRLYDQIRPNYPEVAINLAQIVAEDGDIEQAFLYLDYISEDSQEYVSALLV
                     MADLYDMEGLTDVAREKLLLASKLSDDPLVTFGLAEMNLSLEHYQEAIEGYASLDNRE
                     ILETTGVSTYQRIGKSYAIMGKFDAAIEFLEKAVDIEYDDLTVFELATILYDQEEYQK
                     ANLYFKQLDTINPDFDGYEYIYGLSLREEHKSEEALRLVQQGLRKNSFDGQLLLLASQ
                     LSYELHDVSSSESYLKQAEKVSENQDEIVMRLSNLYLEEERFEEVLELDNDDLENILA
                     KWNIAKAHKALEMDDSVDYYQSLYNDLKDNPEFLQDYAYILREFGYLDKAQEVGKAYL
                     KLVPDDIEMSEWVNNI"
     gene            complement(5434..>6536)
                     /locus_tag="VBE22_001707"
     CDS             complement(5434..>6536)
                     /locus_tag="VBE22_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO7203141.1"
                     /translation="FLVFLTIFIFTKISFMFKPVFDFLAVLILPLVISGLLYYLLKPM
                     VTFLEKRGIKRVTAILSVFTIIILLLIWAMSSFIPMMSNQLRHFMEDLPSYVNKVQME
                     TSSFIDHNPWLKSYKGEISSMLSNISSQAVSYAEKFSKNVLEWAGNLASTVARVTVAT
                     IMAPFILFYLLRDSRNMKNGFLMVLPTKLRQPTDRILREMNSQMSGYVQGQIIVAITV
                     GIIFSIMYSIIGLRYGVTLGIIAGVLNMVPYLGSFVAQIPVFILALVAGPVMVVKVAI
                     VFVIEQTLEGRFVSPLVLGNKLSIHPITIMFILLTSGAMFGVWGVFLSIPIYASIKVV
                     VKELFDWYKAVSGLYTVDVVTEERSEEVKNVE"
BASE COUNT         2180 a         1354 c         1034 g         1968 t
ORIGIN      
        1 ttttttacat cacctcacaa atgaattaca agaacagatt gaaaaaggac gtatccaaaa
       61 agttaaccaa ccttttgacc atgaattggt attaacaata cgcaataatc gtcgcaacta
      121 taaactactc ctatctgctc accctgtatt tggacgtatc caaactaccg aagcaaattt
      181 ccaaaatcct caaaatccaa atacttttac aatgatcatg agaaagtatt tgcaaggtgc
      241 agtgattgaa accattcaac aaattgaaaa tgaccgtata ttagaaatca tcgtgtctaa
      301 taaaaacgaa atcggtgatc acattaaagc aacacttgtt gttgaaatca tgggaaaaca
      361 cagtaacatt atcttgattg ataaaaatga acataaaatt attgaatcaa ttaagcatgt
      421 tggtttctca caaaactcct atcgtactat ccttcctggt tcgacttaca ttgccccacc
      481 caaaacaaaa gcaatcaatc cttttgatat ttctgatcaa actctatttg agctgcttca
      541 aaccaatgat ctaagcccta aaaacctcca acagctcttc caaggtttag gacgcgatac
      601 tgcgctagaa ttatctcact gtttgaaaga caataaactt aacgattttc gtcaattctt
      661 ttcgagagaa tattatccca gcctaacaga aaaatccttt tccgctgtcc aattctcaag
      721 cagtcacgaa acatttcagt ctcttggaca attgttagat tattactacc aagagaaggc
      781 tgaaaaagat cgcatagcac agcaagctag tgacctcatc caccgtgttc aaagtgagtt
      841 agagaaaaat atcaaaaaac tagctaagca acaagacgaa cttctagcta ctgaaaatgc
      901 agaggagttt cgccaaaaag gagagcttct aactacctat ctctctatgg taccaaataa
      961 tcaagatgtc gttgtgcttg ataattatta caccaaccaa accattgaga tttcacttga
     1021 tcgagcttta acacccaacc aaaatgccca acgctacttt aaaaaatatc aaaaattaaa
     1081 agaagctgta aaacatttaa aaggaattat ttcagatacg gaaaatacaa tcacctacct
     1141 tgaatctgtt gaaacatcgc taaatcatgc ttctatggaa gatatcaatg atattcgtga
     1201 agaacttgtt gaaactgtat ttattaagcg acgcgcacat gataaacaac ataagcgcaa
     1261 aaaacctgaa caatatttag catccgatgg caagactatt attctggtgg gacgtaataa
     1321 tctccaaaat gacgagttaa catttaaaat agctcgtaaa ggggagttgt ggtttcatgc
     1381 caaggatatc cccggaagtc atgtgttaat tcgggataac ctcaatccaa gtgacgaggt
     1441 taagacagat gccgccgagt tagctgctta ttactctaaa gctcgtctct ccaatcttgt
     1501 tcaagtagac atgattgaag caaaaaaact caataaacca agtggtacta agccaggttt
     1561 tgtaacttac actggtcaaa aaaccttacg agtcactccc acacaagaaa aaatagatag
     1621 ccttaaacta aaaaaataga ctgggacata tccctgtcta tttttagtgt tattaattat
     1681 ttataggtat tcttattcac ttaaatcaat atcttctcta agcttttgta ggtcaaagtt
     1741 agcttccaaa aattcttgat cttgagttgg gaaacattgg tcaagttggt caaccttccc
     1801 aatttcaact tttccttgtg tgagcgagta atccataaca tgcccaccga aagttaaatc
     1861 gtctgaaata aagtggacat ggaacccttt tacccccaca ccatgaaata attccggcgt
     1921 ccaaatccca accaacgtac cagatacatt ctctctggta aattctggct gattagatgc
     1981 aatatctgca aaacgtttat gttgtggaga ttttggaatc atacgaacgt gcattcttga
     2041 aaacgttcct ttgattttta aagacttaaa gagattttga ccttcaaagt tttttagaat
     2101 catctcttcc aaatccttat tatctgtctc agcattgata tcaaattgtt tttggatata
     2161 gtgtggtaaa accgctgcat agggtactgt cacatcatct gataattgga taatctctgc
     2221 tttaccatct gtaccaatag cttgataagc tttcccatcc aatacaatca attcaccatc
     2281 aatcatatga acagtaccga ttcctaagtc accgtgagtt aataattctc cgatagttag
     2341 actgcctttg tacaatcctg ccatcagtga actcaaggtg ctgtattgaa ataatttaac
     2401 tgtttctaac atattagacc tcgtcttagt agaactcgtc aggtaagatt gtttcaccta
     2461 gtgtcacgtt atctttatag tcgataggca catcaatcaa cactggtcca ttttcagcat
     2521 cgattagagc ttgtttgagt gtttcttcaa aactgtcttt gctgtctaca cggtaacctt
     2581 tagcaccgaa actttcagcg tattttacaa aatcaactgg tccaaagtca acacctgaag
     2641 aacgcccata cttcatttct tcctggaatt caaccatgtt atattttcca tcgttccaaa
     2701 taatgtgaac aattggtaga tgaagacgaa ctgcagtttc taactcttga gctgagaata
     2761 agaagccacc atcaccagaa actgaaataa cttttgtgtt gggacgcaaa agagccgcag
     2821 aaattgccca aggtagagca actcctaatg tttgcattcc gtttgagaag agcaagtgac
     2881 gcgcttcata tgatttaaag tagcgagcca tccaaatata atgactcccc acatcaactg
     2941 taacagtcat atcatcggtg gtattttctt ggagtacatc aatgagatca agaggatgaa
     3001 caagtccatg agctgagtca cgatcaaatt taacatcgct aacattttca atgttattac
     3061 gaagcccttt caaatactct tttgaacctt caggcaattc atatccttta atagctggta
     3121 aaagtaaatc aagtgtatgt gccatgtctc caatcagttc tcgctcgggt tggaaatacg
     3181 tatcaatttc tgcttgttca acgtcaataa caataatacg agcagaaatt tcagcattcc
     3241 agttacgcgc ttcatattca atagggtcat acccgatagc aataactagg tctgcacgct
     3301 tcaaaagcat atctccaggt tgattacgga agaggcctac acgaccaaag aatgtttcat
     3361 cctcgagctc acgtgacaca atacccgcac cttggaaagt ttctactact ggtagtttaa
     3421 cagcatcaag aagtcgacgt acggcagcag ttactccctc tgaagatgcg ccatttccta
     3481 ataacagtac cggtaaaaca gcattgttaa tagcttgcgc taaataatta atatctaaaa
     3541 cagatgccga acccaattta ggtgcactaa gaggtttgat tgcttttact gagactggac
     3601 tgtctgtaac gtcttgagga atagagataa aacttgctcc tggtttacca gatttagcaa
     3661 gacgataggc gtttgcaaca gtttctgaca aagtattagg gtcatgaacc tcagcagaat
     3721 attttgtaat aggttctagc atagcaacgt tattcattga ttgatgagca cgtttcaata
     3781 aatctcctcg cttaacctgt ccaccaattg caagaacagc atcaccttca tctgtcgcag
     3841 taactaaccc tgttgccaag ttcgagatcc caggtccact agttgcaata acaactcctg
     3901 gttctcccgt aatacgcccg acagcctgag ccataaaagt cgcgttctgc tcatgacgag
     3961 caacaattaa ctctggacct ttatcttcta gagtgtcaaa tacgcggtca atcttagctc
     4021 ctgggatacc aaaaacatat ttgacatcat gattaatgag gctatcgaca attaagtcag
     4081 ccccatattg attatgtgat tctgtcattt tatctccctc ttttcacaag aatggttcaa
     4141 ttatatcaca gtttttacat cagattgtga aaatttactt gtgaattttt tatttttttc
     4201 tgtaagtgtt ttcctattta aatgttattc acccattctg acatctctat atcatctgga
     4261 actaatttta aataagcctt tcctacttct tgtgccttat caaggtaacc aaattctctc
     4321 aaaatgtaag cataatcttg taaaaattct ggattatctt ttaaatcatt atacaaagat
     4381 tgataatagt ctacactatc atccatttct aaagccttgt gtgctttagc gatattccat
     4441 tttgcaagga tattttctaa atcatcattg tctaattcca agacttcctc aaatcgctct
     4501 tcttcaagat acaaatttga caatctcata acaatttcat cttggttttc agaaactttt
     4561 tcagcttgtt tcaaataaga ctccgacgaa gaaacatcat gtaattcata cgataattgt
     4621 gaggctagta agagaagttg accatcaaaa ctatttttac gaagaccttg ctgaactaga
     4681 cgtaaagctt cttcagattt atgctcctca cgaagagata aaccatagat atattcatag
     4741 ccatcaaagt ctggattaat agtatccaac tgtttaaaat aaaggttagc tttttgatat
     4801 tcttcttgat cataaaggat tgttgctaac tcaaaaactg ttaaatcatc atactcaatg
     4861 tcaacggctt tttctaaaaa ttcgatagct gcatcaaatt tccccataat agcgtaggat
     4921 ttaccaatac gctgataagt tgaaacccca gtcgtttcta agatttctcg attgtcaagt
     4981 gaagcatatc cttctatcgc ttcttgataa tgttccaaac taaggttcat ttcagctaaa
     5041 ccaaatgtaa ccaatgggtc atcgctaagt ttgctggcta gcaaaagctt ttcacgcgca
     5101 acatcagtca agccttccat atcatataag tcagccataa caaggagggc tgaaacatac
     5161 tcttgactgt cttctgagat ataatctaag tataagaatg cttgttcaat atctccatct
     5221 tcggcaacaa tttgagctaa attgatagca acttctggat aatttggtcg gatttgatca
     5281 tagagacgtt ttgcttgggg taaaaaacca atactctcca aataagctcc tagttccaat
     5341 aagacttcct caggatcatt tttcaacgct ttttcgaaat acttgttagc atgttctaaa
     5401 tcctgatttt ggatagaaac aatcattttt tcactattca acatttttaa cttcttcact
     5461 tctttcttca gtaacaacat ctactgtata tagcccactg acagctttgt accaatcaaa
     5521 caattcttta acaacaactt tgatagatgc ataaatcgga atactgagga atactcccca
     5581 aacaccaaac atcgctccag aggttaataa aataaacata attgtaattg gatgaatgct
     5641 aagtttatta cctaaaacca agggtgagac aaagcgtccc tctagagttt gctcaataac
     5701 aaaaacaatc gcaactttaa caaccataac aggtcctgcg acaagcgcta agataaacac
     5761 tggaatttgg gcgacaaaac ttcccaaata gggaaccata tttaacacac cggcaataat
     5821 ccctaatgtc acgccatatc taaggcctat aatactatac attattgaaa aaataatacc
     5881 aacagtaata gcaacaatga tttgtccttg cacatatcct gacatttgac tattcatttc
     5941 tcgcaaaata cgatcagttg gttggcgtag tttggttggt aaaaccatta agaaaccatt
     6001 cttcatgttg cgactatctc ttaaaagata aaacaaaata aagggagcca tgattgttgc
     6061 tactgtcaca cgtgcaactg tactagctaa atttcctgcc cactctaaaa cattctttga
     6121 aaatttttca gcataagaga ccgcttggct actgatatta gataacatgc tcgatatttc
     6181 ccctttataa gattttaacc aagggttgtg atctataaac gaacttgttt ccatttgcac
     6241 tttattcaca tatgaaggga gatcttccat aaaatggcgt aattgattac tcatcatggg
     6301 aataaaacta gacattgccc aaattaacag aaggattata atagtaaaaa ctgataatat
     6361 cgctgttaca cgcttaattc cccgcttctc taaaaatgta accataggtt ttaataggta
     6421 ataaagcaat ccagaaatta caagcggcaa tatcagcaca gcaagaaaat caaacacagg
     6481 tttaaacata aaagagattt tggtaaaaat aaaaatcgtt aaaaatacca aaaagg
//
LOCUS       DATRTN010000090         6322 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.090, whole genome shotgun sequence.
ACCESSION   DATRTN010000090 DATRTN010000000
VERSION     DATRTN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.090"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..916
                     /locus_tag="VBE22_001708"
     CDS             <1..916
                     /locus_tag="VBE22_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEO7203142.1"
                     /translation="TIIKLKDKENTRIFICDTITDYETWVKILNDQGIRTESFKEFAY
                     REQLQNKNIEEVMELVDSDLNHYFERVDTQVYLFNDDTLIGRYMVYLGKNYHLIEDGY
                     NCFQAKLFLGGSVVKRVIKTYLFKKYVPYGFSKYCLSIEVNSLVGLPHDIRSKKYKEL
                     PRKKLFDSLNKEQKSLIFKIFKTKPLTITPKSVLLLTQPLAQDKWYKTPTERFQSIQE
                     QYDYFDDIVQEYRTLGYNVYLKVHPRDVVDYSKLPVELLPSNVPMEIIELMSTGRFEC
                     GITHSSTALDSLTCVDKKITLVDLKDIK"
     gene            913..2313
                     /locus_tag="VBE22_001709"
     CDS             913..2313
                     /locus_tag="VBE22_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO7203143.1"
                     /translation="MNTKKLLQSGFIYTLGNLLVQGLAFITLPIYTRVISTEVYGQYS
                     LYVAWMNIIMLFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIILLIAFLFR
                     SFLAPLVGFSESIFLLMVCQSYASYVVTFFGQYFIQQQRSLANLILSLANAVSSVALS
                     LFLIFHWSDDFLSRVFGAFVPTIITGIVAFAYIYYHSKSFYNPKYFRFIVTVSVPLIF
                     HLLGHQLLGQLDRIMLARLYNTKEVAMYSFGYSLGMIIQIVLNSINMAWIPWFFDARK
                     EKLLQLSTYISRYLYLGVFLTLGYLTVFPELAQIMGGDKYSSSVQFISLIIVSYFLVF
                     LYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPHYAAYGAAMATIISYLALLI
                     FHHIVSKVKYHYSDVSVRQYIILSGIVFSYAMLMNMFLDNIVIRWSLGIIILIVYSIV
                     FQKVILDLLSKKRRRR"
     gene            2313..3338
                     /gene="neuB"
                     /locus_tag="VBE22_001710"
     CDS             2313..3338
                     /gene="neuB"
                     /locus_tag="VBE22_001710"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEO7203144.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKETTGTADSQLEMTKRLELSFEEYLEMRDYAISKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAMGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLPEL"
     gene            3415..4569
                     /gene="neuC"
                     /locus_tag="VBE22_001711"
     CDS             3415..4569
                     /gene="neuC"
                     /locus_tag="VBE22_001711"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEO7203145.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEVNLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAVTKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPSLTKEAIEKQLGIVLEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLINHENSYCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETSSVLEG
                     LSKLNEVTNFDNPYYKGNASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     gene            4566..5195
                     /locus_tag="VBE22_001712"
     CDS             4566..5195
                     /locus_tag="VBE22_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO7203146.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDCIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     gene            5206..>6322
                     /gene="neuA"
                     /locus_tag="VBE22_001713"
     CDS             5206..>6322
                     /gene="neuA"
                     /locus_tag="VBE22_001713"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEO7203147.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDAAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPGLFTTLSDKGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLREKSFFTSRTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISISLENYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLITGYSVEEIFSNFQKLYSLLAENKIKMRFTT
                     IAYTLFRETVNNADIEKINQWLTEFCYQNQIPLLDINR"
BASE COUNT         2064 a          933 c         1188 g         2137 t
ORIGIN      
        1 tactataata aagttaaaag ataaagaaaa tactagaata tttatttgtg acactatcac
       61 ggattatgaa acttgggtaa agatattaaa tgatcaggga ataagaactg aatcatttaa
      121 agagtttgct tatcgggagc agttacaaaa taaaaatatt gaagaagtga tggagcttgt
      181 agattcagac ttaaatcact actttgagag agtagataca caagtctatc tatttaatga
      241 tgatacttta ataggtcgct atatggtgta tttaggtaaa aattatcatc taattgaaga
      301 tggttataac tgttttcaag ccaagttatt cttgggtggt tctgtcgtaa aacgagtaat
      361 taaaacttac ttatttaaaa agtatgttcc gtatggtttt tcaaaatatt gtctctcaat
      421 tgaggttaat tctttagtgg ggttacctca tgacataagg agtaagaaat ataaagaact
      481 accgagaaaa aaattatttg atagtcttaa caaagaacaa aaatcactga ttttcaaaat
      541 atttaaaaca aaaccattaa ctataactcc aaagtcagta ttattgttga cacagccact
      601 tgcacaagat aaatggtata aaacacctac agagaggttt caaagtattc aagagcaata
      661 cgattatttt gacgatattg tccaggaata tagaacgtta gggtacaatg tttatttaaa
      721 agttcatcct agagatgtag tagattattc caaattgccg gtagagctat taccatcaaa
      781 tgttcctatg gaaattatag agttgatgtc aacaggtcgg ttcgaatgtg ggataacaca
      841 ttcgtccact gcgctggatt ctttaacttg tgttgataaa aaaataactt tagtagatct
      901 gaaggatatt aaatgaatac taagaaatta cttcaatctg gttttattta tacactcggg
      961 aatttattgg tgcaaggatt agcctttatc acacttccca tctatactcg ggttatctca
     1021 acagaagttt atggccaata tagtttatat gtcgcttgga tgaatattat tatgcttttt
     1081 attggccttc agacgagcgg ttctttaagt tcagcccggg tcaaatacgg agaagaattt
     1141 aaaagctatt cagggagtgc cttttctgta ggtaatatat ggtttcttat tatacttttg
     1201 atagcttttc tatttagaag ttttcttgca ccattagttg gtttttctga atctattttt
     1261 ttattaatgg tgtgtcaaag ttacgctagc tatgtggtga ctttctttgg tcagtatttt
     1321 atacaacaac agaggagttt ggctaattta atattatcct tagccaatgc agtttcatct
     1381 gttgcactat ctctattttt aatttttcat tggtccgatg actttttatc tagggttttt
     1441 ggagcttttg ttcctactat aataactgga atagttgcct ttgcttatat ttattatcat
     1501 agcaaatctt tttacaatcc taagtatttt cggttcattg tcactgtgtc tgttcctttg
     1561 atttttcatt tgttaggaca ccagttgttg gggcaattag atagaattat gctagctcgt
     1621 ttatacaata ctaaagaagt tgcaatgtat agtttcggtt attcgcttgg aatgattatt
     1681 cagattgttt tgaatagtat taatatggct tggattccat ggttttttga tgctagaaaa
     1741 gaaaaattac tgcaactttc aacttatatt agccgttatc tctatttagg agttttctta
     1801 acattaggtt atttgactgt ttttcctgag ttagctcaaa taatgggagg agacaagtat
     1861 agtagtagtg ttcaatttat ttctttaatt attgtcagct actttttagt ttttctctat
     1921 acatttcctg taaatatcca attcttctat gctaatacga catggattcc tattgggaca
     1981 ttattagctg ctggtgtaaa ttggttacta aaccttgtat taattcctca ttatgcagcc
     2041 tatggcgctg ctatggctac aataatatct tatttagcct tgttgatttt tcatcatatt
     2101 gtttcgaaag ttaaatacca ttattcagat gtctcagtta ggcagtacat cattttaagt
     2161 ggtatagtat ttagttatgc tatgttaatg aacatgttcc tagataatat cgttattaga
     2221 tggagtttag gaataattat tcttatagta tatagcattg tttttcaaaa agtcatttta
     2281 gacttattga gtaaaaaaag aaggagaaga taatggttta tattattgca gagattggtt
     2341 gcaatcataa tggagatatt aatcttgcga aaaaaatggt agatgttgcg gtgtcttgtg
     2401 gtgttgatgc tgttaaattt cagactttta aagctgagaa acttatttct aaatttgctc
     2461 ccaaagctga atatcaaaaa gaaactacag gaacagcaga cagtcaactt gagatgacga
     2521 aacgtttaga gttaagcttt gaagaatact tagaaatgcg tgattatgca atttcaaaag
     2581 gtgtggagac cttttcaaca ccttttgatg aagagtcatt agagttctta atttctacag
     2641 atatgccaat ttacaaaatt ccatcaggag aaatcactaa tttaccttac ttagaaaaga
     2701 ttggcaagca acaaaagaaa gttattcttt cgacgggtat ggcggtaatg gaagagatcc
     2761 atcaagcggt gaatatttta cgtcagaatg gtacaactga catttctatt ttacattgta
     2821 caacagagta cccaacacct tacccctctc taaatttaaa cgttattcat actttgaaag
     2881 atgaatttaa agatttaacg ataggttatt cggatcattc aattggatca gaagtaccta
     2941 tcgcagcagc agcaatgggt gcagaagtta ttgaaaaaca ctttacttta gatactaata
     3001 tggaaggtcc ggatcataaa gccagtgcaa cacctgatat tttagctgct ttagttaaag
     3061 gggttcgcat tgttgaacaa gccttaggta gatttgaaaa aatcccagat ccagtagaag
     3121 aaaaaaataa gattgttgct cgtaaatcag tcgttgcttt aaaaccaatt aaaaaaggcg
     3181 atatttattc aatagaaaat attacggtga agcgcccagg taatggtatt tctcctatga
     3241 actggtatga catcttggga caagaagcgc aagatgattt cgaagaggat gaagttattc
     3301 gtgattcacg ctttgaaaat caattgcccg agttataata acacctaatt ataatcggca
     3361 acagatgtat tacacggcta agattatgta attactatat atagaaggga tacgatgaaa
     3421 aaaatttgtt tagtaaccgg atcgcgtgct gaatacggaa taatgaagcc attgattcaa
     3481 agattatcaa aagataaaga agtcaactta caaattattg cgacagcaat gcatctggaa
     3541 gaaaagtacg gctatactta tcgtcaaatt gaagaagacg gttttgatat tgcttataaa
     3601 gttcccttac atctttatga tactgacaga agaactgtat ctactgcaat ggcgcattta
     3661 caactaggat tgaccaaaat ttttgacaag gaagactatg atctagtcat cattttaggg
     3721 gatcgttacg aaatgttacc agttgtgaat gtagcgttga tttataatgt cccagtatgc
     3781 caccttcatg gaggggagac ctcattaggc aattttgatg agtatattcg ccatgcagtt
     3841 actaagatga gtcacctaca cttagtctct acagaggact ttcgtcaacg tgtgattcag
     3901 atgggagaac aacctcaatt tgtaattaac acaggagctc tcggagtgga aaatgctcta
     3961 tcaatccctt ctctaaccaa agaagcaata gaaaagcaat taggtattgt actagaggaa
     4021 tcctattttg ttgttctcta tcatcctgtt acttttgaac aaggaaaaag tgctggagaa
     4081 cagatgaaag cggtactatc agccctttct aaatttggtg tccagtgtct atttatagga
     4141 tccaattcag atactggttc tgatgatatt gcaaaggcta tcaatactta tttgataaat
     4201 catgaaaata gttattgctt tgcttcttta tcaacccaac tttatcattc cttaattcgt
     4261 cattcactag gactgattgg taattcgtct tctggtttaa tagaagttcc ttctttgatg
     4321 aaacctactc ttaatattgg tgatcgccaa aaaggtaggc tacatggaga aagtgtagta
     4381 agtgtaccag tcgaaacctc ctctgtattg gagggattat caaaattgaa tgaagtgacc
     4441 aactttgaca atccctacta caaagggaat gcttcatcta ttgcatatga agcaataaag
     4501 ctttatttga aagatgaacc ttcaatttcc caaccatttt atgatttaaa ggagaataat
     4561 ctaaaatgaa aaaaaagttg gcatttttag gcgcaggaag tcatgctgat gcgattgctc
     4621 cggttattga tcctcttgtg tatgatttcg taggtttttt tgatgataaa gatattacgg
     4681 agcatgatgg ttatcctgtt cttggaaaac tttatgatgt gctaccttac cttgaagatg
     4741 gctcaataga tgcagtattt attacaatag gtgataatgc taaaaggaaa gaactatttg
     4801 aatatgtagc aaaggattat tatgactgta ttattaacat cattagtccc aatgctttag
     4861 tattgacacc agatagtatt tgtgggcgtg gtatctttat tggttttggg gcttttatag
     4921 gttctaaagt gaagctgttt gataacaacg ttgttaatac aggagcgctc attgaacatc
     4981 atactgttgt agaatcacac tgtaatatag cacctaatgc taccataaat ggtctttgtt
     5041 atattagaga agaagtttat gtaggtagtg ccagtgttat tattcaaacc ttggatattt
     5101 catcgtgtac gacaattggc gcaggagctg ttgttgtaaa agatattata gaacctggaa
     5161 cctatgtagg ggttccagcc aaaaaaataa aataaaggat atgttatgaa gccaatttgt
     5221 attattcctg cgcgatcagg gtcaaaagga ttaccagata agaatatgtt attcctagca
     5281 ggtaaaccca tgattttcca tactatagat gctgcaattg aatctggaat gtttgataag
     5341 aaagatattt ttgttagtac agattcagaa ttgtacagag agatttgttt agaacgcggt
     5401 atttcagtgg tgatgagaaa accggaactt tcaactgatc aggcaacttc gtatgatatg
     5461 ttaaaagatt ttttatctga ctatgaagat aatcaggagt ttgtgttact tcaagtaacc
     5521 tctccactaa gaaaatcatg gcatataaag gaagcaatgg agtattattc ttcacatgat
     5581 gttgacaatg ttgtaagttt ttctgaagtt gagaaacacc ctggtctgtt tacgacattg
     5641 tctgataaag gctatgctat agatatggtg ggagcagata aaggttatcg tcgccaagat
     5701 ttacaacctt tatactatcc gaacggcgct atttttattt ctaataaaga aacttactta
     5761 agggaaaaaa gctttttcac ctctaggaca tatgcttatc aaatggcaaa ggaattttca
     5821 ttagatgttg atacgagaga tgattttatc cacgtcatcg gtcacctctt tttcgattat
     5881 gccattaggg aaaaagagaa taaagttttt tataaagaag gctatagtcg tttgttcaat
     5941 agagaagctt caaagataat tttaggtgat tcaaaaacga tatctatctc actagaaaat
     6001 taccataatt attcccaagg tggtgtaaca ttagcaacga tgttggaaaa cttacctaac
     6061 tttttgacag ctaatgtaac agaggctttt gtttctatag gggtaaatga tctcattaca
     6121 ggttatagtg tggaagaaat attcagcaat ttccaaaaac tctactcatt attagctgag
     6181 aataaaataa agatgagatt tacaactatt gcttacaccc tttttagaga aactgtcaat
     6241 aatgcagata ttgagaaaat caatcaatgg ctaacagaat tttgttatca aaatcagatt
     6301 ccactgttag atattaatag at
//
LOCUS       DATRTN010000091         6266 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.091, whole genome shotgun sequence.
ACCESSION   DATRTN010000091 DATRTN010000000
VERSION     DATRTN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6266
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.091"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..1580
                     /locus_tag="VBE22_001714"
     CDS             <1..1580
                     /locus_tag="VBE22_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO7203148.1"
                     /translation="HPLALLLFILYIVILIGFIYTEFAIYTIAILKTEFSLSIKKWLT
                     IFPKKIKSLLGPQLIWVSIYLLLTIPLANLGLRSSILEHLKIPDFISGELTKTVFGKI
                     GYSTLLLLICYLNLRLIYFLPLTILTDYNAKEALLESWKLSRGKHQWRLLSKIILTSL
                     FISVIGTVALAVVAGTSSFIDHSGNNFPLQATFYNLLKSILFAVTLLIKCLIISHLLA
                     DIKQNKSIVQQWRAWKGVQHKQKFKHIACVILALAIGTTALKNAIALALLQDNISIKK
                     ELIAHRGDTSHGVENSIEALKAAHKAKADYSEMDVIMTKDHKLVVIHDDNLKRLSGMN
                     KDVSKLTLDQVTKIPIHQGRFASHIPSFAEFMKTAQSLDQKIMIELKPYNQNLDIYAD
                     EFIKEFKELRLSTKHKVMSLNLTLIEKVEKKLPQLDTGYLIPLHWGTLQNHNVDFYGI
                     EEFSYNDWIAYLAQEYNKQLYVWTINRDNLMIRYLQSPVNGIITDELNLFKVINKDIK
                     NSPNYYQRALQLIDSEG"
     gene            complement(1622..3403)
                     /gene="uvrC"
                     /locus_tag="VBE22_001715"
     CDS             complement(1622..3403)
                     /gene="uvrC"
                     /locus_tag="VBE22_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEO7203149.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKNGTIIYVGKAKNLKNRVKSY
                     FHGSHNTKTELLVSEIEDFEYIVTTSNTEALLLEINLIQENMPKYNIRLKDDKSYPYI
                     KITNERYPRLMITRQVKKSDGTYFGPYPDSGAATEIKRLLDRLFPFKKCTNPANKVCF
                     YYHLGQCNAHTVCQTNKAYWDSLREDVKQFLNGKDNKIVNGLTEKMKSAAMTMEFERA
                     AEYRDLIEAISLLRTKQRVIHQDMKDRDVFGYFVDKGWMCVQVFFVRNGKLIQRDVNM
                     FPYYNEPEEDFLTYIGQFYQDTKHFLPKEVFIPQDIDAKSVETIVGCKIVKPQRGEKK
                     QLVNLAIKNARVSLQQKFDLLEKDIRKTHGAIENLGNLLNIPKPVRIEAFDNSNIQGT
                     SPVAAMVVFVNGKPSKKDYRKFKIKTVIGPDDYASMREVIHRRYSRVLKDGLTPPDLI
                     VIDGGQGQVNIARDVIENQLGLAIPIAGLQKNDKHQTHELLFGDPLEVVELPRNSEEF
                     FLLHRIQDEVHRFAITFHRQLRSKNSFSSKLDGITGLGPKRKQLLMKHFKSLPNIQKA
                     EIEDIIMCGIPRTVAESLRDSLNDPPK"
     gene            3562..4329
                     /locus_tag="VBE22_001716"
     CDS             3562..4329
                     /locus_tag="VBE22_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEO7203150.1"
                     /translation="MIDVENILEKMKPNQKINYDWVMQQMVKQWQASDIRPKILMHVC
                     CAPCSTYTLEYLSQWADVTIYFANSNIHPKDEYYRREYVTQKFVHDFNKNTGYSVQFL
                     SAPYEPNEFFKIVHGLEEEPEGGDRCKVCYDFRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINTIGIDVQKIYDTQYLPSDLKKNKGYQRSVEMCKDYDIYRQCYCGCIFGAKD
                     QGINLLQIKKDAKAFVSDKDGKEEFPNIRFTFNGKSM"
     gene            complement(4388..5728)
                     /locus_tag="VBE22_001717"
     CDS             complement(4388..5728)
                     /locus_tag="VBE22_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO7203151.1"
                     /translation="MYQTQTNKEKFVLFLKLFIPVLIYQFANFSATFIDSVMTGQYSQ
                     LHLAGVSTASNLWTPFFALLVGMISALVPVVGQHLGRGNKEQIRTEFHQFLYLGLILS
                     LILFLIMQFIAQPVLGSLGLEDEVLAVGRGYLNYMLIGIMPLVLFSICRSFFDALGLT
                     RLSMYLMLLILPFNSFFNYMLIYGKFGMPRLGGAGAGLGTSLTYWAIFIVIIIVMSLH
                     PQIKTYHIWTLERIKAPLIIEDIRLGLPIGLQIFAEVAIFAVVGLFMAKFSSIIIAAH
                     QAAMNFSSLMYAFPLSISTALAITISFEVGAERIQDANTYSRIGRLTAVGITSGTLLF
                     LFLFRENVAAMYNSDPHFVAITAQFLTYSLFFQFADAYAAPVQGILRGYKDTTKPFMI
                     GAGSYWLCALPLAVILEKNSQLGPFAYWIGLITGIFVCGLFLNQRLQKIKKLYY"
     gene            complement(5828..6244)
                     /locus_tag="VBE22_001718"
     CDS             complement(5828..6244)
                     /locus_tag="VBE22_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO7203152.1"
                     /translation="MVMKAIEIYMNINGRGAEAITFYEEAFNTKAEHVTTFAQGWPDC
                     SPELEHLIMNAQFNIDGIRMQLSDNNPIFDFQIGQNMTAALIVESVEEAQELFNKLSR
                     DAMNIKMPLQAVPWSPAYGNVTDKYGMTWQINTELS"
BASE COUNT         2134 a         1198 c          939 g         1995 t
ORIGIN      
        1 atcatccctt agctttatta ctctttatac tttatatcgt aatcctaatt gggttcatct
       61 atactgagtt cgctatctat actattgcca tactgaaaac tgaattttct ctcagtatca
      121 aaaaatggct aacaatcttt cctaaaaaaa taaaatcact tttaggacca cagctaattt
      181 gggtatcaat ctacttactt ttgacaattc ctctagctaa ccttggtcta aggtcaagta
      241 tactagagca tcttaaaatt cctgacttta tttctggcga gttaacaaag acagtctttg
      301 gaaagatagg ttactctacc cttttactgc ttatttgtta cttaaacctt cgattaattt
      361 attttctccc gctgaccatt ttaactgact acaatgctaa agaagcactt ttagagagtt
      421 ggaaacttag ccgcggaaag catcagtggc gtcttctaag taaaattatt ttaaccagtt
      481 tatttatatc tgtaattgga acagttgcac tagctgttgt tgctggtaca tcttcattca
      541 ttgatcattc tggaaacaat tttcctcttc aagccacttt ctataacctt ttaaaatcaa
      601 tactttttgc cgtaacactt cttattaaat gtttaatcat aagtcaccta ttagcagata
      661 ttaaacaaaa taaaagtatt gttcaacaat ggagagcttg gaaaggcgtc caacataagc
      721 aaaaatttaa acacattgct tgtgttatcc tagctctagc tattggtacc actgccttaa
      781 aaaatgctat agctctggct ctcctacaag ataacatttc aattaaaaaa gaattaatag
      841 cccatcgtgg tgatacatca catggcgttg aaaattctat tgaagcatta aaagcagccc
      901 ataaagcaaa agcagattat tcagagatgg atgttatcat gacaaaagat cataaattag
      961 tagtcatcca tgatgataat ctcaaacgac tctctggtat gaacaaagat gtttctaaac
     1021 taactttaga tcaagttaca aaaatcccaa ttcaccaagg gcgttttgct agtcacattc
     1081 catcatttgc agaatttatg aagaccgctc agtcactaga tcaaaaaatc atgattgaat
     1141 tgaaaccgta taatcaaaac ctcgatatct atgcggatga atttatcaaa gaatttaaag
     1201 agcttcgtct atcaaccaag cataaagtaa tgtctctcaa cttgacactc attgaaaaag
     1261 ttgagaaaaa attgccacaa cttgatacag gttacctaat cccactacat tggggaacgc
     1321 ttcaaaatca taacgttgac ttttatggga ttgaggaatt ttcatacaat gattggattg
     1381 catatctcgc ccaagaatat aataaacagc tctatgtttg gactattaac cgtgataacc
     1441 ttatgatacg ttatttacaa agcccagtta atggaatcat cacagatgaa ctcaaccttt
     1501 tcaaggtcat taataaagat atcaaaaata gccctaatta ttatcaaaga gctctccaac
     1561 ttattgatag cgagggttaa attaaataaa aagcttagta gaacagtatc tactaagttt
     1621 tttatttagg aggatcatta agagaatctc ggagactttc tgcaacagtc ctaggaatac
     1681 cacacataat aatgtcctca atctcagctt tttggatatt aggtaagctt ttaaaatgtt
     1741 tcattagcaa ttgtttacgt tttggtccca acccagtgat accatctaat tttgaagaaa
     1801 aggaattttt ggagcgtaat tgacgatgaa aagtaatagc aaaacgatgt acctcatctt
     1861 gaatacggtg aagtaagaaa aattcttctg aattacgcgg taattctact acttctaaag
     1921 gatctccaaa aagtaattca tgtgtttggt gtttatcatt cttttgcaaa cctgcaatag
     1981 gtatggctaa acctaattgg ttctcaataa catcacgcgc tatatttact tgcccctgac
     2041 caccatcaat tacaatcaag tccggaggtg tcaagccatc ttttaaaacc ctactataac
     2101 gccgatgaat gacttctctc atactagcat aatcatctgg tccgataaca gtcttaattt
     2161 taaactttcg atagtctttt ttacttggct ttccattcac aaataccacc atagcagcta
     2221 ctggacttgt cccttgaata ttggaattat caaatgcttc tatgcgtact ggtttaggga
     2281 tattaagaag attacctaaa ttttcaattg ccccatgagt tttacgaata tctttttcca
     2341 gtaagtcaaa cttctgttgt aaactgacac gagcattttt tattgccaga tttactaact
     2401 gtttcttttc accacgttgt ggtttaacaa tcttacagcc tacaattgtt tcaaccgact
     2461 tagcatcaat atcctgtgga ataaagactt ctttgggtag aaaatgtttt gtatcttgat
     2521 aaaattgccc tatgtaagtt agaaaatctt cctctggttc attataataa ggaaacatat
     2581 tcacgtcacg ctgaatcaat tttccatttc gaacaaaaaa gacctgtaca cacatccaac
     2641 ccttatcaac gaaataacca aaaacatctc tatccttcat atcttgatga ataacacgct
     2701 gcttagtacg caacaaacta attgcttcga tcaaatctcg atattcagcc gcacgctcaa
     2761 attccatagt catagcagcg ctcttcattt tttcagttaa tccgttaaca attttattat
     2821 ctttaccatt aagaaactgt tttacatctt ctctcagact atcccaataa gctttattag
     2881 tttgacaaac tgtatgtgca ttgcattgcc ctaaatgata ataaaagcat actttatttg
     2941 ctggattagt acatttctta aaaggaaaga gcctatctag taaccgctta atctcagttg
     3001 ctgccccaga atccggataa ggtccaaaat aagtgccatc tgattttttt acttgacgtg
     3061 taatcataag acgaggataa cgttcatttg ttattttgat atatgggtaa gacttatcat
     3121 ctttcaatcg aatattatac ttcggcatat tttcttggat taaattaatt tccaataata
     3181 gagcttcggt attagatgtt gtcacaatgt attcaaagtc ttcaatctca gagaccaaaa
     3241 gttctgtctt agtattatga ctcccatgaa aataactttt cacacgattc ttaagatttt
     3301 tagccttacc tacataaata attgtcccat tcttatcttt atgaaggtaa caaccaggac
     3361 tgtctggtaa caattctagc ttatgtttaa tcagttcatt catagaacta ttataacaaa
     3421 atgtactgta atctttacga aataaatact attccccaac atctattgta tcttccaata
     3481 cttgaataaa accccaatac tcctagtcta agcaattatt ttatgttaca ctttaaacaa
     3541 gtgaattatt gaggagtttt tatgatagac gttgagaata ttcttgaaaa aatgaaacct
     3601 aaccaaaaaa tcaattacga ttgggtcatg caacaaatgg ttaagcagtg gcaagctagt
     3661 gatatccgtc cgaaaattct gatgcatgtt tgctgtgctc cttgtagtac atataccctt
     3721 gaatacttat cacaatgggc agatgttacc atctattttg ctaattcaaa tatccatcct
     3781 aaagatgaat attatcgccg cgaatatgtt actcaaaaat ttgttcatga tttcaacaaa
     3841 aatactggat attctgtaca attcttatca gcaccatatg aaccaaatga atttttcaaa
     3901 attgttcatg ggttagaaga agaacctgaa ggtggggatc gttgtaaagt ttgctatgat
     3961 ttccgtcttg ataagacagc ggaaaaagcc gttgaactag gttttgatta ctttggcagt
     4021 gccctaacta ttagtcctca caaaaactct caaacaatca atactattgg tattgatgtc
     4081 caaaaaattt atgatactca gtatcttcct agcgacctta aaaaaaataa aggttaccaa
     4141 cgttccgttg agatgtgtaa agattatgat atctaccgac aatgttactg tgggtgtatc
     4201 ttcggtgcta aagatcaagg gataaatctt ctccaaatca aaaaagatgc gaaagctttc
     4261 gtgagtgata aagacggcaa agaagaattt cctaatatac gcttcacatt taatggcaag
     4321 tcaatgtaaa tgataaaaaa agacttagta aagaactaag cctttttggt ataaatcttg
     4381 tcaaacttta ataatacaac ttcttaatct tttgcagacg ttggtttaga aatagaccac
     4441 aaacaaaaat acctgtgatt aaaccaatcc aataggcaaa cggacctaac tggctatttt
     4501 tttctaagat aaccgctaat ggcaaagcac ataaccaata agagcccgca ccgatcataa
     4561 atggttttgt tgtatcctta tagcctcgta aaatcccctg tacaggagct gcataagcat
     4621 ctgcaaactg gaaaaagaga ctataagtta ggaattgagc tgtaatagcg acaaagtgag
     4681 ggtcactatt atacattgct gctacattct cacgaaatag aaataaaaat agtaaggttc
     4741 ctgatgtaat ccctaccgct gttaagcgtc ctatcctact ataagtgttt gcgtcctgaa
     4801 tgcgctctgc ccctacttca aacgatattg taatagctag agcagtggaa atacttaaag
     4861 gaaaggcata cattaatgat gaaaaattca tagcagcctg atgtgctgca ataatgattg
     4921 aagaaaattt tgccatgaat aagcctacta ctgcaaaaat tgcaacttct gcaaaaattt
     4981 gtaaaccaat cggtaatccc aatcgaatat cttcaataat caaaggagct tttattcttt
     5041 ccagagtcca tatatgatat gttttaattt gaggatgaag tgacatcaca ataataataa
     5101 caataaaaat agcccaataa gttaaagaag ttccaagacc tgcccccgca cctcctagtc
     5161 taggcatacc aaatttaccg tagataagca tataattaaa aaatgaatta aagggtagaa
     5221 ttaaaagcat cagatacata gataaccttg ttaaccccaa tgcatcaaag aatgaacggc
     5281 aaatgctaaa caacaccagc ggcatgattc caatcaacat ataatttaaa taaccacgac
     5341 caactgctag aacttcatct tctaaaccca aactccccaa gacaggttga gcaataaatt
     5401 gcatgattaa aaataatatt aaggacagta tcaaacctaa atatagaaat tgatgaaatt
     5461 ctgtgcgaat ttgttcttta tttcctctac ccaaatgttg accaactact ggtactaatg
     5521 ctgaaatcat acctactaat aaagcgaaaa acggagtcca taaattacta gcagttgaca
     5581 cacctgccaa atgtagctga ctatactgtc cagtcataac cgaatcaata aaagtagctg
     5641 aaaaattagc aaattgataa atcaatactg ggataaataa tttcaaaaat aaaacaaatt
     5701 tttccttatt tgtctgagtt tgatacatct tttttctcct tttcacctta atcaatttat
     5761 tttagcttag ctaggatact ctgtcaatgc tatttaaaaa ggttgagaaa taatcctcaa
     5821 ccccatttta gctaagctca gtattgattt gccatgtcat accatatttg tcagtcacat
     5881 ttccataagc tggactccac ggaactgctt gtagaggcat tttaatgttc attgcatctc
     5941 gagaaagctt attgaataac tcttgcgctt cttctacaga ttccacaatt aaagctgctg
     6001 tcatattttg tccaatttga aaatcaaaaa taggattatt gtcagaaagt tgcattcgaa
     6061 taccatcaat attaaattga gcgttcataa ttaaatgttc aagttctggc gaacaatctg
     6121 gccaaccctg tgcaaaagtt gtgacgtgtt ctgcttttgt attaaaagcc tcttcataaa
     6181 atgtaatagc ttcggcccca cgaccattaa tattcatata aatttcaatt gctttcatta
     6241 ccatacctca cttcatatta tccaac
//
LOCUS       DATRTN010000092         6216 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.092, whole genome shotgun sequence.
ACCESSION   DATRTN010000092 DATRTN010000000
VERSION     DATRTN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.092"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            23..682
                     /locus_tag="VBE22_001719"
     CDS             23..682
                     /locus_tag="VBE22_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEO7203153.1"
                     /translation="MSLEDYYLLSKKFSQVMTLDSHSSDDGYTIRSLYFDSLEDVDWQ
                     EKEDGVEIRRKIRLRNYGSQSNFAKLEMKQKQGANQKKRSLKLSKTDAQALINGNNSV
                     LLNYPDTFAAECYSRITTQLYQPKTVIEYQRKAFVAKENNIRITFDHHIIGTEASYDI
                     FSEDLNQNYLLDPYLVILEVKYNGFLLSYIKELLDSVDRSELSASKYCLGRAVSKHYR
                     F"
     gene            734..1411
                     /locus_tag="VBE22_001720"
     CDS             734..1411
                     /locus_tag="VBE22_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEO7203154.1"
                     /translation="MKDLLRNSLEQSGNLSFQDMILHILVAALLSVVIYVSYAYTHSG
                     TAYSKKFNVSLMTLTVLTATVMTVIGNNVALSLGMVGALSVVRFRTAIKDSRDTVYIF
                     WTIVVGICCGVGDYVVAALGSSVIFILLWVMGRVKNENRMLLIVKCDRTLEVDLEGIF
                     FQYFDGKAVQRVKNSTTNTIEMIFEISRKDYDKQLHVDNQLTEKVYQLGNIDYFNIVS
                     QSDEING"
     gene            1404..2849
                     /locus_tag="VBE22_001721"
     CDS             1404..2849
                     /locus_tag="VBE22_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEO7203155.1"
                     /translation="MGRLMRGKVIYGTTLIGLFLFLFFYFWIPKHHIERIHHHRIKQV
                     DAKSDLTGFKTHLPIISIDTKQQVIPLVTKEGGKYVKARDNINVDIELRDSPSRSHHL
                     SEKPRIRTKGLISYRGNSSRYFDKKSLKVKFVTNKLKEKKHRLAGMPKESEWVLHGPF
                     LDRTLLRNYLSYNIAGEIMSYAPNVRYCELFVNGEYQGVYLAVENIEQGEQRVPIEKS
                     DKKLHKTPYIVAWDREHKAKQKLDNYVHYTHQSGISALDVKYPGKQRLTSKQLEFINK
                     DINHIEKVLYSYDFSQYPKYIDRESFANYFVINEFFRNVDAGKFSTYLYKDLRDRAKL
                     VVWDFNNAFDNQIEGRVDEADFTLTDAPWFNMLIKDKAFIDLVVHRYKELRKGVLATE
                     YLSNYIDETRHFLGPAIDRNYKKWGYVFDLKNTDPRNYLIPTERNVTSYHKSVEQLKD
                     FIKKRGRWMDRNIETLYQYSAPSKNTNTLLE"
     gene            2856..5015
                     /locus_tag="VBE22_001722"
     CDS             2856..5015
                     /locus_tag="VBE22_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203156.1"
                     /translation="MKKYLSIVALLTILFSSIYYLYYFDGSLYLPKGLLKENTRTNFV
                     VKGDTVLHKPTNKPFVVKGVDVESSLAGYHHNDFPITQKTYREWFHLISNMGANTVRV
                     KVPMNVAFYDALYHHNKASKRPLYLLQGIRIDSYRNNASITAFNDNYRGYLKREAKGV
                     VDILHGRKQVWNTDFGSRHYHYDLSPWVLGYVVGDDWNSGTVAYTNHQEKKTQYKGRY
                     FKTSVAANPFEVMLAQVMDELTHYETAKYGWQHLISFSNSPTTDPFHYRKPFEAQAPK
                     YVQLNVENIQANSNVKAGMFAAYKAIDFHPRYKDYLLFDKENISKEDRQKIKELSLSQ
                     GYVKLLNAYHKIPVLVTGYGYSTARGIAQKEIDKRPLPINEKEQGQRLLEDYESFISS
                     GSFGATINAWQDDWNARAWNTSFATNKHSQFLWGDAQVFNQGYGLLGFKNAKHHYQVD
                     GKRGKGEWKHPLMTSATGDDLYASSDESYLYLAIKTKPEKLKEKRLLPIDITPKSGSR
                     KMNGSKVTFSKSSDFVLSIDPNGKSELFVQERYNALKANYLRQLNGKDFYAFPPKKNS
                     SNFEQINMVLRNTKIVEDMEKVKATERFLPTHPTGLLKTGTTDRHQKTFDSQTDISFG
                     KDFIEVRIPWQLLNFSDPSSQKIHDDYFKHYGVKELEIESIALGLGANSKENTLIKMA
                     DYRLKNWERPDTKTFLKDSYYSIKKEWSKERERTYGT"
     gene            5155..6216
                     /locus_tag="VBE22_001723"
                     /pseudo
     CDS             5155..6216
                     /locus_tag="VBE22_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT         2129 a          903 c         1210 g         1974 t
ORIGIN      
        1 ttagacagga aaaaaatatt taatatcact tgaagattac tatcttttat ctaagaaatt
       61 ttcccaagta atgacgctcg attcgcatag ttcagacgac ggctatacta tccgttcgct
      121 atactttgat tctttagaag atgttgattg gcaagaaaag gaagatggtg ttgagatacg
      181 aagaaagatt cgtttgcgca attatggtag tcaaagcaat tttgcgaaat tagaaatgaa
      241 acaaaaacaa ggtgctaatc aaaaaaagcg ctctctaaaa ttaagcaaga cagatgctca
      301 agcgctgatt aatggtaaca atagtgtttt attaaattat ccggatacat ttgctgcgga
      361 gtgttattca aggataacaa cgcaacttta tcaacctaaa acggttattg agtaccaaag
      421 aaaagctttt gttgctaagg aaaacaatat ccgaatcacc tttgaccacc atataatagg
      481 tacagaggct agttatgata ttttctcaga ggatttaaat caaaattatt tattagatcc
      541 ttatcttgtt attttagaag ttaaatataa tggattttta ttgtcttata ttaaagaact
      601 cttagattcg gtagatagaa gtgaactttc agcgagtaag tattgtttag gaagagctgt
      661 tagtaaacat tatcgttttt agtgaaaaag tatatacgtt aattattatt aaatgtgaga
      721 ggataacaaa aaaatgaaag acctattacg aaatagtcta gagcaaagtg gaaatttaag
      781 ttttcaagat atgattttac atattcttgt agcagcttta ttgagtgtag ttatttatgt
      841 ttcctatgct tatacgcata gtggaactgc ctatagtaaa aagtttaatg tttcattaat
      901 gacattgacg gtcttgactg caacagtaat gaccgttatt ggtaataatg tagccttgtc
      961 attgggtatg gtcggtgcct tgtcagttgt tcgttttagg acagccataa aagattcaag
     1021 agatacagtt tatatttttt ggaccatagt tgttggtatc tgttgtggtg tcggtgacta
     1081 tgtggtagct gcattaggaa gtagcgttat ctttatctta ttatgggtta tgggacgtgt
     1141 taaaaacgag aatcgtatgt tattgattgt gaagtgcgat agaacactag aagttgattt
     1201 agaaggaatt ttcttccaat attttgacgg aaaagctgtt cagcgtgtta aaaattcaac
     1261 aactaatact attgaaatga ttttcgaaat ctctagaaaa gattacgata agcaactcca
     1321 tgtagataat cagttaactg aaaaagtgta ccaattggga aatattgatt atttcaacat
     1381 tgttagccaa agcgacgaaa tcaatgggta gacttatgag agggaaggtt atttacggca
     1441 caacccttat aggtcttttt ctattcttat ttttctattt ttggattcct aagcatcaca
     1501 tcgagagaat acatcatcat cgtataaagc aggtagatgc gaagagtgat ttaacaggat
     1561 ttaaaaccca tttgcccatt atcagcattg atacaaagca acaagttatt cctcttgtta
     1621 caaaagaagg cggaaaatat gtcaaagcta gggataatat taatgttgat atcgaattac
     1681 gggattctcc aagtagatca catcatttat cagaaaagcc gagaattagg acaaaagggt
     1741 taatatcata tagaggaaat tcctctcgtt actttgataa gaagtcattg aaagttaagt
     1801 ttgttactaa taagttaaag gaaaagaagc atcgattagc aggaatgcct aaagaatcgg
     1861 agtgggtatt gcatggtccc tttctagaca gaacattatt aagaaattat ctgagttata
     1921 atattgctgg tgagattatg tcctatgccc caaacgttcg ctactgtgag ttatttgtca
     1981 atggtgagta tcagggagta tatctagcag ttgagaatat cgaacaaggt gagcaacgag
     2041 ttcccattga aaaaagtgat aaaaagttgc ataaaacacc ttatatcgtt gcttgggatc
     2101 gtgaacataa ggcaaagcaa aagttagata actatgtaca ttatacacac cagtctggta
     2161 tctcagctct tgatgtgaaa tatccaggta aacaaagatt gacatcaaaa caattagagt
     2221 ttattaataa ggacatcaat catattgaaa aagtccttta ttcttatgat ttttcacaat
     2281 atcctaaata tattgataga gaatcatttg caaattattt tgtcatcaat gaatttttta
     2341 gaaatgtgga tgcgggtaaa ttttcaacct atctatataa agatcttcgt gatagagcaa
     2401 aactagtagt ttgggatttt aataatgctt ttgataatca aattgaagga cgtgttgatg
     2461 aggcagattt taccttgacg gatgcaccgt ggttcaatat gctcattaag gacaaagcat
     2521 ttatagacct ggttgttcat cgctataaag aattacgaaa gggagttcta gcgacagaat
     2581 atttatctaa ttacatagac gagacaaggc attttttagg tccagctatt gatcgaaatt
     2641 ataaaaaatg gggctatgtc tttgatttaa agaatactga tccaaggaat tatttgattc
     2701 ctactgaacg caatgtgaca agttatcaca aatcagtaga acaattaaaa gattttatca
     2761 aaaagcgtgg cagatggatg gatagaaata ttgagacctt gtatcaatat agtgcaccat
     2821 cgaaaaatac taatacttta ctggagtaga acattatgaa aaaatatcta agtattgtag
     2881 cccttttaac tattttgttt agctctattt attatttgta ttactttgat ggtagtttgt
     2941 atttaccaaa gggcttatta aaagaaaata caagaactaa ctttgttgtt aaaggtgata
     3001 ctgtacttca caagcccacc aataaacctt ttgttgttaa aggagtagac gttgagtctt
     3061 ccttagcggg ttatcatcac aacgattttc ctattactca aaaaacgtat cgtgaatggt
     3121 tccatttaat ttccaacatg ggggcaaata ctgtaagagt caaggtaccg atgaatgttg
     3181 cattttacga tgccttatat caccacaaca aagcatcaaa gaggccactg tatttgttgc
     3241 aaggaatacg tatagattct tatcgcaata atgcttctat aacagctttt aatgataatt
     3301 atagggggta tttaaaacga gaagcaaaag gcgttgtgga tattctccat gggcgtaagc
     3361 aagtatggaa tactgatttt ggtagccgtc attatcatta tgatcttagt ccttgggtac
     3421 ttggttatgt cgtaggggat gattggaata gtggtactgt cgcttatact aatcatcaag
     3481 agaaaaaaac gcaatataaa ggacgttatt ttaaaacttc tgtggcagct aatccatttg
     3541 aggtcatgct agctcaagta atggatgaat tgacacatta tgagacagct aaatatggtt
     3601 ggcaacattt gattagtttt tcaaactcac caacaacaga cccttttcat tatcgaaaac
     3661 catttgaggc acaggctcct aaatacgtac aactaaatgt agaaaatatt caagctaatt
     3721 caaatgttaa agcaggtatg tttgcagcat ataaagctat tgatttccat cctcgataca
     3781 aggattatct attatttgat aaagagaata tcagtaaaga agatagacaa aagattaaag
     3841 aactttcttt gtcacaggga tacgttaaac tgctaaatgc ttatcacaaa atccctgttc
     3901 tagtcacggg ttatggctat tcgacagcga gaggtattgc ccaaaaagaa attgataaac
     3961 gtcctctgcc gattaatgaa aaagaacaag gtcagcgttt actagaagat tatgaatctt
     4021 ttatatcatc cggtagtttt ggagcgacta tcaatgcatg gcaagacgat tggaatgcaa
     4081 gggcgtggaa tacatctttc gccacaaata aacatagtca attcctatgg ggggatgcac
     4141 aagtatttaa tcaaggttat ggtttattag gctttaaaaa cgcaaaacat cattatcaag
     4201 ttgatggtaa aagaggcaaa ggagagtgga aacatcctct gatgactagt gcaacaggag
     4261 atgacttata tgctagcagt gatgaaagct atctctacct tgcgattaaa acaaaacctg
     4321 aaaaactaaa agaaaaacga ttattaccaa tagatattac accaaaatct ggtagtagaa
     4381 aaatgaatgg tagtaaggtc acattttcta aatctagtga ctttgtattg tctattgatc
     4441 caaatggcaa gtctgaatta tttgtccaag agcgctataa tgccttaaaa gcgaactatc
     4501 ttcgacagct taacggtaaa gatttttatg ctttcccacc aaagaagaac agtagtaatt
     4561 ttgagcagat aaatatggta ttgagaaata caaagattgt tgaagacatg gaaaaagtaa
     4621 aagcaacaga gaggttctta ccaactcatc ctactggtct tctcaaaaca ggaacaactg
     4681 ataggcacca aaaaacattt gattcacaaa cagatatttc gtttggaaag gactttatag
     4741 aggtcagaat tccgtggcag ttgttgaatt tttctgatcc atcatctcaa aaaattcacg
     4801 atgattactt taaacattat ggtgtgaagg agttagaaat tgagagcatt gctttaggat
     4861 taggtgctaa tagcaaagaa aacacactga taaagatggc agattatcgt ttgaaaaatt
     4921 gggagagacc cgataccaaa acctttttaa aagactccta ttatagtatt aagaaagaat
     4981 ggtctaaaga aagagagaga acatatggta cataatattt tatattttta cattttgatt
     5041 tgcttagcaa ttattgtttt taacattcac tatatcttaa gacaacgatt agataagctt
     5101 agttggcttc gtattcggaa aaaggatata aatatcaaat aaaggggatt ttctatggca
     5161 ttatttttta ccttcctaaa ggtatctcaa gtcattttct tagtattatt gattgcctat
     5221 gccagctttt tagtgatttc attagtcatt ggaggtgttc atctctatga aaaaaatgag
     5281 cgcaaaaaaa gtttaactag aaataaagaa aaaattccaa tttcaattat tgtaccagcc
     5341 tataatgaat caacaaccat tgtgagttcg attgattcgt tattgcacct tgattatgaa
     5401 gcgtatgaaa ttatcgttgt ggatgacggt tccagtgaca atacttcaga tgttctcaag
     5461 gaagagtttg cattaatgaa aatctcgaat acgattgatt caattattgc tactcaaact
     5521 tgtaaagatg tctttcagag acaggttggt aaggttaaac tgacactcat tgttaaagaa
     5581 aatggtggca aaggggatgc cttaaatatg gggatcaatg ctgctaatta cgattatttt
     5641 ctatgtctag atgcagattc tatgttgcag gtagattctt tgagtcaaat tagcaaatcc
     5701 atacaggtag atccgacggt tatagcagtg ggagggctcg tccaagttgc ccaaggtgtt
     5761 aagattgagc aggggaaagt agcatcttat cgtttgcctt ggagaattat tccttgtgca
     5821 caagcacttg agtatgattc atcattttta ggagctcgaa tctttttaga ctatttaaga
     5881 gctaatttaa ttatttcggg ggctttcggt ctttttaaga aagaccttgt taaagcggtg
     5941 ggaggatatg acacccagac tctaggagaa gatatggagt tggtgatgaa attacatttc
     6001 ttctgtcgta ataataatat tccttatcgt atttgttatg aaacagatgc tgtttgttgg
     6061 agtcaagcac caacgaattt aggggattta cgtaaacaga gacgccggtg atatcttggc
     6121 ttgtaccaat gtttaaaaaa atataaaagt atttttgcca attatcgctt tggagctgta
     6181 ggatcaattt cttatattta ctatatttta tttgaa
//
LOCUS       DATRTN010000093         6159 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.093, whole genome shotgun sequence.
ACCESSION   DATRTN010000093 DATRTN010000000
VERSION     DATRTN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.093"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            17..2089
                     /locus_tag="VBE22_001724"
     CDS             17..2089
                     /locus_tag="VBE22_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO7203157.1"
                     /translation="MKKKIILKSSVLGLVAGTSIMFSSVFADQVGVQVIGVNDFHGAL
                     DNTGTANMPDGKVTNAGTAAQLDAYMDDAQKDFKQTNPNGESIRVQAGDMVGASPANS
                     GLLQDEPTVKTFNAMNVEYGTLGNHEFDEGLAEYNRIVTGKAPAPDSNINNITKSYPH
                     EAAKQEIVVANVIDKVNKQIPYNWKPYTIKNIPVNNKSVNVGFIGIVTKDIPNLVLRK
                     NYEQYEFLDEAETIVKYAKELQAKNVKAIVVLAHVPATSKDDIAEGEAAEMMKKVNQL
                     FPENSVDIVFAGHNHQYTNGLVGKTRIVQALSQGKAYADVRGVLDTDTQDFIETPSAK
                     VIAVAPGKKTGSADIQAIVDQANTIVKQVTEAKIGTAEVSGMITRSVDQDNVSPVGSL
                     ITEAQLAIARKSWPDIDFAMTNNGGIRADLLIKPDGTITWGAAQAVQPFGNILQVVEI
                     TGRDLYKALNEQYDQKQNFFLQIAGLRYTYTDNKEGGEETPFKVVKAYKSNGEEINPD
                     AKYKLVINDFLFGGGDGFASFRNAKLLGAINPDTEVFMAYITDLEKAGKKVSVPNNKP
                     KIYVTMKMVNETITQNDGTYSIIKKLYLDRQGNIVAQEIVSDTLNQTKSKSTKINPVT
                     TIHKKQLHQFTAINPMRNYGKPSNSTTVKSKQLPKTNSEYGQSFLMSVFGVGLIGIAL
                     NTKKKHMK"
     gene            complement(2126..2536)
                     /locus_tag="VBE22_001725"
     CDS             complement(2126..2536)
                     /locus_tag="VBE22_001725"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO7203158.1"
                     /translation="MIKPIVRDTFFLQQKSQMASRADVSLAKDLQDTLHANQNYCVGM
                     AANMIGSLKRVIIINVGITNLVMFNPVLVAKSDPYETEESCLSLVGCRSTQRYHHITI
                     SYRDINWKEQQIKLTDFPAQICQHELDHLEGILI"
     gene            complement(2606..3955)
                     /gene="gdhA"
                     /locus_tag="VBE22_001726"
     CDS             complement(2606..3955)
                     /gene="gdhA"
                     /locus_tag="VBE22_001726"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEO7203159.1"
                     /translation="MTSGKTYVASVLEKVKKQNEHEEEFLQAVEEVFESLVPVFDKYP
                     QYIEENLLERLVEPERVISFRVPWVDDKGQVQVNRGYRVQFSSAIGPYKGGLRFHPTV
                     TQSIVKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDNEVMRFTQSFMTELQKYIG
                     PDLDVPAGDIGVGGREIGYLYGQYKRLNGYQNGVLTGKGLTYGGSLARTEATGYGAVY
                     FAKEMLAACGQDLTGKVALVSGSGNVAIYATEKLQELGATVVAVSDSSGYVYDPDGID
                     LETLKQIKEVERARIVKYTEKHPKANFTPADQGSIWSIKADLAFPCATQNELDEEDAK
                     LLVENGVLAVTEGANMPSSLGAIKVFQKAGVAFGPAKAANAGGVAVSALEMAQNSSRR
                     AWTFEEVDQELQRIMKTIFVNASKAADEFGDSGNLVLGANIAGFLKVAQAMSAQGIV"
     gene            4123..4632
                     /locus_tag="VBE22_001727"
     CDS             4123..4632
                     /locus_tag="VBE22_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEO7203160.1"
                     /translation="MKWFPLVGGIIALLAGFYLFINPVTVVATLGWFIALFIFIYGIS
                     NLVRYLQADSYHRSPWYLVQSILAIIFGIILLSSSAMSLSNTVMTILAYWLLVSGILR
                     LLGGFQMQKINFPGAKSYKRSAYIALILGIILLVQPTLSAAIIGRFVAFILIAIGASA
                     IYTFTRINY"
     gene            complement(4673..>6159)
                     /locus_tag="VBE22_001728"
     CDS             complement(4673..>6159)
                     /locus_tag="VBE22_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7203161.1"
                     /translation="TTVVQKSIRDIRHDLNHKINKVPVSYFDKHQFGDMLGRFTSDVE
                     TVSNALQQSFLQIINAFLSIILVVVMVLYLNVPLAMIIIACIPVTYFSAQAILKRSQP
                     YFKEQAKILGELNGFVQEKLTGFNIIKLYGREEASSQEFRDITDNLRHVGFKASFISG
                     IMMPVLNGISDFIYLIIAFVGGLQVIAGTLTIGNMQAFVQYVWQISQPVQTITQLAGV
                     LQSAKSSLERIFEVLDEEEEANQVTEKLSHDLTGQVSFHGVDFHYSPDKPLIRDFNLD
                     VEPGQMIAIVGPTGAGKTTLINLLMRFYDVSEGAITVDGHDIRHLSRQDFRQQFGMVL
                     QDAWLYEGTIKENLRFGNLEASDEDIVAAAKAANVDHFIRTLPGGYNMVMNQESSNIS
                     LGQKQLLTIARALLADPKILILDEATSSVDTRLELLIQKAMKKLMEGRTSFVIAHRLS
                     TIQEADNILVLKDGQIIEQGNHQKLLADKGFYYELYNSQFSNSK"
BASE COUNT         1983 a         1210 c         1024 g         1942 t
ORIGIN      
        1 tttacaggag atttaaatga aaaagaaaat tattttgaaa agtagtgtcc ttggtttagt
       61 cgctgggact tctattatgt tctcaagcgt attcgctgac caagtcggcg tccaagttat
      121 aggcgtcaat gactttcatg gtgcacttga caatactgga acagcaaata tgcctgacgg
      181 aaaagttact aatgctggca ctgctgctca attagatgct tatatggatg atgctcaaaa
      241 agatttcaaa caaactaacc ctaatggtga aagcattaga gttcaagctg gtgatatggt
      301 tggagcaagt ccagctaact cagggcttct tcaagatgaa ccaaccgtta aaacatttaa
      361 tgcaatgaat gttgagtatg gcacattagg taaccatgaa tttgatgaag gtttggcaga
      421 atacaatcgt atcgttactg gaaaggcccc tgctccagat tctaatataa ataatattac
      481 gaaatcatac ccacacgaag ctgcaaaaca agaaattgta gtggcaaacg ttattgataa
      541 agttaacaaa caaatccctt acaattggaa accttacact attaaaaata ttcctgtaaa
      601 taacaaaagt gtgaacgttg gctttatcgg aatcgttacc aaagacatcc caaaccttgt
      661 cttacgtaaa aattatgaac aatatgaatt tttagatgaa gctgaaacaa tcgttaaata
      721 cgccaaagaa ttacaagcta aaaatgtcaa ggctattgta gtccttgctc atgtacctgc
      781 aacaagcaag gatgatattg ctgaaggtga agcagcagaa atgatgaaaa aagtcaatca
      841 actcttccct gaaaatagcg tagatattgt ctttgctgga cacaatcatc aatatacaaa
      901 tggtcttgtt ggtaaaactc gtattgtaca agcgctctct caaggaaaag cctatgctga
      961 tgtacgtggt gtcctagata ctgatacaca agatttcatt gaaacccctt cagctaaagt
     1021 aattgcagtt gctcctggta aaaaaacagg tagtgccgat attcaagcca ttgttgacca
     1081 agctaatact atcgttaaac aagtaacaga agctaaaatt ggtactgccg aggtaagtgg
     1141 catgattacg cgttctgttg atcaagataa tgttagtcct gtaggcagcc tcatcacaga
     1201 ggctcaacta gcaattgctc gaaaaagctg gccagatatc gattttgcca tgacaaataa
     1261 tggtggcatt cgtgctgact tactcatcaa accagatgga acaatcacct ggggagctgc
     1321 acaagcagtt caaccttttg gtaatatctt acaagtcgtc gaaattactg gtagagatct
     1381 ttataaagca ctcaacgaac aatacgacca aaaacaaaat ttcttccttc aaatagctgg
     1441 tctgcgatac acttacacag ataataaaga gggcggggaa gaaacaccat ttaaagttgt
     1501 aaaagcttat aaatcaaatg gtgaggaaat caatcctgat gcaaaataca aattagttat
     1561 caatgacttt ttattcggtg gtggtgatgg ctttgcaagc ttcagaaatg ccaaacttct
     1621 aggagccatt aatcccgata cagaggtatt tatggcctat atcactgatt tagaaaaagc
     1681 tggtaaaaaa gtgagcgttc caaataataa acctaaaatc tatgtcacta tgaagatggt
     1741 taatgaaact attacacaaa atgatggtac atatagcatt attaagaaac tttatttaga
     1801 tcgacaagga aatattgtag cacaagagat tgtatcagac actttaaacc aaacaaaatc
     1861 aaaatctaca aaaatcaacc ctgtaactac aattcacaaa aaacaattac accaatttac
     1921 agctattaac cctatgagaa attatggcaa accatcaaac tccactactg taaaatcaaa
     1981 acaattacca aaaacaaact ctgaatatgg acaatcattc cttatgtctg tctttggtgt
     2041 tggacttata ggaattgctt taaatacaaa gaaaaaacat atgaaataat aaaaataggt
     2101 ttggataaaa tatccaaacc tatttttata tcaatatgcc ttccaaatga tctaactcgt
     2161 gttgacaaat ttgagctgga aaatctgtca acttgatttg ctgttctttc caattgatat
     2221 cacgataaga aatcgtaatg tgatgatatc gttgtgtact tctgcaacct acgagagaaa
     2281 ggcaagattc ttctgtctca tatggatccg attttgcaac taatacaggg ttaaacatta
     2341 ctaaatttgt aatacccaca ttgatgataa tgacacgctt cagactacca atcatattgg
     2401 ctgccatacc tacacaataa ttttgattag catgcaaagt atcctgtaaa tcttttgcta
     2461 agctcacatc tgctctacta gccatttgtg atttttgttg taaaaagaaa gtatctctaa
     2521 caattggttt tatcatttat tttaacctaa ctctattatg ataatactag tataccatag
     2581 gttagctaag gactatgata agagattaaa caataccttg tgcagacata gcttgagcaa
     2641 cttttaaaaa gcctgcaatg ttagcaccta gaaccaggtt accactatcg ccaaattcat
     2701 cagccgcttt ggaagcattc acaaaaatag ttttcataat acgttgcaat tcttgatcaa
     2761 cttcttcaaa agtccatgcc ctacgactag aattttgtgc catttcaagc gctgaaacag
     2821 ctactcctcc tgcattggca gctttagcag gaccaaaagc aacccctgcc ttttggaaaa
     2881 ctttaatagc acccaagcta gatggcatat ttgctccttc tgttactgcc aagacaccat
     2941 tttcaactaa taacttagcg tcttcttcgt ctaattcgtt ttgagtagca cacgggaaag
     3001 ctaaatctgc tttaatagac cagatactac cttgatctgc tggtgtgaaa ttagcttttg
     3061 gatgtttctc cgtatattta acaatacgtg ctctttcaac ctcttttatc tgtttcaatg
     3121 tttcaaggtc aataccgtct ggatcataaa cataacctga agaatctgac acagctacaa
     3181 cagttgcacc caactcttgt aatttttcag tagcataaat agccacgtta ccagatcccg
     3241 aaaccagtgc aactttgcca gttaaatctt gaccacacgc tgccagcatt tctttagcaa
     3301 aatagactgc cccataacca gttgcttctg tacgagccaa agagccacca taagtcagtc
     3361 cttttccggt taaaacccca ttttgataac catttaaacg cttatattga ccataaagat
     3421 aaccaatttc acgtccacct actccaatat cgccagctgg cacatctaaa tctggtccaa
     3481 tgtatttttg aagttcggtc ataaaacttt gtgtgaaacg catcacttca ttatctgatt
     3541 tcccttttgg atcaaagttc gaccctcctt tgccaccacc aattggtaga cctgtcagtg
     3601 aattcttaaa aatttgttca aatcctaaaa atttgacaat agattgtgtt actgtagggt
     3661 gaaaacgtaa accaccttta taaggaccaa tagctgatga aaattgtaca cgatatcctc
     3721 ggttaacttg aacttggcct ttatcatcta cccatggaac tctaaaagaa atcacgcgct
     3781 caggctcaac caaacgttct aaaagatttt cttcaatgta ctgagggtat ttatcaaaaa
     3841 ctggtactag tgactcaaaa acctcttcaa ccgcttgtag aaattcttcc tcatgctcat
     3901 tttgcttctt cactttctct aaaacacttg ctacatatgt ctttccgctt gtcatttatt
     3961 ttcctccgta ttgttttaat atagttagca ttataaattg tcagaatttt cttgtcaact
     4021 atttgctatg gtatcattat taggagcaat tttaaaggta attatttgac ttttactgac
     4081 tttcgttcta taatgagaca aatatttgat tggagtatct atatgaaatg gttcccactt
     4141 gttggaggta taattgcctt attagctggc ttctatctct ttataaatcc tgtaacggtt
     4201 gttgcaactc tgggttggtt cattgcttta tttatcttta tttatggcat ttcaaattta
     4261 gtacgctact tgcaagctga ttcttatcac cgtagccctt ggtatctggt tcagagtatc
     4321 cttgctatta tttttggtat cattttacta tccagctcag caatgtcact ttcaaatact
     4381 gtcatgacta ttttagcata ctggctactg gtatctggta ttttacgact tttgggtggt
     4441 ttccaaatgc aaaaaattaa tttcccaggt gccaaatcct ataaacgttc ggcttatatt
     4501 gcactcattt taggtattat tttactggtt caacctacct tatcagcagc catcattgga
     4561 agatttgtag cctttatatt aatagcaatt ggagcttcgg ctatttacac ttttactcgt
     4621 attaattatt aaaaggacat gtttcgtgtc ctttttttat ctattgatga cgtcatttac
     4681 tatttgaaaa ttggctatta tataattcgt aataaaatcc tttatctgct aggagtttct
     4741 ggtgattacc ttgttcgatg atttgaccat cctttagaac taaaatgttg tctgcctctt
     4801 gaatcgttga tagacggtgt gcaatgacaa aactagttct tccttccatc aattttttca
     4861 tagctttttg aataagcaat tccaaccttg tatcaacaga tgaagtcgct tcatctaaaa
     4921 ttaaaatttt aggatctgct aataaagcac gtgcaatagt caacagttgc ttttgtccta
     4981 aggagatatt gcttgattct tgattcatca ccatattgta gccaccaggt aacgttcgaa
     5041 taaagtggtc aacattcgca gctttggcag cagcaactat atcctcatca ctggcttcaa
     5101 gattaccgaa tctaagattt tcttttattg taccttcata taaccaagca tcttgcaaca
     5161 ccatgccaaa ttgttgccgg aaatcttggc gtgataggtg tctaatatcg tgaccatcta
     5221 cggtgatagc accttcacta acatcgtaga atctcatgag taagtttata agtgttgtct
     5281 tacctgcacc tgttggccct acaattgcaa tcatttgccc tggttctacg tctaagttaa
     5341 agtcgcggat aagtggctta tccggagaat agtgaaaatc gacaccatga aagctgactt
     5401 gaccggttaa gtcatggctc aatttttcag tgacttgatt ggcttcctct tcttcatcta
     5461 agacttcaaa aatacgttct aaggaagatt ttgcactttg caaaacacct gctaattgtg
     5521 taatggtttg aacgggttga ctaatttgcc aaacatactg cacaaatgcc tgcatgttac
     5581 caattgttaa ggtaccagca ataacttgta aaccgccaac aaatgcaatg ataaggtaaa
     5641 taaaatcaga gataccattc aatactggca tcataattcc tgaaatgaag ctagctttga
     5701 aacctacatg acgtagatta tccgttatat cacgaaattc ttgactagac gcctcttctc
     5761 taccatataa ttttatgata ttgaaaccag ttaatttctc ttgtacaaaa ccattcagtt
     5821 ctcccagaat ctttgcctgt tctttaaagt aaggttggga tctttttagt atcgcttgtg
     5881 cactaaaata agttacagga atacaagcaa tgataatcat ggctaatggt acatttagat
     5941 aaagtaccat aacgactact aaaataatac ttaaaaaagc attgataatc tgtaaaaagc
     6001 tctgttgtaa agcatttgag accgtttcaa catcacttgt aaaacgtccc aacatatctc
     6061 caaattggtg cttatcaaaa tacgatacag gaactttatt gattttatga ttcaaatcat
     6121 gacggatatc acggatactt ttttgaacga cagtagtca
//
LOCUS       DATRTN010000094         5937 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.094, whole genome shotgun sequence.
ACCESSION   DATRTN010000094 DATRTN010000000
VERSION     DATRTN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.094"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            45..1544
                     /locus_tag="VBE22_001729"
     CDS             45..1544
                     /locus_tag="VBE22_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO7203162.1"
                     /translation="MTTKAKHDIHGKPYNRTAMITLLLIATFAGVLNQTSLGTAIPTL
                     MNSFNISLSTAQQATTWFLLANGIMIPVSAYLATRFSTKWLYVTSYVVLLIGLLMTTL
                     APTSNWNLFLVGRIIQAISVGISMPLMQVVMVNVFPPEQRGAAMGLNGLVVGLAPAIG
                     PTLAGWILKQEFHFAGHDLTWRAIFLLPLLILTVTTILSPFVLKDVVDNKSVKLEVPS
                     LILSIIGFGSFLWGFTNVATYGWGDIGYVISPIMVGIIFIALFIHRQLKLETPFLDIR
                     VFKNKQFSVTTAAIALSMMAMMGVEMMLPLYLQNVHGLSALDSGLALLPGALMMGIVS
                     PISGAVYDKVGARRMAMIGFTILGVATLPFVFLTTTTPDHFITLLYAVRMFGIAMVMM
                     PLTASAMSALPPHEAAHGTAANNTARQIASAVVVALLSSVAQNIITNNKPSKDLLTMN
                     PLKYANQMLNASLDGFHVSFAIGFVFAVLGLLVSLFLRKGKIIETEKEV"
     gene            1546..2262
                     /locus_tag="VBE22_001730"
     CDS             1546..2262
                     /locus_tag="VBE22_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEO7203163.1"
                     /translation="MIILIIALATILTFVTWMLIRPKALRYILGLLSLALLFASVWML
                     TDHFVNHTGMTIETKTKTREIYSAGDSNAPFGLMIYKEIGTHSSNNVLVYRTDLNQKE
                     AKAHFVPNTKEVTSAVKKSASYQLADVKKATVTTKTKRYVWKDSLSKLLYGFAGENGE
                     LISQKSVAKIPRKTWLVLTKEQSEHLESAAAKLKEQMTLQAKVNPVKAMEIKTLAKTN
                     PEAYAEMQVKALKEALNIKE"
     gene            2362..3429
                     /locus_tag="VBE22_001731"
     CDS             2362..3429
                     /locus_tag="VBE22_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO7203164.1"
                     /translation="MGFEQRLTRCQTAISQLSCDALLITNLTNIFYLTGFSGTNATVL
                     ISPKHRIFVTDSRYALIAKNTVREFDIIISREPLAAILKIIRDDALMAIGFETDISYH
                     MYKHMVEVFEDYRLIEAPSVVEKLRMIKDATELAIIRQACRISDQAFLDVLDFIKPDR
                     TTELQVANFLDFRMRELGATGPSFDFIVASGYRSAMPHGVASQKTIQSGEALTLDFGC
                     YYQHYVSDMTRTIHIGHVTDQEREIYDIVLKSNQAIIDNVKSGMKRCDYDYLARQVIE
                     NSGYGNHFTHGIGHGMGLDVHEIPYFGKSEGVIASGMVVTDEPGIYLDNKYGVRIEDD
                     LLITETGCEVLTSAPKELIVL"
     gene            3441..3893
                     /locus_tag="VBE22_001732"
     CDS             3441..3893
                     /locus_tag="VBE22_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEO7203165.1"
                     /translation="MNRLSWEDYFMANAELISKRSTCDRAFVGAVLVKNNRIIATGYN
                     GGVSETDNCNEVGHYMEDGHCIRTVHAEMNALIQCAKEGISTNNTEIYVTHFPCINCT
                     KALLQAGVKKITYKANYRPHPFAIELMEAKGVAYVQHDVPEVTLGMEE"
     gene            3941..4315
                     /locus_tag="VBE22_001733"
     CDS             3941..4315
                     /locus_tag="VBE22_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEO7203166.1"
                     /translation="MHRSFLEENYLEAIYILQQRNESVRSIDVAEYLSVAKSSVNSAV
                     NKLLTQQYITFEDDKIIRMTAEGEKIAEFVYEKHLYLKKLLCKMGIDETEADEIACLL
                     EHILKEDSFKKIKKFVQDKKII"
     gene            4532..5347
                     /locus_tag="VBE22_001734"
     CDS             4532..5347
                     /locus_tag="VBE22_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO7203167.1"
                     /translation="MIKNGDSIAVVNGDIELSYKDIDRQSNSFANGLLKAGFKCGDKI
                     VLQLPNYHEFIIIAFAMFKVGIIPIMSLPAHRKNELKGIIEKSEAVAYIAKDKYLGFS
                     YVDMIRDIKIESDREFKVYILGEGDEFKKFSELNDNNYIYQKTEVDYKNIGLLLLSGG
                     TTRIPKLIPRRHCDYLYVAKKTSIISGMDRGSIYLAALPMAHNFPLCCPGILGTFAVG
                     GKVVVCEVTSPDEILPLIEEEKVTITGFVPAIANICMDYLQYEDYDLSSLKIL"
     gene            5447..5890
                     /locus_tag="VBE22_001735"
     CDS             5447..5890
                     /locus_tag="VBE22_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203168.1"
                     /translation="MTTSIDDDEKTVWQTQGKPISEPDEILIVDKARKLKDGNYQIAG
                     RIKEIINRAGEKITPLELEEILLTHDCINSVQVLGVPDKLHGEAIAVFILKSESKRLT
                     LEEVRKFLISNNVADFKLPDAVKYIDSWPLTALGKIDRNKLKENF"
BASE COUNT         1980 a          890 c         1212 g         1855 t
ORIGIN      
        1 ttaaaagata ttagttaaaa tcagaaatag agattggaga taaaatgact acaaaagcaa
       61 aacatgatat tcatggcaaa ccatataatc gtacagccat gatcacctta ttgttgattg
      121 ctacatttgc aggggtgtta aatcagacaa gtttgggaac ggctatccca actttgatga
      181 atagctttaa catttcgttg tcaacggctc aacaagcaac aacatggttt ttattagcga
      241 atgggattat gattcctgtt tcagcttatt tagcgacacg tttttcaaca aaatggttgt
      301 atgtgacatc ctacgttgtt ttacttattg gactgttaat gacgacgtta gcacctacat
      361 ctaactggaa ccttttttta gtaggacgta ttattcaagc gatttctgta gggatttcta
      421 tgcctttgat gcaggttgtt atggtgaatg tttttcctcc agagcagcgt ggtgccgcta
      481 tgggacttaa tggtttggtg gttggtcttg ccccagctat tgggcctaca ttagcagggt
      541 ggattttaaa acaagaattt cattttgcag ggcatgattt gacatggcgt gcaattttcc
      601 ttcttccttt actaatttta acggtcacta caattttatc cccctttgtt ctaaaagatg
      661 tggttgataa taagtcagtt aaattggaag tgccttccct tattctttca ataattggtt
      721 ttggtagttt tttgtggggc ttcacaaatg tggcaactta tggatgggga gatattggat
      781 atgttatttc tcctattatg gttggtatta tttttatcgc cttattcatt catcgtcaat
      841 taaaactaga aacaccgttc ttagatatcc gtgttttcaa aaataaacaa ttctcagtaa
      901 caacagcagc tattgcactt tcaatgatgg cgatgatggg tgttgaaatg atgctaccgc
      961 tttacttgca aaatgttcat ggtctctctg cacttgattc tggtttagct ttattaccag
     1021 gtgccttgat gatggggata gttagtccaa tttctggagc tgtatatgat aaagttggtg
     1081 caagacgtat ggctatgatt ggttttacca tactaggtgt agcgacgtta ccttttgttt
     1141 tcttaacgac tacaacacca gatcatttta ttaccctctt atatgcagta cgtatgtttg
     1201 gtattgctat ggttatgatg ccattgacag caagtgcgat gagcgcactt ccaccacatg
     1261 aggcagcaca tggaacagca gctaataata ctgctcgtca aattgcttca gcagttgtag
     1321 ttgcattgct ttcaagtgtt gctcaaaata ttatcaccaa caataaacca tcaaaagatt
     1381 tgctaacaat gaatccttta aaatatgcaa atcagatgtt gaatgctagt cttgatggct
     1441 tccatgtttc ttttgccatt ggttttgtat ttgcggttct tggtttactg gtatctcttt
     1501 tcctgagaaa agggaaaatt attgagacgg aaaaggaggt atagcatgat tattttgatt
     1561 attgcactgg caactatttt gacatttgtc acatggatgt tgattcgtcc aaaagctcta
     1621 cggtatattc taggtcttct ctcattagct ttgttatttg cctcagtttg gatgttaacg
     1681 gatcactttg ttaatcatac aggtatgact attgaaacta agacaaagac acgtgagatt
     1741 tatagtgcgg gtgatagtaa cgctccattt ggtctcatga tttataaaga gattggtact
     1801 cattcgagta ataatgtttt agtttatcga acagatttaa atcaaaaaga agcaaaagct
     1861 cactttgttc caaatacaaa agaagtgaca agtgctgtga aaaaatctgc tagctaccaa
     1921 ttagctgatg tgaaaaaagc cacagtaaca acaaagacta aacgttatgt gtggaaagat
     1981 agtctttcga aactacttta tggctttgca ggtgaaaatg gagaattgat ttctcagaaa
     2041 tcagttgcta aaataccacg aaaaacttgg ctagttttga ctaaagagca gtccgaacac
     2101 ttagaatcag ctgcggctaa actaaaagaa cagatgactt tgcaagcaaa agtaaaccca
     2161 gtgaaagcta tggagataaa gacattagct aagacaaatc cagaagctta tgctgagatg
     2221 caagttaaag cattaaaaga agctcttaat ataaaagaat agtaaagtaa aagcaactga
     2281 aatgagttgc ttttcttata gctaaatgtc ttaaaagatg ataaaattaa tctaattaag
     2341 gttagaatag cgaggtttct tatgggtttt gaacaacgtc taactagatg tcaaacagca
     2401 atttcacaat tatcatgtga cgcactctta ataacgaatt taacaaatat tttttatttg
     2461 actggttttt caggaactaa tgcaacggta ctcattagtc caaaacaccg gatttttgtt
     2521 acagattcac gatatgcctt gatagcaaag aatactgtaa gagagtttga tattatcatt
     2581 agcagagaac cacttgcggc tattttgaaa attattcgag atgacgcctt gatggcgatt
     2641 ggttttgaaa ctgatatctc ttatcatatg tacaaacata tggtggaagt ttttgaagat
     2701 tatcgcttga tagaagctcc atctgttgtt gaaaagttac gtatgattaa agatgcaaca
     2761 gaacttgcga tcatccgtca agcctgtcgt atttcggacc aagctttttt agatgtgttg
     2821 gattttataa agcctgatcg aacaactgaa ttgcaagttg ctaatttttt agactttcgc
     2881 atgagagaat taggagcaac tggtccttca tttgatttta ttgtagcatc tggatatcga
     2941 tcagcgatgc cacatggggt tgcgagtcag aagactattc aatcgggaga agctctgact
     3001 ttagattttg gttgttacta tcagcactat gttagtgata tgacacgtac gattcatatc
     3061 ggtcatgtca ctgatcagga acgcgagatt tacgatatcg ttttgaaaag taatcaagct
     3121 attattgata atgtaaaatc aggaatgaag cgttgtgatt atgattactt ggcgcgacaa
     3181 gtcattgaaa attctggata cggtaatcat tttacccacg gtatagggca tggtatgggg
     3241 cttgatgttc atgaaattcc ttattttggt aagtcagagg gagtgattgc atctgggatg
     3301 gtagtgactg acgagccagg aatatatctc gataataaat acggtgttcg tattgaagat
     3361 gatttattaa taacggaaac aggttgcgaa gtattgacat ctgcacctaa agaattaatt
     3421 gtactttaaa ggaaaataga atgaatagac tttcttggga agattatttt atggcaaatg
     3481 cagagttgat ttctaaacgt tctacctgtg accgtgcttt tgttggagct gttttagtca
     3541 aaaataatcg catcattgct acaggttata acggaggagt ctctgagaca gataactgta
     3601 acgaagtggg acattatatg gaagatggtc actgtattag gactgttcat gctgagatga
     3661 atgcccttat tcagtgtgct aaagaaggta tttctactaa taatacagaa atttatgtga
     3721 cgcattttcc ttgtatcaat tgcaccaagg cgctcttgca agccggggtt aagaaaatta
     3781 cttataaagc taattatcgc ccacacccat ttgctattga acttatggaa gcaaaaggag
     3841 ttgcttatgt tcaacatgat gttccagagg taacactagg gatggaagag taaagaggaa
     3901 gatagagcat atataaaatt tattaagagg aagtgactta atgcatagat cttttttaga
     3961 agaaaattac ttggaagcga tttatatctt acaacaaaga aatgaaagtg ttagaagtat
     4021 tgatgtagcc gaatacttgt ctgttgctaa atcttcggtg aactcagcag taaataaatt
     4081 actcactcag caatatataa cctttgaaga tgataagatt ataaggatga ccgctgaggg
     4141 agaaaaaata gcggaatttg tttatgaaaa acatctatat ttaaaaaaat tactatgtaa
     4201 gatgggaatt gacgaaacag aagcagatga aatagcttgt cttctagaac acattttaaa
     4261 agaagacagc tttaaaaaaa taaaaaaatt tgtccaagac aaaaaaatta tttaagaaaa
     4321 gtaaagttga aatatcaggt tagaaagtac atctaaacta ccttatctgt ggataattta
     4381 aaaacaaaca aagaaaagta ttgatatttg ttctcagata tgtacaatat agttagatag
     4441 aactaaccgg atattttttt gccctttagg ttagatgcac ctaactagaa aattcaaaga
     4501 ggaaatttga tggaaacata tattgttaac tatgataaaa aatggtgata gcatagctgt
     4561 tgtaaacgga gacatagagt tgtcttacaa ggatattgat aggcagtcaa actcttttgc
     4621 caatggactt ttaaaagctg gcttcaaatg tggagataaa atagttttac aattaccgaa
     4681 ttatcacgag tttataataa ttgctttcgc catgtttaaa gtaggcatta tcccgattat
     4741 gagtttgcct gctcatagaa aaaacgaact aaaagggata atagaaaaat cggaagctgt
     4801 ggcatacata gcaaaagata agtacctagg cttttcttat gttgatatga tacgagatat
     4861 aaagatagag tcagacagag aatttaaagt ttatatactt ggtgaaggag atgaatttaa
     4921 aaaattttct gaattgaatg ataataatta tatctatcaa aaaaccgagg ttgattataa
     4981 aaacatagga ctacttcttt tatccggagg aacaaccaga ataccaaagc tgattccgag
     5041 aaggcactgt gattatctgt atgtagcaaa gaaaacatct attataagcg gtatggatag
     5101 aggcagtata tacttagcag ccttgccgat ggcacataat tttcctttgt gttgccccgg
     5161 cattctcgga acttttgctg taggtgggaa agttgttgta tgtgaggtaa ccagtcctga
     5221 tgaaatacta cctctgatag aggaagaaaa ggttacaata acgggttttg tgccagccat
     5281 cgcaaatatt tgcatggatt atttacagta tgaagattat gatctttcat cattaaagat
     5341 attgtaaatc ggtgggtctg ttttagagcc ttggttagcg gaaaagattg aagaaacatt
     5401 taatgtaaag ttacaacaaa tattcggtat agctgaaggt cttattttga caacgagcat
     5461 agatgatgac gaaaaaaccg tatggcaaac acagggtaag ccaattagcg aacctgatga
     5521 aatactcatt gtggataaag cgagaaaatt aaaagatggc aattatcaaa ttgccggaag
     5581 aattaaagag ataatcaaca gagcgggcga aaagattaca ccactcgaat tggaagaaat
     5641 cttattaaca catgattgca taaattccgt tcaagtttta ggagtgcctg ataaattaca
     5701 tggcgaagca attgccgtat tcattttaaa aagcgaaagc aagaggctta cacttgaaga
     5761 agtaaggaaa tttttaatat caaataacgt agcagacttc aaactacctg atgcggttaa
     5821 gtatatagat agttggccgt taacagcgtt aggcaaaata gatagaaaca aattaaaaga
     5881 aaatttttag aggagaactt gaaatggaac ttaacggtgt aaaagaaaat gccaaaa
//
LOCUS       DATRTN010000095         5692 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.095, whole genome shotgun sequence.
ACCESSION   DATRTN010000095 DATRTN010000000
VERSION     DATRTN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.095"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(195..1391)
                     /locus_tag="VBE22_001736"
     CDS             complement(195..1391)
                     /locus_tag="VBE22_001736"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEO7203169.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILEQDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRNTIAEIGYDKAEYGFSAESVGVHPSLVEQSP
                     DIAQGVNEALEVRGSLEQDPLDLIGAGDQGLMFGFAVDETPELMPLPISLAHQLVKKL
                     TDLRKSGELTYLRPDAKSQVTVEYDENNQPIRVDAVVISTQHDPNVTNDQLHKDVIEK
                     VINEVIPSHYLDDQTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKVEVQLAYAIGVAQPVSVRVDTFGTGVIA
                     EADLEAAVRQIFDLRPAGIINMLDLKRPIYRQTAAYGHMGRTDIDLPWERVDKVQALK
                     DFIASK"
     gene            1576..2511
                     /locus_tag="VBE22_001737"
     CDS             1576..2511
                     /locus_tag="VBE22_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEO7203170.1"
                     /translation="MKTYEKIYQILSKNNNFISGETMANQLNISRTAIWKGIKTLEEL
                     GLEIESVTNKGYRLVSGDILLPEQLEQEIGIKVSLNNNSASTQLDAKMGIESKLKTPH
                     LFLAPNQKKAKGRFDRPFFTSNQGGIYMSLLLQPNVPIEDIKPYTVMVASSAVKAISR
                     LTGITPEIKWVNDIYLDNKKIAGILTEAIASVESGLVTNVIIGLGINFYIKEFPRALT
                     KRAGSLFTEQPTITRNQLITEIWNLFFNIPLEDHLKVYREKSLVLDKTVSFMDGQTMY
                     SGKAIDITDKGYLVVELDDGQLKTLRSGEISLSSW"
     gene            complement(2492..2686)
                     /locus_tag="VBE22_001738"
     CDS             complement(2492..2686)
                     /locus_tag="VBE22_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEO7203171.1"
                     /translation="MTKRQFIFMALLCSFETYFFNQSVMDGSWIFAIFWGVLLLRDLQ
                     KVYAISKFTKELIKSTKKKD"
     gene            complement(2775..4439)
                     /gene="dnaX"
                     /locus_tag="VBE22_001739"
     CDS             complement(2775..4439)
                     /gene="dnaX"
                     /locus_tag="VBE22_001739"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEO7203172.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVSSKKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQINGEPCNHCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKLLAIRDHLAQILDKEAISYDLDALTLVARRAEGGMRDALS
                     ILDQALSLAKDNHISLDVAEEITGSISLSAIDDYVSNILAHDTTEALAKLEVIFDSGK
                     SMSRFATDLLMYLRDLLVVQAGGEDSHSSDTFIANLNVKQDILFEMIDKVTSVLPEIK
                     NGSHPKVYAEMMTIQLSEMVEKNSSNIPADVTAELDSLRRELKSLKNEMSQLSRADQS
                     SSTQKVKVNNKTFTFKVDRTKILTIMEETVVDSQRSREYLEALKSAWNEILDNITAQD
                     RALLMGSEPVLANSENAILAFDAAFNAEQAMKRTDLNDIFGNIMSKAAGFSPNILAVP
                     RNDFNQIRSDFAKKMKAQKTETEPEVNHQIPEDFSYLAERIAIVED"
     gene            complement(4439..4936)
                     /locus_tag="VBE22_001740"
     CDS             complement(4439..4936)
                     /locus_tag="VBE22_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEO7203173.1"
                     /translation="MNKSKKIENYQLLLLQAQALFSDETNALANLSNASAMLNAMLPN
                     SVFTGFYLFDGKELILGPFQGGVSCVHITLGKGVCGESAQTAKTLIVDDVTKHANYIS
                     CDSKAMSEIVVPMFKNGKLLGVLDLDSSLVADYDEIDQEYLEKFVGILVEHTIWNLDM
                     FGVEK"
     gene            complement(5023..5652)
                     /gene="udk"
                     /locus_tag="VBE22_001741"
     CDS             complement(5023..5652)
                     /gene="udk"
                     /locus_tag="VBE22_001741"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEO7203174.1"
                     /translation="MRKKPIIIGVTGGSGGGKTSVSRAILSNFPDQKITMIEHDSYYK
                     DQSHLTFEERVKTNYDHPLAFDTNLMIEQLNELIEGRPVDIPVYDYTKHTRSDRTIRQ
                     EPQDVIIVEGILVLEDQRLRDLMDIKLFVDTDDDIRIIRRIKRDMEERDRSLDSIIEQ
                     YTEVVKPMYHQFIEPTKRYADIVIPEGVSNIVAIDLINTKVASILNEAK"
BASE COUNT         1848 a         1097 c          900 g         1847 t
ORIGIN      
        1 tgaaattatt ttttttgaaa aatttttcga taggaaaatt caattaactt ggtcatcatt
       61 ttattcacaa tatacaccat attttcaaga aatatgcagt atttcctata cttaaccagt
      121 ttattataag attttataaa tgttctataa attatcatga gcaaaaaagc gtaaactttc
      181 gtttacgctt tttattattt gctagcaata aaatccttta aggcctggac tttatcaact
      241 cgctcccatg gcaaatcgat atcagtacgt cccatatgcc cataggctgc tgtttgtcga
      301 taaataggtc gtttgaggtc caacatatta ataatgcctg ctggtcgcaa atcaaatatt
      361 tgacgtacag ctgcctctag gtctgcttct gctataacac ctgttccaaa tgtatctaca
      421 cgcacagaga caggctgggc aacaccaatt gcataagcaa gttgtacttc tactttttta
      481 gctaaatcag ctgcaacaat attttttgca atataacgag ctgcataaga tgctgaacga
      541 tctactttag tagcatcttt acctgaaaat gcaccaccac catgacgaga ataaccacca
      601 taagtatcta caataatctt acgacctgtt agtcctgaat ctccttgagg gcctccaata
      661 acaaagcgcc ctgtaggatt gataaagaat tttgtttggt catctaaata atggcttgga
      721 ataacttcat tgataacttt ctcaatcacg tccttatgga gttggtcatt agttacatta
      781 ggatcatgct gtgtcgaaat aactactgca tccacacgaa ttggttgatt attttcatca
      841 tattcaaccg ttacttgaga ttttgcatct ggtcgaagat aggtgagttc cccagactta
      901 cgtagatctg tcaacttctt aactaactga tgagctaaag aaattggcaa tggcattaac
      961 tcaggtgttt catcaacagc aaacccaaac atcaaacctt ggtcacctgc accaattaaa
     1021 tctaaaggat cttgttcaag agaaccacgt acctcaagag cttcattaac tccctgtgca
     1081 atgtctggcg actgttcaac gagtgacgga tgcactccga cagattcagc tgagaagcca
     1141 tattcagctt tatcatagcc aatttcagcg attgtgttac ggacaactcg gttaatatca
     1201 acataggctg tcgttgatat ctcgccaaat acatgaaccg accctgtata aacagctgtt
     1261 tcggctgcta cgtgggcatc tggatcttgc tctaaaattg catctaaaat cgcatcggaa
     1321 atttggtcag caatcttatc gggatgtccc tcagatactg actcagacgt gaaaagttta
     1381 cgttctgaca taaaaatgtc ccccctaaaa tgtaaaatgt gtcgagttac aaagaactag
     1441 cctctaggct accttttcgg gactcatata gctattgtac cactatttca tcttttgtga
     1501 aataagttac caataaataa aaaattatct ctattttctt tagtcttttt tctcgattta
     1561 tactatacta gatttatgaa aacctacgaa aaaatttatc aaatactatc aaaaaataac
     1621 aactttatta gcggagaaac tatggcaaat caacttaata tttcccgcac agctatttgg
     1681 aaaggtataa aaacgctaga agaattaggc ctagagattg agtctgtcac taacaagggc
     1741 taccgattgg tatcaggtga catattatta ccagaacaac tcgaacaaga aattggtatt
     1801 aaggttagtc ttaataataa tagtgcttca acccaactag atgctaaaat ggggatagaa
     1861 agcaaattaa agactccaca ccttttttta gctcctaatc aaaaaaaagc caaaggacgt
     1921 tttgatagac ctttctttac atcaaatcaa ggcggcatct atatgtctct tttattacaa
     1981 cctaatgtac cgattgaaga tataaaacct tatactgtca tggtagcctc tagtgctgta
     2041 aaagctatct cacggttaac aggaattact cctgaaatta agtgggtcaa tgatatttat
     2101 ttagataata aaaaaatagc tggtatttta accgaggcta tcgcttctgt tgagagtgga
     2161 ttggtaacga atgttattat aggattaggt atcaatttct atatcaaaga gttccctagg
     2221 gcgttaacta agcgcgcagg tagtctcttt acagagcaac caaccataac tagaaatcaa
     2281 ttgattacag aaatctggaa tctctttttc aatattccac tagaggatca cttaaaggta
     2341 tatagagaaa aatccctagt cttagataaa accgttagct ttatggatgg tcaaacaatg
     2401 tattctggga aagctattga tattactgat aaaggatatt tagtggttga gttagatgat
     2461 ggtcagttga aaacacttcg cagtggcgaa atcagtcttt cttcttggta gatttgataa
     2521 gctcttttgt gaattttgaa atcgcataca ccttttgaag atctcgaagt aataaaactc
     2581 cccaaaaaat agcaaaaatc caggagccat ccataacgga ttgattgaaa aaataagttt
     2641 caaaagagca taaaagcgcc ataaaaataa attgtcgttt tgtcatatca ccattataac
     2701 agaatctact aaataattaa aaaacttatc aaaaaaatag ttgcctgtta caggcaacta
     2761 tttttagtta cgttttaatc ttcaacgatt gctattcgtt ctgctagata agaaaagtct
     2821 tctggaatct gatgattaac ttcaggttct gtttccgtct tttgagcttt cattttcttt
     2881 gcaaaatcgc tccgaatttg attaaaatca tttctaggta cagctaaaat attaggtgaa
     2941 aaacctgctg ctttactcat aatattacca aatatatcat tgagatcggt acgtttcata
     3001 gcttgttcag cattaaaggc cgcatcaaat gccaaaatag cattctcgct attagctaag
     3061 acaggttcag atcccattaa taaggctcta tcttgagccg taatattatc taatatctcg
     3121 ttccaagcac tctttaaagc ttctaaatat tcacgtgaac gttggctatc tacaaccgtt
     3181 tcttccataa tggtcaaaat ctttgtacgg tcaactttga atgtgaatgt cttattatta
     3241 acctttactt tttgagttga tgaactttga tctgctcgag agagttggct catttcgttt
     3301 tttaacgatt ttaactctcg acgaagacta tctaattctg ctgtcacatc tgcgggtatg
     3361 tttgacgaat ttttttctac catttcagac aattggatgg tcatcatctc tgcataaact
     3421 ttaggatggc taccattttt tatctcagga agaacagatg tcactttatc aatcatttca
     3481 aataaaatat cttgtttaac attaagattg gctataaaag tatcacttga atgactatct
     3541 tcaccacctg cttgaacaac aagtaaatct cttaagtaca tcaataaatc tgttgcaaaa
     3601 cgactcatac ttttgccact atcaaagatt acttcaagtt tagcgagtgc ttcagttgta
     3661 tcgtgagcca atatattcga tacataatca tctatagcag ataaggaaat gctccctgta
     3721 atttcttctg ctacatctaa agaaatgtgg ttatctttcg ctaaactcaa ggcttgatcg
     3781 agaattgata aagcatcacg cataccacct tcggctcgtc tagcgactag tgtcagagca
     3841 tccaaatcat atgaaatagc ttctttatct aaaatctgtg ctaaatgatc tcgaatagct
     3901 aataatttaa tcgctttaaa ttcaaagcgc tggacacgag ataagattgt tgcagggatc
     3961 ttatggagtt ctgttgttgc taatataaaa acaacatttt ctgttggctc ttcaagtgtt
     4021 ttcaatagtg cattgaaggc acctgtagac aacatatgaa cctcatcaat aatataaact
     4081 ttataggttg ccctacttgg agcataagtt gatttgtccc tgatatcacg aatttcatcg
     4141 acaccattat ttgacgcagc atcaatttca atgacatcct ctaaactacc atttgtaata
     4201 tctcgacaaa tatcacaatg attacaaggt tcgccattaa tttgatttgg gcaattcatc
     4261 gcttttgcaa atattttagc agcacttgtt tttccagttc ccctaggtcc tgaaaaaagg
     4321 taagcatggc taattttttt gctggacaca gcttgcttta aagtagttga aataacactt
     4381 tgtccaacca tttcatcaaa tgtttggcta cgatatttcc gataaagggc ctgatacatt
     4441 acttttcaac tccaaacata tccaaattcc aaatcgtatg ttctactaga atacctacaa
     4501 atttttctaa gtattcttga tcaatctcat cataatctgc tactaaagaa gaatctaaat
     4561 ctagaactcc tagaagtttg ccatttttaa acataggtac tacgatttca ctcatagctt
     4621 ttgaatcaca ggagatatag ttagcatgct ttgtaacatc atcaacaatc agcgtcttag
     4681 cagtttgtgc agattcacca caaacacctt ttcctaaagt aatatgcaca catgatacac
     4741 caccctggaa agggccaaga attaactcct ttccatcaaa taaataaaag cctgtaaata
     4801 cagaatttgg aagcatagca tttagcatag ctgaagcatt tgataagttg gcaagagcat
     4861 ttgtttcatc tgagaataga gcttgcgctt gtagtaataa taattgataa ttttcgattt
     4921 tcttagactt gttcatagca cttattatag cataatttga aagttactaa aactaaataa
     4981 ccatcctata taaaacaaaa aaataaacac aaaccattgt gtttattttg cttcatttaa
     5041 gatgctagcc actttggtat tgattaaatc aatagcgaca atattactta ctccttctgg
     5101 aattacaata tccgcataac gctttgttgg ttcgataaat tgatgataca taggttttac
     5161 aacttctgta tactgttcaa taatactatc taaacttcta tcgcgctctt ccatatcacg
     5221 cttaatacga cgaataatac gaatatcatc atctgtatca acaaataatt tgatatccat
     5281 taaatcacgc aaacgctgat cttctaatac taaaattccc tcaacaataa tgacatcctg
     5341 gggttcttgg cggatagttc tatcactacg ggtatgtttt gtgtaatcat aaaccggaat
     5401 atcgaccgga cgtccctcaa tcaattcgtt caattgctca atcatcaaat tggtatcaaa
     5461 agcaagggga tgatcataat tggtttttac acgttcttca aaagtcaaat gactttgatc
     5521 tttatagtaa gaatcatgtt caatcatggt aattttttga tctgggaaat ttgataaaat
     5581 cgcccgagaa acactggttt taccaccacc agatccacct gtaacaccaa taataatagg
     5641 ttttttacgc atttgtcact ccaaattaaa tttatcctct atctattgta ct
//
LOCUS       DATRTN010000096         5656 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.096, whole genome shotgun sequence.
ACCESSION   DATRTN010000096 DATRTN010000000
VERSION     DATRTN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.096"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..860)
                     /locus_tag="VBE22_001742"
     CDS             complement(<1..860)
                     /locus_tag="VBE22_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7203175.1"
                     /translation="MLDILSEKHLERRQRIIISAIAIALAAQINISILADGFIMTLSL
                     FILPVFLYFNDDINPILLCLGITFASPIFRGIILSIAGEAEIHQIIEFVLTDMAFYIC
                     YGITFYTIYWHRSYRNKGTFFFSIIICDYFANLVEISFLIKFNNYTITIFATLFAIAL
                     LRAFISCAVAYTYSYLSLLLQKDDHERRYYYFMWSTSAVKSEVYFMQKNIIEIENIMK
                     NAYLLDKELSKYHLPKEYQHLSLDISRDVHEVKKDYQNIIKGLGTYFSVKNESTMALK
                     DIFQIVLSYT"
     gene            963..1961
                     /gene="argF"
                     /locus_tag="VBE22_001743"
     CDS             963..1961
                     /gene="argF"
                     /locus_tag="VBE22_001743"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO7203176.1"
                     /translation="MKNLRNRSFLTLLDFSTAEVEFLLKLSEDLKRAKYAGIEQQKLV
                     GKNIALIFEKDSTRTRCAFEVAAHDQGAHVTYLGPTGSQMGKKETSKDTARVLGGMYD
                     GIEYRGFSQETVETLAEFSGVPVWNGLTDADHPTQVLADFLTAKECLHKPYKDIRFTY
                     VGDGRNNVANALMIGASIVGMTYHLVCPKELEPDPELLSKCQEIAKTTGASIEITADI
                     AEGVRDSDVLYTDVWVSMGEPDEVWKERIALLEPYRITQEMLNMTENPNVIFEHCLPS
                     FHNIDTKVGYDIYEKYGLKEMEVSDEVFEGPHSVVFQEAENRMHTIKAVMVATLGD"
     gene            1973..2899
                     /gene="arcC"
                     /locus_tag="VBE22_001744"
     CDS             1973..2899
                     /gene="arcC"
                     /locus_tag="VBE22_001744"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO7203177.1"
                     /translation="MAKIVVALGGNALGNSPEEQLRLVKHTAKSLVALIKKGHEIVVS
                     HGNGPQVGAINLGMNFAAESGQGTNFPFPECGAMSQGYIGYHLQQSLLNELRQEGINK
                     EVATIITQIEVDESDQAFSAPTKPIGTFYDKETSEKIAIEKGYTFVEDAGRGYRRVVA
                     SPEPKKIIEINSIKTLIENDTLVIAGGGGGIPVINKGGYEGIAAVIDKDKSSALLAGE
                     LAADQLIILTAVDYVYTQFGKENQKALTEVNENQMIDYVNQGEFAKGSMLPKVIACMS
                     FLDHNPKGTALITSLNGLEDALDGKLGTRITK"
     gene            2993..4546
                     /locus_tag="VBE22_001745"
     CDS             2993..4546
                     /locus_tag="VBE22_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEO7203178.1"
                     /translation="MPEKMTNSENKRSKGFRMPGAFTILFILTIFSVLATWWIPAGSY
                     SKLQFDTASSKLVVTDPNGKTVHVPATQTQLDKMNVKIKIKEFTSGAISKPVSVPNTY
                     KRLKQNPAGIGSVTTSMVNGTIEAVDIMVFIMVLGGMIGVVRKSGAFESGLLALTKKT
                     KGREFLLIFLVSLLMVLGGTLCGIEEEAVAFYPILVPIFLAMGYDSIICVGAIFLASS
                     VGTSFSTINPFSSVIASNAAGISFTEGLSWRTAGCIAGAIFVVVYLHWYAKKIKANPE
                     FSYSYEDRVEFNAKWGMTTNHTPSLFTIRQKIILSLFVISFPLMVWGVMSQGWWFPTM
                     ASSFLAITIIIMFLTATGANGIGERDVVDEFVNGASSLVGVSLIIGLARGINIILSQG
                     YISDTMLYTASKLASHVSGSVFIIVMMFIYFVLGFVVPSSSGLAVLSMPILAPLADTV
                     GIPRSVVVMAYQFGQYAMLFLAPTGLVMATLQMLDMKYSHWLKFVWPVVLFLLIFGGG
                     LLVLQVLIL"
     gene            complement(4656..>5656)
                     /locus_tag="VBE22_001746"
     CDS             complement(4656..>5656)
                     /locus_tag="VBE22_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO7203179.1"
                     /translation="QYVDIPISGKNQWLRAIRTVDRLDKQLTELLFSLGIVLPLMLII
                     ITVGGYLILKRTFRPIQEITETAQFITQNEDYTKRIITKNNENELTELAAVINTMLAS
                     IESSFVREKQFNNDVSHELRTPVTVILSESEYGKNYAENLSEAKESFEVIHRQSLSMK
                     KLVEQLLELTKAENPLSIQLEPLNFSIMMKQLVSDSSRLLDNTPIHLDSQIEDDLWII
                     GQQTLLKRLFDNLFSNAIKFTNNHISISLRQSDNQIVFSIKDNGLGISVDDQSKIWNR
                     FYQVDSARTKDSQSGIGLGLSLVKQIATIHRAKIWVDSKPDDGSQFTLTFPKLKKD"
BASE COUNT         1833 a          864 c         1106 g         1853 t
ORIGIN      
        1 ctagtataag ataaaacaat ttgaaagata tctttcaatg ccatagttga ttcatttttt
       61 acactaaaat aggttcctaa gcctttaata atattttggt aatctttctt aacttcgtga
      121 acgtcccgcg aaatatctag agagaggtgt tggtattctt taggtagatg atatttggat
      181 aactctttat ctaataaata agcatttttc atgatgtttt caatttcaat gatatttttt
      241 tgcataaaat aaacctcgct tttaacggct gaagttgacc acataaaata atagtatcga
      301 cgttcgtgat cgtctttttg taagagaagt gatagataac tataagtata agccacagcg
      361 caactaataa aagctcgcaa aagtgctatg gcaaataaag tagcgaatat agttatagta
      421 taattgttaa actttatgag aaaactaatt tctacaaggt tggcaaaata atcacaaata
      481 atgatagaaa agaaaaaagt acctttatta cggtaggaac gatgccaata aatagtataa
      541 aaggtgatgc cataacaaat ataaaacgcc atatcagtta aaacgaattc aattatttga
      601 tgaatctcag cctcgccagc gatacttaat atgattcctc tgaagatagg agaagcaaaa
      661 gtaattccta gacagagaag aattggatta atgtcgtcat taaaatataa aaagacaggc
      721 aaaatgaaca aagataaggt cataataaac ccatcagcaa gtattgagat attgatttga
      781 gcagctaagg cgatagctat tgcagagata ataatacgct gccttctttc tagatgtttt
      841 tctgataata tatctagcat ataaatgtcc ttaaagtttt tttgaagatt tttgatgaat
      901 tttgattatt tttgaaagcg atatctacaa taagagtata aaaaaaaagg aggcaataag
      961 caatgaaaaa tttacgtaac cgtagttttc ttactttatt ggattttagt acagcagaag
     1021 ttgaatttct tttaaaactt tcagaagatt taaaacgtgc taaatacgca ggaattgaac
     1081 aacaaaaact tgttggtaaa aatatcgctt tgatttttga aaaagattct acacggacac
     1141 gttgcgcttt tgaagttgct gctcacgatc aaggagctca tgttacttat ttaggtccaa
     1201 caggcagtca gatgggtaaa aaagagacct caaaagacac tgcgcgtgtg ctaggtggta
     1261 tgtatgatgg tattgagtat cgtggatttt ctcaagaaac agttgagact ttagccgaat
     1321 tttcaggagt tcctgtgtgg aatggtttaa ccgatgctga tcatccaacg caagtactag
     1381 ccgattttct gaccgctaag gaatgcttac ataagcccta taaagatatt cgctttacgt
     1441 atgtgggtga tggacgtaac aatgtcgcta atgccttgat gatcggtgct tctattgtag
     1501 gcatgactta tcatttggtt tgtcctaaag aattagagcc agatcctgaa ctactctcta
     1561 aatgtcaaga aattgcgaaa actactggtg cttctattga aatcactgct gatattgcag
     1621 aaggcgtcag agattcagac gtcctatata cagatgtttg ggtttcaatg ggagaaccgg
     1681 atgaagtttg gaaagaaaga atagctcttt tagaacccta tcgtattacc caagaaatgc
     1741 ttaatatgac agaaaatcca aacgttattt tcgagcattg cttgccctca tttcataata
     1801 tagatactaa agttggatac gacatttatg aaaaatatgg tttgaaggaa atggaagtat
     1861 cagatgaagt ttttgaaggc cctcactcag ttgttttcca agaagctgaa aatcgtatgc
     1921 atactattaa agcagttatg gtagcaactt taggtgatta ggaggaattt tcatggcaaa
     1981 aattgttgtt gcactaggag gaaatgcctt aggaaattct ccagaagaac aattaagatt
     2041 agtaaaacat actgcaaaat cattggtggc cttgataaaa aagggacacg aaattgttgt
     2101 aagtcatggt aatggaccac aagttggtgc cattaattta ggaatgaatt ttgcggctga
     2161 gagtggtcaa ggaactaatt ttccttttcc agaatgtgga gcaatgagcc aaggatacat
     2221 aggctatcat ctccaacaga gccttttaaa tgaattacgt caagagggaa taaataagga
     2281 agttgctaca attattaccc aaattgaggt ggatgaatca gaccaagcat ttagcgcacc
     2341 taccaagcct ataggcacct tttatgataa ggagacatca gaaaagattg ctatagagaa
     2401 agggtatact ttcgtagaag acgctggacg agggtatcgt cgtgttgttg cttcacctga
     2461 acctaaaaaa atcatcgaaa ttaatagcat taaaacactt attgaaaatg ataccctagt
     2521 tatcgcaggt ggaggtggag gcatccctgt cattaataag ggaggttatg aaggtattgc
     2581 agctgttatt gataaagaca aatctagtgc actgttagca ggagaactag ctgcggatca
     2641 attgattatt ctcactgcag ttgattatgt ttatactcaa tttggtaaag aaaatcaaaa
     2701 agctttaaca gaggttaatg agaatcaaat gattgattac gttaaccaag gtgagtttgc
     2761 aaaaggaagt atgttaccta aagtaatagc ctgtatgagt ttcttagatc acaatccgaa
     2821 aggaacagcc ttaatcacct cattaaatgg tttagaagat gcattagatg ggaaattagg
     2881 aacacgtata actaagtaat ctcagataag tgcaaagaaa tgattattta accttcatct
     2941 ttgcactttt tctgatgaag tcaatctatt tttattatag aggaggcgtg tcatgccaga
     3001 aaaaatgaca aacagtgaaa acaaaaggag taaaggtttt cgaatgccag gtgcctttac
     3061 aatattattc attttaacaa tcttttcggt tttggctaca tggtggatac ctgctgggtc
     3121 ctattcaaaa ttacaatttg atacagcatc atccaaacta gtagtaacag atccaaatgg
     3181 taagacagtt catgttccag caacgcaaac acagttggat aagatgaatg tcaaaattaa
     3241 aattaaagaa tttacttctg gtgctattag taagccagtt tcagttccaa atacttacaa
     3301 acgtttgaag caaaatccag caggcattgg ctccgtgacg actagtatgg taaatggaac
     3361 cattgaagct gttgatatta tggtatttat catggtactt ggaggcatga taggagtagt
     3421 tagaaagagt ggcgcttttg agtctggtct tctagcttta acaaagaaaa ccaaaggtag
     3481 agagttttta ttgatatttt tagtttcctt attgatggtt ttaggcggta ctctatgtgg
     3541 tattgaagaa gaagcagttg ctttttatcc tattttagtt ccaatctttt tagccatggg
     3601 ttatgattct atcatttgtg taggtgctat tttcttagca agttctgttg ggacatcatt
     3661 ttcaacaatt aatccgtttt cttctgttat tgcttcaaat gcagctggta ttagtttcac
     3721 agaaggctta tcatggcgta cagcagggtg tattgcaggt gctatttttg ttgtggtgta
     3781 tctacattgg tatgctaaaa aaataaaagc gaatccagag ttttcatatt cctatgaaga
     3841 tagagtagaa tttaatgcta aatggggcat gacaactaac catacaccct cactctttac
     3901 tattagacaa aaaattattt taagtttatt tgttatttca tttcctttga tggtttgggg
     3961 agtaatgtca cagggatggt ggtttccaac catggcttct tcctttttag ctattacaat
     4021 tattattatg tttttaacag caactggggc aaatggtatt ggagaaaggg atgttgttga
     4081 tgagtttgtt aatggtgcat caagccttgt tggggtttca ttaatcattg gtttagcgcg
     4141 tggtatcaat attattttga gccaaggtta tatatcagat acgatgcttt acacagcttc
     4201 aaaattagct tcacatgtta gtggctctgt ctttatcatt gttatgatgt ttatctactt
     4261 tgtgttagga tttgttgttc catcctcgtc gggtttagca gtactttcaa tgccaattct
     4321 agcacctctt gcagatactg ttgggattcc tcgttcagta gtagtaatgg cttatcaatt
     4381 tgggcagtat gcaatgttgt ttttagcacc tactggacta gttatggcaa ccttgcagat
     4441 gttagatatg aagtactccc attggctaaa atttgtgtgg ccggttgttt tattcctcct
     4501 aatatttggt ggcggtttgt tagttctaca agttttaata ctttaaagat ttcagctatt
     4561 agtctcaaaa atatctgaag agatataata aattgagttt aagttaaata aaaaaaacga
     4621 taggtgtaat ctatcgtttt ttttatttcc tttctctagt ctttcttcaa ttttggaaag
     4681 gtgagcgtaa actgactgcc atcatcaggt ttagaatcaa cccatatttt agcacggtgt
     4741 attgtagcga tttgttttac taaagatagc cctaaaccaa ttccagactg actatccttg
     4801 gttcgagcag agtccacttg atagaaacgg ttccaaattt tactttggtc atcaactgag
     4861 atacctagac cattatcttt aatagagaag acaatttgat tatcggactg tcttagacta
     4921 atagaaatat gattgtttgt aaatttaata gcatttgaaa atagattatc aaaaagccgt
     4981 ttgagtagtg tttgttgacc tataatccaa aggtcatctt cgatttgaga gtcaaggtgt
     5041 ataggggtat tatctaataa tctactcgaa tcggatacta gttgcttcat cataattgag
     5101 aaattaagag gttctaactg aattgataat ggattttctg ccttagtcaa ttctagaagt
     5161 tgctcaacta attttttcat tgatagtgac tgacggtgta taacctcaaa ggattctttt
     5221 gcttcactta aattttctgc gtagtttttt ccgtattcac tttcagaaag tataactgtt
     5281 acaggagtcc gtagttcatg agaaacatca ttgttaaatt gtttttcccg aacaaatgat
     5341 gattcaatag aagccaacat agtattgata acagctgcta gctcagttaa ttcattttcg
     5401 ttatttttag tgataattcg cttagtatag tcttcatttt gggtaataaa ttgagcggtt
     5461 tcagtaattt cttgaatcgg tctaaaagtg cgctttaaaa ttaaatagcc accaacagtt
     5521 attataatca acataagagg aagaacgata cccaaactaa ataacaattc agttaattgc
     5581 ttatccagtc tgtcaacagt acggatagca cgtaaccact gatttttccc agaaattgga
     5641 atatcaacat actgaa
//
LOCUS       DATRTN010000097         5627 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.097, whole genome shotgun sequence.
ACCESSION   DATRTN010000097 DATRTN010000000
VERSION     DATRTN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.097"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(21..815)
                     /locus_tag="VBE22_001747"
     CDS             complement(21..815)
                     /locus_tag="VBE22_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO7203180.1"
                     /translation="MDKLILGRYIPGNSLIHKLDPRSKLLAMLLFIIIVFWANNVVTN
                     VIVFIFTLVIVGLSQIKFSYFFNGIKPMVGIILFTTLFQMLFAQGGQVIFSFWIFSIT
                     SLGLQQAALIFMRFVLIIFFSTLLTLTTTPLSLADAVESLLKPLEVLRVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIHKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGANRSKYRLLKWTVRDTFSILLMLLLGLSLFLLKN"
     gene            complement(808..1650)
                     /locus_tag="VBE22_001748"
     CDS             complement(808..1650)
                     /locus_tag="VBE22_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO7203181.1"
                     /translation="MGIEFKNVSYTYQAGTPFEGRALFDVNLKIEDASYTAFIGHTGS
                     GKSTIMQLLNGLHIPTKGEVIVDDFSIKAGDKNKEIKFIRQKVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGISQIEAERLAEEKLRLVGISEDLFDKNPFELSGGQMRRVAIAGIL
                     AMEPKVLVLDEPTAGLDPKGRKELMTLFKNLHKKGMTIVLVTHLMDDVADYADYVYVL
                     EAGKVTLSGQPKQIFQEVELLESKQLGVPKITKFAQRLSHKGLNLPSLPITINEFVEA
                     IKHG"
     gene            complement(1626..2465)
                     /locus_tag="VBE22_001749"
     CDS             complement(1626..2465)
                     /locus_tag="VBE22_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO7203182.1"
                     /translation="MTNIITVNNLFFKYDSNQTHYQLENVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGQIIIDGQELTEDNVWELRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIPLKDMKERVDQALDLVGMSEFKMREPARLSGGQKQRVAIAGAVAMRPQ
                     VIILDEATSMLDPEGRLELIRTIRAIRQKYNLTVISITHDLDEVALSDRVIVMKNGKV
                     ESTSTPKALFGRGNRLISLGLDVPFTSRLMAELAANGLDIGTEYLTEKELEEQLWELN
                     LKM"
     gene            complement(2465..3007)
                     /gene="pgsA"
                     /locus_tag="VBE22_001750"
     CDS             complement(2465..3007)
                     /gene="pgsA"
                     /locus_tag="VBE22_001750"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEO7203183.1"
                     /translation="MMKKENIPNLLTVVRILMIPLFIVLTSVTTSTTWHIVAAIVFAI
                     ASLTDYLDGYLARKWQVVTNFGKFADPLADKMLVMSAFIMLVGLDLAPAWVSAIIICR
                     ELAVTGLRLLLVETGGTVLAAAMPGKIKTATQMFAVIFLLVHWMTLGNIMLYIALFFT
                     LYSGYDYFKGAGFLFKDTFK"
     gene            complement(3130..4413)
                     /locus_tag="VBE22_001751"
     CDS             complement(3130..4413)
                     /locus_tag="VBE22_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO7203184.1"
                     /translation="MTVNKITYQNLQEEVYKLTLESGLNVYLIPKPSFKETVGVLTAN
                     FGSLHTKYTRNGCVEHYPAGIAHFLEHKLFELDKGQDAATQFTKYGAESNAFTTFDKT
                     SFYFSTISHITNCLDILLDFVLTTNFTEESITKEKDIIKQEIEMYQDDPEYRLYQGVL
                     SNLYPNSPLAFDIAGDYQSISQITLTDLQENHKDFYQLSNMNLVLVGQFSPQEIITYL
                     QKNSHFTSYSQNIDRDSISLEPVIKNNSCHMTVTKPKLAIGYRKSNHMIHGSYLKEKI
                     GLQLFFAMLLGWTSTINQDWYESGQIDDSFDIEIEVHPDFECVIISLDTTEPIAFSTQ
                     LRLLLKNALQSSDLTESHLKNVKRELYGDFLRSLDSIENLAMQFVTYLYDGKTMYLDL
                     PSIVEELDLEDVITIGKDFLDNADTSDFVIFPKSS"
     gene            complement(4415..>5627)
                     /locus_tag="VBE22_001752"
     CDS             complement(4415..>5627)
                     /locus_tag="VBE22_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO7203185.1"
                     /translation="IKNQQFKTNHLTFRFSGDFNNKTVARRSLVAQMLVTANAKYPKV
                     QEFREKLASLYGASLSTKISTKGLVHIVDIDIVFVKNTFTLEQENIVEQIITFLEDML
                     FSPLISLEQYQTSIFDTEKKNLIQYLEADIEDNFYSSDLALKSLFYNNKTLRLPKYGT
                     ASLVESENSFTAYQEFQKMLKEDQLDIFVVGDFDDYRMIQAFNRMAFEPRHKVLAFDY
                     TQTYENITRSQVEDKDVNQSIMQLAYHLPITYKDEDYFALIVFNGLFGAFAHSLLFTE
                     IREKQGLAYTIGSQFDSFTGLFTIYAGIDKENRERFLKLINKQFNNIKMGRFSSTLLK
                     QTKDILKMNYVLASDNPKVIVDHIYHEHYLDQFHTSALFIDKVDDVTKSDIVSVATKL
                     KLQAFYFLEGN"
BASE COUNT         1889 a         1054 c          844 g         1840 t
ORIGIN      
        1 cataaactga tcttaacctt ttaatttttt aatagaaaca aactcaaacc caataataac
       61 ataagtaata tactaaaagt atcccgaaca gtccacttca ataaacggta cttactacga
      121 ttggctccgc cttgataacc tcttgcttcc atagcaattg ccaacgcatc tgctctttta
      181 aaactagaag caaataatgg aatcaaaata gggataatag acttaacctt atgaattagg
      241 tttccctctc caaaatcaac tccacgagct ctttgagcat tcatgatacg tgttgtatca
      301 tccattaaag taggaacgaa acgtaagcta agggataaca tcaaaccaat ctcgtgtgct
      361 ggtactctca gtacttccaa tggttttaac aaagattcaa cagcatctgc taaacttaac
      421 ggagtagtag ttaaggtaag taatgtcgaa aagaagataa ttaaaacaaa tctcataaaa
      481 ataagtgctg cttgttgtaa cccaaggcta gtgatgctaa aaatccaaaa cgagaagata
      541 acctgcccgc cttgtgcaaa cagcatctgg aagagggttg taaataaaat aataccaacc
      601 atcggtttaa taccattgaa aaaataggaa aattttatct gagataaacc cacaataacc
      661 aacgtaaaaa tgaatacgat cacatttgtc actacattat ttgcccaaaa aacaataatg
      721 ataaaaagca acatggctag taacttactt cgaggatcta atttatgaat gagagaatta
      781 cctggtatgt aacgtcccaa aatcaattta tccatgctta atagcctcca caaattcgtt
      841 aatagtaatt ggtaaactag gtaaatttaa tcccttatga gatagtcttt gagcaaactt
      901 ggtgattttg ggaactccta attgtttact ttctaaaagt tctacttctt gaaaaatctg
      961 ttttggttgt cctgataagg ttactttccc tgcttctaaa acatacacat agtcagcata
     1021 atccgctaca tcgtccatta agtgagtcac taagacgata gtcattcctt ttttatgaag
     1081 atttttaaaa agagtcatta attcttttct tcccttagga tcaagtccag ctgttggctc
     1141 atccagtact agtactttgg gttccatcgc taaaatacca gctatagcaa cccgcctcat
     1201 ctgccctcca gaaagttcaa atggattttt atcgaataaa tcctcactga taccaactaa
     1261 ccttaatttt tcttcagcca gcctttcagc ttcaatctga gaaataccaa aattttgtgg
     1321 tccaaaagca acatccttta aaactgtctc ttcaaaaagc tgactttctg gaaattgaaa
     1381 aactaaacca actttttgcc ttataaattt gatttctttg ttcttgtccc ctgctttaat
     1441 agaaaaatca tcgacaatta cctcaccttt tgtaggaata tgtaaaccat tcaaaagttg
     1501 cataatagtt gattttccag aacctgtgtg cccaatgaac gcggtatagg aagcatcttc
     1561 aattttcaga ttgacgtcaa aaagggcacg cccttcaaaa ggagtgccgg cttgataggt
     1621 ataacttaca tttttaaatt caattcccat aattgttctt ctaattcctt ctctgtaaga
     1681 tactctgtcc ctatatcaag accattagca gccagttctg ccattaacct acttgtaaag
     1741 ggaacatcta aacctaaact aatcaaacga tttccacgac caaataacgc ttttggagtg
     1801 gatgtcgact caactttacc atttttcata acgatgacac gatcactcaa tgcaacttca
     1861 tctaagtcgt gtgtaattga aataacagta agattatatt tttgacgaat agctcgtatc
     1921 gttcttatca gttccaatcg tccctcagga tcaagcatac tagtcgcctc atccaagatg
     1981 ataacttgtg gacgcatagc tacagctcct gcaatggcta cacgctgctt ttgtcctcca
     2041 gataaacgtg caggctccct cattttaaat tcagacattc ctaccaaatc aagtgcttgg
     2101 tccactcttt ctttcatgtc tttcaaagga atacctttat tttccaaacc aaaggcgacg
     2161 tcatcttcaa cagttgcccc tacaaattgg ttatcaggat tttgaaatac catgccaatc
     2221 ttgtgacgta actcccagac attatcttcg gttaactctt gtccatcaat gataatctgg
     2281 ccagactccg cctctaaaag tccatctatt aaacgaacag tagtggattt cccagaacca
     2341 ttatgaccaa taatagatag ccattctcct tgtttcacat gaaacgatac attctccaat
     2401 tgataatgtg tttgattact atcatattta aaaaagagat tattaacggt aataatattt
     2461 gtcattattt aaaagtatcc ttaaataaga agcctgcacc tttaaaatag tcgtagcctg
     2521 aatacaaggt aaagaataga gcaatgtata acatgatgtt accaagtgtc atccaatgta
     2581 ctaacaaaaa aataactgca aacatttgag tagccgtctt tattttccct ggcatagctg
     2641 cagcaagtac tgttccacct gtttcaacta aaagtaaacg caaacctgta actgctaact
     2701 ctctacaaat gataatagct gaaacccaag ctggtgctaa gtctagtcca actaacatga
     2761 taaaagcact cataactagc attttatcag ctaatgggtc tgcaaatttc ccaaaatttg
     2821 ttactacttg ccatttacgt gctagataac catctaggta atcggtcaaa cttgcaatag
     2881 caaatacaat agctgcaaca atatgccaag ttgtacttgt tgttactgaa gttaatacta
     2941 taaataacgg aatcattaaa attctgacta cagttaatag attcggaata ttttctttct
     3001 tcatcataaa ttctcctttt ggaaacatag tattcctata ttatgttatt tcaaaactag
     3061 taaaagactt ttgacatacc aactttatag atgtctaagc ttccttatta tatcaaaatt
     3121 tcagattatt tacgaagatt ttggaaaaat aacgaagtca gatgtgtcag cattatctaa
     3181 gaaatcctta ccaatcgtaa tgacatcttc caaatctaat tcttcaacga tagatggcaa
     3241 atccaaatac atcgtttttc catcatataa ataggtgacg aattgcattg cgagattttc
     3301 aatagaatct aagcttctta aaaaatcgcc atagagctct ctcttaacgt tttttaagtg
     3361 gctttcggta agatcagatg attgcaaagc attttttagc aataacctca actgagttga
     3421 aaaagcaatt ggctctgtcg tatctaaaga aataataacg cactcaaaat ctggatgaac
     3481 ttcaatttct atatcaaaag aatcatcaat ttgaccagat tcgtaccagt cttggttaat
     3541 tgtagaagtc catcctaaaa gcattgcaaa aaatagctgt aatccgattt tctcttttag
     3601 gtaagaacca tgtatcatat gatttgattt tcgataacca atagctaatt taggttttgt
     3661 cactgtcata tggcaagagt tgttttttat aacaggctca aggctaatag aatctcggtc
     3721 aatattctgt gagtaacttg tgaaatgaga atttttttga aggtaagtta taatttcttg
     3781 tggactaaat tgtccaacaa gaactaaatt catattagac aactgataaa agtctttgtg
     3841 attttcttgt aaatcggtta aggttatttg agagattgat tggtaatctc cagcgatatc
     3901 aaatgctaat ggagagttgg ggtaaaggtt cgataacact ccttgataaa gcctatattc
     3961 tgggtcatct tgatacattt caatttcttg tttgataata tctttttctt tagtaattga
     4021 ctcttctgta aagtttgttg tcaatacaaa gtcaagtagt atatctaagc agtttgtaat
     4081 gtgacttata gttgaaaaat aaaaactagt tttatcaaaa gttgtaaagg cgttactctc
     4141 ggcgccatat ttagtaaatt gagttgcagc gtcttgtcct ttatccagtt caaataattt
     4201 atgttctaag aagtgagcaa ttcctgccgg ataatgttct acacaaccat tcctcgtata
     4261 ttttgtatgg agtgatccaa aatttgctgt caatactcca acagtttcct taaatgatgg
     4321 ctttggaata aggtatacat ttagtccgct ctctaatgtt aacttatata cttcctcctg
     4381 aaggttctga taagttattt tattaactgt catcctagtt tccttccaaa aaataaaaag
     4441 cttgtaattt taattttgtt gctacgctga caatatctga ttttgttaca tcgtctactt
     4501 tgtcaatgaa tagtgctgaa gtatgaaact gatctaaata atgttcatga taaatgtggt
     4561 caactatgac cttaggatta tcagaagcta aaacatagtt cattttcaat atatcttttg
     4621 tttgtttcaa taatgtagaa gaaaacctgc ccatcttaat attattgaat tgcttattaa
     4681 taagcttcaa aaaacgctca cgattctctt tatcaatacc tgcatatatc gtaaagagac
     4741 ctgtaaagct gtcaaattga ctacctatag tataggctaa accttgcttt tctcgaattt
     4801 ctgtaaacaa caaggagtgg gcaaaagcac caaataaacc gttaaatact attaaagcaa
     4861 aataatcttc atctttataa gttattggaa gatgataagc caattgcata atagattgat
     4921 taacgtcctt atcttctact tgacttctcg ttatattttc ataagtctgc gtataatcaa
     4981 aagctaatac tttgtgacgt ggctcaaatg ccatacgatt aaaagcctga atcattcgat
     5041 agtcatcaaa atcccctacg acaaaaatat ctaattgatc ttccttaagc atcttctgaa
     5101 attcttgata agctgtaaat gaattttctg attccactaa agatgctgta ccatacttag
     5161 gtaaacgaag tgtcttatta ttgtagaata aagattttag tgctaagtca ctgctataaa
     5221 aattatcttc aatatcagcc tctaaatatt gaataaggtt tttcttctca gtatcaaaaa
     5281 tagatgtttg atactgttct aaagaaatta aaggagaaaa cagcatatcc tccaagaatg
     5341 taataatttg ttcaacgata ttttcttgtt ctaaagtaaa ggtattttta acaaatacaa
     5401 tatcaatatc aacaatgtga acaagaccct tagtagaaat cttagttgat aaactagcgc
     5461 catataagga agctaacttt tctcgaaatt cttgtacctt aggatactta gcatttgcag
     5521 taactaacat ttgagcaacg agcgatctac gtgctactgt tttattatta aaatcacctg
     5581 aaaatcgaaa agtaagatga ttagtcttaa attgttggtt tttaatt
//
LOCUS       DATRTN010000098         5623 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.098, whole genome shotgun sequence.
ACCESSION   DATRTN010000098 DATRTN010000000
VERSION     DATRTN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.098"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            271..1413
                     /gene="tgt"
                     /locus_tag="VBE22_001753"
     CDS             271..1413
                     /gene="tgt"
                     /locus_tag="VBE22_001753"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEO7203186.1"
                     /translation="MTDHPIKYRLIKQEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIAKAGGLHKFMNWDQAILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGAKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLNAHRRPHDQGLFGIVQGAGFEDLRRQSARDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTVPMLPNDKPRYLMGVGAPDSLIDAVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKFAEDFTPLDPNCDCYTCKNYTRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKDVRQAIMDDNLLEFRQDFMERYGYGTNNRNF"
     gene            1519..1830
                     /locus_tag="VBE22_001754"
     CDS             1519..1830
                     /locus_tag="VBE22_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203187.1"
                     /translation="MMQEYFGIGLDSSSRCYHYHTKLDIVALKCAVCQKYYACYKCHD
                     ALEEHCFAATKSDEMFPVLCGSCRQMLTLKEYKTGFCPYCRMLFNPNCQRHDSIYFSK
                     E"
     gene            1837..2376
                     /locus_tag="VBE22_001755"
     CDS             1837..2376
                     /locus_tag="VBE22_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO7203188.1"
                     /translation="MFHTKSLIQMAMMATLLVVLGFIPALPLGFIPVPIVLQNLGVMM
                     AGVILGGRKGSLSILLFFLVGLVIPVFSGLRSTIPVLTGPTAGYVIAWLFVPLLISYG
                     VKLVNKKNFIAIFLIVWLAGVLFVDVAGAVWLASYTNIPLDKSLLQNLVFLPGDTIKA
                     IIAAVVAVKYKDSFFLTEK"
     gene            2515..3291
                     /locus_tag="VBE22_001756"
     CDS             2515..3291
                     /locus_tag="VBE22_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO7203189.1"
                     /translation="MKLTTLGCWGGYPYKDGGTTSFLLTGENNFHLLIDAGSRSINEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLYHYRLLYPKDKWEPKVLPVYGHNEDDYEFS
                     KLTLPNVAQGIAYHTNGVEQVGPFNITFIKTVHPVVCYAMRITERETGQVLVFTGDSG
                     YFADLVPFTKGADLFLADVYLYEGNENHIAHLTSKEAGEIAYNAKVKKLVLTHVPQFP
                     GEGINPENHLEELRLQSEKYAGGISVDLAQPHKSWDLGKL"
     gene            3291..3797
                     /locus_tag="VBE22_001757"
     CDS             3291..3797
                     /locus_tag="VBE22_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEO7203190.1"
                     /translation="MQKFTQEEKEWFMTEALKEAKSSLEKEEIPIGCVIVKDGHIIGR
                     GHNAREEFNKAILHAEIMAINNANEKVGNWRLLDTTLFVTVEPCVMCSGAIGLARIPH
                     VIYGAKNTKFGAAGSLYDILSDSRLNHRVEVETGILEDQCAKIMQDFFRKGRQRKKEA
                     KLSEKTKI"
     gene            3832..3917
                     /gene="ffs"
                     /locus_tag="VBE22_001758"
     ncRNA           3832..3917
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VBE22_001758"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4069..5418
                     /locus_tag="VBE22_001759"
     CDS             4069..5418
                     /locus_tag="VBE22_001759"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEO7203191.1"
                     /translation="MTHITFDYSKVLGQFVGEHELDYLQPQVSAADAFLRQGTGPGSD
                     FLGWMDLPENYDKEEFSRIQKAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNNHFAN
                     LQTAEERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVVRVEK
                     PRKNVTIPELTEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPTQDAY
                     TLGYTIYFFELAIGLSGYLNSVNPFDQPGVEAYKRNMFALLGKPGFEELSAELNARL"
BASE COUNT         1808 a          892 c         1122 g         1801 t
ORIGIN      
        1 aatcgggagt aaaaaaagtt gattcttacc ttgaagattg cttaatatag tttttgcctc
       61 atttttaatt tctgaattcg tcatatctcc tccatacata aaagtacaat tcatttcatt
      121 ataacacatt ccttaagatg agcatgacaa gagtacattt ttttgataaa atcttaatgg
      181 ctaaaaaaga aattaataca aagtattaca tttagatatt gattagcact aaagcccatg
      241 actgtttttc atggaagcaa ggagaaaacg atgactgatc atccaataaa atatcgatta
      301 attaaacagg aaaaacacac aggagccaga cttggtgaaa taatcacacc acatggtaca
      361 tttccgacac caatgtttat gcctgttggg acacaagcaa ctgttaagac acaatctcct
      421 gaagaactaa aagaaatggg ttcaggaatt attctatcaa atacctacca tttatggtta
      481 cgtccaggtg atgaattgat tgctaaagct ggaggtttac ataaattcat gaactgggat
      541 caggctattc taacagacag tggaggcttt caggtttatt ctttggctga tagtcgaaac
      601 atcactgaag aaggagtaac ctttaaaaac catttaaatg gtgcgaaaat gtttttatca
      661 ccagaaaaag ctatttcaat tcaaaataac cttggttctg atattatgat gagttttgat
      721 gagtgtccac aattttacca gccttatgat tatgtaaaaa aatcaattga gcgaacaagc
      781 cgttgggcag aacgtggatt aaatgcccac cgccgtcctc atgatcaagg actatttggt
      841 attgttcaag gggcagggtt tgaggattta cgtcgtcaat cagcacgaga tttagtatcg
      901 atggattttc caggttattc aattggaggg cttgcggtcg gagaaacaca tgatgagatg
      961 aatgcagttc ttgattttac tgttccaatg cttccgaatg ataagcctcg ttatttgatg
     1021 ggagttggtg caccagatag tttaatagac gccgttatcc gaggagtaga tatgtttgac
     1081 tgcgtcttac ctactcgtat tgctcgaaat gggacatgta tgacaagtca aggtcgttta
     1141 gttgttaaga atgctaaatt tgcagaagat tttacgcctc ttgatccaaa ttgtgattgc
     1201 tatacttgta aaaattatac acgcgcttat atccgtcact tattgaaagc agatgaaact
     1261 tttggtattc gtttgactag ttaccataat ctttatttcc ttgtcaatct tatgaaagat
     1321 gtcagacaag ctattatgga tgataatctt ctagagttcc gtcaagattt tatggagcgc
     1381 tatggttatg gcacgaataa ccgtaatttt taaaaaagtt cctggtaaaa cagggctttt
     1441 tttgatctgt ttgttaacct cgttttaaaa aaagttgaca atggaaaaac ttttgtataa
     1501 tatgttatag gaggaatcat gatgcaagag tactttggta ttggtcttga tagtagcagc
     1561 cgctgttacc actatcatac taaattggat attgtcgctt taaaatgtgc tgtttgtcaa
     1621 aaatattatg cttgttataa atgtcatgat gcactcgagg agcattgttt tgcagcaaca
     1681 aaatctgatg aaatgtttcc tgtcttatgc ggttcttgtc gtcagatgtt gacattaaaa
     1741 gagtataaaa ctggtttttg tccttattgt cgtatgcttt ttaatccaaa ttgtcaacgc
     1801 catgattcta tttatttttc gaaggagtaa tttttaatgt ttcacacaaa aagcctaatc
     1861 cagatggcta tgatggctac tttattggtg gtacttggtt tcattcctgc tctaccactt
     1921 ggtttcattc cggtccctat cgtcttacaa aacctagggg ttatgatggc tggtgttatt
     1981 ttgggcggaa gaaaagggag tttatctatt ttgctcttct ttcttgtcgg tttagttatt
     2041 cctgtattct caggtttacg ttcgactata cctgtattga caggaccaac agctggttat
     2101 gttattgctt ggttgtttgt tcctttgctt atctcatatg gtgtaaaatt agtaaataaa
     2161 aagaatttta tagctatttt ccttattgta tggctagcag gggtcctttt tgtagatgta
     2221 gcaggagcag tttggctggc atcttatacg aacatcccat tagataaatc acttcttcaa
     2281 aacttagtat ttctcccagg agatacaata aaagccatta tagctgctgt tgttgctgtt
     2341 aaatataaag atagtttttt cttgactgaa aaataatcaa gattgctata ttttagatgg
     2401 actagtagat ctaaaaattt agatttacta gtcttttttg tgcataaaat ctaaaacagt
     2461 tattcttaac aaaatttggt ataatagcat taaatcagta aggaggggat tatcatgaaa
     2521 ttaacaacgt taggttgttg gggtggttat ccttataaag atggaggaac cacaagtttt
     2581 ctattaactg gagaaaataa ttttcactta ttaatagatg cgggaagtcg ttcaatcaat
     2641 gagttagaaa aagaaataag cccgttggat ttggatgcag ttatcatcag tcattatcat
     2701 ccagatcatg tagcagattt gggtgtgttg tatcattatc gtttgctata ccctaaagat
     2761 aaatgggaac ccaaagtatt gccagtttat ggacataatg aggatgatta tgagttttct
     2821 aaattgacat tacctaacgt tgctcaagga attgcttatc ataccaatgg agttgagcaa
     2881 gttggtccct tcaatattac gtttatcaaa acagtccacc cagtcgtctg ctatgccatg
     2941 aggattacag aaagagaaac tggtcaagta ttagtcttta caggagattc tgggtatttt
     3001 gctgaccttg taccttttac aaaaggcgca gatttatttt tagcagacgt ttatctttac
     3061 gagggaaatg aaaatcatat tgcacatttg actagtaaag aagcaggtga gattgcttat
     3121 aacgctaaag ttaagaaatt agttttaaca catgttccgc agtttccggg agaaggaata
     3181 aaccccgaaa atcatttgga agaacttcga ctacagtctg aaaagtatgc aggtggcatc
     3241 tctgttgatt tagctcagcc acataaatct tgggacttag gaaaactata atgcagaaat
     3301 ttacccaaga agaaaaagaa tggtttatga cagaagctct aaaggaggca aaatcctcct
     3361 tagagaaaga agaaattcct ataggttgtg tcattgtaaa agatggtcat attattggaa
     3421 gaggacataa tgctcgtgaa gagtttaata aagctatttt acatgctgaa atcatggcta
     3481 ttaacaatgc aaatgagaag gtagggaatt ggcgcttatt agatactact ttatttgtta
     3541 ctgtagaacc ttgtgtaatg tgtagtggag ctattggctt agctagaatt ccccacgtta
     3601 tatatggcgc taagaatacg aaatttggag cagcgggaag tctctatgat attttaagtg
     3661 atagccgact taatcataga gttgaagttg aaaccggcat tttagaagat caatgcgcta
     3721 aaataatgca ggatttcttt cgaaaaggac gacaacgcaa gaaagaagct aaactaagtg
     3781 aaaaaactaa gatttgatag cttgcaataa aatattagtt ttgttataat atctttggag
     3841 caacatttac tcgtgaagtg ggtcagggga ggaatccagc agccctaagc gatgctaaat
     3901 gtgtgctctt tttattttca aaagtcaaag tgtttttgga aataattatg aaagtttcat
     3961 aaaaattggt agcgtttcaa aaaatatgaa aatacagtta aaaaaccttg aaaaaaaact
     4021 cacaaaagag taagatgata gtgaaacata attgttagga gaaataaaat gacacatatt
     4081 acatttgact attcaaaagt cttgggtcaa tttgtaggcg aacacgagtt agactaccta
     4141 caaccacaag taagtgcagc agatgctttc cttcgtcaag ggactggtcc tggatcagat
     4201 tttctcggat ggatggacct cccagaaaac tatgacaaag aagaattttc tcgcattcaa
     4261 aaagccgctg aaaagattaa atcagatagc gaagtactcg tggttattgg tattggtggt
     4321 tcgtaccttg gtgcaaaagc agcaattgac tttttgaata atcattttgc taatttgcaa
     4381 accgcagaag aacgtaaagc gcctcagatt ctttatgctg gaaattctat ttcatctact
     4441 taccttgccg atttagttga atacgtccaa gataaagaat tctcagtaaa tgtcatttca
     4501 aaatcaggta caacaactga accagcgatt gctttccgtg tatttaaaga acttctagtt
     4561 aaaaagtacg gtcaagaaga agctaataaa cgtatctacg ctacaactga taaagttaaa
     4621 ggtgccgtaa aagttgaagc agatgctaac aattgggaaa catttgtagt tcctgataat
     4681 gttggtggtc gtttctcagt actgacagct gtaggtcttc ttccaattgc tgcttcagga
     4741 gcagatatta ctgctcttat ggaaggcgca aatgctgctc gtaaagattt atcttcagat
     4801 aaaatctctg aaaacattgc ttatcagtac gctgcagttc gtaatgttct ttatcgcaaa
     4861 ggttatatca cagagatttt agctaactac gaaccatcat tacaatactt tggtgaatgg
     4921 tggaaacaat tagcaggtga atcagaaggt aaagatcaaa aaggtatcta tcctacatca
     4981 gcaaacttct caactgatct tcactcactt ggtcaattta ttcaagaagg ttaccgtaac
     5041 ctctttgaga cagttgttcg cgttgagaaa cctcgtaaaa atgtgactat tcctgaatta
     5101 accgaagatt tagatggtct tggttatctt caaggaaaag atgtagattt tgttaataaa
     5161 aaagcaacag atggtgtgct tcttgctcat acagatggtg gggttccaaa tatgtttgta
     5221 acgcttccta cacaagacgc ttacactctt ggttacacta tttacttctt tgagttagca
     5281 attggccttt caggttatct taactcagta aatccatttg atcaaccggg ggtagaagca
     5341 tataaacgta atatgtttgc tcttctaggt aaacctggat tcgaagagct tagcgctgaa
     5401 ttgaatgcac gtctttaaaa atatctaact aaaaagttcc tcaaagagag gaacttttta
     5461 gttttgttgt aggataacta aaaacccact agcactggta gcgatagtgg gttggaaaaa
     5521 gggttgagaa aagttattaa cttttctcag gagatttatg aaaaaggaaa ataatttagg
     5581 ataatatttt cctaacggct aggataaacc gcttatttta gga
//
LOCUS       DATRTN010000099         5570 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.099, whole genome shotgun sequence.
ACCESSION   DATRTN010000099 DATRTN010000000
VERSION     DATRTN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.099"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            107..1480
                     /locus_tag="VBE22_001760"
     CDS             107..1480
                     /locus_tag="VBE22_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7203192.1"
                     /translation="MLKKLFAPPQKRERLSAEEIKHKYTRYRVQVLISIFVGYMGYYF
                     VRNTTSILSGILNMSATEIGIITCASYIAYGLSKFISGLISDESNSKIFLPVGLFLTG
                     LVNVLIGVIPSVITSIWLFTIMYLINGWLQGMGYPPGARTLVYWYDNKERIKYATIWN
                     LSHNFGGAIAPILTGVGLALAGNDSLNQARAAYWFPGVVACLLAVLVYFLQEDTPESI
                     GLPPIEEYHKEQYTNVVDSSDILEEPEVLGMGEIIKKYILPNTKLMWASLYSIFVYIL
                     RYGIVSWTPKFLATSVQDGGKGITATAGMGGFSLFEIGGIIGMLTAGYLSAKVFKNSK
                     PLTNVAFLVVAILLLAAYWFIPAGPQYMALDFIILLGLGASIYGPVMMVGLYAMELVP
                     KAAAGAASGLTGTFSYVGGATIATLAIGIIIDHFGWGVAFIIFGISGFAAIVCTLLSR
                     DKSLEYW"
     gene            complement(1638..1943)
                     /locus_tag="VBE22_001761"
     CDS             complement(1638..1943)
                     /locus_tag="VBE22_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203193.1"
                     /translation="MTYELCLEYGTYPLRPVDAWADEINTAPAFITEDKKLLELLEEV
                     NTLFHELFLTIECSFHYIGHDFPEKRAKITQIYHVIIEHLSIHYPEYDIKIESLLMG"
     gene            2106..3092
                     /locus_tag="VBE22_001762"
     CDS             2106..3092
                     /locus_tag="VBE22_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEO7203194.1"
                     /translation="MSEKIRVLLYYKYVSIENAEEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENEAKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPQVANV"
     gene            complement(3129..4256)
                     /locus_tag="VBE22_001763"
     CDS             complement(3129..4256)
                     /locus_tag="VBE22_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEO7203195.1"
                     /translation="MKHYQTIIIGAGAAGIGFGSAMQRLGLTNFLIIEKGHIGESFLR
                     WPRTTQFITPSFTTNGFGFPDLNAVIPDTSPAFSFEKEHLSGVEYARYLQLVAAHYNL
                     PIQNETSVLSIDKQDSLFVIKTSKGDFSADYLIMATGEFQNPNTIDIKGADLGMHYGQ
                     VDNFHIKSDNPFIIIGGNESACDALTHLVYLGNQVELYTDTFGRKESNPDPSISLSPL
                     TKERLKHIQDHKKEYYSISEGKKAIEIKQIGKQYQVTFDDGSTAESFHKPILSTGFLN
                     TCHLIDGIALFEYDKNQLPIVTEDDESTIVNNCFLIGPSLRQRDTIFCYIYKFRQRFV
                     PVINHIAQREGIILDEDTLEFFKENQMYLDNLACCDVNCDC"
     gene            4360..5379
                     /locus_tag="VBE22_001764"
     CDS             4360..5379
                     /locus_tag="VBE22_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEO7203196.1"
                     /translation="MFLLLCILSMWLMIFGVAFYYFGSLHQSLTSRIIYQFVLTVLLT
                     TTAWFMGAYFLAFEGHFKTVFQFQEADGKQIVNCLFQLCFALYAVVMLIGSIIDRVQT
                     KRLLLAVVSWLFLVYTPLAYLIWNSEGVFAKMGVLDFSGGMVVHLSAGLSSYILAHVI
                     GKSEHQHNKVKNDSLFLGMILITFGWFGFNMGPVGEWNSQAIMILLNTIFAIIGGGLA
                     WTLAAKWNGEEEKTGSLLNGIIVGLVTSTAGVGYLLTWQLLAVTFFASLFTYFVTDYV
                     AKAFAIDDVVSSFGMNGIGGLLGSLGVGLFKLSHMPVQLLALATTILLSIIMTYIISK
                     AIFRK"
     gene            5458..>5570
                     /locus_tag="VBE22_001765"
     CDS             5458..>5570
                     /locus_tag="VBE22_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEO7203197.1"
                     /translation="MNYEASKQLTDVRFKRLVSVQRTTFEEMLAVLKTAYQR"
BASE COUNT         1676 a          813 c         1131 g         1950 t
ORIGIN      
        1 taaaaaaata agataaaatg ataaaaaata aattattaat taattgacag tgtgaattca
       61 tagttgtata attaaacaca tctaaaaatc ggaaaggtaa tttgctatgt tgaaaaaact
      121 atttgcaccc cctcagaaaa gagaacgttt atcagctgag gaaattaagc ataaatatac
      181 tcgttatcgt gtacaagtcc ttatttctat ttttgtaggt tatatgggat actattttgt
      241 tcgtaatacg acttcaattt tatctggtat ccttaatatg tctgctactg agattggtat
      301 tattacctgt gctagctaca ttgcttatgg tctctctaaa tttatttctg gtttgatttc
      361 agatgaatcg aattctaaaa ttttccttcc agttggtttg tttcttacag gactagttaa
      421 tgtgttaatc ggtgttattc catctgtaat tactagtatc tggctcttta ctatcatgta
      481 tcttattaat ggttggttac aagggatggg ataccctcct ggagcgcgta cattagtcta
      541 ttggtatgat aataaagaaa gaattaaata tgctaccatt tggaatttat ctcacaattt
      601 tggtggagca atagcgccga ttttaactgg tgtaggtcta gctcttgctg gtaatgattc
      661 tcttaatcaa gcgcgtgctg cttattggtt tccaggggtg gtggcttgtc ttttggctgt
      721 tcttgtttac tttttacaag aagatactcc agaatctatc ggacttccgc ctatagaaga
      781 gtatcataaa gagcaatata ctaatgttgt ggattcaagt gacattttag aagagcctga
      841 agttttgggt atgggcgaaa ttataaaaaa atatattcta ccaaatacta aactgatgtg
      901 ggcatccttg tattcaatat ttgtatatat tttacgttac ggtattgtct catggactcc
      961 taagttttta gcgacgagtg tgcaagatgg tggaaaaggt attactgcaa cagctggtat
     1021 gggtggtttt tcactttttg aaataggtgg tattattggg atgctaacag caggatatct
     1081 atctgccaaa gtgtttaaaa actctaaacc tttaaccaat gtagccttct tagttgtagc
     1141 aatcttatta ctagctgcct attggtttat tccagcagga cctcaatata tggcattaga
     1201 cttcattatt ttactaggtt tgggggcatc aatctacggt cctgttatga tggttggcct
     1261 gtatgctatg gagttagttc caaaagcagc agctggagca gcttcaggat tgactggcac
     1321 atttagttat gtcggaggag caacgatcgc tactctagct attggtatta ttattgacca
     1381 ctttggttgg ggagtagcat ttataatatt tggtatttct ggatttgcgg ctattgtctg
     1441 taccttgctt tcaagagata agagtctaga gtattggtga tattatttgg taaaagtatc
     1501 atttaatatt tagtgagata agttatgaaa aaaggcctct tgaaagagac cttttttgtt
     1561 ttagataatc tatttgttga agtaattagt gatatcgatt gtttttaata gatagtttct
     1621 acctagttaa aataaaacta ccccattaat aatgattcaa ttttaatgtc atattcaggg
     1681 tagtgtatgc ttaaatgttc gataatcaca tgataaattt gtgtgatttt tgctcttttt
     1741 tctgggaagt catgaccaat atagtggaag gaacattcta tcgttaaaaa gagctcatgg
     1801 aataaggtat tgacttcttc taaaagttct aaaagttttt tatcttcagt aataaacgca
     1861 ggtgcagtat tgatttcatc agcccaagca tctacaggtc taagtggata agtaccgtat
     1921 tctagacata attcataagt cataaaagtc ctcctagtct tttactattt taacatatgt
     1981 gtgttcttaa gaagaataat ttgtctttaa agcaaagaat atgtcctaag cgtatttttt
     2041 gctatgttgt aaggagtcgt tatataatga agtatactaa cttattttgc agatgaggag
     2101 aaaaaatgtc agaaaaaatc agagttttac tctattataa atatgtttca attgaaaatg
     2161 cggaagagta cgctgcgaaa catctagaat tttgtaaatc tattgggctt aaaggtcgta
     2221 ttttgattgc ggatgagggg attaatggaa ctgtttctgg agattatgaa acaactcaga
     2281 agtatatgga ttgggtccac agcgatgagc gttttgctga tctttggttc aagattgacg
     2341 aggaaaatca acaagctttc cgtaaaatgt ttgttcgtta taagaaagag attgtgcacc
     2401 ttgggttaga agataataac tttgatagcg atattaatcc tttggaaaca acaggtgagt
     2461 atttaaatcc taaacaattt aaagaagcct tgcttgatga agatactgtt gttttagata
     2521 cgcgtaatga ttatgagtat gacttaggtc acttccgtgg agcaattcgt cctgatattc
     2581 gcaatttccg tgagttacca caatgggtac gtgacaacaa agataagttt atggaaaaac
     2641 gtgttgttgt gtactgtacc ggtggtgttc gttgtgagaa attctcgggt tggatggttc
     2701 gagaaggttt taaagatgtt ggacaattac acggtggtat tgcaacatat ggaaaagatc
     2761 cggaagttca aggtgaactt tgggatggtg cgatgtatgt ttttgatgat cgtatctcgg
     2821 tgccaattaa tcatgttaat ccaactgtta ttagtaaaga ttattttgat ggaacacctt
     2881 gtgaacgtta tgtcaattgt gcaaatccgt tttgtaacaa acaaattttt gcttcagaag
     2941 aaaacgaagc gaagtatgtt cgagggtgct cgcctgaatg tcgtgcacat gaacgtaatc
     3001 gctatgtgca agaaaatggt ttatcacgtc aagaatgggc agaacgttta gaagctattg
     3061 gagaatcctt acctcaagta gcaaatgttt aatattaaaa aatcgctagc aataaagcta
     3121 gcgatttttt agcaatcaca attgacatcg caacaagcta ggttatcaag gtacatttga
     3181 ttttctttaa agaattcaag ggtatcttcg tctaatataa tcccttctcg ttgtgcgata
     3241 tgattaataa cagggacaaa gcgctgtctg aatttataaa tatagcagaa aatagtatct
     3301 ctttgtcgta agcttggacc aattaagaaa cagttattaa cgatagtaga ttcgtcatct
     3361 tcagtaacta ttggcaactg atttttatcg tattcaaata aagcgattcc gtcaatgaga
     3421 tgacaggtat ttagaaaacc tgtggaaaga ataggtttat ggaaagactc cgcagtacta
     3481 ccgtcatcaa aagtaacttg atattgtttt ccaatttgtt taatctcaat ggctttcttg
     3541 ccttcagaga tactataata ttcttttttg tggtcttgta tatgttttaa acgttcttta
     3601 gttagggggg ataaagagat gcttggatcg ggattgctct ctttacgacc aaacgtatct
     3661 gtatataatt cgacttgatt tcccaaatat actagatgtg ttagtgcatc acaagcactt
     3721 tcgtttccac caatgatgat aaatggattg tcagatttga tatggaaatt atcaacttga
     3781 ccatagtgca ttcctagatc tgcaccttta atatcaatgg tatttggatt ttgaaattct
     3841 cctgttgcca taataagata atcagcagaa aaatctccct ttgaagtttt aataacaaaa
     3901 aggctatctt gtttatcaat agatagaaca gatgtttcgt tttggatagg gagattataa
     3961 tgagcagcaa ctaattgtaa gtagcgtgca tattccactc cagaaagatg ttctttttca
     4021 aatgagaaag caggagaagt atctggaata acagcgttta gatctggaaa accaaagcca
     4081 ttggtagtga aggaaggtgt gataaattgt gttgttctgg gccatcttaa aaaagattct
     4141 ccaatgtgcc ctttttcaat gataagaaaa tttgtaagtc ctaatcgttg catggcagaa
     4201 ccaaaaccga ttcctgcagc gcctgcaccg ataattatgg tttgataatg tttcatgtct
     4261 tctccttgac aaaaataaat gtgtaagata aaatacttaa ccagttaaca ataatacagt
     4321 ttaacaaata aaagagtgtt tgtaaagaaa ggactatttg tgtttttact attatgtatt
     4381 ttatcgatgt ggctcatgat ttttggggtt gcattctatt actttgggtc gctacatcaa
     4441 tcattgactt caaggattat ttatcagttt gtacttactg ttttgctaac gacaacagct
     4501 tggttcatgg gtgcctattt tttagcattt gaaggtcatt ttaaaactgt ctttcaattc
     4561 caagaagcag atggtaaaca aattgtaaat tgtttatttc aactttgttt cgccctttac
     4621 gcagtagtaa tgcttattgg gtcaattatt gatagagttc agacgaagcg acttttatta
     4681 gctgttgttt cttggctttt cttggtgtat acgcccttag cttatttaat ttggaattca
     4741 gagggtgttt ttgcaaagat gggagtactt gatttttcag gcggaatggt cgttcattta
     4801 tctgcagggc tttcttcgta tattttagct catgttattg gcaagagtga acatcagcac
     4861 aataaggtta aaaatgatag tctcttttta ggaatgatac ttattacatt tggttggttt
     4921 ggatttaata tgggtccagt tggtgagtgg aattcacaag ccattatgat acttttaaat
     4981 actatatttg ctattatagg tggtggtttg gcgtggacat tggctgctaa gtggaatggt
     5041 gaggaagaga aaacaggaag cttattgaat ggcattattg ttggtcttgt gacgagtaca
     5101 gcaggagttg ggtatctctt aacttggcaa ttattagctg taacattctt tgcatccctc
     5161 ttcacctact ttgtaacgga ttacgttgcg aaagcttttg ccattgatga tgtcgtttcc
     5221 tcctttggaa tgaatggtat tgggggattg ttgggaagtt taggagttgg tctctttaaa
     5281 ttatcacata tgccagttca actgttagca ttagctacca caattctttt gtcaattata
     5341 atgacctata tcatatctaa agcgattttt agaaaataat aaataaggtt gagatgtaca
     5401 tctcaacctt atttattatt agacttcctg cgaaacaaaa tatgatacaa tagttctatg
     5461 aattatgaag caagcaaaca attaactgat gtacgattta agcgccttgt tagtgttcag
     5521 cgcactactt ttgaagagat gttagctgtg ttaaaaacag cttatcaacg
//
LOCUS       DATRTN010000100         5516 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.100, whole genome shotgun sequence.
ACCESSION   DATRTN010000100 DATRTN010000000
VERSION     DATRTN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.100"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(102..575)
                     /locus_tag="VBE22_001766"
     CDS             complement(102..575)
                     /locus_tag="VBE22_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203198.1"
                     /translation="MIAIIRTREKGRSQFLCELDIMQFTENQVRNRMIEKGIKDDAFF
                     ICGFSDWNVDRIMSLTEVYLLKRCIEGLYDGDDYIVQYLLKKGLSVHSIVTKYYIFLS
                     NNENKVMKYILRKVEFDSLIDFWQRSVTWVNALNAYIEEGIVLNTSKGFYVLKTE"
     gene            complement(583..1065)
                     /locus_tag="VBE22_001767"
     CDS             complement(583..1065)
                     /locus_tag="VBE22_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000383283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO7203199.1"
                     /translation="MDVSAIVRDIKTGRATLVCFPVEMSELKLALGLREEDDLEYIIA
                     DSDCQLLKEHDSIEMINQFVELVENVDSELVKAVHQVIGYTASDFVDYDFNFGDCCLL
                     SDVTTRRELGEYYFDELGVQGVGKEALEMYFDHEAYGRDIDLESQGGFSDYGYVEISG
                     "
     gene            complement(1118..1390)
                     /locus_tag="VBE22_001768"
     CDS             complement(1118..1390)
                     /locus_tag="VBE22_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203200.1"
                     /translation="MSNTRSEADKKLLVVTQELSELLVSHQYEQSWEKAGELNSLLKK
                     REELTLPGYMVDMIQQHLKSYYYQNNMINKAHKSMSAIGHKLQEFH"
     gene            complement(1522..1875)
                     /locus_tag="VBE22_001769"
     CDS             complement(1522..1875)
                     /locus_tag="VBE22_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7203201.1"
                     /translation="MIENFALNLIRLRKQKGLSQKELAKKIGMLPQTISNIENQKGYP
                     TFSNLDKIARYFNVSATELFGTPQQIELERVMFQTDEYSRKAEKILSAVSVFENFVKK
                     YDYQKIIEFGLSLKE"
     gene            complement(1868..2098)
                     /locus_tag="VBE22_001770"
     CDS             complement(1868..2098)
                     /locus_tag="VBE22_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7203202.1"
                     /translation="MNVTDFKSNFIFPKRLKSLRQESGLKQQEIAPLLGIKQNTYSDW
                     ETGKSEPNLENVVKLAKLFKTTTDFLLGVTDD"
     gene            complement(2085..2462)
                     /locus_tag="VBE22_001771"
     CDS             complement(2085..2462)
                     /locus_tag="VBE22_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203203.1"
                     /translation="MYSNREFAYFVNRRNNLDKMIDLLVFMIPDREFYYPEIQTGELR
                     DYQIDIYDLIKIGYVGVYEIQKDYEDKLRELADFKRKLLKFGLLMQPLDRQKEIVMNL
                     ASQYRLHKRLLKLRENFRGDERD"
     gene            complement(2478..3710)
                     /locus_tag="VBE22_001772"
     CDS             complement(2478..3710)
                     /locus_tag="VBE22_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO7203204.1"
                     /translation="MDAIYLKKFRKKTGLKQKEFALSSGLTLKSLRNYEQGKRKLTLE
                     KYQEIKSHFGYLVENDSSRLQVMIDYVRITLKDVRDLEFFCRNFLHCAFKEFQPFESK
                     LMNYNHLWKRGDIWIFDFADKHETGNFQITVQLSGRGCRQLELLMETEKFTWHDWLSY
                     LRNSYRDDMNVTRFDIAIDELYLGKDRENEQFHLSDMISKYYRHELDFESLRTWNYIG
                     GGSLNFSDMEEIEQNRQGISLYFGSRQSEMYFNFYEKRYEIAKQEGITVEEALEIFEL
                     WNRYEIRLSQSKANAAVDEFISGVPIGEISRGLIVSKIDVYDGKNEYGSFQADRKWQL
                     MFGGVEPLKFVTKPEAYSIERTLRWLSDSVSPSLAMIREYDMIVDGDYLQTILNSGEV
                     NERGEKILDSIKASLGIL"
     gene            complement(3998..>5516)
                     /locus_tag="VBE22_001773"
     CDS             complement(3998..>5516)
                     /locus_tag="VBE22_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO7203205.1"
                     /translation="VSWYLTWFLQEANPLFNKLDRLKRMSKFLYENGYVYEKRKKSNK
                     KTKTKYRFPKVYVKQGKYDLSVSFEMAGGKFQKKFKDIGGELEDTFFMDFMEKTDDPR
                     FKIYKLAYSAFLSRITVKDVIWDKDKGIKLMDGYYWDFINDPHLLVAGGTGGGKTVLL
                     RSILRCLAEIGVCDICDPKRADFVTMSDLSAFEGRIAFEKADIIEKFENAVTIMFARY
                     DFVRNEMKRLGHKDMKKFYDYGLEPYFFVCDEYNALMSSLSYQEREIVDNAFTQYILL
                     GRQVGCNAIIAMQKPSADDLPTKIRSNMMHHISVGRLDDGGYVMMFGDENRNKEFRFI
                     KYLAGRRVYGRGYSAVFGEVAREFYSPLLPKNFSFYDAFEKINRHENPFDPTENQEVS
                     KAILKDESLREFVEKTSENELLKGSVGFDFDDEMEEMENMSIGDLAKKTGFSFGKIKN
                     LIDMIEKDNYMTFSREDEKVVLNDSDAVVLESLLTQKDEFEGSWKELLLNYFKGE"
BASE COUNT         1845 a         1170 c          604 g         1897 t
ORIGIN      
        1 ttgacaacat gttgtacaag ttttggtttt gataatcact aaataaataa cttacaaatg
       61 ataacaatac ataaattatc gttaagcttc caattacctc ctcattctgt ttttaacacg
      121 taaaaacctt ttgaggtgtt taatactatt ccttcttcaa tataagcatt taaagcattt
      181 acccaagtta ctgaccgttg ccaaaaatca attaaagaat caaattcaac tttccttaag
      241 atatatttca ttactttatt ttcattattt gacaaaaata tataatattt tgtcactatt
      301 gaatgaactg ataaaccttt tttcaaaaga tattgaacaa tatagtcatc accatcataa
      361 agtccttcaa tacatctttt taaaagatag acttctgtaa gtgacataat cctatcaaca
      421 ttccaatcag aaaaaccaca aataaaaaag gcatcatcct taatgccttt ttctatcata
      481 cggtttctta cttgattttc cgtaaactgc atgatgtcta actcacataa aaattgactt
      541 ctaccttttt cacgagtcct tataattgca atcattatct acttaccctg atatttcgac
      601 atatccgtag tctgaaaaac caccttggct ttctaagtca atatctcgac cataagcttc
      661 atgatcaaaa tacatctcca gagcctcttt accaactcct tgtacgccta attcatcgaa
      721 ataatattca cctagttctc gtctagttgt gacatcagac agtaaacaac aatcaccaaa
      781 attaaaatca taatccacaa agtctgaggc agtataacct attacctgat gcactgcttt
      841 aacaagttca ctatcaacat tctctaccag ttcaacaaat tgattaatca tctcaataga
      901 atcatgttct tttagtaact gacaatccga atctgcaata atatactcta aatcatcttc
      961 ttcacgaagc cccaaagcta acttaagctc actcatttcc acaggaaaac aaactaacgt
     1021 agctctacca gtttttatat ctctaacaat tgcactaaca tccatttttg acactcctta
     1081 attaaaaaag gagcaagcca ttgacttgct ctcaccctta atgaaattct tgtagtttat
     1141 gaccaatcgc tgacatagac ttatgagcct tattaatcat attattttgg tagtagtatg
     1201 atttcaaatg ttgttgaatc atgtcgacca tataacctgg taaagtcaat tcttcacgct
     1261 ttttaagtaa gctatttaac tctccagctt tttcccatga ctgttcatac tgatggctga
     1321 ccaacagctc tgaaagctcc tgagtaacca ctaacaactt tttatctgct tctgaacgtg
     1381 tatttgacat ttttcttttc ctctttttct attgaatatt aataatttac aaattgtttc
     1441 taactttagt tggacttcta ataaacataa tttcctccca aaataaaaag agtaggggta
     1501 gcctacttac ttttgtattt tttattcttt taaactcaaa ccaaattcta taatcttttg
     1561 ataatcatat ttctttacaa aattttcaaa aaccgaaaca gcagacaaaa tcttttctgc
     1621 tttacggcta tattcatcag tttgaaacat tactctttct aattcaattt gttgtggtgt
     1681 tccaaaaagt tccgttgccg agacattgaa atatcgagca attttatcca agtttgaaaa
     1741 tgtaggatag cctttttgat tttcaatatt tgaaatggtt tgcggtaaca ttcctatttt
     1801 tttggcaagt tctttttgag atagaccttt ttgtttgcgt aaacgaatta aattaagtgc
     1861 aaaattttca atcatctgtt acccccaata aaaaatctgt agttgtttta aataattttg
     1921 ctagcttgac gacattttct aaattcggtt cacttttccc tgtttcccaa tcactataag
     1981 tattttgttt tatccctagt agtggagcta tctcttgctg ttttaatcca ctttcttgcc
     2041 ttaaactttt aagtctcttt ggaaatataa aattagattt gaagtcagtc acgttcatca
     2101 cctctaaaat tttctcttag ctttaataat cttttatgta aacgatactg actagctaaa
     2161 ttcataacaa tttctttttg cctatctagt ggttgcatga gtaacccaaa ctttaaaagt
     2221 ttccgtttaa aatcagcaag ttcccttaac ttatcttcgt agtctttttg aatttcataa
     2281 acaccaacat aaccaatctt aatcaaatca taaatgtcta tctgataatc tctaagctca
     2341 cctgtttgaa tctcaggata ataaaactca cgatcaggaa tcataaaaac tagtaaatca
     2401 atcattttat ctaaattatt tcgtctatta acaaaataag caaattctcg gttactatac
     2461 aaaacaaaac ttacctccta caaaatacct aagctagctt tgatactgtc aagtatcttc
     2521 tccccacgtt cattgacttc cccagaatta agaatggttt gtaaatagtc accatccaca
     2581 atcatgtcat actctcttat catagctaaa gacggagaaa cagaatcact taaccaacgt
     2641 aaggttcgtt caatactata agcctctggc tttgtaacaa attttaaagg ctcaacacct
     2701 ccaaacatta attgccactt tctatctgct tgaaacgacc catattcatt tttcccatca
     2761 tacacgtcaa tcttactaac aattaaccct ctggatattt caccaatcgg cactccagaa
     2821 ataaattcat caactgccgc attagcttta ctttgagata accgtatctc ataacgattc
     2881 cacaattcaa aaatttctaa agcttcttca acagtaattc cttcctgctt tgcaatttca
     2941 taacgttttt cataaaagtt aaaatacatt tctgattgac gactaccaaa ataaagcgat
     3001 attccttgtc tattctgctc aatctcttcc atatccgaaa aatttaaaga accgccacct
     3061 atataattcc aagttctcaa actttcaaaa tctaactcat gacgataata ttttgaaatc
     3121 atatctgaca aatgaaactg ttcattttct ctatccttac ccagatacaa ttcatcaata
     3181 gcaatatcaa aacgtgtcac attcatatca tcacgatatg aatttcttag ataacttaac
     3241 caatcatgcc atgtaaattt ttcagtttcc atcaacaatt ccaattgtcg gcatccacga
     3301 ccagacaatt gaacagttat ttgaaaatta cctgtctcat gtttatccgc aaaatcaaat
     3361 atccaaatat ctccacgttt ccaaagatga ttgtaattca tcaacttcga ttcaaaaggc
     3421 tgaaattctt taaatgcaca gtgcaaaaag ttacgacaga aaaattcaag atccctaaca
     3481 tcctttaacg taattctgac atagtcaatc atcacttgta atcgtgaact atcattttct
     3541 actagatatc caaaatgtga ttttatctct tgatactttt caagtgtcaa cttccgcttt
     3601 ccttgctcat agtttctaag tgatttcaat gtcaaaccag aacttaaagc aaattccttt
     3661 tgttttagac ctgttttctt tcgaaatttt tttaaataga tagcatccac aataaacaaa
     3721 ctccttctta ggattagtga cccacttttt tcactaaaat gacccacctt taaaaatctg
     3781 aaaacccttg atacatatag gtttttgggg gttaaaattc gaaatttttc atttcttacc
     3841 cccctattag aaaacggggg ttttaaaatt gataaatccc tctgtaaagc cctagtgtgt
     3901 gcgctgtgtc cggcgtgtcc gttttcgcta cgctacacta cgcctacagc tacactaggg
     3961 ctttacagaa ggatatttaa aaaataccct tcatctacta ttcaccctta aaataattca
     4021 atagtaactc tttccagcta ccctcaaatt catctttttg tgtcagtaaa ctttctaaga
     4081 cgactgcatc agaatcattt aatacaacct tttcatcttc acgactaaaa gtcatataat
     4141 tatctttttc aatcatatct attaaattct taatcttacc aaatgaaaaa cctgttttct
     4201 tagctaaatc accaatactc atattttcca tttcctccat ttcatcatca aaatcaaacc
     4261 ctactgaacc cttaagtaac tcattttcag acgttttttc cacaaactct ctcaaagatt
     4321 cgtctttaag aatcgcctta gaaacttcct gattttctgt aggatcaaaa ggattctcat
     4381 gcctatttat tttttcaaaa gcatcataaa aagagaaatt tttaggtaat aaaggagaat
     4441 aaaattctct tgctacttca ccaaaaacag ctgaataacc tcgaccataa acacgacgac
     4501 ctgcaagata tttaataaac ctaaattcct tatttctatt ttcatcacca aacatcatca
     4561 cataaccacc atcatcgaga cgaccaaccg agatatggtg catcatatta gatctaattt
     4621 tagtgggaag atcatctgca gacggctttt gcattgctat aatcgcatta catccaacct
     4681 gacgtcctaa caatatatat tgagtaaatg cattatctac aatctcacgt tcctgataag
     4741 acaaagaact cattaaagca ttatattcat cacatacaaa aaaataaggt tccaatccat
     4801 aatcataaaa ctttttcatg tctttatgac ctaaacgctt catttcattt cgaacaaaat
     4861 cataacgtgc aaacattatc gtaacagcat tttcaaactt ctcaataata tcagcctttt
     4921 caaaggcaat tctaccttca aaagctgata aatctgacat agtaacaaaa tcggctcttt
     4981 tagggtcaca tatatcacaa actccaattt ccgcaagaca acgaaggatt gaccttagta
     5041 atactgtttt acctccacca gtaccaccag caactaataa atgaggatca ttgatgaaat
     5101 cccaataata gccatccatt aacttaattc ccttatcttt atcccatatc acatctttaa
     5161 ccgtaatacg tgataagaaa gccgaataag ctaatttgta aattttaaag cgtggatcat
     5221 cagttttttc cataaaatcc atgaaaaaag tatcttctaa ttctccacca atatccttaa
     5281 atttcttttg aaattttcct ccagccattt caaatgaaac acttaaatca tacttacctt
     5341 gtttcacata aacttttgga aaacgatatt ttgtcttcgt tttcttattt gattttttac
     5401 gtttttcata aacatagcca ttctcatata aaaacttaga cattcttttt aatcgatcta
     5461 gtttattaaa taatggatta gcttcttgca aaaaccaagt caaataccaa gaaaca
//
LOCUS       DATRTN010000101         5360 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.101, whole genome shotgun sequence.
ACCESSION   DATRTN010000101 DATRTN010000000
VERSION     DATRTN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.101"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..2205
                     /locus_tag="VBE22_001774"
     CDS             <1..2205
                     /locus_tag="VBE22_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO7203206.1"
                     /translation="KKVKYFLRKTAYGLASMSAAFAVCSGIVHADTSSGISASIPHKK
                     QVNLGAVTLKNLISKYRGNDKAIAILLSRVNDFNRASQDTLPQLINSTEAEIRNILYQ
                     GQIGKQNKPSVTTHAKVSDQELGKQSRRSQDIIKSLGFLSSDQKDILVKSISSSKDSQ
                     LILKFVTQATQLNNAESTKAKQMAQNDVALIKNISPEVLEEYKEKIQRASTKSQVDEF
                     VAEAKKVVNSNKETLVNQANGKKQEIAKLENLSNDEMLRYNTAIDNVVKQYNEGKLNI
                     TAAMNALNSIKQAAQEVAQKNLQKQYAKKIERISSKGLALSKKAKEIYEKHKSILPTP
                     GYYADSVGTYLNRFRDKQTFGNRSVWTGQSGLDEAKKMLDEVKKLLKELQDLTRGTKE
                     DKKPDVKPEAKPEAKPNIQVPKQAPTEAAKPALSPEALTRLTTWYNQAKDLLKDDQVK
                     DKYVDILAVQKAVDQAYDHVEEGKFITTDQANQLANKLRDALQSLELKDKKVAKPEAK
                     PEAKPDVKPEAKPDVKPEAKPDVKPEAKPDVKPEAKPDVKPEAKPDVKPKAKPDVKPE
                     AKPDVKPDVKPDVKPEAKPEAKPDVKPDVKPEAKPDVKPEAKPEAKPEAKPEAKPEAK
                     PEAKPEAKPDVKPEAKPDVKPEAKPEAKPEAKSEAKPEAKLEAKPEAKPATKKSVNTS
                     GNLAAKKAIENKKYSKKLPSTGEAASPLLAIVSLIVMLSAGLITIVLKHKKN"
     gene            2411..2950
                     /gene="nusG"
                     /locus_tag="VBE22_001775"
     CDS             2411..2950
                     /gene="nusG"
                     /locus_tag="VBE22_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEO7203207.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGKTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRSILISMGQTVDVFDTNIKEGDVVQIIDGAFIGQEGRVVEIENNKVKLMI
                     NMFGSETQAELELYQVAEL"
     gene            complement(2997..4202)
                     /locus_tag="VBE22_001776"
     CDS             complement(2997..4202)
                     /locus_tag="VBE22_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 8 glycosyl transferase"
                     /protein_id="HEO7203208.1"
                     /translation="MEKAVALAVDSNYLDKALVTIKSICVYNRNITFYLFNQDTPVEW
                     VRNINRKLEPLGSKLINVKIYNYDIAHLTTFLTVSTWFRLFLADYIPSSRVLYLDSDI
                     IVNTNLDYLFELDFKGYYLAAVKDPHKNEEGGFNAGMLLANLELWREDGLTKTLLKTA
                     EELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQTYDVVSRYNHRSYLYLNIENRTPNI
                     IHFLTSDKPWNENSVARFRELWWYYFQLDFCQLTGKQRKVISYEKSMELLSVSDIHLF
                     TLTSSDNLEHIESLICRCPTVQFHIGAYTTVSNKLSKLEQYPNVLVYPELIEARIEKL
                     ITLATAYLDINHGPEVGNILQRVHLKQKPIYSFNNTSHQENITKHIVNHNSIDNMVVL
                     INELNLNQL"
     gene            complement(4267..>5360)
                     /locus_tag="VBE22_001777"
     CDS             complement(4267..>5360)
                     /locus_tag="VBE22_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEO7203209.1"
                     /translation="FIFNQDIPQEWFLAMKDRVGQTGNQIQDVKLFHDHLSPKWENKK
                     LNHINYMTYARYFIPQYISADTVLYLDSDLVVTTNLDNLFQISLDNAYLAAVPALFGL
                     GYGFNAGVMVINNQRWRQENMTIKLIEKNQKEIENANEGDQTILNRMFENQVIYLDDT
                     YNFQIGFDMGAAIDGHKFIFDIPITPLPKIIHYISGIKPWQTLSNMRLREVWWHYNLL
                     EWSSIISSKKVFGLDHPIKTQNYRLNFLIVTTSDCIPSISELVTALPDCLFHIAAPTV
                     MSDILISLLAFENIRLHKTYTPPIIDKLLAEADIYLDINVFGKMEGILQKMSQSEKPI
                     LTTRECNSQLVPETVFASIDDLINYLQSI"
BASE COUNT         1956 a          856 c          972 g         1576 t
ORIGIN      
        1 aaaaaagtaa aatacttttt aagaaaaaca gcttatggtt tggcctcaat gtcagcagcg
       61 tttgctgtat gtagtggtat tgtacacgcg gatactagtt caggaatatc ggcttcaatt
      121 cctcataaga aacaagttaa tttaggggcg gttactctga agaatttgat ttctaaatat
      181 cgtggtaatg acaaagctat tgctatactt ttaagtagag taaatgattt taatagagca
      241 tcacaggata cacttccaca attaattaat agtactgaag cagaaattag aaatatttta
      301 tatcaaggac aaattggtaa gcaaaataaa ccaagtgtaa ctacacatgc taaagttagt
      361 gatcaagaac taggtaagca gtcaagacgt tctcaagata tcattaagtc attaggtttc
      421 ctttcatcag accaaaaaga tattttagtt aaatctatta gctcttcaaa agattcgcaa
      481 cttattctta aatttgtaac tcaagccacg caactgaata atgctgaatc aacaaaagct
      541 aagcaaatgg ctcaaaatga cgtggcctta ataaaaaata taagccccga agtcttagaa
      601 gaatataaag aaaaaattca aagagctagc actaagagtc aagttgatga gtttgtagca
      661 gaagctaaaa aagttgttaa ttccaataaa gaaacgttgg taaatcaggc caatggtaaa
      721 aagcaagaaa ttgctaagtt agaaaattta tctaacgatg aaatgttgag atataatact
      781 gcaattgata atgtagtgaa acagtataat gaaggtaagc tcaatattac tgctgcaatg
      841 aatgctttaa atagtattaa gcaagcagca caggaagttg cccagaaaaa cttacaaaag
      901 cagtatgcta aaaaaattga aagaataagt tcaaaaggat tagcgttatc taaaaaggct
      961 aaagaaattt atgaaaagca taaaagtatt ttgcctacac ctggatatta tgcagactct
     1021 gtgggaactt atttgaatag gtttagagat aaacaaactt tcggaaatag gagtgtttgg
     1081 actggtcaaa gtggacttga tgaagcaaaa aaaatgcttg atgaagtcaa aaagctttta
     1141 aaagaacttc aagaccttac cagaggtact aaagaagata aaaaaccaga cgttaagcca
     1201 gaagccaaac cagaggccaa accaaatatt caagtaccta aacaagcacc tacagaagct
     1261 gcaaaaccag ctttgtcacc agaagccttg acaagattga ctacatggta taatcaagct
     1321 aaagatctgc ttaaagatga tcaagtaaag gacaaatacg tagatatact tgcagttcaa
     1381 aaagctgttg accaagctta tgatcatgtg gaagagggaa aatttattac cactgatcaa
     1441 gcaaatcaat tagctaacaa gctacgtgat gctttacaaa gtttagaatt aaaagataaa
     1501 aaagtagcca aaccagaagc caaaccagag gccaaaccag acgttaagcc agaggccaaa
     1561 ccagacgtta agccagaagc taagccagac gttaaaccag aagctaagcc agacgttaaa
     1621 ccagaagcta agccagacgt taaaccagaa gctaagccag acgttaaacc aaaggccaaa
     1681 ccagacgtta agccagaagc taagccagac gttaaaccag acgttaaacc agacgttaag
     1741 ccagaggcca aaccagaggc taagccagac gttaaaccag acgttaagcc agaagctaaa
     1801 ccagacgtta agccagaggc caaaccagaa gctaagccag aagctaagcc agaagctaag
     1861 ccagaagcta agccagaggc caaaccagaa gctaagccag acgttaagcc agaagctaaa
     1921 ccagacgtta aaccagaggc taagccagaa gctaaaccag aggctaagtc agaagctaaa
     1981 ccagaggcta agctagaagc taaaccagag gccaaaccag caaccaaaaa atcggttaat
     2041 actagcggaa acttggcggc taaaaaagct attgaaaaca aaaagtatag taaaaaatta
     2101 ccatcaacgg gtgaagccgc aagtccactc ttagcaattg tatcactaat tgttatgtta
     2161 agtgcaggtc ttattacgat agttttaaag cataaaaaaa attaaattta tttttaaaca
     2221 taaaccgaaa ggtctatcct ttcaaaatct taaggcaaac ctcatcagaa ctttgccttt
     2281 tttatttgaa ttttagaatg atttagtcgg tactttaatt gacagcttat tttattttct
     2341 ttataatata tattgaaaat agcagtccaa taagccgtaa aaggcttttt tattaaggaa
     2401 aggaaaactt atgttagatt cttttgataa gggttggttt gtactccaaa cctactcagg
     2461 ttatgaaaat aaagtaaagg aaaatctctt gcaacgtgcg caaacttata atatgttaga
     2521 caacattttg cgtgtggaaa tccctactca aactgtaaat gttgagaaaa atgggaaaac
     2581 aaaagaaatt gaagaaaatc gcttcccggg ttatgtactt gtggaaatgg taatgactga
     2641 tgaagcctgg tttgttgttc gtaatactcc aaacgttact ggatttgttg gatcacacgg
     2701 caatcgctct aaaccaacac cgttgttaga agaagagatt cgttcaatac tcatctcaat
     2761 gggacaaacc gttgatgtct ttgatactaa tatcaaggaa ggtgatgttg ttcaaatcat
     2821 tgatggtgct ttcatcggac aagaaggacg tgtagttgag attgagaata acaaagttaa
     2881 attaatgatc aacatgttcg gctcagaaac acaagcagaa cttgagttgt accaagttgc
     2941 agaattataa aacttctgaa aaatccatct taaaataaag atggattttt gttaatttat
     3001 aattgattta ggtttaattc gttaattaaa acaaccatat tatcaataga attatgatta
     3061 acgatatgtt tggttatatt ctcttgatga gacgtgttgt tgaaagaata aattggcttt
     3121 tgtttcaaat gaactctttg taaaatatta cctacttctg gaccatgatt aatatcaagg
     3181 tatgcagtgg ctaatgttat taatttttca attcttgcct caattaattc agggtaaact
     3241 aggacatttg gatattgttc tagtttgcta agtttatttg acactgttgt gtaggcacca
     3301 atatggaatt gaacagtagg acatctacaa attagcgatt caatgtgttc taaattatcg
     3361 gaagatgtaa gagtgaaaag atgaatatct gaaacagaaa gcaattccat ggacttttcg
     3421 taagaaatca cttttctttg cttgccggtt aattggcaaa aatcaagttg gaagtaatac
     3481 caccatagtt ctctaaacct tgcaacgcta ttttcattcc aaggtttgtc actagttaaa
     3541 aaatgtataa tattaggagt tctattttct atgtttagat ataaataaga tcgatgatta
     3601 tagcggctaa cgacatcata agtttgaaag ttccatgttt tgttcagaga taaccaacga
     3661 ttatggcaaa caatgttcaa gatactttga tcccctgttt tgacaactcg gtggagttct
     3721 tcagctgttt ttagtaatgt tttagtgagc ccatcttccc gccatagttc tagattagct
     3781 aaaagcatgc cagcattaaa ccctccttct tcatttttat ggggatcttt gacggctgct
     3841 aagtaataac ctttaaaatc tagttcaaat aagtaatcaa gattagtgtt aacgataata
     3901 tctgaatcta aataaagtac acgtgaacta ggtatataat ctgctaaaaa taatctaaac
     3961 catgtactaa cagttagaaa agtcgttaga tgagcaatat catagttata tattttaaca
     4021 ttaatcagtt ttgatcctag aggctctagt ttcctgttta tattacgtac ccattcaact
     4081 ggggtatctt gattgaataa ataaaaagtt atatttctat tataaacaca aatagacttt
     4141 attgttacta aggctttatc caagtagttt gaatcaactg caagtgcaac tgctttttcc
     4201 atttaaattc tcctagttaa gtgtttagaa taatgttggg tatatagtga atggatgcca
     4261 atttttttat atagactgaa gataattaat taaatcatcg atagatgcaa aaactgtttc
     4321 tggaacaagc tggctattgc attctcttgt agtaagaatt ggtttttcag attgtgacat
     4381 tttctgtaga atgccctcca tttttccaaa aacattaata tcaagataaa tatcagcttc
     4441 tgctaataat ttatcaataa taggtggagt gtaagtttta tgaaggcgga tattttcaaa
     4501 agctaatagg cttataagaa tgtcagacat aactgttggt gctgcaatgt gaaatagaca
     4561 atctggaagg gcagtgacta attctgagat agatggtata caatcagaag ttgtaacaat
     4621 aaggaaattg agacgataat tttgtgtttt aattgggtgg tctaaaccaa atactttttt
     4681 actagatatg atacttgacc attcaagtaa attatagtgc caccatacct cacggagtct
     4741 catatttgat aatgtttgcc aaggtttgat tcccgaaatg tagtgaataa tttttgggag
     4801 tggggtaatt gggatgtcaa aaataaattt atgcccatcg atagcagctc ccatatcaaa
     4861 accaatttga aaattgtagg tatcatctaa ataaattacc tgattttcaa acatgcgatt
     4921 aagaattgtt tgatcccctt cgttggcatt ctcaatttcc ttttgatttt tttcaattaa
     4981 tttaatagtc atattttctt gtcgccaacg ttggttgtta attaccatta ctccagcatt
     5041 aaacccatat ccaagcccaa aaagagctgg aactgcagct aaatatgcat tgtctagtga
     5101 aatttgaaag aggttatcta aattagtagt aacaactaag tcagagtcaa gatataaaac
     5161 tgtatcagct gagatgtact gaggtatgaa ataacgagca taggtcatat aattaatatg
     5221 attaagcttt ttattttccc attttgggga taagtgatca tggaagagct ttacatcctg
     5281 aatttgattt ccagtttgtc caaccctatc tttcatagct aaaaaccatt cttgaggaat
     5341 atcttgatta aaaataaaaa
//
LOCUS       DATRTN010000102         5359 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.102, whole genome shotgun sequence.
ACCESSION   DATRTN010000102 DATRTN010000000
VERSION     DATRTN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.102"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..1372
                     /locus_tag="VBE22_001778"
     CDS             <1..1372
                     /locus_tag="VBE22_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEO7203210.1"
                     /translation="IIDHLHVVGDIYDRGRYPDRILNRLMAFPNLDIQWGNHDVTWMG
                     AASGSYLCMVNVIRIAARYNNITLIEDRYGINLRRLVDYSRRYYEPLPSFAPILDGEE
                     MTHPDELDLLNMIQQATAILQFKLEAQLIDRRPEFQMHNRQLINQVNYKDLSISIKGV
                     VHQLKDFNSRCIDSKNPSRLTSEEEELLQQLMIAFQTSESLKKHIDFLFEKGSMYLTY
                     NDNLLFHGCIPMHSNGDFKSFKIAGKTYGGRDLLDLFESQIRLAYARPEKHDDLATDI
                     IWYLWCGENSSLFGKNAMTTFERYYVSDKVTHQERKNPYFKLRDKDDICTTLLQEFDL
                     PKFGHIVNGHTPVKEKNGEQPIKANGKMLVIDGGFAKGYQKNTGLAGYTLIYNSYGIQ
                     LISHLPFTSIEEVLSGTNYIIDTKRLVEEAKDRILVKDTTIGQKLTKEIKDLDHLYRH
                     FQEYDD"
     gene            1462..2586
                     /gene="queG"
                     /locus_tag="VBE22_001779"
     CDS             1462..2586
                     /gene="queG"
                     /locus_tag="VBE22_001779"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEO7203211.1"
                     /translation="MDIKAEIQKLAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPNSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPLATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILEFMSNLTLKDEDSRKELELIRHKWQF"
     gene            2655..3753
                     /gene="prfB"
                     /locus_tag="VBE22_001780"
     CDS             join(2655..2726,2728..3753)
                     /gene="prfB"
                     /locus_tag="VBE22_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEO7203212.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENQMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            3772..4464
                     /gene="ftsE"
                     /locus_tag="VBE22_001781"
     CDS             3772..4464
                     /gene="ftsE"
                     /locus_tag="VBE22_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEO7203213.1"
                     /translation="MALIEMSGVTKKYRRSTTALRNLNLSIQQGEFVYLVGPSGAGKS
                     SLIRLLYREEKLSSGRLKVGEFNLNKLKRRQIPILRRSIGVVFQDYKLLPTKTVYENV
                     AFAMQVIGAKRRHIKKRVPEVLELVGLKHKMRSFPTQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEIAWEIMHLLERINLQGTTVLMATHNSQIVNTLRHRVIEIEAGSVI
                     RDEEKGEYGYHD"
     gene            4448..>5359
                     /locus_tag="VBE22_001782"
     CDS             4448..>5359
                     /locus_tag="VBE22_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7203214.1"
                     /translation="MVIMINFFRHFWESLKNLKRNFWMTFASVTSVTITLLLVGLFSS
                     VLLNVEKLTTDVSGNFTISAFLNVDSTDAQKQVKDKDGKLKDNPDYHKVYDKIKRISG
                     VEKVTYSSKAEQLKEVQKEYGSDVIDDTYKDALLDVYVVGTSSAKVSKNVSEAIGRIE
                     GVDYTKEPIDSTKLSNLTDNIRIWGFGGVALLIVLAIFLISNTIRMSIMSRRTDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGILGAIVPSLIFYFGYQFVFNKFNPKFETSHVSLYPM
                     DIMVPAIIGGMVIIGIIIGSLGSVLSMR"
BASE COUNT         1760 a          816 c         1097 g         1686 t
ORIGIN      
        1 aatcattgac catttacatg ttgtagggga tatttacgat cgtggtagat atcctgatcg
       61 tatcttgaat aggttaatgg cttttccaaa tttggatatc caatggggga accatgatgt
      121 cacttggatg ggagcggcat ctggttccta cctttgtatg gtaaatgtta tccgtattgc
      181 tgcacgttat aataatatta ctttaattga agaccgatat ggtatcaatt tgcgtcgctt
      241 ggttgattac tcacgccgtt actatgaacc tctaccttct ttcgctccta tattagatgg
      301 agaggaaatg actcatccag atgaactaga cttgttaaat atgatacaac aagcaacagc
      361 aattttacag tttaaattgg aagcccaatt gattgacagg cgtccagaat ttcaaatgca
      421 taatcggcaa cttattaacc aggtaaatta taaagattta agtatatcta taaagggagt
      481 tgtgcatcaa cttaaagatt ttaatagtag atgcattgat tcgaaaaatc ccagtcgatt
      541 aacaagtgag gaagaagagc ttttacaaca attgatgata gccttccaga cttctgaaag
      601 tttaaagaaa catattgatt tcctatttga aaaaggaagt atgtatttaa cctataatga
      661 taatctttta ttccatggtt gtataccaat gcatagtaat ggtgatttta aaagctttaa
      721 aattgcagga aaaacttatg gtggtaggga tttactggat ttatttgaaa gtcaaatacg
      781 tctagcatat gctagaccag agaaacatga tgatttggcg acagatatca tttggtatct
      841 ttggtgtggt gaaaattctt ctctttttgg taagaatgcc atgactactt ttgaacgcta
      901 ttatgtttcc gacaaagtaa ctcatcaaga aagaaaaaat ccctacttta aactgagaga
      961 taaagatgat atttgcacta ctcttttaca agagtttgat ttgcctaagt ttgggcatat
     1021 tgtcaatggt catacgccag tcaaagaaaa aaatggtgag caaccgataa aagcaaatgg
     1081 taagatgtta gtaattgatg gtggctttgc caaaggttat cagaaaaata caggtttagc
     1141 tggctacact ttgatttata atagctatgg gatacaatta atttcacacc ttccttttac
     1201 atccattgag gaagttttaa gtggaaccaa ctatatcatt gatacgaagc gattagtaga
     1261 agaagccaag gataggattc ttgtaaaaga tacaactatt ggacaaaagt taaccaaaga
     1321 gattaaagac ttagatcacc tttatcgtca ttttcaggaa tatgatgact agtgtttaag
     1381 taatttacta attgatatgt aggatttaat ttttcagttt cttatcttgt ctcttttatt
     1441 aaattgactt aaaataatat tatggacata aaggcagaaa ttcaaaaatt agcaaaagaa
     1501 attggtatct ctaaaattgg ttttacgacg gcagataact ttgattattt ggaaaagtca
     1561 ttgcgtgctt ctgtggaaga aggtcgcaat tcaggatttg aacataaagt catagaggat
     1621 cgtatctacc ctgaaaggct tttagaatcg gctaaaacga ttatttcaat tggcgttgct
     1681 tatcctcaca aacttcctca gcaacctcaa aaaacacctt ataaacgagg aaaaattaca
     1741 cctaattcgt gggggcttga ttatcattat gtggtcggcg aaaagttaga cagactatca
     1801 aaaggcattg aagagctctg tcgagatttt cctttgcaac aaaaagcaat ggtcgatact
     1861 ggagctttag ttgatactgc agttgcccaa agagcaggta ttggttttat aggaaaaaat
     1921 ggtctagtta tttctaaaga atatggctct tatatgtttt tgggagaatt aattaccaat
     1981 ttagagatcg agccagacaa gcctgtcgat tatgattgtg gtgactgtcg tcgttgtcta
     2041 gatgcctgtc caacttcttg tttaattggt gatggctcaa tgaatgccaa gcgttgtcta
     2101 tcatttcaaa cccaagataa gggaatgatg gacatagagt ttcgaaaaaa gattaaaaca
     2161 gttatctatg gttgtgatat ttgccaaatt tgctgtcctt ataacaaagg tataaataat
     2221 ccattggcga cagagattga tcctgagttg gctcaaccag aattaatacc atttttgagt
     2281 ttatcaaatg gacaatttaa agaaaaattt ggtatgattg ctggaagttg gcgaggtaaa
     2341 aatattttac agcgtaatgc cattattgcg ctagcaaatg ctcatgataa aaccgcagtg
     2401 gtcaaattaa tagagattat tgataaaaat aataacccta ttcatactgc aacagctata
     2461 tgggcattgg gagaaattgt caaaaaacca aacgatgaga tactggagtt tatgagtaat
     2521 ttaaccttaa aagatgaaga tagccgaaaa gagttggaat taatccgtca taaatggcaa
     2581 ttttaagcct tttatggtat ttttaagaac cttatgataa aataaaacag attaacaaag
     2641 attggagaaa tctcatggaa gtggctgaaa ttcgccaaaa aatagtagaa aataaagaga
     2701 agttgactag cttcaggagg tctctttgac ttagatcgtt tggaagagga gattgctctt
     2761 cttgaaaatc aaatgactga gccagacttt tggaatgaca atattgcagc gcaaaagaca
     2821 tctcaagaac tgaatgagtt aaaaggtaag tatgatactt tccataatat gcaagaatta
     2881 tctgacgaaa cagaactctt acttgagatg cttgacgaag atgattcttt gaaagaagaa
     2941 ttagaagaaa atctaatgca gctagataag ataatgggtg cttatgaaat gacgcttctc
     3001 ttatctgagc cttatgacca caataatgct atcttagaaa tccatcctgg ttcaggagga
     3061 actgaagcgc aagattgggg agatttactc ttacgaatgt atacccgttt tggcaatgct
     3121 aatggtttca aagtagaagt attggattat caagcagggg atgaagctgg tattaaatcg
     3181 gttaccctct cttttgaagg tcccaatgct tatggtttgt tgaaatcaga aatgggagtt
     3241 catcgcctcg ttcgtatatc accttttgat tcggctaaac gtcgtcatac ctcatttgct
     3301 tcagttgaag tgatgccaga gttagatgat acgattgaag tagaagtacg tgatgatgac
     3361 attaaaatgg atactttccg ttccggagga gctggtggtc agaacgtcaa taaagtatca
     3421 acaggtgttc gtttgacgca tattccaact ggaattgtgg tatcgtctac cgttgaccgt
     3481 actcaatatg ggaaccgcga tcgcgctatg aagatgttgc aggctaagct atatcagtta
     3541 gaacaagaaa agaaagcaca agaagtagat gctctcaagg gtgataaaaa agaaatcact
     3601 tggggaagtc agattcgttc ttatgttttc accccttata cgatggtgaa ggatcatcgt
     3661 actaactttg agcttgctca ggtagataag gttatggatg gagaaatcaa tggctttatt
     3721 gatgcctacc ttaagtggcg cattgaagac taaaaataat gaaggagaaa catggcacta
     3781 atagaaatgt ctggtgttac taaaaagtat cgccgttcaa caactgcctt gagaaactta
     3841 aatttatcaa ttcagcaagg tgagtttgtg tatttagttg gtccgtcagg tgctggtaaa
     3901 tctagcttga ttcgcctcct ataccgtgaa gaaaaattaa gttcaggtag attaaaggta
     3961 ggggaattca atctcaataa actcaaacgt cgtcaaattc ctattttacg tcgtagtatt
     4021 ggagttgtct tccaagatta taaattacta ccaacaaaaa cagtttatga gaatgttgcg
     4081 tttgcgatgc aagttattgg agctaagcgc cgtcatatta aaaaacgcgt gccagaagtt
     4141 ctagaattag taggtttaaa acacaagatg cgttcttttc caacacagtt atctggtgga
     4201 gaacaacaac gtgtggcaat tgcacgtgcg attgttaata atccaaaact attaattgca
     4261 gatgaaccta ctggtaattt ggatccagaa attgcttggg aaatcatgca tctattggaa
     4321 cgtattaacc ttcaaggaac gactgttttg atggcaacac acaatagtca aattgttaac
     4381 acacttcgtc atcgtgtcat tgagattgaa gcaggtagcg ttatccgaga tgaagagaag
     4441 ggagaatatg gttatcatga ttaatttctt tagacatttc tgggaatcgc ttaaaaactt
     4501 aaaacgtaac ttttggatga cttttgcttc tgtaacatca gttaccataa cattacttct
     4561 tgtaggcttg ttttcatcag ttttactgaa tgttgaaaag ttaacaactg atgtttcagg
     4621 taattttact atcagtgcct ttttaaatgt tgactcaacg gatgctcaaa aacaagtcaa
     4681 agataaggac ggaaagctaa aagataatcc tgattatcat aaagtttatg acaagattaa
     4741 acgtatttct ggtgtagaga aagtcactta ttcaagtaaa gctgaacaat taaaagaagt
     4801 tcaaaaagag tatggttcag atgttattga tgatacctac aaagatgctt tgttagatgt
     4861 ttacgtagtt gggacaagtt ctgcaaaagt ttctaagaat gtcagtgaag caattggacg
     4921 cattgaaggt gttgattata ctaaagaacc tattgattca acaaaactat ctaatctcac
     4981 agataacatc cgtatatggg gatttggtgg tgttgcctta ttaattgtcc ttgctatctt
     5041 tttgatttca aatactattc gtatgtcgat tatgtcacgc cgtacagata ttgaaatcat
     5101 gagattagtt ggcgctaaaa attcttatat tcgaggaccg ttcttttttg aaggtgcttg
     5161 ggtaggaatt ttaggggcta tcgttccatc gctgattttc tattttggtt atcaatttgt
     5221 gtttaacaag tttaatccaa aatttgagac aagccatgtt tcattgtatc ctatggatat
     5281 tatggttcca gcaattattg gtggtatggt gattattggt attattattg gctcacttgg
     5341 gtctgtccta tcaatgaga
//
LOCUS       DATRTN010000103         5257 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.103, whole genome shotgun sequence.
ACCESSION   DATRTN010000103 DATRTN010000000
VERSION     DATRTN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.103"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            131..1375
                     /locus_tag="VBE22_001783"
     CDS             131..1375
                     /locus_tag="VBE22_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO7203215.1"
                     /translation="MSKRQNLGISKKGAIISGLSVALIVVIGGFLWVQSQPNKSAVKT
                     NYKVFNVREGSVSSSTLLTGKAKANQEQYVYFDANKGNRATVTVKVGDKITAGQQLVQ
                     YDTTTAQAAYDTANRQLNKVARQINNLKTTGSLPAMESSDQSSSSSQGQGTQSTSGAT
                     NRLQQNYQSQANASYNQQLQDLNDAYADAQAEVNKAQKALNDTVITSDVSGTVVEVNS
                     DIDPASKTSQVLVHVATEGKLQVQGTMSEYDLANVKKDQAVKIKSKVYPDKEWEGKIS
                     YISNYPEAEANNNDSNNGSSAVNYKYKVDITSPLDALKQGFTVSVEVVNGDKHLIVPT
                     SSVINKDNKHFVWVYNDSNRKISKVEVKIGKADAKTQEILSGLKAGQIVVTNPSKTFK
                     DGQKIDNIESIDLNSNKKSEVK"
     gene            1378..2088
                     /locus_tag="VBE22_001784"
     CDS             1378..2088
                     /locus_tag="VBE22_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7203216.1"
                     /translation="MTTNRKELIRLHQIVKSYQNGDQKLQVLKNIDLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDSPTSGDYSLNGKRVEELSQTKLAQVRNKEIGFVFQQFFLL
                     SKLTALQNVELPLIYAGVPPKKRKNLAKQFLDKVELRERMNHLPTELSGGQKQRVAIA
                     RALVNSPSIILADEPTGALDTKTGEQIMQFLTELNQEGKTIIMVTHEPEIADYATRKI
                     VIRDGEITADTTDSIRID"
     gene            2103..3350
                     /locus_tag="VBE22_001785"
     CDS             2103..3350
                     /locus_tag="VBE22_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7203217.1"
                     /translation="MENWKFALSSILGHKMRAFLTMLGIIIGVASVVLIMALGKGMKD
                     SVTNEITKSQKNLQIYYKTKEDQKNEDNFGAQGAFMQGSDTNRKEPIIQESWLKKIAK
                     EVDGVSGYYVTNQTNAPVAYLEKKAKTVNITGINRTYLGIKKFKIKSGRQFQEEDYNQ
                     FSRVILLEEKLAQRLFQTNEAALNKVVTVKNKSYLVVGVYSDPEAGSGLYGSNSDGNA
                     ILTNTQLASEFGAKEAENIYFHLNDVSQSNRIGKEIGKRLTDISHAKDGYYDNFDMTS
                     IVKSINTQVGIMTGVIGAIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRRK
                     ILAQFLIESMVLTILGGLIGLLLAYGGTMLIANAQDKITPSVSLNVAIGSLIFSAFIG
                     IIFGLLPANKASKLNPIDALRYE"
     gene            3479..3751
                     /gene="rpsP"
                     /locus_tag="VBE22_001786"
     CDS             3479..3751
                     /gene="rpsP"
                     /locus_tag="VBE22_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEO7203218.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQVTIKEERVLEWLSKGAQPSDTVRNLLSKAGVMTKFHDQKFSK"
     gene            3761..4003
                     /locus_tag="VBE22_001787"
     CDS             3761..4003
                     /locus_tag="VBE22_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEO7203219.1"
                     /translation="MDTIENLIIAIVKPLISQPDQLTIKIQDGPEFLEYHLDLDTQDI
                     GRVIGKKGRTITAIRSIVYSVPTQGKKVRLIIDEKE"
     gene            4135..>5257
                     /locus_tag="VBE22_001788"
     CDS             4135..>5257
                     /locus_tag="VBE22_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7203220.1"
                     /translation="MLFDFLEEKIKLKAYLMTALLDDGEINLDHFKTEYALSEKILQG
                     LISELQILYSDFYLVVKSRTMVLFHYETLSKLETLHTIYRESNMLQFLAYFIAPQNVS
                     FSEFTAKKFISVASAYRIKKNCKLYLESVKLSIQKNKVTGPEYQIRLLIALLQYHFGF
                     IIYPFDATSEVMIRDFIEYSNFKGCDYDLKDLSEEYYYFYILVNLAWKRKEYKVEIPQ
                     TESFNQLKKSILYMELSKAAQKSIAIHLGIELNEGLLDYLYLVFCISDGPLFSNRDDI
                     NVNEEFIFASYKISKSKAFKNFCGRLLGREFIDSQHFKDINAYFLRHFICYCYYFIPD
                     FYFLNTSRLSYSKDLYHLLDRGLADIFNLKGGNLTFSKHE"
BASE COUNT         1853 a          785 c          995 g         1624 t
ORIGIN      
        1 tgtaagttct ttctatgagc aaaatagtat acttaatgag taaaaatatt atttttaaag
       61 aattttcttg acaatattat ttaaaaataa tacacttgtt ctatattaac aatacaaaca
      121 ggagatggac atgagtaaac gacaaaattt aggaattagt aaaaaaggag caattatatc
      181 agggctctca gtggcactaa ttgtagtaat aggtggcttt ttatgggtac aatctcaacc
      241 taataagagt gcagtaaaaa ctaactacaa agtttttaat gttagagaag gaagtgtttc
      301 gtcctcaact cttttgacag gaaaagctaa ggctaatcaa gaacagtatg tgtattttga
      361 tgctaataaa ggtaatcgag caactgtcac agttaaagtg ggtgataaaa tcacagctgg
      421 tcagcagtta gttcaatatg atacaacaac tgcacaagca gcctacgaca ctgctaatcg
      481 tcaattaaat aaagtagcgc gtcagattaa taatctaaag acaacaggaa gtcttccagc
      541 tatggaatca agtgatcaat cttcttcatc atcacaagga caagggactc aatcgactag
      601 tggtgcgacg aatcgtctac agcaaaatta tcaaagtcaa gctaatgctt catacaacca
      661 acaacttcaa gatttgaatg atgcttatgc agatgcacag gcagaagtaa ataaagcaca
      721 aaaagcattg aatgatactg ttattacaag tgacgtatca gggacagttg ttgaagttaa
      781 tagtgatatt gatccagctt caaaaactag tcaagtactt gtccatgtag caactgaagg
      841 taaactccaa gtacaaggaa cgatgagtga gtatgatttg gctaatgtta aaaaagacca
      901 ggctgttaaa ataaaatcta aggtctatcc tgacaaggaa tgggaaggta aaatttcata
      961 tatctcaaat tatccagaag cagaagcaaa caacaatgac tctaataacg gctctagtgc
     1021 tgtaaattat aaatataaag tagatattac tagccctctc gatgcattaa aacaaggttt
     1081 taccgtatca gttgaagtag ttaatggaga taagcacctt attgtcccta caagttctgt
     1141 gataaacaaa gataataaac actttgtttg ggtatacaat gattctaatc gtaaaatttc
     1201 caaagttgaa gtcaaaattg gtaaagctga tgctaagaca caagaaattt tatcaggttt
     1261 gaaagcagga caaatcgtgg ttactaatcc aagtaaaacc ttcaaggatg ggcaaaaaat
     1321 tgataatatt gaatcaatcg atcttaactc taataagaaa tcagaggtga aataatcgtg
     1381 acaactaatc gaaaagaact gattagattg catcagattg ttaaaagtta tcaaaatggg
     1441 gatcaaaaac ttcaagtctt aaagaacatt gatttgacgg tttatgaagg tgaatttctt
     1501 gcaatcatgg ggccatctgg ttcagggaaa tccacactaa tgaatattat tggcttgttg
     1561 gattctccaa cttcaggaga ttatagttta aatggtaaac gtgtcgaaga actaagtcaa
     1621 acaaaattag ctcaagttcg aaataaggaa atcggttttg tctttcaaca atttttcttg
     1681 ttatctaaac taacggcttt acaaaatgtt gaattaccct taatttatgc gggtgtacct
     1741 cctaaaaagc gtaaaaattt agcaaaacaa tttttagata aagttgaatt aagagagcgt
     1801 atgaaccatc tgccaacaga attatctggg ggacagaagc aacgtgtagc aatcgcgcgt
     1861 gctttggtga atagcccatc tattattcta gcagatgagc cgactggtgc cttggataca
     1921 aagacgggtg agcaaattat gcagttccta acggaattaa atcaagaagg taaaaccatt
     1981 atcatggtaa ctcatgaacc agaaatcgcc gattatgcga ctcgtaaaat tgttattcgt
     2041 gatggcgaaa ttacagcaga tactactgat agtattagaa ttgattaagg ggagattcga
     2101 ttatggaaaa ttggaagttt gccctatcat cgattctagg gcacaagatg cgtgcttttc
     2161 tgaccatgtt agggatcatc attggggttg catcagttgt gttaattatg gccttaggta
     2221 agggcatgaa agattcggtt acaaatgaga ttacaaagtc tcagaagaac cttcagattt
     2281 attataaaac aaaagaagac caaaaaaatg aggataactt tggagctcaa ggggctttca
     2341 tgcaaggtag tgataccaac cgtaaggaac caattattca agaatcatgg ttaaaaaaga
     2401 ttgctaagga agtagatggt gttagtggct actatgtaac caatcaaaca aatgctccag
     2461 tagcttacct tgaaaaaaag gcaaagacgg ttaatataac aggaattaac cgcacttacc
     2521 ttggtatcaa aaagttcaaa attaaaagtg gtcgtcaatt ccaagaggag gattataacc
     2581 aattctcgag agttatttta ctagaagaaa aattagctca gagattattc cagacaaatg
     2641 aagcagcgtt aaataaggtg gtgactgtaa aaaataaatc ttacttagta gtgggggttt
     2701 attcggatcc agaagctggt tcaggcttat atggaagtaa ttctgatggg aatgctattt
     2761 taaccaatac acagttagct tcggagtttg gtgctaaaga agcagaaaac atatattttc
     2821 atcttaatga tgtatcgcaa tccaatcgca ttggtaaaga aataggcaaa cgtttaacag
     2881 acattagtca tgctaaggat gggtactatg ataattttga catgacatct atagttaaat
     2941 ctatcaatac ccaagttggt attatgacag gtgttattgg agcgattgca gcaatttctc
     3001 tcctagttgg tggtatcgga gtgatgaata ttatgttagt ctctgtaact gagcgcacga
     3061 gagaaattgg ccttcgtaaa gcccttgggg caactcgacg taaaatttta gctcaatttt
     3121 taattgaatc aatggtctta acgattttgg gtggattaat tgggttatta ctagcctatg
     3181 gaggtacgat gttaattgct aatgcgcaag ataagattac tccttctgtg tcacttaacg
     3241 tggctatagg cagtttaatt ttctcagcat ttattggaat tatatttggt ttattacctg
     3301 ctaataaagc tagcaaatta aacccaattg atgctttacg atatgaataa aaaaacatat
     3361 gctctgtcaa gattttttct tgacagagtt tgtatttatg ttatacttag taaggtaaag
     3421 ttgttagtta agctatcaac gaccaaatat aaagaaaaag aaaagagact atttaaaaat
     3481 ggcagtaaaa atccgtttaa ctcgtatggg ttctaagaag aaacctttct accgtatcaa
     3541 tgttgcagat tcacgtgcac cacgtgacgg acgttttatt gaaactgttg gaacatacaa
     3601 cccattagtt gcagaaaacc aagttacaat caaagaagaa cgtgttcttg aatggttatc
     3661 aaaaggtgca caaccttcag atacagttcg taaccttctt tcaaaagctg gagttatgac
     3721 taaattccac gatcaaaaat tctcaaaata ataaaagcct atggatacca ttgaaaatct
     3781 cattattgct attgtgaaac ctttgatttc acaaccagat cagttaacca taaaaattca
     3841 ggatggccca gaatttttag agtaccatct tgatttagat acccaagaca tcggtcgtgt
     3901 tatcggcaaa aagggtcgta caattacagc gataaggtcg attgtctatt cggtaccaac
     3961 acaagggaaa aaagtaagac tcatcattga tgagaaagaa taatttattc taaaagatag
     4021 gaacttcctg tcttttttgt tatatctaca caaaaattgt gagtttttta gtcttttaga
     4081 atataatgga tgtgaaaggg aattttctga ttttttatta aggaggataa gatattgcta
     4141 tttgattttc tcgaagaaaa aataaaactg aaagcatacc tgatgaccgc cttacttgat
     4201 gatggagaaa ttaaccttga tcactttaaa acagaatatg ctttgagtga aaagatttta
     4261 caaggtctta tttcggaact acaaatactt tattctgatt tctaccttgt tgtcaaaagt
     4321 agaacaatgg tattgttcca ttacgaaaca ctttctaagc tagagacttt gcataccatt
     4381 tatcgagagt ctaatatgtt acagtttttg gcatatttta ttgctcctca aaatgtcagt
     4441 ttttctgaat tcacggctaa gaaattcata tccgttgcaa gtgcctaccg aataaagaaa
     4501 aactgtaaac tttatttgga gagtgttaag ttatcaatac aaaaaaataa agtgacaggt
     4561 ccagaatatc agattaggct gctgattgct ttgttacaat atcattttgg ttttattatt
     4621 tatccttttg atgctacaag tgaggtaatg ataagggatt ttattgaata ctcgaatttt
     4681 aaagggtgtg actacgacct taaagactta tctgaagaat attactattt ttatattttg
     4741 gtgaatttag cttggaaaag aaaagaatat aaagtagaaa ttcctcaaac agaatccttt
     4801 aatcaattaa aaaaatcgat tctctacatg gagttatcca aagcagctca aaaaagtatt
     4861 gctatacatc ttggtataga gttgaatgaa ggtttacttg attatctata tttagtgttt
     4921 tgtattagtg atggccctct atttagcaat cgagatgata tcaatgtcaa cgaagaattt
     4981 atttttgctt cttataagat atcaaagtca aaagccttta agaatttttg tggtcgatta
     5041 ttgggtagag aatttattga ctcgcagcat tttaaagata ttaatgccta ttttttgaga
     5101 cattttattt gttattgtta ttattttatt cctgattttt attttttaaa tacatcaagg
     5161 ctgagctatt ctaaggattt atatcaccta ttagatagag gtctggctga tatttttaac
     5221 ctgaagggag ggaatttaac tttttcaaaa catgaga
//
LOCUS       DATRTN010000104         5059 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.104, whole genome shotgun sequence.
ACCESSION   DATRTN010000104 DATRTN010000000
VERSION     DATRTN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.104"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(215..1123)
                     /locus_tag="VBE22_001789"
     CDS             complement(215..1123)
                     /locus_tag="VBE22_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7203221.1"
                     /translation="MKTIKDWQINICLATYNGQKYLRQQLDSIIQQGYTDWICLIRDD
                     GSTDDTVAIIKEYVNRDSRFIFINSNDDRKLGSHRSFYELVNYKKADFYVFSDQDDVW
                     KENRLERYLEEAEKFNQELPLLVYSNWTSVDEKLTVLKEHNPATVIQEQIAFNQINGM
                     VIMMNHELAKLWEYRQIGAHDSYVGTLAYAVGNVAYISDSTVLWRRQVGAESLNNYGR
                     QYGVATFWQMINTSFDRASLIFAQVSDKMSLERKLFFSRFIELKNANLIRRIYLLSKL
                     KLRRKSLKETVAMTILLLTGYGKPKA"
     gene            complement(1333..5034)
                     /locus_tag="VBE22_001790"
     CDS             complement(1333..5034)
                     /locus_tag="VBE22_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO7203222.1"
                     /translation="MDKHHSRKAILKLTLITTSILLMHSNQVNAEEQELKNQEQSPVI
                     ANVAQQPSPSVTTNIVEKTSVTAASASNTAKEMGDTSVKNDKTEDELLEELSKNLDTS
                     NWGADLEEEYPSKPETTNNKENNVVTNASTAIAQKVPSAYEEVKPESKSSLAVLDTSK
                     ITKLQATTQRGKGNVVAIIDTGFDINHDIFRLDSPKDDKHSFKTKAEFEELKAKHNIT
                     YGKWVNDKIVFAHNYANNTETVADIAAAMKDGYGSEAKNISHGTHVAGIFVGNSKRPA
                     INGLLLEGAAPNAQVLLMRIPDKIDSDKFGEAYAKAITDAVNLGAKTINMSLGKTADS
                     LIALNDKVKLALKLASEKGVAVVVAAGNEGAFGMDYSKPLSTNPDYGTVNSPAISEDT
                     LSVASYESHKTISEVVETTIEGKLVKLPIVTSKPFDKGKAYDVVYANYGAKKDFEGKD
                     FKGKIALIERGGGLDFMTKITHATNAGVIGIVIFNDQEKRGNFLIPYRELPVGVISKV
                     DGERIKNTSSQLTFNQSFEVVDSQGGNRILEQSSWGVTAEGAIKPDVTASGFEIYSLT
                     YNNQYQTMSGTSMASPHVAGLMTMLQSHLAEKYKGMNLDSKKLLELSKNILMSSATAL
                     YSEEDKAFYSPRQQGAGVVDAEKAIQAQYYVTGNDGKAKINLKRVGDKFDITVTIHKL
                     VEGVKELYYQANVATEQVNKGKFALKPQALLDTNWQKVILRDKETQVRFTIDSSQFSQ
                     KLKEQMANGYFLEGFVRFKEAKDSNQELMSIPFVGFNGDFANLQALETPIYKTLSKGN
                     FYYKPNDTTHKDQLEYNESAPFESNNYTALLTQSASWGYVDYVKNGGELELAPESPKR
                     IILGTFENKVEDKTIHLLERDAANNPYFAISPNKDGNRDEITPQATFLRNVKDISAQV
                     LDQNGNVIWQSKVLPSYRKNFHNNPKQSDGHYRMDALQWSGLDKDGKVVADGFYTYRL
                     RYTPVAEGANSQESDFKVQVSTKSPNLPSRAQFDETNRTLSLAMPKESSYVPTYRLQL
                     VLSHVVKDEEYGDETSYHYFHIDQEGKVTLPKTVKIGESEVAVDPKTLTLVVEDKAGN
                     FATVKLSDLLNKAVVSEKENAIVISNNFKYFDNLKKEPMFISKEGKVVNKNLEEIALV
                     KPQTTVTTQSLSKEITQSGNEKVLTSTNNNSSRVAKIISPKHNGDSVNHTLPSTSDRA
                     TNGLFVGTLALLSSLLLYLKPKKTKNNSK"
BASE COUNT         1507 a          980 c          761 g         1811 t
ORIGIN      
        1 aggctttacc ttcatacttt tcctgtgtaa aacaaaaaag gagtagtgac tgctactcct
       61 tttccaaaac ccgaaattaa tgcgagccaa ggttctacct tgttctttaa tattattata
      121 cactaatttc gttaagtgtc aacaactttt tgaaaaataa atattttcta tatttttaac
      181 tacaactcaa cttgatttcc ttcaaccaaa aaaattatgc ttttggttta ccgtaacctg
      241 ttaaaagtag gatagtcata gcaactgttt ctttcaatga tttccgacga agttttaatt
      301 ttgataaaag ataaatcctt cttattagat tggcattttt taattctata aaacgactaa
      361 agaagagttt acgctccagg gacattttat cggatacttg agcaaaaata agcgaagcac
      421 gatcaaaaga agtatttatc atctgccaaa acgtagccac cccatattgt ctcccgtaat
      481 tgtttagcga ctctgcccct acttgtctac gccaaaggac tgtactgtca gaaatataag
      541 ccacatttcc aacggcgtaa gccagagttc caacataaga atcatgggcg ccaatttgac
      601 gatattccca aagctttgct aactcatgat tcatcatgat aaccatacca tttatttggt
      661 taaaggcaat ttgttcttga ataactgttg ctggattatg ttctttcaat actgttaatt
      721 tttcatctac agaagtccag ttcgaataaa caagcaatgg taattcttgg ttaaatttct
      781 ctgcttcttc aaggtaacgt tctaaacgat tttctttcca aacatcatcc tggtctgaga
      841 aaacataaaa atctgctttt ttatagttaa ccaactcgta aaaactgcgg tgacttccta
      901 actttctgtc atcgttagaa ttaataaata taaaacgtga atctctatta acatattctt
      961 taataatagc tactgtatca tcagtagatc catcgtccct tattaaacaa atccagtcag
     1021 tataaccttg ttgtattatg ctatctaatt gctggcgaag gtatttttga ccattatagg
     1081 tcgctaagca aatatttatt tgccaatctt ttatcgtttt cattgctatc aaaactcctt
     1141 aaggctagtt catcttatga aagcttacaa agtgaacggt atcacatact aatcgtaatt
     1201 ataatattat aatattatat gtcattaatc aatataaaat taatcaaaaa taagagtcaa
     1261 taccctaaaa gtccagctta acataatgtt aaactgaact tttcatcata ttgcagagta
     1321 gtttacttag ttttatttac tattattttt agtctttttg ggtttcaagt aaagaagtaa
     1381 actagataac aatgccaaag taccaacaaa tagaccattc gttgctctat ctgatgtact
     1441 aggtaaggta tggttaacag aatccccgtt atgtttaggt gatatgatct tagctactct
     1501 gctactatta ttgtttgtag aagtgaggac tttctcattt cctgattgag ttatttcttt
     1561 agacaatgat tgagtagtaa ctgtagtttg cggcttaact aatgctattt cttctagatt
     1621 cttgtttact acttttcctt ctttagaaat aaacataggt tcttttttca agttatcaaa
     1681 atatttgaaa ttgttagaaa ttactatagc gttttctttc tctgatacta ctgccttatt
     1741 caagaggtca gacaatttta ccgttgcgaa attaccagct ttatcttcca caacaagtgt
     1801 caaggtctta gggtctactg caacctcact ctctcctatc ttaactgttt taggaagtgt
     1861 cactttacct tcttgatcta tatggaaata atggtaagaa gtctcatctc catattcttc
     1921 atcttttaca acatgagata aaactaattg tagacgatat gtaggaacat aactactttc
     1981 cttaggcatg gctaagctta atgttcgatt agtttcatca aactgagctc gtgaaggaag
     2041 atttggtgac ttagtactta cttgaacttt aaagtctgac tcctgactat ttgctccttc
     2101 tgctactggt gtgtaacgta aacgataagt ataaaaacca tctgctacaa ctttgccatc
     2161 cttatctaaa ccactccact gaagggcatc catacgataa tgaccatcac tttgctttgg
     2221 attattatgg aaatttttac gataagatgg taaaacctta ctttgccaaa taacatttcc
     2281 attttgatct agaacttgag cagaaatatc cttaacattt cttaagaaag ttgcctgggg
     2341 agtgatttca tccctatttc catctttatt tggagaaatg gcaaaatatg gattattcgc
     2401 tgcatctctt tccaaaagat gaattgtttt atcctcaacc ttattctcaa aagttcctaa
     2461 aataattctt tttggactct ccggtgctaa ttctaactcc ccaccatttt tgacataatc
     2521 aacatagccc caagacgctg attgtgttaa caaggcagta tagttgttgc tttcaaaagg
     2581 agctgattca ttgtactcca attggtcttt atgagttgta tcatttggtt tatagtagaa
     2641 attaccttta gaaagcgtct tataaatcgg tgtttcaagt gcttgtaagt tcgcaaaatc
     2701 accattaaat cctacaaaag gaatactcat taactcctga ttactatcct tggcttcttt
     2761 aaaacgtaca aaaccttcta agaaataacc atttgccatc tgttctttta atttctgact
     2821 aaattgacta gaatcaatag taaatcgaac ttgtgtttct ttatcacgaa gaattacttt
     2881 ctgccaatta gtatctagca aggcttgtgg tttaagggca aatttacctt tatttacttg
     2941 ttctgttgct acattagctt gataatacaa ttctttgaca ccttctacaa gtttatgaat
     3001 tgtaactgtg atatcaaatt tatctcccac tcgtttgaga ttaattttag ctttgccatc
     3061 gtttccagta acataatatt gagcttggat agctttttca gcatcaacta cacctgcacc
     3121 ttgctgacgt ggtgaataaa acgccttatc ctcttcacta tataatgctg ttgctgagct
     3181 catgaggatg tttttagaca attctagcaa ttttttagaa tctaaattca tccctttata
     3241 tttctcagcc aaatgacttt gaagcattgt cattaatcct gcaacatgtg gtgaagccat
     3301 acttgtacca gacattgttt ggtattgatt attataggtt aaagaataaa tttcaaagcc
     3361 agaagctgtt acatcaggct tgattgctcc ttcagctgtc acgccccaac ttgattgttc
     3421 cagtatacga ttgccacctt ggctatcaac tacttcaaaa ctctggttaa atgttaactg
     3481 acttgaagta ttttttatac gctcgccatc tactttacta ataaccccca caggtaattc
     3541 acggtaagga attagaaaat ttccacgttt ttcttgatcg ttaaaaataa cgataccaat
     3601 aacacctgca tttgtagcat gagtgatttt agtcataaaa tcaagtccac caccacgctc
     3661 aattaatgca atcttacctt taaagtcctt accttcaaag tctttttttg caccataatt
     3721 ggcataaacc acatcgtagg ccttaccttt gtcaaaaggt ttagaagtca caatcggcaa
     3781 cttaactaac ttaccttcaa tagttgtttc aacgacctca ctgatagttt tatgtgattc
     3841 atagctagca acactcaaag tatcttcaga aatagctgga ctattaaccg taccgtagtc
     3901 aggattagtt gataatggtt tgctataatc cataccaaat gcaccttcat ttccggcagc
     3961 cacaacaact gcaacgccct tctcagaagc taatttaagt gctaatttaa ctttatcatt
     4021 gagagcaatt aaagaatctg ctgtttttcc aaggctcata ttaatcgttt ttgctcctag
     4081 attaacagcg tctgtgattg ctttagcata tgcttctcca aatttgtccg aatcaatttt
     4141 atctggaata cgcattaata agacttgagc atttggcgct gcaccttcta aaagaagacc
     4201 attgattgct ggacgtttac tattacctac aaaaatacca gcaacgtgtg taccatgcga
     4261 aatattcttt gcttctgaac cataaccatc tttcatagct gctgcaatat cagccaccgt
     4321 ttctgtattg ttggcgtagt tatgtgcaaa aacaatctta tcgttaaccc atttcccata
     4381 agtgatatta tgttttgctt ttaattcctc aaattctgcc ttagttttaa agctgtgctt
     4441 atcatctttt gggctatcta aacgaaaaat atcatggtta atatcaaagc cagtatcaat
     4501 aatagctact acatttccct ttcctctttg ggttgtggct tgtaattttg ttattttaga
     4561 tgtatcaaga acagcaagcg atgacttgct ttctggcttc acctcttcat acgctgaggg
     4621 aactttctgt gctattgcag ttgaagcatt tgttactaca ttgttttctt tattgttggt
     4681 tgtctctggt ttagagggat attcttcttc aagatcagcc ccccaattag acgtatcaag
     4741 gtttttagat aactcttcta ataattcatc ttctgtttta tcatttttta cagatgtatc
     4801 acccatttct ttcgctgtat tactagcaga agcagctgtt acagatgttt tttcaacaat
     4861 attagtagtt actgatggcg atggctgttg agcaacatta gcaattacag gtgattgctc
     4921 ttggtttttt aattcttgct cctctgcatt cacttgattg ctatgcatta ataaaatact
     4981 agttgttata agtgttaact ttaaaatagc ctttcttgag tgatgtttat ccacttttcc
     5041 tatttcctcc aaaaatcag
//
LOCUS       DATRTN010000105         5035 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.105, whole genome shotgun sequence.
ACCESSION   DATRTN010000105 DATRTN010000000
VERSION     DATRTN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.105"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            154..468
                     /locus_tag="VBE22_001791"
     CDS             154..468
                     /locus_tag="VBE22_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO7203223.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRVNGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVRLINPRITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            489..866
                     /locus_tag="VBE22_001792"
     CDS             489..866
                     /locus_tag="VBE22_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEO7203224.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGSVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            876..1649
                     /locus_tag="VBE22_001793"
     CDS             876..1649
                     /locus_tag="VBE22_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203225.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKDLRFEKRIAYRKYEAISQPTFDKQARIYNTQLF"
     gene            1671..3074
                     /locus_tag="VBE22_001794"
     CDS             1671..3074
                     /locus_tag="VBE22_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO7203226.1"
                     /translation="MPLLHDRGKRIKANDKDLVYHFVLISLLIVFLVVLLPFHWRTLQ
                     SVNWQTVQLDTFFYQLHTPYFWSSILTAFFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLSSKKTKQRIRHFPKMYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVIDN
                     VYYRKDDMLACISQFYDDMIARSETMKQHPNYKTGENYAYLGLPANFLIFDEYVAMMD
                     MLGRDSAQVMNKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3256..4440
                     /locus_tag="VBE22_001795"
     CDS             3256..4440
                     /locus_tag="VBE22_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEO7203227.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVSQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYGFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPELAKKCKQEECISVFRSFKNYRSGELVHRDEKPDM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDIALEDAEIKNRFEIRLKNDRATQAMKDLL
                     EHQNAEKTAFEIINRYIRFADKDETKRRSDWQTNERWEWFIGKNRGALRLTIQPEPYS
                     FERTLNWLHHQVAPTLKVASILDILNGTTIISTMIHEAKLTEKHEKLIEQQHLGMEDL
                     IT"
     gene            4437..4709
                     /locus_tag="VBE22_001796"
     CDS             4437..4709
                     /locus_tag="VBE22_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203228.1"
                     /translation="MSLLTEELKKLGFQAYIQNTGKYTSLIIEGKRQAGDTIYTYDFY
                     KVSFYKNYTSRITVYGEHLTPFQLLKRVKSYIYYREKYLKERRTIT"
     gene            4706..4927
                     /locus_tag="VBE22_001797"
     CDS             4706..4927
                     /locus_tag="VBE22_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203229.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
BASE COUNT         1678 a          861 c         1019 g         1477 t
ORIGIN      
        1 cgaaatggaa ttctaaactc aatattcatt taaatgatag acgttcataa tgagcgtcta
       61 ttttttatgc tttgaaaggt tgtgatacta acaaattttt aaatgaataa agtaagccaa
      121 aaatatttta tgtgagaaag gaacgataca cttatggaat taaaatatgt cattcccaat
      181 atggaaaaaa catttggtca attagaatat gctggtgaag ggaatattga acaacgtcga
      241 gtcaatggca gaccaactat tctctctcgt agctacaatt tatactcaac gattcaacga
      301 gcagatgata ttgttgtcat tcttccctcc gaagctggcg agaaacattt tgaatccgac
      361 gaaaaagtca gactcatcaa cccacgtatt accgcagaag gttataaaat tggcaataga
      421 ggatttacca attacattat gcacgcagac gatatggtga aagcttaaga aaggaaaggt
      481 gaaaaaatat gagattagca gaaggtattg tgattgaaaa agaccagaca tttggcacat
      541 tgaaattttc cgctttacgt cgtgaggttc gtattcaaaa tgaagatggt tcggtttccg
      601 aagaaattaa agaacgtacc tatgacttaa aatcaaaagg acaaggacgc atgatacaag
      661 tcagtatccc cgcttctgtt cctttgaaag aatttgacta caacgcagaa gtggaattaa
      721 tcaatcctgt ggcagatacc gtagcgacgg ctacttttcg aggggcagac gttgattggt
      781 acgtgaaagc agacgatctt gtattaaaag gacagacaaa acctcatcag aaatataaaa
      841 aagaagcgtc cgaaagtgaa aaataggtgt tgcttatgac aaacacacca ttgattttga
      901 aagaaattcc gaaagatgaa gccatttctt ttattcggca ataccattat tcaaagatac
      961 tccctagatt gtgtaaatac tttctaggga ttttttcaga agaaaaatta ttaggcgttg
     1021 tggaattagg ctggggtacg caacccctac aaaccatacg caaactcttt cccgacagtt
     1081 cccttcaaac cacagattat ttagaaattg gaaagatgtg ttttcttcca gaaatgaatc
     1141 aaaccaatta ttttggcagt caagctcttt cagctctaat taagtggttg aaagaacata
     1201 cggactgtca ttttttatat acattggcag atggaattga gggaaaatgt ggctatgtct
     1261 atcaagcgtc caacttcttt tactgcgggt atttcaagac aagtgtctat cgtgataaac
     1321 agtcatggga aaagatacac cctcgaagtg ctcgcctttt attagaggaa aatgctcgtt
     1381 ttgaacaggt agagaaaaag cattggcttt cccaggcatt ttgcgaatac aaagggattg
     1441 agaaaatcaa tggacgcatg tttcgctatc tctatccgtt gacaaaagaa gcgaaaaaac
     1501 tgttaggaca cacgctctat cgacgtcatt attatccgaa agaaaaagat ttacgctttg
     1561 aaaagcgaat cgcttatcgg aaatatgaag ccatttctca accaactttt gataaacagg
     1621 ctcgtatcta caatacacaa ttattttgat agatggaaag gaggattttt atgccattgc
     1681 ttcatgacag aggaaagcga atcaaagcca acgataagga cttggtctat cattttgtgc
     1741 tgatttcatt gctgatagtt tttctagtcg ttctactccc ttttcattgg agaacgttac
     1801 aatcggtgaa ttggcaaaca gttcagttag atacattttt ctatcaactg catacgccat
     1861 acttttggag cagtattctc actgcttttt ttgtatgcgg actcatcagc tggctctatt
     1921 atcactatcg catagattct ataaaacagt tagagcaccg tcaaaagtta gctcgtatga
     1981 ttttagaaaa ccattggtat gaagccgaag aaactcaatc agagggattc tttaaagact
     2041 tatccagcaa gaaaaccaaa caacgaatcc gccattttcc taaaatgtat taccgaatga
     2101 aagatggact gatccatatt catgtagaaa tcactttagg gaaatatcaa gatcagctct
     2161 tgcacttaga aaacaaacta gaaacaggct tgtattgtga attagtggaa aaaacgttga
     2221 aagattctta tgtagagtat gttctacttt atgacaccat tggcaatcga attggcattg
     2281 aagatgtggt agctcaagat ggacgtgtca aattgatgga atcggtctat tgggagtttg
     2341 attctctccc tcacatgtta attgctgggg gcacgggtag tggaaaatcc tattttattc
     2401 tcacattgat tgaatctctc ctgcatacaa atgcgaagct ttatattctc gaccctaaaa
     2461 atgctgattt ggcagactta ggagcggtca tagataatgt ctattaccgt aaagacgata
     2521 tgctggcatg tattagtcaa ttttacgacg atatgattgc ccgtagtgaa accatgaagc
     2581 aacacccgaa ctacaaaaca ggagaaaatt atgcgtatct tggtctacct gcgaacttcc
     2641 tgatttttga tgaatatgtg gcgatgatgg atatgttagg tcgtgatagt gcacaagtta
     2701 tgaataaact caaacaaatt gttatgctcg gacgacaagc aggcttcttt ttaattcttg
     2761 cctgtcagcg tccagacgca aaatatttta gtgatggtat tcgagataac tttaacttcc
     2821 gtgtggcttt gggtcgtatg tctgaaatgg gcttcggtat gatgtttgga agcgatacac
     2881 aaaaacaatt tttcttaaaa cccatcaagg gaagaggtta tgtggataca ggaaaaagtg
     2941 tgattagtga gttttataca ccccttgtac caaaaaggta tgatttctta ggagaaatag
     3001 gaaaagtcat tcaaaaaaag caatctgagc ctgtaccgca cgaagtgaaa ggtacaggtt
     3061 cagacttgct ttgattggtg tggcgttagc cacgccaatc ctttagcccc ctctacctaa
     3121 caggggggca caaatacaac tgaaaacagg caattctcat caaccaatgt tgatggtaca
     3181 aggaattgcg gaaaatagga aagatgtaaa ctcaagggtc atagtttaca tcttttttag
     3241 attggaggtt tgcgaatgga aaacgaaaaa atttggatta aggaattgaa agaaaagcga
     3301 ctggcttatg gcgtttctca aaataaactg gcggttgctt ctcatattac cagaccttac
     3361 ctatcggata ttgaaacagg caaggcagtc cctactcaac aggtgaaaga agatttactg
     3421 aacgctttgg aacgcttcaa tcccgataac ccattggaga tgttattcga ctatgtacgg
     3481 attcgattcc ctaccaatga tgtgtctcaa attatcggtg aggttttacg cttgaatatg
     3541 gattatatgc tacacgaaga ctttggctat tattcttatc ccgaacacta tcgtttcggt
     3601 gatattgttg tcctagtctc tcatgatgta agtaaagggg ttttattaga actgaaagga
     3661 aaaggctgtc ggcaatttga aaactttcta ttggctcaac atcggagctg gtatggtttc
     3721 tttcaagatt gcatggaaca taaaggtatt ttcaaacgct tggatttagc catcaacgat
     3781 aaaacaggga ttttgaatat tccagaattg gcaaagaaat gtaaacagga agaatgtatt
     3841 tccgtctttc ggagctttaa aaattatcgc agtggtgaat tggttcaccg tgatgaaaaa
     3901 ccagacatgg gaaacaccct ttatattggc tcattaaaaa gcgaagtcta tttctgtata
     3961 tatgagaaag attatgagca atacgtgaaa aatgatattg ccttagaaga cgcagaaata
     4021 aagaatcgct ttgaaattcg tttgaaaaac gacagagcca ctcaagccat gaaagatttg
     4081 ttggaacatc aaaatgcaga aaaaacagcg tttgagatta tcaatcgtta tattcgcttt
     4141 gccgataagg acgaaaccaa gcgacggagt gattggcaaa ccaatgaacg ctgggaatgg
     4201 tttatcggca aaaatcgtgg agcattacgc ctaaccatac agccagaacc ttattctttt
     4261 gaacgaacat tgaactggtt acatcatcaa gtcgccccta cattaaaagt cgcaagcatt
     4321 ttagatattc tcaacggtac aaccattatt tccaccatga ttcatgaagc caaactgaca
     4381 gaaaaacatg agaaattaat tgaacaacaa catctaggca tggaggattt gattacatga
     4441 gcctcttaac agaagaatta aagaagttag ggtttcaagc gtatatacaa aatacaggga
     4501 aatatacgtc tctcattatt gagggaaaac ggcaagctgg tgataccatc tatacctatg
     4561 atttttacaa agtgagcttt tataagaatt ataccagtcg aataactgtt tatggagagc
     4621 atttaactcc ttttcaatta ttaaagcgtg taaaaagcta tatctactat cgagaaaaat
     4681 atcttaagga aaggaggaca atcacatgaa ttttgggcag aatttgtaca actggttctt
     4741 ggataatgca cagtcattgg tattgttagc gattgtcgtc attggactgt ttttaggttt
     4801 taaacgagaa ttttcaaaac ttattggctt tttgattatt gctttaatcg ccgtcggact
     4861 tgtgttcaat gcagctggcg tgaaagatgt gttattaaac ttatttaacc gaatcattgg
     4921 tgcttaacag atatttttca tgataaaata ggaagtatat attttgttat gaaaggatcc
     4981 atgaatatgt ttacaaatga tcaatttaaa tctcttttaa ttaaaacatt agaaa
//
LOCUS       DATRTN010000106         4785 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.106, whole genome shotgun sequence.
ACCESSION   DATRTN010000106 DATRTN010000000
VERSION     DATRTN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.106"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..186
                     /locus_tag="VBE22_001798"
     CDS             <1..186
                     /locus_tag="VBE22_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203230.1"
                     /translation="NGHILHASIREIPGLLTVNSQVSKLFQTRVNEVYLKQIDDLVMY
                     QNIYINLIKPDELDSFL"
     gene            421..735
                     /locus_tag="VBE22_001799"
     CDS             421..735
                     /locus_tag="VBE22_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203231.1"
                     /translation="MKTRNRKGGYLANTANEYIDSKQAIHCLSVELEPQIRFEEGQPT
                     GEIIAYKAWFSQKGLPPFMVKFENEVTLPAYMVMVQFENLQACEVGFNVYFKADNLKE
                     VK"
     gene            735..1238
                     /locus_tag="VBE22_001800"
     CDS             735..1238
                     /locus_tag="VBE22_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203232.1"
                     /translation="MTKKELSQYLLQSLNMGLGALMQGETSYTNSFDCKIMEEGFLFL
                     PRLPAGYIIDDELYQKIFLIANASLFPRYTLLKQNSAYFMALDTEDIHVQRGLFFPWK
                     EGVSERLIISDLEDFASSQKETLIPIMKNLSLDFNKVNHIAIAGNSGSGKSYALTYFL
                     SLLKGIS"
     gene            1302..1811
                     /locus_tag="VBE22_001801"
     CDS             1302..1811
                     /locus_tag="VBE22_001801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO7203233.1"
                     /translation="MIHPQKNRSKSDFVSEINENLSSCLELIQQRQEILYENPDHEFD
                     HLTIVIDEVLALSEGVNKVIKESFFSLLSQIALLGRATKIHLLLVSQRFDHNTIPISV
                     REQLNVLIQIGNINKKTTQFLFPDLDPEGIVIPIGKGTGLIQILDNEHPYQVLPLLCP
                     TYYTKKGIL"
     gene            2118..2426
                     /locus_tag="VBE22_001802"
     CDS             2118..2426
                     /locus_tag="VBE22_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203234.1"
                     /translation="MIETILRQEEHSEPVMTIENQTVTRFNPILRSFNRVVSKCTVDA
                     RKDSVSVFLKVPKTQQAQKLLREMEEHIKEEISSRNPKYYFSTPNREKSKLWFTGTKR
                     "
     gene            2561..3127
                     /locus_tag="VBE22_001803"
     CDS             2561..3127
                     /locus_tag="VBE22_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEO7203235.1"
                     /translation="MSKDKRSNKWAFLLYQESVPKNYLEVLEELHIPFVLSPWHDKDV
                     NKETGEFKKAHKHGALFFESLKSYSQVSELLTKHLNTPSHVEVIMSPKGMYDYFIHAE
                     NPDKTLYDINDIESGCGFELDKFLTSNNNDKFLSLVIDIIEEQNFTEFNNLVRYARSE
                     NPQLLALIMNKTYFFAKYLDSRRYSRRK"
     gene            3131..3364
                     /locus_tag="VBE22_001804"
     CDS             3131..3364
                     /locus_tag="VBE22_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEO7203236.1"
                     /translation="MTRTINHKRLMELGFPEHTARNIIKQSKAIAVAKFEETSNSKNN
                     LVKLRKSPFENSRLDLAPTYIVEDLLGFELPFK"
     gene            3382..4563
                     /locus_tag="VBE22_001805"
     CDS             3382..4563
                     /locus_tag="VBE22_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO7203237.1"
                     /translation="MANKKYRGVSRDKQGRIYYQTEFKANNITGKRQRKKSYKDQFGN
                     PFRSEKQAYDELCRVRVEFQTKDNNDGYYLSFSQYMEEVYLPYYKQTVQAVTYRTALT
                     HHKMFVEIFGSKRLSEIDVRECEKFRIYLIEKYSPNYAKGVWIRFKHCIGYAERLGYI
                     DEFPCKSLDNPKGKRPDTKFWTLEDLKKVVATFDLRDYEERHRFTAIWLYFFTGLRVS
                     EGLSLIWSDIDFDKKLLHVQSTLEYEGNGVFTRKNPTKTSAGIRWIELDDETLNVLLR
                     WRSIQVENGDDSYVLARFGKPLNKCTLSRILKRQAKKAGVEVITGKGLRHSHDSFLIN
                     VLGKDVLYVSKRSGRVDKATTLNTYSHLYDSKKASGGREISQALHKFGVSSNPTKTPL
                     N"
BASE COUNT         1654 a          824 c          884 g         1423 t
ORIGIN      
        1 aatggtcata ttttacatgc tagtataaga gaaattccag gactattaac agtcaatagt
       61 caggtatcaa aattatttca aacaagagtt aatgaagttt acttaaaaca aatagatgat
      121 ttagtcatgt atcaaaacat atatatcaac ttaatcaaac cagatgaact agattcattt
      181 ttataaccaa attattaaac ggacagttac aggagtactt gtccgttttc attttgtcca
      241 taaaagaagt agaaactgag aactaaagta agaatttaga agaatgtatt ctccgatata
      301 ctaaagacat caaagcacag caaatctttg gtagtcaacg ctcaagagag ttgcaaatct
      361 gttgagtcac ttttcaggaa gttgctgatt cctgaacggg tttttaatgg aggataaaac
      421 atgaagacaa gaaatcgtaa aggtggttat ttagcgaata ctgcaaatga gtacatcgac
      481 tctaaacagg caattcattg cttgagtgta gaacttgaac cgcaaattag gtttgaagag
      541 ggtcagccta ctggggagat tatcgcttat aaggcttggt tctctcaaaa agggcttccg
      601 ccttttatgg tgaagtttga aaatgaagtg acactaccag catatatggt aatggtgcaa
      661 tttgagaatc ttcaagcttg tgaggttggg ttcaatgttt atttcaaggc agacaatctc
      721 aaggaggtca aataatgacc aagaaggaat tgtcacagta tctgcttcaa agtctcaata
      781 tgggacttgg agctcttatg caaggggaaa caagttatac caatagtttt gactgcaaaa
      841 ttatggaaga aggatttctc tttcttcccc gcttaccagc gggatatatt attgatgatg
      901 aactctatca aaaaatattc ctaatcgcca atgcttcact cttccctcgt tacacactat
      961 tgaaacaaaa ttctgcctat ttcatggcac ttgatacaga agatattcat gttcaacgtg
     1021 ggctattttt cccatggaaa gaaggcgtat ctgaacgctt aatcatttct gacttggaag
     1081 atttcgcaag ctctcaaaag gaaactctga ttcctatcat gaaaaatcta tctcttgatt
     1141 ttaacaaggt caatcatatt gccattgcag gaaattcagg atcaggtaaa tcttatgctc
     1201 tgacctactt tctgagtctc ttaaaaggga tttcttaact gattatcgtt gaccctaaat
     1261 atgatactcc aagcagatgg gcaagagaaa atggtattgc agtgattcac cctcaaaaga
     1321 atcgctctaa gtctgatttt gtatcggaaa tcaatgagaa tctaagttcg tgcttagagc
     1381 ttatccaaca acgccaagaa attctctatg aaaatcctga ccacgaattt gaccatctaa
     1441 ccattgtcat cgatgaagtt ttggcacttt cagaaggagt gaacaaagtt atcaaagaat
     1501 cctttttctc actactttct caaatagctt tacttggtag agctactaaa attcacttac
     1561 tactggtatc acaacgattt gaccataaca cgattccgat ttccgtacgt gaacagttaa
     1621 atgtcttgat tcaaattggc aacatcaata aaaaaacgac tcagttcctc tttcctgact
     1681 tagaccctga aggtattgtc attccaatag gaaaaggaac tggcttaatt caaatacttg
     1741 ataacgaaca tccttatcaa gtattgccac tcctttgtcc aacctactat actaagaaag
     1801 gtattttata aaatgaaaag aagtttcttc caaaaaacgt ttaacctgaa tctgaatttg
     1861 cttttctttg gtttcattgc cttcttaatc agttatggat tccatgtact aggcaatcaa
     1921 atcaagacaa gttctttctt gagctctcaa tcagtctttg cagatattca aactaagtta
     1981 caagtggtag accagtatac tcagtcaata gcatatttgt ttattctgat tgccattatt
     2041 ctaaccttga ctgaactctg tcagcgaatt atgaaggact caatatggaa ctattttaag
     2101 tctgtttatc aaaccttatg attgagacaa ttcttagaca ggaagaacat tctgaacctg
     2161 ttatgacaat agaaaatcaa acggtcactc gctttaatcc tatcctaaga agtttcaatc
     2221 gggttgtttc taagtgcacg gtggatgccc gaaaggattc tgtttctgtc tttctaaaag
     2281 tccctaaaac gcaacaggca caaaaactct tgcgagaaat ggaagaacat atcaaggaag
     2341 aaatttcgag ccgaaatccc aagtattatt tctctacccc aaatcgggag aaaagtaagc
     2401 tctggtttac aggtactaaa cggtaaggga aagagtggtg cttggctcac gaagtgaaag
     2461 ccaaaagcac cactctttct tataactgtt tttaacatgt gtaatggggt tactacgccc
     2521 ccccattaca ccaataatca ataatcacga aaggaagtat atgtcaaaag acaaacgctc
     2581 gaataaatgg gcttttctgc tctatcagga aagtgtgcct aagaactatt tagaggtgct
     2641 tgaagagtta catatcccct ttgttttaag tccgtggcat gataaagacg taaacaagga
     2701 aacaggggaa ttcaaaaaag ctcacaaaca tggggctctc ttctttgaat ctctcaaaag
     2761 ctattcacaa gtctctgaat tattgacgaa gcacctaaat acaccaagtc atgttgaagt
     2821 tatcatgtca cctaaaggaa tgtatgacta ctttattcat gcggagaatc cagataaaac
     2881 actctatgat atcaatgaca ttgaatcagg ttgtggattt gagttagata aatttctcac
     2941 cagcaataac aatgataaat ttttatctct ggtaattgac attatcgaag aacaaaattt
     3001 cacagagttt aataatctgg ttagatatgc gagaagtgaa aatcctcaac tgcttgcctt
     3061 gataatgaat aaaacctatt tctttgcaaa atatctggat tcgagaagat attcaaggag
     3121 gaagtaacga ttgacaagaa caataaatca taaaagatta atggagcttg gctttccaga
     3181 acatactgca agaaatatta ttaaacaatc aaaagcgata gcagtagcaa agtttgaaga
     3241 aactagtaat tctaaaaata atttggtaaa attgaggaaa tcgccctttg aaaattctag
     3301 actagacctt gcacctacct atattgtaga agatttgcta ggcttcgagt taccttttaa
     3361 gtaaagaaag gaaacttgaa aatggcaaat aaaaaatatc gtggtgtatc acgtgataaa
     3421 caaggaagaa tatattatca aacggagttc aaggctaata atataactgg taaacgccaa
     3481 cgcaaaaaga gttataaaga ccaatttgga aatccttttc gttccgaaaa gcaagcatat
     3541 gatgaacttt gtagagtgag agttgaattc caaacgaaag ataacaatga tggttattat
     3601 ctaagttttt ctcaatacat ggaagaagtg tatctacctt attacaaaca aacggttcaa
     3661 gcggtcactt atcggactgc tctaactcat cacaaaatgt ttgttgaaat ctttggtagt
     3721 aaaagacttt ctgaaattga tgttcgagag tgcgaaaaat tcaggatata tctgattgaa
     3781 aagtattcac caaattatgc taaaggtgta tggattcgat ttaagcattg tattgggtac
     3841 gcagagaggc tgggatatat tgatgaattc ccttgcaaat ctcttgataa tccgaaaggg
     3901 aaaagaccag atacaaagtt ttggactcta gaagacttaa aaaaagtagt cgctaccttt
     3961 gacctacggg attatgaaga aaggcataga tttactgcca tatggctcta tttctttacg
     4021 ggtcttcgtg tatcagaggg tttaagttta atttggtctg atattgattt cgacaaaaag
     4081 ctcttacatg ttcaaagtac acttgagtat gaaggaaacg gagtctttac tcgcaaaaat
     4141 ccaactaaga cttcagctgg tataaggtgg attgagttag atgatgaaac tctaaatgtt
     4201 cttctaagat ggcgttcaat tcaagtggaa aatggtgatg acagttatgt attggctaga
     4261 ttcggaaaac cactcaataa atgcacatta agtagaattc taaaacgcca agctaaaaaa
     4321 gcaggagtag aagtaatcac tggaaaaggg ttgcgacatt ctcacgacag ttttttgata
     4381 aacgtactag gaaaagatgt tctgtatgtt tctaaacgtt ctggacgagt tgataaggca
     4441 actaccttga atacctattc tcatttgtat gatagcaaaa aagctagtgg tggaagagag
     4501 atatcccaag cattacataa gtttggagta tcatcaaacc ccaccaaaac cccactaaac
     4561 taaataagag caagaaaaac ccgattatat caatgtttat aagcattgtg taactgttac
     4621 tcttaaataa tcatctattg gttgtttaaa ggtgaaagcc tagttttaaa gtcttttcgt
     4681 taaagttaag gcgattaaaa aaggaaagat tcaattgaat cttccccaca aacaccccca
     4741 caatattttg tgggggtgtt ttgtttaata tggatttaga atatc
//
LOCUS       DATRTN010000107         4762 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.107, whole genome shotgun sequence.
ACCESSION   DATRTN010000107 DATRTN010000000
VERSION     DATRTN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.107"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            12..99
                     /locus_tag="VBE22_001806"
     tRNA            12..99
                     /locus_tag="VBE22_001806"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46..48,aa:Ser,seq:gct)
     gene            335..1678
                     /locus_tag="VBE22_001807"
     CDS             335..1678
                     /locus_tag="VBE22_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7203238.1"
                     /translation="MKKRILSAVLVSGVTLGTAAVTVNADDFDSKIAATDSVINTLSG
                     QQAAAQNQVTAIKGQVGALESQQSELEAQNAQLEAVSQQLGQEIQTLSNKIVARNESL
                     KKQVRSAQKGNLTNYINTILNSKSVSDAVNRVVAIREVVSANEKMLAQQEADKAALEA
                     KQIENQNAINTVAANKQAIENNKAALATQRAQLEAAQLELSAQLTTVQNEKASLIQAK
                     AQAEEAARKAAEAQAAAEAKAQAEAKAQAESVAKAQAAAQVESATAPTETVQTQPRTE
                     IKPSNLTATSSATTVATTTATATNEPKVTQPSVVTKAVEAPKAVVSSTPRAVSKPVVR
                     SYDSSNTYPMGQCTWGAKSMASWVGNYWGNANQWGASARAAGYSVGTTPRVGAVAVWP
                     YDGGGYGHVAVVTSVANNSSIQVMESNYAGNMSIGNYRGSFNPSASGSVYYIYPN"
     gene            1802..2770
                     /locus_tag="VBE22_001808"
     CDS             1802..2770
                     /locus_tag="VBE22_001808"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO7203239.1"
                     /translation="MSYSNLKLFALSSNKELAKKVSQTIGIPLGQSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAESVSVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRQ
                     GLVGDDVVVVSPDHGGVTRARKLAQCLKTPIAIIDKRRSVTKMNTSEVMNIIGNIKGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQNSAIEKLIVL
                     DTIYLPEERLIDKIEQISIAELIGEAIIRIHEKRPLSPLFEMNKLK"
     gene            2878..4053
                     /locus_tag="VBE22_001809"
     CDS             2878..4053
                     /locus_tag="VBE22_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO7203240.1"
                     /translation="MTLEKRFNKYLDRIEVSLIRQFDQSISDIPGMVKLTLGEPDFTT
                     PDHVKEAAKSAIDANQSYYTGMSGLLALRQAAADFAKDKYNLTYNPDCEILVTIGATE
                     ALSASLIAILEAGDVVLLPAPAYPGYEPIVNLVGADIVEIDTRENDFRLTPEMLETAI
                     IQQGEKLKAVLLNYPTNPTGITYSRQEIAALAEVLKKYDIFVISDEVYSELTYTGQQH
                     VSIAEYLPNQTILINGLSKSHAMTGWRVGLVYAPEAFIAQIIKSHQYMVTAASTISQF
                     AGVEALSVGKNDTLPMRQGYIKRRDYIIDKMSKLGFKIIKPSGAFYIFAKIPDSYPQD
                     SFKFCQDFAYQQAVAIIPGVAFGKYGEGYIRLSYAASMEVIETAMARLKVFMESYEG"
     gene            4043..>4762
                     /gene="recO"
                     /locus_tag="VBE22_001810"
     CDS             4043..>4762
                     /gene="recO"
                     /locus_tag="VBE22_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEO7203241.1"
                     /translation="MRVSQTYGLVLYNRNYREDDKLVKIFTETEGKRMFFVKHASKSK
                     FNAVLQPLTIAHFILKINDNGLSYIDDYKEVLAFQETNSDLFKLSYASYITSLADVAI
                     SDNVADAQLFIFLKKTLELIEDGLDYEILTNIFEVQLLERFGVALNFHDCVFCHRVGL
                     PFDFSHKYSGLLCPNHYYKDERRNHLDPNMLYLINRFQSIQFDDLQTISVKPEMKLKI
                     RQFLDMIYDEYVGIHLKSKKFI"
BASE COUNT         1514 a          753 c          946 g         1549 t
ORIGIN      
        1 aatattaatt tggagaatta ctcaagaggc tgaagaggac ggtttgctaa atcgttaggt
       61 cgggtaactg gcgcaagggt tcgaatccct tattctccgt aaatattata attttaagag
      121 taattatact ctttttttat ttctctttta gtttcactat ggagtaatta tattgataga
      181 gtgaatagaa gaaggttatc gttttctaga tttgattatg gttcatttag aaatagttga
      241 tttcaaatat aaatgtaaca atgacgtaat ataatttcgt gaattttttg gtaaaatatt
      301 taatgtctta tcgtaaagga gtagtttaat aaaaatgaaa aaaagaatat tatcagcagt
      361 ccttgtgagt ggagtaactc ttggaactgc tgccgtaaca gtaaacgctg atgactttga
      421 ctcgaaaatt gctgctactg attcagtaat caatacttta tcagggcaac aagcagcagc
      481 acaaaaccag gtgactgcta ttaaaggaca agttggtgct ttagagtctc aacaatcaga
      541 attagaagcg caaaatgctc aacttgaggc tgtatctcag cagttaggtc aagagattca
      601 aactctttct aataaaattg ttgcacgtaa cgaatctttg aaaaagcaag ttcgtagtgc
      661 tcaaaagggt aacttgacta actatattaa tactatttta aattcgaaat cagtatctga
      721 tgctgttaat cgtgttgttg ctattcgtga ggttgtttca gctaatgaaa agatgttagc
      781 acaacaagag gctgacaaag cggccttaga agctaaacaa attgaaaatc aaaatgctat
      841 aaatactgta gcagctaaca agcaagctat cgaaaataat aaagcagcct tagcaacaca
      901 aagagcacaa ttagaagcag ctcaattaga gttatctgct caattgacta cagtccaaaa
      961 tgaaaaagca tctttaattc aggcaaaagc acaagctgag gaagctgcgc gtaaggcagc
     1021 tgaagcacaa gcagcagctg aagcgaaagc acaagctgaa gcgaaagcac aagctgagtc
     1081 tgtagcaaaa gcacaggcag cagcacaagt tgagtctgcc acagctccta ctgaaacagt
     1141 tcaaacacaa ccgagaactg aaataaagcc ttctaatctt acggctactt cttcggcaac
     1201 tactgttgct acaactacag ctacagctac taatgagcca aaagttactc aaccttctgt
     1261 agtaactaaa gcagttgaag cacctaaagc agttgtttct tcaacaccta gagcggtatc
     1321 aaaacctgtt gtcagaagtt atgattcaag taacacatac ccaatgggac aatgtacttg
     1381 gggagctaag tcaatggctt cttgggttgg taactattgg ggaaatgcta atcagtgggg
     1441 tgctagtgca cgtgcagctg gttattctgt aggaactacc cctcgtgttg gtgcagtagc
     1501 tgtttggcca tatgatggtg gtggttacgg gcacgttgca gtagtaacgt cagtggctaa
     1561 caattcatct atccaagtta tggaatcaaa ctatgctgga aatatgagta ttggcaatta
     1621 ccgtggttca tttaacccta gtgcttctgg ctctgtttac tatatttacc caaattaatg
     1681 aatactaagg agatctttcg tggtctcctt tttgtttatt aattaggtat ggtttatttt
     1741 ggattgaaaa attgtgtaaa aagcgttaaa atggtatagg actaaagttt tggaggaaat
     1801 catgtcttat tctaatttaa aattgtttgc actgtcatca aacaaagaac tagcgaaaaa
     1861 agtgtctcag acgattggaa ttccgctagg tcaatcgaca gttcgccaat tttctgatgg
     1921 tgagattcaa gttaatattg aagagtctat tcgtggccat catgttttta ttttacaatc
     1981 aacaagctca ccagttaatg ataatttgat ggaaatttta attatggttg acgctttaaa
     2041 gcgtgctagt gcggagtctg ttagtgtggt aatgccttac tatgggtatg ctcgtcaaga
     2101 tcgcaaggca cgttctcgtg aacctattac ttcgaaactc gttgcgaaca tgctagaagt
     2161 tgctggtgtt gatcgtcttt taacagttga tttacatgca gctcaaattc agggattctt
     2221 tgatattccc gttgatcacc ttatgggggc tccccttatt gcagattact ttgatcgtca
     2281 gggattggtt ggtgatgatg ttgttgtcgt tagtcctgat catggtggcg taacacgtgc
     2341 acgtaagctg gcacagtgtt taaagacacc gattgctatc attgataagc gccgtagtgt
     2401 tactaaaatg aatacatctg aagtaatgaa tattattggt aatatcaaag gtaaaaaatg
     2461 tattttaatt gatgatatga ttgatactgc aggtactatt tgccatgctg cagacgcttt
     2521 agctgaagca ggggcaacag cggtttatgc ttcatgtact caccctgttt tatcagggcc
     2581 ggctttggat aatattcaaa attcagctat tgaaaaatta attgtgttag atacgattta
     2641 cctccctgaa gaacgtttga ttgataagat tgaacaaatt tcaattgcgg aattgattgg
     2701 tgaagctatt atccgtattc acgagaaacg tcctttatca ccattatttg aaatgaataa
     2761 actaaaatga caaaaaacct gttattaaca ggttttttat catttaatga cggaaaactg
     2821 gacacaaatt atttttcttg tataattaaa tatattattt cttatcagga ggttatgatg
     2881 acattagaga aacgatttaa taagtactta gatagaatag aggtctcctt aattcgtcag
     2941 tttgatcaat ctatttcaga tatacctgga atggtaaaat taactcttgg agaaccagat
     3001 tttaccactc ctgatcatgt taaagaagca gctaaatcag ctattgatgc gaatcaatcg
     3061 tattacactg gtatgagcgg tttattagct ctaaggcaag ctgctgcaga ttttgcaaag
     3121 gataaatata atcttactta taatcctgat tgtgagatct tggttacgat aggtgccaca
     3181 gaagctctgt ctgcatctct tatagctatt ttagaagctg gtgatgttgt tctattacct
     3241 gcccccgctt atccaggata tgagccaata gttaaccttg taggagctga tatcgtagaa
     3301 attgatacta gagaaaatga ctttagacta actcctgaaa tgttagaaac ggctattatc
     3361 caacaaggtg aaaaattaaa ggctgtttta cttaattatc ctactaaccc tacaggtatt
     3421 acctattcaa ggcaagaaat tgctgcatta gctgaagtcc ttaaaaaata tgatattttt
     3481 gttattagtg atgaagttta ttctgagtta acttacactg gtcagcagca tgtttcaatt
     3541 gcagaatatt tacctaacca aactatctta attaatggct tatcaaaatc acatgctatg
     3601 actggttgga gagttggatt ggtgtatgct cctgaagcat ttattgctca aatcattaaa
     3661 agtcatcaat acatggtgac agctgctagt actatttcgc aatttgctgg ggtagaagcc
     3721 ttatctgtgg gtaaaaatga tacgttacct atgcgtcaag gatatattaa acgtagagat
     3781 tatatcattg ataaaatgtc taaacttggt ttcaaaataa ttaaaccgag tggcgctttt
     3841 tatatctttg ctaaaatacc tgatagctat ccacaggatt ctttcaagtt ttgtcaggat
     3901 tttgcttatc aacaagctgt agctattatt ccaggagtgg cttttggtaa atatggtgaa
     3961 ggatatatac gtctttctta tgctgctagt atggaagtta ttgaaacagc aatggctaga
     4021 ttgaaagttt ttatggaatc atatgagggt tagtcaaaca tacggtctcg ttttgtataa
     4081 tcgtaattat cgtgaagatg ataaattagt taaaatcttt acggagactg aggggaaaag
     4141 gatgtttttt gtaaagcatg cttcaaagtc aaaattcaat gctgtgctcc aacctttgac
     4201 tattgctcat tttattttaa aaattaatga taatggtctt tcttatattg atgattataa
     4261 agaagtttta gcatttcaag aaaccaattc agacttgttt aagttgtcat atgcgagtta
     4321 tattacttct ttggctgatg tggctattag tgataatgta gcggatgctc aattatttat
     4381 tttcctaaag aaaacgttag aattgattga ggacggttta gattatgaga ttctaacgaa
     4441 tatttttgaa gtacaactac tagagaggtt cggtgttgct ttaaattttc atgattgtgt
     4501 tttttgtcat agggtaggat taccttttga tttttcacac aaatattctg gattattatg
     4561 tccaaaccat tattataaag acgagagaag aaaccaccta gatcctaata tgctgtactt
     4621 aatcaatcgt tttcagtcaa ttcaatttga tgatttacaa acaatttctg tgaaacctga
     4681 gatgaaactt aaaattcgtc aatttttgga catgatttac gatgaatatg tagggattca
     4741 tcttaaaagt aaaaaattta tt
//
LOCUS       DATRTN010000108         4762 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.108, whole genome shotgun sequence.
ACCESSION   DATRTN010000108 DATRTN010000000
VERSION     DATRTN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.108"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..971)
                     /locus_tag="VBE22_001811"
     CDS             complement(<1..971)
                     /locus_tag="VBE22_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203242.1"
                     /translation="MLDLEKILRGMISAKFKDLRGSIPPDIISNGQRTAIYNIEKGKN
                     PKSGNFVTDTLLEDYSSYFGMDKSDLIFGDALLLELTLYHVFSQIFYQIVPDDNRIGL
                     KIDRNKIQNNIDSSIIEPFLELFYIFGDFGRWYNIRRKDDDESDKDIDYTTMYEIVWR
                     LIKSRVVSSFNQHVVGEMFEKDKVFNFNQINNVFNNWYHKHFVKIIIPEALKKLESDS
                     IFKMGFMVRNLIENFLVLDLPLSYLEDVPLEEYYLPITNYTIDISKIESEEVENKVLR
                     EYLRWILRYNSLETSDDAKKFADENFFEEFDFVTEERRPLVDQTTKI"
     gene            complement(1023..1514)
                     /locus_tag="VBE22_001812"
     CDS             complement(1023..1514)
                     /locus_tag="VBE22_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203243.1"
                     /translation="MAKSLQSQFRDIERNLVRETNKMVKRANKKIKPVILPTQLDSSK
                     TALNFNEYHQDNSTHVSVNGDLINSQIGGQNNALNITYTEIEIERVFENIREYSRTLD
                     EEEQTELVTILNEVQEVGKVDNHQTFFEKHPILAMALSASVSWGVENGIDRLVSVISN
                     TFK"
     gene            complement(1528..>4762)
                     /locus_tag="VBE22_001813"
     CDS             complement(1528..>4762)
                     /locus_tag="VBE22_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEO7203244.1"
                     /translation="FNKNLEIKKCIITNGLSWFVIEAKEFEKHFFKNKKLVDLVTKFR
                     NNQLSSNKTDFLYSEVIAPEIDKAFEKGIVIAHFDLSQALVKTSKSIEIKKNNLTQLY
                     RFFTAENLLNKEIFTDSNKLNKNFYDELLYLMGLEETKSGTSKIISRLKPIKRQRYSF
                     VENIINKLEMKDVSKEKQEDIAIQLTVVWTNRILFLKLLESQLVLFNKDESYRFLTYE
                     KLPNFEEIYGLFFAVLAKKVSERNDRVQEKFGYVPYLNSSLFEETEIEISRDGIGIDR
                     LPEGDIEIFSKTALKGVDKKRKKGNINFIEYLFEFLDSYDFSTSISHHEKSKNDLINA
                     SVLGLIFEKINGYRDGSFYTPGNITMYMSRKAIRTAAVDKVNELLGWNCETVEEIKFA
                     IGHSVENARKVSQAIDDLKVCDPAVGSGHFLVSILNEIIALKSDLNILFDADGNYIGN
                     LIQCYVINDELVIQDMSGNNFLYQTGNQQSEQIQKAIFNQKRHILENSLFAVDINPSS
                     VNICRLRLWIELLKSSYYYQDTDTNQPVLTTLPNIDINIKVGDSLLHKFEFDYEFDMR
                     KTDFKDYLSLVKDYKETNNKRVKAEIWEKIERLKHSFDDTASSPELKKLSRLISERNK
                     AGAISLFEDENKNSKEVAELNKQLAKAEKELEYRLKNPLFAKGMEWRMEFPEILGEAG
                     EFIGFDLVIGNPPYIFARNQSFTDEMKSYYSRTYQVSEYQANTYTIFMELAYQLLRKG
                     GTFSYIIPNNFLTIQTNSRVRQFITEQTSDVVLINSLDKIFADASVDNCIIFFKKNAP
                     NWIEVAELYHGEFNTVGRVAPDFFGEIPTFSISMVKYRQAIDIFWKVNHFPILGRPEI
                     STVKSGIKAYETGKGNPKMTAQDKNNRIYHARERSDDSYRPYLDGENVSRYKLTWNGE
                     YVKYGENLAAPRDKEIFEKPRILVRQIPSKSTYAVEAVYTDSDVINDLNSMVITDILV
                     NPFYLLGILNSRLISLWFFMKFDKFQRRLFPQFKVNELGDFPIPDATDSQQEDIAQVV
                     EQLMEEMKKDSPDTEKVNQLNLEIDELVMDLFDLAEEEKQTVRDFEV"
BASE COUNT         1473 a          912 c          697 g         1680 t
ORIGIN      
        1 tttattttag tggtttggtc aactaatgga cgcctttctt cagtaacaaa atcaaattct
       61 tcaaaaaaat tttcatcagc aaatttcttt gcatcatcag atgtttctaa gctgttatat
      121 cttaatatcc atcttaaata ttctcttaaa actttattct ctacttcttc agattctatc
      181 tttgaaatat ctatggtata gttcgttatt ggtaagtagt attcctctaa tggaacatct
      241 tctaaatatg atagtggtaa atctaaaacc aaaaaatttt caatcaaatt cctaaccata
      301 aatcccatct taaaaatact gtcagactcc aattttttta acgcctcagg gattatgatt
      361 tttacaaaat gcttatgata ccaattatta aagacattgt tgatttgatt aaaattaaat
      421 accttgtctt tttcaaacat ttctccaact acatgttgat taaatgaact aaccactcta
      481 ctttttatca aacgccaaac gatttcatac attgtcgtat aatctatatc tttatctgat
      541 tcatcatcat ccttccttct aatattatac catcgcccaa aatccccaaa aatatagaat
      601 agttctaaaa atggctcaat aatgcttgaa tcaatattgt tttgaatttt gttcctatca
      661 atcttcaagc caatacgatt atcatctggt actatttgat aaaaaatttg agaaaataca
      721 tggtaaagag tcagttctaa taatagagca tctccaaaga taagatctga tttatccatt
      781 ccaaaatagc tagagtaatc ctccagtaaa gtatcggtta caaaatttcc gcttttagga
      841 tttttacctt tttcaatgtt gtagattgca gttctttggc catttgaaat gatatctggt
      901 ggaatgcttc ccctcaagtc cttgaatttt gcagaaatca ttcccctcaa aattttctct
      961 aagtccaaca tcatctacac ctcaaactct ccattttcta tttccatcaa ataaaaaact
     1021 tttcacttaa atgtatttga aatgacacta actaatctat caattccatt ctccactccc
     1081 cagcttacac tagcactaag agccattgca agtatagggt gtttttcaaa aaaagtttga
     1141 tggttatcaa cttttccgac ttcttgtacc tcatttaaaa ttgtcactaa ttctgtctgt
     1201 tcttcttcat ctaatgttct agagtactct ctgatattct caaatactct ctcaatttct
     1261 atctcagtat aggtaatatt taacgcatta ttttgtccac caatctgtga gtttatcaaa
     1321 tctccattaa cagaaacatg agtactatta tcttgatgat attcgttaaa gtttagtgct
     1381 gttttagagg aatctagttg tgttggaagg ataacaggtt ttatcttctt attagctctc
     1441 ttcaccattt tattcgtttc tctgactaaa tttctctcaa tatctctgaa ttgactttgc
     1501 aaactttttg ccatttacac ctactttcta tacttcaaaa tctctaactg tttgtttttc
     1561 ttcttcagcc aagtcaaaca agtccatgac aagttcatca atttccagat taagctgatt
     1621 gactttttca gtgtcaggag aatctttttt catctcctcc atcagttgct caacaacttg
     1681 tgctatgtct tcttgctggg aatcagtagc atctggaatt ggaaaatctc ctagttcatt
     1741 aactttgaac tgtgggaata ggcgacgttg gaatttatca aacttcatga aaaaccaaag
     1801 agagattaag cgagagttca aaatacccaa aagataaaaa ggattaacca gaatatctgt
     1861 gatgaccatg gagtttaagt cgttaataac atcactatca gtataaacag cttcaacagc
     1921 ataagttgat ttacttggaa tctgacgaac taaaattcgt ggtttttcaa aaatttcttt
     1981 atctcttggt gcagctaaat tttcaccata cttgacatat tcaccattcc aagtcagttt
     2041 atagcgtgat acattctcgc catcaagata gggacggtat gaatcatctg acctttctct
     2101 tgcatgataa attcgattat tcttatcttg agcggtcatt ttaggattcc cttttccagt
     2161 ttcataggct ttgattccag attttacagt cgaaatttct ggtcgtccta gtattggaaa
     2221 atggtttact ttccagaaga tatcaatcgc ctgacgatac ttgaccatcg agattgaaaa
     2281 ggtagggatt tcaccaaaga aatctggcgc aactcgtcca acagtgttga actcgccgtg
     2341 atacaattct gctacttcaa tccagtttgg cgcattcttc ttgaagaaaa tgatacagtt
     2401 atccacgcta gcatccgcaa aaatcttatc cagagaatta atcagaacaa catcactcgt
     2461 ctgctccgtg ataaactgac gaactcttga attggtctga atggtcagaa aattatttgg
     2521 aatgatataa gagaatgtcc cacctttacg taagagttga taagccaatt ccatgaatat
     2581 agtataggta ttggcttggt attcacttac ttgataagtc cttgaatagt aggatttcat
     2641 ctcatctgta aaggactgat tacgagcaaa gatataaggc ggattcccaa ttacaaggtc
     2701 aaagccgata aattcgccag cttctcccaa gatctcagga aattccattc gccattccat
     2761 acctttagcg aataatggat ttttcagtct gtattccaat tccttttcag cttttgctag
     2821 ttgcttattt aattccgcta cttctttaga atttttattt tcatcctcaa ataagctaat
     2881 ggcaccagcc ttatttcgtt cagatataag ccgagataat ttctttaatt cagggcttga
     2941 agctgtgtca tcaaaggaat gtttgagcct ttcgattttc tcccagattt ctgctttaac
     3001 tcgtttatta ttcgtctcct tatagtcttt aactagagat agatagtcct tgaaatctgt
     3061 ttttctcata tcaaattcat agtcaaattc aaacttatgt aagagactat cgcctacttt
     3121 aatattgata tcaatatttg gcaaagtcgt caaaacaggt tggttggtat cagtatcctg
     3181 gtagtaataa gaagatttta agagttcaat ccaaagacgc aaacggcaaa tattgaccga
     3241 gcttgggtta atatccactg caaagagcga attttccaga atatgccttt tctggttgaa
     3301 aattgccttt tgaatttgtt ctgactgctg attacctgtc tgatacaaaa aattatttcc
     3361 agacatatct tgaatgacta gctcatcatt gataacataa cattgaatca ggtttccaat
     3421 atagttgcca tcagcgtcaa acaaaatatt taaatcactt ttaagagcaa taatttcatt
     3481 caggattgaa acaaggaagt gaccggatcc aaccgctggg tcacaaacct taagatcatc
     3541 aattgcttgg ctgactttac gagcattttc aacactatgt ccaatcgcaa acttaatctc
     3601 ttcaacagtt tcacaattcc aaccaagcaa ttcattaact ttgtccactg ctgctgtacg
     3661 aatcgcttta cgtgacatat acattgtaat gttacctggc gtataaaaag agccatcacg
     3721 gtagccgttg attttctcaa aaattaatcc cagaactgac gcattgataa ggtcattctt
     3781 agatttttca tggtggctga tggaggtcga gaagtcataa gaatccaaaa actcaaagag
     3841 atattcaata aaattgatgt tccctttttt acgtttttta tctacgcctt taagggctgt
     3901 tttggagaaa atttcaatat ctccctctgg taatctatca ataccaatcc catctctaga
     3961 aatttctatt tctgtttctt caaacagtga tgagttcaga taagggacat aaccgaactt
     4021 ttcttgcact cgatcatttc tttccgatac tttcttagca agtacagcaa agaatagacc
     4081 gtaaatttct tcaaaattag ggagtttttc atacgtcaag aacctataag attcatcttt
     4141 gttaaagagt acaagctgag attctaacag tttcaaaaat agaattctat ttgtccagac
     4201 aactgttagt tgaatagcaa tatcttcctg cttttctttt gaaacatctt tcatctccaa
     4261 tttattaatg atattctcaa caaaagaata tcgctgacgt ttgatgggtt ttaagcgtga
     4321 aataattttt gaagtccctg acttcgtttc ttccagcccc atcaaataaa gcaattcatc
     4381 atagaaattc ttattgagtt tgtttgaatc agtaaaaatc tctttattga ggagattctc
     4441 tgctgtgaag aatcgataaa gctgagtcag attatttttc ttgatttcaa tagacttact
     4501 ggtcttaact agagcttgac taagatcaaa gtgagcaata acgattccct tttcaaatgc
     4561 tttgtcaatt tctggtgcaa taacctcact ataaaggaaa tctgttttat tgctagaaag
     4621 ttgattattt ctgaactttg tcacgaggtc aactagcttt ttatttttga aaaaatgctt
     4681 ttcaaattct ttagcctcta ttacaaacca agatagaccg tttgtgataa tacacttttt
     4741 gatttctaaa tttttattaa at
//
LOCUS       DATRTN010000109         4760 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.109, whole genome shotgun sequence.
ACCESSION   DATRTN010000109 DATRTN010000000
VERSION     DATRTN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.109"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(22..780)
                     /locus_tag="VBE22_001814"
     CDS             complement(22..780)
                     /locus_tag="VBE22_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284767.1"
                     /note="The term CAMP (Christie, Atkins, Munch-Petersen)
                     factor is used for toxins found in group A and B
                     Streptococcus. Related toxins are also found in
                     Propionibacterium acnesother and other bacterial species;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor family pore-forming toxin"
                     /protein_id="HEO7203245.1"
                     /translation="MAKKQVVYFSIAILTGVALFSPAILEVHADQATNHVVVSQVNNQ
                     QQIKQKLDQDSVQLNTIKNQVQGTDYEKPVDTAINAVDKMKSSLGGNPATIYDLGSIG
                     PRIEAITDVIEAINFSTHNLTNKVSQANVDMGFGITKLVIRIADPFASVDSIKAQVAE
                     VKQLEQKVMTYPDLKPTDKATIYTKSKLDKAIWDIRVQRDQKVLNKKSFDVYNALNKA
                     ITHAVGVQLNPNVTVQEVDQEIVNLQMALQTALK"
     gene            complement(1077..2384)
                     /locus_tag="VBE22_001815"
     CDS             complement(1077..2384)
                     /locus_tag="VBE22_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEO7203246.1"
                     /translation="MSDLLSLPDIKTIEPPQENETDMMFKVEAVGPPERCPECGFDKL
                     YKHSSRNQLIMDLPIRLKRVGLHLNRRRYKCRECGSTISVDEKRSMTKRLLKSIQEQS
                     MSKTFVEVAESVGVDEKTIRNVFKDYVALKEREYQFETPKWLGIDEIHIIRRPRLVLT
                     NIERRTIYDIKPNRNKETVIQRLSEISDRTYIEYVTMDMWKPYKDAVNTILPQAKVVV
                     DKFHVVRMANQALDNVRKSLKAHMSQKERRTLMRERFILLKRKHDLNERESFLLDTWL
                     GNLPALKEAYELKEEFYWIWDTPDPDEGHLRYSQWRHRCMSSNSKDAYKDLVRAVDNW
                     HVEIFNYFDKRLTNAYTESINSIIRQVERMGRGYSFDALRAKILFNEKLHKKRKPRFN
                     SSAFNKAMLYDTFNWYEVNDHDITDNLGVDFSTLIKNLEKGDL"
     gene            complement(2544..4184)
                     /gene="merA"
                     /locus_tag="VBE22_001816"
     CDS             complement(2544..4184)
                     /gene="merA"
                     /locus_tag="VBE22_001816"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HEO7203247.1"
                     /translation="MNKFKVNISGMTCTGCEKHVESALEKIGAKNIESSYRRGEAVFE
                     LPDDIEVESAIKAIDEANYQAGEIEEVSSLENVALINEDNYDLLIIGSGAAAFSSAIK
                     AIEYGAKVGMIERGTVGGTCVNIGCVPSKTLLRAGEINHLSKDNPFIGLQTSAGEVDL
                     ASLITQKDKLVSELRNQKYMDLIDEYNFDLIKGEAKFVDASTVEVNGTKLSAKRFLIA
                     TGASPSLPQISGLEKMDYLTSTTLLELKKIPKRLTVIGSGYIGMELGQLFHHLGSEIT
                     LMQRSERLLKEYDPEISESVEKALIEQGINLVKGATFERVEQSGEIKRVYVTVNGSRE
                     VIESDQLLVATGRKPNTDSLNLSAAGVETGKNNEILINDFGQTSNEKIYAAGDVTLGP
                     QFVYVAAYEGGIITDNAIGGLNKKIDLSVVPAVTFTNPTVATVGLTEEQAKEKGYDVK
                     TSVLPLGAVPRAIVNRETTGVFKLVADAETLKVLGVHIVSENAGDVIYAASLAVKFGL
                     TIEDLTETLAPYLTMAEGLKLVALTFDKDISKLSCCAG"
     gene            complement(4213..4605)
                     /gene="merR"
                     /locus_tag="VBE22_001817"
     CDS             complement(4213..4605)
                     /gene="merR"
                     /locus_tag="VBE22_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HEO7203248.1"
                     /translation="MIYRISEFADKCGVNKETIRYYERKNLLQEPHRTEAGYRIYSYD
                     DVKRVGFIKRIQELGFSLSEIYKLLGVVDKDEVRCQDMFEFVSKKQKEVQKQIEDLKR
                     IETMLDDLKQRCPDEKKLHSCPIIETLT"
BASE COUNT         1397 a          956 c          718 g         1689 t
ORIGIN      
        1 gcgttctggt ttagtatcaa ctcatttcaa agcagtttga agtgccattt gtaggttcac
       61 aatctcttga tccacttctt gaacggttac attaggattt aattggacgc ctactgcatg
      121 tgtgatagct ttatttaaag cattataaac atcaaaactt ttcttgttta aaactttttg
      181 gtctctttgt acacgaatat cccaaattgc tttgtccagt tttgacttag tataaatagt
      241 tgctttatct gttggcttca aatctggata agtcattact ttttgttcta attgcttcac
      301 ttctgctact tgtgctttta tagaatcaac agatgcaaat ggatcagcaa tacgaataac
      361 taatttagtt attccgaatc ccatgtcaac attagcttga ctaactttgt tagtcaagtt
      421 atgtgttgaa aaattaattg cttcaataac atctgtgata gcttctatac gtggaccaat
      481 agaaccaaga tcgtagattg tcgctggatt accacctaaa gaagacttca tcttatcaac
      541 agcatttata gcagtatcta caggtttttc ataatctgtg ccttgaactt gatttttaat
      601 agtattcaat tgaacactat cttgatcaag tttttgtttt atttgttgtt gattattaac
      661 ttgactaact acgacatgat tagtagcttg atcagcatga acttccaaaa tcgctggaga
      721 aaacaatgca actcctgtca aaatagctat ggaaaaatag actacttgtt tctttgccat
      781 ggtttttccc tcctaaatca aaataactta atatactgca gtatttttaa cttgtaggta
      841 cctacaccag tagtatacag tctaaaaaaa atactgtcaa acctaattta aaatataatt
      901 ctattgaaaa tcatataatt ccacttattt tttaatgaga ttaaatattt tatttttata
      961 taatataata aaatcctata aaatataatt ttcagacata ttagaattag ctataactat
     1021 aaaagcaact attatctttt taaattgggt attcggattt tatggtggaa aagggcttat
     1081 aaatcaccct tctccaaatt cttaataagt gtggaaaaat cgacacctaa gttgtctgta
     1141 atgtcgtgat cattcacttc ataccaattg aaagtatcgt ataacatagc tttattgaaa
     1201 gcacttgaat taaatcgtgg cttacgcttt ttatggagct tctcattgaa aaggattttg
     1261 gctcgtaagg catcaaacga gtaacctcta cccattcgct ctacctgcct aataatgctg
     1321 ttaattgact ccgtataagc attagtgagc cttttatcaa agtagttgaa tatttcaaca
     1381 tgccagttgt ctacggctct cacgaggtct ttatatgcgt ctttagagtt gctggacata
     1441 caacggtgtc tccattgact ataacgaaga tgaccttcat ctggatcagg agtatcccat
     1501 atccagtaaa actcttcttt gagttcatag gcttctttta aagcaggaag attacctaac
     1561 caagtatcta agaggaatga ttcacgttca tttagatcgt gtttacgctt tagaaggata
     1621 aacctttcac gcataagggt acgtctttct ttttggctca tatgggcttt caaagacttt
     1681 ctgacgttat ctaaggcttg attagccatt ctaactacat gaaacttatc tacgacaact
     1741 ttagcttgtg gaaggatagt gttcactgcg tctttgtagg gcttccacat atccattgtg
     1801 acgtactcaa tgtaagtcct gtcactgatt tctgaaagac gttggatgac tgtttccttg
     1861 ttacggttag gcttgatgtc ataaatagtc ctgcgttcaa tattagtcaa tacaagccga
     1921 ggtctacgga taatatgtat ctcgtctatc ccaagccact taggagtttc aaactggtat
     1981 tcacgttctt tgagtgccac atagtcctta aaaacgttcc taatggtttt ctcgtcaaca
     2041 ccaacgcttt ctgcgacttc tacaaaggtc ttagacatgg attgctcttg aatggacttt
     2101 aaaagccttt tggtcatact acgcttttca tctacagata tggtagatcc gcattcacga
     2161 cacttgtatc gtctacggtt caaatgtaag cccactcgct ttaaacgaat gggcaaatcc
     2221 ataattagtt gatttcttga actgtgtttg tacaacttgt caaaaccaca ttcaggacaa
     2281 cgttcaggtg gtccgactgc ttcaacttta aacatcatat cggtttcatt ttcttgtggc
     2341 ggttctattg ttttaatgtc tggtagggat aataagtctg acatattatt catcctttat
     2401 tactctgcgg tgttaaggta tcaattatag atttgatgtc gaaatcatca gtgttaaatt
     2461 atttttgtga ccacaaatgc acttagaaaa tcccttaaag cattagctgg ttcacttgaa
     2521 tagggagttg taaaaaagtt cccttagcct gcacaacaag ataatttcga aatatcttta
     2581 tcgaacgtaa gtgcaactaa ttttagccct tcagccattg ttaaatatgg tgctagggtt
     2641 tctgttaaat cttctatcgt taagccaaat ttaacagcta atgatgctgc atagatgaca
     2701 tctcctgcat tttcagatac aatatgaacc cctaatactt ttagtgtttc tgcatctgct
     2761 actagtttaa atacaccggt tgtttcacgg tttacaattg ctcttggaac agcacctaaa
     2821 ggtaatacag atgtcttcac atcataccct ttctcttttg cttgttcttc tgttaaacca
     2881 accgttgcaa ccgtcggatt cgtaaacgta acagcaggaa ctaccgataa atctattttt
     2941 ttgtttaatc caccaatagc attatcagta ataattccac cttcataggc tgctacatat
     3001 acaaattgtg gtcctaaagt cacatctcct gctgcataaa tcttttcatt acttgtttga
     3061 ccaaaatcat tgatcaggat ttcattattt tttccagttt caacacctgc tgcacttaaa
     3121 tttaaagaat ccgtatttgg ttttcttcca gtggcaacaa gtaactgatc tgattcaatg
     3181 acttctctac tgccatttac tgttacgtaa acccttttta tctctccact ttgttcaaca
     3241 cgctcaaaag ttgccccttt gacaaggttt ataccctgtt caattaacgc tttttcaact
     3301 gactctgaaa tctcaggatc atactccttt aaaagtcgct cacttctttg cataagcgtt
     3361 atttctgaac ctaaatgatg aaatagttgt ccaagctcca ttccaatgta tcctgaacca
     3421 attacagtta atcgttttgg tattttcttt aactcaagaa gtgttgtact agttaaatag
     3481 tccatttttt caagtcctga aatttggggc aacgaaggcg atgcacctgt tgcaattaaa
     3541 aagcgttttg cagataactt tgtcccattg acctcaaccg tactagcatc aacgaatttt
     3601 gcttcacctt taattaaatc aaaattatat tcatcaatta aatccatata tttttgattc
     3661 cgaagttcgc tcaccaattt atccttttgc gtgattaaac tagctaaatc cacttctcca
     3721 gcggatgttt gtaaacctat aaacggattg tcttttgata aatgattgat ttcccctgcc
     3781 ctaagaagag tttttgacgg aacacagcca atattcacac aggttccccc aaccgttcca
     3841 cgctcaatca ttccaacttt tgcaccgtat tctatagctt taattgccga agaaaaggca
     3901 gcagcaccag aaccaataat aagaaggtca taattgtctt cattaattaa cgccacgttt
     3961 tctagtgatg atacttcttc aatttctccg gcttggtaat ttgcttcatc aatcgccttt
     4021 attgcacttt caacctcaat atcatcgggc agttcaaata ctgcttcacc acgacgataa
     4081 ctagactcaa tatttttagc acctatcttt tcaagtgctg attctacgtg tttttcacaa
     4141 cccgtacaag tcattcctga aatgtttacc ttaaatttat tcataaaaaa gctcctttcg
     4201 ttaattaatc tctcatgtta atgtttctat tattggacac gaatgtaatt tcttttcatc
     4261 tggacatcgt tgttttaagt cgtctaacat agtttcaatt cgttttaaat cctctatttg
     4321 tttttgcact tccttttgtt ttttagaaac aaattcgaac atatcttgac aacgaacttc
     4381 atctttatct acaacaccaa gtaatttata aatctcgctt aaagagaaac caagttcctg
     4441 tattcgttta ataaacccaa cacgcttaac gtcatcatat gaatatatcc gataaccagc
     4501 ttccgttcgg tgaggttctt gtaataaatt ttttcgctcg taatatctga tcgtttcttt
     4561 attaactcca catttatctg caaactcact aatgcgataa atcatcttat ttcaccccct
     4621 gaatattata aaccatgtac catagtacac ggtcaagtaa attggactat tttatttttt
     4681 tcccaaagta tcactcgtat gtgattgata cttttatata atactattga ttatcctaac
     4741 atcccaccag aaaatccgat
//
LOCUS       DATRTN010000110         4600 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.110, whole genome shotgun sequence.
ACCESSION   DATRTN010000110 DATRTN010000000
VERSION     DATRTN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.110"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(10..885)
                     /gene="tehB"
                     /locus_tag="VBE22_001818"
     CDS             complement(10..885)
                     /gene="tehB"
                     /locus_tag="VBE22_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEO7203249.1"
                     /translation="MVNTKTLLPYKTMPVWTAQSIPKAFLEKHNTKEGTWAKLTILSG
                     SLVFYQLSPDGEEISRHIFDASSDIPFVDPQVWHKVSPNSPDLSCYLTFYCQKEDYFH
                     KKYGLTRTHSEVIASAPLLSEKSNILDLGCGQGRNSLYLSLLGHQVTSVDSNGQSLVA
                     LENMALEEELPYNIKRYDINTAAIEGHYDFILSTVVFIFLNPDCISDIILQMQSHTQI
                     GGYNLIVSAMDTAENPCPLPFPFTFKEGQLKSYYNDWEIIKYNENLGELHRVDENGNR
                     LKLQFATLLARKLRS"
     gene            1028..3025
                     /gene="metG"
                     /locus_tag="VBE22_001819"
     CDS             1028..3025
                     /gene="metG"
                     /locus_tag="VBE22_001819"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEO7203250.1"
                     /translation="MTEKKSFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGFD
                     VQYLTGLDEHGQKIQQKAEEAGITPQEYVDGMAESVKTLWELLDISYDKFIRTTDTYH
                     EEAVAKIFEQLLAQGDIYLGEYTGWYSVSDEEFFTESQLAEVYRDENGNMIGGVAPSG
                     HEVEKVSEESYFFRMSKYADRLKAYYAEHPEFIQPDGRMNEMLKNFIEPGLEDLAVSR
                     TTYTWGVQVPSNPKHVIYVWIDALMNYISALGYGWSDDLSQYHKFWPADIHMIGKDIL
                     RFHSIYWPIMLMALDLPLPKRLVAHGWFVMQDGKMSKSKGNVVYPEMLVERFGLDPLR
                     YYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTIAMVNKYFDGEVPRFAVAT
                     DFDADLASVATDSIENYHKQMEAVDFPRALEAVWNLISRTNKYIDETAPWVLAKDETD
                     RDKLAAVMSHLVASLRVVAHLIQPFMMETSDAIMEQLGLGATFDLEKLTFADLPEGVR
                     VVAKGSPIFPRLDMEDEITYIKEQMNAGKAPVEKEWVPEEVELTSSKGQIKFEDFDAV
                     EIRVAEVIEVEKVEGSDKLLRFRLDAGDEGHRQILSGIAKFYPNEQELVGKKLQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTVLTVDSAVANGSIIG"
     gene            3221..4558
                     /gene="brnQ"
                     /locus_tag="VBE22_001820"
     CDS             3221..4558
                     /gene="brnQ"
                     /locus_tag="VBE22_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO7203251.1"
                     /translation="MVKKGFLTGLLLFGIFFGAGNLIFPPALGVASGQDFWPAILGFC
                     LSGVGLAIITLLLGTLTNGGYKTEMSEKFSPWFSLTFLVILYLTIGPLFAIPRTATVS
                     FEIGVAPIVGHSPIALLCFTACFFAAAYYLAIRPNGILDSVGKILTPVFAFLILSLVV
                     VGAIAYGNLESAKASADYAGKAFGSGVLAGYNTLDALAAVAFCLVATETLKKFGFKTK
                     KEYLSTIWIVGIVTSLAFSILYIGLGFLGNKFPVPADILADPNVNKGAYVLSQASYKL
                     FGNFGRYFLSIMVTLTCFTTTVGLIVSVSEFFDKNFRFGNYKLFATVFTLIGFLIANL
                     GLNAVITFSVPVLTLLYPIVIVIVLIILINKWLPLSKKGMSLTIGLVTLVSFVEVLAG
                     QWQEKTLTQLVGFLPFHTISMGWLVPMLIGIVFSLVLSDKQKGQAFDLEKFEG"
BASE COUNT         1387 a          730 c          931 g         1552 t
ORIGIN      
        1 tactcacttt tatgatctta attttcgagc taataaagtc gcaaactgta attttaagcg
       61 attaccgttt tcatccaccc tgtgcaattc acctaaattt tcattatatt tgatgatttc
      121 ccagtcattg taataagatt tcaattgtcc ttctttgaag gtaaaaggga atggaagcgg
      181 gcaaggattt tcagcggtat ccatagcaga aacgataaga ttgtatcctc caatctgagt
      241 atgtgattgc atttgtaaaa taatatcaga aatgcagtcg ggatttaaaa atataaatac
      301 cacagttgat aaaataaaat catagtgccc ttcaatagca gcagtattaa tatcatacct
      361 ttttatattg taaggaagct cttcttctaa tgccatattt tctaaagcta caaggctctg
      421 tccgtttgaa tcgacagaag tcacttgatg tcccagcagc gataaataaa gtgagtttcg
      481 cccttgacca cacccaaggt ctaatatatt actcttctca gataagagag gtgcactggc
      541 gataacctca gaatgtgtgc gcgtgagacc atatttttta tggaagtaat cttctttttg
      601 gcagtaaaaa gttagatagc aacttaagtc tggactattc ggcgaaactt tatgccagac
      661 ttgtggatca acaaaaggaa tatcactact agcatcaaaa atatgccgcg agatttcctc
      721 tccatcagga gataactggt aaaatactaa agaaccactt agaatggtta gttttgccca
      781 ggtgccttcc ttagtattat gcttttctaa aaatgccttt ggaatagact gtgcagtcca
      841 aaccggcata gttttgtagg ggagaagtgt ttttgtatta accatatatt aactctctat
      901 ttttaatacc tttattatag caaaagtaga tatattattc agagatatat ccagttgaaa
      961 aagattttat gttataatgt atcatgataa aaaacacctc tcattagggt ggcttatagg
     1021 agaaatcatg acagaaaaaa aatcatttta cattactacc ccaatttatt acccatcagg
     1081 taaattgcac atcggttctg cctatacaac gatagcatgt gatgttttgg cacgttacaa
     1141 acgcatgatg ggctttgatg ttcagtattt gactggtctt gatgagcatg gtcagaaaat
     1201 tcaacaaaag gctgaagaag caggcattac accacaggaa tatgttgatg gcatggctga
     1261 aagtgtcaaa acattatggg aacttttaga tatttcctat gacaaattca tccgtacaac
     1321 agatacttac catgaagaag cggttgctaa aatttttgaa caattgttgg cacaagggga
     1381 catctatctt ggtgagtata ccggatggta ttctgtttca gatgaagaat tttttactga
     1441 aagccaatta gcagaagttt atcgtgacga aaacggtaat atgattggtg gtgttgcgcc
     1501 atctggtcat gaagttgaaa aagtttctga agaatcttat ttcttccgca tgagcaaata
     1561 tgcagatcgt ttaaaagcgt actacgctga gcatccagaa tttattcagc cagatggtcg
     1621 tatgaatgaa atgcttaaaa actttataga accaggttta gaagatttag cagtgtctcg
     1681 tacaacttac acatggggag ttcaggtacc atcaaatcca aaacatgtta tttatgtctg
     1741 gattgatgct ttgatgaact atatttctgc tcttggttat ggttggtctg acgatctcag
     1801 tcaatatcat aaattttggc cagcagacat tcatatgatt ggtaaagata ttcttcgttt
     1861 ccactctatt tattggccta tcatgttgat ggcgctggat ttgccattac caaaacgtct
     1921 agttgctcat ggttggtttg tgatgcaaga tggtaaaatg tctaaatcaa aagggaatgt
     1981 cgtttatcca gaaatgttag tagagcgttt tggtcttgat ccactccgtt attatctcat
     2041 gcgttcattg cctgttggtt cagacggaac ttttacgcca gaagattatg ttggacgtat
     2101 taactatgaa cttgctaatg atttaggaaa ccttctcaac cgcactattg ctatggttaa
     2161 caagtatttc gatggagaag taccaaggtt tgctgttgca actgactttg atgccgattt
     2221 agcttctgtt gcgacagata gtattgaaaa ctatcataaa caaatggaag cggtggattt
     2281 cccacgtgcc cttgaagctg tatggaatct tatttcacga actaacaaat atattgatga
     2341 aacagcaccg tgggtacttg caaaagacga aacagatcgt gataagttag cagctgtaat
     2401 gagtcattta gttgctagtc tacgtgttgt tgctcacctc attcaaccgt tcatgatgga
     2461 aacttccgat gccattatgg aacaacttgg tttaggggca acattcgatc ttgaaaaatt
     2521 gacttttgct gatctaccag aaggtgttcg agttgttgca aaaggttcac caattttccc
     2581 aaggttggat atggaagacg aaattactta tatcaaagaa caaatgaatg ctggaaaagc
     2641 accagtggaa aaggagtggg ttcctgaaga agttgaattg acatcaagca aaggtcaaat
     2701 caagtttgag gattttgacg ctgttgaaat ccgcgttgca gaagtaattg aagttgagaa
     2761 agtggaaggt tctgataaac ttcttcgttt ccgtctagat gctggtgatg agggccatcg
     2821 tcaaatctta tcagggattg caaaatttta cccaaatgaa caagagttag tagggaaaaa
     2881 actccaaatt gttgctaacc tcaaaccacg taaaatgatg aaaaaatatg tcagtcaagg
     2941 catgattctc tcagcagaac atgatggtaa attgacagtg ttgacggtcg attcagcagt
     3001 ggcaaatgga tcaattattg gttaatcgaa aagagcatta tgctcttttt ttgttttcaa
     3061 gaaaaaagtg actgaatagt tggaaaatta tgaaaatgac ctagaattta tttttatttt
     3121 tgatataata aaagacatcg attctaagca tttagataga atttgataag gagaatttat
     3181 ggaaagggtg taaatagatt atttacacta atggttgctt atggtaaaaa aaggcttttt
     3241 aacgggatta ctcttatttg gtatcttctt tggtgcaggg aacctcattt ttccaccagc
     3301 attaggtgta gcatctggac aagatttctg gccagcgatt ttaggatttt gtttgtcagg
     3361 agttggatta gctattatta ccctcctact aggcacacta actaatggtg gttataagac
     3421 agagatgtcg gaaaaattta gtccgtggtt ttctttaaca tttttagtta tactctacct
     3481 aactattggt ccactttttg ctattcctag aacagcaacg gtttcttttg aaattggggt
     3541 tgctccaatt gtagggcata gtccgattgc tcttttgtgt tttacagcct gtttttttgc
     3601 tgcagcttat tatctcgcta ttagaccaaa tggtatatta gatagtgttg gaaaaatctt
     3661 aacgcctgtt tttgcctttt taattctctc tttggttgtt gttggagcta ttgcttatgg
     3721 aaacttggag agtgctaagg cgagtgctga ctatgcagga aaagcgtttg gaagtggtgt
     3781 cttagcaggc tataatacac ttgatgcact tgcagcagtt gctttttgtc tagtagcaac
     3841 agaaacgctt aaaaagtttg gttttaaaac taaaaaagaa tatttatcaa ccatttggat
     3901 tgttggaatt gttactagtt tagcatttag tattttatat attggcttag gttttttagg
     3961 gaataagttt cctgtcccag ctgatatttt agctgaccca aatgttaata aaggagctta
     4021 tgtattatca caagcatctt ataaattatt tggaaatttt ggtcgttatt tcttgagtat
     4081 tatggtaaca ttgacatgct ttacaacaac agttgggctt attgtgtcag tatctgaatt
     4141 ttttgataag aactttcgtt ttgggaatta taaattattt gctactgttt ttactttgat
     4201 tggcttttta attgctaatc ttggtttaaa tgctgtaatt actttttcag ttcctgtgct
     4261 aactttattg tatccgattg ttattgttat tgtcttaatt attttgatta ataagtggtt
     4321 gccgctttca aaaaagggaa tgtctttgac aattggtctt gtcactttag tgtcgtttgt
     4381 tgaggttcta gcgggacaat ggcaagaaaa aacattaact cagttagttg gattcctccc
     4441 ctttcatact atttcaatgg ggtggctagt tccaatgttg attggtatag tattttcttt
     4501 ggtgctgtca gataagcaaa aaggccaagc atttgattta gagaaatttg aaggatagca
     4561 ctatttcaat tttatagata tgtcaaaaag gttaaaactt
//
LOCUS       DATRTN010000111         4535 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.111, whole genome shotgun sequence.
ACCESSION   DATRTN010000111 DATRTN010000000
VERSION     DATRTN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.111"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(25..95)
                     /locus_tag="VBE22_001821"
     tRNA            complement(25..95)
                     /locus_tag="VBE22_001821"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(61..63),aa:Cys,seq:gca)
     gene            complement(230..1762)
                     /gene="ahpF"
                     /locus_tag="VBE22_001822"
     CDS             complement(230..1762)
                     /gene="ahpF"
                     /locus_tag="VBE22_001822"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEO7203252.1"
                     /translation="MVLDKEIKAQLAQYLDLLESDIVLQADLGDNDNSQKVKDFLDEI
                     VAMSDRISLESTHLKRQPSFGIAKKGHESRVIFSGLPMGHEFTSFILALLQVSGRAPK
                     VDEDIIKRIKGIEKTINLETYVSLTCHNCPDVVQAFNIMAVLNPNITHTMIEGGMYQD
                     EVKSKGIMSVPTVYKDQEEFTSGRATIEQLLEQLDGPLDAEAFADKGVYDVLVIGGGP
                     AGNSAAIYAARKGLKTGILAETFGGQVIETVGIENMIGTLYTEGPKLMAQIEEHTKSY
                     DIDIIKSQLATGIEKKELVEVTLANGAILQAKTAILALGAKWRNINVPGEEEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGMEAALDLAGVTKHVTVLEFLPELKADQVLQERA
                     AKTDNLTILKNVATKDIVGEDHVTGLNYTDRDTNEEKHIDLEGVFVQIGLVPSTSWLK
                     DSGIELNERQEIVVDKFGSTNIPGIFAAGDCTDAAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(1780..2340)
                     /gene="ahpC"
                     /locus_tag="VBE22_001823"
     CDS             complement(1780..2340)
                     /gene="ahpC"
                     /locus_tag="VBE22_001823"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEO7203253.1"
                     /translation="MSLVGKEIIEFSAQAYHDEKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLDVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQGFDVLGQDGLAQRGTFIIDPDGVIQMMEINADGIGRDASTLIDKVRAAQYIRQH
                     TGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            2617..3387
                     /gene="rpsB"
                     /locus_tag="VBE22_001824"
     CDS             2617..3387
                     /gene="rpsB"
                     /locus_tag="VBE22_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEO7203254.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVAEEAKRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFELLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDVIIPANDDAIRAVKLITSKLA
                     DAVIEGRQGEDADVDFAQEAQADSIEEIVEVVEGSNND"
     gene            3481..4521
                     /locus_tag="VBE22_001825"
     CDS             3481..4521
                     /locus_tag="VBE22_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEO7203255.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDLDKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVIEVNAETDFVAKNDQFVTLVNETAKVIAE
                     GRPSNNEEALALTMPSGETLEQAFVTATATIGEKISFRRFALVEKTDEQHFGAYQHNG
                     GRIGVITVVEGGDDALAKQVSMHVAAMKPTVLSYTELDAQFVHDELAQLNHKIEQDNE
                     SRAMVNKPALPFLKYGSKAQLTDEVIAQAEEDIKAELAAEGKPEKIWDKIVPGKMDRF
                     MLDNTKVDQEYTLLAQVYIMDDSKTVEAYLESVNAKAVAFVRFEVGEGIEKASNDFEA
                     EVAATMAAALEK"
BASE COUNT         1457 a          860 c          855 g         1363 t
ORIGIN      
        1 tttggataaa atcttttata tttaaggcgg tagacggatt tgaaccgacg atcaagcttt
       61 tgcagagccg tgccttacca cttggctata ccgccataac agataatatt ctatcgaaaa
      121 aaaaaagatt cgtcaagcct ctttcgtaaa atagccaatt ttagccctta aacttcctca
      181 tattacccaa aaaaagagat tgatacccat tgtatcaatc tcttttagat tattgtctga
      241 ttaaataatc aaatgcgcca atggcagctg tggcaccaga ccccatggaa ataatgattt
      301 gcttgtaggc agcatctgta cagtcacctg cagcaaaaat accagggata tttgttgagc
      361 caaatttatc aacgacaatt tcttgacgtt cattgagttc aatgccgcta tccttgagcc
      421 aagatgtact tggtacaaga ccaatttgta caaaaacacc ttctaaatca atgtgttttt
      481 cttcgtttgt atcacgatcg gtatagttta aaccagtgac atgatcttca ccaacgatat
      541 ctttagtagc aacattctta agaatagtta aattgtcagt tttagctgct cgttcttgca
      601 gtacttggtc tgctttgagc tcaggtaaga attctaggac tgtaacatgt ttagtgacac
      661 cggctagatc taaagcagct tccatacctg aattaccacc acctataaca gcaacatctt
      721 ttccttcgaa aagagggcca tcacaatgtg gacagtaagt aacaccttta ttgcggaatt
      781 cttcctcgcc aggaacgttg atatttcgcc acttagcacc cagagctagg atagcagttt
      841 tagcttgtaa aatagctccg ttagccagtg ttacctcaac taattctttt ttctcaatgc
      901 cagttgctaa ttgactcttg ataatatcaa tatcgtaaga tttggtatgc tcttcaattt
      961 gagccattaa tttagggcct tctgtgtaca gtgtaccaat catgttctca ataccaactg
     1021 tttcgataac ttgtccacca aaagtttctg ctaaaatacc tgtttttaac cctttacggg
     1081 cggcataaat tgcagcactg ttacctgcag gaccaccacc aataactaga acatcataga
     1141 cacctttgtc agcaaatgct tcagcatcaa gaggtccatc tagttgttct aaaagctgtt
     1201 caattgtagc acggccagaa gtaaattctt cttggtcctt ataaacagta ggaacagaca
     1261 tgataccttt tgatttgact tcgtcttgat acatgcctcc ttcaatcata gtatgagtga
     1321 tattcggatt caaaacagcc attatattaa aggcttgaac gacatctgga caattatggc
     1381 aagttaatga aacataagtt tctagattga tagttttctc aatcccctta attcgtttga
     1441 taatatcttc atcaactttt ggtgcgcgac cagagacttg caacaaagct aaaatgaaag
     1501 atgtgaattc atgccccatt ggcaagcctg aaaaaataac gcgactttca tgcccttttt
     1561 ttgcaattcc aaaactaggt tgacgtttta aatgtgttga ttcaaggctg atgcgatcag
     1621 acatagcgac aatttcatct agaaaatcct ttactttttg agaattatcg ttatctccta
     1681 aatcagcttg taatacaata tcagattcta gcaaatctaa atattgcgct aattgagcct
     1741 tgatttcttt atctaagacc atggattacc tcctggttat taaattttac ctacaaggtc
     1801 taaactagga gttaaagttt cagctccttc tttccattta gctggacaaa cttcacctgt
     1861 atgttggcgg atgtattgag cagcacggac tttatcaatg agagtacttg catcacgtcc
     1921 gatgccatca gcattaattt ccatcatttg gatgacaccg tctggatcaa tgataaatgt
     1981 accacgttgt gctagaccat cttgtccaag gacatcaaaa ccttgtgaaa taaggtgtga
     2041 agggtcacca atcataggat atgtgatagt accaacgaca tctgagtcat catgccatgc
     2101 tttatgaaca aaatgagtat cagtagagac agaataaact tctacatcaa gagatttaag
     2161 tgtttcgtat tgctcttgaa ggtcaccaag ttcagttggg caaacaaatg aaaagtctgc
     2221 tgggtagaaa caaaaaactg cccattttcc tttaacgtct tcatttgtaa cagtgatgaa
     2281 tttttcatcg tgataagctt gagctgaaaa ttcaataatt tcttttccga ctaatgacat
     2341 aataatgtcc tcctttattt tttaatagcc taattataaa cgaaatactt ttaaatctcc
     2401 aataaaaacg tttacatttt tctaagaaaa gtatgatttt tctaatagag tagaagattt
     2461 tatagcttgt caaatctaac tttttttgat aaactattat agttgcttaa agtgacaaat
     2521 actcatctta gatcaattgt ggtcttgttg ccgaaaggtt aggctgcaag tcgaagtctg
     2581 agagaggaaa aaacattttt aaaggagaac ttactcatgg cagtaatttc aatgaaacaa
     2641 cttcttgagg ctggtgttca ctttggtcac caaactcgtc gctggaaccc taagatggct
     2701 aagtacatct tcacagaacg taatggtatc cacgttatcg accttcaaca aactgtaaaa
     2761 ttagctgacc aagcttacga atttgttcgt gatgctgcag caaacgatgc agttatcttg
     2821 tttgttggta ctaagaaaca agcagctgaa gctgttgctg aagaagctaa acgtgctggt
     2881 caatacttca ttaaccaccg ttggttaggt ggaactctta ctaactgggg aactatccaa
     2941 aaacgtattg ctcgtttgaa agaaatcaaa cgtatggaag aagaaggaac ttttgaactt
     3001 cttcctaaga aagaagttgc attgttaaac aaacaacgtg cacgtcttga aaaattcttg
     3061 ggtggtatcg aagatatgcc acgcatccca gatgtaatgt atgttgttga cccacataaa
     3121 gagcaaatcg ctgttaaaga agctaaaaaa cttggtattc cagttgtagc tatggttgac
     3181 actaacgctg atccagatga tatcgatgtt atcatcccag caaacgatga cgctattcgc
     3241 gccgttaaat taattacttc taaattagct gatgcagtta ttgaaggtcg tcaaggtgaa
     3301 gatgctgacg ttgacttcgc tcaagaagca caagcagatt caattgaaga aatcgttgaa
     3361 gtagttgaag gatcaaacaa cgactaatat ttaatttatt gtaaatccta aaacggggca
     3421 gatgcaaacc gctctgtctc gtttttttaa tcattatttg aaattaagag gagaacgaaa
     3481 atggcagaaa tcacagctaa acttgtaaaa gaattgcgtg aaaaatcagg tgcaggcgtt
     3541 atggacgcta aaaaagcatt agtagaaact gatggtgacc ttgataaagc gattgaatta
     3601 cttcgcgaaa aaggtatggc taaagcagct aaaaaagcag accgtgttgc tgctgaaggt
     3661 ttaacaggtg tttatgttga tggtaacgtt gcagcagtta ttgaagttaa cgctgagact
     3721 gactttgttg ctaaaaacga ccaatttgta acattagtta atgaaactgc taaagttatt
     3781 gcagaaggac gtccttcaaa caatgaagaa gcacttgctt taacaatgcc ttcaggtgaa
     3841 actttagaac aagcatttgt aactgcaact gctacaattg gtgaaaaaat ttcattccgt
     3901 cgcttcgctt tagttgaaaa gactgatgag caacattttg gtgcatacca acataatggt
     3961 ggacgtatcg gagtaattac tgttgttgaa ggtggcgacg atgcacttgc taaacaagta
     4021 tcaatgcacg ttgcagctat gaagccaact gttctttcat atactgaact tgatgcacaa
     4081 tttgttcatg atgaattagc acagttgaac cacaaaattg agcaagacaa cgagagtcgt
     4141 gctatggtta acaaaccagc tcttccattc cttaaatatg gatcaaaagc tcaattgact
     4201 gatgaagtta ttgcccaagc agaagaagac atcaaagcag aattagctgc agaaggtaaa
     4261 ccagaaaaaa tctgggataa aatcgttcca ggtaaaatgg atcgtttcat gcttgacaac
     4321 actaaagttg accaagagta cacactcctt gctcaagttt acatcatgga tgatagcaaa
     4381 acagttgaag cttatcttga gtcagtaaat gctaaagcag ttgctttcgt acgttttgaa
     4441 gttggagaag gtattgagaa agcttctaac gactttgaag cagaagttgc agctacaatg
     4501 gcagcagcac ttgaaaaata attaacactc gcttt
//
LOCUS       DATRTN010000112         4491 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.112, whole genome shotgun sequence.
ACCESSION   DATRTN010000112 DATRTN010000000
VERSION     DATRTN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.112"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            53..2500
                     /locus_tag="VBE22_001826"
     CDS             53..2500
                     /locus_tag="VBE22_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO7203256.1"
                     /translation="MVFMANKKKTKGKKTRRPTKVEIERQRAIQRMITALVLTIILFF
                     GIIRLGIFGITVYNVIRFMVGSLAYLFIAATLIYLYFFKWLRKKDGLVAGFLIASLGL
                     LIEWHAYLFSMPILKDKEILRSTARLIVSDLMQFKITVFAGGGMLGALIYKPIAFLFS
                     NIGAYMIGVLFIILGLFLMSSLEVYDIVEFIRAFKNKVAEKHEQNKKERFAKREMKKA
                     IAEQERIERQKAEEEAYLASVNVDPETGEILEDQAEDNLDDALPPEVSETSTPVFEPE
                     ILAYETSPQNDPLPVEPTIYLEDYDSPLLNMRENDEEMVYDLDDDVDDSDIENVDFTP
                     KTTLVYKLPTIDLFAPDKPKNQSKEKDLVRKNIRVLEETFRSFGIDVKVERAEIGPSV
                     TKYEIKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSF
                     RELWEQSDANPENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISS
                     ILMKARPDQVKFMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYE
                     LFSKIGVRNIAGYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIR
                     LGQKARAAGIHMILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKL
                     LGRGDMLFKPIDENHPVRLQGSFISDDDVERIVGFIKDQAEADYDDAFDPGEVSETDN
                     GSGGGGVPESDPLFEEAKGLVLETQKASASMIQRRLSVGFNRATRLMEELEAAGVIGP
                     AEGTKPRKVLMTPTPSE"
     gene            2582..4297
                     /gene="pabB"
                     /locus_tag="VBE22_001827"
     CDS             2582..4297
                     /gene="pabB"
                     /locus_tag="VBE22_001827"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEO7203257.1"
                     /translation="MHIETVIDFKELGKRYRFKNPTKELIADTLEQVLEVIKEVDYYQ
                     SKNYYVVGYLSYEASAAFDSHFKVSHQKLAGEHLAYFTVHKDCENEAFPLSYENVRLA
                     DNWTANVSEQEYQEAIANIKGQIRQGNTYQVNYTLELSQQLCLDPFSVYERLMVEQGA
                     GYNAYIAYDDKRILSVSPELFFKKKGEVLTTRPMKGTSARKPTYQEDVAERDWLANDP
                     KNRSENMMIVDLLRNDMGRICDVGTVKVKKLCQVEQYATVWQMTSTIEGVLSPEVTLT
                     SIFQALYPCGSITGAPKISTMAIINELEKRPRGIYCGTIGLCMPDGQAIFNVPIRTVQ
                     MKGQQAYYGVGGGITWESQTDSEYEETRQKSAVLTRVNPKFQLITTGRVTENKLLFSQ
                     QHVERLVESASYFAYPFDKSKFERELKKYLHQLDEKDYRLKIMLDKTGKVTFEVKQLV
                     NLSKKFLTAEVVVQDYPIKLSPFTYFKTSYRPHIIEGQNEKIFVSPEGLLLETSIGNI
                     VLEKNGRFLTPDLSEGGLNGIYRRHLLENQKVIEAPLTLKDLESADAVYACNAVRGLY
                     PLNLK"
     gene            complement(4346..>4491)
                     /locus_tag="VBE22_001828"
     CDS             complement(4346..>4491)
                     /locus_tag="VBE22_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7203258.1"
                     /translation="YGLEKSYKSLDDLEQAITDYIFYYNNKRIKAKLKGLSPVQYRTK
                     SFT"
BASE COUNT         1473 a          691 c          949 g         1378 t
ORIGIN      
        1 agtatagcat gagttgatag agaaagtgag gaacaagtat ttcacttgaa atatggtatt
       61 tatggcaaat aagaaaaaaa caaaaggaaa gaaaaccaga agacctacta aggtagaaat
      121 agagcgtcaa agagctattc aaaggatgat tactgctctt gttttaacaa ttattctctt
      181 ctttggtatt atcagattag gtatttttgg tattacagtc tacaatgtta tccgttttat
      241 ggtaggtagc ttggcttact tatttattgc ggcaacttta atctaccttt atttctttaa
      301 atggttgcga aagaaagatg gcttagtagc aggttttttg atagcttctt taggattatt
      361 gattgagtgg catgcttacc ttttctcgat gcctattttg aaagataaag aaattttgcg
      421 ttcaactgct cgattaattg tatctgattt aatgcaattt aaaatcactg tttttgccgg
      481 tggaggtatg ttgggtgctt tgatttacaa gccaattgct tttctctttt ctaatattgg
      541 tgcctatatg attggtgttc tcttcatcat tttgggtctc tttttaatga gttctctgga
      601 agtttatgac atcgtcgaat ttattagagc ttttaaaaat aaagtggcag agaagcacga
      661 gcaaaataaa aaggagcgtt ttgctaagcg agagatgaaa aaagcaatcg ctgaacaaga
      721 gcgcatagag cgtcaaaaag ctgaagaaga agcttattta gcttcggtta atgtagaccc
      781 tgaaacgggt gagattctag aggatcaagc tgaggacaat ttggatgatg cgctaccacc
      841 tgaggtaagt gaaacatcaa ctccggtatt tgagccagag atccttgctt atgagacatc
      901 gcctcaaaat gatcctttac cagtagagcc gacaatttat ttagaagact atgattcgcc
      961 gcttcttaat atgagagaaa atgacgagga aatggtttat gatttagatg atgatgtaga
     1021 tgatagtgat atagaaaatg tcgactttac acctaaaacg acactggttt ataaattacc
     1081 aacgatagat ttatttgcac cagataagcc taaaaatcaa tccaaagaaa aggatttagt
     1141 ccgaaagaat atcagagttt tagaagaaac atttagaagt tttggtatcg atgtaaaagt
     1201 agaacgtgct gaaattggac catcggttac taaatatgaa attaaaccag cagttggagt
     1261 tcgtgtgaat cgtatttcaa atctatctga cgacctagct cttgctcttg cggcaaaaga
     1321 tgttcgtata gaagcaccaa ttcctggaaa atcattaata ggtattgaag ttcctaactc
     1381 agaaattgca acggtttctt tccgcgaact ttgggaacaa tctgatgcca atcctgaaaa
     1441 ccttttagaa gtaccactag gaaaagctgt taacggcaat gctcgcagtt ttaacttagc
     1501 tagaatgccg catcttttgg tagctggttc aactggttca ggtaaatctg tggcagttaa
     1561 tggaattatt tcaagtattt tgatgaaggc acgtccagat caagttaagt ttatgatgat
     1621 tgaccccaaa atggttgaat tatctgttta taatgatatt ccacatttat taatccctgt
     1681 tgtaaccaat ccgcgtaaag caagcaaggc actccaaaaa gttgttgatg aaatggaaaa
     1741 tcgatacgag ttatttagca aaattggtgt gcgtaatata gcaggctata atacaaaggt
     1801 tgaagagttt aatgcttcct ctgagcaaaa acaaatacct ttgcctttaa tcgttgtcat
     1861 tgtagatgaa ttagctgact tgatgatggt tgctagtaaa gaagttgaag atgctattat
     1921 tcgtttgggg caaaaagcac gtgctgcagg tatccatatg attcttgcaa ctcaacgtcc
     1981 atccgtagat gttatttctg gtttgattaa agcaaatgtt ccgtcgcgta ttgcatttgc
     2041 tgtttcaagt ggtactgata gccgtacgat ccttgatgaa aatggtgctg aaaagctctt
     2101 gggacggggt gacatgctct ttaagcctat tgatgagaat catccagtac gactacaagg
     2161 ttcctttatt tcagatgatg atgttgaaag gatcgttggt tttatcaaag accaagccga
     2221 ggctgactat gatgatgcct ttgatcctgg agaagtatct gaaacagata acggctctgg
     2281 tggtggcgga gtacctgaaa gtgatcctct ttttgaagaa gccaagggac tcgttttaga
     2341 gacgcaaaaa gcaagtgcct caatgattca acgccgattg tctgttggtt tcaatagagc
     2401 aacaagacta atggaagagt tagaagcagc gggggttatt ggtccagcag aaggaaccaa
     2461 gccacgaaaa gttttaatga ctccaactcc gagtgaataa gaaaaaacta atctgacaag
     2521 aaattgtcgg attagttttt tatgttataa tgaagcaaat agatgaagaa gaaggttacg
     2581 aatgcatatt gagactgtta ttgatttcaa agaattagga aaaagatatc gttttaaaaa
     2641 tcctacaaaa gaattaatag ctgatacttt agaacaagtc ttagaagtga taaaagaagt
     2701 tgattattat caatctaaga attattatgt tgttggttat ttatcttatg aagcatctgc
     2761 tgcttttgat tcacatttta aagtttctca tcagaagttg gctggagaac atctagctta
     2821 ttttacagta cataaagatt gtgagaacga agcttttcct ttaagttatg aaaatgttag
     2881 attagcagat aattggactg ctaatgtttc tgagcaagaa tatcaagagg caattgccaa
     2941 tattaaagga caaattagac aaggaaatac ttatcaagta aattatacac tagagcttag
     3001 ccaacaatta tgcttggatc cctttagcgt ttatgaacgc ctaatggtag aacaaggagc
     3061 aggatataat gcttatattg cctacgatga taaaaggatt ttatctgtaa gcccggaatt
     3121 gttctttaag aagaagggtg aagtcttaac aactagacca atgaagggaa caagcgctag
     3181 aaagcctact tatcaagaag atgtggcgga gcgagattgg ttggcaaatg atcccaaaaa
     3241 tcgttctgaa aatatgatga ttgttgattt attaagaaac gatatgggcc gtatctgtga
     3301 tgttggaaca gttaaggtaa aaaaactatg tcaagtggag cagtatgcaa ctgtgtggca
     3361 aatgacatca actattgaag gagttttatc gccagaagtg acacttacgt ctatttttca
     3421 agctctatat ccttgtggat ctatcactgg agctcctaag atatcaacaa tggctattat
     3481 taatgagttg gaaaaacgcc caagaggtat ttattgtggg acgataggac tttgtatgcc
     3541 agacggacaa gctattttta acgttcctat tcgcacagta caaatgaaag gtcaacaagc
     3601 ctattacggc gttggtggag gtattacgtg ggagagccag acagattctg aatatgaaga
     3661 aacccgtcaa aaatcagctg ttttaacacg tgtcaatcca aaatttcaat taataactac
     3721 aggaagagtg actgaaaata aacttctttt ctctcaacaa catgtcgaga gattagttga
     3781 atcagcgtct tattttgctt atccttttga taaaagtaaa ttcgaaaggg aactgaaaaa
     3841 ataccttcat cagctagatg agaaggatta ccgcttgaaa ataatgcttg ataaaactgg
     3901 taaggtaacg tttgaggtaa aacaactagt gaatttatca aaaaagtttt taacggcaga
     3961 ggtggtcgta caagactacc ctattaaatt atccccgttt acttatttta aaacttctta
     4021 tcgcccacat attattgaag gtcagaatga aaagatattt gtatctcctg aggggttgct
     4081 attggaaaca agtattggga atattgtttt agaaaaaaat ggaaggtttt taaccccaga
     4141 tttatcagaa ggagggttga atgggattta tcgtcgtcat ctccttgaaa accaaaaagt
     4201 aattgaagca ccactaactt taaaagattt agaatcagcc gatgctgtat acgcctgtaa
     4261 tgctgttaga gggctttatc ctctaaacct aaagtaaaaa gagttggaaa ttttgtactg
     4321 acccccaaaa gttggacaat ttattttaag tgaaggattt agttctgtat tgcacaggac
     4381 taagtccttt tagttttgct ttgattcgct tgttgttgta gtaaaaaatg taatctgtga
     4441 tagcttgctc aaggtcatca agtgatttgt aagacttctc caagccatag a
//
LOCUS       DATRTN010000113         4482 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.113, whole genome shotgun sequence.
ACCESSION   DATRTN010000113 DATRTN010000000
VERSION     DATRTN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.113"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            54..1070
                     /locus_tag="VBE22_001829"
     CDS             54..1070
                     /locus_tag="VBE22_001829"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO7203259.1"
                     /translation="MTKQKIAVLGPGSWGTALAQVLNDNGHEVRLWGNVVEQIEEINT
                     SHTNQRYFKDITLDSKIKAYTNLEEAINNVDSILFVVPTKVTRLVAKQVANLLKHKVV
                     IMHASKGLEPGTHERLSTILEEEISEQYRSDIVVVSGPSHAEEAIVRDITLITAASKD
                     IEAAKYVQKLFSNHYFRLYTNTDVVGVETAGALKNIIAVGAGSLHGLGYGDNAKAAII
                     TRGLAEITRLGVQLGADPLTFSGLSGVGDLIVTGTSVHSRNWRAGDALGRGEKLEDIE
                     KNMGMVIEGISTTKVAYEIAQNLNVYMPITEAIYKSIYEGANIKDSILDMMSNEFRSE
                     NEWH"
     gene            1107..2006
                     /gene="galU"
                     /locus_tag="VBE22_001830"
     CDS             1107..2006
                     /gene="galU"
                     /locus_tag="VBE22_001830"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEO7203260.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNELLKLVDETTGIRLHFIRQ
                     SHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNNKVIPLTKQLINDFEATHASTIA
                     VMEVPHEDVSAYGVIAPQGEGVNGLYSVNTFVEKPSPEEAPSNLAIIGRYLLTPEIFN
                     ILETQKPGAGNEIQLTDAIDTLNKTQRVFARKFTGDRYDVGDKFGFMKTSIDYALQHP
                     QVKDDLKKYIIDLGKSLEKTSKK"
     gene            complement(2097..3140)
                     /locus_tag="VBE22_001831"
     CDS             complement(2097..3140)
                     /locus_tag="VBE22_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO7203261.1"
                     /translation="MNRKIIGLTLLSLSVLTLTACGNRSDKSANKSDIKVAMVTNQGG
                     VDDKSFNQSAWEGLQKWGKKKGLTKGNGFDYFQSSNESDHANNLDTAASSGYNLIFGI
                     GFGLHDTIEKVSENNKDVKYVIVDDIIKGKENVASVTFADNEAAYLAGIAAAKTTKTK
                     TVGFIGGMEGVVVKRFEAGFKAGVKSIDPAIKVAVSYAGSFTDAAKGKTIAATQYATG
                     VDVIYQAAGGTGAGIFSEAKTENETRKESNKVWVIGVDRDQSQEGNYVSKDGKKANFV
                     LASTIKEVGKSLQSVAELTEKKQYPGGKVTVFGLKDSGVDIKEHQLSSEGSVAVKKAK
                     EDIVSGKIQVPMK"
     gene            complement(3272..3949)
                     /locus_tag="VBE22_001832"
     CDS             complement(3272..3949)
                     /locus_tag="VBE22_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEO7203262.1"
                     /translation="MSMKKFAKEYPTTVLLVGLTTLVFLLMQLTYGSQAESSQVIFQF
                     GGIQGDYLKAYPTNLWRLISPIFVHIGWEHFLLNGLALYFVGQMGESIWGSLRFLILY
                     ILSGLMGNIFTLFFTPHVVAAGASTSLFGVFSAIAIAGYFGKNPYLKQVGKSYQVMIL
                     LNLFFNIFTPGVSLAGHVGGLVGGVLVAIFLTKQNGSLLFKTWQSILALMIFIIVSIS
                     LIGLSLV"
     gene            complement(3940..4467)
                     /locus_tag="VBE22_001833"
     CDS             complement(3940..4467)
                     /locus_tag="VBE22_001833"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEO7203263.1"
                     /translation="MEKKLLRKEVLITLKSQPQAYKSEVDSKLLEAFIKTKAYQNSCV
                     IATYLSFDYEYNTQLLIKQALCDGKRVLVPKTYPKGKMIFVDYQKDNLRATLFGLLEP
                     VNDRAVEKASIDLIHVPGLIFNNKGFRIGYGAGYFDRYLSDFEGDTISTIYRCQRQDF
                     VEEKHDVAVKEVLCL"
BASE COUNT         1483 a          846 c          723 g         1430 t
ORIGIN      
        1 agtatgatat aatgttagcg ctatatgcct tattattaga aagaaggaat aagatgacaa
       61 aacaaaaaat tgccgttctt ggacctggtt catgggggac tgctcttgca caagtattaa
      121 atgacaatgg acatgaggta cgcttatggg ggaacgtggt tgaacaaata gaagagatta
      181 atactagcca tactaatcaa cgttacttca aagatattac cttagattcc aaaataaaag
      241 cctacactaa tcttgaggaa gcgattaata atgttgacag cattctattc gtcgtcccaa
      301 ctaaagttac aagacttgtt gctaaacaag tggctaatct tttaaagcat aaagttgtta
      361 ttatgcatgc ttctaaggga ctagaacctg gcactcatga acgactttct acaattttag
      421 aagaagaaat ctctgaacaa tatcgtagtg atatcgttgt tgtttcaggt cccagtcacg
      481 ctgaagaagc catcgttcgt gatattactt tgattactgc tgcttcaaaa gatattgaag
      541 cagctaagta tgtccaaaaa ttgtttagta atcactattt tagactttat actaacactg
      601 atgtcgtagg agtggaaaca gctggtgctc taaaaaatat tattgctgtt ggtgctggat
      661 cactacacgg tcttggttat ggtgataatg ctaaagcagc tattattacg cgtggtttag
      721 ctgagataac acgccttggt gtacaattag gagccgaccc tctcactttc agtggtttat
      781 ccggagttgg agatttaatt gtgactggta catctgttca ttctcgcaac tggagagcgg
      841 gagatgcttt aggacgtggt gagaaacttg aagatattga aaaaaatatg gggatggtta
      901 ttgaaggtat ctcaacaact aaggttgctt acgaaatcgc ccaaaactta aatgtttaca
      961 tgcccattac agaagctatt tataaatcaa tatatgaagg ggctaatatc aaagatagca
     1021 ttttagatat gatgtccaat gaatttcgtt cagagaatga atggcactaa tatcgtttat
     1081 attaatacta tttaaaggag ctatatatga aagtcagaaa agcagttatc cctgccgctg
     1141 gtttaggaac acgtttttta cctgctacta aagctttagc taaagaaatg cttccaatcg
     1201 ttgataaacc aactattcaa tttattgttg aagaagcact aaaatctgga attgaagata
     1261 ttttagttgt aactggtaaa tcaaaacgtt ctatagaaga ccactttgat tcaaattttg
     1321 aattagaata taacctaaaa gagaaaggga aaaacgaact tctcaaactt gttgatgaaa
     1381 caactggcat tcgactccat tttattcgac aaagtcatcc tcgtgggctt ggagatgctg
     1441 tgctacaagc aaaagccttc gttggtaatg aaccttttgt tgttatgcta ggtgatgatc
     1501 taatggatat taccaataat aaagttattc ctctcaccaa acaattaatt aatgactttg
     1561 aagctactca tgcatcaaca attgctgtta tggaagttcc acatgaagat gtttctgctt
     1621 atggtgttat cgctccacaa ggagaaggag ttaatggtct atatagcgtt aacactttcg
     1681 ttgaaaaacc ttctcccgaa gaggctccaa gtaatttggc aatcattggt cgctacttgt
     1741 taactccaga aatttttaat attttagaaa ctcaaaaacc tggtgcagga aatgagattc
     1801 aactaaccga tgctatcgat actttaaata aaacgcaacg tgtttttgct agaaaattta
     1861 caggggaccg ttatgacgtt ggtgacaagt ttggttttat gaaaacatct attgattatg
     1921 ctcttcaaca cccacaagtt aaagatgact tgaagaaata tatcattgat ttaggaaaat
     1981 ctctagaaaa aacatctaag aagtaaaata tgagtcaagt acaataaaaa agtttcctaa
     2041 ataggaaact tttttattgt acttgacgaa gaacttattg ttcctccaca cttctatcat
     2101 ttcataggaa cttgtatttt ccctgaaaca atatcctctt tagctttttt gacagcaaca
     2161 gatccctcac tagacaattg atgctcttta atatctacac cactatcctt tagaccaaaa
     2221 actgttactt tccctcctgg atattgtttt ttctctgtta attctgctac agattggaga
     2281 gatttcccca cctcttttat agtagatgcc agaacaaagt ttgctttctt accatctttt
     2341 gaaacataat tgccttcttg actttgatct ctgtcaacac caattaccca gactttgtta
     2401 gattccttgc gggtctcatt ttcagtttta gcctcactaa aaattcctgc tcctgtacca
     2461 ccagcagcct ggtaaataac atcaacacct gttgcatact gtgtggcagc aatcgtttta
     2521 cctttagctg catctgtaaa tgagcctgca taagataccg caactttaat agccggatca
     2581 attgatttta ctccagcctt aaatcctgct tcaaatcgct taacaacgac gccttccatc
     2641 cctccgataa aaccaactgt ttttgtctta gttgtcttag ctgcagcaat tccagctaag
     2701 taagcagctt cattgtctgc aaaagtaaca ctagctacat tttctttacc tttaatgata
     2761 tcatctacaa tgacgtattt aacatcttta ttattttctg atactttttc aatagtatca
     2821 tgaagaccaa atccaatacc aaaaattaaa ttatagccac tagaggctgc cgtatcaagg
     2881 ttattagcat gatctgattc attagatgac tggaaataat caaagccatt accttttgtc
     2941 aaccccttct tttttcccca cttttgtagc ccttcccaag ctgattgatt aaacgattta
     3001 tcgtctactc caccttgatt agttaccatc gcaactttga tatctgattt atttgcagac
     3061 ttatctgaac gattaccaca tgctgttaat gttagtacag ataaagatag tagagttaag
     3121 ccaataattt ttctgttcac cgagaatgaa cctcctaata agttatagtc acataaaaac
     3181 agatttctat cataacataa aaaaatcatt tagcaaactt tattctattt ttcccagaga
     3241 aagtttggtt ttagagacta tagccgtaga attataccaa cgatagacct attaagctga
     3301 tcgatacaat aataaatatc attaaagcta aaatactttg ccaagtttta aataataaag
     3361 aaccgttttg ttttgtcaaa aaaatagcca ccaatactcc accaactaaa ccaccaacat
     3421 ggcctgctaa actcactccg ggtgtgaaga tattaaaaaa taaatttaac aagatcatca
     3481 cttgatatga ttttccaact tgttttaaat aaggattttt tccaaaataa cctgcgattg
     3541 ctatagcaga aaaaacacca aataaagacg ttgaggctcc tgctgccacc acgtgtggag
     3601 taaaaaatag cgtaaaaata ttacccatta agccagataa aatatagaga attagaaatc
     3661 tcaaacttcc ccaaatactt tcccccattt gtcctacgaa atatagagct aaaccattta
     3721 ataaaaaatg ttcccaacca atatgaacaa agataggact tattagtctc catagatttg
     3781 ttggatatgc ttttagataa tcaccttgta ttccaccaaa ttgaaaaata acttggctag
     3841 actctgcttg acttccataa gttagttgca tcaataaaaa aacaagggtt gtcagaccga
     3901 ccaagagtac tgtcgtcgga tactctttag cgaacttctt catagacata atacctcctt
     3961 aaccgctaca tcatgcttct cttctacaaa atcctgtctc tgacacctat aaatagtact
     4021 aatagtatca ccttcaaaat cactcaaata gcgatcaaaa taacctgcac cataacctat
     4081 acgaaaacct ttattgttaa agataagtcc gggcacatga attaagtcta tactagcttt
     4141 ttcaacagct ctatcattaa caggttctaa taagccaaac agtgttgccc tcaagttatc
     4201 tttttgatag tccacaaaaa tcattttacc ttttggataa gttttgggaa caaggacacg
     4261 tttgccatca cataaagctt gtttaataag aagttgtgta ttgtattcgt agtcaaaaga
     4321 taggtaagta gcaattacac aactattttg ataagcttta gtttttataa aagcctctaa
     4381 aagtttagag tcaacttctg atttgtaagc ttgaggttga gattttaagg ttatcaaaac
     4441 ttcttttctt aataatttct tttccatata tctatgatac ca
//
LOCUS       DATRTN010000114         4436 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.114, whole genome shotgun sequence.
ACCESSION   DATRTN010000114 DATRTN010000000
VERSION     DATRTN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.114"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..525)
                     /locus_tag="VBE22_001834"
     CDS             complement(<1..525)
                     /locus_tag="VBE22_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEO7203264.1"
                     /translation="MNRIWRRYKPFISTGIQGLITYRVDFILYRIGDVIGAFVAFYLW
                     KAVFDSSSQSLIQGFQLSDMILYIIMSFVTNLLTRTDSSFMIGDEVKDGSIIMRLLRP
                     VHFAASYLFMEIGSRWLIFLSIGVPFLLVITGVRLFLGTDLIQAIVLVVFYIISIILA
                     FLINFFFNICFGFSA"
     gene            complement(528..1520)
                     /locus_tag="VBE22_001835"
     CDS             complement(528..1520)
                     /locus_tag="VBE22_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7203265.1"
                     /translation="MSMIEVSHLQKNFIKTVKAPGLKGAFQSFLRPEKHTFEAVKDLT
                     FDVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRIDGKLPQENRQNYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDKEFRKRMAFLNEVLELNDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCHRIFMIDRGQEIFDGTVSQLKETFGKMKTLSFDLRPGQEHISSSLIGKSEIN
                     IKRNDLVLDIQYDSSRYQTADIIQQTLADFSVRDLKMTDADIEDIIRRFYRKEL"
     gene            complement(1720..2871)
                     /locus_tag="VBE22_001836"
     CDS             complement(1720..2871)
                     /locus_tag="VBE22_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO7203266.1"
                     /translation="MVRKFLKSLLSLFLIAVIATGISVACFFFIPENKGNITPILLHR
                     FMRKNNVNGMMIVSDNTGKPITISHGINRGEVETDIENNKLFPMASLQKLMTGIIIQR
                     LIDQDVLSEDDRLSQFFPQVKGSNSITIHQLLTHTSGLREKGVKVSPYLKNEREQLQF
                     CLKHYNFVNKKSWYYSNINFSFLTGIATQVTGRTYAELVDDVIKNPLRLDDIQSYQSV
                     VNHDLVSPMRKNGKLNKINIFNQVSTAYGAGDFFTTPLNFWVLMRSFSKGYFFPTDEY
                     TKHQNDAISHYYGGLYMHGRIVNSNGAFFQYSSFGYADLKTHDTMVLFMNNKVYSDAS
                     HVAPKAFEYYQKFMFLNKIFHLVFYFVFSFFLMLLIRHSFRKRRYKKRH"
     gene            complement(2932..3201)
                     /locus_tag="VBE22_001837"
     CDS             complement(2932..3201)
                     /locus_tag="VBE22_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203267.1"
                     /translation="MVSLLLLKVKQEELIEFHNVFYREAISSYLETENFEKAKVTEEN
                     YQVFLGIRTYQKSYNYQDHSENLKGNIEVAMIGVENNISDEHIFC"
BASE COUNT         1503 a          828 c          640 g         1465 t
ORIGIN      
        1 tgccgagaag ccaaaacaaa tattaaaaaa gaagttaatc aaaaaagcta aaataatgct
       61 aataatataa aataccacta acactatagc ttgaatcaaa tcagtcccca agaagaggcg
      121 tacccccgta ataacaagca aaaacggtac ccctatactt aaaaagatga gccatctaga
      181 accaatctcc atgaaaaggt aggaagcagc aaaatgaact ggacgtaaga gtcgcataat
      241 aattgaaccg tcttttactt cgtcacctat cataaatgaa ctatcagttc ttgttaataa
      301 attagtcaca aaactcataa taatatagag aatcatatct gacagctgaa aaccttggat
      361 taaggattga cttgaggaat cgaaaactgc tttccataga taaaatgcaa caaatgctcc
      421 tatcacatca cctatcctat aaaggatgaa atcaacacga tatgtgatta atccttggat
      481 tcctgtacta ataaaaggtt tataacgtct ccaaattctg ttcatcctta tagctccttt
      541 ctatagaagc gacgaatgat atcttcaata tccgcatctg tcattttaag gtcacgtaca
      601 gaaaaatctg ctaatgtctg ctgaattatg tcagcagttt ggtagcgaga actatcatac
      661 tgaatatcta aaactaagtc attacgttta atattaatct ccgactttcc aattaaacta
      721 gagctgatat gctcttgtcc tgggcgcaaa tcaaaagaaa gagttttcat ctttccaaat
      781 gtctctttta gctgagaaac tgtgccatca aatatttctt gtcctctatc aatcataaaa
      841 atacgatgac aaagttgttc aatatcactg aggtcatgtg tcgttagtaa aatcgttgtc
      901 tcttcttctt gattaatttg tgtgattgca cgtcggatat tatctttcac tgaaacgtcc
      961 aagccaatgg tgggttcatc taagaaaaga acctttgggt tatgtaataa agaagctgcg
     1021 atatccgctc tcatacgctg tccgagagac aaagttctaa caggatcctt tataaagtca
     1081 tttagttcca aaacctcatt taaaaaagcc atgcgtttgc gaaattcttt atcaggaacg
     1141 tcatagattt cttttaacac tgtataggtt tcttgtagag ctaaatccca ccacaactgc
     1201 gtacgttgcc caaaaacgac tccaatatcc ttaacataat tttgtcggtt ttcttgtggc
     1261 aatttgccat caatacggca aaagcccgaa gttggtttta aaatcccagt taacatttta
     1321 attgttgtag attttcctgc accgttcgct ccaataaatc ccaaaatttg tcctttaggc
     1381 acatcaaatg ttaaatcttt aacagcttca aaggtgtgtt tttcaggacg aagaaatgat
     1441 tgaaatgctc cttttaaccc tggtgcttta acggttttta taaaattttt ctgaaggtga
     1501 gaaacttcaa tcattgacat attaatacct cttttactca acagtatttt tatagcttaa
     1561 atcagctcga cagttagatt attaaactat aataatgatt aacagctatt tattatagca
     1621 caattaaaaa ttcaatgcaa acgttttctt agatggtgtt actgcttaat attgaataaa
     1681 aaagaaggcc aacttagcct tcttttttat tcatgtgatt tagtgacgtt tcttatacct
     1741 tcttttccta aacgaatgac gtattaaaag cattaaaaag aaagaaaata caaagtaaaa
     1801 gactaagtgg aaaatcttat taaggaacat aaatttttga taatattcaa atgcttttgg
     1861 tgccacgtga ctagcatcac tatagacttt gttattcata aaaagcacca ttgtatcatg
     1921 cgtctttaga tctgcataac caaaacttga atactggaaa aaggccccat tagaattaac
     1981 tatacgacca tgcatgtaaa gcccaccata ataatgtgat atcgcatcat tttgatgttt
     2041 ggtatattca tccgttggga aaaagtaccc tttagaaaaa gaccgcatca acacccaaaa
     2101 gttcagcgga gttgtgaaaa aatctccagc tccatatgct gtggatactt gattaaagat
     2161 attaatttta tttaacttgc cgttctttcg cattgggcta actaagtcat gattaacaac
     2221 tgattggtaa ctttggatat catctaatcg caaaggattt tttatcacat catcaacaag
     2281 ttcagcgtag gtccttcccg taacttgagt ggctatacct gttaggaaac taaagttgat
     2341 attagaataa taccacgatt tcttattaac aaagttatag tgttttaagc aaaattgtaa
     2401 ttgttctctc tcatttttta gataaggaga caccttaaca cctttttctc ttaacccact
     2461 cgtatgggtc aacaattggt ggatggtaat actattacta ccttttactt ggggaaagaa
     2521 ctgagacaat cggtcgtctt cactcaagac atcttgatca attaatcttt ggataataat
     2581 tcctgtcatg agtttttgga gagaagccat tggaaataat ttattatttt caatatccgt
     2641 ttcaacttcc ccacggttaa tcccatgcga gatggtaatc ggtttaccag tattatcgct
     2701 aacaatcatc attccattaa cattattttt tcgcataaag cgatgcaata aaatcggtgt
     2761 aatattccct ttattttctg gaataaaaaa gaaacatgcc actgaaatac cggtggcgat
     2821 tactgctata agaaagagag ataggagcga ttttaaaaat ttacgtacca taaataacct
     2881 ccttaatatc ataacacagg gctaaattat ttcaaagaca ttttatatat tctagcaaaa
     2941 aatatgttcg tcgctaatat tattttcaac tcctatcatt gctacctcaa tatttccttt
     3001 tagattctca ctatgatctt gatagttata actcttctga taagtacgga ttcctaaaaa
     3061 aacttggtaa ttctcttcag tcactttagc cttctcaaaa ttttctgttt caagataaga
     3121 agatatcgct tctctataaa aaacattatg aaactctatc aactcctcct gctttacttt
     3181 caagagaagc aagctcacca tttctttacc ctgtttaaaa cactttctct agtgtcactc
     3241 caatctttta aactgttttt aggacatttt ttaaagacag agataaggtt atcaatgtcc
     3301 ttgaatcgcc ttattcaaac accaaacttg agcaaccaat aatctcatca aaatcatcaa
     3361 gcacaaacat ttagctttag aaactttgaa tattttaaaa agagaattca tatcactttg
     3421 aatatcaaaa aggagaaaac caatttggtt ttctccagaa tataattttt catcaaccca
     3481 ctacagttga caaagagcca aaaaacaagg aatcaatcaa gaaattttga ttgattcctc
     3541 gttttatgct tatatgagat actttaaaca aattttttca atgagatcca actatctagt
     3601 gactcctcga tatcctttta tatctctcac gatagctttg aggagtaagt actattccat
     3661 tcgttttacc tataatttca ccaaatgcat acataccata tcctatataa cggctatcgt
     3721 tttaagaaca atttatttat gttggcagaa aataataata ggaagcagaa aaatccaata
     3781 atattcaaga gaaaaagtat aggtgatagc tctctaaaaa aatttgatat tgaaactata
     3841 agaaaccctg tagatacgat agttgaaaca acctttttct tatccatatt aataaccacc
     3901 ccttaaaaca atttcatatc ctatatactt tgtacgcata acgaatccct cctaaattat
     3961 ttttttgtct gttgcaaccc tgacaagtta gtgtagcacg aaaaaacgat tatattctca
     4021 aaatcttatc ttctagctgc tcaacttctt cttcaatacg agggtcttca tcattcttag
     4081 tgttaaataa agcgagattg gatcaaccag tgtcacgtta gcctctccaa gctatttatt
     4141 tttctagtaa tcaaagggat tggttcataa ttccaaatct ctagccgatg actttgaagg
     4201 atgtatagtt gaagacttga ttcgaaactt ttgtatcttt tccttgaaat agtatagtct
     4261 ccgtattttt ccgaatcatc aagcaagcct agatatgata agtctttaaa ataagtcggt
     4321 taaaataata gctcttgttt taatttttga aaatccttat cacttataat caacgcttct
     4381 ctaactggac tatctattag atctttggac tcttcaaata tcatattttt tggctt
//
LOCUS       DATRTN010000115         4355 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.115, whole genome shotgun sequence.
ACCESSION   DATRTN010000115 DATRTN010000000
VERSION     DATRTN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.115"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..224
                     /locus_tag="VBE22_001838"
     CDS             <1..224
                     /locus_tag="VBE22_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEO7203268.1"
                     /translation="ATITVFSKNNCMQCKMTKKFLDQHGADFEEINIDEKPEKIEYVK
                     NLGFSAAPVIEAGNVVFSGFQPSKLKELV"
     gene            302..2461
                     /gene="nrdE"
                     /locus_tag="VBE22_001839"
     CDS             302..2461
                     /gene="nrdE"
                     /locus_tag="VBE22_001839"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO7203269.1"
                     /translation="MSLKNIGDVSYFRLNNEINRPVNGQIPLHKDKEALTAFFKENVQ
                     PNSKAFDSITDKIAYLLKYDYLEEAFLNKYRPEFIEELSTKLFDKKFRFKSFMAAYKF
                     YQQYALKTNDGEYYLESIEDRVLFNALYFADGDEKLATDLALEMISQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNAIGRSINSALQLSRIGGGVGISLSNLRDAGAPIKGF
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARNNQEMYLFSPYSIEREYGVPFSYIDITEKYDELVANPNI
                     TKTKINARDLETEISKLQQESGYPYIINIDTANRTNPIDGKIIMSNLCSEILQVQKPS
                     LINDAQEYLEMGTDISCNLGSTNVLNMMTSPDFGKSIKTMTRALTFVTDSSNIEAVPT
                     IKNGNAQAHTFGLGAMGLHSYLAKNHIEYGSPESIEFTDIYFMLMNYWTLVESNNIAR
                     ERQTTFVGFEKSKYADGTYFDKYVSGKFVPQSDKVKSLFANHFIPEAKDWENLRYAVM
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLATDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSMTLFLRSELPKELYEWKTESKQTTRDLS
                     ILRNYAFNKGVKSIYYIRTFTDDGSEVGANQCESCVI"
     gene            2664..3623
                     /gene="nrdF"
                     /locus_tag="VBE22_001840"
     CDS             2664..3623
                     /gene="nrdF"
                     /locus_tag="VBE22_001840"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO7203270.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSAQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEEIFEWTNNNEFLQEKARIINDIYANGNALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMHDDDYNYGL"
     regulatory      3708..3806
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3864..>4355
                     /gene="thiT"
                     /locus_tag="VBE22_001841"
     CDS             3864..>4355
                     /gene="thiT"
                     /locus_tag="VBE22_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEO7203271.1"
                     /translation="MSKNNNTTCLIETAIFAALAMALSMIPDFASWFTPSFGAIPLIL
                     FALRRGTKYGLFAGLIWGLLHFVLSKVYYLSLSQVFIEYILAFISMGLAGVFSAKFKD
                     ALSSSSKTKALSLALSGAILATFVRYVWHYIAGVIFWASYAPKGMSATLYSLSVNGTA
                     GLLT"
BASE COUNT         1430 a          780 c          738 g         1407 t
ORIGIN      
        1 tggcaactat tactgttttt tcgaaaaaca actgtatgca atgtaaaatg actaagaaat
       61 ttcttgacca gcacggtgct gattttgaag aaattaatat tgatgaaaaa ccagaaaaaa
      121 ttgaatatgt gaaaaatctc ggcttttctg cggctccagt gattgaagct ggtaatgttg
      181 ttttttcagg tttccaacca agcaaactaa aagaacttgt ttaagcaaat tagagtctgg
      241 gaataaagca attaattgct ttattcccat tttgtattgt taagttaaga ggataaattc
      301 aatgagttta aaaaatattg gcgatgtatc ttactttcgc ctaaataatg aaattaaccg
      361 tcctgttaat ggacaaatcc cactccataa agataaagaa gccttaactg cttttttcaa
      421 agaaaacgta cagcctaatt caaaagcttt tgactcaatc actgataaaa ttgcttacct
      481 ccttaagtat gattatcttg aagaagcttt cctcaataaa tatcgtcctg aatttattga
      541 agaattatct accaaactct tcgataaaaa gtttcgtttt aaatccttca tggcagccta
      601 taagttttat cagcagtacg cattaaaaac aaatgatgga gaatactatt tagaaagtat
      661 tgaagatcgc gttctcttta acgctcttta ttttgcagac ggtgatgaaa aactagcaac
      721 tgacttagct ctagaaatga tttcacaacg ctatcaacct gcaacaccaa gttttttaaa
      781 tgctggtcga agtcgtcgcg gagaattagt ctcatgtttc ttaatccaag taactgatga
      841 tatgaatgct attggtcgtt caattaattc agctcttcaa ttatctcgta ttggtggtgg
      901 ggttggaata tcacttagca accttcgtga tgctggagct cctatcaaag gttttgctgg
      961 tgctgcttct ggagttgttc cagtcatgaa attatttgaa gacagttttt cttactccaa
     1021 ccaacttgga caacgccaag gtgctggagt tgtttacctt gacgttttcc atccagatat
     1081 tatttcattc ttatcaacta aaaaagaaaa tgctgatgaa aaagtacgtg taaaaacatt
     1141 gtcattaggg attactgtac ccgataaatt ctatgaacta gcacgtaaca atcaagaaat
     1201 gtacttgttt agtccttata gtatcgaacg tgaatatggt gttcctttca gctatattga
     1261 tattaccgaa aaatacgatg aattagtcgc taaccctaat atcactaaaa caaaaatcaa
     1321 tgctcgtgat ttagaaacag agatttctaa acttcaacaa gaatcaggtt atccttacat
     1381 catcaatatt gacactgcta atcgtactaa tcctattgac ggaaaaatta tcatgtcaaa
     1441 tctttgctct gagattttgc aagtacaaaa accaagtctt atcaatgatg ctcaagaata
     1501 ccttgaaatg ggaactgata tttcttgtaa ccttggctca actaatgttt taaatatgat
     1561 gacatctcct gattttggaa aatcaattaa aacaatgact cgtgctctaa ctttcgttac
     1621 tgactcatca aatattgaag ctgttccaac tattaagaac ggaaatgctc aagcacatac
     1681 atttggactt ggagcaatgg gactccactc ctatttggca aaaaatcata tcgaatatgg
     1741 tagtccagaa tcaattgaat ttactgatat ttatttcatg ctaatgaact actggacctt
     1801 agtagaatca aacaatattg cccgcgaacg tcaaacaaca tttgtaggtt ttgaaaaatc
     1861 aaaatacgct gacggtacat actttgataa atatgtatct ggaaaatttg ttccacaatc
     1921 tgacaaagta aaatccctat ttgctaatca ttttatcccc gaagctaaag attgggaaaa
     1981 ccttagatac gcagttatga aagacggatt atatcatcaa aatcggcttg cagttgctcc
     2041 gaatggttct atttcgtata tcaacgattg ttcagcatct atccatccta ttacacaacg
     2101 tattgaagag cgtcaagaga aaaaaatcgg gaaaatttat tatccagcta atggcttagc
     2161 tacagatact atcccttatt acacttctgc ctatgatatg gatatgcgta aagtaataga
     2221 cgtctatgcc gcagcaactg agcacgtaga ccaaggtctc tcaatgacac tctttttacg
     2281 aagcgaatta ccaaaggagt tgtacgagtg gaaaacagaa agtaagcaga ctactcgcga
     2341 cctttcaatc ctacgtaact atgctttcaa caaaggtgtg aaatcaatct actatatccg
     2401 tacttttaca gatgatggta gcgaagtcgg agctaatcaa tgcgaaagtt gtgtcatcta
     2461 acgaagaaac tctacttaaa acatcagaaa ctagactgag ataaactatc tcagtttcat
     2521 gatgttatct tattttctaa ggtattttca tctttatttt tctaaaaatc gataatactt
     2581 gaaatattgc tagatcagaa ttctatattc tactcttctt ccatcatcat tatataaaaa
     2641 cctatttacc attggagaaa accatgacaa cttactacga agctataaac tggaacgaaa
     2701 ttgaagatgt tattgataaa tcaacttggg aaaaactaac cgaacaattt tggctcgata
     2761 cacgtatccc tttatcaaat gacttagacg attggcgcaa actttctgct caagaaaaag
     2821 atcttgttgg caaggttttt ggaggcttaa ccctacttga taccatgcaa tcagaaactg
     2881 gtgttgaagc tattcgtgcc gatgttcgca cgcctcacga agaagctgtc ttaaacaata
     2941 ttcaattcat ggaatctgtt cacgctaaat cttattcttc aattttctca actttaaata
     3001 ctaaatcaga aattgaagaa attttcgagt ggactaataa taatgagttc cttcaagaaa
     3061 aagcacgtat tatcaatgac atttatgcta atggaaatgc ccttcaaaaa aaggtggctt
     3121 ccacctacct cgaaactttc cttttttatt ctggcttttt cacacctctt tactatttgg
     3181 gaaataataa gttagcaaat gttgctgaaa tcattaaatt aattattcgt gatgaatctg
     3241 tacatggtac ttatatcggt tacaaattcc agcttggttt taacgaatta ccagaagatg
     3301 agcaagagaa ttttcgtgat tggatgtatg acctccttta tcagctgtat gaaaacgaag
     3361 aaaaatacac caagacactt tatgatggcg taggatggac tgaagaagtt atgacctttt
     3421 tacgctacaa tgctaataaa gctcttatga atttaggaca agatccttta ttcccagata
     3481 cagcaaatga tgtcaaccca attgttatga atggtatttc aacaggaaca tcaaaccatg
     3541 acttcttctc tcaagtaggt aatggttacc tacttggtag cgttgaagct atgcatgatg
     3601 atgactataa ctatggatta taagaaaaag acactgattt agtgtctttt tctagttaaa
     3661 tacaaatttc ttgacaagca aaccctttca agatagaatg ttagagtaaa tacacaaggg
     3721 agtgccttga gctgagattg cagatatgca aaatcctcta acctgatctc gttaggacga
     3781 gcgtaggaat tgtggtttga attggttaaa catgacaaat gacaaaagaa tctcctttgc
     3841 tgtataaaaa aaggagattt tttatgtcaa aaaataacaa cactacctgt cttattgaaa
     3901 ctgctatttt tgcagcgctt gctatggctt tatcaatgat tcctgatttt gcgtcatggt
     3961 tcactccctc ttttggagca attcctctga tactttttgc attacgtcgt ggtacaaaat
     4021 acggtctatt tgctggctta atttggggct tattgcactt tgttctttcc aaagtatatt
     4081 accttagttt atcccaagtt tttatagaat acatcttagc atttataagt atgggacttg
     4141 caggcgtatt ttctgctaaa tttaaagatg cacttagcag cagctctaaa actaaagctt
     4201 tatcattagc attatctgga gcaatattag caacatttgt tcgttatgtt tggcattata
     4261 tcgctggtgt tattttttgg gctagttatg caccaaaagg tatgtctgct acattatact
     4321 cattaagcgt caatggtact gctggtttgt taact
//
LOCUS       DATRTN010000116         4337 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.116, whole genome shotgun sequence.
ACCESSION   DATRTN010000116 DATRTN010000000
VERSION     DATRTN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.116"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            193..1221
                     /locus_tag="VBE22_001842"
     CDS             193..1221
                     /locus_tag="VBE22_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO7203272.1"
                     /translation="MVTIKDVAAKAGVNPSTVSRVLKDNASISSKTKERVKKAMEELG
                     YVPNVAAQMLASGLTQNIGVIFPPLVLPDRLSEPFFMEILSTLTNEAKEHGFTVSIAT
                     SMSSEELLEQVKLMHLQKRVDGFIVLYSDQKDPIREYLLKYKLPFVVVGAANGYENEI
                     TYIDNDNQLMGKSAIDYLYKKGHKSIQFVTDDLNSEVSEERYLGYFKGARKLGLNQKP
                     ALLFDRGNPQVLEEFINRVKEEETTALIVIGDTVSVRVMQFLSFYKLKVPDDISIMTF
                     NNSLFSHLIHPYLSTFDINVNNLGRTSVRRLIDIIKSPDKVFSETIIVPFTLEERESV
                     RDISKNNN"
     gene            1342..2838
                     /gene="malQ"
                     /locus_tag="VBE22_001843"
     CDS             1342..2838
                     /gene="malQ"
                     /locus_tag="VBE22_001843"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEO7203273.1"
                     /translation="MKKRASGVLMHITSLPGDLGIGTFGREAYAFVDFLVETDQKFWQ
                     ILPLTTTSFGDSPYQSFSAVAGNTHLIDFDLLTLEGFISKDDYQNISFGQDPEVVDYA
                     GLFEKRRPVLEKAVKNFLQEERATRMLSDFLQEEKWVTDFAEFMAIKEHFGNKALQEW
                     DDKAIIRREEEALAGYRQKLSEVIKYHEVTQYFFYKQWFELKEYANDKGIQIIGDMPI
                     YVSADSVEVWTMPELFKLDRDKQPLAIAGVPADDFSDDGQLWGNPIYNWDYHKESDFD
                     WWIYRIQSGVKMYDYLRIDHFKGFSDYWEIRGDYQTANDGSWQPAPGPELFATIKEKL
                     GDLPIIAENLGYIDERAERLLAGTGFPGMKIMEFGFYDTTGNSIDIPHNYTENTIAYA
                     GTHDNEVINGWFENLTVEQKAYAENYMRRLPNEPITETVLRTLYATVSQTTITCMQDL
                     LDKPADSRMNMPNTVGGNWQWRMRKEDLTENRKAFLKEITTIYNRGNK"
     gene            2850..>4337
                     /locus_tag="VBE22_001844"
     CDS             2850..>4337
                     /locus_tag="VBE22_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEO7203274.1"
                     /translation="MTRNFTTYVGQQGKVLSELTNEEIYVELLNFVKEEAAAKSKNSS
                     QRKVYYISAEFLIGKLLSNNLINLGIYKDVKKELELVGKSIAEIEDVEPEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFRNNQQEAEANYWIENNSWLVPTDI
                     SYDVPFRDFTLKSRLDRIDVLGYKKDTKNYLNLFDIDGLDYNLIEKGITFDKTEIKKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAIERGSNLHDLAEYAYVQINDT
                     HPSMVIPELIRLLTEKHGFEFDEAVSVVRNMVGYTNHTILAEALEKWPLEYLNEVVPH
                     LVTIIKKLDQMIREEQTNPEVQIIDEAGRVHMAHMDIHFSTSVNGVAALHTEILKNSE
                     LKVFYDIYPDKFNNKTNGITFRRWLEFANQDLADYLKELIGDSYLTDATQLEKLLTYA
                     DSNEVHDKLAAIKFKNKLALKRYLKENKGIELDEYSIIDTQIKRFH"
BASE COUNT         1494 a          643 c          836 g         1364 t
ORIGIN      
        1 gtcaagtctt tttttaaaaa aaataagaaa aataaaaaaa taatatcaat taatactaaa
       61 tgagaagatt attttagtga gagtattaca tttgcagaga aggcaaacgt ttgttttttt
      121 cttagattag agtataatag acaaaggact ataccaaaag gtataaaatg attagaatga
      181 agaggtcgta ggatggtaac tataaaagat gttgctgcca aagctggtgt taatccatcg
      241 acagtaagtc gtgtactaaa agataatgca tctatttcaa gtaagacgaa agagcgcgtg
      301 aaaaaagcta tggaagagct gggctatgtt cctaatgttg cagcacaaat gttggctagt
      361 ggtttaacgc aaaatattgg agttattttt cctccactgg ttttaccaga ccgcttaagt
      421 gaaccctttt tcatggagat tctttctaca ttaactaatg aagcaaaaga gcatgggttt
      481 acagtttcta ttgcaacaag tatgtcttct gaggagttat tagaacaagt aaaattaatg
      541 catctacaaa aacgagtgga tggttttatt gttctctatt cggatcaaaa agatcctatc
      601 agagagtatt tattaaaata taaactgccc tttgttgttg ttggtgctgc taatggctat
      661 gaaaatgaga ttacttatat tgataatgat aatcagctga tggggaaatc tgccattgat
      721 tatctctata aaaaaggaca taaaagcatc cagtttgtta cagatgattt aaattcagaa
      781 gtatcagaag agcgctattt agggtatttt aaaggtgcta gaaagttagg tttaaaccaa
      841 aagccagctc ttttatttga cagaggcaat ccgcaagttt tagaggaatt tataaataga
      901 gtcaaagaag aagaaacaac tgctttaatt gttattggtg atactgtctc tgttagagta
      961 atgcaatttc tttctttcta taaattaaaa gttccagatg acatttctat catgaccttt
     1021 aataattctc tcttctcaca tctgattcat ccttacctaa gcacatttga tattaatgtt
     1081 aataatctag gaaggacaag cgttcgtcgt ctgattgaca tcattaaatc tccagacaaa
     1141 gtctttagtg agactattat tgtgcctttt actttagaag agcgtgaaag tgtccgagat
     1201 atttctaaaa ataataatta agctatgata taaagacttc tatcaatttt cgatagaagt
     1261 ttttttaaat tatgcttgac ttatgcaatc gcttgcgtta agatggagga gtaaagtaag
     1321 taatcgaaaa ggagaatcaa tatgaaaaaa cgtgcaagtg gtgtcttaat gcacatcact
     1381 tctctaccag gtgatttagg tattggaaca tttggacgag aagcctacgc ttttgttgat
     1441 tttttggtcg aaactgatca aaaattttgg caaattcttc ccttaacaac aacaagcttc
     1501 ggggactctc cttaccagtc tttttctgca gtagctggta atacacattt aattgatttt
     1561 gatttgctaa cgcttgaagg tttcatttcg aaagatgact accaaaatat tagttttgga
     1621 caggatccag aagttgttga ttatgctggt ctgtttgaaa aacgccgtcc agttttagaa
     1681 aaagcagtta aaaatttctt gcaagaagag agagctacga gaatgctatc tgatttcttg
     1741 caagaagaaa aatgggtaac tgattttgct gaatttatgg cgatcaaaga acattttggt
     1801 aataaggcgc ttcaagaatg ggatgacaag gctattatac gccgcgaaga agaagcctta
     1861 gcaggatatc gtcaaaagct tagtgaagtg ataaaatatc atgaagtaac gcaatatttc
     1921 ttttacaaac aatggtttga gttaaaagaa tatgctaatg ataaagggat tcaaattatc
     1981 ggtgatatgc caatctacgt ttctgccgat agtgtagaag tttggacaat gcctgaactg
     2041 tttaaattag atcgcgacaa acaaccactt gctattgctg gtgtaccagc ggatgatttt
     2101 tctgacgatg gtcagctttg gggcaatcct atttacaatt gggattacca caaagagtca
     2161 gattttgact ggtggattta tcgtattcaa tctggtgtta agatgtatga ctatttgcgt
     2221 attgatcatt ttaaaggttt ttcagattat tgggaaattc gaggagatta tcaaacagct
     2281 aatgatggct catggcaacc agctcccggt ccagaattat ttgcaactat caaagaaaaa
     2341 ttaggtgatt tacctattat tgctgagaat ttaggctata ttgatgagag agcagagaga
     2401 ttactggctg gaacagggtt cccaggtatg aaaatcatgg aattcggttt ttatgataca
     2461 acaggaaata gtattgatat tcctcataac tacacagaaa atacaattgc atatgctgga
     2521 actcatgata acgaagttat taatggctgg tttgaaaatc ttacagtcga acagaaagct
     2581 tatgcagaaa attacatgcg tcgtttgcca aatgagccca ttactgagac tgtactgcgt
     2641 accttgtatg caaccgttag tcaaacaacg attacatgta tgcaagatct actagataaa
     2701 cctgcagata gccgtatgaa catgccaaat accgttggag gtaactggca atggcgtatg
     2761 agaaaagaag acttaacaga aaatcgcaaa gcattcttaa aagagattac aactatttat
     2821 aatagaggga ataaataagg aatattatca tgacaagaaa ttttacaact tacgttggac
     2881 aacaaggaaa agtattatca gaattaacaa atgaagagat ttacgttgaa ttgttaaatt
     2941 ttgtaaaaga agaagctgcc gctaaatcaa aaaatagctc tcaacgtaag gtatactata
     3001 tttcagctga gtttctaatt ggtaaattac tttcaaataa tttgattaac ttagggattt
     3061 ataaagatgt caaaaaagag ttagaacttg taggtaaatc aattgctgaa atagaagatg
     3121 tagagccaga accatcactt ggtaatggtg gtttagggcg tttagcatca tgttttattg
     3181 actcgatttc ttctttaggt attaatggtg aaggtgttgg tttaaactat cactgtgggc
     3241 ttttcaaaca agtatttcgc aacaatcaac aagaagcaga agctaactac tggattgaga
     3301 ataattcttg gttagtacca acagatattt cttatgatgt tcctttccgt gactttactt
     3361 taaaatcacg cctagataga attgatgtat taggttataa gaaagatact aaaaattatt
     3421 tgaacctttt tgatattgat ggacttgatt ataaccttat tgaaaaaggt atcacttttg
     3481 ataagacaga aatcaagaag aatttaacct tgttcttgta tccagatgat tcagataaaa
     3541 atggagaatt attacgtatc tatcagcaat atttcatggt ttcaaatgct gctcaattat
     3601 taattgatga agcaatcgag cgtggttcaa accttcatga tttagctgaa tatgcttatg
     3661 ttcaaattaa tgacactcac ccatctatgg tcatccctga gttgattcgt ttattgacag
     3721 aaaaacatgg ttttgaattt gacgaagctg tgtcagttgt tcgcaatatg gttggctata
     3781 caaaccatac catccttgca gaagctcttg aaaaatggcc tttagagtat cttaatgagg
     3841 tagtacccca cctagtaaca atcattaaaa aattagatca aatgattcgt gaagagcaaa
     3901 caaatccaga ggttcaaatt attgatgaag ctggacgtgt tcacatggcg catatggata
     3961 tccacttctc aacaagcgtt aatggtgtag cagcattaca tacagaaatc cttaaaaata
     4021 gtgaattaaa ggttttctat gatatttacc cagataagtt taacaataag acaaacggta
     4081 ttacattccg tcgctggttg gagtttgcta accaagattt agcagattac ttgaaagaac
     4141 taattgggga tagctatctt actgatgcga ctcagctcga aaaattgtta acttatgctg
     4201 atagcaatga agttcatgat aaattagctg ctattaaatt taaaaacaaa ctggctctta
     4261 aacgttacct taaggaaaat aaaggtattg aattggatga atactcaatt attgatacac
     4321 aaatcaaacg attccac
//
LOCUS       DATRTN010000117         4287 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.117, whole genome shotgun sequence.
ACCESSION   DATRTN010000117 DATRTN010000000
VERSION     DATRTN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.117"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            21..1052
                     /locus_tag="VBE22_001845"
     CDS             21..1052
                     /locus_tag="VBE22_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO7203275.1"
                     /translation="MKLSKQLYKSLPLLTVVLCVGALQQNVEAKAKHYKTTSHVETQY
                     VSTSSKKILPFTHNKQIKVGPLDNLGRATYSHIQLRDADEPKIKRERLTYNPTGWHNY
                     KFTTEKGKTTWLMDRGHLVGYQFSGMNNVPENLVTMTKYLNTGFSENNPDGMLYYENR
                     LDSWLANHKNFWLDYKVTPIYEGNNLVPSRVELQYVGIDKQGKLLEIKLGGGKEQTDE
                     YGVTTVTLENTSPLAKIDYKTGMLIKEDGKQAEEGEDPNSDADENEAAIESASDIEEN
                     TNTNTSESDTNNVAPKNRIVYVANKGRSNTYWYSLENIKNANTANIVQMTEQEALNQH
                     KHHSTTEAQ"
     gene            complement(1264..2076)
                     /locus_tag="VBE22_001846"
     CDS             complement(1264..2076)
                     /locus_tag="VBE22_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO7203276.1"
                     /translation="MLLDMLSYDFMQRALLAVVAISIFAPILGIFLILRRQSLMSDTL
                     SHVSLAGVALGVVLGISPTWSTIFVVTLAAVVLEYLRTVYKHYMEISTAILMSMGLAI
                     SLIVMSKAHNVGNVSLEQYLFGSIITIGKEQVIALFVIALITFILTILFIRPMYILTF
                     DEDTAFVDGLPVRTMSILFNVVTGIAIALTIPAAGALLVSTIMVLPASIAMRLGRNFK
                     TVIFLGMLIGFVGMVAGIFLSYYWETPASATITMIFIGIFLLVSLVGLLRKR"
     gene            complement(2066..2776)
                     /locus_tag="VBE22_001847"
     CDS             complement(2066..2776)
                     /locus_tag="VBE22_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO7203277.1"
                     /translation="MRYITVSGLTFQYDSDPVLEGVNYHLDSGEFVTLTGENGAAKST
                     LIKATLGILTPKVGTVNISKENKEGKKLRIAYLPQQIASFNAGFPSSVYEFVKSGRYP
                     RNGWFRRLTKHDEEHIRVSLEAVGMWDNRHKKIGSLSGGQKQRAVIARMFASDPDIFV
                     LDEPTTGMDAGTTEKFYELMHHNAHKHGKSVLMITHDPDEVKGYADRNIHLVRNQSLP
                     WRCFNVHTNEMEVESDAT"
     gene            complement(2779..3222)
                     /locus_tag="VBE22_001848"
     CDS             complement(2779..3222)
                     /locus_tag="VBE22_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEO7203278.1"
                     /translation="MTVLEQKLDHLVSQILLKAENQHELLFGTCQSDVKLTNTQEHIL
                     MLLSQEQLTNSDLAKKLNISQAAVTKAVKSLISQDMLKANKDSKDARITYFELSELAK
                     PIADEHTHHHDNTLGVYGRLVNHFSKDEKVVLERFLDLFSRELEG"
     gene            complement(3308..4159)
                     /locus_tag="VBE22_001849"
     CDS             complement(3308..4159)
                     /locus_tag="VBE22_001849"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEO7203279.1"
                     /translation="MRIFEKAPAKLNLGLDIKGRCDDGYHELAMIMVSIDLNDYVTIS
                     ELKEDCIVIDSDSSKMPLNNDNDVFKAADIIKNQYGINKGVHIRLEKSIPVCAGLGGG
                     STDAAATIRALNRLWNLQMDYDEMVAIGFKIGSDVPYCLGGGCSLVLGKGEIVKPLPT
                     LRPCWIVLVKPDFGISTKSIFRDIDCKSISRVDIDLLKSAILSSDYQLMVKSMGNSLE
                     DITITKNPVISTIKERMLNSGADVALMTGSGPTVFSMCSTEKKADRVFNSMKGFCKEV
                     YKVRLLR"
BASE COUNT         1577 a          845 c          633 g         1232 t
ORIGIN      
        1 atttagaagg gtttaggttt atgaaattat ctaaacaatt atacaaatca ctcccactat
       61 taactgtcgt gctatgcgtt ggggcattac aacagaatgt tgaagctaag gctaaacatt
      121 ataagacgac atcccatgtt gagacacagt atgtaagtac ttcttcaaaa aaaatcttac
      181 catttactca taataaacaa atcaaggtag gtccattaga taacttaggc agagcaacat
      241 attctcacat acaattgagg gatgcagatg agcctaagat aaaaagagaa agactgacgt
      301 ataatccaac cggttggcac aactacaaat ttactactga aaagggtaaa acaacttggc
      361 ttatggaccg aggtcacctc gttggttatc aattttcagg aatgaacaat gttcctgaga
      421 acctagttac tatgaccaaa tatctaaata caggatttag cgaaaataat cctgatggta
      481 tgttatacta cgaaaataga ttagatagtt ggttggcaaa tcataaaaat ttctggcttg
      541 attacaaagt gactccaata tacgaaggaa acaatttagt acctagccga gttgaacttc
      601 agtatgttgg aattgataaa caaggaaaac ttctagaaat caaattaggc ggcggcaaag
      661 aacagactga tgaatacggc gtcacaactg ttacattaga aaatacgtct ccactcgcaa
      721 aaattgatta caaaactggt atgctaatca aagaagatgg gaaacaggca gaagaaggtg
      781 aagatccaaa tagcgatgct gatgaaaacg aagcagctat tgaaagtgca tctgatatag
      841 aagaaaatac taacactaat acttctgaat cagatacaaa taatgtagct cccaaaaata
      901 gaatcgtcta cgttgcaaat aagggtcgtt caaatactta ttggtatagt ttagaaaaca
      961 taaaaaatgc aaataccgct aatatcgttc aaatgactga acaagaagcg ttaaatcaac
     1021 acaaacatca cagcactact gaagcacaat aattacaatt tactgccggc aaaattgccg
     1081 gtagtatttt ttatcctaaa acgcaaaaat agattcacat tgccccttct atcaacttaa
     1141 gccagaaaac ttagtagata cctcatcgag ataatttctc aactgatccc tcataaaata
     1201 tattcgaaaa aaaacaggga gataaataaa aaatctatcc tagaataaga tagatttttt
     1261 tatttaacgt ttgcgaagca agcctactaa gctaactaat aggaaaatgc caataaaaat
     1321 catcgttatt gttgcactag caggtgtctc ccaataatac gaaaggaaaa taccagctac
     1381 cattcccaca aatccaatta acatacccag aaaaataaca gttttaaaat ttcgacctaa
     1441 ccgcatagca atactagctg gtaaaaccat aatagttgac accaatagag cacctgcggc
     1501 aggaattgtt aatgcaatag caatacctgt aacaacattg aacaaaatag acattgtacg
     1561 cacaggcaat ccatctacaa aagctgtatc ctcatcaaac gttaatatat acattggtct
     1621 aataaacagg atggttaaaa taaaagtgat taatgcaata acaaacaaag caatcacttg
     1681 ttccttccca atagtgataa tagaaccaaa taaatactgt tcaagactga cattaccaac
     1741 attatgagct ttactcataa cgattaaaga tatagccaac cccatcgaca tcaggatagc
     1801 agttgatatt tccatataat gtttgtatac agtacgcaaa tactctaata caactgccgc
     1861 caaagtaaca acgaaaatag ttgaccatgt aggcgagata cctaaaacaa cgcctaatgc
     1921 aacaccggct aaggaaacgt ggctcaaagt atcactcatc aaactctgac gacgtaaaat
     1981 taggaagatg cctaaaattg gagcaaaaat actaatagcc acaactgcta agagcgctcg
     2041 ttgcataaaa tcataggaaa gcatatcaag tagcatcaga ttcaacctcc atttcgtttg
     2101 tatggacatt gaaacaacgc catggtaaag attggttcct aacaagatga atatttcggt
     2161 cagcataccc ctttacttca tcaggatcgt gagtaatcat caaaacagac ttaccatgct
     2221 tatgagcatt atgatgcatt aactcataaa atttctcagt agtccctgcg tccataccag
     2281 ttgttggctc atccaataca aaaatatctg gatcagaagc aaacatccta gcaatgacag
     2341 ccctctgctt ttgaccgcca gataaacttc ctatcttttt atggcgatta tcccacatac
     2401 caacagcttc taaactaacc ctaatatgtt cttcatcatg tttagtcagt cttctaaacc
     2461 aaccatttct agggtatcgt ccagatttca caaattcata aactgacgat ggaaagccag
     2521 cgttaaaact tgcaatctgt tgaggtaaat aggcaatacg taattttttt ccttctttgt
     2581 tttctttaga gatattaact gtacccactt tgggcgttaa aatacctaaa gtagccttta
     2641 ttaaagttga tttagcagcc ccattttcac ctgttaaggt aacaaactcc ccactgtcta
     2701 aatggtaatt aaccccttcc agcacaggat cgctatcata ctgaaaagta agaccactaa
     2761 ctgtaatata tcgcatgatt acccttctaa ttctctagag aaaagatcaa gaaaacgttc
     2821 taaaacgacc ttttcgtcct ttgaaaaatg atttactaat cttccgtaaa cccctaacgt
     2881 attgtcatga tgatgtgtgt gttcatctgc aatgggttta gcaagttcag ataactcaaa
     2941 ataagtaata cgagcatctt tagaatcttt attcgctttc aacatatcct gagaaattaa
     3001 actttttact gctttagtta cagctgcctg actaatattt aacttcttag ctaaatcaga
     3061 atttgtcaac tgctcttgtg ataaaagcat cagaatgtgt tcttgcgtat tagtcaattt
     3121 tacatcactt tgacaagtac caaacaataa ttcatgttga ttttctgctt taagcaagat
     3181 ttgactcact aaatggtcta atttttgttc taaaactgtc atatatacct cttttttgtt
     3241 aaccagtaaa ttatatcacg aagatataga agaatcaatc atagataggt gaagaaggta
     3301 aaacctttta tctcaacaac ctaactttat aaacttcttt gcaaaaacct ttcatactat
     3361 taaaaacacg atcagctttt ttctctgtag aacacattga aaaaacagtt ggtccacttc
     3421 ctgtcattaa tgcaacatcg gctccagaat ttaacatacg ttcttttatt gtacttataa
     3481 ctggattttt agtaattgta atatcctcga gtgaatttcc catagatttg accattaact
     3541 gataatctga tgacaaaata gcagacttta ataaatcaat atcaactctg cttatagact
     3601 tacaatcaat atctctaaaa atggatttag ttgaaatacc aaaatccggc ttaaccagaa
     3661 ctatccaaca tggtctcaat gtcggtaagg gtttaacaat ttcaccttta cctaatacta
     3721 acgaacatcc cccaccaaga caataaggaa catcactacc aattttaaaa ccaatagcaa
     3781 ccatttcgtc atagtccatt tgaagattcc ataatcgatt aagagctctt attgtagcag
     3841 cagcatcagt agaaccaccc cccagtcctg cacagacagg aatggatttt tctaatctaa
     3901 tatgaacacc tttattaata ccatattgat ttttgattat atctgcagct ttaaacacat
     3961 cattatcatt atttaaaggc attttgctac tatcagaatc gataacaata caatcttcct
     4021 ttagctcaga aatggtaacg tagtcattaa gatcaatact aaccataatc atagctaatt
     4081 catgataacc atcgtcacat cgtcctttaa tatctaatcc taaattaagt ttggcaggag
     4141 ctttctcaaa aattctcata aaacctccct aataaaatat agaatatcca tattataaca
     4201 taacaaatga caaattcgga caattaagat gttagccaat cttaaggata ataattccaa
     4261 taaaaaaagg ctaaccaaag ttagtct
//
LOCUS       DATRTN010000118         4208 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.118, whole genome shotgun sequence.
ACCESSION   DATRTN010000118 DATRTN010000000
VERSION     DATRTN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.118"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            131..1870
                     /locus_tag="VBE22_001850"
     CDS             131..1870
                     /locus_tag="VBE22_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7203280.1"
                     /translation="MTLFLIMMERAGLIILLAYAFVHIPFIKQTLKQPELKKHQYILL
                     ILFSLFAIISNFTGVEIQSDLSIIPQTLNHIADQSSVANTRVLTIGVSGLIGGPIVGI
                     IVGLLSVFVRYLQGGLAPHIYVISSLLIGLCSGLSGNYLRKNYNKIRVLDAMVVGFGM
                     EILQMICILIFSVDFNQAFRLVSFISMPMILSNTLGLGIFISIISSTQKLEEHAKAFQ
                     THQVLELANLTLPYLRKGLTTESCQPVAEIIHKHMDVSAVSLTSQSAILAYVGDGADH
                     HLPNTQILTKLAKRAIDTGKVSVATDKSEIECDHNNCPLSSAIVIPLHIHDVIVGTLK
                     LYFSDAQHMTYVDRQLAEGLGNIFSTQLALGQAEEATRLLQDAEMKSLQAQVNPHFLF
                     NALNTIYGLIRMDSEKARKLVQDFSKVIRANLQRAKQNLIPLHDELEQVNAYLALEEA
                     RFPNMVAFNLDNQTNSDDNLMIPPFTLQVLIENSYKHAFKHINKNNQLKVTITRNNDR
                     LHIIVQDNGIGIPKEKLITLGKKTQISKQGSGTAIENLVRRLNIIYDGQASLKFESND
                     SGTCAIVNIPIKN"
     gene            1882..2616
                     /gene="ltdR"
                     /locus_tag="VBE22_001851"
     CDS             1882..2616
                     /gene="ltdR"
                     /locus_tag="VBE22_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LtdR"
                     /protein_id="HEO7203281.1"
                     /translation="MKVLVVDDEPVARNELIYLLNKYDSNLVIAEAHDMATALAILLR
                     ETFDVALLDIHLRDDSGLQLAEYINKMPKPPLLIFATAYDQYAIQAFEHDARDYLLKP
                     YDFDRLKQAMDRVKGALSTSTIIESVTSGPLFKQQYPLTVEDRIYLVSADDILLIEAM
                     QGKLIIQTPDKNYEIDGSLQQWQDKLPSSQFVRVHRSYIVNINAIKTIEPWFNQTLQL
                     HLCNKITVPVSRANVKPLKQMLGIST"
     gene            2772..>4208
                     /locus_tag="VBE22_001852"
     CDS             2772..>4208
                     /locus_tag="VBE22_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEO7203282.1"
                     /translation="MVTFLGGVALLIVGYFTYGRYIEKNFQIDENRQTPAEALRDGYD
                     FVPMPKWKNGMIELLNIAGTGPIFGPILGALYGPVAYIWIVLGCIFAGAVHDYMIGMI
                     SLRNNGAYLPELASRYLGKSMKHVINIFSMLLLILVATVFVVTPANLILSILPAGTLS
                     LPWIIGLIFVYYLISTVLPIDKALGKVYPVFCVILMVSTAAVGFRLLTGGFDMPNLTF
                     ETFKNMHPAGLGIFPALFFTISCGAISGFHATQAPMVSRTTVNEREGRFTFYGMMIAE
                     GVIAMIWAGASMSLFKGQNLYEMIAAGTPSAVVNQVMLMLLGSVIGTIAIIGVIVLPV
                     SSGLSAFRSLRTIVADYIHVKQDTLPKIFAVTIPLYVISFVLTHVDFNLLWRYFNWAN
                     QVTAVIGLLVATRYLILKRRNYWVTFVPAMFMLYAVVVYILSQPIGFNMGLGILTYSL
                     ALVLTGILVGLFWKSGQKQLKTVHPEAFL"
BASE COUNT         1310 a          730 c          746 g         1422 t
ORIGIN      
        1 aatagttgtt gatggatatc cccttaattg tcttgagaat acttgataga gagacatttt
       61 aaatgagtaa tgaaatgaat cattttatac ctatgttata acggactaat taatactttg
      121 gagagattta atgacactct ttttaattat gatggagcga gcggggttaa tcatattatt
      181 agcctatgcc tttgtgcata tcccctttat aaaacaaact ttgaagcaac ccgaattaaa
      241 aaaacaccag tatattctgc ttatcttatt tagcttattt gctattattt ctaattttac
      301 aggggtagaa attcagtcag atttatctat cattccacaa actcttaatc atatagcaga
      361 tcaatcatcg gttgccaaca cacgtgtttt aaccattggt gtatcaggtc taatcggtgg
      421 ccccatagtc ggtattatag ttggtctctt atctgtattc gttaggtact tgcaaggagg
      481 attagcccct catatttatg tcatttcttc cttactaatt ggtttatgct ctgggttatc
      541 aggcaattat cttcgtaaaa attataataa aattcgtgtt ttagatgcaa tggttgttgg
      601 tttcggtatg gaaattctac aaatgatctg tatcctcatt tttagtgttg actttaatca
      661 ggcgtttcga ttagtatctt ttatcagcat gccaatgatt ttaagtaata ctttaggttt
      721 aggtatattt atatccatta tttcatctac tcaaaaatta gaagaacatg ctaaagcatt
      781 tcaaacccat caagtacttg aattagcaaa tttaaccctt ccttacctta gaaagggact
      841 gacaacagaa tcttgtcaac cagtagctga aattattcat aaacatatgg atgtgagtgc
      901 tgttagcctg actagtcaat cagccatttt agcctatgta ggagatggag ctgaccatca
      961 tcttcctaat actcaaatat taacaaagct tgctaaaaga gcaatagata ctggtaaagt
     1021 tagtgtcgca actgataaaa gtgaaatcga atgcgaccat aacaattgcc cacttagttc
     1081 agcaattgtt attccattac acatccacga tgttatagtg ggtactttga aattatattt
     1141 ttcagacgca caacatatga cttacgttga ccgtcaatta gctgaaggat taggcaatat
     1201 cttttccact cagttagctt tgggacaagc tgaggaagca acaaggctac tacaggatgc
     1261 ggaaatgaag tctctccaag cgcaggttaa tccgcatttt ctttttaatg cactcaatac
     1321 tatttatggt ttaattcgca tggatagcga aaaagcaaga aagctcgtcc aagattttag
     1381 caaggttatt cgtgctaatc ttcaacgggc aaagcaaaat ttaattcctc tccatgatga
     1441 attggaacag gttaacgcct atttagccct tgaagaagca agatttccta acatggtggc
     1501 ctttaattta gacaaccaaa caaattcaga tgacaactta atgattcctc cctttacatt
     1561 gcaggtcttg attgaaaact cttataaaca cgcttttaag catattaata aaaataatca
     1621 gctaaaggtt actattacaa gaaataatga taggcttcat atcattgtcc aagacaatgg
     1681 tattgggatt ccaaaagaaa aattaataac gcttggaaaa aaaactcaaa tttcaaaaca
     1741 aggttctggt acagctattg agaatctcgt tcgtcgcttg aatattattt atgatggaca
     1801 agcaagttta aaatttgaat caaatgattc aggaacttgt gctattgtta atatccccat
     1861 taaaaattag aaaggtattg tatgaaagtt ttagtagttg atgatgaacc agttgcacgt
     1921 aacgaattaa tttaccttct taataagtat gattctaacc tcgttatagc agaggcgcat
     1981 gatatggcta ctgcattagc tattttactt agagaaactt ttgatgtagc actgttagat
     2041 atccatctca gagatgattc tgggttgcaa ttagcagagt atatcaataa aatgcccaaa
     2101 ccaccattat tgatatttgc gactgcttat gatcaatatg ctattcaggc ttttgagcat
     2161 gatgcgcgtg attatttgtt aaaaccctat gattttgata ggctaaagca agctatggat
     2221 agagtaaaag gagcgctaag tacatctaca attatagaga gcgtaacttc cggtcctctc
     2281 ttcaagcaac agtatccatt gacagtagaa gatcgaatct atctggtgtc ggcggatgat
     2341 atccttttga ttgaagctat gcaaggaaaa ctgattatac aaacacctga taaaaattat
     2401 gaaattgatg gctctctaca acaatggcaa gataaactac catcatctca atttgtacgg
     2461 gtacatcgct cttacattgt gaacattaat gctattaaaa cgattgaacc ttggtttaac
     2521 caaacacttc agttacacct ttgtaataaa ataacagttc ctgttagcag agcaaatgta
     2581 aaacccctaa aacaaatgtt aggcatatct acctaactaa tgaaaagtta ttcatcataa
     2641 aacttagact attcatttct aaaaaacaac cattcatctt gaaaatagga aatttcaatt
     2701 tttcaatgct atactaaagt catgaaaacg cttaataaaa ttaagcaaaa aaatttttag
     2761 gaggcagtga tatggtaaca tttttaggag gggtagccct tttaattgtt ggttatttca
     2821 cgtacggtag atacattgaa aagaattttc aaattgatga aaatcgccaa acacctgcag
     2881 aagcattaag ggatggatat gattttgttc ctatgccaaa atggaagaat gggatgattg
     2941 agttattgaa catcgcagga accggaccta tctttggacc tattttagga gcactctatg
     3001 gtccagttgc ttatatctgg atagtcttag gatgtatttt tgcaggggct gttcatgatt
     3061 atatgattgg aatgatttct cttagaaata atggtgcata tcttcctgaa ttagctagtc
     3121 gctatcttgg aaagtcaatg aagcatgtga tcaacatttt ttcaatgcta ttacttattt
     3181 tagttgcaac cgtatttgtg gtaactccag ctaatcttat cctaagtatt ttaccagctg
     3241 ggacactatc tttaccttgg ataatcggtc ttattttcgt ttattacctt atttctacag
     3301 tcttacctat tgataaagca cttggtaaag tttatcctgt attctgcgtc atcttaatgg
     3361 ttagtacggc agcagttggc ttccgtttat taactggtgg ctttgatatg ccaaacttaa
     3421 cttttgaaac ctttaaaaat atgcatccag ctgggcttgg gatattccct gctttattct
     3481 ttaccatttc atgtggagcc atctctggtt tccatgctac ccaagcacct atggtctcaa
     3541 gaacaactgt taatgagaga gaagggcgtt tcactttcta cggcatgatg attgcagaag
     3601 gagtaattgc gatgatttgg gcaggtgctt caatgagctt gttcaaaggt caaaatctct
     3661 atgaaatgat tgctgctggg acaccttctg cagttgtcaa tcaagtaatg ttaatgttac
     3721 tcggtagtgt aattggaact atcgctatca ttggtgttat tgttctccct gtttcatctg
     3781 gtttatcagc tttccgtagc ttacgtacta tcgttgctga ttatatccat gtcaaacaag
     3841 atactcttcc aaaaatcttt gctgtaacta tcccattata tgttatttca tttgtcctta
     3901 cacacgttga ttttaatctc ctatggcgtt actttaactg ggcaaatcaa gtaactgctg
     3961 ttattggttt attagttgct acaagatatc tcatcctaaa aagacgaaat tattgggtaa
     4021 catttgtacc agctatgttc atgttgtatg cagtagttgt ttatatttta tctcaaccaa
     4081 ttggatttaa tatgggctta ggcatcttaa cttattcact tgctttagtg ttaacaggta
     4141 ttcttgttgg acttttctgg aaatctggtc aaaaacaatt aaaaactgtt catcctgaag
     4201 cattcctg
//
LOCUS       DATRTN010000119         4145 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.119, whole genome shotgun sequence.
ACCESSION   DATRTN010000119 DATRTN010000000
VERSION     DATRTN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.119"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(1..802)
                     /locus_tag="VBE22_001853"
                     /pseudo
     CDS             complement(1..802)
                     /locus_tag="VBE22_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037479.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
     gene            complement(945..2321)
                     /locus_tag="VBE22_001854"
     CDS             complement(945..2321)
                     /locus_tag="VBE22_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEO7203283.1"
                     /translation="MAYKTIYPYTNEVLHEFDNISDSDLEQSLDIAHALYKTWRKEDN
                     VEERQNQLHKVADLLRKDRDKYAEVMTKDMGKLFTEAQGEVDLCADIADYYADNGQKF
                     LKPVPLESPNGEAYYLKQAVGVLLAVEPWNFPFYQIMRVFAPNFIVGNTMLLKHASIC
                     PASAQAFEDLVREAGAPEGAFKNIFASYDQVSNLISDPRVAGVCLTGSERGGASIAAE
                     AGKNLKKSSMELGGNDAFLILDDADFDLLSKTIFFARLYNAGQVCTSSKRFIVMADKY
                     DEFVNMVVETFKSAKWGDPMDSETTLAPLSSAGAKDDVLKQIKLAVDHGAEVVFGNDT
                     IDHPGNFVMPTVLTNITKANPIYNQEIFGPVASIYKVDTEEEAIALANDSSYGLGSTV
                     FSSDPEHAKKVAAQIETGMTFINSGWTSLPELPFGGIKNSGYGRELSQLGFDAFVNEH
                     LVFTPNSD"
     gene            complement(2395..3402)
                     /locus_tag="VBE22_001855"
     CDS             complement(2395..3402)
                     /locus_tag="VBE22_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEO7203284.1"
                     /translation="MLFKEKIPGLILCFIIAIPSWLLGLYLPLIGAPVFAILIGIIVG
                     SFYQNRQLFNKGIAFTSKYILQTAVVLLGFGLNLMQVMKVGISSLPIIIMTISISLII
                     AYVLQKLFKLDKTIATLIGVGSSICGGSAIAATAPVINAKDDEVAQAISVIFLFNILA
                     ALIFPTLGNFIGLSDHGFALFAGTAVNDTSSVTATATAWDAINHSNTLGGATIVKLTR
                     TLAIIPITIVLSIYHMKQTQKEQSVSVTKIFPKFVLYFILASLLTTIVASLGFSLRIF
                     EPLKVLSKFFIVMAMGAIGINTNVSKLIKTGGKSILLGAACWLGIIIVSLTMQAILGT
                     W"
     gene            complement(3510..>4145)
                     /locus_tag="VBE22_001856"
     CDS             complement(3510..>4145)
                     /locus_tag="VBE22_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203285.1"
                     /translation="VGAGGLYFWNNHQSLEGKWRTVSLEKQVEKEIEQQLGSQAADMG
                     ISAADLVKGANMHMNVKNDEAKITVTAQIDEVKFHQAIKTFIDKALEKQLKDQGLTYN
                     DLSEAGKKIFDETKITDKQIDQQIDRSFQSAAQAAGGKYNTNTGEMTLPVMDGKVHRL
                     TSVIKVSHINKKANAFYGNIVKNGEKTAYKKEGSKLILGNEKSYPFMKVTK"
BASE COUNT         1383 a          851 c          640 g         1271 t
ORIGIN      
        1 ctaaagctaa gagggctacc gctaaaatca aatttaagtt cgataaaatt ttaacacctt
       61 ttcctaaacc tgataaagct gacataacaa ataaagctgt tgtaataagg ataataacaa
      121 tttgaacaaa tgcattattg ggaacaccca ataaaaaact taaccctccg ttgatttgag
      181 cagctccaaa tccaagtgtc gtagcaactc caataactgt agcaacaact gtggtgatat
      241 caacaatttt tcctagtcaa ccatctgtct tatcaccgaa aagaggcttt aaagtgacag
      301 acaagaggta tttctcttgc tttcgaaatc caaaataagc tagagccaaa gcaaccaatg
      361 catatactgc ccaagcatgt atcccccagt gaaaaaatgt aaaacgaaat gcatcagcta
      421 atgctgtttg cgattcctta ggtgcgccag gtgtcgaaat agcaaaatgc gataagggtt
      481 cagctgctcc gtagaagacc aaaccaatcc ccatacctgc tgaaaacatc attgctatcc
      541 atgatacagt taaatattca ggacgtgaat gaggtcgccc taaacgtatt tgtccaagag
      601 gactcataat aaaaaagaca cacaaagcaa gaattgaagt aactaacaac aaatacaact
      661 aaccgaaatt ggtagatacc tgttcacgca agatttgtgt ccaagattga aaaccacgag
      721 gaacaataat tcctaataag actaaaataa gcgatacaat tagtgaacct gtaaacacag
      781 gcgtaatatg ttttttactc aaaatttcct cctatactaa aatagtatag catactttaa
      841 taaatactcc tctcatttta atcacctgta tcaagacctc tccttgtctc ctcaacaaaa
      901 aagaaccaga taacattatc tggttcttgt gtttgactta gtgattaatc actatttggt
      961 gtaaatacca aatgttcgtt gacaaaggca tcaaatccaa gttggctcaa ttcacgacca
     1021 tatcctgaat ttttaatacc tccaaacggt aattccggta atgatgtcca ccctgaatta
     1081 ataaatgtca tccctgtttc aatctgagcc gccacttttt tagcatgttc tggatcagaa
     1141 gagaaaacag tacttcctaa accataacta gaatcattag ctaaagcaat agcttcttct
     1201 tcagtatcca ctttatagat agaggctacg gggccgaaaa tttcttgatt atagattgga
     1261 tttgctttag tgatattcgt taaaacagtt ggcatgacga agtttcctgg atgatctata
     1321 gtgtcatttc caaaaactac ctcagcaccg tggtctaccg ccaatttaat ttgttttaaa
     1381 acatcatcct ttgcacctgc agatgacagt ggagccaagg ttgtttccga atccattggg
     1441 tcaccccatt tagcagactt aaaggtctca actaccatat ttacaaattc gtcatactta
     1501 tcagccataa caatgaaacg ttttgaagat gtacatactt gacctgcatt atataaacgc
     1561 gcaaagaata ttgttttact aagtaaatca aaatcagcat catctaaaat aagaaaagca
     1621 tcgttcccac ctaattccat agacgatttt ttcaaatttt taccagcctc agctgctatt
     1681 gatgccccac cacgctcaga accagttaaa cacacacctg caacacgagg atctgatata
     1741 aggtttgaaa cttgatcata cgaagcaaaa atatttttaa acgctccttc tggagctcct
     1801 gcttctcgga ctaaatcctc aaatgcttga gctgatgcag gacaaataga agcatgtttt
     1861 aaaagcatag tattacccac tataaagtta ggggcaaata cacgcataat ctgataaaat
     1921 gggaaattcc atggctcgac agcaagtaaa acaccgacag cttgtttcaa atagtaagcc
     1981 tctccatttg gactttctaa cggaacaggt tttaaaaact tttgcccatt atcagcataa
     2041 tagtcagcta tgtctgcaca caagtccact tctccttgtg cttcagtgaa aagttttccc
     2101 atatctttag tcataacttc agcgtactta tcacggtcct tacgtaataa atctgctact
     2161 ttatgcaatt gattttggcg ttcttcaaca ttatcttctt tgcgccatgt cttgtagagg
     2221 gcatgcgcta tgtctaatga ctgttcgaga tcactgtcac tgatattatc gaattcatgc
     2281 aatacttcgt tagtgtacgg ataaattgtt ttatatgcca taatgttccc tccttttgat
     2341 atcctcatta taacatttat gaaatcgatt acaaatattt agctttagac aagatcacca
     2401 agttccaagg atagcttgca ttgttaaact aactataata atgcctaacc aacacgctgc
     2461 acctaaaaga attgattttc ctccagtttt aattagtttg ctgacattag tatttatacc
     2521 aattgccccc attgccataa caataaaaaa tttagataaa accttaagtg gctcaaagat
     2581 acgtaacgaa aaacctaatg aagcaactat cgttgttagc aaagaagcta agataaaata
     2641 aagcacaaac ttcggaaata tcttagtaac acttacagat tgttctttct gcgtttgttt
     2701 catatgataa atagataata caatcgtaat tggaatgatg gctaatgttc gtgtcaattt
     2761 aacaatagta gctccgccca aagtattgct gtgattgata gcatcccatg cagtagcagt
     2821 tgctgtaact gacgaggtat cattgacagc agtccctgca aataatgcaa aaccgtgatc
     2881 agagagacca ataaaattcc ccagcgttgg aaaaattaaa gccgctaaga tattgaataa
     2941 aaaaataacc gaaatagctt gtgctacttc atcatcttta gcattaataa ctggtgcagt
     3001 cgctgcaatt gcggatccac cacaaataga tgaaccaaca cctattaaag tagcaatcgt
     3061 tttatctaac ttaaataact tttgcaaaac ataagcaata atcaatgaaa tggaaatcgt
     3121 cataataatg atgggtaacg atgatatacc aactttcatc acttgcatga ggtttagtcc
     3181 aaaacccaat aagacaactg ctgtttgtaa aatatattta gacgtaaaag caataccctt
     3241 attaaacaat tgtcgatttt gataaaagga tccaacaatt attccaatca agatagcaaa
     3301 gactggtgct cctattaaag ggagataaag cccaagcaac caagatggta tagcaataat
     3361 aaagcataat attagtccag gaattttttc cttaaataac atgatagtcc tcctcacatt
     3421 gactattata tcacaagctt taaaacgtgg caggaaaact gaaagaggac cagaatatgt
     3481 tctgatcctc tttcattact aattcacaat tatttagtta ctttcataaa tggatatgat
     3541 ttttcattac ctaaaatcaa ttttgagccc tcttttttat aggctgtttt ttcaccattt
     3601 ttcacaatat taccatagaa cgcatttgcc tttttattga tgtgcgatac tttaataaca
     3661 cttgttaagc gatgtacttt accatccata actggcaatg tcatttcacc agtattagta
     3721 ttatattttc ctccggcagc ttgtgcagca ctttggaaac ttctatcaat ttgttggtca
     3781 atttgtttgt cagtgatttt tgtttcatca aaaatctttt tacctgcttc acttaaatca
     3841 ttgtaagtca aaccttgatc ttttaattgt ttttcaagag ctttatcaat aaaagttttt
     3901 atagcttgat ggaattttac ctcatcaatt tgagcagtga ctgttatctt tgcctcatcg
     3961 tttttgacgt tcatatgcat attagcgccc ttaaccaaat cagctgcact aatccccata
     4021 tccgctgcct gacttccaag ttgttgttct atttctttct caacttgctt ctctaaagaa
     4081 actgtacgcc atttcccctc tagtgactga tgattattcc agaaatagag accgcctgca
     4141 cctac
//
LOCUS       DATRTN010000120         4077 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.120, whole genome shotgun sequence.
ACCESSION   DATRTN010000120 DATRTN010000000
VERSION     DATRTN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.120"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..113)
                     /locus_tag="VBE22_001857"
     CDS             complement(<1..113)
                     /locus_tag="VBE22_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEO7203286.1"
                     /translation="MRKDNLGIILGLSAYVLWGLLSLYWKLLSGIEAYSTFA"
     gene            complement(100..966)
                     /locus_tag="VBE22_001858"
     CDS             complement(100..966)
                     /locus_tag="VBE22_001858"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEO7203287.1"
                     /translation="MRIIVPATSANIGPGFDSIGVALSKYLSIEVLEESTEWLVEHNL
                     VNIPKDHTNLLIQTALHVKSDLAPHRLKMFSDIPLARGLGSSSSVIVAGIELANQLGN
                     LALSQKEKLEIATRLEGHPDNVAPAIFGDLVISSIVKNDIKSLEVMFPDSSFIAFIPN
                     YELKTSDSRNVLPQKLSYEDAVASSSVANVMVASLLKGDLVTAGWAIERDLFHERYRQ
                     PLVKEFGVIKQISTQNGAYATYLSGAGPTVMVLCSKEKEQPIVTELSKLCLDGQIQVL
                     NIERKGVRVEKR"
     gene            complement(968..2251)
                     /locus_tag="VBE22_001859"
     CDS             complement(968..2251)
                     /locus_tag="VBE22_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEO7203288.1"
                     /translation="MTIKIALLGFGTVAKGIPYLLKENQHKLLSLEGEDIVIDKVLVR
                     DNESRQRFINQGFTYNFVTEINTILQDPQIDIVVELMGGIEPAKTYLSQALGFGKHIV
                     TANKDLIALHGKELMDLADARGLALFYEGAVAGGIPILRTLSHSFASDKMTRLLGILN
                     GTSNFMLTKMFEEGWSYEQALKKAQELGYAESDPTNDVEGIDTAYKATILSQFGFGMP
                     IDFDDVNYKGISSIRSEDVEVAQEMGFAIKLVADLRETPTGISVDVSPTLISQEHPLA
                     AVNHVMNAVFIESIGIGQSLFYGPGAGQNPTATSVLADIIDISRSIRSQIKIKPMNTY
                     HCPCRLSMQSDIFNEYYLAISLRNAEDSDTLGRYFEQENIGLKNVIEKALDDKQQEIY
                     VLTDEVSQEKITQFIEEFPESGVIQLINVFKVIGG"
     gene            2428..3315
                     /locus_tag="VBE22_001860"
     CDS             2428..3315
                     /locus_tag="VBE22_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO7203289.1"
                     /translation="MAHTPTSHRKPRKRSPWLAIASVFFLLIALIGIFLFFNNRSKQE
                     IKTKTNASSHRKIVTSIKKKKWVKQKTPVKIPILMYHAVHVMDPSEAASANLIVAPDI
                     FESHIKRLKKEGYYFLAPNEAYRALNENALPEKKVIWITFDDGNADFYTKAYPILKKY
                     KVKATNNIITGFVQEGRESNLNVQQMLEMKQNGMSFQGHTVTHPNLSLLTPELQTQEM
                     TLSKLFLDQKLSQDTLAIAYPSGRYNPTTLDIASQYYKLGLTTNEGVATKDNGLLSLN
                     RVRILPTTSDDDLIKTINQ"
     gene            complement(3338..>4077)
                     /locus_tag="VBE22_001861"
     CDS             complement(3338..>4077)
                     /locus_tag="VBE22_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEO7203290.1"
                     /translation="LGPTVRIFDTLTESLGSYLQNFFGMSFRAAAFDNTKRSWIDNWT
                     IFYWAWWISWSPFVGVFIARISKGRSIREFLTVVLLIPTLLSFVWFAAFGTLSTQVQQ
                     LGINLTKFATEEVLFATFNHYTLGWLLSTIAIILIFSFFITSADSATYVLAMLTEDGN
                     LNPKNRTKVIWGLVLAVIAIVLLLSGGLLALQNVLIIVALPFSFVMILMMLALLVELF
                     HEKKEMGLSISPDRYPRKNEPFKSYEE"
BASE COUNT         1385 a          854 c          609 g         1229 t
ORIGIN      
        1 gcaaacgtac tatatgcttc aattccagat aataatttcc aataaagaga taaaagcccc
       61 cataaaacat aggctgacaa gccaaggata attcctaaat tatcttttct caactctcac
      121 tccttttcgc tcaatattaa gtacttgaat ctgtccatct aaacataatt tacttaactc
      181 tgtgacaatt ggttgttctt tttctttcga acacagaacc attaccgtcg gtcctgctcc
      241 agacaaataa gtagcatacg caccgttttg agtgctgatt tgttttataa ctccaaattc
      301 cttcaccaaa ggttgacgat atctttcatg aaagaggtcc ctttctatag cccatccagc
      361 agttaccaaa tcacctttca acaacgacgc aaccatcaca ttagcgacgc tgcttgaagc
      421 aactgcatcc tcataactca atttttgagg tagtacattt cgactatcac ttgtttttaa
      481 ttcataattt ggaataaaag cgataaagga actatctgga aacatcactt ccaaactttt
      541 aatatcattt ttcactattg atgaaataac taaatcacca aaaatggccg gtgctacatt
      601 atcaggatgt ccttctaatc gggtagctat ctctaatttt tctttttgag aaagagccaa
      661 atttcctaat tgattggcta attctattcc tgcaacaatt acagaggaag aagaacctaa
      721 acctctggcc aaaggaatat ctgaaaacat ttttaagcgg tgtggtgcca aatccgactt
      781 aacatgcaaa gctgtttgaa taaggaggtt agtatgatct tttggaatat ttactaaatt
      841 gtgttcaact aaccactctg tactttcttc taatacttca atgcttaaat actttgataa
      901 cgcaactcct attgaatcaa agcctggacc aatattcgca ctagttgcag gtacaattat
      961 cctcatttca ccctcctatt actttgaata cattgattaa ctgaatgaca ccactctcag
     1021 gaaactcctc aataaattga gttattttct cttggctaac ttcatctgtt aatacataga
     1081 tttcttgttg tttatcatcc aatgcttttt caataacatt tttcaaacct atattttctt
     1141 gctcaaagta ccttccaagt gtatcactat cttcagcatt tctcaaagaa atagctagat
     1201 agtactcatt gaagatatca gactgcattg acaacctaca cggacaatga taagtattca
     1261 taggcttaat ttttatctgt gatcgaatac tacgactaat atcgatgata tcggctaaaa
     1321 cagaggttgc tgttggattt tgtcccgcac ctggtccata aaaaagagac tgaccaatcc
     1381 ctattgattc aatgaatact gcattcatca catgattaac tgcagctaag ggatgctctt
     1441 gagaaattag tgtcggagaa acgtctacac ttataccagt tggagtttca cgaagatcag
     1501 ctaccaactt aatggcaaag cccatctcct gagctacttc aacgtcctct gagcgaatac
     1561 tagaaatccc cttataatta acatcatcaa aatcaatagg cataccaaat ccaaattgac
     1621 ttaagatagt ggctttgtag gcagtatcaa taccttcaac atcatttgta ggatcacttt
     1681 cggcataacc taactcttgt gcctttttta gagcttgttc ataagaccat ccctcttcaa
     1741 acatttttgt taacatgaag ttggaggtac cgttgagaat tcctaataaa cgtgtcattt
     1801 tatctgaggc aaacgaatgc gatagggtcc ttaaaatagg aatgcctcca gcaactgctc
     1861 cctcatagaa taaagctaga cctctagcgt ctgctaaatc catcaactct tttccgtgta
     1921 aagcaatgag atctttattg gctgtcacaa tatgtttacc aaatcctaat gcttgactca
     1981 aataagtttt agctggctca atccccccca ttaattccac tacaatatca atttgtggat
     2041 cttgaagaat agtatttatc tctgtcacaa agttataagt aaatccctga ttgatgaaac
     2101 gctggcggct ttcattatct cttactaata ctttatcaat cacaatatct tcgccttcta
     2161 aagaaagtag cttatgttga ttttctttta gcaaatatgg aataccctta gcaaccgttc
     2221 caaaacctaa taaagctatt ttaatagtca tgaatttctc cttagaaatt gtctctaact
     2281 tcaagacaaa cattggctat attataacaa attaccaata ttttttatag ggtaaacgcg
     2341 tcttgtccta tgaaagttct taaaaagaga atattatttc agaaaagatg atacaataaa
     2401 agtactttaa aagttaagga gaaataaatg gcacacacac ctactagtca tcgtaaacca
     2461 cgaaaacgtt ccccatggtt agctattgct tctgtctttt ttttgttaat agctttaatc
     2521 ggcatatttc tattcttcaa caatcgttca aagcaagaaa taaaaacaaa aacgaacgcc
     2581 tctagtcatc gaaaaattgt cacatctatc aaaaaaaaga aatgggttaa gcaaaagact
     2641 cctgttaaaa tacccatttt aatgtaccat gctgttcatg ttatggaccc ttctgaagcc
     2701 gccagtgcta acttaattgt ggcacctgac atttttgaaa gtcatataaa acgtctcaaa
     2761 aaggaaggat attacttctt agcccctaac gaggcatatc gtgcactaaa cgaaaatgct
     2821 ttacctgaaa aaaaagtcat ttggattact tttgatgatg gaaatgccga tttctacact
     2881 aaggcatacc ctatactcaa aaagtacaag gttaaagcca ctaataatat catcactggc
     2941 tttgttcaag aaggacgaga atctaacctc aacgttcaac aaatgctaga aatgaagcaa
     3001 aacggcatgt cattccaagg acatacggtt actcacccca atctttctct cttaactcca
     3061 gaactacaaa cccaagaaat gacactatca aaactgtttt tagatcaaaa actctctcaa
     3121 gatactcttg cgattgctta tccatcagga cgctacaatc ctacaacttt ggatattgct
     3181 agtcaatatt ataaactagg gcttacaaca aacgaaggag ttgcaacaaa agataacggt
     3241 ttgctttctc ttaatcgagt tcgtatctta ccaactacta gcgatgatga tttgatcaaa
     3301 acgattaacc aataaaaaga agccttctgg cttcttttta ttcttcataa gatttaaatg
     3361 gttcattttt acgtggataa cgatctggag aaatcgataa gcccatttct tttttctcat
     3421 ggaaaagctc cactaatagc gctagcatca tcaaaatcat tacgaatgaa aatggcagag
     3481 cgacaataat taaaacgttc tgcagcgcta acagaccacc agacaagagt aagacaatag
     3541 caatcactgc caacactagc ccccaaatta ctttagttcg attttttgga tttaaattac
     3601 catcttctgt caacatagcc aaaacgtacg ttgcagaatc tgctgatgta ataaaaaatg
     3661 aaaaaattaa aatgatagca atagtggata aaagccaacc taaagtgtag tgattaaaag
     3721 tagcaaacaa tacttcctct gttgcaaact ttgtcaaatt aatacccagt tgttgtacct
     3781 gagttgataa tgtgccaaat gctgcaaacc atacaaaact caataatgtc ggtattaaaa
     3841 gaactaccgt taaaaactcc cgaatgctac gccctttaga aatacgagcg atgaaaactc
     3901 caacaaaagg agaccaggaa atccaccacg cccaataaaa aatcgtccaa ttatcaatcc
     3961 aagaacgttt agtattgtca aaagcagctg cacgaaagct cattccaaag aaattttgta
     4021 aataagagcc taaagactct gttagggtat caaaaatacg aaccgttggt cccaata
//
LOCUS       DATRTN010000121         3996 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.121, whole genome shotgun sequence.
ACCESSION   DATRTN010000121 DATRTN010000000
VERSION     DATRTN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.121"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            143..1288
                     /locus_tag="VBE22_001862"
     CDS             143..1288
                     /locus_tag="VBE22_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7203291.1"
                     /translation="MGQEPIIEYQNINKVYGENVAVEDINLKIYPGDFVCFIGTSGSG
                     KTTLMRMVNHMLKPTNGTLLFKGKDISTINPIELRRRIGYVIQNIGLMPHMTIYENIV
                     LVPKLLKWSEEAKRAKARELIKLVELPEEYLDRYPSELSGGQQQRIGVIRALAADQDI
                     ILMDEPFGALDPITREGIQDLVKSLQEEMGKTIILVTHDMDEALKLATKIIVMDNGKM
                     VQEGTPNDLLHHPATSFVEQMIGEERLLHAQADITPVKQIMLNNPVSITAEKTLTEAI
                     TLMRQKRVDSLLVTDNGKLIGFIDLESLSSKYKKDRLVSDILKHTDFYVMEDDLLRNT
                     AERILKRGLKYAPVVDHENNLKGIVTRASLVDMLYDIIWGDTETEDQ"
     gene            1288..1923
                     /locus_tag="VBE22_001863"
     CDS             1288..1923
                     /locus_tag="VBE22_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7203292.1"
                     /translation="MVNFLSQYGMQILVKTWEQVYISFFAIALGIAIAVPLGVVLTRF
                     PKVAKIIIAIASMLQTIPSLALLALMIPLFGIGKVPAIVALFIYSLLPILRNTYIGMN
                     NVNPTLKDCAKGMGMKPIQSIFQVELPLATPIIMAGIRLSTIYVIAWATLASYIGAGG
                     LGDLIFSGLNLFQPKLILGGTIPVIILSLIIDYLLGLLETALTPRTTRREA"
     gene            1926..2852
                     /locus_tag="VBE22_001864"
     CDS             1926..2852
                     /locus_tag="VBE22_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO7203293.1"
                     /translation="MLKKSHFLQIFTLCLALLTISGCQLTDTKKPGHTTIKVAAQSST
                     ESSIMANIVTELIHHELGYNTTLISNLGSSTVTHQALLRGDADIAATRYTGTDITGTL
                     GLKAVKDPKEASKIVKTEFQKRYNQTWYPTYGFSDTYAFMVTKEFARQNKITKISDLK
                     KLSTTMKAGVDSSWMNREGDGYTDFAKTYGFEFSHIYPMQIGLVYDAVESNKMQSVLG
                     YSTDGRISSYDLEILRDDKKFFPPYEASMVVNNSIIKKDPKLKKLLHRLDGKINLKTM
                     QNLNYMVDDKLLEPSVVAKQFLEKNHYFRGDK"
     gene            2853..3503
                     /locus_tag="VBE22_001865"
     CDS             2853..3503
                     /locus_tag="VBE22_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7203294.1"
                     /translation="MKQMNTFQQFIYYFQHNGSYILEQFIHHFLISIYGVILAAIVGI
                     PLGIFIARRGKIKTFVMGIANVIQTIPSLAMISIIMLGLGLGIKTVVATVFLYSLLPI
                     ITNTYTGIRNVDSDLLDAAKGMGMTKRQRLFMVELPLSISVIMAGLRNALVVAIGITA
                     IGAFVGGGGLGDIIIRGTNATNGGAIILAGSLPTALMAIFSDLILGGIQRMLEPRK"
     gene            complement(3565..3699)
                     /locus_tag="VBE22_001866"
     CDS             complement(3565..3699)
                     /locus_tag="VBE22_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO7203295.1"
                     /translation="MFMPIGTLFMTIFSFALSNVSFIVIGCAIAILGGLGFSYSKKQF
                     "
     gene            complement(3760..>3996)
                     /locus_tag="VBE22_001867"
     CDS             complement(3760..>3996)
                     /locus_tag="VBE22_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO7203296.1"
                     /translation="YATILSISAIGSIIGALIARKIKSSINSMLSMLVFSSLGVIVMG
                     FPSLFELPIWISYSGSFLFNSLLTMFNIHFFSQV"
BASE COUNT         1319 a          707 c          688 g         1282 t
ORIGIN      
        1 ttttttctac attttctgaa aatgtagatc gggcgcaagc ttaacgattc tttttaaaat
       61 cattaaattt taaaacaaat ttcagacatg ttgccaaagt tttgatatta ttactataat
      121 atagtttgta gaggagaata atatgggcca agaacctatc atcgaatatc aaaatatcaa
      181 taaagtgtat ggggaaaatg ttgcggttga agatattaac cttaaaattt accctggtga
      241 tttcgtttgt ttcatcggta cgagtggatc aggtaaaaca acattaatgc gtatggttaa
      301 ccatatgtta aaaccaacaa atggtactct attatttaag ggaaaagata tttctactat
      361 taaccccatt gaattaagac gcagaattgg atatgttatc caaaacattg gtttaatgcc
      421 tcatatgacc atttacgaaa atatagttct tgtaccaaaa ttattgaaat ggtcagaaga
      481 agctaaaaga gctaaagcaa gggaacttat taaattagtt gaattacccg aagaatattt
      541 ggatcgctac cctagtgagt tgtctggcgg tcagcaacaa cgtatcggtg tcattcgcgc
      601 tcttgcagca gaccaagata ttattttaat ggatgagcct tttggagctc tggatcctat
      661 tactagagaa ggtattcaag acttagtcaa gtctcttcag gaagaaatgg ggaaaactat
      721 catcttagtt actcatgata tggatgaagc cctcaagtta gcaacaaaaa ttattgttat
      781 ggacaatggt aaaatggtcc aagaagggac acccaatgat ctcttacatc atcctgctac
      841 cagtttcgtt gaacaaatga ttggggaaga gcgtcttctt catgcgcagg ctgatattac
      901 ccctgttaaa cagataatgt taaataatcc tgtttcaata actgctgaaa aaacactaac
      961 tgaagctatt acactaatgc gccaaaaacg cgttgactca cttctagtaa ccgataacgg
     1021 taaattaatt ggttttattg acttagaatc tctaagcagt aaatataaga aagaccgact
     1081 tgtttctgat atcttaaaac atactgattt ttatgttatg gaagacgact tacttagaaa
     1141 tactgctgag cgtattttaa aacgtggttt aaaatacgct ccagttgttg accatgagaa
     1201 taacctaaag ggcattgtta ctcgtgcatc cctagttgat atgttatacg atattatttg
     1261 gggcgatact gaaacggagg atcaataatg gtcaatttct tatcacagta tggcatgcaa
     1321 atattagtaa aaacatggga acaagtctat atctcatttt ttgctatagc acttgggatt
     1381 gcaattgctg ttccattggg tgtggtctta acacgttttc ccaaagtagc caaaattatc
     1441 attgctatag ctagcatgct acaaactata ccaagtttag ccttattagc tttaatgatt
     1501 ccattgtttg gaattggaaa agtcccagct atcgttgctt tatttattta ttccttgctt
     1561 cctatactaa ggaatactta tataggtatg aataatgtca atccaacact taaagattgt
     1621 gctaaaggga tgggaatgaa acctattcaa tctatatttc aagttgagtt acctttagct
     1681 actccaatta ttatggctgg tattcgcttg tctactattt atgttattgc ttgggctaca
     1741 ttagcttctt atataggtgc aggtggactt ggtgatctca tttttagcgg gttaaatctt
     1801 tttcagccca aattaattct agggggcaca attcctgtta ttatcctgtc acttattata
     1861 gattatcttt taggattact ggaaacagct ctaacgccca gaacaacaag aagggaggct
     1921 tagatatgct taaaaaatcg cactttttac agatatttac actttgctta gccctcttaa
     1981 cgatttctgg ttgtcaatta accgatacta aaaaacctgg tcataccaca attaaggttg
     2041 ctgcccaaag ttctacagag tctagtatca tggcaaatat tgtcaccgaa ttaattcatc
     2101 acgaattagg atacaacaca actttaataa gcaatcttgg ttcctctacg gttactcacc
     2161 aagctttgct ccgtggtgat gctgacattg ctgccacacg ttatacagga acagacatca
     2221 caggaactct tggcttaaaa gctgttaaag accctaaaga agcttctaag attgtaaaaa
     2281 ctgaattcca aaaacgctac aatcaaactt ggtatcctac ttatggtttt tctgatactt
     2341 atgcattcat ggttactaaa gagtttgcca gacagaataa aatcaccaag atctctgatc
     2401 tcaaaaagtt atcaacaact atgaaggcag gggttgatag ttcatggatg aatcgcgagg
     2461 gagatggata cactgatttc gctaaaacat acggttttga attttcacat atttacccta
     2521 tgcaaattgg cttagtctat gatgcagttg aaagtaacaa aatgcaatct gtattaggct
     2581 actccactga cggtcgtatt tcgagctatg atttagaaat tttaagggat gataaaaaat
     2641 tctttcctcc ttatgaagcc tctatggttg tcaacaattc tatcatcaaa aaagatccta
     2701 aactaaaaaa attactccat cgactcgatg gtaaaatcaa tttaaaaacg atgcaaaacc
     2761 ttaattatat ggtagatgat aaacttttag aaccttcagt tgttgccaaa caatttttag
     2821 aaaaaaacca ttattttaga ggagataaat aaatgaaaca aatgaatact ttccaacaat
     2881 ttatctacta cttccaacat aatggtagtt atatcttaga acaatttatc caccactttt
     2941 taatttcaat ttacggcgtt attttagctg caattgttgg tatccctcta ggtatcttca
     3001 tcgctagacg tggtaaaatt aaaacctttg ttatgggaat cgcaaatgtc atccaaacca
     3061 ttccttcttt agctatgatc tctatcatta tgttaggtct agggctaggt attaaaacag
     3121 tagttgcaac tgtctttctt tattctcttc tgccaatcat cacaaatact tacactggta
     3181 ttagaaatgt tgactccgac ttactagatg ctgctaaagg aatgggcatg actaagcgtc
     3241 aacgtttatt tatggttgag ttaccactat ctatttctgt tatcatggct ggacttagaa
     3301 atgcccttgt tgtagcaatt ggtattacag ccattggtgc tttcgtcggt ggaggcggac
     3361 ttggagatat catcataaga ggaactaatg caacaaatgg tggtgctatt atcttagcag
     3421 gatctcttcc aacagccctt atggcaattt tctctgattt aatcctaggc ggtatccaac
     3481 gcatgcttga accaagaaaa taactattaa aaacttgtca gtagagacaa gtttttttga
     3541 tgtttcagaa taatttctta agttctaaaa ttgtttttta ctatagctaa atcctaatcc
     3601 tcccaatata gcaatggcac atcctataac aataaagcta acatttgata aggcaaatga
     3661 aaagatagtc ataaatagtg ttcctatagg cataaacata atagcaatag taaatatagt
     3721 cgacatcaca cgccccatat acgcttcatc gaccctgatt tatacctgac tgaaaaaatg
     3781 aatattaaac attgtcagaa gactattaaa aagaaaacta ccactgtaag atatccatat
     3841 cggtaattca aatagagatg ggaatcccat aactatgact cccaaactac tgaatactag
     3901 catagatagc atagaattaa tactagattt aatttttcta gcaataagcg ctccaataat
     3961 tgatccgata gctgatatac taagtattgt tgcata
//
LOCUS       DATRTN010000122         3940 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.122, whole genome shotgun sequence.
ACCESSION   DATRTN010000122 DATRTN010000000
VERSION     DATRTN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.122"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(92..1519)
                     /locus_tag="VBE22_001868"
     CDS             complement(92..1519)
                     /locus_tag="VBE22_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO7203297.1"
                     /translation="MTKEYQNYVNGEWKSSVNQIEILSPIDDSSLGFVPAMTREEVDH
                     AMKAGREALPAWAALTVYERAQYLHKAADIIERDKEEIATVLAKEISKAYNASVTEVV
                     RTADLIRYAAEEGIRLSTSADEGGKMDASTGHKLAVIRRQPVGIVLAIAPYNYPVNLS
                     GSKIAPALIGGNVVMFKPPTQGSVSGLVLAKAFAEAGLPAGVFNTITGRGSEIGDYIV
                     EHEEVNFINFTGSTPVGQRIGKLAGMRPIMLELGGKDAGIVLADADLDNAAKQIVAGA
                     YDYSGQRCTAIKRVLVVEEVANELAEKISENVAKLSVGDPFDNATVTPVIDDNSADFI
                     ESLVVDARQKGAKELNEFKRDGRLLTPGLFDHVTLDMKLAWEEPFGPILPIIRVKDAD
                     EAVAIANKSDFGLQSSVFTRDFQKAFDIANKLEVGTVHINNKTGRGPDNFPFLGLKGS
                     GAGVQGIRYSIEAMTNVKSIVFDMK"
     gene            complement(1669..3402)
                     /gene="ptsP"
                     /locus_tag="VBE22_001869"
     CDS             complement(1669..3402)
                     /gene="ptsP"
                     /locus_tag="VBE22_001869"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEO7203298.1"
                     /translation="MTEMLKGIAASDGVAVARAYLLVQPDLSFETVTVEDTNAEEARL
                     DVALQASQDELSVIREKAVESLGEEAAAVFDAHLMVLSDPEMINQIKETIRAKQVNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITERVQDGQ
                     LIAVNGITGEVIIEPTEAQISAFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNENGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAIFEEEKANLLADGVAVAEGIEVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALSECSTMEEV
                     IELQKEYVDFD"
     gene            complement(3407..3670)
                     /locus_tag="VBE22_001870"
     CDS             complement(3407..3670)
                     /locus_tag="VBE22_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEO7203299.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAIEETMTKEGLA"
BASE COUNT         1175 a          823 c          666 g         1276 t
ORIGIN      
        1 aaccacttta taagattata taattcttta tttttaatgt aaaagaggtt tataaaacga
       61 ctcaactgac aatagttgag tcgttttacg tctatttcat atcaaaaaca atcgatttta
      121 catttgtcat tgcttcaatt gaatatctga taccttgaac acctgcacca gatcctttga
      181 gtcctaagaa tgggaagtta tcaggaccac gtccagtctt attgttaatg tgaactgtac
      241 caacttcaag tttatttgct atatcaaatg ctttttggaa atcacgtgta aagactgatg
      301 attgtaatcc aaaatcagat ttgttggcaa tagcaacagc ttcatctgca tccttgacac
      361 gaataattgg gagaattggt ccaaaaggct cttcccaagc tagtttcata tctaaagtaa
      421 catgatcaaa caatcctgga gttaatagac gaccatcacg tttaaattca ttcaattctt
      481 tcgcaccttt ttgacgtgca tctactacta agctttcaat aaagtcagct gaattatcat
      541 caataaccgg cgtcactgtt gcattatcaa atggatcacc tactgataat tttgctacat
      601 tttcagatat tttttctgcc aattcatttg caacttcttc aacgacaagc acacgcttaa
      661 ttgccgtaca gcgttgtcca gagtaatcat aagcacctgc aacgatttgt ttagcagcgt
      721 tgtcaaggtc agcatcagct aagacgatac ctgcatcctt accgcccaac tcaagcataa
      781 ttggacgcat tcctgccaac ttaccaatac gttgtccaac tggcgttgat cctgtaaagt
      841 taataaaatt aacttcttca tgctcaacaa tgtaatctcc aatctcagaa ccgcgtcctg
      901 taatagtatt aaagacacct gctggaagac ctgcttctgc aaaagctttt gctaaaacaa
      961 gtcctgagac tgaaccttgt gttggtggtt taaacatcac aacgtttcca ccaattagag
     1021 ctggcgcaat ttttgatcct gagaggttaa cagggtaatt ataaggtgcg attgctaaaa
     1081 cgatacctac tggttgacga cgaataacag ccaacttatg acctgttgaa gcatccattt
     1141 ttccaccttc gtcagctgaa gttgataaac gaattccttc ttctgctgca taacgaataa
     1201 gatcagctgt ccttacaacc tcagttactg aagcattgta agctttagaa atttcttttg
     1261 ctaaaacagt agcaatttct tctttatcac gttcaataat gtctgcggct ttatgaaggt
     1321 attgtgcacg ttcatatact gtcaaagcag cccaggctgg taaagcttca cgacccgctt
     1381 tcatggcatg atcaacttct tctcgagtca tcgctggcac gaatcccaat gaagaatcat
     1441 caattggtga caaaatctca atctgattaa cagatgattt ccattcgcca ttgacataat
     1501 tttgatattc ttttgtcaaa gtaaatctcc ttttacattg taataggttt attctatcac
     1561 tttatctatt tatttcaatt atctgacata tttttcttag aaacttaaca atatccaaac
     1621 tttcaataaa aaagtagaga ttttttaatc cctacttttt tattattctc agtcaaagtc
     1681 aacgtattct ttttgaagtt cgataacttc ttccatagtt gaacactctg aaagagcacg
     1741 attagcgtat tcttccattt tagctgtgtc caattttttc attaagctac gtgtacgaag
     1801 tactgaggtt gctgacattg agaattcatc caatcccata ccaacaagaa gtggaaccgc
     1861 agtttgatca ccggccatct caccacacat acctgcccat ttaccttcag catgagcagc
     1921 tttgataacg ttgttaatca aacgaaggat tgatggattg tatggttgat agaggtatga
     1981 aacttgctca ttcatacggt ccgcagccat tgtgtattgg ataagatcat tagtaccaat
     2041 tgagaagaaa tcaacttctt ttgcaaattg gtctgcaagc atagctgccg ctgggatttc
     2101 aatcataata ccaacttcaa tgccttcagc tactgctaca ccgtcagcta acaagttcgc
     2161 tttctcttct tcaaatatag ctttagcagc acggaattct ttaagcaaag caaccattgg
     2221 gaacatgatg cgtagctgtc catgaactga agcacgaaga agtgctcgga tttgtgtgcg
     2281 gaacattgca tcaccagttt cagaaattga aatacgcaat gcacggaatc ctaagaatgg
     2341 gttcatctct tttggaagat caaagtaagg aagctcttta tctccaccga tatccattgt
     2401 acgaacaaca actggtttac cattcatacc ctcaagaact gctttatatg cttcatattg
     2461 ctcatcttct gttgggaaat cttgtgaatc catatataag aactctgtac ggtaaagacc
     2521 aactgcttct gctccatttt cgttaacacc ctcaacatct ttaggtgtac cgatattagc
     2581 agctaattca aaatgtttac catcagcagt aactgtttgt gcatccttaa gaagtgccca
     2641 ttcagctttt tgcttggcat aagcttcacc tgctgccttg aaggctgaaa tttgagcttc
     2701 agttggttca atgataactt caccagtaat accattaaca gcaatcaact gaccatcttg
     2761 aacacgttca gtaatatcat ttgtaccaag aacagctgcg atttcgagtg tacgtgccat
     2821 aatagctgag tgacttgtac gccctccaat atttgttaca aaagctttaa caaattgttt
     2881 atttaattga gctgtatcag aaggtgtcaa atcgtgtgca ataacgattg attcttcatt
     2941 gatagttgct gggtttggta atttaacacc caaaagatgt gctaagacac gtttggcaac
     3001 atcacggata tctgctgcac gttcttgcat gtatgggtta tcttccatac cttcaaagat
     3061 agtgatgaac atatcagtca cttctttaag acctgtttct gcatttactt gttttgcacg
     3121 aatagtctct ttaatttgat taatcatttc tgggtcagaa agaaccatta aatgagcatc
     3181 aaatacagct gcggcttctt cgcctaagct ttctactgcc ttctcacgga taacagaaag
     3241 ctcgtcttgt gaagcttgga gtgcaacatc aaggcgagct tcttctgcgt ttgtatcttc
     3301 gactgttaca gtctcaaatg acaaatccgg ttggacaagt agatatgctc tagcaactgc
     3361 aacaccatca gaggctgcga ttcctttaag catttctgtc attttcttat gccaatcctt
     3421 cttttgtcat tgtttcttcg atagctgcaa ttgcatcatc tgcatctgca ccttctgctg
     3481 aaatagtaac atcagcacct tgaccaacac caagactcat aacacccatg attgatttaa
     3541 ggtttactgc tttacctttg taatcaagcg tgatgtctga agcaaatttg ctagctgttt
     3601 ggacaagcaa agtagctgga cgtgcgtgaa tacctgtttc tgcaacaatg tggaaatctt
     3661 ttgaagccat attatggttc tccttttgtc atttgtaaat atttgagtca cctattggta
     3721 acccttacaa gttaaaagta taacatattg ctttttattt ttcaagatta aatgtctcaa
     3781 acagaaaaaa acttgaatgt tttccacagc ttttcatatt aaaaatgaga gtcttagaat
     3841 ttaaaaatag aattctaaaa atgatttttt tatgttcttc tgaaaggaat tggaaaacag
     3901 gctgaaagct tttaaactaa ccctaaaaac gaaatatagt
//
LOCUS       DATRTN010000123         3912 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.123, whole genome shotgun sequence.
ACCESSION   DATRTN010000123 DATRTN010000000
VERSION     DATRTN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.123"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            305..1072
                     /gene="cfb"
                     /locus_tag="VBE22_001871"
     CDS             305..1072
                     /gene="cfb"
                     /locus_tag="VBE22_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfb"
                     /protein_id="HEO7203300.1"
                     /translation="MNVKHMMYLSGTLVAGALLFSPAVLEVHADQVTTPQVVNHVNSN
                     NQAQQMAQKLDQDSIQLRNIKDNVQGTDYEKTVNEAITSVEKLKTSLRANPETVYDLN
                     SIGSRVEALTDVIEAITFSTQHLANKVSQANIDMGFGITKLVIRILDPFASVDSIKAQ
                     VNDVKALEQKVLTYPDLKPTDRATIYTKSKLDKEIWNTRFTRDKKVLNVKEFKVYNTL
                     NKAITHAVGVQLNPNVTVQQVDQEIVTLQAALQTALK"
     gene            1245..1547
                     /locus_tag="VBE22_001872"
     CDS             1245..1547
                     /locus_tag="VBE22_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO7203301.1"
                     /translation="MTKKITVVALETLIAQHNNIHLIDVREEHEYRGGHISGAINLPL
                     SQLSHKFEQLDKNKEYYLVCQRGGRSIRACQFLELKGYKVINVDGGTEAWRGNLEV"
     gene            complement(1704..2567)
                     /locus_tag="VBE22_001873"
     CDS             complement(1704..2567)
                     /locus_tag="VBE22_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO7203302.1"
                     /translation="MNQEKKRLNLIGLILLAQAIVLSLATTLFAEILQNDVWIGIAST
                     LIALLIPCFFMGFKNLKKHLTATHHTITFTTILFLLSIIYTTNNIVSTIWDVVLNHLN
                     IMESESQNYDATFNILMISYSVVVGPFFEEVLYRGIVLNLLSKYGKWFAIITSGILFG
                     LMHQDISQLLTTSIAGIIMGFIAYHYSFKVALLLHICNNFIVEIFTQLSTVNELYGTY
                     FENILLILAILFILYYLFTHRNTARRRISLHSNVREADSINSKQHTKLLLTSWPFILL
                     VIYDIVLTTIN"
     gene            complement(2872..3432)
                     /locus_tag="VBE22_001874"
     CDS             complement(2872..3432)
                     /locus_tag="VBE22_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO7203303.1"
                     /translation="MRITIFCGASTGENPVYSEKTVALAQWMAQNKHSLVYGGGKVGL
                     MGVMADTVIANGGYTTGVIPTFLRDREIAHENLSELIIVNNMPERKAKMMLLGDAFIA
                     LPGGPGTLEEISEVISWSRIGQNDNPCILYNVNGYFNDLKNMFDHMVGEGFLSLEDRE
                     NVLFSDDITEIEDFITNYKVPSTRQY"
     gene            complement(3449..3745)
                     /locus_tag="VBE22_001875"
     CDS             complement(3449..3745)
                     /locus_tag="VBE22_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO7203304.1"
                     /translation="MDITDYQKWVSEFYKKRNWYQYNSFIRSNFLSEEVGELAQAIRK
                     YEIGRDRPDETEQTDLENLNDIKEELGDVLDNIFILADQYNISLEEIISAHRTN"
BASE COUNT         1380 a          586 c          686 g         1260 t
ORIGIN      
        1 gataagtaga aattttctaa aatatttaga tttatataag gtggtaaatt atcgttatgg
       61 ttttttacat gaaaatctaa attaaattat cagaaattaa caaaaggata ggcgcaagta
      121 tattatagca aaatgataat attagtttta tgaaaaaaat gcagtttatt tgtaataatg
      181 taaaatatag gggaaaagaa agcgcttgac gaccttttgg acaagtagta agatacaaac
      241 atgggccctg taaattaaaa atactgcagt aaaagtgatt ttagtttaaa ggaggaaatt
      301 cattatgaac gttaaacata tgatgtatct atctggaact ctagtggctg gtgcattgtt
      361 attttcacca gctgtattag aagtacatgc tgatcaagtg acaactccac aagtggtaaa
      421 tcatgtaaac agtaataatc aagcccagca aatggctcaa aagcttgatc aagatagcat
      481 tcagttgaga aatatcaaag ataatgttca gggaacagat tatgaaaaaa cggttaatga
      541 ggctattact agtgttgaaa aattaaagac ttcattgcgt gccaaccctg agacagttta
      601 tgatttgaat tctattggta gtcgtgtaga agccttaaca gatgtgattg aagcaatcac
      661 tttttcaact caacatttag caaataaggt tagtcaagca aatattgata tgggatttgg
      721 gataactaag ctggttattc gcattttaga tccatttgct tcagttgatt caattaaagc
      781 tcaagttaac gatgtaaagg cattagaaca aaaggtttta acttatcctg atttaaaacc
      841 aactgataga gctaccatct atacaaaatc aaaacttgat aaggaaatct ggaatacacg
      901 ctttactaga gataaaaaag tacttaacgt caaagaattt aaagtttaca atactttaaa
      961 taaagcaatc acacatgctg ttggagttca gttgaatcca aatgttacgg tacaacaagt
     1021 tgatcaagag attgtaacat tacaagcagc acttcaaaca gcattaaaat aatatttgta
     1081 tttttcgtgt gatgctgtcg acttcgtgat tttgtactac catggttttt atgattaaaa
     1141 ggtttacgat aatagtcata atagtagaac gatgtcacca ttttaaataa taaagtgatt
     1201 agtcatttga ctaaatttgc caagtatcaa aggaaataaa gattatgact aaaaagataa
     1261 ctgttgtagc attagaaaca ttgattgccc agcataataa tatccatttg atagacgttc
     1321 gtgaagagca tgagtatcgt ggggggcata tttcaggtgc gataaatctt cctttgagtc
     1381 aactcagtca taagtttgaa cagttagata aaaataagga atattatctt gtttgtcaac
     1441 gagggggaag atctattaga gcatgccaat ttcttgagtt gaaaggctac aaagtaatca
     1501 atgtagatgg cggcacagaa gcgtggagag gaaatctaga agtgtaaata tgtcctgtgt
     1561 agtctaccat atttgataat aaaaaaattg acattatctc actattaatg accttttagt
     1621 aaacataaag aaaagtatgt gttttgaaaa ggaggatgat tatccttttc aaaaccatac
     1681 tttttgtgtc ttgtggcttg gtcttaatta atagttgtta ggacaatgtc gtaaatgaca
     1741 aggagaataa aaggccaact agtaagtaat aattttgtat gttgtttaga gtttattgag
     1801 tcagcctctc gaacgttaga gtgcagactt atacgacggc gagcagtatt cctatgagta
     1861 aaaaggtaat ataatataaa taagattgct aaaattagca atatattttc aaaataagtc
     1921 ccgtataact cgtttactgt tgatagctgg gtgaaaattt ctacgataaa gttattgcag
     1981 atatgaagga gtagggctac cttaaaggaa taatggtaag cgataaaacc cattatgata
     2041 cccgcaatag atgtagtcaa tagctgagaa atgtcttggt gcattaaacc aaataggatg
     2101 ccagaagtga taatggcaaa ccatttacca tacttggata ataaattcaa tactattcct
     2161 cggtataaaa cttcttcaaa aaaaggacct acaacaacag aataactaat cataagtata
     2221 ttgaatgtgg catcataatt ttgactttcc gattccataa tattgagatg atttagcaca
     2281 acatcccata ttgtggagac tatattatta gtagtatata taatgcttaa caagaataaa
     2341 atagtggtga aagtgattgt atgatgggtt gccgttagat gtttttttaa atttttaaaa
     2401 cccataaaaa aacaaggaat gagtaaggcg attaaagtac ttgctatacc tatccagaca
     2461 tcattttgta agatttcagc aaatagtgta gtcgccaaac ttaaaacaat agcctgagct
     2521 aaaagtatca gcccgattag gttaagtctc tttttttctt gattcaattg attacctcct
     2581 tattaataac ggtaacgaca aagaaatatt ataatgtata atgtataggt ttctaagtca
     2641 ttagtcaata gggacgagca cttggatact tctaataaga taaaatacat agacaagact
     2701 atagttttgg cttctctaaa gattacctaa aaatttataa gttctcgagc agaaaggatg
     2761 agtttggata ttgagaggat gggcttgacc agctgaaaga aaaatgaacc aagggtaatc
     2821 gcttcgaaaa aagaaggaaa tttcaattag aaacttcctt tttttatcaa tctagtattg
     2881 gcgcgtgcta gggactttat aattcgttat aaaatcttcg atttcagtaa tatcatctga
     2941 gaataggaca ttttctctat cttccagact aagaaaaccc tcaccaacca tatgatcaaa
     3001 catatttttt aaatcattaa agtagccatt aacattatat aaaatgcaag gattatcatt
     3061 ttgaccgatg cgagaccaag agataacttc ggaaatttct tcaagggtac caggtccacc
     3121 aggtagagcg ataaaggcat ctccaagtag catcattttt gccttacgtt caggcatatt
     3181 atttactatg ataagctctg ataaattttc atgtgcaatt tcacgatctc ttaaaaatgt
     3241 cggaataaca ccagtagtgt agccaccatt tgcaatcaca gtatcagcca tgactcccat
     3301 taagccaact tttcctccac catacaccag agaatgtttg ttttgtgcca tccattgtgc
     3361 taaggcaact gttttttcac tataaacagg attttcccca gtacttgctc cacaaaaaat
     3421 agttattctc atgtttcaaa cctcttttct aattagtgcg atgtgcgctg ataatctctt
     3481 ctagtgagat attatactga tcagcaagta tgaagatatt atctaagaca tctcctagtt
     3541 cttcttttat atcatttaag ttttccaaat cagtttgttc ggtttcgtca ggtctgtcac
     3601 gtccaatttc atatttgcga atggcttgag ccaattcgcc gacttcttct gaaagaaagt
     3661 tacttctaat aaaagaattg tattgatacc agtttctttt tttatagaat tcactcaccc
     3721 atttttgata atctgtaata tccattattc cctccgtttc aagtatattg tagcaatatt
     3781 gctttattaa tgaaaatttg aacgctaatt aataggaaaa gttcgtattt ggtatttctt
     3841 ctagtttgtg aatagtcata tttaaaccta aaaggttagc gcgtacttta agcatttttt
     3901 aaatatttta aa
//
LOCUS       DATRTN010000124         3890 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.125, whole genome shotgun sequence.
ACCESSION   DATRTN010000124 DATRTN010000000
VERSION     DATRTN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.125"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            131..331
                     /locus_tag="VBE22_001876"
     CDS             131..331
                     /locus_tag="VBE22_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO7203305.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFVEKTIAKGK
                     ELADDAKDAVEEAVDAVKEKLK"
     gene            complement(372..542)
                     /locus_tag="VBE22_001877"
     CDS             complement(372..542)
                     /locus_tag="VBE22_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203306.1"
                     /translation="MDKELTPQEKANKKWAENNREHRTYLSKRSTARSFINKNATKED
                     LLELKQLIESKL"
     gene            959..1138
                     /locus_tag="VBE22_001878"
     CDS             959..1138
                     /locus_tag="VBE22_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000965641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO7203307.1"
                     /translation="MLYIDEFKEAIDKGYISGNTVAIVRKNGKIFDYVLPHEEVRDDE
                     VVTVESVEEVLRELK"
     gene            complement(1296..2110)
                     /locus_tag="VBE22_001879"
                     /pseudo
     CDS             complement(1296..2110)
                     /locus_tag="VBE22_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015968524.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            2110..2514
                     /locus_tag="VBE22_001880"
     CDS             2110..2514
                     /locus_tag="VBE22_001880"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO7203308.1"
                     /translation="MIYSTLAKEQGVQGYLDGKGSLRDICKWYDISSRSVLQKWIKRY
                     TSGEDLKATSRGYSRMKQGRQATFEERVEIVNYTIAHGKDYQAAIEKFGVSYQQIYSW
                     VRKLEKNGSQGLVDRRVKGLESRPDLTEIEQL"
     gene            2676..2876
                     /locus_tag="VBE22_001881"
     CDS             2676..2876
                     /locus_tag="VBE22_001881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7203309.1"
                     /translation="MPIQALCQLLKGSRSGYYKWLNRQKTDFETKNTKLMAKIKELRR
                     LYNGILGYRRMTTFINRQLGTT"
     gene            2898..3158
                     /locus_tag="VBE22_001882"
     CDS             2898..3158
                     /locus_tag="VBE22_001882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7203310.1"
                     /translation="MMNILGISSVIRRVSHACTKAGDRFYEENILNREFTATAHNQKW
                     CTDVTYLQYGLGAKAYLSAIKDLYNGSIIAYEISHNNEIHLL"
     gene            3284..3493
                     /locus_tag="VBE22_001883"
     CDS             3284..3493
                     /locus_tag="VBE22_001883"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO7203311.1"
                     /translation="MSRIGKCIDNAPTESFFGFFKTESYHLKKYNSYDELVNDVARYI
                     EFYNTQRYQSKLNNLTPLEFRNQVA"
     gene            complement(3666..3827)
                     /locus_tag="VBE22_001884"
     CDS             complement(3666..3827)
                     /locus_tag="VBE22_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEO7203312.1"
                     /translation="MGGIGAHKFYAGKIGQVFLYIIFSITFIPGIIDLIEALMALGKQ
                     TDEYGNIIV"
BASE COUNT         1269 a          619 c          726 g         1276 t
ORIGIN      
        1 agtaggcaga ttacagtata ggtgcatatt atttagcaag aatcgcctga cactagcggt
       61 tcttgctttt ttatttgcct agaaataatc aaaatgttac catagaataa aaataataag
      121 gaggcacatt atgtcacaag aaaaactaaa atcaaaatta gatcaagcaa aaggtggtgc
      181 taaagaaggc tttggcaaaa taaccggtga taaagagtta gaagcaaaag gatttgttga
      241 aaaaacaatt gctaaaggca aagaactagc agatgatgct aaagatgctg ttgaagaggc
      301 agtagatgct gtcaaagaaa aactgaaata aatattaacc gctctctatt gagggcggtt
      361 tttttgtgtg tctagagttt gctttcaatt aattgtttta attctaataa gtcttctttt
      421 gtagcatttt tgttaataaa actacgagca gtagatcgtt ttgatagata ggttctatgt
      481 tctctattgt tttctgccca ctttttattt gctttttctt gaggtgttaa ttctttatcc
      541 atttcaatca tccttgttaa taaagtaaaa tacaactaaa caaattgcga aaataatcaa
      601 atatttcata tttgtcttag atatgatata ctatcagtag tggcaagggg cttgagcccc
      661 aaactactac tagaacctta tttgaatctc cgtggccggt ttttcttttt aggttctttt
      721 tttattgctg tgattatgct tgctatacca accaacagag ttccgattga agtaagcaaa
      781 tcagcaattt ctgatattct catatcttcc tcctttctat atataattat agtacatgta
      841 ctatataaag tcaatacttt tttttaaaat atttatcttt ttgtctatca gaacagaaaa
      901 atttaaaatt gtctattttt aggatttttt atcgaataga tagataggag gataaaatat
      961 gttatatata gatgagttta aagaagcgat tgataaaggc tatatttcag ggaacacagt
     1021 agcgatagtg cgtaaaaacg gaaagatatt tgattatgtg ttgccgcacg aggaagtgag
     1081 agatgatgaa gttgtgacag ttgagagtgt agaagaggtg ttgagggaat tgaagtgaaa
     1141 gggtgcaaaa aaggggcaaa atagattaaa aatgaaacta cattgaactt tttactatta
     1201 taaacaacca ttaaatcaac gcttggacta ttatgaacta ttgtgtacaa tagtagtaga
     1261 ttccggcagg ggacatattc attgcatgta aatagcggtt tagagctatt ttgccccaaa
     1321 tttctctgat taagtttatc gttcctatct ttttgttctt gtaattgatg tgcgtaaact
     1381 tctaaagtga tatttaaatt ctcgtgatct aaaacttgag agatggaaat tagatagctt
     1441 gcaaatgtat gcctgagaga gtgcactcgt acctcgcgac cagttatttt tcggatagtt
     1501 ttattgactg cattatttga aagtttgtcg aataatctgt cgtttttatt ttttgtaaat
     1561 tcatgcaaaa aaaataatgt atcattgtca attggtatat ttctgatact acttttgttt
     1621 tttgttggca ggtatctttg gttgaaatga taatcccaag ttttattaat tgataaatat
     1681 ttgttagtgt aatcaatatc attaactgtt aaacctaaac attcagcgaa gcgcatgcca
     1741 gttttagcga tgaggtataa cgctgcatac gattgatgtt gtgatttttc tttacaaatt
     1801 tttatcaagc gtaagtattc attggtttca agaaatttta tctctattta cgccccttat
     1861 tttttgcttt aaccttagtg aataaacaaa aatttttttc tatatatccc tcgtgaacag
     1921 ccatggatac gcaggctttt acatgtatgt taaaacgctt tactgtatct tgcacatgcg
     1981 tttgactata atgatttatg acttgttgat atttagtgga agtaatattg caaagtaata
     2041 tatttcctat tatatgttta tacgatattc gatattccca cccgttgtcg cgtttacgga
     2101 aatacgccat tgatatactc cacattagct aaagaacagg gtgttcaagg ctaccttgat
     2161 ggaaaaggct ctcttagaga tatttgtaaa tggtatgata tctcaagtcg ctctgttctc
     2221 caaaagtgga taaaacggta tactagtggt gaagacttga aagccactag tagaggatat
     2281 agccgtatga aacaaggaag gcaagccaca tttgaagaac gtgtagagat tgttaactac
     2341 accattgccc atgggaaaga ctatcaagca gctattgaga agtttggtgt ttcctaccaa
     2401 caaatttatt cttgggtgcg taagcttgag aagaatggct cacaaggttt ggttgataga
     2461 cgtgtgaaag ggttggagag taggcctgat ttaaccgaga ttgagcaact ttaactcaag
     2521 attaaacaat tggaggaacg taatcgtctc ttagaaatcg aggttagttt actaaaaaag
     2581 ttagaagaca tcaaacgagg aaacagacgg taagactagg taagcattta gcggagttcc
     2641 aagtaatcaa gaattattac gatgaggaat ctaatgtgcc tattcaggcc ttatgccaac
     2701 tcttgaaggg gtctcgttca ggctattaca agtggctcaa tcgtcaaaaa acagattttg
     2761 agacaaaaaa tacaaagcta atggctaaaa tcaaggaact tcgtagactc tacaatggta
     2821 tcttaggtta tcgccgtatg acaacattta ttaatcgtca acttgggaca acttaaaaca
     2881 agaaacggat tcgttgattg atgaacattc tggggattag ttcagtcatt cgtcgtgtta
     2941 gccatgcttg tacaaaagct ggtgacagat tttacgaaga aaatattctt aatcgtgaat
     3001 ttacagccac agctcataac cagaaatggt gcacagatgt cacctatctt caatacggtc
     3061 tgggagctaa agcttatctc agtgcgatta aagacctgta taacggttct attatcgctt
     3121 atgagattag tcacaacaat gaaatccact tgttatgaag accattaaaa aggggctaga
     3181 gctcaatcca ggagccacac ctatcatcca tagcgattga ggtagtcaat atacttccaa
     3241 agaataccgt tatatcatac aacaagctgg tctgacctta tccatgtccc ggattggcaa
     3301 atgtattgat aatgcaccaa ctgaaagttt ctttgggttt ttcaagactg agtcttacca
     3361 ccttaagaaa tacaactctt atgatgagtt ggtcaatgat gtggcacgtt atatcgaatt
     3421 ctacaacaca caacgttatc aatcaaaatt aaacaacctg actcctctag aattcaggaa
     3481 tcaggttgca taacttatct tttattattt gactgtctac ttgacaggga gccgttcaga
     3541 ttgcttaacc tttctaaatt tgctaaaata gctacaagaa aacgagccat ttaatgctta
     3601 tttcttatac tgtcttgcct cacgctctcc tcgaccaaaa attgagcgtg aggctttttg
     3661 tttcattaaa cgatgatatt tccatattca tcagtttgtt ttccgagagc catcaaagct
     3721 tcgataaggt cgataattcc aggaataaag gtaatactaa aaataatata taaaaaaacc
     3781 tggcctattt ttcctgcgta aaatttatgc gctccaatgc cgcccaaaag aacgttaata
     3841 aaacataaac tactatgtta gcataagact ttatttttac aactgaattt
//
LOCUS       DATRTN010000125         3804 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.126, whole genome shotgun sequence.
ACCESSION   DATRTN010000125 DATRTN010000000
VERSION     DATRTN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.126"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(139..2436)
                     /locus_tag="VBE22_001885"
     CDS             complement(139..2436)
                     /locus_tag="VBE22_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO7203313.1"
                     /translation="MFKGNKKLNSSKLGDYTPLEFGSIFLRIVKLLSDFIYVIILLFV
                     MLGVGLAVGYLASQVDSVKVPSKNSLVTQVNTLTRVSRLTYSDKSQISEIDTDLQRTP
                     VAKDAISDNIKKAIIATEDENFNDHKGVVPKAVLRAAAGSVLGFGESSGGSTLTQQLL
                     KQQILGDDPSFKRKSKEIIYALALERYMDKDSILSDYLNVSPFGRNNKGQNIAGIEEA
                     AQGIFGVSAKDLTIPQAAFLAGLPQSPIVYSPYTADAQLKSDKDLSFGIKRQKNVLYN
                     MYRTRALTKDEYKSYKDYDIKKDFIKPAVATTNHHDYLYYSALSEAQKVMYNYLIKKD
                     NVSEHDLKNDETRATYRHRAIEEIQQGGYTIKTTINKSVYQAMQDAAAQYGGLLDDGT
                     GKVQMGNVLTDNSSGAIIGFIGGRNYSENQNNHAFDTARSPGSSIKPILPYGIAIDQG
                     MLGSGSVLSNYPTTYSSGEKIMHADEEGTAMVNLQESLDISWNIPAFWTYKMLRDRGV
                     DVKNYMEKLDYPIENFGIESLPLGGGIDTSVAQQTNLYQMIANGGVYHKQYMIESIED
                     SNGKVIYNHESKPVRVFSKATATILQQLLHGPINSGKTTTFKNRLQGLNSGLAGVDWI
                     GKTGTTNSTSDVWLMLSTPKVTLGGWAGHDNNASLAKLTGYNNNANYMAHLVNAINNA
                     DGNTFGKSERFRLDDSVIKAKVLKSTGLQPGVVTVNGRRITVGGESTTSYWAKNGPGT
                     MTYRFAIGGTDSDYQKAWSTLGGKR"
     gene            2547..>3804
                     /locus_tag="VBE22_001886"
     CDS             2547..>3804
                     /locus_tag="VBE22_001886"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEO7203314.1"
                     /translation="MNIFDELKERGLVFQTTDEDALRKALEEGSVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDVERSLQTKETVVSWGNKIR
                     GQLSNFLEFETGDNKAVLVNNYDWFSNISFIDFLRDVGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFYELNKNYNVTLQIGGSDQWGNMTAGTELIRRKSNGVSHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDADAVRFLKIFTFLSLKE
                     IEDIRIQFEEAPHQRLAQKTLAREVVTLVHGEKAYKEAVNITEQLFAGNIKGLSVKEL
                     KQGLRGVPNYHVQTEDNLNIIDLLVTSGVVNSKRQAREDVSNGAIYINGDRIQDLEYT
                     ISENDKLENEITVIRRGKKKYFVLNFK"
BASE COUNT         1200 a          733 c          636 g         1235 t
ORIGIN      
        1 taaacatagc atactggtgt ttttctaatt ctttgaaaga agactataca agaattaaat
       61 catattatct tggaaaaaat aaaaggagaa cagatcactc tattctcctc aaataaaact
      121 atcagtatcg taaacctgtt atcgttttcc acccaaagta gaccaagctt tttgataatc
      181 actatcagtt cccccaatag caaaacgata ggtcatcgtt ccaggcccat tttttgccca
      241 ataacttgtt gtgctttcac ctccaacagt gattcgacga ccgtttactg ttactacacc
      301 tggctgtaaa cctgtagact taagaacttt agcttttata acgctatcat caagtctaaa
      361 cctttctgac ttaccaaaag tattgccatc tgcattatta atagcattaa ccaagtgagc
      421 catataatta gcattattat tgtaacctgt caatttcgct aaagaggcat tattatcatg
      481 tcctgcccat ccacctaaag tcactttagg tgtagaaagc atcaaccaaa catcagaagt
      541 tgagttagta gtacccgttt ttccaatcca atctacccca gctaaaccac tatttaaacc
      601 ttgtaagcga tttttaaagg ttgtagtctt tccggagtta attggtccat gtaatagctg
      661 ctgtaagata gtagctgttg ctttcgaaaa gacacggaca ggtttacttt cgtgattata
      721 aatcactttc ccattactat cctctatact ttctatcata tactgcttat gataaacacc
      781 accatttgca atcatttgat aaaggtttgt ttgctgagca actgacgtat caataccacc
      841 accaagtggt aaactttcta taccaaagtt ttcaattgga tagtctaatt tctccatata
      901 gttttttaca tcaacgcctc gatctcgtag catcttgtaa gtccagaaag ctggaatgtt
      961 ccaagaaatg tccaatgact cttgaaggtt aaccatagca gtaccttctt catccgcatg
     1021 cataatcttt tcgccacttg aatatgtcgt tggataattt gaaagaacac taccgctgcc
     1081 taacatccct tgatcaatag caattccgta aggtaatatc ggtttaatac ttgaccctgg
     1141 agagcgtgcc gtatcaaaag catggttatt ttgattctca ctatagttac gaccaccaat
     1201 aaatcctata atagcaccac tagaattgtc cgttagtaca tttcccattt gaaccttgcc
     1261 agtgccatca tctaacaacc ctccgtattg agctgcagca tcctgcatgg cttgataaac
     1321 agatttattg atagtagttt taatagtata ccctccctgt tgaatctctt caatagcgcg
     1381 atgtctgtac gtcgctctag tctcgtcatt ttttaaatca tgctctgaaa cattatcctt
     1441 tttaatgagg taattgtaca tcactttttg agcttcagat aatgccgagt agtagagata
     1501 atcatgatga tttgttgtcg ccactgcagg tttgataaaa tcttttttga tgtcataatc
     1561 tttgtaggat ttgtactcat ctttggttaa agctctcgtt ctatacatat tgtataacac
     1621 atttttttgg cgctttatac caaaactaag atctttatct gatttcaact gggcatctgc
     1681 agtataagga gaatatacga taggactttg cggtaaacct gcaaggaaag ctgcctgagg
     1741 aattgttaaa tcttttgcgg aaactccaaa aatcccttgt gctgcttctt caatacctgc
     1801 aatattctgt cctttgttat tgcgaccaaa cggtgataca tttaaataat ctgataaaat
     1861 cgaatcctta tccatgtaac gctctaatgc tagagcataa ataatttctt ttgatttgcg
     1921 tttaaatgac ggatcatctc ccaaaatttg ctgctttaat aattgttgtg tcagcgttga
     1981 accaccgcta ctctctccaa accccagaac agatcctgct gcagcacgta gtacggcttt
     2041 gggaactaca cctttatggt cattgaaatt ttcgtcctct gttgcaatga tagccttttt
     2101 aatattatca gatatagcat cctttgccac tggtgtacgt tgtaaatcag tgtcaatctc
     2161 tgaaatttgt gatttatcag aataagttaa cctagacacc ctagtaagtg tattaacttg
     2221 tgtcactaaa ctatttttac taggtacttt aacagagtca acttgactag caagataccc
     2281 tactgcaagt cctactccga gcataacaaa tagtaaaata ataacataga tgaaatctga
     2341 taaaagcttt acaattctta aaaaaataga accaaattca agtggtgtgt aatcacctaa
     2401 tttagaacta ttcaacttct tattaccttt aaacatgaat aatacctcat cctacattat
     2461 atcagattta atataacttg catatcctaa agagttttga tatagttgac attgatacac
     2521 aataagttaa taattaggag aaaaagatga atatttttga tgaactcaaa gaacgtggac
     2581 tcgtttttca gacaactgac gaagatgctt tacgcaaagc tcttgaagaa ggttctgtct
     2641 cttactatac tggctacgat cctacggctg atagccttca tcttggacat ttagttgcta
     2701 ttttaacctc tcgtcgctta caacttgctg gtcacaagcc ttatgcactt gtgggaggcg
     2761 caactggact tattggggat ccttcattta aagatgttga gcgcagttta caaacaaagg
     2821 aaactgtagt tagttggggt aataaaatcc gcggtcaatt atctaatttt ctagagtttg
     2881 aaacaggtga taacaaagct gtattggtaa ataattatga ttggttcagc aatattagtt
     2941 tcattgattt cttacgagat gttggtaaat attttactgt caattatatg atgagtaaag
     3001 aatctgttaa aaaacgtatc gaaacaggga tttcctatac tgaatttgct tatcaaatta
     3061 tgcaaggata tgacttttac gaattaaata agaactataa tgtcactttg caaattggtg
     3121 gttctgacca atggggcaac atgactgctg gaaccgaact tattagacga aaatcaaatg
     3181 gtgtatctca tgttatgact gttccattga taacagattc tacaggaaaa aaattcggaa
     3241 aatctgaagg aaatgctgtt tggcttgatg ctgacaaaac ctctccttat gaaatgtacc
     3301 aattttggct caatgttatg gatgctgatg ccgttcgctt ccttaagatt tttacattcc
     3361 taagccttaa agaaatagaa gatatacgta ttcaattcga ggaagcacct caccaacgct
     3421 tagcacaaaa aacactagca cgtgaagttg taactcttgt tcatggtgaa aaagcttata
     3481 aggaggccgt taatatcacg gaacagctat ttgctggcaa tataaaagga ttgtctgtta
     3541 aagagctcaa acaaggttta cgtggagtac caaattatca cgttcagacg gaagataacc
     3601 tcaatatcat agaccttcta gtcacttcag gagttgtcaa ttctaaacgt caagctcgag
     3661 aagatgtctc caatggagct atttatatta atggagatcg tattcaagat ttagaatata
     3721 ctatctcaga aaatgataag cttgaaaatg aaattacagt cattcgtcgt ggaaagaaaa
     3781 aatactttgt attaaacttt aaat
//
LOCUS       DATRTN010000126         3490 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.127, whole genome shotgun sequence.
ACCESSION   DATRTN010000126 DATRTN010000000
VERSION     DATRTN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.127"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            174..1553
                     /locus_tag="VBE22_001887"
     CDS             174..1553
                     /locus_tag="VBE22_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7203315.1"
                     /translation="MIELKGVNFKYKNTKNSCLDNIDLEIKKGEFVLISGASGSGKTS
                     ITRVINKLIPYYYEGELEGTVKINNDLVSSYQMFELSEIVGSVFQNPRTQFFNVDTNS
                     EIVFGLENQGVSRDILKKRLKETCETLDINKLQNRNIFHLSGGEKQKIAFASVYAMNP
                     DIYLLDEPSSNLDIPTIEVLKRHLSVLKNSGKTVIISEHRIYYLMDLVDKVIYMKNGQ
                     IQKSYSKEDFLNLSEDEIKAMALRCRKKTKLNIEMSNNEKHPYLQVNDFSIYRKDNCI
                     INNMNFSANKGDIVAVIGSNGVGKTTFLRTLCGLHDNYKGDVLLNSKKTDAKKRRQKN
                     YMVMQDVNYQLFAESVFAECKLGIENVSDDLIEEILNEFDLSDYKNNHPNTLSGGQKQ
                     RLAIAVGLICKKEIFVLDEPTSGLDYTNMVKTVELLKRYSKDKIIFIATHDTEFANLI
                     CNRVLDLEK"
     gene            1574..3330
                     /locus_tag="VBE22_001888"
                     /pseudo
     CDS             1574..3330
                     /locus_tag="VBE22_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382833.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         1278 a          436 c          666 g         1110 t
ORIGIN      
        1 tattatggct tctaaagcag cagacgaatt aactatcgct tctattacaa gaggaattga
       61 atctcctaaa aaacgaagtt caattattga aattcgtttt gatattcaag acgttttagc
      121 tttactgatt ggagtggtaa ttactgtatt tgtgatatat aggaggtttt tctgtgattg
      181 aattaaaagg ggttaatttc aaatataaaa acaccaaaaa cagctgctta gacaatatag
      241 atttggaaat aaaaaaggga gaattcgtct taatttccgg tgcttcaggt tctggaaaaa
      301 catctataac gagagttata aataagctga ttccttatta ttacgaaggt gaattagaag
      361 gcacagtaaa aataaacaac gatttagtat ctagctacca aatgtttgag ctttcagaaa
      421 tagtaggtag tgtttttcag aatcctagaa cacaattttt caatgtggac acaaatagtg
      481 aaatagtttt tggtttagaa aatcaagggg tttctagaga cattttaaaa aaaagattaa
      541 aagagacttg tgagacgttg gatataaaca aattgcagaa taggaatatc tttcatctgt
      601 caggagggga aaagcagaag attgcttttg cgtcggtgta tgcaatgaat cccgatattt
      661 atttactgga tgaaccctca tctaatttgg atataccaac gatagaggtg ctaaaaagac
      721 atttgtctgt tttaaaaaac agtggcaaaa cggttattat atctgaacat cgaatctact
      781 atttgatgga tttggttgac aaagttatat atatgaaaaa tggacaaatt caaaaaagtt
      841 attcaaaaga agattttttg aatttgtctg aagatgaaat taaagctatg gctttaagat
      901 gcagaaaaaa aacaaaactg aatatagaaa tgtctaataa tgaaaaacat ccatatttac
      961 aagtgaatga tttttctata tacaggaaag ataattgcat tataaataac atgaactttt
     1021 ctgcgaacaa aggcgatatt gttgcggtta taggttcgaa tggtgttggt aaaacaacat
     1081 ttttaagaac tctttgtggt ctgcatgaca actataaagg tgatgtgttg ttgaattcta
     1141 aaaaaacaga tgcaaaaaaa agaagacaaa aaaactatat ggttatgcaa gatgtgaatt
     1201 atcaattatt tgccgagagt gtctttgctg aatgtaaatt aggaatagaa aatgtgagcg
     1261 atgatcttat agaagaaata ctaaatgaat ttgatttgag cgactataaa aataatcatc
     1321 caaacacctt atccggagga caaaaacaaa gattggcaat agccgtagga ctaatatgca
     1381 aaaaagaaat ttttgtgtta gatgaaccaa ctagtggact tgactatacg aatatggtaa
     1441 agactgtaga attgttaaaa cgttattcca aagacaaaat tatttttatt gctacgcatg
     1501 ataccgaatt tgctaatttg atttgtaatc gggtattgga cttagaaaaa tagatggaaa
     1561 ggaggacttt aatgtggacg ttttaagaag attatataag ttcgcacctg aaaaaaaggt
     1621 gtactcctat gcgtcgctag ttttatctgc aatagctact atattctcag tagtcccata
     1681 tttttattta tggaagtttt tgaatgaact tttagtactt aaaaaccttg aaagtgcaaa
     1741 taaatatgcg ttatggattt ttatgttttt ggttttacaa acattagtgt attttttgag
     1801 tttatattgt agccacatgt ttgcttttag agtcgaaaga aatttaaaaa tagaaggctt
     1861 aaacaacctt ttagaggcat ctttttcttt ttttgataca aatccatcag gaaaaactag
     1921 gcaaataata gacgataatg cgtcggaaac acattccata cttgctcaca tgcaaccgga
     1981 catggtcaat gctatggttt atcctgtaat tttgctggtt gtaagttttt tggtaagtat
     2041 aagttttggg atagtaatgg ttgtatcatt agttttaggc gtatttttaa tcacgaaaat
     2101 gtttgggaat caaaattttc tccaaaaata tatggcttcc aatgaagata tgagttccga
     2161 aatagttgaa tatataagag gtgtcaaggt tatcaaaata ttcaatatag gaatagaaaa
     2221 atttcgttct ttgcataaaa caatcatcac ctcttcagaa ctaggttatg cgtacgctat
     2281 ctcgtgtaga aaatggaagg ttttttacga ttcatttttc atggggctat gcatggttac
     2341 gataccttat ggtgtatatt tgctaaacat taatacgcat ctaggagaaa ttatttccta
     2401 caccgtattt tcacagcatt tattgaattg ttatacaacg cttttaataa agtgatgttt
     2461 ttgggacaaa ataaggctaa agccaaaaac tctatagata agctggaaga aatttttaac
     2521 aaaatgcagg taaaaaaatt agatagtggg ctttcagatg aaatacaaaa ttttgatatt
     2581 gaatttgaaa aagtatcgtt taaatacgaa ggaaatacac catatgtttt aaaagactta
     2641 tcgtttaagt tggattcggg aaaaagttat gcacttattg gttcatctgg cagtggaaaa
     2701 tccacaattg ctaagttaat ttccgggttt tatgatgttg attccggagt aataaaaatt
     2761 ggtggacgag atattaaaga gtattcaagg gaaacgatag caagtagtat cgcttttgtt
     2821 ttccagaatg cgaaattatt taaaatgagt atttatgaca acgtgagaat tggaaatcct
     2881 gatgctactt atgaagagat tatgcaagct cttgaaaagg ctaggtgcaa tgaaattttg
     2941 gataagtttg agctgaggga aaatacaatg atcggagcag aaggcgttta tctctcagga
     3001 ggagaaactc aaagaatagt aattgcaaga gcaattctaa aaaattcacc cattataata
     3061 cttgatgagg caagtgcagc tgctgatccg gaaaatgaat atgaactcca gatggcattt
     3121 acgcaattga taaaaaataa aacagtaata atgattgctc acaggctgtc agcaattaaa
     3181 aacatggacg aaatcatagt tgtagaaaat ggacagatta tagaaagagg aagtagcaaa
     3241 cttttattag aggataaaag ctctaaatac agctatttta tgaatatgta tattcgagca
     3301 aatgattgga taatgtctaa tgaagtctaa attaatggat aaatacggaa taacagaaga
     3361 cagctcaata gatctaatta aagcttcgat agcgtcgttc tggaaaaatg tagtatacat
     3421 gttccctata acgcttatat tattttttct catgaatata agcaactcga aatatctaca
     3481 agagtggttt
//
LOCUS       DATRTN010000127         3477 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.128, whole genome shotgun sequence.
ACCESSION   DATRTN010000127 DATRTN010000000
VERSION     DATRTN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.128"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            128..838
                     /locus_tag="VBE22_001889"
     CDS             128..838
                     /locus_tag="VBE22_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7203316.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYETATAFDGREALVQYAEF
                     QPDLIILDLMLPELDGLEVAKEVRKTSHIPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTENIETAVAEESAQNASSDITIGELQILPDAFIAKKRGEEIE
                     LTHREFELLHHLATHIGQVMTREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPGR
                     PEYILTRRGVGYYMKSYE"
     gene            831..2180
                     /gene="vicK"
                     /locus_tag="VBE22_001890"
     CDS             831..2180
                     /gene="vicK"
                     /locus_tag="VBE22_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEO7203317.1"
                     /translation="MNNSAANIRSFELALLFLLVFVAVYFVYLAVRDFKMSKNIRLLN
                     WKVRDLIAGNYSDSILIQGDADLVELGESLNDLSDVFRMAHDNLEQEKNRLASILTYM
                     TDGVLATDRSGKIVMINETAQQQFNLAYDEALSMNIVDMLGSGSPYSFQDLVSKTPEV
                     VLNRRDENGEFVTLRIRFALNRRESGFISGLVAVSHDATEQEKEERERRLFVSNVSHE
                     LRTPLTSVKSYLEALDEGALNEEVAPSFIKVSLDETNRMMRMISDLLSLSRIDNEVTH
                     LDVEMTNFTAFMTSILNRFDQIRNQKTVTGKVYEIVRDYPLKSIWVEIDTDKMTQVID
                     NILNNAVKYSPDGGKITVNLRTTKTQMILSISDQGLGIPKKDLPLIFDRFYRVDKARS
                     RQQGGTGLGLSIAKEIVKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVTYEEWEDVED"
     gene            2184..2993
                     /locus_tag="VBE22_001891"
     CDS             2184..2993
                     /locus_tag="VBE22_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO7203318.1"
                     /translation="MSEIGFKYSILASGSTGNCFYIETPQKRLLIDAGLTGKKVTSLL
                     AEINRKPEDLDAILVTHEHSDHIKGVGVLARKYHLDIYANEQTWKVMDERNMLGKVDV
                     SQKHVFGRGKTLTFGDLDIESFGVSHDAVDPQFYRMMKDDKSFVMLTDTGYVSDRMAS
                     LIENADGYLIESNHDIEILRSGSYPWTLKQRILSDKGHLSNEDGSETMIRTIGNRTKH
                     IYLGHLSKENNIKELAHMTMENNLMRADFGVGTDFSVHDTSPDSATPLTRI"
     gene            2996..3364
                     /locus_tag="VBE22_001892"
     CDS             2996..3364
                     /locus_tag="VBE22_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEO7203319.1"
                     /translation="MKKIFYISLGFISLGIGIAGIVLPVVPTTPLVLLSAFCFSRSSE
                     KFDIWLRQTKVYKYYAADFVESRSIAPARKKSMIWQIYILMGISIYFAPLMWLKLGLL
                     IGTIVGTYVLFYVVPDKIDE"
BASE COUNT         1109 a          502 c          733 g         1133 t
ORIGIN      
        1 aactaggaaa tctgttataa ttagtgataa tagtggtgct ttaggcccat attatcatat
       61 tttcggtcac ttttattttt cagtttatga atggaagtgg gagaccagaa agtaatagag
      121 gtaaaatatg aaaaaaatac ttatcgttga tgatgaaaaa ccaatttcag atattatcaa
      181 atttaattta accaaagaag gatatgagac ggcgacagct ttcgatggcc gtgaagcttt
      241 agtacaatat gcagaatttc aaccagattt aatcattttg gatttaatgc tccctgaact
      301 agatggttta gaagtggcta aggaagttcg taaaaccagc catattccta ttattatgct
      361 ttctgcaaaa gatagtgagt ttgacaaagt cattggttta gaaatcggtg cagatgatta
      421 tgttactaaa cctttttcaa atcgtgaact tttggcacgg gtcaaagcac atttacgacg
      481 tactgaaaat attgaaacag ctgtagcaga agaaagtgct caaaatgctt cttcggatat
      541 tactattggt gagttgcaaa ttttaccaga cgcctttatt gctaagaaac gtggcgaaga
      601 aattgaatta acgcaccgag aatttgaatt acttcaccat ttagcgacac atatcggaca
      661 agtcatgaca cgcgaacatt tattggaaac agtttgggga tatgattatt ttggagatgt
      721 ccgtacagtt gatgtgacgg tgagacgttt acgtgaaaag attgaagata ctcctggtcg
      781 tccagaatat attttgactc gacgtggtgt aggttattac atgaagtctt atgaataata
      841 gtgctgcaaa tattaggtcg tttgagctag ccttactttt tttactagtt tttgttgctg
      901 tttattttgt ttatctagcg gttagagatt ttaaaatgtc taaaaatatc cgacttttga
      961 attggaaagt cagagatttg atagcaggaa attattcaga tagtattcta attcaaggtg
     1021 atgcagattt ggtagaatta ggtgaaagtc taaatgattt atctgatgtc ttccgaatgg
     1081 cacatgataa cttagaacaa gaaaagaatc gcttagcaag tatcttaact tatatgactg
     1141 acggtgtgct ggctacagat cgttcaggaa aaatcgttat gattaatgag acagcccaac
     1201 agcagttcaa tctagcatat gatgaagctt tatcaatgaa tattgttgat atgttagggt
     1261 cgggaagtcc atattcgttc caagatttag tctctaagac gccagaggta gttcttaata
     1321 gacgtgatga aaatggtgaa tttgttacct tacggattcg atttgctttg aaccgtcgtg
     1381 aaagtggttt tatttccggt ttagttgcag tatcacacga tgctactgag caagagaaag
     1441 aagagcgtga aagaagactt tttgtatcta atgtttctca tgaacttcgt acacctttga
     1501 catctgtaaa atcttattta gaagctttag atgagggagc gctcaatgaa gaagttgctc
     1561 caagctttat taaggtatct ctagatgaaa caaatcgtat gatgcgtatg atatcagatt
     1621 tattaagttt atcgcgcatt gataatgaag taacgcattt agatgttgaa atgacgaatt
     1681 ttacagcttt catgacctca attttgaatc gatttgatca gattagaaat caaaaaacag
     1741 tcacaggaaa agtttatgaa attgtcagag attatcctct taagtcaatt tgggtggaaa
     1801 ttgatacaga taagatgact caagtgattg ataacatttt aaataatgca gtcaagtatt
     1861 caccagatgg tggtaagatt acagttaatc tacgcacaac taaaacgcag atgattttat
     1921 caatatcaga ccaaggctta ggtattccca aaaaagattt acctctcatt tttgatcgtt
     1981 tttatcgtgt tgataaggcg agaagtcgtc aacagggtgg gactggactt ggtttgtcaa
     2041 ttgcaaaaga aattgttaag cagcataagg gatttatttg ggctaagagt gagtatggta
     2101 aaggatctac ttttacaatc gtcttgcctt atgataaaga tgctgtaact tatgaagaat
     2161 gggaggacgt tgaagattaa aacatgtcag aaataggatt taaatatagt atcttagctt
     2221 caggttcaac tggaaattgt ttttatattg agacacccca aaaacgtctt ttaattgacg
     2281 ctggtttaac aggcaagaaa gtgactagcc tccttgcaga aattaatcgt aaaccagaag
     2341 atttagatgc tatactagtt acccacgaac attctgacca cattaaaggt gttggcgtat
     2401 tggctaggaa atatcacctt gatatttatg ccaatgagca gacgtggaaa gttatggatg
     2461 agcgtaacat gttaggcaaa gttgatgtta gtcaaaagca tgtttttgga cgcggtaaaa
     2521 ccttaacttt cggggattta gatattgaga gctttggtgt aagtcatgat gctgtggatc
     2581 cccaatttta ccgtatgatg aaagatgata agagctttgt gatgttgaca gatacaggat
     2641 atgttagcga ccgaatggca agtttgattg agaatgcaga tggttatttg atcgagtcaa
     2701 atcacgacat tgagattcta cgttcgggtt cttatccttg gacacttaag caacgtattt
     2761 tatcagataa aggacattta tctaatgaag atggttcaga aaccatgatt cgcactatag
     2821 gtaaccgtac taaacacatc tatttaggac acttgagtaa ggaaaataat attaaggaac
     2881 ttgctcacat gactatggaa aacaatttaa tgagagctga ctttggtgtg ggcacagatt
     2941 tttctgttca cgatacttca cctgattcag ctaccccact gacgaggatt taagattgaa
     3001 aaagattttt tacattagtt taggatttat tagtttaggg ataggcattg caggtattgt
     3061 cttgccggta gttccaacga ctccattagt attgctttca gctttttgct tttcgagaag
     3121 ctctgaaaaa tttgatattt ggttgcgtca aactaaagtt tataaatact acgctgcaga
     3181 ttttgttgaa agcagaagta ttgccccagc gcgtaaaaag tcaatgattt ggcaaattta
     3241 tatcttgatg gggatttcaa tctattttgc tcctctaatg tggctcaaac tgggattatt
     3301 gattggaact attgtaggaa cttatgtgct tttttatgta gtacctgata aaattgatga
     3361 atagttaaca agagactagg tgtgtatcct agtcttttgt tgaatattca aatatgtaat
     3421 taaggaaacg ttttctttgg aagataagtt taacttccaa aatgtttgat tatttgt
//
LOCUS       DATRTN010000128         3337 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.129, whole genome shotgun sequence.
ACCESSION   DATRTN010000128 DATRTN010000000
VERSION     DATRTN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.129"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..525
                     /locus_tag="VBE22_001893"
     CDS             <1..525
                     /locus_tag="VBE22_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7203320.1"
                     /translation="KTFKAYLDLNSQEINSISKNDIIEVDGKYVGNISTKQSFLKEGY
                     QMFGGLLFTGFLLGISFLLGIALIVYYKQYSEGHEDKRSYRILQEVGMSKKLVKRTIN
                     SQIMIFFFQPLVVAVIHFGVAIPMLKQMLLVFGVLNSTIVYVVSGLTVLAISIIYFII
                     YRITSRTYYHIIER"
     gene            635..1303
                     /locus_tag="VBE22_001894"
     CDS             635..1303
                     /locus_tag="VBE22_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7203321.1"
                     /translation="MSQEQGKIYIVEDDMTIVSLLKDHLSASYHVSSVSNFRDVKQEI
                     IAFQPDLILMDITLPYFNGFYWTAELRKFLTIPIIFISSSNDEMDMVMALNMGGDDFI
                     SKPFSLAVLDAKLTAILRRSQQFIQQELTFGGFTLTREGLLSSQDKEVILSPTENKIL
                     SILLMHPKQVVSKESLLEKLWENDSFIDQNTLNVNMTRLRKKIVPIGFDYIHTVRGVG
                     YLLQ"
     gene            1300..2238
                     /locus_tag="VBE22_001895"
     CDS             1300..2238
                     /locus_tag="VBE22_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7203322.1"
                     /translation="MIRQFLREHLIWYILYIMMFVLFFISFYLYHLPMPYLFNSLGLN
                     VIVLLGISIWQYSRYRKKMLHLKYFNSSQDPSFELQPSDYAYFNIITQLEAREAQKVS
                     ETIEQTNHVALMIKMWSHQMKVPLAAISLMAQTNHLDPKEVEQQLLKLQHYLETLLAF
                     LKFRQYRDDFRFEAVSLREVVVEIIKSYKVICLSKSLSIIIEGDNIWKTDKKWLTFAL
                     SQVLDNAIKYSNPESKIIISIGEESIRIQDYGIGILEEDIPRLFEDGFTGYNGHEHQK
                     ATGMGLYMTKEVLSSLNLSISVDSKINYGTAVSIHK"
     gene            complement(2281..>3337)
                     /gene="xerS"
                     /locus_tag="VBE22_001896"
     CDS             complement(2281..>3337)
                     /gene="xerS"
                     /locus_tag="VBE22_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEO7203323.1"
                     /translation="LLEKIDELKEIMPWYVLEYYQSKLSVPYSFTTLYEYLKEYRRFL
                     EWLLDSGVANCHHIAEIELSVLENLTKKDMEAFILYLRERPLLNANTRQNGVSQTTIN
                     RTLSALSSLFKYLTEEVENADGEPYFYRNVMKKVSTKKKKETLASRAENIKQKLFLGN
                     ETIEFLEYIDCEYQNKLSKRALAFFNKNKERDLAIIALLLASGVRLSEAVNLDLKDIN
                     LNVMVIDVTRKGGKRDSVNVASFAKPYLANYLDIRKNRYKAENQDIALFLSEYRGVPN
                     RIDASSVEKMVAKYSQDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHASTQVTD
                     LYTHIVNDEQKNALDKL"
BASE COUNT         1100 a          506 c          586 g         1145 t
ORIGIN      
        1 aaaaccttta aggcatattt ggatttgaat agtcaagaga taaattcaat ttctaaaaac
       61 gacataatag aggtagatgg taagtatgtt ggaaatatta gcactaaaca gagttttcta
      121 aaagaaggtt atcaaatgtt tggtgggtta ttatttactg gatttctgtt aggtataagc
      181 tttttgctag gtatagctct aattgtttac tataagcaat actcagaagg tcatgaagat
      241 aagagatctt atcgtatctt acaagaagta ggtatgagca aaaaattagt taaacggact
      301 attaattctc aaattatgat tttcttcttc caacctttag tggtagcagt tattcacttt
      361 ggagttgcta ttccaatgct aaaacagatg cttttggtat ttggggtgct taactcaaca
      421 atcgtatatg tagtaagtgg tttgacagta cttgcgattt ctataattta ctttatcatt
      481 taccgtataa ctagtcgaac ttattatcat attattgaac gctaaaaagg attggttatt
      541 ttaccaatcc ttttgcatta atagaaaccg atagactaaa aaatttgtta tactaaatga
      601 aagaaagaga tttcgtacta ttttaggagg ggatatgtca caagagcaag gaaaaattta
      661 tattgtagaa gatgatatga cgattgtgtc acttttaaaa gatcatttat cagctagcta
      721 tcatgtctct agtgtcagca attttcgtga tgtgaaacaa gaaattatcg catttcaacc
      781 cgatttgata ctaatggata ttacgttacc ctattttaat ggtttttact ggactgcaga
      841 attgcgtaag tttttaacaa ttcctattat tttcatttca tctagtaatg atgaaatgga
      901 tatggttatg gcattaaata tgggggggga tgactttatt tcaaaaccat tctctctagc
      961 tgtattagat gctaagctaa ctgctatttt aaggagaagt caacaattta tccaacagga
     1021 attaactttt gggggattta cgttgacaag agaagggtta ttgtctagcc aagataaaga
     1081 ggttatttta tcgccaacag aaaataaaat cctatctatc ttgctcatgc atcctaaaca
     1141 agtagtctca aaagagtctc tattagagaa actttgggaa aatgatagtt ttattgatca
     1201 aaatacactt aatgttaata tgacacgctt acgtaaaaaa attgtcccaa taggttttga
     1261 ttacattcat acagtgagag gagttgggta tttactacaa tgatacgcca gtttttaaga
     1321 gaacacttga tttggtatat tttatatatc atgatgtttg tcctattttt tattagtttc
     1381 tatctatatc atttaccaat gccctatttg tttaattcct taggtttaaa tgttattgtt
     1441 ttactaggaa ttagtatttg gcaatacagt cgttacagga aaaaaatgtt acatctcaaa
     1501 tattttaata gtagtcagga cccctctttc gaacttcaac cgagtgatta cgcttatttt
     1561 aatattatta cacaattaga agctagagaa gcgcaaaaag tttctgaaac aattgaacaa
     1621 accaatcatg ttgcacttat gataaagatg tggtcgcacc aaatgaaagt tccattggca
     1681 gctatttcat taatggccca gacaaatcat ctcgatccta aggaagttga acaacaatta
     1741 ttgaaattgc aacattatct tgaaacgttg ttagcatttt tgaaatttag acaatatcgt
     1801 gacgattttc gttttgaagc tgttagcctt agagaagtag tagtagaaat tataaaatcg
     1861 tataaggtta tttgtctatc caaaagctta tctatcataa ttgaaggcga taatatctgg
     1921 aaaacagaca aaaagtggtt aacttttgct ctttcacagg tgctagataa tgccataaaa
     1981 tattctaatc ctgagtcaaa gataataata agcataggag aagagagtat tagaatacaa
     2041 gactacggta tcggcatact cgaagaagat atccctagac tttttgaaga tggctttacg
     2101 ggttacaacg gtcatgagca ccaaaaggca acaggcatgg ggttatatat gacaaaagaa
     2161 gtcttatcta gtctgaattt gtccatttcg gtggatagca aaattaatta tgggactgcc
     2221 gtttctatac ataaataaag ttatgtaaaa taaaatattt acataacttt atttatgtta
     2281 ctatagttta tctaaagcgt tcttttgttc atcgttaaca atatgggtat ataggtcagt
     2341 aacttgagtg gaagcatgac ctaattggtg gctaactaat acttgagatt ttgtagcatc
     2401 atatagccta gtagcaagag tatgccttag tttatgaggt gttacacgaa ccttaaaatc
     2461 ctgggaatat ttagcgacca ttttctcaac acttgaagca tcaatacgat ttggcacccc
     2521 acggtattct gataaaaata gtgcaatgtc ctgattttct gctttgtatc tatttttacg
     2581 gatgtctaaa taattagcaa ggtagggctt cgcaaagcta gcgacattga cagagtccct
     2641 tttcccccct tttcgtgtga catcaataac cattacatta aggttaatat cctttaaatc
     2701 taagtttact gcctcagata gacgcacccc tgaagctagt agcaaagcaa taatagctaa
     2761 atctctctct ttatttttgt tgaaaaaagc taaagcacgt ttggaaagtt tgttttgata
     2821 ttcacaatct atatactcta agaattctat cgtttcattg cccaaaaata acttttgttt
     2881 aatattttct gcccgagaag ctaaagtttc ctttttcttt ttggttgaga cttttttcat
     2941 gacattgcga taaaagtaag gctccccgtc agcattttca acctcttcag taaggtattt
     3001 gaaaagacta gataaggcag atagagttct gttaatggtg gtttgagaca ccccattttg
     3061 ccttgtgtta gcatttaata aagggcgttc tcgtaaatat agtataaaag cttccatatc
     3121 ttttttagta aggttttcta aaactgaaag ctcaatttct gcaatgtgat gacaattagc
     3181 aacaccagaa tccaataacc attcaagaaa acgtctatat tcttttaaat attcgtagag
     3241 cgttgtgaaa ctataaggaa cagaaagctt tgattgatag tattctagaa cataccaagg
     3301 cataatttct tttagttcat cgattttttc gagtagt
//
LOCUS       DATRTN010000129         3235 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.130, whole genome shotgun sequence.
ACCESSION   DATRTN010000129 DATRTN010000000
VERSION     DATRTN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.130"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(30..1178)
                     /gene="nagA"
                     /locus_tag="VBE22_001897"
     CDS             complement(30..1178)
                     /gene="nagA"
                     /locus_tag="VBE22_001897"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEO7203324.1"
                     /translation="MTKYIKADRFFYADHVKENGYLEIKDNHFGKWIENISGQEEILD
                     YSGYQIAPGLVDTHIHGFAGADVMDCDSEGILRMSAGLLSTGVTSFLPTTLTSDTKRL
                     EEASKSVAAVAGKEQGAKIQGIYFEGPYFTEEYKGAQNPIYMRNPNLEEFAQWQKAAK
                     GLITKIALAPEREGVEEFVSAITKQGVTVALGHSNGTYKEAKKAVKAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVDPVACDILMTQKGHNHVALITDCMAA
                     GGAPDGDYMLGELPVVVSNGTARLKDGGNLAGSILKLKDGLKNVVEWGIATPEEAVRM
                     ASYVPAVSVGIDNVCGQIKEGYAADFIVLDKHLDLVATYLDGEKRWQS"
     gene            complement(1456..2163)
                     /locus_tag="VBE22_001898"
     CDS             complement(1456..2163)
                     /locus_tag="VBE22_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEO7203325.1"
                     /translation="MKIDKKEFLALIASIILLIFASVTFFLFKDHGTTQMDTVESSVN
                     HVSDSQLTEAQDMLDKFEKKPSEKLLKDVELALNKLSNSSKKEALQKRFKKAKDKYLK
                     DEADKKATKDATDLVEILEQAPSEENVLKAEAAVNKLTVKESKEALQKRIDTVKTQYG
                     LIGNQTPSSSVAETTEQGTANPASQDTSSYVNQNVAPTYEQPQTNNTPVTPGVNNTVP
                     TPGTGTAPATNGTGVAQ"
     gene            2355..2666
                     /locus_tag="VBE22_001899"
     CDS             2355..2666
                     /locus_tag="VBE22_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO7203326.1"
                     /translation="MIPSQMLKGLLEGAILKIIQQEETYAYEISKKLNSYGFGEISEG
                     TIYPIILRLQSNKYIKGQQKPSPQGPKRKYYTLTNEGVEQLVQFKQNWESLSKALNKL
                     L"
     gene            2687..>3235
                     /locus_tag="VBE22_001900"
     CDS             2687..>3235
                     /locus_tag="VBE22_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203327.1"
                     /translation="MDKLKKLQKENTLLEGRIDNSNNQTYTDMIVYLRGASISPYHQE
                     LIRNDIVNMLLEAQERQASLVSVFGEDRHDFINQVIKSTPKISKKEETLQRWDLAILL
                     LTIQMIIFLGGYLITEALQQSVPDLIPITLLDVLFAIFISIIAVKIADTIIYATYNFD
                     KSKEKKYFFRYMFLILSLIIAYI"
BASE COUNT         1031 a          655 c          486 g         1063 t
ORIGIN      
        1 ttaaaaagaa ctagtaaaaa ctagttcttt caagattgcc aacgtttttc tccatcaagg
       61 tatgttgcaa ctaaatctag atgtttatct aatacaataa aatccgctgc atagccttct
      121 tttatctgtc cacagacatt atcaatgcct acagacactg ctggaacata actcgccata
      181 cgcactgcct cttccggagt agcaattccc cattctacaa cattttttaa tccatctttg
      241 agcttaagga tagaaccagc taaattccct ccatctttca aacgcgcagt tccattagac
      301 acaaccacgg gcaattcccc taacatataa tctccatcag gggcacctcc agctgccata
      361 caatctgtta ttaatgccac atgattatgg cctttttgtg tcattaaaat gtcacaagca
      421 acaggatcaa cgtgatgacc atcgcaaatg agttcggcat aagtattagg taaattataa
      481 accgcaccaa ccatacctgg ttctcggtgt gtcaaaccac gcataccatt atacgcatgt
      541 acccaaactg aggcacccgc cttcactgcc tttttagctt ctttatatgt tccattagaa
      601 tgacctaaag ctaccgtaac accttgcttt gtgattgctg agacaaattc ctcaactccc
      661 tcacgttctg gtgccaaagc aatttttgta atcaagcctt ttgcagcctt ctgccattga
      721 gcaaactctt ccaagtttgg atttctcata taaatcggat tttgagctcc tttatattcc
      781 tcagtaaaat atggtccctc aaaatagatg ccttgaattt ttgctccttg ttctttgccc
      841 gctactgccg caactgattt agatgcctct tctagtcgct ttgtatctga agttagggtt
      901 gtcggcaaga aagatgtgac tccagtagat agcaagccag cactcattcg caaaattcct
      961 tctgaatcac aatccataac atcagcgcca gcaaaaccat gaatatgtgt atcaactaaa
     1021 ccaggtgcta tttggtaacc tgaataatct aaaatctcct cttgtccaga aatattttct
     1081 atccatttac caaagtgatt atcttttatt tctaaataac cgttttcctt tacatgatca
     1141 gcataaaaga atcggtcagc cttgatgtac ttcgtcatct tcttcccccc attcactctc
     1201 attataacgc tttaaggtat ttttgtaaat ggtatatacc tattttttga aaagtcatga
     1261 tttattctaa gaaagaacta tacacttgtt ataaaccact tcacttggtt gtactttcaa
     1321 ggttttaatt gctgttgctc ataattcttt tccttctcca aaactatcaa ttgctaattc
     1381 tcttatagtt tttgatgaga atggctaatt aaaaagcata gcaaaaaagt ctagtaaaac
     1441 tagactttca gggttttact gagcaacacc cgtcccatta gtagcaggtg cagtaccagt
     1501 tcctggagtc ggaacagtat tgttaacccc tggagtaact ggtgtattat tcgtttgcgg
     1561 ttgctcataa gttggtgcta cattctgatt aacgtaacta gaagtatcct gactagcagg
     1621 attagctgtc ccttgttctg tagtttcagc tactgaacta gagggagttt gattcccaat
     1681 caaaccatat tgtgtcttaa cagtatcaat acgtttttgt aaagcttctt tagattcttt
     1741 gacagttaac ttattgactg ctgcttcagc ttttaacaca ttttcttctg aaggggcttg
     1801 ttctagaatt tctactaaat ctgtagcatc tttggtagct ttcttatctg cttcgtcttt
     1861 aagatactta tcttttgctt tcttaaaacg tttttgcaaa gcttcttttt tagaagaatt
     1921 ggacaattta tttaaagcaa gttcgacatc cttcaataat ttttctgaag gtttcttctc
     1981 aaatttatct aacatatctt gtgcctcagt taattgagaa tcactgacat ggttaacact
     2041 gctttctaca gtatccattt gagtagtgcc atgatcttta aataagaaaa acgtaacaga
     2101 agcaaaaatt aataaaataa tactagcaat aagtgctaaa aattcttttt tatctatttt
     2161 catactcact cctaaagttt cttcacatta ttataacata aactaaagga cataaacaaa
     2221 cctattttag aagaatcatc gtctatcact cttattttct accggtaact tagttatatt
     2281 tgacatctca actactttgt attacaatgt agctattccg gtaacttgta ttacaatata
     2341 gacaaggata taatatgatt ccatcacaaa tgctaaaagg attacttgaa ggagctatcc
     2401 taaaaatcat acagcaagag gagacttatg cttatgaaat ctcaaaaaaa ttaaattctt
     2461 atggttttgg ggaaatttcc gaagggacaa tctaccccat tatcttaagg ctacaaagca
     2521 ataaatatat aaaaggacaa caaaagcctt cccctcaagg acctaaacgt aaatattata
     2581 cgttaaccaa tgaaggcgtg gagcaattag tccaatttaa acagaattgg gagtcactta
     2641 gtaaagcatt aaacaaacta ctctaaaaat tggagaggaa actagcatgg ataaattgaa
     2701 aaaattacaa aaagaaaata cactattaga aggaagaatt gataactcta ataaccaaac
     2761 ctatacagat atgattgtct atttgcgagg tgctagcatt agcccctacc accaggagct
     2821 cattcgaaat gatattgtca atatgttatt agaagcacaa gagcgacaag caagtttagt
     2881 tagtgtattt ggagaagata ggcacgactt tataaaccaa gttattaaat ctacacctaa
     2941 aatctctaag aaagaagaaa cattacaacg ttgggattta gccattttac tactaactat
     3001 ccaaatgatt atctttttag gaggatatct cattactgaa gcacttcaac agtcagtacc
     3061 agatctaatc cctattactc tattagatgt tctttttgca atctttatca gtattattgc
     3121 tgtcaagata gctgatacca taatatatgc tacatataac ttcgataaat cgaaagaaaa
     3181 gaaatatttc tttcgatata tgtttctaat tctttcgctt atcatagctt atatc
//
LOCUS       DATRTN010000130         3221 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.131, whole genome shotgun sequence.
ACCESSION   DATRTN010000130 DATRTN010000000
VERSION     DATRTN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.131"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..599)
                     /locus_tag="VBE22_001901"
     CDS             complement(<1..599)
                     /locus_tag="VBE22_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203328.1"
                     /translation="MVIIDVYGKITKIKLSDKLKLYISNVSDDWKESIIEDMLQEIRQ
                     QKVDMADNLKRYGKTFQTEYSISYLKEIVHANVEDYTKYNLDSIESCLQCLVDNMICL
                     FFDYEYQDMPFFDWTSNCFDGRFCEEDYAEKVMYFSNFVNHDIQNGIHMNCIYTSNMN
                     PKEHTRILSNLSFRIDSNFKGCRTTDDYITELKKMGNRI"
     gene            complement(678..1844)
                     /locus_tag="VBE22_001902"
     CDS             complement(678..1844)
                     /locus_tag="VBE22_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEO7203329.1"
                     /translation="MTQVHFTLETEDIQSLIDQSVKDNAAKQILQTVFNQLMDEQRTD
                     YIQATNYERSDDRRSQRNGYYERELTTRIGSLELKVPRTRDGEFAPTVFERYQRNEKA
                     LLASMLEMYISGVSTRKVSQIVEELCGKSVSKSFVSSLTAQLDPMVSEWQNRFLSDRS
                     FPYVMTDVIYIKIRENHRVVSKSCHIAIGISEDGEREIIGFMIQDEESDETWSLFFDY
                     LKTRGLSEVEMVISDAHKGLVKAIKKSFTNASWQRCQVHFMRNILNCIPKKDSKPFRE
                     EVKALFKFTDIETARKAKNTIVSAYSEQSKYHKACEKLDEGFEDAFQYAVVGKGQSRL
                     KSTNLLERLNEEVRRREKVIRIFPNHASANRLIGAVLIDRHEDWLSSTRKYIQF"
     gene            complement(2007..2507)
                     /locus_tag="VBE22_001903"
     CDS             complement(2007..2507)
                     /locus_tag="VBE22_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEO7203330.1"
                     /translation="MELKVYVANLGRYNEGELVGSWFTPPIDEEEMAERIGLNENYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     ENIHCYSECDSMEDVARYYVEETGQLGEVPSNLQNYIDYQALGRDMEIEGNYLITSHG
                     VFEYSN"
     gene            complement(2569..>3221)
                     /locus_tag="VBE22_001904"
     CDS             complement(2569..>3221)
                     /locus_tag="VBE22_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEO7203331.1"
                     /translation="EVTLDETNEFTYYKGYKRDWNTLCTYLKIRAPFDDLDFFESHKD
                     LLTQTASSIYDKQGDNVLVDTILVPLPENYEVINFSQLKISDVISQAIEDAESFMSNG
                     EYQRAFDRVHTAFHGYLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIV
                     KTTIRSSNGMISSLNEARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
BASE COUNT          959 a          611 c          505 g         1146 t
ORIGIN      
        1 tcaattcggt tgcccatttt tttaagttcc gtaatgtagt cgtctgtagt cctgcaccct
       61 ttaaagtttg aatcaattct aaaacttaga tttgataata tcctagtatg ctctttagga
      121 ttcatattac ttgtataaat gcagttcata tgaataccat tttgtatatc atgatttaca
      181 aagttactaa aatacatcac tttttcagcg taatcctctt cacaaaaacg accatcaaag
      241 caattgcttg tccaatcaaa aaaaggcata tcctgatatt catagtcaaa gaatagacat
      301 atcatattat caacaaggca ttgaagacat gactcaatag aatctaaatt atattttgta
      361 tagtcttcaa cattggcgtg aacgatttct ttcaaataag atatagaata ttctgtttga
      421 aatgttttac catacctttt caaattatct gccatgtcaa ctttttgttg tcgaatttct
      481 tgtaacatgt cttctataat gctttccttc cagtcatcag aaacattaga tatatatagt
      541 ttcaatttat ctgacaattt tatttttgtt attttcccat aaacatctat tattaccaat
      601 tgaacaatcc tccttaaagt caagtccata aaattgtgta aaatacagta cactgtttta
      661 aagctttaac ctataggtta aaattgaata tattttctgg tagagcttag ccagtcttcg
      721 tgacggtcaa taagaactgc accgattaaa cgattggcag aggcgtgatt cggaaagatt
      781 cggataactt tttctctgcg tctgacttct tcatttaacc gttcaagaag attcgtgctt
      841 tttaatctcg attgaccttt accgacaacg gcgtattgaa aggcatcttc gaatccttca
      901 tctagttttt cacaggcttt atggtatttt gattgttcgc tataggcact tactatagta
      961 tttttagcct tgcgagccgt ttcaatatcc gtaaatttga agagtgcttt gacttcttct
     1021 ctgaaaggtt ttgaatcttt cttagggata caattgagga tattacgcat aaagtgaacc
     1081 tggcatcgtt gccatgaagc gtttgtaaat gacttcttga tggctttgac taagcctttg
     1141 tgggcatcgg aaatgaccat ttccacttca cttaaccctc tggttttgag gtagtcgaag
     1201 aagagagacc aagtttcgtc actttcttca tcttgaatca taaagccaat aatctcacgc
     1261 tctccatctt ctgatatgcc aatcgctata tggcagcttt tagatacgac acgatgattc
     1321 tctctgattt tgatgtagat aacatctgtc ataacataag ggaaagacct gtctgataga
     1381 aaacgatttt gccattcaga gaccataggg tctaactggg cagttaaact ggagacaaag
     1441 gatttagaga ctgatttacc gcatagttct tcaacgattt gagagacttt acgtgtagag
     1501 acaccagaaa tatacatctc taacatagag gctaacaaag ccttctcatt gcgttgatac
     1561 cgctcaaata cagtcggtgc aaattcaccg tcgcgtgttc ggggaacttt caactcaaga
     1621 gagccaatac gagtcgttaa ttctctttca taatagccgt tgcgttggct tcttcgatcg
     1681 tctgatcgtt catagtttgt ggcttgaata taatctgttc gctgctcgtc catcaactga
     1741 ttaaatacag tttgtaaaat ttgtttagcc gcattatctt tgaccgactg gtcaattaaa
     1801 ctttgaatat cttctgtttc cagtgtaaaa tgtacttggg tcatatgatt gcctcctggg
     1861 tatgttttta gtggttaaaa acattgtacc gtaaagaggc gttcatatgg cctttttgct
     1921 tttacacaat tatatggact ttatcctcct taattcagaa cgtataaaat tattatagca
     1981 gtattgctag tttagagtaa tccatgttaa ttagaatact caaatactcc atgagaggtg
     2041 ataagataat ttccctctat ttccatatca cgtcccaaag cttgataatc aatataattt
     2101 tgtaaattag aaggtacttc accaagttgt cctgtttctt ccacataata acgggcgaca
     2161 tcttccatag aatcacattc actataacaa tgaatattct ctttgttttc cagtaattct
     2221 tccaagctac taaaccacat gccttgaatt tctttgagtt cattataaat aggtgtacct
     2281 tcgatttctt gaatggcttc acatagacgg ttgatttcac taattggcgt gtattcgtcc
     2341 acatcaaaag gaagctcaaa atcatgtatg gcgtattctt cgtagttctc atttaaaccg
     2401 attctttctg ccatttcttc ctcatcaatt ggtggagtaa accatgatcc aactaattca
     2461 ccttcattat accgtcccaa atttgccaca tagactttca attccaattc ttatcacatc
     2521 ctttttataa aatatagtca aaaaaaaagt agcaatcagt taatgacgct acttatcaag
     2581 gtatccgcta atataatctg taactgtact tgaaatacct attattagtt ttgcttctct
     2641 ctttccaata atatttgtat taggatgtgc aagtgaatgc ctatttcttg cttcattaag
     2701 agagctaatc attccatttg atgaccttat cgttgtcttc actatatcag caagttcagt
     2761 aggttgaatt tctttttcaa ttaattgttg tatcctacta tataatttag atagattttc
     2821 gtctctagga actgtaattt catatttttt tagtatttca attaaatatc catgaaaagc
     2881 agtgtgaact ctgtcaaagg cacgttgata ttcaccatta ctcatgaaag actcagcgtc
     2941 ttcaatagct tgtgaaatca catcactaat tttcaactga ctaaagttaa ttacttcgta
     3001 attctctgga agtggtacta aaatagtatc cactaataca ttatcacctt gtttatcata
     3061 tatggagctt gctgtctggg taagcaaatc tttatgactt tcaaaaaagt ctaaatcatc
     3121 aaaaggagct cttattttaa gataggtgca aagcgtattc caatcccttt tgtatccttt
     3181 ataataagta aactcgttag tctcatctaa agttacctct g
//
LOCUS       DATRTN010000131         3181 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.132, whole genome shotgun sequence.
ACCESSION   DATRTN010000131 DATRTN010000000
VERSION     DATRTN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.132"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..604)
                     /locus_tag="VBE22_001905"
     CDS             complement(<1..604)
                     /locus_tag="VBE22_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEO7203332.1"
                     /translation="MKKCFLAICLALSFFMVSVQADEVDYNIPHYEGNLTIHNDNSAD
                     FTEKVTYQFDSSYNGQYVTLGTAGKLPDNFDINNKPQVEVSINGKVRKVSYQIEDLED
                     GYRLKVFNGGEAGDTVKVNVQWKLKNVLFMHKDVGELNWIPISDWDKTLEKVDFWIST
                     DKKVALSRLWGHLGYLKTPPKIRQNNNRYHLTAFNVNKRLE"
     gene            740..2290
                     /locus_tag="VBE22_001906"
     CDS             740..2290
                     /locus_tag="VBE22_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO7203333.1"
                     /translation="MKKILLSLFTALLITFGGMTSIQADEYLRVGMEAAYAPFNWTQN
                     DNTNGAVPIEGTDQYANGYDVQVAKKLAKKLNKKVVVVKTKWEGLVPALTSGKLDMII
                     AGMSPTEERKKEINFSKPYYISEPTLVVNAEGKYTNAKNISDFKNAKVTAQQGVYLYN
                     LIDQINGVKKEVAMGDFNQLRQAVESGVVDAYVSERPDATSAQTANPKLKMIELHQGF
                     KTSDADTNISVGMRKGDNRINQVNQVLESISRDKQIALMDKMIKEQPSVKKEKNGKPN
                     FFEQMATILKNNGSQFLRGTATTLLISMVGTIVGLFIGLLIGVFRTAPKSDNKLKAAL
                     QKLLGWLLNIYIEVFRGTPMIVQSMVIYYGTAQAFGVSLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFSVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFILTFTVTRILRYIEKRFDSDNYT
                     TGANQLQV"
     gene            2300..3040
                     /locus_tag="VBE22_001907"
     CDS             2300..3040
                     /locus_tag="VBE22_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7203334.1"
                     /translation="MTQAILEIKHLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STFLRSINLLEEPSGGEILYHGHNVLEKGYDLNNYREKLGMVFQSFNLFENLNILENA
                     IVAQTTVLKRERQEAEKIAKENLNAVGMTEQYWKAKPKQLSGGQKQRVAIARALSVNP
                     EAILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFAKEVSDRVIFMDKGI
                     IAEQGTPKQLFENPTQERTKEFLQRFLK"
BASE COUNT         1085 a          603 c          533 g          960 t
ORIGIN      
        1 attctaatcg tttgtttaca ttaaaagctg tcaaatggta acgattatta ttttgtctta
       61 ttttaggagg agttttaaga taacccaagt gcccccaaag acgagaaaga gcaacctttt
      121 tgtcagttga tatccaaaaa tctactttct ctaacgtttt atcccagtcg ctaataggaa
      181 tccagttaag ttcaccaaca tccttatgca taaatagaac attttttagt ttccactgaa
      241 cattgacttt aacagtatca cctgcttcac caccattaaa cactttcaaa cggtagccat
      301 cctccaaatc ttctatctgg taactaactt tccttacttt accattaatt gaaacttcaa
      361 cctgtggctt attattaata tcaaaattgt caggtaactt acccgccgta cctaatgtga
      421 catactgtcc attataggac gaatcaaatt ggtaagtaac cttctctgta aaatcagcac
      481 tattatcatt gtgaatagtt agattaccct cataatgagg aatgttatag tccacctcat
      541 ctgcttgaac tgaaaccata aaaaaactaa gagctaaaca aatagccaaa aaacatttct
      601 tcattatcat cctccctcct ttgtttgtat accattttaa cataaaaata caattaatcg
      661 ctttcaataa gataatgata ttgaaatcga ccttgttttt cggtaaaata ggaggaagta
      721 acttatcgga ggctgttgaa tgaaaaaaat attactaagt ttattcactg ctttgcttat
      781 cacctttgga ggtatgacaa gtatacaagc agatgaatat ctacgtgtcg gtatggaggc
      841 agcctatgct cctttcaact ggacacaaaa tgataacaca aatggtgccg ttcctattga
      901 aggaactgat caatatgcta atggttatga tgtacaagtc gctaaaaagt tagctaaaaa
      961 gttaaataaa aaagttgttg ttgtcaaaac aaaatgggaa ggccttgttc cagcactaac
     1021 ttctggaaaa ctcgatatga ttattgcagg aatgagtcct accgaagaac gtaaaaaaga
     1081 aattaatttt tcaaagcctt actacatttc agaacctact ctagttgtta acgctgaagg
     1141 aaaatatact aatgctaaaa atattagtga tttcaaaaat gctaaagtaa ctgcccaaca
     1201 aggagtctac ttatacaacc tcattgatca aatcaatggt gtcaaaaaag aagttgccat
     1261 gggggacttt aatcaattaa gacaagctgt agaatctggt gttgtagacg cctatgtttc
     1321 agaacgtcca gatgcaactt ccgctcaaac tgctaatcct aaattaaaaa tgattgaact
     1381 gcaccaaggg tttaaaacat ctgatgctga tacaaatatt tctgttggga tgcgtaaagg
     1441 tgacaatcgt attaatcaag tcaatcaagt tctcgaaagc atttctagag ataaacaaat
     1501 cgccttgatg gataagatga ttaaagaaca accatccgtt aaaaaagaaa aaaatggaaa
     1561 accaaacttc tttgaacaga tggcaactat tttaaaaaac aatggttctc aattcctcag
     1621 aggaacagct acaaccctcc taatttctat ggtaggaact atcgtcggat tatttatcgg
     1681 ccttctcatc ggtgttttcc gtacagcacc aaaatctgac aataagttaa aggctgcctt
     1741 acaaaaatta ttaggttggt tacttaacat ctatattgag gtcttccgcg ggacacctat
     1801 gattgtacaa tcgatggtta tctactacgg aacagcacaa gcttttggag tttcgctgga
     1861 ccgtacacta gcagctatat ttattgtttc aatcaatact ggtgcttata tgagtgaaat
     1921 tgtgcgtggt ggtattttct ctgtcgataa aggacaattt gaagccgcaa ctgcacttgg
     1981 tttcacccac ggacaaacca tgcgaaaaat tgttctacca caagttgtac gtaacatact
     2041 cccagctact ggtaatgagt ttgttatcaa tatcaaagat acctctgtac tgaacgttat
     2101 ctctgttgtc gaactctact tctctggtaa tactgttgca acacaaacct accaatattt
     2161 ccaaacattc accattattg cgattattta cttcatctta accttcaccg taacacgtat
     2221 cttacgttac attgaaaaac gttttgattc agataattac acaaccggtg cgaatcaatt
     2281 acaagtctag gaggttggta tgacacaagc tattttagaa attaaacatt tgaaaaaatc
     2341 ttacggaagc aatgaagtgt taaaagatat ttcgctttcg gtcaacaaag gcgaagttat
     2401 ttctatcatt ggttcttctg gatcaggaaa atcaactttc ctacgttcta ttaacctctt
     2461 agaagaacca agtggaggag aaatcttata tcacggtcac aatgttcttg aaaaaggtta
     2521 tgatttaaat aattatcgtg aaaaacttgg gatggtcttt caatctttta acctctttga
     2581 aaatttaaac atcttagaga atgctattgt cgcacagact actgttctaa aacgtgaacg
     2641 ccaagaagct gaaaagattg ctaaagaaaa cttaaatgct gttggcatga ctgagcaata
     2701 ttggaaagct aaaccaaaac aattatcagg tgggcaaaag caacgtgtcg caatcgcccg
     2761 agcactttct gttaacccag aagctatact ttttgatgaa ccaacttcag ccttggatcc
     2821 agaaatggtt ggtgaggtcc ttaaaacaat gcaagatcta gctaaatctg gattaacaat
     2881 gataattgtg acacacgaga tggaatttgc taaagaagta tcagaccgcg ttattttcat
     2941 ggacaaagga atcattgcag agcagggtac tcctaaacaa cttttcgaaa atccaacaca
     3001 agaaagaact aaggaatttc tacaacgctt cttaaaataa aatttattca catttaaata
     3061 tagactattt atacccctcc agttccattg tcatcattgc aatggaactg gaggggattt
     3121 tttattgcta catctttggt gaaatccacg actagctcaa ctatagatca aattagacag
     3181 t
//
LOCUS       DATRTN010000132         3163 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.133, whole genome shotgun sequence.
ACCESSION   DATRTN010000132 DATRTN010000000
VERSION     DATRTN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.133"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            124..696
                     /locus_tag="VBE22_001908"
     CDS             124..696
                     /locus_tag="VBE22_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO7203335.1"
                     /translation="MRKRSILPILAVLFVFMSIFSVYSIFRGDKAKEKASVIKQASQT
                     SQTSKKEVLQKKTYPNLNKYSNLEIHVSSTRQTMTITSNDKVIFKTIVSTGAKESPTP
                     KGTFVIEPERGDFFYNASSKEGAYYWVSFKEHGIYLFHSVPTDQQGNEIPEEAKQLGK
                     AASHGCVRMSRADAKWFYENIPQGTTVTIK"
     gene            972..1823
                     /gene="ylqF"
                     /locus_tag="VBE22_001909"
     CDS             972..1823
                     /gene="ylqF"
                     /locus_tag="VBE22_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEO7203336.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADPIRTKEWRDFYESQGLKTLAINSKEQSTVKKVTDIAK
                     ILMSDKIAKLRGRGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTYY
                     PDRLKERFKSINLEDEAPEIIMALTQKLGYRDDYDRFYNLFVKEVRDGKLGRYTLDIV
                     GEHDGNN"
     gene            1810..2571
                     /locus_tag="VBE22_001910"
     CDS             1810..2571
                     /locus_tag="VBE22_001910"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEO7203337.1"
                     /translation="MATIKEIKAILETIVDLKDKRWQEYQTDSRAGVQKAILQRKKNI
                     QSDLDEEARLEQMLVYEKKLYIEHINLIAGIDEVGRGPLAGPVVAAAVILPPNCKIKH
                     LNDSKKIPKKKHQEIYQNILDQALAVGIGIQDSQCIDDINIYEATKHAMIDAVSHLSV
                     APEHLLIDAMVLDLSIPQTKIIKGDANSLSIAAASIVAKVTRDKIMSDYDSTYPGYAF
                     SKNAGYGTKEHLEGLQKYGITPIHRKSFEPIKSML"
     gene            2592..3140
                     /locus_tag="VBE22_001911"
     CDS             2592..3140
                     /locus_tag="VBE22_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEO7203338.1"
                     /translation="MTEKEKMLAGQYYRPSAPELRKDREVALKNMQAFNNEDNSSKRN
                     VILQKWFGATGKSIHMEQRFVCDYGCNIYVGENFYANFNQTFLDVCEIRIGDNCMFGP
                     NCQLLTPLHPLDPIERNSGLEYGAPIQIGNNVWLGGGVTILPGVVLGDNVVVGAGSVV
                     TKSFENNVVIAGNPAKIIKKLA"
BASE COUNT         1163 a          523 c          571 g          906 t
ORIGIN      
        1 tctactaaaa attccagtaa atatttctgt ttgattacaa atgttacaaa aatatttgac
       61 aaccttcttt cagaaactta taatagattt gtccataaca gagcaaaaga gagaggatag
      121 tacatgagaa aacgttcaat tttgccaata ttagctgtct tatttgtttt catgagtatc
      181 tttagcgtat attctatttt tcgtggtgat aaagccaaag aaaaagcaag tgtcataaaa
      241 caagcatctc aaacaagtca aacttcaaaa aaagaggtct tacaaaagaa gacttatcca
      301 aatctaaata aatattcaaa tctagaaatt catgtttcat ctacgaggca aacaatgaca
      361 ataacctcga atgacaaggt catctttaaa acgattgttt caacaggagc aaaagagagc
      421 cctaccccaa aaggaacttt tgtcatagaa ccggaacgag gagatttttt ctataacgcc
      481 tcatcaaaag aaggagccta ttattgggtc tcctttaaag aacatggtat ttatctcttt
      541 catagcgttc cgactgatca acaaggaaat gagattccag aagaagcaaa gcaattagga
      601 aaagccgcct cacatggctg tgtgcgtatg tcaagggcgg atgcaaaatg gttctatgaa
      661 aatattcctc aaggaacgac tgttaccatc aaatagtctt aatgtggtaa aaatcatgtt
      721 attaaaagct cggagtaaac ttccagagct ttttcgtata tcccttaaac tcaattagta
      781 aacgaaacct atcagcggaa taaatgtata aagtatttcg atatcattaa aaaaatgata
      841 gaataatagt gttaatcgaa tattttatcg acctttatat gcggttgaaa aagaataaaa
      901 ctaaaaaatt agacctctaa caaatagatg tagactttta aggtagtcta tacataagga
      961 gaatttattt catggctact attcaatggt tcccaggaca catgtccaaa gctcgccgtc
     1021 aagttcaaga aaatattaag catgttgatt ttgttacaat tttagtggat gcaaggttac
     1081 ctctttctag tcaaaatcca atgctaacca aaatcgtcgg agacaaaccc aaactaatga
     1141 tcctcaataa agctgatcta gcagatccta ttcgaactaa agaatggaga gatttttatg
     1201 aatctcaagg tttaaagaca ttagctatta attctaaaga acaatcaaca gttaaaaaag
     1261 taaccgacat cgctaaaata ttaatgtccg ataaaattgc taagctacgg ggacgtggta
     1321 ttcaaaaaga gacacttcgt acgatgatta ttggaattcc gaatgcaggt aaatcgactc
     1381 taatgaatcg cctagcaggt aaaaaaattg cagtggttgg taataaacca ggtgtcacaa
     1441 aaggtcagca atggctcaaa tctaataagg aactggaaat tttggacact cctggtatct
     1501 tgtggcccaa atttgaagat gaattagttg gattaaagct agcattaaca ggggcaatca
     1561 aagatcaatt attgcctatg gatgaggtaa caatatttgg attgaattac tttaaaacct
     1621 attatcctga tcgtctcaaa gagcgtttta aaagcattaa tttagaggat gaagctcctg
     1681 aaatcatcat ggccttaaca caaaaattag gttatcgtga cgattacgat cgcttttata
     1741 atttatttgt caaagaagtt cgcgatggta aattaggacg ctatacgtta gatatcgtcg
     1801 gagaacatga tggcaacaat taaagaaatc aaagcaattt tagagactat tgttgattta
     1861 aaagacaaac gttggcaaga atatcaaaca gattcacgcg caggtgttca aaaagcaatc
     1921 cttcaacgaa aaaaaaatat tcagtcagat ttagacgaag aagcgcgctt agaacaaatg
     1981 ttggtctacg aaaaaaaact ttacattgaa catataaacc ttattgcagg aatcgatgaa
     2041 gtaggtagag gtcctttagc gggaccagta gtagctgctg ctgttattct tccaccaaac
     2101 tgtaaaataa aacatctcaa tgactccaaa aaaattccaa agaaaaagca tcaagaaatc
     2161 tatcaaaata ttttagatca agcgttagca gttggtattg gtattcaaga cagccaatgt
     2221 attgatgata tcaacattta tgaagctaca aaacatgcta tgattgatgc agtttctcat
     2281 ttatctgtgg ctcctgaaca tcttttaatt gatgctatgg ttttagatct atcaattcca
     2341 caaactaaaa ttatcaaagg tgatgctaat tcactctcta ttgcagcagc ttcaatcgta
     2401 gctaaagtga ccagagataa aataatgtct gattatgata gcacatatcc tggatatgca
     2461 ttttctaaaa atgctggcta tggtactaaa gagcatttag aaggtttgca aaaatacggt
     2521 attactccga tacatcgtaa aagttttgaa cctataaaat caatgttgta aataatacgg
     2581 aagaggaaaa gatgaccgaa aaagaaaaaa tgttagcagg acaatattat cgtccatcag
     2641 cacctgaact acgcaaggat agagaagttg cactaaaaaa tatgcaagcc tttaacaatg
     2701 aagataatag tagcaaacgt aacgtcattt tacaaaaatg gtttggtgct actgggaaga
     2761 gtatccatat ggaacaacgt tttgtctgcg attatggttg taatatttat gttggtgaaa
     2821 acttctacgc caattttaac caaactttct tagatgtttg tgaaattcgt attggcgata
     2881 actgtatgtt tggcccaaac tgtcaactcc tcaccccact ccatcctcta gatccaatag
     2941 aaagaaattc aggattagaa tacggtgccc caatccaaat cgggaataat gtctggctag
     3001 gtggaggagt gactatttta ccaggtgttg tattaggaga taatgtcgtc gttggcgctg
     3061 gaagtgtcgt tacgaaatca tttgaaaata acgttgttat agcaggaaac cctgctaaga
     3121 taatcaaaaa attagcttaa aattagaaaa aacctttaat tat
//
LOCUS       DATRTN010000133         3109 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.134, whole genome shotgun sequence.
ACCESSION   DATRTN010000133 DATRTN010000000
VERSION     DATRTN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.134"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..1651
                     /locus_tag="VBE22_001912"
     CDS             <1..1651
                     /locus_tag="VBE22_001912"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEO7203339.1"
                     /translation="AQSVALKPIAEIVEQVGIGFDDIELYGKYKAKLSFDKIEAVKSQ
                     KVGKLILVTAINPTPAGEGKSTMSIGLADALNKIGKKTMIALREPSLGPVMGIKGGAA
                     GGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELDIDQRRVIWKRVV
                     DLNDRALRQVIVGLGSPVNGIPREDGFDITVASEIMAILCLATDLSDLKKRLSNIVVA
                     YSRDRKPIYVKDLKIEGALTLILKDAIKPNLVQTIYGTPALVHGGPFANIAHGCNSVL
                     ATSTALRLADYVVTEAGFGADLGAEKFLDIKTPNLPTSPDAIVIVATLRALKMHGGVS
                     KEDLSQENVEAVKRGFTNLERHVNNMRQYGVPVVVAINQFTADTESEIATLKTLCSNI
                     DVAVELASVWEDGADGGLELAQTVANVIETQSSNYKRLYSDEDTIEEKIKKIVTKIYG
                     GNKVHFGPKAQIQLKEFSDNGWDKMPICMAKTQYSFSDNPNLLGAPTDFDITVREFVP
                     KTGAGFIVALTGDVLTMPGLPKKPAALNMDVLEDGTVVGLF"
     gene            1770..>3109
                     /locus_tag="VBE22_001913"
     CDS             1770..>3109
                     /locus_tag="VBE22_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEO7203340.1"
                     /translation="MIWRGEKLISNKVKIVRLLNKSKKSLLRGIFSRTTVIAILLILQ
                     LLFLLASYSWLEQYRVWLATVEHILTIGAVLYLVNSEMDALSRVTWLILVMIAPLLGA
                     MFLMYTKFDWGYRGLKQRLETLIDESQIYLEDDPETLNQLKSSTSTTYHLVQYFEKAH
                     GNFPVYRNTDVTFLPTGETFFEKMKEELLKAKKYIFLEFFIIDEGIMWGEILSILEQK
                     VEEGVEVRILYDGMIEITKLSFDYTKRLEKIGIKAKAFSPISPFISTYYNYRDHRKIV
                     VIDGVVGMTGGVNLADEYINHIELFGHWKDSGIMLKGKAVDSFLLLFLQMWSITEEKM
                     LVAPYLGVHDDLVENEGYVIPYGDSPLDTDKVGENVYIDILNHAREYVYIMTPYLILD
                     SELEHAIQFAAERGVDVRIIMPGIPDKPIPYALAKTYYQALTKSGVKIYEYTPG"
BASE COUNT         1000 a          472 c          650 g          987 t
ORIGIN      
        1 agcgcaaagc gtcgccctaa agcctattgc tgagattgtt gagcaagtag gaattggttt
       61 tgatgatata gaattgtatg gtaaatacaa agcaaaatta tcttttgata agattgaagc
      121 tgtaaaatct caaaaagtag gaaagttaat tttagtaact gctattaatc cgacaccagc
      181 aggtgaggga aaatccacta tgagcattgg attagctgat gctttaaata aaattggtaa
      241 aaaaacgatg attgctcttc gcgaaccttc tttaggtcct gtaatgggta ttaaaggagg
      301 agctgctgga ggtggatacg cacaagtact accaatggaa gatattaatt tgcattttac
      361 tggtgatatg cacgcaatca caactgctaa taatgcttta tcagctctac ttgataatca
      421 tattcatcaa ggcaatgaat tagatattga ccaacgtcga gtgatttgga agcgtgtagt
      481 tgatcttaat gatcgtgctc ttcgtcaggt gatcgttggc ttaggaagcc ctgtcaatgg
      541 gataccacgt gaagatggct ttgatattac tgttgcttct gaaataatgg ctattctatg
      601 tttagctact gacctatcag atttaaagaa acgcctgtca aatattgttg tggcttactc
      661 aagggaccgt aaaccaatct atgttaaaga tttgaagatt gaaggagctc tcacacttat
      721 cctaaaagat gctatcaaac ctaatttggt tcaaaccatt tatgggacac cggctcttgt
      781 ccatggggga ccatttgcta atattgcaca tggatgtaat tctgtcttag cgacttcaac
      841 agctctacgc ttagcagatt acgttgttac tgaagctggt tttggagcag acttgggcgc
      901 tgaaaagttt cttgatataa aaacgccaaa ccttccgact tctcccgatg cgattgttat
      961 tgttgctaca ttgcgtgcat tgaaaatgca tggaggtgtt tcaaaagaag atctctcaca
     1021 agagaacgtc gaagcagtta aaagaggttt tacaaatctc gagcgtcacg ttaacaatat
     1081 gcgtcaatat ggtgttccgg tagttgtagc tatcaatcag tttactgcag atacagaaag
     1141 tgaaattgca actcttaaaa ccttatgtag taatattgat gtggcagttg aattagcaag
     1201 tgtgtgggaa gatggagcag acggtggcct tgaacttgca cagactgttg ctaatgtgat
     1261 tgaaacacaa tcatccaatt acaaacgctt atatagtgat gaagacacta ttgaagaaaa
     1321 aattaaaaaa attgttacta aaatatatgg tggtaataaa gttcattttg gacctaaggc
     1381 acaaatacaa ttaaaagagt ttagtgacaa tggctgggac aagatgccta tttgtatggc
     1441 aaagacacaa tatagctttt ctgataatcc aaatttactt ggtgctccaa ctgactttga
     1501 tataactgtt cgtgaatttg ttccaaaaac aggagctggt tttatagttg ctcttactgg
     1561 agacgtattg acaatgcctg gtttacctaa aaaacctgca gctctcaata tggatgtttt
     1621 agaagacggt acagtcgttg gattatttta aataataaag aggtagggct tgtgatgcta
     1681 gcctcttttt attggggatt agattttaac tagagataat ttagaataat agaatgctat
     1741 tcaagtgaca gtatgctata ataaaatgat tgatttggag gggggaaaaa cttatcagta
     1801 ataaagtcaa aattgttcgt ttacttaata agagtaagaa aagtttacta aggggtatat
     1861 ttagtcgaac aaccgttatc gctattttat tgattttaca attattgttt ttactagcct
     1921 cttactcatg gctagagcag tatcgtgttt ggttagcaac cgttgaacat atcttaacaa
     1981 ttggagcggt cttatattta gttaatagtg aaatggatgc tttgtctcga gtaacttggt
     2041 taatattggt aatgattgcc cctttactag gggcgatgtt cctaatgtac accaaatttg
     2101 actggggtta tcgtggtttg aaacaaagat tggagacatt gattgatgag agtcagattt
     2161 atctagagga tgatcctgaa actttgaatc agcttaagtc gagtacatcc acaacatatc
     2221 acttggttca atattttgaa aaagcacatg gaaatttccc agtttatcga aatactgatg
     2281 tgacatttct accaacggga gaaacattct ttgaaaaaat gaaagaagaa ctactaaaag
     2341 ctaagaagta tatcttttta gaattcttta tcattgacga aggaataatg tggggagaaa
     2401 tacttagtat tttagaacaa aaggtggaag aaggtgttga agtccgtatc ttatatgatg
     2461 gtatgataga aattacaaaa ttatcctttg attatacgaa acgacttgag aaaataggaa
     2521 taaaagcaaa agcattttct ccgatttctc cttttatttc aacttattat aactatcgtg
     2581 accatcgaaa aattgttgtt attgatggag tagtaggcat gactggtggt gtaaacttag
     2641 ccgatgagta tattaatcac attgagttat ttgggcattg gaaagattca ggtattatgt
     2701 taaaggggaa agcagtggat agtttcctcc tacttttctt acagatgtgg tccattactg
     2761 aggaaaaaat gttagtagcg ccttacctcg gtgtacacga tgatttagtt gaaaatgaag
     2821 gctatgttat tccttatgga gattctcctt tagacactga taaagtaggg gaaaatgttt
     2881 atatagatat tcttaatcat gctagagaat atgtttacat catgacacct tatttaatat
     2941 tagacagtga attggaacat gccattcagt ttgctgcgga acgtggtgta gatgtacgta
     3001 ttattatgcc gggtatccca gataagccaa tcccttatgc tcttgctaaa acttactatc
     3061 aagcattaac aaaatcaggt gttaaaatat atgaatacac ccctggatt
//
LOCUS       DATRTN010000134         2975 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.135, whole genome shotgun sequence.
ACCESSION   DATRTN010000134 DATRTN010000000
VERSION     DATRTN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.135"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..142
                     /locus_tag="VBE22_001914"
     CDS             <1..142
                     /locus_tag="VBE22_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEO7203341.1"
                     /translation="KDAIKGKTLNSTSISELLIPISNYREMKKIVSKVDLLFQKVAQL
                     SD"
     gene            complement(96..1406)
                     /locus_tag="VBE22_001915"
     CDS             complement(96..1406)
                     /locus_tag="VBE22_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO7203342.1"
                     /translation="MPYDIPASWEWVRLRNISSLSFFPNISGDKIPNYSWVLDMEDIE
                     KETGRLVRKNYKTEKSSYKSNKVYFSKDTVLYAKLRPNLKKVIISDEDGFATTELIPI
                     KIFGGISAEYMRYCMISPSYYFNIIKSVYGVKMPRVNATFLNSTLLPLPPLSEQKRIV
                     EQIERALEKVDAYSESYNKLQELDKSFPDKLKKSILQYAMQGKLVPQDPNDEPVEVLL
                     EKIQAEKQKLYEEGKLKKKDLAEIVVTKGDDNSPYGKIPKNWSFLTIKDIFSITTGLS
                     YKKTDLAITKNGVRIIRGGNINPLSFKILDNDYYIDPKFITSETVYLKRNQLLTPVST
                     SLEHIGKFARIDKDYPNTAAGGFVFQLTPFVSSDVLSKYLLFSLSSPIFYEQLKSITK
                     LSGQALYNIPKTKLNELLVPLAPETEQKRISQRVEQLFEKVNQL"
     gene            1527..2960
                     /locus_tag="VBE22_001916"
     CDS             1527..2960
                     /locus_tag="VBE22_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HEO7203343.1"
                     /translation="MNQTLSHYQFSPETQYLDRKSARKKPSELLKHLIAFANADGGQL
                     VIGIEDDKQNNIITGFKDGRAYPIDDFKKIDREMRDTPLDLSFEEIPVRNHKGEDDVI
                     LVISVELSSNRVIAAPNDEVYLRQGDETVKLSYEQRTQLSYDKGQRFFEDEVVADATL
                     EDIDDDLVQDFKNRFDIADRSTEEILKARRFLVNGKLTKAAILLFGKYPSAFFPQARV
                     RFQRFDGTDMGTGSSFNVIKEVTFDDALPTLIIKARDFIRTQLREFQYLDDNGQFQIL
                     PEYPEFAWFEGVVNAVTHRDYSVYGDHIRVLMFDDRLEIHSPGKLPNIVTVDNIKHER
                     FSRNPRIARTLTEFGWVREMNEGVKRIYSEMESAFLHEPKYSEPGNKVILTLENNIVS
                     RHLRTQDSLEKQFTDFGDLNADEQLLIHYMYNSGEKMTTAKAIELTGRSRKFIVKTMK
                     KLQELGMVDWYGANKNDRNQYYTLTKN"
BASE COUNT          919 a          538 c          584 g          934 t
ORIGIN      
        1 aaaagatgct ataaaaggca aaacactaaa tagcaccagt atttcagagt tattgatacc
       61 tatttcaaat tatcgggaaa tgaaaaaaat tgtatctaaa gttgatttac tttttcaaaa
      121 agttgctcaa ctctctgact aatgcgtttt tgctctgttt caggagctag gggaacaagg
      181 agttcgttta attttgtttt aggaatatta tataaagcct gtccagatag ctttgtaatt
      241 gatttcaatt gttcataaaa gattggtgat gacaggctaa ataatagata ttttgacaat
      301 acatctgatg aaacaaaagg tgttaattga aatacaaatc caccagcagc ggtattggga
      361 taatctttat caattctagc aaattttcca atatgttcta aagatgttga aacaggtgtt
      421 aatagctgat ttcgtttcaa atagactgtt tctgaagtga taaattttgg atcaatgtag
      481 taatcattat ctaatatttt aaaggataat gggttgatat ttcctccacg aataattctg
      541 acaccatttt ttgtgatggc tagatcagtt ttcttatacg ataatcctgt ggttattgaa
      601 aaaatatctt ttatggtaag aaaagaccaa tttttgggta tcttcccata aggagagtta
      661 tcatctcctt ttgtcaccac tatttctgcc aaatctttct tcttgagttt accttcctca
      721 tagagttttt gcttttcagc ttggattttt tcaagcaaaa cctcaacggg ttcatcgtta
      781 gggtcttgag gaaccaactt accttgcatc gcatattgaa gaatagattt tttcaactta
      841 tctggaaagg atttgtccaa ttcttgaagt ttattgtaac tttcggaata tgcatctacc
      901 ttttccaagg cacgttcgat ttgctccaca atccgtttct gttcagagag aggcgggagg
      961 gggaggagag ttgaatttag aaacgtagca tttactcttg gcattttaac accataaacc
     1021 gatttgatga tgttgaaata ataagatgga gaaatcatac agtatctcat atactctgct
     1081 gaaatacccc caaaaatttt aatcggaata agttcggttg ttgcaaaacc atcttcgtca
     1141 gaaatgataa ctttctttag gttggggcgt agcttagcat ataaaacagt atccttagaa
     1201 aaatatactt tattgctttt ataagatgat ttttctgttt tatagttttt tcgaactaat
     1261 cttccagttt ctttttctat atcttccata tctaaaaccc aactataatt cggaatttta
     1321 tctccagata tattagggaa aaaactcaac gaagatatat ttcgcaacct cacccattcc
     1381 caactagctg gaatatcata gggcacttcc acttccttaa tcgtcccgtc tgataacttc
     1441 tcataaggtt tcttagcaca atgaattgga agcaagaaag aatgggagat gtgttataat
     1501 agagctataa cactcaggag aatgacatga atcaaaccct atcccactac cagttctcac
     1561 cagagactca atacttggac aggaaatctg cacgtaaaaa gccgtctgaa cttcttaaac
     1621 acctgattgc ctttgctaat gcagatggtg gccagctggt aatcggtatt gaggacgaca
     1681 agcaaaataa catcattact ggcttcaaag atggcagagc ctatcccatt gatgacttca
     1741 aaaagattga ccgtgagatg cgtgatactc ctttggactt atcttttgaa gaaatccctg
     1801 tgcgaaacca taagggtgaa gacgatgtga ttttggttat ctcagttgaa ttatcttcaa
     1861 atcgagtgat tgctgcgcct aatgatgaag tttacctgcg tcaaggggat gaaacggtaa
     1921 aattaagtta tgaacaacga actcagctca gctatgataa aggacaacgt ttctttgagg
     1981 atgaagttgt ggctgatgcg accttggaag acatcgatga tgacctggtt caggacttca
     2041 aaaatcgctt tgatattgca gaccgttcca ccgaggaaat tctcaaagct cggcgttttc
     2101 tggtcaatgg aaaattgacc aaggctgcta tcttgctttt tggcaaatac ccttcagctt
     2161 tcttccctca agctcgtgtt cgcttccaac ggtttgatgg cacggacatg gggacaggta
     2221 gtagctttaa tgtcattaag gaagtgacct ttgatgatgc tctaccaacc ctgattatca
     2281 aggctcgtga ttttattcgt acccagctgc gtgagttcca atatctggat gacaatgggc
     2341 aatttcaaat tttgcctgaa tatccagaat ttgcctggtt tgaaggtgtt gtcaatgctg
     2401 ttactcaccg tgattactct gtctatggcg accatattcg tgttcttatg tttgatgacc
     2461 gactggaaat tcatagccca gggaaattgc caaatatcgt caccgttgac aatattaagc
     2521 atgaacggtt ttcaagaaat cctcggattg ccagaaccct gacggagttt ggatgggtcc
     2581 gtgagatgaa tgaaggtgtg aaacggattt actcagagat ggaatcagcc tttctccatg
     2641 aaccaaaata ctctgaacct gggaacaagg ttattctgac gcttgaaaat aatattgtca
     2701 gccgacacct acggacccaa gatagtttgg agaaacagtt tactgatttt ggagacctca
     2761 atgccgatga gcaattgttg atccattaca tgtacaattc aggagagaag atgacaactg
     2821 caaaagccat tgaactgaca ggaagaagtc gtaagtttat tgtaaaaacg atgaaaaaat
     2881 tacaagaatt gggtatggtt gactggtatg gtgccaataa aaatgacaga aatcaatact
     2941 atactttaac gaaaaattag cctatcctct aagag
//
LOCUS       DATRTN010000135         2915 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.136, whole genome shotgun sequence.
ACCESSION   DATRTN010000135 DATRTN010000000
VERSION     DATRTN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.136"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            1..246
                     /locus_tag="VBE22_001917"
     CDS             1..246
                     /locus_tag="VBE22_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HEO7203344.1"
                     /translation="MHTLGSVIELKNDSQKVMITSRFPLYDNEGQLGYFDYSGCIFPI
                     SIVGNETYFFNLEDIDKVLFEGYYDENEEEMQKIFLK"
     gene            373..801
                     /locus_tag="VBE22_001918"
     CDS             373..801
                     /locus_tag="VBE22_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203345.1"
                     /translation="MKKDKYIALCLLCAIATSNLSAVTVFAQDAQTILTQATTVTDQT
                     SEKAGENSSAEATSASESVSSEATTVVPEASLPSTDLHAQPADSKTAPTETVIEGQTE
                     TNPTTSPDKQFLQQVIPKEKHRLGRKQIQANPKLQLLVSL"
     gene            807..1616
                     /locus_tag="VBE22_001919"
     CDS             807..1616
                     /locus_tag="VBE22_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203346.1"
                     /translation="MVPSQPQVTATPQKSEVVTPAITSGIDLPDVAIPTAMASAAYVK
                     HWIGNDAYTHNLLSHRYGITAAQLDGFLQSTGITYDSSRIDGQKILDREKSSGLDARA
                     IIAIAIAESSLGTQGVATAPGANMFGFGAVDNNTTNAQNFSDDKAVIKMTQETIIQNQ
                     NTSFAIQDQKAQFLSTGNLNVAARGGVYFTDASGSGKRRAAIMESIDKWIDAHGGISE
                     ISKEPLNTSSVAMMAVPTSYSVSRANQAGNYVAGTYPWGQRTWLPVWTIYG"
     gene            1552..1869
                     /locus_tag="VBE22_001920"
     CDS             1552..1869
                     /locus_tag="VBE22_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO7203347.1"
                     /translation="MWQALTLGAKELGYQFGPFMGNGGDWKNKPGYQTTHEAKTGYAI
                     SFSPGQAGADRTYGHVAIVEDVKEDGSIPISESNVLGLGTISYRTFSAAEAAQLTYVV
                     GEK"
     gene            2264..2713
                     /locus_tag="VBE22_001921"
     CDS             2264..2713
                     /locus_tag="VBE22_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEO7203348.1"
                     /translation="MTEQQIEYINNRSEIAGHLEAIVSEIYLREESGKIESWFVTIPD
                     MNSFVAETNMDREQILFLLDSAHKYHIYFLFGGLASYLMTNISDVPKAIRTQAQYVLL
                     GMRVMDQSVLPKSYNSKEPYLKPDMIYIHDRRQERMLKITQEIEMEH"
     gene            2718..>2915
                     /locus_tag="VBE22_001922"
     CDS             2718..>2915
                     /locus_tag="VBE22_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203349.1"
                     /translation="MKKQKLLLLIGGLLIMIMMTACKDSKIPENRTKEEYQAEQNFKP
                     FFEFLAQKDKDLSKIQKYLLLV"
BASE COUNT          994 a          546 c          574 g          801 t
ORIGIN      
        1 atacatactc taggaagtgt cattgaatta aaaaatgata gtcagaaagt catgattaca
       61 tcaagatttc cgttatatga caatgaaggt caattgggat attttgatta ttctggatgt
      121 attttcccaa ttagtatagt tggaaatgaa acttattttt tcaatttgga agatatagat
      181 aaagtcttgt ttgaaggtta ctatgatgaa aatgaagagg agatgcaaaa aatatttttg
      241 aaataaataa aagatatcaa ataccctcac ttttcagtag acgttttaaa atagtcatct
      301 gaagaataaa agaaaagtaa tttttaatat taaattcttt aaacactcta taaactatta
      361 aggaaaaacg ttttgaaaaa agataaatat attgcattgt gtttgttgtg tgcaattgcc
      421 acgtcaaact tatcggctgt tacggttttt gctcaggatg cacaaactat tttgacgcaa
      481 gctacgactg taactgatca aacaagtgaa aaagctggag aaaattcatc agctgaggcg
      541 acaagcgctt ctgagtcagt ttcatcagaa gcgacgacag tggtacctga ggcaagtctg
      601 ccttcaactg atctccatgc ccaaccagca gatagcaaaa cagctcctac tgagactgta
      661 attgagggac aaacagaaac taatccgacg actagtccag ataaacagtt ccttcaacaa
      721 gtgattccaa aggagaaaca caggctggga cgcaaacaga tacaagcaaa cccgaaactc
      781 cagctcctag tcagtctgta acaccagtgg tgccaagcca acctcaagtg acagcaacac
      841 ctcagaaatc tgaagttgtg acaccagcca ttacgagtgg cattgatttg ccagatgttg
      901 ccatacctac ggcaatggct agcgcagctt atgtcaaaca ctggataggc aatgatgctt
      961 atacacacaa tctattgtct caccgttacg gcattactgc cgcacagctt gatggattct
     1021 tacagtcgac aggtatcaca tacgatagca gtcgcatcga cggtcaaaaa atcttagacc
     1081 gagagaaatc atctggtctg gatgcgcgtg caatcattgc cattgctatc gcagaaagtt
     1141 cgcttggtac acaaggcgtt gccacagcac caggtgctaa tatgtttggt tttggggcag
     1201 ttgacaacaa tacaactaac gctcaaaact ttagtgatga caaggctgtc attaaaatga
     1261 cgcaggaaac catcatccaa aaccaaaaca ccagctttgc cattcaagac caaaaagcac
     1321 aattcttgtc aactggcaac ttgaatgtag cggctcgagg tggggtttac ttcacggatg
     1381 ctagcggttc tggtaaacgt cgtgcggcca ttatggagag cattgacaag tggattgacg
     1441 cgcatggcgg aatttctgag atttcaaagg aaccgctcaa cacgtcgtcc gtcgctatga
     1501 tggcagtccc aacgagttac tctgtttcaa gagctaatca agcgggcaac tatgtggcag
     1561 gcacttaccc ttggggccaa agaacttggc taccagtttg gaccatttat gggtaatggt
     1621 ggcgattgga aaaataagcc gggctatcaa accacacacg aggcaaaaac aggctacgcc
     1681 atctcattta gccccggcca agctggtgct gatagaactt acggccacgt ggccatcgtc
     1741 gaagacgtta aggaagacgg atctatcccg atctccgaat caaatgtcct aggacttggc
     1801 accatctcct accgcacctt cagtgcagca gaagcagcac aattgactta tgtagttggg
     1861 gagaagtaga taattattct ttgaaaaaca aaaggttctt agcttgtcaa aatctaagac
     1921 ccttttatct ttcataagaa ctttaaaatc aacagatttt tatcaccaaa tggactggac
     1981 cgagaccaat agccattcca attgttccag aaacacttac ctttgaggag tttaaggcat
     2041 atgaggatgt taaaaaagca agtgagacta atcagatttc acttggtatt agcttaaaag
     2101 aagttgataa ttgtagcatt ccactttcga aattcaaaca gttacttttt acttctgaca
     2161 atccagataa tttgtctaac ttgatgcgct tcttagttaa atctgtttca ttgattaaag
     2221 atagtaagtt tatcttttag acactgcttc tgattatgat gatttgacag aacaacaaat
     2281 tgaatatatc aataatcgaa gtgaaatagc agggcattta gaagctattg tttcagaaat
     2341 ctatttaaga gaagaatctg gtaaaataga atcatggttt gttactattc ctgatatgaa
     2401 tagttttgtg gcagaaacaa atatggatag agaacagatt ttattcttac ttgactcggc
     2461 tcacaaatat catatttatt tcttatttgg tggtttagca tcatacctta tgactaatat
     2521 ctcagatgtt ccaaaagcta ttagaacaca ggcacagtat gttctcttag gaatgagagt
     2581 tatggatcag tcagtattac cgaaatcata taattcaaaa gaaccttatt tgaaaccaga
     2641 tatgatttat attcatgata gaagacaaga aagaatgctt aaaatcactc aagaaataga
     2701 aatggagcat tgagcatatg aaaaaacaaa aactattact gcttattgga ggcttattaa
     2761 taatgataat gatgacagca tgtaaggatt caaaaatccc agaaaaccgc acaaaggaag
     2821 agtaccaagc tgaacaaaat tttaaaccgt tttttgagtt tttagcacaa aaagataaag
     2881 atttgagcaa aatacaaaaa tacttactat tagta
//
LOCUS       DATRTN010000136         2836 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.137, whole genome shotgun sequence.
ACCESSION   DATRTN010000136 DATRTN010000000
VERSION     DATRTN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.137"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..764)
                     /locus_tag="VBE22_001923"
     CDS             complement(<1..764)
                     /locus_tag="VBE22_001923"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEO7203350.1"
                     /translation="MKFENIIYGIDGNVATIMLNRPDISNGFNIPMCQEIIDAIRLVS
                     ENKDVMFLVIEAQGPIFSIGGDLKVMKAAVESDDISSLTKIAELVNQISYDLLQLEKP
                     VVMCVDGAVAGAAANIALAADFVIASKKSKFIQAFVGVGLAPDAGGLLLLSKSIGITR
                     AVQLALTGESLSAEKAEALGIVYKLCESDKIGKIKDQLLKRLSRHSINSYQAIKSLAW
                     EAAFKDWEQYKKLELQLQESLAFKQDFKEGVRAHAD"
     gene            complement(901..1551)
                     /locus_tag="VBE22_001924"
     CDS             complement(901..1551)
                     /locus_tag="VBE22_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001900118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO7203351.1"
                     /translation="MCKYKAIIFDMDGVLFDTELFYYKRRERFLKQHGITIDHLPMNF
                     FIGGNMKQVWKSVLGDQYDTWDIDKLQQDYSRYKEDNPLPYKDLIFQDCKRVIEKLHH
                     KGYLLGLASSSTRHDIMLALESFNLDTYFKVILSGEEFSESKPNPAIYNRAAELLDIP
                     KQQILIVEDSEKGITAGIASGIDVWAIEDKYFGMNQSQANVLVSDLSQFFVKLDNY"
     gene            1645..2517
                     /locus_tag="VBE22_001925"
                     /pseudo
     CDS             1645..2517
                     /locus_tag="VBE22_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
     gene            complement(2759..2832)
                     /locus_tag="VBE22_001926"
     tRNA            complement(2759..2832)
                     /locus_tag="VBE22_001926"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2796..2798),aa:Asn,seq:gtt)
BASE COUNT          958 a          539 c          412 g          927 t
ORIGIN      
        1 ctatcagcgt gtgcccgaac accttcttta aaatcttgct taaaggctaa tgattcttgt
       61 aactgtaatt caagcttttt atattgttcc caatctttaa aagcagcttc ccaagctaat
      121 gattttatgg cttgatatga gtttatagaa tgtctactta aacgctttaa tagttgatcc
      181 ttaattttac ctattttatc tgattcacaa agtttataaa caattcccaa agcttcagct
      241 ttttcagcag ataagctttc tccagttaaa gctagttgca ctgcacgagt tataccgatt
      301 gatttactaa gtaacaataa acctcctgca tctggagcta aaccgacacc cacaaaagcc
      361 tggataaatt tactcttctt gcttgcgata acaaaatcag ctgctaatgc aatattagct
      421 gcggcaccag ctacagcccc atctacacac ataacaacag gtttctctag ctgtagtaaa
      481 tcatacgata tctgattaac taactcagca attttagtta atgagctaat atcatcgctc
      541 tcaacagcag ctttcatcac ttttaaatct cctccgattg aaaagattgg tccttgtgct
      601 tcaatcacta aaaacatgac atctttattt tcagacacaa gccttattgc atcaataatt
      661 tcttgacaca ttgggatgtt aaaaccattt gaaatatctg gtcgattaag cataatagtg
      721 gccacgttgc catcaatacc atatattata ttttcgaact tcaaaatgtc tcctctcttt
      781 aagagtgaaa gaaaaataaa aaataatttg attatcaaat tatcatgctt tgtattttat
      841 cataaaccta gctttcatgg caagataaaa aataaaaaaa cggaattaaa cttcccgttt
      901 ttaatagtta tctaatttca caaaaaattg actcagatcg ctcactaata cattagcctg
      961 cgactgattc attccaaagt atttatcttc aatagcccac acatcaatac cggatgcaat
     1021 accagctgta atacccttct cactgtcttc tacaataaga atttgctgtt taggaatatc
     1081 taaaagttct gctgctctat tataaatagc tggattaggt ttactttctg aaaattcctc
     1141 accagacaga attaccttaa aatatgtatc cagattaaaa ctttctaatg ctaacatgat
     1201 atcatgtcta gtagaacttg aagctagacc caaaagatag cctttgtgat gaagcttttc
     1261 aatgactctt ttacaatcct gaaaaattaa atctttgtat ggcaagggat tatcctcttt
     1321 atagcgacta taatcttgtt ggagcttatc aatatcccag gtatcatatt gatcacctaa
     1381 aacagatttc caaacctgct tcatatttcc tccaataaaa aaattcattg gtaaatggtc
     1441 aattgtaatt ccatgttgtt ttaaaaaacg ctctcgcctt ttataataaa ataattcagt
     1501 gtcaaataga acaccatcca tatcaaatat tattgcttta tatttacaca tgtaactatt
     1561 atatcattct tttaaattta gaggtaatga caaattttct gacttttgtt ataatttatc
     1621 taaaattcat tcttgaggta acctatgaaa gtagtcaaat ttggaggaag ttctttagca
     1681 tcttcccaac aattatacaa agtactaaat attatcaaat cagactacac tagacggttt
     1741 gttgtcgttt cagctccagg gaaacgctac gaagaagact taaaaatgac agatgctttg
     1801 atacaatatt atcaaaatta tattaacggt aaagatattg ttaaagacca aacttggatt
     1861 atcaatcgtt atcaagaaat tattagtgat ttgtctttag gaagcactat tgcagaagaa
     1921 attactcgct ctatagagca gctagcttca cttcccatag aaaataatca gtttttatat
     1981 gattgttttt tagcagccgg tgaagataac aacgcaaagt tagttgcaac gttttttaat
     2041 caaaatgaca ttcctgcaag atatgttcat ccaaacgaag caggaattat tgtaactaaa
     2101 gaaccatgta atgcacgaat tattccagga agttatgata agattgagaa cttatgtcta
     2161 tacaatgagg ttcttgttat ccctggattt tttggagtca cagaagataa ccaaatttgt
     2221 accttttcaa gagggggatc tgacattact ggatccctaa ttgcagcagg cataaaagca
     2281 gacctttatg agaacttcac agatgttgat ggtatatttg cagcacatcc aggtgtagtt
     2341 aagaaccctc acgctatccc tgagcttact tataaagaaa tgcgtgaatt agcctatgcg
     2401 ggtttttcgg ttttacatga tgaagcttta cttcctgcct atcgtggcag aatccctctt
     2461 gttattaaaa atacaaataa tccccaacag cctggtacaa aaatagtttt aaagcactat
     2521 atgaaacatt cttccaaaaa tagtacctat tacactactt acactattag atagataaca
     2581 aatcgtccta agtaagttta cttaggacga ttttatttag aacataggat agttttttca
     2641 attttaatcg taactacttg gtatcactga caaattcgga caattaagat gctagccaac
     2701 cttaaggaat aataattcca ataaaaaaag gctaaccaaa gttagtctcc tttatctact
     2761 ccgccagtag gactcgaacc tacgacatca tgattaacag tcatgcgcta ctaccaactg
     2821 agctatggcg gattat
//
LOCUS       DATRTN010000137         2755 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.138, whole genome shotgun sequence.
ACCESSION   DATRTN010000137 DATRTN010000000
VERSION     DATRTN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.138"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..64
                     /locus_tag="VBE22_001927"
     CDS             <1..64
                     /locus_tag="VBE22_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO7203352.1"
                     /translation="TFKKIVGISPLEYKQKPKTI"
     gene            complement(259..783)
                     /locus_tag="VBE22_001928"
     CDS             complement(259..783)
                     /locus_tag="VBE22_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO7203353.1"
                     /translation="MVKDRQIQKTKVAIYNAFISLLQENDYSKITVQDVIGLANVGRS
                     TFYSHYESKEVLLKELCEDLFHHLFKQGRDVTFEEYLVHILKHFEQNQDSIATLLLSD
                     DPYFLLRFRSELEHDVYPRLREEYITKVDIPEDFLKQFLLSSFIETLKWWLHQRQKMT
                     VEDLLKYYLTMVER"
     gene            915..1779
                     /locus_tag="VBE22_001929"
                     /pseudo
     CDS             915..1779
                     /locus_tag="VBE22_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
     gene            1864..2706
                     /locus_tag="VBE22_001930"
                     /pseudo
     CDS             1864..2706
                     /locus_tag="VBE22_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631788.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
BASE COUNT          941 a          453 c          433 g          928 t
ORIGIN      
        1 gacatttaaa aaaatagtgg gaattagccc actagaatat aagcaaaagc ctaagacaat
       61 atagtaatcg aaaaatattc attttgctta tggtgtcatc aacaatccca aagaaactct
      121 ttcatcaagg ggactttttt atctcccgaa ttatactcaa ttaatctaat aacttcaact
      181 cgctatatta gacaatcaat ccattcatca cctctgataa cgctaatgaa ataagagaat
      241 ctcccaactc tctataattt acctctctac catagttaaa taatatttta gtaaatcttc
      301 aacagtcatt ttctgtcttt gatgaagcca ccactttaag gtctcgataa aactggataa
      361 aagaaattgt tttaagaaat cttcaggaat gtccaccttt gtaatatact cctcacgcaa
      421 tctagggtaa acatcgtgtt ctaattcact tctaaagcgt aataaaaaat agggatcgtc
      481 agataataat aacgtagcga tactatcttg attttgttca aaatgtttta agatatgaac
      541 taaatactct tcaaacgtaa catctctacc ctgcttaaat aagtggtgaa aaaggtcttc
      601 acagagctcc ttgagtaaca cttctttgct ttcatagtga gagtaaaaag ttgatcttcc
      661 tacatttgca agtccgatta catcttgaac cgttatttta gaataatcat tttcctgtag
      721 caagcttata aaagcattat aaatcgctac ttttgttttc tgaatttgtc tatcttttac
      781 catataggac actttctaag ttttgttcag tattgaacaa gtgttctttt ttgtttctag
      841 caattctctt ttatctccta gataatagaa gaaagaatca cactacaata ccagtataac
      901 aaaaggagaa aaatatgaca tcaaacaaaa aagtggctat cgcttttata ttaaatatca
      961 gcttttccgt ccttgaattc atctttggaa gtctattctt ctcaggtgct atcttagccg
     1021 atgctgttca tgattttggt gatgcaatag ctattggcat ctcagctact ttagaaaaaa
     1081 aaatctaaga aagatgaaga tacgatattt tcattaggtt ataaacgttt tagcctttta
     1141 ggagctctta tcacaagtct aattcttata agtggatcta tcttagtcat gatagaaaat
     1201 attccaaaat tgtggcatcc tactcctgtt aattatcacg gcatgtttat tctagctgtc
     1261 atcgctatta tcattaatgg tctagctagt tttattctcc attctggtca aagtaaacat
     1321 gaggaaatat taagccttca ttttttggaa gatattctag ggtggttagc tattattgtt
     1381 atatctctta tcttaaattg gaaaccgttg tatatccttg atccattatt atcagttgcc
     1441 atatctactt tcatcttatc taaagcactt cctaaattac tatcaaccct gaaacttttt
     1501 ttagatggtg ttccagattc catagattac gctgcattac atgatgaact taagggactt
     1561 tcgcaagtaa gatctatcaa tcaactcaat atctggtcaa tggatggtat tgataatcga
     1621 gcaatcattc actgttgctt aaatcaattg atatctgaaa aagattgtaa aagagctatt
     1681 cgaacgattt gccaacatta taagataaac gatgttactg ttgaaatcga ttattcttta
     1741 agagaacatc aaaatcattg caaaccatta aaaaactaac ctgagtgtta gttttttcct
     1801 tgacttgaag ttaactctaa gtgctaagat taggtcagta ctaatgaaag aaggaaaata
     1861 aagatgacag atacaatgat attaaatgac aatcataaaa taccaacttt aggtttaggt
     1921 acgtggatga tagataatga tagggtagct caagttgtta aagatgctat cgaattaggt
     1981 taccgccaca ttaatactgc acaagcatac aacaatgaaa aaggtgtcgg tgtagggatt
     2041 aaagaatctg gtattacacg tgaatcttta tttgttacga ctaaaattgc cgctgaacat
     2101 aaagattatg aaagtgctac aaaatcaatt gatcaatcac tagaagcttt agggcttgat
     2161 tgtattgatt taatgattat tcatagtcca cagccttgga aggaatgacg tgaaacagat
     2221 aaacattttg accaaggaaa tttagaagct tggagagctt tagaggatgc tcaaaaatct
     2281 ggtaaggtaa aatccatcgg tgtctcaaac tttttagaaa aagacttaaa aaatggtcat
     2341 gttaaacctg ctgtcaatca aatcttagct cacatcggaa atacaccttt cgacttgatt
     2401 gattactgtc aaagtaaagg tattcaagtt gaagcttatt caccaattgc acatggacaa
     2461 gcattgaaat ctgatggtat tcaaaaaatg gctgaaaaat atggggttag tgttgctcaa
     2521 ttatgtattc aatatttact acaattaaac ctgatcgttt taccaaaggc ttctagtaaa
     2581 gaacatttac agtctaatct agactttgac tttgtaatta gtgacgaaga tatgtcgata
     2641 ttaaaatcac ttatgtttga cgattatggg gaattttcta acttccctgt ttttagtgaa
     2701 aaatgaaacc atatgcggat ttttacagaa gtttaatttt caaaattttt aattt
//
LOCUS       DATRTN010000138         2747 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.139, whole genome shotgun sequence.
ACCESSION   DATRTN010000138 DATRTN010000000
VERSION     DATRTN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.139"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            5..1336
                     /gene="rlmD"
                     /locus_tag="VBE22_001931"
     CDS             5..1336
                     /gene="rlmD"
                     /locus_tag="VBE22_001931"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO7203354.1"
                     /translation="MPLKIKRMGINGEGIGFYKKTLIFVPGALKGEEVFCQISSVRRN
                     FAEAKLLKINKKSKNRVDPACSIYKECGGCQIMHLQYDKQLEFKTDVIRQALMKFKPE
                     GYENYEIRKTIGMPEPEHYRAKLQFQVRSFGGNVRAGLYAQGTHRLIDIKDCLVQDRL
                     TQEMINRVAELLGKYKLPIYNERKIAGVRTVMIRRAQASGEIQLIFITSKRLDFDDVV
                     IELVREFPELKTVAVNINASKTSDIYGQITEVIWGQESINEEVLDYGFSLSPRAFYQL
                     NPKQTQILYSEAVNALDVKEDDDLIDAYCGVGTIGLAFAGKVKSVRGMDIIPEAIQDA
                     KENALHMGFTNTHYEAGKAEDIIPRWYSEGFRANALIVDPPRTGLDDKLLNTILKMPP
                     EKMVYVSCNTSTLARDLVTLTKVYHVHYIQSVDMFPHTARTEAVVKLQRKE"
     gene            1604..2368
                     /locus_tag="VBE22_001932"
     CDS             1604..2368
                     /locus_tag="VBE22_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEO7203355.1"
                     /translation="MIINSFDNSRKAIINPEDINSPIKGFPKTVITCFARETFNRILE
                     ELPHREIARTSVANLEIPIYELEFKGQKIGFFNAYVGASACVAILEDIIVFGMENLIV
                     FGTCGVLDSSIEETSIIIPRSAIRDEGTSFHYSEASSEIAVNTKFHEEFKSFLDRQAV
                     SYTEGKVWTTDGIYRETVDKMLEHKRNGAICVDMECSAIAALSKFRNINHFQFFYSAD
                     NLDAEVWEARSLGNSEDLEKKDKIAYIAMEFAIETF"
     gene            2402..2713
                     /locus_tag="VBE22_001933"
                     /pseudo
     CDS             2402..2713
                     /locus_tag="VBE22_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768361.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          943 a          352 c          571 g          881 t
ORIGIN      
        1 gcgaattcct ttaaaaatta aacgaatggg aattaatggt gaaggaatag gtttttataa
       61 aaaaacacta atctttgttc caggggctct taagggagaa gaggtgtttt gtcagatttc
      121 ttctgttaga cgtaattttg cagaagctaa actgttaaag ataaataaaa agtctaaaaa
      181 tagggtagat cccgcttgtt ctatttataa agaatgtggg ggatgccaaa taatgcacct
      241 tcaatatgat aagcagctag agtttaaaac ggatgttatt cgacaggctc taatgaaatt
      301 taaaccagag ggatatgaga attatgaaat ccgtaaaacg ataggtatgc cggaacctga
      361 acactatcgt gctaaattac aatttcaagt gcgttcgttt ggagggaatg tgagggcagg
      421 tttatatgca caagggacac atcgattgat tgatatcaaa gattgtttag tgcaagatag
      481 gcttactcag gaaatgataa atcgagttgc agaactttta ggaaaatata aattgcctat
      541 ttataatgaa cgtaaaatag ctggtgtccg tactgttatg attcgacgag cacaagcgag
      601 tggagagatt caactgatct ttatcacaag taaaagactt gattttgatg atgttgttat
      661 tgaattagtt cgagagtttc ctgagttaaa gacagttgct gtaaatatca acgctagtaa
      721 aacaagtgat atctatggtc aaataacaga agttatttgg ggtcaagaat ctatcaatga
      781 agaagtatta gattatggtt tttcgttatc accacgtgct ttttatcaac ttaatcctaa
      841 acaaacacaa attttatata gtgaggcagt taatgcctta gatgtgaagg aagatgatga
      901 tttaattgac gcttattgtg gagtaggaac gattggactt gcttttgctg gaaaagttaa
      961 aagtgttcgt ggaatggata ttatccctga ggctattcag gatgctaaag agaacgcact
     1021 tcatatgggt tttactaata cacattacga agctggaaag gcagaggata ttatccctag
     1081 gtggtatagt gagggatttc gcgctaatgc gttaatcgtg gatcctcctc gcacaggttt
     1141 agatgataaa ttgctaaata ctatactaaa aatgcctcca gaaaaaatgg tttacgtttc
     1201 ttgtaataca tccacgcttg ctagagactt agtaacatta acaaaagtat accatgtcca
     1261 ctacatccaa tccgttgaca tgtttccaca tacagcaagg accgaggctg tagtgaaact
     1321 acaacgtaag gaataataaa gttttctaat caaaaaataa gagtattttt gggtattctt
     1381 gtttttagat agtaatggga gatattaata taacatagta taaaaacaca tggtttaagc
     1441 tatttgatac ggaaatttac cttagaaatg gtaaatagtt ccattaagta tattcatctt
     1501 tgcaaatttg accttgcctt ttaagtttca ttgagttaaa atagaaaaaa tatttttaac
     1561 aagatgatag aacatcgtaa attaaggttt aaaggaaatg cttatgataa taaattcatt
     1621 cgataatagt agaaaagcga ttattaatcc tgaagatatt aattcaccaa taaaaggttt
     1681 tccaaagact gttattacct gttttgcgcg ggagactttc aatcgtattt tagaagaatt
     1741 gcctcatcgt gaaattgcta gaactagtgt agcaaactta gaaattccca tttatgaatt
     1801 agaatttaaa ggacaaaaga ttggcttttt caatgcttat gttggtgctt cagcttgcgt
     1861 agcaatcttg gaagatatta tagtgtttgg aatggaaaat ttaattgttt ttggaacatg
     1921 tggcgtcctt gattcgtcaa ttgaggagac atccattatt attccaagaa gtgctattag
     1981 ggatgaaggg acatcatttc attattcgga agcaagttct gaaattgcag taaacacaaa
     2041 atttcatgaa gagtttaaat cttttttaga tcgtcaagct gtttcttaca ctgaaggaaa
     2101 agtttggaca acggatggta tttaccgtga gacagttgat aaaatgttgg aacataaacg
     2161 gaatggagca atctgtgtgg atatggaatg ttcagcaatt gcagctttat ctaagtttcg
     2221 aaatattaat cattttcaat ttttttactc tgcggataat ttagatgcag aagtatggga
     2281 agcaagatct ttaggaaata gcgaggattt agaaaagaaa gataaaattg cttatattgc
     2341 catggagttt gctatagaga ctttttaggg tctactgtat gatgttgaaa ggaagatagc
     2401 aatgtggaat gtaaaaactt ttaataattt aactacacat gagttgtttc aaatatataa
     2461 attaagagta tctgtatttg tggttgaaca agattgtcct tatcaagagg ttgatgatga
     2521 agatttgatt tgtctgcatg ggatgaattg ggtagatggt caattggcgg cttattatag
     2581 attgatacca gaagatgata aggtgcattt gggacgtgtg attgttaatc cagactttag
     2641 aaaaaaggga ttaggaaatc aattagtgga atatgcgatt aaatttagtg aggcaaatta
     2701 ccctaataaa cctaatatgg ttttatcata aatacatact atttaaa
//
LOCUS       DATRTN010000139         2639 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.140, whole genome shotgun sequence.
ACCESSION   DATRTN010000139 DATRTN010000000
VERSION     DATRTN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.140"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            85..1227
                     /locus_tag="VBE22_001934"
     CDS             85..1227
                     /locus_tag="VBE22_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEO7203356.1"
                     /translation="MKVVVAIDSLKGSLSSLEAGNAIKESINEVIPGADVEVHPLADG
                     GEGTVEALTLGMGGTIETIPVKGPIGEKVHASYGIIPQRQLAIIEMAAAAGITLIATE
                     ERNPLHTTTYGVGEMIKDAISKGCRHFIIGIGGSATNDGGAGMLQALGYALLDKDNQE
                     ISLGAQGLADLKSISTDKVIEELKECDFKIACDVTNPLCGAQGCSSIFGPQKGADEDM
                     ITKMDTWLSNYATLATSVSEKADATIEGTGAAGGLGFAFLAFTNATLEPGIDIILSEI
                     NIEKAISVADLVVTGEGRLDGQTVMGKAPIGVAKLAKKYGKKVVAFSGSVTEDAILCN
                     QHGIDAFFPIVRRLISLDEAMSKEVAYKNMKETATQVFRLINLYNH"
     gene            1252..2508
                     /locus_tag="VBE22_001935"
     CDS             1252..2508
                     /locus_tag="VBE22_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEO7203357.1"
                     /translation="MEATFTTTGALIGLALAILLIIKKVHPAYSLILGALVGGLIGGG
                     DLVTIVNTMVLGAQGMMSSILRILTSGILAGALIKTGSAEKIAESIIKKLGQERAITA
                     LAIATMIICAVGVFIDIAVITVAPIALAIGKKANLSKSSILLAMIGGGKAGNIISPNP
                     NTIAASEAFKVDLTSLMVQNIIPAIAALVVTIILAKIVSKKNNDISYDSEEQVGSDLP
                     AFLPAISGPLVVICLLALRPLFGITIDPLIALPLGGLISILATGYLKETVPFVEYGLS
                     KVVGVSILLIGTGTLSGIIKASNLQFDMIHLLEFLNMPTFILAPLSGIFMGAATASTT
                     SGTTIASQTFAETLIKSGVPAVSGAAMIHAGATVLDSLPHGSFFHATGGAVNMAIKDR
                     MKLISYEALIGLTSTIVAVVYYCFFG"
BASE COUNT          808 a          401 c          576 g          854 t
ORIGIN      
        1 ttgattttgt tactataatt gagtgatata aaatagtgaa aaaaataaca aagattcaca
       61 aaatattttc aaggaggttt tcaaatgaag gtagtagtag ctattgattc tttgaaaggg
      121 agtctttctt ctttagaagc tggcaatgct ataaaggagt caattaatga agtaatccca
      181 ggggcggatg tggaagtaca tccattagca gacgggggag aaggaactgt tgaagctttg
      241 acgctaggta tgggggggac gatcgaaaca atccctgtta aggggcctat tggagaaaaa
      301 gtacatgcga gttatggtat tattccacag cgtcagttag ctattattga aatggctgca
      361 gctgctggta ttaccctaat tgctactgaa gagagaaatc ctctccatac aaccacttat
      421 ggagttggag aaatgataaa ggatgctata agtaaaggct gtaggcactt tattattggt
      481 attggaggta gtgctactaa tgatggtggg gcaggtatgc tacaagcttt gggatatgca
      541 ttattagata aagataatca agaaatcagt ttgggagcac aaggattagc agatttaaaa
      601 tctatctcta ctgataaggt tattgaggaa ttgaaagaat gcgattttaa aattgcatgt
      661 gatgtaacaa atcctttatg tggtgctcaa ggttgtagtt cgatttttgg accgcaaaaa
      721 ggtgctgatg aagatatgat tacaaaaatg gatacttggt taagtaatta tgctacctta
      781 gctacttcag ttagtgaaaa agcagatgca actattgaag gaacaggtgc agcgggtggg
      841 cttggatttg cctttttagc ctttacaaat gctaccttag aacctggtat tgatattatt
      901 ctatcagaaa ttaacataga aaaagcgatt tctgtagctg atttagtagt tactggtgaa
      961 ggtcgtctgg atgggcaaac ggtgatggga aaagctccaa ttggcgtagc aaaattagct
     1021 aaaaaatatg gtaagaaagt agtggcattc tctggatctg tcaccgaaga tgctatactt
     1081 tgtaatcaac atggtattga tgcttttttt ccaatcgtac gacgccttat cagtttagat
     1141 gaggctatgt ctaaagaggt agcttataaa aatatgaaag aaactgctac tcaagtcttt
     1201 agattaatca atttgtataa tcattaatat tgaaaggaat tctgataagt tatggaagct
     1261 acatttacaa caacaggggc tttaatagga ttagcgcttg ctattttact gattatcaaa
     1321 aaagttcatc ctgcctatag tctcattctt ggagcattag ttggaggttt gattggtggc
     1381 ggtgatttag ttacaattgt caatactatg gtattaggtg cgcaagggat gatgtcatcc
     1441 atattacgca ttttaacatc agggatttta gcgggtgctt taattaaaac cggatcagct
     1501 gaaaaaattg cagaaagtat cattaaaaaa ttaggacaag agcgagccat cacagcttta
     1561 gccatcgcaa caatgattat ttgtgcagta ggtgtcttta ttgatattgc agttattaca
     1621 gttgctccga ttgcattagc aataggtaaa aaagctaatc tttctaaatc tagcatactg
     1681 ttagcaatga ttggtggtgg taaggctggg aatattatct cacccaatcc taatacaatt
     1741 gctgcttcag aagcatttaa ggttgattta acaagcttaa tggtacaaaa tattattcct
     1801 gcaattgcag ctttagtagt tacgattata ttagcaaaga ttgtttccaa gaaaaataac
     1861 gatatttctt atgatagtga ggagcaagtg ggctctgatc tgccagcatt cttaccggct
     1921 atttctggtc cacttgtggt tatctgctta ttagctcttc gtcctttatt tggaatcact
     1981 attgatccct tgattgcact gccgttgggt ggtctaatta gtattttagc aacaggttac
     2041 ctaaaagaaa cggtaccgtt tgttgaatac ggtttatcaa aagttgttgg cgtttcaatt
     2101 cttttgattg gaacaggtac actctcaggt attattaaag catctaatct acaatttgat
     2161 atgattcatc tccttgaatt tttaaatatg ccgaccttta tccttgcccc actctcaggt
     2221 atttttatgg gagcagcaac agcatcaacg acttctggaa caactatcgc ttcacaaacg
     2281 tttgctgaaa ccttgattaa atcgggggta ccggcggttt cgggtgcagc tatgattcac
     2341 gcaggtgcga ctgttctgga ttcgctacct catggatcct tcttccatgc gacaggcgga
     2401 gctgtcaata tggctataaa agatcgtatg aaactaatta gctatgaagc tttgattgga
     2461 ttaactagca caatagttgc tgtcgtttac tattgtttct ttgggtaaaa tttggatgaa
     2521 tacgtgatgt ttattatcac tgtttgaata gaattctcat tgattttttc taattttctt
     2581 actataataa attagtagga gaggtgttgt gaaacaaaca aagaaagaat ttattttag
//
LOCUS       DATRTN010000140         2568 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.141, whole genome shotgun sequence.
ACCESSION   DATRTN010000140 DATRTN010000000
VERSION     DATRTN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.141"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(218..982)
                     /locus_tag="VBE22_001936"
     CDS             complement(218..982)
                     /locus_tag="VBE22_001936"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEO7203358.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGVLDYNEETAQAAVD
                     KLSPEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGIAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            1150..1848
                     /locus_tag="VBE22_001937"
     CDS             1150..1848
                     /locus_tag="VBE22_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001907086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO7203359.1"
                     /translation="MPFIFRHSFFNKVLIFWYTIIMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDTFDNPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIINVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPSHYSVHPGHGMN
                     TTIGHEKNFNPFFQ"
     gene            1845..2555
                     /locus_tag="VBE22_001938"
     CDS             1845..2555
                     /locus_tag="VBE22_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO7203360.1"
                     /translation="MNIKRHSIFLWLTTSILAILLAIGMTIYFNTYQASPLARRIAKT
                     ATNHKNYLLFRSKGKVKANIIFYQGALVEEEAYSQLARDLADKGYNTYILKTPLNLPV
                     LSPYKAKTIIDQNHLTNVYLAGHSLGGVVASQNAKVAPVRGLILLASYPSSKSDLSHK
                     KLRVLSITASNDHILNWEKYEEAKKRLPNSSTFRTIVGGNHSRFGNYGHQKGDGKATL
                     SHKSSEKQLATFISNFIH"
BASE COUNT          798 a          551 c          401 g          818 t
ORIGIN      
        1 gcattgggac ctgctccagt agcctccaag tcaactactg catattttct aagttttttc
       61 tgggtcatag taagtctatt atagcacgct atatctataa aacaaaacat gactgtacct
      121 caaagatgcc atcactccaa taaccaagat aagataattt tttaagaaat ataaaaaaca
      181 ctgtgagttt cactcacagt gttagcatta atcattttta atggaatacc ataccaccat
      241 cgacaactat tgtttgtcca gtgatataat ttgaatcatc tcccgctaag aagcctactg
      301 cattagcaac atcttctggt tcagacaagc gtttcaaagt gatatctttt gcaaactgct
      361 ccatacccca ttcatcatct ttgcctgcat tcttaccaac ttcatgggca atatcaaaca
      421 tcataggtgt tttaacaata cctggtgcgt aggcattgac tgtgattcct tctgatgcca
      481 aatcctttgc caaagtctgt gtaataccac gaactgcaaa ttttgtacca ccataaacag
      541 tcaaattagg attcccttca catcctgctt gtgaagttgc atttataatt tttccgccat
      601 gacctaattc tctaaaatgt ttttgagctg cttgacttcc ccaaatagtt cctccgacat
      661 tgattgcaaa agccttttca aactgctctt cagtaatagt gtcaagaggg gttgttgggg
      721 caatacctgc attgttaaca acaacattca aatcaccaaa agtatctaca actttttgga
      781 aagcatcaaa gacttgatca cgttttgaaa catctgctac aactgcaaca gcatcctcag
      841 gtgatagttt atcaaccgca gcctgagcag tctcttcatt atagtctaaa acgcctattt
      901 taaagccgtc tgcatgaaga cgtttcgcaa tagcaaatcc aattccttga ccagcaccag
      961 taacaatagc aacttttgac atatcttcca cctcatattc tttagtcaac caattaggtt
     1021 gctctcaaaa agtcagtgtt cttaaactaa gttcagcttt aggcttggct tattgattac
     1081 ttttatgata cacctttatg aaaacggtgt caagaaattg ctgtgagatc ccatattttc
     1141 tgaaactaat tgccatttat tttccgccac tcttttttta acaaagtctt gattttttgg
     1201 tacactatca ttatgaaaat ctataaaaca ataaaccaca ttgctggtga aaatacttat
     1261 tacctcgtta atgatcaagc tgttatcctc attgatccag ggagcaatgg ccaagaaatc
     1321 attgctaaaa ttaaatcctt tgaaaaacct ttagtggcta tcctcttaac tcatactcac
     1381 tatgatcata tttttagttt agatttggtc agagatactt ttgataatcc acctgtttat
     1441 gtttcagaaa aagaagccgc ttggctatct tccccagatg ataatttatc tgggttaggc
     1501 cgtcatgatg atatcattaa tgttatagca agaccggcag agaacttttt taaattaaag
     1561 caaccctatc aactaaatgg ttttgaattc acagttttac caacacctgg tcattcttgg
     1621 ggaggagtct catttgtttt ccactcagat gaattagtcg ttactggcga tgctctattt
     1681 cgtgaaacta tcggtcgtac tgacttacct accagtaact ttgaggacct cattactggt
     1741 attcgccaag aactattcac cttacctagt cattatagtg ttcacccagg ccatggcatg
     1801 aatacaacta ttggccacga aaaaaacttt aatcctttct tccaatgaac ataaaacgtc
     1861 actctatttt tctttggtta acaacaagta tccttgctat tttactggcc atcgggatga
     1921 ccatttactt caatacttac caagcctccc cattagctag gagaattgct aaaaccgcta
     1981 caaaccataa gaactacctt ctttttagaa gcaagggtaa ggtaaaagca aatattattt
     2041 tctatcaagg ggctcttgtg gaagaagaag cttattctca acttgctaga gatttagctg
     2101 ataaagggta taatacctat attttgaaga cacctttaaa tttgccagtt ttatcacctt
     2161 ataaagctaa aacaattatt gatcagaacc acttaactaa tgtttacctt gctggacatt
     2221 ctttaggagg cgttgttgct agccaaaatg ccaaagtagc ccctgttaga gggctcatat
     2281 tattagcttc ctacccttca agtaaatccg acctatctca taaaaagtta agagtactct
     2341 ccattacagc aagtaacgat catattttaa actgggaaaa atatgaggaa gctaaaaaac
     2401 gcctcccaaa ttcaagtact tttcgaacta ttgtcggggg aaaccatagc cgatttggta
     2461 actacggaca tcaaaaaggg gatggtaaag ctaccctttc tcacaaatca tctgaaaaac
     2521 aattggctac gtttatttcg aattttatcc actagcatga tatctaca
//
LOCUS       DATRTN010000141         2507 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.142, whole genome shotgun sequence.
ACCESSION   DATRTN010000141 DATRTN010000000
VERSION     DATRTN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.142"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..736)
                     /locus_tag="VBE22_001939"
     CDS             complement(<1..736)
                     /locus_tag="VBE22_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEO7203361.1"
                     /translation="MILKICRAAYSLQWGGVYQLALLDYPRIKAFELERIGAFIAYEK
                     QYKRKIEIQCDDKHLLTKIVHFLKYNSFTFPYIPKYREAAATFNEDGISLTSDFLSHT
                     CTIETAKLIFKEGKILSAVKAFNKPAEVLVNDKRNAAGDPKDYFDYVMLNWSNTNSGY
                     RLVMERLLGKAPSEQELTVAFKPGVSFHFNYQDIINHPDSIFDGYHPAKIKNQLSLAE
                     HLVACVIPKHYQEDYQSLVPNDLKHRV"
     gene            complement(900..1673)
                     /gene="fetB"
                     /locus_tag="VBE22_001940"
     CDS             complement(900..1673)
                     /gene="fetB"
                     /locus_tag="VBE22_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEO7203362.1"
                     /translation="MSGANSISLMSLLMASSLVLITLFFSYWQKLNLEKEVIISAIRA
                     VIQLLAVGFLLDYIFGYQNPIFTALLMLFMIINASYNAAKRGKGINKGFVISFIAIGS
                     GTIITLSVLIFSGILKFVPNQMIPVGGMIISNSMVAIGLCYKQLLSEFRSKQEEVETK
                     LALGADILPASIDIIRDVIKTGMVPTIDSAKTLGIVSLPGMMTGLILAGTSPIQAVKY
                     QMMVTFMLLATTSIASFVATYLAYKIFFNNRKQLVVTKD"
     gene            complement(1670..2326)
                     /locus_tag="VBE22_001941"
     CDS             complement(1670..2326)
                     /locus_tag="VBE22_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO7203363.1"
                     /translation="MSYLTFKHVDFKTDDKLVLNDINFAIDEGDFVSIVGPSGSGKST
                     VLKLASGLMSPTAGHIFFDGKDLNQLEPIESRKMISYCFQTPHLFGNTVEDNISFPYH
                     IRHEKVDYRRVDDLFQRFEMDQSYLKQDVKKLSGGEKQRIALIRQLLFEPKVLLLDEV
                     TSALDNHNKAIVEKVIKSLHDKGITILWITHDEEQSRRFANKVLKVVNGSIEAMEVIK
                     "
BASE COUNT          881 a          462 c          339 g          825 t
ORIGIN      
        1 aaactctgtg tttcaagtca ttaggcacaa ggctttgata atcttcttga taatgttttg
       61 ggataacaca tgcaactaaa tgttctgcta aagaaagttg atttttaatt ttagcaggat
      121 gataaccatc aaaaatagaa tcaggatgat tgatgatatc ttgataatta aaatgaaagc
      181 tgacccctgg cttaaaagct actgttaact cctgttcaga tggtgctttg cctaacaatc
      241 tttccattac taaacgataa ccagaattgg tatttgacca gttcaacatc acatagtcaa
      301 agtaatcttt agggtctcca gcagcattcc tcttatcatt taccagtact tcagcaggct
      361 tattaaaggc tttaactgct gataagattt taccttcttt aaaaattagt tttgcagttt
      421 caatcgtaca tgtatggctt aaaaaatcag aagttaaact aataccatcc tcattaaaag
      481 tagctgccgc ttctctatat ttgggaatat agggaaaagt aaaactattg tattttaaaa
      541 aatgaacaat ttttgtgagg agatgtttat cgtcacattg tatctcaatt tttcttttat
      601 attgtttctc gtaagctatg aaagctccta tcctttccaa ttcaaacgcc ttaattcgag
      661 gataatccag caaagctaat tggtaaacac ctccccattg taaactatat gctgcacgac
      721 aaatttttaa aatcatttct tcctcctttt gataatccct ataacaattt ctattaaata
      781 gtagcattta aacgtaatcg actcaataac ttagtcaatt ttcgatcaaa aataagatta
      841 agataccaaa aaagcctgga ctatacttac cagactaaca tactgtttat taaaatgaat
      901 caatcttttg ttactactaa ttgcttacga ttattaaaga aaattttata agccaaataa
      961 gtagctacaa aagaagctat cgaagtcgtt gctaatagca taaacgtcac catcatttgg
     1021 tatttaaccg cttggatagg tgaagttcca gcaagaatta accctgtcat catccctggc
     1081 aaagatacaa tcccaagtgt tttagctgaa tcaattgtag gtaccatacc ggttttgata
     1141 acatcacgaa taatatcaat cgaagctggc aaaatatctg ctcctaaagc taattttgtc
     1201 tccacttcct cttgtttact tctaaattcg gacaataatt gtttatagca caaaccgatt
     1261 gctaccatag aattactaat aatcatacca cctacaggaa tcatttgatt cggaacaaat
     1321 tttaaaattc ctgaaaaaat caagacagat aaagtgatta tagtccctga cccaatagct
     1381 ataaatgaaa taacaaaccc tttattaatc cctttaccac gcttagctgc gttataagaa
     1441 gcattaataa tcatgaatag cattaaaaga gctgtgaaaa taggattttg ataaccaaat
     1501 atatagtcta gcaaaaaccc cactgctaat aattgaatca ctgctcggat agcgctaata
     1561 atcacttctt tttctaggtt taacttttgc cagtaagaaa aaaataaagt aatcaaaact
     1621 aatgatgatg ccattagtaa tgacattaaa gaaatagaat ttgcaccgct cattttataa
     1681 cctccattgc ttcaatacta ccattgacaa cttttaagac tttattggca aatcgacgac
     1741 tctgctcctc atcatgcgta atccacaaaa tcgttattcc tttgtcatgt aatgatttaa
     1801 taaccttttc aacaatagct ttattatgat tgtctaaagc cgaagtaact tcatctaaga
     1861 gtaaaacttt aggttcaaac aataattgcc taatcagagc aatacgttgc ttttctcctc
     1921 ctgatagttt tttgacatcc tgtttcaggt aagactggtc catttcaaaa cgttgaaata
     1981 aatcatcaac gcgtctataa tctacttttt cgtgtcgaat gtggtaagga aaactaatat
     2041 tgtcttccac tgtattacca aataaatgtg gcgtttgaaa acagtaagaa atcatttttc
     2101 gactttcaat cggttctaat tggttcaaat cttttccatc aaagaaaata tgacctgcag
     2161 taggactcat taaaccactg gccaatttta aaacagtact cttaccactt ccagatggtc
     2221 ctacaataga aacaaaatca ccttcatcaa ttgcaaagtt gatatcattt aaaaccaact
     2281 tatcatccgt tttaaaatca acgtgtttaa aggttaaata ggacattgtt aagctccttt
     2341 acttcttatt tataatcatt ataattaagt ctaacataaa aatcaaaaaa aagctcttta
     2401 gaataattct aaattaataa gtaaacttat tttacctctt attaaaaatt ttctgataaa
     2461 ataggctttg taagaggcct tataaacaag atagttatca tccaagt
//
LOCUS       DATRTN010000142         2472 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.143, whole genome shotgun sequence.
ACCESSION   DATRTN010000142 DATRTN010000000
VERSION     DATRTN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.143"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            390..887
                     /gene="ybeY"
                     /locus_tag="VBE22_001942"
     CDS             390..887
                     /gene="ybeY"
                     /locus_tag="VBE22_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEO7203364.1"
                     /translation="MYVEMIDETGQVSEDIKKQTLDLLEFAAQKTGKENKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPEVDISFDEEDLAENPELAEMLEDFDSYIGEL
                     FISIDKAKEQAEEYGHSYEREMGFLAVHGFLHINGYDHYTPEEEKEMFSLQEEILTAY
                     GLKRQ"
     gene            868..1266
                     /locus_tag="VBE22_001943"
     CDS             868..1266
                     /locus_tag="VBE22_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEO7203365.1"
                     /translation="MDLNDNNQKKWKNRTLTSSMEFAVTGIFTAFKEERNMRKHLVSA
                     ILVILAGLTFQVSMVEWLFLLLSIFLVITFEIINSAIENVVDLASNYHFSMLAKNAKD
                     MAAGAVLVVSLFAVLVGLIIFIPKILALLL"
     gene            1308..2207
                     /gene="era"
                     /locus_tag="VBE22_001944"
     CDS             1308..2207
                     /gene="era"
                     /locus_tag="VBE22_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEO7203366.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGD
                     DMIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALQGNNVPT
                     LIKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
BASE COUNT          840 a          334 c          493 g          805 t
ORIGIN      
        1 atctatttat ataaagagac attctaaatg taaatttgtc ataatcgtta tagcatttgt
       61 agctttgttt atttaggaat tagttttacg taagttaggc gctctaaagt ttgtattttg
      121 aagtaaatac taattcataa gtcagaattt tattgaaatg gattataagt gtgtaatagt
      181 gattattagc tttctctccc ttttttaata ctcttatggt ataatgatgt gaaagaatag
      241 cttgaaggaa ggaagagaac gcacttttat tttgagaact agttttagat aaaagtgaaa
      301 aacttaggaa agtagatgtg tcgattattt agaattatgc attacacttt atcttttcta
      361 aacgttttgg gtcgttctta ctatataaga tgtacgttga aatgattgat gaaactggac
      421 aagtttcaga agatatcaaa aagcaaactc ttgatttatt agagtttgca gcacaaaaaa
      481 caggtaaaga gaataaagaa atggctgtaa cgtttgtcac taatgaacgt agccatgaat
      541 tgaatttaga gtatcgagat accgatcgtc cgactgatgt tatcagttta gaatataaac
      601 ctgaggttga tatttctttt gatgaagaag atctggctga aaatcctgaa cttgctgaga
      661 tgctagaaga ttttgattct tatattggtg aactttttat ttctattgat aaagctaaag
      721 aacaagcaga agagtacggt cactcttatg aacgtgaaat gggcttttta gctgtacatg
      781 gttttttgca tattaacggt tatgatcatt atacacccga agaagaaaaa gagatgttca
      841 gcttacagga agaaatttta actgcttatg gacttaaacg acaataatca aaaaaaatgg
      901 aaaaatcgca ccttaacatc aagcatggaa tttgctgtta cagggatttt tacagctttt
      961 aaagaagaaa gaaatatgcg taagcatttg gtttctgcca ttttagtaat acttgctggt
     1021 cttacttttc aagtatctat ggtggagtgg ttatttcttt tattgagtat ttttttggta
     1081 attacttttg aaattatcaa ttcagctatt gaaaatgtag ttgatttggc aagtaattac
     1141 catttctcga tgctggctaa aaatgctaaa gatatggcag caggagcggt tttagttgtt
     1201 tccttatttg ccgtattggt gggattgatt atttttatac ccaagatact tgctttactt
     1261 ttatgatgag aagtattacg aataaattga atggagaaag aaaagaaatg acatttaagt
     1321 caggatttgt agcaatttta ggtcgtccaa atgttggaaa atcaaccttc ctaaaccatg
     1381 ttatgggaca aaaaattgct atcatgagtg ataaagctca aacaacacgt aataaaatta
     1441 tggggattta tacaacagaa actgagcaaa ttgttttcat tgatacccca ggaattcata
     1501 aaccaaagac cgcccttggt gattttatgg ttgaatctgc ttatagtact ttacgtgaag
     1561 ttgaaacagt tcttttcatg gttcctgccg atgaaaagcg aggcaaaggc gatgatatga
     1621 ttattgaacg tcttaaggct gccaagattc ctgttatttt agtaatcaac aaaattgata
     1681 aggttcatcc agaccaactt ttggaacaga ttgatgactt ccgtagtcaa atggatttta
     1741 aagaagtagt gccaatttct gcccttcaag gaaataatgt gccaacccta atcaagttat
     1801 tgactgataa tcttgaagaa ggtttccaat atttcccaga agatcagatt actgatcatc
     1861 cggaacgatt tttagtatca gaaatggttc gtgagaaggt acttcattta acgcaacaag
     1921 aggttcctca ctcagtagct gttgttgtcg aatccatgaa acgtgatgaa gaaactgata
     1981 aagtgcatat tcgtgcaact atcatggttg aacgtgatag tcaaaaaggc attatcattg
     2041 gaaaacaagg tgctatgctc aagaaaatag gaaaaatggc gcgccgtgat attgaactta
     2101 tgttaggtga taaagtttac ctagagactt gggtaaaagt caagaaaaac tggcgtgata
     2161 aaaagcttga tttagctgat tttggctata atgaaaaaga gtattaatga gtttattttg
     2221 agatccgcaa gttagtgggg aagagttaga taagttcacc gtgagacttg attcgcacag
     2281 aaaaagcaat tcacgaggat aaaaccaact agtcataatt ttgagattat attcacaaat
     2341 taattaaagg gagccaaaca tgcttgttgg ccccttctta aataaaggag agcacatgac
     2401 tcaagattat atttgctacc tataaagtag aggaggtgag gatatgcata atatattaag
     2461 atttttaggc at
//
LOCUS       DATRTN010000143         2370 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.144, whole genome shotgun sequence.
ACCESSION   DATRTN010000143 DATRTN010000000
VERSION     DATRTN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.144"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            39..794
                     /locus_tag="VBE22_001945"
     CDS             39..794
                     /locus_tag="VBE22_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO7203367.1"
                     /translation="MSLISYKNVNKYYGDYHALRQINLEIEPGQVVVLLGPSGSGKST
                     LIRTMNALESIDDGSLVVNGHELANTSSKELVNLRKEVGMVFQHFNLYPHKTVLENIT
                     LAPIKVLKQSKKEAMEIAEKYLKFVNMWERKDSYPSMLSGGQKQRIAIARGLAMHPKL
                     LLFDEPTSALDPETIGDVLSVMQKLANDGMNMVVVTHEMGFAREVADRIIFMADGEIL
                     VDTTDVQDFFDNPTEPRAKQFLSNIINHTSTDL"
     gene            806..1606
                     /locus_tag="VBE22_001946"
     CDS             806..1606
                     /locus_tag="VBE22_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO7203368.1"
                     /translation="MLRRKRLTLYLLSCIFIFLLFYPNSTSANQLSEIKKSGVLKVGV
                     KQDVPNFGYHNAETNQYEGMEIDIAKKIAKSLGVKPVFVPTTAQTREPLMDNGQIDIL
                     IATYTITPERKANYNISKAYYHDEIGFLVRKNSHIKTIKELDGKHIGVAQGATTKVNL
                     EKYAKEHKLKFSYAQLGSFPELAISLYANRIDAFSVDKSILSGYLSPHTTILKEGFNT
                     QEYGIATSKQDKVLIPYVNKLLVSWEKDGSLKHIYQKFKLKPAKVKKE"
     gene            1619..2314
                     /locus_tag="VBE22_001947"
     CDS             1619..2314
                     /locus_tag="VBE22_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7203369.1"
                     /translation="MTLLNISPFAISRWGAFFNHFDLFFKGFLYTLGISFGALLLALI
                     LGILSGGLSTSKSKVGKLISRIYVEVFQNTPLLVQMVFVYYGLAIISNGHVMISAFFT
                     AVLCVGLYHGAYISEVIRSGIEAVPKGQTEAALAQGFTANQTMQLIILPQAVRTILPP
                     MTNQVVNLIKNTSTVAIISGADIMFVAKAWAYDTTNYIPAFAGAAIFYFVICFPLASW
                     ARKQEELNKKTYS"
BASE COUNT          777 a          436 c          422 g          735 t
ORIGIN      
        1 ggtaacctaa caataaactg ttattttagg aggttcttat gtcactcata tcttataaga
       61 atgttaacaa gtattatgga gattatcacg cccttcgtca gattaatttg gaaatagagc
      121 cagggcaagt ggttgttcta ttaggacctt ctggatcagg taaatcaaca cttattagaa
      181 caatgaacgc tctcgaatct attgacgatg gtagtttggt cgtgaatgga catgaattag
      241 caaatacttc atctaaagaa ttggtcaacc tccgtaaaga agttggtatg gtttttcaac
      301 attttaacct ttatcctcac aaaaccgtgt tagaaaatat tacattagct cctattaaag
      361 tattaaaaca atctaaaaaa gaagctatgg aaattgctga aaaatattta aaatttgtca
      421 atatgtggga aagaaaagat tcctacccat ctatgctatc aggtggtcaa aaacagagaa
      481 ttgctattgc tagagggctt gccatgcatc caaaactcct cctctttgat gagcctacct
      541 ctgcccttga tcccgaaacc atcggagacg ttctttcagt gatgcaaaaa ttggcaaatg
      601 acggaatgaa tatggtcgtt gtaactcatg aaatgggatt cgcacgagaa gttgctgatc
      661 gtattatttt tatggctgat ggggaaattt tagtggatac tacggatgtg caagactttt
      721 ttgataatcc cacagaacca cgtgccaaac aatttttaag caacattatc aaccatacca
      781 gtactgacct ttaggaggac gaaatatgct gagacgaaaa agattaacgc tctatctatt
      841 atcctgtatt ttcattttcc tgttatttta tcccaactca acgtcagcta atcaattatc
      901 agaaatcaaa aaatcgggtg tccttaaagt tggagtcaaa caagatgtgc ctaactttgg
      961 ttatcataat gctgagacga atcaatacga aggaatggaa attgatattg ccaaaaaaat
     1021 tgccaaatcc ttaggcgtca aaccagtttt tgtaccgaca actgcacaaa ccagagagcc
     1081 tctgatggac aatggacaaa ttgatattct aatcgcaaca tacactatca cgcccgaaag
     1141 aaaagctaac tataatattt ctaaagctta ttatcacgat gaaattggat ttttggtgcg
     1201 taaaaatagt catatcaaaa ctataaaaga attggatggt aaacacattg gtgtggctca
     1261 aggtgccact acaaaagtaa atttagaaaa atatgctaaa gaacacaaac taaaatttag
     1321 ctatgctcaa ttagggagtt tcccagaatt agcgatatcg ctgtacgcta atcgtattga
     1381 tgctttttca gttgataaat ctatccttag cggctacctt agccctcata caaccatatt
     1441 aaaagaaggg ttcaatacgc aagaatacgg tattgcaacc agtaaacagg acaaagtctt
     1501 gattccctac gttaataagt tactggtatc atgggaaaaa gatggtagtt taaaacacat
     1561 ttatcaaaaa tttaagctta aacccgctaa agtaaaaaaa gaatagggag gattttttat
     1621 gactttatta aacatcagtc cttttgccat ttcaaggtgg ggcgctttct ttaaccattt
     1681 tgacctattc tttaaaggct tcttatatac tctaggtatt tcgtttggtg cactcctact
     1741 tgctctgata ttaggaattc tctctggtgg tttatcaact tctaaaagta aagttggtaa
     1801 gttgatatca cgtatctatg ttgaagtttt tcaaaacaca cctcttttag ttcaaatggt
     1861 ttttgtctac tatggactag caatcattag taatggacat gttatgatta gtgctttttt
     1921 caccgccgtt ttgtgcgttg gcctatacca tggtgcctat atttctgaag ttattcgctc
     1981 aggaattgaa gctgtcccta aaggacaaac agaggctgct ttagctcaag gattcacagc
     2041 taatcaaacc atgcaactta tcattttgcc tcaagctgtc agaacaattt taccaccaat
     2101 gaccaaccaa gttgttaacc taatcaagaa tacctctacc gtagcaatca tttctggagc
     2161 cgatatcatg tttgtagcta aggcctgggc ttatgatacg actaattata tcccagcttt
     2221 tgcaggagct gctatcttct attttgttat ttgtttccca ctagcaagct gggcaagaaa
     2281 acaagaagaa ttaaataaga aaacttattc ttaaggaggt ttgatatgtc atcagtctta
     2341 acactaacaa atatactatt tctccttcaa
//
LOCUS       DATRTN010000144         2214 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.145, whole genome shotgun sequence.
ACCESSION   DATRTN010000144 DATRTN010000000
VERSION     DATRTN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.145"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(1..2205)
                     /locus_tag="VBE22_001948"
                     /pseudo
     CDS             complement(1..2205)
                     /locus_tag="VBE22_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410468.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT          703 a          422 c          281 g          808 t
ORIGIN      
        1 tcactaatcc aaagttcaaa aatcaataag cctttttcaa gacgagggtt cctaaagtct
       61 gattcaaaaa aatgatacag cttatcttct ttttcaatat agaatgccct tgcagttgaa
      121 ttactagttt caattatact gtcaggtgtt attttataag gaaccccatc cggaaattct
      181 gtaacaactt ctgaagtaag tgagttttct aatatcatac ttgtatcaat attagtatct
      241 aaaatttttg ccccaatttt ttggcaccaa tctacccaaa aattaagact ttcaacccac
      301 ctcatccaaa cttttccttt ataagaacaa cctacactta ttttatttcc atctctgtat
      361 ccatgtccaa aaatatttga tttgattgaa cccaatgaag ttgcttcact tattcccttt
      421 cttacatcaa ccccagcaaa catccgaaaa ctgacatttc ctccaggtat ttgttttaaa
      481 ccaagtgttc caatcattag tcgattaata ccgtcaagca ctctgtaaat ctcatctccc
      541 ttgatttgca tagaattagg aaaaatttgc tctaccaaat catttccttt tctactatct
      601 gactcattaa tatgaatcaa acctctatac tcatcaaaaa aagattacaa aaaaattata
      661 taaatattcg acaagatctt tttgataaga ccagttaact gtattctcta tttcttcaat
      721 aaatattaaa attcccttct cttcatttat caagtgagta gtatgttctg cactcagaac
      781 atttttccag gcgttgatat ttacctcttt catatccgtt ttatatattc gagaactaat
      841 tttcatcttt atttgagata agtcaatatt acttgctctt gttttttcaa attgggaaat
      901 aaattcttct atctgaactt ctttttctat tgctctatct gacttaattt gaagcagttt
      961 agtccaatcc gcatcacgtc tatacaattc atcgatatct tcttttaagt cttctgatgc
     1021 tatattagta atcagtttag cactgcctaa cctttcttta ccagaccttg caaaccttcc
     1081 cataaactgt aaagtaattg gtaatgactt atatttgtcg tgaattgcag caactttaag
     1141 tgaaggaata tcaatacctt caccaaacat gtccacgcat acaacaattt gtgattttcc
     1201 tgattttagt agatctaacg aatctctgtt ttgcgacttt tttctatcag aatgtataat
     1261 cactggatta tatattttgt aattcgaata tattttatcg aataactcat cagctctttt
     1321 tttagagtta gctctcgcca aaagaatgtg atttaatccc ttatccaaat cactttgcaa
     1381 aagtttaact gcaacatctg cgattttttc atccgatttt gtaatattat actcatggat
     1441 aggatgaaaa tcaattggtt taaaataatt gttttgcaga gcaagagata aactatattt
     1501 aaaaattatt tttccatcaa ttttcttacc atcatttcga aatggggtcg ctgtaaattg
     1561 taaaatcttt ttacctgaga acttatctct tacttttctc caatcatttg cagcaagatg
     1621 gtgagcttca tcaaatacaa ctaattcaac gttcgaatag attttttcaa acagatcatt
     1681 cggtaacgct ttaatcagcg ctggtgtagt tataatgata tttatattat caatttcatt
     1741 aaataaaata tccttttcac tatctttcac agtagattta tataatagac aattaggaaa
     1801 aattgcctga tcagatacaa ctgaaatttt aggaagaatc cccagatgtt taacctgatc
     1861 aaaaagttgt tctctaagta aattacttgg aacaacaatc aaagttgtag caatacgctc
     1921 tgagactatt gtcgaaaaaa ttgtttcaga ttttcctgtt cctgtcggta aaactattgt
     1981 ggccggttca ttagagacaa cccaatgaga acgtatagca gaaagtgcac catattgagg
     2041 agggcgcaat ccattaccta aagaaccgtt agcaactttc tcagaatacg ctttgcccca
     2101 gctttttaca atctctgatt tggtaatttt ttgattatta aggtctattg aataggctaa
     2161 cttatttgaa ccaatattct taatttcttg tttgaatttt tccatcacaa agcc
//
LOCUS       DATRTN010000145         2169 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.146, whole genome shotgun sequence.
ACCESSION   DATRTN010000145 DATRTN010000000
VERSION     DATRTN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.146"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(34..240)
                     /locus_tag="VBE22_001949"
     CDS             complement(34..240)
                     /locus_tag="VBE22_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEO7203370.1"
                     /translation="MNTEIPNQLLPFLVPLIMLQGALIIISLVKLSKLKMTKHLSKPV
                     WFLVIIFLNIIGPIAFLILEGNNE"
     gene            complement(242..1318)
                     /locus_tag="VBE22_001950"
     CDS             complement(242..1318)
                     /locus_tag="VBE22_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7203371.1"
                     /translation="MLGENIYLQRTQIGMTQENLSDYLHLTKTTISKWENNQAKPDID
                     YLILMANLFDISLDDLVGYQKTLSDDQRNQLIKDLKIKANVLSERDFFQEVKELSKQF
                     PNDFKTLLIMINMVLSNLTNLNDSEMKEWSLSTLNKIISKTVVESDLQTAIMFKTVVL
                     FHNLEYDEVINLYQNSPYKLGEELLLANSFFAKGDVYRAKQVLQVEIYQQILLVCEYL
                     LSLSTFEVEENKGDIFERLEHMIEAFQLNSLHPNTVIKCYYSLACHYSKSDDDKALGY
                     LKQCFKSLKQLLQRFELHGDTFFYTIDDWLETIPTGIAPPTSQLQVIERVEDLFQNSQ
                     FKELSGHKEFQQMIQKLNDLKKDY"
     gene            complement(1567..>2169)
                     /locus_tag="VBE22_001951"
     CDS             complement(1567..>2169)
                     /locus_tag="VBE22_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO7203372.1"
                     /translation="SFFQCVILLVSFLVLTLAVKSQSDMISYLDNITSAFFQSIRNPD
                     LTNLMTIISTVVSPLTTSLIALVILGYQYFLNQRIAVWLFMLFFGTNALALLLKDIIA
                     RHRPMNQLVFDSGYSFPSGHTISTFLLMILVLVVARQRLRRVLSQVVFVIFALVILAS
                     VIFSRLYLENHFLTDILGSLLLGASSYYGLSAIVSLKELQ"
BASE COUNT          771 a          357 c          314 g          727 t
ORIGIN      
        1 aagattttgt aacgttttga aatgagatag ctttcattca ttattccctt ccaaaataag
       61 aaatgcaatt ggcccaatta tattaagaaa tataatgacc aaaaaccaaa ctggctttga
      121 aaggtgttta gtcatcttaa gtttacttag tttgactaga gaaatgataa ttaaagctcc
      181 ttgcaacata atcaacggaa ctaggaatgg taataattga tttggtattt ctgtattcat
      241 attagtaatc cttttttaaa tcgttaagtt tttgaatcat ttgttgaaat tctttatgcc
      301 ctgatagttc tttaaattgt gaattttgaa ataagtcctc aactcgttct attacctgta
      361 attgactcgt cggtggtgca ataccagtag gtattgtctc taaccaatca tcgatggtat
      421 aaaagaaagt atcaccgtgt aattcaaatc tttggagcaa ttgttttaaa gatttaaaac
      481 attgttttaa gtatcctaat gccttatcat catcactttt actataatga caagctaaac
      541 tataataaca cttgatgact gtgttgggat gcaaagagtt taattgaaac gcttctatca
      601 tatgttcaag cctttcaaaa atatcgcctt tgttttcttc aacttcaaaa gttgataaac
      661 tcaatagata ctcgcataca agtagaatct gctgataaat ttcaacctgt aatacttgct
      721 tagctcggta aacatcgcct tttgcaaaaa agctattagc taaaagtaat tcttcaccta
      781 atttataggg actattctgg taaaggttta tcacttcatc atattcaagg ttgtgaaaaa
      841 gaacgaccgt tttgaacatt atcgccgtct ggaggtcaga ctcaactact gttttactaa
      901 taattttatt tagtgtactt agtgaccact ctttcatttc tgaatcatta aggttggtta
      961 ggttgctcaa gaccatatta atcattatca acagagtctt aaaatcgtta gggaactgtt
     1021 ttgataattc cttaacttcc tgaaagaaat ctctctcaga caatacattt gcttttattt
     1081 ttagatcctt aatgagctga tttctttgat catctgacaa tgttttttga taaccgacta
     1141 agtcatccaa gctaatatca aaaagattag ccatcaaaat aagatagtca atatccggtt
     1201 tagcttgatt attttcccac tttgaaattg tagtttttgt taaatgcaga taatcactca
     1261 aattctcttg cgtcatacca atctgagtac gttgtaaata gatattctct ccaagcatcg
     1321 gtcaatctac tttcacgtcc attatagagt ttctaaatca aaaataaaag ttcctatcag
     1381 gaaatataat ataaaaaagt ttccctagaa gaaacttttt cgttgattaa taagtggcaa
     1441 aacagcatta ataataacaa tggttaaata aaaattaact tcactaatat ttgtcattga
     1501 aaaataatga attataaaag agcccttaag aaaggctctt ttataattca ttggtaacta
     1561 ctatttttat tgtaactctt tcaaagatac aatagcagac aaaccataat atgacgatgc
     1621 tcctagtaac aacgacccta aaatatccgt taaaaaatga ttttctaaat agagtcttga
     1681 aaaaataact gatgcaagga tcactaatgc aaatattacg aaaactactt gtgacaatac
     1741 tcttctcaat ctctgtctcg caaccactaa aacaagaatc attaataaaa acgttgatat
     1801 cgtatgtcca ctgggaaaac tataacccga atcaaaaaca agttgattca taggtctatg
     1861 acgtgcgatg atatctttta aaagcaatgc caaagcattg gtaccaaaaa ataacataaa
     1921 tagccacaca gctattcttt ggtttaaaaa atattgatac ccaaggataa caagtgctat
     1981 taatgacgtt gtcaaagggc tgacaactgt tgatattatc gtcattaagt tagttaaatc
     2041 gggatttcgt atgctttgaa aaaaagctga agtgatatta tccaaataac ttatcatatc
     2101 actttgagat ttaactgcta atgttaacac taaaaaagaa acaagtagta taacacactg
     2161 gaaaaagga
//
LOCUS       DATRTN010000146         2149 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.147, whole genome shotgun sequence.
ACCESSION   DATRTN010000146 DATRTN010000000
VERSION     DATRTN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.147"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(1..590)
                     /locus_tag="VBE22_001952"
                     /pseudo
     CDS             complement(1..590)
                     /locus_tag="VBE22_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771531.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            749..>2149
                     /gene="pilA"
                     /locus_tag="VBE22_001953"
     CDS             749..>2149
                     /gene="pilA"
                     /locus_tag="VBE22_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2a pilus adhesin PilA"
                     /protein_id="HEO7203373.1"
                     /translation="MRKYQKFSKILTLSLFCLSQIPLNTNVLGESTVPENGAKGKLVV
                     KKTDDQNKPLSKATFVLKTTAHPESKIEKVTAELTGEATFDNLIPGDYTLSEETAPEG
                     YKKTNQTWQVKVESNGKTTIQNSGDKNSTIGQNQEELDKQYPPTGIYEDTKESYKLEH
                     VKGSVPNGKSEAKAVNPYSSEGEHIREIPEGTLSKRISEVGDLAHNKYKIELTVSGKT
                     IVKPVDKQKPLDVVFVLDNSNSMNNDGPNFQRHNKAKKAAEALGTAVKDILGANSDNR
                     VALVTYGSDIFDGRSVDVVKGFKEDDKYYGLQTKFTIQTENYSHKQLTNNAEEIIKRI
                     PTEAPKAKWGSTTNGLTPEQQKEYYLSKVGETFTMKAFMEADDILSQVNRNSQKIIVH
                     VTDGVPTRSYAINNFKLGASYESQFEQMKKNGYLNKSNFLLTDKPDDIKGNGESYFLF
                     PLDSYQTQIISGNLQKL"
BASE COUNT          833 a          288 c          399 g          629 t
ORIGIN      
        1 tttgtatcgg aggaatatga tttttgataa ttctttctag gtgagcacaa aggagaagaa
       61 aatgttgttc cttaaacgtg tattctccaa tttcacttaa ccaatttgta attatcggtc
      121 ggagaatagt agttaattta tcatgtctat gatagttcca tgaaggaaat gaaattgttt
      181 cagggattaa ttgatttaat cctaaaataa atgttcggga aaaaatgata gcaactttta
      241 ccaactcgtc atgataggag tcagataagg gaagggcatc tttgagtggc tgtaataatt
      301 tttggaattt gggataattt tcgaaaatag ctattacatt atcgatacgc ttctcagtcc
      361 aatttggatt agaaaaagag ttatgggaag taaggtaagc aagaaaaata tagtctatat
      421 cgtctttttt aaattaattt taaaggaatc tataataaca tttttgacac aatattgtaa
      481 tgtatcatag ataaatatat tttgtagttc aatgaaaagg ggtgagtaag ggacaactac
      541 attataatta atacgtttcc atgatagaga caataatgta tcaaataaaa agaaaaatca
      601 ttcaaaaatt gggaaaaaac tttaaaattc cataccttat aataagaaat tattgatatc
      661 ataataagtg atagttttta tattctagga tattctgtat ctgatcttag atttagaaac
      721 gacatttcgg cacaatagga gttgtaaaat gagaaaatac caaaaatttt ctaaaatatt
      781 gacgttaagt cttttttgtt tgtcgcaaat accgcttaat accaatgttt taggggaaag
      841 taccgtaccg gaaaatggtg ctaaaggaaa gttagttgtt aaaaagacag atgaccagaa
      901 caaaccactt tcaaaagcta cctttgtttt aaaaactact gctcatccag aaagtaaaat
      961 agaaaaagta actgctgagc taacaggtga agctactttt gataatctca tacctggaga
     1021 ttatacttta tcagaagaaa cagcgcccga aggttataaa aagactaacc agacttggca
     1081 agttaaggtt gagagtaatg gaaaaactac gatacaaaat agtggtgata aaaattccac
     1141 aattggacaa aatcaggaag aactagataa gcagtatccc cccacaggaa tttatgaaga
     1201 tacaaaggaa tcttataaac ttgagcatgt taaaggttca gttccaaatg gaaagtcaga
     1261 ggcaaaagca gttaacccat attcaagtga aggtgagcat ataagagaaa ttccagaggg
     1321 aacattatct aaacgtattt cagaagtagg tgatttagct cataataaat ataaaattga
     1381 gttaactgtc agtggaaaaa ccatagtaaa accagtggac aaacaaaagc cgttagatgt
     1441 tgtcttcgta ctcgataatt ctaactcaat gaataacgat ggcccaaatt ttcaaaggca
     1501 taataaagcc aagaaagctg ccgaagctct tgggaccgca gtaaaagata ttttaggagc
     1561 aaacagtgat aatagggttg cattagttac ctatggttca gatatttttg atggtaggag
     1621 tgtagatgtc gtaaaaggat ttaaagaaga tgataaatat tatggccttc aaactaagtt
     1681 cacaattcag acagagaatt atagtcataa acaattaaca aataatgctg aagagattat
     1741 aaaaaggatt ccgacagaag ctcctaaagc taagtgggga tctactacca atggattaac
     1801 tccagagcaa caaaaggagt actatcttag taaagtagga gaaacattta ctatgaaagc
     1861 cttcatggag gcagatgata ttttgagtca agtaaatcga aatagtcaaa aaattattgt
     1921 tcatgtaact gatggtgttc ctacgagatc atatgctatt aataatttta aactgggtgc
     1981 atcatatgaa agccaatttg aacaaatgaa aaaaaatgga tatctaaata aaagtaattt
     2041 tctacttact gataagcccg atgatataaa aggaaatggg gagagttact ttttgtttcc
     2101 cttagatagt tatcaaacac agataatctc tggaaactta caaaaactt
//
LOCUS       DATRTN010000147         2116 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.148, whole genome shotgun sequence.
ACCESSION   DATRTN010000147 DATRTN010000000
VERSION     DATRTN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.148"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            161..1729
                     /locus_tag="VBE22_001954"
     CDS             161..1729
                     /locus_tag="VBE22_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO7203374.1"
                     /translation="MNLQKNDKTNSFIHYANGPSLEEINGTIEVPKDLSFFKTLLAYS
                     GPGALVAVGYMDPGNWSTSITGGQNFQYLLISIILMSSLIAMLLQYMSAKLGIVTQMD
                     LAQAIRARTSKQLGIVLWILTELAIMATDIAEVIGGAIALYLLFHIPLAIAVFITVFD
                     VLLLLLLTKIGFRKIEALVVALILVIFLVFAYQVALSHPIWTDIFKGLVPTSEAFSTS
                     HTVNGQTPLSGALGIIGATVMPHNLYLHSSVVQSRKLDHNNKKDIARAIRFSTFDSNI
                     QLTVAFFVNSLLLIMGVAVFKTGSVTDPSFFGLFKALSNSTIMSNSILAHIASSGILS
                     LLFAIALLASGQNSTITGTLTGQIIMEGFIHMKVPIWFRRIITRLISVIPVMICVLVT
                     SGRSTVEEHIAINNLMNNSQVFLAFALPFSMLPLLIFTNSKVEMDDDFKNTWIIKILG
                     WLSVIGLIYLNMKGLPNQIEGFWGDNPTHQQIILAHQIAHIIIISVILTLIWTIVELY
                     KGNKKYSTMQQSAT"
BASE COUNT          700 a          311 c          318 g          787 t
ORIGIN      
        1 aaaaagagta agattctgat tcccttaaac ttttattcct gataagagaa ttctttattt
       61 ttaaaattaa acattgaaaa atttttatca ttgtactaaa attaggtgtg gttaatatat
      121 tatttaacca tactatttca atggaaaggt ttatagttca atgaatctac aaaaaaatga
      181 taaaacaaat agttttatcc attatgcaaa cggtccttcc ctagaagaaa taaatgggac
      241 aattgaagtt cctaaagatt tgagcttttt taaaacttta ctagcttatt ctggaccggg
      301 tgcacttgtt gctgttggtt atatggatcc tggaaattgg tccacttcca taactggcgg
      361 tcaaaatttt caatatttac taatttcaat tatcttaatg tctagtttaa ttgctatgct
      421 acttcaatac atgtcagcta agctaggtat tgttactcag atggatcttg ctcaagctat
      481 ccgagcaagg accagtaaac agttgggaat tgtcttatgg attctcactg agcttgctat
      541 aatggcaaca gatattgctg aagttattgg tggggctatt gcactttatt tactgtttca
      601 tattccactt gcgattgctg ttttcattac agtatttgat gtattactct tattattgtt
      661 gacaaaaata ggatttcgaa aaattgaagc tctagtagtt gctttaatat tggttatttt
      721 cttagttttt gcatatcaag tggctctttc tcatcctata tggacggata tttttaaagg
      781 tttagtacct acatctgaag ctttctcaac ctctcataca gtaaatggac aaacaccact
      841 ttcaggtgcc ttaggcatta ttggggcaac tgttatgcca cataatcttt atctacattc
      901 ttcagttgtt caaagtcgaa aactagatca taataacaaa aaagatatcg ctagagcaat
      961 taggttttca acttttgatt caaatattca attaacagta gccttttttg ttaattcact
     1021 attactcatt atgggcgttg ctgttttcaa aacaggaagt gttactgatc catcattctt
     1081 tggtctattt aaagcactat ctaattctac tattatgagt aattctattt tagctcatat
     1141 agcaagttca ggtattttat cacttttatt tgcaatagct ttattagcat ctggacagaa
     1201 ttcaactatt actggaacat taacaggtca aattattatg gaaggattta ttcatatgaa
     1261 agttccaatt tggttccgtc gtatcattac tcgcctaatt tctgttattc ctgtaatgat
     1321 ttgtgtctta gtaactagtg gtagaagtac tgttgaagaa catatagcta ttaataactt
     1381 aatgaataac tctcaagtct tcttagcatt tgcattacca ttctcaatgt tacctctact
     1441 aatatttact aatagtaaag ttgaaatgga tgatgatttt aaaaatactt ggataattaa
     1501 gattcttggt tggctttcag ttataggatt aatttatctc aatatgaagg gattgccaaa
     1561 tcaaattgaa ggtttttggg gagataaccc aactcatcaa caaattattc ttgcgcatca
     1621 aatagctcat atcatcatta ttagtgtaat actaacttta atatggacta ttgttgaatt
     1681 gtataaagga aataaaaaat acagtacgat gcaacaaagt gcaacttaat ttagtaaact
     1741 aggtagcctc aagtaaagat aaaagtagta gaataattct actactttta ttgatattat
     1801 tttttaagaa gtcaattata acattgaaaa gaactcaaca taaggagttg ctatctattt
     1861 tgataatatt attgatatgt ataaaaaatc ttgttgtaac ctaaaatatt tagagatcat
     1921 tagatacagc cttagtggct ggtgctaact ggactcagaa tttattctta gagataaaaa
     1981 tgtatgtaca tattcccata ttctagataa cttatactaa aaaattttta ttctagaata
     2041 taaaaattac tttaatcttt tttaaaaccc aaaatgacaa atcccttgat tttactagat
     2101 atttttattt agaaat
//
LOCUS       DATRTN010000148         2066 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.149, whole genome shotgun sequence.
ACCESSION   DATRTN010000148 DATRTN010000000
VERSION     DATRTN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.149"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(257..>2066)
                     /gene="ftsH"
                     /locus_tag="VBE22_001955"
     CDS             complement(257..>2066)
                     /gene="ftsH"
                     /locus_tag="VBE22_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEO7203375.1"
                     /translation="SISYQPSGGVVEVSGTYKKAKTIKSANSFTFLGGSVATKVTGFN
                     SVILPNDSSIKSLVSAAEENNTNIQVKHESSSGTWISYIASFLPLVIMIGFFMMMMNQ
                     GGGGGARGAMSFGKNKARSSSKDEVKVRFSDVAGAEEEKQELIEVVDFLKDPKRYKSL
                     GARIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFE
                     DAKKAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNESIIVIAA
                     TNRSDVLDPALLRPGRFDRKVLVGQPDVKGREAILRVHAKNKPLADNVDLKVVAQQTP
                     GFVGADLENVLNEAALVAARRNKKVIDASDIDEAEDRVIAGPSKKDRTISERERAMVA
                     YHEAGHTIVGLILSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKDDMKEQLAGLM
                     GGRVAEEIIFNAQTTGASNDFEQATAMARAMVTEYGMSEKLGPVQYEGNHAMMAGQMS
                     PEKSYSAQTAQLIDDEVRHLLNEARNKAADIINENRDTHKLIAEALLKYETLDAAQIK
                     SIFETGKMPETENDEDKARALSYDEIKEKMQEEDKK"
BASE COUNT          601 a          448 c          317 g          700 t
ORIGIN      
        1 attatactaa atatttctac ttcctgtcaa tactattttt gcattttttc ttttattttt
       61 aaaaagttaa tattatttat agtaactaac cttctatact tgttgaatgg atagcatttt
      121 taccgttgtc atgttcatat ttcatcttct taattcacaa atttaaactt catcttcata
      181 aaaaataccc ttcaaatttt atctaaattt gaagggtatt tgaaatttat aaagttcttt
      241 aaaaatatat gatgacttat tttttatctt cttcttgcat tttttctttg atttcatcgt
      301 atgataacgc tcttgcttta tcttcatcat tttctgtctc aggcatttta cctgtctcaa
      361 aaatcgattt aatctgagca gcatcaagag tctcatattt taagagggct tctgcaatta
      421 atttatgagt atcacggttt tcgttgataa tatcagctgc cttattacgt gcttcattaa
      481 gaaggtgacg aacttcatca tcaataagtt gtgcagtttg agcagaatat gatttttcag
      541 gtgacatttg accagccatc attgcgtggt tgccttcgta ttgaactggt ccaagtttct
      601 cgctcatacc atattcagtt accatagcgc gggccatagc agtggcttgt tcgaagtcat
      661 ttgaggcacc tgttgtctga gcgttgaaaa taatttcttc cgctacacgt cctcccataa
      721 gacctgctaa ttgctctttc atatcatctt ttgaaagaag catttgatct tctttaggta
      781 aagcaatcat atatccacct gcacgaccac gtggtacgat agtaacttta tgaacaactc
      841 gcgcatttga aagaatcaaa ccgacaattg tgtgcccagc ttcatggtaa gcaaccatag
      901 ctctttctct ttcagaaata gtacgatctt ttttagaagg accagcaatt acacggtctt
      961 ctgcttcatc aatatctgaa gcatcaataa cttttttatt tcgtcgcgca gcaactaaag
     1021 cagcttcatt gagaacattc tccaaatcag caccaacaaa tcctggggtt tgttgagcta
     1081 ctacttttaa gtcaacatta tctgctaatg gtttattttt agcatgtact ctcagaattg
     1141 cttcacgacc ttttacatca ggttgtccaa ctagaacttt tctatcaaaa cgtcctggac
     1201 gcagtaatgc gggatctaac acatcactac ggttggtagc agcgataacg ataatacttt
     1261 cgtttccttc aaaaccatcc atttcaatta aaagttggtt gagcgtttgt tcacgttcat
     1321 cgtttccgcc acccatacca gctccacgtc gacgtccaac agcatcaatt tcatcaatga
     1381 aaataatagc acgttcagct tttttagcat cttcaaacaa agagcgtact cgactagctc
     1441 caacaccgac aaacatttca acaaaatctg aaccggaaat actaaagaat ggaacccctg
     1501 cttcaccggc aacagctttt gctaataggg ttttacctgt ccctggaggt ccttctaata
     1561 gaacccctgc tggaattcgg gcacctagag atttatatcg tttaggatct ttaaggaagt
     1621 caacgacttc tattaattct tgcttttctt cctctgctcc agcaacatcc gaaaatcgaa
     1681 ctttaacctc atctttacta gatgatcttg ctttattttt accaaagctc atggcaccac
     1741 gagcgccacc gccaccgcct tggttcatca tcatcatgaa gaaaccaatc atgataacta
     1801 aaggtaaaaa actggcaata taagaaatcc atgtcccact tgaactttca tgtttgacct
     1861 gaatattagt gttattttct tcagcagcgc taacaagaga ttttattgaa gagtcatttg
     1921 gtagtataac agaattaaat cctgtaactt tagtagcaac actaccacct aaaaaagtaa
     1981 aactattagc tgatttaatt gttttggctt tcttatatgt cccactaact tcaacaacac
     2041 caccacttgg ctggtatgag atagac
//
LOCUS       DATRTN010000149         2038 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.150, whole genome shotgun sequence.
ACCESSION   DATRTN010000149 DATRTN010000000
VERSION     DATRTN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.150"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..586
                     /locus_tag="VBE22_001956"
     CDS             <1..586
                     /locus_tag="VBE22_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HEO7203376.1"
                     /translation="QYEKAILTIPKISAVKSQRGRTYGSNIYLDIVLEMNPDLSVFES
                     HAITERVEKLLSDKFSVYDIDIHVEPASIPEDEIFDNVYQKLYKNEKIILAKIPGYET
                     FISPDFYMINEKGNIITSDMLTNATNHSLASNFKYFNVKSISQKTKLVSYELEGKRHT
                     SIWRRNEKWFLIYHQITAKSSPYKTRRYQITSLD"
     gene            745..1137
                     /locus_tag="VBE22_001957"
     CDS             745..1137
                     /locus_tag="VBE22_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO7203377.1"
                     /translation="MKLYVQLMVILTFSFAGEVLSTIFNLPVPGSIIGLILLFLALKY
                     KIIRLRHIDAVGNFLLANMTILFLPPAVGLMEHFQDIKPYLFGIAIIILGALFLNILT
                     IGLVSQWIKKRYEGDYPEIGGKNGNFNE"
     gene            1118..1813
                     /locus_tag="VBE22_001958"
     CDS             1118..1813
                     /locus_tag="VBE22_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEO7203378.1"
                     /translation="MATLTNNPIFGIMLTVWAYYIGIRIFRKYPSPATTPLLLATILL
                     IAFLKLTHISYKDYYNGGSFLTMLITPSTVVLAIPLYRTFHLMKHHIKSISISIILAS
                     VINTVFTAIVAKFFGMKYFLVISLFPKSVTTAMAVGITSKAGGLATITLVVVVITGIL
                     TSVLGPIFLKLLRIEDPVAIGLALGGTGHAIGTGQALKYGQVQGAMAGLAIGITGICY
                     VIVSPLVAGLILK"
     gene            1882..>2038
                     /locus_tag="VBE22_001959"
     CDS             1882..>2038
                     /locus_tag="VBE22_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203379.1"
                     /translation="MIPLKKITVGTIICLSFLGLTACSSSNTQQTSTSKSNVSQHKNI
                     KADHEELR"
BASE COUNT          688 a          349 c          328 g          673 t
ORIGIN      
        1 acaatacgaa aaagctatat taacaattcc taaaatatcc gctgtaaaat ctcaacgtgg
       61 acgtacttac ggtagtaata tttaccttga tattgttctc gaaatgaacc ctgatctctc
      121 agtctttgaa agtcatgcca ttaccgaaag agtggaaaaa ctactcagtg ataaattttc
      181 tgtatatgac atagatattc atgtagaacc cgcttctatt ccagaagacg aaattttcga
      241 taatgtttac caaaaattat acaaaaatga aaaaatcatc ctagcaaaaa ttcccggtta
      301 tgagaccttt attagccctg acttttatat gattaatgaa aagggaaata ttatcacttc
      361 tgacatgctt actaatgcta caaatcattc attggcaagc aactttaaat atttcaatgt
      421 aaaatcaatt agtcaaaaaa caaaacttgt gtcttatgaa ttggaaggta aacgacatac
      481 tagtatttgg cgccgtaatg aaaaatggtt cttaatctac catcaaataa ctgccaagtc
      541 aagtccgtat aaaacgagac gatatcaaat cacttccctt gattaatata ctatttaatc
      601 agttactctt gataaaaata gcttacaaaa ataaattaaa aagattgaga aggatctcgg
      661 tctttttaat ttgtttttat gtaaacgttt catagccttg aaaattactt tcagagtagt
      721 agactataat ggaaggagtt tttcatgaaa ttatacgttc aattaatggt tattttaacc
      781 ttttcattcg ctggtgaggt tctatctact atattcaact tacctgtacc aggtagtatt
      841 attggattaa tattactatt cctagcgtta aaatacaaaa tcatcaggct caggcatatt
      901 gatgccgtgg ggaattttct attagcaaat atgaccattt tatttttacc accagcagtt
      961 ggactgatgg agcactttca agacataaag ccctatcttt ttgggatagc tatcattatc
     1021 ttaggagcgc tatttctcaa tattctaact attggactgg tatcccaatg gatcaaaaag
     1081 agatatgaag gagattatcc agaaattgga ggtaaaaatg gcaactttaa cgaataaccc
     1141 tatctttggt attatgttaa ccgtatgggc gtattacatt ggcataagga tttttagaaa
     1201 atatccttcc cctgcaacaa ctccacttct tttggcgact attttattaa ttgctttttt
     1261 aaaattaaca catatctcct ataaagatta ctacaatggc ggttcatttc taacgatgtt
     1321 aattacgcct tcaacggttg tattggctat tcctctctat agaacctttc atttaatgaa
     1381 gcatcacatt aaaagtatta gcattagcat catcttagct tctgttatca acacagtttt
     1441 taccgcaata gttgctaagt tttttggtat gaaatatttc ttagtaatct cacttttccc
     1501 aaagtccgta actacagcta tggccgttgg aattacaagt aaagcaggtg gattagctac
     1561 tatcactctg gtcgtagttg ttattacagg catcttaaca agcgttttgg gtcctatttt
     1621 tctaaaatta ttaaggatag aagatcctgt tgctataggg ctcgcgctag gaggaactgg
     1681 gcatgctata ggaactggac aagccttaaa atatggccaa gttcaaggcg ccatggctgg
     1741 actagcaatt ggtatcacag gaatttgcta tgttattgtc agtcctttag ttgcaggctt
     1801 aatattgaaa tgaggataag gaaagtaaac ccaaaataat tatgttataa tattaaaaaa
     1861 tagaatttag gataaataat tatgataccg ttaaaaaaaa taactgttgg aacgattatc
     1921 tgtctctcat ttttgggttt aacagcatgc tcttcttcaa atactcagca aacctctact
     1981 tcaaaatcaa atgtatcaca acataaaaat atcaaagctg accacgaaga attacgtt
//
LOCUS       DATRTN010000150         2032 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.151, whole genome shotgun sequence.
ACCESSION   DATRTN010000150 DATRTN010000000
VERSION     DATRTN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.151"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(56..667)
                     /locus_tag="VBE22_001960"
     CDS             complement(56..667)
                     /locus_tag="VBE22_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO7203380.1"
                     /translation="MNMKLSKRYRFWQKVIKALGVLALIATLVLVVYLYKLGILNDSN
                     ELKDLVHKYEFWGPMIFIVAQIVQIVFPVIPGGVTTVAGFLIFGPTLGFIYNYIGIII
                     GSVILFWLVKFYGRKFVLLFMDQKTFDKYESKLETSGYEKFFIFCMASPISPADIMVM
                     ITGLSNMSIKRFVTIIMITKPISIIGYSYLWIYGGDILKNFLN"
     gene            760..1109
                     /gene="ssrA"
                     /locus_tag="VBE22_001961"
     tmRNA           760..1109
                     /gene="ssrA"
                     /locus_tag="VBE22_001961"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1434..2015
                     /locus_tag="VBE22_001962"
     CDS             1434..2015
                     /locus_tag="VBE22_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEO7203381.1"
                     /translation="MRTDMSTGASYPKYRFSVGKYTYGYQQFFYEGVNLKEIGAFCSI
                     AQNVTITGMNHPTDHITTNPFIYYKSRGFINEDRADLIDEKKNGKVIIGNDVWIGTNV
                     TILPSVTIGNGAIIGAGSVITKDIPDYAVVAGTPAKIIKYRFSEEEITLLNASQWWNW
                     SDEAIKEHISEFSDKKEFFNTLKSISENKNHKL"
BASE COUNT          720 a          342 c          326 g          644 t
ORIGIN      
        1 tacaaactta atatacaata aaaagattgg agataatctc caaccttttt tatttttaat
       61 tcaaaaaatt tttcaaaata tcaccaccat aaatccacaa atagctataa ccgatgatag
      121 aaataggttt cgttatcata atgattgtca caaagcgctt aatagacata ttagatagac
      181 cggtaatcat caccatgatg tccgctggtg aaatgggaga tgccatacaa aagataaaaa
      241 acttttcata tccggatgtt tctagcttag actcatattt atcaaatgtc ttctgatcca
      301 taaatagcaa aacgaatttt cgtccataaa atttgactaa ccaaaaaagg atgacacttc
      361 cgatgataat cccaatatag ttataaataa agcctaaggt aggaccaaaa attaagaaac
      421 ctgctactgt tgtaacacct ccaggaatta ctggaaaaac aatctgtaca atttgagcca
      481 caataaaaat cattggaccc caaaattcat acttgtgtac taggtctttt aattcattgc
      541 tatcattcaa aataccaagt ttgtacaaat aaacaactaa gacaagggtc gctattaacg
      601 ctaagacacc caaagcctta atgaccttct gccaaaagcg gtaacgtttt gataatttca
      661 tattcatgct ttaatcataa cacaactgtt aaatttttgc tattttaaat agtatttgtt
      721 atactatatt ggtaagtttt tatgctttat tctacttttg gggtcgttac ggattcgaca
      781 ggcattatga ggtatatttt gcgactcatc ggcagatgta aaatgccagt taaatataac
      841 tgcaaaaaat acaaattctt acgcattagc tgcctaaaaa acagcctgcg tgatcttcac
      901 aagattgttt gcgttttgct agaaggtctt atttatcagc aaactacgtt tggctactgt
      961 ctagttagtt aaaaagagat ttatagactc gctatgtgag ggcttgagtt atgtgtcatc
     1021 acctagttaa atcaatacat aacctatagt tgtagacaaa tatattagca gatgtttgga
     1081 cgtgggttcg actcccaccg gctccatatc attttatcat aacttactat acatacttaa
     1141 aaccttgtta ttacaaggtt tttattttgt ttctttgttc atacattgcc cctatcttag
     1201 gaaaaaaata ctgggaacag actctaacat tgaattaggc cacctcatta gctcactctt
     1261 tgtcaatatc cgagaataag ttctttatca ttctaattta tagaatttat taaaaaaggt
     1321 aaggttttta gaggaaaaaa tgttataatc tatttagtct acgattggag gctagttatg
     1381 gctagtattt taaaaaacat ttagagaaac gtttagagct taaaaatgat cctatgagga
     1441 cagatatgag tactggtgcc agttacccta aatatagatt ctcggtagga aagtacacat
     1501 atggatatca acaattcttt tacgaaggcg ttaacttaaa agaaattgga gcattttgct
     1561 caatagccca gaacgttact attactggta tgaatcatcc aaccgatcac attaccacta
     1621 acccttttat ttactataag tcaaggggat ttataaatga ggatagggcg gacttaatag
     1681 atgaaaagaa aaatggaaaa gttattattg gcaacgatgt ctggatagga actaatgtca
     1741 ctatacttcc atctgttaca ataggtaatg gagcaattat tggagcaggt tcagttatta
     1801 ccaaagacat tcctgactat gctgttgtcg ctggaacacc tgctaaaatt atcaaatatc
     1861 gcttttcaga ggaagaaata acattattaa atgcatctca gtggtggaat tggtcagatg
     1921 aagctattaa ggaacatatt agcgaatttt cagataagaa ggaattcttc aatactctaa
     1981 aaagtatttc cgaaaacaaa aatcataaac tctagaataa cttcatcaaa ta
//
LOCUS       DATRTN010000151         2002 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.152, whole genome shotgun sequence.
ACCESSION   DATRTN010000151 DATRTN010000000
VERSION     DATRTN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.152"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..999)
                     /locus_tag="VBE22_001963"
     CDS             complement(<1..999)
                     /locus_tag="VBE22_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7203382.1"
                     /translation="MRIIAITEKVIKELFRDKRTLAMMFLAPILIMFLMNVMFSANSN
                     TKVKIGTINVNTKVVSNLDNIKHIQVRSFKFNSSAKKALKSNKIDALISEDNKSYTVF
                     YANTDSSKTTLTRQAFKTAVNTMNSKELISQVKILANKNPKLAQSLQTRSKYIKEKYN
                     YGNKNTGFFAKMIPILMGFMVFFFVFLISGMALLKERTSGTLDRLLATPVKRSDIVFG
                     YMLSYGILAIIQTVVIVLSTIWLLDIQVVGSIFSVIIVNFILALVALSLGILMSTLAK
                     SEFQMMQFIPLIIMPQLFFSGIIPLENMASWAQTVGKILPLSYSGDALTKIIMYGQG"
     gene            complement(1002..1718)
                     /locus_tag="VBE22_001964"
     CDS             complement(1002..1718)
                     /locus_tag="VBE22_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7203383.1"
                     /translation="MKKVIDLKKLQKAYASETVLNNINLEVFKGEIIGLIGPSGAGKS
                     TLIKTMLGMEKADKGTALVLDTQMPDRNILNQIGYMAQSDALYESLTALENLLFFGKM
                     KGIQKTELKQQITHISKVVDLENQLDKFVSGYSGGMKRRLSLAIALLGNPTVLILDEP
                     TVGIDPSLRRKIWQELINIKDEGRSIFITTHVMDEAELTSKVALLLRGNIIAFDTPLH
                     LKKQFNVSTIEEVFLKAEGE"
BASE COUNT          660 a          315 c          315 g          712 t
ORIGIN      
        1 tccttgaccg tacataatta tttttgtaag tgcatcacca gagtaggata gcggtagaat
       61 tttaccgact gtctgagccc aacttgccat attttctaga gggattattc ctgaaaagaa
      121 taattgaggc attataatta agggaataaa ttgcatcatt tgaaattctg attttgctag
      181 agtagacatt aagattccta atgataaggc aaccagagcc aaaataaaat tgacaatgat
      241 gacactaaaa atactaccta caacttgaat atctagtagc caaatagttg ataaaacgat
      301 aactaccgtt tgaataattg ctagaatgcc gtatgataac atatatccaa atacaatatc
      361 actacgttta acaggagttg caaggagtcg atcaagtgta ccagaagtac gctcttttaa
      421 taaagccatt ccagaaatta aaaaaacaaa gaagaagacc ataaatccca ttagtattgg
      481 tatcattttt gcaaaaaagc ctgtattttt atttccgtaa ttatattttt ctttgatata
      541 tttggagcga gtttgtaagg attgtgctag tttcggattc ttattagcta aaattttaac
      601 ttgcgaaatc agttccttac tgttcattgt attaacagcg gttttaaaag cttgtcttgt
      661 taaagtcgtc tttgaagaat ctgtattcgc atagaagaca gtataggatt tattgtcctc
      721 cgaaataaga gcatcaattt tatttgattt gagtgctttt ttagcagatg agttaaattt
      781 aaatgatctc acttgaatat gcttaatatt atctaaattt gaaacgacct tcgtgttaac
      841 gttaatagtt ccaatcttaa cttttgtatt actattcgca gaaaacataa cattcatcaa
      901 aaacataatt aaaataggtg ctaaaaacat catagcaagt gttcttttat cacgaaacag
      961 ttcttttata accttttcag ttattgcaat aattctcatg attattctcc ttcagctttt
     1021 aagaaaactt cctcaatagt actcacatta aattgttttt ttaaatgtaa tggagtatca
     1081 aaggcaataa tgtttccacg taatagtagt gcaaccttac ttgttaattc tgcttcatcc
     1141 ataacgtggg ttgtaataaa gatagaacgt ccttcatcct taatattaat tagctcttgc
     1201 cagatttttc tcctcaagga tggatcaatt ccaacggtag gttcatctag gattaaaact
     1261 gtggggtttc caagtagggc gatggctaga gaaagccgtc ttttcatacc tcctgagtaa
     1321 cctgagacaa atttatcaag ttggttttct agatctacta ctttagaaat atgagttatc
     1381 tgctgtttta attcagtttt ttgaatacct ttcatttttc caaagaataa taaattttct
     1441 aaggcagtta aagattcgta taaggcatca gattgagcca tatagccaat ttgatttaaa
     1501 atattacgat ctggcatttg agtatcaaga acaagagctg ttcccttatc tgctttttcc
     1561 atgccaagca tagttttaat caaggtagat ttccctgctc cagagggtcc tattaatcca
     1621 attatttcgc ctttaaacac ctccaaatta atattattta aaacagtttc tgaggcatat
     1681 gctttttgta gtttttttaa atcgatgact tttttcatga taactttttg ctcctctatc
     1741 agtgagtgag tgtttactca cctatttgat atcatatatt atagtgtgta aagacaggct
     1801 tgtcaataaa agagttgata ggaagttaat atactataat agtcaataaa atggcttatt
     1861 tgagataact aattttttat cttaaaaatc agttgcatgg agagcattcg tgaaaatggg
     1921 cacagaataa aacgactaag aatatccttg tcgttttatt ttttatatag ttatacctct
     1981 tttaaaattc aatataataa tg
//
LOCUS       DATRTN010000152         1935 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.153, whole genome shotgun sequence.
ACCESSION   DATRTN010000152 DATRTN010000000
VERSION     DATRTN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.153"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            179..1773
                     /locus_tag="VBE22_001965"
                     /pseudo
     CDS             179..1773
                     /locus_tag="VBE22_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
     gene            complement(1806..>1935)
                     /locus_tag="VBE22_001966"
     CDS             complement(1806..>1935)
                     /locus_tag="VBE22_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEO7203384.1"
                     /translation="FTAKSIARYDKISKSQVFAEYYLIGSLFSIISVLITHWLLLV"
BASE COUNT          686 a          303 c          340 g          606 t
ORIGIN      
        1 tatctataaa tagaagtata tcataccttg ttataacttt caagaaaatt atagtttttc
       61 ggaaagttta aaagttaaaa acaggaattc attcggtaag tacatcaaat atccattctt
      121 tatgatgatc agtattactt attatcgttt aaatagaagg tttaggagga ctggtattat
      181 gtctagtttt aatagaaaaa aacttaagtt tttgggtata tctttggcga ctctaacagc
      241 caccacagtt accctggttg cctgtggtaa tgagtcaaag aactctggag ataataaagt
      301 tattaattgg tatattccta ctgaaatcag tacattggac atttcaaaga atacagatgc
      361 ttattcaaac ttagctatag gtaattcagg cagcaattta cttagaattg ataaagaggg
      421 caaaccaaaa cccgatttag ctaaaaaagt atccgtttct tctgatggtc ttacttatac
      481 tgctacactt cgtgataatt taaaatggtc agatggaagc aaactaagcg ctgaagattt
      541 ttttacacat ggcggagaat agttgatcct aaaactgcat ctgaatatgc ttacttggcg
      601 acagaatcgc accttttaaa tgctgataag attaattctg gagatattaa agatttaaat
      661 aaacttggtg taacagctaa aggcaatcaa gtgaccttta aattgacaag tccatgccct
      721 caatttaaat attatttagc cttctctaat tttatgcctc aaaaacaatc gtatgttgaa
      781 aaagttggta aagattatgg tacaacatca aaaaatcaaa tttactcagg tccttacctt
      841 gttaaggatt ggaatggttc aaatggtaaa tttaaattag tcaaaaataa atattattgg
      901 gattctaaac atgttaaaac aaatagtgtc atagttcaaa ctatcaaaaa accagatact
      961 gcagttcaaa tgtacaagca aggtcagatt gattttgctg aaatatcagg aacttctgct
     1021 atttataacc aaactggttc ggttaaagca ttgacaaatc agaaaattag gcaagctttg
     1081 aatttagcga ctgatcgtaa aggtgttgtt aaagcggcag ttgatacagg ttcaactcct
     1141 gctgaatctc tggtccctaa aaaattagcc aaattaccta atggtgagga tctttccaag
     1201 tatactgccc caggttatac gtataataca agtaaagctc aaaaactttt taaagaaggt
     1261 cttgctgagg ttggtcagtc atctctcaag ttaacaatca cagctgattc ggactctcca
     1321 gcagctaaaa atgctgttga ctatgtgaaa tctacatggg agagtgcttt gcctggtcta
     1381 actgttgaag aaaaatttgt tactttcaag caacgccttg aagatgcaaa aaatgaaaat
     1441 tttgatgtcg tgctcttctc atggggagga gattatccag aaggatcaac tttctatggg
     1501 ttattcacga ctaattctgc gtataattat ggtaaatttt cttctaaaga gtatgacaat
     1561 gcctatcaaa aggcaattac tactgacgcc ttgaaaccag gagatgccgc taatgattac
     1621 aaaactgctg aaaaagcttt atttgatcaa tcatattata acccagttta ttacctaggg
     1681 aaaaaaggac tccaaaattc taaattaaag gggttggtaa gaaattcaac tggtttgaat
     1741 gttgatttta cttatgctta taagacagaa taaaagaaaa gcgttaggat atttctagcg
     1801 cttttctata ccaaaagtaa ccaatgagtt atcaaaacac ttataattga gaacaatgat
     1861 ccaattaaat aatattcggc aaaaacctga cttttagaaa ttttatcgta acgtgcaatg
     1921 gattttgcag taaaa
//
LOCUS       DATRTN010000153         1911 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.154, whole genome shotgun sequence.
ACCESSION   DATRTN010000153 DATRTN010000000
VERSION     DATRTN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.154"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..523
                     /locus_tag="VBE22_001967"
     CDS             <1..523
                     /locus_tag="VBE22_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203385.1"
                     /translation="KDKYIQFDFIFPKAYPNLLRKDNIDTPNVTISLENLGTRELYNL
                     YIGNFEATYFDDGGYYYQLNPILYSNHKVQLNLMFYEKNGYLKEFNQEDKFHLLISPI
                     KFSIYFQDCLNNWYEQSFKLRIFHQVQADRSDEENSLTVSIERVEIYSAPKEISPDSL
                     PWITNPSKLCQFG"
     gene            1007..1633
                     /locus_tag="VBE22_001968"
     CDS             1007..1633
                     /locus_tag="VBE22_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEO7203386.1"
                     /translation="MRYFMIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIY
                     LGQFLGSVSLIFLSLLFAFVLNYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKD
                     GLRKDNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVTLLTFLVMIYLLVFS
                     AQKLAQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            1645..>1911
                     /gene="cadX"
                     /locus_tag="VBE22_001969"
     CDS             1645..>1911
                     /gene="cadX"
                     /locus_tag="VBE22_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEO7203387.1"
                     /translation="MKKDSICQVDVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDTR"
BASE COUNT          633 a          315 c          294 g          669 t
ORIGIN      
        1 caaggataag tatattcaat ttgactttat attccccaaa gcatatccaa accttttaag
       61 aaaggataat attgacactc caaatgtcac catctctctg gaaaatcttg gaactagaga
      121 attgtataat ttatatatag gaaattttga agctacttat tttgatgatg gtggctatta
      181 ttatcaactt aatccaattt tatattcaaa ccataaggta caacttaatc ttatgttcta
      241 tgaaaaaaat ggttatctta aagaatttaa tcaggaagat aaatttcatt tgctcatttc
      301 tcctatcaaa ttttcaatct attttcagga ttgcttgaat aattggtacg aacaatcgtt
      361 taaattacgt attttccacc aagttcaagc agacaggtca gatgaagaaa attcactaac
      421 agttagtatt gaaagagtag agatatattc tgcccctaaa gaaatttctc ccgattcact
      481 tccgtggata actaatccaa gtaagttatg ccagtttggg tgaatcacta ataaagcgac
      541 cacactctta tccaatttat tagcagtgaa ctaaattttg tctgattttt gtctgattaa
      601 aactgatgca agggcaaaat cactctattt catcaataat tgaaagattt aaaagcccgt
      661 aaaatcaacg tttctgaacg taacaaatat tgctaaatca cacattacgc ttactgaagt
      721 taagtaaggg tttcagtaca cgtcaataga cgtcaaaata ttgattttaa gcgatttttg
      781 attttttgaa actcattata tcgcactaca aatcaataaa aactctacct tttactctac
      841 cttttttaga taaatatatc atctggatat tgaagttaag ccctgctatc atttcgatgg
      901 cagggctttt taatatttgt acactaactt cttatctata ttttggtgga ttttaagttg
      961 acatctacaa agtttaatgt tagaatacat tcaaaaatat atttgaatga ggtattttat
     1021 gattcaaaat gttgttactt caataatcct gtattctggg acagccgtag acttacttat
     1081 tatcctaatg ttattttttg ccaaaagaaa aagcagaaaa gacatcatta acatctattt
     1141 aggacaattt ctaggctctg ttagtctaat attcctaagt ttgctttttg catttgtctt
     1201 aaattatatt cctagtaaag agattttagg tttactcggt ttgattccaa ttttcctagg
     1261 cctaaaagtt ttgcttttag gagattctga tggagaagct attgcaaaag atggtttgcg
     1321 aaaagacaat aaaaacctga tttttctagt cgctatgatt acttttgcaa gttgtggcgc
     1381 tgacaatatt ggtgtctttg tcccatattt taccacctta aatttagcga atttgatagt
     1441 gactttactt acttttctag tcatgattta tctcttggtt ttttctgcac aaaaattagc
     1501 acaagtccct tctgttggag aaactttgga aaaatatagc agatggttta ttgccgttgt
     1561 ctatttagga ttggggatgt atatcctgat tgaaaacaac agctttgaca tgctatgggc
     1621 tgtgttaggc taggagaaaa aattatgaaa aaagatagta tctgtcaagt ggatgttata
     1681 aatcaacaaa atgttacaac cgcaacgaac taccttgaaa aggaaaaagt ccaaaaatca
     1741 cttcgcattt tatcaaaatt taccgataat aaacagataa atatcatctt ttatctcctt
     1801 gccgtcgaag aactctgtgt ctgcgatata gcctgtttat taaatctcag tatggcatct
     1861 gcctcccacc atcttcgtaa actagccaat caaaacatct tggacactag a
//
LOCUS       DATRTN010000154         1831 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.155, whole genome shotgun sequence.
ACCESSION   DATRTN010000154 DATRTN010000000
VERSION     DATRTN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.155"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..1073
                     /locus_tag="VBE22_001970"
     CDS             <1..1073
                     /locus_tag="VBE22_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO7203388.1"
                     /translation="FPDQWQDKLTQRQFDDLTDIQNKLFQAITDGDNILGISPTGTGK
                     TLAYLFPTLLKLQPKKSQQLLILAPNSELAGQIFDVTKEWAEPLGLTAQLFLSGSSQK
                     RQIERLKKGPEILIGTAGRVFELVKLKKIKMMNINTIVLDEFDELLGDSQYHFVDNII
                     NRVPRDQQMIYISATNKLDNSKLADNTITIDLSDQKLDTIKHYYITVDKRERTDLLRK
                     FSNIPDFRGLVFFNSLSDLGACEERLQFNRASAVSLASDINIKFRKVILEKFKNHDIS
                     LLLGTDLVARGIDIDNLEYVINFDIARDKETYTHRSGRTGRMGKEGCVITFVTHKEEL
                     KQLKKYATVAELVLHNQKLHLK"
     gene            complement(1104..>1831)
                     /locus_tag="VBE22_001971"
     CDS             complement(1104..>1831)
                     /locus_tag="VBE22_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO7203389.1"
                     /translation="NKEFLKTGRVTDGLDVKDGKLIINDLKLPLDKLYNVIDESYLKS
                     SDLDLVSNLKAKAPRVALTFDDGPNEKTTPKALEILKRYNAKATFFVMGQSAVGHTDI
                     LKRMHAEGHEIGNHTWDHPNLTKLPAEKIKEEIHKTNDLIMKATGQKPVYLRPPYGAT
                     NATVKTVTGLKEMLWSVDTEDWKNHNTQAMMTNIKKQLRPGGVILMHDIHQTSIDALP
                     TIMDYLITQGYYFVTVGELYSTP"
BASE COUNT          597 a          342 c          294 g          598 t
ORIGIN      
        1 aatttccaga ccaatggcag gataaattaa cccagcgtca atttgatgat ctaacagata
       61 ttcaaaataa actttttcaa gctattactg atggtgataa tatccttgga attagcccta
      121 caggtactgg taaaactcta gcataccttt ttccaacgct tcttaagttg caacctaaaa
      181 agagccaaca actcttaatt ttagctccaa attcagaatt agctggacaa atcttcgacg
      241 ttacaaaaga atgggctgaa ccattaggac tgacagcaca actattcctt tcagggtcta
      301 gtcaaaaacg ccaaattgag cgactaaaaa aaggaccaga aatccttata ggaactgctg
      361 gacgcgtatt tgaacttgtg aaactaaaaa aaataaaaat gatgaacatt aatactatcg
      421 tccttgatga gttcgatgaa ttactcggcg actcacaata tcattttgtg gacaatatta
      481 ttaatcgcgt tcctcgtgat cagcaaatga tttacattag tgccaccaac aaactggaca
      541 atagtaaact tgccgataac actataacaa tcgatctatc tgatcaaaaa cttgatacta
      601 taaaacatta ttatattaca gttgataaaa gagagcgtac tgacctactt cgtaaatttt
      661 caaatattcc tgattttaga ggtctcgtct tttttaacag cttatcagac ttaggtgcat
      721 gtgaagaacg tttacaattt aatagggcct cggcagtttc tttagcaagc gatattaata
      781 ttaaattccg taaagttatc ttagagaaat ttaaaaacca tgatatttca ttactcctag
      841 gaaccgattt ggtagctcgt ggcattgata tcgacaacct agaatatgtg attaattttg
      901 atatcgcacg cgacaaagaa acatacacac atcgttctgg acgaactggt cgtatgggga
      961 aagagggatg tgttattaca tttgtaacac ataaagaaga gctcaaacaa cttaaaaaat
     1021 acgctaccgt tgcagaactt gtacttcaca atcaaaaatt acatttaaaa taaaaaagaa
     1081 acaagtaact tgtttctttt ttattaaggc gttgaatata attcacctac agtaacaaaa
     1141 taataaccct gggttatcag ataatccata attgtaggca gagcatctat ggatgtctgg
     1201 tgaatatcgt gcatcaaaat cactccccca ggacgtaatt gttttttgat attggtcatc
     1261 attgcttgcg tattatgatt tttccagtct tctgtatcaa ctgaccacaa catttctttc
     1321 aatcctgtca ctgtttttac agtagcattt gttgctccgt aagggggacg taagtacacc
     1381 ggtttttgtc ctgttgcttt catgatcaga tcattagtct tatgaatctc ttccttgatt
     1441 ttctccgctg gtagtttggt taaattagga tgatcccatg tatgattacc aatctcatga
     1501 ccttcagcat gcatcctttt taaaatatct gtgtgcccta cagcactttg tcccattaca
     1561 aagaaagtcg ctttagcatt atatctttta aggatttcta aggctttagg agtcgttttt
     1621 tcatttggtc catcatcaaa ggtaagtgca acacgtggtg ctttagcttt taagttagaa
     1681 actaaatcta gatcacttga ttttaaatat gactcatcga tcacattata taatttatca
     1741 agtggtaatt ttaaatcatt aataattaac ttaccatctt taacatctag cccatctgtt
     1801 actcttccag tttttaaaaa ttctttattg c
//
LOCUS       DATRTN010000155         1816 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.156, whole genome shotgun sequence.
ACCESSION   DATRTN010000155 DATRTN010000000
VERSION     DATRTN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.156"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            175..1467
                     /locus_tag="VBE22_001972"
     CDS             175..1467
                     /locus_tag="VBE22_001972"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEO7203390.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDNKKFKLHLIPSGIFFKEKISVIGNGVVVNPKSLVKELAYLHGEGVTTDNLRISDRA
                     HVILPYHIKLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDREVFAERL
                     KINLAEKNRLFEKMYDSTPLEFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWSEDITACRSLDDLPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWSNI"
     gene            complement(1490..1783)
                     /locus_tag="VBE22_001973"
     CDS             complement(1490..1783)
                     /locus_tag="VBE22_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203391.1"
                     /translation="MILINLIALPICFLVELSAGLLLKLEMHKQHGYYPLERLLMTGN
                     ILTVVSLVLLHTLIHNINSLLFLSITVVLLISGKSLSLISIYLLKRKSKHQKV"
BASE COUNT          574 a          296 c          369 g          577 t
ORIGIN      
        1 acacttctaa taattatttt tttctttttt tccgataata tttgaggaat ttggcaaaaa
       61 aaaacatttt ttgctataat aaggtgtcag acaaactcgc aggtgaaatt cctgccatgt
      121 ttttccagaa aggattgggg agtcccaaaa aggctcactc agtgttaaat aaatatgact
      181 tcagttgttg tagtaggtac ccaatggggt gacgaaggta aaggtaaaat tacagatttt
      241 ttatctgctg acgcagaagt aattgcacgt tatcaaggtg gtgataacgc tgggcatact
      301 atcgttattg ataacaaaaa atttaaactt catttaattc cttcaggaat tttcttcaaa
      361 gaaaaaattt ctgtcattgg taatggtgtt gtcgttaatc cgaaatcatt agttaaagag
      421 ttagcttatc ttcacggaga aggtgtaaca acggataacc ttcgtatttc agaccgtgcg
      481 catgttatct tgccatacca catcaagctt gaccaactac aagaagatgc taaaggtgat
      541 aataaaattg gaacaaccat caaaggaatt gggccagctt acatggacaa ggctgcgcgt
      601 gtcggtatcc gtattgctga ccttcttgat agagaagttt ttgctgagcg tttgaaaatt
      661 aatttagctg agaaaaatcg tttatttgaa aaaatgtatg acagtacacc tcttgaattt
      721 gatgatattt tcgaagaata ttatgaatat ggtcaacaaa ttaagcaata tgtgactgat
      781 acatccgtta tcttaaatga tgctcttgat gccggtaaac gtgttctttt tgaaggagca
      841 caaggagtta tgctcgacat tgatcaagga acatacccat ttgtaacatc ttccaatcca
      901 gtagcaggtg gtgtcacaat tggttcggga gttggaccaa gtaaaattaa taaagtagta
      961 ggtgtatgta aagcttacac tagccgtgtc ggtgatggac cattcccaac agaacttttt
     1021 gatgaggttg gtgaccgtat tcgtgagatt ggtaaagagt atggtacaac gactggtcgt
     1081 cctcgtcgcg ttggatggtt tgattctgtt gttatgcgtc acagccgtcg agtatcaggt
     1141 attactaatc tctctctgaa ttcaattgat gttctttcag ggcttgatac ggtgaaaatt
     1201 tgtgtggctt atgaccttga tgggaaacgt attgactatt atccagcaag ccttgaacag
     1261 ctaaaacgtt gtaaaccaat ctatgaagaa ttaccgggct ggtctgaaga tattacagct
     1321 tgccgtagct tagatgatct tccagaaaat gcacgtaatt acgttcgccg tgttggcgaa
     1381 ttggttggtg ttcgtatttc tactttctca gtagggcctg gtcgtgaaca aacaaacatt
     1441 cttgagtctg tttggtcaaa tatttaatat taaaagtctg actgtacagt cagacttttt
     1501 gatgtttact ttttcttttt aatagataga tactaataag acttaaggac ttcccagaaa
     1561 tcaataaaac tactgtaatt gataaaaaaa gaagtgaatt gatattgtga atcaaagtat
     1621 gtaataatac tagcgataca actgttagaa tattaccagt catcaataag cgttccagag
     1681 gatagtagcc atgttgctta tgcatttcta attttaatag tagaccagct gacaattcta
     1741 ctaggaaaca aataggtaga gctatcaaat taataagaat catagaccaa ccaaatcctt
     1801 gaccataact tatata
//
LOCUS       DATRTN010000156         1809 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.157, whole genome shotgun sequence.
ACCESSION   DATRTN010000156 DATRTN010000000
VERSION     DATRTN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.157"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..673
                     /locus_tag="VBE22_001974"
     CDS             <1..673
                     /locus_tag="VBE22_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7203392.1"
                     /translation="DQDDVWLPEKLSVSLEAAKHKASDVPLLVYTDLKVVNQELNILQ
                     DSMIRAQSHHANTTLLPELTENTVTGGTMMINHALAEKWFTPNDILMHDWFLALLAAS
                     LGEIIYLDLPTQLYRQHDNNVLGARTMDKRFKILREGPKSIFTRYWKLIHDSQKQASL
                     IVDKYGDIMTANDLELIKCFIKIDKQPFMTRLRWLWKYGYSKNQFKHQVVFKWLIATN
                     YYNKR"
     gene            675..1406
                     /locus_tag="VBE22_001975"
     CDS             675..1406
                     /locus_tag="VBE22_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO7203393.1"
                     /translation="MMKKILLVIPAYNEEGSIAKTVQTIVDFKASRSLPFELDYIVIN
                     DGSTDGTPELLDRLGLNHIDLVQNLGIGGCVQTGYLYANRNHYDVAVQFDGDGQHDIR
                     SIEDVVMPILNDEADFVIGSRFVDKKHQNFQSTAMRRLGINLISAAIKLTTGHKVYDT
                     TSGYRAANAALIAYLSCHYPVQYPEPESTARILKKGYRLKEVTANMFEREAGTSSISS
                     LKSIFYMTDVLTSIIIAGFIKEDDK"
     gene            1403..1756
                     /locus_tag="VBE22_001976"
     CDS             1403..1756
                     /locus_tag="VBE22_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEO7203394.1"
                     /translation="MSLALQIEMFILAIVLLYMIIRMVNKNTFSVKRSLPWLLVGLIL
                     VFISVFPGVVSFIAHHLGFALTMNFLLFGAILFLFCLELFDTSSNTKRDEQIKRLIQE
                     VSLLKKEIEDSTLDE"
BASE COUNT          581 a          256 c          353 g          619 t
ORIGIN      
        1 tgatcaagat gatgtatggt tgccagaaaa gttatctgta agtttagagg cagcaaagca
       61 taaagcatct gatgtaccat tacttgttta tactgatttg aaagttgtta atcaagaatt
      121 aaatatttta caagacagca tgattcgggc tcaatcgcac catgccaata cgacattatt
      181 accagaacta actgagaata ctgttactgg tgggactatg atgataaatc atgcattagc
      241 tgagaagtgg tttactccta atgatatctt aatgcatgat tggtttttag cactcttagc
      301 agcaagttta ggagaaatca tttatttgga tttaccaact caactatacc gtcaacatga
      361 caataatgtt ttgggggcga ggacaatgga taagagattt aagatacttc gtgaaggtcc
      421 taaatctata tttacaaggt attggaaact tatccatgac tcacaaaagc aagcttcctt
      481 aattgttgat aagtatggtg atattatgac agctaatgat cttgagctca ttaaatgttt
      541 tataaaaatt gacaaacagc cttttatgac aagattaagg tggttatgga aatatggtta
      601 tagcaaaaat caatttaagc atcaagttgt gtttaaatgg ttgattgcaa caaattatta
      661 caataagaga taagatgatg aaaaaaattt tactagtgat acctgcttat aatgaagaag
      721 gtagtattgc aaagacagtc cagactattg tagattttaa ggcgtctcgc tcactacctt
      781 ttgagctgga ttatattgtt attaacgatg ggtcaaccga tggtactcca gagttattag
      841 atagattggg tttaaatcat attgaccttg ttcaaaatct aggtattggt ggctgtgtgc
      901 agacaggata cttatacgct aatcgtaatc attacgatgt tgccgttcaa tttgatggtg
      961 atggtcaaca tgatattaga agcattgaag atgttgttat gccaatctta aatgacgaag
     1021 cagattttgt tattggttct cgctttgttg ataaaaaaca tcaaaatttt caatcgacag
     1081 caatgaggcg tcttggcata aatttaatat ctgctgctat aaaattaacg acgggacaca
     1141 aagtctatga tacaacaagt ggttataggg cagccaacgc ggcgctaata gcttatttat
     1201 cgtgtcatta tccagtacaa tacccagagc ctgaatcaac tgctcggatt ttgaaaaaag
     1261 gctatcgttt gaaggaagtc acagctaata tgtttgaacg tgaggcaggt acttcaagta
     1321 taagttccct aaaatcgatt ttttatatga cggatgtttt aacatcaatt attattgcag
     1381 ggtttattaa ggaggatgat aaatgagttt agcattgcaa attgaaatgt ttattttagc
     1441 cattgtccta ctttatatga ttataagaat ggttaataaa aacacatttt ctgttaaacg
     1501 ttctttacct tggctattag ttggtttaat tctagtcttt attagtgtat ttcctggggt
     1561 ggtctcattt atcgctcatc accttggatt cgctttaacg atgaatttct tgttatttgg
     1621 tgctatttta tttctgtttt gtttagaatt atttgatact agtagcaata ccaaaagaga
     1681 tgagcaaatt aaacgcctga ttcaagaggt ttctttgttg aaaaaagaga tagaggatag
     1741 cactttagat gagtaaaaaa catattttta tttgtctgtt tttcatgctt tttttagtag
     1801 gttgccaaa
//
LOCUS       DATRTN010000157         1786 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.158, whole genome shotgun sequence.
ACCESSION   DATRTN010000157 DATRTN010000000
VERSION     DATRTN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.158"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..429)
                     /locus_tag="VBE22_001977"
     CDS             complement(<1..429)
                     /locus_tag="VBE22_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO7203395.1"
                     /translation="MIIDFKTVSTKGLETSPVAEALAGLRANEARYFWNKFKVAYETF
                     PVSDYPELMADINHLLKNERNLVFAAKPLEISQLFLENRILWTHVFYEDGLAINILYE
                     LDNPKKRAVGLKLSDGMDIPIELADKFKFARQKSKLAGIIR"
     gene            651..1247
                     /locus_tag="VBE22_001978"
     CDS             651..1247
                     /locus_tag="VBE22_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEO7203396.1"
                     /translation="MTKKIFLFICATLLVGIALYTPTTSIFNHNNVYAATKKAKIKFN
                     KTQKKIVKKAREYAKSGHMSKDSIIEKLKKDSKKYRQEDINFVINNLKVDYKKNALIS
                     AKIYSKTMNLSKQSIFEQLYSESPDKATHSDKFTKEESQYAIDHLKVDFKENALETAK
                     SYQSSSSLSKEEIYKQLTSTLGDKFTNDEAQYAVDHLK"
     gene            1436..1786
                     /locus_tag="VBE22_001979"
                     /pseudo
     CDS             1436..1786
                     /locus_tag="VBE22_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          663 a          322 c          272 g          529 t
ORIGIN      
        1 acgtattata cctgcgagtt ttgacttttg acgagcaaac ttaaacttgt ccgctaattc
       61 aataggaata tccattccat ctgataattt taaacctaca gctctctttt taggattatc
      121 taattcatat aaaatattaa tggctaaacc atcctcatag aaaacatgtg tccataaaat
      181 acgattctct aaaaaaagct gtgaaatctc taaaggcttt gcagcaaaaa caaggttacg
      241 ttcatttttt agaaggtgat taatatctgc catcaattct ggatagtctg atactggaaa
      301 tgtttcgtag gctactttga atttattcca gaaatagcga gcttcatttg cacgcaaacc
      361 agccaaggct tctgccactg gagaagtctc caaaccttta gttgaaacgg ttttaaagtc
      421 gataatcatg ttctttcctt tttgattaaa atcttcgata atttagtata ctcttttttg
      481 aagaaaaagt acattctaat cacttataaa aagtagtaca tttagaacac taccacctca
      541 gtaatttctt gacctttctc tacttaatac ttataattaa tctagactta atgtcaaagc
      601 atcaatcaag gtgtaaacct gattcaccta aaaagctgat ggagctaaac atgaccaaaa
      661 agatattctt atttatctgc gctactcttt tagtaggaat agccttatat acaccaacaa
      721 cttctatttt taatcataac aacgtttatg ctgccaccaa gaaagcaaaa attaaattta
      781 ataaaactca aaaaaaaatt gtcaaaaaag caagagaata tgctaagtct ggccatatgt
      841 ctaaagatag tatcattgaa aaattaaaaa aggattctaa gaaatatcgt caagaagaca
      901 tcaactttgt gattaataac ctcaaggttg attacaaaaa aaatgccttg atttctgcca
      961 aaatttattc aaagaccatg aatttatcta aacaatctat ctttgaacag ctctattcgg
     1021 agtctccaga taaggcaacc cactctgata aatttacaaa agaagaatcc caatatgcta
     1081 tagaccatct caaagtagat ttcaaagaaa atgctcttga gactgctaaa agttatcaaa
     1141 gtagttcttc tttatccaaa gaagaaatct ataaacaatt gacatctacg ctgggcgata
     1201 aattcacaaa tgatgaagcc caatatgctg tagatcattt gaaatgatga cataccaata
     1261 gtctatcctt atgtaataga attgatagct tatgctaata ttccagctgt aaaatagcaa
     1321 gaaccctcct aagttcatta agacttagga gggtttttat caatatgaat aggttacact
     1381 aaactagaca gaaattataa agtgttctac acttaagaaa aactggagaa aaaccatgtc
     1441 tagaaaagta cgtcgtcact tcaccgatga ttttaagcaa caaattgttg acctttacaa
     1501 tgttggtaga aagcgtagca gtctcatcaa ggtatatgag ctaacccctt ccaccttcga
     1561 taagtgggtt agacaagcca aaacaactgg ttcattcaag tctgttgata atctgacaga
     1621 tgaacagcgg gagctaattg aactcagaaa acacaataaa gaactcgaaa tgcaattaga
     1681 tatcctaaag caagcagcgg tgattatggc acaaaaaggg aaataatcac tgctaataag
     1741 gataaataca gcatttcaaa gatgtgtcgc tggctgaata tgccac
//
LOCUS       DATRTN010000158         1752 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.159, whole genome shotgun sequence.
ACCESSION   DATRTN010000158 DATRTN010000000
VERSION     DATRTN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.159"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..965
                     /locus_tag="VBE22_001980"
     CDS             <1..965
                     /locus_tag="VBE22_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO7203397.1"
                     /translation="KTDSYQDAYNRIKNGDSQAMVLNNAYVSLIELSTPDFKSQIKTI
                     YTYKIKKKINRKNTNHKEGVFNIYISGIDTFGSISTVSRSDVNIIMTVNTNTHKVLLT
                     TTPRDAYVKIPDGGGNQYDKLTHAGLYGVETSMKTLENLYDINLDYYARINFSSFLKL
                     IDLLGGVTVYNDQAFTSKHGNFDFPVGQVTLNSEQALGFVRERYSLQGGDNDRGRNQE
                     KVIAAIINKLASSQSVTKLNSITSQLQTSVQTNMTIDNINDLINNQLSTGQRFTVESQ
                     ALTGHGSTGELPSYAMPGAQLYMMSIDQSSLSNAKSKIKNTMEE"
     gene            971..1702
                     /locus_tag="VBE22_001981"
     CDS             971..1702
                     /locus_tag="VBE22_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HEO7203398.1"
                     /translation="MIDIHSHIVFDVDDGPKTLEESLSLIEESYRQGVRIIVSTSHRR
                     KGMFETPEDIIFKNFSIVKHEAEKRFEHLQILYGGELYYTSDMLEKLKLKQIPTLNNT
                     KFALIEFSMQTSWKDIHTALSNVLMLGITPVVAHIERYNALENQKERVKEIINMGCYT
                     QINSSHILKQKLFNDKHKRFKKRARYFLEENLVHFVASDMHNLDVRPPFLAEAYKIIC
                     RDFGKERANQLFIENAQSILKNHYI"
BASE COUNT          653 a          256 c          299 g          544 t
ORIGIN      
        1 aaaaaacaga ttcttaccag gatgcataca atagaattaa aaatggtgat agtcaggcta
       61 tggttttaaa taatgcttat gttagcttaa ttgaacttag cacccctgat tttaaatcgc
      121 agataaaaac gatttatact tacaaaatta agaaaaaaat taatcgtaaa aatactaatc
      181 ataaagaagg ggtatttaat atctatatta gcggtattga tacttttggc tctatatcaa
      241 cagtatcaag atctgatgta aatattatta tgacggttaa taccaatacc cacaaagtat
      301 tgttaacgac aacaccacga gatgcctatg taaaaattcc agatggtggg ggcaatcaat
      361 atgataaatt aacccatgca ggtttgtatg gcgttgagac atcaatgaaa acacttgaaa
      421 acctttacga catcaacctt gattattatg ctagaattaa tttttcatca tttttaaaat
      481 taatagacct cttgggagga gtgacagttt ataacgatca agcttttaca agtaaacatg
      541 gtaattttga cttccctgtt ggtcaagtaa cattgaattc tgagcaggct ttgggctttg
      601 ttagagaacg ttattctcta caaggaggcg ataacgatag aggtagaaat caagaaaaag
      661 tgattgcagc tattataaat aagttagctt ctagtcagtc agtaacaaaa ttaaatagca
      721 ttacctcaca gctccaaacg tccgttcaaa ctaatatgac tattgataat attaatgatt
      781 tgattaacaa tcaattgtca actggacaac gcttcactgt cgagtcacaa gcattaactg
      841 gtcatggttc aacgggtgaa ctcccttcat atgcaatgcc aggagctcaa ctttatatga
      901 tgtcaattga tcaatctagc ttatctaatg caaaatcaaa aattaagaac acaatggagg
      961 aataacaaga atgattgata ttcattctca tatcgttttt gatgtagatg atgggcctaa
     1021 aacgcttgaa gagagtttat ctttaataga agaaagttat cgacaaggtg ttagaataat
     1081 tgtttcgaca tcacatagaa gaaaaggtat gtttgaaaca cctgaagata ttatcttcaa
     1141 aaacttttct atagttaaac acgaagcgga aaaaagattt gaacatcttc aaatcttata
     1201 tggtggagaa ttgtactata caagtgatat gttagaaaaa ctgaagttaa agcaaattcc
     1261 aactttaaac aatacgaaat ttgctttaat tgaattttct atgcaaactt cttggaaaga
     1321 tattcataca gctttgtcaa atgttttaat gcttggtatt acaccagtcg ttgcgcatat
     1381 agagaggtat aacgctttag agaatcaaaa agaacgggtg aaggaaatta ttaatatggg
     1441 gtgttacaca caaataaata gttcccatat tttgaaacaa aaacttttta atgataagca
     1501 taaacgcttt aagaaaagag cccgttattt tttagaggaa aatttagtgc attttgtagc
     1561 gagtgatatg cataaccttg atgttagacc gccattttta gcagaagctt ataagattat
     1621 ctgtagagat ttcggtaaag aacgtgctaa ccaacttttt attgaaaatg ctcaatctat
     1681 attaaaaaac cattacattt aggagatttc atgaataaaa tagctaatac agaagttgaa
     1741 ataaatatat tc
//
LOCUS       DATRTN010000159         1742 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.160, whole genome shotgun sequence.
ACCESSION   DATRTN010000159 DATRTN010000000
VERSION     DATRTN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.160"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            177..1037
                     /locus_tag="VBE22_001982"
     CDS             177..1037
                     /locus_tag="VBE22_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutR family transcriptional regulator"
                     /protein_id="HEO7203399.1"
                     /translation="MFIYGPIFREFRLNRQFSLKQVASNELSVSQLSRFERGESDLSL
                     TKFLGALEAIDLSISEFMYRVNKYQKSDQISLMSQMAQYHYQRDVAGLEKMISVEEGK
                     LKKDSSDIRCRLNIVLFRGMICECDSSRKMSEEDLCFLSDYLFQKDSWEISDYILIGN
                     LYRYYNTRYICQLVKEVINQKEYYRDIYTNRNVVEATLLNVVETLIERRALEEATFFV
                     EKVEALLNNERNAYHRIILLYEKGFLAYAKGDSRGIQSMKQAIFCFQAIGSKHHVENF
                     QEHFNRVTRL"
     gene            1110..1742
                     /locus_tag="VBE22_001983"
                     /pseudo
     CDS             1110..1742
                     /locus_tag="VBE22_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024508.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          571 a          232 c          326 g          613 t
ORIGIN      
        1 agaaaaattc ccagataaat taacatatgt tgatttagaa gaacttgtga tatgatatta
       61 caaataaaag aaaatgacta tatatcagct catatattgg ttaaagttgg taaatataaa
      121 ttattgaaat tacctcataa gtttaccaat gagattacgg ttagaaagga atactaatgt
      181 ttatatatgg ccctattttt agggaatttc gacttaatcg acagttttct cttaaacagg
      241 ttgcttcaaa tgaactttct gtttctcagt tatcacgatt tgagaggggt gaatcagatt
      301 tatcattaac aaaattttta ggggctttag aggcaattga tttatcaatt agcgaattta
      361 tgtatagagt aaataaatat caaaaatctg atcagatatc tttaatgtct cagatggctc
      421 aatatcatta tcaaagagac gtagctgggt tagaaaagat gatttctgtg gaagaaggta
      481 aactaaaaaa ggatagttca gatatacgtt gtcgtttaaa catagttctt tttagaggaa
      541 tgatatgtga atgtgactcc tcgagaaaaa tgtcggaaga agacctttgc tttttgtcgg
      601 attacctctt tcaaaaagat agttgggaaa ttagtgacta tattttgatt ggaaatcttt
      661 accggtatta caacacaagg tatatttgcc aacttgttaa agaggttatc aatcaaaagg
      721 aatattatcg agatatttat acaaaccgca atgttgtcga agcaaccctc ctaaatgtgg
      781 tagagacact aatagaacgg cgcgctctag aagaagcaac attttttgta gagaaagttg
      841 aagcactttt aaataatgag cggaatgctt atcaccgtat tattttactc tatgaaaaag
      901 gatttcttgc ctatgctaaa ggggatagtc gaggaataca aagcatgaag caagctattt
      961 tttgcttcca agcaattggt tctaaacatc atgttgaaaa ttttcaggaa cactttaata
     1021 gggtaacaag attataaaaa atcccattta tgggattttt taatatttct ccttttggtt
     1081 tgctataatg tcgaactaag gagggaatta tgaatattaa tggtataaaa ttactatcta
     1141 gtcgagcagt cagtaaactt ggtgatgtgc tttatgatta tggaaatagt acttggattg
     1201 cttcgatggg tggtctagga caaaagattt taggtattta tcagatagta gagttgttgg
     1261 tttctattgt cctcaaccct tttggaggcg ccttagctga tcgctttcaa cgaagaaaaa
     1321 ttttactaat aacagatgct atctgtgcta tcatgtgttt cttactttct tttattggag
     1381 atgataaagt aatggtgtat ggtctaatag ttgctaatgc tatcttagcg gtttccaatg
     1441 ctttttcttc tccggcttat aaatcttata ttcctgaaat agtagataaa gctgatatca
     1501 ttacctataa tgctaatctg gaaaccattg tgcaaattat tagtgtaagt tcccctgttt
     1561 taggtttcct tatttttaat aattttggta ttaggattac tttaattgtt gatgcgatta
     1621 cttttttgat ctctttcctg tttctttatg ctattaaagt ggaagggtac agctttcaaa
     1681 acaagaaaag gtagcgataa aaaatatttt agctgatatt gcagatggat ttacttacat
     1741 ca
//
LOCUS       DATRTN010000160         1733 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.161, whole genome shotgun sequence.
ACCESSION   DATRTN010000160 DATRTN010000000
VERSION     DATRTN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.161"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            252..785
                     /locus_tag="VBE22_001984"
     CDS             252..785
                     /locus_tag="VBE22_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203400.1"
                     /translation="MGNDNVTIKLEHRVKTSADLSDYVYKYERAYTDALNNGDDIKEA
                     SESTKKELNQLIDRTDLTYSDGMYDKETGIAAIAVKDKNTGETYIAYAGTNKDADGAT
                     DFISDANIGANNSAYLKRLGDKATSFYDRVQSNGDNITVTTGHSYGDFLASRVAIELI
                     PFFINFRKVNDSFHFCY"
     gene            877..1088
                     /locus_tag="VBE22_001985"
                     /pseudo
     CDS             877..1088
                     /locus_tag="VBE22_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285332.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1133..1456
                     /locus_tag="VBE22_001986"
     CDS             1133..1456
                     /locus_tag="VBE22_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203401.1"
                     /translation="MNNAAIKSLKNEVLELNPKNAFKGWGKLGRIAKAGKVLGWTGKI
                     VNIETNAKKDFFDDKTSTLDQKIKNFAIDSGVDALSGAAIGTLVGGPVGTVVGAVQGW
                     HLILL"
     gene            1542..>1733
                     /locus_tag="VBE22_001987"
     CDS             1542..>1733
                     /locus_tag="VBE22_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203402.1"
                     /translation="MKQSNHRYHGPLIEFEIEYSYIKIFPWIGLFISCIGLYASNFGD
                     PLGFIKFIFSVCIFVNLVSI"
BASE COUNT          644 a          212 c          341 g          536 t
ORIGIN      
        1 attaaaagta aaaagttatt tattaaaaca tccggaaact gagttgaaag atataacata
       61 tgaattgccg acacagtcga agcttattaa aaaatgagag ataaatttaa aatatctgat
      121 attaaggaag cagatattac ttttacaaaa agtggaaaaa ataattttga ctatacacta
      181 aggataaaaa ctaaaaacaa gagatactat ttgggtgaga cctatagttt tagaaaataa
      241 acggagaagt tatgggaaat gataatgtaa ctataaaact agaacatcgg gttaaaacat
      301 cagcagattt atcagattat gtatacaaat atgagagggc ttatacagat gctttaaata
      361 atggtgatga cataaaagaa gcaagtgaaa gtactaaaaa agagttaaat caactaattg
      421 atagaactga tttaacttat agtgacggta tgtatgacaa agagactggt attgcagcta
      481 ttgctgtaaa agataaaaat actggtgaga cttatatagc ttatgcgggt actaataagg
      541 atgctgatgg ggcaaccgac tttatttcag atgcaaatat cggtgctaat aactctgctt
      601 atttaaaacg attaggagac aaagctacta gcttttatga tcgtgttcaa agtaatggcg
      661 ataatattac tgtcacaaca ggtcattctt atggtgactt tctcgcttct agagtggcaa
      721 ttgagctaat tccattcttt attaacttta gaaaagttaa tgattcattt cacttttgtt
      781 attagcttgt gatcacttat aaagtgacag ctagttcatg aaatctctta ggagttttct
      841 ggcattaaag gtaacctatg atttaaaaaa tatctcatgg aattcaatgc ggaatcctca
      901 acagtctttt gtggactaac agaagttcag gatgtcgttt cggcaggcct taactattct
      961 cagaaggttt tggaaatttc aatggtacat ttgatacctc aggtaaggga ttagaatgga
     1021 caaaaacaat tcaaacaggt atgaaaaaac gagaagaaga tgctaaacag ttgagaaaaa
     1081 agctttagaa gattcaaata agctcagaaa aattgcgtca aaaggagtta ctttgaataa
     1141 tgcagcaata aaatccctta aaaatgaagt ccttgaatta aatcccaaaa atgcttttaa
     1201 aggatgggga aaattaggaa gaattgctaa agctggtaaa gtattaggtt ggactgggaa
     1261 aatagtcaat atagaaacta atgccaaaaa agactttttt gatgataaga cttctacact
     1321 agatcaaaaa atcaaaaact ttgctataga ttcaggtgta gatgcattgt ccggtgcagc
     1381 aataggaact ttggtaggag gacctgtggg aaccgttgtt ggagcagtgc agggctggca
     1441 tttgattttg ctttgaattt taaagttaag gagttgggta atgaatcaat tacaggtttt
     1501 gctaaaaaag gattaaaaga tttatttgga gggtaaaatt tttgaaacag agcaaccata
     1561 gatatcatgg accgttaatt gagtttgaaa tagaatattc atatattaaa atttttccat
     1621 ggattggact atttatatct tgcatagggt tatatgcaag taattttggt gatcctttag
     1681 gttttataaa attcatattt tctgtatgca tttttgttaa tttggtctca ata
//
LOCUS       DATRTN010000161         1685 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.162, whole genome shotgun sequence.
ACCESSION   DATRTN010000161 DATRTN010000000
VERSION     DATRTN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.162"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            84..614
                     /locus_tag="VBE22_001988"
     CDS             84..614
                     /locus_tag="VBE22_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO7203403.1"
                     /translation="MSFEHKHEGHDKHHDHREKYSLKTLKNISTKIDYKTALETAKKE
                     ARSGFISQIELNTKGDTPFYEIKVIDNKIAKKYKIDANSGKILSTTSDNQLNSKDAQL
                     KQPSKDIQTALQAIQAKYKNAKIKEISLANNKSNKLVYKVKLLDGNTPKIAQFDSDKA
                     SFIKANKLNTTHKNHD"
     gene            699..1370
                     /locus_tag="VBE22_001989"
     CDS             699..1370
                     /locus_tag="VBE22_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO7203404.1"
                     /translation="MKILVVEDEFDLNRSIVKLLKKQHYSVDSASNGEEALQFVSVAE
                     YDVIILDVMMPKMDGFTFLKLLRNKGSQVSILMLTARDAVEDRIAGLDFGADDYLVKP
                     FEFGELMARIRAMLRRANRQVSSDDIQIQDITINLSTKQVWRNDNLIDLTAKEYEVLE
                     YLARHRDQVLSRHQIREHVWDYDYYGESNIIDVLIKNLRRKLDNNRDGSLIKTKRGLG
                     YVIPK"
     gene            1354..>1685
                     /locus_tag="VBE22_001990"
     CDS             1354..>1685
                     /locus_tag="VBE22_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7203405.1"
                     /translation="MLFQSKINYPKKSSIITQITLWYSSFIFILVIGVLIGSFFISKS
                     IAENKSKKNLEAKAVQMSQALAKGHRYEAFEDGIFYSVYDQNGKVIYSGFPKGFKRDL
                     DHQHKHKK"
BASE COUNT          637 a          276 c          285 g          487 t
ORIGIN      
        1 tagcttccat tacgctactt ggtattgggg gagctatagg agtaagtgga gctgcattag
       61 cgcataacca tcactcagaa cacatgtctt ttgaacataa gcatgaagga cacgataaac
      121 atcacgatca tagagaaaaa tactcactaa aaacattaaa gaatatctcg acaaaaatcg
      181 attataagac tgccttagaa actgccaaaa aagaagctcg ctcaggcttt atttcacaaa
      241 ttgaattaaa taccaaaggt gatacacctt tttatgaaat caaagttatt gataataaaa
      301 tagctaaaaa atataaaatt gatgctaatt ctgggaagat cctttcaaca acatcagata
      361 atcaactcaa ttcaaaagat gcccaattaa aacaaccaag taaagatatt cagactgcat
      421 tacaagctat tcaagcaaaa tataaaaatg caaaaataaa agaaatttct cttgcgaata
      481 acaaatcaaa taagctagtt tataaagtaa aactacttga tggaaatact cctaagattg
      541 cccaatttga tagtgataaa gcatcattta taaaggcaaa taagcttaat acaactcata
      601 aaaatcacga ctaatactag tgtattaaag ggaataggac ctcagtactc cattcccttt
      661 tgtgatataa tggaaaaaag ccatacggag gccaaaaaat gaaaatcctt gttgttgaag
      721 atgagtttga cttgaaccgt agtattgtaa aacttttaaa aaagcaacac tacagtgttg
      781 acagtgcttc aaatggtgaa gaagccttac aatttgtttc agtggcagaa tatgatgtga
      841 ttatcttaga tgtaatgatg ccaaaaatgg acggcttcac ctttttaaaa ctgcttcgca
      901 acaaaggaag tcaagtatct attcttatgt tgactgctcg agatgccgtt gaagatcgta
      961 tcgcaggtct agattttggt gcggatgatt accttgtaaa accatttgaa tttggagaac
     1021 tcatggcccg aattcgagct atgttacgac gtgcaaatag acaagtatct tctgatgata
     1081 ttcaaattca agatataaca atcaatttat ctacaaagca agtttggaga aacgacaatt
     1141 tgattgattt aacagctaag gaatacgaag tccttgagta tttggcacgt cacagagacc
     1201 aagtcctctc tcgtcatcaa attcgtgaac acgtttggga ttatgattat tatggagagt
     1261 ccaatattat tgatgtcctt atcaaaaacc ttcgtcgaaa actagataac aatcgagatg
     1321 gatcactaat aaaaactaaa cgaggtttag gatatgttat tccaaagtaa aataaattac
     1381 cctaaaaagt ctagcatcat cactcaaatt accctctggt acagctcttt tatttttatc
     1441 cttgttatcg gtgtattaat tggttcattt tttatttcaa aaagcattgc tgaaaacaaa
     1501 tctaaaaaaa atttagaagc taaagctgtg caaatgagtc aagcgttagc taaaggtcat
     1561 cggtatgagg cttttgaaga tggaatattt tattcagtct atgaccaaaa tggaaaagtt
     1621 atctattctg gttttccaaa aggatttaaa agggatttag accatcaaca taaacataaa
     1681 aagaa
//
LOCUS       DATRTN010000162         1632 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.163, whole genome shotgun sequence.
ACCESSION   DATRTN010000162 DATRTN010000000
VERSION     DATRTN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.163"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..209)
                     /locus_tag="VBE22_001991"
     CDS             complement(<1..209)
                     /locus_tag="VBE22_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203406.1"
                     /translation="MAIKGITLKESMNRSVQAIMPLDEEKEVFNQKLVSYLTHLKDKE
                     DESEEYQKNLLKVFLESVLPYNFINT"
     gene            complement(450..815)
                     /gene="rplL"
                     /locus_tag="VBE22_001992"
     CDS             complement(450..815)
                     /gene="rplL"
                     /locus_tag="VBE22_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEO7203407.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAAAAA
                     SGEAAAAKDSFDVELTAAGDKKVGVIKVVREITGEGLKEAKAIVDNAPSVIKEGASEA
                     EANEIKEKLEAAGASVTLK"
     gene            complement(879..1379)
                     /locus_tag="VBE22_001993"
     CDS             complement(879..1379)
                     /locus_tag="VBE22_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEO7203408.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVEQDTNLRR
                     SLRESDVEFKVIKNSILIRAAEKAGLEDLKELFVGPSAVAFSNEDVIAPAKVISDFAK
                     DAEALEIKGGSVDGKFTSVEEINALAKLPNKEGMLSMLLSVLQAPVRNVAYAVKAVAE
                     KDEEVA"
     misc_feature    complement(1409..1542)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT          491 a          316 c          253 g          572 t
ORIGIN      
        1 gtatttataa aattgtaagg taatacactt tctaaaaata ccttgagaag atttttctgg
       61 tattcctctg actcatcctc tttgtctttt aaatgagtca aataactaac caatttttga
      121 ttaaatacct ctttttcttc atcaagtggc ataattgctt gaacagagcg attcatactc
      181 tcttttaatg taattccctt tattgccatg aaatagactc ctaaacagat ttatctaaga
      241 actgatagta ttagtctatt gtgtaagcaa tagactaagg gtacccttag cgatatatat
      301 acatattata tcataaataa aaccccacca agatttaatc ttggtggggt tttggtgggg
      361 aatgataaca ttctcccgat tttttatcgc tctaagcaat tctaaaacct tgatttacta
      421 ggcttccgca atcaaattgc gatactctat tatttaagag taactgaagc acctgcagct
      481 tcaagttttt ctttgatttc gttagcttct gcttctgaag caccttcttt aataactgat
      541 ggtgcgttgt caacaatagc tttagcttct ttaagacctt cgcctgtgat ttcacgaaca
      601 actttgataa cgccaacttt tttgtctcca gcagctgtta attcaacgtc aaatgagtct
      661 ttagcagcag cagcttcacc actagcagcg gcagcagcta caggagcagc agcagttaca
      721 ccaaattctt cttcgatagc ttttacaaga tcgttaagtt caagaattgt agcttcttta
      781 atttcagcaa taatattttc aatgtttaat gccattgtta tttcctccaa aaatatgttt
      841 taatataaaa ttatgtagct aggctacttg aagagtgctt atgccacttc ttcgtctttt
      901 tctgcaactg ctttgacagc atatgcaacg ttgcgtactg gcgcttgaag tactgaaagg
      961 agcattgaaa gcataccttc tttgtttggt aatttagcaa gagcgttaat ttcttcaact
     1021 gaagtgaatt taccgtctac tgaaccacct ttgatttcaa gagcttctgc atctttagcg
     1081 aaatcgctaa taactttagc tggtgcaata acatcttcgt tagaaaacgc aactgctgat
     1141 ggaccaacaa aaagttcttt aaggtcttca agacctgctt tttcagctgc acgaattaaa
     1201 attgagtttt tgataacttt aaactcaaca tcgctttcac gaagtgaacg acgtaaatta
     1261 gtatcttgct caacagtaag accacgtgaa tcaacaacaa cgatacttgc tgctgctttc
     1321 attttctcag caataagttc aacttgttca gctttttttg caataattgc ttcactcatt
     1381 agttttacct ccgtttttat tttttgggat aggcacaaaa aaatcgcacc taaacctaga
     1441 cacgaaagta caatacgtta ttttataatg acgttttgtg cctcggtagg aatattatga
     1501 gcactgctcc cctactgtct taggtcagtt tcatcagaaa ctttaaatag tataacttat
     1561 aattctagca ttgtcaataa tttttattat ttttctgaaa taattttata gtaccaaaaa
     1621 atactaaaat ag
//
LOCUS       DATRTN010000163         1570 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.164, whole genome shotgun sequence.
ACCESSION   DATRTN010000163 DATRTN010000000
VERSION     DATRTN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.164"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..>1570
                     /gene="leuS"
                     /locus_tag="VBE22_001994"
     CDS             <1..>1570
                     /gene="leuS"
                     /locus_tag="VBE22_001994"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEO7203409.1"
                     /translation="DPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVEELGTAIANEEVL
                     PDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKDMQRNWIGKSTGA
                     NVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQAEAVAEYKRQAS
                     LKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPVWIADYVLASYGTGAIMAVPAHDER
                     DWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASAIAKMVEWLEAEG
                     VGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLVLPVTKDIRPSGT
                     GESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPHNTEKLADEELLK
                     QWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFNQGMILGTSYRDS
                     RGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDVVEQYGADTLRVY
                     EMFMGPLDASIAWS"
BASE COUNT          439 a          363 c          376 g          392 t
ORIGIN      
        1 ctgaccctaa ctactacaag tggacacagt ggatctttac taagctttat gaaaaaggcc
       61 tagcctatga agctgaagtt cccgttaact gggttgaaga attgggaacg gccatcgcca
      121 acgaagaggt tcttccagat ggcacatcag aacgtggcgg ctacccagtt gtcagaaaac
      181 caatgcgcca atggatgctc aaaatcactg cctatgcaga gcgcttgcta gaagacttgg
      241 aagaagttga ctggccagaa tcaattaagg acatgcaacg caactggatt ggtaaatcaa
      301 ctggtgccaa tgtaaccttc aaggttaagg atacagataa ggacttcact gtcttcacaa
      361 cacgtcctga cacccttttt ggtgcaactt acgcagttct tgctcctgaa catgccttgg
      421 ttgatgctat tacaacagct gaccaagcag aagctgttgc cgaatacaaa cgtcaagcaa
      481 gtcttaaatc agatcttgcc cgtacagacc ttgctaaaga aaaaacaggt gtctggacag
      541 gtgcctatgc tattaaccca gttaatggta aagaaattcc agtttggatt gcggactatg
      601 tccttgccag ctacggtaca ggtgctatca tggctgttcc agcccacgat gaacgtgact
      661 gggaattcgc aaaacaattt aagctagaca tcattccagt tcttgaaggt ggtaatgttg
      721 aagaggctgc ctttacagaa gacggtcttc acatcaactc agacttccta gatggtctcg
      781 ataaagcgtc agctattgcc aaaatggtcg aatggcttga agcagaaggt gttggtaatg
      841 agaaagtcac ttaccgtctc cgcgactggc tcttcagccg tcaacgttac tggggtgagc
      901 caatcccaat catccactgg gaagatggga catcaacagc ggttcctgag agtgaattgc
      961 cactggttct tccagtaacc aaggatatcc gtccttcagg tactggtgag agcccacttg
     1021 ctaacttgac agactggttg gaagtgactc gtgaagatgg tgttaaaggt cgtcgtgaga
     1081 ctaacaccat gccacaatgg gcaggttcaa gctggtacta tctccgttac attgatccac
     1141 acaacaccga aaaactggct gatgaggaac ttctcaaaca atggttgcca gttgatatct
     1201 atgtcggtgg tgcagagcat gccgtgcttc accttctcta cgctcgcttc tggcacaagg
     1261 ttctttacga tctcggtgtc gtacctacca aagagccatt ccaaaaactc tttaaccaag
     1321 ggatgatttt gggaacaagc taccgcgaca gccgtggtgc acttgtggca actgacaagg
     1381 ttgaaaaacg tgatggtagc ttcttccatg tggaaactgg ggaagagctt gaacaagcac
     1441 cagctaagat gtctaaatca cttaagaacg ttgtcaaccc agatgatgtt gttgaacagt
     1501 acggtgctga cacccttcgt gtttacgaga tgttcatggg accacttgac gcttcaatcg
     1561 cttggtctga
//
LOCUS       DATRTN010000164         1544 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.165, whole genome shotgun sequence.
ACCESSION   DATRTN010000164 DATRTN010000000
VERSION     DATRTN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.165"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            107..511
                     /locus_tag="VBE22_001995"
     CDS             107..511
                     /locus_tag="VBE22_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000479532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazolinyl imide reductase"
                     /protein_id="HEO7203410.1"
                     /translation="MFLMHKISLTTNSGSLTLSGTNGPIIWEPCLDKPTDENNRFNLE
                     KNEFSELKIFEITEEVEETYNDMMKLSWVEAISKSVIDFTNNIEAEKVDLREQQYLIS
                     AIEAWRALSRELGQSNTIQPYKKTAIKMEDLI"
     gene            549..1118
                     /locus_tag="VBE22_001996"
     CDS             549..1118
                     /locus_tag="VBE22_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEO7203411.1"
                     /translation="MKDLIMIGVFSALYFLFNMVGGIPFAMNPVLTFYQPMGSAFLSG
                     IIFMFLIAKAPKKGTISILAIIMCILRLATGMHWAMAVGTLITGIVAELIAGSKSYKN
                     KKMNMLSFGVFALGDIGTFLVYFMNPKSWSDAMIKKGTDITYIESMNAAAADWMIYVI
                     VIGTFLVALLSAWIGMKLLKKQFEKAGIV"
BASE COUNT          545 a          201 c          291 g          507 t
ORIGIN      
        1 aaaatttaat agaaaaagga ttaagctcta ttaaaattat gcaaacaata ttccgatatg
       61 cataagtgtt caaaatcaaa tgacttctaa aaacccggac aattatatgt tcttaatgca
      121 caaaatttcc cttacaacaa attcaggaag tttaacatta tccgggacaa acggtcctat
      181 aatatgggag ccgtgtttag ataaaccaac agatgaaaat aacagattta atttagaaaa
      241 aaatgagttt tcggaattaa aaatatttga aataacggag gaggttgaag aaacatataa
      301 cgatatgatg aaattaagtt gggtagaggc catatctaaa tccgtgattg acttcaccaa
      361 caatatcgag gcagaaaaag ttgacttaag agaacagcaa tatttgattt cagcaataga
      421 ggcatggaga gcattatcta gggagttggg tcaaagtaat accattcaac cttacaaaaa
      481 aactgcaatt aaaatggaag atttaatata aggaggaaat tatgaataac aaagaaaaca
      541 aattataggt gaaagattta ataatgattg gtgtattttc cgcattatac ttcttgttta
      601 atatggtagg aggaatacct tttgctatga atccggtgct aacattttat caaccgatgg
      661 gaagtgcatt tttatcagga ataatattta tgtttttgat cgcaaaagca cctaaaaaag
      721 gaacaatttc tattttagca atcatcatgt gtattttaag gctggcaaca ggaatgcatt
      781 gggctatggc ggtaggaact ttaataacag gaatagtagc tgagcttatt gcaggaagta
      841 agagttataa aaacaagaaa atgaacatgt tgtcttttgg agtttttgca cttggagata
      901 tcggaacatt tttagtgtat tttatgaatc ctaagtcttg gtcagatgcg atgataaaaa
      961 aagggacaga tatcacctac attgagtcta tgaatgctgc agctgctgat tggatgattt
     1021 atgtaattgt tattggaact ttcttggttg ctttattaag tgcttggatt ggaatgaagc
     1081 ttttgaaaaa acaatttgaa aaggccggca ttgtataatg agaaaagtat caaatgttca
     1141 atttaaccct tgcacaaaaa tagttttact atttttgtgc ataatcattt caagtttatt
     1201 atcttcaata gaattggagc taatttttgt atttataatt gcgttatttt gcattataaa
     1261 caaaaagatt aaatttgctc ttaatgggtt tctgatttac ctaggtgtat attttgttag
     1321 cattttgact gtgcagtacg gtagcaatac cttaagaagc ttattaatgc cttttctagg
     1381 tttagtacat aagctttacc cggtgtgcat gttttctgtg ctaatactaa gtactacaaa
     1441 agtaggtgaa tttttatctg ctatggaatt tgcaggtgtt tctaaaaaaa tcacaattcc
     1501 tttagctgta atgcttagat atattccaac cgtgcgtgaa gact
//
LOCUS       DATRTN010000165         1420 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.167, whole genome shotgun sequence.
ACCESSION   DATRTN010000165 DATRTN010000000
VERSION     DATRTN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.167"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..590
                     /locus_tag="VBE22_001997"
     CDS             <1..590
                     /locus_tag="VBE22_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO7203412.1"
                     /translation="AKRFLDITGAIIGLLICGIVAIFLVPQIRKDGGPAIFSQNRVGR
                     NGRIFRFYKFRSMRVDAEQIKKDLLVHNQMTGLMFKLDDDPRITKIGKFIRKTSIDEL
                     PQFYNVLKGDMSLVGTRPPTVDEYEKYNSTQKRRLSFKPGITGLWQISGRNNITDFDE
                     IVKLDVQYINEWSIWSDIKIILLTLKVVLLGTGAK"
     gene            614..1063
                     /locus_tag="VBE22_001998"
     CDS             614..1063
                     /locus_tag="VBE22_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEO7203413.1"
                     /translation="MKICLVGSSGGHLAHLNLLKPIWEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     VYIEVFDRIDKPTLTGKLVYPVTDKFIVQWEEMKKVYPKAINLGGIF"
     gene            1063..>1420
                     /locus_tag="VBE22_001999"
     CDS             1063..>1420
                     /locus_tag="VBE22_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEO7203414.1"
                     /translation="MIFVTVGTHEQQFNRLIKEVDRLKGTGAIAQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNSYMKEAEIVITHGGPATFMNAVSKGKKTIVVPRQEQFGEHVNN
                     HQVDFLKELFLKYELDY"
BASE COUNT          485 a          190 c          298 g          447 t
ORIGIN      
        1 tagcaaaacg atttttggat atcacgggtg ctattatagg tttgctcata tgtggcattg
       61 tggcaatttt tctagttcca caaatcagaa aagatggtgg accggctatc ttttctcaaa
      121 atagagtagg tcgtaatggt aggattttta gattctataa attcagatca atgcgagtag
      181 atgcagaaca aattaagaaa gatttattag ttcacaatca aatgacaggg ctaatgttta
      241 agttagacga tgatcctaga attactaaaa taggaaaatt tattcgaaaa acaagcatag
      301 atgagttgcc tcaattctat aatgttttaa aaggtgatat gagtttagta ggaacacgcc
      361 ctcccacagt tgatgaatat gaaaagtata attcaacgca gaagcgacgc cttagtttta
      421 agccaggaat cactggtttg tggcaaatat ctggtagaaa taatatcact gattttgatg
      481 aaatcgtaaa gttagatgtt caatatatca atgaatggtc tatttggtca gatattaaga
      541 ttattctcct aacactaaag gtagtcttac ttgggacagg tgctaagtaa aggtaaggtt
      601 tgaaaggaat ataatgaaaa tttgtctggt tggttcaagt ggtggtcatc tagcacactt
      661 gaaccttttg aaacccattt gggaaaaaga agataggttt tgggtaacct ttgataaaga
      721 agatgctagg agtattctaa gagaagagat tgtatatcat tgcttctttc caacaaaccg
      781 taatgtcaaa aacttggtaa aaaatactat tctagctttt aaggtcctta gaaaagaaag
      841 accagatgtt atcatatcat ctggtgccgc tgtagcagta ccattctttt atattggtaa
      901 gttatttggt tgtaagaccg tttatataga ggttttcgac aggatagata aaccaacttt
      961 gacaggaaaa ttagtgtatc ctgtaacaga taaatttatt gttcagtggg aagaaatgaa
     1021 aaaagtttat cctaaggcaa ttaatttagg aggaattttt taatgatttt tgtcacagtg
     1081 gggacacatg aacagcagtt caaccgtctt attaaagaag ttgatagatt aaaagggaca
     1141 ggtgctattg ctcaagaagt gttcattcaa acgggttact cagactttga acctcagaat
     1201 tgtcagtggt caaaatttct ctcatatgat gatatgaact cttacatgaa agaagctgag
     1261 attgttatca cacatggcgg tccagcgacg tttatgaatg cagtttctaa agggaaaaaa
     1321 actattgtgg ttcctagaca agaacagttt ggagagcatg tgaataatca tcaggtggat
     1381 tttttgaaag agttattctt gaaatatgaa ttagattata
//
LOCUS       DATRTN010000166         1415 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.168, whole genome shotgun sequence.
ACCESSION   DATRTN010000166 DATRTN010000000
VERSION     DATRTN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.168"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            42..620
                     /locus_tag="VBE22_002000"
     CDS             42..620
                     /locus_tag="VBE22_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7203415.1"
                     /translation="MSNLDILYCKLAMCTTLLTERLRLQPVDLSNINDFLEFSSDSET
                     VFYMQRYKANTTEEAQMVLANVCMKSPLGIYAMIETESNKMIGIIELEIRDEFSAEFG
                     YILNKNYSGKGYMTEACSKLMSVGFEYLDLERIYARFDINNKKSGNVMERLGMKKEGE
                     LRHLAKNPKGEWKTRAYYSILKEEYFNKVNTK"
     gene            663..1343
                     /locus_tag="VBE22_002001"
     CDS             663..1343
                     /locus_tag="VBE22_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7203416.1"
                     /translation="MGDVVENFTEGKNPKIDTLNGKTVRIEKINPDHFEDLFQVYGEL
                     STEDSLTYISFSKFNSKNEFDVFFQTLLKSEDPYYLAIVDNNTGKALGTFSLMRIDTK
                     NRVVEMGWVVYSSKLKQTRIATEAQYLVMKYVFEELCYRRYEWKCDSLNAPSNNSAKR
                     LGFTFEGTFRQAVVYKGRNRDTNWYSILDKEWPEKKTRFEKWLDDSNFDVNGYQIRSL
                     SSIEQKTK"
BASE COUNT          535 a          159 c          261 g          460 t
ORIGIN      
        1 aagtggttta tagataatta aggaaggaat attggttaga aatgagtaat ttggatatac
       61 tatattgtaa gttagcaatg tgcactacat tactaacgga aagactacga cttcagcctg
      121 ttgatttatc taatataaat gactttttag aattttcatc tgattctgaa accgtatttt
      181 atatgcaacg atataaagcc aataccacag aagaagcaca aatggttttg gcgaatgtct
      241 gtatgaaatc accattagga atctatgcga tgattgagac agaatcaaat aaaatgattg
      301 gtattataga attagaaatt agagatgagt tttcagcaga attcggttat atattaaata
      361 aaaattatag tggtaaaggt tatatgacag aagcttgttc aaaattaatg tcagttggtt
      421 ttgaatatct agacttggaa agaatttatg ctcgtttcga cattaataat aaaaagtctg
      481 gaaatgttat ggaacgttta ggtatgaaaa aagagggcga acttcgtcat cttgcaaaaa
      541 atccaaaagg tgagtggaaa acaagagcgt attattctat tttaaaagaa gaatatttta
      601 ataaagttaa tactaaatag tctgagatgt agagaattag atataatctt tttaatcagc
      661 cagtgggtga tgtcgttgaa aattttacgg aaggtaaaaa tccgaagatt gatacactta
      721 atgggaaaac agttagaata gaaaaaataa atccagatca ttttgaagac ttatttcaag
      781 tttatggaga attgtctact gaagatagtt taacttatat atcttttagc aaatttaata
      841 gtaagaatga atttgatgtc tttttccaaa ctctattaaa atcagaagat ccttattatt
      901 tagctattgt tgataataat actggtaaag cattagggac tttttcatta atgcgtattg
      961 atacaaaaaa tagagttgtt gaaatgggat gggtggttta ttcatcaaaa ctaaaacaaa
     1021 caaggatagc aacagaagct caatatttgg ttatgaaata tgtttttgaa gaactctgtt
     1081 accgccgtta tgagtggaaa tgtgatagtt tgaatgcacc atctaataat tctgcaaaac
     1141 gacttggttt tacttttgaa ggaactttta gacaagctgt cgtatataag ggtcgtaaca
     1201 gagatacaaa ttggtattct attttagata aagagtggcc agagaaaaaa acacggtttg
     1261 aaaaatggct agatgattca aactttgatg taaatgggta tcaaatcaga tcactatcaa
     1321 gcattgagca gaagacaaaa tagagtcatt cattaatcac taaaatttca aaattacatg
     1381 tttttttagc taacatctta aactttgtta aataa
//
LOCUS       DATRTN010000167         1388 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.169, whole genome shotgun sequence.
ACCESSION   DATRTN010000167 DATRTN010000000
VERSION     DATRTN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.169"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..>1388
                     /locus_tag="VBE22_002002"
     CDS             <1..>1388
                     /locus_tag="VBE22_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="surface protein"
                     /protein_id="HEO7203417.1"
                     /translation="VQINAIHLQTALDKVRTGEFESKDFTHYTFDSIKRKYQVSGKKV
                     LMDNNYRFGSGIKNTRQDAIAIVRLLLEKNKDYLIYMNGLEEPSVLYNPENKRYKFTN
                     NKGKAIGNIPVGLTAFENSAETQEYVLKNIQKNETIYLGNTRVDNPRVTVNNKKRDTI
                     GVEYGKRITYRIPIYSKQLTVRVSPNFVVDSTNYNYRLSQAPIIAEEGKEGRLYPIDS
                     SISVDGDKIVLNSSGDETEQKELLQNSLYKLHSIDKLDFDLTNADIKELEITGHVASK
                     RDYNLSVAQEKKGVIETKRSLISVDNQKQDQGIFVEDSKGYIKTPSLSSYGINFAMYD
                     DNQNRLLQGSEYILGRLNADKKVSLYQPNGFWINTQKTIKSLNKEYLNQNAYHIRGNN
                     IIGIDGYSNDIDLNPRLWAYNYKKQVKSNQSLFKLRGLSNKYKYFLIQTKSPKGYLQS
                     NKPFYFTVNKDS"
BASE COUNT          538 a          174 c          256 g          420 t
ORIGIN      
        1 tgttcagatt aatgctattc atttacaaac agcattggat aaagtgagga cgggggagtt
       61 tgagagcaaa gactttacac actatacttt tgatagtatc aagcggaagt accaagttag
      121 tggtaaaaaa gtccttatgg ataataacta tagatttggt tctggtataa aaaatactag
      181 acaggatgct attgctattg taaggttact gcttgaaaag aataaagatt atttaattta
      241 catgaatggc ttagaagagc caagtgtttt atataatcca gaaaataaac gttataagtt
      301 cacaaataac aaaggaaaag caattggtaa tattccagtt ggtttaactg cttttgaaaa
      361 cagcgcagaa actcaagaat atgtgttgaa aaatattcag aaaaacgaaa cgatttatct
      421 tggtaatacc cgagtagata atcctcgagt aactgttaat aacaaaaaaa gagatactat
      481 aggagttgaa tacggtaaac gtattactta taggattcca atttattcaa agcagctgac
      541 tgtcagagtt agccctaatt ttgtagtaga tagtactaat tataattacc gactttctca
      601 agcgcctatt attgcggaag aaggtaaaga gggacgtttg tatccaatag atagcagtat
      661 tagtgtcgat ggtgataaaa ttgttttaaa tagtagtggt gatgaaacag agcaaaaaga
      721 attattacaa aatagcctgt ataaacttca ctctattgat aagttagact ttgatttgac
      781 aaatgctgat attaaagagt tagagattac aggacatgtt gcttcaaaaa gagattataa
      841 tttgtctgtt gctcaggaaa agaaaggcgt catagagact aaacggagtc taatttctgt
      901 tgataatcaa aaacaagatc aagggatttt tgtcgaggat tctaaaggct atataaagac
      961 acctagtttg agcagttatg gtataaattt tgccatgtat gacgacaatc aaaatagatt
     1021 attgcaaggt tcagagtata ttttaggaag attgaatgca gataaaaaag tatctctata
     1081 tcaacctaat ggcttttgga taaatactca aaaaacgatt aagagtttaa ataaagaata
     1141 tcttaaccaa aatgcttacc atattagagg aaataacatt ataggtattg atggctattc
     1201 aaatgacata gacttgaatc ctaggttatg ggcttataac tataaaaaac aagtgaaaag
     1261 taaccaatca ttatttaaat tacgaggttt gtctaataag tataagtatt tcttaataca
     1321 gacaaaatct ccaaaaggct acttacaaag caataaacca ttttatttta cagtaaataa
     1381 agatagtg
//
LOCUS       DATRTN010000168         1353 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.170, whole genome shotgun sequence.
ACCESSION   DATRTN010000168 DATRTN010000000
VERSION     DATRTN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.170"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            1075..>1353
                     /locus_tag="VBE22_002003"
     CDS             1075..>1353
                     /locus_tag="VBE22_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7203418.1"
                     /translation="MFSKKLKELRINNKLSQKQIAEQMNISQQAYAKWESGKNSPSLA
                     TLEKVANFFNLSIEELLSDGTVSLDNLLKAETITYQGKTLSEEDAFKVK"
BASE COUNT          472 a          238 c          182 g          461 t
ORIGIN      
        1 ttttcaaaaa taaaatatag tttagtaaag gacgaaacct cgcattctaa actatatttt
       61 ttatttcttt ttgcaaacaa aaaagccgac ttgtgaaaga agtcgacata gaaaaaaatt
      121 tctaaataaa ttacaacacg aaaggtaaat aataacttac cttataaaaa tttaaaaaat
      181 atgctcttat cttcgcatac tactttctca atgtttcaca cataggtact cgttcgaata
      241 agtccacctt attcattagc tagatagtat ctaacacacc gcccacaata ccatacctta
      301 tccgcctctc tacgttttta gtaatcattg tcaacacccc ttgcgaggta gcagttttag
      361 ttttttgctc aacacaggct aactaacata aaccttagtg tgcttttgag gtctgaactt
      421 tacttgttta gtcatcacta gaatgataaa atatatttgg tgattctaca tcatgcccaa
      481 ctccttttag atctacctga aagcaaactt tgaacagaat tgactctcca gagttagtct
      541 aataaggagg tagggtatga tagaaattat caccgaagtg atgtttgcta tcatgaaagg
      601 attcttccaa ttacttggat tatactttca ttgcttagtt gaacacacag taattactat
      661 aatcataact gcaattgttt tgcatcaatt tttatagtaa ccatttgcta gatagtcttc
      721 tatctagctt tttttgctta aacaagcaaa gttcagacct caaaagcaag ccagacaact
      781 tgtgtagtat actacacctg ctttactttt gaagaatttc acgttataat aaacttgcta
      841 gaatttatta cgtgtgttat tcaacttagc ttagctgtgt tgtctgacgc actttttatt
      901 taattatcaa tgggcaacct atttagtcac tcttaatttc tatacgatgt tgtcttttgc
      961 taattattaa ttgctcaaca tcaaatagaa agaattgact aacaaccata tcgttgttat
     1021 aatcatttta caaccttata gttgttttgt caataggtaa attcaagaaa ttttatgttt
     1081 tccaaaaaac taaaagaatt aagaattaac aacaagcttt ctcaaaaaca aatagctgaa
     1141 caaatgaata tttctcaaca agcttatgca aaatgggaaa gtggaaaaaa ttctccatct
     1201 ctcgcaacac ttgagaaagt tgcaaatttc ttcaatttat ctattgaaga attactatct
     1261 gatggtactg ttagtcttga caacttatta aaagctgaaa ctattactta tcaaggaaaa
     1321 acactatcag aagaagatgc ctttaaagta aaa
//
LOCUS       DATRTN010000169         1347 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.171, whole genome shotgun sequence.
ACCESSION   DATRTN010000169 DATRTN010000000
VERSION     DATRTN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.171"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(1..510)
                     /locus_tag="VBE22_002004"
                     /pseudo
     CDS             complement(1..510)
                     /locus_tag="VBE22_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            688..888
                     /locus_tag="VBE22_002005"
     CDS             688..888
                     /locus_tag="VBE22_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO7203419.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFIEKTIAKGK
                     ELADDAKDAVEGAVDAVKEKLK"
     gene            930..1118
                     /locus_tag="VBE22_002006"
     CDS             930..1118
                     /locus_tag="VBE22_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO7203420.1"
                     /translation="MSIRTDIDEFKEAIDKGYISGNTVAIVRKNGKIFDYVLLHEEVR
                     EEEVVTVERVLDVLRKLS"
BASE COUNT          473 a          174 c          261 g          439 t
ORIGIN      
        1 atttttttgg ttgatttgtc tatgctttaa atacttgaaa aatgcttcag ttacggcatt
       61 atcataagga tatctaggtt tagaaaaaga atgcatgata ttgttgtcat caattatttt
      121 tctaaattcc ctggccttaa attgtgaccc ttggtcggaa tgaaagagaa gtgttccttc
      181 aatctttctt ttattaagag ctaattctag agtgtcacat gctaactttg catccatacg
      241 atgactcagt ttccaagcaa tatattttct agaatagaga tcaattattg cgcagagata
      301 gacatatttc ttatatccaa tagaaatata ggtgaagtca gtagaccata cttgattagg
      361 tttatcagga ttaaatttct ggttgagtaa gttttgagga tacgtttttt gagtcttttt
      421 aagagctgtt tttgtcttaa cggtagccat tttagggagc gccatatcct tcatcagacg
      481 gtaaattctt ccttcagaga tttttgtgtc ttttaaaaca ggttataaca cacaaagtgt
      541 tgtctatttt tatagtatac atccatactc gcataaccat gagataatca ctaaaacaag
      601 agtcgcctga cactagcgac tcttgttttt atttgctaaa gaaagatata gatgttatga
      661 ttaataaaaa taataaggag gtgcagtatg tcacaagaaa aactaaaatc aaaattagat
      721 caagcaaaag gtggtgctaa agaaggcttt ggcaaaataa ccggtgataa agagttagaa
      781 gcaaaaggat ttatcgaaaa aacaattgct aaaggcaaag aactagcaga tgatgctaaa
      841 gatgctgttg aaggggcagt agatgctgtc aaagaaaaac tgaagtaaca aataaccgct
      901 catttaacga gcggttattt gttatctttt tgtctatcag aacagatata gatgagttta
      961 aagaagcgat tgataaaggc tatatttcag ggaacacagt agcgatagtg cgtaaaaacg
     1021 gaaagatatt tgattatgtg ttactacacg aagaagtgag agaagaagag gttgttacag
     1081 ttgagagagt gcttgatgta ctgaggaagt tatcataata acggaccaaa attcgcccca
     1141 aaaagtattt taaaatagaa taaaataaaa aaatatgctt tttattatgg atgtatcgtg
     1201 tattttaacc tttaatatat cctattttaa tggtagagaa gttggcaggg gacatattaa
     1261 aacctttttt gagatgttag gaaggatttt attctttaga ctagtgttga gaaaataaca
     1321 aaacttaaat aagattattt aagtttt
//
LOCUS       DATRTN010000170         1316 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.172, whole genome shotgun sequence.
ACCESSION   DATRTN010000170 DATRTN010000000
VERSION     DATRTN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.172"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..448)
                     /locus_tag="VBE22_002007"
     CDS             complement(<1..448)
                     /locus_tag="VBE22_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEO7203421.1"
                     /translation="MKDFIKQSDTTFVRIIKSLLIGGFIGAILGSVGALFIIFGQNKY
                     LSEINIIQYFLWVSRIVVIITALFSLIYLYQIQKYQKVFFNVDESQSEEIYRQINLRH
                     SYGMTFVSISIVLSIVNTLFNYKLNIFDDSVTLVIPIYDLSLLFVLW"
     gene            complement(451..666)
                     /locus_tag="VBE22_002008"
     CDS             complement(451..666)
                     /locus_tag="VBE22_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO7203422.1"
                     /translation="MGLILKNRLKELRARDGINQTELAKLTGVSRQTISLIERGEYTP
                     SVIIAMKVSQVFKESLENVFSLVSTEE"
     gene            complement(711..993)
                     /locus_tag="VBE22_002009"
                     /pseudo
     CDS             complement(711..993)
                     /locus_tag="VBE22_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245557.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          447 a          257 c          159 g          453 t
ORIGIN      
        1 cccataaaac aaatagcagt gataaatcat atattggaat aactaaagta actgaatcat
       61 caaaaatatt tagtttatag ttaaataaag tattaactat actgagtact attgaaattg
      121 acacaaaagt catcccataa gaatgtctta aattaatctg gcgataaatt tcttcagatt
      181 gactttcatc aacattgaaa aaaactttct gatatttttg aatctgatag aggtagatga
      241 gactaaagag agctgttata attacaacaa tacgactaac ccataggaaa tattgaataa
      301 tattaatttc agataagtac ttattttgac caaatatgat aaaaagagct cctacggaac
      361 caagtatggc ccctataaaa ccaccaatta aaaggctttt gataattcga acaaatgtag
      421 tatcactttg ttttataaaa tctttcatcg ttattcctcc gttgacacta aactaaaaac
      481 attctctagg gattctttaa atacttgaga aaccttcata gcaataataa cagatggcgt
      541 atattctccg cgttcaatta ggctaattgt ttgtcttgag actcccgtta atttggcgag
      601 ttctgtttga ttgataccat ctcgtgcacg caattctttg agcctatttt ttaaaattaa
      661 tcccatcatt tttctccttt ataaaatgat atatagattt atccctcgct ctatttattg
      721 actggacgca attccttgat atcatgaata cggacaatac gtacctcctc tttacttcgt
      781 ttcaagttgc gacgaatctg tacccaatcc tcatctacct ttaaaatctc ataaggatgc
      841 gtttgaaaac catcctctaa ataaatcata catgtgcttc ctactaaatc ttgaatcatt
      901 tgtttttcct ccctgattta ttttttctaa ttgtttaact ctacgactga gtgccttttg
      961 ctctttaggt acaccaatat acatcatgac cataaaaaaa gccattaagc caaaaaattc
     1021 catctatcta ctccctttct ttttgcaaac aaaactcaag ccctgcaatt gatgctacca
     1081 acacacaaaa tacaatttca tttacttcta ttatatcaag catatcaaga gcaagattac
     1141 tcaccataca taagcaaact agggtttcca taactcttgc aaccatccga cctgtaataa
     1201 taaattcctt ttcatttgac taactcctaa aaccattgtg cttttgaaac aatgattaat
     1261 ttgattgtta aatgattaag tgattttaac acgcttctcc tttgatatta tttttc
//
LOCUS       DATRTN010000171         1308 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.173, whole genome shotgun sequence.
ACCESSION   DATRTN010000171 DATRTN010000000
VERSION     DATRTN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.173"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            120..686
                     /locus_tag="VBE22_002010"
     CDS             120..686
                     /locus_tag="VBE22_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO7203423.1"
                     /translation="MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKD
                     AKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSAEYSYHVDAKTGQILERDMDNNGFSK
                     STSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVYDIDFVD
                     LKNKNEVDYQIDAETGKIIERSRDHMND"
     gene            complement(735..>1308)
                     /locus_tag="VBE22_002011"
     CDS             complement(735..>1308)
                     /locus_tag="VBE22_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO7203424.1"
                     /translation="SILLSMFFGTLLAIMRNSKNPIWKLIASIYIEFVRNVPNLLWIF
                     IIFLVFQMKSVSAGITSFTIFTSAALAEIIRGGLNGVDKGQTEAGLSQGFTYLQVFII
                     IIFPQAFRKMLPAIISQFVTVIKDTSLLYSVIAIQEIFGKSQILMGRYFEAGQVFTLY
                     AIITAVYFITNFIISSFSRKLSKRWEQATE"
BASE COUNT          501 a          176 c          237 g          394 t
ORIGIN      
        1 tgaggtatta atgtcaaatg gtttttattg accacatgtt tttatgaacg agtggtcaaa
       61 attgaaaaag tgtctagctt atgttaaaat ataattgaat aatagaaaga aggtgctgta
      121 tgattaaaaa aaataaagtt tttttaggag ttcttttagt ccttgttgtt atccttggag
      181 gtggtgtatt attttaccaa tcacaattcc aaaagacaac taatcaagcg ttagctattg
      241 cttataaaga tgctaaagtg gctaagaagg atgtcataca tcaaaaaatt gacaaagaat
      301 ttgaaaactt tagagggagc tacgaaattg agttcaatac caaaagcgca gaatacagct
      361 atcatgtaga tgctaagact ggacaaatcc ttgagcggga catggataat aatggctttt
      421 caaaatcgac ttctcagtca agttcatcgt caagtcaaaa atctcataaa ataagtcaag
      481 aagaagccaa aaagattgca tttaaagatg ctaatataga ggaatcagaa gtcagtaatc
      541 ttaagatcaa agaagaaatt gaaaatggaa aatcagttta tgatattgac tttgtggacc
      601 tgaaaaataa aaatgaagtt gattatcaaa ttgatgcaga aactggaaaa atcattgaac
      661 gttctagaga tcatatgaat gattaattta aataagatat aaaaaaacga aggtctaaaa
      721 ggccttcgtt ttaattactc agtagcttgc tcccaacgtt ttgaaagttt acgtgaaaaa
      781 cttgagatga taaagtttgt aatgaaatag acagcagtta taatagcgta gagtgtaaaa
      841 acttgtccag cttcgaaata tcttcccatt agaatttgac tttttccgaa gatttcttga
      901 atagctataa cagaataaag tagtgatgta tcttttataa ccgttacaaa ctgagaaata
      961 atggctggta acattttgcg aaaagcttgt gggaaaataa taataatgaa aacttgcaaa
     1021 taagtgaatc cttgagaaag tccggcttct gtctgtcctt tatcgactcc atttaaacct
     1081 ccccgaataa tttctgctaa agcagcggag gtaaaaattg taaacgaagt aataccagcg
     1141 ctgactgatt tcatctggaa tactaagaaa atgataaaaa tccaaagtag atttggaaca
     1201 tttctgacaa attcgatata gatactagct attaatttcc agataggatt tttgctattt
     1261 ctcataattg ctaatagagt tccaaaaaac attgatagta aaatggaa
//
LOCUS       DATRTN010000172         1261 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.174, whole genome shotgun sequence.
ACCESSION   DATRTN010000172 DATRTN010000000
VERSION     DATRTN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.174"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            262..>1261
                     /locus_tag="VBE22_002012"
     CDS             262..>1261
                     /locus_tag="VBE22_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO7203425.1"
                     /translation="MNIIIGTSLLILVLAIFTLFNYKAPYGTKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVTPGYAVLIGLSVSGTG
                     IIPGFLAGYLVGFLVKWMERNIPGGLDLISIIIIGAPLTRLVAKLLTPLINSTLLTIG
                     DILTSGAHSNPILMGIILGGTIVVVATAPLSSMALTAMLGLTGMPMAIGALSVFGSSF
                     MNGVLFHKLKLGSRKDNIAFAVEPLTQADVTSANPIPIYVTNFVGGAACGILIALMKL
                     VNDTPGTATPIAGFAVMFAYNPMIKVLITALGCIILSLLAGYFGGIVFKDYKLVTKE"
BASE COUNT          357 a          203 c          253 g          448 t
ORIGIN      
        1 cgtaataaaa aaaagttaag aaaataaaaa attattattg actatggtta tgcttttagg
       61 taaaatgtga ttatattagc aactaaggag taatccatga gataagccta ataattatgt
      121 cggacttctt tgttgcacct tatccagttc gtagtacaat taagtttcag tattacctaa
      181 aggcgccttt attgaaattg aagcagtagt acacagacac tgagaataaa aaacttaact
      241 atttttagcg ggagtatatt tatgaatatc attattggga ccagtctttt aatacttgta
      301 ttagcgattt tcactttatt taattataaa gcaccctacg gaacaaaagc aatgggagca
      361 ttggcatctg cggcttgtgc atccttcctt gtagaagctt ttcaagattc tttctttggt
      421 aaagttttgg gatttcaatt tttaagtgaa gtgggtggtg ctaatggttc actttctggt
      481 gtagctgcag ctatattggt tgctattgct attggtgtaa ccccaggata cgcagtttta
      541 attggtttat ctgtttctgg aacaggtatt attcctggtt tccttgcagg atatttagtg
      601 ggatttcttg ttaagtggat ggaaagaaat attcctggtg gtttagatct tatttcaatt
      661 attattattg gtgcaccatt aacacgtctt gtggccaaac tgttgacgcc gcttattaat
      721 agcactttgc tcaccattgg agatatctta acatcaggtg ctcatagcaa ccctatttta
      781 atggggatta tacttggcgg gacaattgta gtagtggcga cagcaccact ttcttctatg
      841 gcattgacag ctatgctagg attaaccgga atgcctatgg ctataggagc cttgtctgtc
      901 tttggttcgt catttatgaa tggtgtactt ttccataaat taaaacttgg aagtcgtaaa
      961 gataatatag cttttgctgt tgagcctcta actcaagctg acgtgacttc agctaaccct
     1021 attccaatct atgtcactaa ttttgttggt ggtgcagctt gtggtatttt aattgccttg
     1081 atgaaattag ttaatgatac tcctggaaca gcgacaccaa ttgcaggatt tgctgtcatg
     1141 tttgcctata acccaatgat aaaagtacta ataaccgctc taggttgtat tatcctatct
     1201 ttactagcag gctattttgg aggcattgtt tttaaagatt ataaattagt tacaaaagaa
     1261 g
//
LOCUS       DATRTN010000173         1228 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.175, whole genome shotgun sequence.
ACCESSION   DATRTN010000173 DATRTN010000000
VERSION     DATRTN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.175"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(275..358)
                     /locus_tag="VBE22_002013"
     tRNA            complement(275..358)
                     /locus_tag="VBE22_002013"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Leu,seq:caa)
     gene            complement(369..440)
                     /locus_tag="VBE22_002014"
     tRNA            complement(369..440)
                     /locus_tag="VBE22_002014"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Gln,seq:ttg)
     gene            complement(447..519)
                     /locus_tag="VBE22_002015"
     tRNA            complement(447..519)
                     /locus_tag="VBE22_002015"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(484..486),aa:His,seq:gtg)
     gene            complement(534..604)
                     /locus_tag="VBE22_002016"
     tRNA            complement(534..604)
                     /locus_tag="VBE22_002016"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Trp,seq:cca)
     gene            complement(611..691)
                     /locus_tag="VBE22_002017"
     tRNA            complement(611..691)
                     /locus_tag="VBE22_002017"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(655..657),aa:Tyr,seq:gta)
     gene            complement(703..775)
                     /locus_tag="VBE22_002018"
     tRNA            complement(703..775)
                     /locus_tag="VBE22_002018"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(740..742),aa:Phe,seq:gaa)
     gene            complement(778..851)
                     /locus_tag="VBE22_002019"
     tRNA            complement(778..851)
                     /locus_tag="VBE22_002019"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(815..817),aa:Met,seq:cat)
     gene            complement(862..951)
                     /locus_tag="VBE22_002020"
     tRNA            complement(862..951)
                     /locus_tag="VBE22_002020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(913..915),aa:Ser,seq:tga)
     gene            complement(965..1036)
                     /locus_tag="VBE22_002021"
     tRNA            complement(965..1036)
                     /locus_tag="VBE22_002021"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1001..1003),aa:Glu,seq:ttc)
     gene            complement(1047..1120)
                     /locus_tag="VBE22_002022"
     tRNA            complement(1047..1120)
                     /locus_tag="VBE22_002022"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1084..1086),aa:Ile,seq:gat)
     gene            complement(1157..1227)
                     /locus_tag="VBE22_002023"
     tRNA            complement(1157..1227)
                     /locus_tag="VBE22_002023"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1193..1195),aa:Gly,seq:tcc)
BASE COUNT          348 a          315 c          252 g          313 t
ORIGIN      
        1 aaaatcttat tattttatac tacctatcta tcattcacaa acacttttat tacttcagaa
       61 cctatgacat ttaggagtcc tctttgaatt tcatttaaaa tgttgagtct ccactaactc
      121 ttgaaaaatt tccttattat ttctgcttgt tttaaaactt caataacaat tgcaataatg
      181 aaaaacaaat ataagtaatt ttcagtaact ttttctcaaa attaccagta caatacaaaa
      241 aagacaaggt ctagaaacct tatcttttaa agtataccgg cggccggggt cgaaccggca
      301 cgtccgtgag gacactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcta
      361 tcttaaaact ggggtagctg gattcgaacc aacgcatgag ggagtcaaag tcccttgcct
      421 taccgcttgg ctatacccca tgaaaaggcg agtgatggga atcgaaccca cgaatgtcag
      481 agccacaatc tgatgtgtta accacttcac cacacccgcc atattagaaa aaacacgggc
      541 agtaggaatc gaacccacac tgaaggtttt ggagacctta gttctacctt taaactatgc
      601 ccgtttacta tggagagaga gggattcgaa cccccgaacc cgaaggagcg gatttacagt
      661 ccgccgcgtt tagcctcttc gctatctctc ctaaggtata aatggcgcga gacggaatcg
      721 aaccgccgac acatggagct tcaatccatt gctctaccaa ctgagctacc gagcctattg
      781 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct acctagctgc
      841 tccatcccgc gatatcttta aaggaggatg tgggattcga acccacgcac gcttttacac
      901 gcctgacggt tttcaagacc gttcccttca gccggacttg ggtaatcctc catataacaa
      961 aaaatagtcc gtacgggatt cgaacccgtg ttaccgccgt gaaaaggcgg tgtcttaacc
     1021 ccttgaccaa cggaccatat tcttgatggg cacgagtgga ctcgaaccac cgacctcacg
     1081 cttatcaggc gtgcgctcta accacctgag ctacgcgccc aagcaaatgc ttggttttta
     1141 ctttcttata aagtaaagcg ggtgacgaga atcgaactcg cgacaacagc ttggaaggct
     1201 gtagttttac cactaaacta cacccgct
//
LOCUS       DATRTN010000174         1204 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.176, whole genome shotgun sequence.
ACCESSION   DATRTN010000174 DATRTN010000000
VERSION     DATRTN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.176"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..907
                     /locus_tag="VBE22_002024"
     CDS             <1..907
                     /locus_tag="VBE22_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEO7203426.1"
                     /translation="ILESCTLSEGGHDFVRLYFNSPLEVKEISSYIFEEYINTKRSAT
                     EYLNYNKNYVDEYKASPLMENVLSNTDVKNILVSINNKVYNSQNNIIEKDLIDRKDIK
                     INPVNNRIPVSEIQNLTNWHKGFDLGFPFWEEGEKYRKEGNLKRSILLYDLARSNGYC
                     SPALFESYAMAFHRLKDYENEIDILDEGIEREGNLGRLITRRNNAMKMLIKSRNEQKK
                     IKENESAENILFSEIEILKRKKTRKNARPVLKMDDEMNILEHFESISDAVKKTGINSK
                     SIRDASKGIQKHAGGFVWRYEDEYK"
     gene            complement(1099..1204)
                     /locus_tag="VBE22_002025"
                     /pseudo
     CDS             complement(1099..1204)
                     /locus_tag="VBE22_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          485 a          132 c          205 g          382 t
ORIGIN      
        1 tattctagag tcttgtactt tatcagaagg tggtcatgat tttgttagac tatactttaa
       61 ttctccactt gaagtgaaag aaatttctag ttatattttt gaagaatata taaatactaa
      121 aagatctgca actgagtatt taaattataa taaaaactat gttgatgaat ataaggcaag
      181 tcctcttatg gaaaatgtac tttcaaacac tgatgtaaaa aatattttag ttagtataaa
      241 taataaagtt tataactcac aaaataatat tattgagaaa gatcttattg acagaaaaga
      301 tataaaaatt aatcctgtta ataatcgaat tcctgtttca gaaatacaaa atttgactaa
      361 ttggcataaa ggatttgatt tgggttttcc attctgggaa gaaggggaaa aatatagaaa
      421 agaaggaaat ttaaaacgat caattttgtt atatgattta gctagatcta atggctattg
      481 ttcgcccgca ctttttgagt catatgcaat ggcttttcat agattaaaag actatgaaaa
      541 tgaaattgat atcttagatg aaggaataga gcgtgagggc aatttaggta gattaattac
      601 acgtagaaat aatgcgatga aaatgttaat caaatcaaga aacgaacaaa agaaaataaa
      661 agaaaatgaa agtgctgaaa acatcctttt tagtgaaata gagattttaa aaagaaaaaa
      721 aactagaaaa aatgcacgtc cagtcttgaa aatggacgat gaaatgaata tattagaaca
      781 ttttgagtct atttcagatg cagtaaaaaa aacaggaatt aattcaaaaa gtatacgtga
      841 tgcttcaaaa ggtattcaaa agcatgcagg tggttttgtc tggagatacg aggacgaata
      901 taagtagatt cattcacata tataaaagtt ttgataagta attaatgatt tcataataat
      961 cgtattgaat gaaataattt actaaagtag acaagacaaa ataataatat cagtgtttat
     1021 ttttagtcat tattatagca ttggtaaaaa cctgaaaagg caacacttta taaatgttgt
     1081 acagaaaagt gttgcctttt cagtcttaag agctagttct tccagcgatt gaaaggtttc
     1141 ttgataaaca aattcaattt tgaaagcacg atacgtactt tcagctacgg cattgtcata
     1201 agga
//
LOCUS       DATRTN010000175         1149 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.177, whole genome shotgun sequence.
ACCESSION   DATRTN010000175 DATRTN010000000
VERSION     DATRTN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.177"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..111)
                     /locus_tag="VBE22_002026"
     CDS             complement(<1..111)
                     /locus_tag="VBE22_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEO7203427.1"
                     /translation="MQQVFNVSVPVPDDVVIISKEEYLNLLSDNEQGKWWD"
     gene            complement(304..936)
                     /locus_tag="VBE22_002027"
     CDS             complement(304..936)
                     /locus_tag="VBE22_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203428.1"
                     /translation="MIGDYSVMDWATLGGVVGIIFTNVGVLISNSRDNKAIQRELELL
                     EEGQEKLVDEFSKISTNQYDKYVLIQSNLSNNIEKNKQELVQKNSYVKEDTKYIRDEM
                     LIEKKSREEVYNHVKNGDKLIEKMAFANELILKFGEVSRENQMLGLKVNSLEEKIVDL
                     SNQPKNDEISKLRKSISSFERELSRFEDGGYSEAEEIKSTLRRILNDLEG"
BASE COUNT          385 a          229 c           88 g          447 t
ORIGIN      
        1 atcccaccac ttgccttgtt cattatcact taacaaattt aaatattctt cttttgaaat
       61 aataacaaca tcatctggta caggaacact cacattaaaa acctgttgca tactcatcct
      121 ccttataact tgcttaatcc gaacgttatt tgttccaggt aattaggttt tccactttaa
      181 aaccgaacta tttttcaatt gcagttttaa tttaaaatcc atatcccccg aacattacgc
      241 tttaataaaa aaacaaacaa aaaaagccat gaaattaatc acaactttat ttctatcaac
      301 ttcttatcct tcaagatcat ttaatattct tcttaaagta ctttttattt cttctgcttc
      361 gctataaccg ccatcctcaa acctagataa ttccctttca aaacttgaaa ttgatttacg
      421 taattttgat atttcatcat ttttaggttg atttgataaa tcgactattt tttcctctaa
      481 agaattaact tttaagccta acatttgatt ttctctgctt acttcaccaa acttcaaaat
      541 taattcatta gcaaaagcca ttttttcaat aagtttatct ccatttttca catgattata
      601 aacttcttcc cttgactttt tctcaataag catttcatca cggatatatt tagtatcttc
      661 tttaacatat gagttttttt gaactaattc ttgtttattc ttttcaatat tgtttgataa
      721 attgctttgt atcagaacat atttatcata ttgatttgtc gaaattttgg aaaattcgtc
      781 taccaatttc tcttgacctt cttctaaaag ctcaagttct cgttgaatag ctttattatc
      841 cctagagttt gaaatcaaaa ctcccacatt tgtaaaaata attcctacaa caccacctaa
      901 agtagcccaa tccattactg aataatcccc tatcattttt tctcctattc tatcacattt
      961 tattccattc tgtattattt aattaaaaaa gccatgaaac taatcatgac atcaatgttt
     1021 ctccatatct aataaaatat catttatcaa aaataacaaa tactttctaa cttctctttt
     1081 tttagaatca tgcaaaaaga attctttaaa acctacataa taaatatata aaacaaatat
     1141 ccatacaat
//
LOCUS       DATRTN010000176         1098 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.178, whole genome shotgun sequence.
ACCESSION   DATRTN010000176 DATRTN010000000
VERSION     DATRTN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.178"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(33..>1098)
                     /gene="dnaN"
                     /locus_tag="VBE22_002028"
     CDS             complement(33..>1098)
                     /gene="dnaN"
                     /locus_tag="VBE22_002028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEO7203429.1"
                     /translation="KNAIPILSTVKIEVTRDAIILTGSNGQISIENTIPASNENAGLL
                     VTNPGSILLEAGFFINIISSLPDVTLEFTEIEQHQIVLTSGKSEITLKGKDVDQYPRL
                     QEMTTDTPLTLETKLLKSIINETAFAASQQESRPILTGVHLVISQNKYFKAVATDSHR
                     MSQRTFQLEKSANNFDLVVPSKSLREFSAVFTDDIETVEVFFSDSQMLFRSENISFYT
                     RLLEGNYPDTDRLLTNQFETEIIFNTNALRHAMERAYLISNATQNGTVRLEIQNETVS
                     AHVNSPEVGKVNEELDTVSLKGDSLNISFNPTYLIESLKAVKSETVTIRFISPVRPFT
                     LTPGEDTEDFIQLITPVRTN"
BASE COUNT          356 a          184 c          188 g          370 t
ORIGIN      
        1 ctacccaaga ataacttggg tagttttctt atttagttag tacgaacagg agttattaat
       61 tggatgaaat cttcagtatc ttcaccaggt gtcaaagtaa atggacgtac tggagaaata
      121 aatcgaatcg taactgtttc gctttttact gcttttaaag attcaattag gtaagttgga
      181 ttaaagctaa tatttaaact atcaccttta agactaacag tatccaattc ctcattaact
      241 ttaccaactt ctggagagtt tacatgagct gagactgtct cattttgaat ttctaaacga
      301 acagtaccgt tctgagttgc attcgaaatt aaataagcac gttccatagc atggcgtaaa
      361 gcatttgtat taaagataat ttcggtttca aactgattag ttaggaggcg atcagtatca
      421 gggtagtttc cttcaagcaa acgtgtatag aagctgatat tttcacttct aaataacatt
      481 tgactatctg agaagaaaac ctctacagtt tcaatatcat ctgtaaaaac agccgaaaat
      541 tctcgaaggg atttacttgg aacaaccaaa tcaaaattat tagccgattt ctctaattgg
      601 aaagtgcgtt gactcatacg gtgtgaatct gtcgcaacag ccttaaagta tttattttga
      661 ctgataacca aatgaacacc tgttaagatt ggacggcttt cttgttggct agcagcaaaa
      721 gcagtttcat taataattga ttttaacagt ttagtttcta atgttaatgg agtatctgtt
      781 gtcatttcct gcagacgagg gtattgatcg acatccttac ctttcaaagt aatctctgat
      841 tttccactag taagaacaat ttgatgttgt tcaatctctg taaattctaa agttacatct
      901 ggtaaacttg aaataatatt aataaagaaa ccagcttcta acaaaataga gcctggattc
      961 gttactagta aacctgcatt ttcatttgaa gcaggaatag tattttcaat tgaaatttgt
     1021 ccatttgacc ccgttaaaat gatagcatcc ctagtcactt ctattttaac agttgaaagg
     1081 attggaatcg cattttta
//
LOCUS       DATRTN010000177         1058 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.181, whole genome shotgun sequence.
ACCESSION   DATRTN010000177 DATRTN010000000
VERSION     DATRTN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.181"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            74..772
                     /locus_tag="VBE22_002029"
     CDS             74..772
                     /locus_tag="VBE22_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEO7203430.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAITSVR
                     EGEGKSTTSTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIISNACDGFILVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVSESVATYGDY
                     GDYGNYGKRDRKRK"
     gene            785..>1058
                     /locus_tag="VBE22_002030"
     CDS             785..>1058
                     /locus_tag="VBE22_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203431.1"
                     /translation="MKEKENIQKIIIAMIQTVVVYFSASLTLTLITPNFKSNKDLLFV
                     LLIHYIVFYLSDFYRDFWSRGYLEEFKMVLKYSFYYIFISSSLFFIF"
BASE COUNT          372 a          142 c          191 g          353 t
ORIGIN      
        1 gatattgaaa aggccctagg acttacttct ttaggtatag taccagattt gaataaactt
       61 taaggagaat ataatgactc gtttagaaat agttgatagc aagttaagac aagcaaaaaa
      121 aacagaagaa tacttcaatg cgatccgtac aaacatacag tttagtggaa aggaaaataa
      181 aattcttgca attacctctg ttagggaagg ggaaggaaaa tccactactt caacaagttt
      241 agctttatct ttagctcaag caggatttaa aacattatta attgatgcgg atactaggaa
      301 ctctgttatg tctggaacct ttaaagcaac tggaactatt aaaggcttga cgaattattt
      361 atcaggtaat gcagatcttg gagatattat ctgtgaaacc aatgttccta gactgatggt
      421 cgttccttca gggaaagtac caccaaatcc aacagcatta cttcagaacg cttattttaa
      481 taagatgatt gaagctatta aaaatatatt tgattatatt atcatcgata ctccacctat
      541 tggtttagtt gttgatgccg caataatctc taatgcttgc gatggtttta ttttagtaac
      601 ccaagcaggt agaataaaac gtaattatgt tgaaaaagca aaagaacaga tggaacaaag
      661 tggttcaaag ttcttaggta ttattcttaa taaagttagt gaatctgttg ctacttacgg
      721 cgattacggc gattatggaa attacggaaa aagggataga aaaaggaagt aaggggctct
      781 tgtattgaaa gaaaaagaaa atatacaaaa gattattata gcgatgattc aaacagttgt
      841 ggtttatttt tctgcaagtt tgacattaac attaattact cccaatttta aaagcaataa
      901 agatttattg tttgttctat tgatacatta tattgtcttt tatctttctg atttttacag
      961 agacttttgg agtcgtggct atcttgaaga gtttaaaatg gtattgaaat acagctttta
     1021 ctatattttc atatcaagtt cattattttt tattttta
//
LOCUS       DATRTN010000178         1057 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.182, whole genome shotgun sequence.
ACCESSION   DATRTN010000178 DATRTN010000000
VERSION     DATRTN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.182"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..>1057
                     /locus_tag="VBE22_002031"
     CDS             <1..>1057
                     /locus_tag="VBE22_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO7203432.1"
                     /translation="LLLKSLHSLLGLTITVCDQNFSVIREYKSEKTISLFYNHYLILS
                     NFSKTQHDFLFHYGSLGELFLVHHIQQYYIIIGPWRSNVIDPLLLKKKLTETQINASE
                     QNYFIDRLSQLPFFSLSQIRELLIVTNYCLTGVVKDKLSEPLHYYTKGWSNSFDLDKI
                     KQFSKQNMSSYKYQYHFENNILKAVKSGSEFLLKETVEQFSNSIVPIISGDELRSEKN
                     YSIMIYDRLSQATIQAGLDIETAYRARDRFIKENESTISLNEVLKLRDTAILFYTQQV
                     HSLKKHLETPHSQTIVAVIRYLENNLNRFIKTEEIAKECHMSESKLRKLFKQEKHITI
                     QQYFLNLKIEAAKQLLD"
BASE COUNT          382 a          188 c          143 g          344 t
ORIGIN      
        1 ttctactgtt gaaatcatta cacagtcttc taggtctaac tattactgtt tgtgatcaaa
       61 atttttctgt tatcagagag tataaatccg agaaaactat ttcacttttt tacaatcatt
      121 accttatttt aagtaacttt agtaaaactc aacatgattt tttatttcat tatggctctt
      181 taggagaact ttttcttgtc caccatattc aacaatatta tattatcatc ggtccctggc
      241 gtagcaatgt tattgaccct ttacttctta agaaaaagct gactgaaaca caaatcaatg
      301 ctagtgaaca aaactacttt attgatagac tatcccaatt acctttcttc tcacttagtc
      361 aaatccgaga attactaata gtaaccaatt attgtctaac tggggttgtg aaagataaat
      421 tatcagagcc cttacattac tacacgaaag ggtggagcaa ctcctttgat ctagataaaa
      481 ttaagcaatt ttctaaacag aatatgagtt cttacaaata tcagtatcat tttgaaaata
      541 atattctcaa agctgttaaa tcaggtagtg aatttctttt gaaagaaaca gtggaacaat
      601 ttagcaattc tatcgtacct ataatcagtg gagatgaatt gcgatctgaa aaaaactact
      661 ccatcatgat ttatgatcgt ctctcacagg ctaccatcca agcaggactt gatatcgaaa
      721 cagcttatcg agcacgagat cgttttataa aagaaaacga gtcaactata agtctaaatg
      781 aagttttaaa attacgtgat actgctatct tattctatac tcaacaagtt cattctttaa
      841 aaaaacatct cgaaacccct cattctcaaa ctattgtcgc agtgattcga tatcttgaaa
      901 ataatctaaa tcgctttatt aaaacagaag aaatagctaa agaatgtcac atgagtgaat
      961 cgaagttacg aaaactattt aagcaagaaa aacacatcac tattcaacaa tattttctaa
     1021 acttaaaaat cgaagctgct aagcaattac tagatga
//
LOCUS       DATRTN010000179         1039 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.183, whole genome shotgun sequence.
ACCESSION   DATRTN010000179 DATRTN010000000
VERSION     DATRTN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.183"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..237)
                     /locus_tag="VBE22_002032"
     CDS             complement(<1..237)
                     /locus_tag="VBE22_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7203433.1"
                     /translation="MKKLFNKRRSLFLTQNSKYLRYVFNDHFVLVLMFLSGFLLYQYS
                     QLLKDFPKTHWPIIVIVSIIILMLLAMGGIASYLE"
     gene            complement(239..964)
                     /locus_tag="VBE22_002033"
     CDS             complement(239..964)
                     /locus_tag="VBE22_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7203434.1"
                     /translation="MLKIENVTGGYVNIPVLKNISFEVNDGELVGLIGLNGAGKSTTI
                     NEIIGILRPYQGDITIDGISLEANQELYRKKIGFIPETPSLYEELTLREHLETVAMAY
                     DIATDEVMARAQKLLEMFRLTDKLDWFPMHFSKGMKQKVMIICAFVVSPSLFIVDEPF
                     LGLDPLAISDLINLLAEEKAKGKSILMSTHVLDSAEKMCDRFVILHKGEIRAVGTLEE
                     LRAIFGDSNANLNDIYIALTKEV"
BASE COUNT          348 a          200 c          139 g          352 t
ORIGIN      
        1 ttctaaatag ctggctattc cccccatcgc tagtaacatt aagataatga ttgacacaat
       61 cacgataatt ggccaatgtg tttttggaaa gtcttttagt aattggctat actgatataa
      121 caaaaaacct gacaaaaaca ttaatactaa tacaaaatga tcattaaaga cgtatctcaa
      181 atatttacta ttttgagtta aaaagagact tcgacgttta ttaaataact ttttcatatc
      241 acacctcttt tgtcagagct atgtagatgt catttaaatt agcattactg tctccaaaaa
      301 tagcccgtaa ttcctctagg gttccaactg cccgtatttc tcctttatgt aaaataacaa
      361 aacgatcaca cattttttcg gcagaatcta atacgtgtgt tgacatcaaa atagatttcc
      421 ctttagcttt ttcttctgct agaagattaa tcaaatctga aatagctagt ggatccaaac
      481 ctaaaaatgg ttcatcaaca ataaataaac ttggagatac aacaaaagca caaataatca
      541 taaccttttg tttcattccc tttgaaaaat gcattggaaa ccagtctaat ttatctgtta
      601 gacgaaacat ctctaatagt ttttgcgcac gtgccataac ttcatcggtg gcaatatcat
      661 acgccatagc taccgtttct aaatgttccc tgagagttag ttcctcatag agacttggtg
      721 tctcaggaat aaagcctatt ttctttctat aaagttcttg gttggcttca agactaatgc
      781 catcaatggt aatatctcct tgatagggtc tcaaaatacc aataatctcg ttaatagtgg
      841 ttgattttcc tgcaccatta agcccaatta atccaactaa ctctccatca tttacttcaa
      901 aagaaatatt ttttaaaact gggatattaa catacccccc tgtaacattt tcaattttta
      961 acattgtcat tctctctatc tttgatatac tttattaact attataacaa aataagaaag
     1021 ggaaggtcta ttcttatgg
//
LOCUS       DATRTN010000180         1037 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.184, whole genome shotgun sequence.
ACCESSION   DATRTN010000180 DATRTN010000000
VERSION     DATRTN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.184"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..235
                     /locus_tag="VBE22_002034"
     CDS             <1..235
                     /locus_tag="VBE22_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203435.1"
                     /translation="SIIGMLLAGCQMNSESKSQSNEVKNSKHSAVKKDKKMTKKEQLV
                     YLNEHEQEIIDFVKSHNQQIESVQFDWSGIKVE"
     gene            491..811
                     /locus_tag="VBE22_002035"
     CDS             491..811
                     /locus_tag="VBE22_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203436.1"
                     /translation="MSNWNLDNFDNLHSSLAESAYNSRPNSFPELFETDSVTEVKFSQ
                     PSEDNKGQITQGGTNLPNDGIVYLQPDKSLKSIDENVKVRVPDVNGGYHSEHCVTNSY
                     QKGY"
BASE COUNT          393 a          149 c          166 g          329 t
ORIGIN      
        1 atctattatt gggatgttac tagcaggctg tcaaatgaat agtgaatcta aaagtcagtc
       61 aaacgaagta aaaaatagca aacactcagc agtaaagaaa gataaaaaaa tgacaaaaaa
      121 agaacaatta gtttatctca atgagcatga acaagaaata attgattttg taaaatcaca
      181 taaccaacaa attgagtccg ttcaattcga ttggtcaggt ataaaagtag aataaagtgg
      241 aaatggaact ccacaagggg gtgattataa tctttcactg agaggaaagt ttaatcatct
      301 acaaaattca aaattaatag ttgattttta tttagctcat aaaaatgata tcccaaatat
      361 caaatcaatg ggaatgctaa ataagccgta tatacataaa aatggtattt ggcacattta
      421 tgaatagctt gaatcatcct atataaagta atagaatttt taccacaagc aaggtagagg
      481 agaagaataa ttgagtaatt ggaacttaga taattttgat aacttacata gctctttagc
      541 agaaagtgca tataatagtc gacctaattc atttcctgaa ttatttgaga ctgactcagt
      601 aacagaagta aaattttctc aaccctcaga agataacaaa ggtcaaatca cacagggtgg
      661 cactaacctc cccaatgatg gtattgtcta cctgcaacca gataagtcct taaaatctat
      721 tgatgaaaat gtaaaggttc gcgttcctga tgtcaatggc ggttatcact ctgagcattg
      781 tgttactaat tcctatcaga aaggttactg aaagatgatg aagcagtaac tctataatca
      841 ctaaaagctc atcattgctt agtgtttaag ttaacttcta aaactcaaac cttattaaat
      901 caagatttgt ttacttttaa aagtgacatt ttatttttct aaaaatgaga ttattgggat
      961 tcctcattat aagttacttt gtttatatgt acctttactt aagggttatt ttcattattt
     1021 ttaatttaat attttgg
//
LOCUS       DATRTN010000181         1013 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.186, whole genome shotgun sequence.
ACCESSION   DATRTN010000181 DATRTN010000000
VERSION     DATRTN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.186"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..322
                     /gene="tilS"
                     /locus_tag="VBE22_002036"
     CDS             <1..322
                     /gene="tilS"
                     /locus_tag="VBE22_002036"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO7203437.1"
                     /translation="IFSQYLFSYGKFISQADITIPIYDTSPIILRRRKEGDRIFLGNH
                     TKKIRRLFIDEKITLKEREEAVIGEQNKELIFVIVAGRTYLRKPSEHDIMKGKLYIEN
                     LEKR"
     gene            327..869
                     /gene="hpt"
                     /locus_tag="VBE22_002037"
     CDS             327..869
                     /gene="hpt"
                     /locus_tag="VBE22_002037"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEO7203438.1"
                     /translation="MLENDIKKVLYSEEDIILKTKELGAKLTADYAGKNPLLVGVLKG
                     SVPFMAELLKHIDTHVEIDFMVVSSYHGGTTSSGEVKILKDVDTNIEGRDVIFIEDII
                     DTGRTLKYLRDMFKYRQANSVKVATLFDKPEGRLVDIDADYVCYDIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEIYSK"
BASE COUNT          377 a          123 c          183 g          330 t
ORIGIN      
        1 aatattctca caatatttat tttcctatgg aaaattcatt tctcaagcag acattactat
       61 tcctatttat gatacttctc cgattatttt aagaagaaga aaagaagggg ataggatatt
      121 tttaggaaat cacactaaaa aaattcgccg tctatttatt gatgagaaaa taactctaaa
      181 agagagagaa gaagctgtta ttggggagca aaataaagag ttgatatttg tgattgttgc
      241 aggtagaaca tatttgagaa aaccttctga acatgatata atgaagggca aactttatat
      301 tgaaaattta gaaaaaaggt gacatcatgc ttgaaaatga tataaaaaaa gttttatatt
      361 cagaagaaga tattattctg aaaacgaaag aattaggagc taaattaact gcagattatg
      421 caggtaagaa tccattatta gtaggtgtat taaaggggtc agtgcctttt atggctgagc
      481 tattaaagca tattgataca catgttgaaa ttgattttat ggttgtttca agctatcatg
      541 gtggtacaac tagtagtgga gaagttaaaa ttttaaaaga tgttgatact aacattgaag
      601 gaagagatgt aatctttatt gaagacatca ttgatactgg acgtacatta aaataccttc
      661 gagatatgtt taagtatcgc caagctaact ctgtcaaagt tgcaaccctt tttgataagc
      721 cagaagggcg attggtagat attgatgctg attacgtttg ttacgatatc ccaaatgaat
      781 ttattgtggg attcggttta gactatgctg aaaactatcg taacttacct tatgttggtg
      841 tgttaaaaga agaaatttac tcaaaataga aaaaaggtac tctcttaaac tatgaaaaat
      901 aataaaaata atggttttct gaaaaattcc tttatttaca tattattgat tattgcggtt
      961 attacaacct ttcaatacta tttaaaagga actagctctc aaaaccagca aat
//
LOCUS       DATRTN010000182          998 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.187, whole genome shotgun sequence.
ACCESSION   DATRTN010000182 DATRTN010000000
VERSION     DATRTN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.187"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..100
                     /locus_tag="VBE22_002038"
     CDS             <1..100
                     /locus_tag="VBE22_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7203439.1"
                     /translation="THSKEVADSADIILELSGKKLKKVNKMNLEVE"
     gene            100..>998
                     /locus_tag="VBE22_002039"
     CDS             100..>998
                     /locus_tag="VBE22_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO7203440.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMATLSIISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTNSIDSYVKRINSVADLV
                     DHDIIETQDTLANQSPERAKNFKRTVMLTGVNDSAKETKFVSEAYKLVEGKHLENKDK
                     NKILMHKDLAKKNNLKVGDKIKIKSNLFDADNEKVANETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHSAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSIS"
BASE COUNT          426 a          120 c          176 g          276 t
ORIGIN      
        1 aacacatagt aaggaagtag cagattctgc ggatatcatt ttagaactaa gtggtaaaaa
       61 gttgaaaaaa gtaaataaaa tgaatttgga ggttgaataa tgataaaaaa tgcatttgct
      121 tatgtaacta gaaaaagctt aaaatcatta attattatat tagttattct atcaatggca
      181 actttaagca tcattagttt atctattaaa gatgccacag atagagcttc taaggaaaca
      241 tttgctaata taacaaacag tttttctatg gaaataaata gacaagtaaa tccaggaaca
      301 cctaggggtg gaggaaatgt aaaaggtgaa gatattaaaa agatatctca aacaaacagt
      361 atagactcct atgtaaaaag aataaacagc gtcgcagacc tagttgatca tgatataatt
      421 gaaactcaag atacacttgc aaatcaatca cctgaaagag caaagaattt taagagaaca
      481 gttatgttaa caggggttaa tgactcagca aaagaaacaa aatttgtatc agaagcatat
      541 aagttagtag aaggaaagca tttagaaaat aaagataaaa ataaaatttt aatgcataaa
      601 gatttggcaa aaaaaaacaa tctcaaagtt ggagataaaa taaaaataaa atctaatttg
      661 tttgatgctg acaatgaaaa agttgcaaat gaaactgtag aagtagaaat taaaggttta
      721 tttgatggtc ataatagcgg tggggtaagt gcagcacaag aactatacga aaatacacta
      781 atcactgacg ttcattcagc tgctaaagtt tatggtaata cagaagatac agccgtatac
      841 caagatgcaa cattctttgt aaaaggtgat aagaatcttg atagtgtaat aaaagatctt
      901 gggaaactag atataaattg gagagaatat aatttgatta aaagttcatc aaattatcct
      961 gcgctgcaac aatctatatc cggtatctat tctatttc
//
LOCUS       DATRTN010000183          989 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.188, whole genome shotgun sequence.
ACCESSION   DATRTN010000183 DATRTN010000000
VERSION     DATRTN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.188"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            23..838
                     /locus_tag="VBE22_002040"
     CDS             23..838
                     /locus_tag="VBE22_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO7203441.1"
                     /translation="MIYGFVGPNGAGKTTTIKMILGLLKFDTGFITIFGKKVNFGRTD
                     TNQLIGYLPDVPEYYDYMTALEYLDLCSGLARSKHKLSNKELLRSVGLDDNHQKIATY
                     SRGMKQRLGLAQALVHDPKIIICDEPTSALDPKGRQDILDIISNLRGEKTVIFSTHIL
                     SDVEKICDHVLVLTKCGIYSLEELKGKKSEENYSVRILIKVTKSEAKVLSHNYQIEKK
                     DNEYALTLKGSKMDNKADLLAGFYQDLVSLKISPSAIEVIDNSLEELYLEVTK"
BASE COUNT          344 a          143 c          184 g          318 t
ORIGIN      
        1 ttcttttgat ttagaagaaa atatgattta tggattcgta ggaccaaatg gagcaggtaa
       61 aacaacgaca ataaaaatga ttttaggatt gttaaaattt gatacgggtt tcataacaat
      121 ttttggtaaa aaagttaatt ttggaagaac agataccaat caactgattg gctatctccc
      181 agatgttccc gagtactacg attatatgac agctctagaa tatcttgatt tatgttctgg
      241 tttagcgaga tcaaagcata agctttctaa caaagaacta cttaggtcag ttggacttga
      301 cgataatcac caaaagattg ctacttattc tcgaggaatg aaacagcgtc ttggattagc
      361 ccaagcactg gttcatgatc ctaaaattat tatttgcgat gaaccaacct ctgcattaga
      421 cccgaaaggt cgccaagata ttttagatat tatttcaaat cttagaggag aaaaaactgt
      481 gattttttct acccatattc tctctgatgt cgaaaaaata tgtgatcatg ttctggtgtt
      541 aactaagtgt gggatttatt ctcttgaaga gttaaaagga aaaaaatcag aggaaaatta
      601 ttcggttcgt attttaataa aagtgacaaa gtctgaagct aaggtactat ctcataacta
      661 tcagatagaa aagaaagata atgagtatgc actgactctc aaaggctcta aaatggataa
      721 caaagcagat ttactagctg gtttttacca agatttagtt tctttaaaga ttagcccatc
      781 agctattgaa gtcattgaca atagtttaga ggagctttat ttggaggtta ccaaatgaaa
      841 tctttagcag taatgttaaa aaaagaatgg atggagaatg ttaggacata caaagttata
      901 tctattctga ttacttgttc aatatttggt atattaggtc ccttaactgc acttatgatg
      961 cctgatatta tggcaggaat tttacctaa
//
LOCUS       DATRTN010000184          984 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.189, whole genome shotgun sequence.
ACCESSION   DATRTN010000184 DATRTN010000000
VERSION     DATRTN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.189"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            161..>984
                     /locus_tag="VBE22_002041"
     CDS             161..>984
                     /locus_tag="VBE22_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203442.1"
                     /translation="MNKKHKLLKNIEEFKTITQKRLTERGKFPYDTVHSTFEIKDENF
                     IMERLKSSGLSMGKPVDYMGVNGIPIYTKTLSIDNKFAFENNSKDSSYSSNINISEDK
                     IKENDQKILDLIVKSGANNQNLTDEEKVIAFTKYIGEITNYDNEAYRARNVDTEYYRA
                     SDLFSVTERKLAMCVGYSVTAARAFNIMGIPSYVVSGKSPQGISHAAVRAYYNRSWHI
                     IDITASTYWKNGNYKTTYSDFIKEYCIDGYDVYDPAKTNNRFKVKYMESNEAFENW"
BASE COUNT          400 a          123 c          166 g          295 t
ORIGIN      
        1 ttttaatgtt tggattttag gtgataaagg ctattacaaa aaccttatca atttagaaga
       61 aattaaaaat atccaagcta cattaaaaaa agaaagaaat gaagaatatg tctttgtcaa
      121 attaaatggg aaaatcgcac atgatacaac cgtattcttg atgaataaaa aacataaatt
      181 acttaaaaat atagaggaat ttaagacgat aacccaaaaa aggctaactg agagaggaaa
      241 gttcccatat gataccgttc attctacgtt tgaaattaag gatgaaaact ttatcatgga
      301 aagattgaaa tcatcagggt tatctatggg taaaccagtt gattatatgg gcgtaaatgg
      361 tatccctatt tacactaaaa cattatctat agataataag tttgcctttg aaaataattc
      421 aaaagattct tcatattctt caaacattaa tattagtgaa gataaaataa aagaaaatga
      481 ccaaaaaatt ctagatttaa tagttaaatc tggagctaat aatcaaaact taacagatga
      541 agaaaaagta attgctttca ctaaatatat aggtgaaata actaattatg acaatgaagc
      601 ttacagagct agaaatgtcg acacagaata ttacagagct tcagatttat tttctgtaac
      661 agaaagaaaa cttgctatgt gcgttggcta tagtgtaaca gccgctaggg catttaatat
      721 tatggggata cctagttatg tcgtttctgg taaaagtcct caaggaatat ctcatgcagc
      781 tgtgagagcg tattataata gaagttggca tatcatagat atcacagcca gtacatattg
      841 gaaaaatggt aattacaaaa caacgtattc cgattttatt aaagaatact gtatagatgg
      901 ttatgacgtt tatgatcccg caaaaacaaa taatagattt aaagttaagt atatggaatc
      961 aaatgaagcg tttgaaaatt ggat
//
LOCUS       DATRTN010000185          983 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.190, whole genome shotgun sequence.
ACCESSION   DATRTN010000185 DATRTN010000000
VERSION     DATRTN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.190"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..924)
                     /gene="tilS"
                     /locus_tag="VBE22_002042"
     CDS             complement(<1..924)
                     /gene="tilS"
                     /locus_tag="VBE22_002042"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO7203443.1"
                     /translation="MYNTILKDTLSKGLFTAHQKVLIAVSGGIDSINLLQFLYQYQKE
                     LSISIGIAHINHGQRKESEKEEEYIRQWGQIHDVPVFISYFQGIFSEDRARNHRYNFF
                     SKVMREEGYTALVTAHHADDQAETVFMRILRGSRLRYLSGIKQVSAFANGQLIRPFLP
                     YKKELLPNIFHFEDASNASSDYLRNRIRNVYFPALERENNQLKDSLITLSEETECLFT
                     ALTDLTRSIEVTNCYDFLRQTHSVQEFLLQDYISKFPDLQVSKEQFRVILKLIRTKAN
                     IDYTIKSGYFLHKDYESFHITKIHPKTDSFKV"
BASE COUNT          314 a          171 c          154 g          344 t
ORIGIN      
        1 aaccttaaaa ctatccgtct taggatggat ttttgtaata tgaaaactct cgtaatcctt
       61 atgaagaaaa tagcccgatt taatagtata gtcaatattg gctttagttc taatcaattt
      121 taatattact ctgaactgtt ctttactcac ttgtaaatct ggaaatttgc taatgtaatc
      181 ttgcaataaa aattcttgga cagaatgagt ttgccttaaa aaatcatagc agtttgttac
      241 ttcgatgctc cttgttaggt ctgttaaagc cgtgaataaa cactctgttt cctcagagag
      301 agttatgaga gaatctttca attgattatt ttctctttcc aaagcaggaa agtagacgtt
      361 tcttattcga tttcttaaat aatcagacga agcgttgcta gcatcttcaa aatgaaaaat
      421 atttggtaat aactcctttt tatatggaag aaatggtcta atcaattgac cgtttgcaaa
      481 agcactcact tgcttaatcc ctgataaata gcgtagcctg cttcctctta aaattctcat
      541 aaatacagtt tcagcttgat catcagcatg gtgagctgtt actaaagccg tgtaaccttc
      601 ttcacgcata acttttgaaa aaaaattgta gcgatggtta cgtgctctat cttctgaaaa
      661 tattccttga aaataagaga taaagacagg gacatcatgt atctgtcccc actgtctgat
      721 atattcttct tctttttctg attcttttcg ctgtccatga tttatgtgtg caataccaat
      781 actaatcgat aactcttttt gatactggta taaaaattgc aacaaattta ttgaatcaat
      841 gccaccagaa acagcaatta gaactttctg atgagcggta aataaccctt tggataaggt
      901 atctttcaaa atagtgttat acatattatt ttaaaacctg gtaaacgtcg tcagcaatct
      961 tagtgatatc atcgtaagat gat
//
LOCUS       DATRTN010000186          899 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.192, whole genome shotgun sequence.
ACCESSION   DATRTN010000186 DATRTN010000000
VERSION     DATRTN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.192"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            38..526
                     /locus_tag="VBE22_002043"
     CDS             38..526
                     /locus_tag="VBE22_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEO7203444.1"
                     /translation="MITLLKDQSFDEISTINLTKTAGISRSSFYTHYKDKYEMIDQYQ
                     QSLFNKVEYIFDRNQFKKEDALLEIFQFLDRESLFAALLTQNGTKEIQTYILNKLQLM
                     LSKELPVVNPDATKSDINRLYYSVYLSHAIFGVYQMWITRGKKESPQQITQVLLSLLP
                     QT"
     gene            complement(612..>899)
                     /locus_tag="VBE22_002044"
     CDS             complement(612..>899)
                     /locus_tag="VBE22_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEO7203445.1"
                     /translation="NDRLKIEEMEEKYDSFKPRINALVEAIDDFQKHYEDYVKLREFY
                     GSEDWFRLSEQTENNLKCGVLSEDQLFDFIGEHNELVGQFLDMSSQMYRHL"
BASE COUNT          313 a          163 c          123 g          300 t
ORIGIN      
        1 gaggagagaa aaaacaaaaa gagctattga agcagctatg attacgttat taaaagatca
       61 gagcttcgat gaaatttcaa cgattaatct aactaaaaca gctggaatca gtcgttctag
      121 tttttatact cattataaag ataaatatga aatgattgat caataccaac agtcactctt
      181 caataaagtc gaatatattt ttgatcgcaa tcaatttaaa aaagaagatg ccttgctcga
      241 aatttttcaa ttcttagata gagaatcatt atttgctgct ctcttaaccc aaaatggaac
      301 aaaagaaatt cagacctaca tccttaataa attacaatta atgctttcta aagaattacc
      361 tgtggtcaat ccagatgcaa caaagtctga tatcaaccgt ttgtattata gtgtctatct
      421 cagtcatgct atttttggag tctatcaaat gtggataaca cgaggaaaaa aagaaagccc
      481 tcaacaaatt acacaagtgc tactcagttt attacctcaa acttaaaacc agccttcctt
      541 ataggctggt catttttagt taggtttatt ttatcctcat tataggataa caacactaat
      601 aaggactgac attataaatg acgatacatt tgagaagaca tatctaaaaa ttggcctaca
      661 agctcgttat gttctcctat aaaatcaaag agctgatcct cactcagtac accgcatttg
      721 agattatttt ctgtttgttc tgatagtcta aaccagtctt cgctaccata aaattctctt
      781 aatttcacgt aatcttcata gtgcttttga aaatcatcta tagcttctac taatgcattg
      841 attctaggtt tgaagctatc atacttctct tccatttctt ctatttttaa tctatcatt
//
LOCUS       DATRTN010000187          865 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.193, whole genome shotgun sequence.
ACCESSION   DATRTN010000187 DATRTN010000000
VERSION     DATRTN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.193"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(1..73)
                     /locus_tag="VBE22_002045"
     tRNA            complement(1..73)
                     /locus_tag="VBE22_002045"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37..39),aa:Ile,seq:cat)
     gene            complement(93..166)
                     /locus_tag="VBE22_002046"
     tRNA            complement(93..166)
                     /locus_tag="VBE22_002046"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Met,seq:cat)
     gene            complement(182..255)
                     /locus_tag="VBE22_002047"
     tRNA            complement(182..255)
                     /locus_tag="VBE22_002047"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(219..221),aa:Pro,seq:tgg)
     gene            complement(263..336)
                     /locus_tag="VBE22_002048"
     tRNA            complement(263..336)
                     /locus_tag="VBE22_002048"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(348..432)
                     /locus_tag="VBE22_002049"
     tRNA            complement(348..432)
                     /locus_tag="VBE22_002049"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(396..398),aa:Leu,seq:taa)
     gene            complement(441..512)
                     /locus_tag="VBE22_002050"
     tRNA            complement(441..512)
                     /locus_tag="VBE22_002050"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Gly,seq:gcc)
     gene            complement(546..618)
                     /locus_tag="VBE22_002051"
     tRNA            complement(546..618)
                     /locus_tag="VBE22_002051"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(583..585),aa:Thr,seq:tgt)
     gene            complement(629..710)
                     /locus_tag="VBE22_002052"
     tRNA            complement(629..710)
                     /locus_tag="VBE22_002052"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(674..676),aa:Leu,seq:tag)
     gene            complement(720..792)
                     /locus_tag="VBE22_002053"
     tRNA            complement(720..792)
                     /locus_tag="VBE22_002053"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(757..759),aa:Lys,seq:ttt)
     gene            complement(795..865)
                     /locus_tag="VBE22_002054"
     tRNA            complement(795..865)
                     /locus_tag="VBE22_002054"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Asp,seq:gtc)
BASE COUNT          236 a          251 c          196 g          182 t
ORIGIN      
        1 ggaccttgta ggactcgaac ctacgaccgc tcggttatga gccgagtgct ctaaccagtt
       61 gagctaaagg tccaaagtct caataaaata aatagcggcg gaggggatcg aacccccgac
      121 ctcccgggta tgaaccggac gctctagcca gctgagctac accgccataa aaatatatcc
      181 atcgggaaga caggattcga acctgcgaca ccttggtccc aaaccaagta ctctaccaag
      241 ctgagctact tcccgaaaaa tatgcaccct agaggagtcg aacctctaac cgcctgattc
      301 gtagtcaggt actctatcca gttgagctaa gggtgctaaa tattatatgc cgaggaccgg
      361 aatcgaaccg gtacgatgtt taccatcgca ggattttaag tcctgtgcgt ctgccagttc
      421 cgccaccccg gcctctaaca agcgaacgac ggggttcgaa cccgcgaccc tcaccttggc
      481 aaggtgatgt tctaccactg aactacgttc gcactaaaga cactatttat cctataaaat
      541 tgtaatgccg gctacatgac ttgaacacgc gaccctctga ttacaaatca gatgctctac
      601 caactgagct aagccggcaa tctactaatg cgggtgaagg gacttgaacc cccacgccgt
      661 taagcgccag atcctaaatc tggtgcgtct gccaattccg ccacacccgc atttctaaat
      721 gacccgtact gggctcgaac cagtgaccca ttgattaaaa gtcaattgct ctaccaactg
      781 agctaacgag tctacggtcc cgacgggaat cgaacccgcg atcttcgccg tgacagggcg
      841 acgtgataac cgctacacta cggga
//
LOCUS       DATRTN010000188          819 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.195, whole genome shotgun sequence.
ACCESSION   DATRTN010000188 DATRTN010000000
VERSION     DATRTN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.195"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(133..>819)
                     /locus_tag="VBE22_002055"
     CDS             complement(133..>819)
                     /locus_tag="VBE22_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEO7203446.1"
                     /translation="VIFGGKAAPAYTIAQDIIHLILCLSELINNDPEVNKYLNVHLVE
                     NYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAELAG
                     KENIYTFGKDSDTIINLYETSGYRSKDYYDKDKVIREAVDFIISDDIVSLGNAERLKR
                     LHDELVGKDWFMTLIDLKEYIAVKEQVLADYEDYESWNKKVIHNIAKAGFFSSDRTIE
                     QYNQDIWHSN"
BASE COUNT          272 a          146 c          114 g          287 t
ORIGIN      
        1 tacacatgca attaaatatt tagaataagt tctgctcagt tctattttta aagtctacgg
       61 acaaaaataa atacttatag ttttattaga caagaaaaaa gtaaggttga aaaccttact
      121 tttagagact ttttaatttg aatgccaaat atcttgatta tattgttcga ttgtacgatc
      181 tgatgagaag aaccctgctt tagcaatatt gtgtataact ttcttattcc atgattcata
      241 atcttcataa tcagcaagca cttgttcttt aacagcaatg tattctttca aatcaatcaa
      301 tgtcatgaac caatctttac ctactaactc atcatgcaat ctctttagtc gttctgcatt
      361 accgagtgac actatatcat cactaatgat aaaatcaaca gcttcacgaa ttactttgtc
      421 tttatcatag taatcttttg aacgataacc agatgtttca taaagattaa tgatagtatc
      481 agagtcttta ccaaatgtgt agatgttctc tttgccagct aattcagcta tttcaacatt
      541 tgccccatcc atagtaccaa gagtaagagc accattcaac ataaatttca tgttacctgt
      601 accagatgct tctttggaag ccaatgaaat ttgttccgaa atatctgttg cagggattaa
      661 cttttccgct actgtcacat tgtaattttc tactaaatgg acattaaggt atttattaac
      721 ttctggatca ttattaatta attctgataa gcagagaatt aagtgaatga tatcctgtgc
      781 aatagtatag gctggagcag ctttaccacc aaaaataac
//
LOCUS       DATRTN010000189          815 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.196, whole genome shotgun sequence.
ACCESSION   DATRTN010000189 DATRTN010000000
VERSION     DATRTN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.196"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..87
                     /locus_tag="VBE22_002056"
     CDS             <1..87
                     /locus_tag="VBE22_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEO7203447.1"
                     /translation="ELKQRIINALMRKGYQYEDIKSALREYL"
     gene            171..704
                     /locus_tag="VBE22_002057"
     CDS             171..704
                     /locus_tag="VBE22_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEO7203448.1"
                     /translation="MRLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRETGVSYYCNLASPYILDPEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEQHKAQMNYPTDIDYILKENVKILVEWINENKGPFS
                     SSYINIWYKRYLELKKR"
BASE COUNT          314 a          120 c          139 g          242 t
ORIGIN      
        1 gaattaaagc aacgtataat caatgcctta atgcgtaaag gataccagta cgaagatatc
       61 aaaagtgctt taagagaata tttataagat aataactctc agacgatgta ttttacagaa
      121 aattatgata aactataaca gacgtataaa ttgtagaaag ttggtaggct atgagattac
      181 ctaaagaagg cgactttatt acaattcaaa gttacaaaca tgatggtagt ttacaccgga
      241 cttggcgtga caccatggta ttaaaaacaa ccgaaaatgc cctcattggt gttaatgatc
      301 atactttagt aacagaaaat gatggtcgac gctgggtgac acgagagcct gcaatagtat
      361 actttcataa aaaatactgg tttaacatta tcgctatgat acgtgaaact ggtgtctcct
      421 actattgtaa tctagcaagt ccgtatatct tagaccctga agcactcaag tatattgact
      481 atgaccttga tgtcaaagta tttgctgatg gtgaaaaaag actactagat gtggacgaat
      541 atgaacagca taaagctcag atgaactatc ctaccgatat tgattatata ttaaaggaaa
      601 atgtaaaaat attggtagaa tggataaatg agaataaagg ccccttttca tcatcatata
      661 tcaatatctg gtataaacgg taccttgaat tgaaaaagcg ctaactaaca cactaaatag
      721 tgtgttttta ttaatatcaa atttaattac aatactattg caaaaatata tacttaaaat
      781 aaaaaaagta gaaagtgatc tttctacttt tttaa
//
LOCUS       DATRTN010000190          806 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.197, whole genome shotgun sequence.
ACCESSION   DATRTN010000190 DATRTN010000000
VERSION     DATRTN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.197"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            21..>806
                     /locus_tag="VBE22_002058"
     CDS             21..>806
                     /locus_tag="VBE22_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEO7203449.1"
                     /translation="MATYRQRGKKKLWDYRIFNEKSELVASGSGFKTKREAMNEAMRI
                     EQQKLLVNSISSDITLYDLWFEWYSLIIKPSNLAETTKNKYFTRGSVIRKLFGNQKVN
                     KIKHSAYQRKLNMYAEKYTKNHVRRLNSDIKKAIQFAKRDGVLLSDFTDGVVIAGRKF
                     VKDADDKYLHSISDYKKVISYLENNLDYSNSIVYYLLLVLFKTGLRVGEALALTWDDV
                     NFEDLEIKTYRRFSGDKGTFSPPKTKTSIRTIPISQSLALTLRR"
BASE COUNT          305 a           88 c          156 g          257 t
ORIGIN      
        1 atgtctaaga aaggagtaaa atggcaacat atagacaaag aggtaaaaag aaattatggg
       61 attatagaat attcaatgaa aaaagtgagt tagttgcttc tggttcaggt tttaagacaa
      121 agcgtgaagc tatgaatgaa gctatgagga tagagcaaca aaagttatta gtaaattcta
      181 tatcttctga tataacattg tatgatttgt ggtttgaatg gtacagttta attataaagc
      241 ctagcaatct agcagagact acaaagaaca aatattttac aagaggttct gttattcgta
      301 aactttttgg aaatcagaaa gtaaataaaa taaagcatag tgcgtatcag aggaaattaa
      361 atatgtacgc tgaaaaatac actaaaaacc atgttagaag gttaaattca gatataaaaa
      421 aagctatcca atttgctaaa agagatggtg tcttattatc tgattttact gatggagttg
      481 tcatagctgg taggaaattt gttaaggatg cagatgataa atatctacat agtatttctg
      541 attacaagaa agtgatatct tatttagaaa acaatttgga ttattcaaat agtattgttt
      601 attatttatt actggtgcta tttaaaacag gtttacgagt gggggaagct ttagctttaa
      661 cttgggatga tgttaatttt gaagatctag aaatcaagac gtatagaaga tttagtgggg
      721 ataagggaac ttttagtcca cctaaaacaa aaacctcaat tcgtactatt cctattagtc
      781 aatctttggc attaacctta agacgt
//
LOCUS       DATRTN010000191          799 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.198, whole genome shotgun sequence.
ACCESSION   DATRTN010000191 DATRTN010000000
VERSION     DATRTN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.198"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..274
                     /locus_tag="VBE22_002059"
     CDS             <1..274
                     /locus_tag="VBE22_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEO7203450.1"
                     /translation="KLVQQYYSYSISTLVFYTQLESGNYEGPSKVLVASRELMNGNKL
                     RLFLLDLSFIGWHFLTIFSFGLVYIYLLPYQTTARLIFYRNITKNS"
     gene            complement(288..>799)
                     /locus_tag="VBE22_002060"
     CDS             complement(288..>799)
                     /locus_tag="VBE22_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO7203451.1"
                     /translation="SFITLETMSDTAEPHKEETIYLDKLLIDILSEFQLVFEKENRQV
                     MIDVAPDVSKLSSQYDKLSRILLNLISNAVKYSDPGSPLTIKAYSNRQDIVIDIIDQG
                     YGIKDEDLASIFNRFYRVESSRNMKTGGHGLGLYIARQLAHQLNGDILVESQYQKGSK
                     FSLVLKLQK"
BASE COUNT          263 a          148 c          114 g          274 t
ORIGIN      
        1 aaaactcgtt caacaatatt actcctatag tatctcaact ttagtatttt acactcaatt
       61 agaaagtgga aactatgagg gaccatctaa agtactagtt gctagtcgag aactaatgaa
      121 tggtaataaa cttagactct tccttttaga cttaagtttt attggttggc atttccttac
      181 aatcttcagt ttcggattag tctatatcta tctccttcct taccaaacta ctgcgcgctt
      241 aatcttttat agaaacatta caaaaaatag ctaggaataa ttcctagcta tttttgtaat
      301 ttaagtacta aagagaactt actgcctttt tgatactgac tttcgaccaa aatatcacca
      361 ttcagttgat gtgcaagttg tcgcgcaata taaagaccta ggccatgtcc tcctgtcttc
      421 atatttcgtg aagattctac acgataaaaa cgattaaaaa tagaagcaag atcttcatct
      481 tttatcccat agccttggtc aataatatcg ataacgatat cttgacgatt tgaataggcc
      541 ttgatagtca agggacttcc aggatcagaa tatttgactg cattgctaat caaattcaat
      601 aaaattcgtg ataatttatc atattgacta gataatttcg aaacatctgg cgcaacatca
      661 atcattactt gccgattttc tttttcaaac accaattgaa attcagataa aatatcaatc
      721 agtagtttat ccagataaat tgtttcttct ttatgaggtt cagccgtgtc tgacatagtc
      781 tctaatgtaa taaaactta
//
LOCUS       DATRTN010000192          730 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.201, whole genome shotgun sequence.
ACCESSION   DATRTN010000192 DATRTN010000000
VERSION     DATRTN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.201"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(181..>730)
                     /locus_tag="VBE22_002061"
     CDS             complement(181..>730)
                     /locus_tag="VBE22_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203452.1"
                     /translation="KNMNQLGLVILVFLFSSTLTQEFSKGTLINLVTKGLAKKVIILA
                     KFIVITLLWTVSYLLSVVIHFSYTLYYFSNEGSHKLMVYGATWFIGILFISLILFFSV
                     LFRKTLGGLLGCLFMVVLCVVSGFFKISKSYNPMLLIQKQGEILSGKFKVQDLMEPSI
                     LVFLLIIMTLLLSMYIFEKSEV"
BASE COUNT          278 a          117 c           87 g          248 t
ORIGIN      
        1 tttaatttcc ataatatatt tccttatttt gactttaaag tatatttctg caatgaggta
       61 tgcataggat atcatattag ttggaaatat tgatctagat aagtcaaata gcagcaaagc
      121 tcttaattcg gttttatttt ttcaaaaagt agctatcgcc ttattgatat cttatcaaaa
      181 ttaaacttca cttttttcaa aaatgtacat gctaagtaat aacgtcatta taataagcaa
      241 aaacactaat atgctcggtt ccattaagtc ttgaacttta aactttccac ttaatatttc
      301 tccttgtttt tggataagaa gcataggatt atagctttta ctgattttaa aaaatccaga
      361 tactacacat aacaccacca taaaaagaca acctaacaaa ccccctaaag ttttacgaaa
      421 aagaactgaa aaaaagagta ttaagcttat gaaaagaata ccgataaacc atgttgcacc
      481 gtaaaccatt aatttatgac ttccttcgtt actaaagtaa tataacgtat aactaaaatg
      541 gatgacaaca cttaataagt atgacactgt ccatagaagg gtaattacta taaatttagc
      601 aaggattata acttttttgg ctaatccctt cgtaactaga ttaattaagg ttccttttga
      661 aaattcttgc gtcaatgtac tactaaataa aaatactaaa ataactaaac caagctgatt
      721 catattttta
//
LOCUS       DATRTN010000193          681 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.202, whole genome shotgun sequence.
ACCESSION   DATRTN010000193 DATRTN010000000
VERSION     DATRTN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.202"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..305
                     /locus_tag="VBE22_002062"
     CDS             <1..305
                     /locus_tag="VBE22_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203453.1"
                     /translation="IMLIIFIVFSGIYYFYYLPKNQGKKWKFNQWKLQKGKKEQLKIN
                     YGIIFGLVMLSPVFLVGSIENIFGLIGGVLFTATFPALIVDSLYATYYIHKHPEYE"
     gene            545..681
                     /locus_tag="VBE22_002063"
                     /pseudo
     CDS             545..681
                     /locus_tag="VBE22_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926392.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
BASE COUNT          234 a           73 c          133 g          241 t
ORIGIN      
        1 tgataatgtt aattattttt attgtatttt ctggaatata ttatttctat tatttaccaa
       61 aaaatcaggg gaaaaaatgg aaatttaatc agtggaaatt acaaaaagga aaaaaggaac
      121 aattaaaaat taattatgga attatttttg gtttagtgat gctttctcca gtctttcttg
      181 taggtagtat tgaaaatatt tttggtttga ttggaggtgt attatttacg gcaacttttc
      241 cagctctgat tgtcgattcc ctttatgcaa catattatat tcataagcat cccgaatatg
      301 agtgataaat taattaattt aaggtaggag caataaatat ctagaaaaaa tgaagtaggt
      361 agaatagtct ctacgcttgc tgagagtgtt tttgtagtaa tcataacagc tccaatttgt
      421 accagtagta gcagttgtta taggtacttt ggccattgtg gagtaaaagc actctatgaa
      481 aatgttaaag atgtaagaaa ttttgtggag ccatttcatg agcaaatatt attgatcaag
      541 atgatatttt gattatctgg caggattttt ccagagagct caatttagaa caagtatatt
      601 atttcaatga ggaagatatt gatgaagtaa tttttggagg taccatgacg aagagcaaga
      661 aagagtagct cgtgtcattg a
//
LOCUS       DATRTN010000194          678 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.203, whole genome shotgun sequence.
ACCESSION   DATRTN010000194 DATRTN010000000
VERSION     DATRTN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.203"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..675)
                     /locus_tag="VBE22_002064"
     CDS             complement(<1..675)
                     /locus_tag="VBE22_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-like surface protein"
                     /protein_id="HEO7203454.1"
                     /translation="MFRRSKNNSYDTSQTKQRFSIKKFKVGAASVLIGLSFLGGVTQG
                     NLNIFEESIVAASTIPGSAATLNTSITKNIQNGNAYIDLYDVKLGKIDPLQLIVLEQG
                     FTAKYVFRQGTKYYGDVSQLQSTGRASLTYNIFGEDGLPHVKTDGQIDIVSVALTIYD
                     STTLRDKIEEVRTNANDPKWTEESRTEVLTGLDTIKTDIDNNPKTQTDIDSKIVEVNE
                     LEKLLVL"
BASE COUNT          210 a          140 c           82 g          246 t
ORIGIN      
        1 caatactaac aatttctcta attcattaac ctcaacaatt ttactatcaa tatctgtttg
       61 cgtcttagga ttattatcaa tatctgtctt aattgtatct aatcctgtta aaacctcagt
      121 acgactttct tccgtccact taggatcgtt tgcattcgtt ctaacttctt caatcttatc
      181 cctcaaggtt gttgaatcat aaatagttaa agcaacacta actatatcaa tttgtccatc
      241 agtctttaca tgtggtagtc catcttcacc aaatatatta taggtaagac tagcccttcc
      301 tgtactctgc aactgagaaa catccccata gtatttagta ccttgtctaa aaacatactt
      361 tgctgtaaaa ccttgttcta aaacaattaa ttgtaatgga tctattttac ctaattttac
      421 atcatataaa tctatgtaag catttccgtt ttgtatattt ttagtgatgc ttgtatttaa
      481 ggtcgctgca ctccctggaa ttgtagatgc agcaactatt gactcttcaa aaatattaag
      541 attaccttgt gtaaccccac ccaaaaaact aagaccaatt agtacagaag ctgcaccaac
      601 cttgaacttc ttaattgaaa accgttgttt cgtctgtgaa gtatcataac tgttattttt
      661 agaccttcta aacataat
//
LOCUS       DATRTN010000195          673 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.204, whole genome shotgun sequence.
ACCESSION   DATRTN010000195 DATRTN010000000
VERSION     DATRTN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.204"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..>673
                     /locus_tag="VBE22_002065"
     CDS             <1..>673
                     /locus_tag="VBE22_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HEO7203455.1"
                     /translation="FSQYLKDHSPTISFIQKDGVRVMLEGNLQIVDKPRSKASLSNEM
                     LVPIEWKKYGTNIRKESQGVAKDRASIQYVTINKLINTDELIVFDDDGSGEIADIVTI
                     SANEAERKILINLYHCKYSHGDEPGARVSDLYEVCGQAEKSIIWKDNLLDFLDRMIKR
                     ESIRISKRQSSRFEKGNINDCRTIKSMIKDGFSTDMRITIVQPGVSISKLSTEMKQLL
                     LSTEYY"
BASE COUNT          249 a           81 c          141 g          202 t
ORIGIN      
        1 ttttcacaat atttaaaaga tcatagtcca actatttctt tcatacagaa ggatggggtt
       61 cgggtaatgt tagagggtaa tctacaaatt gtagataaac cgaggagtaa ggcatcgttg
      121 tcaaatgaga tgcttgttcc gatagaatgg aaaaaatatg gaacaaatat acgaaaagag
      181 tctcaaggtg ttgctaagga tagagcatcc attcaatatg taactataaa taaattgata
      241 aatactgatg aattgatagt ttttgacgat gatggttcag gagaaattgc agacattgtt
      301 acaataagtg ctaatgaagc agagcgcaaa atactcataa atctgtatca ttgtaaatat
      361 tcacatggtg atgaaccagg tgctcgtgta tcagatttat acgaagtgtg tggtcaagca
      421 gagaaatcaa ttatttggaa agataattta ttagatttcc ttgatagaat gataaaaaga
      481 gagtcaatac ggatttctaa aagacagagt tctcggtttg aaaaaggaaa tattaatgat
      541 tgtcggacaa tcaaatctat gataaaagat ggctttagta ctgatatgag aataaccatt
      601 gtacagccag gtgtgtcgat ttcgaaacta agtactgaga tgaagcaatt gttattatca
      661 actgagtatt att
//
LOCUS       DATRTN010000196          629 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.210, whole genome shotgun sequence.
ACCESSION   DATRTN010000196 DATRTN010000000
VERSION     DATRTN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.210"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(406..>629)
                     /locus_tag="VBE22_002066"
     CDS             complement(406..>629)
                     /locus_tag="VBE22_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEO7203456.1"
                     /translation="KAVVGTINLDYRSLYHHFECAVYLYKVDAIQDIYRDYMDTLNKS
                     RLVSLKDINNIPKFQKVIGIVTKTIAPLL"
BASE COUNT          220 a           98 c           91 g          220 t
ORIGIN      
        1 ctagattatt taaaaatttt acaaagaatg tgatggtagc atattcaatt aaagttaagt
       61 aaagacataa tttttagaag gaaattgtga cttatttatg caaaaaaaag aaagaaccag
      121 tttggttctt tcttttttgc ttttcagcta cttaccacag tttgtaagaa attacaagta
      181 gtagtaagct aagggcacct tttcaagtgt gataagaagg ttcacacaac ttatcaagat
      241 ccgctcctgc gtttataatc tccctgcgca atagtggtaa aaccacatat cgactcatcg
      301 cataacagta gtttaacata atttagttaa aatgcaacct ttacttttta aatataacta
      361 gattcttcag tttactttta atattaaatt aaaaataaat tatttctaaa gcaggggagc
      421 gatagttttt gtcactatgc caattacttt ttggaatttt ggaatattat ttatatcttt
      481 caagctaact aaccttgatt tatttaacgt atccatataa tctcgataaa tgtcttggat
      541 tgcatctacc ttataaagat aaacagcaca ttcaaaatgg tgatacaggc tacggtaatc
      601 aagatttatc gtacctacaa cagcctttg
//
LOCUS       DATRTN010000197          589 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.212, whole genome shotgun sequence.
ACCESSION   DATRTN010000197 DATRTN010000000
VERSION     DATRTN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.212"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..548)
                     /locus_tag="VBE22_002067"
     CDS             complement(<1..548)
                     /locus_tag="VBE22_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HEO7203457.1"
                     /translation="MPLLIHSLILSVLPFLMWLTLVNRDKPLYKTIWSILLGLQLITI
                     YTWFFWAKLPLSESLPLYHCRIGMFVVLLARPGILKDYFALLGVVGGVLAMIHPDFYP
                     YQFLHVTNIFFFIGHFALFVLSLLHLMTQSNLDKLNPKLIIQLTLLINMSLIFINLLT
                     GGNYGFMMKTPILGITNPFLNL"
BASE COUNT          231 a           97 c          102 g          159 t
ORIGIN      
        1 aaaaggttta aaaagggatt tgttattcct agtattggtg tcttcatcat aaagccataa
       61 tttcccccag tcaagaggtt aataaaaatc aaagacatat taatgagaag cgttagttgt
      121 ataatcaatt ttggatttaa tttgtccaaa ttggactgtg tcatcagatg tagtaaacta
      181 aggacaaata aagcaaaatg tccaataaag aagaaaatat tagtcacatg tagaaactgg
      241 taagggtaaa agtctgggtg aatcattgcc aatactccac ctactactcc taacaatgcg
      301 aaataatcct ttaatattcc aggcctcgct aagagaacga caaacatgcc tattcgacaa
      361 tggtaaaggg gaagactttc agataaaggc aattttgccc agaaaaacca agtataaatc
      421 gtaattaact gaagtcctaa aaggatactc caaatagttt tatacaaagg cttatctcta
      481 ttaaccaaag tcagccacat cagaaaaggt aagacagata aaatcaaact atggataagt
      541 aaaggcatct gcggaatctc tgttctgata ttagaaaaga attctatca
//
LOCUS       DATRTN010000198          557 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.213, whole genome shotgun sequence.
ACCESSION   DATRTN010000198 DATRTN010000000
VERSION     DATRTN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.213"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            1..557
                     /locus_tag="VBE22_002068"
                     /pseudo
     CDS             1..557
                     /locus_tag="VBE22_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          194 a          104 c          113 g          146 t
ORIGIN      
        1 acccctaagt taagcttaga agatctcctc atggctactc ttcaatacat gcgagaatac
       61 cgcacttatg aacaaattgc ggctgatttt ggcattcacg aaagcaactt aatccgtcga
      121 agtcaatggg ttgaatcaac tcttattcaa agtggtttta cgatttcaaa aactcatctt
      181 agtgctgagg atacggtgat tgtggatgca acagaggtaa aaatcaatcg tcctaaaaaa
      241 atcaactagc gaattattct ggtaaaaaga aatgtcatgc tatgaaggct caggcgattg
      301 tcacaagcca agggagaatt gtttctttgg atattgcagt gaactattgc cacgatatga
      361 agttgttcaa aatgagtcgc agaaacatcg gacaagctgc taaaatcttg gcagacagtg
      421 gttatcaagg gatcatgaag atgtattcac aagcgcaaac tccgaggaaa tcaagcaaac
      481 ttaagccact aactcttgaa gataaaacct ataaccatac gctatccaaa gagagaatca
      541 aggttgagaa tattttt
//
LOCUS       DATRTN010000199          541 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.216, whole genome shotgun sequence.
ACCESSION   DATRTN010000199 DATRTN010000000
VERSION     DATRTN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.216"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            76..198
                     /locus_tag="VBE22_002069"
     CDS             76..198
                     /locus_tag="VBE22_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEO7203458.1"
                     /translation="MFKGFKKLIIIMGGISLMMILTACDLVKKITNGFKNIENT"
BASE COUNT          210 a           76 c           92 g          163 t
ORIGIN      
        1 tgagttggtt gtttgcgata gcaacgtcaa gacagcaata aaattgaaaa aatcaataag
       61 ataagaggaa aagtgatgtt taaaggattt aaaaaattga ttattattat gggagggata
      121 agcttaatga tgattttaac agcatgtgac ttagtaaaaa aaatcaccaa tgggttcaag
      181 aacattgaaa atacttagaa gctggatcaa aagttatcaa agaagtcagc caaaacattc
      241 aaccagttat ttctgatctg aatagctatc tcaatggagc ggcaagtgct tttgagcaat
      301 cggattcgag tgcagctact catattaaaa ataacaccat cgcccaattt taaaaagcag
      361 taccaaatcg tgtgagaaat taccaggcta attgtgagtg agaatctatt gttctcactt
      421 ttttattatc aaaacgctta aattctttac ttagacctca cctacacact tcagataatg
      481 ggtgttacat attaattaat aaatatgtaa aaaataatat ttgatatgct ataataataa
      541 t
//
LOCUS       DATRTN010000200          525 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.219, whole genome shotgun sequence.
ACCESSION   DATRTN010000200 DATRTN010000000
VERSION     DATRTN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.219"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..370
                     /locus_tag="VBE22_002070"
     CDS             <1..370
                     /locus_tag="VBE22_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO7203459.1"
                     /translation="SNYYYLLTIISNIVLNAVEAIDKQKKGTISVHTEELEDFIKIEI
                     SDNGPGIPDKMKHMIFKPGFSTKFDANGDIYRGIGLSHVRILMEEQYQGTITVCPNQP
                     NGTTFTLLFRKNRFKLGDNT"
     gene            367..>525
                     /locus_tag="VBE22_002071"
     CDS             367..>525
                     /locus_tag="VBE22_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203460.1"
                     /translation="MKFYIIDDDPTITMILQDIIEEDFNNTVVRVNNVSSKAYNELLI
                     ADVDIVLID"
BASE COUNT          195 a           77 c           90 g          163 t
ORIGIN      
        1 atcaaactat tactatttac taaccattat ttccaatatc gttttaaatg cagtagaagc
       61 tattgataaa caaaaaaagg gtacaatcag tgttcacaca gaagaattag aagattttat
      121 taaaattgag attagtgata acggaccagg aatacctgat aaaatgaaac acatgatttt
      181 caaacctgga ttttcgacta aatttgatgc taatggtgat atctatcgag gaatcgggct
      241 atcccacgtg cgtattttaa tggaagagca atatcaagga acgataactg tttgtcctaa
      301 tcagccaaat ggaacaacat ttacactgtt attccgtaaa aatcgattta agttaggaga
      361 taatacgtga aattctatat tattgatgat gaccccacta tcactatgat tttacaagat
      421 attattgaag aagatttcaa taatacagta gtcagagtga ataatgtctc cagtaaagca
      481 tacaacgaat tgctgattgc agatgttgat atcgtattga ttgat
//
LOCUS       DATRTN010000201          522 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.220, whole genome shotgun sequence.
ACCESSION   DATRTN010000201 DATRTN010000000
VERSION     DATRTN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.220"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..>522
                     /locus_tag="VBE22_002072"
     CDS             <1..>522
                     /locus_tag="VBE22_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEO7203461.1"
                     /translation="KKFLITFVAIAFATAGLFYSLFIVTPQYTSSTRIYVINPNTPNN
                     SITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKLNYPSSQLLQKITVSILKDTRVI
                     SISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDITTLEKGNLPKAPSSPNIKKNVL
                     IGFIVGAGLSTIV"
BASE COUNT          178 a           88 c           81 g          175 t
ORIGIN      
        1 aaagaaattt ttaatcacat ttgttgccat tgcatttgca acagctggac ttttctatag
       61 tctttttatt gttacgccac aatatacttc ttcaacaagg atatatgtca ttaaccctaa
      121 tacccctaat aatagtatta ctgcacaaga tctacaagcg gggagttttc ttgcaaatga
      181 ctataaggag attattacgt ctactgacgt tctagaaaaa gttatttctt ctgaaaaatt
      241 gaattatcct tcgtctcagt tgttgcaaaa aataacagtt tctattttaa aagatacacg
      301 tgttatttca atatcggtcg aagatgctaa tccaaaaatg tctcaaaaat tagcaaattc
      361 agttagagaa gcagcagttt caaaaatcaa ggcagttact caagtagagg atatcactac
      421 tcttgagaag ggaaatttac ctaaagcacc atcttctcct aatattaaaa agaatgtact
      481 aatcgggttt attgttggtg caggattatc aactattgtt tt
//
LOCUS       DATRTN010000202          521 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.222, whole genome shotgun sequence.
ACCESSION   DATRTN010000202 DATRTN010000000
VERSION     DATRTN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.222"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            <1..>521
                     /locus_tag="VBE22_002073"
     CDS             <1..>521
                     /locus_tag="VBE22_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISSag2 family transposase"
                     /protein_id="HEO7203462.1"
                     /translation="VESVSETEFEETIKRIFLDSESRYGSRKIKICLNNEGITLSRRR
                     IRRIMKRLNLVSVYQKATFKPHSRGKNEAPIPNHLDRQFKQERPLQALVTDLTYVRVG
                     NRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFHSDRGKEFDN
                     QLIDEILEAFGIT"
BASE COUNT          165 a          116 c          102 g          138 t
ORIGIN      
        1 cgtggagtca gtatctgaaa cggagtttga agaaactatt aaaagaattt ttctcgatag
       61 cgagtctaga tacggatcca gaaaaatcaa aatatgcttg aataacgaag gtatcacact
      121 ttcacgtcgt cggattcgac gcattatgaa gcgactcaat ttggtttctg tttatcagaa
      181 agccaccttc aaaccacatt ctagaggcaa gaatgaagcc cctattccca accacttaga
      241 caggcaattt aagcaagaaa gaccactaca agccttagtc actgacttaa cctatgttcg
      301 tgtaggcaat cgttgggctt atgtttgcct catcattgac ctatacaacc gtgaaatcat
      361 cggcctgtct cttggttggc acaagaccgc tgaactcgtt aagcaagcca tacaaagcat
      421 cccttacgcc ctgaccaaag tcaagatgtt ccattcagat cgtggcaaag agtttgataa
      481 tcagttaatt gatgaaatat tggaagcctt tggaatcaca c
//
LOCUS       DATRTN010000203          515 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.224, whole genome shotgun sequence.
ACCESSION   DATRTN010000203 DATRTN010000000
VERSION     DATRTN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.224"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..509)
                     /locus_tag="VBE22_002074"
     CDS             complement(<1..509)
                     /locus_tag="VBE22_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO7203463.1"
                     /translation="MNKFFLESERLIIRPLEESDYDSWYTSFMNRGPSLTPFDDGILD
                     MSMCDINWFKNLVNLQRKEWESDIVYIFGVFLKSGEHVGMLNIVTLARADMQWGELGY
                     VFHNQFWSNGYAFESILALLNSTYEKLGFHHIEAQITPGNERSENLVRRLGLTYETTR
                     KDFSFENGK"
BASE COUNT          175 a          109 c           52 g          179 t
ORIGIN      
        1 cattttccat tctcaaatga aaaatctttt cgagttgtct cataggttaa tcccaatctt
       61 ctaaccaaat tttccgatcg ttcattccca ggagtaatct gtgcttcaat atgatgaaaa
      121 cctaattttt cataagtact attaagtaat gccaagattg actcaaatgc atagccatta
      181 ctccaaaatt gattatgaaa aacatatccc aattctcccc attgcatatc tgctcttgca
      241 agggtaacaa tatttaacat tcctacgtgc tcaccgcttt ttagaaaaac accaaatata
      301 tatactatgt cactttccca ttctttcctt tgtaaattta ctaaattttt aaaccaatta
      361 atgtcacaca tagacatatc aagtatccca tcatcaaatg gagttaaaga tggtccacga
      421 ttcataaaac tagtatacca actatcataa tcactttctt ctagtggcct tataataagt
      481 ctctctgatt ctaaaaaaaa tttattcaat tttta
//
LOCUS       DATRTN010000204          472 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.denovo.231, whole genome shotgun sequence.
ACCESSION   DATRTN010000204 DATRTN010000000
VERSION     DATRTN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.denovo.231"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            complement(<1..469)
                     /locus_tag="VBE22_002075"
     CDS             complement(<1..469)
                     /locus_tag="VBE22_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO7203464.1"
                     /translation="MSIIIGLMAAMFIIRLAYLKLSIANEKALRKNGAKEYGVGVSKA
                     ITVLHIIIYFSSVTEAILTKASFNFVSVIGLSLMIFSVFMLHTVTRLLGRIWTVKLMV
                     DKNHQFVDHWLFRVVKHPNYFLNIAPELLGVTLLCHAKYTALFVLPIYAFVIYL"
BASE COUNT          189 a           80 c           64 g          139 t
ORIGIN      
        1 ttaaataaat aacaaaagca taaattggca agacaaataa tgctgtgtat ttagcatgac
       61 acaataaagt tacacctaaa agttcaggag caatatttaa gaaatagtta ggatgcttaa
      121 caaccctaaa tagccagtga tctacaaatt gatgattttt atctaccatt aatttcactg
      181 tccaaatacg accaagtaaa cgtgttactg tatgtaacat aaacacagaa aaaatcatca
      241 agctaagccc gattacgcta acaaaattaa aacttgcttt tgttagaata gcctcagtta
      301 cagatgaaaa ataaatgata atatgaagaa ctgtgattgc ttttgaaaca cctacaccat
      361 attctttagc accatttttt cttaaggcct tttcattagc tatagatagc tttaaataag
      421 ctaaacgaat gataaacata gcggccatta acccaataat aattgacatt gt
//
LOCUS       DATRTN010000205         3894 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699452-rid18550053.guided.124, whole genome shotgun sequence.
ACCESSION   DATRTN010000205 DATRTN010000000
VERSION     DATRTN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699452
            Sequence Read Archive: SRR7788323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001789836.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001789836.1
            Genome Coverage       :: 118x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:24:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,080
            CDSs (total)                      :: 2,035
            Genes (coding)                    :: 1,987
            CDSs (with protein)               :: 1,987
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 48
            CDSs (without protein)            :: 48
            Pseudo Genes (ambiguous residues) :: 0 of 48
            Pseudo Genes (frameshifted)       :: 31 of 48
            Pseudo Genes (incomplete)         :: 11 of 48
            Pseudo Genes (internal stop)      :: 10 of 48
            Pseudo Genes (multiple problems)  :: 4 of 48
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699452-rid18550053.guided.124"
                     /serotype="Ia"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2008-06-09"
                     /collected_by="RMPRU"
     gene            323..439
                     /locus_tag="VBE22_002076"
     CDS             323..439
                     /locus_tag="VBE22_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO7203465.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            455..2374
                     /gene="tet(M)"
                     /locus_tag="VBE22_002077"
     CDS             455..2374
                     /gene="tet(M)"
                     /locus_tag="VBE22_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO7203466.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2472..2660
                     /locus_tag="VBE22_002078"
     CDS             2472..2660
                     /locus_tag="VBE22_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO7203467.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2718..3071)
                     /locus_tag="VBE22_002079"
     CDS             complement(2718..3071)
                     /locus_tag="VBE22_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO7203468.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARIKRGLTREQVGTIIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQIEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3603..>3894
                     /locus_tag="VBE22_002080"
     CDS             3603..>3894
                     /locus_tag="VBE22_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO7203469.1"
                     /translation="MKPSSFQETIENQFDYICKQAIEDERKDYFKHLSRLSKKEVSFS
                     EIDNYLVTSFSTVDTYVTDFQLFTLYGLRINVESDLLSEALRHLPENKRNIIL"
BASE COUNT         1329 a          616 c          714 g         1235 t
ORIGIN      
        1 caagaaacaa aggcaaccca aatctcgcaa tttgagttga tattagaaaa acaagataat
       61 tggaagattg taaaataaca aatattggta catgattaca gatactttgt aatcatgtac
      121 tctttttgat aaaaaattgg agattccttt acaaatatgc tcttacgtgc tattatttaa
      181 gtgactattt aaaaggagtt aataaatatg cggcaaggta ttcttaaata aactgtcaat
      241 ttgatagcgg gaacaaataa ttagatgtcc ttttttagga gggcttagtt ttttgtaccc
      301 agtttaagaa tacctttatc atgtgattct aaagtatcca gagaatatct gtatgctttg
      361 tatacctatg gttatgcata aaaatcccag tgataaaagt atttatcact gggattttta
      421 tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa attattaata ttggagtttt
      481 agctcatgtt gatgcaggaa aaactacctt aacagaaagc ttattatata acagtggagc
      541 gattacagaa ttaggaagcg tggacaaagg tacaacgagg acggataata cgcttttaga
      601 acgtcagaga ggaattacaa ttcagacagg aataacctct tttcagtggg aaaatacgaa
      661 ggtgaacatc atagacacgc caggacatat ggatttctta gcagaagtat atcgttcatt
      721 atcagtttta gatggggcaa ttctactgat ttctgcaaaa gatggcgtac aagcacaaac
      781 tcgtatatta tttcatgcac ttaggaaaat ggggattccc acaatctttt ttatcaataa
      841 gattgaccaa aatggaattg atttatcaac ggtttatcag gatattaaag agaaactttc
      901 tgccgaaatt gtaatcaaac agaaggtaga actgtatcct aatatgtgtg tgacgaactt
      961 taccgaatct gaacaatggg atacggtaat agagggaaac gatgaccttt tagagaaata
     1021 tatgtccggt aaatcattag aagcattgga actcgaacaa gaggaaagca taagatttca
     1081 gaattgttct ctgttccctc tttatcatgg aagtgcaaaa agtaatatag ggattgataa
     1141 ccttatagaa gttattacta ataaatttta ttcatcaaca catcgaggtc cgtctgaact
     1201 ttgcggaaat gttttcaaaa ttgaatatac aaaaaaaaga caacgtcttg catatatacg
     1261 cctttatagt ggagtactac atttacgaga ttcggttaga gtatcagaaa aagaaaaaat
     1321 aaaagttaca gaaatgtata cttcaataaa tggtgaatta tgtaagattg atagagctta
     1381 ttctggagaa attgttattt tgcaaaatga gtttttgaag ttaaatagtg ttcttggaga
     1441 tacaaaacta ttgccacaga gaaaaaagat tgaaaatccg caccctctac tacaaacaac
     1501 tgttgaaccg agtaaacctg aacagagaga aatgttgctt gatgcccttt tggaaatctc
     1561 agatagtgat ccgcttctac gatattacgt ggattctacg acacatgaaa ttatactttc
     1621 tttcttaggg aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt
     1681 ggagatagaa ctaaaagagc ctacagtcat ttatatggag agaccgttaa aaaatgcaga
     1741 atataccatt cacatcgaag tgccgccaaa tcctttctgg gcttccattg gtttatctgt
     1801 atcaccgctt ccgttgggaa gtggaatgca gtatgagagc tcggtttctc ttggatactt
     1861 aaatcaatca tttcaaaatg cagttatgga agggatacgc tatggttgcg aacaaggatt
     1921 atatggttgg aatgtgacgg attgtaaaat ctgttttaag tacggtttat actatagccc
     1981 tgttagtact ccagcagatt ttcggatgct tactcctatt gtactggagc aagcctttag
     2041 aaaagctgga acagaattgt tagagccata tcttagtttt aaagtttatg caccacagga
     2101 atatctttca cgggcatata acgatgctcc caaatattgt gcaaatatcg taaatactca
     2161 actgaaaaat aatgaggtca ttattattgg agaaattcct gctcgatgta ttcaagatta
     2221 tcgcaatgat ttaacttttt ttacaaatgg gcttagtgtt tgtttagcag agctaaaagg
     2281 atatcaggtt accactggcg aacctgtttg ccagacccgt cgtctaaata gtcggataga
     2341 taaagtaaga tatatgttca ataaaataac ttagtgcgtt ttatgttgtt atataaatat
     2401 ggtttcttat taaataagat gaaatattct ttaatataga tttgaattaa agtggaaagg
     2461 aggagattgt tattataaac tacaagtgga tattgtgtcc tatttgtgga aataaaacaa
     2521 gactacgaat acgagtggat actatactta aaaatttccc tttatactgc cccaaatgta
     2581 agaacgaaac tttaattaat gttcaaaaaa tgaatataat aacaatcaaa gagccagacg
     2641 ccaagacgca gagccgataa tttgagaaat gaaactctca tcttatcggc tctttttgtt
     2701 tatctgaatt ttactgacta gccttcaata tttcttgctt cattgatacc ttttgctagt
     2761 gcttccatta aggatagttc tttgtctgta aagctatcca tgtatttttc tatctgtagc
     2821 cttcgagtgc tttttatcaa attatctgta ggtaagaaaa attcatcaat agacacatgg
     2881 agtaatgata caagatcata aagcacctgt gtacttgggt gttgcccttt attttctata
     2941 tttgttaaat aacgtgggtc aatttcaatg attgttccta cttgttcacg ggtgagacca
     3001 cgttttattc gagcctcttt aatcgctaga ccaacggctc taaaatcgta tttatcttct
     3061 tttttacgca taataaacca cctctataca ttttactgtt cctatccaag tggaaacagg
     3121 tatagaaaaa cgtgttatgt agtttgtgag ttcttttaag agcgctatta tgcttatgaa
     3181 tgtgataaat ccaatctcaa atagaaaggt cgctgatttt atttacccat acaactatat
     3241 aaaatttagt tatgctgcta aaaagtagat aaaattggcg actgaatgga ggtcacagtc
     3301 gccatgaaaa agatacgtca ctatctaatt ctcttaatta ggttcttatc aaaaaaactt
     3361 acccacctac actagataaa tatgaccatc aatgtggttt tacaaatcgt atagacagaa
     3421 caattgaagc acatgaacag agaaaacaat ctgtttatgt gtttttttgg ctacttcgtg
     3481 aatatctttt aagatttttc ttcacttatg caacaaatcc ccctttcgtg tcgggactta
     3541 gtatgaaggg agataaagaa gtacatgcca ctccctttca cgacagaaag ggggtgaaaa
     3601 acatgaaacc atcttctttt caagaaacca tagaaaatca gtttgactat atctgcaaac
     3661 aagccattga agatgaacgg aaagattatt tcaaacatct aagcagactg tccaaaaaag
     3721 aagtttcatt ttctgaaata gataactacc ttgtcacctc tttttctacg gttgatacgt
     3781 atgtcactga ttttcaacta ttcacattgt atggattaag aatcaatgtt gaaagtgatt
     3841 tgttaagcga agccttacgc caccttccag agaacaagcg gaacatcatt ttac
//
